LOCUS       DADLTD010000001        77352 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLTD010000001 DADLTD010000000
VERSION     DADLTD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.001"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1624
                     /locus_tag="JN701_000001"
     CDS             <1..1624
                     /locus_tag="JN701_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7362561.1"
                     /translation="NNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSY
                     STVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHN
                     GNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            1712..2335
                     /gene="iprA"
                     /locus_tag="JN701_000002"
     CDS             1712..2335
                     /gene="iprA"
                     /locus_tag="JN701_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HAY7362562.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(2336..3481)
                     /gene="ampH"
                     /locus_tag="JN701_000003"
     CDS             complement(2336..3481)
                     /gene="ampH"
                     /locus_tag="JN701_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY7362563.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQA
                     DRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGFITYM
                     AMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            3833..5053
                     /gene="sbmA"
                     /locus_tag="JN701_000004"
     CDS             3833..5053
                     /gene="sbmA"
                     /locus_tag="JN701_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY7362564.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            5066..6160
                     /gene="yaiW"
                     /locus_tag="JN701_000005"
     CDS             5066..6160
                     /gene="yaiW"
                     /locus_tag="JN701_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY7362565.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6219..6527)
                     /locus_tag="JN701_000006"
     CDS             complement(6219..6527)
                     /locus_tag="JN701_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY7362566.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            6787..6999
                     /locus_tag="JN701_000007"
     CDS             6787..6999
                     /locus_tag="JN701_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HAY7362567.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(7023..8117)
                     /gene="ddlA"
                     /locus_tag="JN701_000008"
     CDS             complement(7023..8117)
                     /gene="ddlA"
                     /locus_tag="JN701_000008"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY7362568.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            8195..8352
                     /locus_tag="JN701_000009"
                     /pseudo
     CDS             8195..8352
                     /locus_tag="JN701_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8579..8839
                     /gene="iraP"
                     /locus_tag="JN701_000010"
     CDS             8579..8839
                     /gene="iraP"
                     /locus_tag="JN701_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY7362569.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            8940..10355
                     /gene="phoA"
                     /locus_tag="JN701_000011"
     CDS             8940..10355
                     /gene="phoA"
                     /locus_tag="JN701_000011"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAY7362570.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            10474..10794
                     /gene="psiF"
                     /locus_tag="JN701_000012"
     CDS             10474..10794
                     /gene="psiF"
                     /locus_tag="JN701_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY7362571.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            10896..12011
                     /gene="adrA"
                     /locus_tag="JN701_000013"
                     /gene_synonym="dgcC"
     CDS             10896..12011
                     /gene="adrA"
                     /locus_tag="JN701_000013"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY7362572.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(12028..12837)
                     /gene="proC"
                     /locus_tag="JN701_000014"
     CDS             complement(12028..12837)
                     /gene="proC"
                     /locus_tag="JN701_000014"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY7362573.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            12957..13415
                     /locus_tag="JN701_000015"
     CDS             12957..13415
                     /locus_tag="JN701_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY7362574.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            13598..14122
                     /gene="aroL"
                     /locus_tag="JN701_000016"
     CDS             13598..14122
                     /gene="aroL"
                     /locus_tag="JN701_000016"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY7362575.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            14172..14363
                     /gene="yaiA"
                     /locus_tag="JN701_000017"
     CDS             14172..14363
                     /gene="yaiA"
                     /locus_tag="JN701_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY7362576.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            14621..15298
                     /locus_tag="JN701_000018"
     CDS             14621..15298
                     /locus_tag="JN701_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY7362577.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            15370..15654
                     /gene="ppnP"
                     /locus_tag="JN701_000019"
     CDS             15370..15654
                     /gene="ppnP"
                     /locus_tag="JN701_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY7362578.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            15802..16143
                     /locus_tag="JN701_000020"
                     /pseudo
     CDS             15802..16143
                     /locus_tag="JN701_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            16140..16223
                     /locus_tag="JN701_000021"
     CDS             16140..16223
                     /locus_tag="JN701_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362579.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(16301..17212)
                     /gene="rdgC"
                     /locus_tag="JN701_000022"
     CDS             complement(16301..17212)
                     /gene="rdgC"
                     /locus_tag="JN701_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY7362580.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            17337..18245
                     /gene="mak"
                     /locus_tag="JN701_000023"
     CDS             17337..18245
                     /gene="mak"
                     /locus_tag="JN701_000023"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY7362581.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(18388..19572)
                     /gene="araJ"
                     /locus_tag="JN701_000024"
     CDS             complement(18388..19572)
                     /gene="araJ"
                     /locus_tag="JN701_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY7362582.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPATGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(19698..22841)
                     /gene="sbcC"
                     /locus_tag="JN701_000025"
     CDS             complement(19698..22841)
                     /gene="sbcC"
                     /locus_tag="JN701_000025"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY7362583.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMARRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(22838..24040)
                     /gene="sbcD"
                     /locus_tag="JN701_000026"
     CDS             complement(22838..24040)
                     /gene="sbcD"
                     /locus_tag="JN701_000026"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY7362584.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            24230..24919
                     /gene="phoB"
                     /locus_tag="JN701_000027"
     CDS             24230..24919
                     /gene="phoB"
                     /locus_tag="JN701_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY7362585.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKCESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            24977..26272
                     /gene="phoR"
                     /locus_tag="JN701_000028"
     CDS             24977..26272
                     /gene="phoR"
                     /locus_tag="JN701_000028"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY7362586.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            26679..27998
                     /gene="brnQ"
                     /locus_tag="JN701_000029"
     CDS             26679..27998
                     /gene="brnQ"
                     /locus_tag="JN701_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY7362587.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            28074..29447
                     /gene="proY"
                     /locus_tag="JN701_000030"
     CDS             28074..29447
                     /gene="proY"
                     /locus_tag="JN701_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY7362588.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            29603..31325
                     /gene="malZ"
                     /locus_tag="JN701_000031"
                     /pseudo
     CDS             29603..31325
                     /gene="malZ"
                     /locus_tag="JN701_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(31330..31911)
                     /gene="acpH"
                     /locus_tag="JN701_000032"
     CDS             complement(31330..31911)
                     /gene="acpH"
                     /locus_tag="JN701_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAY7362589.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            32004..33074
                     /gene="queA"
                     /locus_tag="JN701_000033"
     CDS             32004..33074
                     /gene="queA"
                     /locus_tag="JN701_000033"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY7362590.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            33130..34257
                     /gene="tgt"
                     /locus_tag="JN701_000034"
     CDS             33130..34257
                     /gene="tgt"
                     /locus_tag="JN701_000034"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY7362591.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            34280..34612
                     /gene="yajC"
                     /locus_tag="JN701_000035"
     CDS             34280..34612
                     /gene="yajC"
                     /locus_tag="JN701_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY7362592.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            34640..36487
                     /gene="secD"
                     /locus_tag="JN701_000036"
     CDS             34640..36487
                     /gene="secD"
                     /locus_tag="JN701_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY7362593.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            36498..37469
                     /gene="secF"
                     /locus_tag="JN701_000037"
     CDS             36498..37469
                     /gene="secF"
                     /locus_tag="JN701_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY7362594.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            37598..37945
                     /locus_tag="JN701_000038"
     CDS             37598..37945
                     /locus_tag="JN701_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY7362595.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(38122..39006)
                     /locus_tag="JN701_000039"
     CDS             complement(38122..39006)
                     /locus_tag="JN701_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY7362596.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(39305..39844)
                     /locus_tag="JN701_000040"
     CDS             complement(39305..39844)
                     /locus_tag="JN701_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7362597.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            39995..40444
                     /gene="nrdR"
                     /locus_tag="JN701_000041"
     CDS             39995..40444
                     /gene="nrdR"
                     /locus_tag="JN701_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY7362598.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            40448..41551
                     /gene="ribD"
                     /locus_tag="JN701_000042"
     CDS             40448..41551
                     /gene="ribD"
                     /locus_tag="JN701_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY7362599.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            41640..42110
                     /gene="ribE"
                     /locus_tag="JN701_000043"
     CDS             41640..42110
                     /gene="ribE"
                     /locus_tag="JN701_000043"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY7362600.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            42130..42549
                     /gene="nusB"
                     /locus_tag="JN701_000044"
     CDS             42130..42549
                     /gene="nusB"
                     /locus_tag="JN701_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY7362601.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            42627..43604
                     /gene="thiL"
                     /locus_tag="JN701_000045"
     CDS             42627..43604
                     /gene="thiL"
                     /locus_tag="JN701_000045"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY7362602.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            43582..44100
                     /gene="pgpA"
                     /locus_tag="JN701_000046"
     CDS             43582..44100
                     /gene="pgpA"
                     /locus_tag="JN701_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY7362603.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(44154..45128)
                     /gene="yajO"
                     /locus_tag="JN701_000047"
     CDS             complement(44154..45128)
                     /gene="yajO"
                     /locus_tag="JN701_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY7362604.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGCALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(45183..47045)
                     /gene="dxs"
                     /locus_tag="JN701_000048"
     CDS             complement(45183..47045)
                     /gene="dxs"
                     /locus_tag="JN701_000048"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY7362605.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(47070..47969)
                     /gene="ispA"
                     /locus_tag="JN701_000049"
     CDS             complement(47070..47969)
                     /gene="ispA"
                     /locus_tag="JN701_000049"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY7362606.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(47969..48211)
                     /gene="xseB"
                     /locus_tag="JN701_000050"
     CDS             complement(47969..48211)
                     /gene="xseB"
                     /locus_tag="JN701_000050"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY7362607.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            48417..49865
                     /gene="thiI"
                     /locus_tag="JN701_000051"
     CDS             48417..49865
                     /gene="thiI"
                     /locus_tag="JN701_000051"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY7362608.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(49919..50509)
                     /gene="yajL"
                     /locus_tag="JN701_000052"
     CDS             complement(49919..50509)
                     /gene="yajL"
                     /locus_tag="JN701_000052"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY7362609.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(50472..51383)
                     /gene="panE"
                     /locus_tag="JN701_000053"
     CDS             complement(50472..51383)
                     /gene="panE"
                     /locus_tag="JN701_000053"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY7362610.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            51551..52042
                     /gene="yajQ"
                     /locus_tag="JN701_000054"
     CDS             51551..52042
                     /gene="yajQ"
                     /locus_tag="JN701_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY7362611.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(52170..53534)
                     /locus_tag="JN701_000055"
     CDS             complement(52170..53534)
                     /locus_tag="JN701_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7362612.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(53683..54573)
                     /gene="cyoE"
                     /locus_tag="JN701_000056"
     CDS             complement(53683..54573)
                     /gene="cyoE"
                     /locus_tag="JN701_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY7362613.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(54585..54914)
                     /locus_tag="JN701_000057"
     CDS             complement(54585..54914)
                     /locus_tag="JN701_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY7362614.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(54914..55528)
                     /locus_tag="JN701_000058"
     CDS             complement(54914..55528)
                     /locus_tag="JN701_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY7362615.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(55518..57509)
                     /gene="cyoB"
                     /locus_tag="JN701_000059"
     CDS             complement(55518..57509)
                     /gene="cyoB"
                     /locus_tag="JN701_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAY7362616.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(57531..58478)
                     /gene="cyoA"
                     /locus_tag="JN701_000060"
     CDS             complement(57531..58478)
                     /gene="cyoA"
                     /locus_tag="JN701_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAY7362617.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(58939..60414)
                     /gene="ampG"
                     /locus_tag="JN701_000061"
     CDS             complement(58939..60414)
                     /gene="ampG"
                     /locus_tag="JN701_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY7362618.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLTLWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(60458..61036)
                     /locus_tag="JN701_000062"
     CDS             complement(60458..61036)
                     /locus_tag="JN701_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7362619.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            61341..61658
                     /gene="bolA"
                     /locus_tag="JN701_000063"
     CDS             61341..61658
                     /gene="bolA"
                     /locus_tag="JN701_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY7362620.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            61709..61855
                     /locus_tag="JN701_000064"
     CDS             61709..61855
                     /locus_tag="JN701_000064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362621.1"
                     /translation="MKNGLRAVLFCLDFALFAQHSDENCPGIVKKYATARNNRSRLIK
                     VMPL"
     gene            62002..63300
                     /gene="tig"
                     /locus_tag="JN701_000065"
     CDS             62002..63300
                     /gene="tig"
                     /locus_tag="JN701_000065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY7362622.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            63545..64168
                     /gene="clpP"
                     /locus_tag="JN701_000066"
     CDS             63545..64168
                     /gene="clpP"
                     /locus_tag="JN701_000066"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY7362623.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            64294..65568
                     /gene="clpX"
                     /locus_tag="JN701_000067"
     CDS             64294..65568
                     /gene="clpX"
                     /locus_tag="JN701_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY7362624.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            65756..68110
                     /gene="lon"
                     /locus_tag="JN701_000068"
     CDS             65756..68110
                     /gene="lon"
                     /locus_tag="JN701_000068"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY7362625.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            68319..68591
                     /gene="hupB"
                     /locus_tag="JN701_000069"
     CDS             68319..68591
                     /gene="hupB"
                     /locus_tag="JN701_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY7362626.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            68783..70654
                     /gene="ppiD"
                     /locus_tag="JN701_000070"
     CDS             68783..70654
                     /gene="ppiD"
                     /locus_tag="JN701_000070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7362627.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYHALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            70805..71176
                     /locus_tag="JN701_000071"
     CDS             70805..71176
                     /locus_tag="JN701_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362628.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            71270..71668
                     /locus_tag="JN701_000072"
     CDS             71270..71668
                     /locus_tag="JN701_000072"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY7362629.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(71720..72415)
                     /gene="queC"
                     /locus_tag="JN701_000073"
     CDS             complement(71720..72415)
                     /gene="queC"
                     /locus_tag="JN701_000073"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY7362630.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(72480..74180)
                     /locus_tag="JN701_000074"
     CDS             complement(72480..74180)
                     /locus_tag="JN701_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY7362631.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            74280..75062
                     /gene="cof"
                     /locus_tag="JN701_000075"
     CDS             74280..75062
                     /gene="cof"
                     /locus_tag="JN701_000075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY7362632.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTH"
     gene            75164..75709
                     /locus_tag="JN701_000076"
     CDS             75164..75709
                     /locus_tag="JN701_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY7362633.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILCGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            75739..>77352
                     /locus_tag="JN701_000077"
     CDS             75739..>77352
                     /locus_tag="JN701_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7362634.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEAS"
BASE COUNT        18881 a        19166 c        20569 g        18736 t
ORIGIN      
        1 aaataatggc tctcaggtaa atgttactga ttcctctctg gtatcagata ccattggttt
       61 aaccaacggt tcttcactga atattggtgc aaatggcgtg gttgctacag accacctgac
      121 tgttgatagc tacagtaccg ttaatttaac cgaatctacc ggttggaata attattctaa
      181 tctgtatacc aatactatca ctgtgactaa cggtggtgtg ctggatgtga atgttgatca
      241 gttcgatact gaagctttcc gtactgataa actggaactg accagcggca acattgctga
      301 ccataatggc aacgtaattt ctggtgtgtt cgacatcaac agcagcgatt acgttctgaa
      361 cgctgatctg gtgaacgacc gtacgtggga tacttccaag tctaactacg gttacggtat
      421 tgttgctatg aactctgatg gtcacctgac tatcaatggt aacggcgacg tagacaacgg
      481 tactgaactg gataacagct ctgttgataa cgttgttgct gcaaccggta actacaaagt
      541 tcgtatcgac aacgcaactg gcgctggcac tatcgctgat tacaaagata aagaacttat
      601 tcgggtaaac gacgctaaca ccaacgcgac cttctctgct gctaacaaag ctgacctggg
      661 tgcatacacc tatcaggctg aacagcgcgg taacaccgtt gttctgcaac agatggagct
      721 gaccgactac gctaacatgg cgctgagcat cccgtctgcg aacaccaata tctggaacct
      781 ggaacaagac accgttggta ctcgtctgac caactctcgt catggcctgg ctgataacgg
      841 cggcgcatgg gtaagctact tcggtggtaa cttcaacggc gacaacggca ccatcaacta
      901 tgatcaggat gttaacggca tcatggtcgg tgttgatacc aaaattgacg gtaacaacgc
      961 taagtggatc gtcggtgcgg ctgcaggctt cgctaaaggt gacatgaatg accgttctgg
     1021 tcaggtggat caagacagcc agactgccta catctactct tctgctcact tcgcgaacaa
     1081 cgtctttgtt gatggtagct tgagctactc tcacttcaac aacgacctgt ctgcaaccat
     1141 gagcaacggt acttacgttg acggtagcac caactctgac gcgtggggct tcggtttgaa
     1201 agccggttac gacttcaaac tgggtgatgc tggttacgag actccttacg gcagcgtttc
     1261 tggtctgttc cagtctggtg atgactacca gctgagcaac gacatgaaag ttgacggtca
     1321 gtcttacgac agcatgcgtt atgaactggg tgtagatgca ggttatacct tcacctacag
     1381 cgaagatcag gctctgactc cgtacttcaa actggcttac gtctacgacg actcaaacaa
     1441 cgataacgat gtgaacggtg attccatcga taacggtact gaagggtctg cggtacgtgt
     1501 tggtctgggt actcagttca gcttcaccaa gaacttcagc gcctataccg atgctaacta
     1561 cctcggtggt ggtgacgtag atcaagactg gtccgcgaac gtgggtgtta aatatacttg
     1621 gtaatattct tcactccgaa gaaattctgg taatttaatc taaataatgc ccgtcaagga
     1681 tttgacgggc attactgcaa aggacgcgca aatgttatct gtagttaaac ctcttcagga
     1741 atttggtaag ctcgataaat gtttgtccag atacggtacg cgcttcgagt ttaataatga
     1801 aaagcaagtt atattttcca gtgatgtcaa tagcgaagat actttcttta ttttagaggg
     1861 cgttatctct ctgcgtagag aagaaaacgt acttatcggt attacccagg cttcttatat
     1921 tatggggctg gctgatggtt taatgaaaaa cgatatacca tacaaattaa tatcagaagg
     1981 aaattgtacg ggatatcatc taccagccaa acaaaccatt acgcttattg aacaaaatca
     2041 actctggcga gacgcttttt actggttagc ctggcaaaat agaattctgg aattacgcga
     2101 cgtgcagctc attgggcata attcctacga acaaatccgc gcaacattat tatcaatgat
     2161 tgactggaat gaagaattgc gatcacgtat tggtgtgatg aattatatcc atcaacgtac
     2221 acgcatatcg cgttctgtcg tcgcagaagt tctcgctgct ttgcgtaaag gcggctatat
     2281 cgaaatgaat aaaggcaaac tggtcgctat caaccgtttg ccttcagagt attaatcagg
     2341 atgcagggat aaccaacggt ttattcccgc ttagctcggt caccagatca ttgatgccat
     2401 cgctcatatt tttaaagcgc gtcaacggcg aacgagtgac caccacaaac gcgccaatat
     2461 tcttctgcgg gatcatcgcc atataggtaa tgaaaccgcc accaccgcct gtcttctgaa
     2521 taatccccgg acgaccctct ttcggggcca tatataccca gcctaaacca agcgcatccg
     2581 ctttgccggg gacatccatg ccaatcactt tggtaaactg cgcgcgctga taaatcaacg
     2641 tctgcatgcg atctgcctga taaaaatcag atgataaata ctgctgcatc cagcgcatca
     2701 tatcgccagg cgtggaatag acgccgccgc tgccaattgc cgccagcgtg ttattgcacg
     2761 gactggcacc gcgctcagca accattaaac ggcggcactg atccggtgag ggggtgtagg
     2821 tggtgtcttt catccccaat ggacgggtaa tctgctcttc aaacaactgg gtataaggct
     2881 tgccggacgc attcgccagt gcatctgcca gcaggtcgaa cgcaaggtta gagtacgccg
     2941 cctgactgcc tggcgcggct ttcagcttcg ccgtagaaag gtatttccag cgttgctcgc
     3001 gcgttggcca gacaaacacc ggacgatgtg ccgcgccacc gggctgttca cggggcaggg
     3061 cgctggtatg ggttgccaga ttcactaggg taatgggcgt cccattgtag gttggcacgc
     3121 gtgcgcctgg cggagcatat ttacttagcg gatcgtttaa cttcacggtc ccctggtcga
     3181 gcaatttcac cagcatttca ctggtcatta gcttggtgag ggaagcaata cgcacgaccg
     3241 aatccagctg cgggcgaacg ttattaccag gtcgcgtttc gccataactg cgaaagacgc
     3301 gctggttacc gtcgataaca accagcgcca tccccgtggc accgctgccg taaaaaatat
     3361 gatcggcata acgatcgaca atatcagagg caaactccgg ttcagtgatc ggctgcgccg
     3421 catggacgga ggtcaatgac gccgcacaca gcacggcaga aaaaagcaga ctacgtttca
     3481 acggtggtgt ccatagattg aatgaaggta actgagcgta tttatactac tcattgatgg
     3541 attgcaaagg gcgaattagt gcaataatag tgttaaaaac gtaacggcgc gtaaacacga
     3601 tctttttcct gctcaccaat gcctgcattt gtgatcctgc gcgtgttaat ccgcttatgt
     3661 aaacaatctt tggtttgtga aatccgtcga aacaattctt atggtcaggc tggcgaacta
     3721 agcgccttgc tatgggtcac aatgggcggt ttcatttggc tgacgctttg tagtggtcat
     3781 gcggttaata cacagcagtt gttaaaacga taagaagtta gcaggagtgc atatgtttaa
     3841 gtcttttttc ccaaagccgg gagcgttttt tctctcggcc tttgtttggg cattgattgc
     3901 cgttatcttc tggcaagccg gtgggggaga ctgggtggcg cgtatcaccg gcgcttccgg
     3961 gcagatcccg attagcgccg cgcgtttctg gtcgttggat ttcctgattt tttacgctta
     4021 ctacattgtt tgcgtaggac tttttgcatt tttctggttt atctacagcc cgcaccgttg
     4081 gcaatactgg tcaatactcg gtactgcact gatcatcttc gtcacctggt ttttggtgga
     4141 agtcggagtc gccgtcaatg cctggtatgc accgttctat gatctgattc aaaccgcgct
     4201 aagttcgccg cataaagtca ctatcgaaca attttaccgc gaagtgggcg tctttctggg
     4261 gattgcgctg atcgcggtgg tgatcagtgt actgaacaac ttctttgtca gtcactatgt
     4321 gttccgctgg cgtacggcga tgaacgaata ttacatggcg aactggcaac aactgcgtca
     4381 tatcgaaggt gccgcacagc gtgtgcagga agacaccatg cgttttgctt caacgctgga
     4441 gaatatgggc gtcagtttta tcaacgccat catgacgttg gtcgccttcc tgccggtgct
     4501 ggtaacgctc tccgcgcatg tgccggagct gccgattatc gggcacattc cgtatggtct
     4561 ggtgattgcc gctatcgtct ggtcgctgat ggggaccgga ttgctggcag tggtagggat
     4621 caaactgccg gggctggagt ttaaaaacca gcgagtagag gccgcctacc gtaaagagct
     4681 ggtttatggt gaagacgatg ccacgcgcgc gacgccgcct acggtacgcg agctgtttag
     4741 cgccgtacgg aaaaactatt ttcgcctcta ttttcactat atgtatttca acatcgcccg
     4801 cattctctat ttgcaggtcg ataacgtttt cggtttgttc ttgctgtttc cgtcaattgt
     4861 tgccggtacg attacgctcg gcctgatgac gcagattacc aacgtttttg gtcaggttcg
     4921 cggtgctttc cagtacctga ttaactcatg gaccacactg gttgagttga tgtctatcta
     4981 caaacgtctg cgcagctttg aacatgagct ggatggtgac aaaattcagg aagtaaccca
     5041 taccttgagc taaaaaggag aagtaatgtc gcgcgtcaat catctatctt cgctgtcatt
     5101 gttggcggtt ctggttttag cggggtgtag tagtcaggct ccgcaaccgt tgaaaaaggg
     5161 cgaaaaagcg atagatgtag cgagcgtcgt gcggcagaag atgcctgcga gcgtgaaaga
     5221 ccgcgatgcc tgggcgaaag atctcgccac cacctttgaa agtcaggggc tggcaccgac
     5281 gctggaaaac gtctgctcgg tgctggcggt ggcgcagcag gagtcaaatt atcaggcaga
     5341 tccggccgtt ccgggcttaa gcaagatcgc ctggcaagag attgaccgtc gtgctgaacg
     5401 gatgcacatt cctgcttttc tggttcatac cgcgctaaaa atcaaatcgc caaacggcaa
     5461 aagttatagc gaacggttag attcggtgcg tacagaaaag caattgagcg cgatttttga
     5521 cgatctgatc agcatggtac caatggggca gacgctgttt ggttcgctta atccggtgcg
     5581 caccggtggg ccgatgcagg tcagcattgc ttttgccgaa cagcatacca aagggtatcc
     5641 gtggaaaatg gacggtacag ttcgtcagga agtcttcagt cgccgcggcg ggttgtggtt
     5701 tggtacttac catttactga attatcccgc cagctatagc gcaccgatat accgctttgc
     5761 tgattttaac gctggctggt acgccagccg taatgccgcg tttcagaacg cggtcagtaa
     5821 ggccagcggc gtgaagctgg cactggacgg tgatttaatt cgttacgaca gtaaagaacc
     5881 cgggaaaacc gaactggcaa cgcgcaaact ggcgggaaaa ctggggatga gcgacagcga
     5941 aattcgccgt cagttagaga aaggtgacag cttctctttt gaggagacgg cgctgtacaa
     6001 gaaagtttat caacttgccg aagcgaaaac gggtaaatca ctaccccgcg aaatgttgcc
     6061 tggcattcaa ctggaaagcc cgaaaatcac ccgcaacctg actacggcct ggtttgcgaa
     6121 gcgcgtagac gaacggcggg cgcgttgtat gaaacagtga tcaaacagga atgtcatgcc
     6181 agataaggcg ttttaggccg tatctggcaa cgtaaatctt actggcggcg atgtcgccag
     6241 tgcagccata gcgccaccac tgcaaaaatg aggcagccta ctaaaaacgg gatcagcccg
     6301 aaaatggtgc cgacacctaa accaatttcc acccgtgggc gacctgtttc ctggacctgc
     6361 attaaacgct catagacgcc gggcgcatgc actaacaggt tcagcaattg cgccccagcc
     6421 caaaagcaaa acagcacaaa caacgcatag gcaatgttgc caggcgttga agaggcattg
     6481 cgatattttc cgctaaacag cgattttgac agggtgaaat cagccataaa aacctccgga
     6541 agtcatctgc ttctcttctg gtgtgatacc agcggcttat ggcaagtctg acaggtcatt
     6601 tagattgtca atatcagaat catggtaaat tgatgttggg aatattcccg aagcgtgaat
     6661 cttcgttgtg agtcacaaaa tggcaacctt aagttaaatt tattaacaat tacgaaaatg
     6721 ttgtctacac cacagacggt tgttacagaa tatgcaagga tgttgttttt cgttaacgga
     6781 gctgccatga atctgcctgt aaaaatccgc cgtgactggc actactatgc gttcgccatt
     6841 ggccttatat tcattcttaa tggcgtggtg gggttactgg gatttgaagc aaaaggttgg
     6901 cagacctatg ccgtcggtct ggtgacgtgg gtgattagtt tctggctggc ggggttgatt
     6961 attcgtcgtc gcgatgaaga aactgaaaac gcccaataag aatattatcg ggcgttaaaa
     7021 tattacattg tggttttcaa tgcgttatcc gcagcgtgac gctccagcgc cagctcaatc
     7081 aaacgtgtga tcagatcggt gtaacccaga ccgctggctt gccacagctt cggatacata
     7141 ctgatgttgg tgaagccagg cagtgtgttg atctcgttga tcaccacttc gttctctggg
     7201 gttaaaaaca cgtctacacg cgccatgcct gcgcatccca acgtttgata agcctgaacg
     7261 gcaatcgccc ggatcttatc gttgatttct ggcgcaatgg ctgccggaac taccactttc
     7321 gcgccatctt cgtcaatgta cttggtgtcg taggcataga agtcgctggt gagtacgatc
     7381 tcgccacagg tgctggcttg cgggttgtcg ttgcccagaa ctgcgcattc gatctcacga
     7441 cctttgatcc cttgctcaac gatcacttta tgatcgaatt cgaacgccag atcgacggca
     7501 attgcgtact gttcttcact ggttactttg ctgacaccaa cagaagagcc ctgattagcc
     7561 ggttttacaa acagtggtaa cctcagttta gactccactt cggcaaaact aatgttgtga
     7621 cgattggcgc gcgtcagggt aataaatggc gcaatgttca gcccggcatc gcgcagcaga
     7681 cgtttggtaa catctttatc catacaggct gctgaagcca gaacatcaga acctacaaac
     7741 ggtaaattgg cgacccgcag cattccctgc aaggaaccat cttcgcccag cgtaccgtgg
     7801 acaatcggga aaatgacatc caccgtcggt aacggctgac cgttttgcgc gtcgataagc
     7861 tgatgctcat gtttacttgg cacctgcgca aggctagtcg ccgaagggcg caacgcaata
     7921 tgggcaggat cgtctgcatt tagcagataa ttgctggcat cgctgacgtg ccattgccct
     7981 tgtttatcaa tgcccagcag cacaacgtcg aagcgacttt tatcaatggc atcgacaatg
     8041 ttttttgccg attgcagaga cacttcatgt tccgctgatt taccaccaaa aacgattcct
     8101 acccgcagtt tttccatctt aaaaacctat cccgtctaac acaaagtgca tacattacca
     8161 cgacaaaacg ggggattcgc ggccttctga cagaatgttg caatcttctg ctgacaaagc
     8221 gtgcaacgta atggtgaaga aagtgcgtta ttacaaagat gtgcgcgaga tcacaaaaat
     8281 gatgaacggg aagctaattt attcctggct taaatggcca tgcggtgagt tttttctctt
     8341 agttgtaagt aaacgcagag aatatatttc ataacttttg tttataataa aggttgataa
     8401 ttaaaggcct gttctttgtg tggttaatta tttaaatatg agaaacgttt cgctggtaat
     8461 caaacaaaga acatttgcgc aaagtatttc ctttgtcata aaaataatac ttccagacac
     8521 tatgaagttg tgaaacataa tgttaacttc tccatacgtt ggataagaaa atacagacat
     8581 gaaaaatctc attgctgagt tgttattaaa gcttgcccaa aaagaagaag agtcgaaaga
     8641 actgtgcgcg caggtagaag ctttggagat tatcgtcact gcaatgcttc gcaatatggc
     8701 acaaaatgac caacagcgct tgattgaaca ggtggagggg gctctttacg aggtaaagcc
     8761 tgatgccagc attcctgacg acgatacgga gctgctgcgc aattatgtaa agaagttatt
     8821 gaagcatcct cgtcagtaaa aagttaatct tttcaacagc tgtcataaag ttgtcacggc
     8881 cgagacttat agtcgctttg tttttatttt ttaatgtatt tgtacatgga gaaaataaag
     8941 tgaaacaaag cactattgca ctggcactct taccgttact gtttacccct gtgacaaaag
     9001 cccggacacc agaaatgcct gttctggaaa accgggctgc tcagggcgat attactgcac
     9061 ccggcggtgc tcgccgttta acgggtgatc agactgccgc tctgcgtgat tctcttagcg
     9121 ataaacctgc aaaaaatatt attttgctga ttggcgatgg gatgggggat tcggaaatta
     9181 cagccgcacg taattatgcc gaaggtgcgg gcggcttttt taaaggtatc gatgccttac
     9241 cgcttaccgg gcaatacact cactatgcgc tgaataaaaa aaccggcaaa ccggactacg
     9301 tcaccgattc ggctgcatca gcaaccgcct ggtcaaccgg tgtcaaaacc tataacggcg
     9361 cgctgggcgt cgatattcac gaaaaagatc acccaacgat tctggaaatg gcaaaagccg
     9421 caggtctggc gaccggtaac gtttctaccg cagagttgca ggatgccacg cccgctgcgc
     9481 tggtggcaca tgtgacttcg cgcaaatgct acggtccgag cgcgaccagt gaaaaatgtc
     9541 cgggtaacgc tctggaaaaa ggcggaaaag gatcgattac cgaacagctg cttaatgctc
     9601 gtgccgacgt tacgcttggc ggtggcgcaa aaacctttgc tgaaacggca accgccggtg
     9661 aatggcaggg aaaaacgctg cgtgaacagg cacaggcgcg tggttatcag ttggtcagcg
     9721 atgccgcctc gctaaatgcg gtgacggaag cgaatcagca aaaacctctg cttggactgt
     9781 ttgctgacgg caatatgcca gtgcgctggc taggaccgaa agcaacgtac cacggcaata
     9841 tcgataagcc cgcagtcacc tgtacgccta atccgcaacg taatgacagt gtaccaaccc
     9901 tggcgcagat gaccgacaaa gccattgaat tgttgagtaa aaatgagaaa ggatttttcc
     9961 tgcaagttga aggtgcgtca atcgataaac aggatcacgc tgcgaatcct tgtgggcaaa
    10021 ttggcgagac ggtcgatctc gatgaagccg tacaacgggc gctggaattc gctaaaaagg
    10081 atggcaacac gctggtcata gtcaccgctg atcacgccca cgccagccag attgtcgcgc
    10141 cggataccaa agctccgggc ctcacccagg cgctaaatac caaagatggc gcagtgatgg
    10201 tgatgagcta cgggaactcc gaagaggatt cacaagaaca taccggcagt cagttgcgta
    10261 ttgcggcgta tggcccgcat gccgccaatg tcgttggact gaccgaccag accgatctct
    10321 tctacaccat gaaagccgcc ctggggctga aataaaaccg cgcccggcag tgaattttcg
    10381 ctgccgggtg gtttttttgc tgttagcaac cagacttaat ggcaaatctc gggcgcatac
    10441 gctcatggtt aaaacatgaa gagggatggt gctatgaaaa ttacattact ggttaccttg
    10501 cttttcggtc tggttttttt aaccacagtc ggcgctgccg agagaacgtt aaccccacaa
    10561 caacagcgta tgacctcctg taatcagcag gcgacggcgc aggcgttgaa aggggatgct
    10621 cgtaagacct acatgagtga ttgcctgaag aacagcaagt ctgcgcctgg tgaaaaaagt
    10681 ttgacgccac agcagcaaaa gatgcgcgaa tgtaataatc aagcaacgca acaatctctg
    10741 aaaggtgatg atcgtaataa gtttatgagt gcctgcctca agaaagccgc ctgatacctg
    10801 atagtgctaa cgggtgagct acgaaagtgg ctcacccgaa atttcatact tctgccttta
    10861 gccccgtctc tataatttgg gaaaattgtt tctgaatgtt cccaaaaata atgaatgatg
    10921 aaaacttttt caaaaaagcg gcggcgcatg gggaggaacc tcctttaact cctcaaaacg
    10981 aacatcagcg gtccgggctg cgcttcgccc gtcgcgtcag actaccccgt gcggttggcc
    11041 tggctggcat gttcttaccg attgcttcaa cgctggtttc gcacccgccg ccgggctggt
    11101 ggtggctagt gttggtcggc tgggcgttcg tctggccgca tttagcctgg cagatagcga
    11161 gcagggccgt cgatccgctt agccgggaaa tttacaactt aaaaaccgat gcagtattag
    11221 cgggaatgtg ggtaggcgta atgggcgtaa acgtgctgcc ttccaccgct atgttgatga
    11281 ttatgtgtct gaatttgatg ggggcaggcg gcccccgtct gtttgtcgcg ggtctggtgt
    11341 tgatggtggt ttcctgcctt gtcaccctcg agcttacggg cattaccgtg tcgttcaata
    11401 gtgcgccgct ggaatggtgg ctctcccttc ccattattgt cgtttatcct ctgctgtttg
    11461 gctgggtcag ctaccagacg gcaaccaaac tggcggaaca taaacgcagg ttgcaggtca
    11521 tgagtacccg cgacggcatg acgggcgtgt ataaccgacg tcattgggaa actatgttac
    11581 gcaatgaatt tgataactgt cggcggcata atcgcgatgc aacgttactg attatcgata
    11641 tcgaccattt caagagcatc aacgatacct ggggtcatga tgtgggcgat gaagcgattg
    11701 tggcgcttac ccgacagtta caaattaccc tgcgcggtag cgatgtgatt ggtcggtttg
    11761 gcggcgatga gtttgcagta atcatgtccg gtacgccagc tgagagcgcc attaccgcca
    11821 tgttacgggt gcgtgaaggg ctaaacacgt tacgcttgcc gaatacgcca caggtaactt
    11881 tacggattag tgtgggggtt gcgccgctga acccacaaat gagtcactat cgtgagtggt
    11941 tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac cgcaccgaag
    12001 tggcggcctg acgtcaggcg aaagtcatca ggatttgctg agtttttctg atttttccat
    12061 acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca gtacgcgtac
    12121 cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc ccggatgttc
    12181 tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa atttatacgc
    12241 ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga taaacataaa
    12301 tacgtaggct ggcgaagaac cgctcacacc gactaccggg tggatcatcg gctcagcaat
    12361 tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat cttctggggt
    12421 taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg gagtgttcgg
    12481 catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt cgagcgtgac
    12541 acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga tttcgctaag
    12601 cactttaatc atgatgccag gtttaacggc agcaaaaatg atgtcggcga tttgcgccac
    12661 ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg cgactttatt
    12721 cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg caatcagacc
    12781 gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct tttccattgc
    12841 ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat aaacagaact
    12901 atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat gactttatga
    12961 caatttgggt ggatgctgac gcgtgtccca atgtaattaa agagattttg tatcgcgcgg
    13021 cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc gtgccgccat
    13081 cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat aacgaaattg
    13141 tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg gctgctgaag
    13201 ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg ccagcgacca
    13261 ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt gggatccaga
    13321 ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg gagctggaga
    13381 agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt tacacttcat
    13441 tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta agtggaattt
    13501 tttctttaca atcgaaattg tactagtttg atggaatgat cgctattctc atgacaccgg
    13561 ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc tttttctgat
    13621 cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt cgcttaaccg
    13681 tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg tcgcggagat
    13741 cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc tggaagcggt
    13801 aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg aatttaatcg
    13861 tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat cagtcctggt
    13921 taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg gaaaaccgct
    13981 gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg aagttgcgca
    14041 tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc gtagcgccct
    14101 ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat tccgtgaaat
    14161 aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata gtgacgattg
    14221 aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat catcctaaac
    14281 cagactcgtt gatcagtgaa catccgaccg ctcaggaagc gatggatgcg aaaaaacgct
    14341 atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc tgagtgccac
    14401 tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt agaatattaa
    14461 acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt gcacgtctgc
    14521 tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct tcacacttgc
    14581 ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt cgttggcgat
    14641 cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg aatacattga
    14701 cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag aagtgatggc
    14761 ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg acaaccagct
    14821 ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg aagtgctcga
    14881 taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta gtatgactgc
    14941 gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt cctctattgt
    15001 tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgcccg ttcaggcgca
    15061 aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca ttcatgattc
    15121 agaacaaaaa atcattgatg cagggaaaga attactggca aaaggggctg atgtcatcat
    15181 gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac agctcgatgt
    15241 tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac tggtgtaatt
    15301 ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac taccagacag
    15361 ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc aatcggcttt
    15421 tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga atacaccttc
    15481 agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt gttactgcct
    15541 gatgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc cggtcacagt
    15601 gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct gtaattcctc
    15661 gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac ccataataat
    15721 atttcctttg ctgcgatttt ttcaatttcc gatatattca taattcatca aggttgatat
    15781 aaatatcagt gaagatctcc agatattgtt gcggaactgg ctacgataaa agataaatca
    15841 gatgatgaat ggtggcgtgc attgctgcaa agtcgcaatg atcatttgcg ccaaacggca
    15901 ttacgcaacg cacatacccc ggcgtcgttg ttaacgacat tgaccgagtc tcggcatcgg
    15961 tcgctagcta tgaataatcc acagctcgct gccgatgtga aaacgacgtg gttaaaagag
    16021 gatccatcat tactcttatt tgtcgaacaa cccgatcttt cgctgttacg ggatttagtg
    16081 aaaaccgggg caatgcggaa aattcgcagt gaagcgcgtc actggcttga ggaaaaacaa
    16141 tgacacaacg tccatggagc aagttacagc ataaaacgca taatattgct gcgctaaaaa
    16201 ttattgtccg ccgtagcgaa taattatatc cctggtgtgg cttcgtacgc cggataagac
    16261 gcggcaggcg tcgcatccgg cattaaagga aaatcagcaa ttaacgttgt gcttcgccac
    16321 ctaatccttc aatcaggttt tgaattaacg ctgccagttc accagtcatc aggatgaaat
    16381 cggcatcaaa acgctgggcg aaatcttcac ggtcgatatc ttcgttttga tcgcgcagct
    16441 cgtcgcaaaa cttcagacgc ttgagcgaac catcgtcgca catcacaaac tgaatgcgct
    16501 gctgccagtc gagcgccagt ttagtcacca cttttccggc ttcgatgtga ttggtgatct
    16561 cttcgctggt cagatcttgt ttcttcgcgc ggatcacgcc gccatcttcc agcaacgatt
    16621 tcagctcggc ttcatcaagc agctggaagc cctgtgccgc gctaccggag cgaacccatt
    16681 cggtcagcgt cagttcaatc gggttttcca tgctcaatgg taccacaggt aacgacccca
    16741 ggcttttacg cagcagtgcc agcgtatctt ccgctttttt ggcactggcg cagtccacca
    16801 taatcaaacc gttaaccgtg tcgatccaca tcattgtctg gctaaaacgg ctgaaagcac
    16861 gcggcaacag agagtgcagc acttcgtctt tcagcgaatc tttttcggtt ttcttcagct
    16921 tacgcgcctg ttccgcttcc agtttggcga ttttcgcttc cagcgcctgc ttaatcaccg
    16981 gagacgggag gattttttct tctttgcgcg cgcagataac aatttgacca ttggcaacgt
    17041 gcgttaacgc atcgctgtgc gatcccatcg gaggaaccca gcccatcttc gccatgtcct
    17101 ggctgccgca tggggtaaat gccatcgagg ctagctgttt ttccatctct tctgcacgca
    17161 gcgaaatctc gcggctaaga cggtaaacca ttaaattttt gaaccacagc atgataattt
    17221 ccacggcctt gttgttaaat ttagcgggca tgataacgaa ttgtcggcgg ccttgcattg
    17281 ccaatccggt tgtccgtctc tacgctattg atattgaaaa aaataaggag agtaccgtgc
    17341 gtataggtat cgatttaggc ggcaccaaaa ctgaagtgat tgcactgggc gatgcagggg
    17401 agcagttgta ccgccatcgt ctgcccacgc cgcgtgatga ttaccggcag actattgaaa
    17461 cgatcgccac gttggttgat atggcggagc aggcgacggg gcagcgcgga acggtaggta
    17521 tgggcattcc tggctcaatt tcgccttaca ccggtgtggt gaagaatgcc aattcaacct
    17581 ggctcaacgg tcagccattc gataaagact taagcgcgag gttgcagcgg gaagtgcggc
    17641 tggcaaatga tgctaactgt ctggcggttt cagaagcagt agatggcgcg gcagcgggag
    17701 cgcagacggt atttgccgtg attatcggca cgggatgcgg cgcgggcgtg gcattcaatg
    17761 ggcgggcgca tatcagcggc aatggcacgg caggtgagtg gggacacaat ccgctaccgt
    17821 ggatggacga agacgaactg cgttatcgcg aggaagtccc ttgttattgc ggtaaacaag
    17881 gttgtattga aacctttatt tcgggcacgg gattcgcgat ggattatcgt cgtttgagcg
    17941 gacatgcgct gaaaggcagt gaaattatcc gcctggttga agaaagcgat ccggtagcgg
    18001 aactggcatt gcgtcgctac gagctgcggc tggcaaaatc gctggcacat gtcgtgaata
    18061 ttctcgatcc ggatgtgatt gtcctggggg gggggatgag caatgtagac cgtttatatc
    18121 aaacggttcc gcagttgatt aaacaatttg tcttcggcgg cgaatgtgaa acgccggtgc
    18181 gtaaggcgaa gcacggtgat tccagcggcg tacgcggcgc tgcgtggtta tggccacaag
    18241 agtaaaaaac gtaggcaatt ggcgcatcat gcctgatgcg acgcttgccg cgtcttatca
    18301 ggcctgcaaa atgtgccaga accgcgtagg gcggataagg cgttcacgcc gcatccggca
    18361 ataagtaatg aacaccgaga ctataaccta ccccagtggt ttcgccagca ccggagtatc
    18421 cgccgcttgc tggcgcttat agcgaccata cagcagcaac gacgacatcg cagcaaacga
    18481 aagcagggcg gcaggcagcg ccacgtaatt atatgccagc cccagcgtca gcatcatacc
    18541 accgcaatat gcgccgacgg cgctaccgag gttaaacgct atttgcccac ctgcggcacc
    18601 taataactct ccgcctttgg cgttttgtag taacaatatt tgtagcggtg ctgaaagggc
    18661 aaataatccc gcgcaacaaa taaaagcaaa aataagcgac gttgttttca tgccgccgca
    18721 gaaaaagaac atcagcagtg ccagtacaat tataaagtca gtcactgctg caatgcgcag
    18781 tggtgaataa cgtcctgaaa tcctgccact tagcatattt cccagcacca tccctagccc
    18841 aactaacatc ataataaagg tcatcgccgt ttccgaaaaa ccggaaataa acatcatgta
    18901 tggctttacg tagctgaacc aggcaaacac acctgcgttg ccaaacatgg tggcggcgaa
    18961 aattaaccac ggggccgggc tgcgcaaaaa gtgaaattgt tcgcgcagct ttcctttcgc
    19021 ctcgtcgcga atatccggca cccaaaaata gaccgatgcc atcaccgcaa tattaaaaac
    19081 agcgatcaat aaaaaggtgt aacgccagct aaattcctga cttaaatacg ttcccagcgg
    19141 aatgcccagc aaattggcga ctgtcatccc ggaaaccatc cccgccacgg cggcggtgac
    19201 ttttccgggt ttgataattt ttgataacac gatcgcgccg acgccaaaaa atgcgccatg
    19261 cggaaagccg gataccagcc gaccaatggc gagcatcagg taagacgaag agagcgtgaa
    19321 catggcgttg ccaatgacgc acaacgccac cagaaacaac aagatatgtt tgagtgagta
    19381 gcggctggaa aagagtgcga tgattggcgc accgaccacc actcccagtg cataatacga
    19441 gatcatatgc ccggtagcag gaatcgaaat tcctacgtta tgcgccagct ccgtgagcac
    19501 gcccataatg ccaaattcgg ccatccccaa accaaacgtg cccagagcca aagataaaat
    19561 gacttttttc ataccaccat cacaaccacc agaacggcta ccaatcaccg tagccaataa
    19621 agtgatagct tgcaggataa cgaccagcaa ggccaagaga taattcgccc ctctgtattc
    19681 attatcctgc tgaatggtta tttcactgca aacgtacttt ccagtttgct gtagcccagg
    19741 ccgttgatct ttttcacttt gatctgcacc ggaatacgct ctttcatcgc ttctacgtgg
    19801 ctaatcacac cgatggtttt gccactggcg ttcagggcat ccagcgcatc aagggcggta
    19861 tccagcgttt cgctatccag cgtgccaaaa ccttcatcaa ggaacagcga gtcaatacgt
    19921 gttttatggc tgaccagatc cgaaagcgcc agcgccagcg ccagactaac gaggaaactt
    19981 tcgccgccgg aaagggtacg ggtatcgcgt accgcatctg cctgccaggt atcaacaacc
    20041 tcgacttcca gcgcctcgct ggctttgcgc tgtaacagat agcgcccgtg cagccgggta
    20101 agttgctgat tagcgagatg gactaaatta tccagcgtca gcccctgggc aaacttgcgg
    20161 aatttatcgc cctctttgga acctattagc gaattcagat atccccagtc ctcaacctgc
    20221 tgcgtcattt gagcaatttg ctgcattaag gtttgttgtt gctgacggtt atctgcatcc
    20281 tgcttcagct gctggcgaat ctcgccttga ctcgtggtgt tttcacgcaa cttttggtga
    20341 gtttgcgcta actcttgctg aatctgctcc accgtcacag tgagagccaa cccgtcaggt
    20401 cggtgttgtt gatgctgtgc cagcgtttct gctgtctgag tgaccagcgt ttgcgcctga
    20461 cggcgctggt tttccagatt ctgcttgagc tgttccagct gcgttagtgt ttgttcatcc
    20521 attagcgccg caaggaacgc ctgctgatcg tcaaagacgc tggcctgtag cgcggtgtca
    20581 aactgcgcct gggctttttg cagactttgc gccgccagaa catcctgttg ctgtaacgtc
    20641 tgctgctggc tgtgtaatgc gagacattgt tcatgaacct gccgccagtt atccagcgcc
    20701 acagtttctt cgctgtgcgg gagatcatca ctttgcggca acgtttccag aatcggcgtc
    20761 agctgctgaa tacggttttg cagcgtggtt aattcgttct ggcgttgctg ccagctctgc
    20821 gcttcttgct gacgtgtcgc caaccagctc tcttcttcat cttcctgtgg caatgtcagt
    20881 gcataacccg ccaatgccgt taagagctgc tgctggcgtt gttcaatttg ctgttgatac
    20941 tggataattt gctgattatg cgcggcaatc tgcccttgta attcatgtcg ttggctgagt
    21001 aaccgcagct ggcgtttgtg ctcatcttgt gcatccagcc acggttgaat atcgtcctgt
    21061 ggctgcaagg tgatattgag gctggccgtg acggcttgcc attgttgagt aagtgcttgc
    21121 tcatcttgtc ggaggctttg cgcttcgttt tcatcgcgct gaagctgctt cgttaatgca
    21181 tccagttgcc cgcgcagcgc cgcaccttct tcgccgagct ttttaacttc gttttccagc
    21241 gccagtaatc gagactgatt aacaccaggc tccagcgcct gatacgcctt gaccgccggg
    21301 tggctggtgg aaccacaaag tggacaaggc tgacccgcct gtaactgtgc acgctgggct
    21361 tccagcgttt tgatgcgcgc ttcctgctcg caaatggttt tcacatcggc aagttgctgc
    21421 gtcttttctt tataacgctg gcgcatttcg ttaagtgcga cattacgttg cgtctgttcc
    21481 agagatccat tctggatagt gaccattaac tgcgccagac gtttttgttg gggaacaatc
    21541 tgcccatgca gcgcgaccag gcgctgacgc agtgggcgtt gctcagcatg ttgcgccagg
    21601 gcggtagcaa cttcatcggc ggttaacgtc aacgtgatcg ccgcaagcgc attaagtttt
    21661 tgctcagcat gggttaactg ttgctgccat tgccgcagat gctcgcgatc gctggtttgt
    21721 tgggagaact gcgcacgcca acccgccagt tcgttgttcc actgacggaa gcggtcgtgt
    21781 tcctgtaacc aggtattcag gctttgttgc tgctgctgta attctgctga ctgcttcgcc
    21841 gcgtggtggc gaatgctcgc gcgacgcgcc attgtgctct gtaagcgagt atttacttct
    21901 tcaatctgct ggcgagtatg cgccagcgcc gcgctgtgtt ctgcgatgcg ttcccagtgc
    21961 ggacgaagat ttcgtgccgg ttgtgccaga ctgagcgccg ccagttgagg ttgcgctttt
    22021 tcttcttcgg ctaacgcctg ttgcaaggcc tgctgacggc ggctggcttc ttgctgcaat
    22081 tcgtccagac gcgttaacca gtttagtgat tgttgttctt gctgctgcgc ggtaagtaac
    22141 tgtttttctt cgtcagtaag tacctgcaaa ctcgctgtca gcgattgcac ttgttccggc
    22201 gtgagcaacg cgacgccgct ggcctgcgct tgcagcttct ccagctctgt gcgggccgat
    22261 ttgtgctgct caaaaaccat cgccgagatt tgcccgtaga tttcagtgcc ggttaactcc
    22321 tcgagcaatt ccgcgcgttc tttgggtttg gcattcagga aggcagcaaa ttgcccctgc
    22381 gaaagcagca tcgaacgggt gaagcgcccg taatccagcc cggttaacgt cgctgtcagt
    22441 tccagcttat ctttcacttt gtcggcgaga attttgccgt cggcgcagcg cgccagctct
    22501 acgcgtggca cctgcaaatt accgtcgggt tggttacgcg cccgattctg gctccagaat
    22561 gcacggtacg cttcaccttt cacttcaaac tccacctccg ccagacattc ggcggtatcg
    22621 cgggtcatga gatcattttg cgattgtgaa acgttagaga gacgcggagt ttcgtgatac
    22681 agcgccagac aaatggcgtc cagcagggtg gttttccccg caccggttgg gccggtaatg
    22741 gcaaacagcc cgttgctggc gaacggctcg cgggtgaaat caatcttcca ttcgcctttt
    22801 aatgagttca ggtttttcag gcgcaggctg agaattttca tgcttcgtgt tctccggcga
    22861 gggtatgcaa cgtcgtggtg aaaagatgct gcagacgttg ctgctgcgat tcatccagtt
    22921 cttccagtgc cagacggcga ttgaacacct cttcgacgct gagttcgctg agggtttcac
    22981 gctgttggct ggctaacaca cgctcgcgct gttcacgact ccgacgtacc agcaatactt
    23041 cgacaggcaa tgattcggct aatgcctgga ttttgcgctg aatatcatgc agatactcat
    23101 cagtagtgat ttcgatatcc agccagacag gtggctcctg cgatacatcg cgccactgtt
    23161 ccagctgtgc ggtaatcgac gccagatcgc ctttcagcac cgccatgggt tgagttaccg
    23221 gtacgttcag gttttccacg ctctctaatt tgccgtttga aaatgtcacc agatggacat
    23281 atttactctt accgcattca tcaaaactca gtggaatggg ggaaccgcaa tagcgaacat
    23341 gttccatgcc gccaataatc tgtgcgcggt gaatatgccc gagcgcgatg tagtcggctg
    23401 gtggaaagtt ttgtgccgga aacgcgtcca gcgtgccaat ataaatgtca cgcacggcgt
    23461 cacttttact ggcacccacg gtcgttaaat gtcccgtggc gatgatgggc agaggctgat
    23521 cgccgcgcag tttgcaggca tcggcatagt gttgttggta ataatcggta atcgctgcca
    23581 gtaaatgctg ctgtttttca ataccgttaa gccccgcctg gctggaaata atgtcacgcg
    23641 ggcgtaaaaa cggaatgggg cacagcactg cgcctggcgt cccgtcgcga cgaggcaaga
    23701 tttgcggcgc atgtccggcg ctggcgacca cggttgtatt gaggaacgcc atgatatcgc
    23761 gcgattcatt cagcgtggcg accgagtcat ggtttcctgc cagtaccacc agatgacagc
    23821 cagtttgctg taaattgaca acaaaacggt tgtataacgt gcgggcgtaa ctgggcggcg
    23881 agccggtatc gaaaacatca ccagcaacaa taatcgcatc cacctgatgg gtttgtgctg
    23941 tctccagcag ccagtcaaga aaagcctgat gttcagcttc gcggctttta ctgtagaagt
    24001 tctggccgag atgccagtct gaggtgtgaa ggatgcgcat aacggttccc tggcgaaaaa
    24061 gcatggtcgc gattataccc aaacagatgt gccatttgct tttttctgcg ccacggaaat
    24121 caataacctg aagatatgtg cgacgagctt ttcataaatc tgtcataaat ctgacgcata
    24181 atgacgtcgc attaatgatc gcaacctatt tattacaaca gggcaaatca tggcgagacg
    24241 tattctggtc gtagaagatg aagctccaat tcgcgaaatg gtctgcttcg tgctcgaaca
    24301 aaatggcttt cagccggtcg aagcggaaga ttatgacagt gctgtgaatc aactgaatga
    24361 accctggccg gatttaattc tccttgactg gatgttacct ggcggctccg gtatccagtt
    24421 catcaaacac ctcaagtgcg agtcgatgac ccgggatatt ccagtggtga tgttgaccgc
    24481 cagaggggaa gaagaagatc gcgtgcgcgg ccttgaaacc ggcgcggatg actatatcac
    24541 caagccgttt tcgccgaagg agctggtggc gcgaatcaaa gcggtaatgc gccgtatttc
    24601 gccaatggcg gtggaagagg tgattgagat gcagggatta agtctcgacc cgacatctca
    24661 ccgagtgatg gcgggcgaag agccgctgga gatggggccg acagaattta aactgctgca
    24721 cttttttatg acgcatcctg agcgcgtgta cagccgcgag cagctgttaa accacgtctg
    24781 gggaactaac gtgtatgtgg aagaccgcac ggtcgatgtc cacattcgtc gcctgcgtaa
    24841 agcactggag cccggcgggc atgaccgcat ggtgcagacc gtgcgcggta caggatatcg
    24901 tttttcaacc cgcttttaac gccttgctca tcggacgcag agcagggctt atgatttctt
    24961 aactggagta tcttacgtgc tggaacggct gtcgtggaaa aggctggtgc tggagctgct
    25021 actttgttgc ctcccggctt tcatcctggg tgcatttttt ggttacctgc cctggttttt
    25081 gctggcatcg gtaacaggac tgcttatctg gcatttctgg aatttattgc gcctttcatg
    25141 gtggttgtgg gtggatcgca gtatgacccc gccaccgggg cgtggtagct gggaaccgct
    25201 actatacggc ttacaccaga tgcagctgcg aaataaaaaa cgccgccgtg aactgggcaa
    25261 tctgattaaa cgctttcgta gcggcgcgga gtcgctgccc gacgcggtgg tgctgaccac
    25321 ggaagagggc ggtattttct ggtgtaacgg tctggcgcaa caaattcttg gtttgcgctg
    25381 gccggaagat aacgggcaga acatccttaa cctactgcgt tacccggagt ttacgcaata
    25441 tctgaaaacg cgtgattttt ctcgcccgct caatctggtg ctcaacaccg ggcggcatct
    25501 ggaaattcgc gtcatgcctt atacccacaa acagttgctg atggtggcgc gtgatgtcac
    25561 gcaaatgcat caactggaag gggcgcggcg taactttttt gccaacgtga gccatgagtt
    25621 acgtacgcca ttgaccgtgt tacagggtta cctggagatg atggatgagc agccgctgga
    25681 aggcgcggta cgcgaaaaag cgttgcacac catgcgcgag cagacccagc ggatggaagg
    25741 gctggtgaag cagttgctaa cgctgtcgaa aattgaagcc gcgccgacgc aattgctcaa
    25801 tgaaaaggtt gatgtgccga tgatgctgcg cgttgttgag cgcgaggctc agactctgag
    25861 tcagaaaaaa cagacattta cctttgagat agataacggc ctcaaagtgt ctggcaacga
    25921 agatcagcta cgcagtgcga tttcgaacct ggtctataac gccgtgaatc atactccaga
    25981 aggcacgcat atcaccgtac gctggcagcg agtgccgcac ggtgccgaat ttagcgttga
    26041 agataacgga ccgggcattg caccggagca tattccgcgc ctgaccgagc gtttttatcg
    26101 cgttgataaa gcgcgttccc ggcaaaccgg cggtagcgga ttagggttag cgatcgtgaa
    26161 acatgctgtg aatcatcacg aaagtcgcct gaatattgag agtacagtag gaaaaggaac
    26221 acgtttcagt tttgttatcc cggaacgttt aattgccaaa aacagcgatt aatccgcctt
    26281 tgtcatcttt tattgccata agccagtcga tgctggctta ttttctttgc agtcaaaata
    26341 cgggcgttag attttacaac ggttggtgat tttttgttcg catgattagc catgtctttt
    26401 tcacggaaat agtgttttat actggttggt gatttcttat cgctatatac ctctggtttt
    26461 tagatccctc cttgctttaa aacgttataa gcgtttaaat tgcgcttcag gtgctgtcat
    26521 actgactgca ttaacgcggt aaatcgaaaa actattcttc gccgcgccag gttgggagta
    26581 tttcccgcta aaattgttta aatataccgc tgtatcatcc ccagggattg gcacaaaaat
    26641 ttaacgttac aacaccacat ccacaggcag tatgatttat gacccatcaa ttaagatcgc
    26701 gcgatatcat cgctctgggc tttatgacat ttgcgttgtt cgtcggcgca ggtaacatta
    26761 ttttccctcc aatggtcggc ttgcaggcag gcgaacacgt ctggactgcg gcattcggct
    26821 tcctcattac tgccgttggc ctgccggtat taacggtagt ggcgctggca aaagttggcg
    26881 gcggtgttga cagcctcagc acgccaatcg gtaaagtcgc tggcgtgctg ctggcaacgg
    26941 tttgttactt ggcggtgggg ccgcttttcg ctacgccgcg tacagctacc gtttcctttg
    27001 aagtggggat tgcgccgctg acgggtgatt ccgcgctgcc gctgtttatc tacagcctgg
    27061 tctatttcgc tatcgttatt ctggtttcgc tctatccggg caagctgctg gataccgtgg
    27121 gcaacttcct tgcgccgctg aaaattatcg cgctggtcat cctgtctgtt gccgctattg
    27181 tctggccggc gggttctatc agcacggcga ctgaggctta tcaaaacgct gcgttttcta
    27241 acggcttcgt taacggctat ctgaccatgg atacgctggg cgcaatggtg tttggtatcg
    27301 ttattgttaa cgcggcgcgt tctcgtggcg ttaccgaagc gcgtctgctg acccgttata
    27361 ccgtctgggc tggcctgatg gcgggtgttg gtctgactct gctgtacctg gcgctgttcc
    27421 gtctgggttc agacagcgcg tcgctggtcg atcagtctgc aaacggcgct gctattctgc
    27481 atgcttacgt tcagcacaca tttggcggcg gcggtagctt cctgctggcg gcgttaatct
    27541 tcatcgcctg cctggtaacg gcagttggcc tgacctgtgc ttgtgcagaa ttctttgccc
    27601 agtacgtacc gctctcttat cgtacgctgg tgtttatcct cggcggcttc tcgatggtgg
    27661 tgtctaacct cggcttaagc cagctgatcc agatctccgt accggtgctg accgctattt
    27721 atccgccgtg tatcgcactg gttgtattaa gttttacacg ctcatggtgg cataattcgt
    27781 cccgcgtgat tgccccgccg atgtttatca gcctgctttt tggtattctc gacgggatca
    27841 aagcatctgc attcagcgat atcttaccgt cctgggcgca gcgtttaccg ctggccgaac
    27901 aaggcctggc gtggttaatg ccaacagtgg tgatggtggt tctggccatt atctgggatc
    27961 gcgcggcagg tcgtcaggtg acctccagcg ctcactaaat cactgaacat ttgttttaac
    28021 cacggggctg cgatgccccg tggtttttta ttgtgttgat gggttaggaa ttgatggaaa
    28081 gtaagaacaa gctaaagcgt gggctaagta cccgccacat acgctttatg gcactgggtt
    28141 cagcaattgg caccgggctg ttttacggtt cggcagacgc catcaaaatg gccggtccga
    28201 gcgtgttgtt ggcctatatt atcggtggta tcgcggcgta tatcattatg cgtgcgctgg
    28261 gggaaatgtc ggtacataac ccggccgcca gctctttctc gcgttatgcg caggaaaacc
    28321 tcggcccgct ggcaggttac attaccggct ggacctactg ctttgaaatc ctaattgtcg
    28381 ccatcgccga tgtgaccgct tttggtatct atatgggtgt ctggttcccg acggtgccgc
    28441 actggatttg ggtactgagc gtggtgctga tcatttgcgc cgtaaacctg atgagcgtga
    28501 aggtattcgg tgagctggaa ttctggttct cgttctttaa agtcgccacc atcatcatca
    28561 tgattgtcgc cggtttcggc atcatcatct gggggattgg caacggcggg caaccgaccg
    28621 gtattcataa cctgtggagc aacggcggct tcttcagtaa cggctggctt ggcatggtaa
    28681 tgtcgttgca aatggtgatg tttgcttacg gtgggatcga aattatcggg attaccgccg
    28741 gtgaagcgaa agatcctgag aaatcgattc cgcgtgcgat taactccgtg ccgatgcgta
    28801 ttctggtgtt ctacgtcggt acgctgttcg tcattatgtc tatctacccg tggaatcagg
    28861 ttggcactgc cggtagcccg ttcgtgctga cgttccagca tatgggcatt acctttgccg
    28921 ccagcattct taactttgtt gtgctgaccg cttcgctgtc ggcaattaac agtgacgtat
    28981 ttggcgtagg ccgtatgctc cacggtatgg cagagcaggg cagcgcaccg aaaattttca
    29041 gcaaaacgtc gcgtcgcggt attccgtggg ttacggtgct ggtgatgact accgcgctgc
    29101 tgtttgcggt gtatctgaac tacatcatgc cggaaaacgt cttcctggtg atcgcttcgc
    29161 tggcaacctt cgccacggta tgggtgtgga ttatgatcct gctgtcgcaa atcgccttcc
    29221 gtcgccgttt gccgccagaa gaagttaagg cgctgaaatt taaagtgccg ggtggggtag
    29281 caacgaccat cggcgggctg attttcctgc tctttattat cgggttgatt ggttatcacc
    29341 cggatacgcg tatctcgctg tatgtcggtt tcgcgtggat tgttgtgctg ttgattggct
    29401 ggatgtttaa gcgtcgccac gatcgtcagc tggctgaaaa ccagtaatcc ttgcgttctg
    29461 taagccggtt caggaggaaa tcctgatccg gctattccga aagttatccg cccccgtcct
    29521 cctcccccaa atatccttca gatgatgagt gatcctgcat taggctatgg caaggtgatc
    29581 agattttcat cacaggggaa ttatgatgtt aaatgcatgg cacctgccgg tgcccccatt
    29641 tgttaaacaa agcaaagatc aactgctcat tacactgtgg ctgacgggcg aagacccacc
    29701 gcagcgcatt atgctgcgta cagaacacga taacgaagaa atgtcagtac caatgcataa
    29761 gcagcgcagt cagccgcagc caggcgtcac cgcagggctt cagccgaatg ccgccggcac
    29821 gactggagca gtttgccgtc gatgtaccgg atatcggccc acaatgggct gcggatcaga
    29881 ttttttatca gatcttccct gatcgttttg cgcgtagtct tcctcgtgaa gctgaacagg
    29941 atcatgtcta ttaccatcat gcagccggac aagagatcat cttgcgtgac tgggatgaac
    30001 cggtcacggc gcaggcgggc ggatcaacgt tctatggcgg cgatctggac gggataagcg
    30061 aaaaactgcc gtatctgaaa aagcttggcg tgacagcgct gtatctcaat ccggtgttta
    30121 aagctcccag cgtacatcaa tacgataccg aggattatcg ccatgtcgat ccgcagtttg
    30181 gcggtgatgg ggcgttgctg cgtttgcgac acaatacgca gcagctggga atgcggctgg
    30241 tgctggacgg cgtgtttaac cacagtggcg attcccatgc ctggtttgac aggcacaatc
    30301 gtggcacggg gggagcttgt tacaaccctg aatcgccctg gcgcgactgg tactcgttta
    30361 gtgatgatgg cacggcgctc gactggcttg gctatgccag cttgccgaag ctggattatc
    30421 agtcggaaag tctggtgaat gaaatttatc gcggggaaga cagtattgtc cgccattggc
    30481 tgacagcgcc gtggagtatg gacggctggc ggctggatgt ggtgcatatg ctgggggagg
    30541 cgggtggggc gcgcaataat atgcagcacg ttgctgggat caccgaagcg gcgaaagaaa
    30601 cccagccgga agcgtatatt gtcggcgaac attttggcga tgcacggcaa tggttacagg
    30661 ccgatgtgga agatgccgcc atgaactatc gtggcttcac attcccgttg tggggatttc
    30721 ttgccaatac cgatatctct tacgatccgc agcaaattga tgcccaaacc tgtatggcct
    30781 ggatggataa ttaccgcgca gggctttctc atcaacaaca attacgtatg tttaatcagc
    30841 tcgacagcca cgatactgcg cgatttaaaa cgctgctcgg tcgggatatt gcgcgcctgc
    30901 cgctggcggt ggtctggctg ttcacctggc ctggtgtacc gtgcatttat tacggtgatg
    30961 aagtgggact ggatggtaaa aacgatccgt tttgccgtaa accgttcccc tggcaggtgg
    31021 aaaagcagga tacggcgtta ttcgcgctgt accggcgaat gattgtgctg cgtaagaaaa
    31081 gtcaggcgct gcgtcgtggc ggctgtcagg tgctgtatgc ggaagataac gtagtggtat
    31141 ttgtccgcgt gctgaatcag caacgtgtac tggtggcaat caaccgtggc gaggcctgtg
    31201 aagtggtgct acccgcgtca ccgtttctca atgccgtgca atggcaatgc aaagaagggc
    31261 atgggcaact gactgacggg attctggctt tgcctgccat ttcggctacg gtatggatga
    31321 actaacgttt tataacgcct tgtgtgacgc ctgcgccatc atccgcggat aaaactgcca
    31381 gaagcgggtt ccgagggcgg tataatgggc gtttaaatcg taccaggagt cacgcagggc
    31441 atccagacgt gggcgacggc ttgccatgcc gtttaacacg ttctggatga aatccatatc
    31501 gcgatagcgc accagccact gctctgacca caagtaattg tttagattga taaaacgtgg
    31561 cggtgagtcc ggcaaaatcg tcatcacttg cttgcgggca taacagataa attcctgtag
    31621 cggaaagtcc ggcgacagct gcgaccagtg gcgggaaaga aagtgatccc acatgacatc
    31681 cagcgtaata ggcgcaacgc ggcgcgtttc acgacgaaac cactcccgtg cttcgcggac
    31741 ttccggcaga ttgtcagtca atacgtcgat acgtcgatgc atatgaatgc cagccacgac
    31801 gtcgggcgga aaactttctt caggatttcc gcgtacgaaa tcagccagta aattgccgga
    31861 aagcgagctt tccgcgagat gggctaaatg caggtgagct aaaaaattca tcgattatat
    31921 tctatccaaa ggggggtaaa ggttgcaggg agagcgcccc ggcactagac tacccgcctc
    31981 ttattttagt ctgagtcagt gtcatgcgcg ttaccgattt cttctttgaa ttgcccgaat
    32041 ccctgattgc ccactacccc atgcctgaac gcagtagctg tcgtttactg tcgctggacg
    32101 ggcctacggg cgcgctgacg cacggtactt tcaccgattt actcgataag ctcaaccccg
    32161 gcgatcttct ggtttttaat aatactcgcg tgatcccggc gcgcctgttt gggcgtaaag
    32221 ccagcggcgg caagattgaa gtgctggttg agcggatgct cgacgacaaa cgcattcttg
    32281 cgcatattcg cgcctcgaaa gcgccaaaac ctggcgcaga actgctgctg ggcgatgacg
    32341 aaagtattaa cgcaacaatg accgcgcgcc acggcgcact gtttgaagtc gaattcaatg
    32401 atgatcgttc ggtactggat atcctcaaca gtatcgggca tatgccgctg ccgccgtata
    32461 tcgaccgtcc ggacgaagac gctgaccgcg aactttatca aaccgtttat agcgaaaaac
    32521 cgggcgcggt tgcagccccg accgctggtc tgcattttga cgagcctttg ctggaaaaat
    32581 tgcgcgccaa aggcgtggag atggcgtttg tgacgttgca cgttggtgcg ggcaccttcc
    32641 agccggtgcg cgtcgacacc attgaagatc acatcatgca ctcggaatac gctgaagtac
    32701 cgcaggatgt ggtagacgcg gtactggcgg cgaaagcgcg cggtaaccgg gtgattgcgg
    32761 ttggcaccac ttcagtacgt tcgctggaaa gcgcggctca ggcagcgaaa aacgatctca
    32821 ttgaaccgtt cttcgacgat acccaaatct ttatctatcc gggcttccag tacaaagtgg
    32881 tcgatgcgct ggtgacgaac ttccacttgc cagagtcgac gctgattatg ctggtttcgg
    32941 cctttgccgg ttatcaacac accatgaacg cctataaagc agcggtagaa gagaaatatc
    33001 gcttttttag ttacggtgat gcgatgttta ttacgtacaa tccgcaggca attaatgagc
    33061 gcgtcgggga gtaattccgc ggcgctggtt taaaacgttg gactgttttt ctgacgtagt
    33121 ggagaaaaaa tgaaatttga actggacacc accgacggtc gcgcacgccg tggccgcctg
    33181 gtctttgatc gtggcgtagt ggaaacgcct tgttttatgc ctgttggcac ctacggcacc
    33241 gtaaaaggga tgacgccgga agaagttgaa gccactggcg cgcaaattat cctcggcaac
    33301 accttccacc tgtggctgcg cccgggccag gaaatcatga aactgcacgg cgatctgcac
    33361 gattttatgc agtggaaggg gccgatcctc accgactccg ggggcttcca ggtcttcagc
    33421 cttggcgata ttcgtaaaat caccgaacag ggcgtgcact tccgtaaccc gatcaacggc
    33481 gatccgattt tcctcgatcc tgaaaaatca atggagattc agtacgatct tggttcggat
    33541 atcgtcatga tctttgatga gtgtacgccg tatcctgctg actgggatta cgcaaaacgc
    33601 tccatggaga tgtctctgcg ttgggcgaag cgtagccgtg agcgttttga cagtctcgga
    33661 aacaaaaatg cgctgtttgg tatcattcag ggcagcgttt acgaagattt acgtgatatt
    33721 tctgttaaag gtctggtaga tatcggtttt gatggctacg ctgtcggcgg tctggctgtg
    33781 ggtgagccga aagcagatat gcaccgcatt ctggagcatg tatgcccgca aattccggca
    33841 gacaaaccgc gttacctgat gggcgttggt aaaccagaag acctggttga aggcgtacgt
    33901 cgtggtatcg atatgtttga ctgcgtaatg ccaacccgca acgcccgaaa tggtcatttg
    33961 ttcgtgaccg atggcgtggt gaaaatccgc aatgcgaagt ataagagcga tactggccca
    34021 ctcgatcctg agtgtgattg ctacacctgt cgcaattatt cacgcgctta cttgcatcat
    34081 cttgaccgtt gcaacgaaat attaggcgcg cgactcaaca ccattcataa ccttcgttac
    34141 taccagcgtt tgatggcggg tttacgcaag gctattgaag agggtaaatt agagagcttc
    34201 gtaactgatt tttaccagcg tcaggggcga gaagtaccac ctttgaacgt tgattaatat
    34261 taataatgag ggaaatttaa tgagcttttt tatttctgat gcggtagcgg caacgggtgc
    34321 accggcgcaa ggtagcccga tgtctttgat tttgatgctg gtggtattcg gtctgatttt
    34381 ctatttcatg atcctgcgtc cacagcagaa gcgcaccaaa gaacacaaaa agctgatgga
    34441 ctccattgcc aaaggtgatg aagttctgac gaacggtggc ctggttggtc gcgtaaccaa
    34501 agtagcggaa aacggctaca ttgctatcgc gctgaatgac accactgaag tagttattaa
    34561 acgtgacttc gtagctgccg tcctgccgaa aggcaccatg aaggcgctgt aattaaaatt
    34621 tttccctaag ggaattgccg tgttaaaccg ttatcctttg tggaagtacg tcatgctgat
    34681 cgtggtgatt gtcatcggtc tgctgtatgc gcttcccaac ctgtttggtg aggatccggc
    34741 tgttcagatc actggtgcgc gcggagtcgc cgccagtgag caaacgctga tccaggtcca
    34801 gaaaacgtta caagaagaaa aaataactgc taagtctgtg gcactggaag agggcgctat
    34861 tcttgcgcgc ttcgactcca ctgacaccca gctgcgcgct cgtgaagcat taatgggcgt
    34921 tatgggtgac aaatacgtcg tggcgcttaa ccttgccccg gcaacgccgc gctggctggc
    34981 agctattcac gctgagccga tgaagctcgg ccttgacctg cgtggcggcg ttcacttcct
    35041 gatggaagtg gatatggaca ccgcgcttgg caaactccag gaacaaaata tcgatagcct
    35101 gcgcagcgat ctgcgtgaaa agggcatccc gtataccacc gttcgtaaag aaaacaacta
    35161 cggcctgagc attactttcc gcgatgctaa agctcgtgat gaagccattg cgtatctgag
    35221 caagcgccat ccggacctgg tgattagtag ccagggcagc aaccagctgc gtgcggtaat
    35281 gagcgatgct cgtctgagtg aagcgcgtga atatgcggtg cagcagaaca tcaatatcct
    35341 gcgtaaccgt gtaaaccaac ttggcgtagc ggagccggtg gttcagcgtc agggtgctga
    35401 ccgtatcgtt gttgaactgc caggtattca ggacactgcg cgtgcgaaag agattctggg
    35461 tgcgacggca acgctggaat tccgtctggt aaacaccaac gttgaccagg ccgctgcggc
    35521 atccggtcgc gtaccgggcg actctgaagt gaaacagacc cgcgaaggtc agccagttgt
    35581 gctgtacaaa cgcgtaattc tgaccggtga ccatatcacc gactccactt ccagccagga
    35641 cgaatacaac caaccgcagg ttaacatctc gctcgatagc gctggtggta acatcatgtc
    35701 taacttcact aaggacaaca tcggcaaacc gatggcaacc ctgtttgtgg agtacaaaga
    35761 cagcggtaag aaagatgcaa atggtcgtgc ggttctggtg aaacaggaag aggtgattaa
    35821 catcgccaac atccagtctc gtctgggtaa cagcttccgt atcaccggca tcaacaaccc
    35881 gaacgaagcc cgtcagctgt cactgctgct gcgtgccggt gcgttgatcg cgccgattca
    35941 gattgttgaa gaacgtacca ttggcccaac cctgggtatg cagaacattg aacaggggct
    36001 ggaggcttgc cttgccggtc tgctggtttc tattctgttc atgatcatct tctataagaa
    36061 gtttggtctg attgcgacca gtgctctgat tgccaacttg atcttaatcg tcggcattat
    36121 gtcgctgttg ccaggcgcaa cgctgagtat gccaggtatc gcgggtatcg tcttaaccct
    36181 tgcggtggcg gtcgatgcga acgtactgat caacgaacgt attaaagaag agttgagcaa
    36241 cggacgtact gttcaacagg caattgatga aggttatcgt ggcgcattca gttctatctt
    36301 cgatgcgaac atcaccacgc tgattaaagt catcatcctg tacgcagtgg gtaccggggc
    36361 aattaaaggg ttcgcgatta ctaccggtat cggtgtggcg acgtcgatgt ttaccgcgat
    36421 tgtcggtacg cgtgccatcg taaacctgct atatggcggc aagcgcgtca agaagctgtc
    36481 aatctgagga gtgcgatgtg gcacaggaat atactgttga acaactaaac cacggccgta
    36541 aagtctatga ctttatgcgc tgggactact gggctttcgg catctctggt ctgctgttaa
    36601 tcgccgctat cgttattatg ggcgtgcgcg gctttaactg ggggctggat ttcaccggtg
    36661 gtacggttat cgaaattacg ctcgaaaaac cggctgaaat tgacgtaatg cgtgatgcat
    36721 tgcaaaaagc cggttttgaa gagccaatgc tgcaaaactt tggtagcagc cacgacatca
    36781 tggtccgtat gccgcctgct gaaggcgaaa ccggcggtca ggtgctgggc agccaggttc
    36841 tgaaggtgat taacgaatcc accaatcaga atgcagcagt gaagcgtatt gagttcgtcg
    36901 gtccgagcgt gggggcagac cttacgcaaa ccggtgcgat ggcgttgatg gcggcgctgc
    36961 tgtctatcct tgtgtacgta ggttttcgct ttgagtggcg actggcggca ggggttgtta
    37021 ttgcgctggc gcacgacgtt atcattacgc tgggtatttt gtcgttattc catatcgaga
    37081 ttgacctgac cattgtggca tcgttgatgt cggttatcgg ttactcgctt aacgacagta
    37141 tcgtggtatc ggaccgtatt cgtgaaaact tccgcaagat ccgtcgcggt acgccttacg
    37201 aaatctttaa cgtgtccttg acccagacgc tgcaccgtac cttgatcaca tccggtacta
    37261 ccttgatggt tatcctgatg ctgtacctct tcggtggtcc ggtactggaa ggcttctcgc
    37321 tgaccatgct tatcggtgtt tccatcggta ctgcatcttc catctatgtg gcatctgcgt
    37381 tggctctgaa actgggtatg aagcgcgaac acatgttgca gcagaaagtg gaaaaagaag
    37441 gggcggatca gccgtcaatt ctgccgtaat caagttcccg ttgatgttga aaatcccggt
    37501 cagaagatcg ggattttttt tgatgtatgg agattgcgaa aatccccgca tcttgggaaa
    37561 ctgcgcgtaa ccctacattt catccaggta actcttcatg gctatcatcc caaaaaacta
    37621 tgcgcggtta gaaagcggct atcgcgaaaa agcattaaaa atatatccgt gggtctgcgg
    37681 tcgctgttcc cgcgagtttg tttattccaa cctgcgtgaa cttaccgttc accacattga
    37741 tcacgaccat accaataacc cggaagatgg cagtaactgg gaattgcttt gtctctattg
    37801 ccacgatcat gagcattcga aatataccga agcggatcag tatggtacga ccgttatcgc
    37861 aggggaagat gcgcagaaag atgtcggtga agcgaagtac aacccattcg ctgacctgaa
    37921 agcgatgatg aacaagaaga agtgattaaa acgtaaaatt gcctgatgcg ctacgcttat
    37981 caggcctacg ttatttcagc aatatattga atttacgtgc ttttgtgggc cggacaaagc
    38041 gtttacgccg catccggcat gaacaaagct cacgttgtta acaatcagaa atgccgggaa
    38101 taaatcccgg cattttcata atcagaagtt gtaacctact accaggtaac caccccagcc
    38161 ggtagagcga acgttgaagt tgccgttgcc gaagttcagt tctgcatcgt cgttccactg
    38221 accaccgtcg tgccagtaac gagctacgac agagtagtgc cagtgatcgt agttcagagc
    38281 cagaatatgg ctggaagcga tagagttatt agtacgggtc ttaataccgt tgattgcgtt
    38341 accgctgtca tcccctaaat cggaacccca gtcgaagttg gtgaagccga tgtagctcag
    38401 ctgaccgccc cacagatcgg taatcggcac aaagtattta attttgaaac ggtaaccgtc
    38461 ccactcgttt tcgttcgctg cgccatagtt ctgccactgg tatttcgcat agacgttcat
    38521 ggacaggctc atcggcagac cagtatcgat gtcggtaccc agacccatgt accaggtgct
    38581 ctggcgacca tttttattac gacccatgtc gtaaatgtag ttgttcgcga agtaccactc
    38641 tttgaacgga ccgaagttaa ggtcagtatt ggtcagcttg tcgatggaga aacgtggttc
    38701 gatttccata aacagcggag aaccgtggtt ccagatacct ttagcatcgg agttaccgcc
    38761 gaagaatacc ggcgcatccg cataaccata gaagtcgaac cagtcttttt tagcgaatgc
    38821 ttcgtactca aggtaggtat cgttgcggat ctgcggtccg aaacgggtgt gatagcttcc
    38881 gacaacgtta acgctctggt gccaccagtc ggaaagatac tgcggtttgt cgttttcagc
    38941 tgcgttgaca gtaaaagacg aagagagcgc cagtaccgca ccggctgcca gtaatgtttt
    39001 tttcatatgt atgccactgt ttgaaaatcc cttgcgggag tgaaaaaggc gcaaattgcg
    39061 tttctaaata tttcgtatac agaggagcct attataaaaa tcattgctca caaaaatatg
    39121 tttcgtttca cagttctatc atttacgtaa tcgattgcgt tcacgtttac acacattcgg
    39181 gcggggattg tactgacttt cgcccttgtt gcaaactttt actatcaatc caggtgatgt
    39241 aagcagagta acaaaatgac ggggaaagat gttgcgggac gcacaaaatc gttccgaaat
    39301 acgtttactg cataacaggt tgaatgtcgt ggatacgtac aaagcctaac tgatccacag
    39361 aaatcccctt taattgtaac ggaatatcaa catcgctggg ggcgaggacg ctggcagggg
    39421 cattaatcaa ttgattctgc acattgattt cctgaaagtt gtctattgtt ccctgaattt
    39481 gcccatattc aacagttccg ctgaatgcgg gcaagggatc attcgactca ccctgaatac
    39541 gtagtgtaag ggtggtgcca tctgtatcag gcgtaatatt caccagcgac atacgtaaag
    39601 taccgatctg gctttccagt cttgccggtg ttttcgctcc aggcagaagg taacccccac
    39661 tgctggattt tgcattcagc ctgttttgct gagtgatttt gactgtttct tgattgagtt
    39721 gcgtcatctc ctggttcagc gtgctgacgc tgtgtttcat ctgtcgcact tcactttgct
    39781 gcacacaggc gctaaggctg aacaggcttc ccagcaggag cagtcgaaaa acgttagtat
    39841 tcataggtcg tccctttctc gtcgcaatta gcctaatggt agagggtaaa agcagcgaaa
    39901 gcatcgatcc tttatctcaa aagcgttgcg cctttgttgt atcgtcagtt cagggtaaaa
    39961 tagatttccg ttaaccacct ggtcaggacg ccgtatgcat tgcccattct gtttcgccgt
    40021 ggacactaag gtaattgact ctcgtctcgt gggcgagggt tcatccgtac gccgccgtcg
    40081 gcagtgtctg gtgtgtaatg aacgtttcac cacctttgaa gtggcggagc tggttatgcc
    40141 gcgtgttgta aaaagcaacg acgtgcgtga accgtttaat gaagagaaat tgcgtagcgg
    40201 aatgctgcgg gcgctggaaa aacgtccggt gagttccgat gacgttgaaa tggcaatcaa
    40261 tcatattaaa tcgcagctgc gcgccaccgg tgagcgcgaa gtaccgagca agatgattgg
    40321 caatctggtg atggagcaat tgaaaaagct cgataaagtc gcctatatcc gttttgcctc
    40381 tgtctaccgc agtttcgaag atatcaaaga atttggcgaa gagatcgcgc gcctggagga
    40441 ctaagccgtg caggacgagt attatatggc gcgggcgcta aagctggcgc aacgaggacg
    40501 ttttaccacc caccccaacc caaatgtcgg gtgcgtcatt gtcaaagatg gcgaaattgt
    40561 cggtgaaggt taccatcaac gagcgggtga accacatgcc gaagtacacg cgttgcgtat
    40621 ggcgggtgaa aaagccaaag gtgcaacggc gtatgtgaca ctcgaacctt gcagccatca
    40681 cggccgtacg ccgccttgct gtgatgcttt aattgccgca ggtgtggcgc gcgtggttgc
    40741 ctcgatgcaa gatcctaacc cgcaggtcgc tgggcgtgga ctttaccgtc tgcaacaggc
    40801 tggcattgac gtcagccacg gcctgatgat gagtgaagcc gagcaattga ataaaggctt
    40861 tctcaagcgg atgcgcaccg gctttcctta tattcagtta aaacttggcg catcgcttga
    40921 tggtcgcacg gcgatggcga gcggcgaaag ccagtggatc acttcgcccc aggcgcggcg
    40981 tgatgtacaa cgactgcgcg cgcaaagtca tgccatttta accagcagcg ccacggtgct
    41041 ggcggatgat cctgccttaa cggtgcgttg gtccgaactg gatgaacaaa ctcaggcgct
    41101 ctacccgcaa caaaatctcc gccagccggt acgtattgtg attgatagcc aaaatcgcgt
    41161 gacgccggaa catcacattg tgcagcagcc cggcgaaacc tggttcgcgc gtacgcagga
    41221 agattctcgt gagtggccgg aaacggtgcg taccttgctg attccagagc ataaaggtca
    41281 tctggatctg gttgtactga tgatgcaact gggtaaacag cagattaaca gcatctgggg
    41341 ggaagcgggg ccaacgctcg ctggcgcatt gctgcaggcg ggtttagtcg atgagctgat
    41401 tgtctatatc gcacctaaac tattaggcag cgacgcccgt ggattatgca ccctgccagg
    41461 gcttgagaaa ttagccgacg ccccccaatt taaattcaaa gagatacgtc atgtaggccc
    41521 ggatgtttgc ctgcatttag tgggtgcatg atctcccggc tcgaaaggga agcagcgcac
    41581 gaaatattat gctaaaatcc gcccccctgc ggggccatac tcgaacccga aggaagaaaa
    41641 tgaacattat tgaagctaac gttgctaccc cggacgctcg cgtcgccatc accatcgcgc
    41701 gtttcaacaa ctttatcaat gacagcctgc tggaaggtgc aattgacgca ctgaaacgta
    41761 tcggtcaggt aaaagatgaa aacattaccg ttgtttgggt gcctggtgcc tatgagctgc
    41821 cgctggcggc gggtgcactg gctaaaaccg gtaaatacga cgcggtgatt gcgctgggta
    41881 cggttattcg tggtggcact gcccattttg aatatgtcgc tggtggtgca agcaacggcc
    41941 tggcgcatgt tgcccaggac agcgaaattc cggttgcttt tggggttctg accactgaaa
    42001 gcattgaaca agcgatcgaa cgtgctggca ccaaagctgg caacaaaggt gcagaagctg
    42061 cactgaccgc gcttgaaatg attaatgtat tgaaagccat caaggcctga aattagtaag
    42121 gggaaatccg tgaaacctgc tgctcgtcgc cgcgctcgtg agtgtgccgt ccaggcgctc
    42181 tactcctggc agttgtccca gaacgacatc gctgatgttg aataccagtt cctggctgaa
    42241 caggatgtaa aagacgttga cgtcctgtac ttccgtgagc tgctggccgg ggtggcgact
    42301 aataccgcat acctcgacgg actgatgaag ccatacctgt cccgtctgct ggaagaactg
    42361 ggacaggtag aaaaagcagt actgcgcatt gcgctgtacg aactgtctaa acgtagcgat
    42421 gtgccataca aagtggccat taacgaagcg atcgaactgg cgaaatcgtt cggcgcagaa
    42481 gacagccata agttcgtcaa cggtgtactc gataaagcag cacctgtgat tcgccctaac
    42541 aaaaagtaat atccaggccg gtagattcac ggaagaccgt tccatgatcg ccggcctttt
    42601 cttttttacc tgctgaggca taacgtatgg catgtggcga gttctccctg attgcccgtt
    42661 attttgaccg tgtaagaagt tctcgtcttg atgtcgaact gggcatcggc gacgattgcg
    42721 cacttctcaa tatccccgag aaacagaccc tggcgatcag cactgatacg ctggtggcgg
    42781 gcaaccactt cctccctgat atcgatcctg ctgatctggc gtataaagca ctggcggtga
    42841 acctaagcga tctggcagcg atgggggccg atccggcctg gctgacgctg gcattaacct
    42901 tgccggacgt agacgaagcg tggcttgagt ccttcagcga cagtttgttt gatcttctca
    42961 attattacga tatgcaactc attggcggcg ataccacgcg tgggccatta tcaatgacgt
    43021 tgggtatcca cggctttgtt ccgatgggac gagccttaac gcgctctggg gcgaaaccgg
    43081 gtgactggat ctatgtgacc ggtacaccgg gcgatagcgc cgccgggctg gcgattttgc
    43141 aaaaccgttt gcaggttgcc gatgctaaag atgcggacta cttgatcaaa cgtcatctcc
    43201 gtccatcgcc gcgtatttta caggggcagg cactgcgcga tctggcaaat tcagctatcg
    43261 atctctctga cggtctgatt tccgatctag ggcatatcgt gaaagccagc gactgcggcg
    43321 tacgtattga cctggcattg ctgccgtttt ctgatgcgct ttctcgccat gttgaaccgg
    43381 aacaggcgct gcgctgggcg ctctctggcg gtgaagatta cgagttgtgt ttcacggtgc
    43441 cggaactgaa ccgtggagcg ctggatgtgg ctctcggaca cctgggcgta ccgtttacct
    43501 gtatcgggca aatgaccgcc gatatcgaag ggctttgttt tattcgtgac ggtgaacctg
    43561 ttacgttaga ctggaaagga tatgaccatt ttgccacgcc ataaagatgt cgcgaaaagt
    43621 cgcctgaaga tgagtaatcc gtggcatcta cttgctgtcg gattcggaag tggattaagc
    43681 ccgatcgttc ctgggacgat gggctcgctg gcagcgattc cgttctggta tctgatgacc
    43741 tttttgccct ggcagctcta ctcgctggtg gtgatgctgg ggatctgtat cggcgtctat
    43801 ctttgtcatc agaccgcaaa agatatgggc gtgcacgatc acggcagcat tgtctgggac
    43861 gaatttattg gtatgtggat cacgctcatg gcgctgccga ccaatgactg gcagtgggtt
    43921 gccgccgggt ttgtgatttt ccgtattctg gatatgtgga aaccgtggcc gatccgctgg
    43981 tttgatcgca atgtgcatgg cggcatgggg atcatgatag atgatattgt cgccggggtg
    44041 atctccgctg gcatcctgta ctttattggt catcactggc cgctgggcat tctgtcgtag
    44101 ttgtgcaccg atgcctgatg tgacgcttgt tgcgtctcat caggcctgga ctcttattta
    44161 aatcctacta ccggatgcgg tttatacggc gtttccagtt cggcaatctg ttccggcttc
    44221 aaagtgatat ccaccgcgtt caatagctca tcaagctgtt cttcccgcga tgtaccgata
    44281 atcggtgcgg caatgcccgg tttactcaac aaccaagcca gcgcaacttg tgctcgtgtt
    44341 gcaccgagtt cttcactgac gcccgttaac cgctctgcga tctgcgcgtc attttcatcg
    44401 ctttctttat agagattttt ccctacctca tcagacacca gtcgtgcggt agtttctccc
    44461 cacggacgcg tcagtcgccc ccgcgccagc gggctccacg gaatcaccgc cacgccctcc
    44521 tgataacaca gtggcagcat ctcgcgctct tcttcgcgat aaatcagatt gtagtgatcc
    44581 tgcatactga caaactgcgc ccagccgtgc tgtttttgga gttccagtgc ctgagcaaac
    44641 tgcgaagcgt gcattgatga cgcgccgata taacgcgctt tcccggcttt taccacgtcg
    44701 ttcagggctt ccagcgtctc ttcgatcggc gtgttgtaat cccagcgatg aatttgcagg
    44761 atatcgacat aatccatgcc gagacgtcgc aggctgtcgt cgatagagcg caaaatttgt
    44821 gcacgggata atccttccgg taaatcacca acgcgatgga acactttggt ggcaacgacc
    44881 acgtcttcac gacgggcgaa atcccgcagt gcgcaaccaa cgatctcttc gctgctacca
    44941 tcggaatagc tattggcggt atcaaagaaa tttatgccgc cttccagtgc gcgtttaatt
    45001 atgggacggc tgctctcttc cggcagtgtc catgcgtgat taccacgatc tggctcgcca
    45061 aaggtcatac agccgaggca aagtcgggaa acgcgaaggt cggtttttcc taaggggttg
    45121 tattgcatgc tgccactcct gccatgaata atttcttaag catagcagga gtggtgtagg
    45181 gattatgcca gccaggcctt gattttggct tccataccgg cggcatcgag gccgagttcg
    45241 gcgcgcattt cttcctgcgt tccttgcgga ataaagaagt caggcaggcc aatgttcagc
    45301 acggggactg gtttacgatg ggccattagc acttcgttca ctccgctgcc tgcaccgccc
    45361 ataatggcgt tttcttctac ggtaaccagt gcttcatgac tggcggccat ttccagaatt
    45421 aacgcttcat caagcggttt cacaaaacgc atatcgacca gcgtggcgtt cagcgattcg
    45481 gcgactttcg ccgcttctgg catcagcgta ccaaagttaa ggatcgccag tttctcgcca
    45541 cgacgcttca caatgccttt gccaattggt agtttttcca gcggcgtcag ttccacgccg
    45601 accgcgttgc cacgcgggta gcgcaccgct gacgggccat cgttatagtg atagccggta
    45661 tagagcatct ggcgacattc gttttcatcg ctcggggtca taatgaccat ttccggtatg
    45721 cagcgcaggt aagagagatc aaaagcaccc tgatgggttt gaccgtcagc accaacaatg
    45781 cccgcgcggt cgatggcgaa caggaccgga agcttttgaa ttgccacgtc atgcagcacc
    45841 tgatcatagg cgcgttgcag gaaagtggag tagatcgcga caatgggttt gtacccacca
    45901 atcgccagac cggcggcaaa ggtcactgcg tgttgctcgg caattgccac gtcgaagtag
    45961 cgatccggga atttacgaga aaactcgacc atgccggaac cttcacgcat cgccggagta
    46021 atcgccatca gcttgttatc tttcgccgcc gtttcgcaca accagtcgcc aaagattttt
    46081 gaatagctcg gtaaaccgcc gctacttttc ggcaaacaac cgctggaggg atcaaattta
    46141 ggcacggcgt ggaaagtgat tgggtctttt tctgccggtt cataaccacg accttttttg
    46201 gtcatgatat gcaggaactg cgggcctttc aggtcacgca tgttcttcag cgtggtgata
    46261 agccccagca catcgtgacc gtcaaccggg ccgatgtagt taaagcccag ctcttcaaac
    46321 aacgtgccag ggactaccat gcctttaata tgttcttcgg tacgtttgag cagctcttta
    46381 atgggtggca cgccagagaa aacttttttc ccgccttcgc gcagtgaaga gtaaagctta
    46441 ccggaaagca gctgcgccag atggttgttg agcgcgccga cattttcgga aatcgacatt
    46501 tcgttgtcgt tgaggaccac cagcatatca ggacggatat cgcccgcgtg attcatcgct
    46561 tcaaacgcca tgcctgcggt aatcgcgcca tcgccgatta cacagacggt acggcgattt
    46621 ttgccttctt tctcggcagc aaccgcaata ccaattccgg cactgatgga ggttgatgaa
    46681 tgcccgacgc ttaatacgtc atattcgctt tcgccgcgcc acgggaacgg gtgcaggccg
    46741 cctttctgac ggatggtgcc gattttgtcg cggcgtccgg tcaaaatttt atgcggataa
    46801 gcctgatgcc ccacatccca aatcaattgg tcaaacgggg tgttgtagac atagtgcagc
    46861 gccacggtca gttcgaccgt gcccagcccg gaggcgaagt gcccgctgga acggctcacg
    46921 ctgtcgagta aatagcggcg cagttcgtcg cagagtttcg gtaaactctc tttcggcaac
    46981 agtcgtaact cctgggtgga gtcgaccagt gccagggtcg ggtatttggc aatatcaaaa
    47041 ctcatcaggg gcctattaat acttattgtt tatttattac gctggatgat gtagtccgct
    47101 agcgcttcca gtgccgaggt atcgggtgac tgttcagcca gttgtttcag cgactgacgg
    47161 gcatcgtcga tcagatcccg ggctttcttc cgggcttgct caagacccag aagtgcaggg
    47221 taggtacttt taccaagttg ctggtcggca ccctggcgtt ttcccaacgt tgcagtatct
    47281 cctaccacat ccaggatgtc atcctgaacc tggaaggcaa ggccgatgct ctctgcgtac
    47341 ttgtcgagta ctggcagagc acgacgccct ttatctccgg cgcttaatgc accaaggcga
    47401 acggcggcgc gaatcaatgc gccggtttta tgacgatgaa tacgctcaag cgcgtccaga
    47461 ggtacgtgtt tgccttccgc gtctaaatct aatgcctgac caccgcacat tccggcaata
    47521 ccgctggcgc tcgccagttc agaaatcatc gaaattctgt cgcgatccga cacttccggc
    47581 atatcagcat cgcttaaaat cgagaacgcc agcgtttgta aagcgtcgcc agcgagaatc
    47641 gcgtttgctt cgccaaactt cacatggcag gtcggcaaac cgcgacgcag atcgtcatca
    47701 tccatcgccg gtaaatcatc atgaattaat gagtaagcgt ggatacactc tacggcggca
    47761 gcgggtgcgt ccagcgtgtt tgtgctaacg ccaaacatat gaccggtggc ataaaccagg
    47821 aagggtcgca gacgcttacc acctaataat gcaccatact gcatggtttc gaccacggga
    47881 gtgttctgaa agggcagtgg ggcgataaaa cggctcagcg cctggttggc ctgcttaacg
    47941 caggcttcga gttgctgcgg aaagtccatt actcattgtc cggtgtaaaa ggggttagag
    48001 aggcgtcttc attgtcagac agcagaattt gtacgcgctg ttcggcttgt tgtaatttgg
    48061 cctgcccctg acgtgccagc tgcacgccgc gttcgaactc gttcagcgcc tcttccagcg
    48121 gcaggtcgcc actttccaga cgggttacaa tctgttccag ctcgctcagc gccttttcaa
    48181 agctggcggg agcctcattt ttcttcggca taatgaatgt ctgactctca atatttttcg
    48241 ccccgtcatg gtaacggact cagggcaaat agcaaataac gcgcaatggt aaggtgatgt
    48301 gcgcagcaaa gcgatgttag tggtatactt ccgcgcctgg atgcagccgc aggtgtgggc
    48361 tgctgtattt ttccctatac aagtcgctta aggcttgcca acgaaccatt gccgccatga
    48421 agtttatcat taaattgttc ccggaaatca ccatcaaaag ccaatctgtg cgcttgcgct
    48481 ttataaaaat ccttaccggg aacattcgta acgttttaaa gcactatgat gagacgctcg
    48541 ctgtcgtccg ccactgggat aacatcgaag ttcgtgcaaa agatgaaaac cagcgtctgg
    48601 ctattcgcga cgccctgacc cgtattccgg gtatccacca tattctcgaa gtcgaagacg
    48661 tgccgtttac cgacatgcac gatatttttg agaaagcgtt ggttcagtat cgcgatcagc
    48721 tggaaggcaa aaccttctgc gtacgcgtga agcgccgtgg caaacatgat tttagctcga
    48781 ttgatgtgga acgttacgtc ggcggcggtt taaatcagca tattgagtcc gcgcgcgtga
    48841 agctgaccaa tccggaagtg actgtccatc tggaagtgga agacgatcgt ctcctgctga
    48901 ttaaaggccg ctacgaaggt attggcggtt tcccgatcgg cacccaggaa gatgtgctgt
    48961 cgctcatttc cggtggtttc gactccggtg tttccagtta tatgttgatg cgtcgcggct
    49021 gccgcgtgca ttactgtttc tttaaccttg gcggcgcggc gcatgaaatt ggcgtgcgtc
    49081 aggtggcgca ttatctgtgg aaccgttttg gcagctccca ccgcgtgcgt tttgtcgcta
    49141 ttaatttcga accggtcgtc ggggaaattc tcgagaaaat cgacgacggt cagatgggcg
    49201 ttatcctcaa acgtatgatg gtgcgtgccg cgtctaaagt ggctgaacgt tacggcgtac
    49261 aggcgctggt caccggcgaa gcgctcggcc aggtgtccag tcagacgctg accaacctgc
    49321 gcctgattga taacgtctcc gatacgctga tcctgcgtcc gctgatttct tacgacaaag
    49381 agcacatcat caacctggct cgccagattg gcaccgaaga ctttgctcgc acgatgccgg
    49441 aatattgcgg tgttatctcc aaaagcccga cggtgaaagc ggttaagtcg aagattgaag
    49501 cggaagaaga gaaattcgac ttcagcattc tcgataaagt ggttgaagaa gcgaataacg
    49561 ttgatatccg cgaaatcgcc ctgcagaccg agcaggaagt ggtggaagtg gaaaccgtca
    49621 atggcttcgg cccgaacgac gtgatcctcg atatccgttc tatcgatgaa caggaagata
    49681 agccactgaa agtcgaaggg attgatgtag tttctctgcc gttctataaa ctgagcacca
    49741 agtttggcga tctcgaccag aacagaacct ggctactgtg gtgtgagcgc ggggtgatga
    49801 gccgtctgca ggcgctctat ctgcgcgagc agggctttaa caatgtgaag gtatatcgcc
    49861 cgtaatttgt ggttgtaacg tcgcatctgg tcagatgcga cgcttaccgc atccgacact
    49921 actcgtaata attataaatc cctgccgcca tcaccagttg tgatgccact tcatgggctt
    49981 tctcgcgccc aacctgcagg tcgataattt tcagaccaaa gtcgatagct gtccccggcc
    50041 cctggctggt cagcaatttt acccgtgcat cccagacgac gcgcttgtcc tgccattgtt
    50101 cagcgggaat tttgtctttc agcgtcggga agccggtcat attaccaatc gggaagatat
    50161 cgtgcggcac cagcacggtg gctggcgcgg cgcaaatagc cgcgacgata cgcccggaac
    50221 ggtggaactg tttaacggtt tcaaccagca gagtgctatc gcgaaaacac tctgcgcctt
    50281 taatgccacc aggcagtacg atcacgtcat attcgccatc agccacctcg accagcggcg
    50341 catccgccag cagcttcacg ccgcgcgagc aggtaattgc caggttgcca tcgctggcta
    50401 cgctggcagt ggtgactttg ataccgccgc gaaccaagag gtcgatcgtg gtgacggctt
    50461 cagtctcttc actaccaggg gcgaggcaaa ccagtgccga tgcgctcata ttcactctcc
    50521 tttcttttta ccatttcaaa caggcgggtg ttttccggta cggcaatccc atgcgcgcgg
    50581 gcgcggcgta agagaaaacc attgatatag tcgatttcgg tgtggcgcag cgcgcggata
    50641 tcctgcaaca tcgacgagat attttccgct gtggcatcaa tcacctgcat cacgtaatca
    50701 cgcaaatctt ccgctgaagt atgatgccct tcgcgttcga tcactgccgc gacttcttcg
    50761 catatttgca taatttcttg cggatgatga cgtaattcac cgttcgggca attccagatg
    50821 gcggtcagtg gattaatcac gcagttgact gccagcttgc gccacagctc ggcgcgaata
    50881 ttgttatgcc aggcaacgtc aggcaacacg gtttgtaaaa tatccgccag ataactgtaa
    50941 tcgccatcct gttgccgtgc cggaccaata tgcgtgatac cgtttgccac atgaataatg
    51001 acattgccgt cgcggcgggc ggcatgggtg gtggtgccca tcagtaatgg ctgctgaata
    51061 ttttgcaact cttcgatggt gcccatgccg ttgtgaatta acagtattgg cgtggttaca
    51121 ggcagtgtgg acgcgaggct tttgacggca tcggaaacct gccatgcttt cagcgtcacc
    51181 aggagcagat cgctggtggc gagaaaatcg ggatcgttgg cggtcagcga ttcgttaaat
    51241 atcgaaccat ctgtctcaac cagattcgca ctacaataag gttgcggtac gcgcagccag
    51301 ccctgaactt catgaccctg tttgcaaagt gctgtaagcc ataattgccc taaggcaccg
    51361 catcccaata cggtaatttt cattgttcct cctcacccgc aaccactccg ggtgttcaat
    51421 aaggctatcc cttaattgtg catgctgttg cgactatgca caattaaggg atacgtcctg
    51481 gtgcaggact gtcggttatt taactttgcg ggtattatgc ttcgcatcaa aaatgaaggg
    51541 agaggaaaag atgccatctt tcgatattgt ctctgaagtt gatcttcagg aagcacgtaa
    51601 cgcggtcgat aacgcgagcc gcgaagtgga gtcccgtttt gacttccgta acgttgaagc
    51661 ctcatttgag ctgaatgacg ccagcaaaac catcaaagtg ttgagcgagt ccgatttcca
    51721 ggtcaatcag ttgctggata ttctgcgtgc caagctgctg aagcgcggca ttgaaggcag
    51781 ttcactggat gtaccggaaa atatcgttca tagcggtaaa acctggtttg tggaagcgaa
    51841 actgaagcag ggcattgaga gcgcgaccca gaaaaaaatc gtcaagatga tcaaagacag
    51901 caaactgaaa gtgcaggcgc aaattcaggg cgatgagatc cgcgtgactg gcaaatctcg
    51961 tgatgatttg caggctgtca tggcgatggt gcgcggtggc gatctgggtc agccgttcca
    52021 gttcaaaaac ttccgcgatt aatcgcgaca acctgcgctt tgttcatgtc agatgcggca
    52081 tgaacgcctg gttcggccta caaaggcttg taaatcagga gcttagtagg cctgatgagc
    52141 gtagcgcagc aggcaatttc gcctcgcttt tatgcctggc gaattgcctg ttcaacatca
    52201 aagcgattcg tcactttgct gtcgattttc acataagctg aatgttcttc ttctgcaatc
    52261 aacacttctt tgatgccttc agtttctagc aaacgcactt ttaacgcctc gtttgcggca
    52321 atgttcgccg ggatttcaat gcgcaaactg ctgacatacg ccggttcttt catggtactg
    52381 gcgactgcca gccacactgc ggccagcatt gcgccagcga gaaatacgcc ctgaccgtca
    52441 aacatgccgt caatccagcc gcccagtgaa ccgccaatcg ccacgccaag aaactggctg
    52501 gtggagtaaa cacccatcgc tgtacctttg taacctgctg gcgactcttt actgataagt
    52561 gaaggcagaa gggcttccat caaattaaac gctacaaaga aaagctgcac gccgaccacc
    52621 agttgccaga actgcgtttg cgcgttccac aacacaattt ccgcaaccac gatcaatccg
    52681 acgcagaaga caaagacttg cttcattttg cgcttaactt cagcgtagat aatgaaaggc
    52741 acgaccgagc caaaggcgat tagcattgtc gccagataga ccttccagtg ttcagccgcc
    52801 gggaaccctg catcagccag ttgtccgggc agggcaacaa acgtcgacat cagcaaaata
    52861 tgcagacaca taatgccaaa gttgagtttc agcagccgcg gttccgccag cactttactg
    52921 aaactgcctt tcaccattcc ggactcacga ttaagtacgt gagtgctact gttgggcaca
    52981 acccaaatgg tcaacgcaat gccggtcgtt gccagaatag cgatcatcca gaacagcgcg
    53041 tgcagcccaa gtttgtgagt gatgatcggg ccaagcacca tcgcaatggc aaaggtaatg
    53101 ccaaagctca cgccgataaa cgccattgct ttggtgcggt tttgttcgcg cgtgagatcg
    53161 gaaagcagcg ccataacggc agcggcaatc gcaccggagc cttgtagcgc ccggcctaga
    53221 ataattcccc agatggagtc agagagcgca gcgataacgc taccggcggc aaacaccgcc
    53281 agcccaccga caattaatgg tttgcgacca atacggtcgg aaagcaggcc aaacggaatc
    53341 tgaaaaacgg cctgagtcag accataaata ccaatggcaa taccgattaa tgcttcgctg
    53401 gcaccttgca gagccatgcc atacgtggtc agaaccggca gaaccataaa catgcccagc
    53461 atgcgcaacg agaatacggt ccctaaaccc caggtcgcgc gcctctcacc tggcgtcatt
    53521 ttataatcgt tcattaccac ctctgtttta aattcgcgac tagtgtaaag cggcaaacga
    53581 atggggtaaa catgcggatt ttttagtaaa tatttcgaga gggatatgtt tctaatgcta
    53641 agaaaaaagg tgccgtagca cctttttaat agagaggttt tgttaccaca cagcagccag
    53701 cagcgtatgc gagtccggta ccataaaatc aacggacatc atcaccgaga gggcagtgat
    53761 ggcgatgata gagaagccga acagcttgcg cgcccagatt ctgtcatcag caactttata
    53821 accgcgcaga gccataccta accaccagac gctaaccgcc gcggcgacca ccagatattt
    53881 atatccagcg taaccgccaa gagagagcat cagcgtggca acggcaaagg cgatgatata
    53941 cagcgtgatg tgattcttcg ccaccgaaat gccttttacc actggcaata ccggaatgtt
    54001 tgccgcctgg taatccttaa agcggaaaat ggcgatggca taggagtgag gcatctgcca
    54061 caggctgaag atagccagca ggatcgctgc gccgctatcg aactcgccgg ttactgcaca
    54121 gtagccaatc accggcggcg cagcgccgga gagcgaacca atcaatgtgc cgtagactga
    54181 gtggcgtttc atgtacaggc tatagacgcc gacataaacc acaaagccca tcacccccag
    54241 ccagcaggcc agcggattcg cgccaaacca cagcagcata aagccagcaa tacccagcaa
    54301 ggtggcgtac accagcgaga cagcaggaga gatcaggcct ttaaccagca cccgattctt
    54361 cgtcctttcc atctttctgt cgatatccct gtcgatgtag ttgttaaaca cacaacccga
    54421 cgccacaacc agtgacaccc caaccagcgt gtagataaac aggggataat caatgctgcc
    54481 ctttgaggcc agcaggaatc ccccaatcac cgagatcagg ttgccaaaga tgatgcctgg
    54541 tttcgttact tgcaggtatt gcttaaacat cataaccgcc gctcttagtg catcatcatg
    54601 ttgtagttga ggttccacat aatccagatg gagcctacaa ccaggatagc gatgattagc
    54661 acggtgaaga caaacgccgt catgttccag ccttcatctg atttggtatt catgtgcagg
    54721 aagcacacca gatgcaccag aacctgtacc actgccattg ccaggattgt tcccagaatt
    54781 acggccggag aggctgcccc tgtcatcacc atccagaacg gaatcaccgt cagaatgatc
    54841 gacaggataa agcctgtcat gtaggttttt acgctgccat gggacgcgcc gctgtgatcg
    54901 gtagaatgac tcattacatc gcccccatca gataaacaac agtgaacaca cagatccaaa
    54961 ccacatccag gaagtgccag aacaggctca ggcacatgat gcgggtacgg ttagtgctgg
    55021 tcaggccgcg acgagcgatt tgtaccatca gcaccgccat ccagataaga ccggaagtga
    55081 cgtgcagacc gtgcgtgccg accagcgcaa agaacgctga caggaagccg ctgcgatccg
    55141 gacccatgcc gttaacaatc aggtgatgga attcgtagat ttccatcccg ataaatccgg
    55201 caccaaacaa acaggtcaac gccagccagg agataacctg gcttttgttg tttttgtaca
    55261 tggcgatagc cgccatgccg taggtgatgg agctgaacaa cagcaagaaa gtttcaacca
    55321 gaacgaacgg cagttcgaaa atgtccttac ctgtcgggcc gcctgcggtg ccgtttacca
    55381 gaacggcata ggtagcaaac aagattgaga acagaatgca gtcgctcatc aggtagatcc
    55441 aaaatccgaa gattttggtt ccgcctgcat cgtggtgccc gtgttcgtgc gcgtgggcag
    55501 tcgcgtgcgt caaagtatca gttgccattt ttcagccctg ccttagtaat ctcatcgaaa
    55561 tgctggtttt ccagtttttc gatttctgcc accggaacgt agtaatccac gtcctcgtcg
    55621 aagcttttca cgatccaggt gatgatcatg cctgcgaagc caacaatcgc cagccaccag
    55681 atatgccaga tcatggcgaa accgaagatg gtggagaaag ctgcaatgac gatacctgca
    55741 ccgctgtttt tcggcatatg aatttcttca tagtggtcag gctttttata cgcttcgcct
    55801 ttctctttca tttcccagaa tgcatcacgc tcgtgaacgt gcggcacaac ggcaaagtta
    55861 tagaacggag gcggggaaga gtttgcccac tccagcgtac ggccacccca cgggtcgcca
    55921 gtcaggtcac ggttctggtc gcggtcgcga atagaaacgt acatctgaat aacgaggcag
    55981 agaataccca gcgcaatcag tactgcaccg ctagctgcaa tcatcagcat ggtgtggaac
    56041 tgcgggtcaa tctgctggct caaacgacgg gtcatgccca tgaagcccag cgcatacagc
    56101 ggcataaagg caacgaagaa gccgatgatc cagaaccaga acgcgcgttt accccaggtt
    56161 tcattcagtt tgaaaccgaa cgctttaggc caccagtagg tcatccctgc gaagcagccg
    56221 aagaccacgc cgccgatgat aacgttatgg aagtgcgcaa tcaggaacag gctgttatgc
    56281 agaacgaagt ccgcgcccgg tactgccagc agcacgccag tcatcccgcc caccgagaaa
    56341 gtgacgataa aaccgatggt ccacagcatc gcagaatgga acacgatacg gccctgatac
    56401 atggtgaaca gccagttgaa gatcttcacc ccggtcggga tggcgataat cattgtggtg
    56461 ataccaaaga aggcgtttac gttcgcgccc gcacccatcg taaagaagtg gtgcagccaa
    56521 acgatgaacg acagcacggt gatacagacg gttgcccata ccagcgaggt ataaccaaac
    56581 agacgtttac gcgagaaggt tgccgcaatt tcggagaaca caccgaaaac aggcaggatc
    56641 aggatgtaaa cttccgggtg gccccaggcc caaatcaggt tgatgtacat catcatgttg
    56701 ccacccatat cgttggtaaa gaaatgggtg cccagatagc gatccagggt caacaacgcg
    56761 acggtaaccg tcagaattgg gaaggaagca ataatcagta cgttcgcgca cagtgatgcc
    56821 caggtaaata ctggcatctt gaacatggtc atgcccggtg cgcgcatctt cagaatggta
    56881 acgaagaagt tgataccggt aagcgtcgta cctataccgg atagctggag actccatatc
    56941 cagtaatcaa caccgactcc cggactgtac tctattcccg atagcggtgg ataggccagc
    57001 cagccggtct gcgcaaattc gcccacgccg agagaaacgt taaccagaat cacaccaaca
    57061 acagtaaacc agaagcttaa gttgttgagg aacgggaacg caacgtcacg cgcgccgatc
    57121 tgcagcggaa ccaccaggtt catcagaccg ataacgaaag gcatcgccac gaagaagatc
    57181 ataatcacgc cgtgcgcggt gaagatctga tcgtagtggt gaggtggcag gaagcccgct
    57241 tcgcccgccg aggcaagagc ctgctggcta cgcatcataa tggcgtcagc aaaaccacgc
    57301 agcaacatca caatcgccac gatgatatac atgataccga ggcgtttatg gtcgacggag
    57361 gtcagccact ctttccacag gtaggtccac ttaccgaagt aagtgatcag gccaacgagc
    57421 gccagacctc ccaaaataat gccagcgatc gtaaccatga cgataggttc atggaacggg
    57481 actgcatcaa gtgataattt tccgaacatc tttattcttc ctcaacccct ttaatgggcg
    57541 gattccgcgt ggctcatgtc catgccttcc ataccttcgt gtgcgctgtg ctcaccttct
    57601 ggctgggtca tgtccatgct cttaccgtga gccataaact tgttaatcac atcagcaaac
    57661 aagtctggtt tcacgttgga gaaatattcc acctggttgt attcgctagg cgcggccagt
    57721 ttttcgaacg cagccatgtc agacatggtg ttcggcgact gcttcgcttt tgcgacccac
    57781 tggtcgaatt cggcgcgatc cggtgttgca atagctttga acttcatgcc tgagaagccc
    57841 gggccgctgt agctggcgga gataccgtca taagtaccgg gttcgttggc gatcagatgc
    57901 aggcgagtct gcataccagc catggcataa atctggctac ccagacgcgg aatgaagaag
    57961 gagttcatca cggagttgga ggtcactttg aagtacaccg gagtgttcgc cgggaaagcg
    58021 atttcattca cggtagcaat gccctgttcc gggtagatga agaaccattt ccagtccatg
    58081 gaaaccactt cgatggtaat gggcttctcg tcgtgtgcca gcggcttgct aggctcaaga
    58141 gcgtgagtgg ttttccaggt cagtaccgca aggaagatga tgattaagat aggtaccgtc
    58201 cagaccacag cttccacttt attggagtgt gaccagttcg ggctgtactt agcatcttta
    58261 ttgctcgcac gatacttcca ggcgaaacca acagccatca agattgcggg aataacgaca
    58321 atcaacatca ggccaaatgc cgtcagtatc agtgaacgtt gctccagacc aatctgtcct
    58381 ttgggatcta acagcgcaga attacagcca ctgagcaata cagtgcctgc aaataatgac
    58441 aaccatccca aacttttatt gtatttcctg agtctcattt aacgacctca attccacggg
    58501 atctggtggc gtttaaagtg tgtgggcatt ttatgggaag gttacattac tgtaaacatg
    58561 attaaattcc tgttacttgg atttgactgt caggtcacat atgttgcaaa acatatcaag
    58621 ttgattttaa gaagctgttg caaaagggcg ttataacgaa aggggagtgc agaaggagtt
    58681 tggacgggca attggtataa ccaatctgga ataaaataat tgtttaacaa ataattatca
    58741 ttatggtgac aattgggtgg ggggaaaata aagactcgtt agctgaatcg tgtaagaaaa
    58801 aatagaattc ataaattgcg tcaataattt ccaaacgcta agccgcacaa aagaacaaat
    58861 attattaagc gatactgatg tttatttagc cgtaataatt acggcgagtg attactacag
    58921 ctaaataata tttacagatt acgtcagatg cgtttttcgt agagccagat aatccagcaa
    58981 accaccaagc acgacgccag aaagggcgac taaaaccccg acttccagca gagcaggcag
    59041 gaaagagaag tgcgtcaaat ccagcgcgtc catcgtcaac agcagtaacc acacggccaa
    59101 caggctgacg cccgccgcca gtgtccacat ggcaaaggca taacctgccg gatattcggt
    59161 acgggagata aagttgtcat ttactcgtgt atattcaagc gtctggcggc aaaccagcag
    59221 caaaataagc cccggtacgg cagcggcgac ggagaataga tagaatgtcg accagccgtg
    59281 tgcttcaaca aaccaacccg ccacggggcc gacataaact cgtcctacag cggaaagcgc
    59341 tgagagcagg gcaaattgag tagcggaaaa tgacttatta cacagcgtca ttaacagcgc
    59401 gacaaaggct gatgtgccca tcccgccaca gaggttttcg aaaaagacgg ctgcgcccat
    59461 gctgtagaga tgcttatcag taatcgacag cagccagtaa ccagcgttag acgcaccttg
    59521 taaaatgccg aaaatcagca gcgcccggaa cagcgacagg cgctgcatca aaatcccacc
    59581 gtacaatgcg cccacaatgg tcgccagtaa gccaagcgtt ttgttaacca cgcctacttc
    59641 acccgcatca aacccgacgc cgcgaatcaa aaacgtggtt gtcaggctca tggcgaatgc
    59701 gtcgcccagc ttatacagca cgataagaag caaaataagc caggcattat tgcgaccaaa
    59761 gaaatctcgc agaggtgcaa caaccgcttg ttccagcgtt tttggcacag gaatggtgtc
    59821 ggtcggttct ggtgcaagca acgtcgcgat aatacaaggg atcaacagcg ccgccatcaa
    59881 ccagtacatg ccctgccagc ccagccattt atctgccagc cacagggtta agccgccgga
    59941 aaccagcatc cctaaacggt aacccagcac gctgattgcc gcgcccgcac cacgttcttc
    60001 tgccgggagc acatcggttt tccacgcatc gaagacaata tcctgggagg ccgagcaaaa
    60061 agcgatcacc accgccagtg ccgccatcca gcggagttgg gtgccaggtt cgagaaaacc
    60121 catcgccgca atggcgacta ataacaggat ttgcgtggcg agcagccaac cgcgccgccg
    60181 cccaaaaaat gggggcgtgt agcggtccat cagcggtgac cagaggaatt taaaaacgta
    60241 agcctggcct accagagaga agaaaccaat ggttttgaga tcaatattct cgaccgtcat
    60301 ccaggcctgt aaggtaccgg atgtcagggc gagcggtagc ccggaagcaa aacccaggat
    60361 cagcaatatg gctgaacgcg gctgttgaaa aatacgtaaa tattgactgg acatgggcgt
    60421 ctacaggccc ggtttgcacc gggccagagg gcagaaatta acgcgcgttc tgcttgatga
    60481 attcgtgaat gctggtgtcc tgagacatat cagcgatggt atcggtcagc acactgttaa
    60541 ccgcatcggc gatatttttg ttggaggcct ggaacgcacc ttcaacgttg tagctggcac
    60601 gatagttttt ggttattttg ttgccattct gcgcggtagc gatgatggcg atatccgctt
    60661 tggtagcgat gttgtagcgc acgttgccct gggacacgtc agcatacagt tggctaacga
    60721 tgatttgcag attaaccggg ccattcggac caaccatgta accacgcgcg gtcatctgtt
    60781 tttccagcac ttcttgcagc aggaaacgca gatcgcggga ggcggccagg gtaacgattt
    60841 gattatcgcg ggtgactttt gccagcgcct gatcggtacg ctgatcggca ccattaatgc
    60901 ttacggtgac gcccatcagg cttggatcct gctgtggcag tgtaatcgtc ggggaaactt
    60961 caatagttgt tggcggtttt gcgcatcctg caagcataaa cagagcaact aacgggaaga
    61021 ggattttttt gaacatgttc gggctctcag agactcttaa gcgtgtttgg taaaaattcc
    61081 cgccatcata acattgccaa cggcgagggg aagtgggtaa ggcatgtaaa ttcatcatgt
    61141 tgacgaaata atcacccctg gtaaaagaaa cgctgatgcg aggcctgtgt ttcaatcttt
    61201 aaatcagtaa acttcatacg cttgacggaa aaactaggac gaaacctaaa tatttgttgt
    61261 taagctgcaa tggaaacggt aaaagcggct agtatttaaa gggatggatg acatctcagc
    61321 gttgtcggag gagatatttc atgatgatac gtgagcggat agaagaaaaa ttaagggcgg
    61381 cgttccaacc cgtattcctc gaagtagtgg atgaaagcta tcgtcacaat gtcccagccg
    61441 gctctgaaag ccattttaaa gttgtgctgg tcagcgatcg ttttacgggt gaacgttttc
    61501 tgaatcgtca tcgaatgatt tacagtactt tagcggagga actctctact accgttcatg
    61561 cgctggctct gcatacttac actattaagg agtgggaagg gttgcaggac accgtctttg
    61621 cctctcctcc ctgtcgtgga gcaggaagca tcgcgtaaaa acgcatttgc aactgtcggc
    61681 gctttttcag tatgttgcta aagattttat gaaaaacggc ctgcgggccg ttttgttttg
    61741 tctggatttt gcgctttttg cccagcattc agacgaaaat tgcccgggaa ttgtgaaaaa
    61801 atacgcgaca gcgcgcaata accgttctcg actcataaaa gtgatgccgc tataatgccg
    61861 cgtcctattt gaatgctttc gggatgattc tggtaacagg gaatgtgatt gattataaga
    61921 acatcccggt tccgcgaagc caacaacctg tgcttgcggg gtaagagttg accgagcact
    61981 gtgatttttt gaggtaacaa gatgcaagtt tcagttgaaa ccactcaagg ccttggccgc
    62041 cgtgtaacga ttactatcgc tgctgacagc atcgagaccg ctgttaaaag cgagctggtc
    62101 aacgttgcga aaaaagtacg tattgacggc ttccgcaaag gcaaagtgcc aatgaatatc
    62161 gttgctcagc gttatggcgc gtctgtacgc caggacgttc tgggtgacct gatgagccgt
    62221 aacttcattg acgccatcat taaagaaaaa atcaatccgg ctggcgcacc gacttatgtt
    62281 ccgggcgaat acaagctggg tgaagacttc acttactctg tagagtttga agtttatccg
    62341 gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa aaccgatcgt tgaagtgacc
    62401 gacgctgacg ttgacggcat gctggatact ctgcgtaaac agcaggcgac ctggaaagaa
    62461 aaagacagcg ctgttgaagc agaagaccgc gtaaccatcg acttcaccgg ttctgtagac
    62521 agcgaagagt tcgaaggcgg taaagcgtct gatttcgtac tggcgatggg ccagggtcgt
    62581 atgatcccgg gctttgaaga cggtatcaaa ggccacaaag ctggcgaaga gttcaccatc
    62641 gacgtgacct tcccggaaga ataccacgca gaaaacctga aaggtaaagc agcgaaattc
    62701 gctatcaacc tgaagaaagt tgaagagcgt gaactgccgg aactgactgc agaattcatc
    62761 aaacgtttcg gcgttgaaga tggttccgta gaaggtctgc gcgctgaagt gcgtaaaaac
    62821 atggagcgcg agctgaagag cgccatccgt aaccgcgtta agtctcaggc gatcgaaggt
    62881 ctggtaaaag ctaacgacat cgacgtaccg gctgcgctga tcgacagcga aatcgacgtt
    62941 ctgcgtcgcc aggctgcaca gcgtttcggt ggcaacgaaa aacaagctct ggaactgccg
    63001 cgcgaactgt tcgaagaaca ggctaaacgc cgcgtagttg ttggcctgct gctgggcgaa
    63061 gttatccgca ccaacgagct gaaagctgac gaagagcgcg tgaaaggcct gatcgaagag
    63121 atggcttctg cgtacgaaga tccgaaagaa gttatcgagt tctacagcaa aaacaaagaa
    63181 ctgatggaca acatgcgcaa tgttgctctg gaagaacagg ctgttgaagc tgtactggcg
    63241 aaagcgaaag tgactgaaaa agaaaccact ttcaacgagc tgatgaacca gcaggcgtaa
    63301 tttacgcagc ataacgcgct aaattcgcac aaaggcccgt caccgccagg tggtgggctt
    63361 tttttgtcat gaattttgca tggaaccgtg cgaaaagcct ctttcggtgt tagcgtaaca
    63421 acaaaagatt gttatgcttg aaatatggtg atgccgtacc cataacacag ggactagctg
    63481 ataatccgtc cataaggtta caatcggtac agcaggtttt ttcaatttta tccaggagac
    63541 ggaaatgtca tacagcggcg aacgagataa ctttgcaccc catatggcgc tggtgccgat
    63601 ggtcattgaa cagacctcac gcggtgagcg ctcttttgat atctattctc gtctacttaa
    63661 ggaacgcgtc atttttctga ctggccaggt tgaagaccac atggctaacc tgattgtggc
    63721 gcagatgctg ttcctggaag cggaaaaccc agaaaaagat atctatctgt acattaactc
    63781 tccaggcggg gtgatcactg ccgggatgtc tatctatgac accatgcagt ttatcaagcc
    63841 tgatgtcagc accatctgta tgggccaggc ggcctcgatg ggcgctttct tgctgaccgc
    63901 aggggcaaaa ggtaaacgtt tttgcctgcc gaattcgcgc gtgatgattc accaaccgtt
    63961 gggcggctac cagggccagg cgaccgatat cgaaattcat gcccgtgaaa ttctgaaagt
    64021 taaagggcgc atgaatgaac ttatggcgct tcatacgggt caatcattag aacagattga
    64081 acgtgatacc gagcgcgatc gcttcctttc cgcccctgaa gcggtggaat acggtctggt
    64141 cgattcgatt ctgacccatc gtaattgatg ccagaggcgc aactgtgccg ctatacttat
    64201 ccagggcggc acaacgctgt aagcggcttg cgcctgagaa tggcatttgc gtcgtcgtgt
    64261 gcggcacaaa gaacaaagaa gaggttttga cccatgacag ataaacgcaa agatggctca
    64321 ggcaaattgc tgtattgctc tttttgcggc aaaagccagc atgaagtgcg caagctgatt
    64381 gccggtccat ccgtgtatat ctgcgacgaa tgtgttgatt tatgtaacga catcattcgc
    64441 gaagagatta aagaagttgc accgcatcgt gaacgcagtg cgctaccgac gccgcatgaa
    64501 attcgcaacc acctggacga ttacgttatc ggccaggaac aggcgaaaaa agtgctggcg
    64561 gtcgcggtat acaaccatta caaacgtctg cgcaacggcg ataccagcaa tggtgtcgag
    64621 ttgggcaaaa gtaacattct gctgatcggt ccgaccggtt ccggtaaaac gctgctggct
    64681 gaaacgctgg cgcgcctgct ggatgttccg ttcaccatgg ccgacgcgac tacactgacc
    64741 gaagccggtt atgtgggtga agacgttgaa aacatcattc agaagctgtt gcagaaatgc
    64801 gactacgatg tccagaaagc acagcgtggt attgtctaca tcgatgaaat cgacaagatt
    64861 tctcgtaagt cggacaaccc gtctattacc cgtgacgttt ccggtgaagg cgtacagcag
    64921 gcactgttga aactgatcga aggtactgtt gctgccgttc caccgcaggg cgggcgtaag
    64981 catccgcagc aggaattttt gcaggttgat acctccaaga tcctgtttat ttgtggcggt
    65041 gcgtttgccg gtctggataa agtgatttcc caccgtgtag aaaccggctc cggcattggt
    65101 tttggcgcga cggtaaaagc gaagtccgac aaagcaagcg aaggcgagct gctggcgcag
    65161 gttgaaccgg aagatctgat caagtttggt cttatccctg agtttattgg tcgtctgccg
    65221 gttgtcgcaa cgttgaatga actgagcgaa gaagctctga ttcagatcct caaagagccg
    65281 aaaaacgccc tgaccaagca gtatcaggcg ctgtttaatc tggaaggcgt ggatctggaa
    65341 ttccgtgacg aggcgctgga tgctatcgct aagaaagcga tggcgcgtaa aaccggtgcc
    65401 cgtggcctgc gttccatcgt agaagccgca ctgctcgata ccatgtacga tctgccgtcc
    65461 atggaagatg tcgaaaaagt ggttatcgac gagtcggtaa ttgatggtca aagcaaaccg
    65521 ttgctgattt atggtaagcc ggaagcgcaa caggcatctg gtgaataatt aaccattccc
    65581 atacaattag ttaaccaaaa aggggggatt ttatctcccc tttaattttt cctctattct
    65641 cggcgttgaa tgtgggggaa acatccccat atactgacgt acatgttaat agatggcgtg
    65701 aagcacagtc gtgtcatctg attacctggc ggaaattaaa ctaagagaga gctctatgaa
    65761 tcctgagcgt tctgaacgca ttgaaatccc cgtattgccg ctgcgcgatg tggtggttta
    65821 tccgcacatg gtcattccct tatttgtcgg gcgggaaaaa tctatccgtt gtctggaagc
    65881 ggcgatggac catgataaaa aaattatgct ggtcgcgcag aaagaagctt caacggatga
    65941 gccgggtgta aacgatcttt tcaccgtcgg gaccgtggcc tctatattgc agatgctgaa
    66001 actgcctgac ggcaccgtca aagtgctggt cgaggggtta cagcgcgcgc gtatttctgc
    66061 gctctctgac aatggcgaac acttttctgc gaaggcggag tatctggagt cgccgaccat
    66121 tgatgagcgg gaacaggaag tgctggtgcg tactgcaatc agccagttcg aaggctacat
    66181 caagctgaac aaaaaaatcc caccagaagt gctgacgtcg ctgaatagca tcgacgatcc
    66241 ggcgcgtctg gcggatacca ttgctgcaca tatgccactg aaactggctg acaaacagtc
    66301 cgttctggag atgtccgacg ttaacgaacg tctggaatat ctgatggcaa tgatggaatc
    66361 ggaaatcgat ctgctgcagg ttgagaaacg cattcgcaac cgcgttaaaa agcagatgga
    66421 gaaatcccag cgtgagtact atctgaacga gcaaatgaaa gctattcaga aagaactcgg
    66481 tgaaatggac gacgcgccgg acgaaaacga agccctgaag cgcaaaatcg acgcggcgaa
    66541 gatgccgaaa gaggcaaaag agaaagcgga agcagagttg cagaagctga aaatgatgtc
    66601 tccgatgtcg gcagaagcga ccgtagtgcg tggttatatc gactggatgg tacaggtacc
    66661 gtggaatgcg cgcagcaagg tcaaaaaaga cctgcgtcag gcgcaggaaa tccttgatac
    66721 cgaccattat ggtctggagc gcgtgaaaga tcgcatcctt gagtaccttg cggttcaaag
    66781 ccgtgtcaac aaaatcaagg gaccgatcct ctgcctggta gggccgccgg gggtaggtaa
    66841 aacctctctt ggtcagtcca ttgccaaagc caccgggcgt aaatatgtcc gtatggcgct
    66901 gggcggcgtg cgtgatgaag cggaaatccg tggtcaccgc cgtacttaca tcggttctat
    66961 gccgggtaaa ctgatccaga aaatggcgaa agtgggcgtg aaaaacccgc tgttcctgct
    67021 cgatgagatc gacaaaatgt cttctgacat gcgaggcgat ccggcctctg cactgcttga
    67081 agtgctggat ccagagcaga acgtagcgtt cagcgaccac tacctggaag tggattacga
    67141 tctcagcgac gtgatgtttg tcgcgacgtc gaactccatg aacattccgg caccgctgct
    67201 ggatcgtatg gaagtgattc gcctctccgg ttataccgaa gatgaaaaac tgaacatcgc
    67261 caaacgtcac ctgctgccga agcagattga acgtaatgca ctgaaaaaag gtgagctgac
    67321 cgtcgacgat agcgccatta tcggcattat tcgttactac acccgtgagg cgggcgtgcg
    67381 tggtctggag cgtgaaatct ccaaactgtg ccgcaaagcg gttaagcagt tactgctcga
    67441 taagtcatta aaacatatcg aaattaacgg cgataacctg catgactacc tcggtgttca
    67501 gcgtttcgac tatggtcgcg ctgataacga aaactgtgtc ggtcaggtaa ccggtctggc
    67561 gtggacggaa gtgggcggtg acttgctgac cattgaaacc gcatgtgttc cgggtaaagg
    67621 caaactgacc tataccggtt cgctcggcga agtgatgcag gagtctattc aggcggcgtt
    67681 aacggtggtt cgtgcgcgtg cggaaaaact ggggatcaac cctgattttt acgaaaaacg
    67741 tgacatccac gtccacgtac cggaaggtgc gacgccgaaa gatggtccga gtgccggtat
    67801 tgctatgtgc accgcgctgg tttcttgcct gaccggtaac ccggttcgtg ccgatgtggc
    67861 aatgaccggt gagatcactc tgcgtggtca ggtactgccg atcggtggtt tgaaagaaaa
    67921 actcctggca gcgcatcgcg gcgggattaa aacagtgcta attccgttcg aaaataaacg
    67981 cgatctggaa gagattcctg acaacgtaat tgccgatctg gacattcatc ctgtgaagcg
    68041 cattgaggaa gttctgactc tggcgctgca aaatgaaccg tctggcatgc aggttgtgac
    68101 tgcaaaatag tgacctcgcg caaaatgcac taataaaaac agggctggca ggctaattcg
    68161 ggcttgccag cctttttttg tctcgctaag ttagatggcg gatcgggctt gcccttatta
    68221 aggggtgttg taaggggatg gctggcctga tataactgct gcgcgttcgt accttgaagg
    68281 attcaagtgc gatataaatt ataaagagga agagaagagt gaataaatct caattgatcg
    68341 acaagattgc tgcaggggct gatatctcta aagctgcggc tggccgtgcg ttagatgcta
    68401 ttattgcttc cgtaactgaa tctctgaaag aaggggatga tgtagcactg gtaggttttg
    68461 gtacttttgc cgttaaagag cgtgctgccc gtactggccg caacccgcag accggtaaag
    68521 agatcaccat cgctgctgct aaagtaccga gcttccgtgc aggtaaagca ctgaaagacg
    68581 cggtaaacta agcgttgtcc ccggtgggga tgtgacgaag ttcaagggcg catctactga
    68641 tgtgcctttt ttatttgtat tcggtgactt tctgcgtctt gtgggctgac aattgccccc
    68701 gtttcttgtc acaataggcc tttgcgcgca tcgatacgtt gcgtgaggta cacagtcatc
    68761 tacagcggag tgttgttaca ccatgatgga cagcttacgc acggctgcaa acagtctcgt
    68821 gctcaagatt attttcggta tcattatcgt gtcgttcata ttgaccggcg tgagtggtta
    68881 cctgattggc ggaggcaata actacgccgc aaaagtgaat gaccaggaaa tcagccgtgg
    68941 gcagttcgag aacgccttta acagcgagcg taatcgcatg cagcaacagc tgggcgatca
    69001 atactccgag ctggcagcga atgaaggcta tatgaaaacc ctgcgtcaac aggtgctgaa
    69061 tcgtctgatc gacgaggcgc tgttggatca gtacgctcgt gagctgaaac tgggtatcag
    69121 tgatgagcag gttaaacagg cgattttcgc gaccccagcc ttccaggttg acggcaaatt
    69181 tgataacagc cgctataacg gtatcctcaa ccagatgggg atgaccgccg atcagtacgc
    69241 ccaggcgctg cgtaaccagc tcactaccca acagctgatt aacggtgttg ccggtaccga
    69301 ttttatgctg aaaggtgaaa ccgacgagct ggcggcactg gtcgcgcaac aacgcgtggt
    69361 gcgtgaggcg actatcgatg ttaacgcgct ggcggcgaag cagcctgtga ccgaacagga
    69421 gattgccagc tactacgaac aaaacaaaaa caatttcatg acgccggaac aattccgcgt
    69481 gagttacatc aagctggatg ccgcaacgat gcagcaaccg gttagcgatg cggatatcca
    69541 gagctactac gatcagcatc aggatcaatt cacccagccg cagcgtaccc gctacagcat
    69601 catccagacc aaaactgaag atgaagcgaa agcggtactt gatgagctga ataaaggcgg
    69661 tgattttgct gcattagcca aagaaaaatc tgccgatatt atctctgccc gtaacggcgg
    69721 cgatatgggt tggttagaag atgccactat ccccgacgaa ctgaaaaatg ctggtctgaa
    69781 agaaaaaggc caactgtctg gtgtcatcaa atcttcggtc ggtttcctga ttgtacgtct
    69841 ggacgacatt cagccagcga aagtgaaatc gttagacgaa gtacgtgacg acattgcggc
    69901 gaaagtgaaa cacgaaaaag ccctcgatgc gtaccatgcg ctgcagcaga aagtgagcga
    69961 tgcggcaagc aacgacaccg agtctctggc cggtgcagag caagctgccg gcgttaaagc
    70021 cactcagacg ggttggttca gcaaagataa cctgccggaa gagttgaact tcaagccggt
    70081 tgccgacgct atctttaacg gcggtctggt aggtgaaaac ggcgcgccgg gcatcaactc
    70141 tgacatcatc accgtagacg gcgaccgtgc attcgtgctg cgcatcagcg agcacaaacc
    70201 ggaagcggtg aaaccgttgg cagatgttca ggaacaagtt aaggcactgg ttcagcacaa
    70261 caaagctgaa caacaggcga aagtggatgc tgagaaactg ctggttgatt tgaaagccgg
    70321 caaaggtgcg gaagctatgc aggctgccgg tctgaaattt ggcgagccga aaaccttaag
    70381 ccgttccggt cgtgacccga ttagccaggc ggcgtttgca ctgccactgc cagcgaaaga
    70441 caaaccgagc tacggtatgg cgaccgatat gcaaggtaat gtggttctgc tggcgctgga
    70501 tgaagtgaaa caaggttcaa tgccggaaga tcagaaaaaa gcgatggtgc agggtatcac
    70561 ccagaacaac gcacaaatcg tctttgaagc tctgatgagt aacctgcgta aagaggcgaa
    70621 aatcaaaatt ggcgatgcgc tggaacagca ataatcctga agccgcctcg caaaaaaatg
    70681 cgttgcagct gtaacaagaa aaggtcgctt tcgcggcctt ttccatttct gaacattgcc
    70741 atttgtttac tgttttcact gccgttaagg tgattccact gttaacaaac aaggagaaaa
    70801 cagtatgaaa cacggaatta aagcactgct cattaccctg tccctggcct gtgccggaat
    70861 gtctcatagc gcgctggcgg cagcttctgt ggcgaaaccg acggcggtag aaaccaaagc
    70921 ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg acgaagaagg
    70981 cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga tgaatggcgt
    71041 tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc cgtttaaaac
    71101 agtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac gtaatctggc
    71161 ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg aagaggtcat
    71221 accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta tgcaaacaca
    71281 aataaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg cccgctacct
    71341 tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt ttcagtggat
    71401 gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc gtcgcccagc
    71461 ggtattaagt gacctgttaa ctattaccag tcagttgcag caattaaacg gtaaaagcgg
    71521 catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg atgcgcttat
    71581 tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag gggaattgcg
    71641 cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct caagccgcat
    71701 ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct gccatcaccg
    71761 tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat gccgcacaat
    71821 gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta cggactaaat
    71881 ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc cacatcagcg
    71941 gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt agtgctttca
    72001 caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg ccggtaatta
    72061 cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac aaaatattac
    72121 gccctgggac aaacgtattc gggatgccat cggcttcagg ttcataatca ggcaccggaa
    72181 tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca tcaagaacct
    72241 tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt tctgcgcgat
    72301 gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt aatgcctgca
    72361 ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt ttcatagata
    72421 atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg accagctatt
    72481 cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg cggtgtcagt
    72541 cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa cagcggcgtc
    72601 agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa tcggttttcc
    72661 tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata tgctggagcg
    72721 tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt atattccggt
    72781 gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag ggtcgtgtcg
    72841 tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc tgccagtgtc
    72901 gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac cagcgtcaag
    72961 gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca gcctggcagt
    73021 aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa taaaccggat
    73081 tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg acttttgcgc
    73141 aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac ccgttgcagc
    73201 tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc caaatctttt
    73261 tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg acgcaaatgg
    73321 taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa gcgaaaagga
    73381 ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct cgccagccgg
    73441 tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg atgggtgact
    73501 tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa cagcatcaat
    73561 aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg tagggttgaa
    73621 cgaagataaa agtcccagcg taacccgtca gtagaggttt cccagtgatg cgctaaatcg
    73681 ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat ctgcccggcg
    73741 agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag cgggcgatag
    73801 tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa cggctgtaac
    73861 agagtgcgta gctcacctgg ggccagttgc gccagctcca gcacatcttg ctgctttccg
    73921 gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac cagaaagcgt
    73981 aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc cgcctcctgt
    74041 gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc ggccagttcg
    74101 ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg atactggtta
    74161 agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt cactattact
    74221 tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg gagaaagaaa
    74281 tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac catcatttag
    74341 gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc ctcacttttg
    74401 ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg ctggatgcat
    74461 atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt ttacatcgtg
    74521 atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat acccgagcca
    74581 gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct gcgttgttgc
    74641 aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa atgccactcg
    74701 gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc ttgcagatcc
    74761 agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccacg gattgcctcg
    74821 aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc caacatctag
    74881 gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc gaaatgttag
    74941 gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc gcggagctcc
    75001 cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat ttgacgcact
    75061 gactggacta tccacatcta ccttattccc ccgaataatg agatcccttc cagcaccggg
    75121 caattgcccg gttttttttg cgttgaattt gtcattttgt gccgtggtgt ttaaaccgca
    75181 cagaataaat tgtcgtgatt tcacctttaa aatagaatta aaagagaaaa aaattctctg
    75241 tggaagggct atgttagata aaattgaccg taagctgctg gccttactgc agcaggattg
    75301 caccctctct ttgcaggcac tggctgaagc cgttaatctg acaaccaccc cttgctggaa
    75361 gcgcctgaaa cggctggagg acgacggtat ccttatcggc aaagtcgccc tgctggatcc
    75421 ggaaaaaata ggcctcggcc tgaccgcctt tgtgctgata aaaacgcaac atcacagtag
    75481 cgaatggtat tgccgctttg tcacggtggt taccgaaatg ccagaagtgc tggggttctg
    75541 gcgcatggct ggtgaatacg attatctgat gcgcgtccag gttgccgaca tgaaacgcta
    75601 cgacgagttt tataagcgtc tggtaaacag cgtgccgggg ctgtcggacg tcacttccag
    75661 cttcgcgatg gaacagatta aatacaccac ttctttaccc atcgaataaa tatccagaat
    75721 caggtcagga cacaacgcgt gcgattattt gctcaattaa gctggtattt ccgtcgggaa
    75781 tggcgtcgct atctcggggc tgtcgccttg cttgtcatta tcgcgatgct gcaactggtt
    75841 ccgccaaaag tggttggtat tgttgtcgat ggcgtgacag aacaacactt tactaccggg
    75901 cagatcctga tgtggatcgc caccatggtg ctgattgccg ttgtggttta tttcctgcgt
    75961 tacgtctggc gggtattgct gtttggtgcg tcttatcaac tggctgttga actgcgtgaa
    76021 gattattacc gtcagctaag ccggcagcat cctgagtttt acctgcgtca tcgcacgggt
    76081 gacctcatgg ctcgtgcgac caatgacgtc gatcgcgtcg tgtttgcagc cggagaaggg
    76141 gtgctgacgc tggtggattc actggtgatg ggctgcgctg tgttgattat gatgtctacg
    76201 caaattagct ggcagttgac cttatttgcc ctgttgccga tgccagtgat ggcgatcatg
    76261 atcaagcgca acggcgatgc cttgcatgaa cgctttaagc tggcacaggc ggcgttttct
    76321 agtcttaatg accgcaccca ggaaagcctc accagtatcc gcatgatcaa agcctttggt
    76381 ctggaagatc gccagtcggc gttatttgcc gcggatgccg aagataccgg caaaaaaaac
    76441 atgcgggtgg cgcgtattga tgctcgtttc gacccgacca tctatatcgc gattggtatg
    76501 gcgaacttgc tggcgattgg cggcggtagt tggatggtgg tgcagggcag tttaacgctg
    76561 ggccagctca ccagttttat gatgtattta ggtctgatga tttggccaat gctggcgctg
    76621 gcatggatgt ttaacattgt ggaacgtggt agtgctgcat acagccgtat tcgcgcgatg
    76681 ctggcggaag cgccggtggt gaacgatggt agcgaacccg tgccggaagg gcgtggcgaa
    76741 ctggatgtaa atattcacca gttcacgtat ccgcagactg accatcctgc gctggaaaac
    76801 gtcaatttcg ccctgaaacc cggtcagatg ctgggtatct gcgggccgac tggttccggc
    76861 aaaagtaccc tgttgtcgct cattcagcgt catttcgacg tcagcgaggg ggatattcgc
    76921 tttcatgata ttcctctgac gaagttacaa ctcgatagct ggcgtagccg cctggcggta
    76981 gttagccaga caccgttcct tttttccgac accgtagcga acaacatcgc gttgggttgc
    77041 ccgaatgcca cccagcaaga gattgagcat gtcgcgcggt tagccagcgt acatgacgat
    77101 attttgcgtc taccgcaagg ttacgataca gaagtgggcg agcgcggcgt gatgctttcc
    77161 ggtgggcaaa aacagcgtat ctccattgct cgagcgttat tagtcaacgc ggaaatcctc
    77221 atccttgatg atgcgctttc ggcggtggac ggacgcacag agcaccagat cctgcataac
    77281 ctgcgtcagt ggggacaggg aagaacggta atcatcagtg cccatcgcct ttcggcactg
    77341 acggaagcca gt
//
LOCUS       DADLTD010000002        49519 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLTD010000002 DADLTD010000000
VERSION     DADLTD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.002"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="JN701_000078"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="JN701_000078"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY7362635.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JN701_000079"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JN701_000079"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY7362636.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKHAPRREGTA"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JN701_000080"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JN701_000080"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414686.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY7362637.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /locus_tag="JN701_000081"
     CDS             complement(3024..3479)
                     /locus_tag="JN701_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY7362638.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JN701_000082"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JN701_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY7362639.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JN701_000083"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JN701_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY7362640.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFLPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="JN701_000084"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="JN701_000084"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY7362641.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10128
                     /gene="mrcB"
                     /locus_tag="JN701_000085"
     CDS             7606..10128
                     /gene="mrcB"
                     /locus_tag="JN701_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY7362642.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10348..12537
                     /gene="fhuA"
                     /locus_tag="JN701_000086"
     CDS             10348..12537
                     /gene="fhuA"
                     /locus_tag="JN701_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY7362643.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12588..13385
                     /gene="fhuC"
                     /locus_tag="JN701_000087"
     CDS             12588..13385
                     /gene="fhuC"
                     /locus_tag="JN701_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY7362644.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13385..14275
                     /gene="fhuD"
                     /locus_tag="JN701_000088"
     CDS             13385..14275
                     /gene="fhuD"
                     /locus_tag="JN701_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY7362645.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14272..16254
                     /gene="fhuB"
                     /locus_tag="JN701_000089"
     CDS             14272..16254
                     /gene="fhuB"
                     /locus_tag="JN701_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY7362646.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16289..17569)
                     /gene="hemL"
                     /locus_tag="JN701_000090"
     CDS             complement(16289..17569)
                     /gene="hemL"
                     /locus_tag="JN701_000090"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY7362647.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17794..19215
                     /gene="clcA"
                     /locus_tag="JN701_000091"
     CDS             17794..19215
                     /gene="clcA"
                     /locus_tag="JN701_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY7362648.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMEALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19239..19304
                     /locus_tag="JN701_000092"
     CDS             19239..19304
                     /locus_tag="JN701_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362649.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19297..19641
                     /gene="erpA"
                     /locus_tag="JN701_000093"
     CDS             19297..19641
                     /gene="erpA"
                     /locus_tag="JN701_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY7362650.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19688..20311)
                     /locus_tag="JN701_000094"
     CDS             complement(19688..20311)
                     /locus_tag="JN701_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY7362651.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20354..21148)
                     /gene="btuF"
                     /locus_tag="JN701_000095"
     CDS             complement(20354..21148)
                     /gene="btuF"
                     /locus_tag="JN701_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY7362652.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASPGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFDVQAHVLSSLHNSSVMRFHR"
     gene            complement(21141..21839)
                     /gene="mtnN"
                     /locus_tag="JN701_000096"
     CDS             complement(21141..21839)
                     /gene="mtnN"
                     /locus_tag="JN701_000096"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY7362653.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHGKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21923..23440
                     /gene="dgt"
                     /locus_tag="JN701_000097"
     CDS             21923..23440
                     /gene="dgt"
                     /locus_tag="JN701_000097"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7362654.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNIAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23570..24994
                     /gene="degP"
                     /locus_tag="JN701_000098"
     CDS             23570..24994
                     /gene="degP"
                     /locus_tag="JN701_000098"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY7362655.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25149..26306
                     /locus_tag="JN701_000099"
     CDS             25149..26306
                     /locus_tag="JN701_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HAY7362656.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26395..26781)
                     /locus_tag="JN701_000100"
     CDS             complement(26395..26781)
                     /locus_tag="JN701_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY7362657.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26943..27755)
                     /gene="yaeI"
                     /locus_tag="JN701_000101"
     CDS             complement(26943..27755)
                     /gene="yaeI"
                     /locus_tag="JN701_000101"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HAY7362658.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27810..28634)
                     /gene="dapD"
                     /locus_tag="JN701_000102"
     CDS             complement(27810..28634)
                     /gene="dapD"
                     /locus_tag="JN701_000102"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY7362659.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28665..31337)
                     /gene="glnD"
                     /locus_tag="JN701_000103"
     CDS             complement(28665..31337)
                     /gene="glnD"
                     /locus_tag="JN701_000103"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY7362660.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31399..32193)
                     /gene="map"
                     /locus_tag="JN701_000104"
     CDS             complement(31399..32193)
                     /gene="map"
                     /locus_tag="JN701_000104"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY7362661.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32561..33286
                     /gene="rpsB"
                     /locus_tag="JN701_000105"
     CDS             32561..33286
                     /gene="rpsB"
                     /locus_tag="JN701_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY7362662.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33544..34395
                     /gene="tsf"
                     /locus_tag="JN701_000106"
     CDS             33544..34395
                     /gene="tsf"
                     /locus_tag="JN701_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY7362663.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34542..35267
                     /gene="pyrH"
                     /locus_tag="JN701_000107"
     CDS             34542..35267
                     /gene="pyrH"
                     /locus_tag="JN701_000107"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY7362664.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35559..36116
                     /gene="frr"
                     /locus_tag="JN701_000108"
     CDS             35559..36116
                     /gene="frr"
                     /locus_tag="JN701_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY7362665.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36208..37404
                     /gene="ispC"
                     /locus_tag="JN701_000109"
     CDS             36208..37404
                     /gene="ispC"
                     /locus_tag="JN701_000109"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY7362666.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAAYDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37592..38350
                     /gene="ispU"
                     /locus_tag="JN701_000110"
                     /gene_synonym="uppS"
     CDS             37592..38350
                     /gene="ispU"
                     /locus_tag="JN701_000110"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY7362667.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38363..39220
                     /gene="cdsA"
                     /locus_tag="JN701_000111"
     CDS             38363..39220
                     /gene="cdsA"
                     /locus_tag="JN701_000111"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7362668.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39232..40584
                     /gene="rseP"
                     /locus_tag="JN701_000112"
     CDS             39232..40584
                     /gene="rseP"
                     /locus_tag="JN701_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY7362669.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40614..43046
                     /gene="bamA"
                     /locus_tag="JN701_000113"
     CDS             40614..43046
                     /gene="bamA"
                     /locus_tag="JN701_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY7362670.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43168..43653
                     /gene="skp"
                     /locus_tag="JN701_000114"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43168..43653
                     /gene="skp"
                     /locus_tag="JN701_000114"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY7362671.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43657..44682
                     /gene="lpxD"
                     /locus_tag="JN701_000115"
     CDS             43657..44682
                     /gene="lpxD"
                     /locus_tag="JN701_000115"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY7362672.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44787..45242
                     /gene="fabZ"
                     /locus_tag="JN701_000116"
     CDS             44787..45242
                     /gene="fabZ"
                     /locus_tag="JN701_000116"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY7362673.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45246..46034
                     /gene="lpxA"
                     /locus_tag="JN701_000117"
     CDS             45246..46034
                     /gene="lpxA"
                     /locus_tag="JN701_000117"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY7362674.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            46034..47182
                     /gene="lpxB"
                     /locus_tag="JN701_000118"
     CDS             46034..47182
                     /gene="lpxB"
                     /locus_tag="JN701_000118"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY7362675.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47179..47775
                     /gene="rnhB"
                     /locus_tag="JN701_000119"
     CDS             47179..47775
                     /gene="rnhB"
                     /locus_tag="JN701_000119"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY7362676.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMVVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47812..>49519
                     /gene="dnaE"
                     /locus_tag="JN701_000120"
     CDS             47812..>49519
                     /gene="dnaE"
                     /locus_tag="JN701_000120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7362677.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYYDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WAL"
BASE COUNT        11655 a        12198 c        13677 g        11989 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aattcgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gatcctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgagcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtggag
      481 tgcggtaaaa cgaagaaacg gcaagaatgt ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgcg gtaccctcac gacgcggtgc gtgtttgcgt ggacgacgag tacggcgacg
      661 cggtgacggc tcttcatcca actcgttaag catccctttt tgatctggtg gcgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcgttacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc atgccgccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggttg caacttcgat aatctccggg ccaaacatca catgagccag
     1681 acggaaacgg cgacccacaa ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttattgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag cgtccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctacctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg agatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgccgcgc
     2521 agctggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgacaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc
     3841 gcacgctggc cttcagccgc tacgttcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttctgcg
     4021 ccatatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttagttc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc acgcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaagggga gagaattcca ttgcgacaat ccttgttatt
     4381 gcattagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acctggcggc gggattgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gcggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctcggcgct cacttcgcct aaaaatgcca gagtcagatg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtagg aagtgtgtgg cgcgccagtg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgttttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggaggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggccgaatac ccgcctggaa gtagttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggagtt gagcggtgtt ggattggtga
     5341 tccttgatga atttcatgag cgtagcttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctaat tatgtcggct acgctggaca
     5461 acgaccgctt gcagcaaatg ctgccagaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gatgccgttg
     5581 cggtagccac cgctgaaatg ctgcgtcagg aaagcggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgt gtgcaggaac aactggcttc gcgcatcggc agtgatgtat
     5701 tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgt tttgatccgc
     5881 gcacggggct aacgcgactg attactcaac gcgttagcca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tctgcctgca tctaatcgcc aaagaacaag
     6001 cagaacgcgc tgccgcgcaa agtgaaccgg aaatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcag gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttagcgcg aagaacgacg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgtggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcgtcgccg tgggcaagat ggacgctatc aactggcaaa cggcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctgccggt cgatattgat gagttagtac
     6661 aacgctgccc gcagctggta cagcagtctg acactgtgga gtgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctgcaaatcg gtctgctgac ggtgaaagtg cagccgctgg
     6781 cgaaaccctc ggaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagttta ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgactg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaagagat gtttggcgag gccaccaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctggagtt gctttcacct gcccaaagtc
     7261 cattacaaat cacacgagat ttgagcgcct tctggaaagg agcgtaccgt gaggtgcaaa
     7321 aagagatgaa agggcgttat cccaaacatg tctggccgga cgacccggca aatactgcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccgggatg agggcatcag cgtgcgcatt tccctctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgaaca caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcctgcggca
     7921 gtttatggcc gaatggtcaa tcttgagcca gatatgacca tcagcaagaa cgagatggtg
     7981 aagctgctgg aggcgactca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgca tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggctttttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca acgtctgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgc catttctacg agcatgatgg gatcagcctc
     8341 tactcaattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ctcttactgg
     8461 cgtaaagcga acgaagccta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgcggt
     8821 ggggtgatct ctcctcagcc agcctttatg caactggtgc gtcaggagct gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtgg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 ctggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacccgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcag ttacggatac ctggatcaaa ctgggcgtac cgaaagatca gctgcatccg
     9421 gttccggcga tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcgttc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggca aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatactt ggtttgcagg cattgacggc agcacggtga ccatcacctg ggcaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcaa cagcaacctg ctcagcaaga gcagaaagac
    10081 agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca tctaagcgtg
    10141 aaataccgga tggcgagttg ccatccggta aaataacatc ccatctaaga tattaaccct
    10201 ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt accgcttgcg
    10261 aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg tttacgttat
    10321 cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac
    10381 tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca
    10441 caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa
    10501 gaaagcgcat gggggcctgc tgcaactatt gcggcgaagc actctgctac tgcgactaaa
    10561 acggatacac caattgaaaa aacgccgcag tctatttcgg ttgtgacaaa tgaagagatg
    10621 cagatgcatc aatttcagtc tgtaaaagaa gcattaggtt atacacctgg cgttacggtt
    10681 agcagtcgtg gtgcttctaa tacatatgat tttgttatca ttcgtggttt ctcatctgtt
    10741 ggtctgaatc aaaataatta ccttgatgga ctaaaacttc agggcaactt ttataacgat
    10801 gctgtgattg atccttacat gctcgagagg gttgaactga tgcgtgggcc gacgtctgtt
    10861 ctctatggaa aaagtaatcc tggtgggatt atttctatgg tgagtaagcg tccgaccact
    10921 gaacccctga aagaaattca gtttaaaatg gggacggata atctgtttca gaccggattc
    10981 gatttcagtg atgcactgga tgataacggt gagttctctt accgtttgac gggcctcgct
    11041 cgctcaacaa acgaacagca gaaaaactct gaatctcagc gttataccat tgctccatca
    11101 ttctcatggc gtccagacga caaaaccaat tttactttcc tgtcctattt ccagaatgaa
    11161 ccagaaacgg gttattacgg ttggttgccg aaagagggga ccgttgagcc attgcctaat
    11221 ggtaagcgtc taccgactga cttcaatgaa ggtgcatcga ataatacata ctcccgtaac
    11281 cagaaaatgg tgggatatag ttttgaacat ggttttaatg acaccttcac cgtgcgtcag
    11341 aatctgcgtt tcagtgaaat gaaaacctca cagaaaagtg tttatggcac agggattgct
    11401 ggtgatggtc ataccctaaa ccgcgggaca gtggtggata atgagcgtct acaaaacttt
    11461 agcgttgata cccaacttga aagtaagttt gctacaggtg acgtcgagca tactttgctg
    11521 acaggggtag acttcatgcg gatgcgtaat gatatcaatg ccagctttgg atccgcacca
    11581 tccatcgatc tttataacaa atatcaccct gaatactttg catttggtag cgcagagcca
    11641 taccagatga atgaaagcaa acaaacaggt atttatgttc aggatcaggc ggaatggaat
    11701 aaatgggtat tcactctggg gggacgctac gattggtcta agcaagcgac cactgttcgt
    11761 gaaaactctt atacgccgac tgaaggttat attgagcgca atgatcatca gttcacctgg
    11821 cgcggtggtg taaattactt attcgataat ggtatttcac cttactttag ctatagccag
    11881 tcctttgaac cgagtgcttt cgatctgtgg agcaacccac gcatttccta taagccatcg
    11941 aaaggtgaac agtatgaagc tggcgtaaaa tatgttccga atgatatgcc ggtcgttgtt
    12001 acgggcgcag tctatcaatt gacgaaaaca aataacctga cagcagaccc aacaaaccca
    12061 ttagcgcaag tcccagcagg tgagattcgc gctcgtggtg tggaacttga agcaaaagcg
    12121 gcgttaaatg ccaatattaa cttgacggct tcttatacct acaccgatgc ggaatacacc
    12181 aaagacacca atctcaaagg taaaactcca gaacaagtac cggagcatat ggcatctctc
    12241 tggggggatt ataccttcaa tgaagggccg ctttctggtt taacattggg aacaggtggt
    12301 cgttttattg gttccagcta tggtgatccg gcaaacactt ttaaagtggg tagcgcagct
    12361 gtaatggatg ctgttgtaaa atatgatctg gcacgctttg gtatggcggg atccagcctt
    12421 gctgtgaacg tcaacaattt gctcgatcgt gagtatgttg ccagttgctt ccagacttat
    12481 ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg caaccttccg tttctaattt
    12541 ctcttttggg gcacggattt ccgtgcccat ttcacaagtt ggctgttatg caggaataca
    12601 cgaatcattc cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca
    12661 cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa agtgaccggt ctgattggtc
    12721 acaacggttc tagtaaatcc actctgctca aaatgcttgg ccgtcatcag cctccgtcag
    12781 aaggggagat tcttcttgat gcccagccgc tggaaagctg gagcagcaaa gcgtttgccc
    12841 gcaaagtggc ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac
    12901 tggtggcgat tggtcgttac ccgtggcatg gcgcgctggg gcgctttggg gcggcagatc
    12961 gcgaaaaagt cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg
    13021 ttgatagtct ctctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg
    13081 atagccgttg tctgttgctc gacgaaccga cctcggcgct ggatatcgcc caccaggttg
    13141 atgtgctggc gctggtgcac cgtttaagtc aggagcgtgg cctgacggtc attgccgtgt
    13201 tgcacgatat caatatggcg gcacgctact gtgattatct ggtcgccctg cgcggtggtg
    13261 aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg cgataccctc gaaatgattt
    13321 atggcatccc gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt
    13381 attgatgagc ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc
    13441 tccgttgtta tggcagatga ataccgccca cgcggcggct attgatccca atcgtattgt
    13501 ggcgctggag tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttacggcgt
    13561 agcggatacc atcaactatc gcctgtgggt cagcgaacca ccattgccgg actcagtgat
    13621 cgacgtcggt ttgcgcacag aacctaacct tgaactgctg accgaaatga aaccgtcgtt
    13681 tatggtctgg tcggcaggat atggcccttc agcagaaatg ctggcacgta ttgcgccggg
    13741 gcgtggattt aacttcagcg acggcaaaca gccgctggcg atggcgcgta aatcactgac
    13801 ggaaatggca gatttactta acctgcaaag cgcagcagaa acgcatttag cgcaatatga
    13861 agactttatc cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttattgct
    13921 gacgacgctt atcgatccgc gccatatgtt ggtcttcggt ccaaacagct tgttccagga
    13981 aattcttgat gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag
    14041 taccgccgtc agtatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga
    14101 tcacgacaac agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc
    14161 gtttgtccgc gccggacgct ttcagcgcgt acccgcagtc tggttttatg gtgcgacgct
    14221 ctcggcaatg cactttgtgc gcgttctgga taacgccatc ggaggtaaag cgtgagtaaa
    14281 cgaattgcgc ttttcccggc gttattgctg gcgctgctgg tgattgtcgc tacggcgctg
    14341 acctggatga acttttcgca ggcgctgccg cgtagccagt gggcgcaggc cgcctggtcg
    14401 ccggatattg acgtcatcga gcagatgatt tttcactaca gcttgctgcc gcgtctggcg
    14461 atttcgctgc tggtgggcgc aggcctgggg ctggtgggcg tgctgtttca gcaagtgctg
    14521 cgtaatccgc tggcggaacc gacgacactt ggcgttgcta caggcgcgca actggggatt
    14581 accgtcacta cgctctgggc gatcccaggg gcgatggcga gtcagtttgc tgcgctggcg
    14641 ggcgcttgcg ttgttggctt aatcgtcttt ggcgtcgcgt gggggaaacg gctttcgccg
    14701 gtaacgctga tcctcgcggg gctggtagtg agcctttatt gtggggcaat caatcagtta
    14761 ctggttatct tccaccatga ccaactgcaa agcatgttcc tgtggagcac cggaacgctg
    14821 acgcaaaccg actggggcgg tgttgagcgt ttatggccgc agttgctggg cggtgtgatg
    14881 ctgacgttgc tgctacttcg cccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc
    14941 aatctcgggc tggccttgtc gctcgcgcgt ctggcggcgc tgtcgctggc gattgtcatc
    15001 agtgcgctgc tggtgaacgc ggtggggatt atcggcttta tcgggttgtt tgcgccgttg
    15061 ctggcaaaaa tgctgggggc gcggcgtctg ctgccgcgac tgatgctggc gtcgttgatt
    15121 ggtgcgctga tcctctggct ttccgatcaa atcatcctct ggctgactcg cgtgtggatg
    15181 gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgttg
    15241 ccgcgtttac gcagcattag cgcgccggat atgaaggtca acgatcgtgt cgcggctgaa
    15301 cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggtg
    15361 gtggcgctgt cgtttggtcg tgatgcgcac ggctggatgt gggcgagcgg ggcgttgctc
    15421 gatgatttaa tgccctggcg ctggccgcga attatggcgg cgctatttgc gggcgtcatg
    15481 ctggcggtgg cgggctgtat tattcagcga ctgaccggca acccgatggc aagcccggaa
    15541 gtgctgggga ttagctccgg cgcagcgttt ggcgtggtac tgatgctgtt tctggtaccg
    15601 ggtaatgctt ttggctggct gttacccgcg ggaagtatcg gtgccgcggt gacgctgttg
    15661 attattatga tcgccgccgg gcgcggtgga ttttccccac accgcatgtt actggcgggg
    15721 atggcgttaa gtaccgcatt caccatgctt ttaatgatgt tgcaggcgag tggtgacccg
    15781 cgaatggcgc aagtgctcac ctggatctct ggttcgacct acaacgccac cgatgcgcag
    15841 gtctggcgta ccggaattgt gatggtgatt ttgctggcga ttaccccgct gtaccgccgc
    15901 tggctgacca ttttacctct gggcggtgat accgcacgag ctgtgggaat ggcgctgacg
    15961 ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga cggcgacctc gacgatgact
    16021 attggaccgt tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggtttt
    16081 cgacggacga tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtattc
    16141 gctgactggt gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtcg
    16201 acctttatcg gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaattgagt
    16261 gcctcgcccg gtgggcgagg caaaattatc acaacttcgc aaatacccga cgtgcagcat
    16321 cgatggtgtt attgatatct tccatgctgt gcgccacaga cataaagccc gcttcaaacg
    16381 ctgacggtgc caggtaaaca ccttcgtcca gcatcatatg gaagaaacgc ttaaagcgtt
    16441 ccacgtcaca ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaaa
    16501 taccgaacat gccgccaacg tggttaacga ccagcggagt tccggcttct tctgccgctt
    16561 ccagcagacc ttctgccaga cgtgttgtca gctcatccag cgtttcgtga acgcccggct
    16621 gcgcgacttc attcagacag gcgaaacccg ctgccatcgc aatcgggtta ccggaaagcg
    16681 tacccgcctg atagaccgga cccgtcgggg ccagcgcatc cattacatca cgacgaccac
    16741 cgaatgcgcc taccggcatt ccaccgccga tgattttgcc gaggcaggtt aaatctggca
    16801 ctacgccgta ataatcctgt gcgccagcca gtgccacgcg gaagccggtc atcacttcat
    16861 cgatgatcag caacgcgcca aattcgtcgc acagcgcacg cagacctggc aggaactctg
    16921 gctgcggcgg aacacagttc atattgcctg ccaccggctc gacgataata caggcaatct
    16981 cttgcgggta ttgctcaaac gcggcgcgta cagaagccag atcgttataa gtacaggtta
    17041 aggtatgttt ggcgaaatct gccggaacac ccggcgagtt tggctggcct aacgtgagtg
    17101 cgccagaacc ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt
    17161 taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atggtcgctt
    17221 cagtgccgga gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt
    17281 gcgccatttt cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt
    17341 caatcacggc attgcggata gccggatggt tatggcccag caccatcggc ccccaggaac
    17401 cgacataatc gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt
    17461 cgataaacag tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc
    17521 cagggagcag ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct
    17581 gattcgtaga aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg
    17641 tcaaacatca ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt
    17701 gcatttgaaa cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc
    17761 atcttatttg tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa
    17821 acaccgcagg ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat
    17881 aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggca
    17941 gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatgga ggcgctggta
    18001 catactgctg ataattatcc gcttctgtta accgtcgctt ttctctgttc ggcggtgctg
    18061 gcgatgttcg gctacttttt ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc
    18121 ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc gctggtggcg tgtgttgccg
    18181 gtgaaattct ttggcgggct ggggacactc ggcggaggca tggtgctggg gcgcgaaggg
    18241 ccaaccgtgc agatcggcgg caatatcggt cgcatggtac tcgatatttt ccgcctgaaa
    18301 ggtgatgaag ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc
    18361 tttaacgcgc cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc
    18421 tatacgttaa tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac
    18481 cggattttta atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt
    18541 aatacgctgt ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt
    18601 aataaatggg tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc
    18661 aaatgggtgc taatgggcgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca
    18721 ccagcaacgt cgggcggcgg ttttaacctg attcctatcg ctaccgcagg gaatttcagc
    18781 atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta ccaccttact gtgcttctct
    18841 tccggcgcgc cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc
    18901 gctttcggaa tggttgtcgt tgagctgttt ccgcaatatc accttgaggc ggggactttt
    18961 gctattgccg gaatgggggc gttactggcg gcatctattc gcgcgccgtt aacggggatc
    19021 attttggttc tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt
    19081 cttggcgcaa cactattagc gcaatttacc ggcgggaaac cgctatattc ggagattctt
    19141 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc
    19201 agcgagaata cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag
    19261 aatttgccca attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg
    19321 ctggagttta ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac
    19381 ccgaatctga aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt
    19441 ttcacctttg atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc
    19501 ctggtggttg atccgatgag cctgcaatat ctggtcggtg gttccgttga ctataccgaa
    19561 ggactggaag gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc
    19621 ggttcttcct ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct
    19681 tctctcttca acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa
    19741 tagccgccag ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg
    19801 gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg
    19861 cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc cagtacatca cgaatgatcc
    19921 cgccgccaac gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat
    19981 taaaggcttt attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg
    20041 gcaacatcca ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg
    20101 ttaccattgc aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca
    20161 gcgccatgtc gcgaattgtc ccgccgccta cagcggtaac cacgcccagt accagaacac
    20221 caaaagggtc catacgcaat tttccggcta acaaaacgcc ggagatggca aataccgctg
    20281 tgccgactat atccagccaa tagacgagca ttgttcaatc cccactgagc acctaaaaag
    20341 gtcaggcgct aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata
    20401 cgtgggcttg cacgtcaaac cagtcactcg tgagaggaat aacgggaatt ttgagctgtt
    20461 caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg acaatcgcct
    20521 gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccaaggaacc cggctgtctt
    20581 taaagatgtt ttctccgcca caaacttcga gtacctggtt ttgaatcgac tcttttccac
    20641 tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt ttatcagcat
    20701 attgcgcttt caattgcgcg tactgatcca gcagggattg cgcggcttgt tcggccttat
    20761 ccggttgcgg actccagggg gccagttgac gtaacgcatt ggcaatttgt tcaatgcttg
    20821 tcgcatcgac ccacatcact tttattcccg gcgaagccag ctggtcaacc tgccgctcgg
    20881 cattacctcc acgccaggca atcaccagat cgggtttcag cgcgacaatg cgttccagat
    20941 tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc ttgtggagga tagtcggaat
    21001 agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg ttggcgggag
    21061 aaagcgtgat gacgcgcggt gccgcgttga gccacagtgg cgcgagaaaa gacagggcga
    21121 ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga ctcaaccatc
    21181 aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg agactgttga
    21241 tcggccacgt cggagatggc gcgtacgaca acaaacggga cgttgaaatt gtggcagaca
    21301 tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt gtggcggatt
    21361 ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc taacaatcag gccacgtaca
    21421 gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt atcgtcagct
    21481 ttaaagcctg ccggacagcc tggtaactga ccgtattcat aaccaaatgc cgtgacatcc
    21541 gcgtcgtgat aacgtgcttc gtccgagaca acgatatcgc ccactttcaa cgttggtgcc
    21601 aggccaccgg cagaaccggt gttaataatc acatctggct tgccgtgttc caacagcaaa
    21661 gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc aacctcggtt
    21721 ccattcagtt ggccggtata gatttcgcaa ccgccgagac tgatagtttg acgattttcg
    21781 attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc gattttcata
    21841 gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta cggggaagcg
    21901 tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa taaactggca
    21961 tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagatcctgc ggatcttcga
    22021 gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac aaaagaccca
    22081 ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact cgatggaagt
    22141 ccagcaggtg gggcgctaca tcgccaaaga aattctaagc cgtatgaaag agcttaaatt
    22201 actggaagca tacggcctgg atgaactgac cggccccttt gaaagcattg ttgagatgtc
    22261 atgcctgatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag cggcgataaa
    22321 tgattggttt cgccagcgtt tgcacccgga agatgccgaa agccagcctc tgactgacga
    22381 tcgctgcagc gtggcggcac tacgtttacg ggacggggaa gaaccgctta acgagctgcg
    22441 gcgcaagatt cgtcaggact tatgtcattt tgaggggaat gcacaaggca ttcgtctggt
    22501 gcatacattg atgaggatga atctcacctg ggcacaggtt ggcggtattt taaaatatac
    22561 ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat cactatttaa tgaaaaagcc
    22621 gggttattat ctttctgaag aagcctatat tgcccggttg cgtaaagaac ttaatttggc
    22681 gctttacagt cgttttccat taacgtggat tatggaagca gccgacgaca tctcctattg
    22741 tgtggcagac cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca
    22801 tcatttgcac gaagcgtggg gccagcatga gaaaggttcg ctcttttcgc tggtggttga
    22861 aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt
    22921 tatgtattta cgggtaaaca ccctaaataa actggtaccc tatgcggcac aacgatttat
    22981 tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag
    23041 cgaatgcagc gatcttctta agctatataa aaatatcgct gtaaaacatg tgtttagcca
    23101 tccagatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat
    23161 ttatcgtcct ttattaagcc tgtcgttatc cgactttact gaactggtag aaaaagaacg
    23221 ggtgaaacgt ttccctattg aatcgcgctt attccacaaa ctctcgacgc gccatcggct
    23281 ggcctatgtc gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga
    23341 atattattac cgttgccgcc tgctgcagga ttatatcagc ggtatgaccg acctctatgc
    23401 gtgggatgaa taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga
    23461 acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat
    23521 ctgaagaaca cagaaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac
    23581 cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc
    23641 aacggcggct gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat
    23701 gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa
    23761 tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca
    23821 ggaaggttct ccgttccaga gctctccgtt ctgccagggt ggccagggcg gtaatggtgg
    23881 cggccagcaa cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg
    23941 ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtgattaaag tccagctgag
    24001 cgatggccgt aagttcgacg cgaagatggt cggcaaagat ccgcgctctg atatcgcgct
    24061 gatccaaatc cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact
    24121 gcgcgtgggt gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac
    24181 ttccgggatt gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt
    24241 catccagacc gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa
    24301 cggcgaactg atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat
    24361 cggttttgca atcccgagca acatggtgaa aaacctgacc tcgcagatgg tggaatacgg
    24421 ccaggtgaaa cgcggtgagc tgggtattat ggggactgag ctgaactccg aactggcgaa
    24481 agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc
    24541 cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat
    24601 cagcagcttt gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac
    24661 cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag
    24721 ccagaatcag gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa
    24781 caaaggcaaa gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca
    24841 gatcggcctg aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat
    24901 cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga acattcagcg
    24961 cggcgacagc accatctacc tgttaatgca gtaatctcac tcaacccctt cctgaaaacg
    25021 ggaaggggtt ctccttacaa tctgtgaact tcaccacaac tccatacatc ttcatcatcc
    25081 tttaggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg
    25141 gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta
    25201 ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgtgggcga attatcggca
    25261 gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg
    25321 gacgagtcgt cgatatcgat gatgcggtgg cacgtcatct gcacggtgtg cggcaaggga
    25381 ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac
    25441 cagagaatct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac
    25501 agtcgcggtt gatgcacttg ttggcgcagg atagccgttt gcgggaggaa ctggtgatga
    25561 acctgattca ggcagaggag aatactcccg cacttactga atgggcgcaa cggctgggga
    25621 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggtg
    25681 tggacagcgc aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta
    25741 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
    25801 cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca
    25861 tgaaagagta cggccggctg cgttttcgcg tttcactggg caactatttt accggtcctg
    25921 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga
    25981 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
    26041 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
    26101 acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgtcacaat gtgcaaccgc
    26161 tggcaacgtc aaaggcgttg tttatttatc gtaataccct ggagtatcgg cttaatcgta
    26221 tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg
    26281 cgttacaact ggatgaagag cggtaggtta tgcgttaagg ttgaggcggc gctgtctcat
    26341 tcccctcagc ccgaagggga gaggggaatg cccgaattaa ccgtcaattc gggattattt
    26401 acgcgttagt ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc
    26461 caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg
    26521 gcagatttga tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc
    26581 tttataaccc tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa
    26641 cttaacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc
    26701 gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag
    26761 acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt
    26821 tagcatttgg cctagcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga
    26881 cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg
    26941 ttctacacca gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa
    27001 ctgcccacgc cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg
    27061 taacgtttat cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg
    27121 ccgtgagtat ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg
    27181 tcgggattat gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg
    27241 cattgtccgg cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg
    27301 atcaccgtag cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt
    27361 aagtgatttt tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc
    27421 gcacattcgg caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca
    27481 aataatacgt aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca
    27541 atcgcgtcag aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa
    27601 agaattttga atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca
    27661 aaccagcctg gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg
    27721 gcagtagcct gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg
    27781 gcagcgtcgt gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg
    27841 ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg
    27901 tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgtga
    27961 ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg
    28021 cccatggaaa tgacggaacc ttcttcgaca atcacccctt caactacttc agagcgtgcg
    28081 ccaatgaagc agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg
    28141 ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg
    28201 gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac
    28261 ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca
    28321 acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt cattggcact
    28381 ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag
    28441 agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct
    28501 acacgcagtg caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg
    28561 gtgtctgcgt tggttggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc
    28621 tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt
    28681 ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagcgca
    28741 cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt
    28801 cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt
    28861 cctggctggt cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggtcggcaaa
    28921 aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg acggggctgc
    28981 ggtggctgcc agctactttg cgtcagtact tgctccagac caaaccgaat aacctcatga
    29041 cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc catcgccata
    29101 ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg
    29161 gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct
    29221 ccacgcgtag cctgcgggct aagcaatacc agtggtttgc ttaaatcatg ctgtaataaa
    29281 tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag
    29341 cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt
    29401 gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt
    29461 cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc
    29521 cacagcgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag atagcgcaga
    29581 cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg
    29641 cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt
    29701 gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga
    29761 atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc
    29821 agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt
    29881 tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg
    29941 taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac
    30001 gcgccgagca cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga
    30061 cgcagaatgc tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga
    30121 tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg
    30181 ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt
    30241 tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt
    30301 ttttcgtcgg caggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg
    30361 agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg
    30421 ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg
    30481 cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc
    30541 agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca
    30601 tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc
    30661 aagccgccag ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc
    30721 tgatggcgct ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat
    30781 ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag
    30841 cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct
    30901 tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc
    30961 tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg
    31021 atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg
    31081 tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac
    31141 tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc
    31201 caacgctgga aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc
    31261 cagacgcatg gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt
    31321 tctggaaggg tattcattgt gcgccactca taagattaaa ttatcgcatt aaaaaagccg
    31381 gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt
    31441 gcgtagcgtc agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc
    31501 agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt
    31561 tttacccgcg ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt
    31621 ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc
    31681 gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaact tctgaatcgc
    31741 cgcaccgatt tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct
    31801 ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat
    31861 tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc
    31921 gccatctttc agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga
    31981 gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc
    32041 attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac
    32101 atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc
    32161 gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata
    32221 ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt
    32281 ctgaatttcg ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg
    32341 ctgtacacag cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg
    32401 gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa
    32461 caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac
    32521 gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg
    32581 cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat
    32641 gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt
    32701 accgatgttc aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat
    32761 ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg
    32821 cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt
    32881 tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga
    32941 aaagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag
    33001 cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga
    33061 ccacgaacac attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt
    33121 tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat
    33181 ccgtgctgtg accctgtacc tgggcgctgt agctgcaacc gtacgtgaag gccgttctca
    33241 ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac
    33301 tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac
    33361 atcagtaagt gaccgggatg agcgagcgaa gataacgcat ctgcggcgcg aaatatgaag
    33421 ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc
    33481 ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt
    33541 agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc
    33601 atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa
    33661 aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac
    33721 ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag
    33781 actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca
    33841 gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt
    33901 gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc tgcgctggaa
    33961 ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa
    34021 ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa
    34081 ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg
    34141 gatatagcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc
    34201 atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc
    34261 aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc
    34321 gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg
    34381 tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat
    34441 cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag
    34501 tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac
    34561 ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct
    34621 tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac
    34681 tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg
    34741 cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga
    34801 acggcctggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg
    34861 ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc
    34921 gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact
    34981 cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag
    35041 ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac
    35101 tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc
    35161 tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc
    35221 gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga
    35281 taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt
    35341 attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt
    35401 gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac
    35461 gattacccgt aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg
    35521 tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag
    35581 atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac
    35641 gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtagaatat tacggcacgc
    35701 cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca
    35761 acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg
    35821 gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag
    35881 aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag
    35941 tacgtaacgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga
    36001 tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa
    36061 tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc
    36121 ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt
    36181 ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc
    36241 tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt
    36301 gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc
    36361 cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa
    36421 cagggtagcc gcaccgaagt cttaagtggg caacaagccg cttacgatat ggcagcgctt
    36481 gaggatgttg atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt
    36541 gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc
    36601 ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc
    36661 gaacataacg ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct
    36721 gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt
    36781 gagacgccat tgcgcgattt ggcaacaatg acgccggatc aagcctgccg tcatccgaac
    36841 tggtcgatgg ggcgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa
    36901 tacattgaag cgcgttggct atttaacgcc agcgccagcc agatggaagt gctgattcac
    36961 ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg
    37021 ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac
    37081 tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat
    37141 tatgatcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg
    37201 acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc
    37261 cgctttacgg atatcgctgc gttgaattta tcggtactgg aaaaaatgga tatgcgcgaa
    37321 ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa
    37381 gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtctcg ctatttgtta
    37441 gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg
    37501 tcaggtatgc cgtggttttg cacggctttt tttgtatagg cttcagtatt cctgagtacc
    37561 gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga
    37621 aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg
    37681 ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg
    37741 ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag
    37801 tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg
    37861 ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg
    37921 cgataccagt cgctttaact cgcgtttgca agaatgtatt cgtaaatctg aagcgctaac
    37981 agccgggaat accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat
    38041 agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca
    38101 gatagatgaa gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt
    38161 agtaattagg actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta
    38221 tgccgaactt tactttatag atgttctctg gcccgatttc gatgaacaag actttgaagg
    38281 ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga
    38341 aacagcctga tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat
    38401 acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca ttgtaacgct
    38461 ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc
    38521 gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt
    38581 gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgct
    38641 gggttggtgg attgtctcgc tattgctggt gctgttttac ccaggttccg cagcaatctg
    38701 gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg
    38761 gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg
    38821 gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa
    38881 attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt
    38941 tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt gggcgaatct
    39001 cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct
    39061 cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt
    39121 aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt
    39181 ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt
    39241 tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac tgtgcatgaa
    39301 tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg
    39361 tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatcgccctg
    39421 atcccgctgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa
    39481 ctccgccacc atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca
    39541 ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt
    39601 gtgcctggcg tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca
    39661 caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat
    39721 gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg
    39781 ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag
    39841 cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggct gcaaattgaa
    39901 cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc
    39961 gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt
    40021 gtccgggata acccggggaa atccttagcg ttagaaatcg aaaggcaggg aagtcccttg
    40081 tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt
    40141 attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg
    40201 ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt
    40261 atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct
    40321 atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt
    40381 gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg
    40441 gcgcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt
    40501 caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt
    40561 ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga
    40621 tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag
    40681 ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggccc
    40741 tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc agtaatacca
    40801 ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata
    40861 cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca
    40921 aatcggtgaa agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg
    40981 aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca
    41041 gcgtcggtaa atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg
    41101 ttgacctaaa actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg
    41161 ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag
    41221 tgccgtggtg gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc
    41281 ttgaaaccct gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta
    41341 cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag
    41401 gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg
    41461 aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca
    41521 agatggaaga tgacatcaaa aagcttctcg gtcgctatgg ttatgcttat ccgcgcgtac
    41581 agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg
    41641 cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg
    41701 ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg
    41761 atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc
    41821 aacgtgttcc gggtagcccg gaccaagtag atgtagtcta caaggtgaaa gaacgtaaca
    41881 ccggtagctt caacttcggg attggttacg gtactgaaag tggtgtgagc ttccaggctg
    41941 gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa
    42001 acgattacca gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg
    42061 taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg
    42121 actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata
    42181 actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg
    42241 ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata
    42301 acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc
    42361 gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg
    42421 gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg
    42481 acgatcacaa atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg
    42541 gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc gtccgtggct
    42601 tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg
    42661 atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg
    42721 atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc
    42781 cgtttattag cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta
    42841 ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc
    42901 caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt
    42961 tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc
    43021 agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag
    43081 cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg
    43141 tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt
    43201 ttagcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc
    43261 ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa
    43321 ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg
    43381 cagtccatga aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc
    43441 cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa
    43501 gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag
    43561 gatatcgatc tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac
    43621 atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat
    43681 ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt
    43741 gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt
    43801 gagcatttag gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc
    43861 gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa
    43921 attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg
    43981 acggcgaagc tggggaacaa tgtatcgatt ggcgctaacg cggtgattga atccggcgtt
    44041 gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc
    44101 ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag
    44161 aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat
    44221 cgtggtaact gggttaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag
    44281 atcggtgcct gcactaccat cgatcgcggc gcactggatg acactgttat tggcaatggc
    44341 gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg
    44401 gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc
    44461 ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg
    44521 ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa
    44581 cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag
    44641 cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg
    44701 ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt
    44761 tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag
    44821 agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt
    44881 ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc
    44941 agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac
    45001 aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact
    45061 acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga
    45121 tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc
    45181 tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct
    45241 gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt
    45301 caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcggaattg
    45361 gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg
    45421 ataatgagat ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg
    45481 gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc
    45541 atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga
    45601 tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg
    45661 caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag
    45721 tccatcagtt ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc
    45781 aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata
    45841 tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt
    45901 ataagctgat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac
    45961 tggcggaaac atatccggaa gtgaaagcct ttaccgattt cttttcacgc tcaacgcgcg
    46021 gtctgattcg ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac
    46081 ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg tgcccaacgc
    46141 ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cctggtacga
    46201 aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact
    46261 gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg
    46321 tattgatgcg cctgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa
    46381 aaccattcat tacgtcagtc cgtcagtctg ggcgtggcga cagaaacgtg ttttcaaaat
    46441 aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa
    46501 atacaacgta ccgtgccgct ttatcggtca taccatggct gatgccatgc cattagatcc
    46561 agataaaaat gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt
    46621 gctaccgggg agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc
    46681 ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa
    46741 acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagactttt cagttcattt
    46801 gctggatggg atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg
    46861 tacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa
    46921 gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa
    46981 tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg agccgcaaaa
    47041 actggctgcg gcgctgttac cgctgttggc gaacgggaaa atcagccacg cgatgcacga
    47101 taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc
    47161 cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt
    47221 gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt
    47281 gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt
    47341 ctggcgctct gtgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa
    47401 ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc
    47461 gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa
    47521 ttaccgatgc ctgcgatggt tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc
    47581 gcgtctatcc tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc
    47641 ccgcaatatg gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg
    47701 gctgaacacg gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg
    47761 ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa
    47821 ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa
    47881 actgcaccgt tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat
    47941 ttcaccaacc tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag
    48001 cctatcgtcg gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac
    48061 ctgacggtac tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa
    48121 gcgtatcagc gcgggtacgg tgccgctggg ccgatcatcg atcgcgactg gcttatcgaa
    48181 ttaaatgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg acgcagtctt
    48241 ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg
    48301 gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag ctatctgcac
    48361 gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa cgatgtgcgc
    48421 tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt
    48481 accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa
    48541 gaggagatgt gtgagctgtt tgccgacatc cccgaagccc ttgccaacac cgttgagatc
    48601 gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca gttcccgacc
    48661 ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt
    48721 ctggcctttt tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa
    48781 cgtctggaga ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc
    48841 gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc
    48901 tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa
    48961 tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac
    49021 gttgacttct gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt
    49081 cgtgatgcgg tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc
    49141 gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc
    49201 ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa
    49261 atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg
    49321 gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc
    49381 gattttgcgc cgctttacta cgatgaagag ggcaaacatc cggtcaccca gtttgataaa
    49441 agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc
    49501 atcatcaact gggcgctgg
//
LOCUS       DADLTD010000003        47217 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLTD010000003 DADLTD010000000
VERSION     DADLTD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.003"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            27..764
                     /locus_tag="JN701_000121"
     CDS             27..764
                     /locus_tag="JN701_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7362678.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLSG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            875..1885
                     /gene="pyrD"
                     /locus_tag="JN701_000122"
     CDS             875..1885
                     /gene="pyrD"
                     /locus_tag="JN701_000122"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY7362679.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2059..2601
                     /gene="zapC"
                     /locus_tag="JN701_000123"
     CDS             2059..2601
                     /gene="zapC"
                     /locus_tag="JN701_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY7362680.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="JN701_000124"
     CDS             complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="JN701_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY7362681.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3951..6059
                     /gene="rlmKL"
                     /locus_tag="JN701_000125"
     CDS             3951..6059
                     /gene="rlmKL"
                     /locus_tag="JN701_000125"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY7362682.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6071..7978
                     /locus_tag="JN701_000126"
     CDS             6071..7978
                     /locus_tag="JN701_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7362683.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8108..9361
                     /gene="pqiA"
                     /locus_tag="JN701_000127"
     CDS             8108..9361
                     /gene="pqiA"
                     /locus_tag="JN701_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY7362684.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9366..11006
                     /gene="pqiB"
                     /locus_tag="JN701_000128"
     CDS             9366..11006
                     /gene="pqiB"
                     /locus_tag="JN701_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY7362685.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            11003..11566
                     /gene="pqiC"
                     /locus_tag="JN701_000129"
     CDS             11003..11566
                     /gene="pqiC"
                     /locus_tag="JN701_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY7362686.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11822..11989
                     /gene="rmf"
                     /locus_tag="JN701_000130"
     CDS             11822..11989
                     /gene="rmf"
                     /locus_tag="JN701_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY7362687.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="JN701_000131"
     CDS             complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="JN701_000131"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY7362688.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12646..14406)
                     /locus_tag="JN701_000132"
     CDS             complement(12646..14406)
                     /locus_tag="JN701_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY7362689.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14592..15044
                     /gene="matP"
                     /locus_tag="JN701_000133"
     CDS             14592..15044
                     /gene="matP"
                     /locus_tag="JN701_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY7362690.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="JN701_000134"
     CDS             complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="JN701_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY7362691.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="JN701_000135"
     CDS             complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="JN701_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY7362692.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17245..17874
                     /gene="sxy"
                     /locus_tag="JN701_000136"
     CDS             17245..17874
                     /gene="sxy"
                     /locus_tag="JN701_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY7362693.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="JN701_000137"
     CDS             complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="JN701_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY7362694.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(20009..20455)
                     /locus_tag="JN701_000138"
     CDS             complement(20009..20455)
                     /locus_tag="JN701_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY7362695.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20578..22632
                     /gene="helD"
                     /locus_tag="JN701_000139"
     CDS             20578..22632
                     /gene="helD"
                     /locus_tag="JN701_000139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY7362696.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(22664..23122)
                     /gene="mgsA"
                     /locus_tag="JN701_000140"
     CDS             complement(22664..23122)
                     /gene="mgsA"
                     /locus_tag="JN701_000140"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY7362697.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23218..23880)
                     /locus_tag="JN701_000141"
     CDS             complement(23218..23880)
                     /locus_tag="JN701_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY7362698.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24053..24466
                     /locus_tag="JN701_000142"
     CDS             24053..24466
                     /locus_tag="JN701_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY7362699.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24511..24828)
                     /gene="hspQ"
                     /locus_tag="JN701_000143"
     CDS             complement(24511..24828)
                     /gene="hspQ"
                     /locus_tag="JN701_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY7362700.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24886..26076)
                     /gene="rlmI"
                     /locus_tag="JN701_000144"
     CDS             complement(24886..26076)
                     /gene="rlmI"
                     /locus_tag="JN701_000144"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY7362701.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26171..26449
                     /gene="yccX"
                     /locus_tag="JN701_000145"
     CDS             26171..26449
                     /gene="yccX"
                     /locus_tag="JN701_000145"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY7362702.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26446..26775)
                     /gene="tusE"
                     /locus_tag="JN701_000146"
     CDS             complement(26446..26775)
                     /gene="tusE"
                     /locus_tag="JN701_000146"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY7362703.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26866..27525)
                     /gene="yccA"
                     /locus_tag="JN701_000147"
     CDS             complement(26866..27525)
                     /gene="yccA"
                     /locus_tag="JN701_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY7362704.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27732..27819)
                     /locus_tag="JN701_000148"
     tRNA            complement(27732..27819)
                     /locus_tag="JN701_000148"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27783..27785),aa:Ser,seq:tga)
     gene            28246..29364
                     /gene="hyaA"
                     /locus_tag="JN701_000149"
     CDS             28246..29364
                     /gene="hyaA"
                     /locus_tag="JN701_000149"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY7362705.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29361..31154
                     /gene="hyaB"
                     /locus_tag="JN701_000150"
     CDS             29361..31154
                     /gene="hyaB"
                     /locus_tag="JN701_000150"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY7362706.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            31173..31880
                     /gene="hyaC"
                     /locus_tag="JN701_000151"
     CDS             31173..31880
                     /gene="hyaC"
                     /locus_tag="JN701_000151"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY7362707.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31877..32464
                     /locus_tag="JN701_000152"
     CDS             31877..32464
                     /locus_tag="JN701_000152"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY7362708.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32461..32859
                     /gene="hyaE"
                     /locus_tag="JN701_000153"
     CDS             32461..32859
                     /gene="hyaE"
                     /locus_tag="JN701_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY7362709.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32856..33713
                     /locus_tag="JN701_000154"
     CDS             32856..33713
                     /locus_tag="JN701_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY7362710.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33847..35391
                     /gene="appC"
                     /locus_tag="JN701_000155"
     CDS             33847..35391
                     /gene="appC"
                     /locus_tag="JN701_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY7362711.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35403..36539
                     /gene="appB"
                     /locus_tag="JN701_000156"
     CDS             35403..36539
                     /gene="appB"
                     /locus_tag="JN701_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7362712.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36552..36644
                     /gene="cbdX"
                     /locus_tag="JN701_000157"
     CDS             36552..36644
                     /gene="cbdX"
                     /locus_tag="JN701_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY7362713.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36724..38022
                     /locus_tag="JN701_000158"
     CDS             36724..38022
                     /locus_tag="JN701_000158"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY7362714.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVANGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38137..40317)
                     /gene="etk"
                     /locus_tag="JN701_000159"
     CDS             complement(38137..40317)
                     /gene="etk"
                     /locus_tag="JN701_000159"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HAY7362715.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40337..40783)
                     /gene="etp"
                     /locus_tag="JN701_000160"
                     /gene_synonym="yccY"
     CDS             complement(40337..40783)
                     /gene="etp"
                     /locus_tag="JN701_000160"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY7362716.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40771..41910)
                     /locus_tag="JN701_000161"
     CDS             complement(40771..41910)
                     /locus_tag="JN701_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7362717.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLVEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41956..44052)
                     /locus_tag="JN701_000162"
     CDS             complement(41956..44052)
                     /locus_tag="JN701_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY7362718.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44052..44798)
                     /locus_tag="JN701_000163"
     CDS             complement(44052..44798)
                     /locus_tag="JN701_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7362719.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44795..45439)
                     /gene="gfcB"
                     /locus_tag="JN701_000164"
     CDS             complement(44795..45439)
                     /gene="gfcB"
                     /locus_tag="JN701_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HAY7362720.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45546..45851)
                     /locus_tag="JN701_000165"
     CDS             complement(45546..45851)
                     /locus_tag="JN701_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HAY7362721.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46293..46505)
                     /gene="cspH"
                     /locus_tag="JN701_000166"
     CDS             complement(46293..46505)
                     /gene="cspH"
                     /locus_tag="JN701_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY7362722.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46791..47003
                     /gene="cspG"
                     /locus_tag="JN701_000167"
     CDS             46791..47003
                     /gene="cspG"
                     /locus_tag="JN701_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY7362723.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47014..47202
                     /locus_tag="JN701_000168"
     CDS             47014..47202
                     /locus_tag="JN701_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY7362724.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11626 a        11727 c        12412 g        11452 t
ORIGIN      
        1 tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta
       61 atacgtggaa cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg
      121 aattgcgggc aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat
      181 cgatatctat tttactgact aataccagtc aggaatcctg gctgattaac agtaaaatca
      241 accgcccaac gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg
      301 ctccgccact tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg
      361 aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc
      421 catccggcaa agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt
      481 tctggcgacc cgaaggtttg tcgggcgacc cgctggaagc ttatcaacaa ttacgctgga
      541 cacggaattc gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc
      601 aggtgagcgt aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc
      661 gtcagacaag ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg
      721 attacgacgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac
      781 gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgc
      841 ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc
      901 ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat
      961 tacaggaacg ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg
     1021 catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga
     1081 gtgcattgac gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc
     1141 acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt
     1201 gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa
     1261 ggcccattat gacggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga
     1321 gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat
     1381 cgccatcaat atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc
     1441 gctggatgat ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca
     1501 taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca
     1561 ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact
     1621 cgatcgttct cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg
     1681 tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg
     1741 cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc
     1801 tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat
     1861 taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata agataatcag
     1921 ggctttattt tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg
     1981 tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat
     2041 agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat
     2101 gaagagcacg atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt
     2161 gcgcgcaaga tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc
     2221 gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa
     2281 gccgaactgg tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa
     2341 agctggcatt ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc
     2401 gtttggttga gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat
     2461 gccgcactat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg
     2521 ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc
     2581 agcctcgaac aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag
     2641 caaagaatgg tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct
     2701 aaaatctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt
     2761 ccctgatttt ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc
     2821 tgccagtcaa tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg
     2881 gcagctgcgc cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg
     2941 cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc
     3001 tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct
     3061 ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc
     3121 acatcaccaa tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc
     3181 accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca
     3241 cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca
     3301 gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg
     3361 cgggagaaaa atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa
     3421 tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat
     3481 gcgctactgc catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac
     3541 cgtaccatct ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata
     3601 aagatgcgat cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg
     3661 cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca
     3721 aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa
     3781 ttttcttagc gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct
     3841 cgaaatcctc acgtacttgt gtacgctccg gtttctccgc gctgtccatg tccagactga
     3901 ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc
     3961 tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg
     4021 gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg
     4081 tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt
     4141 gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga
     4201 tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca
     4261 acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa
     4321 atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc
     4381 ataaagaaac cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct
     4441 atcgcgatcg tgctggtatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc
     4501 gatccggctg gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc
     4561 tgattgaagc ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg
     4621 gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc
     4681 aaactcgggc ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg
     4741 acgcacgggt gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga
     4801 tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg
     4861 gcacagtgtt gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg
     4921 cgctgcatag cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt
     4981 tgtttagtgc ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca
     5041 aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc
     5101 cagacagcaa accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca
     5161 aaaaattcga gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg
     5221 atctgccgga atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt
     5281 atgcaccgcc aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg
     5341 ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac
     5401 gccagaaggg caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta
     5461 ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc
     5521 tcgatcaccg tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca
     5581 acctgttctc ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca
     5641 ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga
     5701 atggcctaac cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg
     5761 aggcaaatga acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac
     5821 gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac
     5881 gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg
     5941 atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct
     6001 cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa
     6061 aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc
     6121 gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg
     6181 caacggcgcg ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga
     6241 cggtcgcatt atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg
     6301 taacgttgag ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata
     6361 tctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct
     6421 caacgaactg gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa
     6481 ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct
     6541 ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt
     6601 gctgttgctt gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg
     6661 gttccttaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg
     6721 caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa
     6781 ttacgaccag tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc
     6841 cgagttcgat cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg
     6901 ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga
     6961 acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa
     7021 gatcgttttc gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga
     7081 tttttcggcc caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg
     7141 caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca
     7201 cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga
     7261 taaaacggtg atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc
     7321 acgccacgta ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc
     7381 ggtacgtgcg ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa
     7441 accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga
     7501 actgctggaa gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg
     7561 tcagtttgtc gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg
     7621 tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa
     7681 acagcctgcg gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg
     7741 cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct
     7801 cgaagatctg gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt
     7861 cagtcagcca catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga
     7921 gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc
     7981 gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg
     8041 tttatcaggc atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg
     8101 agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat
     8161 gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac
     8221 aacgttaacc gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc
     8281 actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt
     8341 taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag
     8401 cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat
     8461 tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt
     8521 gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag
     8581 tttcgttaaa ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg
     8641 tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga
     8701 cgacatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg
     8761 tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg
     8821 cccgcgttgt agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc
     8881 gctgcttgta acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac
     8941 ggatttatta ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag
     9001 cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt
     9061 aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag
     9121 tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga
     9181 cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta
     9241 tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat
     9301 gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg
     9361 acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg
     9421 tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca
     9481 gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca
     9541 aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg
     9601 atgatttgac gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc
     9661 ataaagacac cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc
     9721 tgggaacgct gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa
     9781 tggataaata cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc
     9841 gtgtgattct cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc
     9901 gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca
     9961 gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct
    10021 ggaaagatag tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct
    10081 cattgacaac gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag
    10141 ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg
    10201 attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc
    10261 tgcaaccggg agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc
    10321 cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga
    10381 ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc
    10441 accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca
    10501 ctggtgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc
    10561 gtgaatttaa tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc
    10621 aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac
    10681 aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg
    10741 atagcatgaa caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt
    10801 caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca
    10861 acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc
    10921 tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc
    10981 cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct
    11041 ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag
    11101 cggtacacaa agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt
    11161 tcctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat
    11221 tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc
    11281 caacctgagt acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca
    11341 ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt
    11401 cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga
    11461 aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag
    11521 tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt
    11581 aagtataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa
    11641 aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga
    11701 cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga
    11761 ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg
    11821 catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg
    11881 catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg
    11941 gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt
    12001 taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc
    12061 agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac
    12121 gaccatcaac cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc
    12181 gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt
    12241 tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga
    12301 accctactag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa
    12361 tgaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc
    12421 ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt
    12481 tcggtgctgg caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa
    12541 ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat
    12601 tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa
    12661 ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg
    12721 cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag
    12781 atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc
    12841 ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg
    12901 gataatgaca ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc
    12961 gattttctca tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt
    13021 gatagcgata ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc
    13081 agccatcgag gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc
    13141 tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat
    13201 catccctttc gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc
    13261 accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg
    13321 acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc
    13381 aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg
    13441 ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca
    13501 cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc
    13561 ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg
    13621 ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga
    13681 ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag
    13741 ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt
    13801 cagcttcaat ggcatcgaag gcacagagac ggggagaggg cgcgactcat caaacgcaac
    13861 ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc
    13921 cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg
    13981 ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac
    14041 ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg
    14101 acgtaagcgg atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc
    14161 gctttgtagc gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc
    14221 cttagccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg
    14281 cagccgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat gtggctgagc
    14341 aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat
    14401 ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact
    14461 acagcacacg tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg
    14521 gagattcttt tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca
    14581 ttgagatcgc tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt
    14641 atctggtgaa gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg
    14701 cccaggaagc cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct
    14761 ggattgacaa gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca
    14821 gacggaagcg ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg
    14881 aatttattgt ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg
    14941 aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt
    15001 cttctctgaa gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt
    15061 caatttacta aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact
    15121 taagcctgcg gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga
    15181 tccggagcca ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca
    15241 gtaaccgggt tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg
    15301 gagatcaggt aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg
    15361 tcagaaccga tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg
    15421 ttgctcagct ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct
    15481 ttgttgaagt tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt
    15541 gccggagctg gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa
    15601 acacccaagc tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg
    15661 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    15721 ccgccagcga agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta
    15781 gtgtctgcac gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt
    15841 gggtaaccca gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg
    15901 ctgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg
    15961 ttaacctggt aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg
    16021 ttgttgatga aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag
    16081 gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc
    16141 gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat
    16201 ctccaacgag ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac
    16261 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    16321 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta
    16381 atcttttttg tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct
    16441 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    16501 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    16561 tcgtggcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    16621 ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga
    16681 tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag
    16741 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    16801 cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt
    16861 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    16921 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    16981 cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc
    17041 ctgtgagtta ctgtatgaat gtacagtaca tccagtgaca acaaagatca accctatttt
    17101 cggaaagagc ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc
    17161 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    17221 tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    17281 caagaatacc tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc
    17341 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    17401 tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag
    17461 tgtggccgat ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa
    17521 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    17581 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    17641 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    17701 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    17761 ggtgccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    17821 gaatgggctg actcacttac gccgaaacag gagtttcctg cggaacttga gtaatctggc
    17881 gttgcagacg accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca
    17941 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta
    18001 atgcctcatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
    18061 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    18121 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    18181 cgcgaatttg cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct
    18241 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    18301 cctgatggta ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt
    18361 cgagcatgcg cggcaaattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc
    18421 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt
    18481 cacccagtaa gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt
    18541 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc
    18601 cttccagtga tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac
    18661 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    18721 aacttgtcag caagatccaa aacccgtgat gcattccggt tatttgaatg atggcgtagc
    18781 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    18841 agtgacggct aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct
    18901 cattttcgtc attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt
    18961 gggcatcaat ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg
    19021 gtgcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    19081 aatgcggatc atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg
    19141 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    19201 gttcacgcaa tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg
    19261 cgacaaagta ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc
    19321 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag
    19381 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    19441 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    19501 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    19561 gcagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    19621 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    19681 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    19741 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    19801 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta
    19861 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    19921 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    19981 gacttagcat aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac
    20041 gcgcagcttg cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag
    20101 cggcaatttt aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga
    20161 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    20221 tcaataccgt accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga
    20281 catgaatagc ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc
    20341 gtgtcagcgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca
    20401 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    20461 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    20521 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    20581 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    20641 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    20701 caattactgg cgattcactg taagcgtggt ctggtatggg gcgagctgga atttgtactg
    20761 ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac
    20821 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt
    20881 gttttacgcc agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg
    20941 cgtgagcaaa cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg
    21001 ccggttaacc gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc
    21061 tggttgaaag atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg
    21121 cttaccgagt atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag
    21181 gcccgggcag tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga
    21241 aaaacgtcgg tgctggtggc ccgtgcaggc tggttgctgg cacgtggtga agcgtcccct
    21301 gagcaaattt tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcggatt
    21361 cgcgaacggc tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat
    21421 attattcagc agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct
    21481 gcccgtcatg aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag
    21541 gcgaagggct ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac
    21601 ttctgggatg atgaaaaatt acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt
    21661 ctgatgcgga tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt
    21721 cgcgatctgt tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt
    21781 gcgctgaagg cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt
    21841 ctggagaaag gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat
    21901 atctcgccgc agcgggcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg
    21961 ttgttcgctg ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg
    22021 ctcaccaccg ctttccatga aaactttggt gaaggcgaac gttgtgattt agacacgact
    22081 taccgtttta acagtcgtat cggtaaggtg gcaaaccgtt ttattcagca gaacccaggc
    22141 cagctgaaaa agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg
    22201 gatgagagtc aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag
    22261 cgcattctga tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca
    22321 acacgctggc cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag
    22381 gcggattacg tcatcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg
    22441 cgggagtcga ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa
    22501 gaacggcggt taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt
    22561 aacaaagaga atccctctcc ctttgttgaa atactgaaaa atctggatgt gccggtggcg
    22621 agaaaaccgt aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg
    22681 cgagataacg ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact
    22741 ggattatgaa gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca
    22801 gacgcagcaa ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga
    22861 aaatcaatac atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca
    22921 ttgggccact caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg
    22981 tagtgcctgt tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc
    23041 tcatcagcat ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta
    23101 aagtgcgagt cgtcagttcc ataatgtaca tccgtagtca actttcctac agattactgt
    23161 aagcacttat cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca
    23221 ggaagatggt tgtttttccg cccactgcag gaaagtattt cgcgtttgtg ggtcagccag
    23281 tttaaaccaa tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc
    23341 cagctcggaa acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg
    23401 tagtgaagcg cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt
    23461 ttttgccgtt gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc
    23521 atccaacaat tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag
    23581 gcgaggcagg cgaaaattga cctggttgat gagctgggta ttaaaactga ccactagcgg
    23641 tggggagata tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa
    23701 cactaactga tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct
    23761 ggaaaccttc ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt
    23821 ggcaaaaacg gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat
    23881 aggtagctcc tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat
    23941 acttactaac atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat
    24001 ggttggcatg tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga
    24061 aaccgatatt gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga
    24121 caaacccgat cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcatgt
    24181 cattccggtt tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtatggcac
    24241 gctggcagac gttccggaaa aggtcgatat ggtcgatgtt ttccgtaatt cagaagcggc
    24301 gtggggcgtg gcacaggaag ctattgccat tggcgcgaaa accctctgga tgcaactggg
    24361 ggtgattaat gagcaagcgg cagtactggc acgggatgcc gggttgaatg tggtgatgga
    24421 tcgctgcccg gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag
    24481 gcaaaaacct tccggggatt cgttcaggga tcagttacgc agacgcgggg cctggagttg
    24541 tttgcggatg gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa
    24601 ttcgctactt agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc
    24661 catcactacg tgataccacg gagcggcgcg aagctcgtca tttaccgcca gctcatcagg
    24721 cgacggttca gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa
    24781 cagggaatgg cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg
    24841 ggaaatcttt acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca
    24901 ggcaaaccct ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggctgc
    24961 ctgacggaac tgctctataa attgtacatc acggccggca tcaattgcgg catccgcgat
    25021 gattttctga aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc
    25081 gccttcattc agcaactgaa tcgccagcat gttgatatct ttatagccac gacacgcgcc
    25141 catcaactgg cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt
    25201 ttcaccgcga tcgcgataag tacgcagcaa tttaaagaca tcatcacgga caaactcagc
    25261 cttgctcaga tccagtttgt tcagctcaac gttctgccgt gcaatatcca gcgcttcctg
    25321 ggaggtatca acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc
    25381 accggtatag gagaaacagt tcagcacacg tttattttca acgtagcggc gggtagccag
    25441 gcggctgtct cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag
    25501 cagcttcatg ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgacgag
    25561 gcccagggtc agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat
    25621 cgcacattcc gggtacagcg tttgcagggc actaactaat gccgcgcgct gatattctgc
    25681 cccagcactc agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg
    25741 caggccatca gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag
    25801 ccagtcacgc catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga
    25861 ctcagacggg tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc
    25921 gcgtgctaac cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt
    25981 accttccatg cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt
    26041 ttcgcgccct ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa
    26101 gaaatgggcg tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag
    26161 gagagcagca atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt
    26221 aggatttcgc tacaccacac agtacgaagc gaaaagactg ggtttaaccg ggtacgccaa
    26281 aaatcttgac gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtggaaaa
    26341 attaatgcag tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag
    26401 cgagccgcat catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac
    26461 cggttttggc aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg
    26521 gtataaatag cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag
    26581 catacgaatc gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac
    26641 ttcccagtgt tccggtgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg
    26701 ctcactccac tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc
    26761 ttcgaagatc agcataatta ttactacctt aatcagactg gcggcagtgt aacaaaaaac
    26821 aaagccccgc ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc
    26881 gaagcccaga atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac
    26941 cgtggcacga atatagttcg tttcaccgcc atgaacgatg ttgctggttt caaacaaaat
    27001 agcgccagag gagatcaaaa tgaagaccgc gctgatcgcc agatgcagag caggcagctg
    27061 caggaagata ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc
    27121 gccgaggaac gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac
    27181 taacgccgtt ccgcccagag ccatagcgat tacgtcaccc attccggcag acagataggt
    27241 gttcagaata ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat
    27301 cccggtcggc ttattcgccg ttttataggt caggaacatc aaaccataca tacctaccag
    27361 cgtcagaatc agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc
    27421 cgaaaaggcc agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct
    27481 aagcagtgat gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat
    27541 gacagatgta attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac
    27601 ccatctttac gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa
    27661 atataacgcc ctgagaattt cgacaggcaa aagaaaaagg ggttagcatt tagctaaccc
    27721 cttatcttat ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt
    27781 tttcaagacc ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac
    27841 atcccgatgc ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg
    27901 cattgatagc gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt
    27961 tactcgttta gcaaccggct aaacatccct accgcccggc aaaaagaaaa ataggtccat
    28021 ttttatcgct aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac
    28081 gctttattaa caatactttc tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt
    28141 cagcatctca cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccataa
    28201 gcgcccggtg tcctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa
    28261 acattttacc aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt
    28321 agtctggctg ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg
    28381 ctggagaaca aaccccgcat tccggtggta tggatccacg gtctggaatg cacctgctgt
    28441 accgaatctt ttatccgctc cgctcaccca ctggcgaagg acgtcatcct ttccctgatt
    28501 tccctcgatt atgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttc
    28561 gaagacatca tcacgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg
    28621 ctgggcgagc aggggatgtt ctgtatcagc agcggtcgac cgtttattga gaaactcaaa
    28681 cgtgccgctg ccggagccag cgcgattatc gcctggggaa cctgcgcgtc ctggggctgc
    28741 gtgcaggccg cgcggcccaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac
    28801 aaacccatta tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt
    28861 acttacatgg tgacctttga tcgcttgcca gatgtcgaca gaatgggccg tccgctgatg
    28921 ttctatggtc agcgaatcca cgataaatgc tatcgccgcg cccacttcga cgccggagag
    28981 ttcgtccaga gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc
    29041 tgcaaagggc ctaccaccta taacgcctgt tcctccacac gctggaatga tggcgtttct
    29101 ttcccaatcc agtctggtca cggctgcctg ggctgtgcgg aaaatggttt ctgggatcgc
    29161 ggttcgttct acagccgcgt ggtcgatatt ccgcaaatgg gtactcattc caccgccgat
    29221 accgtcggtt taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc
    29281 agcgccgttg accagcgcag acgtcataac cagcaaccta cagaaaccga acatcagcca
    29341 ggcaatgagg ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaata
    29401 atgccggacg ccgcctggtg gtcgacccga ttacgcgcat cgaaggccac atgcgctgcg
    29461 aagtgaatat taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc
    29521 gcgggctgga gatcatccta caagggcgcg acccgcgcga tgcgtgggcg ttcgttgaac
    29581 gtatctgcgg cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg
    29641 ctatcggtat taaagtgccg gacaacgcca atatcatccg caacattatg ctggcaacgc
    29701 tctggtgcca cgatcatctg gtgcacttct atcagcttgc cgggatggac tggatcgatg
    29761 tgttagatgc gctgaaagcc gacccgcgga aaacctccga actggcgcaa agtctctcct
    29821 cttggccgaa atcatcccct ggctatttct tcgacgtaca aaaccgcctg aaaaaatttg
    29881 ttgaaggcgg gcagttgggg atcttccgca atggctactg ggggcacccg cagtacaaac
    29941 tgccgccaga agctaacctg atgggctttg cccactatct cgaagctctc gatttccagc
    30001 gtgaaattgt caaaatccac gcggtctttg gcggtaaaaa cccgcatcca aactggattg
    30061 tcggcgggat gccttgcgcc atcaacattg acgaaagcgg cgcggtcggg gcagtcaata
    30121 tggaacgcct gaacctggtg cagtcaatta tcacccgcac ggcggacttc attaacaacg
    30181 tgatgatccc cgacgcctta gccatcggtc agttcaacaa accgtggagc gaaatcggca
    30241 ctggtctttc tgataaatgc gttctcagct acggcgcatt cccggatatt gccaacgact
    30301 ttggcgagaa aagtctgctg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg
    30361 tgctgccagt ggatttggtt gatccgcagc aggtgcagga gtttgtcgac cacgcctggt
    30421 atcgatatcc caacgatcag gtcgggcgtc atccgttcga tggcatcacc gacccgtggt
    30481 acaaccccgg cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac
    30541 gctactcgtg gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg gggccgctgg
    30601 cgcgcacgtt aatcgcttat cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgca
    30661 tgatgtcggc gttgaacctg ccgctttccg gtatccagtc aacgttaggc cgcattttgt
    30721 gccgcgcgca cgaagcgcag tgggccgcag gtaagttgca gtatttcttc gacaagctga
    30781 tgaccaacct gaaaaacggc aatctcgcca ctgcttccac ggaaaaatgg gaacctgcaa
    30841 cctggccgac agagtgccgt ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc
    30901 actgggccgc cattcgcgat ggcaagattg atctctacca gtgcgtggtg ccgaccacct
    30961 ggaacgccag cccgcgcgat cccaaagggc agattggcgc ttatgaagcg gcgctgatga
    31021 acaccaaaat ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg
    31081 acccgtgcct cgcctgttca acacacgtgc tgggcgacga cggtagcgag ctgatctccg
    31141 tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca
    31201 gccactatgt ctttgaagcg ccagtccgca tctggcactg gttgacggtg ttatgcatgg
    31261 cggtgttgat ggtcaccgga tactttatcg gcaagccgct accttccgtc agcggcgagg
    31321 cgacgtatct gttctatatg ggctacatca ggttaattca cttcagcgcc gggatggttt
    31381 ttaccgtggt tttgctgatg cggatctact gggcttttgt tggcaatcga tactcccgcg
    31441 agctgtttat cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc
    31501 gctggtatct gtttctggca aaacgtccga gtgccgatat aggccataat cccatcgccc
    31561 aggcggcgat gttcggctat ttcctgatgt cggtctttat gatcatcact ggttttgcgc
    31621 tgtacagcga acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt
    31681 tctactggac gggtggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc
    31741 tgattggcgc gtttgtgatc ggtcatgtct acatggcgct gcgtgaagac atcatgtccg
    31801 acggcacggt gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa
    31861 gtaacaagga gtgttcatga gcgagcaacg cgtggtggtc atggggctgg gcaacctgct
    31921 gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg
    31981 gcccgagtat gtggagattg tcgatggcgg tactcaggga ctgaacttgc tggggtatgt
    32041 cgaaagcgcc agccatctgt tgattctcga tgccattgac tacgggctgg aacctggaac
    32101 gctgcgaacc tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct
    32161 gcatcagaac agtttctccg aagtgctggc gctggcagat atccgcggac atctgccagc
    32221 acatattgcc ctcgtcgggc tgcaacccgc gatgctcgac gactacggcg gcagcctgag
    32281 cgaactggca cgggagcaac tgctcgctgc ggaacaatcg gcgctggcgc agttggctgc
    32341 gtggggaagt gtgccgcaac tggctaatga atcgcgttgt ctcaattatg actgtctgtc
    32401 gatggaaaat tacgaaggcg ttcgcttgcg ccagtaccgg atgacacagg aggagcaggg
    32461 atgagcaacg acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg
    32521 ccggtcagtg aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta
    32581 ttaagcagtg acccgaaacg cacgccagag gtcagcgata atccggtaat gattggcgaa
    32641 ttactgcacg agtttcccga ctatacatgg caggtggcga ttgctgacct tgagcagagc
    32701 gaagccatcg gcgatcgctt tggcgtcttt cgctttccta ccactttagt gtttaccggc
    32761 ggaaactatc gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg
    32821 cgcgggcttg tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt ttccatctgc
    32881 tggggccagg aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct
    32941 gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg
    33001 tgattgcgct gttaaacgag ttacaacatc aactaagcga acgccagccg ccgttgggcg
    33061 aggtgctggc agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc
    33121 ttctcgggga aggggaagtg tcagtgcgca ttcagcaggc tgacgacagt aaaagtgaaa
    33181 tacaggaggc gatcttctgc ggattatggc gggtgcgcag acgtcgcggc gaaaagttgc
    33241 tggaggacaa actggaggct ggctgcgcgc cgctggcgtt gtggcaggcg gcaacgcaaa
    33301 atctcttgcc gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac
    33361 cgttggcgca tgagttactg gcacatgtac gtaaccccga cgcgcagccg cacagcatta
    33421 atctgacgca attacccatc agcgaggctg atcggctttt tctctcacgt ctctgtgggc
    33481 cgggaaatat tcagattcgt accattggct atggcgagag ctatatcaac gccacggggt
    33541 tacgccatgt ctggcattta cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt
    33601 atgaaatctg cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg
    33661 cgcagcggct tagcgaggta tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg
    33721 gtaagtatcg ctttcagtct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt
    33781 ttcaggataa agagggagat ctaacattat cgggttattt ttctctcttc gcctacagga
    33841 gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat
    33901 cactttttat ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc
    33961 atttacgtgg tcaccggcaa aacaatctac cgcgatatga cgcgcttctg gggtaagctc
    34021 ttcggtatca attttgctct tggcgtggct accggcctga ccatggagtt tcagtttggt
    34081 actaactggg cattctattc caactatgtg ggcgatattt ttggcgcacc gctggcgatg
    34141 gaagcattaa tggccttctt cctcgaatcc acctttgtcg ggcttttctt cttcggctgg
    34201 cagcggctga ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat
    34261 ctctctgcat tgtggatatt gaatgccaac ggttggatgc aatacccgac cggtgcgcat
    34321 tttgatatcg acaccctacg tatggagatg accagcttca gcgagctggt ctttaacccg
    34381 gtcagccagg tgaaatttgt gcacaccgta atggcgggct acgtgaccgg ggccatgttt
    34441 attatggcga tcagcgcctg gtatttactg cgcggacggg agcgcgatgt tgcattacgc
    34501 tcgtttgcca tcggttccgt cttcggtact ctggcgatta tcggcacgct acaactcggg
    34561 gacagttctg cgtatgaagt cgcgcaagta caaccggtaa aactggcggc gatggaaggg
    34621 gagtggcaaa cggaacctgc tcctgcaccg ttccatgtgg ttgcctggcc ggaacaggat
    34681 caagagcgta acgcctttgc catcaaaatt cccgcgctgt tagggatcct cgccactcac
    34741 tcattagata aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta cccacgcttg
    34801 caacgcggac gtatggcctg gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg
    34861 catgtgttgc aggcattccg ggaactggaa ggtgacctgg gctacggcat gttgctctcc
    34921 cgctatgcgc cggatatgaa tcatgtcaca gccgcacagt accaggcggc gatgcgtggc
    34981 gcgatacctc aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttcc
    35041 ctgctgctac tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag
    35101 catcgctggg tgctgaaaat ggcgctctgg agtttgccgt tgccgtggat tgcgattgaa
    35161 gccgggtggt ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg
    35221 acatactccg cgcactccgc tttaaccaca ggacaactgg ctttctcact gatcatgatc
    35281 gtagggcttt acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt
    35341 ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aaggagaaaa
    35401 tcatgtttga ttatgaaaca ttgcgcttca tctggtggct gctgattggc gtgatcctgg
    35461 tggtctttat gatctccgac ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg
    35521 cgcgtaatga tgatgaacgc cggatagtga taaacagcgt tggtgcacac tgggaaggca
    35581 accaggtctg gttgatcctc gctggtgggg cattatttgc cgcctggccc agagtgtatg
    35641 cagcggcgtt ttccggcttt tatgtggcga tgatcctggt gctgtgctca ctgttcttcc
    35701 gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg
    35761 acgccggtct ggtcatcggc agtctggtgc cgccggtagt cttcggtatc gccttcggca
    35821 acttgttgct cggcgtgccg tttgccttca caccgcaatt acgcgtggag tatctcggca
    35881 gcttctggca actgctgacg ccattccctt tattgtgcgg attgctcagc cttgggatgg
    35941 taattttgca aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt catctgagtt
    36001 cacagctggc caccaaacgc gctgcactgt tggtgatgct gtgctttttg ctggcgggtt
    36061 actggctgtg ggtcggtatt gatggctttg tactgctcgc ccaggatgct aacggtcctt
    36121 ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg
    36181 aatcgcccgt tttgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga
    36241 tggcgattta tcgtggtcgc ccaggttggg gatttttaat ggcgtcattg atgcaatttg
    36301 gcgtgatttt cacggcaggc atcacgctgt tccccttcgt catgccgtca agcgtgagtc
    36361 cgatctccag cctgacgttg tgggacagca cttccagcca gctgacgctg agcattatgt
    36421 tggtaatcgt gctgatattt ttgcccattg tgttgctcta cactctctgg agctactaca
    36481 aaatgtgggg gcgcatgaca acagaaactc tccgccgtaa cgaaaacgag ttgtactaag
    36541 gagcaggaac aatgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct
    36601 ccacccttgt gttggtatgg ctggacccgc gtctgaaaag ttaacgaacg taggcctgat
    36661 gcggcgcatt agcatcgcat caggcaatca ataatgtcag atatgaaaag cggaaacata
    36721 tcgatgaaag cgatcttaat cccattttta tctcttctta ttccgttaac ctcgcaatct
    36781 gcattcgctc agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat
    36841 ggtgtgcgtg ctccaaccaa ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg
    36901 ccaacctggc cggtaaaact gggttggctg acaccgcgcg gtggtgagct aatcgcctat
    36961 ttcggacatt accaacgcca gcgtctggtg gccaacggat tgctggcaaa aaagggctgt
    37021 ccgcagcctg gtcaggtcgc gattattgct gatgtcgacg agcgtacccg taaaacaggc
    37081 gaagccttcg ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat
    37141 acgtccagtc ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca actggataat
    37201 gcgaacgtga ctgacgcgat cctcagcagg gcaggagggt caattgctga ctttaccggg
    37261 catcggcaaa cggcgtttcg cgaactggaa cgggtgctta attttccgca atcaaacttg
    37321 tgccttaaac gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt accatcggaa
    37381 ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg
    37441 acggagatat ttctcctgca acaagcacag ggaatgccag agccggggtg gggaaggatc
    37501 accgattcac accagtggaa caccttgcta agtttgcata acgcgcaatt ttatttgcta
    37561 caacgcacgc cagaggttgc ccgcagccgc gccaccccgt tattggattt gatcatggca
    37621 gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg tgacattacc cacttcagta
    37681 ctgtttattg ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac
    37741 tggacgcttc ccggtcagcc ggataacacg ccgccaggtg gtgaactggt gtttgaacgc
    37801 tggcgtcggc taagcgataa cagccagtgg attcaggttt cgctggtctt ccagacttta
    37861 cagcagatgc gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg
    37921 accctggcag gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc aggttttacg
    37981 caaatcgtga atgaagcacg cataccggcg tgcagtttgt aatgcataaa aaagagcatt
    38041 cagttacctg aatgctctga ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg
    38101 aaaaggcgtt cacgccgcat ccggccactt tcagttttac tctttctcgg agtaactata
    38161 accgtaatag ttatagccgt aactgtaagc ggtgctggcg cgtttaatca caccattgag
    38221 gatagcgcct ttaatattga cgcctgcctg ttccagacgc tgcattgaca aactcacctc
    38281 tttggcggtg ttcaagccaa aacgcgcaac cagcaggctg gtgccaacag aacgccccac
    38341 gaccgcggca tcactcaccg ccagcatcgg cggcgtatcg acaatcacca gatcgtaatg
    38401 gtcgttcgcc cattccagta attgacgcat ccgatcgcgc atcagcagtt cagacgggtt
    38461 aggtggcacc tgaccgcgag taatcacatc aaagcctcct ttgccaaaat gctggatcac
    38521 tttgttgagc tcatctttac ctgccagata ttccgacaag ccatgttcat tactcacggt
    38581 aaacaggtta tgcgaataac cacggcgtaa gtcggcatca ataaataaca ctttttgatc
    38641 ggactgggcg atcaccgctg ccagagttga actgacaaac gttttaccac tgtctggcgt
    38701 cgcaccggtg atcatcagaa tgttattctc cgtctccatc atagcgaagt gcagactggt
    38761 tcgtagcgca cgtacggctt ccacagcaga atccgccggg ttatccaccg ccaggaaggg
    38821 gatattttta gtacgatggc gctgctgatt agaaaataaa tttttcttac gcagacgggt
    38881 acgtttatcc agccactcgg acattgggat agtggcataa acgctgatgc cgtgctcttc
    38941 cagttgttcc ggggcttcta caccacgacg caacatcgca cgcgccagca cggcacccac
    39001 cgaaataaac aggccaagaa taaaaccaag caccacgttc aacgcttttt tcggtttcac
    39061 tggctgcggc tgagtgactg ctgggtcgat aatccgcacg ttaccaatgg cactggattt
    39121 cgaaatactc aactcctgct ggcggttaag taattgcaga tataccgcac ggcccgcttc
    39181 tacgtcacgg cttaaacgta acacttcctg ttgagtggaa ggcattgccg atacccgctt
    39241 attcaggcgt ttgcgttctt gctccagcgt ctggcgtttt tccagcagcg cacgataagt
    39301 tgggtgatct ttcttataca gctgggagat ctctgcctcg cggaaagtca gctcattgag
    39361 ttgattatca acgttcacaa tctgctcaag aacggctttg gcttctaggt taaggtcaac
    39421 cgaatcgcgc tgctggcgat aaacgttgag tttttcttcc gcctggtcca gctcgctgcg
    39481 cacttcaggc aactggcgct gtaagaattc aaggctttgt gaatcctgcg ccgcctggcg
    39541 agcgatattc tgttgcaaat agttgttagc gatgctgttc agaatacgag taatcaactg
    39601 gggatcatca ccagtcatgg taagttccag catcccgctt tctttactgc gttcgctaac
    39661 ggtaaaggtt tcctgcaatg cgttaatcgc ttccagttcg gtacgctggc tcaggacaaa
    39721 ctgtgttcct ggtttggcct taatgtccgc gatagtcagc gcaacgccat ctttttccag
    39781 acgctggccg accataccat tgacggtgaa ctcttcacct tccagtgtat agtggccgtt
    39841 ttccccaacc gtgagtgtca gttgctgatc ctgaccattc agttgtggaa tatgcatcca
    39901 gctgatcgcc agctcacctg gtttttcttt ggttaatctc gcccagccgc gacccacaat
    39961 cggaaaatac ttctgctcaa ctatgtcgcg cagattcagt tcagcaatgg ttttaccgag
    40021 aatcatgcgc gattgcagca gttggatctc cggtgcagac tcgggcgatg agttagggat
    40081 catatcgctc aggccgctga gaatggcgtt gccctgtttt tgctcaacct ggaccagagt
    40141 atctgcctga taaattggtg tgcttaacag cgagtaagcg acagcgatca gcgtgaataa
    40201 cgcggttacg ctgataataa acttacggtg atcccataac tcgccgacca gacgaagcag
    40261 atcgatctca ttttcctgag tgctgcctgg tggcgtattc atatttttag ttgtcatcgt
    40321 attttataaa ctcaaattac cggctgaggc gcttcgccca ttcctgactg gcgcgctcca
    40381 acataccgta gacatgttca aatgcgtcct gacttttacg atagggatcc gggatctctt
    40441 tctgttccag ccattgccca aacagcattg tcttcccgcg gacctcggga gcgattgcgg
    40501 taacctgagc aatatgctct gactccattg ccagaatcag atcgtaattt cgtgccatct
    40561 cagcagtgag cttacgtccg gcatgtcctt caagagatac gccatgatta gcggcaacat
    40621 ctgctgctgt cgcatctgca gggtgtttta ccagaccatg aacacctgcc gatttgactt
    40681 ttacgcctgg cagacgttta cgcagcaagc gctcgccaat gggcgaacgg caaatgttgc
    40741 cggtacaaac caccaggatt gagttaaatt ttagttgggc catgtttgaa tccaccgtga
    40801 agtctctgtc aggtcatgca ccccggaaat cgtcggtacc agcagagaaa tcacacggtt
    40861 ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg taaggttcaa gctggaattc
    40921 cgttccgagt atcattgccg atgcgtcctt cgcattcagc tgataaatat tggcgatttt
    40981 gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat cagcgacatc
    41041 ctgattcata ccctcagcgt tacccagcgc ttccaccagg gtcataccgc tgcggtccat
    41101 tttcagcgtg ctctgtttac ccacttcacc catcacgaag actttcagat catcgttgcg
    41161 cggaataaat aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt cgccacgctg
    41221 catcagcgca tagaggttca ctttggtctt tacgccattc tgggtgagca ccacgttgcg
    41281 ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc gcatccatga tggtcagagg
    41341 aatattggta atcggctgct gacccgattt gctgacttcg ccggtgacgt acgctttttg
    41401 cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg aatcgagacg
    41461 cgctgtaatc tcgttgcgta cctgggtaag tgttttcccg gccactttca ggcgaccaat
    41521 ataggggtag aaaatcgcac catcggcgtt aacccagttg ccggtatcac tggcgctacg
    41581 gtattgccct gcgggggtgg ttagttccgg gtgatcccag accgtaacca tcaacacatc
    41641 accaatcccg atacgatatt catagtttgc gatttgctgc tccagctccg ggttagcctg
    41701 cgacataatc ggtttggcgc gcaactgatc aatcaatcct ggcgtgacgg gatagatatt
    41761 gaccattttg tccaggtcat actgattgtc aggtagttca atgacatcct tgttcctggt
    41821 cgataaattc tgaccaggaa caagggtaca tgctgtcaaa gacagaacag ccaatgtcag
    41881 taccgaaaac ttaaaaatgt tctttttcat tggtttatat ttatcagtca atgtaatgtt
    41941 atgaattacg tcaggttatt gtccaaccgg tacttctcgc tctgccgtca ttggatagag
    42001 ctggtatttg cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc acgaaaccac
    42061 cgcgcggttg cggttaggtc caatggtcat caagtcgaat ggaatcgaga tataaaaacc
    42121 tttactaaag ccgccttcgc cgtagtcatc tttagatacg ttactgattg ccgcccatac
    42181 ccctaccgcc acgccgctgt caaagcgttt ggcgacgtcg atcgttgctc ctttatcttt
    42241 tgccagatat tgcccaacgc taagtttcat cagtacgcca ttgagcgtcg gcgggttcca
    42301 gtaagccgtc acgaaaccag ttggcgtgga ataatcggtg aagcgcatca tgttgtccca
    42361 gtcacgttgc ttcacgtagt taacgtccac acccagcgcc cagctggcat ctagcgggcg
    42421 ataaagcagc tcggaaccga caccggcgta catcgtttcc agataaccgc catacacctg
    42481 gccatagaaa ccattgccta agtcggcaaa gtagttcgcc tgcaagttgt tgagataaac
    42541 gtcattgcga acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag agtccgcggg
    42601 caacagcgaa gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa taccgccatc
    42661 aagcagcagg tggtcggtaa accagtagcg ggcactggac atcagcccca gctggaacat
    42721 atagaaatct tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag agtagctaaa
    42781 gcgatcttca cgaatacggt agccccgacc aaaggcagaa agatcttctg cttcaacacg
    42841 ttgttgttga agttgctctg attgaccagg cgctgtacca gccagctgtt tgcgcaggct
    42901 ggctacgtcg gtttcggtag tcaccatcgc catatgctcg cgcttttgcg tcacgctaat
    42961 cttctcaacg ccttgcggca ggttattcac cagaatccgg ttggcacgat cgaccgcttc
    43021 gcgagaatca cggtatttgt attgttgacc agacatatac agcgtcttat cgcgcagctg
    43081 aatttccggt gcttcaaaac ctgcgttgta cttcagcgcg gtaagttggt tcgctactgt
    43141 ggtgtactgc aatccttcag attcaggcgc aggttgatat gccggttttg gcgtatcgcg
    43201 cagggcaggg cgcagatcgt tgaagttggt ccgtaacgtg aagccaaaca tcaacgtgtt
    43261 accgcgttca taactcaggt tgagatctgc ccagctggca gcgcgataaa ctgcgccgac
    43321 gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca ttctggtaat tgttgccatc
    43381 gtattcgagt ttcagacgca ggggattcca cggcgtttga tactcaatgc cgccaaagat
    43441 ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt gagactctgc
    43501 gcgatgacaa tatttatcgt tcacccggca aaacgggttg gtaatattgc ccgcattacc
    43561 ggcgtagccc catgccatcc cgagggtgaa atcaaatggc cccgcttgct tgctggccac
    43621 cagatactca ccgtcaaaca gacccgtacc agcaatatca cgtttaccaa acgccacttg
    43681 cggtagccag taaccttctt cccacaggcg aagtttaaaa tcgaatgatt tgtctttgta
    43741 tgactgatcg ccgctgaaat cttcccactg gctatatttg cgtgtgcgca catccgtata
    43801 acgaatggtg ccttccagcc atgggaacag cgccacggag gtggagtaga accggtattg
    43861 atcgttatcc cggtagttga cgctgaattc accttccggt gcgatgcgcg catttggcat
    43921 ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa cccagcggat ccgggtaggt
    43981 taatacttca gcatgacagg cactggagac ggcaatggcc aggcagctta aaagataaga
    44041 attcttcttc atcagtcagg aacacgttgc gttaaaacag acacaatctg gtcatttaaa
    44101 tcagcatatt tttcgggcag tacatgcgca gaaaagccca accaaagctg gctgccaggc
    44161 ggtggttcca catgtctttt attccacagg gcaacagggg cgacgacagt ctcaccctca
    44221 ggcgtaatca ccataacgtt attcttgtcc gcgcctgcaa ggcgggggtg atcttgcaga
    44281 taatcggtaa cgctacggcc tgcctgccag ggcaattgcc cggccccaga aaccgcaccc
    44341 agcagagtaa tagtcaccgg acgctgtacg gtatagagcg tatagtcccc gaccaacggg
    44401 ggattgctgt tctcatccac tcgaacaaaa tcagggtcca gtttgaccgg taagcgacca
    44461 gtgatattga gattgagtaa ctgctggcgc acagatttaa tcgttgccgc cacatcgtcg
    44521 tcagcttctg cttcccagga agcaagctgt gccatgacat gttggtagtc tttaagcgct
    44581 ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg atcgcgcagt
    44641 tgcggttgtg tcaccaactg gacaacattt tccatggggc caacggaaag tgtctgttgt
    44701 tcgccaggca gataaatagt caccgttcct tgcgcaaagg catggggtgt cattacgtaa
    44761 agtacgctgg caatgaaata cgactgtaat ttattcatgg tgcgggttta agaaacgtca
    44821 tttctacggg aataacccct gcgcctaaca tttgccgact ttgacgaacc tggccagtag
    44881 ccgaatcgac ccagaatgtg ttttgccact gtttttctgg ccgcgtggaa gtcacctctt
    44941 cctgccagac attacacagc acattgcgac ccgcaatatt cagcgtctct aatccagcaa
    45001 aggaaaaagt agaactcagg gtcgcagagc gaaaatggtt gtcttcactc cagagaatat
    45061 cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt acgcagaggg tcctgcccgg
    45121 aatttgtcac ttccagcaga ttattattaa gtttgacggt ttttaaaaga cgtccattgt
    45181 gggtaaccag catggcgtta tcctgggata accatttgct ttgttcttgt tctatatatc
    45241 ccagtaccac aaagattcgt tgcccttcat taaggcgtaa atacatcgtg gaataaggca
    45301 acgcctggat ctgctgatcc gctacggtga tatcctgatt atcgaaaagg ctggcgcgaa
    45361 atgtatcgac catactttgc tggctgtgcg tacatcccgc aagaaatagt gcgaaaatcg
    45421 ataaaataag agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat gcaaatcttt
    45481 aaagcccggt acgaatatcg caaccgggta tcatttaact ttaatttcga cgcgttaaag
    45541 cagtattagc gtgtagtgga cgtggtggtt gaggtgttat gagaaccgcc atcgccactg
    45601 tcatttgctg caacaactgc caacgtcgcc acaccaacag ccgttgccac accgacaact
    45661 gtcgccgtgc cgacagcgcc agcagccacg ccaccagtgg tagtagcagt cgtggttgcg
    45721 ccggtagtgg tcgttgtggt gccagtagta cccgtcgttg cctcggtccc gtttgttgct
    45781 tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg ccataaggat tgcagaaagc
    45841 ttgtgtttca taacttttcc tttattcatc gcatggacaa tacgtacgct tgagatcagg
    45901 attgtctact ttgagtataa catccttgtg aaatctccct ttcctcatac cgcttgcact
    45961 atatactgcg ggtaggaaag gcgcgcagag ggaaatataa gattgttaac tatccccctc
    46021 tggaaatacg acttgtgagc ataagataaa tcttagcaaa atacaaatta aagataagga
    46081 aattgttacg aaagctatta attattgctg gtaatatagt ttcaagtggt actataactg
    46141 gctgttaaat ggcatttata actattaggt gcagagatat tcgcttaaat ggattagttt
    46201 ctttgtggaa aagtaactga tgttataata tatgatgttg actattgcac tattccaata
    46261 aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt tgccgctgtt
    46321 ggtcctcgca ggccatttac gcggcaaaat tccacacgta atcctggtat aagcacttct
    46381 gcgtcgcggg gagtgaatgc ggaaatatgg acctgaactt ctttacgacc gtcggagggg
    46441 ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt cattttacgg
    46501 gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa taaaataaag
    46561 ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct caattatcat
    46621 gcgttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt actaaagcag
    46681 ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac taagtagttt
    46741 ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat atgtctaata
    46801 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
    46861 atgatggcag caaagacgtt ttcgtccatt tcactgccat ccagagcaat gaattccgca
    46921 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
    46981 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
    47041 ccatgttgtc atggttcaca gtaccgcaca tcggcattcg atgtgacgga gcgaaatcct
    47101 ttgggcgcta agtgtatttt ttgtaaatcg acgatgatca cctttgataa cgtcacgctg
    47161 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcg
//
LOCUS       DADLTD010000004        43886 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLTD010000004 DADLTD010000000
VERSION     DADLTD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.004"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            83..625
                     /gene="lpfE"
                     /locus_tag="JN701_000169"
     CDS             83..625
                     /gene="lpfE"
                     /locus_tag="JN701_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HAY7362725.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPIPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYSTNNFTS"
     gene            865..2556
                     /gene="eptB"
                     /locus_tag="JN701_000170"
     CDS             865..2556
                     /gene="eptB"
                     /locus_tag="JN701_000170"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY7362726.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2648..2724
                     /locus_tag="JN701_000171"
     tRNA            2648..2724
                     /locus_tag="JN701_000171"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2682..2684,aa:Pro,seq:cgg)
     gene            3495..3725
                     /locus_tag="JN701_000172"
     CDS             3495..3725
                     /locus_tag="JN701_000172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362727.1"
                     /translation="MLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNPQLFTSGTTY
                     DASSVPLYNRLVEFKIGTTEVIPGLAEKWEVS"
     gene            3735..5075
                     /locus_tag="JN701_000173"
     CDS             3735..5075
                     /locus_tag="JN701_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7362728.1"
                     /translation="MYTFHLRKGVKWHDNKEFKPTRELNADDVVFSFDRQKNAQNPYH
                     KVSGGSYEYFEGMGLPELISEVKKVDDNTVQFVLTRPEAPFLADLAMDFASILSKEYA
                     DAMMKAGTPEKLDLNPIGTGPFQLQQYQKDSRIRYKAFDGYWGTKPQIDTLVFSITPD
                     ASVRYAKLQKNECQVMPYPNPADIARMKQDKSINLMEMPGLNVGYLSYNVQKKPLDDV
                     KVRQALTYAVNKDAIIKAVYQGAGVSAKNLIPPTMWGYNDDVQDYTYDPEKAKALLKE
                     AGLEKGFSIDLWAMPVQRPYNPNARCMAEMIQADWAKVGVQAKIVTYEWGEYLKRAKD
                     GEHQTVMMGWTGDNGDPDNFFATLFSCAASEQGSNYSKWCYKPFEDLIQPARATDDHN
                     KRVELYKQSQVVMHDQAPALIIAHSTVFEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            5383..6402
                     /gene="dppB"
                     /locus_tag="JN701_000174"
     CDS             5383..6402
                     /gene="dppB"
                     /locus_tag="JN701_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY7362729.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6412..7315
                     /gene="dppC"
                     /locus_tag="JN701_000175"
                     /pseudo
     CDS             6412..7315
                     /gene="dppC"
                     /locus_tag="JN701_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
     gene            7326..8309
                     /gene="dppD"
                     /locus_tag="JN701_000176"
     CDS             7326..8309
                     /gene="dppD"
                     /locus_tag="JN701_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY7362730.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8306..9310
                     /gene="dppF"
                     /locus_tag="JN701_000177"
     CDS             8306..9310
                     /gene="dppF"
                     /locus_tag="JN701_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY7362731.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEYIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9340..10611)
                     /locus_tag="JN701_000178"
     CDS             complement(9340..10611)
                     /locus_tag="JN701_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7362732.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11087..11194
                     /locus_tag="JN701_000179"
     CDS             11087..11194
                     /locus_tag="JN701_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY7362733.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11281..12960)
                     /gene="bcsG"
                     /locus_tag="JN701_000180"
     CDS             complement(11281..12960)
                     /gene="bcsG"
                     /locus_tag="JN701_000180"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY7362734.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12957..13148)
                     /gene="bcsF"
                     /locus_tag="JN701_000181"
     CDS             complement(12957..13148)
                     /gene="bcsF"
                     /locus_tag="JN701_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY7362735.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13145..14716)
                     /gene="bcsE"
                     /locus_tag="JN701_000182"
     CDS             complement(13145..14716)
                     /gene="bcsE"
                     /locus_tag="JN701_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7362736.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14989..15177
                     /locus_tag="JN701_000183"
     CDS             14989..15177
                     /locus_tag="JN701_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362737.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15189..15941
                     /gene="bcsQ"
                     /locus_tag="JN701_000184"
     CDS             15189..15941
                     /gene="bcsQ"
                     /locus_tag="JN701_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY7362738.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQKLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15938..18556
                     /gene="bcsA"
                     /locus_tag="JN701_000185"
                     /pseudo
     CDS             15938..18556
                     /gene="bcsA"
                     /locus_tag="JN701_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            18567..20906
                     /gene="bcsB"
                     /locus_tag="JN701_000186"
     CDS             18567..20906
                     /gene="bcsB"
                     /locus_tag="JN701_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY7362739.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYMPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPSIVPDESDRAAETRSTLTSSGAMATVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            20913..22019
                     /gene="bcsZ"
                     /locus_tag="JN701_000187"
     CDS             20913..22019
                     /gene="bcsZ"
                     /locus_tag="JN701_000187"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY7362740.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            22001..25474
                     /gene="bcsC"
                     /locus_tag="JN701_000188"
                     /pseudo
     CDS             22001..25474
                     /gene="bcsC"
                     /locus_tag="JN701_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25556..27544
                     /gene="hmsP"
                     /locus_tag="JN701_000189"
     CDS             25556..27544
                     /gene="hmsP"
                     /locus_tag="JN701_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY7362741.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLCPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27727..29013
                     /locus_tag="JN701_000190"
     CDS             27727..29013
                     /locus_tag="JN701_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY7362742.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29233..30729
                     /locus_tag="JN701_000191"
     CDS             29233..30729
                     /locus_tag="JN701_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7362743.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30825..31973)
                     /locus_tag="JN701_000192"
     CDS             complement(30825..31973)
                     /locus_tag="JN701_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7362744.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNIVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRANGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31986..32753
                     /gene="pdeH"
                     /locus_tag="JN701_000193"
     CDS             31986..32753
                     /gene="pdeH"
                     /locus_tag="JN701_000193"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY7362745.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32823..34883
                     /locus_tag="JN701_000194"
     CDS             32823..34883
                     /locus_tag="JN701_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7362746.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35117..36439)
                     /locus_tag="JN701_000195"
     CDS             complement(35117..36439)
                     /locus_tag="JN701_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7362747.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36850..37863)
                     /gene="yhjD"
                     /locus_tag="JN701_000196"
     CDS             complement(36850..37863)
                     /gene="yhjD"
                     /locus_tag="JN701_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY7362748.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(37912..38883)
                     /locus_tag="JN701_000197"
     CDS             complement(37912..38883)
                     /locus_tag="JN701_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7362749.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNE
                     LDGLFQQDATSISGKLRIDIPPGIAKNLLLPRLSEFLYLHPGIELELSSHDRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            39331..39933
                     /locus_tag="JN701_000198"
     CDS             39331..39933
                     /locus_tag="JN701_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7362750.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39984..41633)
                     /gene="treF"
                     /locus_tag="JN701_000199"
     CDS             complement(39984..41633)
                     /gene="treF"
                     /locus_tag="JN701_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY7362751.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42037..43434
                     /gene="ccp"
                     /locus_tag="JN701_000200"
     CDS             42037..43434
                     /gene="ccp"
                     /locus_tag="JN701_000200"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY7362752.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43645..>43886
                     /locus_tag="JN701_000201"
     CDS             43645..>43886
                     /locus_tag="JN701_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7362753.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10544 a        11000 c        11923 g        10419 t
ORIGIN      
        1 ccgttagcac taccggaaaa ttacccgctg ccggggatta tagcggtatt gctaccatac
       61 atgtcgaact ggagtaacag tcatgaagcg aacaatactt gcgttatgcc tgctgcctct
      121 ttccttttcg tcattcgcgg caaccacgga tttagggccg aaaggaacat tgaaattcac
      181 ccttaaaatc agccagggtg cttgcgaact ggtgaaagac agcgttgagg ttgacatggg
      241 caccgcggtt ctcaaaaagc cggtaagaat cggaacggaa attaatccca ctcctttttc
      301 catcggcctg aaaaactgta gcgaagtggt cagagcctac gtgactatgg acggtacacc
      361 caatgcagac gactcaaatc tttttgcgct ggatgcgggt ggcgctacag gcgttgggct
      421 gaaaatcaaa accgctgctg gagtacaaca gttcccggaa aatacaacgc cgataccggt
      481 tgagtttgca attaacggtg atggcgcaca tcaactgaac tatatcgcca gctacgtgcc
      541 tattcgcgcc gatgcaacta ttggtcgcgc agatgcaacg gtcgacttta ctgtacagta
      601 cagtacgaat aatttcacat cttaagggcc agttaactgg ccctttttac tgctttttgt
      661 aacgctaaag tctcttttca aacttgcatt tttgtaaatt tgtgcttcat gcacactctt
      721 tccccacact ttttcgcttt gctgtggtct acttattcgc gcgtgtagat tttacttatc
      781 tgactacctc cgcacttttc ccctgccggg cctgaaaagc cgctaagcag ggtgttatca
      841 cctgtttgtc cagggtttgt ttgcatgaga tacatcaaat cgattacaca gcagaagctg
      901 agctttttgc ttgcaatcta tattggcctt tttatgaatg gcgcggtttt ttatcgccgc
      961 ttcggcagct atgcgcacga ttttaccgtc tggaaaggcg tttctgctgt tgttgaactg
     1021 gccgccaccg tactggtgac cttcttttta ctacgtcttc tatcgctgtt tggccgccgc
     1081 agctggcgta ttctggcgtc gctggtggtg ctcttttccg caggtgccag ctattacatg
     1141 accttcctta atgtggtcat tggttatggc atcatcgctt ccgtcatgac caccgatatc
     1201 gacctgtcaa aagaagttgt tggtctgaac tttattctct ggttaatcgc cgttagtgca
     1261 ttgcctctta tccttatctg gaataaccgc tgtcgctaca ccttgctccg acaactgcga
     1321 accccagggc agcgtattcg cagcctggcg gtcgtcgtac tggcgggtat tatggtttgg
     1381 gcaccgattc gtttgctgga tatccagcag aagaaagtgg agagggcgac cggcgttgat
     1441 ttgccgagtt atggcggtgt cgtagcgaac tcttatctgc catcaaactg gctttctgcg
     1501 ttggggctgt atgcctgggc gcgggtcgat gaatcttccg ataataattc attgcttaat
     1561 ccggcgaaga agtttacata ccaggctcca caaaacgttg atgatactta tgtcgtgttt
     1621 atcatcggtg aaaccacgcg ttgggaccat atgggtattt tcggctatga gcgtaatacc
     1681 acgccgaaac tggcccagga gaaaaatctg gcggcgttcc gtggttactc ctgtgatacc
     1741 gcaaccaaac tctcactgcg ttgcatgttt gtacgtcagg ggggcgcgga agataatccg
     1801 cagcgcacat taaaagaaca gaacattttc gcggttctga agcagttagg attcagttct
     1861 gacctctacg ctatgcagag cgaaatgtgg ttctacagca acacgatggc ggacaacatt
     1921 gcttatcgtg agcagattgg tgcggagcca cgtaatcgtg gcaagccggt agatgatatg
     1981 ttgctggtag acgaaatgca gcaatcgcta gggcgcaacc cggatggtaa gcatctgatc
     2041 attctgcata ccaaaggttc gcattttaac tacacccagc gttatccgcg tagcttcgcg
     2101 cagtggaagc cggaatgtat tggtgttgat agcggctgta ccaaagcgca gatgatcaac
     2161 tcctatgaca actcggtgac ctatgtggat cactttatct ccagcgtgat tgatcaggtt
     2221 cgcgataaga aagcgattgt gttctacgca gctgaccacg gtgagtcaat taatgaacgc
     2281 gagcacctgc acggcacgcc gcgtgaattg gcaccgccgg agcagttccg cgtaccgatg
     2341 atggtctgga tgtcagataa atatctggaa aatccggcca atgcgcaggc atttgcgcag
     2401 ctgaaaaaag aagccgacat gaaagtgcca cgccgtcacg tagagctgta cgataccatc
     2461 atgggttgtc ttggctatac ttcaccggat ggtggaatta acgaaaacaa caactggtgt
     2521 cacatcccgc aggcaaaaga ggcagcggct aactaagcgg cctgctgact ttctcgccga
     2581 tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt aagatgcgcc
     2641 ccgcattcgg tgattggcgc agcctggtag cgcacttcgt tcgggacgaa ggggtcggag
     2701 gttcgaatcc tctatcaccg accaaattcg aaaagcctgc tcagcgagca ggcttttttg
     2761 catttgaagc ccatgaggat gagaacctcc gggggctggg aggttcgact cgacgtaggc
     2821 ctgataagac gcgccagcgt cgcatcaggc aacggcaccg cacttattgt cggatgcggc
     2881 gcgaccgcct tattcgacca acacaccaca cattttcctg ccaactttgc gccccatatc
     2941 tcactatcca ttaacggatt tgtgacagaa accattgtct ttttttatat ttgcttaaat
     3001 ctttttttgc gttacttatg gctggcgctg gcattttccg ctgtgcgttt tagcgttcat
     3061 ggtattaatc gctcagatat ccaccttttc ctcagatttt tttcattaaa tgcataaaac
     3121 ttaatcacta aatgttgcta aatattaagc tgattgttct ggttatgttg gagtagcaca
     3181 aatttctctg ctgcaaatag ctgtttgaag ttttttaatc tttgtttgtg ggttctcacc
     3241 gttggtgtaa atcccggttg gctgtattga cgttttcaca ttctgttgac agattgtagg
     3301 tcacgagggg cattttatgg aggatccgca ctgttacact gatgttaatt agtacggcat
     3361 ccccacctca taacgttgac ccgaccgggc aaaaaacaaa aaaggtcagg tagcgacaac
     3421 ccactgcaaa gggttaaaac aacaaacatc acaattggag cagaataatg cgtatttcct
     3481 tgaaaaagtc agggatgctg aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa
     3541 gtgttcaggc taaaactctg gtttattgct cagaaggctc tccggaaggg tttaacccgc
     3601 agctgtttac ctccggcacc acctatgacg cctcttccgt accgctttat aaccgcctgg
     3661 ttgaatttaa aatcggcacc actgaagtga taccgggcct cgctgaaaag tgggaagtca
     3721 gctaagacgg taaaatctat accttccatc tgcgtaaagg tgtgaagtgg cacgacaata
     3781 aagaattcaa accgacgcgt gaactgaacg ccgatgacgt ggtgttctcg ttcgaccgtc
     3841 agaaaaacgc gcaaaacccg taccataaag tctctggcgg cagctacgaa tacttcgaag
     3901 gcatgggctt gccggagctg atcagtgaag tgaaaaaggt ggacgacaac acagttcagt
     3961 tcgtactgac tcgcccggaa gcgccgttcc tcgctgatct ggcaatggac ttcgcctcta
     4021 ttctgtcaaa agaatatgct gatgcgatga tgaaagccgg tacaccggaa aaactggacc
     4081 tcaacccaat cggaaccggt ccgttccagt tacagcagta ccaaaaagat tcccgtatcc
     4141 gctacaaagc gtttgatggc tactggggca ccaaaccgca gatcgatacg ctggtcttct
     4201 ctattacccc tgatgcttcc gtgcgttacg cgaaattgca gaagaatgaa tgccaggtga
     4261 tgccgtaccc gaacccggca gatatcgctc gcatgaagca ggataaatcc atcaacctga
     4321 tggaaatgcc ggggctgaac gtcggctacc tctcgtataa cgtgcagaaa aaaccgctgg
     4381 atgacgtgaa agttcgccag gcactgacct acgcggtgaa caaagacgcg atcatcaaag
     4441 cggtttatca gggcgcgggt gtatcagcga aaaacctgat cccgccaacc atgtggggct
     4501 ataacgacga cgttcaggat tacacctacg atcctgaaaa agcgaaagcc ttgctgaaag
     4561 aagcgggtct ggaaaaaggt ttctccatcg acctgtgggc gatgccggta caacgtccgt
     4621 ataacccgaa cgctcgctgc atggcggaga tgattcaggc agactgggcg aaagtgggcg
     4681 tgcaggccaa aatcgtcacc tacgaatggg gtgagtacct caagcgtgcg aaagatggcg
     4741 aacatcagac ggtaatgatg ggctggactg gcgataacgg ggacccggat aacttcttcg
     4801 ccaccctgtt cagctgcgcc gcctctgaac aaggttctaa ctactcaaaa tggtgctaca
     4861 aaccgtttga agatctgatt caaccggcgc gtgctaccga cgaccataac aaacgcgttg
     4921 aactgtacaa gcaatcgcag gtggtgatgc acgatcaggc tccggcactg atcatcgctc
     4981 actccaccgt gtttgaaccg gtacgcaaag aagtcaaagg ctatgtggtt gatccattag
     5041 gcaaacatca cttcgaaaat gtctctatcg aataattaaa agccatacaa gactgatggt
     5101 aaaggcaaaa ctgcctgatg tgctccgctt atcaggccta cgaaaattct gcaatgtatt
     5161 gaatttgcac gattttgtag gccggataag gcgttaacgc cgcatccggc ataaacaaag
     5221 cgcactttgt caacaatctg tatacccggt ggcgctgtgc ctctgcgtgc agcgccatcc
     5281 ggcagcaata cttcattctc tgtccgacaa gggcgggaat tgatttgtga gcaatacaga
     5341 cacgcagttc caggctgcgg gtcactatag agaatccggg ttatgttgca gtttattctc
     5401 cgacgtttgg gactcgtcat ccccacgttt atcggtatta cccttctcac atttgccttt
     5461 gtccacatga tcccgggcga tccggtgatg atcatggcag gcgaacgtgg gatctcccct
     5521 gagcgtcacg cgcagctgct ggctgaactc ggcttagata aaccgatgtg gcagcagtat
     5581 ctccattaca tttggggcgt aatgcacggc gatctaggca tttcaatgaa aagccgaatt
     5641 ccagtatggg aagagttcgt gccgcgcttt caggccacgc tggaacttgg cgtctgcgcg
     5701 atgatttttg ctacggcagt cggtattccg gttggtgtgc tggctgcggt taaacgcggt
     5761 tccattttcg atcacactgc agttggcctg gcgctgaccg gctactcgat gccgatcttc
     5821 tggtggggca tgatgctgat catgctggtt tcggtgcact ggaacctgac gcccgcctcc
     5881 ggtcgcgtga gcgatatggt gttcctcgat gactccaatc cgttaaccgg ttttatgcta
     5941 atcgacaccg ccatctgggg tgaagacggc aactttatcg atgccgtcgc ccatatgatc
     6001 ttgcctgcca ttgtgctggg tactattccg ctggcggtca ttgtgcgtat gacacgctcc
     6061 tcgatgctgg aagtgctggg cgaggattac atccgcaccg cgcgcgccaa agggctaacc
     6121 cgcatgcggg tgattatcgt ccatgcgctg cgtaacgcga tgctgccggt ggtgaccgtt
     6181 atcggcctgc aggtgggaac attgctggcg ggggcgattc tgaccgaaac catcttctcg
     6241 tggcccggtc tgggacgctg gttgattgac gcactgcaac gccgcgacta tccggtagtg
     6301 cagggcggcg tattgctggt ggcgacgatg attatcctcg tcaacttgct ggtcgatctg
     6361 ctgtacggcg tggtgaaccc gcgtattcgt cataagaagt aaggggacat catgtcacag
     6421 gttactgaaa ataaagtgat tagcgcaccg gtgccgatga ccccgttaca ggagttctgg
     6481 cactatttta aacgcaacaa aggcgcggtc gtcgggctgg tttacgtcgt catcgtgctg
     6541 ttcatcgcga tctttgccaa ctggattgca ccctataacc cggcggaaca gttccgcgat
     6601 gcactgctcg ccccgccagc ctggcaggaa ggcggcagca tggcgcactt gctgggcacc
     6661 gatgacgtag gccgtgatat gctgtcgcgc ctgatgtacg gtgcgcgcct gtcgctgctg
     6721 gttggctgtc tggtagttgt gttatcgctg attatgggcg ttattctcgg cctgatcgcc
     6781 ggttactttg gcggcctagt cgataacatc attatgcgcg tggtcgatat catgctggcg
     6841 ctgccaagtc tgctgctggc gctggtgctg gtggcaattt tcggcccgtc gattggtaac
     6901 gccgcgctgg cactgacctt cgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc
     6961 gtgctggtgg aagttaaccg cgattacgtc accgcgtctc gcgtggcggg tgccggggcg
     7021 atgcgtcaga tgtttattaa catcttcccg aactgccttg cgccgctgat tgttcaggtg
     7081 tcgctcggtt tctctaacgc cattctcgat atggctgctc ttggtttcct cggcatgggg
     7141 gcacagccgc caacgccgga gtggggcacc atgctctccg acgtgttgca gttcgcgcaa
     7201 agcgcctggt gggtcgtgac cttcccgggt ctggcgatcc tgctgacggt gctggcattt
     7261 aacctgatgg gtgacggtct gcgatgacgc gctcgatccc aaactgaagc agtaagaggt
     7321 tcgagatggc gttattaaat gtagataaat tatcggtgca tttcggcgac gaaagcgcac
     7381 cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca gggcgaagtg gttgggattg
     7441 tgggtgagtc cggctccggt aagtcggtca gttccctggc gattatgggg ctgattgatt
     7501 atccgggccg cgtaatggcg gaaaaactgg tgtttaacgg tcaggatttg cagcgtatat
     7561 cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc gatgatcttc caggacccga
     7621 tgaccagcct taacccgtgc tacaccgtgg gtttccagat catggaagcg attaaggtgc
     7681 atcagggcgg caacaaaagt acccgccgtc agcgggcgat tgacctgctg aatcaggtcg
     7741 gtattcccga tccggcttcg cgtctggatg tttacccgca tcagctttcc ggcggcatga
     7801 gccagcgcgt gatgatcgcc atggcgattg cctgtcgacc aaaactgctg attgccgatg
     7861 agccgaccac cgcgctggac gtgaccattc aggcgcaaat catcgaacta ctgctggagc
     7921 tacagcagaa agagaacatg gcgctggtgt tgattaccca tgacctggcg ctggtggcgg
     7981 aagctgcgca taaaatcatt gtgatgtatg caggccaggt ggtggaaacg ggcgatgcgc
     8041 acgccatctt ccatgcgccg cgtcacccgt atactcaggc attgctgcgt gcgctgccag
     8101 aatttgctca ggacaaagaa cgtctggcgt cgttgccagg tgtcgttccc ggcaagtacg
     8161 accgcccgaa cggctgcctg cttaacccgc gctgccccta tgccactgac agatgtcgcg
     8221 ctgaagaacc ggcgctgaat atgctcgctg acgggcgtca gtccaaatgc cattacccac
     8281 ttgatgatgc cgggaggccg acactatgag tacgcaagag gccaccctgc aacaaccgct
     8341 gttgcaggct atcgacctga aaaaacatta tccggtgaag aaaggcatgt tcgcgccgga
     8401 acgtctggtt aaagcgctgg atggcgtttc gtttaacctt gaacgtggca aaacgctggc
     8461 agtagtgggc gaatctggct gcggtaaatc gaccctcggt cggttgctga cgatgattga
     8521 aacgcccacc ggtggcgagc tgtattacca ggggcaggat ctgcttaagc acgatccgca
     8581 ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc cagaaccctt acggttcgct
     8641 gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg ctgctgatca acaccagctt
     8701 aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg gcgaaagtcg gcctgaaaac
     8761 cgagcactat gaccgctatc cgcatatgtt ctccggcggt cagcgtcagc gtatcgccat
     8821 cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc gatgaaccgg tttccgcgct
     8881 ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg gatttgcagc aggagttggg
     8941 gctgtcttat gtctttatct cccacgacct gtcggtggtg gagtacattg ctgatgaagt
     9001 gatggtgatg tacctgggcc gctgcgtgga gaagggaacg aaagaccaaa tcttcaataa
     9061 cccgcgccat ccgtacactc aggcgctact ttccgcgacg ccgcgcctga acccggacga
     9121 tcgccgcgag cgcatcaagc tcagcggtga actaccaagc ccactgaatc caccgccggg
     9181 ttgcgccttc aacgctcgct gtcgtcggcg cttcggcccc tgcacccagt tgcagccgca
     9241 gctaaaagac tacggcggtc aactggtagc ttgttttgct gttgatcagg atgaaaatcc
     9301 gcagcgttaa ctcacaaaac cggggccatg cgctccggtt tactcaatca gcttcaacag
     9361 tggcgacaag catagcaaaa tgccgtaaag cagaattagc caggctttaa atccgcgaag
     9421 tttttccagt tgtgcgactt tatagatcag gaaaaacgga atgaggcacg acacaatgcc
     9481 atataacggg cttcccaact gaaagaacac cagcaccgat acacgaaacg aaacccaaat
     9541 cgtcaacgta atgacgataa aagcgcagat cgccagagtc agcatgcgtg agttaatttt
     9601 cttggtatca ataattcggc ttaacagatt gagaataatg cctttaatag cctcgtggaa
     9661 accgagataa atgccaaaga atgcggtcag tacggcaaag atattcagca ccgtagaggt
     9721 gatatgaatg atatgtccag ggatcacctg cgcggccagt gccagtgccg agatattttg
     9781 ttcaaaggca gaaacggctt cttcgtggct aattgagaag gtaaacgaaa aggcaaaaaa
     9841 caggatcacc gcgatgagcg tgatataact aattcggtgg gtacgcagcg cgagccgggt
     9901 tgccagtact ttatctgctt cccgtttacg ataggcaata ttcattgggt taagtacctg
     9961 aataaatacc gcagaaaaga agcaaaatgg aattgtaagc aggacatcgc ggaaaaagac
    10021 ggaggcttgc gggaaggcgg ttatattggc gaaattccag tgcgggatca tcgcaaaacc
    10081 gaacacgaca ataatcccta ctttgaccac caccattggc ccggaaatct taaacagtaa
    10141 tcgttcacca ccagacgcaa tcgctaccag tacggcgaaa atagcgactt tataaagtag
    10201 agattgtgaa agatcggcat cggttaaacc gaaggttttc aggtacgagg cgctgtcgaa
    10261 aaccacggag agagagtaga taaaaatccc gtggataatc atcaaaaagt agataacccc
    10321 gaggaaaatt ccccagttct tccccaggta atgactgata atatcggtgt agtcattaca
    10381 ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc actacccagg tggcaggata
    10441 agcaatgatc gctgcggtaa taaataccca aattcccttc aagccaattt gtactggcat
    10501 cagcacggtt ccggcaccaa tcgccatgcc tatgcataat aaaacccagc cgaagtcgta
    10561 gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg ttgtgctgca taaagtcctg
    10621 tccagaaaga agtcggcctc gcaaaaatga ataaacccag tttctcttgc gcaacaggtg
    10681 gagaagtcga atacgcagta tgtaaaaaat gaggcaacag attcactggg gaggtcgcga
    10741 ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt ctggagtcag cccggatatg
    10801 aactgcaact attgcaaata aagaaattaa gctggaaggc atcgcgcaat gaggaagatt
    10861 gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg caagccgaaa gggttccggt
    10921 gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc
    10981 cccgcacgtt gcaggtatgc acctgacaac atcacggggg ctaatcttga acccaaacaa
    11041 ctcaagaata gccgcgaaac gttggcatta caacacaggc ggctatatga cgctcgcaga
    11101 actgggcatg gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag
    11161 tatgatcgtg aactggctga ataagcggaa gtaacgtgtc atgcgggcgt caggctgccg
    11221 taatggcaat ttgcgcccgg atcaggccgc agaggttaaa ctctgcggcc tttttcgtgc
    11281 ttactgcggg taaggcaccc agtcgccgcc gttcaggcga acgtacggtt tatcctggta
    11341 ttgaataact actgcatttg agttctcgga gaccggtgct gtttgtggca acccactggt
    11401 gagttttttc cagtcaacat tgtcttcggt gaaaatcttg ccatcaagaa cgcgaaccac
    11461 cagatcggag atagccagga agctgctcgg ttggtcgatg acaatcggtg ccccctgatg
    11521 tggtgccttc atgccgaaga atttcacccc aacggggacg tctgtgatag acgggctagg
    11581 gatatcacgt aggccagata cctgcattct gtcgcccttc agcgcgccgc cgtgttccgg
    11641 caccacgacc accatcactt tacgacccga tttctccagt tcagtaaaga aggcgtccag
    11701 ttcatcaaag aatttctgcg cccgcgcttt gtaatccgct gttttgctga cccccggata
    11761 atggttgccg tcatgcagtg gaagcgtgtt gtagaacgtg gcactacggc tgtttttatc
    11821 tttttcggta acgtccagcc agcggttaag cacggtggta tcgtcataaa ccggcgaacc
    11881 atcaaagccc agcaaaataa ccggcagatt tgtttgatcc atcaattcag tctgcatgcc
    11941 gccattttcg cgaacttctt tcaaaaaacc gccgaactgg ccgttatgtc ccatcatcag
    12001 gtgctgggta aagcccagtt tcgaaagatt atcaaacaga tagcagtcgt tatttgccgg
    12061 ttgatacaga ttagtgtgcg aagtctgccc gcagctggcg cgcagtaaac ggatcgccgc
    12121 cgggccactg taggaggtgg cggagttaaa gttcttgaac tcaatatcga aatgcgacca
    12181 cagtggatgc gacatcaacc cggcggcttc tatatccgac caggaaagcg aacagatgtt
    12241 aatcaccagt agttcaaatg gctgagcatc agcgggcagc gaagacggga aggtcgattt
    12301 acgtttcgcc tccgcgttat agaaattatt cagccaggcg ttaaggttcg ccgttgttgg
    12361 cggtgtagtt tgtgcgggca tatcacccac taccggtgcg ccacccgtcg tctcaacggt
    12421 tgccgctgcg ttaccacccg tcgttgttac agtggtcgtc ggttgtccgg ctggccacaa
    12481 ggagaaactt ggtcccgcca gggtaagtac gttcagccat agcagtatgg caaccacaaa
    12541 aacggtaatg cgaatccatt gtgacaggaa taaccaggcc actaataaaa caaaaatggc
    12601 cccaatcatc tgccagttaa taaagcgtgt gacaaggtcg attaaataat cggtactgaa
    12661 ccccgccacc tgcgaaccct ggctcattat gctttccggg ccaggcaacc aggtgtcatg
    12721 ccagaacaaa gcaaagccga tcggcagggc aatccagtgg cgcaagcgat gcaggctgta
    12781 gcgcggaatg ggcatcagca gaaacgcggc aaacaccaaa ttgaggagcg gatggaagtt
    12841 aagatatccc gcccacaaca ggccgaactt aaccagaaaa tagaagttcc agccggaaag
    12901 gccgcgccag tattgccaga gggaagaagg catggcggta ttttgcgtaa attgagtcat
    12961 tttttggttg ccctggcttt ttccgtgcgg cgtaatgtcc cggccggttt aacataacga
    13021 ggtttagcaa agaacaaacg taaggtgtcg cgaatgcgtc gcaaagagtg ccgcgccaga
    13081 tagcccagcg ggaaaaatat cagtgcgcaa acgacaataa tttcaatgat atcgctgatg
    13141 gtcatcatga tgagcgctcc acagcatcat ctaacagtcg catgggttct ggtattcgtc
    13201 gccagtggcg accatcgtgc tcggcattga tgaccggttt agaactttgc gttaaaggca
    13261 gcggcatgcc ccattgttct ggggcaagca ggcgcatctg caccagctcg gcactgattt
    13321 gatcatcttc aaaccagacc atacggtttg agaaaatgtc gccagtaggc aatgggaaaa
    13381 tatgattcaa cgcggtatcc agatcgttaa tccgacagaa tgagagaaac agcaccagcc
    13441 gattaccgcc aatggtcatg atatcgccgg tacggttagg gcgacacagc gtcagggctt
    13501 gttcaacgcg gatacccggt accggacgta gggcaaccag aacgccttta ccgtgggcag
    13561 gtaatagagg gttattcatc atgttgttga cggcattaca gaacacatcc cacttctgga
    13621 aaccacgcag tttgagcggc tgggtcattg acagcaaggt agtgatatct tccggcacat
    13681 agcgactaaa cttctgccct tgcacgcttt cgatcatcgt cagacaacgg gagagtggcg
    13741 cattccacgg aataaccata tttgcaccgc aggccaataa caaacgttca tcggtggcgc
    13801 gcaggctagc ggtattttcc cgcacgagga ttttcatcgc actaccgcgc tggcgacgca
    13861 gggtatgaat gctgcgggcc agtggctcga tttgcgcatt ttgctgtaaa gaaaagacca
    13921 ccgtcgccgc ctgagcggta cgggcttcat tgaacaggac ttcgttattg ttgaacagtt
    13981 gccagtgttc cgatagcggc ggcgcacctt ccagtacagc aacattactc agaatgcgtt
    14041 tttcgtcgct gcgtggttgg atctccgcct cttcgctttg aactaatgtc cagataccat
    14101 tttgttgctg aacgctaagc tgctgacggg cgctgacccc tttttcgttg caccagaagg
    14161 caatatccag caaatgttgg tcaccctgaa aacgcaaact ggcaagacca aaaagtgaac
    14221 ggtattcctc aagcaacaat gaaaattgtt tatcgttatt atttccggga ttaattacca
    14281 aaagcgaaca atgggttaac ctgctccatt tattcatttt atccaaccat gagcgaagcc
    14341 gctcggcagg aatgttttgc catgcgttat ttgcgcaaac aagaataaaa aggtaattat
    14401 ggggatcaat agaacactgc aaatcacggg tcaaatagta tagacctttt tcatgattta
    14461 gcattgaaaa taattttatt ttttccggcc cttgataatc atctaattga aagattttcg
    14521 ccggatcgct gtccatgcta atgaccgcga cgtgtgcggt ttcagcctgg gatgcaattg
    14581 tttgattcgc cagactgata gcatcttcat ggcgatcgac gttaaaccac cagacgccgc
    14641 ctgctggcat atgtcgcagc tcatcccata atgatgagat accgatagag aatacagggc
    14701 ccacaatgtc cctcatgtca ccattttgtc tgattatcag tttactagcg aaagctaaga
    14761 aataaaccta ccattgaaat taaaacttat cagatttagc atgtgaatca atttagaagg
    14821 tcacaagacg tgtgatttta gtcttttcgt ttttaacttt ttctgttgcg taacggaaag
    14881 tcaaaaagtg agcaaattcc cgtctcgccg taaacaataa accggcgaaa tgctcacggt
    14941 tagaattgtc tcatgaacgg tacggttatt tcatagggat caagcaaaat gaataacaat
    15001 gaaccagata ctctgcctga tcccgcgata ggctatatct tccagaatga tattgtggcg
    15061 ttaaagcagg cattttcact gcctgatatt gattatgccg atatttccca acgcgaacag
    15121 ttggccgcgg cattaaaacg ctggccgttg ctggcagagt ttgcgcaaca aaagtagggg
    15181 attggtgaat ggccgtactg ggattgcagg gggtgcgggg aggcgtgggg acaacaacca
    15241 tcaccgccgc attagcctgg tcattacaaa tgttgggaga aaatgtcctg gtggtcgatg
    15301 cctgcccgga caacttgttg cgcctgtcat ttaatgttga ttttacccac cgtcagggct
    15361 gggccagagc gatgctggat ggccaggact ggcgtgacgc tgggttgcgc tacacctcgc
    15421 agctcgattt gctgcctttt ggtcagttat ccattgaaga acaagaaaat ccacagcaca
    15481 ggcaaacccg gctgagcgat atttgctccg gcttacagaa actaaaagcc agcgggcgtt
    15541 accagtggat tttaatcgac ttaccgcgtg atgcctcgca gataacccac cagctgctga
    15601 gtttgtgcga tcactcgctg gcaatcgtca atgtggatgc caactgccat atccgactgc
    15661 atcagcaagc gctgccggat ggcgcacata ttttgattaa tgacttccgt attggcagtc
    15721 aggttcagga cgatatttac cagctttggt tgcaaagcca gcgccgatta ctgccgatgc
    15781 tcattcatcg tgatgaagcg atggctgaat gcctggcggc taagcaacca gtaggtgaat
    15841 atcgcagtga tgcgctggcg gctgaagaga tactgacgct ggcgaactgg tgcctgttga
    15901 actactccgg gctgaaaacg ccagtcggga gtgcatcatg agtatcctga cccggtggtt
    15961 gcttatcccg ccggtcaacg cgcggcttat cgggcgttat cgcgattatc gtcgtcacgg
    16021 tgcgtcggct ttcagcgcga cgctcggctg tttctggatg atcctggcct ggatttttat
    16081 tccgctggag cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc tgtatccgca
    16141 tatcaacgcc tcgcgtccgc gtccgctgga cccggtccgt tatctcattc aaacatgctg
    16201 gttactgatc ggtacatcgc gcaaagaaac gccgaaaccg cgcaggcggg cattttcagg
    16261 tctgcagaat attcgtggac gttaccatca atggatgaac gagctgcctg agcgcgttag
    16321 ccataaaaca cagcatcttg atgagaaaaa agagctcggt catttgagtg ccggggcgcg
    16381 gcggttgatc ctcggtatca tcgtcacctt ctcgctgatt ctggcgttaa tctgcgttac
    16441 tcaaccgttt aacccgctgg cgcagtttat cttcctgatg ctgctgtggg gtgtagcgct
    16501 gatcgtacgg cggatgccgg ggcgcttctc ggcgctaatg ttgattgtgc tgtcgctgac
    16561 cgtttcttgc cgttatatct ggtggcgtta cacctctacg ctgaactggg acgatccggt
    16621 cagcctggtg tgcgggctta ttctgctctt cgctgaaacg tacgcgtgga ttgtgctggt
    16681 gctcggctac ttccaggtcg tatggccgct gaatcgtcag ccggtgccat tgccgaaaga
    16741 tatgtcgctg tggccgtcgg tggatatctt tgtcccgact tacaacgaag atctcaacgt
    16801 ggtgaaaaat accatttacg cctcgctggg tatcgactgg ccgaaagaca aactgaacat
    16861 ctggatcctc gatgacggcg gcagggaaga gtttcgccag tttgcgcaaa acgtgggggt
    16921 gaaatatatc gcccgcacca ctcatgaaca tgcgaaagcg ggcaacatca acaatgcgct
    16981 gaaatatgcc aaaggcgagt tcgtatcgat tttcgactgc gaccacgtac caacgcgatc
    17041 gttcctgcaa atgaccatgg gctggttcct gaaagagaaa cagctggcga tgatgcagac
    17101 gccgcaccac ttcttctcgc cggacccgtt tgaacgcaac ctggggcgtt ttcgtaaaac
    17161 gccgaacgaa ggcacgctgt tctatggtct ggtgcaggat ggcaacgata tgtgggacgc
    17221 cactttcttc tgcggttcct gtgcagtaat tcgtcgcaaa ccattggatg aaattggcgg
    17281 tattgctgtc gaaacggtaa ccgaagatgc gcacacgtct ctgcgcctgc accgtcgtgg
    17341 ctacacctct gcatatatgc gtattccgca ggcggcgggg ctggcgaccg aaagtctgtc
    17401 ggcgcatatc ggtcagcgta ttcgctgggc acgcgggatg gtgcaaatct tccgtctcga
    17461 taacccgctt accggtaaag ggctgaaatt cgcccagcgg ctgtgctacg tcaacgccat
    17521 gttccacttc ttgtcgggca ttccacgact gatcttcctg actgcgccgc tggcgttcct
    17581 gctgcttcat gcctacatca tctatgcgcc agcgttgatg atcgccctgt tcgtgctgcc
    17641 gcatatgatc catgccagcc tgaccaactc gaagatccag ggcaaatatc gccactcttt
    17701 ctggagtgaa atctacgaaa cggtgctggc gtagtatatc gcaccaccga cgctggtggc
    17761 gctgattaac ccgcacaaag gcaaatttaa cgtcaccgcc aaaggtggac tggtggaaga
    17821 agagtacgtc gactgggtga tctcgcggcc ctacatcttc cttgttctgc tcaacctggt
    17881 gggtgttgcg gtcggcatct ggcgctactt ctatggcccg ccaaccgaga tgctcaccgt
    17941 ggtcgtcagt atggtgtggg tgttctacaa cctgattgtt cttggcggcg cagttgcggt
    18001 atcggtagaa agcaaacagg tacgccgatc gcaccgcgtg gagatgacga tgcccgcggc
    18061 aattgcccgc gaagatggtc acctcttttc gtgtaccgtt caggatttct ccgacggtgg
    18121 tttggggatc aagatcaacg gtcaggcgca gattctggaa gggcagaaag tgaatctgtt
    18181 gcttaaacgc ggtcagcagg aatacgtctt cccgacccag gtggcgcgcg tgatgggtaa
    18241 tgaagttggg ctgaaattaa tgccgctcac cacccagcaa catatcgatt ttgtgcagtg
    18301 tacgtttgcc cgtgcggata catgggcgct ctggcaggac agctacccgg aagataagcc
    18361 gctggaaagt ctgctggata ttctgaagct cggcttccgt ggctaccgcc atctggcgga
    18421 gtttgcgcct tcttcggtga agggcatatt ccgtgtgctg acttctctgg tttcctgggt
    18481 tgtatcgttt attctgcgcc gcccggagcg gagcgaaacg gcacaaccat cggatcaggc
    18541 tttggctcaa caatgatgat aacgcgatga aaagaaaact attctggatt tgtgcagtgg
    18601 ctatggggat gagtgcgttc ccctctttca tgacgcaggc gacgccagca acgcaaccac
    18661 tgatcaatgc tgagccagct gtagccgccc agacggaaca aaatccgcag gtggggcaag
    18721 tgatgccggg cgtgcagggc gctgatgcgc cagtcgtggc gcagaacggt ccttcgcgtg
    18781 atgtgaagct gacctttgcg caaattgcgc ctccgccggg cagcatggtg ctacgtggca
    18841 ttaacccgaa cggcagcatt gagtttggta tgcgcagcga tgaagtggtg acgaaggcga
    18901 tgctcaacct cgaatacatg ccatcgccat cgttactgcc tgtccagtcg cagttaaagg
    18961 tttatctcaa tgatgagctg atgggcgtac tgccagtgac caaagaacag ttgggtaaaa
    19021 aaacgctggc gcaaatgccc attaacccac tgtttattac cgacttcaac cgtgtgcggc
    19081 tggagtttgt cggccattat caggacgtgt gcgaaaaccc ggccagcacc acgctttggc
    19141 tggatgttgg gcggagcagt ggactggatc tgacctatca gaccctgaat gtgaagaatg
    19201 acctgtcaca cttcccggtg ccattctttg acccgcgcga taaccgtact aacaccttgc
    19261 cgatggtctt tgcgggtgcg ccggatgttg ggctgcaaca agcatctgcc attgtcgcct
    19321 cgtggtttgg ttcgcgttct ggctggcgtg ggcagaactt cccggtgctc tataaccaac
    19381 tgccggatcg taatgccatt gtctttgcaa ccaacgacaa acggccggac ttcctgcgcg
    19441 atcatccggc ggtaaaagcc ccggtgattg agatgatcaa ccatccgcag aatccttacg
    19501 tcaaactgct ggtggtgttt ggtcgtgacg acaaagacct gttgcaggca gcgaaaggta
    19561 tcgctcaggg taacattctg ttccgtggtg aaagcgtggt agtgaatgaa gtgaaaccgc
    19621 tgctaccgcg taagccgtac gatgcgccga actgggtacg taccgatcgt ccggtcacat
    19681 ttggcgaact gaaaacctat gaagaacagt tacaatccag cggtcttgag ccagcagcga
    19741 ttaacgtttc gctaaacctg ccgccggatc tctacctgat gcgcagtacc ggcattgata
    19801 tggatattaa ttaccgctac accatgccgc cggtgaaaga cagttcgcgg atggatatca
    19861 gcctgaataa ccagttcctg caatccttca acctgagcag caaacaggag gcgaaccgcc
    19921 tgctgctgcg gattccggta ttacaaggtt tgctggatgg caaaacagat gtctctattc
    19981 cggcgctgaa actgggcgcg accaaccagc tgcgcttcga ctttgagtat atgaacccga
    20041 tgccgggcgg ttcggtggat aactgtatta ccttccagcc ggtgcagaat catgtggtga
    20101 ttggtgacga ctccaccatc gacttctcga agtattacca cttcatcccg atgccggatc
    20161 tacgcgcctt tgctaacgcg ggcttcccat tcagccggat ggcggatctg tcgcaaacca
    20221 tcaccgtgat gccgaaagcg cctaacgaag cacagatgga aacgttgctg aatactgttg
    20281 gttttatcgg cgcacagacg ggcttcccgg cgattaatct gacggtgacc gatgatggca
    20341 gcaccattca gggcaaagat gccgacatca tgatcatcgg tggtatcccg gacaaactga
    20401 aagacgataa gcagatcgac ctattggtgc aggcgaccga aagctgggtg aaaacaccga
    20461 tgcgccagac cccgttcccc agcattgtgc cggacgagag cgatcgcgcg gcagaaaccc
    20521 ggtcaacgct gacctcttcc ggtgcgatgg cgacggtgat tggcttccag tcgccgtata
    20581 acgaccagcg cagcgtgatt gcgctgttgg cagatagccc acgcggttat gaaatgctta
    20641 acgatgcggt gaacgatagc ggcaaacgcg ccaccatgtt cggttcggtc gcggtgatcc
    20701 gcgagtccgg tatcaacagc ctacgtgttg gcgacgttta ttacgtaggt catctgccgt
    20761 ggttcgagcg cgtgtggtat gcgctggcaa accatccgat tctgctggcg gtgctggcgg
    20821 ctatcagtgt gatattgctg gcatgggtac tgtggcgtct gctgcgaatt attagtcgtc
    20881 gtcgtcttaa cccggataac gagtaattga agatgaatgt gttgcgtagt ggaatcgtga
    20941 cgatgctgtt gttggctgcc tttagtgttc aggcagcctg tacctggcct gcctgggagc
    21001 agtttaaaaa ggattacatc agtcaggaag ggcgcgtcat cgaccccagc gacgcgcgca
    21061 aaatcaccac ctccgaaggg caaagttacg gtatgttctt tgccctggcg gctaacgacc
    21121 gtgcagcttt cgataatatt ctcgactgga cgcagaacaa tctcgctcag ggttctttaa
    21181 aagaacgttt gcccgcctgg ctgtggggca agaaagagaa cagtaagtgg gaagtgctgg
    21241 acagcaattc ggcctccgat ggtgatgtct ggatggcctg gtcgttgctg gaggcggggc
    21301 gtttgtggaa agagcagcgt tataccgaca tcggcagcgc gttgctaaaa cgtatcgcgc
    21361 gggaggaagt ggtgacggtg cctgggctgg gttccatgtt gttaccgggc aaagtgggtt
    21421 ttgctgagga taacagctgg cgttttaacc ccagctacct gccgccgacg ctggcgcagt
    21481 atttcacccg ctttggcgcg ccgtggacta cgctgcgcga aaccaatcaa cgtttattgc
    21541 tggaaaccgc cccgaaaggc ttttcgccag actgggtgcg ttatgagaaa gacaaaggct
    21601 ggcagctaaa agccgaaaaa acattgatca gcagctacga cgccatccgc gtttacatgt
    21661 gggtaggcat gatgcctgac agcgatccgc agaaagcgcg gatgctcaac cggtttaaac
    21721 cgatggcgac attcactgag aaaaacggtt atccgccgga aaaagtggat gtggctacgg
    21781 ggaaagcgca gggtaaagga ccagtcggtt tttctgccgc catgctgccc tttttacaaa
    21841 accgcgatgc gcaggccgtt cagcgtcagc gcgtggccga taactttccc ggcagcgatg
    21901 cctattacaa ctatgtgctg accctgtttg gacaaggctg ggatcaacac cgtttccgct
    21961 tctcgacaaa aggtgagtta ttacctgact ggggccagga atgcgcaaat tcacactaaa
    22021 catattcacg ctttccctcg gtctggccgt catgccgatg gtcgaggcag caccaaccgc
    22081 tcagcaacag ttgctggagc aagttcggtt aggcgaagcg acccatcgtg aagatctggt
    22141 gcaacagtcg ttatatcggc tggaacttat tgatccgaat aacccggacg tcgttgccgc
    22201 ccgtttccgt tctttgttac gttagggcga tattgatggc gcgcaaaaac agctcgatcg
    22261 gctgtcgcag ttagcgccga gttcaaatgc gtataaatcg tcgcggacta cgatgctact
    22321 ttccacgccg gatggtcgtc aggcactgca acaggcacga ttgcaggcga cgaccggtca
    22381 tgcagaagaa gctgtggcga gttacaacaa actgttcaac ggtgcgccgc cggaaggtga
    22441 cattgctgtc gagtactgga gtacggtggc gaaaattccg gctcgccgtg gcgaagcgat
    22501 taatcagtta aaacgcatca atgcggatgc accgggcaat acgggcctgc aaaacaatct
    22561 ggcgctattg ctgtttagta gcgatcgccg tgacgaaggt tttgccgtcc tggaacagat
    22621 ggcaaaatcg aacgccgggc gcgaaggggc ctctaaaatc tggtacggga agattaaaga
    22681 catgcccgtc agtgatgcca gtgtgtcggc gctgaaaaaa tatctctcga tctttagtga
    22741 tggcgatagc gtggcggctg cgcaatcgca actggcagaa cagcaaaaac agctggccga
    22801 tcctgctttc cgcgctcgtg cgcaaggttt agcggcggtg gactctggta tggcgggtaa
    22861 agccattccc gaactacaac aggcggtgcg ggcgaacccg aaagacagtg aagctctggg
    22921 ggcgctgggc caggcgtatt ctcagaaagg cgatcgcgcc aatgcagtgg cgaatctgga
    22981 aaaagccctc gcactggacc cgcacagcag caacaacgac aaatggaaca gtctgctgaa
    23041 agtaaaccgc tactggctgg cgatccagca gggcgatgct gcgctgaaag ccaataatcc
    23101 tgaccgggca gaacgcctgt tccagcaggc gcgtaatgtc gataacaccg acagttatgc
    23161 agtgctgggg ctgggcgatg tggcgatggc gcgaaaagat tatcccgccg ccgaacgtta
    23221 ttatcagcag accttgcgta tggacagcgg caacactaac gccgtgcgcg ggctggcaaa
    23281 tatttaccgc cagcaatcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
    23341 tcagcggcgt agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
    23401 ggcagaggca ctggaaaacc agggcaaatg ggcgccggcg gcagcacttc agcggcaacg
    23461 actggcgctg gaccccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
    23521 ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagtcgaa
    23581 cgacccggag caggtttacg cttacgggct gtacctctct ggtcatgacc aggacagagc
    23641 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct
    23701 ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg
    23761 caaagaagct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc gtattgacct
    23821 cacgctggct gactgggcgc agcagcgacg tgattacacc gccgcccgcg ctgcatatca
    23881 gaatgtcctg acgctggagc caactaacgc cgatgccatt ctggggctga cggaagtgga
    23941 tattgctgcc ggtgacacag cggcggcacg tagccagctg gcgaaactgc ccgctaccga
    24001 taacgcctcg ctgaacacac agcggcgcgt ggcgctggcg caggcgcagc ttggagatac
    24061 cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
    24121 gatggaaagc gcgatggtgc tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc
    24181 aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga ctacgacgcg
    24241 tccgcaggat aacgacacat tcacccgact gacccgtaac gacgagaaag atgactggct
    24301 gaaacgcggc gtgcgcagcg atgcggcggg cctctatcgc cagcaggatc ttaacgtcac
    24361 cctcgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
    24421 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
    24481 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
    24541 ctggggcacc tgtacattac aggactgtag tggcaaccgc agccagtcgg actccggtgc
    24601 cagcgtggag gtcggctggc gaaatgacgt ctggagctgg gatatcggta ccacgccgat
    24661 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
    24721 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
    24781 gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt
    24841 ggggctaagt ctgagctacg ataaaggtga agcaaacggc gtctgggcat cgcttagtgg
    24901 cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
    24961 ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
    25021 ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt actacagtcc
    25081 gcaggaatac ctgtcgtttg ctataccggt gatgtggcgg gagcgcacgg aaaactggtc
    25141 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
    25201 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
    25261 tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac
    25321 ttccaactgg tttgttggca cggcaattga tatccagcag gcgaaagatt acgcacccag
    25381 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    25441 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    25501 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    25561 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    25621 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    25681 ggctacgcaa ctggaaagca ttgctcgctc tgtacgcgaa cccttatctt cagctatttt
    25741 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    25801 cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc
    25861 agagcgcccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct
    25921 gggcgtttac tcgctcgaac gtccggcaaa cccgcagcca attgcctatc tggtactaca
    25981 ggcggattcc ttccgtatgt ataagttcgt gatgagcacc ctctcaacgt tagtgaccat
    26041 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat
    26101 tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    26161 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    26221 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    26281 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    26341 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    26401 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    26461 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc
    26521 tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc accttaggtc agcaagtgct
    26581 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctctgtccgc actgtagcat
    26641 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    26701 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    26761 gatggaagcc gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    26821 tcatcagttt gctatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    26881 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt
    26941 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    27001 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    27061 cctctctgcg ctgcaactga tgcacccaaa tatggtggcg gatatgctgg aactgttaac
    27121 ccgctatcgc attcaaccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    27181 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    27241 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    27301 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    27361 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    27421 cgtggagact gaagcacaac gcgactggct ggcaaaagcg ggcgttggta ttgcccaggg
    27481 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    27541 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    27601 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    27661 tgttatttta aggccgcagg tacctcataa ccttacaaga cctgtagttt tactaaagga
    27721 caccctatga aaacctctct gtttaaaagc ctttactttc aggtcctgac agcgatagcc
    27781 attggtattc tccttggcca tttctatcct gaaataggcg agcaaatgaa accgcttggc
    27841 gacggcttcg ttaagctcat taagatgatc atcgctcctg tcatcttttg taccgtcgta
    27901 acgggcattg cgggcatgga aagcatgaag gcggtcggtc gtaccggcgc agtcgcactg
    27961 ctttactttg aaattgtcag taccatcgcg ctgattattg gtcttatcat cgttaacgtc
    28021 gtgcagcctg gtgccggaat gaacgtcgat ccggcaacgc ttgatgcgaa agcggtagcg
    28081 gtttacgccg atcaggcgaa agaccagggc attgtcgcct tcattatgga tgtcatcccg
    28141 gcgagcgtca ttggcgcatt tgccagcggt aacattctgc aggtgctgct gtttgccgta
    28201 ctgtttggtt ttgcgctcca ccgtctgggc agcaaaggcc aactgatttt taacgtgatc
    28261 gaaagtttct cgcaggtcat cttcggcatc atcaatatga tcatgcgtct ggcacctatt
    28321 ggtgcgttcg gggcaatggc gtttaccatc ggtaaatacg gcgtcggcac actggtgcaa
    28381 ctggggcagc tgattatctg tttctatatt acctgtatcc tgtttgtggt gctggtattg
    28441 ggttcaatcg ctaaagcgac tggtttcagt atcttcaaat ttatccgcta catccgtgaa
    28501 gaactgctga ttgtactggg gacttcatct tccgagtcgg cgctgccgcg tatgctcgac
    28561 aagatggaga aactcggctg ccgcaaatcg gtggtggggc tggtcatccc gacaggctac
    28621 tcgtttaacc ttgatggcac atcgatatac ctgacaatgg cggcggtgtt tatcgcccag
    28681 gccactaaca gtcagatgga tatcgtccac caaatcacgc tgttaatcgt gttgctgctt
    28741 tcttctaaag gggcggcagg ggtaacgggt agtggcttta tcgtgctggc ggcgacgctc
    28801 tctgcggtgg gccatttgcc ggtagcgggt ctggcgctga tcctcggtat cgaccgcttt
    28861 atgtcagaag ctcgtgcgct gactaacctg gtcggtaacg gcgtagcgac cattgtcgtt
    28921 gctaagtggg tgaaagaact ggaccacaaa aaactggacg atgtgctgaa taatcgtgcg
    28981 ccggatggca aaacgcacga attatcctct taatctcacc acttatgccc gcactccctt
    29041 ccccgagtgc gggtgtttgc gcataattga cccctgtgcc tgaacttacc acctgggtga
    29101 gttcaggttt atttcacttc ttccgtgaca ttttcatgtt cttgcggtct aacacgaagt
    29161 gtttttaacg tcatttagac tgactgacaa cgggcagttt tttattacca ggatagttga
    29221 tcaggggttc acatgcaggg cacaaaaatt cgacttttag cgggcggttt gctgatgatg
    29281 gccactgctg gctatgtgca ggcagatgcg ctccagcctg atccagcatg gcaacagggg
    29341 acgctttcca acggtttaca gtggcaagtg ctgaccaccc cccagcgtcc cagcgatcgt
    29401 gttgaaattc gcctgctggt taataccggt tcgctcgccg aaagtacaca acagagcggt
    29461 tacagtcacg ccatccctcg tattgcgcta acgcaaagcg gtggccttga cgcagcacag
    29521 gcgcgttcat tgtggcagca ggggatcgac cctaaacgcc cgatgccgcc ggtaattgtc
    29581 tcttatgaca ccacgctgtt taatctgagt ttgcccaata accgtaacga tttgctgaaa
    29641 gaagcgctct cttatctggc aaatgccact ggcaaattga ccatcacacc agaaaccatc
    29701 aaccacgcgc tgcaaagtca ggacatggtg gcaacctggc ctgccgatac taaagagggc
    29761 tggtggcgct atcgtttgaa agggtcaacc ttgttaggtc acgatcctgc cgatccgctg
    29821 aaacaacccg ttgaagcgga aaaaattaaa gatttctatc agaaatggta caccccggat
    29881 gcaatgacgc tactggtggt gggaaacgtg gatgcgcgct cggttgtcga ccaaatcaac
    29941 aaaacgtttg gcgaactgaa aggcaaacgt gaaacgccag ctccggtgcc gacgctttct
    30001 ccgctgcgtg cggaagcggt gagtattatg actgacgcgg tgcgtcagga ccggttatct
    30061 atcatgtggg atacgccgtg gcagccgatt cgtgaatcag ccgcactgct gcgctactgg
    30121 cgtgcggacc tggcccgcga ggcgctgttc tggcatgttc agcaagcgtt aagcgccagt
    30181 aacagcaaag acatcggtct tggatttgac tgccgtgtgc tgtatctgcg tgcgcagtgt
    30241 gccatcaaca tcgaatcacc aaacgacaag ctgaacagca accttaatct ggtggcgcgt
    30301 gaactggcga aggttcgcga taaaggtctg ccggaagaag agttcaatgc gttagtggcg
    30361 caaaagaaac tggagctgca gaaactgttt gccgcctatg cgcgggctga taccgatatt
    30421 ctgatgggtc agcggatgcg ttcgttgcaa aatcaggtcg tcgatatcgc gccggagcag
    30481 tatcagaaac tgcggcagga tttccttaac agcctgacgg tggagatgtt aaatcaggat
    30541 ctgcgtcagc agttgtcgaa tgatatggcg ttaatactgc tgcagccgaa aggcgagccg
    30601 gaatttaaca tgaaagcgtt gcaggcggcc tgggatcaaa tcatggcccc atcgactgct
    30661 gctgccgcca cctctgtcgc cacggatgac gtacatcctg aagtgacgga tattccacct
    30721 gcacagtaat gcctgatggt gatgagttag cacaaccatc aggtatttat ggatgagctg
    30781 gatagagggg taacacttta tccagccttt tgcatatgct gcgtttacgc tggcatcgcc
    30841 tcacgcggga taatcgcgcc gcgatactga ataacggtac ttgcggtcag gtgcccgcgt
    30901 ttcgccgcgt tttccgcact gccgcccgtc aaacgtaccg ccagataacc ggcgctgaaa
    30961 gagtcgccag ctgcggtggt atcgatcact ttttctttcg gcagtttcac cgccggaaca
    31021 tccactaacc cttcgccagc aatggacacc aggcaagaat ccgccccgcg tttcaccacc
    31081 acttctttca cgcccgcgtt atgggtgcgc gcaatgacgt cttccaccgg ctgttgaccc
    31141 cacagcgcgt cttcatcgtc cagcgtcagg aaggcgatat ccgtgcattc cagcatttgt
    31201 tggtacacct gctgtgtctc ttctttgctg gcccacaggc gcggacgata gttattgtcg
    31261 aaaatcactt ttccgccgtt ggcgcggcat tcgcgcagca gggaaagcag cttttcgcgg
    31321 ctggtcgggc ttaagatcgc caggctaatc ccgctcaggt agagataatc gaaattcgcc
    31381 agctcttcgc aaatcgccgt agactgctca ctctccagcc agaatttggc ggcggcttcg
    31441 ttccgccagt agtagaacgt acgctcgccg gtgctgtcgg tttcaatgta gtaaaggccc
    31501 ggcagacggt tttccatccg ttgggtcagg gaaatatcaa cgttctcgcc gtgccaggcg
    31561 tccagcatct gctggctaaa actgtccgtt cccagcgccg ttacgtaatg aacggttaat
    31621 gccgcaggat cgacctgacg ggcgatatag acggaagtgt tcagggtatc gccgccgaaa
    31681 ccgcgcttaa cgtccgcgcc tttctcggaa agctcaatca tgcattcgcc aatcacggca
    31741 atctttttgg acatagtcgt gaacctgatc tgtaaaaaat tagcgttagt ttgcgctgtg
    31801 gtggtttgct ggtcaacaat attaaaacag tgttccatta tttttttgag ccagaacgta
    31861 ttcctgaaag attccgttgt ggagtgagga aatttacccc ggccaggcgg taaagttctg
    31921 cccttacgcg ccgataatct ttgtcgagtc cgggcagcat cacttttaaa cacaggacat
    31981 ctttgatgat aaggcaggtt atccagcgaa taagcaaccc tgaagcaagc atcgagagct
    32041 tgcaggaacg gcgtttttgg ttgcagtgtg agcgtgctta cacctggcag ccgatctatc
    32101 aaacatgcgg gcggttaatg gccgtggagc tattaacggt ggtcacgcat cccttgaacc
    32161 cttcgcaacg cctgccgccg gatcgctatt ttactgaaat caccgtcagc catcggatgg
    32221 aggttgtgaa agagcagatt gatttgctgg cgcaaaaagc cgacttcttt atagagcacg
    32281 gcctgctggc atcggtcaat attgatggcc ctacgctcat cgccctgcgt cagcaaccaa
    32341 aaatcctgcg ccaaattgag cgtcttccct ggctgcgttt cgaactggtg gagcatatcc
    32401 gtctgccgaa agattcaacc tttgcctcga tgtgtgaatt tggcccgctg tggctgggtg
    32461 attttggtac cgggatggca aatttctctg cactaagtga agtgcgttat gactacatca
    32521 aaatcgcgcg agaactgttt gtgatgctgc gtcagtcgcc ggaaggacgc acactctttt
    32581 ctcagctttt acatctaatg aatcgctatt gtcgcggggt gattgttgag ggcgtagaaa
    32641 cgccggaaga gtggcgtgat gtacagaact cgcccgcatt cgccgcacaa ggctggtttc
    32701 tttcacgccc ggcaccgata gaaacgctga atacggcggt tctggcgcta taagctgcct
    32761 cattttccgc ctggctggac tatctttagg actggcacag gaagaatatg aggaagcgaa
    32821 cgatgagcaa ggcaggcaaa ataactgctg cgatttcagg ggctttcttg ttgttgattg
    32881 tcgtggcgat cattttgatt gcaacttttg actggaatcg actcaaaccg accatcaacc
    32941 agaaagtctc tgcggagttg aatcgtccgt tcgctatccg tggcgatctg ggcgtggtgt
    33001 gggagcggca aaaacaagaa actggctggc gcagctgggt gccgtggcct catgtacatg
    33061 cagaagacat cattcttggc aatccaccgg atattcccga agtcacgatg gtgcatttgc
    33121 cgcgagtaga ggcaacgctg gcaccgctgg cgctgctgac caaaacggtc tggctgccgt
    33181 ggatcaagct cgaaaagccc gacgcgcgcc tgattcgcct ctccgaaaag aacaataact
    33241 ggacgtttaa tctcgccaac gatgataaca aagacgcgaa tgcaaagccg tcggcatggt
    33301 cgtttcggct ggataatatt cttttcgatc aagggcggat cgccattgat gacaaagtaa
    33361 gcaaagcgga tctggaaatt tttgttgatc cgttaggcaa gccgctgccg ttcagcgaag
    33421 ttactggatc gaaaggtaaa gcggataaag aaaaggtggg cgattacgtt tttggcctga
    33481 aggcgcaggg acgttataac ggtgaaccgc tcacgggtac gggaaaaata ggcggtatgc
    33541 tggcgctgcg tggcgaaggg acgccgtttc cggtacaggc tgatttccgt tcaggtaata
    33601 cccgtgttgc ttttgatggc gtcgtgaatg acccaatgaa gatgggcggt gtcgatttac
    33661 ggcttaaatt ttctggcgat tcactgggtg atctctatga actgacgggc gttctgctgc
    33721 ccgatacccc gccgtttgaa acggatggtc ggctggtagc gaaaatcgac actgaaaaat
    33781 cgtcggtctt tgattatcgc ggttttaatg ggcgaattgg cgatagcgat atccacggtt
    33841 ctctggtcta caccaccgga aagccgcgac caaaactgga aggtgatgtc gagtcgcggc
    33901 aattgcggct ggcggacctg ggaccgttga ttggcgttga ttcggggaaa ggggcagaaa
    33961 agtcgaaacg gtctgaacag aagaagggcg aaaaaagcgt tcagcctgcg ggcaaagtgc
    34021 tgccttatga ccgcttcgaa accgataaat gggacgttat ggatgccgat gttcgcttca
    34081 aaggccgacg tatcgagcat ggcagtagcc tgccgattag cgatctttct actcatatca
    34141 tcctcaaaaa tgctgacctg cgcctgcaac cgctgaaatt tggcatggcg ggcggcagca
    34201 ttgcggcgaa tatccatctg gaaggcgata aaaagccgat gcaggggcgg gcagatattc
    34261 aggctcgtcg actgaaactg aaagaactga tgcccgatgt ggaactgatg cagaagacgc
    34321 tgggggaaat gaacggtgac gcggaactac gcggtagcgg taactcggtg gcggcgcttt
    34381 taggcaacag taacggcaac ctgaaactgt tgatgaatga cgggctggtg agccgcaacc
    34441 tgatggagat tgttgggctg aatgtcggca actacattgt cggtgcgata tttggtgacg
    34501 atgaggtgcg ggtgaactgc gcggcggcga atctgaatat tgccaacggc gtagcgcgcc
    34561 cgcagatttt tgctttcgat actgagaacg cgttgattaa tgttaccggc acggcaagtt
    34621 ttgcttcgga acagctggat ttgactattg atccggagag taaagggatt cggattatca
    34681 cactgcgttc gccgctgtat gtgcgtggga cgtttaaaaa tccgcaggct ggggtgaaag
    34741 ccggaccgct gattgcccgt ggtgccgttg cggcggcact ggcaacgctg gtaacaccgg
    34801 cggcggcgtt attggcactg atctcacctt ccgaagggga ggctaatcag tgtcggacga
    34861 tattgtcgca gatgaagaag tgatgaggtt ggattgtgtt tgctggtgag ttcgggagca
    34921 gcgtgttccc ctcaccctaa tcctctcccc aaaggggcga ggagactgtt ttgtgcgtgt
    34981 tgaaacaacg agtaccgctt ttccgttcac tttctggggg agggctgtta tgttcgcgat
    35041 ataacatctc gctccgcttt tctccctctc cctttcaggg agagggctgg ggtgagggtg
    35101 gctgcccaca cgatacttac aacgactgat gttgcgtctc atgggtcagc agcagggcga
    35161 ttaacgtcaa gccagccatc gccgccagat ataaccccac cgcacctaac ccgtagttag
    35221 tctgcaacca ggctgcgata tacggcgcaa cggaagcccc gagaatcgac gatacgttgt
    35281 aagagaacga tgctccggtg taacgcactt ctgtcgggaa cagctctggt aacagcgcgc
    35341 ccatcggacc gaaggtcagc cccatcagac ttaaccccag cagcaggaag gcaaaaacca
    35401 gaatcgggtt gccagaaccg agcaaagggt taaaggcgaa tagcgcgaac aggatgagca
    35461 gcgtggtgat gattaccatg cttttacggc gaccaaaggc atcagccagt aatccagcga
    35521 ctggcaccat cacgccaaaa ccaataactg ccatcatcaa catccacaac acttcgttac
    35581 gcggcaggcc aagcccaact ggcgcggcgg cggtactaaa ggtcatggag tagaccgtca
    35641 tgatgtaaaa cagcgtatag gttgccagca taatgaacgt acccagtacg gttacgcgaa
    35701 catgtttggt cagcagcgta cccagcggga tcttcacctg ttttttcgct ttagcgactt
    35761 tctcaaacac cggcgactca tgcagcgaca cgcgaacata caggccgata atgaccagca
    35821 ccgccgagaa gataaatggc acacgccagc cccagctcat aaactgctcg tcggtcagta
    35881 gccaggaaag cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt gcgcccagct
    35941 gcgggaagga gccgtacaat gcacgtttgc gcggtggggc gttttcagtc gccagcagcg
    36001 ccgcgccgcc ccattcacca cctaagccca gaccctgacc aaatcgagcc agcgccagca
    36061 gcagcggggc gaaaatacca atcgtggcat agcctggcag cagaccaatc accacggtcg
    36121 aaatccccat cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg cgatcgccaa
    36181 aatgaccaaa aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg aaggtggcga
    36241 gcgactgtag cgttgctgcc gtaggatcgc cctgcgggaa gaagatatgc ggaaacacaa
    36301 taacggccgc agtggcgtaa atgtaaaagt cgaagaactc aatggctgtg ccaatgagag
    36361 aggcgacaag gactttatta cgcgagttga tcggcgtgta ttcttgctcg tggtcgagtg
    36421 ttgtggctgt tgcttgcata atcttttctt attttttggc taacgaatag ccatattacg
    36481 cacagcaaaa gcgatatttc aatgtgtaac aaatcgcgga aatggtcaaa aaccaggcaa
    36541 aaaggggata atgtttaggc cagtgatttt tcttctatga aatgcttcac ataattttat
    36601 aaatagtgga taaaactggt ttcaggttaa ataaaagtta ccaactggat ttatatgctg
    36661 gggttatgag ttagaatgaa attgtttgct cccaaataga tggctgaaga ggctctgggg
    36721 acgagtttca agaggttatg tgcgcattat agccggatgc gatgctggcg catcttatcc
    36781 ggcctacggg gagccaggcc gaataaaatc gtatgccgga taaggcgttt acgccgcatc
    36841 cggcctattt taaggctgcg ttttccctgg cattcgcggg tcgtctttat attcggcggt
    36901 ggcaatccac gccgcgcaaa acagcgtcaa acgggcgaag aagtagaaaa atgccatcag
    36961 ccccagcacg gaaccaaatg ctgcgccaga gggggatttc atcagcgatg gcagggtgta
    37021 ggtcatcacg attttaatca cttcaaaacc aatagcagcc agaaatgttc cgcgaatcag
    37081 ggcttttttg cgcggacggt ggcgcggcaa ccgccagaag atccagaaga aaagcagata
    37141 gttggcgaag atggaaatcg ccaggccaat caatcgccac gtcggtttca gccactcaat
    37201 gctgttcagg tgcagggcgc taataatcat ttgctgcgcc gaaccggcaa ccgaggtgat
    37261 cgaaagcgtc acaatcagcg caatcaacaa accaatcagt gaaataaaat cacgcagata
    37321 ttttacccag aacttctcct gatcttgcgg cgagcgttcc cagacatcac gcgactgggc
    37381 acgaatcgct tcacgcaggt tacccatcca gttgatgccg gaataaagtg ccaccgccag
    37441 gccgacaagc cctacagtcg tacgctgctg aacggcggtg ttgatggtgt ttttcaacgt
    37501 ggcggctagt gtcggatcgc tgatgttttg cagaattttg tcgaagatat cctgtagcaa
    37561 catcggatgg gaggccagca caaagccccc tgcggcaaac gacaccatca aaatcgggat
    37621 catcgacaaa aatgagaaat aggtgatagc cgcaccaaac tggttaccca gccgatcgtt
    37681 aaagcgttct gtcgcgcgaa tcaggtgcgc aataaccggt tggcgttgga ctttttcggc
    37741 agtggtggtg acggtttcca gcgcctggct aactttgccg cctttctcgc tattatccaa
    37801 ctgcttaatc ggctcgtgct ccagatcctg ggtgggacgt ttgatctcgt tttcctgcgt
    37861 catccgctct tttgtccttc tgtcgtggtc attcttaaaa agtatagtcg gtcagtccag
    37921 gtgctctttc attacgccag ccagccactg cataaacagg tttacacgcc gggaaagctc
    37981 ccgacgctgc gggtaaacca gcgaaaggga gagcggctcg gcacggtagc caggtaatac
    38041 ttcaataagc cgcccggcac gcagggcttc gcgcacggca atgcgcggga tctgaataat
    38101 ccccagaccg gcaagaccgg cggcgagata gttttcgcta ctatttaccg tcaacatgcc
    38161 gccagactta aaccactgga cgccattaac gctggcaacc tcaaaaccta acggatgtac
    38221 acccaggtgc ggtgtataac gcactattgc gtgtgaagtc agatcatcgg ggctttgcgg
    38281 ataaccaaag cgcgtcagat agtgcggact ggcacagttg accatggtca gtttgccgag
    38341 gggacgggcg ataacgccat cttccggtaa cgcgccagtg cgtatcacgc aatcaaaacc
    38401 atcatgaaga atatctaccg gacggtcatg gctactcagt tccagctcaa ttcccggatg
    38461 cagatagaga aattccgaca ggcgcggcag taacaggttt ttcgcgattc ctggcgggat
    38521 gtcgatgcgt aatttaccgc tgatactggt ggcatcctgt tgaaacagac cgtccagttc
    38581 gttgagatta ctcaacacat cttttgcccg ttgataatag gtcatgcctt ctggcgtgag
    38641 tttgacccgt cgcgtggtgc gctggagaag ctgggtgcca agttgatgtt ccagtgcctg
    38701 tatctggcgc gaaacacttc cctttggcaa agcaaagaaa tccgctgcgc gggaaaaact
    38761 ttccagctcc gcgactttga tgaacaactg cattgcgtga attttatcca tttctttgac
    38821 cgattgttgt tcacagcgaa acagtaacgc gcatatcgca ttgtttattg ttttttctaa
    38881 catctaatgc cgggtttcac aatgttgtca tgacgaatgt ggcgatttct cagcaggaaa
    38941 agggcggaat agctttatga gaaaggtgaa tccccttaaa cagagcgtat ttttcagtga
    39001 ttccctttaa agtgaataga ggacgtattg gctatttgtg cttatttttg tctcattgcc
    39061 atttgttatt ttggcttgta gtcgcggaat ttcattttta attgcatgcg tactaaccac
    39121 caatataagg aaatcctcaa aattgtttta aatagtgccc tcgacatgta atttcaggcg
    39181 tttagctgta actatttata tcggtaacta ttttctacgc gtcatctctg tagaatggcc
    39241 accgccaaat ggcgaattct tattttttct cgcgggtaat acccttccac tacttaagtc
    39301 tttttaacat attcaggtgg agattcagtt atgcaaatag tcatgtttga caggcagtca
    39361 atatttattc atggaatgaa aatcagttta cagcagcgta ttccaggggt gagtattcag
    39421 ggggccagtc aggcagacga gttatggcaa aagctggaaa gttaccctga agccttagtt
    39481 atgctcgatg gcgatcagga tggtgagttt tgctactggt tgctgcaaaa aaccgtggtg
    39541 cagtttcctg aggttaaggt gttaatcacg gcgacggatt gcaataaacg gtggttacag
    39601 gaagttatcc attttaatgt gctggccatt gtgcctcgtg attcaaccgt cgagacattt
    39661 gcgttggcgg taaatagtgc ggcgatgggg atgatgtttc tgcctggaga ctggcggacg
    39721 acgccggaaa aggacattaa agatctcaaa tcgttaagcg cccgccagcg ggagatttta
    39781 accatgttag cggcgggcga gtcaaataag gagatcggca gagcgctgaa tatcagtacc
    39841 ggaacggtaa aagcgcatct ggaatccctg tatcgccgtc tggaagtgaa aaatcgcacc
    39901 caggcggcaa tgatgttaaa tatctcctcc tgaagaggat aattttacag ccagcaggaa
    39961 gttagcggct ggctgtaaaa atattatggt tcgccgtaca aaccaattaa acggcgtacc
    40021 acaccgttag tccagccaaa cccatcctgc aacggatact cgccaccgcc gccttcgcgt
    40081 ggaacaccat cggcaatatg gtatttttcg atcagtttgt gctgttccag atagaactga
    40141 ttcaccgtct tcagccagct tcgcgcgatt tcatcaccca gaaggtcatc gccgtacatt
    40201 ttaaatccct gaatcgccat ccattgtaac ggtgcccagc cgttgggttt atcccactgt
    40261 tcaccggttt cgtactcgct tgccagaatc ccgccaggtg tcagtaaccg actgcgcacg
    40321 gcatttgcca gacgatcggc ctgttcatgg ttcgccatac cgacatagag tggcacaatg
    40381 gcggcagcgg aaaacagcgc cagttgttcg cgtcgccagt cgtaatcgcg gtagatgccg
    40441 ttttcatcat cccagaggta acgctttacc gcatcgcgac gggcactggc cttctggcgg
    40501 aacagtgctt ctgtctcttt ctcgcctttc agcgccgaga tgttggcgat ggcgctctcc
    40561 agtttaaaca ggaaggcatt cagatcgatg gggatgaact gggtggtacg aatactcgcc
    40621 agacgaccag tatcacgcag ccaacgggaa gagtaatccc aaccggaggc cgcccccgct
    40681 cgtaaatcgc ggtacacctc gttgggcggg cgaccagaat gtttcgcggt ttcaacgtcc
    40741 tcaagccagg attcgtcacg cggcgtgtcg cgatcgtccc agtaacggtt gagcagcgat
    40801 ccgtccggca tccgcacaac atggcgatag gcctgattag ggatcaacga ttctgcaccg
    40861 tccatccaga aggcatattc cattttcagg tggtcgagat agcggcgcgc accgcgtaca
    40921 ccatcttctt caaacaactc caccatcagc gcaaaaaccg gtggttgcga gcggctcaaa
    40981 taataggtgc ggttgccgtt ggggatgtga ccgtagtttt cgatcatcca ggcgaagtta
    41041 tcggccatgc atttcagcaa atcttcccga ccactttccg ccagccccag catggtgaaa
    41101 taggaatccc aatagtaggt ttcgctaaaa cggccgcccg ggacaatata tgactgcggt
    41161 agcgccagca gagaagacca cggaatgtga tcctgtggtt cgcgggttag caccggccac
    41221 agctggtcga tatgctcttt cagggaattt tgcgggtccg atacatactc gctggagtag
    41281 acctccggca gccagaagtg attttcaaca aacttgcgca agtcaaaatc acgatgacgg
    41341 cgcactttac ggtagcggat taaaatatcc agcgggtcca ttttgggtgc gcagtcggga
    41401 aaggttttac tgtcggggaa aattttcgcc gactgaacat gctcgaacag ttcgagatag
    41461 cgatcggcag gcgtcagcgc atcagaggcg ggcagccctt caatcatctc gggttccggt
    41521 tctgcctcga tcatctcatc cagttttaat tcatacgggt ccagttcata gcagagatcg
    41581 acttcgatca tcagttcgtc tggattaggg ttttgaattt tctgattgag cataaggatc
    41641 caacctccga aattcgtcgc taattatcgg cttttcttta agggtagtca ttcgctccgc
    41701 ctggtgggcg acaaagcgtg cggtggatca cgtttattaa cttgtcaaaa aatataacgc
    41761 actaatatca atggaattta taaaaatacg gatttggtgt tgtttttcat tttcagaaca
    41821 ttcaatcctg ctctcgccgc aatcatgata tgccattcct gatctgggta ataggcacag
    41881 gctatcttat tgatagttta tattcatgta attgattgat tcctatcaaa aagcgtatct
    41941 ccctgttttt tatgatggct ttgtctcgca gagacgtttg cagcattctg gaaaattttc
    42001 tgggtcacaa taactcacca ccgtgaggga agaataatga aaatggtctc acgtattacc
    42061 gcgatcggcc tggctggcgt cgcgatttgc tatttagggt tatctggtta tgtgtggtac
    42121 cacgataata aacgcagtaa acaggccgat gttcaggcat ctgctgtcag tgaaaataat
    42181 aaggttttag gctttctccg cgaaaaagga tgcgactatt gccacacgcc ttcggcagaa
    42241 ttacccgcct attattatat tcctggcgcg aaacagttga tggattacga cattaagctt
    42301 ggatataaat cttttaacct cgaggccgtg cgtgcggcac tgctggctga taaacccgtt
    42361 tcgcaaagcg atctgaataa gattgaatgg gtgatgcagt atgaaactat gccaccaacg
    42421 cgttataccg cgctacactg ggcgggtaag gtgagtgatg aagagcgggc ggaaatactg
    42481 gcctggattg caaaacagcg cgcggaatat tacgccagca atgatactgc tccggagcat
    42541 cgcaatgaac cggtgcagcc catcccgcaa aaactgccta ccgatgcgca aaaagtggcg
    42601 ttgggctttg cgctgtatca cgatccccgt ttatcggctg atagcaccat ttcatgcgct
    42661 cattgccatg cgttgaatgc ggggggcgtc gatggcagaa aaacatcgat tggtgttggt
    42721 ggcgcagttg ggccgattaa cgcgccgacg gtatttaact cagtatttaa cgttgagcag
    42781 ttctgggatg gtcgtgcggc aacattgcag gatcaggcag gtggaccgcc gttgaacccg
    42841 attgaaatgg cgtcgaaatc ctgggacgaa attattgcta agctggaaaa agatccgcag
    42901 cttaaagcgc agttcctcga agtttatccg caaggtttca gtggcgaaaa tattactgat
    42961 gccattgctg aatttgagaa aacattaatt acgccggatt ccccatttga taaatggttg
    43021 cgtggagatg aaaatgcgct gacggcgcaa cagaaaaaag gctatcaatt atttaaagat
    43081 aataaatgtg caacttgtca tggtggtatt attctcggcg gacgttcctt tgaaccgttg
    43141 gggctgaaaa aagactttaa ctttggggaa attacggcgg cggatattgg tcgtatgaat
    43201 gtgactaaag aagagcgtga taaattgcgc cagaaagtac ccggtttacg taacgttgct
    43261 ttaacggcac cgtacttcca tcgcggtgac gtgccgacgc tggacggggc ggtgaaactg
    43321 atgctgcgct atcaggtagg caaagagctg ccgcaggagg atgtggatga tatcgtagct
    43381 ttcctgcaca gtctgaacgg ggtgtacacg ccgtatatgc aggataaaca ataactaatt
    43441 tgatcgcccg aacaggaatg tttgggcgat ttttattacg ataataaagt ctgtttttaa
    43501 tattatcatg ttaaatgttt atattataaa aagtcgtttt tctgcttagg attttgttat
    43561 ttaaattaag cctgtaatgc cttgcttcca ttgcggataa atcctacttt tttattgcct
    43621 tcaaataaat ttaaggagtt cgaaatggac cagaagctgt taacggattt ccgctcagaa
    43681 ctactcgatt cacgttttgg cgcaaaggcc atttctacta tcgcggagtc aaaacgattt
    43741 ccgctgcacg aaatgcgcga tgatgtcgca tttcagatta tcaatgatga attatatctt
    43801 gatggcaacg ctcgtcagaa cctggccact ttctgccaga cctgggacga cgaaaacgtc
    43861 cataaattga tggatttgtc gatcaa
//
LOCUS       DADLTD010000005        42380 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLTD010000005 DADLTD010000000
VERSION     DADLTD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.005"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..416)
                     /gene="yeeE"
                     /locus_tag="JN701_000202"
     CDS             complement(<1..416)
                     /gene="yeeE"
                     /locus_tag="JN701_000202"
                     /inference="COORDINATES: protein motif:HMM:NF016064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7362754.1"
                     /translation="MTYEAGAFPWPGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIG
                     SWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITLW
                     VVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVT"
     gene            complement(426..608)
                     /locus_tag="JN701_000203"
     CDS             complement(426..608)
                     /locus_tag="JN701_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HAY7362755.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALI"
     gene            complement(787..2145)
                     /gene="plaP"
                     /locus_tag="JN701_000204"
     CDS             complement(787..2145)
                     /gene="plaP"
                     /locus_tag="JN701_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY7362756.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2135..2197)
                     /gene="yoeI"
                     /locus_tag="JN701_000205"
     CDS             complement(2135..2197)
                     /gene="yoeI"
                     /locus_tag="JN701_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY7362757.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2412..3341)
                     /locus_tag="JN701_000206"
     CDS             complement(2412..3341)
                     /locus_tag="JN701_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7362758.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3387..4211)
                     /locus_tag="JN701_000207"
     CDS             complement(3387..4211)
                     /locus_tag="JN701_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7362759.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4294..4548)
                     /gene="yoeB"
                     /locus_tag="JN701_000208"
                     /pseudo
     CDS             complement(4294..4548)
                     /gene="yoeB"
                     /locus_tag="JN701_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4545..4796)
                     /gene="yefM"
                     /locus_tag="JN701_000209"
     CDS             complement(4545..4796)
                     /gene="yefM"
                     /locus_tag="JN701_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY7362760.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5079..5129
                     /gene="hisL"
                     /locus_tag="JN701_000210"
     CDS             5079..5129
                     /gene="hisL"
                     /locus_tag="JN701_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY7362761.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            5275..6174
                     /gene="hisG"
                     /locus_tag="JN701_000211"
     CDS             5275..6174
                     /gene="hisG"
                     /locus_tag="JN701_000211"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY7362762.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAVCDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6180..7484
                     /gene="hisD"
                     /locus_tag="JN701_000212"
     CDS             6180..7484
                     /gene="hisD"
                     /locus_tag="JN701_000212"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY7362763.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7481..8551
                     /gene="hisC"
                     /locus_tag="JN701_000213"
     CDS             7481..8551
                     /gene="hisC"
                     /locus_tag="JN701_000213"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY7362764.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8551..9618
                     /locus_tag="JN701_000214"
     CDS             8551..9618
                     /locus_tag="JN701_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HAY7362765.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAIDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9618..10208
                     /gene="hisH"
                     /locus_tag="JN701_000215"
     CDS             9618..10208
                     /gene="hisH"
                     /locus_tag="JN701_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY7362766.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10208..10945
                     /gene="hisA"
                     /locus_tag="JN701_000216"
     CDS             10208..10945
                     /gene="hisA"
                     /locus_tag="JN701_000216"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY7362767.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10927..11703
                     /gene="hisF"
                     /locus_tag="JN701_000217"
     CDS             10927..11703
                     /gene="hisF"
                     /locus_tag="JN701_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY7362768.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11697..12308
                     /locus_tag="JN701_000218"
     CDS             11697..12308
                     /locus_tag="JN701_000218"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY7362769.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVAFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12405..13382)
                     /gene="wzzB"
                     /locus_tag="JN701_000219"
     CDS             complement(12405..13382)
                     /gene="wzzB"
                     /locus_tag="JN701_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY7362770.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13529..13621)
                     /locus_tag="JN701_000220"
                     /pseudo
     CDS             complement(13529..13621)
                     /locus_tag="JN701_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13791..14795
                     /locus_tag="JN701_000221"
     CDS             13791..14795
                     /locus_tag="JN701_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HAY7362771.1"
                     /translation="MKFLVTGAAGFIGFYLSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14861..16027)
                     /gene="ugd"
                     /locus_tag="JN701_000222"
     CDS             complement(14861..16027)
                     /gene="ugd"
                     /locus_tag="JN701_000222"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY7362772.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     LVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAKRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKNGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16276..17682)
                     /gene="gndA"
                     /locus_tag="JN701_000223"
     CDS             complement(16276..17682)
                     /gene="gndA"
                     /locus_tag="JN701_000223"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY7362773.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIT
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFHDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDNAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(17787..18620)
                     /locus_tag="JN701_000224"
     CDS             complement(17787..18620)
                     /locus_tag="JN701_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7362774.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
     gene            complement(18617..19486)
                     /locus_tag="JN701_000225"
     CDS             complement(18617..19486)
                     /locus_tag="JN701_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7362775.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKNYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            complement(19503..20690)
                     /gene="wzy"
                     /locus_tag="JN701_000226"
     CDS             complement(19503..20690)
                     /gene="wzy"
                     /locus_tag="JN701_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen polymerase"
                     /protein_id="HAY7362776.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMEGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            complement(20746..21978)
                     /gene="wzx"
                     /locus_tag="JN701_000227"
     CDS             complement(20746..21978)
                     /gene="wzx"
                     /locus_tag="JN701_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen flippase"
                     /protein_id="HAY7362777.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGTEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRAIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANYVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVMLIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDTKY"
     gene            complement(21975..22532)
                     /gene="rfbC"
                     /locus_tag="JN701_000228"
     CDS             complement(21975..22532)
                     /gene="rfbC"
                     /locus_tag="JN701_000228"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY7362778.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            complement(22537..23415)
                     /gene="rfbA"
                     /locus_tag="JN701_000229"
     CDS             complement(22537..23415)
                     /gene="rfbA"
                     /locus_tag="JN701_000229"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7362779.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(23473..24372)
                     /gene="rfbD"
                     /locus_tag="JN701_000230"
     CDS             complement(23473..24372)
                     /gene="rfbD"
                     /locus_tag="JN701_000230"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY7362780.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKEANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAAKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAI"
     gene            complement(24372..25457)
                     /gene="rfbB"
                     /locus_tag="JN701_000231"
     CDS             complement(24372..25457)
                     /gene="rfbB"
                     /locus_tag="JN701_000231"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7362781.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTRFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTLEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            complement(25830..26723)
                     /gene="galF"
                     /locus_tag="JN701_000232"
     CDS             complement(25830..26723)
                     /gene="galF"
                     /locus_tag="JN701_000232"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY7362782.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(26955..27950)
                     /locus_tag="JN701_000233"
     CDS             complement(26955..27950)
                     /locus_tag="JN701_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7362783.1"
                     /translation="MNNNVLLIGASGFVGTRLLETAIADFNIKNLDKRQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(28108..29502)
                     /gene="wcaM"
                     /locus_tag="JN701_000234"
     CDS             complement(28108..29502)
                     /gene="wcaM"
                     /locus_tag="JN701_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY7362784.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMSNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLVYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(29513..30733)
                     /gene="wcaL"
                     /locus_tag="JN701_000235"
     CDS             complement(29513..30733)
                     /gene="wcaL"
                     /locus_tag="JN701_000235"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY7362785.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTHWLQDEPTGKIAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLGTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(30730..32010)
                     /gene="wcaK"
                     /locus_tag="JN701_000236"
     CDS             complement(30730..32010)
                     /gene="wcaK"
                     /locus_tag="JN701_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY7362786.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGCQV
                     IALSTCTGIDSYNKDDRMMALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(32286..33764)
                     /gene="wzxC"
                     /locus_tag="JN701_000237"
     CDS             complement(32286..33764)
                     /gene="wzxC"
                     /locus_tag="JN701_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY7362787.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCLAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPQGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKPLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQFLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(33766..35106)
                     /gene="wcaJ"
                     /locus_tag="JN701_000238"
     CDS             complement(33766..35106)
                     /gene="wcaJ"
                     /locus_tag="JN701_000238"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY7362788.1"
                     /translation="MVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQML
                     GGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
                     IGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYH
                     DPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLL
                     IPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLC
                     CIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKV
                     GNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPCITG
                     WAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY"
     gene            complement(35211..36581)
                     /locus_tag="JN701_000239"
     CDS             complement(35211..36581)
                     /locus_tag="JN701_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAY7362789.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTSVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPANGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(36774..38210)
                     /gene="cpsB"
                     /locus_tag="JN701_000240"
                     /pseudo
     CDS             complement(36774..38210)
                     /gene="cpsB"
                     /locus_tag="JN701_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
     gene            complement(38213..39436)
                     /gene="wcaI"
                     /locus_tag="JN701_000241"
                     /pseudo
     CDS             complement(38213..39436)
                     /gene="wcaI"
                     /locus_tag="JN701_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(39433..39912)
                     /locus_tag="JN701_000242"
     CDS             complement(39433..39912)
                     /locus_tag="JN701_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY7362790.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLPGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(39915..40880)
                     /gene="fcl"
                     /locus_tag="JN701_000243"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(39915..40880)
                     /gene="fcl"
                     /locus_tag="JN701_000243"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY7362791.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQSTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(40883..42004)
                     /gene="gmd"
                     /locus_tag="JN701_000244"
     CDS             complement(40883..42004)
                     /gene="gmd"
                     /locus_tag="JN701_000244"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY7362792.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYCESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIVTGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLDDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(42030..>42380)
                     /gene="wcaF"
                     /locus_tag="JN701_000245"
     CDS             complement(42030..>42380)
                     /gene="wcaF"
                     /locus_tag="JN701_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY7362793.1"
                     /translation="TYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQKSYLCTGS
                     HDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCR
                     GNPAVVIRERVETE"
BASE COUNT        11094 a        10764 c         9785 g        10737 t
ORIGIN      
        1 gtgacaaagg gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc
       61 ggtaaggtcg cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt
      121 atcaccagca aaacggcaac caacggccac acagacaaat taaaagtctc agcaatagag
      181 ttatgttcag tactgtagtg ctgcaaggtt tgatttaaac cactggcatg tggagaacgc
      241 atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca
      301 cccgcgcgat accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaag
      361 atatacccac ctataacagt accaggccac gggaacgccc cggcttcgta agtcagtagg
      421 cccgcctaaa ttaacgcaaa aacccccaca ctttgtaccg aaattgcaat cagcaaggcg
      481 taaaacatgc gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa
      541 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
      601 gaaaacataa attaattaac cagatgaatg ttaatgagga aattattcat gactggtgga
      661 atgcagacca ataaccaaat tctctaaatt agaacaaatg gttcttaatg aggatttcga
      721 taaaaaagaa taaaaaaacc ggagccaaat attcattcga ctccggttga ttattagaaa
      781 agatgattat gctacgtctt cgtactgcgg aaccgggtta cggaagctct tggtgacgca
      841 agccaggtaa atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc
      901 caggttaacc cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg
      961 gaagtgatct ttcagcgtct tattacgctt ctcacggatc cagaactgcg agatgaccga
     1021 caggttaacg aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac
     1081 caggtcgaag ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg
     1141 agtacgccat ttcgggtgta cataaccgaa gaagcttttc gggaatacgc cgtcacgacc
     1201 cattacgtac atcagacgcg ctacgcctgc atgcgccgcc ataccggacg ccagtacggt
     1261 aatggtggag aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat
     1321 ttcaggctgt gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag
     1381 gaagtaagtt gcaaagatga agatcatgcc gccgatcagc gcggtcagga aaatcgcacg
     1441 cgggatcacg cgctctgcat ctttggtttc ttccgacagg ttgctgatgc cgtcaaagcc
     1501 ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc
     1561 agaccagaat ggacgagtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata
     1621 aacaaccatg cccagaatca ccgcgatcag caccacctgc aacacgacga ttacagtgtt
     1681 gaagttcgct acggatttca gactacgcag gttaaaggcg gtcatgaagg ccaccaacgc
     1741 caccacaaac atccacgatg ggatggaagg caccagagct tcaaaataga ttttcgccag
     1801 cagaatgttg atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa
     1861 gccgacagtc gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata
     1921 gcggcgaacc agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc
     1981 ataggctgtc ggcacatgac catccgtgag gcctgaaacg ataccaaaag tatcaaacag
     2041 cgtcatcggc tgcatatagg caagacccat cattacaacc ggaactaacg taagcgtttt
     2101 acgcaattcc acgcgagagg tgtttggagt aacgttatgc gacatggtca ttctccttta
     2161 cggtgataac cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa
     2221 caactgtcga tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg
     2281 attggtttga agcaaaaaat aaccgacgct gatgaaacgt cggtttttag tcattttttg
     2341 acagcggcgc attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata
     2401 ataatttttc tttaaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc
     2461 gcactatttg ctaaaatttc atcccgccac cagccactgg caagtcctgc ggtctgttca
     2521 ttccagccaa tccagacggg ttcaaagaga gtttgggcga ccactttctt ctcgattaac
     2581 gcgccactat cgagaaaatg ttgcgccaga taacggggta aatagccgca gccgaggccg
     2641 ctaatttgca gctccagctt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg
     2701 agaagttgtg aggccgtaga agcgcctgcc tgcgcagtat cccccaccac aatcgcccga
     2761 tatcgcttaa taatacgacg atttaacggc tcttcttcca gggctaacgg atgatgaggc
     2821 gcgaccgcaa agacttgttc cagatcgccc aaccgtgaaa aaccaaactc actggaagat
     2881 ggtggctcat gcatcgcgcc aacaataata tccgctcgcc cctgagtcag ggcatcccat
     2941 gaaccaccca gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa
     3001 gcttcaatca gtggcgctag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc
     3061 tcgttttccc agccttcatg cagtttgatc gcctgctttt ccagttcccg cacggtatgc
     3121 agaacttccc gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtga
     3181 ccgctacgat caagcaattg aatattaagg tcgctttcca gcttgtgaac ggtgtaactc
     3241 agagcggacg gcgtcttgta gagtttggcc gacgccgccg caaaactgcc ttctttttct
     3301 aaggcatcga ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgt
     3361 atgcggcgtc ctgctggcgt gatcgtttac tccagcggca ttaccagcgg atcgggatac
     3421 tggtattcaa atcccagttc attacaaatc cgactgccat caataatctt acctttgccg
     3481 ctgtccagac tatttctgaa ctgcggaggt tccagcccca gtaaacgggc catctgcgga
     3541 tagaaaacat tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta
     3601 ggtgcctgta acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca
     3661 ccatgttcac catcaggcgc ggtttttccg gcaaagaagc gtccgggatg acgccccggt
     3721 ccgaccaggc ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag
     3781 tcttcgagtt cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct
     3841 ttcaccatgc cttgcgcatc gccatagaca gatgttgagc tggtaaaaat aattcgggga
     3901 atacgatggg ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg
     3961 ccggagccgc tacgacgtgc cggaagcgta atgaccagtg catcggcatc catcagggca
     4021 tccagatcgt cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg
     4081 ccactcattc gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag
     4141 cctcgcgctg aaagtgacat cgccagcggc atgcctaacc accctaaccc gacaattgcg
     4201 acctttttca tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc
     4261 aaaactgaca attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat
     4321 gagcagtgaa tcgtcggtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga
     4381 ctagaaacct gacaaattat gtttcagggg ttctggcttc cccttacctt caaatggcgt
     4441 tctgcgggta tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg
     4501 ccagtacaga taatcatccc atgattcctc agaccagatt agtttcactc aataatgtcc
     4561 ttttctgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct ccgggcgtta
     4621 gcgggggaac gcagtagata agccgtctct tccagcgagt tgtattcttc gagtgacatc
     4681 agaacacaag cctctccatt ctgacgagta ataaggatcg gggcatgatc ttcaacggct
     4741 ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt acgcatgtca
     4801 atctcctctt ttgtacagtt cattgtacaa tgatgggcgt taattaacta tttattaatt
     4861 agtttgtaga tcaaggtatt gtcagtgaga cgaaaatcca ggcttcgcta tttttggtgc
     4921 cgtcagctta gaggacagtc ctcttagccc cctcctttcc ccgctcattc attaaacaaa
     4981 tccatcgcca tagaatatat aaaaaagccc ttgctttcta acgtgaaagt ggtttaggtt
     5041 aaaagacatc agttgaataa acattcacag agacttttat gacacgcgtt caatttaaac
     5101 accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg gaagacattc
     5161 agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc ttccgggggc
     5221 ttttttattg cgccgttgat aacggttcag acaggtttaa agaggaataa gaaaatgaca
     5281 gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga tgactcacgc
     5341 gaattgctgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct gatcgcgatg
     5401 gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg acgacattcc cggtctggta
     5461 atggacggcg tggttgacct cgggattatc ggcgaaaacg tgctggaaga agagctgctt
     5521 aaccgccgcg cccagggtga agatccacgc tactttaccc tgcgtcgtct ggatttcggc
     5581 ggctgccgac tttcactggc aacgccggtt gatgaagcct gggatggccc gctctcctta
     5641 aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct cgaccagaaa
     5701 ggtatctctt ttaaatcctg cttactgaac ggttctgttg aagtcgcccc gcgtgccgga
     5761 ctggcggatg cggtttgcga tctggtttcc accggtgcca cgctggaagc taacggcctg
     5821 cgcgaagtcg aagttatcta tcgctcgaaa gcctgcctga tacaacgcga tggcgaaatg
     5881 gaagaatcca aacagcaact gatcgacaaa ctgctgaccc gtattcaggg tgttatccag
     5941 gcgcgtgaat caaaatacat catgatgcac gcaccgaccg aacgtctgga tgaagtcatc
     6001 gccctgctgc caggtgccga acgcccaact attctaccgc tggcgggtga tcaacagcgc
     6061 gtagcgatgc acatggtcag tagcgaaacc ctgttctggg aaacgatgga aaaactgaaa
     6121 gcgctgggtg ccagttcaat cctggtcctg ccgattgaga agatgatgga gtgatcgcca
     6181 tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa cgccagctgt
     6241 taatgcgccc ggcgatctcc gcttctgaaa gcattacccg cactgttaac gatattctcg
     6301 ataacgtgaa agcacgcggc gatgaggccc tgcgggaata cagcgcgaag tttgataaaa
     6361 ccacggttac cgcactgaag gtgtctgctg aggaaattgc cgccgctagc gaacgcctga
     6421 gcgacgagct aaaacaggcg atggcggtgg cagtgaagaa tattgaaacc ttccacactg
     6481 cgcaaaaact gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc cagcaggtca
     6541 cgcgcccggt cgcttcagtt gggttgtata ttcctggcgg ctccgccccg ctcttctcaa
     6601 cggtattaat gctggcgact ccggcgcgta ttgcgggctg taaaaaagtg gtgctgtgct
     6661 caccgccgcc gattgccgat gagatccttt atgcggcgca gctgtgcggt gtgcaggacg
     6721 tgtttaacgt cggcggcgca caggccattg ccgcactggc gtttggtacg gaatctgtgc
     6781 cgaaagtgga caaaatcttc gggccgggca acgcctttgt caccgaagcg aaacgtcagg
     6841 tgagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg gaagtgttgg
     6901 tcattgccga cagtggcgca acgccggatt tcgtggcttc tgatttgctt tctcaggctg
     6961 aacacggccc ggactcacag gtgattttac tgacgcccgc tgctgatatg gcgcgtcgcg
     7021 ttgccgaggc tgtcgaacgc cagctggcag aactgccgcg tgctgaaacc gcccgtcagg
     7081 cactgaacgc cagccgcctg atcgtgacta aagatttagc gcagtgcgta gagatctcca
     7141 accagtacgg cccggagcac ctgatcatcc agacccgcaa cgcccgcgat ctggtcgatg
     7201 gcattaccag tgccggttcg gtatttcttg gtgactggtc actggaatcg gcaggtgatt
     7261 acgcctccgg caccaaccac gttctgccga cttacggtta caccgccacc tgttccagcc
     7321 tcgggctggc agatttccag aagcgcatga ccgtgcagga actgtcgaaa gaaggtttct
     7381 ccgcgctggc ttcgaccatt gaaacattgg ccgccgccga gcgcctgacc gcccataaaa
     7441 atgccgttac tttacgtgtt aacgccctta aggagcaagc atgagcatcg tgactattac
     7501 cgatttagcg cgtgaaaacg tgcgcaacct gacgccgtat cagtcggcgc gtcgtctggg
     7561 cggtaacggc gatgtctggc tgaacgccaa cgaatatccc acagccgtgg aatttcagct
     7621 tactcagcaa acgctcaacc gctacccgga atgtcagccg aaagcggtga ttgaaaatta
     7681 cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agccgtggcg cggacgaagg
     7741 tattgaacta ctgattcgcg ctttttgcga accgggtaaa gacgccatcc tctactgccc
     7801 gccaacgtac ggcatgtacg gcgtcagcgc cgaaaccatt ggcgtcgagt gccgcacagt
     7861 gccgacgctg gacaactggc aactggattt acagggcatt tccgacaagc tggaaggcgt
     7921 aaaagtggtc tatgtttgca gccccaacaa cccgaccggg caactgatca atccgcagga
     7981 ttttcgcact ctgctggagt taacccgcgg taaagcgatt gtggttgccg atgaagccta
     8041 tatcgagttt tgcccgcagg catcgctggc tggctggctg gcggaatatc cgcacctggc
     8101 tattttgcgc acactgtcga aagcttttgc tctggcgggc cttcgttgcg gatttacgct
     8161 ggcaaacgaa gaagtcatca acctgctgat gaaagtgatt gccccctacc cgctctcgac
     8221 gccggttgcc gacattgcgg cccaggcgtt aagcccgcag gggatcgtcg ccatgcgtga
     8281 gcgggtggcg caaattattg cagaacgcga atgcctgatt gccgcactga aagagatccc
     8341 ctgcgtggag caggttttcg actctgaaac caactacatt ctggcgcgct ttaaagcctc
     8401 cagtgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc agaataaaca
     8461 accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa gccagcgcgt
     8521 cattgacgcc ttacgtgcgg agcaagttta atgagtcaga agtatctttt tatcgatcgc
     8581 gatggaaccc tgattagcga accgccgagt gattttcagg tggatcgttt tgacaaactc
     8641 gcctttgaac cgggcgtgat cccggaactg ctgaagctgc aaaaagcggg ctacaaactg
     8701 gtgatgatca ctaatcagga tggtcttgga acacaaagtt tcccgcaggc ggattttgat
     8761 ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtgcagtt tgatgaagtg
     8821 ctgatttgcc cgcacctgcc cgccgatgaa tgcgactgcc gtaagccgaa agtaaaactg
     8881 gtggagcgtt atctcgctga gcaagcgata gatcgcgcca acagttatgt gattggcgat
     8941 cgcgcgaccg atattcaact agcagaaaac atgggcatta ctggtttacg ctacgaccgc
     9001 gaaaccctga actggccgat gattggcgag caacttacca aacgagaccg ttacgcccat
     9061 gtagtgcgca acaccaaaga gacgcagatt gacgttcagg tgtggctgga tcgcgaaggt
     9121 ggcagcaaga ttaacaccgg cgttggcttc tttgatcata tgctggatca gatcgccacc
     9181 cacggcggtt tccgcatgga aatcaacgtc aaaggcgacc tctatatcga cgatcaccac
     9241 accgtcgaag ataccggcct ggcgctgggt gaagcgttaa aaattgccct cggcgataaa
     9301 cgcggtattt gtcgctttgg ttttgtgctg ccgatggacg aatgccttgc ccgctgcgca
     9361 ctggatatct ctggtcgccc gcacctggaa tataaagctg aatttaccta ccagcgcgtg
     9421 ggcgatctca gcaccgagat gatcgagcac ttcttccgtt cgctctctta caccatgggc
     9481 gtgaccctgc acctgaaaac caaaggtaaa aacgatcacc accgtgtaga gagcctgttc
     9541 aaagcttttg gtcgcaccct gcgccaggcc atccgcgtgg aaggcgatac cctgccctcg
     9601 tcgaaaggag tgctgtaatg aacgtggtga tccttgatac tggctgcgcc aacctgaact
     9661 cggtgaagtc tgccattgcg cgccacggtt atgaacccaa agtcagccgt gacccggacg
     9721 tcgtgttgct ggccgataaa ctatttttac ccggcgttgg caccgcacaa gcagcgatgg
     9781 atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa ccggtgctag
     9841 gcatctgctt agggatgcaa ctgctggggc ggcgcagcga agagagcaac ggcgtcgatt
     9901 tgctgggcat catcgacgaa gacgtgccga aaatgaccga ctttggtcta ccgctgcccc
     9961 atatgggctg gaaccgcgtt tacccgcagg caggcaaccg cctgtttcag gggattgaag
    10021 acggcgcgta cttttacttt gttcacagct acgcaatgcc agtcaatccg tggaccatcg
    10081 cccagtgtaa ttacggcgaa ccgttcaccg cggcggtaca aaaagataac ttctacggcg
    10141 tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa aacttcctgg
    10201 agatgtgatg attattccgg cattagattt aatcgacggc accgtggtgc gtctccatca
    10261 gggcgattac ggcaaacagc gcgattacgg taacgacccg ctgccgcgct tacaggatta
    10321 cgccacgcag ggtgccgaag tgttgcatct ggtggatctg accggggcaa aagatccagc
    10381 aaaacgtcaa atcccgttga ttaaaaccct ggtcgcgggc gttaacgttc cggtgcaggt
    10441 tggcggcggc gtgcgtagcg aagaagacgt ggcggcgtta ctggaagcgg gcgttgcgcg
    10501 cgtggtggtc ggctccaccg cggtgaaatc accagaaatg gtgaaaggct ggtttgaacg
    10561 tttcggtgcc gatgccttag tgctggcgct ggatgtccgt attgacgagc aaggcaacaa
    10621 gcaggtggca gtcagcggct ggcaagagaa ctcgggcgtt tcactggaac aactggtgga
    10681 aacctatctg cccgtcggcc tgaaacatgt gctgtgtacc gatatctcgc gcgacggcac
    10741 gctggcaggt tctaacgtct ctttatatga agaagtgtgc gccagatatc cgcaggtggc
    10801 atttcagtca tccggcggca ttggcgacat taacgatgtt gctgccctgc gtggcactgg
    10861 cgtgcgcggc gtaatagttg gtcgggcatt attggaaggt aaattcaccg tgaaggaggc
    10921 catcgcatgc tggcaaaacg cataattccg tgtctcgacg ttcgtgatgg tcaggtggtg
    10981 aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccgct ggcaaaacgc
    11041 tacgccgaag aaggtgcaga cgaactggtg ttctacgata tcaccgcttc cagcgatggc
    11101 cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat tccgttctgc
    11161 gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa ttctttcctt tggcgcggat
    11221 aaaatttcca tcaactcgcc tgcgctggcg gatccgacgt taattactcg cctggcggat
    11281 cgttttggcg tgcagtgtat tgtggtgggt attgatacct ggtacgacgc tgaaaccggt
    11341 aaatatcatg tgaatcaata taccggcgat gaaagccgca cccgcgtcac tcagtgggaa
    11401 acgctcgact gggtacagga agtgcaaaaa cgcggtgccg gagaagtcgt cctcaacatg
    11461 atgaatcagg acggcgtgcg taacggttac gacctcgaac aactgaaaaa agtgcgtgaa
    11521 gtttgccacg tcccgctaat tgcctccggt ggcgcgggca ccatggaaca cttcctcgaa
    11581 gccttccgtg atgccgacgt tgacggcgcg ctggcagctt ccgtattcca caaacaaata
    11641 atcaatattg gtgaattaaa agcgtacctg gcaacacagg gcgtggagat caggatatgt
    11701 taacagaaca acaacgtcgc gaactggact gggaaaaaac cgacggactt atgccggtga
    11761 ttgtgcaaca cgcggtatcc ggcgaagtgt taatgctggg ctatatgaac ccggaagcct
    11821 tagacaaaac catagaaagc ggcaaagtcg ccttcttctc gcgcactaaa cagcgactgt
    11881 ggaccaaagg cgaaacgtcg ggcaatttcc tcaatgtggt gagtattgca ccggactgcg
    11941 acaacgacac gttactgtta ctggtgaatc ctatcggacc aacctgccat aaaggcacca
    12001 gcagctgctt cggcgacacc gctcaccagt ggctgttcct gtatcaactg gaacaactgc
    12061 tcgccgaacg caaatctgcc gacccggaaa cctcctacac cgccaaactg tatgccagcg
    12121 gcaccaaacg cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca ctggcagcga
    12181 cagtacatga ccgctttgag ctcaccaacg aggcgtctga tttgatgtat cacctgctgg
    12241 tgctgttgca ggatcaggat ctggacttaa cgacggtgat tgagaacctg cgtaaacggc
    12301 atcagtgagt tgcggggtaa gcggatgcga tattgttgta gcatccggaa aaaaaaacgg
    12361 gcaaggtgtc accaccctgc cccttttctt taaaaccgaa aagattactt cgcgttgtaa
    12421 ttacgcagag cgttacgccc caagacaatc cccgcgccaa ccatgccgcc cagcagcacc
    12481 gccagaatca aggtaattgc ctttttcggg ctatcgcgac gaataggtaa cgtcggtttc
    12541 attacatagc ggtaagcatg aatatcaagt ttatcaacgt caagattgtc gatatccagc
    12601 aggttttgac gagtctgata gtagtttggt gagaacacca acggacgagt cgcttcatgc
    12661 ttaatcatcg actccagcgc ttcgctcccc aaaaggaaca acgtatcttg tgtcacatct
    12721 tcagtctgct gaacctgtgg ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc
    12781 tgaatctgac ggatacgcag atctttctgc tcctgcgcaa ctacttcctg agttttcaga
    12841 gaatcctgca agtttttcat acgcagcgcg atgttgtctt tcaggtcttt tcccagttct
    12901 tcatttacct ggtcgtcaac ttgctggata tacttcgcca gctgcttctg agcaccttca
    12961 ggtgtttcac caacataaga caccgctaaa ggtaatgact gatttttgac ggtaggctca
    13021 atggtcagct tttctgcttc ttcctggtta tccagcgttt ccgctaatgc tgagaatgca
    13081 gtgctgtaac gtccgataag tgaagcctga atgtcggaga ctttcggtac agccgatccg
    13141 tagatcacat tcatcgcgtt ggtataacca gcgatttgcc ccacatcagg ttgagtaacg
    13201 atagctgtgg aagtccactt ttctttggca acggccagat accctatagc cagggcaata
    13261 gcaacaatga cagagatgat aattgtcatc ttgccacgcc acaactgcac cagtaaatca
    13321 atcaaatcaa tctgttccgg atcatggttt tgcccagaaa cattattatt ttctactctc
    13381 atcattaccc taacggacga aataatttta agtcgccata gtttagcgat aatcgccatg
    13441 aatgctatag gataaatgat aaaaataatg aattattaca aagggaacat aagagaatca
    13501 gggcgaaaat cgccatgata acaggatgtt agtcactgcc aaagagatcg cgggtgtaga
    13561 ctttgtctgc cacatcctta agctcttctg ccatacggtt ggagataatg acgtcggctt
    13621 gtatagtcag cttagaacag ctttgattgt aaaatgagca cgttaccaga aaaaacagcc
    13681 aagtttagaa ctgataccga ttatcttttt ttcttgtcta acggtataat ttaactttca
    13741 gttatgccag atgaagattg gctatattct agcctgagcg aggattataa atgaaatttc
    13801 tggttacggg agctgctggc tttatcggtt tctatctaag taaacggctt cttgcagctg
    13861 gtcatcaggt tgtaggcatt gacaacttaa atgattatta cgatgtcaac ctcaaacaag
    13921 cacgacttga tttactcaag cacgacaact tcagttttta taaaattgac ctggccgatc
    13981 gtgagaaaat ggcggcactg tttgcagacg agcggttcga acgcgtaata aacctcgctg
    14041 cgcaagccgg tgtacgttac tctcttgaaa atcccaatgc atatgcagat gcaaacctga
    14101 ttggattcct gaacatacta gaaggatgtc gccataataa tgttcagcat ctactttacg
    14161 cttcctccag ttctgtttat ggcatgaacc gcaagatgcc tttctctaca gatgactctg
    14221 tagatcatcc tgtttcgctt tatgcagcaa ctaaaaaagc gaatgaactc atggcccata
    14281 catattctca tttgtatggc ttaccgacca cagggctgcg tttctttacg gtttatggtc
    14341 catggggacg tccggatatg gcattattta aattcactaa agccatgctg gaaggtaaaa
    14401 gcattgatgt ttacaacttc ggcaaaatga agcgtgactt tacttacatt gatgatattg
    14461 ccgaagctat tattcgctta caggatgtta ttccagaaaa aaacccacag tgggctgtag
    14521 aaacaggctc gcctgcaaca agttcagcac catatcgtgt ctataacatt ggtaatagtt
    14581 cgcctgtgga gttgatggac tatatcaatg cgttagaaga ggctctgggt attgaagcca
    14641 acaaaaatat gatgcctctc caacccggtg atgtactgga aaccagtgct gatacaaaag
    14701 cactgtatga cgtaatagga ttcaaacctg aaacgtcagt taaagaaggg gtaaagaact
    14761 ttgtagaatg gtatcgtaac ttctataaag tttgatttta caaaaccata agaaaaggcc
    14821 ctaatttatt agggcctttt cttagaatga aacaaaataa ttaatcattg ccaaacaagt
    14881 cgcgcgtata aactttatct gctacatcag ccagatcggc agacatacgg ttagaaataa
    14941 taacatcagc ttcttgtttg aacgcatcca gatcacgtac cacgcgcgac cggaaaaaat
    15001 cgtcctcttt catagctggc tcataaacga ttacaggcac acctttcgcc ttgattcgct
    15061 tcataatacc ctgaatcgag gaagcacgaa aattgtctga accattcttc ataatcaaac
    15121 gatagacgcc aacaactttc ggtttacgtg caaggataga atcggcaata aaatctttgc
    15181 gcgtgcggtt ggcgtcaaca attgccgaga tcaggttatt cggcacagac tggtaatttg
    15241 ccagtaactg cttagtatct ttcggcagac aataaccacc ataaccgaat gacgggttgt
    15301 tgtagtgatt accgatacgc gggtcaaggc atacgccctc aataatctgg cgtgaattaa
    15361 gtcccaggct ttcagcataa ctatcaagtt cattgaaata cgctacacgc atcgccagat
    15421 aagtgttcgc aaaaagttta atcgcctcag cctcggttga gtcagtaaac aatgttggta
    15481 tgtcttgctt aatggcgcct tcctgtaata acgcagcaaa acgtttagcg cgttcagact
    15541 gctcgccaat cacaatgcgt gatgggtgta agttatcata aagtgcttta ccttcacgca
    15601 aaaactcagg cgaaaagatc acattttcaa taccaaaacg ttctttaatg gactctgtaa
    15661 aaccaacagg gatagttgat tttataatca ttaccgcgtt gggattaatt tctgtcacat
    15721 cacgaatgac cgcttccacg cttgaggtat taaaataatt tgttttcgga tcataatcgg
    15781 taggtgtggc aataataacg taatcggcat ttttatacgc gtcatactta tctgtcgtag
    15841 cgcggaaatt gagatcttta gtcgccagat actcttcaat ctccttatca acaagcggtg
    15901 actgcctctt gttaagcatg tccactttgg cctgaacgat atccagtgca accacttcgt
    15961 ggttttgcgc aatcagaata ccatttgaaa gaccaacata acctgttcct gaaattgtta
    16021 ttttcattag ctctgacttc ttccggttaa acatttagag tggtcattaa tccaccacca
    16081 cagcttcatc tactgcggga tttttaacgc tgatgtagat gaactgtcaa ggcagcgatc
    16141 ctgctgtgcg gcgctgtatt atatcgcgtt tttaactata aattataaaa aaaggcccta
    16201 acctgccgct ttgtataata aaaaagcccg gagggtttct ccgggccttg ctttgattaa
    16261 ttgatttaaa tcagattaat ccagccattc ggtatggaac acaccttctt tatcaatgcg
    16321 cttataagta tgcgcaccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac
    16381 agcggcgcgg tagctgtcgt aataggcaac cgcagcggcg aaggtcggca ccgggatacc
    16441 gttctgtact gcgtaagcga cgacatcgcg cagcgcctgc tggtagtcat cggcaatttg
    16501 cttgaagtaa ggagccagca acaggttagc gatctgcgga ttttcggcat aagcatcggt
    16561 gattttctgc aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc
    16621 accgtagttc agatcccagt tgtactcttc agacgcagcg cgtagctgag agaagccctg
    16681 agcgtaagaa acgattttgc ccagatacag cgcacggcga actttttcga tgaactcagc
    16741 attgtcgcca gctggctgcg cttgcgggcc agagagaact ttagatgcgg caacacgctg
    16801 ctctttcaga gaagagatat aacgtgcaaa cacagactcg gtaatcagcg acagcggttc
    16861 gccgagatcc agcgcgctct ggctggtcca tttgcccgta cctttgtttg ctgcttcatc
    16921 cagaatcaca tcaaccaggt agttaccctc ttcatctttt ttggtgaaga tatctttggt
    16981 gatgtcgatc aggtagctgc tcagttcacc gttattccac tcggtaaagg tctgcgccag
    17041 ttcttcgttg gtgaggttca agccaccttt aagcagagaa taggcttcag caatcagctg
    17101 catatcaccg tattcaatac cgttgtgaac catcttcaca taatgacctg caccatcggc
    17161 accaatatag gtaacgcacg gttcgccgtc ttcagccaca gcggcgattt tggtcaggat
    17221 cggcgcaatc agttcataag cttctttctg cccaccaggc ataatggaag gacctttcag
    17281 cgcaccttct tcaccaccgg aaacaccggt accgataaag ttaaagcctt ctgcagaaag
    17341 ctcacggtta cgacgaatgg tgtcatggaa gaaggtgtta ccaccatcaa tgatgatgtc
    17401 acctttatcg aggtatggct tgagggaatc aatagcagca tccgtgccag cacctgcttt
    17461 caccattaac aggatgcgac gaggcgtttc cagagattca acaaattctt tcaccgtata
    17521 gtaaggaacc agtttcttgc ctggattttc ggtaatcact tcttcggtct tttcacggga
    17581 acggttgaaa atagagacgg tataaccacg gctttcgata ttgagcgcaa ggttgcgccc
    17641 catcactgcc ataccgacga cgccgatctg ttgctttgac attgtttact cctgtcagga
    17701 taccgctggg tggtatgcgg gttatgctta attatagaat atgcctaata aaaataaatc
    17761 cataacactt aatcagaaaa ttattattat cgattcctaa cgattgaata catcagctcc
    17821 tttaatttag atggcattat acgaaaaaat gttctcaaca tagcattact tattaattca
    17881 ttttttcgaa taaaaccaat tttatattga taatacaata ctttatactc gtacaataaa
    17941 tatgacaatc cacgtcgagc cataagatta cgaccagttc gcatttttaa taaaatatct
    18001 ggaagatttg caaatcttgc attatgtaca attaataggc tccacaatgc aaaatcttga
    18061 gattttctga atggaggata accaccaaca gctaatactg tattctttct aaaaattaca
    18121 gaaggatggc taactgcgct tcgtttcctc gcgaatttaa ctatttctct atgttcgaga
    18181 ggcactttgc gtgttgaaat aaactcctca gtaacagttt caatttcatc aataaaactg
    18241 ccacatacat ctatttctga attattaatc ataaaagaaa tttgtttctc aaaccgatga
    18301 ggcaaagaaa tatcatcagc atccattctt gccactaact cattcctaca agcctttaat
    18361 ccttcattta aggcattagc caatccaaca tttctaggta aaggtacaaa tgttactatt
    18421 ttattgccaa catcatcaat gaatgaattt ataatatcga tgtgtgtttg atggagttct
    18481 ccatctgcaa caattactat ttgatctggc ttaagtgttt gatcgtgaaa aatagagcgt
    18541 agagccacct caaaaaattg cggtagatca tttttataaa tgctaattaa aactgagaat
    18601 ttttctaatc tatgattcat ttcattttac cacttcgacc cattaaaccg tcattaatgc
    18661 cttttaaaaa aaaatataac cttttattac catttggaag gaaaatagga tataaaaaaa
    18721 cctttccaat taatttaacc agactagaaa ttttccagta gatgggtaca taatttttat
    18781 ttaataaaag aaagatattt cgagtagcat aataatgacg aaatgggctt ggcaaaccga
    18841 cagaaagaat atttaagatc ttaaatcgcc catctccaag tctatgtgca agtaacgcat
    18901 ttttattcct aattacttta aacccagcag ctcttaatct ccaacaatat tcatggtcta
    18961 ccgcatcgat aaaaagctca tctttcattc ctccaacaat caaccaacta ttttttggta
    19021 ttagactgcc agaacttaat gtactatcta cctcataata aacttctgta agtggtttcc
    19081 ctttttttac ccttgcttta tttaattcac cagttacttt atcaaaatct tgtgaaccaa
    19141 ctaaaccaac attgacattt tgtttaagca attttttgta acaagtaagt aactgctcta
    19201 ccatcttagg atcaggaata ctatcctgat ccatttgcaa tataaaatca gcgccatttt
    19261 caaaagccca tttcattcct atactttggg cttctgctat gcctaaatta tcattgaaat
    19321 tgaatatttt tacatcgcct gaagaatttt cagcatattt ataaccattt gtagagttat
    19381 tgcaaacgac aactttagta acttgtctca acaataattc aaccgcattt tttaaatcat
    19441 tatgttctgg gttgtaagca accaaaacgg catatacagt gtccatcttc accttaaaac
    19501 cttcatttgg ctttcatctt ttttagaaca ttacttaatg tcactaatac aattattaca
    19561 gcaacatggt tagagtctaa aatataagga ttagtaattg cataagaaac atatagaaaa
    19621 tatagcacac acaactcact gtattttatg attttaatcg tgagaaggag attaattaat
    19681 aaaaacaaag taaataaaat aacgccaagt tgatttaaaa aataaactga ctgcaattca
    19741 taatatatat atgcactata atcacggata ggagtttgaa ttttgatgac attacccaaa
    19801 ccagaaccta taacaaaatt tgatacagac tctgtaagat cattaattaa tacagtaaac
    19861 tgatcccatc taactcctaa agaagaatca gctccatttg atttcatgat tatcaactca
    19921 attgaatatg taataaaaaa agggagaatc acagtaagaa aaaccccaaa aataattttc
    19981 cttaatttag cgtatcgtga gttagattta gaacatagta taatatacat aaaaaacaag
    20041 catatcgaaa caaaatatgc aaaattacca gccactatag tacctatagc cagaataacg
    20101 gttattgtat ttttgaatcg ataatagaaa taatctttta tgactatatg caacataaag
    20161 gcaaatggaa tgagagcatt tcctttaatt tgaactctat agaaaccact tccatatgta
    20221 taaacatcac cataatcatt ctccaaaaaa taatgtctta gtgctgaata atcaccaata
    20281 ccatatgttt ttgtcatata aatactaatg atggatataa taaccgcctg taataccatt
    20341 aaatataaaa atattttaac aatcgagatg gttccataag agcagaaata agcacataat
    20401 ataaataata tgataatata aaacctaatt attatcgcta tatcgttacc cttgatatag
    20461 gaataaataa aatttataaa aagagctaat agaaatatta aaataacagg atagtgatat
    20521 attccgtttg caattttctt tgtaaatgac atgatacaaa gacataaaaa cccctccata
    20581 atccaactat attgaataaa tggaaagcta cgtgtaagga aaaatataaa cccaaaaaac
    20641 aaaagaacac ttaaactttt gtcttttgag ttataaaaat cagaagtcat gtttgcactc
    20701 taattagatg ggcttgagga agtaatccct aaaatcaatt cgctattaat atttcgtatc
    20761 aattaataat aatatcaaaa aatctaacga tgttcttaca gaccatgcta ttgcggctcc
    20821 aacaattccc caatgataaa taaaaatata taatatgcat aaatatggga taacttcgag
    20881 caaatgaata atagctgtaa tttttgatct tccactagcc tgaactgaaa caaatgggat
    20941 ttgtgcaatg caattaaaaa agaaacctat tgcaagaatt tttaatacta tacctggcgt
    21001 cccatgatat gtaggtccca tccaagcgga cattataaaa tctgataaaa taattatcaa
    21061 cattacaatt ggaagtatac caataaccat tataaaatat gataatattt tagtttgctt
    21121 taccgattgc aattctgaac ttaatcttgg aaaaatagct ctggacaacg cacttggtaa
    21181 tatcgttaag cgttgtatac cttcagacgg agcagtataa aaagaaactt tatcagcccc
    21241 cacaatgtgt gaaagaataa aacgatccat atatgtcata atagggctaa taatattgct
    21301 aactgttatc cagcttccaa agccgattaa tcttttaact gttacaattt ttacagacag
    21361 cccagatgat attattagtt ttcgactaaa tataaaggtc actataagtg ataagactct
    21421 tgccataact aaaccatata tagcacttag taatcctcca tgaaaaaaac agaaaatcac
    21481 tggtaatcca gccacaaaag agttgttaat tgattttatt aaatttactt ttctgaactt
    21541 ttccatcccc tcaaaaatcc ccaaccagac ttggtttaac aagtataagg gtatggtagc
    21601 tgaaataata tatattgctt tgacagattc tacaacataa ttcgcgttaa tgtttaataa
    21661 tttaacaatt acattgctac tcaaaaatag tacactaccg ccaatcaagc ccaatatagt
    21721 tagaattacc gttgaagttg aaatgatcgc tcttaattct ttatgaacat ttttatatat
    21781 tgatacttct cttataacag ctctggtcaa tccagcatca aaaatacttg catatccaac
    21841 taaggcaata gctaacgtaa aaaggccaaa ttgctcggtc cctagaattc tagacagtat
    21901 acctaacgca ggaattgcta ttaatgatgg tataatatac ccacttatat tccataaagt
    21961 attctttaca atactcacaa aaataattcc ttcatgttat gcaattcttt agcccttgca
    22021 tctttaatcg ataaaatata attattatgt tctatcgtcg gccattttat gctcagaata
    22081 ggatcattcc atacaatccc tctatcacta tcaggatgat aatagttcgt cgttttatat
    22141 aaaaattccg cagtctcgct cagcaccaaa aaaccatgtg caaatccctc agggatccac
    22201 aattgccgct tattctcagc agataaattc accccaaccc atttaccaaa ggtaggcgac
    22261 gatttacgaa tatcaacagc tacatcaaaa acctcaccaa caacgcaacg taccagtttc
    22321 ccttgcgcat aaggttctaa ctgataatgc agcccgcgta aaacaccttt actagacttc
    22381 gaatggttat cctgaacaaa ttcaacctta cgtcctacag cttcttcgaa aactttctga
    22441 ttaaagcttt ccataaagaa accacgctca tcaccaaaaa ctttcggctc gaaaattaac
    22501 acatcaggaa tttctgtttt aattacgttc attttattaa taacctttaa tcattttcag
    22561 cagatactgt ccataagcat tttttttcag cggctccgct aatgctttca cctgttcagc
    22621 atcaataaac cctttacggt aagcaatttc ttctgggcag gaaaccttta gtccctggcg
    22681 ctcttcaatg gtggcaatga agttgcttgc ttcaataaga ctctgatgtg tccccgtatc
    22741 cagccatgca taaccacgcc ccatcatggc aacggataaa cgcccctgtt ccatataaat
    22801 acggttaata tcggtaattt ccagttcacc acgggcagaa ggcttaaggt ttttcgccat
    22861 ttcgacaacg tcgttatcat agaaataaag cccggttacc gcataattac tttttggttg
    22921 tagcggtttt tcttccaggc ttattgccgt accgttttta tcaaactcaa cgacgccgta
    22981 gcgttcagga tcattaacgt gataggcaaa taccgttgca ccactttctt tgttaacagc
    23041 gacatccatt aacttcggca gatcatgacc gtagaagata ttatcaccaa gaaccaaagc
    23101 acaatcatca ccaccgataa actcttcacc gataataaac gcctgcgcaa gcccatctgg
    23161 agtcggttgc actttgtact gaagatttag cccccactgg ctaccgtcac ctagcagttg
    23221 ttgaaaacga ggagtatcct gtggcgtact aataatcaga atatcgcgaa tacccgccaa
    23281 catcagtgta gagagcgggt aatagatcat cggcttatca taaataggta atagctgttt
    23341 actgacagcc atagtcacag gataaagacg tgtaccagaa ccacccgcta aaataatacc
    23401 tttacgcgtt ttcatttcat cattcctttt aattcatctt gctccaccat cacgaacaag
    23461 atgcaaaaac tattaaattg ctgtagtcgt aattaattcg ttgagcattc gtttcacacc
    23521 aacctgccag tcaggcaaga caagcgcaaa gttctgctga aatttttctg tattaaggcg
    23581 agagttatgt ggacgacgag ctggtgtagg ataggctgtt gttggtactg cgttgagctt
    23641 gttgagtgca aggggaatac ctgctttgcg cgcctcttca aaaaccagcg cagcataatc
    23701 gtgccaggtt gtggtaccac tggctaccag atggtacaaa cctgcgactt ccggtttatt
    23761 cagtgccaca cgaatagcat gtgccgtaca atcagccagc agctcagcac ctgttggcgc
    23821 accaaattga tcatttatca cagccagttc ttcgcgctct tttgccagac gcaacatcgt
    23881 tttggcgaag ttatttcctt tagctgcgta tacccagctg gtacggaaaa taagatgctt
    23941 cgcgcaatgt tcctgtaacg ctttttctcc ggctaacttg gtttcaccgt aaacatttag
    24001 cggtgcggtt gcatccgtct ccagccatgg cgtgtcgcca tttccaggga atacgtagtc
    24061 agttgagtaa tgaattaccc aagccccaac ttcattagcc tcttttgcaa ttgattcaac
    24121 actagtcgca ttgagtaatt gtgcaaattc gggttctgac tcagccttat ctactgcggt
    24181 gtgagccgca gcattaacaa taacatcagg tcgaattctt ttgactgttt cagctacacc
    24241 ttcaggatta ctaaaatcac cacaataatc agtggagtga acatcaagag caatcaaatt
    24301 acccaaaggt gccagagcac gctgtagttc ccaacctacc tgccctgttt tgccgaaaag
    24361 gaggatattc attactggcg gccctcatag ttctgttcaa tccacgattg ataagcacca
    24421 cttttcacat tatcaaccca ttttgtattg gacaggtacc attccaatgt cttccgaatc
    24481 ccgctctcaa acgtttcctg cggtttccag cccaattcgc ggctaatctt ctctgcatca
    24541 atcgcataac ggcgatcgtg tcccgggcga tcggcaacat aagtaatttg ctcgcggtaa
    24601 gatttctctt tcggtacaat ctcatccagc aaatcacaaa tagtgagcac tacatcgatg
    24661 tttttctttt cgttgtgtcc accaatgtta taagtttcac ccgctttacc ttcggttacg
    24721 acggtatata acgcacgcgc atgatcttca acatacagcc agtcacgaat ttgatcccct
    24781 ttgccataaa taggtaatgc cttaccttcc agagcattca gaataaccaa tggaatcaat
    24841 ttttccggga aatgataagg accataatta ttagagcaat tagtcacaat ggttggtaaa
    24901 ccataggtac gtttccacgc gcggactaaa tgatcgctgg atgcttttga agcggaataa
    24961 gggctgcttg gcgcgtaagc tgttgtctct gtaaataagg gtaattcttc tgtattattt
    25021 acctcgtcag gatgaggcaa atcaccatag acttcgtcag tagaaatatg atgaaaacgg
    25081 aatctagttt tcttgtcgct atcaagagca gaccaataat tgcgagcggc ttccaaaagg
    25141 acatatgtac caacaatatt ggtttcaata aatgccgcag gacctgtaat tgaacggtca
    25201 acatggcttt cagcagccag gtgcatcact gcatctggct gatgctgagc aaaaatccgt
    25261 gccattgcag ctgcatcgca aatatccgca tgttcaaaaa catagcgttc agaatcagaa
    25321 acatcagcaa gtgattccag gtttccggcg tacgttaatt tatcgacatt aacaacacta
    25381 tcctgcgtat tatttataat gtgacgaact acagcagaac caataaatcc tgcgccacca
    25441 gtaacaagta ttttcaccta atttattcca tattgcttca gagcatgctg tgaaataagc
    25501 ggctctcagt ttgattaata gaagtattaa tgcacgctac cgcccctggc tttacagcta
    25561 ccagagcact gcatgcatgc ctacgatgtg acgagcgtta cccactcgcg ctaaacccga
    25621 aaaattcaaa agctaattgt cttaccaatc cgctctggaa acaaggaaaa tcctggaaaa
    25681 ctttgactaa aatcctattg ctaactcgtt gttattctga ttgtttatat aaaacaacgg
    25741 caggaatatt cgcaacaaat tactttcacc acgaatcttc actgccgtta taattttctt
    25801 atcaaccgtt acatccggtc agattttcat tattcgctta acagcttctc aataccttta
    25861 cggaacttcg ccccttcttt caggttgcgc agcccatact tcacaaacgc ctgcatatag
    25921 cccatttttt taccgcagtc gtagctgtcg ccggtcatca gcattgcatc aacggactgt
    25981 tttttcgcca gctcggcaat ggcatcagtc agctgaatac gtccccatgc accaggctga
    26041 gtacgttcaa gttccggcca aatatcggca gaaagcacat agcgaccaac ggccatgatg
    26101 tctgagtcca gcgtctgcgg ctgatccggt ttttcgataa attcaacaat gcggctgact
    26161 ttaccttcgc gatccagcgg ttctttggtc tggatgacgg agtattcaga gaggtcaccc
    26221 ggcatacgtt ttgccagcac ctggctacgg cccgtttcat tgaagcgcgc aatcatggca
    26281 gcaaggttgt agcgtagcgg gtcggcgctg gcgtcgtcga tcacaacgtc tggcagcacc
    26341 acgacaaatg gattgtcacc aatggcgggt cgtgcacaca aaatggagtg acctaaacct
    26401 aaaggttcgc cctgacgcac gttcataata gtcacgcccg gcgggcagat agattgcact
    26461 tccgccagta gttgacgctt cacgcgctgc tcaaggagag attctaattc ataagaggtg
    26521 tcgaagtggt tttcgaccgc gttcttggac gcatgagtta ccaggaggat ttctttgatc
    26581 cctgcagcca caatctcgtc aacaatgtac tgaatcattg gcttgtcgac gatcggtagc
    26641 atctctttgg gtatcgcctt agtggcaggc aacatatgca tcccaagacc cgctaccggt
    26701 ataactgctt ttaaattcgt cattattttc ctacctctaa ggggctgata gtgcgtaaat
    26761 tattgtcata ggttagccaa acggtatggc tatataccaa gcataacttt gattaaacct
    26821 tacgataaca ctacacacca tcagcatctg ggttactcgg attactcgga aatccacata
    26881 ctgataattt aatcagtacc tctttccgaa taatcgtagt ccaacctggt ccttttttct
    26941 ctgactcgtc tgcattactc agaaacaaac gttatgtcgt cttttttggc atggacgaat
    27001 tcatactgca gagttcgatc cagaccttgc gacagcgtat acggtgccac aaaacctgaa
    27061 gaatgcactt tcgttgcgtc aaactgtgtt gttgcgcaga attttttcac gcgcacagag
    27121 ctgacagcgt attttttgcc cgtaattttg ctcaggatat caaagcaata tccacccagc
    27181 attcctagtg ggtaaggcaa gtgcatagaa gggatctttt tgttcaggct ttgttcaact
    27241 tcagcaacca actggttcat gttcaggtct ggcttatcaa catagttata aacctcataa
    27301 cctgcggcaa cattcttcag tttgtacttg ataaactcaa caatgtttcc aacataagcc
    27361 atggacttat agttagtccc tgcgcccacc atcataaact tgccgccagc gatctgtttc
    27421 agcaagttat agacgttacc gcggttgcgt tcaccgaaga taacggtagg acggatgatg
    27481 gttaatgaac gttctgttgg tgctttgtta taccattcac gcagcacttc ctctgcctgc
    27541 cacttacttt tgccgtagtg gttgaaaggg tcgtgtggat ggttttcgtc agggttgtgt
    27601 ttgttcaaac cataaacagc aacggaactg gtaaagatga tatttttaac gccatttttt
    27661 tccatggccg ccagcacatt gcgggtaccc tgaacgttga catcataata gagagaagta
    27721 gggctgacgt catcgcggtg ttccgctgcc agtagtacaa cagtgtcaaa accggctaac
    27781 gcctggtcga gtgcctgttg atcacgaaca tcaccaatct gtgtgatttc tggataaaag
    27841 tggctctgcc gtttgtccag gttcttgata ttaaagtcag caattgccgt ttcaagtagt
    27901 cgggttccta cgaatccgga agctcctatg agcaaaacgt tattgttcat aaatcacttt
    27961 agtctggttg ttacgtaaga aacacaagat aaagatgagt accttccctg agtagtcaat
    28021 gctgcccagc cccagcttta acagttagtg tgaggattat aatcttttag aacattatat
    28081 ccagtaagtt tatgaatggt cgcaaatcta ctctctccgt tccggcaatc taaagttaat
    28141 cttttccaca ttaacaatat ggtgattaat cctgtcgatg tcgacggagc tttgccctct
    28201 ttcattcatc gcatgaacat ttgcaagtga cagcagtgtt tcttttttcg ccataaagac
    28261 accgcgtacg tctttgcgca tgtcgaagtt catgctcaat gctggtccaa ctgaggattc
    28321 ctgcatcacc ttgatattac gcataaaaag atgttgcggt ttgttgtgta actccagcga
    28381 ggcacgcttc atctcaatgt tagtcagtgc cacaaaggag acagcattcc cggcggagat
    28441 ttggatgccg cgcaatttat aaacaagatg ggtgttatcc agttgaatat tattcactcg
    28501 gaaattttgc ggtatcgaga gatatttgcc tttaattacc ccatagccga ttaacatccc
    28561 ggcgctatta atcatttcaa tattatcaat cacgaaattg tcacaaccgt aaatagcaac
    28621 tgtcgcgtta tcaatgcctg ctttcttact gaaatccggc gtgatattgc gggctttgat
    28681 attacggata acaaaatgtt tgccattttc aacatggatc aactgccgac aatccgatcc
    28741 cgtgatattc gccacgacaa agtttttcac tgcctggtct tccgggtagt tattatcgta
    28801 ggtactcccc gcaaggccta tgccaattcc ccagttgatt ttgccattag tacagttgat
    28861 gcgctcgatg acatggtcgg agatcaaaat atcactgtcg ttaattgcca cgttccattc
    28921 aatggcgtcg ccctgtaagt cgctgaactt acaattggtg atgttggcac cgataatctg
    28981 gttatgaaat ccctggcgta agatggcgta attagcgtgg ctgacagtga ggttatcgat
    29041 ggtcaggttg ctcatgaccc gtttgttttt gccgccgata taaatctgcg ttaccgggcc
    29101 aaagccgctc atcgccagcc ctttgatggt gcagtcagaa ccacgcacat ccaaggtgat
    29161 gttatgcata ctgccgccct cctcccctgt cacctggctg ccgtcctgta agacaaatcg
    29221 ccctctgccg ttgccgcgca agcttccaag aatgtgtaac gttttaccgg gagggataaa
    29281 gatgccggtg ttgatattgt cacaaaccaa tccggcaggc acgacgaccg tatgcccttc
    29341 gctgaaggct tgtttaaatg aggcgatcca gtcgtgtggg ttgtagtcgt taatgttaac
    29401 gctttgtcgg gcgggaagtg cgcgagcgaa aggggtatgg aggaaggcaa gcgccgagct
    29461 tgccgtcagg aaggtgcgtc gggagagtgt tttaaatggc atgcgttctc ctctataaag
    29521 cttgcagcaa gctggcgagt tctcgattga tcacctgctg gttaaaatcg tgttcgactt
    29581 tttcgcgcgc acgtttgaca accggagcca gttcgtcggt gcccagttgg ctaaacgccg
    29641 ccagtcgttg cgccagtgcg cgagcatcgt tctcaggcac cagccagccg gatttgtcag
    29701 cctccaccag ttccggtatt ccgctatgca gagtagaaac caccggaata ccgaccgcca
    29761 tcgcttccat tagcgccacc ggaatgcctt ccatatcacc atccgcgccc gttaccgatg
    29821 gcaacagaaa gacatccgcg tcgtcgagca tcgctttcac ttcgtggctc ggtttaaagc
    29881 ccggcatctc taccacatct tccagttgat attgttcgat gagggtacgc aggcgtcgtt
    29941 cccacgggcc aatgccgagg atgcgatagc gaaatgccac gccctgctct ttcaactgac
    30001 ggcaggcttc aatcgccaca tgcaggcctt ttttctcggt taagcgtgcg acggaaatga
    30061 tttccagcgg cgttgcgggc gctttcaccg gacgcgggct aaagcgcgtc atgtccacgc
    30121 ccatacgcga tacggcgatt ttttccctcg ggcagcccat tttttgcagc cttccggccc
    30181 acaaatcgct tatcggtaac atcaggtcgc cacggcgaaa cagttgttga tattcgggag
    30241 tgtaatggtt gagcacttcc cgactggaaa tatcaatgcc gtggaaaatg gtggcaattt
    30301 tgccgcgaat gacacccagt tcccgtagtt ttgctgcggt tacccccgcc gggccaaaat
    30361 gagcgatgaa cacatcggca tgaagcggtg ttgcgacctg gccgcaaatg gcagacaaaa
    30421 tcaggttccg cgactcggca ccatagcgtt tgaggttgag cgcctgccag gtatttttac
    30481 gataaatgcc gcgtaacgtc tggctggcgc ggtggcgcag tttcgctatt ttgcccgtag
    30541 gttcgtcctg taaccagtgg gttctggcgg caaggttgta tttcgtccat gccgcgtggg
    30601 tgttttgtgt gtcgcctttt tgcagcgcga gaatctctac ctcaaatccc atatcaataa
    30661 acgcggtaat ttggttaagg acgaaggttt ctgacgacag cgggaatttc agtaaaaaga
    30721 agccgacctt catttcacct ccccgatgcg ctcaagcaca gactgcacca tctgcattcc
    30781 tgtctgacgc tcacgactga cggcttcact aagtcgcgca ttcagtgccg gaagctggcc
    30841 taaggtatcc gcaaccatcg cttgcaggct gccgtctaat aaatgacgga tatcaattgc
    30901 catctccggt agtcccagct gctgcataat cccggcggat ttatgttcgt agttgatggc
    30961 aattgccgga gtggcaaaat tcatcgagat aatggcagag tgcaggcgcg taccgacggt
    31021 gagttcacag gcaccgagaa ttttgcccat ttccagatcg ttgagttcat ccatcaccac
    31081 atggtaacgg gcaggatcgc tgatgtgctg gcgaagattg agcgccatca tgcggtcgtc
    31141 tttgttatag ctgtcgatac ccgtacaggt ggagagtgca atgacctgac agccttcgtc
    31201 gagaatgcga ttgaccaccc cggcaaaggc tttttcatac gcttgttgag tggtgccgag
    31261 gcgtttgtca aacggtgcca gttcgcgcag ggtaattgct accgttttct gttgtgcggc
    31321 aacgtccagc cagtgttgaa cagcatagct ggcggtgaag tcttcggtgt ggtgatcgac
    31381 cagccacgcg gtatcgacgc catgttccac ttttgcggta gtgatattgc tgcgtttcat
    31441 cagatcaaga ctgaccgatt cgcgcaggat cagcgcgtca cagtgaccaa aaacgtagtt
    31501 cgccagttgg ttaaattgct catcctggaa ggggccgacg ctgtgaccaa tcataaacag
    31561 cggctttttc gccataaacg tgcaaagcgc atgttcaaac tgcggcacgc cgtagagatc
    31621 gacaaaaaac gatcctccga cctgaataat ggcgtcgtaa cctgagagca ggcgcacgaa
    31681 gtcggtgaat ccctgggcga tggcgatatt acgcagcttg ccagtgtcgg tgacgcgtga
    31741 gagcaatacc tggtgttgat agcggcgacg gaggactttt ttaacgcgcc cgacaacgcc
    31801 cgccgcgctg ttgtgttgtt tcatttgcag gaacagagga tcgcccatta ccgggcggtt
    31861 gagtagccag gaagaactga ccggatagcg gctcatcacg tccacttcgg cttgtggatt
    31921 gaggatgttg atggcatcaa gtaagccgcg caagatggcg ctgtcgccac gattgccgca
    31981 agtgtggttg cccagaataa gtaatttcat tttttcctca taaatttgat gccaagtgag
    32041 gctgtgtttg ttttgttcgt tgccgctttt tgcctgatgc gacgctgacg cgtcttatca
    32101 ggcctacaag acccgagcac agaaccgtag gccggataag gcgttcacgc cgcatccggc
    32161 attcagtgcc tgatgcgacg ctgacgcgtc ttatcaggcc tacaggtccc gagcacagaa
    32221 ccgtaggatg gataaggcgt tttacgccgc atccggcaac cgttgtcgga accgaaaaca
    32281 gcaactcacc ccgcccgtaa aagcattttc attttttcgc tgcgacaaaa ctgacgcttc
    32341 acttccacca ccagcggatg gcgcgacagc acaatcatca ccacaaacgc cagcacgccc
    32401 gcggctattt gcaccgccag cagcatcccc agcgccagtt gccctttcag cacaatgccc
    32461 agcgcataac tgaccaccag cgtcggcaac gagagataaa acggcagcca caaactcagg
    32521 atgtactggc gataactgga accaagaacc ggtttaatca tcacgaagta actcagaatg
    32581 gtgttgataa tctgcaccag caggaaaccg agcgtgacgc cgattgcgcc cgccatctgc
    32641 ccaccaataa caatcgccgg aataaacaga aatgttttga atacgttgaa tttaaagctg
    32701 atatcgaccc gcgctttcgc catcagcagc gaaccaatcg ggttacccac ggagcgcagt
    32761 agccccacca cacacagaaa ttgcagcacc ggaataatgc tgttccactt ctcaccaaag
    32821 accagtggta caaagttatt cgacaccacc attagcccga gcagcgccgg aaagttgata
    32881 atccccacca ccgacagcgg tttgtagaag ttaatacgca gcttttcggt gtcgtcctga
    32941 attttggcaa acgccggaaa caacacacgg gtgatgattg ggttcagctt catcggcggc
    33001 acaacggcca cgttgtaagc caggttgtag ccccctgcca cgcccgcgcc gagaatacgc
    33061 gccagcacca gcgttgaaag gttggtattg agatagttga tgatgctgtc cgccgtcagc
    33121 caggcaccaa agcgtaagtt cggtgccacc gacgccagcg agaaatgcag accggggcga
    33181 taaattttgc ggccaaagta gccaaacagc agcgttctca ccgcactatt gaccagataa
    33241 ccgaggatcg cggtcatcgc cagcggccag aaatgggcgc taaccaccgt aaaagtgaag
    33301 cccgccagta ccgcgctggt ttcgatcatg ccgattttgt tgaactccag ctccttttgc
    33361 atcaacgcgc ggaactgctg cccctggggg atcaccacaa acgccagcga caatgtttta
    33421 atcaacggtg ccaggtccgg gttattcagc acgtcgccga tgagatcact caacaaaaac
    33481 accgccagac acaccacaag ccccagcccg acgttcagcc agtacagcgt ggtgagttca
    33541 aggtggctga tttcttttcg ctgaataatc gagttagcga taccgaagtc agaaagcgta
    33601 tctgccagcg cgatgatcac cagcgacaca gtaagcaaac cgaactggtg gttgtcgata
    33661 atccgcgcca gcacggtcat ctgcaccagc ccgaggccga tgatgatcac cgtggcaatc
    33721 gccgaccact tcgcgccgct gatggttttt tcacgtaagc tcatatcaat atgccgcttt
    33781 gttaacgaaa cctttgaaca ccgtcaggaa aacgattttg atatcgaacc agacgctcca
    33841 ttcacggatg tactcaaggt cgaactcgac gcgtttttcc attttctcca gcgtgtcggt
    33901 ttcgccgcgc cagccgttaa tctgcgccca gccggtaatg cacggtttca ctttatggcg
    33961 cagcatgtag ccttcaatga gctggcgata ctgttcgtta tgcgctactg cgtgcggacg
    34021 tggaccgaca atcgacatcc ccccggtcag cacattgata aactgcggta attcatccag
    34081 cgaggtgcgg cgcagaaagt tccccacttt agtgacgcgc ggatcgttct gcgtcgcctg
    34141 ggtcaccact ttgtcgttct ccatcacttt catggaacgg aacttccaca ctttgatcgg
    34201 cttgccatcc atgccgtagc gagtctggcg gaaaataacc ggccctggtg aactgagttt
    34261 caccgccagc gcaatacagc acagcaccgg ggagatcagc agcaagatca gcgtcgccag
    34321 cacaatgtct tccgcacgtt ttagcaggcg gttaaccccg gaaagcggcg tgtcatacag
    34381 cggcaccacc ggaacgccgt tcatctcttc gagacgtgaa tggagaatat tgaaggtaaa
    34441 gacatcgggg atcagcagca ccgaacaggt ggtgtccgcc agttgatgga ccagtttttt
    34501 cactcgcgcg ccgtcgcaca tttgcatcgc gatatagacg ttatgaatct tacccgcttt
    34561 agcatcctcg accagctgtt gcagattgcc cgcccagtcg ttagaaacac cgcccggttt
    34621 cgggtcgtgg taaacgccca ccacttcaaa ccctaaccac ggctgattac ggaagctctc
    34681 catcagcatt tgtccggcgg ctaaatcccc cgccaccgcg accatgcgct tgttatagcc
    34741 atgattacgc agccagcccg ccccaatgcg aatacatgaa cggcaaacca ccagtccgat
    34801 actggtcagc ccataccacg ccagccagat tttcagttgc gtgtcgaaat cattgttgaa
    34861 cgccaccagt ccggcgctga aaatcacgct taaggtccag ttttgtagca acagggcaaa
    34921 ttctgtcgct gcccgaacac cgcgccatga gcgataaaaa tcggtgatgc cgcccagcat
    34981 ctggaacacc accagcgtaa tcaacgccac caacaggtgc atgtagagga atgacagtcc
    35041 gctcacttcg caaaccagcc atagtccggc aaacatgatg gtgatatctg aaaagcgttg
    35101 caccatagag attaacgatg cattggtttt cgctcgcgct tttttagatt tgtcatcgtt
    35161 gttcctgtta ttagcccctt acccgcagtg gggtaaggga agatccgaca ttactcgttc
    35221 agcaacgtca gcagagttcg cgttcgcgct tccatcagcg gcacatcacc gcgtgattcc
    35281 acattcaacc gcaccaccgg ttcggtgttg gaggagcgca gattaaagcg ccagtcggca
    35341 aaggtcatgc tgatgccatc ggtgcgatcc accgccagcg cctcacggct aaaatgctgt
    35401 tccacgcggt taatcgcctc aacgggttgc gccagtttgc tgttgatctc accgtttgcc
    35461 ggaaacgccg ccatccggtc gcgcaccagt tcgcccagcg tttttccttt caggcacacc
    35521 agttcggcga ccagcagcca cgggatcatg ccgctgtcgc agtaagcgaa atcacggaag
    35581 taatggtggg cgctcatttc gccaccgtag atggcgtctt ccttgcgcat acgttcttta
    35641 ataaaggcgt gtccggtttt cgacatcacc gacgtgccgc cagcggcagt caccacatca
    35701 acggtattcc aggagagacg tggatcgtgg atgatcttcg cgccgggatt tttttcgagg
    35761 aacgcttctg ccagcaggcc gacaatgtag tagccttcga taaactgccc tttttcgtca
    35821 aacaggaagc agcggtcaaa atcgccatca aaggcaatgc ccatatccgc gccgtgtttg
    35881 atgaccgcat tgcgggtgtc gtcgcggcat tccggcagta gcgggttagg aataccgttg
    35941 gggaaattgc cgtccggcgt gttgtgcact ttgattaatt ccacgggtgc gccaagggct
    36001 ttaaagcggg cttcaatggc gtccaccacc ggacccgctg cgccattccc ggagttaatc
    36061 accagcttga gcggcgtgag gtttttgaca ttgatataac cgaacaggtg atcaacgtaa
    36121 gcgtcacgca ggttgatttg ctgatagcga ccgcgtttgg tttcatcgac gggaggaaag
    36181 tcgttggctt cagccagacg ctggacgtcg cgcagtccgg tatcaccgct gatcgggcga
    36241 gccccttcgc gcaccagctt catgccgtta taatccatcg gattatggct ggcggtgact
    36301 tcaatgccgc catccacgcc gagatggaac gtggcgaaat agatctcttc ggtgccggac
    36361 ataccgatat ccagcacgtc aacgcccgca tcctgtaaac cttttgccag cgccagtttt
    36421 aaggtttcgc tggtgagacg gacatcaccg cctaacacaa tggttttcgg tttgagaaat
    36481 tcgccatagg cgcgaccaat gcgccaggcg atatcttcat tcagttcttc gcctaatttc
    36541 ccgcgaatat cataggcttt aaagcaggtt aattttttca tgtcgttacc ctttttcagg
    36601 caatagttgg ccctgaccga aacggccaat tttgtttttg tcgttattta catctcggtt
    36661 tcgcattcat tgcctgatgc gacgctgccg cgtcttatca ggactacaaa cccgaaccgt
    36721 aggtcggata aggcgttcac gccgcatccg acaaacagcg cctgatgcga cgtttacacc
    36781 cgtccgtagc gatccgcgaa gcgcaccaca tcatcctctt cgagataaga gccggagcgc
    36841 acttcaatta aatcaagcgg aattttcccc ggattttcca ggcagtgcgt cgcccccagc
    36901 ggaatataaa tggactcgtt ttcaccaagc agtttgatat caccatcaat ggtgactttt
    36961 gccgttcccg cgaccactac ccagtgttcc gcgcggtgat ggtgcatctg taccgacaaa
    37021 ccctcgcccg gtttcacggt gatgcgtttc acctggtagc ggtcgcccgc gtcgatagag
    37081 tcatatttgc cccacggacg atacacttcg cgatgcaccc gatgctcatg gcgaccatcg
    37141 gctttgatct gctcgaccac ttttttcaca tcctgtaccg cgttacggtc ggcaatcagc
    37201 accgcatctt tggtctgcac cactaccaga tctttcaccc cgacggtggt gaccaggcca
    37261 gattcggcat acacatagct gttttcagtt ttgtgattaa tcacatcgcc gtggcaaacg
    37321 ttgccctcgg cggtgtgggc gctgatctcc cataatgaag accaggaacc aacatcgctc
    37381 cagcccgcat ccatcggcac cacaacagca tctgccgtac gttccatgac cgcgtaatcc
    37441 accgactctt ccgggcaggc gagaaacgct tcttcatcca cacgaataaa atcaagatcc
    37501 ggatcgacgg agctcatcgc tttttcacag gcatcgagaa tatccgggcg atattttttc
    37561 agttcttcga gatagcgtcc ggcgcggaac aggaacatgc cgctgttcca gtaatattcg
    37621 ccgcttgcca cataggccta ggcggtttcc agattcggtt tttcgacaaa ctgcgccact
    37681 tcaaaggcca ccgtatcctg ctcacccgca gacacttcac cgcgacgaat atagccataa
    37741 ccggtttctg gtagatccgg cacaatgcca aaggtcacca gcttgcccgc ttcggcatat
    37801 ggcatggcat tacgcacggc ggcacggaac gcgtcttcat cggcaattac atgatccgcc
    37861 gccagcacca gcattaacgg gtcgttctcc gggctatgac gttttgccgc cagcgccgcc
    37921 agcgcaatgg ccggtgcagt gttacgccct gcaggttcga gaataatgtt ctcggtgagt
    37981 ttgttcagtt gacgcagctg ttccgcgaca ataaagcggt gctgctcatt gcaaatcacc
    38041 accgggcttt cgcactccac gccgttcagg cggcagatgg tggtttgcag catggtgaga
    38101 tcgcctttca ggcataaaaa ctgcttgggg taaagtacgc gggaaagcgg ccataagcgg
    38161 ctaccggagc cacctgccat cacaactgga tagagtttcg actgcgccat aattatcccc
    38221 gaatatcatt tataaattga cgtaacacgt tctctttatc gagcgtgcgt tcggcatatt
    38281 cacgtgccac cgtgttgtgt ttgggcagca ggagcgcctg actaatcccc gccaccagcg
    38341 cctcgaccga ttccggctct acgcaaaccg caatgcccgg aaaggtttcg caaagctgcc
    38401 ccagttctgt gtgggcttct gcggtaatca ccgcgttacc gcctaccgcc agaatattgg
    38461 tcagtttcga cggcaatacg gcatctgccg cgccgcattt ttgcaccacc agatggcaat
    38521 cgcccatctt cagcagtgcg ggtaaagcgt catacgattg cagcgggaaa aattgcatgt
    38581 tgcgcagtcc acgctgctgc gccatttttt ccagccgcgc tttgccgccg ccctgcccga
    38641 caatggcaaa aatcagcggt tcatcgcgca ggcgatcggc agcttcaata acgttttcca
    38701 gtccctgctt ttcaccaata ttgccggagt aaagaatgat ttttttgtta tccggtaggc
    38761 caagctggtt acgaagggca tcaacatcgg cgtctgcaac atgctgaaaa cgggcgattt
    38821 ccgaccagtt ggggaagaaa atgatgttat ccgccgccac gcctttttcg atggctttat
    38881 tcatcatcga acgcgaaatc gtggagacgt tatcgacgtt atgcagtccg ctacgttcga
    38941 acgccgttgc cagctgtgcc actttgccgc ctttgccttt tccggcaagg cccagcccca
    39001 gcatggcgtc cacttcgtaa tcctgaatat gcagcacggt acgcgcacca gagagtttcg
    39061 ccagcaggcg cattcccggc gtgcaaaaca gcgttggcac cacgccgata atgcgatccg
    39121 gcttccagcg acgttgcgcc atcaacggga aaaaactgct gacggcaaaa ccgccgagat
    39181 gcaacaggcg tttcagggtg ctcggctgtt ttggcacgta cagcgggcag cgccacaccg
    39241 tggcggcccc ctcttctcgt ttgtagcgct aggcggaata gttctcgccc acctgccact
    39301 gcgggtagta aggcggtgcg gtaataaccc gcacctcatg accttgcgcc gccagccatt
    39361 ccaccatttc tccggtgtat ttgccgatgc cggttaactc cggcgagtag ttaatgccgt
    39421 agaccagtat tttcataatc cgggtactcc ggcacgcttc tcagcgagaa aataggcgcg
    39481 gctgttagcg tgaacattgt cactggcgag cagtgcgtcc ggcgtcagcc agcggtaatc
    39541 gtcatgctgc tcatccggca gtaacagttc ttcttccgct actctgaagc gaaaaccgag
    39601 caccacatag tgagtggtga aatccgtgcc agagaagtta tcgtcataaa agtgctgcca
    39661 gacaccgtaa aactggcctg ctgttatcgg cagacgcagc cccagttccg ccatcgtcag
    39721 ccgctcaaat gcggcttcca gcgtttcgtc tttctgcacg cgccctcccg gcacaaacca
    39781 gtaaccctgc gccgggcggt tggttctttt gccaggcaga aactcgccgc gactattctc
    39841 gacaataaag tcgagagaga caagcggtgt ggagcgcact accgtggcaa agtcttcctg
    39901 acgtaaaaac atcattaccc ccgaaagcgg tcttgattct caaggaacca ctggtaagtg
    39961 ctggcaagcc ccgcttccag tgagatttcg tgataccagc caagctgatg caggcgcgtc
    40021 acatcaagca gtttgcgcgg cgtaccatcc ggtttactgg catcaaaaac cacccgacct
    40081 ttgtaaccca ccactttggc gatggtttgc gccagctcgc ggatagtgca gtcaacgccc
    40141 gtgccgacgt taatgtgcga cagcatcggc tgggtgtttt ccagccagac ttcgtgcgcc
    40201 agctccatca catgaatgct cgccgccgcc atatcatcga cgtgcaggaa ttcacgcatc
    40261 ggtgtaccgc tgccccacac caccacgtcc ggcgcattct gtgccgtcgc ctcgtggaag
    40321 cgacgcagca atgctgggat cacatgcgaa ttactcgggt ggaagttgtc atgcgggcca
    40381 tacaggttgg tcggcatgac cgagcggtaa tctcgtccgt actggcgatt gtaagattcg
    40441 cacagtttga tcccggcgat tttggcaatg gcgtaaggct cgttggtcgg ctccagcgtg
    40501 ctctgcaata actcgctttc tgccatcggc tgttttgcca gtttcgggta gatgcaggac
    40561 gatccgagaa acagcagttt gttcacgtcg ttctgatgcg cggcgtgaat gatgttgctc
    40621 tcaatcatca tgttctggta gatgaaatcc gccggatagg tgttgttggc aacaatgccg
    40681 cccactttcg ccgccgccag atagacctgg tcaatgcgtt cgctggcaaa gaaatcatgc
    40741 acggcgcggc tgtccagcag gttcagctcg tcgcgggtac gtaataccag ctccacatca
    40801 ccgcgctgtt cgagctgccg cttgatggca gaaccgacca tcccacgatg gccagcgata
    40861 aaaatgcgtt gtttactcat gcttatgact ccagcgcgat cgccacgtcg tagccgtgag
    40921 atttcagcag agagtgtttt ttcgccgctt cgaggtcatt agccaccatt tcagacacca
    40981 tctctctgag ggtgatttcc ggtttccagc ccagtttttc gtgcgctttg gtcgggtcgt
    41041 cgagcagcgt ttcaacttct gccggacgga agtaacgcgg atcaacggcg ataatcacat
    41101 cacccggttt aacgcccggc gcgtcatgcc cggtgacgga aaccacaatg cccttctctt
    41161 caacacccgt gccttcaaag cgcagtttga tgcccagctg tgccgctgcc atttccacga
    41221 actgacgtac ggagtactga acgccggtca cgataacgaa atcttccggc tgttcctgtt
    41281 gcagcatcat ccactgcatt tttacgtagt ctttggcgtg gccccagtca cgcagggaat
    41341 ccatattgcc gaggtacagg cacgactcca gcccctgggc gatgttggcg attgcgcggg
    41401 tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga ttcatggttg aagagaatac
    41461 cgttacaagc gtacatgccg taggattcgc agtagttaac ggtgatccag taggcgtaca
    41521 gtttggcgac cgcatacgga gatcgcgggt agaacggcgt ggtttctttc tgcggaattt
    41581 cctgcaccag accatacagt tcagaggtgg aagcctgata gaaacgagtt ttcttttcca
    41641 gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc catcgcgtcg acgtcagcgg
    41701 tatattctgg tgactcaaaa gagaccgcaa cgtggctcat tgcgcccagg ttgtacactt
    41761 catccggctg tacttcacgc aaaatgcgcg tcaggttaga ggtatcactc aggtcgccat
    41821 aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg ataaatgtga tccacgcgct
    41881 cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac ctcgtaacct ttttccagca
    41941 gaaactctgc caggtaagaa ccgtcttgtc cggttacacc ggtgatgaga gcgacttttg
    42001 acatgtctta ttcctctgta tttttgaatt tattcagttt caacgcgttc gcgtatcacc
    42061 actgcgggat tcccccggca aaccacattt gccggaagcg atttaaaaac actgcttcgt
    42121 gcacccacga cggtgccgtc gccgattgtg acgcctgggg caacaaagac atcggttgcc
    42181 agccagcatt tctcgccaat cacaataggc gtggcgttaa tggtgaaatg ttgacttgca
    42241 tggtcgtggc taccggtgca taaataactt ttttgcgata tcaccgaatg tgcgccaatg
    42301 gttatttcac cgagggtata taaattgacg tcatcgccga cccacgcgta atcacctaag
    42361 gttaatttcc acggataggt
//
LOCUS       DADLTD010000006        42356 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLTD010000006 DADLTD010000000
VERSION     DADLTD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.006"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="JN701_000246"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="JN701_000246"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY7362794.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(1711..3324)
                     /locus_tag="JN701_000247"
     CDS             complement(1711..3324)
                     /locus_tag="JN701_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7362795.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPP"
     gene            complement(3783..3869)
                     /locus_tag="JN701_000248"
     tRNA            complement(3783..3869)
                     /locus_tag="JN701_000248"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3833..3835),aa:Leu,seq:gag)
     gene            complement(3884..4216)
                     /gene="secG"
                     /locus_tag="JN701_000249"
     CDS             complement(3884..4216)
                     /gene="secG"
                     /locus_tag="JN701_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY7362796.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4444..5781)
                     /gene="glmM"
                     /locus_tag="JN701_000250"
     CDS             complement(4444..5781)
                     /gene="glmM"
                     /locus_tag="JN701_000250"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY7362797.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELGLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5774..6622)
                     /gene="folP"
                     /locus_tag="JN701_000251"
     CDS             complement(5774..6622)
                     /gene="folP"
                     /locus_tag="JN701_000251"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY7362798.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6712..8646)
                     /gene="ftsH"
                     /locus_tag="JN701_000252"
     CDS             complement(6712..8646)
                     /gene="ftsH"
                     /locus_tag="JN701_000252"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY7362799.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8746..9375)
                     /gene="rlmE"
                     /locus_tag="JN701_000253"
     CDS             complement(8746..9375)
                     /gene="rlmE"
                     /locus_tag="JN701_000253"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY7362800.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9501..9794
                     /gene="yhbY"
                     /locus_tag="JN701_000254"
     CDS             9501..9794
                     /gene="yhbY"
                     /locus_tag="JN701_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY7362801.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9951..10427)
                     /gene="greA"
                     /locus_tag="JN701_000255"
     CDS             complement(9951..10427)
                     /gene="greA"
                     /locus_tag="JN701_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7362802.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10675..12108
                     /gene="dacB"
                     /locus_tag="JN701_000256"
     CDS             10675..12108
                     /gene="dacB"
                     /locus_tag="JN701_000256"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7362803.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12148..13320)
                     /gene="cgtA"
                     /locus_tag="JN701_000257"
     CDS             complement(12148..13320)
                     /gene="cgtA"
                     /locus_tag="JN701_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY7362804.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13336..14301)
                     /locus_tag="JN701_000258"
     CDS             complement(13336..14301)
                     /locus_tag="JN701_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7362805.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14428..14685)
                     /gene="rpmA"
                     /locus_tag="JN701_000259"
     CDS             complement(14428..14685)
                     /gene="rpmA"
                     /locus_tag="JN701_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY7362806.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14706..15017)
                     /gene="rplU"
                     /locus_tag="JN701_000260"
     CDS             complement(14706..15017)
                     /gene="rplU"
                     /locus_tag="JN701_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY7362807.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15276..16247
                     /gene="ispB"
                     /locus_tag="JN701_000261"
     CDS             15276..16247
                     /gene="ispB"
                     /locus_tag="JN701_000261"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY7362808.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16475..16753
                     /gene="sfsB"
                     /locus_tag="JN701_000262"
     CDS             16475..16753
                     /gene="sfsB"
                     /locus_tag="JN701_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY7362809.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16801..18060)
                     /gene="murA"
                     /locus_tag="JN701_000263"
     CDS             complement(16801..18060)
                     /gene="murA"
                     /locus_tag="JN701_000263"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY7362810.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(18115..18369)
                     /gene="ibaG"
                     /locus_tag="JN701_000264"
     CDS             complement(18115..18369)
                     /gene="ibaG"
                     /locus_tag="JN701_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY7362811.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18529..18822)
                     /gene="mlaB"
                     /locus_tag="JN701_000265"
     CDS             complement(18529..18822)
                     /gene="mlaB"
                     /locus_tag="JN701_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY7362812.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18822..19457)
                     /gene="mlaC"
                     /locus_tag="JN701_000266"
     CDS             complement(18822..19457)
                     /gene="mlaC"
                     /locus_tag="JN701_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY7362813.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19476..20027)
                     /gene="mlaD"
                     /locus_tag="JN701_000267"
     CDS             complement(19476..20027)
                     /gene="mlaD"
                     /locus_tag="JN701_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY7362814.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(20032..20814)
                     /gene="mlaE"
                     /locus_tag="JN701_000268"
     CDS             complement(20032..20814)
                     /gene="mlaE"
                     /locus_tag="JN701_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY7362815.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20822..21631)
                     /gene="mlaF"
                     /locus_tag="JN701_000269"
     CDS             complement(20822..21631)
                     /gene="mlaF"
                     /locus_tag="JN701_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY7362816.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21841..22818
                     /locus_tag="JN701_000270"
     CDS             21841..22818
                     /locus_tag="JN701_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY7362817.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22832..23818
                     /gene="kdsD"
                     /locus_tag="JN701_000271"
     CDS             22832..23818
                     /gene="kdsD"
                     /locus_tag="JN701_000271"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY7362818.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23839..24405
                     /gene="kdsC"
                     /locus_tag="JN701_000272"
     CDS             23839..24405
                     /gene="kdsC"
                     /locus_tag="JN701_000272"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY7362819.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24402..24977
                     /gene="lptC"
                     /locus_tag="JN701_000273"
     CDS             24402..24977
                     /gene="lptC"
                     /locus_tag="JN701_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY7362820.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24946..25503
                     /gene="lptA"
                     /locus_tag="JN701_000274"
     CDS             24946..25503
                     /gene="lptA"
                     /locus_tag="JN701_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY7362821.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25510..26235
                     /gene="lptB"
                     /locus_tag="JN701_000275"
     CDS             25510..26235
                     /gene="lptB"
                     /locus_tag="JN701_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY7362822.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26283..27716
                     /gene="rpoN"
                     /locus_tag="JN701_000276"
     CDS             26283..27716
                     /gene="rpoN"
                     /locus_tag="JN701_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY7362823.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27739..28026
                     /gene="hpf"
                     /locus_tag="JN701_000277"
     CDS             27739..28026
                     /gene="hpf"
                     /locus_tag="JN701_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY7362824.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28144..28635
                     /gene="ptsN"
                     /locus_tag="JN701_000278"
     CDS             28144..28635
                     /gene="ptsN"
                     /locus_tag="JN701_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY7362825.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28681..29535
                     /gene="rapZ"
                     /locus_tag="JN701_000279"
     CDS             28681..29535
                     /gene="rapZ"
                     /locus_tag="JN701_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY7362826.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29532..29804
                     /gene="npr"
                     /locus_tag="JN701_000280"
     CDS             29532..29804
                     /gene="npr"
                     /locus_tag="JN701_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY7362827.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            30018..30650
                     /gene="yrbL"
                     /locus_tag="JN701_000281"
     CDS             30018..30650
                     /gene="yrbL"
                     /locus_tag="JN701_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY7362828.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30647..31375)
                     /gene="mtgA"
                     /locus_tag="JN701_000282"
     CDS             complement(30647..31375)
                     /gene="mtgA"
                     /locus_tag="JN701_000282"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY7362829.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31372..32025)
                     /gene="elbB"
                     /locus_tag="JN701_000283"
     CDS             complement(31372..32025)
                     /gene="elbB"
                     /locus_tag="JN701_000283"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY7362830.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32255..34591)
                     /gene="arcB"
                     /locus_tag="JN701_000284"
     CDS             complement(32255..34591)
                     /gene="arcB"
                     /locus_tag="JN701_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY7362831.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34687..35616)
                     /locus_tag="JN701_000285"
     CDS             complement(34687..35616)
                     /locus_tag="JN701_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY7362832.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36291..40751
                     /gene="gltB"
                     /locus_tag="JN701_000286"
     CDS             36291..40751
                     /gene="gltB"
                     /locus_tag="JN701_000286"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY7362833.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKAFYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40764..42182
                     /locus_tag="JN701_000287"
     CDS             40764..42182
                     /locus_tag="JN701_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7362834.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
BASE COUNT        10534 a        11368 c        10441 g        10013 t
ORIGIN      
        1 ctgaaactcg gcaaattcta ctcgttttgg gtaaaagatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 gccttctcct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tctggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 aggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
     1021 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ctgaacggta aaacctttag caacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc gtatcatcga
     1201 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
     1261 acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctactgt accaggggcg ttggtttgac tcccaggcgc tgatgctgcg
     1381 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
     1441 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta ttggtcaatt
     1561 gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt atgccaaaac
     1621 tggcctgctc tcctcctctg ccacttcagg cgtgccgcag gtggagaata tggaaaacaa
     1681 aggccagtaa ttcgacatca accctgccct tcagggggga gggttacctt gcttatcctc
     1741 tgtaaactcc tttctgaaca tcacataatc gaaaacatca tggtttgcat ccacaacccg
     1801 tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt
     1861 tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga tgttgttttc
     1921 cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca tcgggacatg
     1981 atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct tcgtagggtc
     2041 acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt ttaataattc
     2101 aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc aggcatcctg
     2161 atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag ccggttcatg
     2221 gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct gtaatgcttg
     2281 cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt ccatggcgcg
     2341 catggcgata ctggtaactg cagtaccatt ctgccgaaaa gcggattgcg agcttagcca
     2401 gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt aattatgaat
     2461 gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg cagtgtaagg
     2521 tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt caacttgcgg
     2581 tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag actcccccac
     2641 aatcaacacg taggtatcaa tacctgtatc cctgactgat aattgaaaat acggcaccgt
     2701 gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat agttgagatt
     2761 gaaaaacggc gtataggtag caaatcgcga cgctagtata tatggactga acgttttttt
     2821 attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg aaatgacaat
     2881 cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt ttattattac
     2941 tgcccaaaaa agaagggata aaaaagcaaa ggcacataga taagggatat acatccccag
     3001 cattttgaca acttcatccg gatcactctg gagcacacta atcgcaaatc catcattgaa
     3061 agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg cagtcagtaa
     3121 cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt ttacaaccag
     3181 gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gcgccggaag aggcgactaa
     3241 gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt taaaacttct
     3301 agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata taaacaatta
     3361 tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata tcagaccgaa
     3421 acactgttag ttttttcttt ttaaatgatg cggccattgc gttctcagta attaaataac
     3481 attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg
     3541 agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca
     3601 caataaagtg ccaattatgt cagcagaagg gaaaagttta taactaaggc gtaccggcac
     3661 catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa
     3721 ccattcctca gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggag
     3781 tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt
     3841 agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc
     3901 tggtcggctt agccggagca gctggttgag tttgttcggt tttcgccggt gcactcagat
     3961 tttcccattc gctaccttta ttggttttgt tgctgttgat gttacccagc accagactga
     4021 tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta ccagaaccac
     4081 ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt
     4141 taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacca
     4201 aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa
     4261 ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc
     4321 gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa
     4381 accgatgtgt tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca gtcatttatc
     4441 gttttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc
     4501 gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac gcagcaacac
     4561 gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa ctgactcatg
     4621 ctcaagcgga tcgccgctac ctgcggtgta acgcacgtta accagaatct gcgggaacat
     4681 tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg ccgccagcac
     4741 ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc
     4801 ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc
     4861 acccactttc gcgcgcgcaa atggaatacc taactgtttc agcgccagct caagccccat
     4921 gttactcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa gaccttcacg
     4981 cgcgatgata tacatgatct gatcaccatc gactttattg ccttcatggt caaccataat
     5041 cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg
     5101 agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgttcgg
     5161 ctcacaaccg atagcgataa cgttcgcccc cagttcacgc agcacgttcg gcgcgatgtg
     5221 ataagtcgca ccgtttgcgc aatccaccac aatcttcagt tcgctgaggc caagttcgtt
     5281 cgggaacgtg gctttgcaaa actcgatata gcgacccgca gcatcaacga tacggctggc
     5341 tttacccagt tctgccgaat caacacagct gatctctttt tccatttccg cttcgatggc
     5401 ctcttctacc gcatccggta gtttggtgcc gtcgatagag aaaaatttaa tgccattatc
     5461 gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt
     5521 cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc
     5581 cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc
     5641 aataatgatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt
     5701 aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc
     5761 gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca
     5821 cccgcatcgc ttctacggtt tctttgacgt catgaacacg aataatgtgc gcgccttgca
     5881 ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc cccacgttca
     5941 gcagctgccc aatcatcgat tttcgtgaca tacctaccaa cagcggcagg ttgaaatggt
     6001 gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga
     6061 atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt
     6121 gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga gcttcctgca
     6181 tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgccgccg
     6241 cctccagagc gccaggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt
     6301 tcgctgactc acggatgact tctggtttgg atgtatctac cgagagccag acttcgaagc
     6361 gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt
     6421 ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga
     6481 tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa
     6541 aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag
     6601 tgccctgggc aaagagtttc atggtgttat ccctggtatg aattgataag aaaaaacccc
     6661 ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa cttacttgtc
     6721 gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg
     6781 aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc
     6841 cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt
     6901 ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag
     6961 ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg
     7021 tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga acacttcacc
     7081 ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat
     7141 attacgcgcc aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc
     7201 cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc
     7261 cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag
     7321 cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga tcgtgttccg gcaccaagcg
     7381 accgataatc gcatgacccg cttcgtggta agccgtcgat tctttctgcg cttccgtcat
     7441 caccatggag cgacgttccg cacccatcat gattttatct ttcgcttttt cgaactcaat
     7501 catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt ttaccaggtt
     7561 cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc
     7621 gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc
     7681 tggcaagcca accacaacct gacggtcgaa acggccagga cgcagcaggg ctgggtcaag
     7741 aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc
     7801 catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc
     7861 agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc
     7921 tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc
     7981 tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt
     8041 cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt
     8101 accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt cagcaacttc
     8161 ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt
     8221 cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat
     8281 ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc aagagatgaa
     8341 gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt
     8401 caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact
     8461 atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt
     8521 attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc
     8581 aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt
     8641 cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat
     8701 actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc
     8761 ggtcgctaca atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt
     8821 gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa
     8881 caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt ctaacgccag
     8941 ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc
     9001 catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat
     9061 caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg
     9121 taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga
     9181 ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc
     9241 actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc
     9301 ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg
     9361 cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat
     9421 ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc
     9481 caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc
     9541 tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc
     9601 tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg
     9661 aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta
     9721 atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct
     9781 cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt
     9841 tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcatagcggc
     9901 ctttttcctt tctttacaat acatcaacat cttgatgtat tgggtaattc ttacaggtat
     9961 tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac cacaacatca
    10021 tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat caggttttgt
    10081 ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc agaatccaga
    10141 ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat tttggtgaca
    10201 tcaatcacct gcgcgttcga cagcttggct tcgatgtctt taatacggcc ttcgcagaaa
    10261 ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc
    10321 gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa atccagctct
    10381 tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg ttatacctct
    10441 tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg cccggcataa
    10501 ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc aggtcagcta
    10561 caattcacat tttgatagtc attttaccct gaagttcccg aagggtcatc gtttacttta
    10621 tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga gattatgcga
    10681 ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca ggccgcgaat
    10741 gttgatgagt acgttactca actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa
    10801 gtcggcgcgt cggcccccgc tattgattac cacagtcagc agatggcgct gcccgccagt
    10861 acgcagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga ttttcgtttc
    10921 accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg
    10981 gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat gatcgcgact
    11041 ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac ctccattttc
    11101 gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg ctttagcgct
    11161 ccgcctgccg ctgccatagt tgaccgcaac tgtttctccg tctcgctcta cagtgcccca
    11221 aaacctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac gatgttcagc
    11281 caggtacgca ccctcccccg aggttctgcc gaagcgcaat actgcgaact ggatgtcata
    11341 ccaggcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc tgagccgctc
    11401 ccgttggctt ttgccgtgca ggatggagcc agctatgccg gggcaattct gaaagatgag
    11461 ttaaaacagg caggtatcac ctggagcgga acactgctgc gccagactca ggttaacgaa
    11521 cctggaacgg tagttgccag taaacagtcg gccccgttgc acgatctgct taagattatg
    11581 ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatagg ccatgcgcgc
    11641 ttcaatgtgc ctggaacatg gcgggccggg tcggacgccg tgcgtcagat cctgcgccag
    11701 caagccggtg tcgatattgg aaacaccatt attgccgatg gttcagggct ttcgcggcat
    11761 aacctgattg cccccgccac catgatgcag gtgttgcaat acattgctca acacgacaat
    11821 gaacttaact ttatctccat gctgccgctg gcgggctatg atggctcttt gcagtaccgt
    11881 gcaggtctgc atcaggcggg cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag
    11941 ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat ggcgtttgta
    12001 caatatcttt ctggctatgc agtagaaccg gcggatcagc gtaatcgccg tattccgtta
    12061 gtgcgttttg aaagccgttt gtataaagat atttatcaga acaattagtc aaaaagaaac
    12121 cccggcacat ggccggggtt ttgctgatta acgcttgtaa atgaactcaa cgccttcttc
    12181 gtcgtcttcg tcccagtcat catcccagtc ctcatcatcc tcttcagcaa tctcttcaag
    12241 ctgctggcga tgataatcat cccacatgaa ttcgactttc tctggctgtt tcgcttcttc
    12301 agcctgcacg accgggtttt caatgataaa ggtcatcaca tcccagcaga gatctttcac
    12361 gcccagtcca ctcgccgcag agatcagata atatttatct tcccagccca gcgcttcagc
    12421 aatcgctttc gctttctctt cagcttctgc cttatccagc agatcgatct tattgaacac
    12481 taaccaacgc ggtttcgccg ccagatcctg gctgtatttt tccagctcgc tgataataat
    12541 acgcgcgttt tcaaccggat cggtgccgtc aatcggatcg atatcgatga ggtgcaacag
    12601 gacgcggcaa cgttccaggt gcttcaggaa gcggataccc agacctgcgc cttccgcagc
    12661 gccttcaatc agtcctggaa tatcggcaac aacgaagctc ttttcgttgt ccattcgtac
    12721 cacacccaga cttggcacca gagtggtaaa cggataatcc gccactttcg gtttagccgc
    12781 cgataccgcg cgaataaagg tcgatttacc cgcgtttggc atccccaaca taccgacgtc
    12841 agccagcagc atcagctcca gcagcagctc acgcttatca cccggcgtac cgttggtttt
    12901 ctgccgtggt gtacggttaa cggacgattt gaaacgggta ttgcccagac cgtgccagcc
    12961 gcccttagca accagcagac gctgaccgtg tttagtcata tcgcccatgg tttcaccagt
    13021 accctggtcg attacacgcg tacctaccgg cactttaatc gtcacgtctt taccgcgctt
    13081 accggtacaa tcgcggcttg cgccgttctg accgcgctct gcacggaaag atttttcaaa
    13141 acgataatcg ataagcgtgt tcaggttttc gtcggcttcc atccatacgt caccaccatc
    13201 accaccgtcg ccgccatccg ggccgccttt cggaatatac ttttcgcggc ggaagctcac
    13261 gcaaccatta ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa caaacttcat
    13321 tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg tatgcttacg
    13381 taatccgccc caaatacgat gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa
    13441 cgcaccgaga taacctaaaa ggtttaacat cggtctggcg aagaaatcgg gccaggccag
    13501 tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac
    13561 ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg ccatatccta ccaatgtatt
    13621 cagtccgcaa aaaattaaac atgcgagctg ccagtggcta agctgcgcta tcactcctgg
    13681 ctttgccaga gggaagagcg caattgtaca taaagtgtac agcaaaaaca ggatctgcgg
    13741 tgaggccagc cgacgcaata aaaccttttg cgccacgcca taactcaccc aaaccgtcgc
    13801 cgcaccgacc ccaaagatga ctccccaggt gtaatcagtg agctttgtaa atatctcgac
    13861 cagactggtg ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc ctacaacctg
    13921 agtgctgcgc attttctctt tcaggataaa tacgctggca accatcatgc caactggcga
    13981 gagttgccca atcacctgcg aagcggtcgg actcaggtat tgcaaggatg agctgaacag
    14041 gatgaagttc ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc agcgtggctt
    14101 acgaaacacg cgtaatggcg gcaacctctt cttcaccgca agaatggcac ccaggccaat
    14161 actcgccatc aagaaacggt aaaacacgat tgtcggaggt tccatcacct ccagcacctg
    14221 cttcattgcg attggcaacg ccccccagca aattgctgtg gtgagcgcca aaagaatgcc
    14281 aatgcctgcc tgctgcttca taccgtattc cctgcaaagc gcatttgcgg attaacgtcg
    14341 caccatgctc atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct
    14401 ttcatccgtt accgggacgc gaaaaactta ttcagcttcg atgctgataa atttacggtt
    14461 tttcgggcct ttaacttcga atttcacttt accgtctgct ttagcaaaca gagtgtggtc
    14521 acgaccgcaa cctacgttag cgccagcgtg gaatttggta ccacgttgac gaacgatgat
    14581 gctacccgcc agaacggatt cgccaccgaa acgcttaacg cccaggcgtt tagcttctga
    14641 atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt gccatttgaa atctctcctc
    14701 aggtcttagg cgctgatgcc agtaattttc acatcagtga accactgacg atggccctgc
    14761 tgcttacgat agtgtttacg acgacgaaac ttaacgattt taactttctc gccacgaccg
    14821 tgagcaacaa cttcagcttt gattacgccg ccatcaacga aaggaacgcc gattttgact
    14881 tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc agttgcgatg
    14941 tccagctttt ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt accaccactt
    15001 tggaaaaccg cgtacatata aaactccgct tccgcgcaca ccttttcaat gattcagagt
    15061 gcgctataaa tattcacaat agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt
    15121 gctacagtca atacacgaag aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca
    15181 ttttttcagt acaatcaccc tatattccta accataaacc ctaagttgcc tttgttcaca
    15241 gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt
    15301 taaccgcgca agatatggcg ggtgttaatg cggcaatcct tgagcagctt aattccgacg
    15361 tccaactgat caatcagtta ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc
    15421 cgatgattgc tgtactggct gcacgagctg ttggctatga gggaaatgcg catgtcacca
    15481 ttgctgccct gatcgagttt atccacacgg cgactctgct acacgacgac gttgtggatg
    15541 aatcagatat gcgcaggggt aaagctaccg ccaacgctgc atttggcaat gccgccagcg
    15601 tgctggtagg cgattttatt tatacccgcg cgttccagat gatgaccagc ctcggttcac
    15661 tcaaagtgct ggaagtcatg tcagaagctg taaacgtcat cgcagaaggt gaagttctgc
    15721 aactgatgaa cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc gttatctata
    15781 gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc cgggattctg gctggctgta
    15841 cgccggagga ggagaaaggc ctgcaggatt atgggcgcta tctcggcact gctttccagt
    15901 tgatcgacga tttactcgat tacaatgccg atggcgaaca gttaggtaaa aacgtcggcg
    15961 acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca tgcgatgcat catggcacac
    16021 cagaacaggc acagatgatc cgtaccgcca tcgaacaggg taacggtcgc catcttctgg
    16081 aaccggttct ggaagcaatg aacgcttgtg gatctcttga atggacgcgt cagcgcgccg
    16141 aggaagaagc agacaaagcc atcgcagcgt tacaggtgct cccggacacc ccttggcgag
    16201 aagcactcat cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc cctcccttca
    16261 tcccgcgcag tgcgcgggat gattccagta aattccataa aaacactaat tcagcgctat
    16321 aacacctgaa aaaaagggcg atgaatcttt atatattctg aatattcaca ctctttacag
    16381 gaacttttta gagcaatagg ccatcaggag tatagtgatg cccgacagaa gaagtgttct
    16441 gaatgaaagc gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca
    16501 tccccctgac atcattgcag gtttgcgcaa gaaaggaacg tcaatggcgg cggaatctcg
    16561 cagaaatggt ttgagttcct caacgctggc aaacgcatta tcgcgcccat ggccgaaagg
    16621 agagatgatt attgcgaaag ccctgggaac tgacccctgg gttatctggc catcacgcta
    16681 ccatgatccg cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa
    16741 accgaaaaaa tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacga
    16801 ttactcgcct ttcacacgct taatatttgc acctaaagcg cgcagtttgt cttcaatgcg
    16861 ttcgtagcca cgatcgatgt gataaatacg atcaaccacc gtcgtccctt ccgcaataca
    16921 gccagccagc accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcaccaga
    16981 aagtttttca acaccatgac aaataacggt attgctttcg atttcggcat gcgcgcccat
    17041 acggctaagc tccggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaatcc
    17101 ggtcccttct gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc
    17161 cggatgcggc gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct
    17221 aatccagtct tcgccgactt cgatgtccgc tccagcgtca cgcagtttcg ccagcacggc
    17281 gtcgagagta tctggctgcg cgttacggca gataattttg ccgcgagaaa tcgccgccgc
    17341 caccaggaaa gtaccggttt cgatacgatc cggtagcacg cgatagacac cgccgcctaa
    17401 acgttccaca ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag
    17461 cgtaatcagg aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat
    17521 cgtggtacct tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac
    17581 tttatccatc acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc
    17641 ttcttccagt ttgatggtcg cgcctaattg ttcgaggcca gaaatgtgta gatcaaccgg
    17701 acgcgcaccg atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa agcgcgctac
    17761 cagcggcccc agtgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca
    17821 gaatacatta acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcacc
    17881 cagctgactt agcagcttca ttgatgtatc gacgtctttc agtttcggga cgttctggat
    17941 ctctaccggt tcttccgcca gtagagcggc aaaaaggata ggcagagcag catttttagc
    18001 gccggaaatt gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat
    18061 ttagtttgtt ctcagttaac aattcatatc cgctaccggc gaatcgccca tagctcaaaa
    18121 gccgttcagt ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc
    18181 atgaatgcgg ttatccgcaa tatattccat cagcgggcca tagaccgtct gctgtttttt
    18241 aacccgactc atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc
    18301 gccggaaacg tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt
    18361 attttccatg ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt
    18421 tgcgctggca taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag
    18481 gcaggtttgc cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag
    18541 aacatcagca ggcaaattat acaatttcgc cagggtatac actttgtcgt ttaccccctg
    18601 aagcgtcaca ttgttgccct gctttttcgc cagatcgata agatggagca gcagtgccag
    18661 tccccccgta tccacgcggg acacacggct aagatcgatg caggtaatcc ccttcaccgc
    18721 ttcctcacgc atttcccaaa gcggtagcaa aacgtcctga tccagctctc cggataacgc
    18781 cagcgtgtca cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga
    18841 gtgattttct gttgagaaat cgatttcagt tgcgcagtca ggccgtcgat acctttggta
    18901 cgcagcagcg ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc
    18961 atgtcgtaag cctgccagtt gcccgtctgg gagtttttac gccactggaa gtccagacgc
    19021 accggcgggc ggccattcgg atcaataatg gtaacgcgaa tcggcacaat ggttttatcg
    19081 cccagcggct gttctggcgc aatctgatag gtttgaccat gatacatcgc cagcgcctga
    19141 ccgtaagcct gcttcaggta ctcacggaaa gcggcaaagt aggcttcacg ttgagcaggg
    19201 gtcgcactct tgtaatactg gcccagcacc agcgcaccgg cgtatttcac ctgtacgtat
    19261 ggcagcagtt cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgcggt
    19321 tgctcattct tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttataagga
    19381 ttggtctggt ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa agcgaccatc
    19441 attaaacgtt taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca
    19501 gtggtttcat tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg
    19561 cctttactac cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc agacttagtg
    19621 tcctgaattg tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg
    19681 acgtttaatg ccagatattg ttcccccagt aggccggaag tacgaatgct cagcgaactg
    19741 gtatctggaa tgtggttata acgttgttca atttccagcg ttacgcgtgg cagataggtt
    19801 ttcgggtcca gcgtaatatc cgccacccgg cccacaacaa cgccaccaat actgaccgga
    19861 gagcgcgctt tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca
    19921 gtacgtatgg acgtcacgtt cgccgccttc aggcaaacaa acagagccgc cagcagtgct
    19981 gctaataaaa agatacccac ccaaatttca ttttttttcg tttgcatgta ctcaattccc
    20041 aaacatcaat gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac
    20101 ggtgcgagtg gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa
    20161 caacgaaatc cacgtcaccg tgatggcgaa caccacgctc ttaatcagac agttgaccag
    20221 atccatacgc cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc
    20281 tttccaactg acgccgacca gagatccgcc ccagatcccc acggcgacga aaataaccgt
    20341 caacagtggt aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg
    20401 atccaccgcc atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc
    20461 tgcggttagc gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag
    20521 ttcacgcagt agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt
    20581 cagaaccaga taaccttgca gccccaacac cattccgatg aacacgccag aaaccacaat
    20641 aatcagcatc gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt
    20701 gcgaaattcc ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccgaacgt
    20761 tctcagggtt ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg
    20821 cttaactccc tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaacag
    20881 gcccgtcagc tatcccgtcc agaaactgac gtacgcgcgg atcaggattc gcctgcaacg
    20941 cctgggcact gccatgagcg acaatttttt tgtccgccag gatccaggcg tgatccgcaa
    21001 tacttaacac ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc agcgcgctgt
    21061 tcagctcaga aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaaag
    21121 gttcatcaaa catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc
    21181 gcgccatccc accggaaagt tcagaaggca ttagtttagc cgctccacgc agccccacgg
    21241 cctccagctt catcatcacc gtactatgca acaatggcgc gggaagttgg gtatgttcgc
    21301 gcagtggata ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc ccggactgaa
    21361 ataacatgct catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat
    21421 tctcaccatc gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga
    21481 gtagcgtcgt tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca
    21541 cggtcaggga aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca
    21601 tatcgactaa attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc
    21661 taagcatggc gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg
    21721 gttagcgaaa gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc
    21781 gttacggctt tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggacgttag
    21841 atgcttttag ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac
    21901 cgcctggttt ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctgatcatc
    21961 ggcatgacgg tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc gcttgccgcg
    22021 tctctgcacg aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat
    22081 attttgctga tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttccgatgtt
    22141 ctgcgtcgtg aattaccctt aatgttgttg gtcagcgtag tggccggttc cgtactctat
    22201 gacggacaac ttagtcgcag cgatggtatc tttctcctct ttctggctgt gctatggctg
    22261 ctgttcattg ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga
    22321 gagcagcttg cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt atggctcggc
    22381 attgcgctta tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg
    22441 gcgaattact ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc
    22501 agcctgccgg aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa cgacattgct
    22561 gtcggaaata tcattggcgc aaacattttt aatattgtca tcgtgttggg tttacccgcg
    22621 ctgataacac caggagagat tgatccattg gcgtacagtc gtgactacag cgtgatgttg
    22681 ctggtgagca ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc gggccgtggt
    22741 gtaggggtat tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg
    22801 ccaatactcg ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt
    22861 tgactttcag caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct
    22921 tgatcaatac atcaatcaga atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg
    22981 gaaagttgtc gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac
    23041 gtttgccagc accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga
    23101 tttaggcatg gtcaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag
    23161 cgaaatcacg gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa tctgcatcac
    23221 cggtcgcccg gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc
    23281 gaaagaagcc tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat
    23341 gggcgatgcc ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc
    23401 gctctcacac ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat
    23461 gcatacgggc gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct
    23521 ggaagttacc cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata tgatgattga
    23581 aggcatcttt accgacggtg atttacgccg tgtcttcgat atgggagtgg atgttcgtca
    23641 gttaagtatt gccgatgtga tgacgccggg gggaatacgc gtgcgccctg gcattctggc
    23701 cgttgaggca ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg
    23761 cgaccattta ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag
    23821 attcaaggat aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct
    23881 gtcagcgccg acgttatagc aaaagcagag aacattcgtc tgctgatcct cgacgttgat
    23941 ggcgtactgt cagatggcct gatttatatg ggcaataacg gcgaagagct gaaagcgttc
    24001 aatgttcgtg acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc
    24061 attaccgggc gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac
    24121 ttgtatcagg ggcagtcaaa caaactgatc gcctttagcg aactgctgga aaaactggcg
    24181 attgccccgg aaaatgtggc ttatgtcggc gatgatctca tcgactggcc ggtaatggaa
    24241 aaagtgggtt taagcgtcgc cgtggccgat gctcatccac tgttgatccc gcgcgccgat
    24301 tatgtgacgc gcattgctgg cggtcgtggc gcagtgcgag aagtttgcga cttattactc
    24361 ctggcgcagg gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt
    24421 gggttaccat tgtgctatca ctggcggttc tggtgatgat cggcattaat atggccgaaa
    24481 aagacgatac cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata
    24541 cggacacgct cgtctataac ccagaagggg cactaagcta tcgattgatt gctcaacacg
    24601 ttgaatatta ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg
    24661 ataaggataa aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc
    24721 ggatgctcta tttatatgga cacgttgaag tcaacgcact tgtgccggac tctcaacttc
    24781 gcagaatcac gacggataac gcgcagatca atctggtgac gcaggatgtt acctctgaag
    24841 acctcgtcac gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact
    24901 tacgcagcaa gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca
    24961 aacaaactca gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca
    25021 tttgccgtaa ccggagacac tgatcagccg atccacattg aatcggacca gcaatctctt
    25081 gatatgcaag gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca gggcaccatc
    25141 aaaattaatg ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg taaagaagtg
    25201 attgacggct acggtaaacc ggcaacgttc taccagatgc aggacaacgg taaacccgtt
    25261 gaaggtcatg cttcccagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt
    25321 aatgcttatc tgcagcaggt cgatagcaac attaagggcg ataagatcac ttacctggtg
    25381 aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaacaac cgttctggtg
    25441 ccgtcgcagc tgcaggacaa aaacaacaaa ggccagaccc cggcacagaa gaagggtaat
    25501 taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc
    25561 gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca
    25621 aacggtgccg gtaagaccac cactttctat atggttgttg gcattgtgcc gcgcgatgcg
    25681 ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc acgcgcgcgc
    25741 cgcggtatcg gctatctgcc acaggaagcc tccattttcc gtcgcctcag cgtttacgat
    25801 aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg tgaagaccgc
    25861 gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca
    25921 ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa
    25981 tttattctac tcgacgaacc gtttgccggg gttgacccga tctcggttat cgacattaaa
    26041 cgcatcattg aacacctgcg cgacagcggc ctgggcgtac tgatcactga ccacaacgtg
    26101 cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgattgcc
    26161 cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa
    26221 gacttcagac tctgataggg tagaagtttg cgacgtttta gcaggagagt acgattctga
    26281 acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagcttc
    26341 aacaggcgat tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg
    26401 cgctggagag taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacaccagcg
    26461 aaacgcaaga cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg
    26521 aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca
    26581 ccagcggtga ctacattgac gatgagctgc cggtctatca gggcgaaacg acgcagacct
    26641 tgcaggatta cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta
    26701 ttgctacctc tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag
    26761 atattctcga aagtatgggc gatgaagaga tcgacatcga cgaggttgaa gccgtcctta
    26821 agcggatcca acggtttgat ccggtcggtg tagcggcaaa agatctgcgt gactgcctgc
    26881 taatccaact ctcccaattc gataaaacca cgccgtggct ggaagaggcc agactgatca
    26941 ttagcgatca tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc
    27001 gtctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc
    27061 ccgggcagtc gatccagact ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta
    27121 agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt ctgcaaatca
    27181 accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgacagc cagtttatcc
    27241 gcagcaatct gcaggatgcc aaatggttga tcaagagtct ggaaagccgt aacgatacgc
    27301 tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggtgaag
    27361 aatatatgaa accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga
    27421 cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga
    27481 agtatttctt ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga
    27541 ttcgtgcgct ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca
    27601 gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga
    27661 agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca
    27721 accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag
    27781 gcactgcgcg aatttgttag agccaaattt gccaaacttg agcaatattt tgaccgaatc
    27841 aaccaagtct atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg
    27901 catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt
    27961 gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa
    28021 cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca
    28081 tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa
    28141 attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg
    28201 cgaagccgcg ttcactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg
    28261 gcgaaacaac ttagcctgcc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa
    28321 atgggcagta ccggtatcgg caatggtatt gccattccgc atggcaaact ggaagaagat
    28381 actctgcgcg ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc
    28441 gacaaccaac cggtggactt actttttgcc ctgctggtgc cggcagacca aactaaaacg
    28501 cacctgcata ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt
    28561 ttacgcgcag cccagagcga tgaagagctg tatcaaatca ttacggatac cgaaggtact
    28621 ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac
    28681 atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg
    28741 ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg
    28801 gctcgaaccc tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg
    28861 ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg
    28921 caactcctgt tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc
    28981 cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc
    29041 gatttgctgg agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc
    29101 gttcacgagc tggctgaaat gctgcgtacc cgtctgctgg gtaaacgtga acgcgaactg
    29161 accatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc
    29221 tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt
    29281 cttgataaac ctgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac
    29341 cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac
    29401 ttgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa
    29461 ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa
    29521 aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg
    29581 cccggcctgc aatgaagctg tttgaattaa tgcagggttt tgacgctgaa gtgctgttac
    29641 gtaatgacga aggcaccgag gccgaagcca acagcgttat tgcgctgctg atgttggatt
    29701 ctgccaaagg acggcagatt gaagttgaag cgaccggccc acaggaagag gaagcactgg
    29761 ccgccgttat cgccctcttt aactctggtt ttgatgaaga ttaatcttca tcactttgac
    29821 atacaactcc cttcaaactc cccccgacaa taagaaaatc acgtactgaa atcgttctca
    29881 atcaacgtca tttgtacatt ttgtgcgctt ttcactttca gaagaacctt aagaaaacct
    29941 taagaggtat tgtttaggtt ttgtttaagt taatcgacca tactggagat cgtcagaaaa
    30001 tatttccagg agatggcatg attcgcttat ctgaacaaag tcccctgggc accggaagac
    30061 atcggaaatg ttatgcgcat ccggaagatg cccaacgctg tatcaagatt gtctaccatc
    30121 gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa gtactacgcg tatcttggtc
    30181 gccggttaaa agactggagt ggaataccgc gctatcacgg taccgttgag acggattgcg
    30241 gaaccggtta tgtctacgat gtgattgcag attttgacgg caaaccctcc attacgctga
    30301 ccgaatttgc tgaacaatgc cgttacgaag aagacattgc acaactgcgc cagctactga
    30361 aacagctgaa gcgttatttg caggataacc gtatcgtgac gatgtcgctg aagccgcaga
    30421 atatcctctg tcaccacatc agtgagtcag aggttatccc ggtcgtctgc gacaacattg
    30481 gtgaaagcac attgattcca ctggcgacct ggtcgaagtg gtgttgtctg cgtaagcaag
    30541 agcggttatg gaaacgattt atcgcccaac cggcgctggc tattgccctg cagaaagatc
    30601 tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg cgaagcttaa tccagctggt
    30661 gttgttgcat aaacggctca ccgcctaact gatacatctg ccgtaaaatc cacgcctgac
    30721 ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg aagtggatta ggtaatacag
    30781 ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc gggtttgtgg aaataacgtt
    30841 gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc ggcgatattc aggtaaaccg
    30901 tcaggatacg ctttttgctc cagactgttt ctatccccag cgttaatcca gcttccagcc
    30961 cttttcgtac ccagctacgc ccatcccata aaaagagatt tttggccgtc tgctgagaaa
    31021 tcgttgaagc accgcgaata cggttttcat tgcgctcgtt gtgcgccagg gctttctcaa
    31081 tggaagcgac atcaaagccc cggtgctcag gaaatttctg atcttctgcg gcaatcaccg
    31141 ccagtcccat ccacggcgag atttgatcca tactgaccca gtcagaatgt gccacgtaac
    31201 gaaaattgcc atgcagccag gcgctgacct gtcgctcgac cattaccgct gagaagggaa
    31261 caggcgcaac actaaacaac gcgatgcccc cgccccagaa aacggcgagg acaaccatta
    31321 accgcaaaag aaaacggcga acgaaactaa acaccgttaa gcggctttta ctcattcagc
    31381 cagaaccagc acgcgggaaa ccagcttatc aatgccgctc gccgcttctg caatgttctg
    31441 cgccagcata tatgctgggg tggtgacaat cttattgtct tcatcaacca cgatatcatc
    31501 gacaggacag ggcacatgct ccgcgcccat ctcttccagc acttctgcgg tatcgatatc
    31561 agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat
    31621 acacataaaa ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg cctttaattc
    31681 acggtcaacg gtgcattcgc taccaagagt ggcaaaattg cttaaattct tcgccgcgcc
    31741 aaaccccccc ggcacaatca acgcatccag ttcagcggca tcggcctggg ccagaggacg
    31801 gatttcaccg cgcgttattc gtgccgcttc aatcagcacg ttgcgcgttt ccgtcatcgc
    31861 ttcgccagtt aaatggttga taacatcaac ctgctgctta tccggtgcaa agcagaccgc
    31921 ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc
    31981 gtcatagacg ccgcatccgc tcagaattac gccaattttc ttcattgtga tcatcctttc
    32041 gcaattgact gaaacacatt aattttttta ataaaaatgg tacgcatcac acatttaact
    32101 gattcatgta acaaatcatt taagttttgc tatcttaact gcgtgcggcc tgaaaaacag
    32161 tgctgtgccc ttgtaactca tcataataat ttacggcgca gccaagattt ccctggtgtt
    32221 ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt tttagtggct tttgccaccc
    32281 acgctttcag cacttctacg tcgtgacgcc actcttcttt catctcttca atccattcac
    32341 cgacgttatc ttcccaggca ggaaggtcag gagactgaat ttgctgaccc agctgttgca
    32401 gatggcgtaa ccccactgac cccgccgcac ctttaatttt atgtccttcc tcaacaatgc
    32461 cttttttatc ctgcgccgtc agattcgact ccagcacgct gacatagccc ggcatcatct
    32521 tctcaaacac cgctaacccg tcggtgatca gcttcggtcc tacaagttcg agatactgtt
    32581 ccagcatggg aatatcgagc aatgcttctg atttactgtt ctcttctgtc gtcaccgtac
    32641 tctcctcatc atcctgggta tcccagaatt tcttgatcat cgcggttagc gccggaaccg
    32701 aaagcggctt actcagcaca tcatccattc cagcattgag gtactcttgt ttgtctttca
    32761 gcacgttagc ggttaaggcc accagcggcg gtaaatcctc gcgcggataa cgcttcgtca
    32821 gttcacgaga gatatccagc ccggtcatat ctggcaactg aatatccagc aacaccaggt
    32881 cgtattcgcc cggtttaaac atctccagcg ccgccttgcc ggtcatggcg acatcaacgc
    32941 tgttacctaa tttttccagc acagaacgcg caacaatcac gttcagttca atgtcttcca
    33001 ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct
    33061 cttctgctac cgacggtgcg tggattgtca acgtaaaggt tgaacctttg ccctgttcgc
    33121 tggtaaccgt aatatcgccg cccatatttt tcgccagacg acgagaaacg gccagaccaa
    33181 taccggtgcc ggtggcaggt ttaccgccat gactgtcttt cacctggtaa tacatggcga
    33241 aaattttatc cagctcatcc tgcggaatgc cgataccaga gtcttccact tcaaaatgca
    33301 gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg gtgaatttga
    33361 cggcgttact gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct
    33421 gatgcggtaa tggcagcgtc ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca
    33481 atgcggagag attttccaga tcggcaagga agctggtgaa atcaaccggt tgattatcaa
    33541 gctggacctt gcgccgttcc atcttatcca tgtcgataat atcgttaaag atattcccca
    33601 gcgtgacggc cgaaacatgg atggtcttga gatatttttc ctgctcggcg gtgagttcgg
    33661 tatccagcag aatgcggctc agaccgacga taccgttcag cggtgtacgc aattcgtgac
    33721 tgatggtgga gataaacgtc gttttgtcgc ggctggcccg ttcaagcgca tcctgatacc
    33781 gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt
    33841 cgtagtacgg cactttacgg atttcaaagc aggctttgcg cccgtccggg taatccagcc
    33901 actgttcata ggtcagtgac acattatgac ggaacacttt ttcatcggtt tcaatgactt
    33961 ttgcggcggc ttccggtgag taaacatcag caggtttcag gtgaaccagt tgtttttcgc
    34021 tttttccggt cagcagctcc atcgcgcggt tacagccgga aaactcttta tcttcgttac
    34081 gataaaaaac caggtcgggt gaagcatcaa ggaaggaacg taagaatgag gattgctgct
    34141 cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt
    34201 cctgcagttc tgcttccgct ttttcacgaa cggcaatttc ctgatttagc tgggcaatat
    34261 tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc agtttttgca
    34321 ccagccgtga cagacgttgt cgtgactcct ccagttgctc gacgaccacc gatagaaagt
    34381 agaccgccca cggcgtaatc agcaaaccaa agaagataga acgaataaca tcaatgcttt
    34441 cgacctgacc atgcagcacc atggttaccg ccatttgtac cacaatggca agaacgacga
    34501 gggccagcgc cagcaacatt gagaagcgca ccagacctaa cttcatcatc aggtcaacat
    34561 aatactgcgc cagcagacga atttgcttca ttagggaatt ccttcacgac aacctgcccc
    34621 aataataccc aattctgaca gttacgttat aaattgtgtg agaaatgcgg aattcccctc
    34681 aacgacctac tccgttggag gtagccacgg acgtcccagc gccgatccct gtacgccatg
    34741 ttcattcaga tagcgatcca gctcgaccat tcccgtccat cgattttcgc accacagcgg
    34801 agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca cttccggtgg
    34861 cgtatggcga atcatttctc cggcagtgag cgtgtaatcc tccagttcaa taccgttcaa
    34921 acgccccgct tcccaggctt tcgccataat gctgcctttc acaatatgca gcggatgcag
    34981 ctttatgccg tccacgccgg tttcaaccac tcgctccagc gtttgcaaac attcagcctg
    35041 cccttcacca ggcaggccga caattaagtg ggaacacacc ttcagcccgc gctgacgtgc
    35101 cagctgggtt gtacgctgat aacaggcaaa atcatggccg cggttgatgc gatgcagtgt
    35161 tttgtcgtgg gcggtttgta gccccagctc cagccacact tcgtaaccct ggtccttata
    35221 ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc gggcgggtac caacacacaa
    35281 accgacaata ttggcctggc tcaccgcctg ctgatacata gaacgcagca cctgaacttc
    35341 cgcaaaggtg ctagtatacg cctgaaagta ggccagatag cgtttagcgc ggttaactaa
    35401 attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc tgctgcgctt catcggcaaa
    35461 cgaggcaaca ttacagaatg tgcagccacc acgcccgatg gtaccgtcac ggttagggca
    35521 gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc ccataacgac gggtgagatc
    35581 accaccaaac atattgacta atttctgtaa ctgcataatc tgatagaccg cgccttgaaa
    35641 agaggccaaa gcctgccatt tttagctcaa ttcggcgatg acctggatca atcgtcccgg
    35701 cctgctttta tcaactgcat aatcaatcaa aattaccgaa atttcatgca taatcacata
    35761 aatcactttt gcttatcttg tgtcagattt ttttatctcc ttatggattt taggcaaaaa
    35821 cagtagcatg aaacgtcatt accaattaag gcagtataaa atgctggttt tctcgtcagt
    35881 tcaaggtagg ataagggtta acacgccttt atgacagtca ggaattgact gtttctctaa
    35941 cgacttccct tttagcctta aagataaaat tcattttaat ttcagttact taataaagaa
    36001 ttttgcgcta aagcacattt ctgtaccaat aagcttgcca tttgacctgt atcagctttc
    36061 ccgataagtt ggaaatccgc tggaagcttt ctggatgagc agcctgctca tcatatttat
    36121 gcagtaattg agatcccctc ttcaccgtat taaccgatgc gaaaaggaca acaagggggc
    36181 gaatacgagg cgcgcgtatg acacgcaacc cccgtcgcca cgctctttct gtgcccgtgc
    36241 gcagcggttc ggaagtgggg ttcccgcaga gcctggggga ggttcacgat atgttgtacg
    36301 ataaatccct tgagagggat aactgtggtt tcggcctgat cgcccacata gaaggcgaac
    36361 ctagccacaa ggtagtgcgt actgcaatac acgcactggc ccgcatgcag caccgtggcg
    36421 cgattctcgc cgatggtaaa accggtgacg gttgcggctt gctgttacaa aaaccggatc
    36481 gcttttttcg catcgttgcg caggagcgcg gctggcgttt agcaaaaaac tacgctgtcg
    36541 ggatgctctt cctgaataaa gatcctgaac tcgcggctgc cgcacgccgc atcgttgaag
    36601 aagagctgca acgcgaaacc ttgtcgattg tgggctggcg tgatgtcccc actaacgaag
    36661 gcgtgctggg tgaaatcgcc ctctcctctc tgccccgcat tgagcaaatt ttcgttaacg
    36721 ctccggcagg ctggcgtccg cgggatatgg agcgccgtct gtttatcgcc cgccgccgca
    36781 ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg tagcctgtcg aatctggtga
    36841 acatctataa aggtctgtgt atgccggcgg atctgccgcg cttttatctg gatcttgcgg
    36901 acctgcgtct ggaatcggcc atctgcctgt tccaccagcg cttctccact aacaccgtac
    36961 cgcgctggcc gctggcgcag ccgttccgct acctggcgca taacggtgaa atcaacacca
    37021 tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa attccagacg ccgcttatcc
    37081 ctgatctgca cgacgccgca ccgttcgtca acgaaaccgg ctctgactcc agttcgatgg
    37141 ataacatgct ggaactgctg ctggcaggcg ggatggatat catccgcgcc atgcgtctgt
    37201 tagtgccacc cgcctggcag aacaacccgg atatggaccc ggaactgcgt gccttctttg
    37261 actttaactc catgcatatg gagccgtggg atggcccggc gggcatcgtg atgtccgacg
    37321 gtcgttttgc cgcctgtaac ctcgaccgta acggtctgcg tccggcgcgc tacgtcatca
    37381 ccaaagataa gctcatcacc tgcgcctctg aagtcggtat ctgggattac cagcctgacg
    37441 aagtggtcga aaaaggccgc gtcgggccag gcgaactgat ggttatcgac acccgcagtg
    37501 ggcgtattct gcactcggca gaaaccgatg acgatctgaa aagccgccat ccatataaag
    37561 agtggatgga gaaaaacgtc cgccgactgg taccgtttga agatctgccc gatgaagaag
    37621 tgggtagccg cgaattggac gacgacacgc ttgccagcta ccagaaacag tttaactaca
    37681 gtgcggaaga gctggactcc gtaattcgcg tactgggcga aaacggtcag gaagcggtcg
    37741 gttcgatggg cgatgatacc ccattcgccg tgctctccag tcagccgcgc attatttacg
    37801 actacttccg ccagcagttt gcccaggtga ctaacccgcc aatcgacccg ctgcgtgaag
    37861 cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat gaacgtcttt tgcgaagcag
    37921 agggccaggc gcaccgttta agctttaaat cgccgattct gctctactcc gatttcaaac
    37981 agctcacgac gatgaaagag gagcactacc gcgcagatac gctggatatc acctttgacg
    38041 tcactaaaac cacgctcgaa gcgacagtca aagagctgtg cgacaaagcc gaaaaaatgg
    38101 tacgtagcgg caccgtgctg ctggtgctct ccgaccggaa tatcgctaaa gatcgcctgc
    38161 cggttccagc cccgatggcg gttggcgcga tccagacccg tctggtcgat caaagcctgc
    38221 gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg cgatccgcac cacttcgccg
    38281 tgttgctggg cttcggcgcg acggctattt atccatacct tgcctatgaa acgctgggcc
    38341 gcctggtaga cacccatgcg attgccaaag attatcgtac cgtgatgctc aactaccgta
    38401 acggcatcaa caaaggcttg tacaaaatca tgtccaaaat gggcatctcc accatcgcct
    38461 cttaccgctg ctcgaaactg tttgaagcgg tcggtctgca cgatgatgta gtgggcctgt
    38521 gcttccaggg ggcggtcagc cgcattggtg gggcgagctt tgaagacttc cagcaggatc
    38581 tgctgaacct gtcgaaacgt gcctggctgg cgcgtaagcc catcagccag ggcggcctgc
    38641 tgaaatacgt ccacggcggc gaataccacg cttacaaccc ggacgtggtg cgcacgctgc
    38701 aacaggcggt acaaagcggc gagtacagcg actatcagga atacgcgaag ctggttaatg
    38761 agcgtccggc aaccacgctg cgcgatctgc tggcaattac gccgggtgaa aacgcggtca
    38821 acattgctga tgttgaaccg gcaagcgaac tgtttaaacg ctttgatacc gccgcgatgt
    38881 ctatcggcgc gttaagcccg gaagcccacg aggcgctggc ggaagcgatg aatagcatcg
    38941 gcggtaactc gaactccggt gaaggcggcg aagacccggc gcgctatggc actaacaaag
    39001 tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt tactccggcg tatctggtca
    39061 atgccgacgt cattcagatt aaagtcgccc agggcgcgaa gccgggcgaa ggcggtcagt
    39121 tgccgggcga caaagtcact ccttacatcg ccaaactgcg ctattcggtg cccggagtga
    39181 cgctgatctc cccgccgccg caccacgata tctactctat cgaggactta gcgcagctca
    39241 ttttcgacct caagcaggtt aacccgaaag cgatgatctc cgtgaagctg gtttccgaac
    39301 cgggcgtagg caccatcgcg actggcgtgg caaaagctta tgccgattta atcaccatcg
    39361 caggctatga cggtggcacc ggcgcaagcc cgctgtcgtc agtgaaatac gcaggttgcc
    39421 cgtgggagct ggggcttgtt gaaacccagc aggcgctggt tgccaatggt ctgcgtcata
    39481 agatccgttt gcaggtcgat ggcggcctga aaaccggtgt tgatatcatc aaagcggcga
    39541 ttctcggcgc agaaagcttc ggcttcggca ctggcccaat ggtcgcgctc ggctgtaaat
    39601 atctgcgtat ttgccacctg aacaactgcg caacgggtgt ggcaactcag gatgacaaac
    39661 tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac gaattacttt gagtttatcg
    39721 cccgtgaaac ccgcgagctg atggcacagc tgggggtaac gcggctggtg gatctgattg
    39781 gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc caaacagcag aaactggcgc
    39841 tgtcgaagct gctggagacc gccgaaccgc atccaggtaa ggcattctac tgcaccgaaa
    39901 acaacccgcc gtttgataac ggcctgctga acgcgcagtt gctgcaacag gcgaaaccgt
    39961 ttgtcgatga acgccagagc aaaaccttct ggttcgatat ccgcaatacc gatcgctccg
    40021 tgggcgcgtc gctttcaggc tatatcgccc agacgcacgg cgatcagggg ctggcagccg
    40081 atcctatcaa agcgtacttc aacggcaccg caggccagag tttcggtgta tggaacgcgg
    40141 gcggcgtgga actgtacctg accggggatg ccaacgacta tgtcggtaaa ggcatggcgg
    40201 gcggcttaat cgccattcgt cctccggttg gttccgcctt ccgcagccat gaagcaagta
    40261 ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg tctgtatgcc gcaggccgcg
    40321 cgggtgaacg tttcggcgtg cggaactccg gtgctatcac cgtggtagaa ggcattggcg
    40381 acaacggctg tgaatatatg acgggcggta ttgtctgtat tctgggtaaa accggcgtta
    40441 acttcggtgc gggcatgacc ggcggcttcg cttacgttct cgatgaaagc ggtgatttcc
    40501 gcaaacgcgt taacccggaa ctggtcgagg tcttaaacgt tgacgatctg gcgatccatg
    40561 aagagcatct gcgcggtctt atcaccgagc atgttcagca tactggctct cagcgcggtg
    40621 aagagattct ggcgaactgg tcaaccttcg ccactaaatt tgcgctggtt aaaccgaagt
    40681 ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag cgcagcagag ttgcgcgtgc
    40741 aggcgcagta aggggtagca acaatgagtc agaatgttta tcaatttatc gacctgcagc
    40801 gcgttgatcc gcctaagaaa ccgctgaaga tccgcaaaat tgagtttgtt gaaatttacg
    40861 agcagttttc cgaaggccag gccaaagcgc aggctgaccg ctgcctgtcg tgcggcaacc
    40921 catactgcga gtggaaatgt ccggtacaca actacatccc gaactggctg aagctcgcca
    40981 acgaggggcg tatttttgaa gcggcggaac tgtcgcacca gaccaacacc ctgccggaag
    41041 tttgcggacg agtctgcccg caagaccgtc tgtgcgaagg ttcctgcact ctgaacgatg
    41101 agtttggcgc ggtgaccatc ggcaacattg agcgttatat caatgataaa gcgttcgaaa
    41161 tgggctggcg tccggatatg tccggcgtga agcaaaccgg taaaaaagtg gcgattatcg
    41221 gcgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac gcgtaacggc gtaaaagccg
    41281 ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac cttcggtatt ccggccttca
    41341 agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt caccggcatg ggtattgaat
    41401 tcaaactcaa taccgaagtg ggccgcgacg tgcagctgga cgatctgctg agtgattacg
    41461 atgccgtctt ccttggcgtc gggacttatc agtcaatgcg cggcggtctg gaaaacaaag
    41521 acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc caacaccaaa cagttaatgg
    41581 gctttggtga aacccgcgac gaaccgttcg tcagcatgga aggcaaacgc gtggtggtcc
    41641 ttggcggtgg cgacactgcg atggactgcg tgcgtacgtc cgtgcgccag ggagcgaagc
    41701 acgttacctg tgcctatcgt cgtgatgaag agaacatgcc gggttcccgc cgcgaagtga
    41761 aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt ccagccactg gggattgaag
    41821 tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg taccgaaatg ggcgaaccgg
    41881 atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc cgaacatatc gttccggcag
    41941 atgcagtgat catggcgttt ggtttccgtc cacacaacat ggaatggctg gcaaaacaca
    42001 gcgtcgagct ggattcacaa ggccgcatca tcgccccgga aggcagcgac aacgccttcc
    42061 agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt ccgtggttcc gatctggtgg
    42121 taaccgctat tgccgaaggt cgtaaggcgg cagacggtat tatgaactgg ctggaagttt
    42181 aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc gctgaaattc tcctttcttt
    42241 ctggaaagcg cctcgcataa tctgaagaaa accaatgtaa tacgctcatc tggattgtta
    42301 ccggtagatg cccggcgtat agctgattcc attattcaac gcagcgccat ataaag
//
LOCUS       DADLTD010000007        42336 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLTD010000007 DADLTD010000000
VERSION     DADLTD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.007"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            100..639
                     /locus_tag="JN701_000288"
     CDS             100..639
                     /locus_tag="JN701_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362835.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQVAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(734..2311)
                     /gene="guaA"
                     /locus_tag="JN701_000289"
     CDS             complement(734..2311)
                     /gene="guaA"
                     /locus_tag="JN701_000289"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY7362836.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2380..3846)
                     /gene="guaB"
                     /locus_tag="JN701_000290"
     CDS             complement(2380..3846)
                     /gene="guaB"
                     /locus_tag="JN701_000290"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY7362837.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4008..5378
                     /gene="xseA"
                     /locus_tag="JN701_000291"
     CDS             4008..5378
                     /gene="xseA"
                     /locus_tag="JN701_000291"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY7362838.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5375..5590)
                     /locus_tag="JN701_000292"
     CDS             complement(5375..5590)
                     /locus_tag="JN701_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362839.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5659..7131)
                     /gene="der"
                     /locus_tag="JN701_000293"
     CDS             complement(5659..7131)
                     /gene="der"
                     /locus_tag="JN701_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY7362840.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7249..8427)
                     /gene="bamB"
                     /locus_tag="JN701_000294"
     CDS             complement(7249..8427)
                     /gene="bamB"
                     /locus_tag="JN701_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY7362841.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8438..9058)
                     /locus_tag="JN701_000295"
     CDS             complement(8438..9058)
                     /locus_tag="JN701_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY7362842.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9076..10350)
                     /gene="hisS"
                     /locus_tag="JN701_000296"
     CDS             complement(9076..10350)
                     /gene="hisS"
                     /locus_tag="JN701_000296"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY7362843.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10461..11579)
                     /gene="ispG"
                     /locus_tag="JN701_000297"
                     /gene_synonym="gcpE"
     CDS             complement(10461..11579)
                     /gene="ispG"
                     /locus_tag="JN701_000297"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY7362844.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11606..12619)
                     /gene="rodZ"
                     /locus_tag="JN701_000298"
     CDS             complement(11606..12619)
                     /gene="rodZ"
                     /locus_tag="JN701_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY7362845.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12904..14058)
                     /gene="trmG/rlmN"
                     /locus_tag="JN701_000299"
     CDS             complement(12904..14058)
                     /gene="trmG/rlmN"
                     /locus_tag="JN701_000299"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY7362846.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14208..14639)
                     /gene="ndk"
                     /locus_tag="JN701_000300"
     CDS             complement(14208..14639)
                     /gene="ndk"
                     /locus_tag="JN701_000300"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY7362847.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14788..17100)
                     /gene="pbpC"
                     /locus_tag="JN701_000301"
     CDS             complement(14788..17100)
                     /gene="pbpC"
                     /locus_tag="JN701_000301"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAY7362848.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17101..22062)
                     /gene="yfhM"
                     /locus_tag="JN701_000302"
     CDS             complement(17101..22062)
                     /gene="yfhM"
                     /locus_tag="JN701_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HAY7362849.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPANALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPSDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPLVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGDEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22269..23114
                     /gene="sseA"
                     /locus_tag="JN701_000303"
     CDS             22269..23114
                     /gene="sseA"
                     /locus_tag="JN701_000303"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY7362850.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(23607..24383)
                     /gene="sseB"
                     /locus_tag="JN701_000304"
     CDS             complement(23607..24383)
                     /gene="sseB"
                     /locus_tag="JN701_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY7362851.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24525..25808)
                     /gene="pepB"
                     /locus_tag="JN701_000305"
     CDS             complement(24525..25808)
                     /gene="pepB"
                     /locus_tag="JN701_000305"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY7362852.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(25986..26186)
                     /gene="iscX"
                     /locus_tag="JN701_000306"
     CDS             complement(25986..26186)
                     /gene="iscX"
                     /locus_tag="JN701_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY7362853.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26198..26533)
                     /gene="fdx"
                     /locus_tag="JN701_000307"
     CDS             complement(26198..26533)
                     /gene="fdx"
                     /locus_tag="JN701_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY7362854.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26535..28385)
                     /gene="hscA"
                     /locus_tag="JN701_000308"
     CDS             complement(26535..28385)
                     /gene="hscA"
                     /locus_tag="JN701_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY7362855.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28402..28917)
                     /gene="hscB"
                     /locus_tag="JN701_000309"
     CDS             complement(28402..28917)
                     /gene="hscB"
                     /locus_tag="JN701_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY7362856.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29013..29336)
                     /gene="iscA"
                     /locus_tag="JN701_000310"
     CDS             complement(29013..29336)
                     /gene="iscA"
                     /locus_tag="JN701_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY7362857.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29353..29739)
                     /gene="iscU"
                     /locus_tag="JN701_000311"
     CDS             complement(29353..29739)
                     /gene="iscU"
                     /locus_tag="JN701_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY7362858.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(29767..30981)
                     /gene="iscS"
                     /locus_tag="JN701_000312"
     CDS             complement(29767..30981)
                     /gene="iscS"
                     /locus_tag="JN701_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY7362859.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31093..31581)
                     /gene="iscR"
                     /locus_tag="JN701_000313"
     CDS             complement(31093..31581)
                     /gene="iscR"
                     /locus_tag="JN701_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY7362860.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(31851..32591)
                     /gene="trmJ"
                     /locus_tag="JN701_000314"
     CDS             complement(31851..32591)
                     /gene="trmJ"
                     /locus_tag="JN701_000314"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY7362861.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            32710..33513
                     /gene="suhB"
                     /locus_tag="JN701_000315"
     CDS             32710..33513
                     /gene="suhB"
                     /locus_tag="JN701_000315"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY7362862.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            33658..34512
                     /locus_tag="JN701_000316"
     CDS             33658..34512
                     /locus_tag="JN701_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7362863.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            34703..35983
                     /gene="csiE"
                     /locus_tag="JN701_000317"
     CDS             34703..35983
                     /gene="csiE"
                     /locus_tag="JN701_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY7362864.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(35975..37114)
                     /locus_tag="JN701_000318"
     CDS             complement(35975..37114)
                     /locus_tag="JN701_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY7362865.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37274..38164)
                     /gene="hcaR"
                     /locus_tag="JN701_000319"
     CDS             complement(37274..38164)
                     /gene="hcaR"
                     /locus_tag="JN701_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY7362866.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38300..39661
                     /locus_tag="JN701_000320"
     CDS             38300..39661
                     /locus_tag="JN701_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HAY7362867.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            39658..40176
                     /locus_tag="JN701_000321"
     CDS             39658..40176
                     /locus_tag="JN701_000321"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY7362868.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40176..40496
                     /locus_tag="JN701_000322"
     CDS             40176..40496
                     /locus_tag="JN701_000322"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY7362869.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            40493..41305
                     /locus_tag="JN701_000323"
     CDS             40493..41305
                     /locus_tag="JN701_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HAY7362870.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41315..>42336
                     /locus_tag="JN701_000324"
     CDS             41315..>42336
                     /locus_tag="JN701_000324"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY7362871.1"
                     /translation="MKEKTIIIVGGVQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPWFWSDQYSDNLQFI
                     GDMRGD"
BASE COUNT        10005 a        11708 c        10630 g         9993 t
ORIGIN      
        1 acgacaggtt tagccgttgt tattaccaca acgtataacg aaacgcgtat tcagccaaat
       61 actaaatgtc ctgaaaagag ctaataatca ggaggagtca tgaagaaagt ttttctttgc
      121 gccatcttgg cctccttaag ctatccggct atcgcctcat cattgcagga tcaactctct
      181 gctgtagcag aagcggaaca gcaaggtaaa aatgaagagc aaaggcagca tgacgaatgg
      241 gtcgcggagc gcaacaggga aatccagcaa gagaagcaac gtcgcgcaaa tgcccaggtc
      301 gccgctaaca aaagagcggc aacggcagca gcaaataaga aagctcgtca ggataaactg
      361 gacgccgaag cctctgcgga caaaaaacgc gatcaaagtt atgaagatga gctacgcagc
      421 ttagagattc agaaacaaaa actggcgctg gcaaaagaag aagcccgcgt taagcgagaa
      481 aacgaattta tcgatcagga actgaagcac aaagctgcgc aaaccgatgt ggtgcaatct
      541 gaagctgacg ccaacagaaa tatgactgaa ggcggtcgcg atctgatgaa aagcgtgggc
      601 aaagcagaag agaacaaatc ggacagctgg tttaattaat cgatgttagt aacttcaagc
      661 ctatgattct tgaagataaa aaaccctctg tagtaacaga gggttttgtt cattcatagt
      721 gcagggatta aaatcattcc cactcaatgg tagctggcgg cttgccgctg atgtcataca
      781 ccacgcggga aataccgttc acttcattga taatgcggtt ggaaacgcga ccgaggaaat
      841 cgtacggcag atgcgcccag tgtgcggtca taaagtcgat ggtttcgaca gcacgcagag
      901 agacaaccca gtcatactta cgaccatcgc ccattacgcc aacggaacgt accggcagga
      961 acacagtgaa cgcctggctg actttgttgt acaggtccgc tttacgcagt tcttcaatga
     1021 agatggcgtc agcacggcgc agcaggtcac agtactcttt cttcacttca cccagcacac
     1081 gaacgccaag gcctggtccc gggaacgggt gacggtacag catgtcgtac ggcaggccca
     1141 gctccagacc aatcttacgc acttcgtctt tgaacagctc tttcagcggt tcaaccaggc
     1201 ccatcttcat ctctttcggc aggccgccca cgttgtggtg agatttgatg acgtgtgctt
     1261 taccggttgc agaagccgca gattcgataa cgtcagggta gatggtgccc tgcgccagcc
     1321 acttcacgtc ttccagtttc agcgcttctt catcgaacac ttcaacgaaa acgcgcccga
     1381 tgattttacg ttttgcttcc ggatcgtttt cgccagccag cgctgacagg aagcgatctt
     1441 ctgccggtac gtgaacaatg ttcagaccaa agtgatcgcc aaacatatcc agaacctgct
     1501 ctgcttcgtt gagacgcagc aggccgttgt cgacgaatac gcaagtcaga tttttaccga
     1561 tagcgcggtg cagcagcatt gcggttacgg aggaatccac accaccagag aggccgagga
     1621 tgactttatc gtcgcctacc tgctcgcgga tgcgagctac agcatcgtcg ataattttcg
     1681 ctggcgtcca cagggcttca cactggcaga tatcacgcac aaaacgctcc agcatgtgca
     1741 taccctggcg ggtgtgagtc acttccgggt ggaactgtac gccatagaag cgtttttctt
     1801 cgttagccat aatggcaaac gggcagcttt cggtgctggc tacggtgacg aagtcggacg
     1861 ggatagcggt aactttatcg ccgtggctca tccagacatc gagcagcggt ttaccgtctg
     1921 cggtcagcgc atcttcgata ccgcgaacca gtgcgctgtc gtttacgact tcaacctgcg
     1981 cgtagccaaa ttcacgttcg ttagaggctt caacgtgacc gcccaactgc attgccatgg
     2041 tctgcatgcc atagcaaacg ccgaataccg gtacgcctgc ttcaaagaca tactgcggcg
     2101 cacgcggact gttttcttca gtagtacttt ccgggccgcc ggaaagaata atgccgcttg
     2161 gattgaagtc acgaatttgt gcttctgtca catcccacgc ccacagttcg cagtaaacac
     2221 ccagctcacg cacgcggcgc gcaaccagtt gagtgtactg agaaccgaag tccagaatga
     2281 ggatgcgatg cttatgaatg ttttccgtca ttgacgctta ttccgaggca agtgaaacag
     2341 ataatataaa tcgcccgaca tgaagtcggg cgaagagaat caggagccca gacggtagtt
     2401 cggggactct ttagtaatgg tcacgtcgtg aacgtggctt tcctgaatgc ccgcaccgct
     2461 gatacgtaca aactccgctt tagtacgcag ttcgtcgata gtaccacagc cggtcaggcc
     2521 catacaggag cgcaggccgc ccatctgctg gtgaatgatc tctttcaggc gacctttata
     2581 ggctacgcga ccttcgatac cttccggcac cagtttgtcg gcagcgttgt cactctggaa
     2641 gtaacggtca gaagaacctt tggacatcgc acccagagag cccataccac ggtaagattt
     2701 gtaagaacgg ccctggtaga gttcaatttc acccggagat tcttcagtac ccgccagcat
     2761 ggaacccacc attaccgcgc ttgcgccagc ggcgatagct ttggcgatgt cgccggagaa
     2821 gcgaatacca ccatctgcga taaccggaat acctgtgcct tccagcgctt caactgcgtc
     2881 agcaacggca gtgatctgcg gaacacctac gccagtaacg atacgagtcg tacagataga
     2941 accagggccg ataccgactt taaccgcgct gcaaccagct tctgccaaag cgcgtgcacc
     3001 tgcagctgtt gccacgttgc cgccgatgat ttgcagatcc ggatatttag cacgggtttc
     3061 acggatacgc tgcaaaacgc cttctgagtg gccgtgggag gagtcgatca gcagaacgtc
     3121 aacgccagcg gcaaccagcg cgtcaacacg ctcttcgtta cccgcacctg cgccaactgc
     3181 agcaccaaca cgcagacggc cttgctcgtc tttacaggcg ttcggtttac gttccgcttt
     3241 ctggaagtct ttcacggtga tcatgccgat caggtggaat tcgtcatcaa ccaccagcgc
     3301 tttttcaacg cgtttttcgt gcatttttgc cagcaccact tcacgggctt caccttcacg
     3361 cacggtgacc agacgctctt tcggcgtcat gtaaacgcta accggctggt tcaggtcggt
     3421 aacaaaacgc acgtcacgac cggtgataat acccaccagt tcgttttctt cggtaacaac
     3481 cggatagccc gcaaaaccgt tacgctcggt cagttctttc acttcgcgca gcgtcgtggt
     3541 tggcagaaca gtctgcggat cagtcaccac accagattcg tgttttttca cacggcgaac
     3601 ttcttctgcc tggcgttcaa tggacatgtt tttgtggata aagccgatac cgccttcctg
     3661 agccagagca atagccaggc gcgcttccgt tacggtatcc attgctgcgg aaagcatagg
     3721 gatattcaga cgaatagttt tcgtcagctg ggtgctgagg tcagcagtat tcggcagaac
     3781 ggtagaatga gcaggaacga ggagaacgtc gtcaaacgtc agagcttctt tagcgatacg
     3841 tagcatgggc aatatctcga ccagagtggt taataaatat tgccgcggca ttatacagag
     3901 cgtaaccgat tgcatctacc cctttttgca aaaaatgctt gctatccccg aagggcgggt
     3961 tactatcgac tgaataacct gctgatttag aatttgatct cgctcacatg ttaccttctc
     4021 aatcccctgc aatttttacc gttagtcgcc tgaatcaaac ggttcgtctg ctgcttgagc
     4081 atgagatggg acaggtttgg atcagcggcg aaatctctaa tttcacacaa ccggcttccg
     4141 gtcactggta ctttacactc aaagacgaca ccgcccaggt acgctgcgcg atgttccgca
     4201 acagcaaccg ccgggtgacc ttccgtccac agcatggaca acaagtttta gttcgcgcca
     4261 atattacgct ctacgagccg cgcggtgact accagataat cgttgagagt atgcagccgg
     4321 ccggtgaagg gctgctgcaa cagaagtacg aacagctcaa agcgaagttg caggctgaag
     4381 gtttgttcga tcagcaatac aaaaaaccac ttccctcccc tgcgcattgc gttggtgtga
     4441 tcacctcaaa aaccggtgct gcgctacatg atattttgca tgtgttaaaa cgtcgcgatc
     4501 cttctctacc ggtgatcatc taccccaccg ccgttcaggg cgatgacgca ccggggcaaa
     4561 ttgttcgcgc cattgaactg gcgaatcagc gcaatgagtg cgacgtgttg atcgttgggc
     4621 gcggcggcgg ttcgctggaa gatttatgga gttttaacga cgaacgcgta gcgcgggcga
     4681 tttttgccag ccgcattccg gtcgtcagcg ccgtcgggca tgagacggat gtgaccattg
     4741 ccgattttgt tgccgatctg cgtgcgccaa cgccgtctgc cgccgctgaa gtagtgagcc
     4801 gtaatcagca agagttactg cgccaggtgc aatcgacccg tcaacggctg gagatggcga
     4861 tggattatta tctcgccaac cgcacgcgtc gctttacgca gatccatcac cgattacagc
     4921 aacagcatcc gcagctccgg ctggcacgcc agcaaaccat gcttgagcgc ctgcaaaagc
     4981 ggatgagctt tgcgctggaa aatcaactta agcgtaccgg gcaacagcag cagcggttaa
     5041 cacagcggct gaatcagcaa aatccacagc cgaagattca tcgcgcgcaa acgcgcattc
     5101 agcaactgga atatcgttta gcagaaaccc tgcgcgtaca gcttagcgcc acgcgtgaac
     5161 gtttcggtaa tgcagtaacg cacctcgaag ccgtaagccc actgtcaacg ctggcgcgtg
     5221 gatacagcgt tactactgct actgacggca atgtactgaa aaaagtgaag caagttaaag
     5281 cgggtgaaat gctaaccaca cgtctggaag acggctggat agaaagtgaa gttaaaaaca
     5341 tccagccagt aaaaaaatcg cgtaaaaagg tgcattaagc cagcacgaat tcaacgcgtt
     5401 ttttcgatat caagccatgc ccgtgctgac agaagtaatc tactgcaccg caggctttta
     5461 acacctgcaa cggttgatgg cagtctggac aaagagcttt catttcaata atttccccgc
     5521 aggatgggca acgcgcatgg ccgttatcct gatcaagcac atgctgacat tgtggacaat
     5581 gcagttccat atgaattcct ccggtagcca tccctctaca ttcatagagg gaatggcaga
     5641 taaaatactt acggataatt atttattttt cttgatgtgc ttcatcagac gcttacgttt
     5701 acgcatctgg gttggcgtca gggtgttacg cttgttcgca tacgggtttt ccccttcttt
     5761 gaactgaata cgaatcggcg atcccattac gtccagcgat ttgcggaagt agttcatcaa
     5821 gtagcgcttg taggaatcag gcaggtcttt cacctgatta ccgtgaatca ccacaatcgg
     5881 cgggttataa ccaccagcgt gggcatattt cagcttcaca cgacgaccgc gtaccagcgg
     5941 cggttggtga tcttcaacag ccatcgtcat gatgcgcgtc agcatagagg tccccacacg
     6001 acgggtggag ctgtcatacg cttcacgtac tgattcaaac aagttaccaa caccactgcc
     6061 gtgcaaggca gagataaagt gcacacgagc aaaatcgata aagcccagac ggaagtccag
     6121 cgtttctttc acctgctctt tcacttcctg gctcaggcca tcccacttat tcaccacaat
     6181 gacaagtgag cgcccactat tgagaataaa gcccagcagc gagagatcct gatcggaaat
     6241 accttcgcgc gcatcaatca ctaacatcac cacgttggca tcttcaatgg cctgcaacgt
     6301 tttgattacg gagaatttct ctacagcatc ggtgattttg ccgcgtttac gtacgccagc
     6361 ggtgtcaatg agcacatact cacgtccatc gcgttccatt ggaatgtaga tactgtcacg
     6421 cgtcgtgcca ggcatgtcat aaacaacaac gcgctcttca ccaagaatac ggttagtgag
     6481 tgtagactta cctacgttcg gacgacccac aatcgccagt ttgatcggca gactttgcgg
     6541 gtcgaagtcg tcttcctctt cttcttcgcc gttctcttcc acttcaaatt gcgcccagta
     6601 ttcagcgtct tcgtcgactt cctcttgcgg tgcgagatct tccatccacg gcagcagcac
     6661 atgctccagc agacttaata cgccacgacc gtgagacgcg gcgatcgggt agatttcacc
     6721 taagccaagc gagtagaaat caaccactgc ctgatcggga tccagaccgt cagttttgtt
     6781 tgccaccagg aaggtcggtt tttcacggga gcgcagatgt ttggcaatcg cttcatctgc
     6841 cggcatcagg cccgcgcgcg catccaccat aaacagtacg acgtccgctt cttcaatcgc
     6901 cagcagcgac tgttccgcca tgcgggtttc tacaccgtct tctgtgccat caatcccgcc
     6961 ggtatcaata cagataaact cacggccttc aatttccgca cgaccgtact tacggtcacg
     7021 agtcagaccc gggaaatccg caaccagcgc atctcgggtg cgagttagac ggttaaataa
     7081 cgtggatttt cctacgttag ggcgcccgac aagcgcgacc acaggtacca tgtttaaagc
     7141 ctcattttta taaatcatca gacaacgcac gctatattcg cgtcgttgtt aaaaacagga
     7201 aaacggcccc tgtccaggag ccgttttcaa agtgaacgac agagacgatt aacgtgtaat
     7261 agagtacacg gttccgtctt ttgcctggat cagcagtttg ccgtcagcgg caaccggttc
     7321 agtctggaaa ccggaactat caactttttg ctgggcaacg aaacgaccat cttcgacatt
     7381 aatccagtgc agataacctt cactgtcacc gaccaccagg ttgccatcat acagcaccgg
     7441 agaagtcagc aggcgatgca acagatcgct ttgtgtccac agcgtaacgc cgccatcaat
     7501 ggtcaacgcc atcacccggt cattttgatc gaccagatag atgcgattgc cgtcgacgat
     7561 gaaatcattc accgaaccca gttcgcgttt ccacataatc tgaccactgc gcagatcaag
     7621 cgccgtcagg ttaccattat aggccagcgc gaaaacaacg ccgttaacaa cgacgggagt
     7681 cgtgtcaaca tcgctcagac ggtcaatttc ggtagaaccg gtcgcctggg aaatacgctg
     7741 ctgccaaatc atctggccct gttccatcag cactgcgctg acgcgaccat tatcgccccc
     7801 cacgacggcc gcaccaaaag ccgttgccgg cgcagactcg ccacgcaaag agagcgaagg
     7861 catatcgagg ttaactgtcc atttgacagc gccgtcagct tcgttcagcg cttgtaactg
     7921 accgttactg gtgtggatta acaccagacc gtcgctgacc accgggcgcg aaagtgcttc
     7981 acccgcgact ttagtttgcc atgccacagt accatcgctg gtattcagcg cgtaaacctg
     8041 cgccttttcg ctgccaatgt agacatgccc accagacacg gtcacaccgc cagaaagtaa
     8101 tgcaggctct ttagagaacc agccatcttt ctcggccagg ctgacagacc agatttcttt
     8161 gccatcatcc gcattcagcg cttttaccaa accagcgcgg tccgctgcat agacaacgtt
     8221 gtccgccagt gccggatgaa gattggaata gaagttgcca atgccgctac caacggaagt
     8281 actccacgcc gtggtcggcg taaactggtt ttcaacggtt ggcaatgggg acatctttac
     8341 cacatcttct tcgctgttaa acagcgaaca gccgcttaaa agggtaacgg aaagcagtcc
     8401 tggcagcagt aatttacgca attgcatcgg gtccctctca gatggacaaa ttattaattt
     8461 tcatctgcat catttcgctc agtgccggag taacatcgct tttcacgcct gcttcccatg
     8521 cactacgcgc accttgctta tcacctttgc tcagcaatgc ttcaccacgc aggtcggcaa
     8581 caatggcagc ccacccttca cctttgatgg tatcaagggt tttcagcgcg gcatcagcct
     8641 gcttgagctg tacctgaacg cgagcaagac gcagatttat cacggctttg agattttcat
     8701 cgctcgtgtc tgccagcccc tgttgtaact gggcggcagc tttctctagt tcatttttgt
     8761 caacaaattg ctgcgcaagt tccaaagaag ccagcgcacc ataagtattt ttattttcag
     8821 cagcaaattt ttccgccgcc gggatgctat ccggtttgcc ttcgctcact gcggtaaccg
     8881 cattttgata ggcaagagaa gcggagcgtg cagaatcaac ctgatggctg ttccagtagc
     8941 gccagccaat cagtgcgcca acgcccaaaa tcaccccaac agccagtgct ttgccatttt
     9001 cagcaaaaaa gcgtttaacc gcttctacct ggtcgttttc gttctcgtaa atttccacgc
     9061 tgtccttctc cttccttaac ccagtaacgt gcgcaaatgc gcggctacgc tatcctgcgc
     9121 aactgccgtt tgctcaccag agcgcaaatc cttcactact gctgtgccgt tagtcacttc
     9181 agactcaccc agcaccacag caacgcgggc accccattta tcagcacggg caaactgttt
     9241 cttaaagttg ccgccgccgt ggttggtcat caatttcacg ccctgtaatt catcacgcag
     9301 acgctcagct aatgccatag ccgcagattg tgtatcagca cctgaagcca ccaggtatat
     9361 atcgacaata ggatcggctt taaattccgg attaacggcc tgtactaaca atacaagacg
     9421 ttcgaggccc atcgcaaaac cgacagccgg tgttgcacga ccgcccagtt gttccacaag
     9481 accgtcataa cgaccgcctg cacacacggt gccctgggag ccgagactgt tagtcaccca
     9541 ctcgaaaacg gtacggttat agtaatccag accacgcacc agacgctggt ttacggtgta
     9601 agcgatcccc gcgctctcaa gcagtttgca cagaccggca aaatgctcac gagattcctc
     9661 gtccagataa tcacctaatg ccggagcgtc gttgagaagc gcctgcactt ccggattttt
     9721 tgaatccagc acgcgcagcg ggttagtgta catgcggcgt ttgcagtctt cgtccagctt
     9781 ttctttatgc tgctcaagga atgccaccag cgcatcgcgg taattggcgc gtgcttccag
     9841 cgaaccgata gaattcagct caagagttac gtgttcggaa atacccagcg cgcgccacca
     9901 gcgggcggtg agcataatca gttcagcgtc gatatccgga ccttgcagac cgaaaacttc
     9961 gcagcccaac tgatggaact gacgataacg ccctttctgc ggacgctcgt gacggaacat
    10021 cggcccgata taccacagac gctgttcctg attgtacaga agaccatgct cgatgccggc
    10081 gcgtacacag cccgccgtcc cttcagggcg cagagtcagg ctgtcgccat tgagatcctc
    10141 aaaggtgtac atctcttttt caaccacgtc ggtgacttca ccaatcgcac gtttgaatag
    10201 cggggtctgc tctacaatcg gcaagcggat ttcactgtaa ccgtagctgc cgagcacgtt
    10261 tttcagtgtg ccttcaatgc gctgccagat ggccgtttcg ccaggcaggt aatcgttcat
    10321 gccgcgaatg gcttgaatgt tttttgccac gtttattctc tttctgaata taaaaatgaa
    10381 ccctcaacgc ttccctcaat gtttggggag ccatgcgggt tcaatcatac acgggaagcg
    10441 aggcgcttcc catcacgtta ttatttttca acctgctgaa cgtcaattcg acgcgcttca
    10501 tccagctgac tggctttcgc acgaatgcgt gcttccagct ggtcgatcat atcgttgttg
    10561 tccagacggt ctttgcgcac gccatcttca tagaggccgc ttttcttgtt gccgccggtg
    10621 acgccgagtg tagaaaccag cgcctcacct gggccattca ccacgcagcc gataatagaa
    10681 acgtccatcg gagtgatgat atcttccagg cgttgctcca gcgcgttaac cgtaccgata
    10741 acatcaaatt cctgacgcga acaggtcggg caggcgatga agttgatccc tcgcgaacgg
    10801 atacgcagcg atttcaaaat atcgaaaccg actttgatct cttcgaccgg atcggccgcc
    10861 agcgatacgc gcagcgtgtc gccgatgcct tcagacagca gcagacctaa accaatggcg
    10921 gattttactg ccccgctgcg tgcaccaccg gcttcggtga tccccagatg cagcggctga
    10981 tcgatctgtt ttgccagcaa acgataagac tcaacagcga ggaagacgtc agacgctttc
    11041 acgctgactt tgaactgatc gaagttcagg cgatcgagat gatcaacatg acgcatggcg
    11101 gattccagca acgcctgcgg cgtcggttcg ccatactttt cttgcagatc tttttccagc
    11161 gatccggcgt taacgccaat acggatcgga atgtttttat cgcgcgcaca gtcaaccacc
    11221 atgcgaatac gctcttcatt accgatattg ccagggttaa tacgcagaca atcgacgccg
    11281 tattccgcta ctttcagcgc aatgcgatag tcgaagtgga tgtcagccac cagcggcacg
    11341 ttaacccgct gtttgatgag tttgaacgct tctgccgcgt ccatcgtcgg tacggagaca
    11401 cggacgatat cagcgccaac gcgttccagc gccttgattt gattgaccgt tgcttcgaca
    11461 tctgtcgtac gcgtattggt catggactgt acggcgatgg gagcaccatc gccaatcggc
    11521 acattcccaa cgtaaatacg tgttgatttt ctacgttgaa ttggagcctg gttatgcatg
    11581 aaaaatctcc cgcgttaccc gtctgttact gcgccggtga ttgttcggca ttgagggtca
    11641 gacgcgcaac ctggttagtt ctgataaaac gactcagatc gacaggtttc ccttgatact
    11701 ggatctgtac tgcggctggc gcaccaattt tcagtttgta cggtgcctgg ccggttaagt
    11761 tcaaattacc gtctttacgc tgcataccgc taaacaattt tttaccggta gcatcagtga
    11821 cctccagcca gcaatcggca gtaaagttca tcaccagcgc attcggatca gccaccggcg
    11881 tggtcacgcc agcctgatcg gttggcaacg gcgcagcacc atctggcgtt gttgctgccg
    11941 ttggtgccgg ggtcgcggcg gtatcaacat ttgcctgcga aggcgaaaca accgcattct
    12001 gttgcggatc aacagctggt gctggcgcag ttacggcagg tgtttgcgtg ttggttgcgg
    12061 tagtatccac agacgctggc ggcgtggacg ttgtagccgg gtctgtagtt gtcgacgtat
    12121 ttaacggaac actctgcccc tgctcgctat tactgctcag ttccgccgaa gattgatcgg
    12181 ccatagtggt gatctcttcc tgctgagctt tgtggtcttg ccaccaccag gcaccgctca
    12241 ggccgataac cacaaacaac accagccagg tgaaggtcat cagccagccg tcacgttttt
    12301 tgcggcgttt accgagggaa aaactctgca tcggcgcaac tttcgcagcc cgtagtggag
    12361 cctgcttttc cagccctagc agcagttctt cttctggaat atgtaccaga cgcgcataag
    12421 agcggatata tccgcgcagg aatgttgaag caagatcggc gggtgcctta tcttcttcaa
    12481 tgtcgcgtac cgtggaaacc ttcaggcaaa gtcgctcggc aacggcctgc tgactaagtc
    12541 ctagttgttc gcgagcatta cgcaggcgag cgccggtagt aagtgcttca ttttggtcgt
    12601 gcgtggcttc agtattcatt cgctgcaggt acgtttaaat gagaattgag atgccggtga
    12661 accatcatgc tcacccacac cgcgaaacat ccgttaagtt aaccgttatc atacagtata
    12721 agaccgtctg tgtgctcgtg acaaagccca cattcactaa ggctaactta ctgttgcatc
    12781 gttacatact gccttaaagt cagcaaaaac gcaccgttaa tattgaccag acaattgcaa
    12841 cttaatactt cattcataaa agattacgcc acggtacata aagtaaccgt ggcgtaatgg
    12901 ctatcagacc gctttaatgt cgatggcttc accctgcatc cgtttacgca gggtacgttt
    12961 cgtacggtcg ataacatcgc ccgccaactg accacaggca gcatcgatat catcaccacg
    13021 agttttacgc acaatagtgg tgaaaccgta gctcatcagc acttttgaga aacggtcgat
    13081 acggctgttc gaactgcgtc catacggcgc gcccgggaac gggttccacg ggatcaggtt
    13141 gatcttacac ggcgtatctt tcagcagctc cgccagttgg tgcgcgtgtt cagtgccgtc
    13201 gttaacgtgg tcaagcatca cgtattcaat agtgactcgg ccctgattgg cgttggattt
    13261 ctccagataa cggcgcaccg cagcaaggaa cgtttcgata ttgtactttt tgttgatcgg
    13321 cacaatttcg tcacgaattt cgtcgttcgg cgcgtgcagg gaaattgcca gtgcaacgtc
    13381 gatcatatcg cccagtttat ccagcgccgg aactacaccg gaagtagaaa gcgtgacgcg
    13441 acgtttagac aggccaaaac cgaaatcatc cagcatgatt tccatcgccg gaacgacgtt
    13501 gttcaggttg agcagcggct cgcccatgcc catcatcact acgttagtga ttgggcgctg
    13561 accggtgact tttgctgcgc cgacgatttt cgccgcacgc cacacctggc cgataatttc
    13621 cgacacccgc aggttgcgat taaagccctg ctgggcggtg gaacagaatt tacactccag
    13681 cgcacacccc acctgcgaag agacgcagag cgtggcacgg tcgtcttccg ggatatacac
    13741 cgtttcgacg cgctgatcgc caacggcgat cgcccattta atggtgccgt cggatgaacg
    13801 ctgttcttca accacttccg gtgcgcggat ttccgccacc tctttcagtt tgccgcgcaa
    13861 cactttgttg atgtcggtca tctcatcaaa gttgtcgcag caatagtgat acatccactt
    13921 catcacctga tcggcgcgga agggtttttc acctaaatct ttaaaaaact cccgcatctg
    13981 ctgacggttg agatccagca ggttgatttt tccatctttc gtggtgacgt tttcaggtgt
    14041 gactaattgt tcagacatat gctattccgg cctcgttatt acacgttatg gcccctggag
    14101 ggttgaaaaa agaaacgccc cggtgagcgg ctgctcgtcc gggggcgctg cattgtacaa
    14161 attctggcgc acggatgcca cgtttgcacg cggcatttac gaaattatta acgggtgcgc
    14221 gggcacactt cgccttcgcc aaagaaataa gcgatttcgc gagcggcaga ttcgacggaa
    14281 tcagaaccgt gggtaccgtt ttcggtcagg ctgtcagcgt aatcagcgcg cagagtacca
    14341 gccagtgcgt ttgccggatt ggtcgcgccc agcagatcgc ggtgacgctg aacggcgttt
    14401 tcaccttcca gcacggaaac cacgatcggg ccagaggtca tgaattcaac cagaccatca
    14461 aagaacggtt ttccatcgtg ttcagcataa aagccacgtg cctgttcaac ggtcaggtgc
    14521 agcattttgg tgccgacaat tttgaaccct gcagcttcaa agcgcgcaaa gatattacca
    14581 atgacgtttt ttgctaccgc gttcggtttg atgatggaaa aagtacgttc aatagccatt
    14641 tttacctctg taaattgttc tgttgttgtc tgtaccagcg tacgagatgg cgcggattat
    14701 aatgagcaac agggccgttg actattgatg aaggtaacat ttttttaaaa taaaatgagt
    14761 tttagcaaca atcagggaca aaacagacta ttgcatgaca aatttcactg tcgcgatttg
    14821 ccccacgtca tccatcacca gcaattgata atcaccttta tccgtcaaat gcagggtcac
    14881 gttgcgcccg cgttcagtta acggttcgcc attcagaaac caccagcgtt cacctgcccc
    14941 tccacttgat tgcaacggca aagtcgcttc cgctgcgccc ggtaaacgtt taataatcgc
    15001 gccatcgcga acgcctgtca gctgcagcgg gagctgggca tagtgaccgt atggcgggca
    15061 acttgtcgag gccggtggta agcgcacagc gcgacgttct gatgcgggca gccagggttc
    15121 cagcggtagc ggccagacat ttatcatttc ttgtcgcgct tgcgggcaat cagcggcaac
    15181 acgtttgcca ttttcatcca gccagatggg gaaacgaatg ccattaatgc cttcctgctc
    15241 cggcagtaat agagttggcg gctgactccc gtccagcagc caggttgcca ggcggcggcg
    15301 acagttaccg tcaccttccg gcaaagactg tccgcccggc cagcagataa cgccacgagt
    15361 gactgagttc gggcgcgggt cttccggcag attcgcactg cgcgacagta agatattatt
    15421 gacctgattc aacaatggta cggcactggc aaagccaaac tgaccaacaa cgggcgtgcc
    15481 gtccggtctg ccagtccaga tcccaatgac atagcgagcg ttaaccccaa tcgcccaggc
    15541 gtcacgatag ccatagctgg tgcccgtttt ccatgccagt ggggcgacgc gcggcaaggc
    15601 actatccggc aagggttgcg cttcatcagc cataatccgg cgaatgatcc acgccgcccc
    15661 cgacgacatt aaaggccatt caagcagcgg atcgtcaggc tgtaagcgca atttgcctgc
    15721 cttgccgtgg cgagcaaacg cggtatacgc tgccgccata tcttccagtt ttgcaccagc
    15781 gccgccgaga atgagtgaaa gattcggcgc agcaccgttg ggcaaatata acggcaatcc
    15841 aacattgcgt aactttgccg caaaccgttt cggtccatag gcttccagca cctgcacagc
    15901 aggtaagttc agcgagcgca ccagcgcctc gctcatgctg atcgggccat gaaaaccgct
    15961 atcaaagtta cctggtcgat aatcaccggt gcgccggggg acgtcttgca gcagtgatgc
    16021 cgggtggatc aagccttcat ccagcgccag accataaaca aacggtttga gcaccgatcc
    16081 tggcgatcgg atcgcattga ccatatcaac atgaccaaag cgtgaatcat cgttgagatc
    16141 aaccgatccc acccagccgc gaacacgcat atcggtatga tcaaccacga tcatcgccag
    16201 tgagctgcgc ggtggcaacc gccctttcca gttttgcgcc agttcttcca gacgtcgttg
    16261 aagaccggca tccagcgtag taacgatttt gtcgcttttg cttttaccga gcatcatgcg
    16321 cgaaaacagc ggtgccagtt gcggcatttg tcggggtgcc agccagatgg gttcttccct
    16381 tgactctttt acctgctcac gggaccacac accttgcacg gccatccgtt cgagcacttt
    16441 attacgcgcg gcttcggcac gctccggcca acgatccggg cgcagtcggc tgggcgcttg
    16501 cggtaacacc gccagcattg ccgcctcgga atagcttaaa ttcgcaggcg attttccgag
    16561 ataagcccaa cttgccgcac cgatcccctg caacgtaccg ccaaacggag cgcggttaag
    16621 atacaagatc agaatttcac gcttagacaa atgccactcc agttgcaacg cgcgccagag
    16681 ctggagaatt ttgccaccaa atgttttggg gtgaggatca agcagacgag caacctgcat
    16741 agtgagcgtg ctgccaccag aaataacccg tcccgaagtg agatcttgcc atgctgcgcg
    16801 cgccaccgag aatggattca cccccggatg cttccagaac cagcgatctt cataattgat
    16861 cagcgcttca aggtaacgtg gagacacatc ttcgattgtt accggataac gccagatgcc
    16921 atcagcatcg gcgaagcgcc agagcggcgt accatcctgc gccacgacca ctcgtgcggg
    16981 attgacttca tgcagcggta gaggccagag tttatctgct ccccacgctg caagaaaaag
    17041 gagaaatggc acagctgcca gcgttatcca gcagccgcgt ttggttaaca agcgaagcat
    17101 ttacggtcgg acaatcagca gatcttcagc cgcgccggtc gcccgccatt ggggaacata
    17161 cattgattcc accatcggtt gcggtacctg atacgttccc ggcgtcaccg cccgcgccag
    17221 atacaccagc gttaccggtt ggtattcatc aacggcaacc gccgccacaa agcgatcgtc
    17281 acggaactca atgtgcttaa tgctcgcctg ctgcatctgg ttcagtaagt tttgcacttc
    17341 gtcaccactt tgctccaggc tggcgctacc gttcgccaga ttctgatttt ccagttccag
    17401 acccgcaggc agcagatcca cgactaacgc atccggcacg ctgttactgg cttttacctg
    17461 caaccagacc agcaccagat cgccgctacg taacgagtcc agcgatttgc tcttaccatc
    17521 agtaccaaga atatgacgct cgatttgcag cacattgttc gccggtaaag gtgcggattg
    17581 cggataaccg ctggcatcca tacgcaacca taacggctga tcgccactgt ttctcacctg
    17641 caaggtggca agttgatcgc tattcagatt gctgtttagc gttttctcgc ctgtcagcgg
    17701 ctcagctgag aaagaggttt gcgcctgcca tttacccggt aaatcctgaa tcgtacgggc
    17761 agccaggaac aacgcgttac tttcctgcgt cgatagccag cgttcaccaa acgcctgctg
    17821 cgaaagtgtg ttcagcaaag tgtactgctc atcgggtagc agtttatttt cttccagcaa
    17881 ggagagcatt aacgcgttgt cgcgcagtgg actaccgtaa tcacccagcc atatccgctc
    17941 atcactattt ctcggcgttt tcagcgccag cacaatcgct tcttcaccac gcgttgcatc
    18001 acccatggtt ttcagcgcaa tgccaagttg cagcagcggt aaaccagaag cggcatctgc
    18061 acgatgctcc cagatttcac gcagcgcacc cagtggtgct ttttgctgac gggccagcac
    18121 cagcgcagcg taagactgaa cggcgaattt actggctttg agattatccg cgtacgggat
    18181 cgacatcatg cccggatctt gtaaatagcg cagcagacgc tcattacccc ggttaatggc
    18241 gtctgtcggc acgctgtaac cctgttcgcc tgcgcggacc aggaaatcca tcacgtaagc
    18301 cgtcagccag tactcttcgt caccgttttt atcccacagc gcaaagccgc cgttatcacg
    18361 ttgcatttgc agcaaacggg aaatgccgat atcgaccgat gcacggcgtt tctcatcact
    18421 gtcgcctttg atgcccaacg cctgcagttg ggctgcgttg gtataaagtg acgggaacag
    18481 gccgctggcg gtttgctcaa gacagccgta cggatacgct tttaactctt tgatataacg
    18541 tgcaatgttc agcggtggtt tgccgctcaa caacaattgc ccttccagcg taacaggcga
    18601 gaagttttgc aatccatccg ccggaatcgc ccatgtctca ccaggctgta acgccgtacc
    18661 gtaattaacc gtttgtgccg ggaacgccgg acggacgccg attttccact gcttatgctg
    18721 atcggcaacg gtttcacccg gtaacgctaa cccgctaatg gtggcctgaa tttctccatc
    18781 gccataaccc ggcaatgctc gcaccgggat aaacagcgta gtacgcacac ctggcgctaa
    18841 ttcaacggct gcgggtgaat cgctgaccag ttcaagcaaa ccactggcgg tcagggcaac
    18901 gttcagtttt tgcggtttat cggtaagatt agtgatatcc agcgtcagac gcgaggtatc
    18961 gccactcgcc ataaagcgcg gcatgttcag ttcagcaatc accggtgcgg caactatcac
    19021 tttgctttcg ttgctaccga aatcatctgc cgtccaggct tgcgccatga cgcgcaattc
    19081 accgttaaaa tcgccaatcg gcagtgtaac cgagccttcg ccctgttcgt tgagcgttac
    19141 cggcagcgcc tgctgcgcga caatattgac gtgattgacc agcggtttac caccacgttt
    19201 cagctcatca ccatcgccac cgaaacgcag agctgccaga cgcccctgac cttcaataac
    19261 ctgaccgtaa atatcgtaaa tgtctgcgcc atagcgtttc tgaccaaaga acgcctgcca
    19321 cggatctggc gtgacgtagt cagtaatatt cagcacacca ctatcaacgg cagacaccag
    19381 cacattcacc tgtttaggct tctcgccatt tttagtgctg gctttaattt tcacggttaa
    19441 tggttgattg ggacgcattt ttgctggtgt ttccagcgcc agatcgaggc gacggttttc
    19501 atcgccaagc ggcagatgca acacaccaac cgcgcgtttt ggcgtcgcgg agcgagattt
    19561 atcgctagga cgtaccacca gcgtacttaa atacagatca tgacgattcc aggttttatc
    19621 gaccggaatc gtcagatcca gcccttgagc cggaacatca atctcttgcc accacagcgg
    19681 cccttcactg gactcgacca tcgcataacc tttacccgcc gttggcgcgg cgatatgcaa
    19741 cttaatggtg tcgccagggc gataactggc tttatccagt ttcagcgtga cacggtcggg
    19801 tcgcactgcg ccgctaccgt cgctgttgtc ctgccagcta tagccagccc agaaacgaac
    19861 actactgacc gcttcattcg gcgctttgac ttccagacga taagcgcccc actctaccgg
    19921 aaaactgacc ttgccggttt cgtccgcttt cagatccaga gtttgttcat tttcgatcag
    19981 atctttttga tcaaactgtg actgccagcc ttcatcttct gaccagttcc agtagtaatc
    20041 gcggcgttcg cgaatcaggc gcacctgcaa gcccgacacg gcttttttca cgccttgtgc
    20101 atcgctataa acgatgtcaa aagcggcgtt actgccttca tcaacaatgg gctgtttcac
    20161 cgtgctgtca gtgcgataat cgtaaaccga tttcaaggcg aactgcggac ggagccccgg
    20221 caatgcattg gcaggccaga tagcctgctc agcgcggcgc gtcaccgggc gaccgcccga
    20281 ttccagcaaa ctgccctgga aaataacctg caatggggaa tgcgtttcct tccactggct
    20341 ttctgtagaa acttcgccgc gccctttatc atccagcgtc aactgaactt catccagcgt
    20401 gcgggaaagg ttttcggcag ctatatcgcc gaattcaaaa ccaggtaagg ctgacacagc
    20461 ctcacgcagt gggcgcagga aaagttgccc ttgcaaagta ttaccattag caggcgcacc
    20521 gtacaggtag tatcccacca cggagaattt cacttcatca ttcggcgtaa gcggggtttt
    20581 ctcaccggtc agattcagcg ccatacgctc tggcataaaa tcttcgacgt ggaaatccca
    20641 catccggtac tggttatcgc ccgtgttggc gcgaatatgc cacataccgg ttgccgcatt
    20701 gctatcgagt ggccaggtaa agtggtagag gccattctcc ggctgactaa cgacgctcct
    20761 gagtacctgc ccgtcaggtt taatcacgtc taacttgatg ggttgatcgg gcaatgcttt
    20821 accgtctgca tcgcgcagta aaccattgag gattaccgtt tcacctgggc gataaagatc
    20881 gcgtgggcca aacatgaaaa actgtttgct atagcctggc gcgccagcaa tgttaaattc
    20941 tgctaagtcc agcgccggaa gttttaaatc gagtagcgtt gtctgaccgt ctttacgtgc
    21001 cagcagtaat gccgcatttt tatcattttc cagctgcaca tgcccctgag cgtcactggt
    21061 tgcctgagtc agagtctgcc ctttctcatt taataaagag acttcaattc cttgctgggc
    21121 cgcgccgttt tccagacttt gggtaaagat atccagacga ttgtgataac ggtgagctga
    21181 aacgccgata tcacttaacg taaacagcgt cgcgggatta ctgtaatcgt aacgtccagc
    21241 ctgattcatc acagccagat acacgcccgc ctgctgaagc ggtttgatat cgcccagcgg
    21301 cagcaataat ttttcacggg tgttacgcgc aggattgaga tcaaaccgtc cggtgtagac
    21361 cagatccgcc atctgcagca gtttgtctga ctgccagttc gccagcgaat tgcggtattc
    21421 ccattggcta atgaatgctg gcagagattc tggcttaacg cggaagaagt taacatcgac
    21481 attattaacg ttgagcgcca ttaccggcag cccttcaacg actttgccag gcagcagcga
    21541 accacggctg gcaaaaccga cgctgggttg gatgtcgcgg gtagttatag ttttttcgta
    21601 atctttactg aaggttgcgt tgttgagtgc tttgacctcc ttgccaatag taacgatcaa
    21661 atcacgtttc ggttcgaggt ggcgtaaacg tagctcttta agattatctg acagctccca
    21721 ggcaccatcc actttgccgc tttttttatc gacgacatga ataacgcgtg agaaatcctg
    21781 atccgggtcg agagggatgg agaacgtcag caccagcgtg gctgcaccat caagttggac
    21841 ttcagagaga tccagcaatg tcagcacctt accggcactc tgttgggcca gtttttgtct
    21901 ctccggcacg gagagttttg ctgaactcgc gttttctgaa gaggccgctt tagtaacttc
    21961 agaaggcgca tcttttttca ccgctgttgg cgcgttatcg ttgttgtcgc accctgccag
    22021 cgccagcatt agcatgcagg cggctacgcg taactttttc atttttcatc cctgccacaa
    22081 tggcccgtta gcaacgtcga ataattatta tgcgtgagaa tttacgttat gtaattcagt
    22141 atcacgccca tacaatccga attttagtga gaattgtggc tcagacagcc aaatcagcct
    22201 ctttcgcttg tcgcttttca ctaaacatcc gacaatttag cctaaccccg gcaaaaatgg
    22261 agatgcctat gtccacgaca tggtttgtag gagccgactg gctcgccgaa catattgatg
    22321 acccggaaat tcagattatc gatgcccgca tggcgtcgcc tggacaggaa gatcgtaacg
    22381 ttgctcagga gtatctgaat ggacatattc ccggcgcagt gttttttgat atcgaagcgc
    22441 tttctgatca cacttccccg cttccgcaca tgctgccgcg cccgaaaacg ttcgccgtgg
    22501 cgatgcgtga attaggcgtt aaccaggata agcacctgat tgtctatgac gaaggtaatc
    22561 ttttctcagc cccacgagca tggtggatgc tgcgcacctt tggtgtagag aaagtgtcga
    22621 ttctgggggg tggacttgca ggctggcagc gcgatgatct gctgttagaa gaaggtgcag
    22681 tagagctgcc ggaaggagag tttaacgccg cgtttaatcc tgaagccgtg gtgaaagtaa
    22741 ccgatgtatt attggcaagc catgaaaata cggcgcaaat tattgatgcc cgcccggctg
    22801 cacgttttaa cgcagaagtt gatgaacctc gcccaggttt acgtcgcgga catattcccg
    22861 gagcactgaa tgttccgtgg acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg
    22921 aactggatgc gatatttttt ggtcgcggcg tcagctacga caaaccaatt atcgtcagct
    22981 gcggctctgg tgtaacggca gccgtggttt tgttagcact cgcgacgctg gatgtgccaa
    23041 acgtgaaact gtacgacggc gcatggagtg aatggggcgc gcgggcagat ttaccggttg
    23101 agccagtgaa ataagtattt tacaggcaat aaaaaaccgc cgaatttggc ggttttttat
    23161 tgctggtctg gttcgcggcc tttccagcag gttgtattac cgtagtaatg caagcgcgtc
    23221 tcagcggaga caatactcgc cagtaactct ctttttgtca agcaaaagag agttattatt
    23281 gttctgttag tatattatcc actgcggccc tttccgccgt ctcgcaagcg ggcgctggct
    23341 taaggaaagg atgttccgcg accgttagag caagcgcgtc tcagcgcttg ctatcgcggc
    23401 aatatcgcca gtggtgctgt cgtgatgcgg tcttcgcatg gaccgcacaa tgaagatacg
    23461 gtgcttctgt atcgtactta ttgtttctgg tgcgctgttt tcccaagtaa ataataaccg
    23521 gagtctctcc ggcgacaatt tactggtggt taacaacctt cagagcagca agtaagcccg
    23581 aatgccgccc tttagggcgg catattttag attatccggt tttgtttaaa gtcacgcaaa
    23641 aaacctcccc agcgacgttc atagaatggc acaatatgtt cggtaataaa gtggctaatt
    23701 cctttttccc cttttttcac ctgacaaata tcgattggtt catcgccagg taatgtatcg
    23761 gtcgctacac ttcccgtcgc ctgaataatt tcttcgatat caccatcggc ttcaatgcca
    23821 ataaataaat taggctgtgc ctcttcgttc tctttaattg aacaaataaa agcacgcttc
    23881 accggcttaa tggttttaaa taaggtggtg agtgaatcaa tcatttgtgc tggcggctct
    23941 gcaacttccg ataatatcag cgattcaccg ccttccagga tttcctggct gctcagcgga
    24001 tttccctctt caccaatcaa caaactgatt tcacgcggca taaattcttt accggtcggc
    24061 agtttggcat taaggaagag cgtttcgcca agggtcatct caaacaatgt gcgaacgggc
    24121 attacgacaa atgcctgttc gtcttcaacc gcctgttgaa gcgcttctaa cgaggtgaaa
    24181 aaaggaatga cgctggtgcc gtcttctttt tcccagtgct gtaaatcaag cgcactatct
    24241 tcaaccacag cctcgccctg cgccgccgta ccaggcaccc agacggtgga ttccagtaga
    24301 gtacggaaaa aggccgggcg gtgtgccggt tcggttgctg ctttttccag caggtcttca
    24361 agttcgtttt ttgtttcgga cataagaacc acaattcatt caacgttcgg ggcgcaaatg
    24421 cctgatgcgc tacgcttatc aggcctacaa gggattcgca atttgttgaa tttgcagaat
    24481 ttgtaggccg gataaggcgt ttacgccgca tccggcatat tagtttacgc cgttaacaga
    24541 ttagctatcg tgcgcacacc aagtcccgta gcgcccgcag accactgttc aaccggcgct
    24601 ttacggtaag tcgccgagca gtcgatatgc agccagcctt gctgatagtt ctcaacaaag
    24661 tgcgacagga agcccgccgc cgtgctcgcg cctgccggat acgccgcgct tccggtattg
    24721 ttcagttcgg caaagttaga cggcagctgg ctgcggtgga attccgccag cggcagacgc
    24781 cagaatggtt cgttctcttg tgaggcactc gccagcaaac gaccggcaag cgcatcgtca
    24841 aaactgaaca gcgcgtgata atcattaccc agcgcagttt tcgccgcccc ggtgagggtc
    24901 gccgcatcaa tgatcatttc cggtttctgc gcactggcat caatcagacc atcggcaagc
    24961 accagacgcc cttcggcatc agtgttcatc acttcaactt ttttaccgtt gcgataggtg
    25021 atgatatcgc ccagcttgaa cgcattgccg ctaatcaggt tatccgcaca gcagaggaac
    25081 agcttcacgc gcttgttcag tccgcgcgta atggcaaatg ccagcgcccc ggtaaccgtt
    25141 gccgcgccgc ccatgtccga cttcatcgag tccataaacg cagtctgttt gatgctgtag
    25201 ccgccggagt caaaagtgat acctttacct accaggcacg cgtacactgg cgcttcttta
    25261 tcgccagttg ggttgtaatc cagcgccagc aataccggag aacgttctga agcgcgtccg
    25321 acggtgtgca gccccatata accttgctca cgcagatctt cgcctttggt gatccgataa
    25381 gtcacacgat cgcccgcgac gttgctgatc agatcaacag cacgctgtgc cagttgcgat
    25441 ggtcccaatt cttctgccgg tgcgttgatg gtgtcacgca cccagtcgat gatcatcagg
    25501 cggttatcca gttcctggcg ctgggcatcg tccagatccg gccactccac tttacgcgtg
    25561 cctttcgggg ctttgtaacc ttgccagaat gcccagcagc gatccgcatc ccagccttca
    25621 ccgcttaact gaacatgctt gatgcccaga ccgtcaatct tgcgcgccgc acgctggatc
    25681 agccccagat cgtctgcccc gttcaaatgc agggtaatgc cgtcattatt aatgctgtaa
    25741 gttgcttttt ctccccagcg cgcgtcggca ggttgggtag agagggtaat cttcatcgct
    25801 tctgtcattt tagttatcct tctttgtaaa agggccgcca gttggcagcc ccaggatttt
    25861 tccgctaaat gattcgcgtt gcaggaaggc ggcaagtgag tgaagcccca ggagcataga
    25921 taactatgtg actggggtga acgagcgcag ccaacgcatc tgcggcgtga agcatgacgc
    25981 ggaaattatt cggcctcgtc cagccagact aacaaaatcg cttcgaggat tttctcgttg
    26041 gatgcctgcg ggtcgtcgtc gaaatcttcc agatcgcaaa tccactgatg catatcggtg
    26101 aatcgaaccg ttttcggatc aagatcagga tacgcatcgt acagtgcttc gccaatttcg
    26161 cggctatcgg tccacttaag tcccatacta acctctgtta atgctcacgc gcatggttga
    26221 tagtgtaacg cgggatttcg actactaaat cttcgtcggt aacgcgtgcc tggcagctta
    26281 aacggctttc gggctccagt ccccaggctt tgtccagcat gtcgtcttcc tgctctgagc
    26341 tttctggcag cgagtcaaaa ccttcacgaa cgatgcagtg gcaggtggtg caagcacagg
    26401 atttttcaca ggcgtgttca atctcgatac cgttacgcag cgctgcgtcg agaatggttt
    26461 caccgctatt agcttccaga acagcgccat cagggcagag gtcctgatga ggcaaaataa
    26521 caatctttgg catattaaac ctcgtccacg gaatggcctt tcagcgcacg acgaaccgac
    26581 tggtccatgc ggcgagcggc gaaatcctgg gtttgtttgt ctacgttttt aatcgcttgt
    26641 tcgatggcgt caacatcatc gccctgagcc acttcactca ggtgagcggc agcattgtca
    26701 atgacctgac gctctgcggc gcttaacagc gcggcatcag cagccagcgc gccgtgcaga
    26761 ctttccagca cacgcgccgc ttctactttt tgttctgcca gcatacgggc ttttacgtcc
    26821 tgctcggcat agctcattga atctttgatc atcgaagcga tttcgctatc ggtcagaccg
    26881 taagaaggtt tgacctgaat agacgcctca acgccggtgg atttctccat cgccgtcacg
    26941 ctcaaaagac cgtcggcatc gacctggaac gtcacgcgaa tatgcgcacc gccagccggt
    27001 agcgccggaa taccacgcag cgcaaaacgc gccagtgagc ggcagtcctg caccagttcg
    27061 cgctcaccct gcattacatg gatagacatc gccgtctgac catctttaaa ggtggtgaaa
    27121 tcctgagcgc gggccaccgg aatagtggta ttacgcggaa tcactttctc caccaggccg
    27181 cccatcgttt cgaggcccag cgacagtggg atcacatcaa gcagcagcat ttcgctgtcc
    27241 ggcttgttac ccaccagaat atccgcctga atcgccgcgc caatagcgac gactttatcc
    27301 gggtcgatgg aagtcagcgg tggacgaccg aaaaattcgc ctacccgttc acgcaccagc
    27361 ggcacgcgag tagaaccgcc aaccatcacc acttccagca cttcatcagc ttctacgccc
    27421 gcgtctttca gcgcacgacg acaagccagt aaggttcgtt ttaccagtgg cgcgatcagt
    27481 tcattgaatt gttcacggct gatttcgccc tgccagcccg caacgttaac ggtcacggag
    27541 tccgcatcgc tcagcgcgat tttggctgca atggcggcat ccagcagttc acgctgaacg
    27601 cggttatcgc tacgatcagg aatgtccgcc tgctcgcgaa tgtaatccgc cagcagatgg
    27661 tcgaaatcat cgccgccgag cgcggaatca ccgccggttg ccagcacttc aaacacgccg
    27721 cgacttaagc gcagaatgga aatatcaaac gtcccgccac cgaggtcata aacggcgatc
    27781 acgccttcct gaccggaatc cagcccgtag gcgatagccg cagcggtcgg ttcgttaagt
    27841 aagcgcagaa catgcaaccc cgccagacgc gccgcgtctt tggtgccctg acgctgggca
    27901 tcgtcaaagt acgccggaac ggtgataacg acaccatcca gctcgcctgc cagggcttca
    27961 gttgcccgcg ccgccagtgc tttgaggatg tccgcagaaa cgcgcaccgg gttcagcagc
    28021 cccgccgccg tttcaatcat tggcaggccg ttttcgctgg cctggaattg ataaggcaaa
    28081 tgcggatagc gttgctggat atcagccagc gagcgtccca tcaggcgttt aacagaacta
    28141 atggtgttgg cggtatcgag cgctgcgtta gtacgcgcgt cataacccac cgaatgccct
    28201 tgctgttgat agtgaacaac agatggcagc aggtgacggc cttcatggtc ggctaacgtt
    28261 tcggcctgac cgctgcgcac tgtcgctacc agcgagttgg ttgtgcccag gtcaataccg
    28321 gccgccagac gacgctgatg cggcgcagca ctcaaaccag gttcactaat ttgtaataag
    28381 gccatgttta gcttccagaa attaaaaatc gagcagtttt tcttcgagtt gttcggcact
    28441 gcttcgcagt ttatcgagaa aacgcagctt acgcacggta tccgccgccg cgtcccacgt
    28501 ctcgttgtct aactgttcaa ccatcaactg atggcgggta tcaaacatct ttttcacacg
    28561 tttgataaag ctttccagcc gcgcttcatc tttcgcctgt tcgatctcgt ccagttcttc
    28621 gcgcagctcc agctgttcca tcaggaacgc ggtgtcgcgc acagtatgct gctcgctggc
    28681 gagatcaaaa ccgtgcaaag aaagcaaata ttccgcgcgc attaacggat gacgcagcgt
    28741 ttgccaggcc tggttaatgg ttgcagattg ctgtacggcg gcgagttgtt ccgcctggct
    28801 tccgctggcg aatttatcag gatgatactg acgttgtaga tcctgaaaac gcaggctcag
    28861 tgcctgggta tcgagttgat aacgggcagg caagccaaag agggtgaagt aatccataac
    28921 attctcaggg ctgcgatccg ccgcgataac ggcgaatcag gtaaaacaaa ccccacgcgc
    28981 aggcgaccac ggtggggtta tcggtatgcg catcaaacgt ggaagctttc gccgcaacca
    29041 cactcatctt taacgttcgg gttggtgaat ttaaaccctt cattcaggcc ttcttttacg
    29101 aagtccagct gcgtaccgtc cagaaattgc aggcttttgc catcgaccac gactttcacg
    29161 cctttgtctt caaacacgat gtcttccggc gtcggttcgt caacaaattc cagtacataa
    29221 gccatacctg aacatccgga ggttctcacg cccagacgca ggccaaaccc tttgccgcgg
    29281 ttagccagaa aggtatttac tcgcgctgct gcactgtcgc tcagtgtaat cgacataacc
    29341 aaacctcaac tcttattttg cttcacgttt gcttttatag tccgcaatgg cggctttgat
    29401 cgcgtcttct gccagaatag aacagtgaat tttcaccggc ggcagttcaa gttcttcagc
    29461 aatatcggtg tttttgatcg cctgcgcttc gacgagagac ttccctttca cccattcggt
    29521 gaccagggag ctggaagcga tagcagaacc gcagccgtaa gttttaaaac gcgcgtcttc
    29581 aatgatacct tcatcgttga ctttaatctg caacttcatc acgtcgccac aggccggtgc
    29641 acccaccatg ccgctgccga cgttctcgtc gttgttgtca aaggaaccca cgttacgcgg
    29701 attctcgtaa tggtcgataa ctttttcgct gtaagccatt ataaattctc ctgattccga
    29761 taccgattaa tgatgagccc attcgatgct gttcagatcc acgccctgct tgtacatttc
    29821 ccacagcgga gaaaggtcac gcagacgacc gatggattta cgaactaact cgatggtgta
    29881 gtcgatctct tcttcagtag taaaacgacc taaagagaaa cggatagagc tatgtgccag
    29941 ctcgtcgttc agccccagcg cgcgcagcac gtaggacggt tcgaggcttg ctgacgtaca
    30001 ggcggaacct gaagaaactg cgaggtcttt cagcgccata atcagcgact caccttcaac
    30061 gtagttgaag ctgacgttga gaatgttcgg cgcaccgtgt tccaggtcac cgttcaggta
    30121 aacttcttcg atatctttga tgccgttcca cagacggtta cgcaggccgc gcagacgttc
    30181 catctcggtc gccatctctt cttttgcgat gcgataggcc tcgcccattc cgacgatctg
    30241 gtgaacaggc agagtgccgg aacgcatacc gcgctcgtga ccgccgccgt gcatttgcgc
    30301 ttcgatgcgt acgcgcggtt tacgacgtac atacagcgca ccgatacctt tcgggccata
    30361 gattttgtga ccggagaaag acatcaggtc aactttcaac tggctcaggt cgataggcag
    30421 tttgcccacg ctctgggttg catcaacgtg atagataatg ccacgagcac ggcacatttc
    30481 gccgatagcc gcgatatcct gcaccacgcc gatttcgtta tttacgtgca tgatggaaac
    30541 aaggatggtg tcgtcacgca tcgctgcttc aagttctttc aggtcgataa tgccgttacg
    30601 ctgcggtgcc aggtaggtga cttcaaaacc ttcgcgctcc agctgacggc aggtatccag
    30661 taccgctttg tgttcggttt tgctagtgat gatgtgcttg ccttttttct gataaaagtt
    30721 ggctgcacct ttgatcgcca ggttgtcaga ttcggttgca ccagaggtaa agacgatttc
    30781 acgcggatca gcgccgacca gatcggcaat ctgattacgg gcgatatcta ccgcttcttc
    30841 agcctgccag ccgaaacggt gagaacgaga ggccgggtta ccaaaggttc cgtccatcgt
    30901 catgaactgc atcattttct cggcaacacg cgggtccacc ggcgtggttg cggagtagtc
    30961 gagataaatc ggtaatttca ttgctctata aactccgtac atcactcaat gcaaggaatc
    31021 aggctaccgg ctggatgtac gaccgtgttt acggagtatt tagcactccg gcctgattct
    31081 gaattctttt tattaagcgc gtaacttaac gtcgatcgcg tcttgtgtgc gggtgcgtgg
    31141 cgcgtcgtga gtatgctgac gaccagacac atccagcact tcctggttat taaccagttc
    31201 gcctaaagta atgttgttga gaaaaccggt gagacggtcg ctcaaatcac gccacagcgc
    31261 gtgggtcagg catttatcgc cgccctggca gccgccttta ccctgacaac gggtggcatc
    31321 tacagattcg tcaacggcgc taatcacttc gccaacggcg atgctgctgg catctttgcc
    31381 taacagataa ccaccgcctg gtccacgtac gctggaaacc agaccatttt tacgcagacg
    31441 ggaaaacagt tgttccagat aagaaaggga aatgccctga cgttcggaaa tatcagccaa
    31501 cggtaccggg cccgcttcag agttgagcgc aacgtcaagc attgcggtca cggcatagcg
    31561 ccctttagat gtcagtctca tgtcttactt cacctcaaac tcgcccctgc ccggggtttt
    31621 ttattgtaaa agtgggggta ttgaatagca gggtcaagtc tgacattccc gagtaaattg
    31681 gtcaactatt tacttgactg atttagtcgg gtatttaacc ttcagtgcca tttttttatc
    31741 gtggcgtttg tacctgttgt cggatgcggt gtgaacacct tatccgacct acggttctgt
    31801 acattgtagg cctgataaga cgcgttaagc gtcgcatcag gcaactactg ttattcggcc
    31861 ttgttacctt tattctgctg ctcaatagaa gccagaatcc cgcgcaggat attcaattcc
    31921 tggctttccg ggcgcgcacg ggtaaacaga cggcgcaatt tattcatcac ctgccccgga
    31981 tggttttcac ggataaaacc ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc
    32041 tccagatcat cgaccagcgg atacggcgtc tcttcatgct cgacctgctc gccgttttcc
    32101 tgagttgcca gccaggccat gcgcacttca taggcaataa cctgaaccgc catcgccagg
    32161 ttcagcgagc tgtattccgg gttagccgca atcgcgacat gataatggca tttctgcaac
    32221 tcttcattgg tcaagccgac gcgctcgcga ccaaacacca gcgccaccgg ggtatttgcc
    32281 gcttcagcga cgcttttcag gccgcattcg cgcgggtcga gcatcggcca tggcagcgtg
    32341 cgggaacgtg cgctggtgcc caccaccaag ctacaaccgg ctaacgcttc gtcgagcgta
    32401 tcgacgatgt gagcattacc aatcacatcg ctggcccctg ctgccagggc aatcgcctgg
    32461 gagtcgggtt tcaccagtgg attaaccagc cacaaattgg ttaatcccat tgttttcatg
    32521 gcacgggcaa cagaacccat attgccggtg tgtgacgtct ccaccagcac aattcgaata
    32581 ttttgcagca ttgtctttct tcatctaaag attattcacg catcttatca taaaacgaag
    32641 acagatgccg atctcgctgc tatactctgc gccgttttcc cgttctttaa catccagtga
    32701 gagagaccga tgcatccgat gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat
    32761 ttaattgcca aaaactatga aaccccggac gctgtagaag cgagccagaa aggcagtaac
    32821 gatttcgtga ccaacgtaga taaagctgcc gaagcggtga ttatcgacac gattcgtaaa
    32881 tcttacccac agcacaccat catcaccgaa gaaagcggtg aacttgaagg tactgatcag
    32941 gatgttcaat gggttatcga tccactggat ggtactacca actttatcaa acgtctgccg
    33001 cacttcgcgg tatctatcgc cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac
    33061 gatcctatgc gtaacgaact gttcaccgcc actcgcggtc agggcgcaca gctgaacggc
    33121 taccgtctgc gcggtagcac cgctcgcgat ctcgacggta ccattctggc gaccggcttc
    33181 ccgttcaaag caaaacagta cgccactacc tacatcaaca tcgtcggcaa actgttcaac
    33241 gaatgtgcag acttccgtcg taccggttct gcggcgctgg atctggctta cgtcgctgcg
    33301 ggtcgtgttg acggtttctt tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag
    33361 ctgctggttc gtgaagcggg cggcatcgtc agcgacttca ccggtggtca taactatatg
    33421 ctgaccggta acatcgttgc tggtaacccg cgtgttgtga aagccatgct ggcgaacatg
    33481 cgtgatgagt taagcgacgc tctgaagcgt taatgactca ggcgggtgat gtcactcacc
    33541 cgccctcgcc tttcaggcgc tattccgaaa tacttcctca ccgctttact ttctttcgtc
    33601 actctcccac catctttccc cgattaatgg atgataaaga agtcacaagg aaaggttatg
    33661 gcgctgccag tgaacaaacg cgttctcaaa attctgttta ttctctttgt tgttgccttc
    33721 tgcgtttatt tagtgccgcg cgttgccatc aacttcttct attatcctga cgataaaatt
    33781 tacggtcccg acccctggtc ggcggaatcc gtcgaattta cggctaagga cggtactcgt
    33841 ctgcaaggct ggtttattcc ttcttcgacg ggccctgctg acaatgccat cgcaaccatc
    33901 attcatgctc acggcaatgc cggaaatatg tccgcccact ggccgctggt cagttggtta
    33961 cccgagcgta atttcaacgt ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc
    34021 acgccgtccc aggccggatt gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat
    34081 cgcagtgatg taaacccaca acggctggtg ctgttcgggc agagtattgg cggggcgaat
    34141 attcttgctg ttattggtca ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc
    34201 acatttgcct cttatgcaac catcgccaat cagatgatcc ccggcagtgg ctacttactt
    34261 gatgagagtt acagcggcga aaattatatc gccagcgtca gcccgatccc gcttttactc
    34321 attcacggta aagctgatca cgttatccca tggcagcaca gcgaaaagtt gtatagcctg
    34381 gcaaaagaga caaaacggtt gatcctaatc ccggatggcg aacacattga tgctttttcc
    34441 gatcgtcacg gcgatgttta tcgcgaacag atggtggact ttatccttag cgcgttgaat
    34501 ccgcagaact aacccatgat cgctagcacg ataatcattc acaaaaccac cttaagacat
    34561 gctaatccac tggtcagaac agtttaagat gagaaaaatt ctgtgacgct tgccaacatt
    34621 tctgatgatt agcattccct tcgccattta cctgagcaaa ctttagctat tcttatcaat
    34681 tatgcttatg ggagatctac agatgatgcc tacgcttgct ccaccatctg tcctttcggc
    34741 tccccagcgc cgctgccaga tcttgctgac gctctttcag ccggggttga ccgccaccat
    34801 ggcaaccttc agcgagctta atggtgtgga tgatgatatt gccagtcttg atatcagcga
    34861 aacaggacgg gagatcctgc gctatcatca actcacactg acgactggtt atgacggtag
    34921 ctaccgggtt gaaggtacag tgcttaacca acgtttgtgt ttatttcact ggctacgacg
    34981 tggtttccgt ctgtgtccgt catttattac cagccaattc acccccgccc tgaagagtga
    35041 actgaagcgg cgcggaattg cgcgtaactt ttacgacgat accaatctac aagcgttagt
    35101 gaatctctgc tcccgacggc tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg
    35161 tctgtatctg caatattgtt tgctgcaaca ccacgctgga attacgcctc agtttaatcc
    35221 gctccaacgt cgctgggcgg aatcctgcct tgaatttcag gtagcgcagg aaattggacg
    35281 ccactggcag cgtcgggcgc tccagcctgt accgcctgat gagccactgt ttatggcatt
    35341 acttttttcc atgttgcggg ttcccgatcc attgcgtgat gcgcatcagc gagacagaca
    35401 attgcgtcag tctatcaaac gtctggtaaa ccattttcgt gagctgggaa atgttcgttt
    35461 ttatgatgaa caggggttat gcgatcagct ttatacccac ctcgcccagg cgttaaatcg
    35521 cagtttgttt gccatcggta ttgataatac cctgccagaa gagttcgcca gactgtaccc
    35581 acgcctggtg cgcaccaccc gcgcggcgct ggccggattt gaaagtgaat acggcgtcca
    35641 tctttctgat gaggaaagtg gtctggtcgc ggtgattttc ggtgcctggc taatgcagga
    35701 aaacgatctg catgaaaaac agattattct tcttaccggg aatgatagcg agcgagaagc
    35761 gcagattgag cagcagttac gcgaactaac gttactgccg ctcaacatta aacatatgtc
    35821 ggtaaaggca tttttgcaga caggcgctcc gcgcggcgcg gcactgatta ttgcgcctta
    35881 taccatgccg ttaccgctct tttcaccacc gctgatctat acggacctga cactgacaac
    35941 acatcaacag gagcagatcc gcaaaatgct cgaatcagca tgagggaaca actttcgggc
    36001 gcaaaaacat tgccggaagc gccaccagcg ccattaccca gaacacgccg tggcccagat
    36061 attgatacag gaaaccggcg aaaacggtca tgatagctat actgccgccc atcgcgacgg
    36121 cagagtaaac cgcctgtaaa cggatgactt cgctaccctg gcgagcggca atatagcgca
    36181 tggcggccag gtggcagacc gtgaaggtgc cgcaatgcag aatttgcacc actatcaacc
    36241 acggcaacgc cgtagttgct cccataatgc cccagcgcac tacgccgcaa atcgccgaga
    36301 tcaacagcat atcgcgtgca ctacaacggc ggaaaagttt attactcagc gcaaagataa
    36361 tgacttccgc caccacgccc agcgaccaca aataccccac cgccgaggcc gagtagccag
    36421 ctgcctgcca gtaaatggcg ctaaaaccgt aataggccgc atgtgccccc tgcaataaac
    36481 aaacgcaggc cagaaagcgc cagttctgaa gaaccagcgc caaccacgct gaccaaccgg
    36541 tgctctcctg ctggcggctt gccccttgtg gctgaatcgt cggacggatg agaaagccga
    36601 gcagcatgga tgccacgccc aatgttaaca gcgcgaggat cacctgataa tcaaacatag
    36661 tgaccagttt gcccgtcagc gccgagccaa tgacaaacgc caccgagccc cacagtcgca
    36721 ctttgccgta atcaagcggg aactgctttt gccacgtatt cgccagtgca tcggtcaacg
    36781 gtaccagcgg tgagaaaaag aggttaaagc caatcatcac cagcatcagc cacgctacgt
    36841 gcgcccccgc ccagaaggcg acagcaaaga gaagtgtcat cattgccagc acgcgcaagg
    36901 cggaaatcag gcgagaagga tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac
    36961 gggcaaccag acctgccccc aataacaggc cgatggtttc tggcgttaaa ccaatccctt
    37021 taagccagac gctccagaaa ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc
    37081 cgagcgccaa ccagcgcgtg gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag
    37141 tctggggtta atcgaatgtt ggtgcaagtt gaaaaataag aatattcgag cctgcttccc
    37201 gatgtctgta acgccagttc acaagcacta aaaaactgcg atccgcgacg catgttgcaa
    37261 aaataatgcg taattatgcc gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat
    37321 cgcgtaatgc cggcttcatc tcccctttct tccacgccat cagtaaagca atggaaggca
    37381 catttccggc aatgggacga aaaacaacct gtccggtatt aaaattattc atataaccgg
    37441 gtatcaaagt gacgcccagc cccatgccca ccagattcat ggtcaccaga atattcgttg
    37501 ccacctggac gatatttggc tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg
    37561 caagcgaacc ggaatacgcc ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg
    37621 cggtgatctc tttttcatgt gctaaagggt gatcaaccgg taacacaacc actaatggtt
    37681 cgtcaaaaag ctccagataa tcaatctccg ggctataaac aggatggcgc attaagccga
    37741 cgtcgagttc accacggcga attttttcct cctgttgcgt ggtgattaaa ctcaccagct
    37801 caatcaaggt gtctggctgt ctgagacgaa acatcggtaa tacttttggc agtaaattca
    37861 cttccgccga tggcacaaag ccaatggtta attgtctgtc ttcctgaaca gttttccgcg
    37921 cccgtaattt ggcattttcc gcttgttcga ggattgccag cgcgtcctgg agaaaacatt
    37981 ctcccgccgc cgtcaacgcg actttgcgct tatccctcac cagtaacgga acaccgacac
    38041 agttttcaag atcgcggatc tggctgctta acgaaggctg tgaggtatgc agtttttccg
    38101 ccgcacgggt aaagttcagt gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt
    38161 ccatcacctt ccccttgtta tcgaaaaaac gtctcagctg gtagaaatta actatttcac
    38221 atattagcaa ccaaccagca acatccttat ggcacaaaaa tagaaggtca atacatctta
    38281 tctttcagga ttaaaaaata tgaccacacc ctcagatttg aacatttacc aactgattga
    38341 cacccaaaat ggtcgggtca ctccgcgtat ttataccgac ccggatattt accaactgga
    38401 gcttgagcgt attttcggtc gttgctggct atttctcgcc cacgaaagcc agatcccaaa
    38461 acccggtgat ttctttaaca cctacatggg agaagatgcg gttgtcgtag tgcgtcagaa
    38521 agacggcagc atcaaggcgt ttctcaacca atgccgccac cgggccatgc gtgtgagtta
    38581 tgcagattgc ggcaacactc gcgcctttac ctgcccgtat cacggctggt cttatggcat
    38641 taacggcgag ttgatcgatg taccgctgga acctcgcgcc tacccacaag ggttgtgtaa
    38701 atcccactgg gggctaaacg aagttccttg tgtggagagt tataaagggc tgatttttgg
    38761 caactgggat accagcgcac cgggcctgcg tgattacctt ggtgacattg cctggtatct
    38821 ggatggcatg ctggatcgtc gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg
    38881 ggtgatcaac tgtaactgga aatccccggc agagcagttc gccagtgacc aatatcatgc
    38941 tctgttcagc catgcttctg ccgttcaggt attaggggcg aaagatgatg gcagcgataa
    39001 gcgtctcggt gatggacaaa ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca
    39061 atttggtcag gacggtcacg gtagcggttt cttctttact gaaaaaccgg atgctaatgt
    39121 ctgggtcgat ggcgcggttt ccagctatta ccgcgaaacc tatgccgaag cagaacaacg
    39181 tttaggtgaa gttcgcgccc tgcgcctggc gggtcataac aatatattcc ccacgctttc
    39241 atggctaaac ggcactgcca cactccgcgt ctggcatccg cgcggccctg atcaagttga
    39301 agtgtgggcg ttctgtatta ctgacaaagc cgcctccgat gaagttaaag ccgcttttga
    39361 aaacagcgcc actcgtgctt ttggtcctgc tggttttctc gagcaggatg actcggagaa
    39421 ctggtgtgaa atccagaaat tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct
    39481 ggaaatgggg cttggtcagg aaaagcgccg cgacgacggc attcctggca ttactaacta
    39541 tatcttttca gaaactgccg ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag
    39601 cgaaagctgg caggaagtgc tcgataaaac cgccgcttac cagcaggagg tgatgaaatg
    39661 agtgcgcaag tttcactaga gttacatctc cgcattagcc agtttctctt tcacgaagcc
    39721 agcttactgg acgactggaa atttcgtgac tggctggcgc agctcgacga agagattcgt
    39781 tacaccatgc gcaccacagt taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca
    39841 ccgacaacct ggatttttaa tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg
    39901 gaaacgggca tggcctgggc agaagagccg ccgtcacgca cccgtcactt aatcagcaac
    39961 tgccagataa gcgaaaccga catcccaaac gtatttgctg tgcgggtaaa ttatctgctt
    40021 tatcaggcac aaaaagagcg cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt
    40081 cgccgtctgg aagatgacaa ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg
    40141 gtaatcactt cccataacct gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg
    40201 ttgcggatgt gccggagggt gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt
    40261 tcaacgttgg cggcgagttt tatgccatta acgatcgttg cagccatggt aatgcgtcga
    40321 tgtcagaagg gtatctggaa gatgacgcca cggtggagtg cccgctacac gccgccagtt
    40381 tttgcctgaa aacggggaaa gcgttatgcc tgcccgccac cgatccgctc accacttatc
    40441 cagtacacgt tgaaggtagt gacattttca tcgacttacc ggaggcgcag ccatgagcga
    40501 tctgcataac gagtccattt ttattaccgg cggcggatcg ggattagggc tggcgctggt
    40561 cgagcgattt atcgaagaag gcgcgcaggt tgccacgctg gaactgtcgg cggcaaaagt
    40621 cgccagtctg cgtcagcgat ttggcgaaca tattctggcg gtggaaggta acgtgacctg
    40681 ttatgccgat tatcaacgcg cggtcgatca gatcctgact cgttccggca agctggattg
    40741 ttttatcggc aatgcaggca tctgggatca caatgcctca ctggttaata ctcccgcaga
    40801 gacgctcgaa accggcttcc acgagctgtt taacgtcaac gtactcggtt acctgctggg
    40861 cgcaaaagcc tgcgctccgg cgttaatcgc cagtgaaggc agcatgattt tcacactgtc
    40921 aaatgccgcc tggtatcctg gcggcggtgg cccgctgtac accgccagta aacatgccgc
    40981 aaccggactt attcgccaac tggcttatga actggcaccg aaagtgcggg tgaatggcgt
    41041 cggcccgtgt ggtatggcca gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac
    41101 ctcgataatg cagtctctga cgccggagaa aattgccgcc attttaccgc tgcaattttt
    41161 cccgcaaccg gcggatttta cggggccgta tgtgatgttg gcatcgcggc gcaataatcg
    41221 cgcattaagc ggtgtgatga tcaacgctga tgcgggttta gcgattcgcg gcattcgcca
    41281 cgtagcggct gggctggatc tttaaggaag cacgatgaaa gaaaaaacga tcattattgt
    41341 cggtggcgtg caagcggcgg caatggctgc ggcctcgcta cgccagcaag ggttcaccgg
    41401 tgagctgcat ctgttttccg atgagcgaca tcttccttat gaacgacctc cactctcgaa
    41461 atccatgttg ctggaagatt ccccgcagtt acagcaggtg ttacccgcta actggtggca
    41521 ggaaaacaat gttcatctgc attccggtgt aaccatcaaa acgctgggtc gcgacacacg
    41581 agagttagtg ttaaccaacg gcgaaagctg gcactgggat cagcttttta tagcaaccgg
    41641 cgcggcagct cgaccgctgc cgttgcttga tgcactggga gaacgctgct ttaccctacg
    41701 ccatgccggt gatgccgcca gactgcgaga agttctgcag cccgaacggt cagtcgtgat
    41761 tgtcggtgcc ggaactattg gtctggaact ggctgccagc gccacgcagc gcagcgcagc
    41821 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    41881 accaccgccc gtgcaacgct atcttttaca gcgccaccag caggctggtg tgcgcattct
    41941 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    42001 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    42061 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    42121 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    42181 tgcactacac cgctgtgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    42241 cgcaatgtta gggctaccgc taccgctact gccgtggttc tggagcgatc agtacagtga
    42301 taacttacag tttattggcg atatgcgtgg cgatga
//
LOCUS       DADLTD010000008        40981 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLTD010000008 DADLTD010000000
VERSION     DADLTD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.008"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..332)
                     /locus_tag="JN701_000325"
     CDS             complement(<1..332)
                     /locus_tag="JN701_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7362872.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCG"
     gene            complement(413..1840)
                     /locus_tag="JN701_000326"
     CDS             complement(413..1840)
                     /locus_tag="JN701_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY7362873.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTLADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1837..2544)
                     /locus_tag="JN701_000327"
     CDS             complement(1837..2544)
                     /locus_tag="JN701_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7362874.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2707..3684
                     /locus_tag="JN701_000328"
     CDS             2707..3684
                     /locus_tag="JN701_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY7362875.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3754..4236)
                     /locus_tag="JN701_000329"
     CDS             complement(3754..4236)
                     /locus_tag="JN701_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY7362876.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4471..7053
                     /gene="leuS"
                     /locus_tag="JN701_000330"
     CDS             4471..7053
                     /gene="leuS"
                     /locus_tag="JN701_000330"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY7362877.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7068..7649
                     /gene="lptE"
                     /locus_tag="JN701_000331"
     CDS             7068..7649
                     /gene="lptE"
                     /locus_tag="JN701_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY7362878.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7649..8680
                     /gene="holA"
                     /locus_tag="JN701_000332"
     CDS             7649..8680
                     /gene="holA"
                     /locus_tag="JN701_000332"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY7362879.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8682..9323
                     /gene="nadD"
                     /locus_tag="JN701_000333"
     CDS             8682..9323
                     /gene="nadD"
                     /locus_tag="JN701_000333"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY7362880.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9347..9957
                     /locus_tag="JN701_000334"
                     /pseudo
     CDS             9347..9957
                     /locus_tag="JN701_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            10217..10534
                     /gene="rsfS"
                     /locus_tag="JN701_000335"
                     /gene_synonym="rsfA"
     CDS             10217..10534
                     /gene="rsfS"
                     /locus_tag="JN701_000335"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY7362881.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10538..11005
                     /gene="rlmH"
                     /locus_tag="JN701_000336"
     CDS             10538..11005
                     /gene="rlmH"
                     /locus_tag="JN701_000336"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY7362882.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11036..12937
                     /gene="mrdA"
                     /locus_tag="JN701_000337"
     CDS             11036..12937
                     /gene="mrdA"
                     /locus_tag="JN701_000337"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY7362883.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12940..14052
                     /gene="mrdB"
                     /locus_tag="JN701_000338"
                     /gene_synonym="rodA"
     CDS             12940..14052
                     /gene="mrdB"
                     /locus_tag="JN701_000338"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY7362884.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14063..15151
                     /gene="rlpA"
                     /locus_tag="JN701_000339"
     CDS             14063..15151
                     /gene="rlpA"
                     /locus_tag="JN701_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY7362885.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15291..16502
                     /gene="dacA"
                     /locus_tag="JN701_000340"
     CDS             15291..16502
                     /gene="dacA"
                     /locus_tag="JN701_000340"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY7362886.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16612..16875
                     /locus_tag="JN701_000341"
     CDS             16612..16875
                     /locus_tag="JN701_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY7362887.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16976..17617
                     /gene="lipB"
                     /locus_tag="JN701_000342"
     CDS             16976..17617
                     /gene="lipB"
                     /locus_tag="JN701_000342"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY7362888.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17876..18829
                     /locus_tag="JN701_000343"
     CDS             17876..18829
                     /locus_tag="JN701_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY7362889.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19038..20003
                     /gene="lipA"
                     /locus_tag="JN701_000344"
     CDS             19038..20003
                     /gene="lipA"
                     /locus_tag="JN701_000344"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY7362890.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20104..20307)
                     /gene="tatE"
                     /locus_tag="JN701_000345"
     CDS             complement(20104..20307)
                     /gene="tatE"
                     /locus_tag="JN701_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY7362891.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20436..21224)
                     /locus_tag="JN701_000346"
     CDS             complement(20436..21224)
                     /locus_tag="JN701_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY7362892.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21317..21700
                     /gene="crcB"
                     /locus_tag="JN701_000347"
     CDS             21317..21700
                     /gene="crcB"
                     /locus_tag="JN701_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY7362893.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21755..21964)
                     /gene="cspE"
                     /locus_tag="JN701_000348"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21755..21964)
                     /gene="cspE"
                     /locus_tag="JN701_000348"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7362894.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22139..22699)
                     /locus_tag="JN701_000349"
     CDS             complement(22139..22699)
                     /locus_tag="JN701_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY7362895.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23288..24673
                     /gene="dcuC"
                     /locus_tag="JN701_000350"
     CDS             23288..24673
                     /gene="dcuC"
                     /locus_tag="JN701_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7362896.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24714..25394)
                     /gene="dpiA"
                     /locus_tag="JN701_000351"
                     /gene_synonym="citB"
     CDS             complement(24714..25394)
                     /gene="dpiA"
                     /locus_tag="JN701_000351"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HAY7362897.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25363..27022)
                     /gene="dpiB"
                     /locus_tag="JN701_000352"
                     /pseudo
     CDS             complement(25363..27022)
                     /gene="dpiB"
                     /locus_tag="JN701_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27371..28429
                     /gene="citC"
                     /locus_tag="JN701_000353"
     CDS             27371..28429
                     /gene="citC"
                     /locus_tag="JN701_000353"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY7362898.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28444..28740
                     /gene="citD"
                     /locus_tag="JN701_000354"
     CDS             28444..28740
                     /gene="citD"
                     /locus_tag="JN701_000354"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY7362899.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28737..29645
                     /gene="citE"
                     /locus_tag="JN701_000355"
     CDS             28737..29645
                     /gene="citE"
                     /locus_tag="JN701_000355"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY7362900.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAERSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29656..31188
                     /gene="citF"
                     /locus_tag="JN701_000356"
     CDS             29656..31188
                     /gene="citF"
                     /locus_tag="JN701_000356"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY7362901.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31192..31742
                     /gene="citX"
                     /locus_tag="JN701_000357"
                     /pseudo
     CDS             31192..31742
                     /gene="citX"
                     /locus_tag="JN701_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31717..32565
                     /gene="citG"
                     /locus_tag="JN701_000358"
     CDS             31717..32565
                     /gene="citG"
                     /locus_tag="JN701_000358"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY7362902.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACKGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLALTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGACAGYSARRKHYCKKGAFEPPPISIISGSSTGSVSNEISVQ
                     AAVPTC"
     gene            32645..34108
                     /gene="citT"
                     /locus_tag="JN701_000359"
     CDS             32645..34108
                     /gene="citT"
                     /locus_tag="JN701_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY7362903.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34219..35025
                     /gene="rna"
                     /locus_tag="JN701_000360"
     CDS             34219..35025
                     /gene="rna"
                     /locus_tag="JN701_000360"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY7362904.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPSNCGKTFVIDKVGY"
     gene            35255..35665
                     /gene="rnk"
                     /locus_tag="JN701_000361"
     CDS             35255..35665
                     /gene="rnk"
                     /locus_tag="JN701_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY7362905.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(35896..37134)
                     /locus_tag="JN701_000362"
     CDS             complement(35896..37134)
                     /locus_tag="JN701_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAY7362906.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            37355..37783
                     /gene="uspG"
                     /locus_tag="JN701_000363"
     CDS             37355..37783
                     /gene="uspG"
                     /locus_tag="JN701_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY7362907.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(37904..39469)
                     /gene="ahpF"
                     /locus_tag="JN701_000364"
     CDS             complement(37904..39469)
                     /gene="ahpF"
                     /locus_tag="JN701_000364"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY7362908.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(39640..40203)
                     /gene="ahpC"
                     /locus_tag="JN701_000365"
     CDS             complement(39640..40203)
                     /gene="ahpC"
                     /locus_tag="JN701_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY7362909.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            40515..>40981
                     /gene="dsbG"
                     /locus_tag="JN701_000366"
     CDS             40515..>40981
                     /gene="dsbG"
                     /locus_tag="JN701_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706462.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY7362910.1"
                     /translation="MTVIGYAFYSTFALTEKDKLMLKKILLLALLPAIAFAEELPAPV
                     KAIEKQGITIIKTFDAPGGMKGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSN
                     TLIEKEIYAPAGREMWQRMEQSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQA"
BASE COUNT        10180 a        10480 c        10071 g        10250 t
ORIGIN      
        1 tccccacagc acatagcggt ttgggcaacg aattgggtat aaatcaaccc tctctccgag
       61 ccaccagcaa ttaatgcttt ccactcttca ggggaagagc gatgccatgt cagatataag
      121 ccagctaaat cattggcatg accatcatca gtcatctgat aaatagtctg aattaaatca
      181 tcacgattat ttattcccca ggatgatgag agatcgaatt cttttatacc ggtatacatt
      241 tttggcgagt catgcgcggg tttgcaaccc tgattaagta tttccatggg cgcagaaagc
      301 gcaaaaagaa gatattgttc ttccttattc atttgttaat aatcccttaa gtgcattaac
      361 tgactacttt tttatattac ttgccgggct ttccgcacat taacgtcgac tgtcacccga
      421 acagatggag tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga
      481 tgacgcccag acagcccggc ccaccatcgt caacgggcat tggctgcggt tgcggctccg
      541 gttcaacagt ttgcttctcg gtaaaattcg ccgcctgaat taataatcga cgaataatta
      601 atgcttccag tggacaacga tcttctgcat caagacggct tacgatcggc attaatgatt
      661 cttctgtcgg ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataaga
      721 gatgttctgt atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct
      781 cgaccagtga acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa
      841 aacgcgtacg ggcgctgtcg ccggggacat aaagtagctg tttaccctga tcgttaacca
      901 gttcacgagc ttcaatcatc atcaccacta acgggtaata atcgacccga tgctgacggc
      961 accattgcaa tattaaagca tccgccaggg tggagggata ctcgcgccag tagtcacaaa
     1021 gttctgccag caagctttcg ccttctccac aatagacgcg ttgcgcgtat tcataataac
     1081 gcgcatcttc attatcaggt tcagcttccc gccacgaccg ggcgtaatcc agaagttcgg
     1141 ggatatccag gcgacctgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat
     1201 cggggatgat cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg
     1261 accaaatccg agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca
     1321 gatgcagaat ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt
     1381 ccagttcttc ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg
     1441 ctaataacac cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg
     1501 accagcgtaa ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct
     1561 gccaggcgga gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca
     1621 gaatttcatg ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact
     1681 gcgcaatccg tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggttcgg
     1741 tttctggatg aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag
     1801 tcgtttcctc tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag
     1861 cgtttccggg catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc
     1921 atcatcactg gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg
     1981 cgctaagaat tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg
     2041 accaaaggta taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag
     2101 atgaatacgc gactggatcc atagactttc ttcttcactc aaccagttat taagtacgcc
     2161 cattcgactt aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca
     2221 gcattcggct gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc
     2281 aagatagtca cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa
     2341 ccgagcggca tgaccgttct cgatcattcg gtgaattgtg cgcagcaggt catcacggtc
     2401 atcaattccc catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga
     2461 tgaatgcgaa ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa
     2521 tattttttgc gaccccatgt ccatataatt tatccttaaa cactcattta atgaatgtaa
     2581 actacatttc gagactcagt tatttatact tttctgacgc gacataatac gcataaaata
     2641 aggcttacgc aaaaatggaa cacataaaat ataatatgtt ttatctacaa ccataaggaa
     2701 atgataatga tttttacgtc aagttgctgc gataatttat caatagatga gattatcgaa
     2761 cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat
     2821 agcgcaattg actctccgga cgacgaaaag gccttttact ggctgaagct ggccgccgag
     2881 caaggtcatt gtgaagcaca atattcctta ggacaaaagt atactgagga taaaagccgt
     2941 cataaagata atgagcatgc catcttttgg ctgaaaaaaa ctgccctaca aggccatact
     3001 ttcgcttcca acgcccttgg ctggatactg gatcgtggag aagaccccaa ttataaagaa
     3061 gcggttgtct ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt
     3121 gggtggatgt acagaaatgg caacggagtc gcaaaagact atgcgctggc atttttctgg
     3181 tacaaacaag ctgcattaca aggccatagt gacgcgcaaa acaatctggc cgatctttat
     3241 gaagacggaa aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc
     3301 gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa
     3361 ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa
     3421 agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa
     3481 gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc
     3541 tggtataacc tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag
     3601 gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt
     3661 cgtgagactg aagatttact gtagagataa aaacaagggc cggggaattt cattcggccc
     3721 atattctctt tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa
     3781 ctggtcatga ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg
     3841 gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac
     3901 cccatgatga ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat
     3961 atctgccagc tcctgccata agctttcttt aatcacccgc ataaagacgc tgtcagattc
     4021 ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc ttacagctcg
     4081 cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc
     4141 ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc
     4201 gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac
     4261 tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta
     4321 aattaatgta tgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg
     4381 acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta
     4441 gtcgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata
     4501 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
     4561 agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg
     4621 ggccacgtac gtaactacac catcggtgac gtgatcgccc gctaccagcg tatgctgggc
     4681 aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
     4741 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
     4801 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
     4861 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
     4921 aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa
     4981 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag
     5041 tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat
     5101 cactggccag acaccgttaa aagcatgcag cgtaactgga tcggtcgttc cgaaggcgtg
     5161 gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
     5221 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
     5281 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
     5341 gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagctgtt
     5401 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
     5461 ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct
     5521 aaatacggcc tgaacatcaa accagttatc ctggctgctg acggctctga gccagatctt
     5581 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgaatt caacggtctt
     5641 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
     5701 cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg
     5761 ccgattccga tggtgacgct ggaagacggt accgtaatgc cgaccccgga cgaccagctg
     5821 ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat
     5881 ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc
     5941 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa
     6001 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt
     6061 atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat
     6121 gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg
     6181 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
     6241 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
     6301 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgaccca
     6361 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
     6421 ccggctgata tgactctaga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg
     6481 aaacgtgtct ggaaactggt ttacgagcac acagcaaaag gtgatgttgc ggcactgaac
     6541 gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aacgatcgct
     6601 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
     6661 gagctgatga acaaactggc gaaagcacca accgatggcg agcaggatcg cgctctgatg
     6721 caggaagcgc tgctggccgt tgtccgtatg cttaacccgt tcaccccgca catctgcttc
     6781 acgctgtggc aggaactgaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
     6841 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
     6901 cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc
     6961 caggaacatc tggtagcaaa atatcttgat ggcgttaccg tgcgtaaagt gatttacgta
     7021 ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg
     7081 caacattgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg
     7141 ataccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg
     7201 ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata
     7261 aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag
     7321 ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga
     7381 atgcgaccgt gttgatcccc ggccgtgata tctacccgat tagcgccaaa gtcttccgtt
     7441 cgttctttga taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa
     7501 aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg
     7561 cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg
     7621 cacgcgtctc caccacgctg ggtaactgat gattcggttg tacccggaac aactccgcgc
     7681 gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt
     7741 gcaggaaagc caggacgctg ttcgtcaggt agctgcggca caaggattcg aagaacacca
     7801 cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat
     7861 gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc
     7921 ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc tgctgttgat
     7981 cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgcgcttgc
     8041 gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt
     8101 tgctgcgcgc gcaaaacagc tcaacttaga actggatgac gcggcaaatc aggtgctctg
     8161 ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct
     8221 ctggccagac ggcaaattga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca
     8281 tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcacattgca
     8341 tattcttcag caactgcgtc tggaaggcag cgaaccggtt attttgttgc gcacattaca
     8401 acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc
     8461 gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa
     8521 tcgcttaagt cagacgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac
     8581 cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt
     8641 gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc
     8701 tgtttggcgg cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg
     8761 cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc
     8821 cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca
     8881 agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc
     8941 aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg
     9001 gtcaggattc actgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg
     9061 cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc
     9121 agcaatggct ggaagatcat ttgacacata accaggaaga tcttcacctt cagcctgccg
     9181 gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac
     9241 gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtactg acttacatta
     9301 accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt
     9361 aattcgtcat ggtgaaacgc aagcgaatat cgatggtctt tacagcggtc atgcgccccc
     9421 cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct acatggtgtt
     9481 tcctttgatc tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt
     9541 ctcagtgacc gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc
     9601 gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg
     9661 tggtgcaatg actggcagca tgcaatcccc acgaacggtg aaggatttca ggcattttcg
     9721 caacgtgtgg aacgctttat cgcaaggctt agtgaatttc agcactatca gaatatttta
     9781 gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc gtttaattgg catgcctgcc
     9841 gaggctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa
     9901 aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgataa tgcatgactt
     9961 ttctgttttt ttacgggtaa gccgcaacga ccattgacag acccgggcag gctgatattc
    10021 tccgcagcca gacttttttc gccagacacg actttgtaga aattgtttta caaaaatggc
    10081 gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca
    10141 attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt
    10201 cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga
    10261 tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga
    10321 ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt
    10381 tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc
    10441 cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg
    10501 tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt
    10561 gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc
    10621 gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga
    10681 catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg
    10741 cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct
    10801 ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt
    10861 gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc
    10921 ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac
    10981 caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa
    11041 actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct
    11101 ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct
    11161 gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt
    11221 gcctatcgcg cccagccgcg gcattattta cgaccgtaat ggtatccctc tggccctcaa
    11281 ccgcactatc taccagatag aaatgatgcc agagaaagtc gataacgtgc agcaaacgct
    11341 ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga
    11401 gcgcgcacgt tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca
    11461 agtagctcgc tttgccgtca atcagtaccg ttttccgggg gttgaagtta aaggctataa
    11521 acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat
    11581 caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac
    11641 gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac
    11701 cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt
    11761 accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat
    11821 tgaaacgctg ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg
    11881 ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ttgtttgttg acggtatctc
    11941 cagcaaagat tattccgcct tgttgaacga cccgaataca ccgctggtga accgcgccac
    12001 acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag
    12061 cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc aactgccagg
    12121 ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag
    12181 atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga
    12241 tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc
    12301 ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc
    12361 gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc
    12421 aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca
    12481 tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc
    12541 gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt
    12601 tgctaaccgc cctaacggta cgacgcataa atactttgcc agcgcaccgt acaaaattgc
    12661 ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca
    12721 caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa
    12781 tccgcaagtg gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac
    12841 gctgatgcgt cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc
    12901 ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa
    12961 aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg
    13021 ctggtttaca gcgccctagt tatctggagc gccagcggtc aggatattgg catgatggag
    13081 cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct
    13141 ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg
    13201 gcggtagatg ctttcggtgc catctctaaa ggtgctcaac gctggctgga cctcggtatt
    13261 gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt
    13321 atcaaccgcg acgtttgccc accgtcgttg aagaacacag gcatcgcgct ggtgctgata
    13381 tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg
    13441 ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca
    13501 gtagtgctgg tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag
    13561 cgccagcgcg taatgatgct cctagacccg gaatcagacc cactcggcgc gggctatcac
    13621 attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat
    13681 ggcactcagt cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta
    13741 ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg
    13801 atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct
    13861 ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt
    13921 attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt
    13981 gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg
    14041 aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg
    14101 aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc
    14161 tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact
    14221 gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga
    14281 tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa
    14341 cctgacggcc tctggcgaag ctttcgatcc gacacagctg acggcggccc atccaacgct
    14401 tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg
    14461 cattaatgat cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc
    14521 tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca
    14581 ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc
    14641 cctgcctgca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca
    14701 gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc
    14761 ggtaaccagt agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg
    14821 cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac
    14881 ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt
    14941 gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca
    15001 gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg
    15061 ccccttcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca
    15121 attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt
    15181 ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc
    15241 tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15301 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15361 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15421 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15481 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15541 gccaagcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15601 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15661 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15721 gtgtcgccat ggctgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15781 gctacgttaa cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg
    15841 ctgatggtca gtacagctcc gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg
    15901 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    15961 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16021 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16081 tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16141 tgctgacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16201 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16261 acatgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16321 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16381 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16441 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16501 aaaaattaaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaac
    16561 tcccgccttc tggcgggagt tgctatttaa ttacgttacg ccggagctga catgaaaacc
    16621 aaacttaacg aactgcttga attccctact ccttttactt acaaagttat ggggcaggcg
    16681 ttacctgagc tggttgatca ggtggttgaa gtggtacagc gccatgcgcc aggtgactac
    16741 accccaacgg taaaaccaag cagcaaaggc aactaccact cggtatctat cactatcaac
    16801 gccactcata tcgagcaggt tgaaatactg tatgaagaac tgggcaaaat cgatattgtc
    16861 cgcatggttc tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa ctcccctctc
    16921 ctcgctgtga tatacttttc ccccactttt actcattctc cacggagatg ccgttttgta
    16981 tcaggataaa attcttgtcc gccagctcgg tcttcagcct tacgagccaa tctcccaggc
    17041 tatgcatgaa ttcaccgata cccgcgatga tagtaccctt gatgaaatct ggctggtcga
    17101 gcactatccg gtattcaccc aaggtcaggc aggaaaagcg gagcacattt taatgccggg
    17161 tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg ggccgggaca
    17221 acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt
    17281 gaccttgctt gagcaaacag tggtgaatac cctggctgaa ctgggtatag aagcgcatcc
    17341 tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg
    17401 aattcgacgc ggttgttcat tccacggtct ggcattaaac gtcaatatgg atctttcacc
    17461 atttttacgt attaatcctt gtgggtatgc cggaatggaa atggctaaaa tatcacaatg
    17521 gaaacccgaa gcgacgacta ataatattgc tccacgttta ctggaaaata ttttagcgct
    17581 actaaacaat ccggacttcg aatatattac cgcttaattc catacatcaa tggcccaatt
    17641 tacactgggt cattactcat aatacctgct aatgctttac atctccttcg cgtttgtata
    17701 ttttctttat cacttgcaga aatgcggtgc cacgccttgc tccgtaactc acccctttga
    17761 ttaacctatt gaaatagttg attatggtga gcaaaagcgt tccgttccaa ttagacataa
    17821 gattattgta attttttaaa tattattttt ccatgaataa aattggagta agtgcgtgga
    17881 tagtaataat caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat
    17941 atttacaacc ttacgaaata ttgctcttaa ccttctgact atttttgaag ctgtatatgt
    18001 acataaaggg atcgttaatg cagcgaaagt gcttaatctg cccccctcgg caatcagtca
    18061 gtctattcag aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaagg
    18121 tgtcactcct accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc
    18181 cattcttggc gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc
    18241 gaccactccc tcggtcggag ccctggtcct tcctgtcatc tatcgggcga ttaaaactca
    18301 ctatccgcag cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca
    18361 gtttcaaacc gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca
    18421 tgttctgttc accgacaata tggtgttaat ttgccgcgag ggaaatccac tactctcttt
    18481 agaagatgac agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagagca
    18541 aaatttcagc ggtctgcggc agagagttca agaaatgttt ccggaccggc aaatcaattt
    18601 caccagctac aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat
    18661 tattcccagc cgtttttata acctgtttag ccgctgctgg ccgctggaaa aactgccttt
    18721 tccgtcctta aataaggagc aaatagattt ctctatccac tacaacaaat tcagcctgcg
    18781 tgatcccatc ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt
    18841 tacagccaga tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt
    18901 tcgcgaccgc tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc
    18961 aaaaatagtt gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca
    19021 actggaacac gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg
    19081 atgccgataa gatggccctt atcccggtta aaaacgtggc aatagagcgc gaagccctgc
    19141 tgcgcaagcc ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca
    19201 tcaaagccgc aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta
    19261 acctggcgga atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta
    19321 cccgccgttg tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg
    19381 aaccagtgaa actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct
    19441 ccgttgaccg tgatgacctg cgcgatggcg gtgcccagca ctttgcggat tgcattactg
    19501 ccattcggga aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgtggtc
    19561 gtatggatcg tgctctggat attctgactg caacgccacc agatgtgttc aaccataacc
    19621 tggaaaacgt accgcgtatt taccgtcagg tacggcctgg tgcagattac aactggtcgc
    19681 tgaagctgct ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctaa
    19741 tggtgggact gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc
    19801 atggtgtgac gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg
    19861 ttcaacgtta cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg
    19921 gctttaccca tgctgcatgc ggtccgtttg tccgttcttc ttaccacgcc gatttgcagg
    19981 cgaaagggat ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac
    20041 ccgctttttg aagcggtttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc
    20101 acgtcactct ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc
    20161 cgcagcatca tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc
    20221 gcccagcgta cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactactag
    20281 cagtttggta atactaatct cacccataga taccttcttg acatataacc ggcaacagaa
    20341 cgcgccttaa aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct
    20401 gtaacaaggt gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag
    20461 cgacggttgt ttaagacggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact
    20521 tccgccataa tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct
    20581 ataatccggc tttgcccgat atttttgttc ccgcactcgc ccgccgccac catataacag
    20641 gtggtatcca gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggcccg
    20701 cgaacccagg cggcaggaag taccaggatt tcagctccct gtaatgcctg tgccagcgcc
    20761 agctctggaa agcgtaagtc ataacaggtc atcagaccga ccttcatccc ttccacctcc
    20821 agtaacggag cgatttcatt accagcatca acacggcgtg attcctgaat ggcaaatgca
    20881 tcatagagat gcagtttggc ataacgggcg acgatgtttc ctgcctgaag tgccaccagc
    20941 atattccatg cgcgccccgg cgttgaagga acatgaatcg tcagaattgt cgtcatcatg
    21001 ttacgtttac tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat
    21061 ttaaccgata gatctgcatc atgatcatcg cgtgccagca atgcttccgg caggacaaac
    21121 agcgatacgt cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt
    21181 tcccacacag atgtaacagc aaactgtcct gcagcaacca gcattgccac ctcctgtcag
    21241 agtcgtaaaa ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa
    21301 atagcaggat tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg
    21361 aagcgtggcg agatggctgt taagtatgcg atttaaccca ctgcatcagg cgattccgtt
    21421 ggggacgctg acagcaaacc tgattggggc attcatcata ggaatgggat tcgcatggtt
    21481 cagcaggatg acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg
    21541 cggtctaaca accttctcaa cattttcggc agaagtggtg tttttgttac aagagggtcg
    21601 ctttggctgg gcattactga acgttttcgt caaccttctg gggtcttttg ccatgacagc
    21661 actggctttc tggctgtttt cggcctcaac cgcacactaa agacccaaaa aaaacccgct
    21721 gattaagcgg gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag
    21781 aagggccttt ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt
    21841 taaaaccatt agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg
    21901 gagtaatgaa accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag
    21961 acatcaaaat tacctttaca tgaaaaatag acacaaatgc tgcgtcggtt accagtacac
    22021 caattgtggt acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt
    22081 tttgatgtta cttattttag ctattgattt taaagaagtt actaaaactt catttgtctc
    22141 aaaactgaaa gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt
    22201 aggtcatctg aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga
    22261 gagggatgta attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat
    22321 gaaaattttc atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt
    22381 cccatttgtt ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt
    22441 catcccaacg cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt
    22501 tttctttgtc gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat
    22561 gttcaggctg ttgccaggtt ggtgcaatat tttctctaaa cgttgtcatc cactcatctg
    22621 cgttagcaaa aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga
    22681 catatttact cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat
    22741 agcaaagcta caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct
    22801 gctctattta tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc
    22861 gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa
    22921 tgcaatcaag gaaaaattat tttatttttt aacgagatac aacaatcacc ttaacaaagt
    22981 atataatata tatcgtcaac ccatgctcat attagattat tttttgagcg cacagccgca
    23041 gctaacacca ttgcaattaa caaatttgca tcaatccacc atcaatttgc acacattatt
    23101 atgtgataat tgccaaccgc taaatatgcg ttttgttatc tatgtataaa aacagtaact
    23161 tcaatgtctt aatggcagtt tttcttgatt ttaatcagca ttcatcgcca atttattggg
    23221 catatttttt ccttaagctt taggaatttt ttattattta ctttggggcc tggagacagg
    23281 aaaaattatg ctgacattca ttgaactcct tattggggtt gtggttattg tgggtgtagc
    23341 tcgctacatc attaaagggt attccgccac tggtgtgtta tttgtcggtg gcctgttatt
    23401 gctgattatc agtgccatta tggggcacaa agtgttaccg tccagccagg cttcaacagg
    23461 ctacagcgcc acggatatcg ttgaatacgt taaaatatta ctaatgagcc gcggcggcga
    23521 cctcggcatg atgattatga tgctgtgtgg atttgccgct tacatgaccc atatcggcgc
    23581 gaatgatatg gtggtcaagc tggcgtcaaa accattgcag tatattaact ccccttacct
    23641 gctgatgatt gccgcctatt ttgtcgcctg tctaatgtct ctggccgtct cttccgcaac
    23701 cggtctgggt gttttgctga tggcaaccct atttccggtg atggtaaacg ttggtatcag
    23761 tcgtggcgca gctgctgcca tttgtgcctc cccggcggcg attattctcg caccgacttc
    23821 aggggatgtg gtgctggcgg cgcaagcttc cgaaatgtcg ctgattgact tcgccttcaa
    23881 aacgacgctg cctatctcaa ttgctgcaat tatcggcatg gcgatcgccc acttcttctg
    23941 gcaacgttat ctggataaaa aagagcacat ctctcatgaa atgttagatg tcagtgaaat
    24001 caccaccact gctcctgcgt tttatgccat tttgccgttc acgccgatca tcggtgtact
    24061 gatttttgac ggtaaatggg gtccgcaatt acacatcatc actattctgg tgatttgtat
    24121 gctgattgcc tccattctgg agttcctccg cagctttaat acccagaaag ttttctctgg
    24181 tctggaagtg gcttatcgcg ggatggcaga tgcgtttgct aacgtggtga tgctgctggt
    24241 tgccgctggg gtattcgctc aggggcttag caccatcggc tttattcaaa gtctgatttc
    24301 tatcgctacc tcgtttggtt cggcgagtat catcctgatg ctggtattgg tgattctgac
    24361 aatgctggcg gcagtcacga ccggttcagg caatgcgccg ttttatgcgt ttgttgagat
    24421 gatcccgaaa ctggcgcact cttccggcat taacccggcg tatttgacta tcccgatgct
    24481 gcaggcgtca aaccttggcc gtaccctttc gcccgtttct ggcgtagtcg ttgcggttgc
    24541 cgggatggcg aagatctcgc cgtttgaagt cgtaaaacgc acctcggtac cggtgcttgt
    24601 tggtttggtg attgttatcg ttgctacaga gctgatggtg ccaggaacag cagcagcggt
    24661 cacaggcaag taaatagtaa tgccggaggc aagtttcctc cggctttatc atgtcaccca
    24721 ctgtggtata tgcgttgtgg tctgccaact ttgccgtgaa caatttcagc aataatcaga
    24781 tggcggctgg cgcaatattc aagataacgt ctggcagacg tgcggctgat ggtcagtgcc
    24841 tgcgccaccg tttctgccgt atgttgcaca ccaggctctt taaacagttt tcgcaccgcg
    24901 tttagcgtca agggatcaat gccggtcggc agttcgtctt taggttcacc gcgagcatag
    24961 gcattaaaca tctcatcaat ttgcttctgg ctggcgctat caatactttc cagcatatgt
    25021 ttacgctggc ggaaacgggt tagcgtttgc cccagccgtt cataggcaat gggcttaatg
    25081 agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc gctggctgca
    25141 gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcaa taaattaatc
    25201 cctctgccgt caggaagata gttatcgagc aagattagcc ccggcttaaa acgctcgatc
    25261 atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc gggaatgtga
    25321 cgaatatatt ccgcatgcat ctctgccagc ggcgtttcgt cctcaacgat caatagggtt
    25381 aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg taccgcaggg
    25441 atcattatct tcgagagtga taacaccacc gcagcgcgtt acatagctgg caatcaagta
    25501 caacccaatg ccatgttcac cgggctcgtc agcacgcgta ctgaccccct gctcaaatat
    25561 tttgtctcgt agagactctg gaacgccgca gccctgatcg gcgacttcaa tcaccacatc
    25621 atcgccttca tcgctgagga ataattcaac gatcttgttt ccttcatcgc tacgcaggct
    25681 ggcttcgaag gcgttatcaa gtaaattgcc cacaatggct gcaaactcgg tgctatccag
    25741 tcctggcagc agttgcgaaa gctggctacc ggggacaatg atcattttta gccccagttc
    25801 ccgggcgcgc tgcactttac caaaaagcag ccccgccacc tagcgatcgg caaacgcctc
    25861 gcgcaggctg tcaataagct gttgctgggc ctgagactcc ccctgcacca tcgccagcac
    25921 gcgatcatac tctttcatct gcaacagacc attgagcgtc gacatccaat tgagatgctc
    25981 gtgtcgcaat gtacgaaggc tctcaacgta ttgttttatt tgcatcagtt gcgcattgag
    26041 ggtggaaatt tcgtctttac tgcgaaagct gataatggcc cccagcaaat catcacctga
    26101 acgaatagct tcccgattgg caataacgct cagaccgtta aagttagcca ccatatcctg
    26161 acgtttttca tcaatctgtt cggtaaagaa atcggcgggc ctgaccactt cagcaatggg
    26221 tttacccaac cattgccgtc cgggggaact cagccccagc atctttcttg cgttacgatt
    26281 gatggcggta atgtaaccgt gcggatccac cgcaatcagc ccttcataaa ccgaactaaa
    26341 cagcgcctct tgctgacgga ccacgcgtgc gatttgcttt ggctccatgc ccatcatctg
    26401 ccgacggata tgcgcggcca ggaaccacga cagcaacatc agaatcccta acagcacgac
    26461 aaacacacct gccatcggta ataaaaactc agcccgccag ctatcgattt tactcaccag
    26521 gtagccaatc gacaccacgc cgatgacttt tccatcgtca tcaaagattg gcgttttggc
    26581 gcgcatcgcc atccccattg accctttccc ggtaatgaag tagctctccc ctttctccag
    26641 cgcgccctgt gtttggtgaa ctgcatagga taaccaattt tctccggatt aggatggtaa
    26701 aggcggatcg agtgccggtc gccatcacca cataatcaaa atcggtatct ctttgtaatt
    26761 tgttagcgat ggtcgccagc cgtttgtagt cacgcgtttt caccgcagag atgacactgt
    26821 cattggaggc aataattttc gcctgattca ttgccatgtc gcgtacatga agcgttaaat
    26881 agtcctcaaa actggccgta aaatattgcg ccaaggctgc aatgacaaat attgagaaca
    26941 ccagaatcag caaaaagata cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt
    27001 tattctcgtt aagctgcaac attgttttcc ttgcgggtta tcccgagcct ctttgacaga
    27061 tgtaattaaa atgttgaatc aaaatacata gatattaatt atgatcgtgg caaattttat
    27121 tcattaaatc aattaaatca cttaaacctg caggtcattt cgtaattaca ttaattaaaa
    27181 aacttaaacc attaaataaa taaaaatttc acgccgaaat actccttagg atgtatagcg
    27241 aaaggagaaa aagatatacc tcgatcaccc ccctttctcc caagtgaaaa taaaaggtta
    27301 tcagtttgca acattgaaca acattcgttg caaatcgata acaacatgca ccttcaggat
    27361 actatttatt atgttcggca atgatatttt cacccgcgta aaacgttcag aaaataaaaa
    27421 aatggcggaa atcgcccaat tcctgcatga aaatgatttg agcgttgaca ccacagtcga
    27481 agtatttatt accgtaaccc gcgatgaaaa gcttatcgcg tgcggtggaa ttgccggaaa
    27541 tattattaaa tgcgttgcta tcagtgaatc cgttcgcggt gaaggactgg cgctgacatt
    27601 agccactgaa ctgataaacc tcgcctatga gcggcacagc acgcatctgt ttatttatac
    27661 caaaaccgaa tacgaggcgc tgttccgcca gtgcggtttt tccacgctga ccagcgtacc
    27721 cggcgtgatg gtgctgatgg aaaacagcgc cacgcgactg aaacgctatg ccgaatcgct
    27781 gaaaaaattt cgtcatccag ggaacaagat tggctgcatt gtgatgaacg ccaatccctt
    27841 tacgaatggt caccgttatc tgattcaaca agctgcggca cagtgcgact ggttgcatct
    27901 gtttttagtc aaagaagatt cttcacgctt cccctatgaa gaccggctgg atctggtgtt
    27961 aaaaggcacc gccgatattc cacgcctgac tgtgcatcgc ggctccgaat acatcatctc
    28021 ccgcgctacg ttcccttgct acttcattaa agaacagagc gtcattaacc attgttacac
    28081 cgaaattgat ctgaagattt tccgtcagta cctcgctccc gcactgggtg taactcaccg
    28141 ctttgtcggt actgaaccct tttgtcgcgt taccgcccag tacaaccagg atatgcgcta
    28201 ctggctgaaa acgccgacta tctccgcacc gcccatcgaa ctggttgaaa ttgagcggct
    28261 gcgttaccag gagatgccga tatccgcttc ccgggtacgt caactgctgg cgaaaaacga
    28321 tctcacggct atcgcgccgc tggtccctgc agtcacgctg cattatttgc agaacctgct
    28381 tgagcactcc cgccaggacg cggcagctcg tcaaaagacc cccgcatgag aaacaggtga
    28441 aaaatgaaaa taaaccagcc cgccgttgca ggcacccttg agtctgggga tgtgatgata
    28501 cgcatcgccc cactcgatac gcaggatatc gacctgcaaa tcaacagcag cgttgagaaa
    28561 cagtttggcg atgcaattcg caccaccatt ctggacgttc tcgcccgcta caacgtgcgc
    28621 ggcgtacagc tgaatgtcga tgacaaaggc acactggact gcattttacg tgcacgactg
    28681 gaagccctgc tggcacgcgc cagcggtatc ccgactctgc catgggagga ttgccaatga
    28741 tttccgcttc gctgcaacaa cgtaaaactc gcacccgccg cagcatgttg tttgtgcctg
    28801 gtgccaatgc cgcgatggtc agcaactcct tcatctaccc ggctgatgcc ctgatgtttg
    28861 acctcgaaga ctccgtggca ttacgtgaaa aagacaccgc ccgccgcatg gtttatcacg
    28921 cgctgcaaca tccgctgtat cgcgatattg aaaccattgt gcgtgtcaac gcgctggatt
    28981 ccgaatgggg tgttaacgac ctggaagccg tcgttcgcgg tggtgcggac gttgtgcgtc
    29041 tgccgaaaac cgataccgct caggatgttc tggatattga aaaagagatc ctgcgtatcg
    29101 aaaaagcctg tggtcgtgaa cccggcagca ccggcctgct ggcggcgatt gaatctccgc
    29161 tgggcattac ccgcgcagtg gaaatcgctc acgcttccga gcgtttgatc ggtatcgccc
    29221 tcggtgcaga agactatgtg cgcaacctgc gtacagaacg ctccccggaa ggaactgaac
    29281 tgctgttcgc acgctgttcc attttgcagg ccgagcgctc tgcgggtatt caggcgttcg
    29341 ataccgtcta ttccgacgct aacaacgaag ccggattcct gcaagaagcc gcccacatca
    29401 aacagctggg ctttgacggc aaatcgctga tcaacccgcg tcagattgat ctgctgcaca
    29461 acctctacgc accgacccag aaagaagtgg atcacgcccg ccgcgtcgta gaagccgctg
    29521 aagccgccgc tcgcgaaggc ctcggcgtgg tttccctgaa cggcaagatg gtggacggtc
    29581 cggttatcga tcgcgcccgt ctggtgctct cccgtgcaga actttccggc atccgcgaag
    29641 aataaggcaa tcaaaatgac gcagaaaatt gaacaatctc aacgacaaga acgggtagcg
    29701 gcctggaatc gtcgcgctga atgcgatctt gccgctttcc agaactcacc aaagcaaacc
    29761 taccaggctg aaaaagcgcg cgatcgcaaa ctgtgtgcca acctggaaga agcgattcat
    29821 cgctctggtt tacaggacgg catgacggtt tccttccatc acgctttccg tggcggtgac
    29881 ctgaccgtca atatggtgat ggacgtcatc gcgaagatgg gctttaaaaa cctgactctg
    29941 gcctccagct ccctgagtga ttgccatgcg ccgctggtag aacacattcg ccagggcgtg
    30001 gttacccgca tttatacctc tggcctgcgc ggtccactgg cggaagaaat ctcccgtggt
    30061 ctgctggcgg aaccggtgca aatccactct cacggcggac gtgtgcatct ggtacagagc
    30121 ggcgaactga atatcgacgt ggctttcctc ggcgtcccat cctgtgatga attcggtaat
    30181 gccaacggtt acaccggtaa agcctgctgt ggctccctcg gctatgcaat ggttgatgcc
    30241 gacaacgcaa aacaggtcgt gatgcttacc gaagaactgc tgccttatcc gcataatccg
    30301 gcaagcattg agcaagagca ggttgatttg atcgtcaaag ttgatcgcgt tggcgatgct
    30361 gcaaaaatcg gcgctggtgc gacccgtatg accactaacc cgcgcgaact gcttattgcc
    30421 cgtagcgctg cggatgtgat tgtcaactct ggctacttca aagaaggttt ctccatgcaa
    30481 accggcaccg gcggcgcttc gctggcggta acccgtttcc tggaagacaa aatgcgtagc
    30541 cgcgatattc gcgccgactt cgccctcggc ggtattactg caacgatggt tgatctgcac
    30601 gaaaaaggtc tgatccgcaa actgctggat gtgcagagct ttgacagcca tgctgcgcaa
    30661 tcgctggccc gtaaccccaa tcacatcgaa atcagcgcca accagtacgc taactggggt
    30721 tcgaaaggcg catcggttga tcgtctcgac gtggtggtac tgagcgcgct ggaaattgac
    30781 acccagttca acgttaacgt gctgaccggc tctgacggcg tactgcgtgg tgcttccggt
    30841 ggtcactgcg ataccgcgat tgcctctgcg ctttccatca tcgtcgcgcc gctggtacgc
    30901 ggtcgtattc cgactctggt ggataacgtg ctgacctgca tcaccccggg ctccagtgtc
    30961 gatattctgg tcacagacca cggtatcgca gttaacccgg cacgtccgga gctggcagaa
    31021 cgtctgcagg aagcgggcat taaagtggtt tccattgagt ggctgcgtga acgtgcgcgt
    31081 ctgctgaccg gtgaaccaca gccgattgaa ttcaccgacc gcgtcgttgc cgttgtgcgt
    31141 taccgcgatg gctcggtgat cgatgttgta catcaggtga aggaataagc catgcacctg
    31201 cttcctgaac tcgccagcca ccatgcggtg tcaattcccg agctgctcgt cagccgggat
    31261 gaaaggcaag cacggcaaca cgtctggctc aagcgccatc ctgttccact ggtctccttt
    31321 accgtggttg cgcctaggcc gattaaagac agtgaggtca cacgccgaat ttttaatcat
    31381 ggcgtgacag ccttgcgtgc cttagccgca aaacagggct ggcaaattca ggagcaggct
    31441 gcactggttt ccgccagcgg gccggagggt atgttgagca ttgccgcccc ggctcgcgac
    31501 ctcaagctcg ccaccattga gcttgaacat agtcatcctc tcgggcggtt atgggatatc
    31561 gatgtcctga cgcccgaagg cgaaattctc tcccgccgcg actattcact gccgcctcgc
    31621 cgctgcctgt tgtgcgagca aagcgcagcc gtctgcgcgc gtggaaaacc catcaactga
    31681 ccgatttact caaccgcatg gaggcactgc tgaacgatgt cgatgcctgc aacgtcaact
    31741 aaaaccacaa agcttgcgac gtcattaatc gatgagtacg ccctgctggg ctggcgcgcc
    31801 atgctgactg aagtcaatct gtcaccgaaa ccaggtctcg tggatcgcat taactgcggt
    31861 gcgcataaag atatggcgct ggaagatttc caccgcagcg cgctggcgat tcagggctgg
    31921 ctaccccgtt tcattgaatt tggtgcctgt agtgcggaaa tggcaccaga agcggtactc
    31981 cacggattac gcccaattgg tatggcttgc aaaggtgata tgttccgcgc caccgcgggc
    32041 gtaaacacgc ataagggcag cattttttct ttagggctac tgtgtgcggc aattggccgt
    32101 ttgcttcagc tcaaccagtc gctaacgcca acaaccgttt gttctacggc ggcaagtttc
    32161 tgccgtggcc tgaccgatcg cgaactgcgt accaataatt cacaactgac ggcaggtcaa
    32221 cggttgtacc agcagcttgc ccttaccggc gcacgcggtg aagccgaagc gggttatcca
    32281 ctggtgatca accacgcctt gccgcattac ctcactctgc tggatcaggg gttagatcct
    32341 gaactggcat tgctcgatac cttgctccta ctgatggcga tcaacggcga taccaacgtt
    32401 gcttcgcgcg gtggcgaggg ggcctgcgct ggctacagcg cgaggcgcaa acattattgc
    32461 aaaaaggggg cattcgaacc cccgccgatc tcgattatct ccggcagttc gacagggagt
    32521 gtatcgaacg aaatctcagt ccaggcggca gtgccgacct gctgatcctt acctggtttt
    32581 tagcacagat ttaattattt aagcacttga taaatttgga aatattaatt ttcggagaac
    32641 ccgtatgtct ttagcaaaag ataatatatg gaaactattg gccccactgg tggtgatggg
    32701 tgtcatgttt cttatccctg tccccaacgg tatgccacca caggcatggc attacttcgc
    32761 tgtgtttgtg gcaatgattg tcggcatgat cctcgagcca attccggcaa cagcgatcag
    32821 ttttattgcg gttactattt gcgttattgg cagtaattac ctgctctttg atgccaaaga
    32881 attagctgac ccagcgttta atgcgcaaaa acaggcgctg aaatggggtc tggctggttt
    32941 ttccagcacc acggtatggc tggtatttgg cgcatttatt tttgcattag ggtatgaagt
    33001 ttccgggtta ggtcgtcgca ttgccctttt cctggtgaaa ttcatgggca aacgcacgct
    33061 gacgttgggt tatgcgattg tcattatcga cattctgctg gcgccgttta caccgtccaa
    33121 caccgcgcgt accgggggta cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa
    33181 atcattcccc aacgatccgt ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt
    33241 cattagtacc agtctgagtt cgtccatgtt tgtcaccggt gcggcaccaa acgtgctggg
    33301 tctggagttc gtcagcaaaa ttgccggtat ccagattagc tggttgcagt ggttcctctg
    33361 cttcctgccg gttggggtta tcttgcttat cattgcgccg tggctttcct acgtgctgta
    33421 caaaccggaa atcacacaca gtgaagaagt ggcaacctgg gcgggtgatg aactgaaaac
    33481 catgggtgcg ctgacacgca gagagtggac actgattggc cttgtattgc tcagcttagg
    33541 tttgtgggtt tttggcagtg aagtcattaa tgctactgca gttggtctgc tggcagtttc
    33601 gctaatgctg gccctgcacg ttgtgccttg gaaagatatt acccgctata acagcgcatg
    33661 gaacacgctg gtcaacctgg caactctggt tgtgatggct aacggcctga ctcgttctgg
    33721 ctttattgac tggttcgccg gtaccatgag tacgcacctg gaaggattct caccaaacgc
    33781 aacggtgatt gtactggttc tggtgttcta ctttgcacac tacctgtttg ccagcctgtc
    33841 tgcgcacacc gcaaccatgc tgccggttat tctggccgtc ggtaaaggta ttccgggcgt
    33901 accaatggaa caactgtgta tcctgctggt gctgtctatc ggtatcatgg gctgtctgac
    33961 gccctatgca accggtcctg gggtgattat ttacggctgt ggctatgtga aatcaaaaga
    34021 ttactggcgt cttggcgcaa tcttcggggt gatttatatc tctatgttgc tgttggttgg
    34081 ctggccgatt ctcgccatgt ggaactaata cgttcgccaa acgctgaata aatcatcacg
    34141 cccgccaggt aagcccacct ggcgggcgtg atgatttaat agatagtatt cagtgtatgt
    34201 atgagttcca cacccattat gaaagcattc tggcgtaacg ccgcgttgct cgcggtttct
    34261 ctgcttccct tctcttctgc caacgccgta gcgttgcagg caaaacagta tggcgatttt
    34321 gatcgctatg tcctggccct ctcctggcaa accggatttt gccagagtca acacgatcga
    34381 aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa cgaccaacaa agctgatttt
    34441 ctgaccgtac atggtctgtg gccaggattg cctaaatcgg ttgctgcccg tggtgttgat
    34501 gaacgccgct ggatgcgctt cggttgcgct actcgcccaa tcccgaatct accagaagcg
    34561 cacgccagcc ggatgtgttc atcgccggaa accgggttat cactggaaac ggccgctaaa
    34621 ctaagtgaag tcatgccagg agctggcgga cgttcctgcc tggaacgcta cgaatatgcc
    34681 aaacacggtg cctgctttgg ttttgatccg gacgcatact tcggtacgat ggtacgcctg
    34741 aatcaagaaa ttaaagagag cgaagccgga aaattccttg cggataatta cggtaaaaca
    34801 gtgagccgcc gtgactttga cgccgccttt gccaaaagct ggggaaaaga gaacgtgaaa
    34861 gcagttaagc taacgtgcca gggtaaccct gcgtatttga ctgaaattca gatctcgatc
    34921 aaagctgacg ccatcaacgc tccgctttct gcaaactcat tcttgccaca accacaccca
    34981 agcaactgtg gcaaaacctt tgtgattgat aaagtgggtt attaatcatc tgatttacaa
    35041 aacacgctaa ttcgtcttcc ataaaccgtg acgattaaga attatacata atgcgctgca
    35101 ttgaggaatt ccctgaatgt gacgcaaatc acttcaaaga gtgagcaaca gtcagtatca
    35161 tcatgtgagt taaaccctcg ccgcctgacg gtgagggttt tcttttgggt atgtttatct
    35221 gcgacactcg cagtaccgac gacatggagt aaaaatgtcc agaccaacta tcatcattaa
    35281 cgacctggat gccgaacgca tcgatattct gctggagcaa cccgcctatg ctggtttgcc
    35341 aatcgccgac gcgttaaacg cagagttgga tcgcgcccaa atgtgttcgc cagaagagat
    35401 gccacacgac gtggtgacaa tgaacagccg ggttaaattc cgcaatctta gcgatggcga
    35461 agtgcgtgtg cgcacgctgg tgtatccggc aaaaatgacc gatagcaata ctcagctttc
    35521 cgttatggct ccggtaggtg ccgcactgct ggggctgcgc gttggcgatt ccattcactg
    35581 ggaacttccg ggcggcgttg caacccacct tgaagtgctg gaactcgaat accagccaga
    35641 agctgctggc gactacctgc tttaatccca tctgcacaag cgataactcg ctttgcccag
    35701 aggatgcact cgcatcctct ttacgcaaaa tcagcaattt ttcctggttc tcactgttca
    35761 cttttgctgc aaaattattc cgtttcgttt gtgggaataa ccatggcaga agcattttac
    35821 atcctgatag gatttctgat tatggcggcg attatcgtca tggctgtcct ttacctcgaa
    35881 aaccactcct gacgatcata ttgttccccc cggcatcgca ttcaccagac ctgaaactgc
    35941 cttctgcgcc tcctctgcgc tttgtgcacc gggtaccaga atcaccttac ggcactcctc
    36001 ttcacgtttt tcgaaaatct catatccccg ggcggcctct tcaaacggca tatagtgggt
    36061 aacaatttct tctggtttca gtaatccttt ctcaattaac ggtaataatt ctcccagcca
    36121 tgcgtgaacg tgggtctgtc ccattttaaa cgtcaaccct ttatcaaagg cgtcaccaaa
    36181 caggaaaccg tgaataaatc cagcgtagac gcccggtacg ctaacaatgc cgccacgcct
    36241 gaccgccgca atacactggc gcaacgcttt accgctgctg ccctccagtt tcaggttagt
    36301 cagcaccgtt tccgtggtgc tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc
    36361 cacgccccgg tgacctgccg tttgttcaat aattgactgt gccggatcgc tatcttcatc
    36421 gaaattaatc gggatcgcgc cgtagcggtc agcggcgaaa ctcaagcggt agggatgatg
    36481 atcaacaaca aaaatctgtt ccgcaccgag caaccgtgca caggcgattg tcaacaatcc
    36541 cactggaccg gcaccataga ctgcaacgct tgaaccttgt tggatctgcg cattttttgc
    36601 tgcctgccat gccgttggca gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa
    36661 aggcggtact ttaaacggcc ccacattccc tttagggacg cggacatatt ccgcctgtcc
    36721 accaggaacg ccaccataca ggtgactata accaaacaat gccgcgggag ctggtatctg
    36781 ttttttattg agcgcagcgc ctttacccgc attggtattt tcgcaggcgg catattgctg
    36841 cattcgacag aaaaaacagt cgccacaagc aatgacaaac ggaattacca cccggtcgcc
    36901 tttttgcaaa ttttttacgt cctttccggt ttcaacgact tcccccataa attcatgacc
    36961 aaaaatatcg ccatgtttaa cctggggtat tttgcctcga taaagatgga gatcagagcc
    37021 acagatcgcc gttgccgtaa tacgcagaat aatatcatct gcctgttcaa tgcccggatc
    37081 gggaacattt tctacctgaa cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc
    37141 tccagagaca gttcaaaaaa gagtcagtct ttaagggtag ctatcacagc gggaaaacgg
    37201 gcggtaccgc aggttttggg agaattcctg gtgttcagtg agaatatatt aatcacagaa
    37261 accgttcgta aggaaaaaga accagacaaa tatcagggat aaatattata atcatggcga
    37321 atgatttgtt catgattaac agggagaaag ggttatgtat aagacaatca ttatgccagt
    37381 tgatgtattt gaaatggaat tgagcgacaa agctgttcgc cacgctgaat tcctcaccca
    37441 ggatgacgga gttattcatc tacttcacgt actacccggg tcagccagcc tgagcctgga
    37501 ccgttttgcc gctgatgtgc gtcgttttga agagcatctg caacatgaag cagaagaacg
    37561 tctgcaaacg atggtcagcc acttcaccat cgatccttcc cgcattaaac aacatgtccg
    37621 ttttggtagc gtgcgggatg aagtcaatga gttggcagaa gaactggggg ctgatgttgt
    37681 agttatcggt tctcgcaacc catcgatttc gacccatctg ttaggttcta acgcctcgag
    37741 cgtgatccgc cacgccaatc tgcctgtact ggtcgttcgc taacagataa aagtatttat
    37801 gccgccctgc ctggaacatc ccggcagggc tttttaatgc cataaaaaag ccgctaggtt
    37861 tgaccccggc ggctaagcaa ttgcaggtga atcttacttc ttcttatgca gttttggtgc
    37921 gaatcaggta gtcaaaagca ctcagagagg ctttggcacc ttcgccagtg gcgatgatga
    37981 tctgcttgta cggaaccgtc gtacagtcac ccgctgcgaa tacgcctttg acgttggttt
    38041 cgcatttcgc atcaatgata atctcaccca tgcggttacg ttcgactgcg ccttcgagcc
    38101 agttggtgtt cggcagcaga ccaatctgca cgaaaatacc ggccagttcg atgttgtgaa
    38161 tatcgccgct gacacgatct cgatattcca gaccaacgac tttgctgccg tcgcctttca
    38221 cttccgtggt ttgcgcattc agaataatgt cgacgttttt caggctgcgc agtttgtcct
    38281 gcagaacctg gtcggctttc atttctggcg caaattccag cagcgttacg tgctcaacga
    38341 tacccgccag gtcaattgcc gcttccacgc cggagttacc gccgccgata accgctacgc
    38401 gtttaccttt aaacagcggg ccgtcgcagt gcgggcagta ggtcacgcct ttggtgcgat
    38461 actgatcttc gcccggaacg ttcatgttgc gccattttgc accggtcgcc acgataatgc
    38521 tgcgtgcttt cagtaccgcg ccagaagctg tttcaatctg atgcagacca ccttcaactg
    38581 ctgctgggat cagtttgctg gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa
    38641 cgtgaacttt cagtgcgcct gccagcttct gcccttcagt cttcggtaca gaaatgtagt
    38701 tttcgatatc aacggtatcg aggatctgac caccaaaacg ttcgcccatc agaccggtac
    38761 ggatgccttt acgtgcggag taaattgctg ccgctgcacc cgccgggccg gaaccgacga
    38821 ttaatacgtc ataagcatca cgcttgttca gctcttctgc cgcacgtttt tccgcgcccg
    38881 tatcaatttt ggcaacgatt tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt
    38941 ttacgaacac tgccggaacg cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc
    39001 cgccgtcaat tgcagtgtgc ttgatgcgcg ggttcagtac gctcatcagg ttcagcgcct
    39061 gcaccacgtc cgggcagtta tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac
    39121 cgtcaatatg gcgaatctgc tccagcagag actgcgcttc tttcgacgga tgaccaccgg
    39181 tccacagcaa cgccagtacc agcgaggtga actcgtggcc cagcggggag cctgcaaaac
    39241 gtggcccctg gttggaacct gggttggtga tcaggaaaga cggcttacgc accggcaagc
    39301 tgttatcttc tttaaaggtg actttgtctg acagttctgc gatttcagcc aacagttcct
    39361 tgatttctgc cgatttagcg ctgtcatcca gcgtggcaat taactcaaca ggcttggtca
    39421 atttctcaag gtaagccttg agttgagttt tcatatttgt gtcgagcatg tttatctcct
    39481 gggctttaaa catcatcatg caagcggcct ctacggctac ctggatgcag cttgcatcat
    39541 agtgcgggga agaaaatcgg gtgccgcagc acccgaagaa ttaggtgaat ttccgaagta
    39601 tttcgcgccg gagttggtag cgcgaaagac gaaggaaatt tagattttac caaccaggtc
    39661 cagagacgga gccagagttg cttcaccttc tttccattta gccgggcaaa cttcacctgg
    39721 gtgagaagct acgtactgtg ctgctttgat tttacgcagc aggtcagacg cgtcacggcc
    39781 aatgccttca gcggtaactt cgattgcctg gatgataccc tgcgggtcaa caacgaaggt
    39841 cgcacggtca gccagacctt catcttcacg catgttgtcg aagttacggg tcagggcgcc
    39901 agtcgggtcg ccgatcatcg catatttgat tttagcgatg gtttcagagc tgctgtgcca
    39961 tgctttgtgg gtgaagtgag tatcggtaga tactgcgtat acgtctacgc ccagtttctg
    40021 cagttcttcg tagtggtcag caacgtcacc cagttcggtc gggcatacga aagtaaagtc
    40081 agccgggtag aagaagaaga cgctccagcg gccttcggta tctttttcgg tgatttcgat
    40141 gaattcgccg tttttgaatg cctggttttt aaaaggttta attttggtgt taatcaagga
    40201 catctatact tcctccgtgt tttcgatgag atgtaaggta accaattttt gccgattcgg
    40261 ctaatgcgtt tccattatca aatcgataag ttttacctta caaccttcgt aagacaactt
    40321 tgtgaacttt gcaccgccag cggcgataaa aagtaaaatg gccccctttc caggtgaccg
    40381 tgtaatctga actacccttt ggtatcttca gcgcctgcct gcaggctgag gcgttgccct
    40441 tacaaatgca acaacgacat ggattacaac accctcataa acaaagggca atcacctgat
    40501 ctaagctctt acctatgaca gtgataggtt atgcctttta ctcgactttt gcactgactg
    40561 aaaaggacaa attaatgtta aaaaagatac ttttactggc tctgcttcct gcaatcgcct
    40621 tcgcagagga acttcctgct ccagtaaaag cgattgaaaa acagggcatt acaatcatca
    40681 aaacgttcga tgcccccgga ggaatgaaag gttatctcgg aaagtatcag gatatgggcg
    40741 tcaccatcta cctgactcca gatggtaagc acgctatctc tggttacatg tacaacgaga
    40801 aaggtgaaaa cctgagtaac acacttatcg aaaaagaaat ttacgcacca gccggacgcg
    40861 aaatgtggca acggatggaa caatcccact ggctcctcga cggtaaaaaa gatgcgccgg
    40921 tcattgtcta cgtcttcgcc gatccgttct gcccatattg taaacagttc tggcagcagg
    40981 c
//
LOCUS       DADLTD010000009        40425 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLTD010000009 DADLTD010000000
VERSION     DADLTD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.009"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(115..2436)
                     /locus_tag="JN701_000367"
     CDS             complement(115..2436)
                     /locus_tag="JN701_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY7362911.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2533..3045)
                     /gene="greB"
                     /locus_tag="JN701_000368"
     CDS             complement(2533..3045)
                     /gene="greB"
                     /locus_tag="JN701_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY7362912.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            3237..3956
                     /gene="ompR"
                     /locus_tag="JN701_000369"
     CDS             3237..3956
                     /gene="ompR"
                     /locus_tag="JN701_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY7362913.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3953..5305
                     /gene="envZ"
                     /locus_tag="JN701_000370"
     CDS             3953..5305
                     /gene="envZ"
                     /locus_tag="JN701_000370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY7362914.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5381..7003)
                     /gene="pckA"
                     /locus_tag="JN701_000371"
     CDS             complement(5381..7003)
                     /gene="pckA"
                     /locus_tag="JN701_000371"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY7362915.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7382..9106
                     /locus_tag="JN701_000372"
     CDS             7382..9106
                     /locus_tag="JN701_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY7362916.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9241..10119)
                     /gene="hslO"
                     /locus_tag="JN701_000373"
     CDS             complement(9241..10119)
                     /gene="hslO"
                     /locus_tag="JN701_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY7362917.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10144..10545)
                     /gene="hslR"
                     /locus_tag="JN701_000374"
     CDS             complement(10144..10545)
                     /gene="hslR"
                     /locus_tag="JN701_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY7362918.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10556..11224)
                     /gene="yrfG"
                     /locus_tag="JN701_000375"
     CDS             complement(10556..11224)
                     /gene="yrfG"
                     /locus_tag="JN701_000375"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY7362919.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11289..13424)
                     /gene="igaA"
                     /locus_tag="JN701_000376"
     CDS             complement(11289..13424)
                     /gene="igaA"
                     /locus_tag="JN701_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY7362920.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13744..14304
                     /gene="nudE"
                     /locus_tag="JN701_000377"
     CDS             13744..14304
                     /gene="nudE"
                     /locus_tag="JN701_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY7362921.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14469..17021)
                     /gene="mrcA"
                     /locus_tag="JN701_000378"
     CDS             complement(14469..17021)
                     /gene="mrcA"
                     /locus_tag="JN701_000378"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY7362922.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17141..17920
                     /gene="hofM"
                     /locus_tag="JN701_000379"
     CDS             17141..17920
                     /gene="hofM"
                     /locus_tag="JN701_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY7362923.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPSGDFAIALGLALGKAY"
     gene            17920..18459
                     /locus_tag="JN701_000380"
     CDS             17920..18459
                     /locus_tag="JN701_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HAY7362924.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18443..18883
                     /gene="hofO"
                     /locus_tag="JN701_000381"
     CDS             18443..18883
                     /gene="hofO"
                     /locus_tag="JN701_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY7362925.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18873..19277
                     /gene="hofP"
                     /locus_tag="JN701_000382"
     CDS             18873..19277
                     /gene="hofP"
                     /locus_tag="JN701_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY7362926.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19189..20427
                     /gene="hofQ"
                     /locus_tag="JN701_000383"
     CDS             19189..20427
                     /gene="hofQ"
                     /locus_tag="JN701_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY7362927.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20828..21349
                     /gene="aroK"
                     /locus_tag="JN701_000384"
     CDS             20828..21349
                     /gene="aroK"
                     /locus_tag="JN701_000384"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY7362928.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21406..22494
                     /gene="aroB"
                     /locus_tag="JN701_000385"
     CDS             21406..22494
                     /gene="aroB"
                     /locus_tag="JN701_000385"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY7362929.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22586..23872
                     /gene="damX"
                     /locus_tag="JN701_000386"
     CDS             22586..23872
                     /gene="damX"
                     /locus_tag="JN701_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY7362930.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23979..24815
                     /gene="dam"
                     /locus_tag="JN701_000387"
     CDS             23979..24815
                     /gene="dam"
                     /locus_tag="JN701_000387"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7362931.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24833..25510
                     /gene="rpe"
                     /locus_tag="JN701_000388"
     CDS             24833..25510
                     /gene="rpe"
                     /locus_tag="JN701_000388"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY7362932.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25503..26261
                     /gene="gph"
                     /locus_tag="JN701_000389"
     CDS             25503..26261
                     /gene="gph"
                     /locus_tag="JN701_000389"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY7362933.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26254..27258
                     /gene="trpS"
                     /locus_tag="JN701_000390"
     CDS             26254..27258
                     /gene="trpS"
                     /locus_tag="JN701_000390"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY7362934.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27527..28432
                     /locus_tag="JN701_000391"
     CDS             27527..28432
                     /locus_tag="JN701_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362935.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIVLE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28449..28811
                     /locus_tag="JN701_000392"
     CDS             28449..28811
                     /locus_tag="JN701_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7362936.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28895..30058
                     /locus_tag="JN701_000393"
     CDS             28895..30058
                     /locus_tag="JN701_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY7362937.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            30058..31284
                     /locus_tag="JN701_000394"
     CDS             30058..31284
                     /locus_tag="JN701_000394"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7362938.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31281..32159
                     /locus_tag="JN701_000395"
     CDS             31281..32159
                     /locus_tag="JN701_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY7362939.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRFHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32170..32523
                     /locus_tag="JN701_000396"
     CDS             32170..32523
                     /locus_tag="JN701_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY7362940.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32535..33839
                     /locus_tag="JN701_000397"
     CDS             32535..33839
                     /locus_tag="JN701_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362941.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33851..34936
                     /locus_tag="JN701_000398"
     CDS             33851..34936
                     /locus_tag="JN701_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7362942.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35088..35819)
                     /gene="phoB"
                     /locus_tag="JN701_000399"
     CDS             complement(35088..35819)
                     /gene="phoB"
                     /locus_tag="JN701_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY7362943.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35919..36704)
                     /gene="frlD"
                     /locus_tag="JN701_000400"
     CDS             complement(35919..36704)
                     /gene="frlD"
                     /locus_tag="JN701_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY7362944.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36701..37531)
                     /gene="frlC"
                     /locus_tag="JN701_000401"
     CDS             complement(36701..37531)
                     /gene="frlC"
                     /locus_tag="JN701_000401"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY7362945.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37581..38603)
                     /gene="frlB"
                     /locus_tag="JN701_000402"
     CDS             complement(37581..38603)
                     /gene="frlB"
                     /locus_tag="JN701_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY7362946.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38624..40012)
                     /gene="frlA"
                     /locus_tag="JN701_000403"
     CDS             complement(38624..40012)
                     /gene="frlA"
                     /locus_tag="JN701_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HAY7362947.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            complement(40257..40424)
                     /locus_tag="JN701_000404"
     CDS             complement(40257..40424)
                     /locus_tag="JN701_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY7362948.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         9348 a        10252 c        11117 g         9708 t
ORIGIN      
        1 tgtggaataa aaaggccgga gatattctcc ggcactttta tgtcaggcaa ggcgttcgtt
       61 gccagatgcg gcgtgaacgc cttatcgcgc aggtttaggg gccagtccgg gcgtttaacg
      121 ttttttgccc attgccgccg ccagcgcatc catcatcgcg ctattaccgg caggctgcgc
      181 ttcacgaccg cgtggtttgg ctgccgggcg gttgttttgc gggcgttcat taccgccgcc
      241 gcgacgggcg ttggtttcgc caggctgctc gtccagacgc atagtcaggg cgatacgttt
      301 acgctgaaga tccacttcca gcactttcac cttcacaatg tcgcccgctt tcaccacggt
      361 atgcggatct tccacaaact tgttcgacaa tgaagagatg tgaaccaggc cgtcctgatg
      421 cacgccgata tcgacaaacg cgccaaagtt ggtgacgttg gtcaccgcac cttcgaggat
      481 catacccggt tgcaggtcgt tcattgtctc gacgccatcg gcaaactgag cggttttaaa
      541 ttccggacgc ggatcgcgac ccggtttttc cagctctttg atgatgtcag ttactgtcgg
      601 cacaccgaat ttttcatcag taaagtcaga cgctttcagg ttacgcagtt cgctgctgtt
      661 acccatcaga tctttcagtg cctgctgtgt tgctgccaga atgcgttcca ccaccggata
      721 ggcttccggg tggacggtag aagcatccag cgggttatca ccgtggttaa tgcgcaagaa
      781 gcccgcgcac tgctcgaagg ctttcggccc cagacggctg actttcagca gttgctgacg
      841 gttctggaac tggccgttct catcgcgcca ggccacgatg ttttgcgcca tcatgcgcgt
      901 caggcccgcc acgcgggtta acagcggaac agaagcagtg ttgagatcga cgccaacggc
      961 gtttacgcag tcttctacta ctgcgtccag tttgcgggcc agttgcgtct ggctgacgtc
     1021 atgctgatac tgacctacgc cgatagattt cggatcgatt ttcaccagct ccgccagcgg
     1081 atcctgcaaa cggcgggcga tagacaccgc gccacgcagc gaaacgtcga gatccgggaa
     1141 ctcctgcgct gccagctcgg aagctgagta aaccgacgcg ccagcttcgc tgacgatcac
     1201 tttctgtgcg gtcactttcg ggaactgctt ctgcacgtcg agatagaaac gttcagtttc
     1261 gcgggaagct gtaccgttgc cgatcgccac cagttcaacg ttatgttttt cacacaaggc
     1321 agcaacggtc atcgctgctt ttgcggcttg tccggtgtgc gggtaaatgg tatcggtcgc
     1381 taccagtttg ccagtggcat cgaccaccgc cacttttacc ccagtacgca gacccggatc
     1441 gaggcccatc gttgcacgca gtccggcagg ggccgccatc agcagatcgt gcaggttacg
     1501 ggcaaagacg ttgattgctt catcttccgc gcgttcgcgc acggtaccca tcagttcggt
     1561 ttccagatgc atcagcacct tgatgcgcca ggtccagctc actacgcctt tgcgccagct
     1621 atccgccggg gcattgttca ggcgcaggcc aaggtgatcc atgatgattt gctcgcaata
     1681 gctctctttg ggcggctcat cgaactgcgg atcggcattc agcgaaagct ggagtacgcc
     1741 ttcgttacgc ccacggaaca tcgccagagc gcggtgagaa ggcaccgtgg acaacggttc
     1801 gtgatgatcg aaatagtcgc ggaatttcgc cccttcctct tctttaccgc tcaccaccgt
     1861 agaaaccaaa tgcgcgttct tccacagata atcacgcact ttcgccagca gcgcggcatc
     1921 ttcggcaaac cgttccatca ggatatagcg cgcgccatcc agcgcggctt tggtatctgc
     1981 cacgccttta tcggcatcaa catattgtgc agcggcgact tctggcgtgt gtgacggatc
     2041 gctccacagc aggtcagcca acggctcaag ccctgcttca atggcgattt gcccgcgggt
     2101 gcggcgttta ggtttgtagg gcaggtagag gtcttcgagt tcggttttgc ttagggtggc
     2161 gttgatggcc ttcgccagat catcggtgag tttgccttgc tcggaaatgg acttgaggat
     2221 cgcctgacgt ctctcttcca gctcgcgcag atagctcaga cgcgtttcca gattacgcag
     2281 ctgcgtgtca tccagaccgc cggtgatttc cttacgataa cgtgtgataa acggcacggt
     2341 attcccttcg tcaagcaggc gaacggcagc gtcaacctgt tccgggcgcg cctgaatttc
     2401 acccgcaata atgcggcaga acgaatcatt catcatggtt tgagttcatc ttttcggatc
     2461 aaaaatcagg ggatagttat acggactggc tggcaaaaat gccagccatc ggcaggaggt
     2521 taagactctt ccttacggtt tcacgtactc gatagcatta acataccagc tcgcttcccc
     2581 ggcaggggta ttcaccaccg ccagatcgcc gacttctttt ttcagcaatg cgcgggccat
     2641 cggggaatcg atagagatgt aatctttacg gccaaaaatt tcatcgtagc cgacaatacg
     2701 gaaacggtga gtcacgccat cgtcgttttc aatctccacc cacgcgccaa aaaagacttt
     2761 gccttcctgc tgaggggagt aatcgacgat tttgagattt tccatgcatt tagtgagata
     2821 gcgcacgcga cggtcgattt cacgcagacg ctttttatta tactgatagt cagcattttc
     2881 gctgcggtcg cccagacttg cggcccaggt cacctttttt gtgacctccg ggcgttcttc
     2941 acgccagaga taattaagct cttgtttgag tttttcatac ccttcccggg taaccagggg
     3001 cgttttcatc tcgttgattc cctttgtctg tttgataatg cgcacattgg gtataacgtg
     3061 atcatatcaa cagaatcaat aatgtttcgc cgaataaatt gtatacttaa gctgctgttt
     3121 aatatgcttt gtaacaattt aggctgaaat tcataccaga tttagctggt gacgaacgtg
     3181 agctttttta agaatacacg cttacaaatt gttgcgaacc tttgggagta caaacaatgc
     3241 aagagaacta caagattctg gtggtcgatg acgacatgcg cctgcgtgcg ctgctggaac
     3301 gttatctcac cgaacaaggc ttccaggttc gaagcgtcgc taatgcagaa cagatggatc
     3361 gcctgctgac tcgtgaatct ttccatctta tggtactgga tttaatgtta cctggtgaag
     3421 atggcttgtc gatttgccga cgtcttcgta gtcagagcaa cccgatgccg atcattatgg
     3481 tgacggcgaa aggggaagaa gtggaccgta tcgtaggcct ggagattggc gctgacgact
     3541 acattccaaa accgtttaac ccgcgtgaac tgctggcccg tattcgtgcg gtgctgcgtc
     3601 gtcaggcgaa cgaactgcca ggcgcaccgt cacaggaaga ggcggtaatt gctttcggta
     3661 agttcaaact taacctcggt acgcgcgaaa tgttccgcga agatgagccg atgccgctca
     3721 ccagcggtga gtttgcggta ctgaaggcac tggtcagcca tccgcgtgag ccgctctccc
     3781 gcgataagct gatgaacctt gcccgtggtc gtgaatattc cgcaatggaa cgctccatcg
     3841 acgtgcagat ttcgcgtctg cgccgcatgg tagaagaaga tccagcgcat ccgcgttaca
     3901 ttcagaccgt ttggggcctg ggctacgtct ttgtaccgga cggctctaaa gcatgaggcg
     3961 attgcgcttc tcgccacgaa gttcatttgc ccgtacgtta ttgctcatcg tcaccttgct
     4021 gttcgccagc ctggtgacga cttatctggt ggtgctgaac ttcgcgattt tgccgagcct
     4081 ccagcagttt aataaagtcc tcgcgtacga agtgcgtatg ttgatgaccg acaaactgca
     4141 actggaggac ggcacgcagt tggttgtgcc tcccgctttc cgtcgggaga tctaccgtga
     4201 gctggggatc tctctctact ccaacgaggc tgccgaagag gcaggtctgc gttgggcgca
     4261 acactatgaa ttcttaagcc atcagatggc acagcaactg ggcggcccga cggaagtgcg
     4321 cgttgaggtc aacaaaagtt cgcctgtcgt ctggctgaaa acctggctgt cgcccaatat
     4381 ctgggtacgc gtgccgctga ccgaaattca tcagggcgat ttctctccgc tgttccgcta
     4441 tacgctggcg attatgctat tggcgatagg cggggcgtgg ctgtttattc gtatccagaa
     4501 ccgaccgttg gtcgatctcg aacatgctgc tttgcaggtc ggtaaaggga ttattccacc
     4561 gccgctgcgt gaatatggcg cgtccgaggt acgttcggtt acccgcgcct ttaaccatat
     4621 ggcggctggt gttaagcaac tggcggatga ccgcacgctg ctgatggcgg gggtaagtca
     4681 tgacttacgc acgccgctga cgcgtattcg cctggcgacc gagatgatga gcgaacagga
     4741 tggctacctg gccgaatcga tcaataaaga tatcgaagag tgcaacgcca tcattgagca
     4801 gtttatcgac tacctgcgca ccgggcagga gatgccgatg gaaatggcgg atctcaacgc
     4861 agtactcggt gaggttattg ctgccgaaag tggctatgag cgggaaattg aaaccgcgct
     4921 ttaccccggc agcattgaag tgaaaatgca cccgctgtcg atcaaacgcg cggtggcgaa
     4981 tatggtggtc aacgccgccc gttacggcaa tggctggatc aaagtcagca gcgggacgga
     5041 gccgaatcgc gcctggttcc aggtggaaga tgacggtccg ggaattgcgc cggaacaacg
     5101 taagcacctg ttccagccgt ttgtccgcgg cgacagtgca cgcaccatta gcggcacggg
     5161 attagggctg gcaattgtgc agcgtatcgt ggataaccat aacgggatgc tggagcttgg
     5221 caccagcgag cggggcgggc tttccattcg cgcctggctg ccagtgccgg taacgcgggc
     5281 gcagggcacg acaaaagaag ggtaaataaa cgggaggcga aggtgcctcc cgttttgctt
     5341 tctataagat actggataga tattctccag cttcaaatca ttacagtttc ggaccagccg
     5401 ctaccagcgc ggcacccgca ggggtgtcgg tgtatttatc gaagttgtcg ataaacagtt
     5461 tcgccagggt ttcggctttt tcctgccact gttccggaga agcgtaggtg ttacgcggat
     5521 cgagaatctt cgtgtctacg cccggcagtt cggttgggat cgccaggtta aacatcggca
     5581 gagtgaaggt ttctgcatta tccagcgaac cgttgaggat ggcgtcgata atggcgcggg
     5641 tatctttaat cgagatacgt ttgcctgtgc cgttccagcc agtgttaacc agataagcct
     5701 gcgcgcccgc cgcctgcata cgtttcacca gcacttctgc gtactgagtc gggtgcagcg
     5761 acaggaatgc cgcgccgaag caagcggaga aggttggcgt cggttcggtg atgccacgct
     5821 cagtaccggc cagtttggcg gtgaagccag agaggaagtg atactgggtt tgatcggcag
     5881 tcaggcgaga aaccggcggc aacacgccga aagcatcagc agtcaggaag ataaccttag
     5941 tcgcgtggcc cgctttggaa accggcttaa cgatgttatc gatgtgatag attggataag
     6001 aaacgcgggt gttctcggtt tttgaaccat catcaaagtc gatagtgcca tcttcacgca
     6061 cggtgacgtt ttccagcagc gcatcacgac ggatagcgtt gtagatttca ggttccgctt
     6121 ctttcgacag cttgatagtt tttgcgtagc agccgccttc gaaattaaac acgccgtcat
     6181 cgtcccagcc gtgttcgtca tcgccaatca ggcggcgttt cgggtcggtg gaaagggtgg
     6241 ttttaccggt gccggaaagg ccgaagaaca ccgcaacatc gcctttctcg ccaacgttgg
     6301 cggagcagtg catagaagcg atacctttca gcggcagcag gtagttcatc atcgagaaca
     6361 tccctttctt catttcgccg ccgtaccagg tgccgccaat cagctgcatg cgctcggtca
     6421 ggttaaacgc cacgaagttt tcggagttca gaccctgttc tttccactgc gggttagtgc
     6481 acttcgcgcc gttcataacg ataaagtctg gtttgaaacc tgccagctct tcatcgctcg
     6541 ggcgaataaa catgtttttg acaaaatgcg cctgccaggc cacttcggtg atgaaacgga
     6601 cggaaagacg agtatccggg ttcgcaccac agaaagcgtc gacaacgaac agacgtttgc
     6661 cggaaagctg cctggtcacc aggcctttca gatgctgcca ggtttccgga gagagaggtt
     6721 tgttgtcgtt cttacctttg cctttgtctg cccaccagaa agtatcgcga gtggtatcgt
     6781 cacggacgat atacttatct tttggtgaac gaccggtgaa gatcccggta tcgacggcaa
     6841 cggcacccag attagttaac accccgcgct cataacctgt caggctcgga tcgagctctt
     6901 cctgatacag caggtcgtag cttgggttgt aaacgatatc atgtacgtca ctgataccat
     6961 aagcctcgag ttcttgcggg gtcaaaccat tgttaacgcg catttcactg ctccttagcc
     7021 aatatgtatt gcctgaatag taaagtcttt ttgggggtgt taaccgcgac aaggctcata
     7081 gatttacgta tctggagaaa ttcatatttg acaaaaaacc ccgtgattcc tgttacgaaa
     7141 cggttgctat tatcgcagaa atagcatttt aggtgtggaa aatgttctca aaaggttaaa
     7201 tcttggttta tatggcggga atattgtgag tggaatcgca ttccttaatg aaattgatgc
     7261 aaattcaatc ggtaaagtat cgattcacca cgctaacttt ttttgattat tttctgaaaa
     7321 aagcaaaata ctactgcccg gatgcgataa ttaacccacc ttttgattct ggataaccaa
     7381 catggataac gttgaacttt caccggcaac acgctggggg atgattgcta ccggattact
     7441 tcaggggctg gtttgctacc tgttgatcgc ctggcttgcc ggaaaaaatc acagttggat
     7501 tgtttatggc gtgcctgcga ccgttgcgtt ttcatccgtt ttactcttct ccgtgatctc
     7561 ttttaaacag aagcgccttt ggggatggct ggcgctggtg tttattgcca cgacagggat
     7621 gagcggttgg ctgaagtggc aaactgatgg catgaacccc tggagagctg aaaaggcgat
     7681 ttgggatttt ggctgctatc tgctgttgat ggcaatgttg ttgctaccgt ggatacaaca
     7741 aagccttcgt atccgcaatt gcagtagccg ctatagctac ttttaccagt cagtatggca
     7801 taacgtactt atattactgg tgatttttct cgccaatggc ctgacgtggc tggtgctttt
     7861 actgtggggt gagttgttta aacttgttgg catcaagttt ttcaatacgc tcttttttgc
     7921 aaccgactgg tttatgtatc ttacattagg tctggttacc gcactggcgg tgattctcgc
     7981 gcgcacacag tcacgtttaa tcgactctat tcaaaagttg ttcacgttaa tcgccacggg
     8041 gttgctgccg ttagtatcat tgctaaccct gatgtttatc atcactctgc cgtttacggg
     8101 cctgagcgcg atttctcgcc acatctccgc cgccgggttg ctgttaacgc tggccttttt
     8161 gcaattgatc ttaatggcta ttgtccgcga tccgcaaaaa gcgtcacttc cctggacggg
     8221 gccgttgcgt tgcctgatta aaaccgcttt gctggttgct ccgctgtatg tgttcgtcgc
     8281 cgcctgggcg ttatggctgc gggtcgctca gtacggctgg actgtcgacc gcttgcaggg
     8341 cgcgctggcg gtgctggtgt tactggtgtg gtcgctgggg tattttgtca gtatcgtctg
     8401 gcgtaatggg caaaatcccc ttgttcttca gggcaaagtg aaccttgcgg tttcgttatt
     8461 ggtgttggtg atactggtgc ttcttaattc gccggtgctg gacagtatgc gtattagcgt
     8521 gaacagtcat atggcgcgtt atcagagcgg caaaaacacg ccagaccagg taactatcta
     8581 catgctcgaa cagagcggtc gctatggacg tgcggcgctt gagtcgctga aaagcgatgc
     8641 cgggtttatg aaagacccga aacgcgcgcg ggatctgctg atggcgttag atggagagca
     8701 acatcttcag gaacaggtat cggaaaaagt attagccgaa aatgtgttaa ttgcccctgg
     8761 ttctgttaaa ccggatgtga cattctggtc ggccttaatc caggatcgct ataacgtgat
     8821 gacctgtatt gaaaaagacg cctgcgtcct ggtcgagcaa gatctgaata gtgatggtca
     8881 ggcggagcgg atcctgtttg cttttaatga tgacagagtc attgtctatg gctttgactc
     8941 agacagaaaa gaatgggacg cgcttgatat gagtttactt ccgaacgaaa taacgaaaga
     9001 aaaattactc acagctgcga aggatggaaa actggggacg aggccgaaag cgtggcgcga
     9061 tctgacggta gatggtgaaa cgctggaaat aaacttaagt aagtgacaac gcttttatcg
     9121 gatacgacgc tgtatctgac caaaataatt tgcgatttat ttcataggcg ctggtaagcg
     9181 cagcacatca ggccattaag ttgtaaaatc cggcagagcc ctctctgccg gacatactca
     9241 ttaatgaact tgcggatctg ccggagacgc gttgttgcgg atttcagcaa tatccatcgc
     9301 attgaacaga tagtggttac cgcagtaatc acaatgcatg tcaatttcgc catcttccgc
     9361 caggatgcta tcaacttctt catcaggcag cgttttcagc gcatcggcgc aatgttcacg
     9421 cgagcaggtg catttgaact ccacatcctg cggatcgtaa accgtcacct cttcttcgtg
     9481 atacaaacgc cacaacactt cgtttgccgg taaggtcagc agttcttcgg ttttgatggt
     9541 ttcggttagc gtcgccaggt ggtcaaagtc gtcctgctgg gcattttgcg caggcattac
     9601 ctgcaacaac ataccgcctg cagccggttt gccgtctacg tcgccggtgc gaataaacag
     9661 gcgcgtcggc agctgttcag aacgcataaa gtaatcttcc aggcaggccg ccagggtatc
     9721 accttccaga ccaactacgc cctgatagcg ttcgccttcg ctcggggtaa tggtgatcac
     9781 cacgtaacca ttgccgacca gcgttttcag gtcggcattt tctggaattt cgccctgcac
     9841 gcgcgccaca ccgcgcattt gctggttatt gttaccgtta ataaccgcca gattcatcgg
     9901 accgtcgccc tgcagctgta cggtgatatc accatcaaac ttcagcgtag cggttaacag
     9961 gctggtcgca accagcagtt ctgccagcac gtttttaacg ggctgcggat aatcgtggtt
    10021 ctcaaggatc tgttgcaggg tttccgaaac ggttaccagt tcgccgcgaa cggcaaaatt
    10081 ttcaaacaga tagcgatgta attggtcatg ttgcggcata atcatctctc ttgcaggtga
    10141 cagttattca ctgtcgccgt gtttaaatcg taacaggtcg cggcgctctt ttttgtccgg
    10201 gcgtcggtcc gggtgcggca tggttaaggc attaagttta cgtgccatcg ccattttttc
    10261 gcgtttctct acactttccg cagtctcttc atacagcaag gctgcctcgc tggcggggcg
    10321 acgctgttca gtaatcgcct ttacaatcac cgtgcgttcg tcatttccct ggcgcagagt
    10381 gagcgtggca ttcagctcga cgattttgct cggcttgctg cgctgcccgt tgtaatgcac
    10441 cttaccgcct tcaatcattt cacgggccag cgcgcgggtt ttataaaaac gggcagccca
    10501 tagccattta tccagtcgaa cctcaacagc aggtttctct ttcatggcgt ctccttcaca
    10561 ttagcgaggg gatcaggcgg cggtagtcat tcagtgacgg atggcgttga tactgtttct
    10621 cggcaatccc ggaatcagga ttagtcacgc cgaggcagta acgaatacca aattgcgcgg
    10681 cagcatcgag aatcgcttcg ctgtcatcaa taaacagcgt tctttcagct ttcagacccg
    10741 tagcttcggc caccgcatgc cataaccgct gatcctcttt cggataacca aatgtgtggg
    10801 tggaaagtaa taaatcaagg tgtgcgtcca gacccgtatg ctcaagtttt accgccaggt
    10861 tgtgcggatg agcattggtg agcaaaattc gctgcttacc gctggctttc agtgcctcaa
    10921 gaaacggaat ggtatcttca cgcagtacgg cgcgcggtcc catctcggtg gtcattgcac
    10981 agatatccag acccagttgc tcactccagt aatcaagaca gtaccagttt agcgtatgct
    11041 gtacgtcgtg atattgctgg cgcatatatt ccatcgcttc ctgtggcgta accccgtttt
    11101 tcgcgcccca tgtttcaggc accagctttt gccagaaata gttatcgaag gcgaggtcga
    11161 gcaacgtgcc gtccatatcc agcagaacgg tatctacgtc ctgccaggca atgttgatat
    11221 gcatgaggga aatctccaga gtgaagcaat ttgcgcgaca gggtagcata acctgtcgcg
    11281 caaacgtgtt attcgataag gctttctgaa ggggtgatca gttgcgggtt caggcagctt
    11341 tcataatagg cctgaatctc catcatgcga gtgcgatgac gctggtaacg acgccaggcc
    11401 tgtacgccat tgtaaatggc gctacccagc atcgtcagta gcagcaatgt ggtgctgaga
    11461 tagcgccaca ggccggaacg atccgggatc ggatgaaggc caatatgctg cgtaccattg
    11521 gcgtcagtga agatttttgt gacgatacct tcggcgttaa acggcgtatg catcagcatt
    11581 tgtgccagtt tctggaaagc gttccactgt tcttgcggcg ggtagtcgta aagtgatgcc
    11641 gaaggccagg gctgatcaac aaaatcgctg ccttcgtcgc tgacaatcag gaatccgccg
    11701 ggggccggac tgtttagtga ttgtgccgct cgcgccgttt catgcgtgat aaacggcgcg
    11761 gtggaggttg ccaccaggtt atccagcgat tccgcactca ccgggcgtaa taacacattc
    11821 acgccatcga gcttcccggc gttggcgcgt tttaccagcg cgtcccagtc tttactgttg
    11881 ccgagattga ccagcgcatt tttcagtcgc acacagtcat ctttggcaga acataaatcc
    11941 gctgtcttca gtacaatgtc gccaaaatca tcaagcaata ccatgccgga tttttgaatc
    12001 gctgaacgta atgaggcact gacgcgagat tcatcttccg gtttagggtg cagctggcga
    12061 ttaactgctt cagtcaatgc cgtcgctttg ttgaccagtt cagattctgg taatggtaat
    12121 gagcgggcgt cattccagat gatctgcgag cagtcaaacg gtaaaaaagg tgaattggtt
    12181 ttcgcgctcc aggttccgga agtgcgaata ttacacattc ccgtaccgct aatacgcaat
    12241 gtatcgccta cccgcacgcc agcgtcagcc agttgtttta cgctggtggc ttcaatggtc
    12301 tgcgcgcctt tcatccatga gagagtgaat ttcagcggca tatccagcgg gatccagaat
    12361 aacagcataa acagcaccag cagcgagccc gcagcgataa tcgtactgcg cagccagtgc
    12421 tgtaacggaa agttttttac ttcatcatgc agcgaaagat atcgcccctg acgcactacg
    12481 tggcagtcga gatagatatc aatatcggtt tgttgatcga gatcctgagc aatgtatggc
    12541 tgccagtgtg cgggatagac caggtcgata ataccgagtg aaatattgtt gatctgttcc
    12601 tgatcgtttt cgccaaacaa tccccaacga cggggtgtac cgcgcagaca atgaatttcc
    12661 cgcagggagg attttgccgg gggcgcgaat agcccccaca gacctgcgcc cagcagcagt
    12721 aatgcgccgc ctgccagcca cggaacaaat acatccgggg taatcaggca gaaaaagaac
    12781 atcaggaagg aggcgacgat cagcaacgct tcacgcagcc cgcgcggacg actcagcgca
    12841 tattcttcat gcgtttcttt gcgaatattg agtagctcga tttgctcact ctcttccccg
    12901 cgaatcgacg cctgcgtgga cggaacaggt tgcaaggcat agccgcgcgt ttcctgcatg
    12961 tactcctgta gcgtatgacc gttgagtgaa ataaccaacg gcagcgaatc ggtatgaatc
    13021 agttcaacgg tattctcatc gttgatgtac tgttcccaga aggggggcag gtggacttct
    13081 accgaatcga ggtagtaacg ccatttattc ggatcgtcgg tagagatgcc gtaacgtgtg
    13141 atagcgtgtg tcagcatcat gacgttgttg ctttcggcat tcagcgccag agagatcggt
    13201 gccgcgctag ctcccgttgg gccaggcacc tgtaatacct gcgtcaggct ctcaagataa
    13261 ttttcaacgg cgctacgttc ttcaggtgtg agtttacgcg tttgcgcatc cgcgaaggcg
    13321 ttggtccagg gcagctgact ccgtctggat cgcactttta tcagccatcc cgcaagtagt
    13381 gagcaggcca gcaaagcagc taaaaaaatc acaatggtgc tcatgctttc cccatcttac
    13441 ttagtctgtc aggcgtggtc agtcgcacca gacacaatac atgattctgc ggttggctat
    13501 cggcaagcgt ggagtttaac ttgagcatca ttaagcacat cacagtaacc gctgatcata
    13561 gcaaaccctc tgaaggcgga atatcagcaa attcctagag ttatttcaac ctattgactt
    13621 ttaatttgat ttgaggacga ttatttatta agttacataa atgtaaataa catgcaattc
    13681 acattgccga aagcgtgtcc gatatcgcac aataaccgct gatttcacag catagatccc
    13741 acgatgagca aatcattaca aaaacccacc attctgaatg ttgaaactgt agcccgttcc
    13801 cgactgttta ccgtcgagag cgtggatctg gagttcagca atggcgtgcg gcgtgtttat
    13861 gaacgaatgc gtccaaccaa ccgggaagca gtgatgattg tgccgattgt ggacgatcac
    13921 ctgatcctga tccgcgaata cgcagtggga actgaatcct acgaattagg tttttcgaaa
    13981 ggattaattg atccgggtga aagcgtctac gaagccgcta accgcgagct aaaagaagag
    14041 gttggatttg gtgcgaacga tctgactttt ttgaagaagc tcagcatggc accgtcttac
    14101 ttttccagca aaatgaatat cgtggtcgcg caagatctct acccggaatc gctggaaggc
    14161 gatgagccag agccgctacc acaggtgcgc tggccgctgg cgcatatgat ggatttgctg
    14221 gaagaccctg acttcaacga agcgcgtaat gtcagcgcgc tgttcctcgt gcgcgaatgg
    14281 ttgaaagggc aggggcgagt gtaaatgcgt gaagtttgcc tgatgcacaa cgcttatcag
    14341 gcttatgggg ttcctgcaat atattgattt ttcacgattt tgttagccgg gtaacgcggt
    14401 cacccgcatc cagcataaac aaatgcactt tgtcagccaa ctgaaaaggc gccgaagcgc
    14461 ctttttaatc agaacaattc ctgtgcctca ccattatcga taatggtcgt tcccacctcg
    14521 tgcaccgcct gttgtgtcgg ctgcgtacct tcgatgaaat actcttcgcg gctgttgcca
    14581 ccattagcta actgcccggt gctgcgatcg atattcaccg tcacaatacc cggtggcggc
    14641 gtcagcggct gctccggcac accttcaaga acggctttca tataagcgtc ccatgcaggc
    14701 tgggcactct tggctccgcc ttcgtaacct gagatctgat ctttaatcgc tccggaagcc
    14761 gttgtatgac cgagattacg acggtgatca tcaaagccaa tccagaccga ggtcacaacg
    14821 cccggaccgt aacccgagaa ccacgcatct ttcgaactgt tagtggtacc ggttttcccg
    14881 ccgatatcgc gacgctgcaa atcacgacct gcacgccagc cagtaccctg ccagcctggc
    14941 tcaccaaaga tattggtgtt caaagcactc ttaatcagga atgccagcgg agtgttgatg
    15001 acgtgcggtg cgtactcctg cgcgccagtc ttcgccacta acgcctgatt tgcctgctcc
    15061 agctgcggca ttggtacaga aacattctgc tgctcgcggg agatagcgac atcttcaaca
    15121 tcgttatttt ccagcacgtt cgatttctgc gtatcaccgt aaatcaccgg aatatcgcat
    15181 tccgggcagg ctactttcgg tttcgcttcg aaaatcacgc cgccctgatc gttttcaatt
    15241 ttgctgataa accacgggtc caccaggaag ccgccgttcg ccatgaccgc gtagccgcgc
    15301 gccacctgca ttggggtgaa ggacgctgaa cccagcgcca gcgattcggt gtggacaatg
    15361 ttttgcgccg ggaagccgaa gcgttgcaga tattctgcag cgtagtcgac gcccatagcc
    15421 cgcattgcac gtaccatcac cacgtttttc gactgaccca gcccctgacg taagcgaatt
    15481 ggaccagcat attgtggcgg cgagtttttc ggctgccagt cagaaccggc acctgcgtcc
    15541 cagcgagaaa ttggcacatc gttcaacata cttgccagcg tcagaccttt atccatcgcc
    15601 gcggtgtaga ggaacggttt gatgttggaa cccacctgac gcagtgcctg ggtggcgcgg
    15661 ttaaacttgc tctgattgaa atcaaagcca ccgaccagcg ccataacggc accgttttgc
    15721 ggattgatcg acaccagcgc cgagttcact tccggcactt gtgccagcca ccatgcatcg
    15781 ccaacctgac gaacccagat ttgctgaccc gtttgcagaa catcggtcac tttacgcggc
    15841 gtcggtccct gctgagtatc cgaacggtaa ggacgcgccc agcgaacgcc ttccatactc
    15901 aatgcgacgg tcgacccgtc cgccagcatc gccgtcgctc cctgaggatt ggcgctggtg
    15961 actgcggcag gcagcagcgg accataggtc ggcagcgcct tcagcgtatc ggtaatcttg
    16021 ttgttatccc acgccgactc gcccactttc cacagcacat ttgccgggcc gcgatagccg
    16081 tggcgcatgt cgtagtccag cacgttatta cgtaccgcct gctgtgcggc ctgctgcact
    16141 ttgcgggtga tggtggtgta aatgcgataa ccgtcttcat aggcactttc gccataacgg
    16201 ttatacatct cctggcgcac catttcgctc aggtacggcg cagagaaagc aatctccggc
    16261 gcgtgatagt tagcgttaat cgcctcagtg cgtgtctgat cgaactgttg ttgggtgata
    16321 tacccttcat ccagcatccg cgacagtacg acgttacgcc gcgcgacggc acgatccatt
    16381 gagtagagcg gattaaaggt ggaaggcgct ttcggcagcc cggctatcac agccatttcg
    16441 ttcagcgtca gttggtcgac cgttttaccg aaatagactt gtgccgcagc accgacacca
    16501 taggcgcggt aaccgaggta aatcttattc agataaagct cgaggatctc gtctttcgtc
    16561 agcaactgtt caatgcgaat cgcgaggaag acttccttaa tcttacgcat cagcgtgcgt
    16621 tctggactga ggaagaagtt tctcgccagc tgctgggtaa tggtactcgc cccttgtgac
    16681 gcgtggccgg agaacagcgc cacacttgcc gcacggaaga tccccaccgg gtcaacgccg
    16741 tgatgctcgt agaagcggct gtcttccgtc gcgataaagg ctttcaccat ctccggcggg
    16801 atttgatcca acgtaaccgg aatacgacgt ttctcaccgt attgagcaat cagctcgcca
    16861 tcggcgctgt aaatctgcat cggaatttgc aggcgaacat cttttaatgt cgccacatcc
    16921 ggcagttgtg gctcgatgta gcggtatagg ccataaatcg agcctgctcc cagcagaatg
    16981 caacagactg caaggatcaa aaaatacttt acgaacttca ctggaaattt cccatttagt
    17041 ttcatttggg cagtttataa acaaacgcgc ggtagtataa aggcaagcca gacgcattga
    17101 tatacccgtc agagtgacgg gtgataagga gatcatcaca atggcattta agatctggca
    17161 aattggtttg catttacaac agcaagaagc ggtagcggtt gcgatcgtgc ggggcgcaaa
    17221 agaatgcttt ttgcaacgct ggtggcggtt gccgctggag aacgacatta tcaaagatgg
    17281 gcggattgtt gatgcgcagc agctggctaa aacgttgtta ccttggagtc gcgaactgcc
    17341 gcagcgtcat cacattatgt tggcgtttcc cgccagtcgc acattacagc ggtcatttcc
    17401 gcgcccgtcg atgtcccttg gtgagcggga gcaaacggcc tggctgtcag ggacgatggc
    17461 ccgcgagctg gatatggatc cggactccct gcgcttcgat tacagcgaag actcactcag
    17521 tcccgcctat aacgtgactg ccgcgcaaag caaagagctg gcaacgctgc ttactctggc
    17581 agaaaggttg cgtgttcatg ttagtgcgat caccccggat gccagtgcat tacagcgatt
    17641 cctgcctttt ttaccttctc atcagcaatg tctggcctgg cgtgataacg aacagtggct
    17701 gtgggcgacg cgctatagct gggggcgcaa actggcggtg gggatgacta gcgcgaagga
    17761 gctggcggca gcgttattcg ttgatcccga cagcgtcgcg atatgtggcg aaggcggatt
    17821 tgacccctgg gaggccgttt ctgttcgtca gccgctgcta ccgccgccta gtggagactt
    17881 tgccatcgcg ctggggctgg cgcttgggaa ggcgtactga tgaacccgcc aattaatttt
    17941 ttgccctggc gacagcaacg ccggaccgct tttctgcgtt tctggttgct gatgttcgtt
    18001 gcgcctctgc tgctggccgt cgggataacg ctaatactgc gtctgacagg cagcgccgaa
    18061 gcgcgcatag acgcagtttt gcttcaggcg gaacaacaac tcgcccgcag cttacagata
    18121 acgaagccac gtttgctgga gcaacagcag ttacgcaaac agcgttcgca acggcagcgc
    18181 cagcgacaat ttacccgcga ctggcaatct gcgctggaag cactggcggc gcttttaccc
    18241 gagcacgcct ggctgacaac gataagctgg cagcagggaa cgctggagat caaggggctg
    18301 acaacaagca ttaccgcgtt aaacgcacta gaaacgtcac tccgccagga tgcgtctttt
    18361 catctcaatc agcggggagc cacgcagcag gatgcgcagg gacgctggca atttgagtat
    18421 cagttaacaa ggaaggttag cgatgaacat gttctttgac tggtggttcg ccacatcacc
    18481 ccgcctccgc cagttttgct gggcattctg gttgctgatg ttagttacgc tcatttttct
    18541 gtcatcgaca caccatgaag agcgcgacgc attaattcga ttacgggcaa gtcatcacca
    18601 gcagtgggcc acactgtatc gcctggttga caccactccc ttcagcgaag aaaaaacgct
    18661 gcccttttcg ccactggatt ttcagttgcc cggcgcgcaa ctggtttcct ggcatccatc
    18721 cgcgcaggga ggtgagttgg cgttgaaaac gctgtgggaa gcagtgccgt cggcattcac
    18781 acggctggca gagcgcaacg tcagcgtgag ccgtttttcg ttaagcgtgg aaggtgatga
    18841 tcttttgttc acgctacaac tggagacgcc gcatgagggt taaacgctgg ttgttggcag
    18901 gtattgcatt gtgcctttta accggtatgc gtgacccttt taaaccgccg gaagatctat
    18961 gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga ggcgagcgca
    19021 tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa aacgatgtgc
    19081 tggaaaacgg ctggacaatt ttacagctga cgccagacgt actaacgctg ggtaccggga
    19141 caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat gaagcaatgg
    19201 atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc gcaaaaagtg
    19261 acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc tgaacaggag
    19321 aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca tctaacagat
    19381 gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat aacgcggcag
    19441 gaaggcaaca ttctctcagt gcattccatt gcctggcaga ataacaatat cgcccgccag
    19501 gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag tataaccctg
    19561 caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag tgccaaaggg
    19621 agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa aacggcgtta
    19681 agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt tgagctgtcg
    19741 gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa atggacgctg
    19801 gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag cgacctctcc
    19861 gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg acgcttgctg
    19921 gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc cagtccgcgt
    19981 ctgctggcct cacatcttca gcctgccagc attaaacagg ggagcgaaat tccatatcag
    20041 gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt cctggggata
    20101 gaggtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca catcagccag
    20161 aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat tgataagcag
    20221 gagatcgaaa cgcaggtcga ggtcaaaagc ggagaaacgt tagcgctggg cggcattttt
    20281 acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat tccctggttc
    20341 gggcaattat ttcgtcatga cggaaaagaa tatgaacgac gcgagttagt ggtgtttatc
    20401 acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt tttatgctga
    20461 acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac cgatttgaga
    20521 gttggcgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg cggcattttg
    20581 ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg tagcaaggca
    20641 gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt gccaaacccg
    20701 ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg tttcagttca
    20761 tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc ttagtagtac
    20821 cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg ccggaaaaag
    20881 cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt ccgatcaaga
    20941 gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag gcgaagaagg
    21001 cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg gtattgtgct
    21061 ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt ccgctcgtgg
    21121 cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc agcgtgataa
    21181 aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag cgttggccaa
    21241 tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg atgatcaaag
    21301 cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat tctggcttta
    21361 tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga gaggattgtc
    21421 gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt taatgaacca
    21481 gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccaa cgaaaccctg
    21541 gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt taacgtcgat
    21601 agcgttatcc tccctgacgg cgagcagtat aaaagcctgg ctgtactcga taccgtcttt
    21661 acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct tggcggcggc
    21721 gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt tcgtttcatt
    21781 caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa aactgcggtc
    21841 aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc agtggtggtg
    21901 gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct ggcagaagtc
    21961 atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga gaatctggat
    22021 gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg ttgtgaactg
    22081 aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt actgaatctg
    22141 ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg gttacatggt
    22201 gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct cgggcagttt
    22261 agttctgccg aaacgcagcg tattataacc ctgctcaagc gggctgggtt accggtcaat
    22321 gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga caagaaagtc
    22381 cttgcgggag agatacgctt aattcttccg ttggcaattg gtaagagtga agttcgcagc
    22441 ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc gtaacaacaa
    22501 gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca acgtggtaag
    22561 cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg agctgaaacc
    22621 cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt ctgagcgtac
    22681 tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca ctgacgatcg
    22741 ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag aagaaattga
    22801 cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc gtaagcgcaa
    22861 aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg gcattctggt
    22921 tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga cctcttccag
    22981 cgatcaaacc gcgtctggcg ataagagtat tgatcttgca ggcaatgcga ccgatcaggc
    23041 gaatggggtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc aggatgtttc
    23101 tctgccaccg atctcttcta cgccgactca agggcaaacc ccggcggcaa cggatggtca
    23161 acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg actcagccac aaaatcagca
    23221 acagttgaac aatgtggcgg tcaattccac attgccgacc gaacccgcga ctgtcgcgcc
    23281 tgttcgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaaccgctg aacgtccgtc
    23341 cactacgcgc ccagctcgtc agcaggcggt gattgaaccg aaaaaaccgc aagcaaccgt
    23401 gaaaacggag ccgaagccgg tagcacagac gccgaagcgt actgaaccag ctgcccctgt
    23461 ggcgagcacg aaggcaccgg ctgcgacttc tacgccagca ccaaaagaga cggcgactac
    23521 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
    23581 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
    23641 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
    23701 ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
    23761 gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgttcaggc
    23821 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
    23881 atctcccgca atggtttatc gttgcgggag ctgcctgaag cgctggatgc tgtcggagct
    23941 ttctccacag ccggagaagg tgtaattagt tagtcagcat gaagaaaaat cgcgcttttt
    24001 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
    24061 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
    24121 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
    24181 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
    24241 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
    24301 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
    24361 gcggtgagtt taacgtgccg tttggccgct acaaaaaacc ctatttcccg gaagcagagt
    24421 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    24481 gcatggcgcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
    24541 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    24601 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
    24661 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    24721 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    24781 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    24841 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    24901 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    24961 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactatg gcattaccgc
    25021 ccctatcgat gtacatctga tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc
    25081 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    25141 gcaactgatt aaagaaaatg gctgtaaagc gggtctggta tttaacccgg cgacacctct
    25201 gagctatctg gattacgtga tggataagct ggatgtgatc ctgctgatgt ctgttaaccc
    25261 aggttttggc ggtcagtcgt tcattccaca aacactggat aaactgcgcg aagtacgtcg
    25321 tcgtatcgac gagtctggtt ttgacattcg actggaagtg gacggtggcg tgaaggtgaa
    25381 caacattggc gaaatcgctg cggcgggcgc ggatatgttc gtcgccggtt cggcaatctt
    25441 cgaccagcca gactacaaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    25501 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tctcgacggt acgctggttg
    25561 acagtgctcc cggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttacccg
    25621 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatgtt ctgatggagc
    25681 gtgcattggc ctgggcgcgt caggaacgtg cgactctgcg taaaacaatg ggtaaaccgc
    25741 ccgttgatga cgacattccg gcagaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    25801 gctactatag cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    25861 tgggcgcgtt gcaggctaaa ggcctgccgc taggcctggt caccaacaaa ccgacgccgt
    25921 tcgtcgcgcc actgctcgaa gccttagata ttgccaaata ctttagcgtg gttatcggcg
    25981 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgagcgga
    26041 tgggaattgc cccacaacag atgctgtttg tcggcgactc acgcaatgat attcaggcgg
    26101 caaaagcggc aggttgccca tcagttggct taacctacgg atataactac ggcgaggcta
    26161 tcgatctcag ccagcctgat gtaatttatc agtctataaa tgaccttctg cccgcattag
    26221 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    26281 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    26341 aacatgcagg atgactacca ttgcatttac tgtatcgttg accaacacgc gatcaccgtg
    26401 cgccaggatg cacagaagct gcgtaaagcg acgctggata cgctggcctt gtatctggct
    26461 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtacc agaacatgcg
    26521 cagttaggct gggcactgaa ctgctatacc tacttcggcg aactgagccg catgacccag
    26581 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tgactatccg
    26641 gtgctgatgg ctgcggacat cctgctgtat caaactaatc tggtaccggt gggtgaagac
    26701 cagaaacagc acctggaact gagtcgcgat atcgcccagc gtttcaacgc gctgtatggc
    26761 gagatcttta aggtgccgga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    26821 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    26881 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    26941 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    27001 ctttctgcgg taacgggcca gagcatccca gaactggaaa aacagttcga aggcaagatg
    27061 tatggccatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    27121 gaacgctatc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    27181 gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag cggtgtacga agcgattggt
    27241 tttgtggcga agccgtaata tcaaaacgct cggacggtcc cctcgcccct ttggggagag
    27301 ggttagggtg aggggagcag gcaagcgcca gtgggaatta ccctttgctc gcattgtcga
    27361 ataaatcact tcgtcatcac tctgaaaccg gggaaacccg gtttttttat cctcatttgc
    27421 aataatccga aaaaatgtga agcgcctcgc cgtttccaca tctcacagtt gccacttatc
    27481 ttcattttaa tgaagatata aacattcatt ttattgaaaa tatattatgc gtcgaacgtt
    27541 tatcaaaaaa gaaggcgtcg tcatcacgac gctggcccgt tatttgttgg gtgaaaaatg
    27601 cggtaatcga ctgaaaacca tagatgagct ggcaactgaa tgccgttcat cggttggcct
    27661 gacgcaggcc gcgttgaaaa cgttggaatc aagtggagcg atacggattg aacgccgtgg
    27721 gcgcaatggc agttatctgg tcgaaatgga taacaaagcg ctgctgagtc atgtggatat
    27781 caataacgtt gtgtgtgcca tgcctttgcc ctatacccgc ttgtacgaag gtttagcaag
    27841 cgggctgaaa gcccagtttg atggcattcc tttttattat gcgcatatgc gtggcgcgga
    27901 tattcgtgtg gagtgtttgc ttaacggtgt ctatgacatg gcagtagttt cccgcctggc
    27961 ggcggaaagt tatctcagcc aaaacaattt gtgcatcgtc ctcgagcttg gcccgcatac
    28021 gtatgttggt gaacatcaac tgatatgccg caaaggtgag tctggaaatg ttaagcgagt
    28081 aggtctcgat agtcgttcgg cggatcagaa aatcatgacc gatgtctttt ttggcgatag
    28141 tgatgtggaa cgtgtcgatc tctcttatca cgagagttta caacgcattg ttaaaggcga
    28201 tgtcgatgcg gttatctgga acgtggtggc ggaaaacgaa ctgaccatgc tgggattaga
    28261 ggcgacgccg ctcacagacg atccgcgatt tttacaggcc accgaagccg tggtcctgac
    28321 gcgagtcgat gattacccaa tgcaacaact gctgcgtgcc gttgtagata aacacgccct
    28381 gctcgcccat caacaacggg tagtgagtgg ggaacaggaa ccgagttatt aaacgaaagg
    28441 catctgatat ggaaaccaga ctcaacctgc tttgcgaggc aggcgttatt gataaggaca
    28501 tctgcaaagg catgatgcag gtcgtcaacg tactggaaac agagtgccat ctgccggtgc
    28561 gcagtgagca aggaacgatg gcgatgacac atatggcgag tgcactgatg cgcagtcgcc
    28621 gtggtgaaga aatagagccg ctggataacg aattgctggc agagctggcg caatccagcc
    28681 actggcaagc cgttgtgcaa ttgcatcagg tgttgttgaa ggaattcgca ctggaagtta
    28741 atccgtgtga agaaggctat ttgctggcca acctttatgg attatggatg gctgctaacg
    28801 aagaggtttg aagctcccga caaatccacg tcgcacaacc taaatattca aaaaaatgaa
    28861 tatttaattc aaggaacaga aacgaggcaa caagatgttt gtagaagcat tgaaacgcca
    28921 gaacccggcg ctgatttccg ccgcactaag tttgtggcag cagggcaaga tcgccccgga
    28981 cagctgggtg atcgacgtgg atcaggtact ggaaaacggt aagcgactga ttgagacggc
    29041 gcggctttac ggcattgaac tgtatctgat gaccaagcag tttggccgta acccatggct
    29101 ggcggaaaaa ttgctggcat taggttacag cggcattgtg gcggtggatt acaaagaggc
    29161 gcgagtcatg cgccgcgctg gtttgtcggt ggcgcatcag gggcatctgg tacaaatccc
    29221 ttgtcatcag gttgctgacg ccgttgaaca gggcaccgac gtcatcaccg tgtttactct
    29281 cgacaaagcg cgggaagttt ctgcggcagc ggtgaaggcc ggacgtgtgc agtttgtgct
    29341 gctcaaagtt tatagcgacg atgattttct ttatccgggg caggagagcg gttttgtaca
    29401 gcattcgctg cacgaggtgg ttgctgaaat taagaaactg ccgggtctgc atttagccgg
    29461 acttacccat ttcccttgcc tgctttggga tgaggctgcc ggaaaagttt tgccgacacc
    29521 gaatcttcac acgctgatac aggcacggga tcaactggcg aaatctggta ttgcacttga
    29581 gcaactgaac gcgccttcag cgaccagctg cacttcgctg ccattactgg cgcaatacgg
    29641 tgtgactcat gccgaacccg gtcatgcgct gacgggcact attccggcaa accagcaggg
    29701 cgatcagtct gaacgtatcg cgatgctctg gttgagtgaa atctcccatc atttccgtgg
    29761 cgacagctac tgctacggcg gcggttacta ccgtcgtggt catgcgcaac atgcgttggt
    29821 gtttacgcca gaaaatcaaa agattactga aaccaatctc aaaactgtgg atgacagcag
    29881 tatcgactac accctgtcgc tggcaggcga gtttccggta agcagtgcag tggtgctctg
    29941 tttccgcacg cagatttttg tcacccgtag cgatgtggtg ctggtgtccg gtattcaccg
    30001 tggcgaaccg aaaatcgttg gtcgttatga cagtcttgga aactctctgg gggcgtaatg
    30061 gcgcgatttg tggtgttagt gattgatagc tttggcgtag gggcaatgaa agatgtcacg
    30121 ctggtgcgtc cgcaagatgc gggagcgaat acatgtggtc acatcctgag ccagttgccg
    30181 catttgcagc taccaacgct ggagaagctg gggctaatca acgcattggg ttatgcgcca
    30241 ggcgatatgc agccgtcaga ttccgcaacc tggggcgtgg cagagctgca acatgaaggt
    30301 ggcgatacct ttatggggca tcaggaaatt ttaggcacgc gcccgttacc gccgctgcgg
    30361 atgccttttt gcgatgtgat tgaccgtgtt gagcaggcat tagtatccgc cggttggcag
    30421 gtggagcgcc gtggcgatga actgcaattt ctgtgggtca atcaggcggt tgcgattggc
    30481 gataatctcg aggcggattt aggccaagtc tataacatta ccgccaatct ctctgtgatc
    30541 tcttttgacg acgcaatcaa aattggtcgt atcgtgcgtg agcaggtaca ggtcggtcgg
    30601 gtcattacat ttggtggcct gttaaccgac agtcaacgca ttctcgatgc cgcagaaagc
    30661 aaagaagggc gctttattgg tatcaatgcg ccgcgttctg gcgcttatga caacggcttc
    30721 caggtcgtgc atatgggcta cggtgtcgat aaaaaagtgc aggtgccgca aaaactgtat
    30781 gaggcgggcg tgccaaccgt gctggtgggt aaggtggcag atatcgtcag caatccttat
    30841 ggcgtgagct ggcaaaacct ggtggatagc cagcggatta tggatatcac cctcgacgaa
    30901 tttaacaccc atccgacggc gtttatttgc accaatatcc aggaaaccga cctcgctggt
    30961 catgcagaag acgtcgcacg ttatgccgaa cgtttgcagg tcgttgaccg taaccttgcc
    31021 cggcttgttg atgcgatgca gccagatgat tgcctggtcg tgatggcgga tcacggcaac
    31081 gatccgacca ttggtcacag ccaccatacc cgcgaagtgg tgccgctgct ggtttatcag
    31141 caaggcctgg ctgctacgca gctcggtgtg cgcaccacgc tttctgatgt gggagctacc
    31201 gtgtgtgaat ttttccgtgc gccaccgcca caaaatggtc gctcttttct ttcctccctc
    31261 cggtttgcag gagacaccct atgagttttg atccgacggg ttacaccctg gcccatgagc
    31321 atctgcatat tgatctctcc ggctttaaaa acaacgtgga ctgccgcctt gatcagtatg
    31381 cgttcatttg ccaggagatg aacgacctga tgacccgggg tgtacgtaat gtgattgaga
    31441 tgaccaaccg ttacatgggg cgcaatgcgc aatttatgct cggtgtaatg cgcgaaaccg
    31501 ggatcaacgt ggtggcctgt actggttatt accaggatgc gtttttcccg gaacatgtgg
    31561 tgacccgcag cgtgcaggaa ctggcgcagg agatggtcga tgaaattgaa cagggtatcg
    31621 atggcacgga gctgaaagcc gggctcatcg cggagatcgg ctccagcgaa ggaaagatta
    31681 cgccgctgga agagaaggta tttattgccg ctgcgctggc gcataaccag accggacgcc
    31741 cgatctccac gcatacgtcg ttcagcacca tggggctgga acaactggct ctgttgcaag
    31801 cccacggagt tgatctttcg cgcgtcaccg ttggtcactg cgatctgaaa gacaacctcg
    31861 acaacatttt gaagatgatc gatctcggcg cgtacgtgca gttcgacacc attggtaaga
    31921 acagttacta cccggacgaa aagcgcattg cgatgctcca cgcgctacgt gaccgtgggt
    31981 tgctgaaccg cgtcatgctg tcgatggata ttacgcgccg cttccattta aaagccaatg
    32041 gtggttatgg ctatgactat ttattgacca cctttattcc gcaattgcgc cagtcaggat
    32101 tcagtcaggc cgatgtggat gtgatgttac gtgaaactcc ctctcaattt ttccaataag
    32161 gacagactca tgaaaaagat tggcgttgca ggcttacagc gtgagcagat taaaaaaacc
    32221 attgaagcga cggctcctgg cagttttgaa gttttcattc acaacgacat ggaagcggca
    32281 atgaaagtga aatccgggca actggattac tacatcggtg cgtgtaatac cggtgcgggc
    32341 gcggcattgt cgattgccat cgcggtgatt ggctataaca aaagttgcac cattgccaaa
    32401 ccaggcatta aagcgaaaga cgagcatatc gccaaaatga tcgctgaagg aaaagtggcg
    32461 tttggccttt ccgttgagca cgtcgaacac gcgattccga tgctgattaa ccatctgaaa
    32521 taaaaggcac gactatggat ctgtatattc agattatcgt ggtggcgtgc ctgacgggta
    32581 tgacatcgct tctggcgcat cgctcggcgg ctgtttttca tgacggcatc cgcccgatcc
    32641 tgccgcaact gattgaaggc tatatgaacc gtcgcgaggc ggggagtatc gcttttggtc
    32701 tgagcattgg ttttgtggcc tcggtgggga tctcttttac cctgaaaacc gggctgctca
    32761 acgcatggtt actctttctt cctaccgata tcctcggcgt actggcgata aacagcctga
    32821 tggcgtttgg tcttggcgct atctggggcg tgttgatcct tacttgcctg ttgccagtaa
    32881 accagctgct gaccgcgctg ccggtggatg tattaggtag cctcggggaa ttaagctcgc
    32941 cggtagtttc tgcttttgca ctgttcccgc tggtggcgat tttctaccag tttggctgga
    33001 agcaaagtct ggtcgccgcc gtggttgttc tgatgacccg tgtggtagtc gtgcgctatt
    33061 tcccacatct taaccctgaa tccatcgaaa tctttattgg catggtgatg ctgctgggga
    33121 tcgcgataac tcacgacctg cgtcatcgtg atgaaaatga tattgatgcc agcgggcttt
    33181 cggtgtttga agaacgcacg tcgcggatta tcaaaaactt accctatatc gccatcgtgg
    33241 gagcattgat tgccgccgtt gccagcatga agattttcgc cggcagtgaa gtgtcgatct
    33301 tcactctgga gaaagcgtac tccgcaggcg taacgccgga acaatcgcaa acgctgatca
    33361 accaggcggc actggcagaa tttatgcgcg ggctgggttt tgtaccgttg attgccacca
    33421 ccgccttagc aacgggtgtg tatgcagttg cgggctttac ctttgtttat gcggtgggct
    33481 atctctcgcc gaatccgatg gttgcagcgg tattaggcgc agtggttatt tcggcggaag
    33541 tcttgctgct tcgttctatc ggcaaatggc tgggacgcta cccgtcggtg cgtaatgcgt
    33601 cggataacat ccgtaacgcc atgaatatgc tgatggaagt ggcgctgctg gtcggttcga
    33661 ttttcgcagc aattaagatg gcgggttata ccggattctc tatcgcggtt gccatttact
    33721 tcctcaacga atccctgggc cgtccggtac agaaaatggc ggcaccggtc gtggcagtaa
    33781 tgatcaccgg tattctgctg aatgttcttt actggcttgg cctgttcgtt ccggcttaag
    33841 gaaggcacct atgaagacgt ttcctctgca aagcctgacg cttattgagg cgcagcaaaa
    33901 gcagtttgcg ctggtggaca cgatttgccg tcactttccc ggcgcggagt ttctaaccag
    33961 cggtgatttg ggcttaacgc cggggctgaa tcaaccgcgt attacccaac gggtggagca
    34021 ggtgctggct gatgcatttc acgcacaggc tgcggcgctg gtgcagggcg cggggactgg
    34081 cgcgattcgc gccgggctgg cagctttgct caaaccgggg cagcgtcttc tggtgcatga
    34141 cgcgcctgtt tacccgacga cacgggttat tattgagcag atggggctga cgcttattac
    34201 tgttgatttc aatgacctgt cggcactgaa gcaggtcgtc gacgagcaac aaccggatgc
    34261 ggcgctggtg cagcatacgc gccagcagcc gcaggacagc tacgtgctgg cagatgtgct
    34321 ggcaacgttg cgcgcggcag gtgttccagc gttaaccgat gacaactatg cggtgatgaa
    34381 ggtggcgcga atcggctgtg aatgcggcgc gaatgtctcg acattttcct gcttcaagct
    34441 atttgggcca gagggtgttg gtgcagtggt cggcgatgct gatgttatca accgtattcg
    34501 cgccacgctt tactccggcg gtagccagat ccagggcgca caggcgctgg aagtattgcg
    34561 ttgtctggtg tttgcgccag tgatgcacgc ggtgcaggca ggggtatctg aacggttgct
    34621 ggctttgctt aacggtggtg aggtgccgga agtgaaaagc gcggtgattg ctaatgcgca
    34681 gtcgaaggtg ttgattgtcg agtttcatca gccgattgcc gccagagtgc tggaagaggc
    34741 gcaaaagcgc ggtgccttgc cttacccggt gggtgcagag tcgaaatatg aaatcccgcc
    34801 gctcttttat cgcctttccg gaacgtttcg ccaggcgaat ccacaatcag aacattgtgc
    34861 gattcgcatt aacccgaatc gcagcggtga agagacggtg ctgcggattt tgcgtgagag
    34921 tattgccagt atttaatgct ggatagcacc gtcaagataa attccgtact gaacggtccc
    34981 ctcgcccctt tggggagagg gttagggtga ggggaacgta cgggatgtaa aattcaacgc
    35041 gccagcatcg ccggatgcga tgctggcgca tcttatccgg cctacggtta atgtctttta
    35101 ttatcaatag ttaaggtgat gcgattggcg cggcagaaga gttcggagac gtgcaccggt
    35161 ttgtcattct gatcaaaggc gattttgctg atgcgaaaca acggttcgcc cagttcgcat
    35221 ttcagccatt ttgcctgcgg gcgggtggcg gcaaagatat cgatggtctt tttgtcgctg
    35281 accactcgcg tgtcaaaacg ctcctgaaat aactgatagg tggagcttcc ttcgacgtaa
    35341 atctcatcaa agtcaggata acgcgacagc gggatccatg aactatcaat aaacagcggc
    35401 tctttatcga gatacatcac ccggcagaga tggaacactt cgctgtttcc ggggatgttc
    35461 agctttttgc aaaacggcgc ggcgctgacc cgttcctgtt cgatcacttt ctctttcgtc
    35521 gccttgcctt gtgagacgcc aaaatcggta aaaccactga cagtaagcag ggcgttttca
    35581 actttctggc tttgtacaaa ggtgcctttt ccctgccagc ggatcagtac gccgtccgcc
    35641 actaagtcgc tgatggcttt gcgaatggta atgcggctga cgttatattg tgtacaaagc
    35701 tcgttttcgg tagggatctg ttgcccggcc tggtaaaccc cctgcgcgat atcatccagc
    35761 agtcgctggc ggacggtagc gtagaggagt tgatgagagt agcggtccgt agctgacata
    35821 atcgaccctg aaataaagaa tgaattactc ggattatagg gtcgttttcc ggcaagtaac
    35881 agcacgttgg cgtgaagatg ctcacgccaa cgttatacct accaggcacc gtggtactga
    35941 atggttttcg ccgcgcacgc cgttccctgc gccatcgcct gcggtaatgt catccccgca
    36001 gaccagccgc aaaggaatcc ggcaatgaac gaatctccgg cacccatggt gtcgataacc
    36061 gtcaccggtt caggagcctg acgccagaac tgcgcgccat cccaggcaat gctgccgttt
    36121 tcacccagcg tgacaatcac tgttcctgcg ccacgggcaa caatcgcttt catcttcaga
    36181 cgcagcgttt cgtcttcttg cggtgcggag gcaaaggcaa aatcgagatg cggcaccagt
    36241 gtctgccaga gcgggctgtc ccacttgtcg gagaagtcga aagcggtaag tttgcccgca
    36301 gcgtgcagct gtgggaatgc gtcttccgca tgtccccaga ttgccgcgtg cacaatgtca
    36361 tactgcgcca gccaggcgta atcctcttca ctcagggcaa agtcggccat cacgccttcg
    36421 gtgtagtcgc caaaaacgcg atcattgtcg tgcagttcca cctgagtttg tgcggtaacg
    36481 ccgtgtttcg tatggacatg gctgatatcg acgcccatgc gggcgagatc ctgcttcagc
    36541 tttgtgccgt agtcatcgtc acccacccag gtaatgcatc ccggctgtat gccgtagcga
    36601 gtgcagtaca ccgccacatt gaccgcatta ccgccagaaa acgctttatt cagttgcggg
    36661 tagatatcga cgcagttatc gccgattgtc gccagggttt tcatctctca tcctccggca
    36721 gcagcgcgcg aaagcgttcc agcgcctggc gggcatagag tctgggctcg ttcatataca
    36781 tcgtcaccag ctccaccgta cagtaaccct catagccccg ctcaataata tcgcgcatca
    36841 gttcccgcag cggcattttg ccttctccag gaatgtaatg cgtgtcgctg gccccgtcgc
    36901 tgtcgacaat atgcagatga cgtaatttat cgcccagttt gtcgaaataa ctcatcaccg
    36961 gttccgcctg gacatacggc gcgcaaatgt cgaccatgct gaacaggcgc ggcgaaggca
    37021 ccagcgccag cgcatgaagc acatcattag cattacacac gatgttcgat tcatacggcg
    37081 ttaacggttc gaggattaga tccatgccga tgttttccgc gtactcatac agttcgctta
    37141 ggttctctgc cagccgtccc cagataacat taggtggcgt gagatagccc gcgtgggccg
    37201 cggaaatcag cgtataaccc gcgttcatct cttttgccat atccatcgcc agcttgatca
    37261 tgtcgaggct ttcgcgacgc atatgttcat cgcccagcat catgttatac ggatagccat
    37321 tggtttctgg cgtatagccg ataatcggca tctgatacgt ctgcgccagc gccttgattt
    37381 gtttgatgcc gcccgctttt aagtccggcg cgaacgcgtg cgggcgaccg ccccaaattt
    37441 cgatgccgtc gtaacccagc tcgcttgcat cacgaaatgc gtgttcaata ggcagccgct
    37501 ggtggccgca ggtaaacata cctgttttca tcgggtaact ccttgcgtaa atccggcccg
    37561 ttgccgggcc gggcgaggga ttaatattcc accagaccac cgtaatagcg gcgttcatcc
    37621 gggttgtgat ctttgtaaat agacaggtag tagcagagcc actccattgg cacgaacatc
    37681 aggaacggtg ccagccacgg gtgcagccct tgcgaaattt cggcgtaatc gatgacgatc
    37741 acgttgtcag tacgctgttt aacaaagtta atggcgcgtt cggtggtgtg gcgactttca
    37801 tcattgccga gcaggaacag gaacggaacg cccggttcga caatctccag cgggccatgg
    37861 cggaactctc cgctctcaat cacgcagccg tgcgtccagg taaattccat cagcgttaca
    37921 atgccttctt tgtaacccag cggacgcagc ggacccgcag caacggtata aatcatcggc
    37981 cactggctgg ccagttcacc aagctggcgg cctttttctt cccaggtgcg taccagatga
    38041 ccgagcgcat tcggcaactg cttgagatcg tttttgatct tgccgatttc cgcgttcggc
    38101 gcgaggcggg tgatcatctc cagcaccacg ctgtagcaga gcagcaggtg aatttcccag
    38161 atacagtcgg cctgataatc aatgctaaat tccgccgccg aggtaatcgg gctgtccgcg
    38221 cgtttggtga acgccgcagt gagtgcgccg caggcccggc ccagctccag cgctttgatt
    38281 acctcttccg ttttaccgta gtcagaaacg ccaatcaccg cgcaacgatc gtcgaggcga
    38341 tacggggtgt tatcgcagaa ctcccagccg gaaatggcgt agacctgaag atcggaaaag
    38401 cgatccgcca gatgtttcgc cgcttgcgcc gcgttgagtg gcgagccgca ggcaacgaaa
    38461 taaatacgat caatgtcgcg cttcaccatc tcttccacga tggcgtgcac cagcggaacg
    38521 tcatggctga gaactttttc cacttcctga accatatttt cggtgaccag aaagtccacg
    38581 gtgcttttat caatatccaa cattttattt tctccagata acattacgac aaagcgttga
    38641 gctggcggct acgcttcgcc cagaaggcgt aagcaggcag accagtagca ataacgatga
    38701 cggcgcagat aaggccggga atcggtgccc agacaaaggt tgaggcgacc agaatgaggc
    38761 ttgacgcaat ggcgagggtg gtcatcagcc cgaaagcagg agtacgccac agcggtttgt
    38821 aatcgtcgcg tttacgacac cagatgatgg agccgaaggt gagggtattt ttgaaacaca
    38881 tcaccagggt gaaataaccc agcaggctgg tgagatcgga aacgaagatg aagaagagcc
    38941 ccagcgcccc ttgcaggatg atggagacat ccggcgtgtt gtatttcgga tgcacatggc
    39001 cgaagcattt aaagaacagg ttgtctttcg ccatcgcgta ttccagccgc ggctggtaca
    39061 tcacgcagct ggaaagcgaa ccaagaatga cgatcatcgc cgtgatggca acaaagatcc
    39121 ctgcggtgct gccgagtgcg gggatccagg tcagggcgtc ggaaatcggc gtttcagaat
    39181 tggcgagttt gtcgaagggc atcaggtcgg aaatcaccag tgccagcagg gtgtagagca
    39241 ccagaaccag cagacaggaa ccaatcagcg ctcgtggcat ggtttttccg gggtttttaa
    39301 tttcgccggt catataacag atagaggcca tgccggtata cgaccaactg gtggcagaga
    39361 tccccgccag cagcgccata aagctgcccg ttgcgccaat cgcagtggtg gtaggggcgg
    39421 caaaattctc cgctttaaac cagaagatcc caaggccaat gacgatagtg aacgggataa
    39481 ttttggcgat ggtaattagc gtctgaaacg ctgcgccgcc ttcaacggag cgcaggtgta
    39541 gcaacataaa ggcgataatt aatccggcgg cgataaattt accgggcaac ggatcgatag
    39601 gcgttaaaaa gccaagattg ctgacaatcg ccagcgccat aatcgacaat gacggcgcat
    39661 cgttggccca gaagctggcc cagccggaga ggaaagccag cggtcggctt ccggcatttt
    39721 tcagataaac ataatctgcg ccattttccg gataagcggt ggatagttcc gcatagacgc
    39781 acatttgcgg gatcacaatt aacccgccaa tgacaaacgt gagcaccgta agccacggcg
    39841 tgcccgctgc ttttgccact tcacccacag atacaaaaat accggagccg acggttgtcc
    39901 cgacggcgat tgcaagaacg gcccaaaatc cgagcttgcg ttggagttcc tggcttccca
    39961 tagcgatacc tttttcttgt ccaacacgtt gtaggggaga gtttgcagtc atttaaaagg
    40021 taacatgaca atgcatgatg aatataataa catatgatgt tatgtaatgc ggaggaagat
    40081 cacaaaattt cgcacaggat cgcgctgtgg ctaatgaatg tagttatcca attgaaatta
    40141 aagtgaaaat atttttacgg gtggggagca agaaggatat agaaacaaat acgccctcgg
    40201 aaaatccaga gggcgtcggg caattaaacc ggtgttagcc gatttctgtc agagacttac
    40261 tgtgcagtag gaccgcaacc gccaatgatt ttagaaatag aaattgccgg gtgcagcagg
    40321 tagtcgtaag agcagttctt atcacggttt tcaatatgac cagtacatgc agttaacgta
    40381 gccagtactg cacctaccag tgcaacttta ataaatttgt tcatt
//
LOCUS       DADLTD010000010        39778 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLTD010000010 DADLTD010000000
VERSION     DADLTD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.010"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(160..900)
                     /locus_tag="JN701_000405"
     CDS             complement(160..900)
                     /locus_tag="JN701_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY7362949.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(993..1628)
                     /gene="argO"
                     /locus_tag="JN701_000406"
     CDS             complement(993..1628)
                     /gene="argO"
                     /locus_tag="JN701_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY7362950.1"
                     /translation="MFSYYFQGLDLGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(1767..2627)
                     /locus_tag="JN701_000407"
     CDS             complement(1767..2627)
                     /locus_tag="JN701_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY7362951.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(2985..4064)
                     /gene="fbaA"
                     /locus_tag="JN701_000408"
     CDS             complement(2985..4064)
                     /gene="fbaA"
                     /locus_tag="JN701_000408"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY7362952.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4279..5442)
                     /gene="pgk"
                     /locus_tag="JN701_000409"
     CDS             complement(4279..5442)
                     /gene="pgk"
                     /locus_tag="JN701_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY7362953.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5492..6511)
                     /gene="epd"
                     /locus_tag="JN701_000410"
     CDS             complement(5492..6511)
                     /gene="epd"
                     /locus_tag="JN701_000410"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY7362954.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6883..7314
                     /locus_tag="JN701_000411"
     CDS             6883..7314
                     /locus_tag="JN701_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7362955.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            7337..7915
                     /locus_tag="JN701_000412"
     CDS             7337..7915
                     /locus_tag="JN701_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY7362956.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            7916..8623
                     /locus_tag="JN701_000413"
     CDS             7916..8623
                     /locus_tag="JN701_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY7362957.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRSMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8611..9288
                     /locus_tag="JN701_000414"
     CDS             8611..9288
                     /locus_tag="JN701_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY7362958.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9282..9959
                     /locus_tag="JN701_000415"
     CDS             9282..9959
                     /locus_tag="JN701_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7362959.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(9931..10644)
                     /locus_tag="JN701_000416"
     CDS             complement(9931..10644)
                     /locus_tag="JN701_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY7362960.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10641..11150)
                     /gene="fumE"
                     /locus_tag="JN701_000417"
     CDS             complement(10641..11150)
                     /gene="fumE"
                     /locus_tag="JN701_000417"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY7362961.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            complement(11172..12137)
                     /gene="yggF"
                     /locus_tag="JN701_000418"
     CDS             complement(11172..12137)
                     /gene="yggF"
                     /locus_tag="JN701_000418"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7362962.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(12134..13411)
                     /locus_tag="JN701_000419"
     CDS             complement(12134..13411)
                     /locus_tag="JN701_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7362963.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            complement(13426..14814)
                     /locus_tag="JN701_000420"
     CDS             complement(13426..14814)
                     /locus_tag="JN701_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAY7362964.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSTNAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(14842..15285)
                     /gene="cmtB"
                     /locus_tag="JN701_000421"
     CDS             complement(14842..15285)
                     /gene="cmtB"
                     /locus_tag="JN701_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAY7362965.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHR"
     gene            complement(15599..17590)
                     /gene="tkt"
                     /locus_tag="JN701_000422"
     CDS             complement(15599..17590)
                     /gene="tkt"
                     /locus_tag="JN701_000422"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7362966.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            17896..18627
                     /locus_tag="JN701_000423"
     CDS             17896..18627
                     /locus_tag="JN701_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7362967.1"
                     /translation="MSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQVKTLSDQAC
                     QEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAFAMANGCIRV
                     YSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASAGGIVGSLSQ
                     SQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLAKLEEGRQSS
                     MFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(18833..19753)
                     /gene="speB"
                     /locus_tag="JN701_000424"
     CDS             complement(18833..19753)
                     /gene="speB"
                     /locus_tag="JN701_000424"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY7362968.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(19890..21866)
                     /gene="speA"
                     /locus_tag="JN701_000425"
     CDS             complement(19890..21866)
                     /gene="speA"
                     /locus_tag="JN701_000425"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY7362969.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(21875..22006)
                     /gene="yqgB"
                     /locus_tag="JN701_000426"
     CDS             complement(21875..22006)
                     /gene="yqgB"
                     /locus_tag="JN701_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY7362970.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            22142..22357
                     /locus_tag="JN701_000427"
     CDS             22142..22357
                     /locus_tag="JN701_000427"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7362971.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            22661..23815
                     /gene="metK"
                     /locus_tag="JN701_000428"
     CDS             22661..23815
                     /gene="metK"
                     /locus_tag="JN701_000428"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY7362972.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            24251..25645
                     /locus_tag="JN701_000429"
     CDS             24251..25645
                     /locus_tag="JN701_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7362973.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            25722..26219
                     /locus_tag="JN701_000430"
     CDS             25722..26219
                     /locus_tag="JN701_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY7362974.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            26314..27021
                     /gene="endA"
                     /locus_tag="JN701_000431"
     CDS             26314..27021
                     /gene="endA"
                     /locus_tag="JN701_000431"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY7362975.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            27101..27832
                     /gene="rsmE"
                     /locus_tag="JN701_000432"
     CDS             27101..27832
                     /gene="rsmE"
                     /locus_tag="JN701_000432"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY7362976.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            27845..28795
                     /gene="gshB"
                     /locus_tag="JN701_000433"
     CDS             27845..28795
                     /gene="gshB"
                     /locus_tag="JN701_000433"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY7362977.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            28832..29467
                     /locus_tag="JN701_000434"
     CDS             28832..29467
                     /locus_tag="JN701_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY7362978.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTASADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            29467..29883
                     /gene="ruvX"
                     /locus_tag="JN701_000435"
     CDS             29467..29883
                     /gene="ruvX"
                     /locus_tag="JN701_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY7362979.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(30059..31039)
                     /locus_tag="JN701_000436"
     CDS             complement(30059..31039)
                     /locus_tag="JN701_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY7362980.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            31057..31761
                     /gene="yggS"
                     /locus_tag="JN701_000437"
     CDS             31057..31761
                     /gene="yggS"
                     /locus_tag="JN701_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY7362981.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            31779..32345
                     /gene="yggT"
                     /locus_tag="JN701_000438"
     CDS             31779..32345
                     /gene="yggT"
                     /locus_tag="JN701_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY7362982.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            32342..32632
                     /locus_tag="JN701_000439"
     CDS             32342..32632
                     /locus_tag="JN701_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY7362983.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            32640..33233
                     /locus_tag="JN701_000440"
     CDS             32640..33233
                     /locus_tag="JN701_000440"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY7362984.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            33226..34362
                     /gene="hemW"
                     /locus_tag="JN701_000441"
     CDS             33226..34362
                     /gene="hemW"
                     /locus_tag="JN701_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY7362985.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(34517..35524)
                     /locus_tag="JN701_000442"
     CDS             complement(34517..35524)
                     /locus_tag="JN701_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY7362986.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(35641..36687)
                     /gene="ansB"
                     /locus_tag="JN701_000443"
     CDS             complement(35641..36687)
                     /gene="ansB"
                     /locus_tag="JN701_000443"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY7362987.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(36863..37582)
                     /locus_tag="JN701_000444"
     CDS             complement(36863..37582)
                     /locus_tag="JN701_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY7362988.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(37766..38092)
                     /locus_tag="JN701_000445"
     CDS             complement(37766..38092)
                     /locus_tag="JN701_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY7362989.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(38092..38811)
                     /gene="trmB"
                     /locus_tag="JN701_000446"
     CDS             complement(38092..38811)
                     /gene="trmB"
                     /locus_tag="JN701_000446"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY7362990.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            38972..>39778
                     /gene="mutY"
                     /locus_tag="JN701_000447"
     CDS             38972..>39778
                     /gene="mutY"
                     /locus_tag="JN701_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7362991.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEES"
BASE COUNT         9508 a        10627 c         9947 g         9696 t
ORIGIN      
        1 ccccgcatcc ttgtccgctt aaccgtttca gtcagcctat ccttgatgaa accgcgagca
       61 aagataggtg attacgtcat ggttttacag aaaattacag aaaaaggagg caatatcggg
      121 taaaggcatt agcccgacga atacgtcggg ctacaagtat tattgtgctg caggtgtttt
      181 agcgggttgt tgatccacag gttctaactg gaagaccaca tcgacctgat catcaaactg
      241 aatagcggcc tgctcgtaag tttcctgggc agacaccggc gcggcatcgg ctttcatcat
      301 ccgcaccatt gggctgggct gatagttgga aacatggtag cgcacgctat ataccggccc
      361 cagtttacga tgaaagccgt tcgccagttc ctgcgcctga tgaatcgcgt tatcaatcgc
      421 tgccttacgc gctttgtctt tataggcatc cggctgcgcc acgcccagcg acactgaacg
      481 aatttcgtta agacccgcct tcagcgcgcc atccagcaag gaattcagtt tgtctaactg
      541 acggagcgtg acttccaccg ttctcacagc gcggtagcct ttcaggatac ttttaccatc
      601 ctgataatca taatctggct gggtgcgtaa attcgctgag ctgatatctt ttttcgcgat
      661 ctgattgagt tcaaggaagg aaatgtattg tgcgacgcgc tcatctgcct gtttcttggc
      721 tgtagcggca tccttcgcgg ccacgttaac ttcaatcgca agagtggcaa tgtctggcac
      781 cgcatccaca cttgccgtac cggaggtgac aatatgcggt ccatccggca attcgttagc
      841 ctgcgctgcc atcccgctaa tacccattaa tgccgctagg gcgataactt tgaacttcac
      901 gttagtctct ccgtgttaat tgacttgccc aaaattcagg acctctggag gcaagcttag
      961 cgcctctgtt ttatttttcc atcagatagc gcctaactga acaaggcttg tgcatgagca
     1021 ataccgtctc tcgccagctg caaggctata aaccacatga cacatcccac taccagattg
     1081 ataatgcgct gtgattttgc cgtgcgcagg cgcggtgcca gccaggctgc gagaatagcc
     1141 agaccaaaga accacaggaa agaggcgcta attgtcccga gcgcaaacca acgttttggt
     1201 tcgacgtcaa gctgcccgcc aaggctgccc agtacaacaa aagtatccag gtaaacatgc
     1261 ggattcagcc aggtcactgc caacatggtg gcgataattt tccatctgcc ttgcttcagg
     1321 acttcggcac tagctaactc aatattacta ctcatcgctg ttttaaaagc gccaaaacca
     1381 taccagagca agaagactac gccgccccag gtgaccagcg ccagcaacca cggcgactgc
     1441 atcaataacg cgctaccacc aaaaatcccc gcgcaaatca ggaccaaatc gctgatagcg
     1501 caaagtaagg caatcataat gtggtactga cgacgaatgc cctgattcat cacaaaagca
     1561 ttttgtgggc cgagcggcag aatcatagct gccccaagat caagaccttg aaagtaataa
     1621 gaaaacacgt tggatattcc gaattaagta tctgatgcag agactatatc gcgggattgt
     1681 tattagcgga aattgataat attaattggc aatcagaata actaataaga gagaggccag
     1741 agagtattat ctggcctcag cgttgattac gcagctttgt cttctttcac ccgcttaaag
     1801 ttcacatcca tttgcgggta cgggaagctg ataccggcgg catcaaattc acgtttaata
     1861 cgctccagca catcccagta cacgttttgc agatcgccgc tgttgctcca gacgcggacc
     1921 acgaaattaa tcgacgatgc accaagttcg ttcaggcgca cagtcatttc gcgatctttc
     1981 aaaatgcgat cttcagactg gataatattg gtcaggatct gcttaacctg atcgatatcg
     2041 gaatcatacg ccacgccaat aataaattcg ttacggcgaa ctggctcgcg ggagaagtta
     2101 ataatatttc cggcaataat tttaccgttc ggaataacga taattttacc gtctgcagta
     2161 cgcatggtgg tggagaaaat ctgcacactc agcacagtac cggctacgcc gccgaggtca
     2221 acatattctc cggcacggaa cggtcggaac atgacaagta acacgccagc ggccaggtta
     2281 gaaagtgacc cctgcaaagc cagaccaaca gctaagcctg cggcaccgag tacagcaatg
     2341 actgacgctg tttgtacacc cacgcgtccc agggcagcga ttagcgtaaa ggcgataata
     2401 ccgtaacgga ctaatgcaga aagaaaatca gcaacagtgg catcgatttt acgggagatc
     2461 atcaggcgat tcaccgcgtt ggaaatcatc cgcgcgataa tcaaaccaac gatgatgatc
     2521 gcgagtgccg ccacgatgtt tactgcataa cttagcagca gcgcctggtt agctaccagc
     2581 cagcttcccg cgccgtttat gctatcgaca acattcaaat cttccattca atattccttt
     2641 tcaactgact caaaatggag aaatacactc cgccgcctta tgacgggcag tctgacaagg
     2701 gtaaacaata aagcataatt ttgccaaata ggtcacagat ttctcatctg aatcaccttg
     2761 cttttggcga aaaaatcccc gcagagcggg gctcagacag atatcaatgt gcgctttgtc
     2821 atgccggatg cggcgtgaac gccttatccg gcctacaaaa acctgcaaat tcaatatatt
     2881 gcaggatacg tgtaggcctg ataagcgtag cgcatcaggc aattttgctt ttggcgaaaa
     2941 aaagacccgc agagcgggcc ttgagataag cagaaaggaa tatcttacag aacgtcgatc
     3001 gcgttcagtt cctggaatgc tttttccaga cgagcgatca tcgaagtctg accggcacgc
     3061 agccatacgc gcggatcgta gtatttcttg ttcggctgat cttcgccttt cgggttaccc
     3121 agctgaccct gcagataagc ttcgttcgct ttgtagtagt tcagaacgcc ttcccaggtt
     3181 gcccattggg tatcggtatc gatgttcatt ttcactacgc cgtagcttac ggagtctttg
     3241 atttcctgag cagtagaacc ggaaccaccg tggaatacga agctcaggct gttgtgcggc
     3301 aggttgtgtt tcttagaaac atattcctga gaatcacgca ggatggtcgg agtcagaacc
     3361 acgttacccg gcttgtaaac accgtgtacg ttaccgaagg acgcagcgat ggtgaaacgc
     3421 gggctgattt tgctcagctc ggtgtatgcg taatcaacgt cttccggctg ggtgtacagt
     3481 gcagaagcgt ccatgtggct gttgtccacg ccgtcttctt caccaccggt gcaacccagt
     3541 tcgatttcca gagtcatgcc gattttggac atacgctcca ggtatttaga gcagatttcg
     3601 atgttctctt gcagagattc ttcagacagg tcgatcatgt gagaagagaa cagcggctta
     3661 ccggtagctg cgaagtgttt ttcacccgcg tccagcagac cgtcgatcca cggcagcagt
     3721 ttcttcgcgc agtggtcagt gtgcaggata accggaacac cataatgttc agccatctgg
     3781 tgaacgtgat gcgcaccaga gatcgcgccc aggatagcag caccctgcgg aacgtcagat
     3841 ttcacgcctt taccagcgat aaaggaggca ccaccgttgg agaactgaac gataaccggc
     3901 gctttaactt tagcagcagt ttccagtacg gcgttgatgg agtcagtacc gacgcagttt
     3961 actgctggca gtgcgaagtt gttttctttt gctacctgga aaactttctg tacgtcatca
     4021 ccagtgatta cgccaggttt tacgaaatca aaaatcttag acatgtctct tgtcctgtat
     4081 cgtcgggcct tatactcatc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     4141 ccagtcactt acttatgtaa gctcctgggg attcactctc ttgtcgcctt cctgcaactc
     4201 gaattattta gagtatgaaa aaatgcgtgc tctaaaagcg cgctgaaaca agggcaggtt
     4261 tccctgccct gtgatttttt acttcttagc gcgctcttcg agcatcgcta ctgcaggcag
     4321 tactttacct tccacgaatt cgaggaatgc gccgccgcca gtggagatgt aggagatttt
     4381 gtcagcaatg ccgaacaggt cgattgctgc cagagtgtcg ccgccgccag cgatggagaa
     4441 cgcttcgctg tctgcgatag cgttagccac gatttcagta cctttgcgga agttcgggaa
     4501 ttcgaacacg ccaaccggac cgttccacag aatggttttc gcattcttca ggatttcagc
     4561 cagttcctga gcggaagcat caccgatatc caggatctgc tcgtcagctt tcacatcgtt
     4621 aacagatttc agggtagccg gtgcagtttc agagaactcg gttgctacgc gaacatcaga
     4681 cggaaccggg atgttgcagg tggtcagcag acgtttagct tcgtcaacca ggtcagcttc
     4741 gtacagggat ttacccacat cgtggccttg tgccgcgata aaggtgttag cgataccacc
     4801 accaacaatc agctggtcag cgattttaga cagggagtcc aaaacggtca gtttggtaga
     4861 tactttagaa ccaccaacga tagccaccat cgggcgagca ggttctttca gtgctttacc
     4921 cagcgcgtcc agttcagctg ccagcagcgg gcctgcgcac gcaacgtcag cgaatttacc
     4981 gataccgtga gtagaagcct gcgcgcggtg agcagtaccg aatgcgtcca ttacgaacac
     5041 gtcacacagt gcagcgtatt ttttggacag ggtttcgtcg tctttcttct cgcctttgtt
     5101 gaagcgaacg ttttccagaa caaccagttc accttcagca acgtcaacgc cgtcgaggta
     5161 atctttaacc agacgaaccg ggttagacag tttgtctttc aggtagttaa caaccggcag
     5221 cagagagaat tcttcgttgt actcgccttc ggtaggacga cccaggtggg aagttaccat
     5281 cacttttgcg ccttgtttca gggccagttc gatggtcggc agagaagcac ggatacgcgc
     5341 gtcgctggtt actttcccgt cttttactgg tacgttcaga tccgcacgga taaatacacg
     5401 tttcccagca agatccagat cggtcatctt aattacagac atggtgaatc ctctcgttga
     5461 ttctaaaagt tttgcagacg ctgcttgcgt cttacctgaa agcaacagta gccatagcta
     5521 acgtcgtgtc gagcattcgg ttagcaaagc cccattcgtt atcgcaccag accaacgttt
     5581 tgatcaggtg tgcgccactg acccgggttt gggtgccatc gacaatggca ctgtgcggat
     5641 cgtggttaaa atctacagag accaacggca attccgtata gtcaactata ccatgaaatg
     5701 caccttgtgc tgctttttgc agcaacaggt tgacttcatt ggcttttaca ggtttcttca
     5761 ccgtcacgct taaatcgatt gccgtcacat ttatggttgg cacacgtacc gcaatcgctt
     5821 caaagcgatc gttaaattgc ggaaaaaatc gtgtgatacc ggcggccagt ttagtatcga
     5881 ccggaatgat cgactggctg gctgcccggg tgcgacgcag gtcaggatgg tatgcatcaa
     5941 taacctgttg atcgtgcatg gcggagtgaa ttgtggtcac agtgccggac tcaataccgt
     6001 acgcatcatc taacaatttg atgacgggaa ttatgcaatt cgtggtacag gaagcgttag
     6061 aaacgatgcg gtgttccgca cgaagttgat cctgattgac gccgtaaaca acggtcgcgt
     6121 cgagatcgtt actgccagga tgtgaaaaga gcactttttt ggccccggcg gcaatatgcg
     6181 cttcgccatg ctcgcgggag ccatatacgc cggtgcagtc gaggactaca tcaacgccaa
     6241 gttcacgcca ggggagcgat tgcagtgaac gctcatgcaa tacgcggatg gcgtcatcac
     6301 caacaaaaag ttgatcgcgt tcctgtcgta cttcccatgc aaaacggcca tggctggtgt
     6361 catatttcaa caaatgcgcc atgcccgcag catccgccag ttcgttgatt gccaccacgg
     6421 taatttccgc ccggcgtccg gattcataca aagcacgaac cacattacgc ccgatgcgac
     6481 cgaagccatt tatcgctacg cgtacggtca tagatctcct gcaaggtttt ccctgagcaa
     6541 atttgccaga cagagtaatc cagcaaatcg tccggggaaa ccttacctgt cgcaaactgc
     6601 gactgattgg ttaattgtcg aacatttaat cgactgaaac gcttcagcta ggataagcga
     6661 aacgtggaat aaaaggaatg attgtccagt caaagaaagc aattatctga cttacgtcac
     6721 gttttcagcc ccgcccctgt agaatttatt ccactttgca gaatcacgaa taagcgtagc
     6781 aaaatctttt cgttgtgccg tcacaaaccc tgacttatat cagaatatga ctgatgcagc
     6841 gtcgatatta tccgcaatac gtccgagttt cggagtacta caatgatgac attgccctac
     6901 ccatattctt cctccgcacg cttttacgca ctgcgcctcc tccccgggca ggaagttttt
     6961 tcccagctac acgcttttgc acagcagcat caacttcatg ccgcctggat tgcaggctgt
     7021 accggtagcc tgactgacgt cgccttgcgc tatgccaggc aagaaggcac cacgctcctg
     7081 aacggtacat tcgaagtgat ctcactaaac ggcacgctgg aacagagtgg tgagcatctt
     7141 catctctgcg tttccgatcc acacggcacg atgcttggcg gtcatatgat gcccggctgt
     7201 accgtgcgga ccacgcttga actggtgatc ggtagccttg aggaactgga gtttagccgc
     7261 caaccttgcg cgctttctgg ttacgacgag ttgcatattt cccccgttaa ataactactt
     7321 tcaaagaggt tttcttatgg cgcgtagcca cttttccagt caggcactgg tgttgatcgt
     7381 tatatctatc gccatcaata tgatcggcgg gcaactggcc agcatggtca aattacctat
     7441 atttctcgac tccatcggca cacttattag cgcggtactg cttggtccgg tgatcggaat
     7501 gctgaccggc ttactgacca atttactctg gggattgctg accgacccga tcgccgccgc
     7561 atttgcaccc gtcgcgatgg tcatcggcct ggtcgccggt tggctggcgc gagcgggttg
     7621 gtttcgcacc ctgccaaaag tcgttgtcag cggcgtgatt attacgcttg cggtaacggt
     7681 cgtcgctgta ccgctgcgta ctgcgctgtt cggcggtgtc accggtagcg gagccgattt
     7741 gttcgttgcc tggatgcact cgatggggca aaacctggtg gagtcggtgg cgattacggt
     7801 gataggcgcg aacctggtcg ataaaattct tacagcggtg attgtctggc ttttactgcg
     7861 ccagctgccg attcgcacca cgcgccattt tccggcaatg gctgccgtgc gctaaatgca
     7921 tccatttacc tcattaacac tgtgggcatt ggcggcctgc accacgctta tcctgcccgc
     7981 gcagaccatt ttgccgatct atagcgcggc gacctttttc tgccttattg cgctaaaggc
     8041 cacgcggcgg cgggcaaaat atgtcgtctg gctgatgttc tcgctgggag ccgggctgtg
     8101 gctggtacac ggtggctggc taccagaatg gctaagcggc acgccacgct cgccagaacg
     8161 gtggtcgcat gctattacgc tctggttgcg gattctggcg attgtttcaa cctcacagtt
     8221 gtggatgcag tacgtccccg tgcaatgttt tattcgcgct ctgtttgctt ctcgcctgcc
     8281 gccaggcgtc gcttatctgt ttgccgggcc gttattggtc gttgagcagt taaaacggca
     8341 attagcgatt attcatgaag cgcagcgtgc ccgcggcgtg ccgctggatg agggctggta
     8401 tcagcgttta cgttcgatgc ccgcgttaat tattccgcta acacacaatg cgcttaacga
     8461 tctggctgtc cgtggtgcgg cgctggatat gcgggcattt cggatccaca accgacgaac
     8521 aacgttgtgg gcaccagccg atagcaccct acaacgcgta gcgcgttaca ccatgatctt
     8581 gctgatgctg acagaattcg gagcgtggat atggttacgt tagaacagtt ccgttattgc
     8641 ccgacacact caacgcatcc tccgttctgt tatgactttc actatgtcaa accggggatg
     8701 gtcgccatct ttggtgacaa cggcagtggc aaaagtacgc tggcacagct gatggcgggc
     8761 tggtatccgg actttctgcc aggagagata acaggcacgg ggacgctcct cggtacgccc
     8821 attgggcatc tcccgcttaa tgaacaatcc gccaccatcc agctcgtcca gcaatcgccc
     8881 tatttgcagc tctccggctg tacctttagc gtggaagaag aagttgcctt tgggccagaa
     8941 aatctgtgtc ttgctgaagc agagattatg gcgcggattg atgccgccct gactctgacc
     9001 gagtgccagc cgttgcgcca tcgtcacccg gcaacactct ccggtggaga gacacagcgc
     9061 gtggtcatcg cctgcgccat cgcgatgcag ccaaagctct taattcttga tgaagccttt
     9121 agccgcctga cgccgcaggc cagcgaaatg ctgttgcagc gcttacaaca ctgggcgttt
     9181 gaacgtggtt cgctgattat tctttttgaa cgtcatcgca cacctttcct gaactactgc
     9241 cagcaagcgt ggcaattgca aaacggagcg ctccagccac tatgttaacg ttaaatcaga
     9301 tatcttatcg ctggcctggc gcggcaacgg attgtctttg cgacatttct ctgcaactca
     9361 aacaaggtga atggttggcg ctgaccggtg acaatggcgc agggaaatcg acgctgttac
     9421 gagtaatggc aggtcttctt acgcccacag ccggtacggt tatgctgcaa caacaggcga
     9481 tgaaaaacct gaaaaaccgc cagcgcgcgg caaaggttgg cgtactgttt caggaagcgg
     9541 aaaaccaact ttttcacagc accgtcgctg acgagatcgc atttggtttg aaactgcaaa
     9601 aatgcccggc ggatgaaatc actcaacgta cccatgctgc cctgcaatgt tgtcagttag
     9661 cagacactgc caactcgcac ccgttggatc tgcatagtgc gcagcgacgg atggtcgccg
     9721 tcgcctgcct ggaagcactc tctccgccac tattactgct ggacgagccg agtcgggatt
     9781 ttgacgaaaa ttggttgagc gtgtttgaaa gctggctgga gaagtgccgc cagcgaggca
     9841 cgagcgtggt agcaatcagt catgacgccg catttacccg gcgtcatttc tctcgtgtgg
     9901 tgcgactgga agatggtgta atcaggaacg ttaatccacc agacgatatt cacccgtagc
     9961 ggtcatcatt aaggtcaaat ttgcaggcag actctcttcc agcacccggc gaacattcgg
    10021 cccgtcggta cggtcataaa aggcttctgc atctgccagc gatacccctc ccgccagctt
    10081 gcgccccacc agacgctccc gcaacgcagt agctggcgcg ttgataaaga tcgagaaatc
    10141 acaaaattgc gcaagctggc accacttctc gtcgtccagc aacaaccagt ttccttcgac
    10201 gatgacgagt ggtgcggtaa cgtgcaacgc atcttcaaca ggatcatgct tttgtcgatc
    10261 gtactgcggc cacgtacaat ccccttccac cacctggcgc agattttccg ccagtttcgc
    10321 aacgtcaaat gtctctggtg cgcctttgaa ggggcgcaat tgatgcgcgt ccagccagct
    10381 attgtaatgg tgaaaaccat ccatcgggag cgtctgaata gcgggcagct ccgggtcttg
    10441 ttgcgcgaga tattcccaga aggtggttag ggtagattta cccgtccccg gcggagcgca
    10501 aagaaaaacc accgtacgcc gctgcggatt taccgtctgt aaggccgcca gcatacgcag
    10561 caacggttta tgtacatttt caatctcttc gttctggtac tgtgcctgta tcttcagccc
    10621 attcaccgtt aattcaattt tcacggcgtt aattccttta agattgtcgt tactgccagc
    10681 gtcagcgccg ttttgaccat cccgctaaaa cgcgcctctg accagacggc gaaatcggcg
    10741 aacttcatcg ctttcaacgc gtcatagagc atcgcattgc gggttatgct attgacgttg
    10801 ctgataaaat cccaggtcat gtcatcggca aatccgggcg tttcatcctg ttcgttcagg
    10861 taatgccaga actgggaaaa atgcgtgata tcggcgtaca gccatttatc cattttcccc
    10921 agcgcataaa tcaaacgcag cgcaacatca atatcgtcaa gcgggccgct ttgggcgagt
    10981 aacggcttca ctgcatattc cacgatctct tcatcgttat cgacaaacag cgacggcaga
    11041 aactggcgta tcccctggag caaaatagaa tgcgcagttt tcataaatga aaataaattg
    11101 tcctgtgcat ccagttgctc aagcacatca tcttctgtca gcttcgccat tggtcttccg
    11161 atagatcacc atcaatgcag ggagtctatt atattacacg tttggtccgc gccgccgatc
    11221 agtaatgtct gcgtccgcac cccattcgcc gtctgttgga taccgttcac caactcgccg
    11281 cccgtcacgc ccgtagcgct aaagaggata tcgttccccc tcaccagttc atcgagcgag
    11341 tacacacggt taacgtcaac gcccattgcc ttgcaacgct tgctctcctg ctctgcaatc
    11401 tgccgatttt ccgtgtaatc ccctttagcc tgacaaaagt caatcagctc ggcctgcata
    11461 tccccgccta atgctttaac ggcacaggca gaaatcacgc cttctggcgc accgccgatg
    11521 gtgtacatca catcgtaagg attatcctgc cagcaggtca gcacgctggc ggcgacatcg
    11581 ccatccggca gggcaaaaac cttcacgccc agctgtgttg cttcctcaat cgcggcactc
    11641 aggcgcggtt tatccagcgt aaccatgcgc agcttgtcca gcggtttacc taacgcccgc
    11701 gccacgttac gcaagttgtc cgccagaggc aaagacagat caatcacccc ggcagccagt
    11761 cgattaacaa ccagcttttt catatacata tcgggcgcat gtaacaggct atcccgaggg
    11821 gcgaaagcca tgaccgccag cgcattgctc tgccccatcg ccaccatccg cgtgccttca
    11881 atgggatcaa ccgcgatatc gacttctggc ccatcgcctt tgcccacctc ttcgccaatc
    11941 cacagcatcg gcgcatggtc aatttctcct tcgccgataa ccactcgtcc acgaaaagcg
    12001 acgtcgttta atgcctggcg catcgcggtg accgccaggc catcaatttt atttttgtcg
    12061 ccacatccgg tttgcggcca ggcagccaac gctgcctgtt ccgtaacgcg aaataatggc
    12121 cacgccaggg acatcattgc gcggcctccc cgatatcaat gccaaattgt gccagcagat
    12181 atttctcggc ctgttcattc cagatgccgt gcgtttcctc aaccagccgc gccaactctt
    12241 taaatagcgg atcggttttg cctttttcgg caaaatcggc aatggcagtg agcggcatgg
    12301 tcacgccgtt ataaatgagt tttttaccgc cagggatatc cggcagattg agcacggtat
    12361 ctggcaccgc atccagccca ccgatatgcg tgaccataaa ggacggctgc aactgccccg
    12421 tggcgcttaa cgcaatcgcc tctttcatgt cgtccgttga accgccggat gtaccgacta
    12481 cgtgcgtgct gttgtaatgg acgttgtaga aattaaacgg cactttgaag tttttatccg
    12541 tcggcccggc aaagaagttc agacagccat cttccgccag taattcgtca gccatctcaa
    12601 cgacagcagg caccgccgca taaacaaaaa tgtcatcgaa cccgacatct cctgtcagcg
    12661 cccgcagcgt ctggaccgga tcgctcatcc ctttggtatt cacatacacc agctcaatgc
    12721 ctttactggc cgccagatcc accggcagca gcttctgcac ctgcgccagg cgtttgtcgt
    12781 cgatatcgac caccaccacc cgtgacggtt gtatgccacc gttaatggcg taatcgatag
    12841 cgccaatgcc catcggaccc gcacacgcca gcagtgcaat attgccgcca ggtttgacgc
    12901 ccatgcgatg ctcataaaca tattgcgtgg tgtgataatt ggcatgataa gcaccaataa
    12961 tgcagcacat aggctctacc agcgacgccg cagcaaaata agagccgtga tacggtaata
    13021 cgcagcccaa attaatggcg atttcgggaa taatcatata ggtggcgtta ccgccaaaat
    13081 attcgtagct gtagcctgct gaatatccgc ttggtaaccc catcgccggt tgcaatacaa
    13141 aacgctggcc ttttttatat ttgccagtga gatttttacc cacttcgaca ataaccccgg
    13201 cacattcatg cccggtaatg accggatgat tttctaaatc gtcgggtacg cgtttatgtt
    13261 cactaccgag taacgccgct ttccaggtcg ataaacagac gctgtcagaa attacactca
    13321 ctaacagttc attatcggta atttctggca gttcaaattc gcgcagacgg acatcccgct
    13381 tgccataaat agcagcaact ttggttttca tttttacctc tgtaatcagt gtttatgttc
    13441 ggcggttaat tgattaaata aagtgtcgag ttttggatcg ccaatatagt tattaatcaa
    13501 tatcagcggt ttatccgtca cacgtttcac gcgcccttcc agactggcgt gagtgacgac
    13561 gatatcggca tccgcaggca cgttttctat cgcgtaatgt tttacttcta ttgccagccc
    13621 cgctttttcc aggcgtttac ggaaggtggt cgcgcccatc gcactggagc ccatccccgt
    13681 atcgcaaaca aaggcgatac gcttaaccct gcttaacgag aatgcaccct cttgtttcat
    13741 cgccttaacc gcattggttg actgagcaaa ctcatcttcg ctctccgttt ccaccgtttt
    13801 ttccatcttc agtatcagcg aagtaatagc aaaggacacc agggtaccta ccgtaacacc
    13861 ggcaattgtc gccaggaacg agcctttcgg cgtcagtgcc aggtaagcaa agatagaccc
    13921 cagacttggg ccagccacca gaccaccgtc cagtaagtta aacatccagg taccagacat
    13981 accgcccgca atcatggcaa taatagtcag cggcttcatc agcacatatg ggaaatacag
    14041 ttcgtggatc ccaccgagga agtgaataat catcgccccg ggcgcagaac gtttactcat
    14101 ccctttacca aacaaggtaa acgccagcag cagccccagg cccggacctg ggttagaggc
    14161 caccataaag aagatggatt taccgttaac cgaagcctgt tgcattccca gcggataata
    14221 gacgccctga tcgatcgcat tattaaggaa aagcactttc gccggttcat tgatgactga
    14281 cagcagaggc agataacccg catgtaccag cgcctcgata cactctttga cgaaagtatt
    14341 ggcaattaac accgccgggc cgatcacttc aaaacccagc agacaaagga gcatccccgc
    14401 gatacctaat gagaagttat tgataaccat ctcaaaaccg gcaggtatgc gtttttccag
    14461 tgccttatcg acatatttta tgaccaaccc accgagcggc cccatgatca ttgagccaag
    14521 gaacatcggg atctctgcgc caacgatcac accaatagta cctattccgc ccatgaccgc
    14581 cccgcgttta ccgccgacca gatgaccacc tgtagaacca atcatcacgg gcaataaata
    14641 ggtaatcatc gggccgacaa ttttggcgaa atgttcgtta ggcagccaac cggtgggaat
    14701 aaataacgca gtaataaaac cccaggcaat aaaagcacca atattgggga tgaccattgc
    14761 agtcaaaaag cccccaaaag cctggacctt tgcacgagca gacttgtttt ccataatatt
    14821 atcctgtaga ggagagagta attatctgtg gctgataatg tccgctaatt gtttttctga
    14881 tgatgctgtt aagagttgtt cgagtatttc ttcttcgcac agtaattcac ttaacgcctg
    14941 aatagcgcca atgtgcgaat cggcatcggc agcagagagt ccgatgagta atttaattgg
    15001 ttcgtcattc cccggaaaat aaacaccttg ttcaagtaat gtcaaagaca tcccggtttt
    15061 aagcgcccca cattccggtc gcgcatgagg cattgccacg cctggtgcga gaatataata
    15121 agggccattg ttaatggtgg aatctttaat tgcctgaatg taattctcgc tgatatagtt
    15181 tttatccagc aatgatacca tcgagaaatc gatagcttcc tgccaatctt ttgctgaatg
    15241 tataactgag attgatgatt ctggaaaata gtcactaagc cgcataaatt atccttattt
    15301 tatttttagg gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta atgaaagcaa
    15361 ttgagcaggg aaaccggtgt tttgaaaaca gtgaattatt aatttcttat ataacattga
    15421 gttatagata taacaacaaa tgtcaatacg catatcgtga tgcatattac ataattgata
    15481 tttattgcat ataaatattt gtgatctgca acacgcttta tctattgctt gtccgcaaac
    15541 ggacatatca aggtaataaa aaaggtcgcc gaagcgacct ttttacccga aatgctaatt
    15601 acagcagttc ttttgctttc gcaacgacgt tgtcgacggt gaagccgaac tcttcgaaca
    15661 gcaactctgc cggagctgac tcaccgaagg tggtcatacc gatgatagcg ccattcaggc
    15721 caacatactt gtaccagtag tcagcaatac ccgcttctac cgcaacgcgt gcagtaaccg
    15781 ctttcggcag tacggattca cggtaagcag catcctgctt gtcaaatgcg tcggtagacg
    15841 gcatggacac cacgcgcgct ttcacgcctt cggcagtcag tttttcgtag gcagcaacag
    15901 ccagttcaac ttctgaaccg gtagcgatga aaatcagttc cggctgaccg gcgcagtctt
    15961 tcagcacata accaccgcgc gcgatgtttg ccagttgctc ttcagttcgt tcctgctgcg
    16021 ccaggttctg acgggagagg atcagtgcgg tcgggccgtc ctgacgctca acaccgtatt
    16081 tccacgcgac cgcggattca acctggtcac acggacgcca tgtagacatg ttcggggtta
    16141 cgcgcagaga agcgacctgc tctaccggct ggtgagtcgg gccatcttcg cccagaccga
    16201 tggagtcgtg ggtgtaaacc atcacctgac gctgtttcat cagcgcagcc atacgtacgg
    16261 cgttacgtgc gtattccacg aacatcagga aggtggaggt gtacggcagg aaaccaccgt
    16321 gcagggagat accgttagca atcgcggtca taccgaactc gcgaacaccg tagtggatgt
    16381 agttacccgc agcatcttcg ttgattgctt tagaaccaga ccacagggtc aggttagacg
    16441 gtgccaggtc agcagaaccg ccgaggaatt ccggcaacag cggaccgaaa gcttcgatag
    16501 cattctgaga cgctttacgg ctggcgattt tcgccgggtt agcctgcagt ttagcaatga
    16561 actcttttgc tttcgcgtcg aagtcagacg gcatttcgcc tttcatacgg cgggtaaatt
    16621 cagcggcttc ctgcggataa gctttcgcgt aagcagcgaa tttctcgttc catgcggatt
    16681 ctttcgcctg gcctgcttct ttcgcatccc actgagcata gatttcagac gggatttcga
    16741 acggtgcata tttccagccc agctgttcgc gggtcagggc aatttcagcg tcgcccagcg
    16801 gcgcaccgtg ggagtcgtga gtaccggctt tgttcgggga accgaaaccg atgatggttt
    16861 tgcacatcag cagggacggt ttgtcagtca ctgcgcgcgc ttcttctact gcgcgtttga
    16921 tggatgccgc gtcatgaccg tcgatgtcgc gaataacgtg ccagccgtaa gcttcgaaac
    16981 gcattgcggt gtcgtcggtg aaccagcctt caacgtgacc atcaatagag ataccgttgt
    17041 catcgtagaa cgcaatcagt ttacccagct tcagcgtacc cgccagagag caaacttcgt
    17101 gggagatgcc ttccatcatg cagccgtcgc ccatgaaggc gtaggtgtag tggtcgacaa
    17161 tgtcgtggcc cggacggtta aactgcgccg ccagcgtttt ttctgcaatc gccataccga
    17221 ctgcgttggc aataccctga cccagcggac cggtggtggt ttccacacca gcggtgtaac
    17281 ccacttccgg gtggcccgga gttttagagt gcagctgacg gaagtttttc agttcttcca
    17341 tcggcagatc gtaaccggtg aggtgcagca ggctgtagat cagcatggag ccgtggccgt
    17401 tggacagcac gaagcggtca cggtcagccc aggacggatt ctgcgggttg tgtttcagga
    17461 aatcacgcca caggacttcg gcaatgtcag ccatacccat aggggcaccc gggtgaccgg
    17521 atttggcttt ctgtactgcg tccatgctca gcgcacgaat agcattggca agctctttac
    17581 gtgaggacat tttgactcca gatcggatga tgaagggcac gcccttaacg acttgacgac
    17641 agcgcgtttt gggctacgcc ggaaaatttg ccaacaattt accgcaagcc gcgcgtcatg
    17701 tacatggaac atccttttgc cgcttcagaa atctctggat catgctcgca tgttgcgcaa
    17761 tctactcgcc cgtccgctgc gcttttcctt atactgagac tgagcgtcga ttcacctgca
    17821 aacggcgcat ttttagaata atcctgacct tgtacggaag agaaaacatg aaaattcgcg
    17881 cctttattgg tagcaatgag cgtggcaacg gtactgactg gttgccagaa tatggactcc
    17941 aacggactgc tctcatcagg agcggaagct tttcaggctt acagtttgag tgatgcgcag
    18001 gtgaaaaccc tgagcgatca ggcatgtcag gagatggaca gcaaggcgac gattgcgcca
    18061 gccaatagcg aatacgctaa acgtctgaca actattgcca atgcgctagg caacaatatc
    18121 aacggtcagc cggtgaatta caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg
    18181 gctaacggct gtatccgcgt ctatagcggg ctgatggata tgatgacgga taacgaagtc
    18241 gaagcggtga tcggtcacga gatggggcac gtggcgttag gccatgtgaa gaaagggatg
    18301 caggtggcgc ttggtacaaa tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc
    18361 ggcagtttat ctcaatcaca acttggtgat ctgggcgaga aattagtcaa ttcgcaattc
    18421 tcccagcgcc aggaagcaga agccgatgat tattcttacg atcttctgcg ccaacgcggc
    18481 atcagcccgg caggtcttgc caccagcttt gaaaaactgg cgaaactgga agaaggccgc
    18541 caaagctcaa tgtttgacga ccatccggca tccgccgaac gcgcccagca tattcgcgat
    18601 cgcatgagcg cggatgggat taagtaaagc ctggtggtgt cgaacgtagg tcagataagg
    18661 cgttcacgcc gcatctgaca ttaatggcac atttgttacc ttgtgcgcat tgccggatgc
    18721 gatgctggcg catcttatcc ggcctacggg tgccgaacgt aggtcggata aggcgttcac
    18781 gccgcatccg acattaatgg cacgttttac ccgtgcgcat cgcatctggt gcttactcgc
    18841 cctttttcgc cgcctgaata tacagcatct ccagcgccag cgtcgccgca gccagagcgg
    18901 tgatttccga ctgatcgtat gccggagcca cttccactac gtccatccca acaatgttga
    18961 gatctttcag gccgcgtacc agtttaatgg cgcgatcgga ggtcaggccg ccaatcactg
    19021 gcgtaccggt tcccggcgcg aaagcaggat ctaggcagtc gatatcaaag gtcaggtaaa
    19081 ccggcatatc acccacaatc tgtttcactt gggcgataac gtcatccacg ctgcgatcgt
    19141 tcacctggca ggcgtccagc acggtaaaac cgttgtcttt atcaaactcg gtacgaatac
    19201 caatctgcac ggaatgattc gggtcgatca gaccttcttt cggcgcggta tagaacatgg
    19261 tgccgtggtc aaattcacaa ccgttcgcgt aggtatcggt gtgggcgtca aagtgtacca
    19321 gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag cggcagcgta acaaagtggt
    19381 caccaccgaa agagagcata cgcttaccgg cagccagcag cttctcggcg tgtgcctgca
    19441 gtttttcgct catctcacgg gcatcgccaa aggcatatac cagatcgccg cagtccacga
    19501 cgttcaggcg ctcacgcatg tcgaaattcc acgggaagcg attgtgttcc caggcaagat
    19561 tcgtcgaaac ctgacggatc gctgccggac cgtggcgtcc ccccgcacga ccagaagtgg
    19621 ccatatcgaa cggcacacca gtaatcaccc agtcagcatc gctgtcatac ggctggaagt
    19681 tcatcggcag gcgtaaaaaa ccaaaggcgt tggaaaccag tgagttatcg tattgatgac
    19741 ctaaggtgct catggatgga ctcctattac aaagtcgata taaaaaaacc cttccgcgtc
    19801 gttaggcccg acgaggaagg gttggatttg tcacaataaa ttgtggcgga ttatcaccgc
    19861 taaattaagc ggattcaagt aatacggact tactcatctt ccagataagt ataaccgtac
    19921 aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc
    19981 ttcacttgat cgcggaactg agttaacagc gttttcggat cgagctgcac atattgcagc
    20041 atatccgcaa cggtatcgcc ttcgtcagac agttccactt ctacactacc gtcagggaag
    20101 acgaacacgt caaccgcttc ggtatcaccg aacaggttgt gcatgttgcc gaggatctcc
    20161 tgatatgcgc cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc
    20221 attggcattg tcgtggcaat accatcacca tcaatatagt ggtcgatagc accgtcagag
    20281 tcacaggtaa tatccagcag cacagcgcga cgttccggca cttgatccag cccttccagc
    20341 ggcagaaccg ggaacaactg gtcgatcccc catgcgtccg gcattgactg gaacagcgag
    20401 aaattgacgt acattttgtc cgccatacgt tcctgcagtt cgtcgattat cggacgatgc
    20461 gcacggtttt gcggatccag ttgcttctgc acttcatggc acatgctcag atagagttgt
    20521 tctgcccatg cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg
    20581 tgcagatcca tctgactgtc gtgtaaccat tcacgcagag aacggcgagt tcccggttcg
    20641 tgcatctcct gccaggtttc ccacatgctt tgcagcgcgc gcggcgcatc ttctgcaggc
    20701 gcggtcggca ccgtgtattc gttacgttcc acgccgatga tattagacac cagcacggtg
    20761 tgatgcgcag tcaccgcacg acccgattcg gtgattaccg tcggatgcgg cagaccgttt
    20821 tcttcacacg catcaccaat cgcccagatg atgttgttgg catattcatt gaggccgtag
    20881 tttacggagc agtcagactg cgaacgggtg ccttcatagt ccacgcccag accgccgccg
    20941 acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat
    21001 tcacgaacgc ccgtggcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc
    21061 agtagttgca ggctgtcgag acgcccagct tcacgcagcg tttcaaccag ttgcagtacc
    21121 tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aagactgcca tttacccgag
    21181 ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct
    21241 tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc
    21301 atcttctcgc caattaatgc caggcggata tattcgcggt ctttataacc gttgcagacg
    21361 atgacgctac gggtcatgcc agcatgtgcc agcactgcca tcaactcggc tttggaaccg
    21421 gcttccagac ccagcggttc gcccgaatga atcagggact caatcacgcg gcggtgctgg
    21481 ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga ttccctcgca
    21541 cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac
    21601 agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg
    21661 cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca
    21721 tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg
    21781 gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc
    21841 aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac
    21901 agttaacgac tatcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca
    21961 gcagtgaatg ctgacgctcc aactgcgcaa ccggtttctt tttcataaca ttattaagca
    22021 cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa
    22081 gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga
    22141 gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgaatctt
    22201 caccagaacg gtgagacagg ttaagcagca gactacggtt cattatttcg tatcacctcc
    22261 acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt
    22321 ggcaacacga aagaaacgcc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc
    22381 cacaatggca gaaaattgca aaatataaat acgcagaatg ccggcattgt cagaaaaaat
    22441 ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa
    22501 gaattgacct aaaatagcca tctagatgtt aatccatcca taccgattaa cactcagact
    22561 gccagtgttt ttaacctgca gagtcgtggt aggatccgct accacagaaa atccacacaa
    22621 cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac
    22681 gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt
    22741 tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa
    22801 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga
    22861 gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc
    22921 taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca accagggcgt
    22981 tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc
    23041 aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca
    23101 gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag
    23161 ccaggtgact ttccagtatg acgacggcaa aatcgttggt attgatgctg tcgtgctttc
    23221 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat
    23281 catcaagcca attctgcccg ctgaatggct gacttctgcc accacattct tcatcaaccc
    23341 gaccggtcgt tttgttatcg gtggcccgat gggtgactgc ggtctgactg gtcgtaaaat
    23401 tatcgttgat acctacggcg gcatggcacg tcacggtggc ggagcattct ctggtaaaga
    23461 tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt tatgtcgcga aaaacatcgt
    23521 tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga
    23581 accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg aacaactgac
    23641 tctgctggta cgtgagttct tcgacctgcg cccatacggt ctgattcaga tgctggatct
    23701 gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc
    23761 gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt
    23821 cttcacctgc tttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg
    23881 gataggcatt cacgccgcat ccggcaaaaa gctgcccgca caataacatc attcttcctc
    23941 aacacgtttt atcgcaaatt atcatcacta ctgaaaccga ttacaccaat aacaccagcc
    24001 aaatatttgt aatattttca tattattatt cagtttttac agttgttaca tttcttttca
    24061 gtaaagtctt aattgcagat aacagcgttt aatctatgat gatataactc aattattttc
    24121 atgcacttaa accataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
    24181 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taaccatatt
    24241 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
    24301 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
    24361 tggcgcactg ccgtttattg cagatgaatt ccagattact tcgcacacgc aagaatgggt
    24421 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt
    24481 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatt ttgtttgttg ccggttcgct
    24541 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcccgcgttc tactggggct
    24601 ggcggtgggt gtggcctctt ataccgcacc gctgtacctc tctgaaattg cgccggaaaa
    24661 aattcgtggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
    24721 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
    24781 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
    24841 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
    24901 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
    24961 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
    25021 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
    25081 cttcgaactg gcgggttata ccaacactac cgagcaaatg tgggggacag tgattgtcgg
    25141 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
    25201 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
    25261 gatgcatatc ggtattcact ctccgtcagc gcagtatttc gccatcgcca tgctgctgat
    25321 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
    25381 tcagccgctg aaaggccgcg attttggcat cacctgctct accgccacca actggattgc
    25441 caacatgatc gttggcgcaa cgttcctgac catgctcaac acactgggta acgccaacac
    25501 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
    25561 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
    25621 gcgcgaaata ggcgctcacg attaatctcc ccaagcttcc tcccatcgcg gaggaagcca
    25681 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
    25741 ctatcgccat ccaacaggcc gttatgcgtc gcctgcggga aaaactcgcc caggctaacc
    25801 tgaaattggg gcgtaactac ccggagccaa aactctctta tacccagcgc ggcacctccg
    25861 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
    25921 acagtgaagc ctttattgaa gaagtggtgc cgcacgaact ggcacatttg ctggtatgga
    25981 aacatttcgg gcgcgtagca ccacatggca aagagtggaa gtggatgatg gaaaacgtgc
    26041 tgggtgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
    26101 tcccctaccg ctgcaagtgc caggagcatc agcttaccgt acgccgccat aatcgcgtag
    26161 ttcgtggcga agccgtctat cgctgtgttc actgcggcga acaactggtc gcaaaataac
    26221 catctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
    26281 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
    26341 gtggtactga gcgcagcatt ttccggcccg gcgttggccg aaggtatcaa tagtttttct
    26401 caggcgaaag ccgcggcggt aaaagtccac gctgacgcgc ccggtacgtt ttattgcgga
    26461 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
    26521 gtgcgcaaaa atgaaaaccg agccagccgc gtagagtggg aacacgtcgt tcccgcctgg
    26581 cagttcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
    26641 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggtgaggtg
    26701 aatggcgatc gcggcaactt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
    26761 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
    26821 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
    26881 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
    26941 gagcgcgatg aacgcatcgc gaaggtgcaa ggcaatcata acccgtatgt gcaacgcgct
    27001 tgccaggcgc gaaagagcta acctacacta gcgggactct ttttgttaac ccctacccca
    27061 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
    27121 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
    27181 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
    27241 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
    27301 ggaaggcaag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
    27361 gcgtggtgaa aaaatggaat ttacgatcca gaaatcgatc gaactcggtg taagcctcat
    27421 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
    27481 gctccagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
    27541 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
    27601 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgccgt taccggttga
    27661 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
    27721 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
    27781 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
    27841 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
    27901 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
    27961 gatgggcgat ctgtatctga tcaatggcga agctcgcgcc catacccgca cgctgaacgt
    28021 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
    28081 tctcgatgtg ctcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
    28141 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
    28201 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
    28261 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
    28321 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
    28381 cctcggcgtg attgccgaaa ccctgactga gcatggcact cgctactgca tggcgcaaaa
    28441 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
    28501 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
    28561 tggtcgcggt gaacctcggc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
    28621 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
    28681 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
    28741 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
    28801 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
    28861 gggcgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
    28921 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
    28981 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
    29041 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
    29101 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
    29161 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
    29221 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
    29281 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
    29341 gtggctaacg gcctcggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
    29401 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcagggca
    29461 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
    29521 gtcggccaac gcattaccgg cactgctcgc cctttgcctg caattaaagc acaggacggt
    29581 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagccgga cgaaatcatc
    29641 gtcggtttgc cgctgaatat ggacggcacc gagcaaccat tgactgccag agcgcgtaaa
    29701 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgaacgtctt
    29761 agcaccgtgg aagcccgttc cggtctgttt gaacagggcg gctatcgggc gctcaacaaa
    29821 ggcaaagtcg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    29881 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    29941 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    30001 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    30061 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttatc atccccacct
    30121 gctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    30181 ccaccgcggg agtgttaatc aataattcaa acagcgccac gcgtccttcc tgtttatcca
    30241 cttccagctt ttgtgacaac acggcccgta aactacctgc cagttgatta cgtacgggat
    30301 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgcg
    30361 tatgtaatgt tgctagcacc aaatgcccgg tttctgccgc cgtcagtgcc agacggattg
    30421 tctcgctgtc acgcagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcggccc
    30481 gcaatcccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    30541 gctggctggc atagatatat tcaacaggat cttccagcgt cagaatatgc gcatcggcat
    30601 gttgattgag atagccaacc atcgccgcca gcgtggtaga tttgccgctc cccgtcgccc
    30661 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc gttggtgcgc
    30721 caagctgttc gagctgcggg cagtgcgaag gtaacagccg taacgccagc gaaatgccat
    30781 gccgttgcgc gaatgcgctg ccacgcaatc gctggttttc cgccagcgac acggcaaaat
    30841 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    30901 gctcttcgac gtccagcgca tcaaacggcg cagcttccat tagcccgcga atgcgccatc
    30961 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    31021 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    31081 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    31141 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    31201 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    31261 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    31321 aaaagccgcc tggtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    31381 gctacccgcc tcaacgatca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    31441 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    31501 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    31561 cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    31621 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    31681 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gaactgcaat ttttggtgcg
    31741 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    31801 tttcaacggt cattgagctg tataccatgg tgctgttatt acgcatctgg atgcagtggg
    31861 ctcattgtga tttttacaac cccttctcac agtttgtagt gaaggtaacg cagccaatta
    31921 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    31981 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt taaagtggtg accttcctgc
    32041 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    32101 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    32161 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    32221 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgtc atcaatatgg
    32281 gtgtcgcaga agtattacag gcaaccggaa acatcctgct gccggggatg tggatggcgt
    32341 tatgaatgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    32401 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    32461 gccgccagtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    32521 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    32581 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    32641 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    32701 ttagcgactt tggtcttgat atcgtggcac aaacagacct cggcgttgat tccgctgaag
    32761 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    32821 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    32881 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    32941 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcacgt ttccactgcg
    33001 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    33061 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    33121 tcgtaccttc cgaagggaaa accgccgccg aactgacccg cgaagaaaag agcgccattt
    33181 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    33241 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    33301 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    33361 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    33421 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctgga cggcgtgcgt
    33481 gcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc tggtacggta
    33541 gaagccgatc gctttgtcga ttatcagcgt gctggtgtga accgcatctc tattggtgtg
    33601 cagagtttta gcgaagaaaa gctgaaacga cttgggcgca ttcatggccc gcaagaagcg
    33661 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    33721 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    33781 ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg aacccaatac gctgtttggt
    33841 tcgcgaccac cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    33901 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    33961 tatcagtgcc agcacaatct caactactgg cgatttggtg actacatcgg tattggctgc
    34021 ggcgcgcacg gcaaagtcac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    34081 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    34141 gataagccgt ttgagttctt tatgaaccgc tttcgcttgc tggaggccgc gccacgcgca
    34201 gagtttagtg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    34261 attgcccagg gttatctcac agaatgtgcg gattactggc agataacgga acatgggaag
    34321 ctgtttttaa attcgctgct ggagcttttt ctggctgagt aaacttgtat tgccggatgc
    34381 ggcgtgaacg ccttatccag cctacatgtg gcaacggttg taggtctgat aagacgcgca
    34441 agcgtcgcat cagacgttga ttgccggatg cggggtcaac gccgcatcct gccacaaatc
    34501 gtgcactata tcaaacttac ttaatcaccc cattgcgccg cagttgttcg gtatcttttt
    34561 tccagcgttc attagcttgg ttgatatcgt caattttcat caaaatcgcg acttttttac
    34621 tgtttatttc tgaaactttc atcgttaacg gatattgccc tttttcgagc gttacccagt
    34681 catcctgcaa tttacggctg agtttttgtg attgcagaaa agtctgtcgc cgttgttcgt
    34741 tgatatcgaa atctcgtttc tgctgaatcg gcacttcata acactcttca ctctgtttat
    34801 gacatgccgc aatcgccggt tgataaacct ctttgtcata tttaaccgcg aacgcctcgc
    34861 tatcgttctg tttattaaac tcatcacgct gttggtgaat ttttttaaag gcttcttcgc
    34921 gggtcatttg cttaccgttt gcatcttttc cccaatatgc atccgctgct ttctgcgccg
    34981 ccaccatttg cttatcaagc gctttcactt ccttttccag cgcaggaatc gagttcttct
    35041 gggtattcaa cgcagattca agctgactaa agcgataatt gaaatcttcg ctattcacga
    35101 tcagatattt attattcgtt ttgatatcgt cgaccacccg cccacgatcg ctggctgccg
    35161 actgaaaaga gtaaaagttc accgaccagc cagacgcggg cgttccttta ctggttaaca
    35221 tcgccgagaa ttttaccggt ttatctttcg tccaggtctg ttcgagcagt tcgtaatctg
    35281 ccaactgacc gacccaggta tagagatcgt cactggaaga gacatcgcct tccgctgacc
    35341 aggtggcctg attaccctta gcatcaagat tttttaaggt gatagcatca agcttgagaa
    35401 tgccgtgata ctgctttttg aattgatctt ttaagatatt ttcagttggc ggttcgccat
    35461 ctgcccaggc attaccagtc atcaacataa gcagtgccgt gccaacaatc cattgctttt
    35521 tcacccgaaa aacctcacgc gaaataattt attccggtga ttatcattag ggtaatgaaa
    35581 attggcaatg gacgtaagtc tgaagtgaaa aagccccggc acgataccgg agcgaggtga
    35641 ttagtactga ttgaagatct gctggatctg ctgcggatct ttggtttgcg tcagagccag
    35701 ctgcagcagg acgcgcgctt tttgcgggtt cagcgtgcca gaggcgacga agccgtattt
    35761 cgcatcatcc acttcagcat cctgagtggt agcacccgtc ggtacgcggg aagaacgcac
    35821 tactgcagtg ccgtttttcg cggcggttgc cagggtgtcg aacacggatt tatacaggtt
    35881 accattaccc acaccagcgc taacgatgcc atcatagccc gcatctacca gtgctttagc
    35941 cggaagatcg gatgcgttag cgtagttata aacgatgccg actttcggca gctcattcag
    36001 cttagagaca tcgaatggcg tatcgctggt gtgcttacgt gccggggtac gttggtagtc
    36061 aatcttaccg ttgtgaatgt atcccagagg accgtagtta acagacttga aggtcgctac
    36121 gtcggtggtg ttggttttgg ttacatcgcg accgtccagt acggtgtcgt tcatcaccac
    36181 cagcacgcca cgattagcgg atgctttatc agctgcggtc actaccgcgt tatacaggtt
    36241 gaatggaccg tctgcgctca tggacgtgga cgggcgcatt gcgccgacca tcaccaccgg
    36301 tttgtcgcat ttcaccgtca ggtcgaggaa gtaagcggtt tcttccatcg tgtcggtacc
    36361 gtgggtaatg acgaagccgt cggttttatc gcagtcggtg ttaatttttt tcgccagtgt
    36421 cagccagaca ttatcgttca tgtcctggga gccgatattc actacctgct cgcctttaac
    36481 gttcgcaatg tcttttagtt gcggcaccgc attaaccaga ttttctacgc caactttacc
    36541 cgctgtgtag ttagatttgg ttgcggagtc accaccaccg gcaatggtcc cgccggttgc
    36601 taaaatggtg atattgggta atgccaatgc tgcaccacta aaacccataa ccagtgcggc
    36661 aagtgccgtc tttttgaaaa actccatttc attcctccag ttacgtgaac gctacgcatt
    36721 atcccttagc tctgtatggg aaatttgacg ttaaacaatt tacaacgtga atatattttg
    36781 gagctctaca aagttagagg caggtaacaa aacgaagaat taaacggcat aaaaaagtat
    36841 tatgccgtct taaaatagag gattatttta aattcccgac cagggctttg cggctatctt
    36901 ccagagtcac aacgcggcta caaacatctt tgccaaactg ctggaaatct ttttcctgct
    36961 ttttccactc ggtttggatt gaggattgca gcccccccag gcttcccagc acattctgta
    37021 atgggttacc gccgcttttc agcaccgctt tcgcgcccat ttcattaatg ctgtcctgta
    37081 aaattccacc cattgcctga ttcactaatt gctggccttc ggcgcgaacc tgatcaatgg
    37141 ctttatagtg aaacgtcagg ccatcgctgc gcgtttcgat aatgcggttc atctgctctt
    37201 tcagttgcgc atcaagtttg gtcagacggc tgcgcatttt gctgctttcg cccatctcct
    37261 gaacgataat tttatccagc gcaatacggg ctttttcgac gcggcttttc gcgccttcat
    37321 caatccacgg cagcgtgctg cgtagttcag cctgataatc cttcgcctgc tcgcgctggg
    37381 cggcattcag ggaatattgc ttaccgttat acatcacgtt gccgtctggc gtgatcacca
    37441 gattgccgtt ttcgcccttc acctgcacgg tttgcgggct gacaatcaca tcgtcacgcg
    37501 gcgtgacgct gcactggtag tcggcgtgag cggtcattgc cgtcactgaa agtgctgccg
    37561 ccagcagcat tttgcgcatc atagtcttcc ctcaagaaaa aatcaggcca gcatttgctg
    37621 gccccagatt gataacaaag tacgcgtttt ccatgccgga tgcggcgtaa acaccttatc
    37681 ccgcctactc gatcgtgcat ctttccaggc ctgataagcg tagcgcatca ggcagttttg
    37741 catttgtcat cgcccgtatg ctttcttagt cccaccaaac gtcgaaaagt tcgctggtgc
    37801 gtacctcatc cagtttgcgc tcttccagcc acttacgcac aatcgcctga tgttcttcgg
    37861 tgcatttgcc gatttcctgc atgcagatca gaccttccca ggccagataa ccgctgccgt
    37921 caaaggccag tttgttcggt tcgataacct cgttaataaa atcatcaacg gttttatcaa
    37981 tctgttcttc cgatgtacct tccgggaatc gccatgccac cgaaaatcct aattcctgga
    38041 attcgtcgat gtgcattttt ttacgcagac gacggctacg gttctttgcc attatttcac
    38101 cctctcgaac attaagtccc atactccgtg accaagacga tgaccacgtt gttcaaattt
    38161 cgtcaccgga cgtgatgccg gacgcggtac gtaatcattg ctctctgaca ggtttttata
    38221 accgtcaata gaagacatca cttcaagcat atgttccgca taaggttccc agtcggtcgc
    38281 catatggaat acgcccccca gctgcagttt gctttttacc agttcggcaa acggcacctg
    38341 tacgatacgg cgtttattat ggcgcgcttt gtgccacggg tcagggaaaa agagctgcac
    38401 catgcgcaat gaattgtcag gaatcatttt atgcagcact tcaaccgcat cgtgacacat
    38461 cactcgcagg ttgtttaaac cttcttcatg cgcagaagcc aggcacgcac caacgcccgg
    38521 tgaatgcact tcaatgccga ggaagtcctg ctcagggcga tctttagcca ttgccaccag
    38581 cgacgccccc atgccaaaac caatctcaag cgtcaccggc gcttcacggc caaaaagcgc
    38641 ggggaaatcc agcatatctt cgctgaactc aacgcccatc accggccagt agttttccag
    38701 cgcatgttcc tggcctttgg tcagtcgccc ctggcggcgc acaaaactac ggatacggcg
    38761 cagtgggcgg ccgttttcat caaattccgg tgaaatgacg tcgtttttca taaagattta
    38821 gtcgcttgtg aaagtgttct gaaaacgggc attatccaaa gttagttgcc ggatgcaagc
    38881 atgataaggc cgtggctgcg gaaagttccg gtttacaccc tgccgtcgct gtgctgcaat
    38941 cttgccccca acaacagtga attcggtgac catgcaagcg tcgcaatttt cagcccaggt
    39001 tctggactgg tacgataaat acgggcgaaa aacgctgccc tggcaaattg acaagacgcc
    39061 ctacaaagta tggctctcag aagtgatgtt gcaacaaact caggttgcga ccgttatccc
    39121 ctattttgaa cgctttatgg cgcgcttccc gacggtgacc gatctcgcca atgcgccgct
    39181 cgacgaagtt ctccacttgt ggaccgggct tggctattac gcccgcgcgc gcaatctgca
    39241 taaagcggca caacaagtgg cgaccttaca cggcggtaaa ttcccggaaa cctttgaaga
    39301 agtcgcggcg ttaccgggcg tcgggcgttc caccgcaggc gcgattctct cgctttctct
    39361 gggtaagcac tttccgattc tcgacggtaa cgtcaaacgc gtgctggcgc gctgctatgc
    39421 tgtaagcggc tggcctggga aaaaagaggt cgagaataaa ttatggagtt tgagcgagca
    39481 ggtgacgccc gcggtcggcg tggaacggtt taatcaggcg atgatggatt tgggcgcgat
    39541 gatttgtacg cgctcgaagc cgaaatgttc gctctgtccg ctacaaaacg gatgtattgc
    39601 cgccgccaat aatagctggt cgctttatcc gggcaaaaaa ccgaaacaga cgctgccgga
    39661 gcgcaccggc tactttttgc tgttacagca cgaagatgaa gtattgctgg cgcagcgtcc
    39721 gccgagcgga ttgtggggcg gtttatactg tttcccgcag tttgccgacg aagaaagt
//
LOCUS       DADLTD010000011        37819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLTD010000011 DADLTD010000000
VERSION     DADLTD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.011"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..378
                     /locus_tag="JN701_000448"
     CDS             <1..378
                     /locus_tag="JN701_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7362992.1"
                     /translation="ICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEKF
                     MNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLYI
                     SYNKSSLNNLVFSRFIDRLNESF"
     gene            525..2042
                     /locus_tag="JN701_000449"
     CDS             525..2042
                     /locus_tag="JN701_000449"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY7362993.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2043..3192)
                     /gene="ybdL"
                     /locus_tag="JN701_000450"
                     /pseudo
     CDS             complement(2043..3192)
                     /gene="ybdL"
                     /locus_tag="JN701_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3301..4389
                     /gene="hcxA"
                     /locus_tag="JN701_000451"
     CDS             3301..4389
                     /gene="hcxA"
                     /locus_tag="JN701_000451"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY7362994.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4399..4596)
                     /locus_tag="JN701_000452"
     CDS             complement(4399..4596)
                     /locus_tag="JN701_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7362995.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4778..6883)
                     /gene="cstA"
                     /locus_tag="JN701_000453"
     CDS             complement(4778..6883)
                     /gene="cstA"
                     /locus_tag="JN701_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAY7362996.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7064..7477)
                     /gene="entH"
                     /locus_tag="JN701_000454"
     CDS             complement(7064..7477)
                     /gene="entH"
                     /locus_tag="JN701_000454"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY7362997.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7480..8226)
                     /gene="dhbA"
                     /locus_tag="JN701_000455"
     CDS             complement(7480..8226)
                     /gene="dhbA"
                     /locus_tag="JN701_000455"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY7362998.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8226..9083)
                     /gene="entB"
                     /locus_tag="JN701_000456"
     CDS             complement(8226..9083)
                     /gene="entB"
                     /locus_tag="JN701_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY7362999.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILLLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9097..10707)
                     /gene="entE"
                     /locus_tag="JN701_000457"
     CDS             complement(9097..10707)
                     /gene="entE"
                     /locus_tag="JN701_000457"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY7363000.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10717..11892)
                     /gene="entC"
                     /locus_tag="JN701_000458"
     CDS             complement(10717..11892)
                     /gene="entC"
                     /locus_tag="JN701_000458"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HAY7363001.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12266..13222
                     /gene="fepB"
                     /locus_tag="JN701_000459"
     CDS             12266..13222
                     /gene="fepB"
                     /locus_tag="JN701_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7363002.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13226..14476)
                     /gene="entS"
                     /locus_tag="JN701_000460"
     CDS             complement(13226..14476)
                     /gene="entS"
                     /locus_tag="JN701_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY7363003.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14587..15591
                     /gene="fepD"
                     /locus_tag="JN701_000461"
     CDS             14587..15591
                     /gene="fepD"
                     /locus_tag="JN701_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY7363004.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15588..16580
                     /gene="fepG"
                     /locus_tag="JN701_000462"
     CDS             15588..16580
                     /gene="fepG"
                     /locus_tag="JN701_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY7363005.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16577..17392
                     /gene="fepC"
                     /locus_tag="JN701_000463"
     CDS             16577..17392
                     /gene="fepC"
                     /locus_tag="JN701_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7363006.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17389..18522)
                     /gene="fepE"
                     /locus_tag="JN701_000464"
     CDS             complement(17389..18522)
                     /gene="fepE"
                     /locus_tag="JN701_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7363007.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18737..22618)
                     /gene="entF"
                     /locus_tag="JN701_000465"
     CDS             complement(18737..22618)
                     /gene="entF"
                     /locus_tag="JN701_000465"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY7363008.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22615..22833)
                     /locus_tag="JN701_000466"
     CDS             complement(22615..22833)
                     /locus_tag="JN701_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY7363009.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22836..24038)
                     /gene="fes"
                     /locus_tag="JN701_000467"
     CDS             complement(22836..24038)
                     /gene="fes"
                     /locus_tag="JN701_000467"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY7363010.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24281..26521
                     /gene="fepA"
                     /locus_tag="JN701_000468"
     CDS             24281..26521
                     /gene="fepA"
                     /locus_tag="JN701_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY7363011.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26696..27316
                     /gene="entD"
                     /locus_tag="JN701_000469"
     CDS             26696..27316
                     /gene="entD"
                     /locus_tag="JN701_000469"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY7363012.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27438..27590)
                     /locus_tag="JN701_000470"
     CDS             complement(27438..27590)
                     /locus_tag="JN701_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7363013.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28044..29162
                     /locus_tag="JN701_000471"
     CDS             28044..29162
                     /locus_tag="JN701_000471"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7363014.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29228..29476
                     /locus_tag="JN701_000472"
     CDS             29228..29476
                     /locus_tag="JN701_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY7363015.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29541..29909
                     /locus_tag="JN701_000473"
     CDS             29541..29909
                     /locus_tag="JN701_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7363016.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30003..30656
                     /gene="nfsB"
                     /locus_tag="JN701_000474"
     CDS             30003..30656
                     /gene="nfsB"
                     /locus_tag="JN701_000474"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY7363017.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30764..32011
                     /locus_tag="JN701_000475"
     CDS             30764..32011
                     /locus_tag="JN701_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY7363018.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(32079..33455)
                     /gene="pheP"
                     /locus_tag="JN701_000476"
     CDS             complement(32079..33455)
                     /gene="pheP"
                     /locus_tag="JN701_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY7363019.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33557..36700)
                     /gene="cusA"
                     /locus_tag="JN701_000477"
     CDS             complement(33557..36700)
                     /gene="cusA"
                     /locus_tag="JN701_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY7363020.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYDISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGLAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIVEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGGAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRYRVRK"
     gene            complement(36712..>37819)
                     /gene="cusB"
                     /locus_tag="JN701_000478"
     CDS             complement(36712..>37819)
                     /gene="cusB"
                     /locus_tag="JN701_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAY7363021.1"
                     /translation="RKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVR
                     IDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVG
                     DKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLI
                     ATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWL
                     VKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWL
                     QLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLAE
                     GEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         9078 a        10548 c         9631 g         8562 t
ORIGIN      
        1 atttgtagta ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca
       61 gaagagttca cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata
      121 gacgagaagt ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt
      181 aatagcattg cagttacaga tttactggga atagtcccct atgaacttta taactcccac
      241 cgtgactttc ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt
      301 tacatttcct ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt
      361 ctaaatgaga gtttttgata tataactaca gacagctact ataactttca tctatttatt
      421 cacagcgcga atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt
      481 cttatctcac tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa
      541 ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg acactcaaca
      601 ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc
      661 tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg
      721 aggcgcaatt ctcttgcact attaactatg ttcagtccct gcgcgagttg tacaccgatg
      781 tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat
      841 accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag
      901 atgcgataac cgatcctgac tttttctgct tttaccagcc aggcatgacc ttcgaacaat
      961 ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg atgatcggca
     1021 tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt
     1081 ttgccgacga taaaccatgg actacggccg caccaggcgg tcatagctgg tacatttacc
     1141 ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat caaagtctct
     1201 gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat atgcgaattt
     1261 gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt atcgaaccgg
     1321 atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc atttacgccg
     1381 gacatgacaa tcatttctat gggcatcgga aaatcctcaa gccagaacat ttagactggc
     1441 aagaatatgc attattgctg ctcaatagca tgccggaaga tgatcgcatc aaagcgatta
     1501 atgaaatccg catggcgatc catcaggtta gcccctttcg tgaagagcca gtggattgcg
     1561 ttctgtgggt taaaaacagc caacttatgc ctaatgatta caacccaaac aacgtggcac
     1621 cacccgaaaa aaaactgctg caaaaatcca ttgaaataga tggttttacc caacccattg
     1681 tggtcacaca tacggataaa aatgccatgg aaatcgtgga tggttttcac cgccatgaaa
     1741 ttggtaaagg ctcatcatca ttaaaattac gcttaaaagg ttatttgccg gtgacctgtc
     1801 tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg tcataatcgc gctcgtggac
     1861 gccatcaaat caccgccatg tcggaaatcg tccgtgaact tagccagttg ggatgggacg
     1921 ataataaaat tggcaaagag ctgggaatgg acagcgacga agtgctgcgc ctgaagcaaa
     1981 ttaacggcct gcaagagttg tttgccgacc gtcaatattc ccgcgcctgg acggttaaat
     2041 agctaaagct ggcgcaggcg ttcagctgct gccagcaacg tcgattcctt cttggcaaaa
     2101 cagagacgaa tcagtttatg tgggaaggga tcggcgcaaa acaccgacag cggaatcgcc
     2161 gccacgccgt gctcccgcgt cagccactgg caaaactcaa catcatccag ggtagaaacc
     2221 tcgctgtaat ccaccagcaa aaagtatgta ccttcacacg gtaaaatctc cagccggctt
     2281 tcatttaaag cattcaccag aatatcgcgc ttctggcgat aaaagtccgg taacgcaaga
     2341 taatgctcag gttctgcacg tagcatatca gcaagcgcca gctgtgccgg ggtattcacc
     2401 gaaaaggtca gatactgatg taccttgcga atttcggcgc tgatgggcgc tggcgcaaca
     2461 caataaccca ctttccagcc ggtcatatga tagatcttgc caaatgaaga aaccgccact
     2521 gcccgctcac gcagctgcgg atgcgccagc acactggcat ggccctgttg tgaaaagttg
     2581 atgtgctcgt agacttcatc gctaatgaca aaaatctcgt gcccggcgat cgcctgccac
     2641 aaagcggcga aatcagcctg ctgccagaca gttgcactgg ggttatgcgg agtgttgagg
     2701 atcaccagtc tggtgcgctc gcttaataat gcggcaaatt cctgccagtc aaggaggttg
     2761 cagtgccata cgcttcacta ttcccccaga aagcgcgatg gcgggggcgt aactgtcata
     2821 gctgggatca aaacaaatca cttcatcgcc attgcgcacc agtgcggtaa tcgccgcgta
     2881 taacgcttcc gtcgcccctg ccgttacggt gatatcgcta tcggcatctg gttgatagcc
     2941 atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag gcctgcacgc cggtcatggg
     3001 cgcgtattgg tttgccccct gggcaacgtg gtacgccagc cgctcctgta aatagcgcgg
     3061 accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc tggtgttgct gcgccagcgc
     3121 gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt ttgctttgtg gaatcagagg
     3181 gttatttgtc atttttattg tacctgacga agttgcgtgt tgaaggcact ataacacgat
     3241 gctagtattt ggcaatcaag acgtttagat gtctaaataa aacaataagg acaacacaac
     3301 atgcctcaca atcctatccg cgtggtcgtc ggcccggcta actacttttc acatccaaga
     3361 agtttcaatc acctgaacga ttttttcact gatgaacaac tttctcgcgc ggtgtggatc
     3421 tacggcaaac gcgccattgc tgcggcgcaa accaaacttc cgccagcgtt tggacttcca
     3481 ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa gcgatgtaca gcaactggcg
     3541 gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg gcggcggcgc actgctcgac
     3601 accgcgaaag ccctcgcccg ccgtctcggt ctgccatttg ttgccgttcc gacgatcgcc
     3661 gctacctgcg ccgcctggac accgctctcc gtctggtata acgatgccgg acaggcgctg
     3721 cattatgaga ttttcgacga cgccaatttt atggtgctgg tggaaccgga gattatcctc
     3781 aatgcaccgc aacaatatct gctggcgggg atcggtgaca cgctggcgaa atggtatgaa
     3841 gcggtggtgc tggctccgca accagaaacg ttgccgctaa ccgtgcggct ggggatcaat
     3901 aatgcgcagg ccattcgcga cgtcttgtta aacagtagcg aacaggcact tgccgatcag
     3961 caaaatcagc agttaacgca atcattttgc gatgtggtgg atgctattat tgctggtggt
     4021 gggatggtcg gtggtctggg cgatcgtttt acgcgtgtgg cggcggctca tgccgtgcat
     4081 aacggtctga ctgtcctgcc gcaaaccgag aagtttcttc acggcaccaa agtcgcttac
     4141 ggaattctgg tgcaaagcgc cttgctgggt caggatgatg tgctggcgca attaaccggg
     4201 gcgtatcagc gttttcatct gccgactacg ctggcggagc tggaagtgga tatcaataat
     4261 caggcggaga tcgacaaagt gattgcccac accttgcgcc cggtggagtc cattcattac
     4321 ctgccagtca cgctgacacc agatacgttg cgtgcagcgt tcgaaaaagt ggaatcgttt
     4381 aaagcctgac gtgcaaattt agcagcagcg cgcgccgcct tttccaccgt agcgcgcgtc
     4441 ctgccgctca cggaaaaact cctcgtaggt catcggcgtt tgatcgggat ggttaacccg
     4501 catatgttcg acatagttgt cgtaatcagg cataccaatc atcagcttcg ccgcctgacc
     4561 taaatatttt ccggcttttg ccagtgaatc aaacatcttg gcctccgcta accatgttgc
     4621 tgaaagagtg ccggatgcgg cgtaaacgct ttatccggcc tacaaatgct atcaacgcac
     4681 tcggtcagtc ccctcgcccc atcggggaga gggttagggt gagggggaac cctcggcact
     4741 atgccaacat tcgccaacat cccccctcac tctgacttta gtgcgcgcct ttcgcctgcg
     4801 ctacgatctc ctcgacattt tccggcatcg gctcatacgg cgtttctttc gccgttggct
     4861 tcggatcttt caatgccgca agtgccgtct taatcgagaa cagtgccaga accaccacga
     4921 ccaccataaa gaagatggtt aacccggcat ccagacggtt gttgaacacc agttgcgcca
     4981 gctgtgactc agtatactgc gacggaatat tgccgctgtc gatcattgcc tggaacttgt
     5041 tagcaatggc caggaagccg actttcgcat ccgggctaaa cgctttctgc cagccagcgg
     5101 tcagggtaca aatcagcagc caggccgttg gtaccagcgc cacccaggcg taacgttgac
     5161 gtttcatctt gaacaacacc acggcacaga gcatcagcgc catccctgcc agcatctggt
     5221 tggcaatacc aaacagcggc cacagagtgt taataccgcc cagcggatcg accacgccct
     5281 gatggaggaa gtagccccac gccagtacgc acagcgccgt tgccagcagg ttagcaggca
     5341 gtgaatcggt ccgcttcagg ccaggagaca ccacgcccag cagatcctgc aacataaagc
     5401 gcgcagcacg cgtacccgca tctaccgccg tcagaataaa cagagcttca aacagaatgg
     5461 cgaagtgata ccagaacgcc acatccatca taccacccag cgcaccgtgc aggatgtaag
     5521 ccatccccac cgccaacgtc ggcgcaccgc ccgcacggga aatgatcgac tgttcaccca
     5581 cttcgctggc aatctggttg agcgtatctg gtgtaatgct aaagccccag ctactcacca
     5641 cctgcgcggc agaagcgacc acatccgccg tccctgctgg agccagcacc gccattgggc
     5701 tgttcatggc aaagtaaacg cccggatcga tgatacaggc ggaaaccagc gccataatcg
     5761 ccacgaagga ttccattaac attccaccgt agccgataaa acaagcctgc ccttcgttcg
     5821 ccagcatctt cggcgtggtg ccggaagaga tcagcgcatg gaagccagac accgcgccac
     5881 aggcgatggt gataaacagg aacgggaaca ggttaccggt ccataccggg ccagtgccat
     5941 cgacaaattt ggtcagcgca ggcatggtca gcgtcgggcg cataatcaaa atgcctaccg
     6001 ccagaccgac gatcgtcccg attttcagga aggtagagag gtagtcacgc ggggccagca
     6061 gcaaccacac cggcagcacc gccgccacaa aaccgtaacc caccagcatc caggtcagct
     6121 gcacgccggt aaagtcaaag tacggtgccc aggtcggact ttctgccacc cagccgccag
     6181 agataatggc gaaaatcagg aataccagac cgatgaccga cacttcacca atacgccccg
     6241 gacgcagata gcgcaggtag atccccataa acagcgccag cggaatggtg aacgcaacgg
     6301 tatacgttcc ccacgggcta tgagtcaggg ctttcaccac gatcatcgcc agtactgcaa
     6361 ggataatgac catgatcata aagcaggcca ccagcgctat caccccggcg gttggcccca
     6421 tctcttcttt gaccagctca cccagcgatc gaccgtcacg gcgcgtagaa acaaacagca
     6481 ccatgaaatc ctgcaccgca ccggcgagaa ccaccccggc gagtagccag atcatccccg
     6541 gcaggtagcc catttgcgct gccagcaccg gcccaaccaa cggacctgct ccggcgatgg
     6601 ccgcaaaatg gtgaccgaac agcactttct tgtccgtcgg cacatagtcc agcccgtcgt
     6661 tatggcgcac cgctggcgtc atacgcttcg ggtcaaccgc cagcacattt ttggcgatat
     6721 acaacccata aaaacggtaa gcgatcagat agatacagac cgacgccacc acaatccaca
     6781 gcgcgttgat ctgttcccca cgatttaaag caatgtatcc cagagcaaat gctcccatta
     6841 cagagagcac tgtccagacg aggtatttcc ctgatttgtt catagttgtt atccgtgtgc
     6901 gtgtccatag agatgttaca ttttgtttct ataacatctt tgtacgattt aacaacacgg
     6961 taactatgta aatcggagtt gaaaccaata tttaacttaa caatgttaac tcgatcactc
     7021 cgttaaccga gttttcctgc aatctcattt aattctgtcg ggctcatccc aaaactgccg
     7081 tacccagccg acaagtgcag caacgccgcc cctgttcatc gaaaacgacg atttcccagc
     7141 tctgattttg ccgcccaaga tgcagcggct ggcagacgcc gcgtaccttg ccttcagaca
     7201 ccgggcgatg atgggtggcg ttaagttccg tgcccaccac acactgcccg tcacgggtca
     7261 tcataaatcc ggccatcgat cccagcgttt cagccagcgc cgccgacgcg ccgccatgca
     7321 gcaggccaaa cggctgatga gtacgggtat caaccggcat ttcggcttcc agcacatcat
     7381 ctcccagacg ggtatacaca attcccagat gcgccaccat tgtgttatcg ctggtggcgt
     7441 tcagttcgtc gagcgttaaa tggcgtttcc agatcatgct tatgccccca gcgttgagcc
     7501 gccatcgacc acaatatcct gtagggtgat atggctggcg aggtcagagg cgaggaacaa
     7561 aatcgtgttg gcgatctctt gtggacgggc gattttcccc agcggaatgc cgagtttaaa
     7621 ctgctcgcca aagccgcgaa tacgctgttc ttcggcgtca tcgctcaccc acagcgtgcg
     7681 ttgcatatcg gtgtcggtgg agccaggcga aaccacatta cagcgcacgc cgctacccgc
     7741 cagttccagc ccgacgctca acgccaggct tttcagcgcc gctttcgatg cgccataagc
     7801 actcatacca atacgcggcg tgtgtgcggc gtcggacgcc acagtgacaa tcgccccgcc
     7861 ccgctgacgg cgaaactggt tcatggtttg ctggaacagg ttaaacgcac cgccgacgtt
     7921 aaccgcaaaa gtctgctgcc agtcctcttt actgagctga tcggtcgcgc ccatgcgcaa
     7981 aattcccgcc gcattgatca gcacgtccag tcgctccgtt tcagctaaca gtcgctgaca
     8041 tacctgcgcc acctgcccgg cgtcggcaac atccattact tcggtcgcaa agggatattg
     8101 ctcctgagtg aacgcttgat caaaacctgt aactttcgct cccgcctcaa caaacgccag
     8161 cgccgtggcg tagccgatac ctttacctgc gccggttacc cagacatttt taccgctgaa
     8221 atccattatt taacctcgcg ggagagtagc ttccaccagg cgtcgatggt cgggtttttc
     8281 gccagcatga caaagtcgat atcaccatgc actttgcgcc agcgcgccgc cagcgccatc
     8341 atgcgcaccg aatccagacc gtagtcgatc aggttgtcgt catcgaacgg ttcatcggac
     8401 tcgtccagca acagcaggat aacctcacgc agcgccgctt tgctggcggg gacaggtgct
     8461 ggcagtaatt cttcagtcat caccacccgg ccagaccgcc cggccacata tttcagcgac
     8521 atcaaatgct cgtcacggct gaaatcggcc agcgcatccg ccaccataaa cggtttaata
     8581 tcgcgcataa atgcgtcggt tgcggtggtc atacagccaa tgtgggcata taccccggta
     8641 ataatcagct ggttacgtcc actctctttg agcatttgct ccagcggaga acgatgaaac
     8701 gcgctgtagc gccacttcac cagcaccgtg tcgtcggcat ctggcgtcag gcgatccacc
     8761 accttttgct gttccggcga acgggtcagg cccggccccc acatatcatt caacagcgcc
     8821 cgatcttcat cgctctgctc tttcggctgg gcggtgtaat aaactgggat attgtgctgt
     8881 ttgcagtagt cgcgcagcgc agcaatattc gcgatcacct gctccatcat cgggcagttc
     8941 tcgccccaga agctgacaaa atagtcctgc atatcatgga ttaacaacgc ggcacgttgc
     9001 agttcaaagg cccagtcaac tttattctgc ggaatatcgt gagactccgg cagtgcgtaa
     9061 gcctgtaatt ttggaatagc catcgtgttc tctccttcag gctgatgcgc gtgacgccag
     9121 ccactgactt aattgttttt tatcgacttt cccgaccgcc gtcagcggaa gtgaatctac
     9181 acactccacg cgatccggta atttaaattc ggcaataccc tgttcacgca ggaaacgacg
     9241 cacctgcacc gcgcgcagcg gctcttttac caccagataa gcacagcttt tttcgcccat
     9301 cagctcatct tccatgctca ccagtgcggc atagatcacc gccggatggc gcagcagcag
     9361 gttttcgatc tcttcggcag cgatcttctc gccgccacgg ttgatctgat ctttctcgcg
     9421 cccctgcacg gtgatgtaac cctctggatc aatagagatc agatcgccgg aacagtaaaa
     9481 accgttggca tcaaaggcgc tggcattgtg ctgcggactt ttgtaataac cgcggaaggt
     9541 gtacggcccg cgcgtcatca ggcgtccgac ttccccttgc ggcagtggat ttccttcggc
     9601 atcggcaacc cacacttcat cgtccggaca cattgggtaa ccctgggtat ggataatttt
     9661 ctccgcgcta tcatcaagac gggtgtagtt caccagccct tccgccatgc caaacacctg
     9721 ctgcaactgg cagccaatct cagcgggaat acgcgccgca agggtggcag aaagacgtgc
     9781 gccgccgacc tgtaacagtt tcagcgaggc aagctgcgcc cggctttcgc cttcagccag
     9841 cgcctgcaac cacaggctga ctgccggcgg caccagcgcg gtgacgttca cctgatgttt
     9901 ttcaatcaat gggaagcaaa gcgtagcgct gggatcggcc gccagaacaa ccgttcctcc
     9961 ggcaagaaag acgcccagcg atcctggcga actcatggcg tagttatgag ccgccgggat
    10021 cgcgcacagg tagcgcgtct gttgtgtgaa ctgacaaatc tcgacgctac gacgcacgct
    10081 gtagtagtag tcgttatgag tgcgcgggat cagcttcggt gtaccggtgg tgccgccgga
    10141 aagctggaaa taggccactt catcagcagg tgatggcgtg gcagtaaaac cgtctgccgg
    10201 atggttaatc gcatcctgca agttatgctc accgctgtcg ttgtgcagtt gcaccacgcg
    10261 aatggaggaa tgttctgtga cgaaagtatt gaggaaatca tccccgctaa acagcgcatg
    10321 ttggcgatcg gcaatcagca atgcgggttc aatctggctg gcataggcgt tcagttcact
    10381 acgctgatgg ctgaacaacg ccagcaccgg cgcaacgccc agtttcagca gcgcgaaaaa
    10441 ggtaatatac aattcagcga cgttacccag ttgtaccagc gcggtttcac caggtttaat
    10501 gccctgacgg cgtaaagtac acgcgaggtt atccgccgcc tgattcagct cccggtaact
    10561 caactgtagc tcgccgtcga taaccgcgat gctgtcactc gcagcgtggc gagtcagaat
    10621 gtcggtcagc ggcaaatcct gccagtagcc tttttcccga tagcgacggg caaactcttc
    10681 cggccagcgg gtgaatggaa tgctcatcct cgctccttaa tgcaatccaa aaacattcaa
    10741 catggtagaa agtttgacgc ctgtttcgcg ccactcaccc aacggtgacg acgcaagcac
    10801 aatccccgct ccggcaaaca gacgcacctg attttcccgc agcttcgcgc agcggatggt
    10861 caccacccat tcgccgttac cttcgctgtc acaccaaccc acaatgccgc caaacagttc
    10921 gcggtcgaac ggctccagtt cagcaataac ctgagtcgcg gcctgatgcg ggaaaccgct
    10981 cagcgcgggg gtcggatgca gcagacaggc cagagtcagt gcgttttctt gcgaattcgc
    11041 tttaccttca aagggagttg cgagatgcca cagcgtcggc gtggtaatca attgtggaga
    11101 ggagggaacg tgtaactcac tactgcgttc gcgcagtacc tctttcatcg cctgagtcac
    11161 cagttcatgt tcatggcgat ctttttctga cgccagcaga cgattacccg cttcgcgatc
    11221 gagcacttca tccggctgac gacgcgcgga accggctaac ggaatggagc taaaacgctc
    11281 gccgtcttta cgtagcagca gttccgggct ggcccccagc aggacgccac catcagccag
    11341 cgggacatgg aagttgtaac taaccgggtt ttgcgcaatc aaccgttcca gcaatacgcc
    11401 actatcaatg gcggcgtcag tggtgatatc aatcaaccgt gacaacacca ctttgtcgac
    11461 ctgcggcgtg gcggtaagtg cggcagcgcg ggcaaccatc tgttcaaacg tggtttgttc
    11521 cggaattgcc tggcgttcca ccacattcag cgactggctg cgggtgaaac ggcgggcgga
    11581 agcttgtttt tcctgacggg agaacgactg ccaggattca ggaatataca gcgacgaagg
    11641 ctgacgtgga tcgaagggaa tcgccccgac catcaccgga tttttgatgc cctgcacttt
    11701 ggcatcggca aacagcgcgg cgagtttttg ctggaaggga ctgtcgggcg aatccccgtt
    11761 cacagccggt tcatcgaagc gggcgaaaca tcctgacgtc gtaaaactgc ggtacggcga
    11821 cataaagaaa aagcgattgg gcgcaagtgt tgccatggtc tgctgtactt cctcagccag
    11881 tgacgtatcc atatcatcct ccacaaaatg ataaaggctt taataatgat tatcatttat
    11941 attttcggtc gctaacctaa aagcaaacgc gcactatgtc aactcttgag gtaacgcaat
    12001 ctgcaactcg gggttgcatc tggttgtggt tacaatgaaa atgagaagca ttattgatgg
    12061 attcgcataa gcgcaatgtg atgtcctgcg ccgttcttcc ccctctccct tccagggaga
    12121 gggctgggtg agggtcaata ttcgcaccag tgctggctgc tcccatcacc ctaaccctct
    12181 ccccaaaggg gcgagggtac ggatagtgcg ctttgtcgaa tttgtcatta cgcccttaac
    12241 cttattaata acaggaagct gatttgtgag actcgccccg ctctaccgca acgcccttct
    12301 attaacagga cttttgcttt ccggaatagc cgcagttcag gccgccgact ggccgcgtca
    12361 gattactgac agccggggca ctcataccct ggaaagccag ccgcagcgta ttgtttccac
    12421 cagcgtcacc ctgaccggct cactgctggc gattgatgct ccggtgattg ccagcggcgc
    12481 gaccacgcca aataaccgcg tcgcggatga ccagggcttt ttacgccagt ggagcaaggt
    12541 ggcgaaagaa cgcaaactac aacggctcta tatcggcgaa ccgagcgccg aagccgttgc
    12601 cgcgcaaatg ccggatctga ttttaattag cgcaaccggc ggggattcgg cgctggcgct
    12661 gtatgatcag ctttccacca tcgccccgac attaatcatc aattacgacg acaaaagctg
    12721 gcagtcgctg ttaacgcagc ttggcgaaat taccgggcat gagaaacaag cggcagagcg
    12781 gattgcacag tttgataagc aactggcagc ggcgaaagag caaatcaaat taccgccgca
    12841 gccggtcact gctattgtct ataccgccgc tgcacacagt gccaatctct ggacgccgga
    12901 atcagcacaa gggcagatgc tggaacaact cggctttacg ctggcgaaat tgcccacagg
    12961 gttaaacgcc agccaaagcc agggcaaacg ccatgacatc attcagctta gtggagaaaa
    13021 tctggctgca gggttaaatg gcgagtcact attcctgttc gccggtgatc agaaagacgc
    13081 cgatgcgatt tatgccaatc cactgctcgc gcacctgccc gcagtacaaa acaagcaggt
    13141 ttatgcgctg ggaaccgaga cgttccgcct ggattactac agcgccatgc aagtgctgga
    13201 acgacttaag gcgctgtttt aagcattaac tgtcggacgc tgtcacctgc ggcggcgtct
    13261 ggcgaaaacg tcgcaactcc accagcacca gcaataacaa cacgccgata atcaacaaac
    13321 caaaaccgct cgcgcttgcg gaagcaaccg gtgtcatcat cgctcccaga ccgcctaaca
    13381 gcgccgcgcc tatggcatcg cccgtcacgt tttgtgccgt ccacaaaccg ttgattcgcc
    13441 ctaacattgc ttccggcgtt tgcgtttgca gcattgtata ttgcagcaac gaactgaccg
    13501 cactcagcca gccgaacagc gccagacaaa ccacgcctaa aatccacatc ggcatcaggc
    13561 caaataaacc aatggcgagg aacgatccca acgtggagag caacatcaac aatcctggtc
    13621 gcgcgctatg cgccagtttc ccactggtta atgcgccaat agccgcaccg agcgggatcg
    13681 ccgcatagag aaaaccaatc tctgccgccg acatctgcca gttgtcagcc agcgccggat
    13741 acagtacccg caccgcgctc gccatcgtca ataaaccacc cagcaacgca atcccgccca
    13801 ccagcgggct ggcgagcaga aaacgaaatc ctgccagtaa tgatttcaac ggatgctcgc
    13861 gcggctgcgg tggcggtggc aacgccggaa ggcttaacaa cggtagcaag gtaataaacg
    13921 tgcccgccgc cgccagcccg tagttccagg ctacgccacc ggtcgccagc aataaaccgc
    13981 caatcatggg cgaaatcacc gaccccagac gcacagtcaa catggtgatc gccccggcct
    14041 gcattaagtt ttcacgccct accagtgctg gtgtcgccgc caatagcgcc gtaacgccaa
    14101 gcgatgcgaa aaaaccatcc cataaaccaa gtaaatagat tgccagcaat gacggctccg
    14161 gcagcagtgc attaaggcac agtccaatga agccaatgcc acaggtgccg cgcgccagca
    14221 aaatcacttt tttgcgctca tagcgatccg ccagcacacc gccaaccatc aggccaacaa
    14281 acatcgcgcc gccggtcagc gtcaccgaaa gccccaccag ccaggtagaa tgcgtcatca
    14341 tctggatctg caccggcacc gcgacgccga gcaaacccag agacacaatt gagataaaac
    14401 gagcgaggaa tactgcgcga aacgccgggt gcgttttcaa caggctgagg ttaagcagcc
    14461 aggattgttt attcattaca atgccttgcc atcaaatatt ttaaatacct attcttaggc
    14521 tgcacatgct aacatatcca aataagatcg ataacgataa ttaatttcat tatcatggaa
    14581 gttcgtatgt ctggttctgt tgccgtgaca cgcgccattg ccgtgcccgg attgctgtta
    14641 ttactgatta tcgcgacggc attaagcctg ctcattgggg caaaatcact ccccgcttcc
    14701 gtagtgctgg aggccttctc cggcacctgc cagagcgccg actgcaccat cgtgctcgac
    14761 gcgcgactgc cgcgcaccct tgccggttta ctggcaggcg gcgcgcttgg ccttgccggg
    14821 gcgttgatgc aaaccctcac ccgaaatcca cttgccgacc ccggcttgct tggcgtgaac
    14881 gccggagcca gctttgccat tgtgctgggt gcggcgctgt ttggttactc atccgcgcag
    14941 gaacaactgg cgatggcctt cgccggggcg ctggtggcct cattgattgt tgcctttacc
    15001 ggcagtcagg gcggcgggca gttaagtccg gtgcgtttaa ccctggcggg cgtggcgctg
    15061 gcggcagtgc tggaagggct gaccagcggc atcgccctgc ttaatcctga cgtctacgat
    15121 caactgcgtt tctggcaagc cggttcgctg gatattcgca atctacatac cttaaaagtg
    15181 gtgctgatcc cggtgctgat cgccggagca accgcgctat tactgagccg cgcgctgaac
    15241 agtttaagcc tcggcagcga caccgcgacg gcgctgggca gtcgcgtggc gcgcacacag
    15301 ttaattggtc tgctggcgat taccgtgctt tgtggtagtg cgacagcggt agttggcccg
    15361 attgccttta ttggcctgat gatgccgcat atggcgcgtt ggctggtggg tgccgatcat
    15421 cgctggtcgc tgcccgtaac gctgcttgct acccctgccc tgctgctgtt tgccgatatc
    15481 atcgggcggg tgattgtgcc cggtgaactg cgcgtttctg tggtcagtgc ctttattggt
    15541 gcaccggtgc tgatcttcct cgtgcgacgt aaaacgcgag gtggtgcatg atttacgtct
    15601 ctcgccgatt actcatcacc tgtttgctgc ttatttccgc ctgtgtggtt gcaggtatct
    15661 ggggattacg cagcggtgcc gtcacgctgg aaacctcgca ggtattcgcc gcgctgatgg
    15721 gcgatgcgcc gcgcagtatg acgatggtgg tcaccgaatg gcgtttacca cgcgtgctga
    15781 tggcgctgtt gattggcgcg gcactgggcg tcagcggtgc gatttttcag tcgctgatgc
    15841 gtaacccgct cggcagccct gacgtaatgg gctttaacac cggggcgtgg agcggcgtgc
    15901 tggtggcgat ggtgctgttt ggtcaggacc tgacggctat cgcgctggca gcaatggtgg
    15961 gcggcattgt cacttctctg ttggtctggc tgctcgcctg gcgtaacggt atcgacacct
    16021 ttcggttgat tattatcggc atcggcgttc gcgccatgct ggtggccttt aatacttggc
    16081 tgttgctgaa agcgtcttta gaaacggcgc taacagcggg tttgtggaat gccggatcgc
    16141 tcaatggcct gacgtgggca aaaacttcgc cttccgcacc catcattata ttgatgctca
    16201 ttgccgccgc cttgctggtg cggcgaatgc gcttgctgga gatgggcgat gataccgcct
    16261 gcgcgctggg cgtcagcgtc gagcgttcgc gtctgttaat gatgctggtt gcagtggtgc
    16321 ttaccgctgc ggcaacggcg cttgccgggc cgatttcctt tattgcttta gtcgcgccgc
    16381 acattgcccg acgcattagc ggcaccgctc gctgggggct gacccaggcg gcgctgtgcg
    16441 gagcgctgtt actgctggcg gccgatctct gcgcccaaca actgtttatg ccgtatcaac
    16501 ttccggttgg cgtcgttacc gtcagcctcg gcggtattta ccttatcgtc ttgttaattc
    16561 aggagtctcg caaaaaatga ccgaatcagt agcccgtttg cgcggcgaac agttaaccct
    16621 gggatatggc aaatataccg ttgcggaaaa tctgactgta gaaattcctg atggtcactt
    16681 cacggcaatt atcggaccaa atggctgtgg taaatccacg ttactgcgta ccttaagccg
    16741 cctgatgacg cctgctcacg ggcatgtctg gctggatggc gagcacatta aacattacgc
    16801 cagtaaagaa gttgcacgcc ggattggtct gttggcgcaa aatgctacca cgccgggcga
    16861 tatcaccgtg caggagctgg tggcgcgtgg acgttatccg catcaaccgc tgtttaccca
    16921 ctggcgcaaa gaggatgaag aagcggtaac gaaagcgatg caggccacgg gaataactca
    16981 tctggcagat caaagcgtgg ataccctttc tggcggacaa cgccagcgag cgtggatcgc
    17041 gatggtgctg gcccaggaaa cggcaattat gctgctcgac gaacccacga cctggctgga
    17101 tatcagtcat cagattgatc tactggaact gttaagcgaa ctgaaccgcg agaaaggcta
    17161 taccctggcg gcggtgctgc acgatcttaa tcaggcctgt cgttacgcca gccatttgat
    17221 tgcattgcgg gaagggaaaa ttgttgctca gggagcgccg aaggagattg tcactgctga
    17281 actgattgag cgcatttatg gtctgcgatg catgatcatt gacgatccgg tagccggaac
    17341 gccgcttgtg gtgccgctcg aacgaacggc accttcaacc gcaaatagtt aaactaagtg
    17401 gtctgccatc atggcatcct gttttctgga tgccatcgca tagcgcaata acacgctacc
    17461 acaagccacc atcccgccga tcagcgcgga aaggatcaca ataatcgcct tacccggacc
    17521 gtcttttttc actggcaatg acgggcttaa ctgatattta aacggcgtaa aattcacatc
    17581 gttgacattt gcttttgtta attgctcgac aagatactgc cgattacgta attcaccgtt
    17641 cagttccgca acgtcagtaa ccgctttttc aatttccagt ttgcgttcaa taccgtctgc
    17701 gccgagagaa atagagaaat cggggtcatc tttaacggcc tgaccattac tgtatacggg
    17761 ctttttaatt cctgccgcgt tggcaatgtc gagtgaataa ttgaggcgct gaatgtttgc
    17821 atcaagttga tttttcgttt taatgcgatc ctgagccagt ttttcttttt caaactgggt
    17881 tttgatctcc agtttattac ggacgttttc tatcgactct ttcaccacca acgcagagat
    17941 ataatcgata tacccgctca aaacggtctg cgcctcttta ctggttggcg cggtaaaact
    18001 tagcgtccag gaggtataca gtgacggttc atcttttttc ttactggcat tgtcatcaac
    18061 cgctttcatt ttttcgctca atgcaacaat agcgcgatgc aaatccagtt catcgatttt
    18121 cgcctctttt aattggtcca tcacataagg tgatgaacgc aggtactctt ccagcaagct
    18181 aacagactga aacttcttga taaacaggtt aaatgcaccg ccacggtcga tattaacatc
    18241 gagatcaaga acgcggagtt tggtaaaggt tttctccagg tcctgccact gaatggcttc
    18301 ggcgggagta ataacggctg agctggtcca tttttgcggc aggatgaaag agatcagtaa
    18361 acctgcgcag gcaaatgcaa aaacgaccgc catgaccgtt tttttggccc gccataaaac
    18421 ttcgattaga ttaagtaaat caatttcatt actactgggc gacgccagag gatactcgga
    18481 aaaatgagcg tcacttccct gtttaatatt cagtgatgac atgggtataa cctgaaaatg
    18541 agtccaatag ggggaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    18601 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    18661 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta attatgggtt
    18721 tatagataat attaatttac ctgtttagcg ttgcgcgaat aatcggccca atttttacaa
    18781 acgcccctgg agagataata tccacatgcg cacaatcctg acgatagata tccagctcgg
    18841 caatccaggg cgaccaagcg cgttcggggc tcataccttc ctgaagcgtg cgttcagcaa
    18901 caaacagcgt cgctttacca tcaaacggta cgctatgagc agtcgtcagc agacgcacag
    18961 catcagcgta gttgccttca atggtggtaa acaactccgt tgaagtactt ccctgctgtg
    19021 ccgccaggaa ggcctcgcgt tcgcggttaa tctccgccag cacttccggg tccagaccat
    19081 tagcctcttt ttcctgccag ttttgcgttt ctggcggcca ggtatccagc aaaccaagaa
    19141 atgccacctg ttcgccacgg gcacgcagtc gcgccgcaat gccctgcgcc agcgtaccgc
    19201 caagggaata ccccagcagg taataagggc cgtgcggttg ttgttcaagt aacgttgcca
    19261 gatgcgcttc acagacttca tccagatttg ccgcggtatg catggggcca ttggggcgcg
    19321 gcgactgaat gccgataatc gaccattgtg gatcgatata acgcgagagc acgctaaact
    19381 gccaggcaaa accggatgcc ggatggaaac aaaacagcgt cgggccatta ccttcacgca
    19441 acggcagaat ggtttcgaat cccatacgcc gggtgctgtc ttcttcagca tcaataatcg
    19501 ttgccagttt agcgacggtt gacgcgacca tcacttgccc cggcgtcacc tggcgggcaa
    19561 cctgccgact taactgcgct gccagtttca ttgccagtag cgaatgacca ccaagcgcaa
    19621 agaaatcagc atcggcatcc tgcacgtcac aacccagcaa cgacgcgaat gccgcggcaa
    19681 taatcgtttc actgcccgct tttggcgcac gccccggcgc ttgtgccttc agttcaggca
    19741 acggtaaggc tttgcgatcc agcttgccgt tggcgctaag aggtaacagt ggaagttgca
    19801 gcagaaccac gggcaccata tgtggtggca atgtttcgcg aagctgcgcc tgtaatgcgc
    19861 tggtatccaa cggcagacct gattgcgaca ccagatagcc caccaactga cgcgcatcac
    19921 caccggtggc tgccgcctgg ttaatcacac aggcgtgggt aacggcttgt tcgacatccg
    19981 gcagcgcctg catcacgcga tcgatttcgc ccagttcaat acgctgcccg cgaattttta
    20041 gctgatcgtc actgcgcccg aggtactcca ccgcgccgtt atccagccag cgggcaacgt
    20101 ctccggtacg gtacatccgt tcaccaggga caaaaggatc ggcaataaag cggctggcgg
    20161 tcagatcggg tcgtccaaga tacccctgcg ccagttgaat gccggtgaga tagagatcac
    20221 ccgccacacc cggcggcacc ggatgcatca tcgcatcaag aatacgcaga cccgtattcc
    20281 ataccggata accaatcggc acactgctgc cgcgcacctg tgccagttcc tcgccaaaag
    20341 ccggatacca gctgacatct accgccgctt ccgtcgggcc atatagatta tgcaacggca
    20401 cgctggttaa ctgttgccat tcgcggcata aatcagccgg taaggcctca ccactacaga
    20461 aaacctgttt caacgtcgcg caactctggc gagcggtttg cggcgtcagc gatgcaacaa
    20521 atgctgccag catcgacggc acaaagtgcg tggtcgttac gccatattcg gcaaagaatt
    20581 gctgcatagc gagcgggtcg cggtgcgctt ccggttcagc catcaccagt ttagccccgg
    20641 caataaacgg ccagaaaaac tcccacaccg agacatcaaa actgcacggc gttttttggg
    20701 caacgacatc ttcgcctgta agcagataat gattttgcat ccacaacagg cggttaacga
    20761 tagccgtctg cccgaccatt acccctttcg gcctgccggt ggagccagag gtaaagatga
    20821 tataagccgt gtgatgcggt tgtgaaagtt gcagcggcgc actgccctgc ggtttaagcg
    20881 gggcgttata gcaaaggttg gttaaatctg gaacatcggc aaagcgcggc agttgatcgt
    20941 cggtggtgat taacagcgac ggacgcgcat cttccagcat cattttcagg cgatcgtccg
    21001 gatagccggt atccagcggt agccaggccg cgccagcttc aactatcgca tgtagcgcca
    21061 gggtcaaaaa gaccgagcgc ggtagcgcca ccgccacgct gtcgcccggt ttaacgccac
    21121 gctcacgcag cagattcgcc agcgccacca cctgctcgcg catttcccga tagctgaact
    21181 ggtaacgcgc atctgccagc gccggagcat ccggtgtttt tgccgcttgt tctgccacca
    21241 gcgcgctcag cgtggtttct ggaatctcaa cctgagtggc gttgatctgc gccagctgcg
    21301 catactcacc tggcagcata atatcgacat cgccgcacaa cagcgccgga tccgcggcga
    21361 actgggcaat cagcattttc aggcgttcag catgctggat taacgttggc tcatcgtaac
    21421 gctgtttatt ggcgaggatc tcaatactca aatcaccgtg ttcatccggg aacagggcca
    21481 gttcaaggtc attaaccgga ccggttgcca aggtatgggt ttgcgcctga acaccaggaa
    21541 tatccagttg gtaatcaaat accttgatat tgagtaccgg accaaacagc ggttcatcac
    21601 ctgccgctcg cccgctgtca cggacaattt gttcggcatc gtaacgttga tgacgacgca
    21661 tttttttcag ttgtgctgcc agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa
    21721 tgtgaatacc caacggcaaa acgttgagca cgggtccggt agccgtcagc gccgccgagc
    21781 ccagtcgacg cataaagata aatccagcgg cgtagtccat gcgattgcac aatcgcccca
    21841 gccacaaagc cgtcagcgca agggctaaat cggtacgctg cacacctgaa agttgcgtag
    21901 ccaactggcg gaattccccg tcggtaaatt ccattttcag acgtaaaata tctgccgaag
    21961 cgctgcgccc cggtaaaggt gccggagaaa gtgacgcggg cggcggcagt tgacgacgct
    22021 gttctgccca gaatgccgca tcacgctgcc aggcttcgct ttcgcggtat tgctggtact
    22081 cttccactac atcagcgaaa ggcgtaaacg gcgaagcagg cgttggttca ccacgcagcc
    22141 atgtgcagta aatattggcg atctggcggg taatggccgg gaaactgaag ccatcgacca
    22201 gcaaatggtg ataacgctga taccagtacc agcggttatc cgccacctgt atcagctgat
    22261 gaaagaccag tggtttaccg ctatcgacgc gcagatcttg ttgcaaatcc gcctgcatta
    22321 atgcctgcgc agtaccgtgc ggatcaatgt tggttcgcag gtcgataatt tctggcagtt
    22381 cgaacgtcag cgcatcatcg acccactgcc agacttcgcc gttatcttcc gtaaaacgca
    22441 tccgcagcgt atctgcttgc gctagtccgt caaccaccgc gcgggccagt aatggcgcat
    22501 caacctctcc ggttaactca acgtaatgcg ctacgctcca ggcggagggt aaatccgaca
    22561 gtttttctgc catccagatg ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg
    22621 cgcctcctgc aactgggtga aattcgccgg tgtcagagta cgccagtggg cttcaagcca
    22681 ctgctggcag gactcttgtg actgcggctg acacacaatg tcccagcctg ccggtaaggc
    22741 gcattgttgc ggccacagac tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc
    22801 ctgtggatca tcgaagggat tactgaatgc catattcaac tcctgtcatg gaaaagtggt
    22861 tgccagaggt cgattagccc ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg
    22921 ccgtcaacct gacgccagaa aatggattct tttatggggt gtaattgcgc atacagcgcc
    22981 tgattggccc gcataatcat cggctcgcga atacccgctt ccagcacaat gcgcagacct
    23041 tcggcgctaa cttcaccagc tttaagcttt tcaagtaaca cgccctcttg ctgcccgccc
    23101 cgatgcggcc accagtacga tcctgactgg cttaatacac aaccaaaacg ttcaggccag
    23161 tgcagtccgg catacagcgc ggaaagtcca ccaaaactct gcccggcaac cacggtgcga
    23221 tcggcgcgat cgctaaaagg ggcaatagct ttcaccaggg gtaataactc ttgctgtact
    23281 gcgagccaga aatccgcatt acacggcagt tcgtgagcgc ggtgcgtggt gtcgatagcg
    23341 tcgatcaaca catacacggc gggaggaagt tgctgacgat gggtcagcga agtcagcact
    23401 ggccagacgg gcatactttg cgcccaaaat tcgccatcga gcaaaactgc cagcgggcgt
    23461 tcttctgctg ttgcatcgcc ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt
    23521 tcacttttcc agataatttc tttggctggt atttctggcg cttgcggaca atcccatccc
    23581 ggttgcagag gcgcttgcgg catttcgagt gcagaaacag cgtgccctcg cccgcctttc
    23641 cagctttgtg gattcagcgg atcggctatt gcctggggta atagttttcg ccagccttca
    23701 cgtaattcga ggcgatcggg ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata
    23761 aagcagtagc tgccgcgcca gttggcattg agttgtgtcg tccactgcca gacatcagta
    23821 cctgcaatgc gctgcatcga ctggggctgg ctgttctgat gatgatcggt cacaccagtg
    23881 atgtagaccc atacgcgctt tatcggcgag tattcttcag aaccttgggg atcacgccac
    23941 cagaaagtga cctcaaacat ttcgtcattc agacgctgcc attccgggcc atgtttcgac
    24001 tgccaccagc tctcacttcc tacttttaac gccgtcacac cataacccca tgtttactgt
    24061 gcaatttttc attgattaca gaaatatatt gataatatta ttgataacta tttgcatttg
    24121 caatagcgta atggcgcgcc gtgggaagcg cggacattaa ttaaccaact gcactgcgtg
    24181 tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc
    24241 cgacagttgc aattcgtggc aaaaatgcag gaataaaaca atgaacaaga agattcattc
    24301 cctggccttg ttggtcaatc tggggattta tggggtagcg caggcacaag agccgaccga
    24361 tactcctgtt tcacatgacg ataccattgt cgttaccgcc gccgagcaga acttgcaggc
    24421 acctggcgtt tcgaccatca ccgcagatga aatccgcaaa aacccggttg cccgcgatgt
    24481 gtcggagatc atccgtacca tgccaggcgt taacctgacc ggtaactcca ccagtggtca
    24541 gcgtgggaat aaccgacaga ttgatattcg cggtatgggt ccggaaaaca cgctgatttt
    24601 aattgacggc aaaccggtca gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg
    24661 cgatacccgt ggtgatactt cctgggtgcc gcctgaaatg attgaacgta ttgaagttct
    24721 gcgtggtccg gctgcagcgc gttatggcaa cggcgcggcg ggtggcgtag ttaacatcat
    24781 taccaaaaaa ggcagcggtg agtggcacgg ttcctgggac gcttatttca atgcgccaga
    24841 acataaagag gaaggtgcca ctaaacgcac caacttcagt ctgaccggcc cgctgggcga
    24901 cgaattcagc ttccgcttgt atggcaacct cgacaaaacc caggctgacg cgtgggatat
    24961 caaccagggc catcagtccg cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg
    25021 cgaaggggtg atcaacaaag atattaatgg cgtggtgcgc tgggacttcg cgcctctgca
    25081 atcactcgaa ctggaagcgg gttacagccg ccagggtaac ctgtatgcgg gcgataccca
    25141 gaacaccaac tctgacgctt acactcgctc gaaatatggc gatgaaacca accgcctgta
    25201 tcgccagaac tactcgctga cctggaacgg tggctgggat aacggagtga ccaccagcaa
    25261 ctgggtgcag tatgaacaca cccgtaactc gcgtattccg gaaggtctgg cgggcggtac
    25321 cgaagggaaa tttaacgaaa aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt
    25381 gatgctgcac agcgaagtta acctgccgat tgatttcctc gttaaccaaa cgctgacgct
    25441 gggtacggag tggaatcagc aacggatgaa ggacttgagt tccaataccc aggcgctgac
    25501 cggggcgaac accggcggcg ctattgatgg tgtgagtgcc accgaccgta gcccgtattc
    25561 aaaagcagaa attttctcgc tatttgccga aaacaacatg gagctgactg acagcaccat
    25621 cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc ggcaataact ggagcccggc
    25681 gctgaacata tcgcaaggtt taggcgatga cttcacgctg aaaatgggca ttgcccgcgc
    25741 ctataaagcg ccgagcctgt accagactaa cccgaactac attctctaca gtaaaggtca
    25801 gggctgctat gccagcgcgg gcggctgcta tctgcaaggt aacgatgacc tgaaagcaga
    25861 aaccagcatc aacaaagaga ttggtctgga gttcaaacgc gacggctggc tggcgggcgt
    25921 cacctggttc cgtaacgatt atcgcaataa gattgaagcg ggctatgtgg ctgtagggca
    25981 aaacgcagtc ggcaccgatc tctatcagtg ggataacgtg ccgaaagcag tggttgaagg
    26041 tctggaagga tcgttaaacg taccagttag cgaaacggtg atgtggacca ataacatcac
    26101 ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt ttgtcgatca tcccggagta
    26161 cacgttgaac tcaacgctga gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt
    26221 cacctggtac ggtaagcagc agccgaaaaa atacaactac aaaggtcagc cagcggttgg
    26281 cccggaaacc aaagaaatca gtccttacag cattgtgggt ctgagcgcga cctgggatgt
    26341 gacgaagaat gtcagtctga ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg
    26401 tgcgggtaat gcccagacca cgggcgatct ggcaggggcc aactatatcg cgggtgccgg
    26461 ggcgtatacc tataacgagc cgggacgtac gtggtacatg agcgtaaaca ctcacttctg
    26521 atgctaacgt cagattgttg ataaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc
    26581 cttatccggc ctacaaaaat cttgccaatt caatatattg caggaccatg taggcctgat
    26641 aagcgtagcg catcaggcag tttagcgttt gtcaccagtc tctaatatgg tcgacatgaa
    26701 aactacgcat acctccctcc cctttgccgg acatacgctg cattttgttg agttcgatcc
    26761 ggcgaatttt tgtgagcagg atttactctg gctgccgcat tacgcgcaac tacaacacgc
    26821 tggacggaag cgcaaagccg aacatttagc cgggcggatc gctgcaattt atgcgctgcg
    26881 ggaatatggc tataaatgtg tgcccgcaat cggcgagcta cgccaaccgg tgtggcctgc
    26941 ggaggtatac ggcagtatta gccactgcgg ggctacggca ttggccgtgg tttcccgtca
    27001 accgattggc gttgatatcg aagaaatttt ttctgcgcaa acagcgaccg aattgacaga
    27061 caacattatt acaccagcag aacacgagcg actcgcagac tgcggtttag ccttttctct
    27121 ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt aaggcaagtg agatccaaac
    27181 tgatgcaggt tttctggact atcagataat tagctggaat aaacagcagg tcatcattca
    27241 tcgtgagaat gagatgtttg ctgtgcactg gcagataaaa gaaaaaatag tcataactct
    27301 gtgccaacac gattaattga caacatctgg tacgattcgc ccgcagccat cactgaccgc
    27361 gggcgaaagt gtaaagcagg tgccttacca tcctgacctg acaaccggat atgcggggat
    27421 tgctccccgc acaacggcta cttcttcggt tcgtaagcga gaacagcctt aaactcaata
    27481 ttacgttcct ttacggtgaa ctcacacagc gaatccccga ccagaagcgt aaatcccagc
    27541 accgttaaac acagcactat gactgccgca agggcatatt tcgtcagcat gtttatattg
    27601 cctccatgcg aagaagggac taccatccaa attgttcagg tttgggtagg tagccccagg
    27661 gttgatagaa atatcgtcct ggggcttcat ctttccagac ctcagagtaa acctgaaacc
    27721 ggaaagcttc aggcacccgc agttatctta cccttgctta gcaaaatagc tgtttattta
    27781 tacagtaagt catgtttcgg acttctcact ccccatttga tcaactgaag ctgaacccgt
    27841 ttttttctgc atgcctcaat cgtcaaaatt cttcgccacc agtcagcact gccataactt
    27901 gtcacattgc aaaagtagcc aaattgcttc tgtcataatc agttcaccat caccaaaaaa
    27961 aagaaacatt ccagaacttc cgcatctcgc ccagccgctc ccacttctat actgaataga
    28021 aaacgccaac ataagagaaa cctatgccat tacccgattt tcatgtttct gaacctttta
    28081 cactcggtat tgaactggaa atgcaggtgg ttaatccgcc gggctatgac ttaagccagg
    28141 actcttcaat actgattgac gcggttaaaa ataagatcac ggccggagag gtaaagcacg
    28201 atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc aaccaggctg
    28261 ccgggcaatt ttcagcgatg cagaaagtcg tattgcaggc agccgcagac catcatctgg
    28321 aaatttgcgg cggtggcaca cacccgtttc agaaatggca gcgacaggag gtatgcgata
    28381 acgagcgcta tcaacgaacg ctggaaaact ttggttatct cattcaacag gtgaccgttt
    28441 ttggtcagca tgttcatgtt ggctgcgcca gtggcgatga cgccatttat ttgctgcacg
    28501 gcctgtcacg gtttgtgccg cacttcatcg ccctttccgc cgcgtcgcca tatatgcaag
    28561 gaacggatac gcgttttgcc tcctcacgac cgaatatttt ttccgccttt cctgataatg
    28621 gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctgtttcgc tgtctgagtt
    28681 acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc agtcctcatt
    28741 ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac gcggtaaata
    28801 tggcgggatt aattcaggct accgcccact ggttactgac agaacgcccg ttcaaacatc
    28861 aggagaaaga ttacctgctg tataaattca accgtttcca ggcctgtcgc tatgggctgg
    28921 aaggcgtcat taccgatccg cacactggcg atcgtcgacc gctaacggaa gacaccttgc
    28981 gattgctgga aaaaatcgcc ccttccgcac ataaaattgg tgcatcgagc gcgattgagg
    29041 ccctgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc gatttcgtcg
    29101 ccgatggtgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg gccggtgact
    29161 aagtagggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
    29221 caatgttatg aaacaccctt tagaaacctt gactaccgca gcaggcattt tgctgatggc
    29281 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
    29341 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
    29401 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
    29461 ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc ggcatacttt gcttattctc
    29521 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctgg
    29581 cccttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
    29641 aaattggcgg caaaattttt atgctgtcgt cggagctgcg cggcgtcccc tttatcaatc
    29701 tgaagtccga tccacaaaaa tccctgttaa atcagcagat atacccgagc attaagccag
    29761 ggtatcacat gaataaaaag cactggattt cggtgtatcc cggcgaggaa atctccgaag
    29821 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
    29881 gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt tttctcgctt
    29941 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attactttca catggagtct
    30001 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagta
    30061 aaaaacttac cccggaacag gccgagcaga tcaaaactct cctgcaatac agcccatcca
    30121 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
    30181 ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg taaaatactt gatgcctcgc
    30241 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
    30301 accaggaaga tgctgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
    30361 gtcgcaagtt cttcgctgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
    30421 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggct ctgggtctgg
    30481 acgcggtacc catcgaaggt tttgacgccg ccatcctcga tgcagaattt ggtctgaaag
    30541 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
    30601 acgctacgct gccgaaatct cgtctgccgc aaaacatcac cttaaccgaa gtgtaattct
    30661 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
    30721 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
    30781 ccaggttggg gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
    30841 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
    30901 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
    30961 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
    31021 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
    31081 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
    31141 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
    31201 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
    31261 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
    31321 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
    31381 ctggctggag atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
    31441 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
    31501 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
    31561 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
    31621 taaagcgcac ttgttaaaac cttatttaac cagccgccat caggaaatta atgagtggaa
    31681 tcgccagcaa ggttctacgg agtcgatatt aaacctgcgc cgaatgacca atattggaac
    31741 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
    31801 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
    31861 taccaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
    31921 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccagccg gcaatgatat
    31981 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
    32041 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
    32101 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
    32161 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg caatgcaaag atagttgccg
    32221 aacggataaa gcagcgcctt aaactgtgtt tcacgtccct gacgtcgcat cgccgcgcga
    32281 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcagcgttgc taccaccagc
    32341 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
    32401 gtgatcgctc cggaaagcat cagcgagtta atcggcacgc cgcgatggct gacgcgagtc
    32461 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
    32521 accccgctgt tatacactga cagcgatgct accagaatga cgaagttcag cgcagaagct
    32581 acctcgttgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
    32641 tccacccacg gatagagcgc cagtaaaacc accagtgaac agatgtaaaa cagcaggatg
    32701 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
    32761 gcagtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
    32821 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
    32881 gctttctcgc cgccgtgacc agaaaacagc agccacaggc caaagccgat cataccgatg
    32941 attgccagca ctttaatcaa cgcaaaccag aactcggttt cgccatataa gcgcacgttc
    33001 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
    33061 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
    33121 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
    33181 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccgagctgg
    33241 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
    33301 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
    33361 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgatttgac
    33421 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
    33481 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
    33541 acggggtatc ctgcttttat ttccgtaccc gatatcggtg cagccacatc agcttatacg
    33601 ccgccgggat aataaacagc gacagcaaag gcgcggtgat catgccgcca atcatcggtg
    33661 cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac
    33721 cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcagtacc gcgccgtgat
    33781 acaacgcctc atccagcttc tgctcactga atgtttgcgg attattcaac gacggctcgg
    33841 cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgcccccgg
    33901 caagggcaat aaaaccggtg cccgttgcca cagaaagatg aaagcccatc caccacagca
    33961 accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca
    34021 cccgacggaa tgccagatac aacagcacga agatgatcat cagtgtcatc ggcaccatga
    34081 gtttcagctt atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg
    34141 tgcccggttt taactgcact ttttcaacta tcgctttttg caaatcgtga accaccgaca
    34201 ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt
    34261 tcagcatcga cggtccggta gagactttaa cgtcggccac gtctgccagg gtgatttgct
    34321 gcttcatcgg cgtcaggatc ggtagctggc gcagtgcctg cgggctatcg cgccagcttt
    34381 gcggataacg cagattaatg ggataacggg caatcccttc caccgtttcg ccaaccatcg
    34441 ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgcg
    34501 cggctttttc acggttaatc tcaacgttga tatagcgccc accttccaga cgctcggcaa
    34561 gcgcagaagc cacgcctggc accgttcgcg ctacttcttc aatttgctca gccatcgcgt
    34621 cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta atgccggttg
    34681 agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagcc
    34741 gcacggtgtt atccagttcc tcaatgattt tgtccatcgt catgccaggc cgccactgat
    34801 cctgcggctt aagctggatg gtcgtttcaa ccatctcgag cggagcagaa tcggtggcgg
    34861 tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct
    34921 tgtcgttttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg
    34981 gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga
    35041 gcggccagag aaccgtcagc accgaaagcg ctgccaccaa cagcgtggtt ttcggccagt
    35101 gcagtacttt cagcaataga ggatgataaa cacgaatcaa aaagcgattg agcgggttac
    35161 tgctttccgg cggaattttg ccacggatcc agtaacccat caggatcggg atcactacga
    35221 tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccagc gggccaaaca
    35281 gacgcccttc ttgcccttcc agggtgaaga ttgggataaa cgacagcgtg ataatcagca
    35341 gactgataaa cagcgccagc cccacttcaa cagatgcatc ggtaatcacc tgccagcgcg
    35401 ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgcg
    35461 cattctcgat catgacgata gcagcatcga ccatcgcccc gacggcaatc gcaatgccac
    35521 ccaacgacat aatattggca ttcagtccct ggaagggcat gacaataaaa gcaatacaca
    35581 accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg
    35641 cacagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg
    35701 cgcggtcaat gagctggctg cgatcgtatg ttgtaactat ctccacgcct tccggcagac
    35761 tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg gcgtttttgc
    35821 cggagcgcag gatcaccacc ccgcccgcca cttcgccttc gccgttcagt tcggcaatgc
    35881 cccggcgcat ctccgggcca acctggacct tcgcaacatc gcgcagataa acgggcacgc
    35941 cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc agatagccgc
    36001 tggcgcgcac catatattcc gcttccgcca gttcgatcga cgaaccgccc gcttcctggt
    36061 ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gtcatactgc gccaggcgct
    36121 ggggatcgat aaccacctga tactctttca ccacaccgcc caccgacgcc acttccgcaa
    36181 cgtcagggat ggttttcagc tcatatttga gaaaccagtc ctgtaatgag cgtaaatcgg
    36241 ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg
    36301 tggcatctgg ccccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga
    36361 ggtactccag cacccgcgaa cgcgcccagt acggatcggt accatcttcg aaaatgacat
    36421 acacataaga gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca
    36481 acatggtggt ggttagcgga taagtcacct gattttcaac gatttgcggt gcctgcccgg
    36541 gatagctggt tttaataatc acctgcacat cggagagatc cggcagcgca tccactggcg
    36601 tattaatgat ggtccaggtg ccccagatgc taagaaacaa cgcgcccatc agcaccaaaa
    36661 aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc
    36721 atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc
    36781 aatcaggaaa aggccgctgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc
    36841 ggtgacacct tgcgatgcct ggaaaacggc aacgcgtttc ggtacaaagc gcccgtcggc
    36901 atcaatggta atcacccgct gttcgctacc ggtatcaatc agcccttgtg acggaatgag
    36961 cagcatcggt tcgctggcgg tattgagttg caaccaggcg ttcatgcccg gttttagcgc
    37021 ctcgtcggca ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc
    37081 gggtagcagc gtccatttgc ggatggtgag tgttttatcc ggtcgtgccg gaacggtgag
    37141 cgtaaactgc gaggcatctt tcaccagcca ggcgatagac tccgggatcg cagcagtgac
    37201 ccacaccggg tccataccct gaattttcgc taccacgtta tctttggcga tattcattcc
    37261 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
    37321 ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc ccgccagtcg
    37381 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcagcagtaa
    37441 atactcactc tgcgcttcca cccagtcagg aatggtcagg tcgagaagcg gtgtgccctt
    37501 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
    37561 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
    37621 agtcagcggt ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
    37681 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
    37741 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttggggtaca tcgggtcgta
    37801 ccagaataag attttacgt
//
LOCUS       DADLTD010000012        37234 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLTD010000012 DADLTD010000000
VERSION     DADLTD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.012"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..808)
                     /locus_tag="JN701_000479"
     CDS             complement(<1..808)
                     /locus_tag="JN701_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HAY7363022.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVNHA"
     gene            complement(822..1253)
                     /locus_tag="JN701_000480"
     CDS             complement(822..1253)
                     /locus_tag="JN701_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363023.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1253..1456)
                     /locus_tag="JN701_000481"
     CDS             complement(1253..1456)
                     /locus_tag="JN701_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAY7363024.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1569..2417)
                     /locus_tag="JN701_000482"
     CDS             complement(1569..2417)
                     /locus_tag="JN701_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363025.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILDIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2513..3772)
                     /locus_tag="JN701_000483"
     CDS             complement(2513..3772)
                     /locus_tag="JN701_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7363026.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3963..4826)
                     /locus_tag="JN701_000484"
     CDS             complement(3963..4826)
                     /locus_tag="JN701_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY7363027.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4953..5669
                     /gene="rph"
                     /locus_tag="JN701_000485"
     CDS             4953..5669
                     /gene="rph"
                     /locus_tag="JN701_000485"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY7363028.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5735..6376
                     /gene="pyrE"
                     /locus_tag="JN701_000486"
     CDS             5735..6376
                     /gene="pyrE"
                     /locus_tag="JN701_000486"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY7363029.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6413..7009)
                     /gene="slmA"
                     /locus_tag="JN701_000487"
     CDS             complement(6413..7009)
                     /gene="slmA"
                     /locus_tag="JN701_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY7363030.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7116..7574)
                     /gene="dut"
                     /locus_tag="JN701_000488"
     CDS             complement(7116..7574)
                     /gene="dut"
                     /locus_tag="JN701_000488"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY7363031.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7552..8772)
                     /gene="coaBC"
                     /locus_tag="JN701_000489"
     CDS             complement(7552..8772)
                     /gene="coaBC"
                     /locus_tag="JN701_000489"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7363032.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8944..9612
                     /locus_tag="JN701_000490"
     CDS             8944..9612
                     /locus_tag="JN701_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY7363033.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9829..10065
                     /gene="rpmB"
                     /locus_tag="JN701_000491"
     CDS             9829..10065
                     /gene="rpmB"
                     /locus_tag="JN701_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY7363034.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            10086..10253
                     /gene="rpmG"
                     /locus_tag="JN701_000492"
     CDS             10086..10253
                     /gene="rpmG"
                     /locus_tag="JN701_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY7363035.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10351..11160
                     /gene="mutM"
                     /locus_tag="JN701_000493"
     CDS             10351..11160
                     /gene="mutM"
                     /locus_tag="JN701_000493"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY7363036.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11199..11678)
                     /gene="coaD"
                     /locus_tag="JN701_000494"
     CDS             complement(11199..11678)
                     /gene="coaD"
                     /locus_tag="JN701_000494"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY7363037.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11686..12963)
                     /gene="waaA"
                     /locus_tag="JN701_000495"
     CDS             complement(11686..12963)
                     /gene="waaA"
                     /locus_tag="JN701_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY7363038.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13376..14434
                     /gene="rfaQ"
                     /locus_tag="JN701_000496"
     CDS             13376..14434
                     /gene="rfaQ"
                     /locus_tag="JN701_000496"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY7363039.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14431..15555
                     /locus_tag="JN701_000497"
     CDS             14431..15555
                     /locus_tag="JN701_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY7363040.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15548..16345
                     /gene="rfaP"
                     /locus_tag="JN701_000498"
     CDS             15548..16345
                     /gene="rfaP"
                     /locus_tag="JN701_000498"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY7363041.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16361..17377
                     /gene="waaO"
                     /locus_tag="JN701_000499"
                     /gene_synonym="rfaI"
     CDS             16361..17377
                     /gene="waaO"
                     /locus_tag="JN701_000499"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY7363042.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            17394..18389
                     /locus_tag="JN701_000500"
     CDS             17394..18389
                     /locus_tag="JN701_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HAY7363043.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            18399..19091
                     /gene="rfaY"
                     /locus_tag="JN701_000501"
     CDS             18399..19091
                     /gene="rfaY"
                     /locus_tag="JN701_000501"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY7363044.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            19117..20142
                     /locus_tag="JN701_000502"
     CDS             19117..20142
                     /locus_tag="JN701_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HAY7363045.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            20227..21210
                     /locus_tag="JN701_000503"
     CDS             20227..21210
                     /locus_tag="JN701_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7363046.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            21256..22509
                     /locus_tag="JN701_000504"
     CDS             21256..22509
                     /locus_tag="JN701_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAY7363047.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(22579..23559)
                     /gene="rfaC"
                     /locus_tag="JN701_000505"
     CDS             complement(22579..23559)
                     /gene="rfaC"
                     /locus_tag="JN701_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY7363048.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAENAAMI"
     gene            complement(23563..24609)
                     /gene="rfaF"
                     /locus_tag="JN701_000506"
     CDS             complement(23563..24609)
                     /gene="rfaF"
                     /locus_tag="JN701_000506"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY7363049.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVKRYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(24619..25551)
                     /gene="rfaD"
                     /locus_tag="JN701_000507"
     CDS             complement(24619..25551)
                     /gene="rfaD"
                     /locus_tag="JN701_000507"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY7363050.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25765..26961
                     /gene="kbl"
                     /locus_tag="JN701_000508"
     CDS             25765..26961
                     /gene="kbl"
                     /locus_tag="JN701_000508"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY7363051.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26971..27996
                     /gene="tdh"
                     /locus_tag="JN701_000509"
     CDS             26971..27996
                     /gene="tdh"
                     /locus_tag="JN701_000509"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY7363052.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            28235..29269
                     /gene="waaH"
                     /locus_tag="JN701_000510"
                     /pseudo
     CDS             28235..29269
                     /gene="waaH"
                     /locus_tag="JN701_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(29256..30215)
                     /locus_tag="JN701_000511"
     CDS             complement(29256..30215)
                     /locus_tag="JN701_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY7363053.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVRLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(30219..31502)
                     /gene="envC"
                     /locus_tag="JN701_000512"
     CDS             complement(30219..31502)
                     /gene="envC"
                     /locus_tag="JN701_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY7363054.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(31512..33056)
                     /gene="gpmM"
                     /locus_tag="JN701_000513"
                     /gene_synonym="pgmI"
     CDS             complement(31512..33056)
                     /gene="gpmM"
                     /locus_tag="JN701_000513"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY7363055.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            33301..33732
                     /locus_tag="JN701_000514"
     CDS             33301..33732
                     /locus_tag="JN701_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY7363056.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            33874..34125
                     /gene="grxC"
                     /locus_tag="JN701_000515"
     CDS             33874..34125
                     /gene="grxC"
                     /locus_tag="JN701_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY7363057.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            34188..34655
                     /gene="secB"
                     /locus_tag="JN701_000516"
     CDS             34188..34655
                     /gene="secB"
                     /locus_tag="JN701_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY7363058.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            34655..35674
                     /gene="gpsA"
                     /locus_tag="JN701_000517"
     CDS             34655..35674
                     /gene="gpsA"
                     /locus_tag="JN701_000517"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY7363059.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            35754..36575
                     /gene="cysE"
                     /locus_tag="JN701_000518"
     CDS             35754..36575
                     /gene="cysE"
                     /locus_tag="JN701_000518"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY7363060.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(36628..37101)
                     /gene="trmL"
                     /locus_tag="JN701_000519"
     CDS             complement(36628..37101)
                     /gene="trmL"
                     /locus_tag="JN701_000519"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY7363061.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         9224 a         8863 c         9274 g         9873 t
ORIGIN      
        1 acgcatggtt tacctccaga cgtttagcga gccagcgttg cgacaggcgg gtgagttctc
       61 ctttgcgctg ctcatattcc atgcccatat caattaacgt gatattggtg ctttcgagat
      121 aggcgaggtg ttcaagctgt gctgcgttca tgctgtcccg tggttcgcca gtaatgccat
      181 ttgcctgcgc ccactgcttc gccgtcatgc cacccagcac gatacgggcg atcatgttgc
      241 tctcgttgct gtagtggcgc tgctgcgtcg ttttgcccat ctcggcacgg gccatattca
      301 gcgcatcgca cattggctta aagttgtttg cggcactgag acgggctttt agctggcgac
      361 gatagcgggc ggcgatttca ggcacgctac gctgtaattc ttcctcgcac tggatgaaat
      421 aacggcggat agcgcggccc tgctcgttgc gttcaaccat tgccacttct ttagccatat
      481 ccagcgatag caggtaatca tgctctattt gctggcgagt ttttgcgctc gcccgttttg
      541 gcgtgctcaa attttcaaca cggataaaat cggttccggc aataaaaccg tactggtcaa
      601 ttcggccttt aatccagtta gtaaagtcgc ggcccacccc cagcgcttta tgcaacgcct
      661 tagcgctcac aatattggct tcacgcccgc caatgcgtcc ggcaataacg ggaacaatag
      721 cggcaaagtc gttgccgtta attactcccg tgttaacgtt gggttgcggg gcggcccccg
      781 aactgaatcg gcttttttcg atagtcatat tttcggctcc gttatgcggc ggtgagattg
      841 tccgggtata gattgagaat gtcggcaatt tcaggctgag aaagtccctg atagccgcca
      901 gcgtcggcgt tatggttcac aagccgaata acttttagaa cgtcaccgcg cccggtaaag
      961 cgatagcgta aatgtgaccc tatcccgtcg gggttctttt cgtctatacg ctcaagctga
     1021 atatcaagcc tgcgctctaa ctcgctggcg tagttccttc cagacgaaag gcggcaataa
     1081 cgcagaatgt cattttctgt ccatccctct ataccagtac gcagcatata gactctggca
     1141 cgatgctttt tcggtgtgcg ctttggtgct tgaagggatt ttgtaagggg cgtaacatca
     1201 gatacgcgaa tatctgaatt agccgcctgt aatgggcggt ttttcttttc catcaggcca
     1261 ccttcccgcg gcgttctgcc agccagttgt gaatctcaac cgcatcaaac gcagttattt
     1321 tttctcccag ctttaccgga cgtggtagtg aaccgtttct tactttacgg tcgatggtgg
     1381 atacactaac gcccagcaat tcagccaggc ggaagcgtct gatatagcct tttgtaggga
     1441 tagtgtcgat gttcatgatt cccgtaacct cttagcgata gttgttgctt cgttacgggt
     1501 atttaatggc gtgcacgctc aaaaaggaat gatcgtattt tttactatcg tgatagtaaa
     1561 atttactatc accttttttt ccctgccgtg gtgcgttctt ccggtgttct gtctgcgtcg
     1621 gctatgagtt tcttcaggta atctatggat ggctcatcat agccgccccc caaaagcgcc
     1681 cagtggttta ttgtggcgtt cgcccatgct tcgttggttt ccttcccgtt cttcccttta
     1741 cactcttctg ggtagtgccg cttcacataa atcaacgcca agaggcaaat ttcgcgcttt
     1801 tgcctgtggc gctctctgtt tccgtgctca acgtttttaa catcctcata aagagcttgt
     1861 aagtttatgc gagtttcggg gctgtatatg ggctggtttt tgtccatgca ttcgccgata
     1921 tattcaaccc acggccttgt tatgtacaaa tcctctacgg atatttcgtg gaaggcatgt
     1981 tttgtataat cgagaaaaaa ataataattc gcacttacgt ctagttctct ttttctgtgt
     2041 gcggggctga atatgcgtgc gggagtgtca atagaatcaa gccctgcgat gtcgagtata
     2101 taggaggcgt taacatgcca tagcccaagc acatggcaat ctatatgatt tacaacgcct
     2161 gccttaaagc ttttttcgtc ctctacttca gtgattatgt cggcaagtaa taagcagtct
     2221 tctttgacgt ctgggaataa agagcaattg cggtaaagac tcgcgatgct atgtaatgtg
     2281 ccgtcgtagc cgcaaaggta cgcgctcaaa tatatagatt ctgccgccgc cataatcaaa
     2341 agatcatcaa tatctatttc taataattcg gctgctctgt taagcctgaa aaaaggggcg
     2401 ggtggtaatt ttttcattgt gtcacctttt acgctgcgcc ttattttgtg attgagccag
     2461 gcggtaaggc ttcccgcttt tcgccccgtc gagctaggct caatctattc agttagttcg
     2521 cccttctgag aggcgttacg ttgtagtctt ttccgctttc cagtgccacc agcagatcag
     2581 cccactgtgc cagtgctgct ttacgctcat caaaatactg atggcggtta taaatcccct
     2641 ctacgccctt aatgcgatga ttgaggcatc gttccgccac aacaggatca acacccaacg
     2701 ctgctaaatg agtacgtgcg gtgcgtcgaa agtcgtgaat cgtgaaatta ggcacatccg
     2761 gcatttcggc gcgaacctta gccagtgcta cgggtaaagt gctttcctga atatgtggga
     2821 tcattctgtt ttgcatcttc ctggccggaa gcacccatgc gctattacat gaaaaggtgt
     2881 gtagctctct caaccattca acggctgggg aaggtagagg tatatcaata gggtctccgt
     2941 ttttgctgcg ttcttccggc aaatgccata ccgcaccatc taaatcaaac tcttcccatc
     3001 gtgcggcgca taattccatt ttgcggacgc ataacgctaa cagtaatttg aacgtgattt
     3061 cgttctggcg actgaatccc ttagccgtgc gcatggcttt gaatagctgg attaactcat
     3121 cgcgggttaa ccagcggtca cgggcagctt ctttccctcc ggcgtctgcc acctcaaagg
     3181 ctgaacaggg gttaatctct agcgcgtgcc gtttgattcc gtagtcgaat atgcggcgcg
     3241 tccatctcag cacgtccgtt gctatggtcg gcgctccacg gtcaacaata cctttcagca
     3301 tgtcatcgat atggcgcggt ttcacgtcct ctaccttcat gctgccaatg caggggttta
     3361 tatctttgtc gatacggcgg cggagtatat cggggtgctt ccagcgcggg aggatctgac
     3421 gctcaaagta ttcagcggca agctctgaaa cgcgcatagc gttcttctct gcttccatct
     3481 tcgccagtgc ttcggttttg cgcttctgct tctctcctgc aacgtcatag cccagcgcaa
     3541 cgcgagccga tagctctttg gctgtctctc tggcctttga tagtgatagc tcgctatacg
     3601 aaccaattag catggcgcgg gacttccctg cgagtttata gcggaatctc catgtggggg
     3661 ttttgtcggc ttcacggtaa cgtaggtata aaccgttacc gtctgcccgc ccctcgaatc
     3721 gctctccgct cttaatccat gcgcggatct gcatgtctgt tagttttggc attttgcgaa
     3781 tatccaaact ttgaataggg ggtgtaccca aagcaatgta cccaaacctc attgggtaca
     3841 cctttgtgta caccataatc atgcgctttg ttgcgtcttt atgatagccc atgataacaa
     3901 aaaagcccgc tgggttgcgg gcttggtgtg atttatgcgt cgtcctaaat gttgctgata
     3961 gcttactcga tattctgaat ctgctcgcgc atctgctcaa tcaacacttt cagctcaatc
     4021 gcggagtttg tcacttcggc attgatagac ttcgacgcaa gagtgttcga ctcgcggttg
     4081 aattcctgca tcataaagtc cagacgacga ccaacggctt cttttttctt cagaatgttg
     4141 taggtctctt tgacatgcgc ttccaggcgg tccagttctt cggcaacgtc aattcgttgt
     4201 gccagcagaa ccagctcctg ctccagacgg ttgttttcca actgaacctg agcatcttcc
     4261 agcttcgcga ccagacgctc acgctgccat tgcaggattt ccggcatatg ggcgcggact
     4321 ttgaccactt cggcggtgac gccttccaga cgctgctcga tcaatgcttt cagtgcctga
     4381 ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac catccagcgc cgcgagaatt
     4441 tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca tcacgcccgg ccagcgtaga
     4501 atatcaaccg ggttgatttc cccttcgtca ctctgcattt ttacccagtt cgcggcagtt
     4561 accagctgtt tagccagttt ttcgttgagg atcagctcac cttgcgcgct aatatctggc
     4621 tcatagcgca gggtacattc cactttaccg cgcgtcaggc gagaacgaat acgctcgcga
     4681 acgacaggtt caaggctacg gaactgctcc ggcagacgaa agtaagtttc cagataacgc
     4741 tggtttaccg agcgcatttc ccaggttgcg ctcccccatt cacccttgat ttcacgccgg
     4801 gcgtaggcgg tcatactgcg gatcatagac gttcctgttt ataaaaggag aggtggaagg
     4861 attatagcca tcgatgcctt gtcaggatag gaataaccgc cggaagtccg tataatgcgc
     4921 agccacattt gtttcaagcc ggagatttca atatgcgtcc agcaggccgt agcaataatc
     4981 aggtgcgtcc cgttaccctg actcgtaact atacaaaaca tgcagaaggc tcggtgctgg
     5041 tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat tgaagaaggc gtgccgcgct
     5101 tcctgaaagg tcagggccag ggctggatca ccgcagagta cggcatgctg ccacgttcta
     5161 cccacacccg taacgctcgt gaagcggcga aaggtaagca gggtggacgc acaatggaaa
     5221 tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga tttgaaagcg ctgggtgagt
     5281 tcaccattac gctggactgc gacgtgcttc aggctgatgg tggcacgcgt accgcgtcga
     5341 ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa gctggtggaa aacggcaagc
     5401 tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc tgtcggaatt gtgaacggcg
     5461 aagcggtttg cgatctggaa tacgttgaag actctgccgc agagaccgac atgaacgtag
     5521 tgatgaccga agacgggcgc atcattgaag tgcaggggac ggcagaaggc gagccgttca
     5581 cccatgaaga gctactcacc ttgttggctc tggcccgagg gggaatcgaa tccattgtag
     5641 cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg actgatgagt cgcctttttt
     5701 ttgtctgtag aaaagtaaga tgaggagcga aggcatgaaa ccatatcagc gccagtttat
     5761 tgaatttgcg cttggcaagc aggtgttaaa gtttggcgag tttacgttga agtccgggcg
     5821 caaaagcccc tatttcttca acgccgggct gtttaatacc gggcgcgatc tggcactgtt
     5881 aggccgtttt tacgctgaag cgttggtgga ttccggcctt gagttcgatc tgctgtttgg
     5941 ccctgcttac aaagggatcc cgattgccac cactaccgcc gtggcgctgg cggagcatca
     6001 tgaccttgac ctgccgtact gctttaaccg caaagaggca aaagaccacg gtgaaggcgg
     6061 caatctggtt ggtagcgcgt tacaaggacg cgtaatgctg gtagatgatg tgatcaccgc
     6121 cggaacggcg attcgcgaat cgatggagat tattcaggct aatggcgcga cgcttgctgg
     6181 cgtgttgatt tcgcttgatc gtcaggaacg ggggcgcggc gagatttcgg cgattcagga
     6241 agttgagcgt gattacaact gcaaagtgat ctctatcatc accctgaaag acctgattgc
     6301 ttacctggaa gagaagccgg aaatggcgga acatctggcg gcggttaagg cctatcgcga
     6361 agagtttggc gtttaaagaa actcgccgga tgaaaagtca tccggcgtca tattactgca
     6421 actgtgccgc aattagcggc cagcgggcgt caaaatcatc cgtcgggcgg tatttaaatt
     6481 cgctgcggac aaaacgtgac agcatacctt cacagaaggc caggatctgg cttgccagca
     6541 gggtttcatc ggtggtgtaa ccttcaccct cacgcattct cttttcacgc aatacctgac
     6601 gcagctgcgc ttcaatacgc tcgaacagct ggttgatgcg cccttgcagg cgatcctgtt
     6661 caaacattag cgcatgacca gtgaggatgc gggtcaggcc aggattacgc tcaccaaaac
     6721 cgagaagcag caacacaatc agacgcaggc gcgctgtggt gtctttctca tctttcagaa
     6781 tcaggttgat gcgagtaatc aggctatctt cgataaactc aatcaggcta tcgaacatgc
     6841 gggtcttact ggggaagtgg cgatacagtg ccgcttcgga aacgccgaca gaggcggcca
     6901 gttttgccgt cgtgatacgt tggcttccat cgctggattc cagcatcagc gccagagact
     6961 gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt ttctgccatg ttacaaaata
     7021 cccctgaaaa taagcacttg ccaggcggca cccacgctat gaccgcaaac gaaatgtttg
     7081 cggctatgtt atgacgttat tcggatgcgt atgtgttact gacgaccaga gtgaccaaag
     7141 ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca gattaaattc agcctgtact
     7201 accggaacaa aaatcatctg ggcgatgcgt tcgccaggtt gaatggtgaa gctgtcctga
     7261 ccacggttcc acacggaaat catcaactgg ccctgatagt cggaatcgat caatcctacc
     7321 aggttaccaa gcacgatacc gtgcttatgt cccaatccgg agcgcggcag catcattgcc
     7381 gccagtgaag gatcggcaat atgaatcgcc agcccggtcg gaaccagcgt agtgtcaccc
     7441 ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt caagtccggc agagccagag
     7501 gtggcataag tcgggagcgg aaattccttc ccaacgcgcg ggtccagaat cttaacgtcg
     7561 atttttttca tcataacggg tcacgatctc gtcgagtaat agttggccaa ggagctcttt
     7621 gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa aggtgtaatg cgttgttttc
     7681 gctgttaaat ccttgagttg gctgggaaac atcgttcgcg cagatcagat caaggttttt
     7741 acggatacgt ttttgccggg cgtattcttc cacattattt gtttcggcgg caaatccaac
     7801 gacgtagggt cgatggtctt ttagtgcggc aacgcctgcg acgatatcgg ggtttttaac
     7861 catttttatt gttaattcat caccctgcgt ggcctgcttt ttgatttttt ctggggccac
     7921 ggtagctgcg cgataatccg ccacggcggc gcagccgata aaaatatttt gctgctgtac
     7981 agaagcattc acggcggctt ccatttccag cgcggtcatc acatcaacac gtttaacaaa
     8041 cggtggcgtc ggtagtgaaa ccggacctga taccagcgtg acgttcgcgc cacgacgggc
     8101 agcggcggcg gcgatagcaa aacccatctt gccggagctg tgattagaga tataacgcac
     8161 gggatcgagc ggttcacgcg tcggaccggc ggtaatcata atgttcagat gtttcaggtc
     8221 gttgacgggc gaaaaatgcg ccaccgccat atccacaatg gttaacggat cgagcattcg
     8281 cccaggaccg atatcaccac aagcctgact gccactgtct ggcccccaga tgagcaaacc
     8341 acgggaagca agcccctcta aattatgctg cgtggcagcg gcacggtaca tctgctggtt
     8401 catggcgggg agcacggcta caggcgcagg tgtagccaga caaatcgtcg ataccaggtc
     8461 attcgccatt ccggcagcaa cacgggcaat caaatctgcc gtggcagggg cgagaatcac
     8521 taaatcagcc catttaccca gctcaatatg gcccatagcg gcttcggctg ccgggtccag
     8581 caaactgtcg gaaacgggat aaccagaaac cgcctgcaag ctaagtgggg tgataaaggc
     8641 ttttgccgct tcggtcatgg ctacgcggac gtcggccccg cgatcgcgca aacgacgcac
     8701 cagttcaggg gttttatagg cagcaatacc gccgctaacg ccgagaacga tttttttacc
     8761 ggccaggctc atgatgattt ttcctgttgg gtgacaccag aagttggcga ttttatcaca
     8821 atacttttgt tgtcgtgcct tcacctgaga atcactttgc gaggcgcttt ccaggattga
     8881 aaactggccg tcgatttaac ggaacggcta tgacaggatg cgagcaccac aaaggaggtg
     8941 aaggtgaaaa acaattcaca gctgttgatg ccgcgcgaaa aaatgctgaa gtttggtatt
     9001 agcgccttaa cggatgtcga gctgctggcg ctatttctgc gtaccggaac gcgcggtaaa
     9061 gatgtattaa ccctggcaaa agagatgctg gagaatttcg gctctcttta tggcttgtta
     9121 acctctgaat atgaacaatt tagtggcgtt catggaattg gcgtggcgaa atttgcccag
     9181 ttaaagggga ttgctgaact ggcgcggcgt tactacaacg tgcggatgcg tgaagaaagc
     9241 cctttactca gcccggagat gacgcgggaa tttttacaaa gccagcttac gggtgaggag
     9301 cgggagatct ttatggtgat ctttctcgac tcacaacacc gggttataac gcatagccgt
     9361 cttttttccg gcacgctaaa ccatgttgaa gtccatcctc gggaaattat ccgcgaagcg
     9421 ataaaaataa acgcctcggc gctgatcctt gcacataatc acccttcggg ttgtgctgaa
     9481 cccagtaaag cggataaact cattactgaa cggataataa agagttgtca gttcatggat
     9541 ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt atgtttcttt tgccgaacgc
     9601 ggctggattt aacccgctat gcgcgatcct tcgggatctt tgtctgttcg ggacttgagc
     9661 acatcgctga gtcagcgtat actacgccac ctttgagaat ctcgggtttg gcatttgggc
     9721 ctggcaatcg agagttcaca tagaactgcg atgactgggc tgtaaagcct gacgaggcgc
     9781 caatacccca tacgaagctc gagctaattt gatttttgga gaatagacat gtcccgagtc
     9841 tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc gttcccacgc actgaacgcg
     9901 actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt tctgggttga gagcgagaag
     9961 cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg taatcgataa aaaaggcatc
    10021 gatacagttc tggctgaact gcgtgcccgt agcgaaaagt actaagtact tagaggaaat
    10081 aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt ttcttctgct ggtactggtc
    10141 acttctatac cactacgaag aacaaacgta ctaagccgga aaaactggaa ctgaaaaaat
    10201 tcgatccagt tgttcgccag cacgtgatct acaaagaagc gaaaatcaaa taattctcgc
    10261 tttgatgtaa caaaaaacct cgctccggcg gggttttttg ttatctgctt gcccccatat
    10321 tgactgcatc tgttcattcc tggagatgct atgcctgaat tacccgaagt tgaaaccagc
    10381 cgccgcggca tagaaccgca tctcgttggt gcaaccattc ttcatgcagt ggtgcgcaac
    10441 ggacgcttgc gctggccggt ttcagaagag atctaccgtt taagcgacca accagtgctt
    10501 agcgtgcagc ggcgggctaa atatctgctg ctggagctgc ctgagggctg gattatcatt
    10561 catttaggga tgtctggcag cctgcgcatc cttccagaag aacttccccc tgaaaagcat
    10621 gaccatgtgg atttggtgat gagcaacggc aaagtgctgc gctacaccga tccgcgccgc
    10681 tttggtgcct ggctgtggac caaagagctg gaagggcata atgtgctggc ccatcttgga
    10741 ccggagccgc ttagcgacga tttcaatggt gagtatctgc atcagaagtg cgcgaagaaa
    10801 aaaacggcga ttaaaccgtg gctgatggat aacaagctgg tggtaggggt agggaatatc
    10861 tatgccagcg aatcactgtt tgcggcgggg atccatccgg atcggctggc gtcatcactg
    10921 tcgctggcag agtgtgaatt gttagctcgg gtgattaaag cggtgttgct gcgttcgatt
    10981 gagcagggtg gtacaacgct gaaagatttt ctgcaaagtg atggtaaacc gggctatttc
    11041 gctcaggaat tgcaggttta cgggcgaaaa ggtgagccgt gtcgggtgtg cggtacgccg
    11101 attgtggcga ctaaacatgc gcagcgggca acgttttatt gtcggcagtg ccagaagtaa
    11161 ttcatgcgcg ccggatcgca taccatccgg cataaacgct acgctaactt cgccatcagc
    11221 gcctgatgga cattctccgg caggaaatgg gtgacatcgc cctgatggcg cgccacctct
    11281 ttcaccaacg atgaagagat aaacgaccac tctttcgacg gcatcagaaa cacactttcc
    11341 agttccggca ttaagtggcg attcatatgc gccagctgca tttcatattc aaaatccgcc
    11401 accgcacgca ggccacgaat cagcaccgta gcgtgttgat tacgggcgaa gttcgccatc
    11461 aaatcactaa acccaactac ttccacgttc cccagatgcg cggttgcctg ctgtgccagc
    11521 gccacacgct cttccagggt aaacatcggt tttttactgg ggctggcggc aatcgccaga
    11581 ataacgtgat cgaacatttg cgtggcgcgc gtcacgatat cgatatgacc attggtaatg
    11641 ggatcgaaag tacccggata aatcgcccgt ttttgcataa caacctcaat gcgttttcgg
    11701 tggcaggtaa ggttccagca gttgaagcag gcgctgtagc gcgccctggt tttgatacag
    11761 tacttcaacg gcatgacggc catagaaact acggtaatcg gcgtcggtga gtaaagagga
    11821 aacctctttt gcaagcgtag tggcatcggt aacggtaatc agcccgcttg cctgctccag
    11881 ccgcgcgcaa atgtctttaa aattaaaagt atgcggcccc atcaataccg gaatagcgtg
    11941 tgcggcagct tccagcggat tatgcccacc acgttcaacc agtgaaccgc caacaaaggc
    12001 gagatcggca atgccgtaca gtaacatcaa ctcgcccatc gtatcgccaa ccacaacctg
    12061 cgtgctggtg gaggggactt cccctgaaga gcgtgtgata tagcttagtc cagcctggcg
    12121 gacaaggtta atcgcatccg ggaagcgttc tggatgacgg ggtaccagga tgagcaataa
    12181 attcgggaat tgctgtaaca atacctgatg tgcggcgatc accacactct cttcaccttc
    12241 gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc cactggcggc gcagcgtcac
    12301 ggctttagca gccaactgcg gcgttacaga aatatcgaat ttcaggctac cggtaacggt
    12361 tacctggtta ttttttgcgc ccagcgccac aaaacgtgca ccatcttctt cattttgggc
    12421 ggcaatcagc gtaatacgac gcagcaagcg acggacgaat ttacccagtt tggcataacc
    12481 tgcggccgag cgggcagaga gtcgcgcgtt cgcgatcacc agcggaattt tacgtttatg
    12541 tagcgccgca atcaggttag gccatagttc ggtttccata atcaacacca gtttagggtc
    12601 gactttattc aggaaacgat taagtgcatc gggcaggtca tacggcagat aaacgtgctg
    12661 aacatccttc ccgaaagccg attgtacgcg ctccgaaccg gttggcgtca tggttgtaac
    12721 ggtaatcggt aaatcaggat aacgatgacg cagcgcgcgc accaacggga tcgccgccag
    12781 agtttcaccg acggagacgg agtgcagcat aatgccgcct ggttttagcg gatggcggta
    12841 aaaaccgtaa cgttcacccc agcgttttcg ataggccgga gccttacgtc cgcgcaccca
    12901 gagccgtatc cagatcagcg gctgaataag gtagagaagg gcggtgtaaa gcaattcgag
    12961 catagtaaat agctgactta tggatgtgct ggggattcta tgtatttagc tgtggcttta
    13021 ccattacttt tcccgttttt gacttaaata gcaatttacc cgccaattct tgctgaacat
    13081 ctatactttt tttgtatttt tatgcgattc attgatttct gctgtacgtg caggaaggaa
    13141 tcgcagagca acagtgaaag aatatggttg ctaatgattt tatttgattg atttctttat
    13201 tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat tgccgcgaaa gaagtgatac
    13261 actagcgcgt caatatcagt gcggtatcag tattcaggta gctgttgagc ctggggcggt
    13321 agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa caaaaagagt tacttgtgga
    13381 taagctattt cgaagaattt tgctcattaa gatgcgtttt catggggata tgttattaac
    13441 tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg atgtgctgct
    13501 ttatcaggac accatcccga tcctgtctga aaatccagag attaacgcgc tctacggcat
    13561 aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac ttttttcatc tcatcaaggt
    13621 attacgtgcc aataagtatg accttatcgt caatctcacc gatcaatgga tggttgctat
    13681 actggttcgc ttattaaatg cccgtgtgaa aatttcccag gattatcatc atcggcagtc
    13741 tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg cagggtggaa atgtggtgga
    13801 aagtaactta tccgtgctga ccccattgcg agttgattcg ttggtgaagc agacaaccat
    13861 gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa cttgatcatg ctggtgttgg
    13921 acaaaattat gtggttatcc aacctacggc gcggcaaatc ttcaaatgct gggacaacgc
    13981 caagttttcc gctgtgattg atgccttaca tgctcgtggt tatgaagttg ttctgacgtc
    14041 cggcccagat aaagacgatc tggcctgcgt caatgaaatt gcgcagggat gccagacgcc
    14101 accagtaacg gcgctggctg gaaaggtgac cttcccggaa cttggtgcgt taatcgatca
    14161 tgcgcagctg tttattggcg ttgattccgc accggcgcat attgccgctg cagttaatac
    14221 gccgctgata tcgctgtttg gtgcgacaga ccatattttc tggcgtccct ggtcaaataa
    14281 catgattcaa ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc agcgtgaccg
    14341 aaatgagatg tatctttcgg ttattccggc ggcagatgtc attgctgctg tcgataaatt
    14401 actgccctcc tccacgacag gtacgtcgtt atgatcgttg ctttttgttt atataaatat
    14461 tttccctttg gcggtttgca gcgcgatttt atgcgtattg ctcagacagt cgccgcccga
    14521 ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg aataccctga tgtatttgaa
    14581 ctgatcaaag tgccggttaa atcgcatacc aatcacgggc gcaatgcgga gtattttgcc
    14641 tgggtgcaaa aacatttacg cgaacatccc gtcgataaag tcgttggatt caacaaaatg
    14701 ccggggctgg acgtttatta tgccgctgat gtttgttatg ccgagaaagt cgcgcaggaa
    14761 aaaggctttt tctatcacct gacgtcacgt tatcgccatt atgccgcctt tgagcgggca
    14821 accttcgaac agggcaagcc gacacagctg ctgatgctga cagataagca aatcgccgat
    14881 ttccagaaac attatcagac tgaagcggag cgttttcata ttctgccacc ggggatttat
    14941 cctgatcgta aatatagcca gcagccagct aatagccgtg aaatcttccg taagaagaat
    15001 ggaataaccg aacaacaata tttattgttg caggtcggtt cagacttcac gcgtaaaggt
    15061 gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt cgctgcgcca caacacattg
    15121 ctatatgttg ttggacagga taaaccgcga aaatttgagg cgctggcaga aaaacgcggc
    15181 gtgcgcagta atgttcactt cttctcgggg cgcaacgatg tctcggaatt aatggcggcg
    15241 gcggatttat tactgcatcc tgcctaccag gaagcagcgg gaattgtgct actggaagcg
    15301 attactgcag gattaccggt actaacaaca gccgtttgtg gctatgcgca ttatattgtc
    15361 gacgccaatt gcggcgaggc tattgctgag ccattccgcc aggaaacatt gaatgagatt
    15421 ttacgcaaag cgttaacgca atcttcattg cgccaggcct gggcggaaaa tgcgcgacat
    15481 tatgctgata cacaagattt atacagtctg ccagagaaag cggcggatat cataacgggt
    15541 ggtctggatg gttgaactta aagagccgtt tgccacgtta tggcgcggta aagatccttt
    15601 tgaggaagtt aaaaccttgc agggtgaggt ttttcgtgaa ctggaaactc gccgcactct
    15661 gcgatttgaa atggcgggca aaagctattt tctcaaatgg catcgcggca cgaccctgaa
    15721 agagataatc aaaaatttac tctcattgcg gatgccagta ttaggcgcag accgcgaatg
    15781 gagtgcgatt catcgactgc gggatgtcgg cgttgatact atgtatgggg tggcatttgg
    15841 cgaaaaaggt attaatccgc tgagcagaac ctcgtttatt attaccgaag atctgacccc
    15901 aaccatcagt ctggaagatt actgtgctga ctgggcgacc aacccaccag atgttcgcgt
    15961 aaagcgtatg cttattaagc gtgtcgcgac gatggtgcgc gatatgcatg ctgcgggcat
    16021 taaccaccgt gactgttata tctgtcattt cctgctgcac ttgccttttt ccggtaagga
    16081 agaggagtta aaaatttcgg taattgacct acaccgggcg cagcttcgca cgcgcgttcc
    16141 acgtcgttgg cgcgataaag atcttatagg gctttatttt tcttcaatga atattggcct
    16201 gactcagcgg gatatctggc ggtttatgaa agtgtatttt gccgccccgc ttaaagacat
    16261 tctcaagcag gaacaaggac tgctgtcgca agcagaagca aaagccacaa aaatcaggga
    16321 aagaacgatt cgaaaatcgt tgtaatttcc ggagagaaat atgagtgccc actattttaa
    16381 tccacaagag atgatcaata agacaatcat cttcgatgaa aggccagcgg cgtcagtggc
    16441 atcatcattc catgttgctt atggcattga taaaaacttt ctttttggtt gtggtgtttc
    16501 aatcacgtca gttttgttac ataacaacga cgtgagtttt gttttccacg tttttattga
    16561 tgatatccct gaagccgata tccagcgttt agcccaattg gcgaaaagct atcgtacctg
    16621 tatccagatc catctagtaa attgtgaacg gcttaaggca ttaccgacga ccaaaaattg
    16681 gtctattgcc atgtatttcc gttttgtaat tgcagattac tttattgatc aacaagataa
    16741 gatcctttac ctggatgctg atatcgcctg tcagggaaac ttaaagccgc tgataacaat
    16801 ggatcttgcc aataacgttg ctgctgttgt tactgaacgc gatgctaact ggtggtcgtt
    16861 acggggtcaa agtctgcagt gtaatgaact tgaaaagggc tactttaatt caggtgtcct
    16921 gttaattaat acactagcgt gggcgcagga gtccgtttct gctaaagcga tgtcgatgct
    16981 tgctgataaa gccatcgttt cccgtttcac ctatatggat caagatatcc ttaatcttat
    17041 cctgttaggg aaagttaaat tcattgatgc taaatacaat acgcaattta gtttaaatta
    17101 tgaattaaaa aaatcatttg tttgtccaat taatgatgaa accgtattaa ttcattatgt
    17161 cggcccgaca aaaccctggc attactgggc cggttatcca agtgcgcaac cttttatcaa
    17221 agccaaagaa gcatcgccct ggaaaaatga accgttaatg cggccagtta actcaaacta
    17281 tgctcgttat tgcgccaagc ataattttaa acaaaataaa ccaattaacg ggataatgaa
    17341 ttatatttat tatttttatt taaagataat aaaatgaaat tgagaaaagt taaatgaatg
    17401 aatttataaa agaacggttt tcgtatttag cagataataa aaaagaaaac gccccagagc
    17461 taaatgtttc ctacggtatc gataagaatt ttttgtatgg tgctggcgtt tcaatttctt
    17521 ccgttttgat taataattca gatattaatt ttgtctttca tgttttcact gattatgtgg
    17581 atgatgatta tttaaagtca tttaatgaaa cagcaaaaca atttaatacc tcaattattg
    17641 tatatttaat tgaccccaaa tactttgctg atctgccgac gtcacagttt tggtcatacg
    17701 cgacatactt cagggtattg tcttttgaat atctgagtga aagtatttcc acactgctgt
    17761 atctggatgc cgatgttgtt tgtaaaggaa gcctgaaacc tctcacagaa attatattta
    17821 aagatgagtt tgctgcggtc attcctgaca atgatagtac tcaggcggca tgtgcaaaac
    17881 gcctcaacat tcccgaaatg aatggacgtt atttcaatgc aggcgttatc tatgtcaatc
    17941 ttaaaaaatg gcatgaagca aatttgacac cgtatttact caaactttta cgaggggaaa
    18001 ctaaatatgg ctctcttaaa tatttagatc aggatgcgtt gaatatcgca tttaatatga
    18061 ataatatcta ccttgcgaag gattttgata ctatttatac cctgaaaaac gaactttatg
    18121 atcgtagtca tcgaaagtat cagcaaacca ttaccgataa aacagtgttg attcactata
    18181 cagggataac taaaccatgg catagctggg ctggatatcc gtctgcatca tactttaata
    18241 tcgcgcgtga acaatctccc tggaagaaat atcctcttaa agaggcgcgg actgttgcag
    18301 aaatgcagaa acaatataag catctgtttg cccatggtga gtatataaaa ggcataactt
    18361 cattaattaa gtacaagctt aagaaataaa ttactcttat gattacaagt atacgttatc
    18421 gcggcttctc attttattac aaagataatg ataataaata taaagaaatc tttgatgaaa
    18481 tactggccta taattttaaa acagtaaaag tattatgtaa tattgatgat accaaagtat
    18541 cgttgattga tacgaaatat ggtcgctatg tttttaaagt ctttgctccg aaaacgaaaa
    18601 ggaatgaacg ttttttaaaa tcttttgtaa aaggtgacta ctatcagaat ttgatcgttg
    18661 aaaccgatcg tgtcagaagt gcaggtctta cttttcctaa tgatttttat tttctggctg
    18721 agcgtaaaat tttcaactat gccagtgttt ttatcatgct tatcgagtat gtcgagggcg
    18781 tagagctcaa tgatatgccg attattccag aaaatgttaa agcagaaatt aaggcttcga
    18841 tggaaaaatt gcatgcctta aatatgctat cgggcgaccc acaccggggt aactttatcg
    18901 ttagtaaaga tggtgtcagg ataattgacc tatccggtaa aagctgtacg gcagagcgta
    18961 aagctcgtga tcgtctggcc atggaacgtc acctgggtat tgccaacgaa ataaaagatt
    19021 atggctacta ttcagtaatc tacagaacca aactacgcaa atttattaaa aaattaaaag
    19081 gcaaagcgta aaccacacag tcaaaacgga accaacatgg atttattagc agagagtatt
    19141 actgaagtcg ctgtctctgg ggaaattgct aacaccgatc gtgtgttaaa tatcgcttac
    19201 ggtattgacc gtaacttttt atttggtgcg gcagtatcta tgcaatcagt tgttatgcat
    19261 aacccggacc ttgcggttaa gtttcatctc tttaccgact acattgatga agattatcta
    19321 caacgtgtta atgcttttac cagcaaaaat gctaacgttg aagtaataat ttataaagtc
    19381 tccaatgcct ttattgatat cttccccagc ctgaaacagt ggtcttatgc aacattcttc
    19441 cgtttagttg cgttccagta tttgagtgaa actattgaaa atctgttata tatcgatgct
    19501 gatgtcatct gtaaaggctc attagctgga ttgcttgata ttaattttga tgaggataag
    19561 ttcgcagctg ttattaaaga tgtgcctttt atgcaggaaa aaccagcgaa gcgtctggct
    19621 atagagggac ttccagggaa ttatttcaac gccggtgtag tatatctgca gcttgaagca
    19681 tgggcgaaaa atgattttat gaataaagcc attgctatgc tggcaagtga cccgcagcac
    19741 acgaagtata aatgccttga tcaggatatt ttaaatattc tgttctttgg tcattgtaat
    19801 tttattagcg gcgattatga ttgcttttat ggcattgact atgagttaaa aaataaaagc
    19861 gatgaagatt ataaaaagac cattaccgat gatactaagc tgattcatta tgttggcgta
    19921 acgaagccct ggaacgactg gacgaattat ccctgccaga agtattttaa tgaggcttat
    19981 caggcttctt gctggaatga tgtggcgttt attccagcca cgaatgaaaa gcagtatcaa
    20041 gtgaaatatc aacatgcaaa gaaaaatggt gatacgttta acgcttttat ttacttcatt
    20101 aaatttaaat taaataagta taaaagaaaa ctatttggat aataataaaa atcatggatg
    20161 tctgagttac aataataatt aaggatgagt actatcatat tttattagtt caggcaagag
    20221 aacacgatga gcaatgatta ccctttagta tctataataa taccgacgta taattcatct
    20281 gattacatta ctgaaactct aacgaaatta gaaaaacaaa cttacccaaa ttttgaaatt
    20341 gttattgtta atgatggttc taaagataac acatcaaacg ttttgagaga gtatgggtta
    20401 acccactctc gattaattat tgtcaataaa gaaaatggcg gtgtttcgtc tgccaggaat
    20461 acaggtatcc gcaaggcgca aggacagttt atatgtttta tggatgatga tgatgagata
    20521 gatcctaact atctgctgaa gatgtatacc agacaacatg agacgggagg ggatgcgatt
    20581 tattgtgggc tttatggcca ttatataaaa gatggtgtta cttactcacc tataaataca
    20641 gagtttaatg aaggatcttt acttttcgac tttttttata aaaaggttag attccatata
    20701 gggtgcttgt ttataagaaa acaacttctg gaagagaata atcttttttt tgatgaagat
    20761 ttacgactag gagaagatct tgattttatc tatcgactgc taattacatg cgatatgtat
    20821 gcggttccat attatatgta taagcataac tatagagaaa attccttaat gaactcatgt
    20881 agaaccatca ctcattatcg acatgagtca tttgcgcacg aaagaatcta ctcttctgtg
    20941 atgcagttat acaaaggtaa ccggaaagaa gaaattcata cattattgag tcaaaataga
    21001 gcttatcata aaactcgtta tttatggaat gttctactta atggtgattt taaactattg
    21061 aatcaattag ttgaaagcaa tgaaaaagaa ttaagagatt gtaatcttcc tggcaagaga
    21121 gataagagac gagcaaaaat attagcatca aaaaattata ttctctggag gatggtaaga
    21181 ctggtaaata gaaaaaagaa taaacgttag atcttgcatt aaataaccca tcctcgtagc
    21241 ataggttgaa attatatgtc gttttgttgg aatgaaatta attctggtat aaagtcttta
    21301 attcttatat tatgtatttt ttctttaatg actttgtctt tatgggatga tgttgcaaca
    21361 aagtttcttc atgcagctgg aattatatct gcattgtatt ttcttgcgac accaaaaaaa
    21421 acaataacta ataatcctac tttgttaatt ttcatctcat tatgtctttt gggtatcgta
    21481 aatatcatct ggtattcaca ttataaagtt tcaggctctg tttataccaa tgcatatcgt
    21541 ggcccaatgg aaactggaaa aattgccttg tgtagcgctt ttattttctt agttcttttt
    21601 gctaaaaatg agatgagaac aaaaataaaa tttgggaaac taattctgtt cgcatccctg
    21661 gcaacgcagt tacttttttt tgcgcatgcc atgtggcaac atttctattt aaacgtcgac
    21721 cgtgttgcat tatcagcttc ccacgctaca acagcaggct acatcatcct ttttccttct
    21781 ttactggcat caattctcat tttaaaatcc gactttagac ataaaacaac attatataca
    21841 attaacttca tgcttagctt atgtgctgtc atagtaactg agacgcgtgc agccatatta
    21901 gtgtttccat tctttgcgtt aatattaatc gtaatggata gttatattaa taagcgaatt
    21961 aattataagt tatattgttt tattgcgatt gcattattag caggtgtatt ttcttttaaa
    22021 gatacattgc ttatgagaat gaatgactta aataacgatt tagttaatta ttcgcatgat
    22081 aacaccagaa cttcagtcgg tgcccgtctg gcaatgtatg aagttggctt aaaaacatat
    22141 tctccaatag gacaatcact ggaaaaacgt gcagaaaaaa tacatgagct agaagaaaaa
    22201 gagcctagat tgagtggcgc tttacctttt gtagattctc atttgcataa cgatctcata
    22261 gatacgttat caacgcgtgg tattcctgga gttgtattaa caattttagc attttcagca
    22321 atactcatat atgccttaag aactgctaaa gaaccttata ttttaatctt gcttttttca
    22381 ctactggtag taggcctaag tgatgtaata ctcttttcta aaccggttcc gactgctgtg
    22441 tttgtcacca taatattgct ttgtgcttat tttaaagcac aatcagacca atatttatta
    22501 gataagtaat gaatatatga aataaaatca gcaaggaggc tgattttatt tcaaaattat
    22561 ctattaaggg gttgtaattt aaatcatagc agcgttttca gcgttttctt caatgaatcg
    22621 cttaacagca tttgctgtca attgagacaa ctcattcccc ggagccctac aaaccatttg
    22681 attcttccca tacccaccaa ttaatcccgg atcggttggt ccataaaccg tgatattggg
    22741 tctatccagt gccgccgtta aatggcttaa ccccgtatcc accgacacta caaatttagc
    22801 cccggccagc acgcgggcaa cgccttccag actcatcttc ggcaatactt caacataatc
    22861 aaatccttcc gccagtcgtt tcgcccgttc ttcctcatgc ggcgcgcccc acggaagttt
    22921 aatccgtatt cctgaatcag ccagtaaacc aatcaattct cgccagtgtt cttccggcca
    22981 gtgtttatca tcacgggtcg tcgcatgaag aaatacggca tattcgccag catctgtagg
    23041 caggttcgtc agaaaatgct gtgcgatagc ataatcgccc tgggtttgcg gtttgctata
    23101 gcccaaactt ttggcaaaca gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat
    23161 atgatgctta caattgtaaa acaggctggc taaaggttcg cgagcggttt gccagtccaa
    23221 tccatgcttt acgccatgcg ccagacgtgt caccagtgcc gcgcttttta ccagcccctg
    23281 agcgtcgata actgcgtcat agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc
    23341 agcttttatg ggggccgaga accaggcttt acgccagcga cgtattgcca caggaataac
    23401 tcgctcaacg gcagcgtgcc aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc
    23461 aaacttaatc cctgggattg cctgctgggc atcagtgagt gcgggcaacg tatggagaac
    23521 atcgcccatc gacgatgttt taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa
    23581 caatagcgtg ttgagttctt ccagtacgcg ctggggagta atgtcgatca agctctggtg
    23641 ataaccctcc gcagcgtcac ctttacgcac tttgtgatag ccggtaatca gacggatcac
    23701 gcgcgcttta tgggatagcg gcggtgtgaa gtccgggcta cttggaccat acagggcaac
    23761 cagcggacga ttgagcgccg ccgcaacgtg catcaggcca gaatcgttag tgacaatggc
    23821 tttacaggct gcaatcagga taaccgcttg atcaagctgt gtttcccccg ccaggttccg
    23881 acaccatgcc tgctgctcgg tattcaaagc ggcaagaatc tcattgcccg cttcatgatc
    23941 tttcgcagag ccaaacagaa ccacctgata accttcatca atcagctgct ttgccagttc
    24001 cgcatagtgg tagtgtggcc agcgttttgc cggaccaaac tccgcacccg ggcaaaagcc
    24061 aatcatcgga cgttctgatg aaagcgaaaa ttgattacag gtatatgatt tttcaccttc
    24121 gctcacctgc aactgcggcc ataacaatgg ctgcggcaga tcttgtgcgg tacgcataat
    24181 gcctttgtca taggccagcg cgacatagcg tttcaccatt agcggccagg cttctttatc
    24241 gagcacgcgt acatcgttga gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg
    24301 aggaataccc gcgaagaaag gcactaatgc agatttgaag gagttgggta agacgtaggc
    24361 gcggtcgtag cgcttttcac gcaggctatg acccagtttg cggcgttcgc cgatttccag
    24421 cgctccgtga ccgagaggca tagggatagc ttcgttaact tccggcatcc gcgataataa
    24481 tggacggcac catgccggtg ccatcacatc gattatcgcc tggggatagc gcgcctggag
    24541 cgtgcgatag agactttgcg acatcatcat gtcgccaacc caagacgggc cgatcaccag
    24601 tattttcatg cagagctctt atgcgtcgcg attcagccag gccatgtatt ccgttacacc
    24661 ttcagcaacg gttttgaacg gtttgtcgta acccgccgcg cgcagatttg tcagatctgc
    24721 ctgagtgaac gcctggtagc ggcctttcag cttatccggg aacggaatgt attcgatctg
    24781 gcctttcttg tgataagcca gagtagcatc tgctacggcc tggaaggatt ccgcacgacc
    24841 agtaccgagg ttgaagatac cggaaacgcc attttccagg aaccacaggt ttacatctgc
    24901 cacgtcgccc acatagacga agtcacgttt gaagttctcg ctaccttcaa acagcttcgg
    24961 tgattcaccg ttgttaagct gagtgttgag atggaaagcg acgctcgcca tgctgccttt
    25021 atggccttca cgcggtccat aaacgttgaa atagcggaag ccaacaatct gcgagttcgc
    25081 ttcaggcagg atttggcgaa catattcatc aaacaggaat tttgagtaac cgtagacgtt
    25141 caacggtttt tcgtactcgc gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc
    25201 tgcggaagaa gcgtacagga acgggatttc gcgctccagg cagtagtgca gcagctcctt
    25261 ggagtattga tagttgttat ccatcatata cttgccgtcc cactcggtgg tggaagagca
    25321 tgcgccttcg tggaaaatcg cttcgacatc gccgaactct tcgccagcca taatctggat
    25381 caggaagtct tccttatcca tatagtccgc gatatccaga tccaccaggt tcacaaactt
    25441 ggtgccgtct ttcaggttgt ccaccaccag aatatcggtg atgcctttat cattcagggc
    25501 cttaacgatg ttgctgccga taaagcccgc gccgccggta acgatgatca taactgtaac
    25561 cttcgaatta tggagtcaga gacaatctca gacacgaatg cttctatcat atcactatac
    25621 ggcgttggct tcagccattc gccaaaagtg agattgatgg acgttattta tcctgaattt
    25681 tgcagaagtg ttaacgcgtc atctcgtcgc gacgtataag tttgggtaat atgtgctgga
    25741 atttgccctg tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat
    25801 ctggaaaccg cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag
    25861 caagcagata tcactgtggc tgatggaagc cacgtcatta acttttgtgc caacaactat
    25921 ctcgggctgg cgaatcatcc ggatctgatt gcggcagcaa aggcgggaat ggattctcac
    25981 ggtttcggca tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt
    26041 gaacaaaaac tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt
    26101 gatgctaacg gtggactgtt tgaaacgctg ctgggtgcgg aagacgccat tatctccgac
    26161 gcactgaacc acgcgtctat tattgatggt gtgcgtctgt gcaaagctaa acgctatcgc
    26221 tatgccaaca acgatatgca ggagctggaa gcacgtctga aagaagcgcg tgaagccggt
    26281 gcgcgtcatg tgctgattgc caccgatggt gtgttctcaa tggacggcgt gattgccaac
    26341 ctgaaaggcg tttgcgatct ggcagataaa tacgatgcct tggtgatggt ggatgactcc
    26401 cacgcggtcg gttttgtcgg tgaaaatggt cgtggttccc atgaatactg cgatgtgatg
    26461 ggccgggtcg atattatcac cggtacgctt ggtaaagcgc tgggcggggc ttctggtggt
    26521 tataccgcgg cgcgcaaaga ggtggttgag tggctgcgcc agcgttctcg tccgtacctg
    26581 ttctccaact cgctggcacc ggccattgtt gccgcgtcca tcaaagtact ggagatggtc
    26641 gaagcgggca gcgaactgcg tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa
    26701 atgtcggcgg cgggctttac cctggcggga gccgatcacg ccattattcc ggtcatgctt
    26761 ggtgatgcgg tagtggcgca gaaatttgcc cgagagctgc aaaaagaggg catttacgtc
    26821 accggtttct tctatccggt cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct
    26881 gcggcgcata cccctgagca aattacgcgt gcagtagaag cattcacgcg tattggtaaa
    26941 caactgggcg ttatcgcctg aggatgtgag atgaaagcgt tatccaaact gaaagcggaa
    27001 gagggcatct ggatgaccga cgtccctgta ccggaactcg ggcataacga tctgctgatt
    27061 aaaatccgta aaacagccat ctgcgggact gacgttcaca tctataactg ggatgagtgg
    27121 tcgcaaaaaa ccatcccggt gccgatggtc gtaggccatg aatatgtcgg tgaagtggta
    27181 ggtattggtc aggaagtgaa aggcttcaaa atcggcgatc gcgtttctgg cgaaggccat
    27241 atcacctgcg gtcactgtcg caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg
    27301 atcggcgtcg gcgttaaccg tccgggctgc ttcgccgaat atctggtgat cccggcgttc
    27361 aacgccttca aaatcccaga caatatctcc gacgacctgg cttccatttt tgatcccttc
    27421 ggtaacgccg tgcataccgc gctgtcgttc gatctggttg gcgaggatgt gctggtctct
    27481 ggtgcaggtc cgataggtat tatggctgcg gcggtggcga aacacgttgg tgcacgcaat
    27541 gtggtgatca ctgatgttaa cgaataccgc cttgagctgg cgcgcaaaat gggtatcacc
    27601 cgtgcggtta acgtcgcgaa agaaaacctt aatgatgtga tggctgaact gggcatgacc
    27661 gaaggctttg atgtcggtct ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctt
    27721 gacaccatga accacggcgg ccgtattgcg atgctgggta ttccgccgtc tgatatgtct
    27781 atcgactgga ccaaagtgat ctttaaaggc ttgttcatta aaggtattta cggtcgtgag
    27841 atgtttgaaa cctggtacaa gatggcggcg ctgattcagt ctggcctcga tctctcgccg
    27901 atcattaccc atcgtttctc tatcgatgat ttccagaagg gctttgacgc tatgcgttcg
    27961 ggccagtccg ggaaagtaat tctgagctgg gattaacacg aacaagggct ggtattccag
    28021 cccttttatc tggggataat ctgttgaata tgtaaaatcc tgtcagtgta ataaagagtt
    28081 cgtaattgcg ctgatctctt atatagctgt tctcattatc tctctaccct gaagtgactc
    28141 tctcacctgt aaaaataata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa
    28201 acgtcaggat aacttcagag gtcgtcggta atttatgatg aacagcacca ataaacttag
    28261 tgttattatt ccgttatata atgcgggcga tgatttccgc acttgtatgg aatctttaat
    28321 tacgcaaacc tagactgctc tggaaatcat tattattaac gatggttcaa cggataattc
    28381 tgttgaaata gcaaagcatt acgcagaaaa ctatccgcac gttcgtttgt tgcatcaggc
    28441 gaatgctggc gcatcggtag cgcgtaatcg tgggatcgag gtggcgacgg gcaaatatgt
    28501 cgcttttgtc gatgctgacg atgaggtcta tcccaccatg tacgaaacgc tgatgaccat
    28561 ggcgttagag gacgacctcg acgtggcgca gtgcaacgct gactggtctt ttcgtgaaac
    28621 gggagaaacc tggcaatcca tccccagcga tcgccttcgc tcaaccggcg tattaaccgg
    28681 cccggactgg ctgcggatgg ggctttcttc gcgccgttgg actcacgttg tctggatggg
    28741 ggtttatcgc cgtgatgtta ttgttaaaaa taacattaaa tttattgccg gattacatca
    28801 tcaggatatt gtctggacaa cagaattcat gtttaacgcg ctgcgtgcgc gatataccga
    28861 gcaatcatta tataaatatt atctgcataa tacgtcagtg agtcggttgc atagacaagg
    28921 gaataaaaac cttaattatc aacgtcacta tattaagatt acccgcctgc tggagaaatt
    28981 aaatcgaaat tatgccgaca aaattacgat ttatccggaa tttcatcagc aaataactta
    29041 cgaagcattg cgtgtttgcc atgcggtgcg caaagagccg gatattctta cccgccaacg
    29101 gatgattgcc gagatattta cttccggtat gtataagcgc ctgattacca atgtgcgcag
    29161 cgtgaaggtc ggttaccagg cgttactgtg gtctttccgc ttatggcaat ggcgcgacaa
    29221 aacgcggtcg caccatcgca ttacgcgtag cgcctttaat ttgcgctagc gttaaacttc
    29281 gccgcttcgg gctgtttgcc ccagccttgc cactgatgct ggaagtaaac gatcagcgtg
    29341 ctctggctga tgctttcgct taatacttca aagaagcgat tagcataaac aggttctatc
    29401 ggtttcttcg gtttgcacag cctcacgcca cggaacggat tacgcggtgc atcaggcaca
    29461 gcgtttttcg gcggtgtaga agtatcaacc tgcggttcat tcagcaagct gctggctttc
    29521 accagcgtaa tgtctggcgg caggttataa accatctgtt gtagcacgcg taccgttaaa
    29581 gggtgaggat gcccaatggc aatggttgaa ccgttgcgac gcgccagatc aattgcgcga
    29641 ttaaattgca cacggatgtc cgcttcgttc tgcgaatcgt cgaggaacac cttccgttta
    29701 ataaccttca cgccagtacc ttgcgcggcg cgcatcgcct gggtattacc gatggttacg
    29761 ctgtcgagga agtaaagatt gtaacgctcc agtgcctgca tcactttctg cataccaaac
    29821 aggttagagg tcatcttgct acccatgtgg ttgttgatcc ccacggcata gggcacgtta
    29881 ttgaccgcac tacgaataat gcgctcaatt tcgtcgctgc tcatctccgg gcgcagcgta
    29941 tttttctcca gcggctgttt actcagtggt gccatcggga gatgaatcaa cacttcgtgc
    30001 ccgctgttat gcgctttggt cgccatctct ctggcgtgcg gtgaatcggg taatacagcg
    30061 acggagatag cggagggcat cgccagcacc tggttttcgt tgtgcgggcg atacccaaaa
    30121 tcatcaatga cgatggcaag tttgccagca agtacggggg aggagagcgc caacagagcg
    30181 gcaaatgcaa gaacgttacg acgaaatgga aacaaaactt atcttcccaa ccacggctgt
    30241 ggattgaccg cctgaccctg gcggcgaatt tcgaaataga gtgaaggccg accctgaccg
    30301 ccactgctgc ccaccagtgc aattggctgg cccgcgcgaa cctgcgaacc aacgctcacc
    30361 agtgcgctct gattatagcc gtaaagactc atgtcgcctt taccgtgctc aaccaccacc
    30421 accagaccgt aaccttgcag ccagtcagcc agaatcactc gaccgtcggc aattgcttta
    30481 acttcagtac cttcagaggc accgataacc atccctttcc agcgtaattc accctgtagc
    30541 tgctcgccat agcgatgcag cgtcggcccg cgaaccggcc agaatgcctg accacgcggc
    30601 gcacccagac caccagttcg ggacatcagc gatttttcgc tttcggtcgg tttgtaggtg
    30661 gtgcctttgc gcgtcgcttc tttctggcgg tcgcgaaccg cctgggcctc acgcgcttct
    30721 cgttcagcac gcgctttcgc cgcggcttcc gcacgggcaa tgctgttacg cagacgggat
    30781 tcgttggcac gcagttcact tagctgctgc tgaccttgct ggatggaaga ttccagcccc
    30841 gccagcgtct ttttacgctc gttcagagcc tgagtcagtt tcgcctgttg ggcgcgctgt
    30901 tcatataaca gcgtttgttg ctcgctttgt ttctcttcca gttcagcacg ctgcatggcg
    30961 acttcttcac gcgtttgctt caactgggca atggtttctt gtcgcgcctg gttgagatag
    31021 ccgaaataag cctgtaaacg ctgtccacac tggctttctt caccgctgag aattagctga
    31081 ataccggtat gctcgccctg acggaatgcg gcatccagtt gtgcggcgag gctgcgctcc
    31141 tgggcggctt tttgctgctc cagtttggca atcgacgcgt tcatctcatc aatctgttta
    31201 ttgagttgat tgagcgtgtt ttgcgtttcg cgcagcttac gggtggcttc agagatcgct
    31261 tcttcctgct ttttcaattg tgcgagcagg cttgcgcgtt gttgttgctt ttggcgtacc
    31321 gcgcgctctt ttgcggcgat atcggcctga atagatttga gttggtcacg ctcatccgcg
    31381 tgggcggaaa aggcgcacaa caatacgcca gcgctaagaa cgctggcgta gatgatgggc
    31441 ctgattgcaa acctgcgcgg tttcacggcc cgtgtcatgg tattaatcgc ctttcccctc
    31501 atggggaggg attattccac gatgaacagc ggcttaccag tcatctcttg cgggatttcc
    31561 atacccatca gcgacaacat ggtcggcgcg atgtcagaaa gtttgccgcc tgcaaccgct
    31621 ttcacgttct tatcaccaac gtaaatcagc ggaactggca ggttggtgtg tgccgtgtgt
    31681 gcctgacccg ttgccggatc gcgcatctgt tcagcgttac cgtggtcagc ggtgatcagc
    31741 agttgtccac ccacggactc aaccgctttc gcgacttctt ccacgcagtg atccagtgct
    31801 tcaaccgctt taaccgccgc ttccatcacc ccggtgtgac ctaccatgtc gccgttcgga
    31861 tagttacaga taatggtgtc gtatttgccg ctcttgatgg ccgcaaccag tttttcggtc
    31921 agctctgcgg agctcatttc cggttgcaga tcgtaggtag ccactttcgg tgagttgatc
    31981 agaatgcgat cttcgccttt gaatgactct tctacgccac cgttgaagaa gaaagtaacg
    32041 tgggcatatt tttcggtttc ggaaatacgc aactgagttt tgtcgttttt cgccatccac
    32101 tcgccgaagg tgttaaccag ggaagcgggt gggtaagcaa ccgcagtttt gatgtcagcg
    32161 gcgtattcgg tcagcatcac gaaatcgacg ttaaccactt tcttacgcgc gaagccgtcg
    32221 aaatctgcgt ttacgaatgc acgagtgatt tcgcgcgcgc ggtcagcacg gaagttcatg
    32281 aaaatcagcg catcgccgtc ttccattgcc gcatctggct gaccttctgc acgaataacg
    32341 gtcgctttca cgaactcgtc gttttcgtcg cgagcataag ctgcctgcaa accagcaacg
    32401 gcagtatcgg cctggaactc accctgcgcc agagtcagca ggtcataggc tttttcaacg
    32461 cgatcccaac ggttatcgcg gtccatcgcg tagtaacgac caatgatgga cgctacgcgg
    32521 cctttaccca gcgcagcaaa tttctcttca aattttttca gcgaggattc agcactgcgc
    32581 ggcggggtgt cgcgaccgtc aaggaatgcg tgcaggtaga ttttttctgc gccgcgttca
    32641 gctgccagtt ctaccatcgc catgatgtga tcttcgtggc tgtgtactcc gcctgcggag
    32701 agcagaccca taatgtgtac tgctttacct gcgtttttcg ctttatctac cgcgccagtc
    32761 agcactggat tagcaaagaa agcgcgatct ttgatttcaa cgtccagacg agtcaggtcc
    32821 tgatacacga tgcggccggc acccaggtta acgtggccta cttcggagtt acccatctga
    32881 cggtcaggca gaccgacttc cagaccggaa gcgtcgatca gggtatgcgg acgattggcc
    32941 cacagtgcat ccattaccgg ggttttagca ctaaaaatgg cgttatcctg ctgttcttca
    33001 cgatagccat agccatccag aatcaccagt accataggtt ttttagaaac cgacattgcg
    33061 acaacctcat actcaagagt caaaatttgc gtaattttac tacagctgaa tcgataaaat
    33121 agcctctgaa gatcaaaata tacgaccgct cgcggcaagc cgatgccaga tttgcgcttt
    33181 tttttcgtcg tgccccgcaa aattgcattc cagttaacgc gctggctgta tttgccgcac
    33241 cgcgcaggta tactcctttc ctggtttttt taatcactac gtcgggagtt gttacccccc
    33301 atgcaagaaa ttatgcaatt tgttggccgt catcccatac tgagtatcgc ttggatcgcg
    33361 ttactggtgg cggttcttgt gactacgttt aagagcctga cctcgaaagt gaaggtgatt
    33421 actcgtggtg aagctacgcg tctgatcaac aaagaagacg ctgtggttgt ggatttacgt
    33481 cagcgtgatg acttccgtaa aggccatatc gcaggttcta tcaatctgtt gccgagcgaa
    33541 atcaaagcca acaatgttgg tgagcttgag aagcacaaag acaaaccggt tatcgtggta
    33601 gacggttctg gcatgcagtg ccaggagcct gcaaacgcgc tgacgaaagc tggttttgcg
    33661 caagtattcg tgctgaaaga gggcgttgct ggctgggctg gcgaaaactt gcctctggtg
    33721 cgcggcaaat aatttacctg tcgtctttca tgatgcaggc cgttagctgc aattaggccc
    33781 ccagcgactt attgttataa gcttctgggg acactccatt ttgcagccgc cctgcaacat
    33841 gaaatttcag ggtaaaaaaa caggagctaa attatggcca atgttgaaat ctataccaaa
    33901 gaaacctgcc cgtattgcca tcgtgcaaaa gcactgctga gcagcaaggg cgtgagtttt
    33961 caggagttgc cgatcgatgg caatgccgcc aagcgtgaag agatgatcaa acgcagcggt
    34021 cgcaccacgg ttccccagat ttttattgac gcacagcaca ttggcggctg tgatgacttg
    34081 tatgcattgg atgcacgtgg tggactggat cccctgctga aataacgtgt gaacgttggc
    34141 attacattgc gcagtattta aggacaacac ttaagggttt tctacacatg tcagaacaaa
    34201 acaacactga aatgactttc cagatccaac gtatttatac caaggatatc tctttcgaag
    34261 cgccgaacgc gccgcacgtt ttccagaaag attggcaacc agaagttaaa cttgatctgg
    34321 atacggcatc ttcccaactg gcagatgacg tatacgaagt ggtactgcgt gttaccgtaa
    34381 cggcctccct gggcgaagaa accgcgttcc tgtgtgaagt tcagcagggc ggtattttct
    34441 ccatcgcggg tatcgaaggc acccagatgg cacattgcct gggcgcatac tgcccgaaca
    34501 ttctgttccc gtatgctcgt gagtgcatca ccagcatggt atcccgcggt acattcccgc
    34561 aactgaacct tgcgccggtt aacttcgatg cgctgttcat gaactatttg cagcagcagg
    34621 ctggcgaagg tactgaagaa catcaggatg cctgatgaac caacgtaatg cttcaatgac
    34681 tgtgatcggt gccggctcgt acggcaccgc tcttgccatt accctggcaa gaaatggcca
    34741 cgaggttgtc ctctggggcc atgaccctga acatatcgca acgcttgaac gcgaccgctg
    34801 taacgccgcg tttctccccg atgtgccttt tcccgatacg ctccatcttg aaagcgatct
    34861 cgccactgcg ctggcagcca gccgtaatat tctcgtcgtc gtacccagcc atgtctttgg
    34921 tgaagtgctg cgccagatta aaccgctgat gcgtcctgat gcgcgtctgg tgtgggcgac
    34981 caaagggctg gaagcggaaa ccggacgtct gttacaggac gtggcgcgtg aggccttagg
    35041 cgatcaaatt ccgctggcgg ttatctctgg cccaacgttt gcgaaagaac tggcggcagg
    35101 tttaccgacg gctatttcgt tggcctctac cgaccagacc tttgccgatg atctccaaca
    35161 attgctgcac tgtggcaaaa gtttccgcgt ttacagcaac ccggatttca tcggtgtgca
    35221 gcttggcggt gcggtgaaaa acgtcattgc cattggcgcg gggatgtccg acggtatcgg
    35281 ttttggtgcg aatgcgcgta cggcactgat cacccgtggg ctggctgaaa tgtcgcgtct
    35341 tggcgcggcg ctgggtgccg agcctgccac ctttatgggc atggcggggc tgggcgatct
    35401 tgtgcttacc tgtaccgaca accagtcgcg taaccgccgt tttggcatga tgctcggtca
    35461 gggcatggat gtacaaagcg cgcaggagaa gattggtcag gtggtggaag gctaccgcaa
    35521 tacgaaagaa gtccgcgaac tggcgcatcg cttcggcgta gaaatgccaa taaccgagga
    35581 aatttatcaa gtattatatt gcggaaaaaa cgcgcgcgag gcagcattga cgttattagg
    35641 tcgtgcacgc aaggacgagc gcagcagcca ctaaccccag ggaacctttg ttaccgctat
    35701 gacccggccc gcgcagaacg ggccggtcat tatctcatcg tgtggagtaa gtaatgtcgt
    35761 gtgaagaact ggaaattgtc tggaacaata ttaaagccga agccagaacg ctggcggact
    35821 gtgagccaat gctggccagt ttttaccacg cgacgctact caagcacgaa aaccttggta
    35881 gtgcactgag ctacatgctg gcgaacaagc tgtcatcgcc aattatgcct gctattgcta
    35941 tccgtgaagt ggtggaagaa gcctacgccg ctgacccgga aatgatcgcc tctgcggcct
    36001 gtgatattca ggcggtgcgt acccgcgacc cggcagtcga taaatactca accccgttgt
    36061 tatatctgaa gggttttcat gccttgcagg cctatcgcat cggtcactgg ttgtggaatc
    36121 aggggcgtcg cgcattggca atctttctgc aaaaccaggt ttctgtgacg ttccaggtcg
    36181 atattcaccc ggcagcaaaa attggccgtg gcattatgct cgaccacgcc acgggcatcg
    36241 ttgtgggtga aacggcggta attgaaaacg acgtatcgat tctgcaatct gtgacgcttg
    36301 gcggtacggg taaatctggt ggtgaccgtc acccgaaaat tcgtgaaggt gtgatgattg
    36361 gcgcgggcgc gaaaatcctc ggcaatattg aagttggtcg cggcgcgaag attggcgcag
    36421 gttccgtggt gctgcaaccg gtgccgccgc atacaaccgc cgctggcgtt ccggctcgta
    36481 ttgtcggtaa accagacagc gataagccat caatggatat ggaccagcat ttcaacggta
    36541 ttaaccatac atttgagtat ggggatggga tctaatgtcc tgtgatcgtg ccggatgcga
    36601 tgtaatcatc tatccggcat acagtaacta atctctcaac aacgctcccg gatatcccag
    36661 ctgccgccag gcttcataca ccactaccga caccgcattg gacagattca tgctgcggct
    36721 gtccggcacc atcggaatgc gaattttttg ttcagcgggc agggcatcaa gaatgctcgc
    36781 tggcaggccg cgtgtttccg ggccgaacat cagataatcg ccatcctgat agcttacggc
    36841 gctgtgagca ggcgtacctt tcgtggtgag ggcaaacagg cgctggggat tttctgcttc
    36901 gaggaacgcg cgatagtcat gatgacgcgt aacggcggta aactcgtgat agtccagccc
    36961 cgcgcggcgc aggcgcttat cgtcccaggc aaatcccatc ggttcgatga tatgcagacg
    37021 aaagccggta ttagcgcaaa gacggatgat gttgccagta tttggcggaa tttctggttc
    37081 gtaaagtacg atgtttagca tactgccccc ttagtgcggg ggcaggatag cagaaaattg
    37141 gtgaaccgtg cggccttttc ccctcacccc ggccctcccc ctgtctcttg atcaggagat
    37201 ctgatcaaga gacagctccc tgagggagag ggtt
//
LOCUS       DADLTD010000013        36824 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLTD010000013 DADLTD010000000
VERSION     DADLTD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.013"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            583..669
                     /gene="leuL"
                     /locus_tag="JN701_000520"
     CDS             583..669
                     /gene="leuL"
                     /locus_tag="JN701_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY7363062.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            762..2333
                     /gene="leuA"
                     /locus_tag="JN701_000521"
     CDS             762..2333
                     /gene="leuA"
                     /locus_tag="JN701_000521"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY7363063.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2333..3424
                     /gene="leuB"
                     /locus_tag="JN701_000522"
     CDS             2333..3424
                     /gene="leuB"
                     /locus_tag="JN701_000522"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY7363064.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3427..4827
                     /gene="leuC"
                     /locus_tag="JN701_000523"
     CDS             3427..4827
                     /gene="leuC"
                     /locus_tag="JN701_000523"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY7363065.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4838..5443
                     /gene="leuD"
                     /locus_tag="JN701_000524"
     CDS             4838..5443
                     /gene="leuD"
                     /locus_tag="JN701_000524"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY7363066.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5492..6574)
                     /locus_tag="JN701_000525"
     CDS             complement(5492..6574)
                     /locus_tag="JN701_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7363067.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6676..6807)
                     /gene="sgrT"
                     /locus_tag="JN701_000526"
     CDS             complement(6676..6807)
                     /gene="sgrT"
                     /locus_tag="JN701_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY7363068.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6896..8551
                     /locus_tag="JN701_000527"
     CDS             6896..8551
                     /locus_tag="JN701_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY7363069.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8585..8684
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8715..9698
                     /gene="thiB"
                     /locus_tag="JN701_000528"
     CDS             8715..9698
                     /gene="thiB"
                     /locus_tag="JN701_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY7363070.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9674..11284
                     /gene="thiP"
                     /locus_tag="JN701_000529"
     CDS             9674..11284
                     /gene="thiP"
                     /locus_tag="JN701_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY7363071.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11268..11966
                     /gene="thiQ"
                     /locus_tag="JN701_000530"
     CDS             11268..11966
                     /gene="thiQ"
                     /locus_tag="JN701_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY7363072.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(12079..12843)
                     /locus_tag="JN701_000531"
     CDS             complement(12079..12843)
                     /locus_tag="JN701_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7363073.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12929..13807)
                     /gene="araC"
                     /locus_tag="JN701_000532"
     CDS             complement(12929..13807)
                     /gene="araC"
                     /locus_tag="JN701_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY7363074.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14146..15846
                     /gene="araB"
                     /locus_tag="JN701_000533"
     CDS             14146..15846
                     /gene="araB"
                     /locus_tag="JN701_000533"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY7363075.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15857..17359
                     /gene="araA"
                     /locus_tag="JN701_000534"
     CDS             15857..17359
                     /gene="araA"
                     /locus_tag="JN701_000534"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY7363076.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17559..18254
                     /gene="araD"
                     /locus_tag="JN701_000535"
     CDS             17559..18254
                     /gene="araD"
                     /locus_tag="JN701_000535"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7363077.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18329..20680
                     /gene="polB"
                     /locus_tag="JN701_000536"
     CDS             18329..20680
                     /gene="polB"
                     /locus_tag="JN701_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY7363078.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20844..23750
                     /gene="rapA"
                     /locus_tag="JN701_000537"
     CDS             20844..23750
                     /gene="rapA"
                     /locus_tag="JN701_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY7363079.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23762..24421
                     /gene="rluA"
                     /locus_tag="JN701_000538"
     CDS             23762..24421
                     /gene="rluA"
                     /locus_tag="JN701_000538"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY7363080.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24538..25353)
                     /gene="djlA"
                     /locus_tag="JN701_000539"
     CDS             complement(24538..25353)
                     /gene="djlA"
                     /locus_tag="JN701_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY7363081.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25608..27962
                     /gene="lptD"
                     /locus_tag="JN701_000540"
     CDS             25608..27962
                     /gene="lptD"
                     /locus_tag="JN701_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY7363082.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28015..29301
                     /gene="surA"
                     /locus_tag="JN701_000541"
     CDS             28015..29301
                     /gene="surA"
                     /locus_tag="JN701_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY7363083.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29301..30290
                     /gene="pdxA"
                     /locus_tag="JN701_000542"
     CDS             29301..30290
                     /gene="pdxA"
                     /locus_tag="JN701_000542"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY7363084.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30287..31108
                     /gene="rsmA"
                     /locus_tag="JN701_000543"
     CDS             30287..31108
                     /gene="rsmA"
                     /locus_tag="JN701_000543"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY7363085.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            31111..31488
                     /gene="apaG"
                     /locus_tag="JN701_000544"
     CDS             31111..31488
                     /gene="apaG"
                     /locus_tag="JN701_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY7363086.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31495..32337
                     /gene="apaH"
                     /locus_tag="JN701_000545"
     CDS             31495..32337
                     /gene="apaH"
                     /locus_tag="JN701_000545"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY7363087.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVISSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32415..32894)
                     /gene="folA"
                     /locus_tag="JN701_000546"
     CDS             complement(32415..32894)
                     /gene="folA"
                     /locus_tag="JN701_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY7363088.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33086..34948)
                     /gene="kefC"
                     /locus_tag="JN701_000547"
     CDS             complement(33086..34948)
                     /gene="kefC"
                     /locus_tag="JN701_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY7363089.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34941..35471)
                     /gene="kefF"
                     /locus_tag="JN701_000548"
     CDS             complement(34941..35471)
                     /gene="kefF"
                     /locus_tag="JN701_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY7363090.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35553..>36824)
                     /locus_tag="JN701_000549"
     CDS             complement(35553..>36824)
                     /locus_tag="JN701_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005116114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7363091.1"
                     /translation="DGYVLVMIGVALEQLTPALKLDADWIGLLGAGTLAGLFVGTSLF
                     GYISDKVGRRKMFLIDIIAIGVISVATMFVSSPVELLVMRVLIGIVIGADYPIATSMI
                     TEFSSTRQRAFSISFIAAMWYVGATCADLVGYWLYDVEGGWRWMLGSAAIPCLLILIG
                     RFELPESPRWLLRKGRVKECEEMMIKLFGEPVAFDEEQPQQTRFRDLFNRRHFPFVLF
                     VAAIWTCQVIPMFAIYTFGPQIVGLLGLGVGKNAALGNVVISLFFMLGCIPPMLWLNT
                     AGRRPLLIGSFAMMTLALAVLGLIPDMGIWLVVMAFAVYAFFSGGPGNLQWLYPNELF
                     PTDIRASAVGVIMSLSRIGTIVSTWALPIFINNYGISNTMLMGAGISLFGLLISVAFA
                     PETSRDVIGANQQYDDPRAENGVNCSDFSLF"
BASE COUNT         8958 a        10081 c         9690 g         8095 t
ORIGIN      
        1 atgcatataa aataagaaaa agcaaaatga gtaaaattcg gaaaacataa agatacgggt
       61 tctgataaaa acagaatcca ttgccgtgga atgagtcatt taccacagca taataatcca
      121 taatgttttt gttgcgaaaa caatctaatc ataactaccg cgaatactca atcatctaca
      181 aaatggatta aatgtgctta tttaataatt aatttatata tttaatgcat taattattaa
      241 cattaatgga tcaataatat tcaccatatc aatatcaaaa aaaattcgca aaacatataa
      301 ttcaatacaa atcatcagga taggttttgc aacgcgtgca ttttgtcccc tttttcctcg
      361 ttgattagat gcaaaaattt attctgaaat atgtcaacct atgaaaagtg tcggtcgtta
      421 aacagaaatt aatatcgctt gctttaacca ccgcagcaca attagctaat tttacggatg
      481 cagaactcgc gctggcggga cgtttttatt gcgtcagggt tgacatccgt ttttgtatcc
      541 agtaactcta aaagcatatc gcattcatct ggagctgatt taatgactca catcgttcgc
      601 tttatcggtc tactactact aaacgcatct tctttgcgcg gtagacgagt gagcggcatc
      661 cagcattaag ccagcacgca gtcaaacaaa aaacccgcgc cattgcgcgg gtttttttat
      721 gcccgaagcg aggcgctcta aaagagacaa ggacccaaac catgagccag caagtcatta
      781 ttttcgatac cacattgcgc gacggtgaac aggcgttaca ggcaagcttg agtgtgaaag
      841 aaaaactgca aattgcgctg gcccttgagc gtatgggtgt tgacgtgatg gaagtcggtt
      901 tccccgtctc ttcgccgggc gattttgaat cagtgcaaac catcgcccgc caggttaaaa
      961 acagccgcgt atgcgcgtta gctcgctgcg tggagaaaga tatcgacgtg gcggccgaat
     1021 ccctgaaagt cgccgaagcc ttccgtattc atacctttat tgccacttcg ccaatgcaca
     1081 tcgccaccaa gctgcgcagc acgctggatg aagtgatcga acgcgctatc tatatggtga
     1141 aacgcgcccg taattacacc gatgatgttg aattttcttg cgaagatgcc gggcgtaccc
     1201 ccattgccga tctggcgcga gtggtcgaag cggcgattaa tgccggtgcc accaccatca
     1261 acattccgga caccgtgggc tacaccatgc cgtttgagtt cgccggaatc atcagcggtc
     1321 tgtatgaacg cgtacctaac atcgacaaag ccattatttc cgtacatacc cacgacgatt
     1381 taggcctggc ggtcggcaac tcactggcgg cggtacatgc cggtgcacgc caggtggaag
     1441 gtgcaatgaa cgggatcggc gagcgtgccg gtaactgttc gctggaagaa gtcatcatgg
     1501 cgattaaagt tcgtaaggat attctcaacg tccacaccgc cattaatcac caggagatat
     1561 ggcgcaccag ccagttagtt agccagattt gtaatatgcc gatcccggca aacaaagcca
     1621 ttgttggcag cggcgcattc gcccactcct ccggtatcca ccaggatggc gtgctgaaaa
     1681 accgcgaaaa ctacgaaatc atgacaccag aatctattgg tctgaaccaa atccagctga
     1741 atctgacctc tcgttcgggg cgtgcggcgg tgaaacatcg catggatgag atggggtata
     1801 aagaaagtga atataattta gacaatttgt acgatgcttt cctgaagctg gcggacaaaa
     1861 aaggtcaggt gtttgattac gatctggagg cgctggcctt catcggtaag cagcaagaag
     1921 agccggagca tttccgtctg gattacttca gcgtgcagtc tggctctaac gatatcgcca
     1981 ccgccgccgt caaactggcc tgtggcgaag aagtcaaagc agaagccgcc aacggtaacg
     2041 gtccggtcga tgccgtctat caggcaatta accgcatcac tgactataac gtcgaactgg
     2101 tgaaatacag cctgaccgcc aaaggtcacg gtaaagatgc gctgggtcag gtggatattg
     2161 tcgccaacta caacggtcgc cgcttccacg gcgtcggcct ggccaccgat attgtcgagt
     2221 cctccgccaa agccatggtg cacgtactga acaatatctg gcgtgccgca gaagtcgaaa
     2281 aagagttgca acgcaaagct caacacaacg aaaacaacaa ggaaaccgtg tgatgtcgaa
     2341 gaattaccat attgccgtat tgccggggga cggtattggt ccggaagtga tgacccaggc
     2401 gctgaaagtg ctggatgccg tgcgcaaccg ctttgcgatg cgcatcacca ccagccatta
     2461 cgatgtaggc ggcgcagcca ttgataacca cgggcaacca ctgccgcctg cgacggttga
     2521 aggttgtgag caagccgatg ccgtgctgtt tggctcggta ggcggtccga aatgggaaca
     2581 tttaccacca gaccagcaac cagaacgcgg cgcgctgttg cctttgcgta agcacttcaa
     2641 attattcagc aacctgcgtc cggcaaaact gtatcagggg ctggaagcat tctgtccgct
     2701 gcgtgctgac attgccgcta acggcttcga catcctgtgc gtgcgcgaac tgaccggcgg
     2761 catctatttc ggtcagccaa aaggccgcga aggtagcgga cagtatgaaa aagcgtttga
     2821 taccgaggtg tatcaccgtt ttgagatcga acgtatcgcc cgcatcgcgt ttgaatctgc
     2881 ccgcaagcgt cgccacaaag tcacctcaat cgacaaagcc aacgtgctgc aatcctctat
     2941 tttatggcgg gagatcgtta acgagatcgc cacggaatac ccggatgtcg aactggcgca
     3001 tatgtacatc gacaacgcca ccatgcagct gattaaagat ccatcacagt ttgacgtcct
     3061 gctgtgctcc aacctgtttg gcgacattct gtctgacgag tgcgcaatga tcactggctc
     3121 gatggggatg ttgccttccg ccagcctgaa cgagcaaggt tttggtctgt atgaaccggc
     3181 aggcggctca gcaccagata tcacaggcaa aaacatcgcc aacccgattg cgcaaattct
     3241 gtcgctggca ctgctgctgc gctacagcct ggatgccgat gatgcggctt gcgccattga
     3301 acgcgccatt aaccgcgcat tagaagaagg cattcgcacc ggggatttag cccgtggcgc
     3361 tgccgccgtt agtaccgatg aaatgggcga tatcattgcc cgctatgtgg cagaaggggt
     3421 gtaatcatgg ctaaaacgtt atacgaaaaa ttattcgacg ctcacgtagt gtacgaagcc
     3481 gaaaacgaaa ccccgctgtt atatatcgac cgccacctgg tgcatgaagt gacctcaccg
     3541 caggcgttcg atggtctgcg cgcccacggt cgcccggtac gtcagccggg caaaaccttc
     3601 gccactatgg atcacaacgt ctctacccag acgaaagaca ttaatgcctg tggtgaaatg
     3661 gcgcgcatcc agatgcagga gctgattaaa aactgcaaag aatttggcgt cgaactgtat
     3721 gacctgaatc acccgtatca ggggatcgtc cacgtaatgg ggccggaaca gggcgtcacc
     3781 ttgccgggga tgaccattgt ctgcggcgac tcgcataccg ccacccacgg cgcgtttggc
     3841 gcactggcct ttggtatcgg cacttccgaa gttgaacacg tactggcaac gcaaaccctg
     3901 aaacagggcc gcgcaaaaac catgaaaatt gaagtccagg gcaaagccgc gccgggcatt
     3961 accgcaaaag atatcgtgct ggcaattatc ggtaaaaccg gtagcgcagg cggcaccggg
     4021 catgtggtgg agttttgcgg cgaagcaatc cgtgatttaa gcatggaagg tcgtatgacc
     4081 ctgtgcaata tggcaatcga aatgggcgca aaagccggtc tggttgcacc ggacgaaacc
     4141 acctttaact atgtcaaagg ccgtttgcat gcgccgaaag gcaaagattt cgacgacgcc
     4201 gttgcctact ggaaaaccct gcaaaccgac gaaggcgcaa ctttcgatac cgttgtcact
     4261 ctgcaagcag aagaaatttc accgcaggtc acctggggca ccaatccagg ccaggtgatt
     4321 tccgtgaacg acaatattcc cgatccggct tcgtttgccg atccggttga acgcgcgtcg
     4381 gcagaaaaag cgctggccta tatggggctg aaaccgggca ttccgctgac cgaagtggct
     4441 atcgacaaag tgtttatcgg ttcctgtacc aactcgcgca ttgaagattt acgcgcggca
     4501 gcggagatcg ccaaagggcg aaaagtcgcg ccaggcgtgc aggcactggt ggttcccggc
     4561 tctggccagg taaaagccca ggcggaagcg gaaggtctgg ataaaatctt tattgaagcc
     4621 ggttttgaat ggcgcttgcc tggctgctca atgtgtctgg cgatgaacaa cgaccgtctg
     4681 aatccgggcg aacgttgtgc atccaccagc aaccgtaact ttgaaggccg ccaggggcgc
     4741 ggcgggcgca cgcatctggt cagcccggca atggctgccg ctgctgctgt gaccggacat
     4801 ttcgccgaca ttcgcaacat taaataagga gcagaccatg gcagagaaat ttatcaaaca
     4861 cacaggcctg gtggttccgc tggatgccgc caatgtcgat accgatgcaa tcatcccgaa
     4921 acagtttttg cagaaagtga cccgtacggg ttttggcgcg catctgttta acgactggcg
     4981 ttttctcgat gaaaaaggcc aacagccaaa cccggacttc gtgctgaact tcccgcagta
     5041 tcagggcgct tccattttgc tggcacgaga aaacttcggc tgtggctcct cgcgtgagca
     5101 cgcgccctgg gcattgaccg actacggttt taaagtggtg attgcgccga gttttgctga
     5161 catcttctac ggcaatagct ttaacaacca gctgctgccg gtgaaattaa gcgatgcaga
     5221 agtggacgaa ctgtttgcgc tggtgaaagc caatccgggg atccatttcg acgtggatct
     5281 ggaagcgcaa gaggtgaaag cgggagagaa aacctatcgc tttaccatcg atgccttccg
     5341 ccgccactgc atgatgaacg gtctggacag tattgggctt accttgcagc acgacgacgc
     5401 cattgccgct tatgaagcaa aacaacctgc gtttatgaat taatcccctt gcccggtcaa
     5461 ataaccgggc tttccgctat cgtccacgtc atcaaacgtc tttaaccttt gcggttaaaa
     5521 ataatgcgac aacagaaata accgcaatta cccagtagac agcaaagtgc ccccaacttt
     5581 gtgcaattgc tccctgaata acgccagcca gaattacccc ggtagaaata ctgttagtaa
     5641 ataaggtggt agctgccccc gctcttccag gcattaaatc ctgaaaccat agcatcccaa
     5701 tacccgcaac aatgccgata aatacagcgt taaaaagttg cagcgtcatc aacgccatac
     5761 ggctatgaaa gaaaatcaat ccggtgtaaa acagtactcc tgccgccact gctatgacca
     5821 tcattcgccg cttaccataa cgtttgacat agtagccagc cagaatcatt gctggtattt
     5881 ccagtccagc tgccgtcccc atcaggaaac ccgccagttt gtctggcaat cctaactcgc
     5941 tactgatcca caacggcata tcaataatgt acatggtgtt gcaggtccac attaacgtcg
     6001 aggcgacaaa taacatccgt acgttactat cctgccagcc accttgcatt gataaagcat
     6061 tttccgacgg cagttctacc cgcgccacag acggaagcat aaatgcaatc aataccagac
     6121 tgagtgtgaa tatcccggcg gcaatcgaaa acatcaccac ttctcgcgcc gagttatccg
     6181 catattcccg cgccagagca aataactgtg gcattgccgt attggccaga gatgccagaa
     6241 gcacaccaca ggtgataagc gtcagataat gacgattaaa tgcaaacaat ggcgcattgc
     6301 cgatagccat caaacagcaa aatataatca gttttcgccg atcgccctga ctgtcagaac
     6361 gttttgccaa ccagaggctt acgccgatcc cagcaatagt attcaccgta taaaagagtc
     6421 cgatccagaa aggttgcgcg ccaacctcac gactcagaaa taagctcaat gtaggagcct
     6481 gtagcgcccc ggccaccccc atcataaaag cgaccagcat aaatgccgcg taaacaccgt
     6541 tcatacggcg agccatcgtc attatccaga tcatacgttt cctttttagc gcggcgagaa
     6601 taaaaaaaac cagcaggtat aatctgctgg cgggtgattt tacaccaata ctcagtcaca
     6661 catgatgcag gcaagtcaac tttcagaatt gcggtcatcc cactgcatca gtccttcaag
     6721 catggttaat cgttgtggga cgcttaacca acgcaaccag cacaacttcg ctgtcgcggt
     6781 aaaatagtgc tgataaaact gacgcatggg gcacctcctt gcttcatcgt taggaattat
     6841 tgacttaata tagggaaaat aaaattgctg tcttttgcgc aggagttccc cttttatgcc
     6901 atctgctcgt ctgcaacaac agttcatccg cctgtggcaa tgctgcgagg gtaaatcgca
     6961 ggacacaacg ctcaacgaac tggcagcgtt attgagctgc tcgcgtcgtc atatgcgtac
     7021 cctgctcaac accatgcagg atcgcggctg gctgacgtgg gaagcggaag tcgggcgcgg
     7081 taaacgctcg cgtctgacat tcctctatac cgggctggcg cttcagcaac agcgggcgga
     7141 agacctgctg gagcaggatc gtatcgacca actagtgcag ttggttggcg acaaagcgac
     7201 tgtgcggcaa atgctggttt ctcatctggg ccgcagcttc cgccaggggc ggcacatcct
     7261 gcgcgtgctc tactatcgtc cgttgcgtaa tctgctacct ggcagcgcat tgcgccgttc
     7321 cgaaacccat attgcccggc aaatcttcag ttcgctaacg cgcataaatg aggaaaatgg
     7381 ggaactggaa gcagacatcg cccaccactg gcagcaaata tcaccgcttc actggcgttt
     7441 ctttttgcgt ccaggagtcc attttcatca tggtcgtgaa ctggaaatgg acgacgtgat
     7501 cgcctcttta aaacgaatca atacgctgcc gctctattcg catattgcgg acattgtgtc
     7561 gccgacgccc tggacgctgg atatccatct cacgcaaccg gaccgctggt taccgttact
     7621 gctggggcaa gttccggcga tgatcctgcc gcgcgaatgg gaaaccctca gtaactttgc
     7681 cagccatccc atcggcaccg gtccgtatgc ggtgatccgc aacaccacca atcaactgaa
     7741 aattcaggca ttcgatgact tcttcggtta ccgggcatta atcgatgaag ttaacgtctg
     7801 ggttctgccg gaaattgccg acgagccagc tggaggtctg atgctaaaag gtccacaggg
     7861 tgaggaaaaa gagattgaaa gccgcctgga ggaaggttgc tactatttac tattcgacag
     7921 ccgcacccat cgcggagcga atcagcaagt cagggactgg gtaaactatg tgctttctcc
     7981 aactaatctg gtctatttcg ctgaggaaca gtaccagcaa ctgtggttcc cggcttatgg
     8041 actgctcccc cgttggcacc atgcccgcac cataaagagc gaaaaaccgg ctggcctgga
     8101 aagcctcacc ctgacctttt atcaggatca cagtgagcat cgggtgattg ccgggatcat
     8161 gcagcagatt ctggcaagtc accaggtcac gctggaaatc aaagagatca gctacgatca
     8221 gtggcatgaa ggagagatcg agagcgatat ctggcttaat agcgccaact ttacgctgcc
     8281 gctggatttt tcgctgttcg cgcacttgtg cgaggtgccg ctgctccaac actgtattcc
     8341 gatcgactgg caagccgacg ccgcccgctg gcgcaatggc gaaatgaacc tggcgaactg
     8401 gtgccagcaa ctggtcgcca gtaaagcaat ggtgccgctt atccaccact ggctgatcat
     8461 tcagggacaa cgcagtatgc gcggcctgcg tatgaatacc ctcggctggt tcgattttaa
     8521 atcagcgtgg tttgcgccgc cggatccatg attgctggta gataacaaaa tcactacact
     8581 aacgccgttc tcaacggggt gccacgcaaa cgcgtgcgct gagaaaatac ccgtcgaacc
     8641 tgatccggat aacgccggcg aagggatttg aggctccttc tcaagtcctt tgccactctt
     8701 ttttgaggtg caaagtgtta aaaaaatgtc ttcccctgct gttgctgtgc acagcgcccg
     8761 ttttcgctaa acccgttctg actgtttata cctacgattc cttcgccgcc gactgggggc
     8821 ctggtccaaa gatcaaaaaa gcttttgaag ccgactgtaa ctgcgaactg aaactggtgg
     8881 cgctggaaga tggcgtttcg cttctcaacc gtctacggat ggaaggcaaa aatagtaaag
     8941 ccgatgtggt gctggggctg gataacaatc tgttagacgc cgccagtaaa accggactat
     9001 ttgccaaaag cggtgtggca gcggatgccg ttaacgttcc cggcggctgg aataatgaca
     9061 ctttcgtccc gtttgattac ggctatttcg ccttcgtcta tgacaagaac aaactgaaaa
     9121 acccgccgca aagcctgaaa gaattggttg agagcgatca aaactggcgg gtgatttatc
     9181 aggatccgcg caccagtacg ccggggctgg gcctgctgtt gtggatgcaa aaagtctatg
     9241 gcgatgacgc cccacaagcc tggcaaaaac tggcgaagaa aaccgtcacg gtcaccaaag
     9301 gctggagcga agcctacggc ctgtttttaa aaggtgaaag cgatctggta ctgagttaca
     9361 ccacctctcc ggcttatcac attctcgaag agaaaaaaga taactacgcc gccgcgaact
     9421 ttagcgaagg tcactatctg caggtggaag tcgccgcccg caccgctgcc agcaagcagc
     9481 cggagctggc gcaaaaattc ctccagttta tggtttctcc ggcttttcag aatgcgatcc
     9541 caaccggtaa ctggatgtat ccggtggcaa atgtcacgct gcctgccggg tttgaacaat
     9601 tgaccaaacc ggccaccacg ctggagttca caccagctga agtggcggca caacgtcagg
     9661 catggattag cggatggcaa cgcgccgtca gccgttaatt cccggctggt taattccagg
     9721 tgtaagcgcc gccacgctgg tggtagcggt tgcgctcgcg gcgtttctcg ccctgtggtg
     9781 gaacgcgccg cagggtaact gggtggcagt ctggcaggac agctacctgt ggcatgtggt
     9841 gcgcttctcc ttctggcagg cgtttctctc ggcgctactc tctgtcgtac ccgcgatatt
     9901 cctcgcccgc gccctctatc gcaggcgctt tccgggtcgg ctggcgctgt tgcgtctgtg
     9961 cgcaatgacc ttgatcctcc cggtgctggt tgctgttttc ggcattctta gcgtctatgg
    10021 tcgccagggc tggctggcat cgctctgcca atcgctcggt ctggagtgga ccttttcgcc
    10081 ctacggcctg caaggtattt tgctggcgca cgtatttttt aatctgccga tggcgagccg
    10141 cttattactc caggcactgg aaaacattcc cgacgaacaa cgtcagcttg ccgcccagct
    10201 tgggatgcgc ggctggcatt ttttccgctt cgtcgaatgg ccgtggttac ggcgacaaat
    10261 cccgccggtt gctgcactta tctttatgct ctgtttcgcc agcttcgcca ccgtgctatc
    10321 actgggcggc ggtccgcagg cgaccactat cgagctggct atttatcagg cgctgagtta
    10381 cgactacgat cctgcccgcg cggcgatgct ggcgctgatc cagatggtgt gttgccttgg
    10441 gctggtactg ttgagtcagc gattgagtaa ggccattgcg ccaggcacca cgctgctgca
    10501 aggctggcgc gacccggacg atcgtctgca tagccgcatt tgcgacacgg tgttaatagt
    10561 gctggcgttg ctgctgttgt tgccaccgtt gctggcggtg atcgtcgatg gggtaaatcg
    10621 ccagttgccg gaagtgctgg cacaaccggt gctgtggcag gcgctgtgga cctcgttgcg
    10681 tattgcgctg gcggcaggtg tattgtgcgt agtgctgacc atgatgctgc tatggagcag
    10741 tcgcgaactt cgggcacggc agaaaatgct ggcgggtcag gcgctggaga tgagcggcat
    10801 gttgattctc gccatgccgg ggattgtgct ggctaccggc ttctttttac tgctcaacaa
    10861 cactatcggc ctgcaacaat ctgctgacgg cattgtgatt ttcaccaatg cgttaatggc
    10921 gatcccttat gcgctgaaag tactggaaaa cccgatgcgc gatatcaccg cccgctacag
    10981 tatgttgtgt cagtcgctgg gcattgaagg ctggtcgcgc ttaaaagtgg tggagctgcg
    11041 cgccctgaaa cgtccactgg cgcaggcgct ggcctttgcc tgcgtgctgt cgattggtga
    11101 ttttggcgtg gtggcgttgt tcggtaacga tgatttccgt accctgccgt tttatctcta
    11161 ccagcaaatt ggctcctatc gcagccagga cggcgcggtc accgcgttaa ttctgctgct
    11221 actctgtttt ctgctgttta ccgtgattga aaaactaccg gggcgaaatg ttaaaactga
    11281 ctgatatcac ctggctttac caacatttgc cgatgcgttt tagcttaacg gtggaacgcg
    11341 gcgagcaggt ggcgatcctc gggccaagcg gcgcaggtaa aagtaccctg ctgaatttga
    11401 ttgccggttt tctgacgcca gccagcggtt cgctgactat cgatggcgtt gatcacacca
    11461 cgacgccgcc gtcacgccgt ccggtgtcga tgctgtttca ggagaacaac ctgttcagcc
    11521 acctgacggt cgcgcagaac atcgggctgg ggcttaatcc aggattgaag ctgaacgcgg
    11581 cacagcagga gaaaatgcac gctatcgccc gtcagatggg gattgataat ttaatggcgc
    11641 ggttaccggg cgagctttcc ggcggccagc gacagcgcgt ggcgttagcg cgttgtctgg
    11701 tgcgcgaaca gccgatttta ttgctcgatg aaccgttctc tgcgctcgat ccggcgttac
    11761 gtcaggagat gctgacgctg gtgagcacga gctgccagca gcaaaaaatg acgctattga
    11821 tggtgtcgca cagtgtggaa gatgcggcgc ggatcgccac acgctcggtg gtggtcgccg
    11881 acgggcgcat cgcctggcag ggtaagactg aagaattgtt gagcggtaag gcgagtgctt
    11941 cggcaatatt ggggattacg ggttagtatg cactgtcaga tgcggcgtga acaccttatc
    12001 cgacctacgg acagtgcgtt ttgtaggcct gataagaccg gtcagcgtcg catcaggcaa
    12061 cccgcacaag acaatcccct aaaccccaac cactttacgc aaaatatcga tatacaccgg
    12121 catcaacggg tggcgaatta acaccaccag cgccaccacg ccgatggcag aaatcaacgg
    12181 cgtcagccac aacaaacggc cgcgcgacaa ataatgactt aaacgatcgg ttgctttacc
    12241 gctgcgccat aaccgccagc acagccagcc accaacccac aaaaataccg ccgtcgccag
    12301 cagcaaccat ttaaactcgc cgctctgcat tccggctgga atatcgatcg ccgcgcccgc
    12361 cagaatccca ggcaggaagt aaaacggtgg ccacagcagg cagccgataa tattcggcgt
    12421 aatgaatttt gccaccggca gatccagcat tcccgccacc attggcacca gcggacgcgt
    12481 cgggccaaca aagcgaccga ccagaatggt gaacatgctg tgttgatgca acgcatgttc
    12541 agttttatcg agtaatgctt tgtttttctt cagaaatgac cagcgatgca acggcttttt
    12601 aaaacgccag cccagccaga aggagatcca gtcgcccagc agacaaccga caatccctgc
    12661 cagccaggcg tgccagaaac ttaactcgcc gctgccaatc agcgctccca gccccgccat
    12721 cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc gactccagaa aggccaccaa
    12781 caccaccgcc atcaatgaat acacggtaga ttgggtaata aagtgttcca gcaatgcttg
    12841 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
    12901 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
    12961 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
    13021 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
    13081 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
    13141 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
    13201 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
    13261 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
    13321 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
    13381 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
    13441 ccgggcgaaa gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
    13501 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
    13561 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
    13621 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
    13681 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
    13741 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
    13801 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
    13861 gacattgccg tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc
    13921 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
    13981 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
    14041 attccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
    14101 tctctactgt ttctccatac ccgttttttt ggatggagtg aaacgatggc gattgcaatt
    14161 ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg tggactgcgc cagcggtgaa
    14221 gagatcgcca ccagcgtaga gtggtatccc cgttggcaaa aagggcaatt ttgtgatgcc
    14281 ccgaataacc agttccgtca tcatccgcgt gactacattg agtcaatgga agcggcactg
    14341 aaaaccgtgc ttgcagagct tagcgtcgaa cagcgcgcag ctgtggtcgg gattggcgtt
    14401 gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg gtaacgtgct ggcgctgcgc
    14461 ccggagtttg ccgaaaaccc aaacgcgatg ttcgtattgt ggaaagacca cactgcggtt
    14521 gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc
    14581 tacattggtg gtatttattc cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc
    14641 caggacagcg ccgtggcgca atctgccgca tcgtggattg agctgtgcga ctgggtgcca
    14701 gctctgcttt ccggtaccac ccgcccgcag gatattcgtc gcggacgttg cagcgccggg
    14761 cataaatctc tgtggcacga aagctggggc ggcttgccgc cagccagttt ctttgatgag
    14821 ctggacccga tcctcaatcg ccatttgcct tccccgctgt tcactgacac ctggactgcc
    14881 gatattccgg tgggcacctt atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc
    14941 gtggtgattt ccggcggcgc gtttgactgc catatgggcg cagttggcgc aggcgcacag
    15001 cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat tgccgacaaa
    15061 cagagcgttg gcgagcgggc agttaaaggt atttgcggtc aggttgatgg cagcgtggta
    15121 cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta cgcctggttt
    15181 ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatccgga actgaaagcg
    15241 caaatcaacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg ggccaaaaat
    15301 ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggccgccg cacaccgaac
    15361 gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga cgctccgctg
    15421 ctgtttggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt
    15481 accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc gcggaaaaac
    15541 caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct
    15601 gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca
    15661 gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc
    15721 agcgaacaga cacaacgctt tgaacagctt tatcgccgct atcagcaatg ggcgatgagc
    15781 gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc cgttccgact
    15841 ctataaggac acgataatga cgatttttga taattatgaa gtgtggtttg tcattggcag
    15901 ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg agcacgttgt
    15961 taatgcgctg aatacggaag cgaaactgcc ctgcaaactg gtgttgaaac cgctgggcac
    16021 cacgccggat gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg
    16081 tctggtggtg tggctgcaca ccttctcccc ggccaaaatg tggatcaacg gcctgaccat
    16141 gctcaacaaa ccgttgctgc aattccacac ccagtttaac gcggcgctgc cgtgggacag
    16201 tatcgatatg gactttatga acctgaacca gactgcacat ggcggtcgcg agttcggctt
    16261 cattggcgcg cgtatgcgtc agcaacatgc cgtggttacc ggtcactggc aggataaaca
    16321 agcccatgag cgtatcggct cctggatgcg tcaggcggtc tctaaacagg atacccgtca
    16381 tctgaaagtc tgccgttttg gcgataacat gcgtgaagta gcggtcaccg atggtgataa
    16441 agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg gcgatctggt
    16501 gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag
    16561 ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga acgtgctgga
    16621 agcggcgcgt attgagctgg ggatgaagcg tttcctggaa caaggtggct tccacgcgtt
    16681 caccaccacc tttgaagatt tgcacggtct gaaacagctt ccaggtctgg ccgtacagcg
    16741 tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct
    16801 tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta tggaggacta
    16861 cacctatcac ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc tggaagtctg
    16921 cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt cagcatctcg gtattggtgg
    16981 taaggacgat cctgcccgac tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag
    17041 cctgattgat ctcggcgatc gttaccgtct gctggttaac tgtatcgaca cggtgaaaac
    17101 accgcactcc ctgccgaaac tgccggtggc gaatgcgctg tggaaagcgc aaccggatct
    17161 gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg caccataccg tcttcagcca
    17221 tgcgctgaac ctcaacgata tgcgtcagtt cgccgagatg cacgacattg aaatcacggt
    17281 gattgataac gacacccgcc tgccagcgtt taaagacgca ctgcgctgga acgaagtgta
    17341 ttacggattt cgtcgctaag tagccacatc aggtatgtaa cgcctgatgc gacgctgacg
    17401 cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc tcatccggca
    17461 ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct acgcgctgcg attttgtaga
    17521 ccggataagc gaagcgcatc cggcacgaag gagtcaacat gttagaagat ctcaaacgcc
    17581 aggtattaga agccaacctg gcgctgccaa aacacaacct ggtcacgctc acatggggca
    17641 acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt
    17701 acagcgtcat gaccgctgac gatatggtcg tggttagcat cgaaaccggt gaagtggttg
    17761 aaggtacgaa aaagccctcc tccgacacgc caactcaccg gctgctctat caggcattcc
    17821 cctccattgg cggcattgtg catacgcact cgcgccacgc caccatctgg gcgcagtcgg
    17881 gtcagtcgat tccagcaacc ggcaccaccc acgccgacta tttctacggc accattccct
    17941 gcacccgcaa aatgaccgac gcagaaatca acggcgaata tgagtgggaa accggtaacg
    18001 tcatcgtaga aacctttgaa aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg
    18061 tccattccca cggcccgttt gcatggggca aaaatgccga agatgcggtg cataacgcca
    18121 tcgtgctgga agaggtcgct tatatggaga tattctgccg tcagttagcg ccgcagttac
    18181 cggatatgca gcaaacgctg ctggataaac actatctgcg taagcatggc gcgaaggcat
    18241 attacgggca gtaatgactg tataaaacca cagccaatca aacgaaacca ggctatactc
    18301 aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca ggttttatct taacccgaca
    18361 ctggcgggac accccgcaag ggacagaagt ctccttctgg ctggcgacgg acaacgggcc
    18421 gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt attcccgccg atcaggttcc
    18481 ccgcgctcag catattttgc agggtgaaca aggctttcgc ctgacaccgc tggcgttaaa
    18541 ggattttcac cgccagccgg tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa
    18601 ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc
    18661 agaacgctat ctgatggagc ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca
    18721 caatggcact atcgttaatg cccgtctgaa accgcatccc gactatcgtc cgccgctcaa
    18781 gtgggtttct atagatattg aaaccacccg ccacggtgag ctgtactgca tcggcctgga
    18841 aggctgcggg cagcgcatcg tttatatgct ggggtcggag aatggcgacg cctcctcgct
    18901 tgatttcgaa ctggaatacg tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg
    18961 gtttgccaac tacgatcctg atgtgatcat cggttggaac gtggtgcagt tcgatctgcg
    19021 aatgctgcaa aaacatgccg agcgttaccg tcttccgctg cgtcttgggc gcgataatag
    19081 cgagctggag tggcgcgagc acggctttaa aaacggcgtc ttttttgccc aggctaaagg
    19141 tcggctaatt atcgacggta tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt
    19201 ctcgctggaa actgtcgctc aggagctatt aggcgaagga aaatctatcg ataacccgtg
    19261 ggatcgaatg gacgaaattg accgccgttt cgccgaagat aaacctgcgc tggcaactta
    19321 taacctgaaa gattgcgagc tggtgacgca gatcttccac aaaactgaaa tcatgccatt
    19381 tttactcgaa cgggcaacgg tgaacggcct gccggtggac cgacacggcg gttcggtggc
    19441 ggcatttggt catctctatt ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct
    19501 cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg atggattcac ggccagggct
    19561 ttatgattca gtgctggtgc tggactataa aagcctgtac ccgtcgatca tccgcacctt
    19621 tctgattgat cccgtcgggc tggtggaagg catggcgcag cctgatccag agcacagtac
    19681 cgaaggtttt ctcgatgcct ggttctcgcg agaaaaacat tgcctgccgg agattgtgac
    19741 taacatctgg cacgggcgcg atgaagccaa acgccagggt aacaaaccgc tgtcgcaggc
    19801 gctgaaaatc atcatgaatg ccttttatgg cgtgctcggc accaccgcct gccgcttctt
    19861 cgatccgcgg ctggcatcgt cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa
    19921 agcgttgatt gaagcacagg gctacgacgt tatctacggc gataccgact caacgtttgt
    19981 ctggctgaaa ggcgcacatt cggaagaaga agcggcgaaa atcggtcgtg cactggtgca
    20041 gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa cggctgacca gcgcattaga
    20101 actggagtat gaaacccatt tctgccgttt tctgatgcca accattcgcg gagccgatac
    20161 cggcagtaaa aagcgctacg ccgggctgat tcaggagggc gataaacagc ggatggtgtt
    20221 taaagggctg gaaaccgtgc gcaccgactg gacgccgctg gcccagcagt ttcagcagga
    20281 gctatacctg cgcatcttcc gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga
    20341 caaactgatg gcgggtgaac tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc
    20401 gctgagcgag tatcagcgta atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga
    20461 agaaaaccaa aagcgtggtc gccccttgca atatcagaat cgcggcacca ttaagtacgt
    20521 atggaccacc aacggcccgg agccgctgga ctaccaacgt tcaccactgg attacgaaca
    20581 ctatctgacc cgccagctac aacccgtggc ggagggaata ctccctttta ttgaggataa
    20641 ttttgctaca cttatgaccg ggcaacttgg gctattttga gcaaaaaaag agttcgccag
    20701 ataccatttt gatgcgtgac gaatgctttg ccatccagta ccatagcgcc ctttccattc
    20761 ctggacctga ataacaccac tacctcataa gcacggtagc gggtggttat tgcctgcaat
    20821 taaagatata gagccgaaca catatgcctt ttacacttgg tcaacgctgg atcagcgata
    20881 cagaaagcga attgggactt ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac
    20941 ttttcccatc tactggtgaa aaccgtctgt acgcacgcag tgattccccc gtgacccgcg
    21001 tgatgttcaa ccctggtgat accattacca gccatgacgg ctggcagatg caagtcgaag
    21061 aagtaaaaga agaaaatggc ttgctgacct atatcggtac tcgcctggat actgaagagt
    21121 ccggcgtagc cctgcgtgaa gttttccttg atagcaaact ggtgttcagc aaaccgcagg
    21181 accgcctgtt tgccgggcag attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc
    21241 gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca
    21301 gcctgatccc gcaccagctc aacatcgctc atgatgttgg ccgccgccac gcgccgcgcg
    21361 tcctgctggc tgacgaagtg ggtttaggga aaaccattga agccgggatg atcctgcatc
    21421 agcaactgct ctctggcgct gctgaacgtg tgctgattat cgtcccggaa accttacagc
    21481 atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg tttgatgatg
    21541 agcgttatgc cgaagctcag cacgatgctt acaacccgtt cgacaccgaa cagctggtga
    21601 tttgctcgct ggattttgcc cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg
    21661 aatgggacct gctggtggtc gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa
    21721 gccgtgaata tcaggccatt gaacaactgg cagagcacat gccgggcgtt ctgctgctga
    21781 ccgcgacccc ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc
    21841 cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca gaaaaattat cgtccggttg
    21901 cggacgccgt tgccatgctg ctggcaggta acaaactgag caatgacgaa ctgaacatgc
    21961 tcggcgagat gatcggcgag caggatatcg agccgctgtt gcaggcagca aacagcgaca
    22021 gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat gctgatggat cgccacggca
    22081 ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa aggattcccg aaacgcgagc
    22141 tgcacaccat taagctgccg ctaccgacgc agtatcagac ggctattaaa gtctccggca
    22201 ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga tatgctctac ccggagcgta
    22261 tttatcagga atttgaaggt gataacgcca cctggtggaa cttcgatccg cgcgttgagt
    22321 ggctgatggg ctacctgacc agccatcgct ctcagaaagt gctggtgatc tgcgccaaag
    22381 ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg
    22441 tgttccacga aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag
    22501 aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc
    22561 agttcgccag ccacatggtg atgtttgacc tgccattcaa cccggatcta ctggagcagc
    22621 gtattggtcg tctggatcgt atcggccagg cgcacgatat tcagatccat gtgccttatc
    22681 tggagaaaac cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg gatgcatttg
    22741 agcacacctg cccgaccgga cgcactattt acgatagcgt atacaacgat ctgattaact
    22801 atctggcttc accggatcaa accgaaggct ttgacgatct gatcaaaaac tgccgcgagc
    22861 aacatgaagc gctgaaagca cagctggaac agggtcgtga ccgcctgctg gaaatccact
    22921 ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat tgaagagcag gatgacgata
    22981 ccaacctgat cgccttcgcc atgaacctgc tcgatattat cggtatcaat caggacgatc
    23041 gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat gctggtgccg gacttccctg
    23101 gcctgtcgga agatggcatc accatcacct ttgatcgtga agtggcgctg gcgcgtgaag
    23161 atgcacagtt tattacctgg gagcatccgc tgatccgcaa cggtctggat ctgatccttt
    23221 ctggcgatac cggtagcagc acgatttcac tgttaaaaaa caaagcgttg ccggtaggta
    23281 cgctgttggt ggaactgatt tatgtggttg aagcccaggc tccgaagcag ttgcagctca
    23341 accgcttcct gccaccgacg ccggtacgta tgctgctgga taaaaacggc aacaacctgg
    23401 cggcgcaggt agagtttgaa acctttaacc gccagcttaa cgcggttaac cgtcacaccg
    23461 gcagcaaact ggttaacgcc gtgcagcagg atgttcacgc tatccttcaa ctgggtgaag
    23521 cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa gccgacgaaa
    23581 aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg aacattcgtg
    23641 acgacgaact gaccgccatt gagagcaacc gtcagcaggt aatggaaagc ctggatcagg
    23701 caggttggcg tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa
    23761 aatggggatg gaaaactaca atccaccgca ggaaccctgg ttggttatcc tgtatcagga
    23821 tgaccacatt atggtggtta acaagccgag cggtttgttg tctgtgccgg ggcgtctgga
    23881 agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt
    23941 gcatcgtctg gatatggcta ccagcggcgt gattgtagtg gcgctgacca aagccgcgga
    24001 gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg tggcccgcgt
    24061 ctgggggcat ccatcccctg cggaaggtct ggtggatctg ccgctgattt gcgactggcc
    24121 aaaccgcccg aaacagaaag tctgttacga aacgggtaaa cctgcgcaga cggaatatga
    24181 agtggtggag tatgcggcgg ataacacggc aagagtggtg ttaaaaccga ttactgggcg
    24241 ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat ccgattctcg gcgatcgttt
    24301 ttatgcatca ccagaagcga gagcgatggc accgcgtttg ttgctacatg cagagatgtt
    24361 gacgattacc catccggcgt atggcaatag tatgacgttt aaagcgccag cggattttta
    24421 atagtcacgc tggcgcagtt cggtaggcgg ataaggcgtt cacgccgaat ccggcaattg
    24481 tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca ggcctacagt ttaaaactta
    24541 tttaaaccct ttctgctgct ttatcagctc atatgcctgc tgaatttcct gcgctttctg
    24601 cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat cgggatggtg
    24661 ttcactcatc agcttacggt aggctcgttt aatggtggtc gcatcatccg tcggcttcac
    24721 acccagcaca ttacaggcat cttccagcgt cgggccacgc tgcgcttgct gccagttacc
    24781 accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa
    24841 aaactggtca aactgggcgc gggagatccc taattcttct gcaatgacat acagcaccgc
    24901 ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga tctccagaaa
    24961 catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca tcttttcgcg
    25021 cagcgggtaa ttgtctgatt ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc
    25081 gccatgaagg ttcattcggt ccatcagctg gctggcgata tgaatatcag cctccgtgac
    25141 gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc
    25201 ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt tatcaaacat
    25261 atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca gtaaggccac
    25321 ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac acacggatat
    25381 cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgca gtcaattgtg
    25441 ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc taacaccact
    25501 gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta gtctctgacc
    25561 gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta
    25621 tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag ggactggcag
    25681 ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct ctggtacagg
    25741 gcgataccaa tgacttaccc gtgactatca atgctgacca cgcgaaaggg gactacccgg
    25801 atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt ctgcaggccg
    25861 acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta cgaaccgttg
    25921 atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg ccgaaaggct
    25981 gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag atggtgggtc
    26041 gccagggccg cggtaaagcg gacctgatga aacaacgtgg tgaaaaccgc tataccattc
    26101 tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc gtggtaggta
    26161 gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc cgctttaagg
    26221 tgggtccagt accgatcttt tatagcccct atttgcagtt gccggtgggt gacaaacgtc
    26281 gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt gagttctacc
    26341 tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg cattatatgc
    26401 atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag gcgggcgctg
    26461 gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa cacccgaacg
    26521 atgacagttc acgtcgttgg ttgttctact ggcaacactc cggggtcatg gatcaagtgt
    26581 ggcgtttcaa cgtagattac accaaggtca gcgatcctag ctacttcaat gatttcgata
    26641 acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt ggctatgcgg
    26701 tgcaaaactt caatgccacc gtttcaacca agcagttcca ggtctttagc gagcagaaca
    26761 ccagtagcta ctcggcagag ccgcagttag acgttaacta ctaccagaat gatgttggtc
    26821 cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga gacgacatgc
    26881 ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct aataactggg
    26941 gcagcatcaa taccgaagcg aagttgctgg caacccatta tcagcaaacc aatcttgact
    27001 ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgtt atgccgcaat
    27061 tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct ccgggttata
    27121 cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca
    27181 tctataacta cgactcgtct ctactgcaat ctgactactc tggcctgttc cgggaccgga
    27241 cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt gtcacatctc
    27301 gcatatatga tgatgctgcc gttgaacatt ttaatatttc cgttggtcaa atctactatt
    27361 tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac aaaacgggct
    27421 cactggtgtg ggcaggcgat acttactggc gtatctccga gcgttgggga ttgcgtggcg
    27481 ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc attgaatacc
    27541 gtcgggatga agaccgtctg gtacagctga attaccgtta cgccagcccg gaatatattc
    27601 aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt atttcgcagg
    27661 taggtgctgt cgccagctgg ccaattgccg atcgttggtc cattgttggg gcctactact
    27721 acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac agctcctgct
    27781 gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac gataaacaac
    27841 atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg agctccaact
    27901 acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa aacactttgt
    27961 gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa aagtatgaag
    28021 aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt cgctgccccc
    28081 caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt
    28141 gatggattaa tgcagtcggt aaaactgaac gctgcgcagg caaggcagca gcttcctgat
    28201 gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat catcctgcag
    28261 atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc gattgctaac
    28321 atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc ttacgatggt
    28381 ctgaactaca acacctatcg taaccagatc cgcaaagaga tgattatctc tgaagtgcgt
    28441 aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg aagtcgaatc cctggcgcag
    28501 caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat cctgatcccg
    28561 ctgccggaaa acccgacttc tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt
    28621 gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca ttctgccgac
    28681 cagcaggcgc tgaacggcgg ccagatgggc tggggccgta ttcaggagtt gccgggtatc
    28741 ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc
    28801 gttggcttcc atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg
    28861 accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac tgacgaacag
    28921 gcccgtgtga aactggaaca gattgctgcc gatatcaaga gtggtaaaac gacttttgct
    28981 gccgcagcga aagagttctc tcaggatcca ggctctgcta accagggcgg tgatctcggc
    29041 tgggctacac cagatatttt cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa
    29101 ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga actgctggat
    29161 acccgtaatg tcgataaaac cgacgctgcg cagaaggatc gtgcataccg catgctgatg
    29221 aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgcgc cagcgcctac
    29281 gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact cccggcgagc
    29341 ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac
    29401 tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc ggtttgccgc
    29461 tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactgcg ggcacattaa
    29521 cgctacttcc tgtcgcgcta cgcgaatctg tcactgcggg gcagttagcg attgaaaatg
    29581 ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg
    29641 ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaggt attccgttta
    29701 ctggtcatac cgagtttttc gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg
    29761 cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc gatatcgcag
    29821 atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac gatttgcgta
    29881 caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg
    29941 aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc gacgagctgc
    30001 gggcgcaggg gatgatactc aacgggccgc tgcctgccga taccctgttt cagccgaaat
    30061 atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa
    30121 aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt attcgcacat
    30181 cagtggacca cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt
    30241 ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga ataatcgagt
    30301 ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt
    30361 gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg
    30421 ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc tgacggtcat
    30481 cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag gcccgaaact
    30541 gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg agaaaatggg
    30601 tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt tgatgttcca
    30661 tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa aagaggtggt
    30721 gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg tcatggcgca
    30781 atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta caccaccacc
    30841 caaagtggat tccgccgtcg tgcgcctggt tcctcatgca acgatgcctc acccggttaa
    30901 agatgttcgt gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat
    30961 tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc
    31021 ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga actatctggc
    31081 ggagaacgcg cctttgcagg agagttaacg atgatcaatt cgccccgagt gtgtattcag
    31141 gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta cgtttttgct
    31201 tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg gcgttactgg
    31261 ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc
    31321 cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat cattgaaacc
    31381 ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt ccctttcagc
    31441 atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa gaatatggcg
    31501 acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt gctgcataaa
    31561 gtagaattta cccctgggaa agataccctc tggctgacgg gcgatctggt cgcgcgcggc
    31621 ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt acgtctggtg
    31681 ctgggcaatc acgatctgca tctgctggcg gtatttgccg ggatcagccg caataaaccg
    31741 aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct taactggctg
    31801 cgtcgccagc ctttgctgca aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg
    31861 atcacgccgc agtgggatct acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg
    31921 atatcgagtg actcctatcc cttctttctc gacgctatgt acggcgatat gccaaataac
    31981 tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt
    32041 atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc gccggaagag
    32101 gcccctgccc cactgaaacc gtggtttgcg attcctggcc ctgtcgctga agaatacagc
    32161 atcgcctttg gtcactgggc atcgctggag ggcaaaggta cgccggaagg tatatacgcg
    32221 ctggataccg gctgctgctg gggtggtaca ttaacctgcc tgcgctggga agataaacag
    32281 tattttgtcc agccgtcgaa ccggcataag gatttgggcg aagcggcggc gtcttaaaca
    32341 cagcctgata taggaaggcc ggataagacg cgaccggcgt cgcatccggc actagccgta
    32401 aattctatac aaaattaccg ccgctccaga atctcaaagc aatagctgtg agagttctgc
    32461 gcatcagcat cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccggg
    32521 aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt
    32581 ggcaggaact gctcataaac gcgaccgccg ccaatcacca tgatttctgg tacgtcacca
    32641 cacgccgcga tggcttcatc caccgacttc acccacgtta cgcgatcgtc cgtacccggt
    32701 tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga ttcccaggta
    32761 tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca
    32821 ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc taacgccgca
    32881 atcagactga tcattgagat ttcccgataa aaaaaattgt cgccactata cgtaaagcgt
    32941 aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa ttttatattc tgctggcgag
    33001 tccacgctct ctccctggac tcgccgcatt acaatgaaac aaaaacaaac agttagctgt
    33061 aaagtgtgat ttatgtcact ctttattagg atgagggttt cgtttccggt tcatccgcca
    33121 tgttgccggt atgtttacct tcttcggttc cctgccagcc atgtcgttga attaatgaca
    33181 gatgttcgcg gtcctcggta atgatctcac ttaacatcgc gctggtgcgt ttatagaccg
    33241 ccgcgcgggc tttggtgtcg ttctcaacca ttgccatctc ttccaccatc tgaatattaa
    33301 agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct aaactttcca
    33361 gtgccagacg cccggttttc agcgcacctt cgaaggtttc acgctccggc ttttcaacgc
    33421 ctgcctgacg caaacgaatg tagtggtcga catcgcgggc gcgggcaata atctgcaaat
    33481 gcgggaagtg ttctttcacc atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg
    33541 cgttaatcag cacttccgct ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg
    33601 catcgccata aaacactttc ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt
    33661 gatcgagcac caccattttc accccgctgg agagcagtaa acgtccggtg atctgcccaa
    33721 aacgaccgaa tccggcaatg attacccgcg gctgttcttc gtcgatctca tcggcttcac
    33781 gcgcctcttc agtagaagat tgctcaaggc gattgaggat caccagcaga atcggcgttg
    33841 ccgccatcga cagcgccacc gccagggtca gcgatttcgc ccactccggc tccagcacat
    33901 tcgccatctg cgccgcgcca aataccacaa aggcaaactc actgccctgc cctaacaaca
    33961 ccgcaaacca acgacgctgt ttatttggca cttgcaacgg tcgggcaatc agccacagca
    34021 tggcgatttt gatgatgagg aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa
    34081 gcagcgtgcc aaagtctatc gacatgccaa caccgatgaa aaacagcccc aacagcaaac
    34141 ctttaaaggg ttcgatatcg ctctccagcg catgacggta ttccgagctt gccagcagta
    34201 cgcccgccag aaacgcgccc atcgccatcg acaagccgac ctcttccagc agcaaaccaa
    34261 agccaaacac gaggaataac gccacggcac taaacacttc ccgcaagcca gagcgggcta
    34321 caaaacgcag cgccggacgc gtgacatagc gccccagcaa taccaccagc accagcgcac
    34381 ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg
    34441 ccagtagcgg aatcatcgcc accagcggga tcgccgcgat atcctggaac agcagcaccg
    34501 caaaggcact gcgacccatt tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg
    34561 caatcgccgt agaggagagc gccagcgtca tgccgatcaa ttccgcgacc tgccagcgca
    34621 gcccaagtaa catgcagaac agccccagca ggccgccgca aatcaccatc tgcaatgcgc
    34681 caccgccgaa cactgccgca cgcagcttcc acagcctttg tggatcgagt tcgaggccga
    34741 taataaacag catcagcacc accccaatct cggcaaagtg cagaatagat tcggcatcgg
    34801 tcaccagtcg cagcccccac gggccaataa tgcagccggc gatcaggtag ccaagtaccg
    34861 atcccagacc aagacgtacc gcaatgggta caatcagcgc tgccgaaccg agataaatca
    34921 gcgcctgaat cagcgtatgg ctatccatga tgggcctcct gccattccag cagacgttgc
    34981 ttatagtgac gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg
    35041 gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag cggctgcgac
    35101 agcacatcaa agcccggatg cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc
    35161 accgcccaca gcaaatgttt gccatgcagc gccttgccgc cgtgaccgta agcccagccg
    35221 tgcgacaaaa ctttatcgat ccaaagttta agaagcggag gaatgctgta ccactgcatc
    35281 ggatgctgcc agacgatcag atcggcgcga gacagcgcct cctgctcggc ggcaatatcg
    35341 atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc cagcgtcctt
    35401 gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg gatacggatg cgcataaatt
    35461 ataagaatca taggtagccc gcgtgattac gcctttgttt tcccacaaag tgtagtcata
    35521 tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc tgaacaattt
    35581 accccattct ctgcccgcgg atcgtcatat tgctggtttg cgccaatgac atccctcgat
    35641 gtctccgggg caaacgctac ggaaatcaac aagccaaaca gcgagatacc cgcccccatt
    35701 agcatcgtgt tactgatacc gtaattattg ataaagatcg gcagtgccca ggtcgaaaca
    35761 atggtgccaa tacggcttaa ggacataatc acgcccacgg cagaggcgcg gatatctgtc
    35821 gggaagagtt cattaggata gagccactgc aaattacccg gcccgccaga gaaaaaggca
    35881 tacaccgcaa aggccatcac taccagccag atccccatat ccgggatcag ccccaaaacc
    35941 gccagcgcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg tccggcagtg
    36001 tttaaccaca gcatcggcgg aatacagccg agcataaaga acaggctaat caccacgttc
    36061 cccagcgccg cgtttttgcc aacacccaat cccaacaaac caacgatttg cgggccaaag
    36121 gtgtaaatgg cgaacattgg gatcacctgg caggtccaga tggcggcaac aaacagaaca
    36181 aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg ctgctcttca
    36241 tcgaaagcca ccggttcgcc aaacagtttt atcatcatct cttcgcactc ttttactcgc
    36301 cctttgcgta ataaccagcg gggagattca ggcagttcga atcgaccaat caaaatcaac
    36361 aaacagggga tcgccgcgct acccagcatc cagcgccagc cgccttccac atcataaagc
    36421 cagtagccga ccagatcggc gcaggtcgcg ccgacatacc acatggcggc gataaagctg
    36481 atggaaaatg cccgctgacg ggtactggag aactcggtga tcattgaggt ggcgatggga
    36541 taatcggcac cgatgacaat gccgataagt acccgcatca ccaacagttc gacgggggat
    36601 gaaacaaaca tcgtcgccac cgatatcacg ccgatggcga tgatatcaat gaggaacatt
    36661 ttgcgccgtc cgactttatc ggaaatatag ccaaacagcg atgtgccaac gaacagcccg
    36721 gcgagcgttc ccgcgcccaa caagccaatc cagtcagcgt ccagttttag cgccggagtc
    36781 agttgctcca gcgccacgcc aatcattacc agtacataac catc
//
LOCUS       DADLTD010000014        36418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLTD010000014 DADLTD010000000
VERSION     DADLTD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.014"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            24..896
                     /locus_tag="JN701_000550"
     CDS             24..896
                     /locus_tag="JN701_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7363092.1"
                     /translation="MRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEEQIGYTLFT
                     RKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNTYPTIIFDQ
                     LISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENLTISELPP
                     LRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINPTIIYRPY
                     SFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRNHRYTAT
                     MLQHIAEDRDGTNH"
     gene            complement(893..2371)
                     /gene="scpC"
                     /locus_tag="JN701_000551"
     CDS             complement(893..2371)
                     /gene="scpC"
                     /locus_tag="JN701_000551"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY7363093.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETDKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2395..3180)
                     /gene="scpB"
                     /locus_tag="JN701_000552"
     CDS             complement(2395..3180)
                     /gene="scpB"
                     /locus_tag="JN701_000552"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY7363094.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3191..4186)
                     /gene="meaB"
                     /locus_tag="JN701_000553"
     CDS             complement(3191..4186)
                     /gene="meaB"
                     /locus_tag="JN701_000553"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY7363095.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4179..6323)
                     /gene="scpA"
                     /locus_tag="JN701_000554"
                     /pseudo
     CDS             complement(4179..6323)
                     /gene="scpA"
                     /locus_tag="JN701_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6527..7420)
                     /locus_tag="JN701_000555"
     CDS             complement(6527..7420)
                     /locus_tag="JN701_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY7363096.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7803..8462
                     /gene="rpiA"
                     /locus_tag="JN701_000556"
     CDS             7803..8462
                     /gene="rpiA"
                     /locus_tag="JN701_000556"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY7363097.1"
                     /translation="MMQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8717..9949
                     /locus_tag="JN701_000557"
     CDS             8717..9949
                     /locus_tag="JN701_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY7363098.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10178..10237
                     /gene="ibsC"
                     /locus_tag="JN701_000558"
     CDS             10178..10237
                     /gene="ibsC"
                     /locus_tag="JN701_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY7363099.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10570..10629
                     /locus_tag="JN701_000559"
     CDS             10570..10629
                     /locus_tag="JN701_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7363100.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10730..11332)
                     /locus_tag="JN701_000560"
     CDS             complement(10730..11332)
                     /locus_tag="JN701_000560"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY7363101.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11353..11536)
                     /gene="ssrS"
                     /locus_tag="JN701_000561"
     ncRNA           complement(11353..11536)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JN701_000561"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11578..11907)
                     /gene="zapA"
                     /locus_tag="JN701_000562"
     CDS             complement(11578..11907)
                     /gene="zapA"
                     /locus_tag="JN701_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY7363102.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12075..12653
                     /gene="ygfB"
                     /locus_tag="JN701_000563"
     CDS             12075..12653
                     /gene="ygfB"
                     /locus_tag="JN701_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY7363103.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12679..14004
                     /gene="pepP"
                     /locus_tag="JN701_000564"
     CDS             12679..14004
                     /gene="pepP"
                     /locus_tag="JN701_000564"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY7363104.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14001..15179
                     /gene="ubiH"
                     /locus_tag="JN701_000565"
                     /gene_synonym="visB"
     CDS             14001..15179
                     /gene="ubiH"
                     /locus_tag="JN701_000565"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY7363105.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15202..16404
                     /gene="ubiI"
                     /locus_tag="JN701_000566"
                     /gene_synonym="visC"
     CDS             15202..16404
                     /gene="ubiI"
                     /locus_tag="JN701_000566"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY7363106.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16478..16561)
                     /locus_tag="JN701_000567"
     CDS             complement(16478..16561)
                     /locus_tag="JN701_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363107.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16851..17945
                     /gene="gcvT"
                     /locus_tag="JN701_000568"
     CDS             16851..17945
                     /gene="gcvT"
                     /locus_tag="JN701_000568"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY7363108.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17969..18358
                     /gene="gcvH"
                     /locus_tag="JN701_000569"
     CDS             17969..18358
                     /gene="gcvH"
                     /locus_tag="JN701_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY7363109.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18477..21350
                     /gene="gcvP"
                     /locus_tag="JN701_000570"
     CDS             18477..21350
                     /gene="gcvP"
                     /locus_tag="JN701_000570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY7363110.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21616..22359
                     /locus_tag="JN701_000571"
                     /pseudo
     CDS             21616..22359
                     /locus_tag="JN701_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22416..23849)
                     /locus_tag="JN701_000572"
     CDS             complement(22416..23849)
                     /locus_tag="JN701_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7363111.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23894..24205
                     /locus_tag="JN701_000573"
     CDS             23894..24205
                     /locus_tag="JN701_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY7363112.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24369..25028
                     /locus_tag="JN701_000574"
     CDS             24369..25028
                     /locus_tag="JN701_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY7363113.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25224..26204)
                     /gene="ygfZ"
                     /locus_tag="JN701_000575"
     CDS             complement(25224..26204)
                     /gene="ygfZ"
                     /locus_tag="JN701_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY7363114.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26447..26713
                     /gene="sdhE"
                     /locus_tag="JN701_000576"
     CDS             26447..26713
                     /gene="sdhE"
                     /locus_tag="JN701_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY7363115.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26694..27101
                     /locus_tag="JN701_000577"
     CDS             26694..27101
                     /locus_tag="JN701_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HAY7363116.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27141..27662)
                     /gene="fldB"
                     /locus_tag="JN701_000578"
     CDS             complement(27141..27662)
                     /gene="fldB"
                     /locus_tag="JN701_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY7363117.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDILGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27774..28670
                     /gene="xerD"
                     /locus_tag="JN701_000579"
     CDS             27774..28670
                     /gene="xerD"
                     /locus_tag="JN701_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY7363118.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28695..29405
                     /gene="dsbC"
                     /locus_tag="JN701_000580"
     CDS             28695..29405
                     /gene="dsbC"
                     /locus_tag="JN701_000580"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY7363119.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29411..31144
                     /gene="recJ"
                     /locus_tag="JN701_000581"
     CDS             29411..31144
                     /gene="recJ"
                     /locus_tag="JN701_000581"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY7363120.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARALANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31235..32333
                     /gene="prfB"
                     /locus_tag="JN701_000582"
     CDS             join(31235..31309,31311..32333)
                     /gene="prfB"
                     /locus_tag="JN701_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY7363121.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32343..33860
                     /gene="lysS"
                     /locus_tag="JN701_000583"
     CDS             32343..33860
                     /gene="lysS"
                     /locus_tag="JN701_000583"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY7363122.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33903..34451)
                     /gene="idi"
                     /locus_tag="JN701_000584"
     CDS             complement(33903..34451)
                     /gene="idi"
                     /locus_tag="JN701_000584"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY7363123.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34506..34577
                     /locus_tag="JN701_000585"
     CDS             34506..34577
                     /locus_tag="JN701_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363124.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34574..34699)
                     /locus_tag="JN701_000586"
     CDS             complement(34574..34699)
                     /locus_tag="JN701_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363125.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34701..36150)
                     /locus_tag="JN701_000587"
                     /pseudo
     CDS             complement(34701..36150)
                     /locus_tag="JN701_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
BASE COUNT         8757 a         9182 c         9749 g         8730 t
ORIGIN      
        1 tggacatttt tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata
       61 ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg
      121 cgctggaaga acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca
      181 ataaagccgg tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga
      241 ttgataatga aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata
      301 atacttatcc gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg
      361 tgaccgccca gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc
      421 agttagattt tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc
      481 tgactatcag tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag
      541 agcgtcaaga gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc
      601 gggctaactt cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca
      661 ttatttatcg tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc
      721 tgacggtgat cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg
      781 atgcgccgca caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt
      841 ataccgccac gatgcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
      901 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
      961 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
     1021 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
     1081 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
     1141 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
     1201 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
     1261 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
     1321 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
     1381 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
     1441 ttgtcaggct ggaggcgctg gccccgctga ttttgtcggt ttccagtaaa tgcaccaccg
     1501 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
     1561 gcgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
     1621 gaatacgccc atgcgccatt tcctgcaata agaacgtgac cacgttatcg gcaatctgct
     1681 ggcacatggg attttgctta tccagcatat taccggcgtc gggcaagttg gtttccacga
     1741 cggcgacaat ctttttcgga tcgatttgca catagcgggt accgacgcga tccattgcat
     1801 ggaagatcga cacgctattg cgccgcggtg gcgcgccagg aatcacaata tctgccagtt
     1861 ctgcaacgcg cggatcgtga tagtgattaa gttcaatgat cactttcttc gctcgcagca
     1921 gccaggtcgg cgcattaccg atcccgctgg ttaaccagac tcgaccatcc ggtgccagtg
     1981 ccgatgcttc aatgacggca acatcaatgt cgccgaagaa accgtaattg accatttgcg
     2041 ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc ctgattgatc tttttacgta
     2101 aaccggacga tgtttgatat ggcgcacgcc aggaaacagc atcggcgtca gaaagtacat
     2161 cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg aatttgatac ggctttttgg
     2221 cctcatgctg ttcgttagct ctgcgggcaa tcgcggtggg tagggctttc ggcgaacccg
     2281 ccggggtaaa gccgctaaat gccaccatgt cgttatgctg gataatttct gccgcttcat
     2341 cggcggtcat ccttgtccac tgagtttcca tttttactcc ttcgttcgca gggattaatg
     2401 accaacgaaa ttaggtttac gtttttcgag gaaagcgttc attccttcct ggtaatcttc
     2461 gctgtcataa accgcgcgtc gcatcccctg aatacgttca aattcatcgg agttcatggt
     2521 gtgcgcttcg cccagtacac gcagctcttc tttgataaca gcaatggcta acggcgcttt
     2581 ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc agttcttcca cttccacaac
     2641 atggttgagg atgccgacag ccagcgcgcg ctgggcggtg attggcgaag cggtaaaaat
     2701 cagctctttg acaatgtgga agcccgcgtc gcgggtcagg ttgtgaatgc cgaccaggtt
     2761 atacgggacg ccgaggttta caggcgtcat tgagaaggtt gaggtactgg cggcgatgat
     2821 cagatcggaa ctcatgatca tttcaaatgc gccaccccaa acactacctt ccaccatcga
     2881 aatgatcggt ttcgggaatt tttggatcat gcgggtgatt tgacgcaatg gatcatcata
     2941 ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata tcgtgacctg cggagaagac
     3001 tttggatcca ctcggcgcgc gcaaaatgat acagcgaatt tccggccggt tgagatcgct
     3061 taacgcctgc ataagatcat caataaagac tttacttaag gcattaagtt ttcggccata
     3121 gttaaactca atgaccgcca ctttgttgat agtggcaacg ttcacatact gataagacat
     3181 aaaaattcct ttaatcaaaa tattgcgtct ggataaattc actgagctgc cgcaggccgg
     3241 tgcgcggtga gagcgtattg tttttgaccg ctaaaagcgt ctggcggtaa tagcgatcga
     3301 aatcttcatt cgcgaacagg tgattcagta cttcttcttc ggtctgctta cgcagccatt
     3361 ccaccgattg ttgttgccgc acttgttgta aacgaccact ggcagttagc gcggttttga
     3421 actcgatgat ggcgtgccag atctcatcga ttccacgttt ttccagtgcg ctacaagtca
     3481 gaacccgtgg ctgccattcg tcgtatttac gtcgcagaat atgcagggca ctctcgtaca
     3541 tatgccgggc aatggcgaca ttggtatggt tatcgccatc gtctttgttg ataacgatca
     3601 gatcagccac ttccatcagc ccttttttaa tgccctgcag atcatcgccg ccaccggcaa
     3661 tttgcaacga gataaaacag tccaccatgc gggcgacttc tgtttccgac tgcccgacgc
     3721 caaccgtttc gacaatcact acgtcataac ccgccgcttc gcataacagc attaattccc
     3781 gcgctcgctg actggcaccg cccagatgac cggaggatgg taccgggcga ataaacgccg
     3841 cttcggcacg cgccaggtca ttcatgcgga ttttatcccc gagaatgcta ccgccagtga
     3901 ccgggctgct gggatcgacc gcaataaccg cgacctttaa tccctctcga atcaacaaca
     3961 tgccaaaggc ctcaagaaag gtacttttcc ccgcgctggg ggtgccggta acgcccagtc
     4021 gcagggtgtt accgcagtac ggcataatgg catcaagcag ctgcgtactt agtgcctgat
     4081 gacgcgggtg acggctttcc accagcgtca tggcctgggc gagtgtggca cgctcaccct
     4141 gacgtaagcg gcgaatactt tctgccagcg tggcttcatt aatcatgatg ctggcttatc
     4201 agattcagta cgtcgcgcac actgtcgagc ataggtgtac ctggaccata aatcgccgcc
     4261 acgccgcgct cttgcaggaa ggcgtaatcc tgcggtggaa tgacgccacc ggcgactacg
     4321 cagatatctt cgcgtcccca ttttttcagc gcctcgacca gttccgggat cagcgtttta
     4381 tgaccggcag ccagtgagga tgcgcccact acgtgaacgt cattttctac tgccaggcgg
     4441 gcgatctctt caggtgtaga gaacatcggg cttaaatcta cgtcgaaacc gagatcggaa
     4501 taggcgctgg cgatcacttt cgagccgcga tcgtgcccat cctggcccat tttggcgatc
     4561 agaatgcgca ggcgacgacc attgtcggca aggaactgct ccgtttgcgc aacaatggca
     4621 tcgaactcgg aggccgattt ctcagattga tgatagcttt gcgcaatcac gccggtaaca
     4681 cactggcttg gcaccagata acggtcgaaa gcggcttcca gcgcatcgga aatttcaccc
     4741 agggtggcgc gaacgcgagc ggcattaaca gcggcagcca gcaggttttc gttatgctgt
     4801 gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg catcatcacg ggtggcgcga
     4861 atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca cgttgtcgat ctcaagtaca
     4921 tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac caacgatgac acgtttgccc
     4981 tggtcgataa gcgactgttc gcgcgctgag gcctattcga tcattcgttt tggcagacct
     5041 gcttcgatcg ctttcgccat accaccggct tcgtcgatct gttggataat agctctggct
     5101 tgtttgacga tttgatcggt cagcgactca atgtaatagg atccggccag tggatcgacg
     5161 gtgcggcaga gttctgattc ttcctggatg atgatctggg tgttgcgggc aatgcgtgct
     5221 gagaaatcgg taggcaaacc aagcgcttcg tcaaaggcgt tggtatgcag tgactgagta
     5281 ccgcccagcg tcgcagccag tgcttcaatg gtggtgcgga taacgttgtt atacggatcc
     5341 tgttcagtca ggctccagcc tgaggtctgg cagtgggtac gcagcgccag tgatttcggg
     5401 tcctgtgcgc caaatccact gaccgcttcg ctccataaat aacgtgccgc acgcaacatg
     5461 gcgacgttca taaacagatc catgccgatg ccgaagaaga acgacaggcg aggagcgaag
     5521 tcatcaattt tcagtccggc agagattgct gctttgatgt actcaatccc atcagcgagc
     5581 gtaaatgcta cctgctgcac gcagttggca cccgcttcac ccatgtggta accgctgata
     5641 ctgatggtat taaatcgcgg catgttgccg gaacaccagg cgatgatgtc ggcgataatg
     5701 cgcattgacg gttttggcgg gtaaatatag gtgttgcggc agaggtactc tttgaggata
     5761 tcgttctgaa tggtgccggt cagtttatca ggtgtaacac cttgctcttc cgcggcgacg
     5821 atataaaacg ccagtactgg tagcactgcg ccattcatgg tcatcgaaac cgacatttta
     5881 tccagcggga tctggtcgaa caggactttc atatcttcca cggtgtcgat agcgacgccc
     5941 gctttgccga cgtcgcccgc cacgcgcggg ttatcggagt cgtagcctcg gtgggtggca
     6001 aggtcaaacg caacggaaag acctttttgc ccggcggcca ggttacggcg ataaaaagcg
     6061 ttggactctt ttgctgttga aaaaccagca tactgacgga tggtccacgg ttgggcggta
     6121 tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac caggaagggt acctgtcacc
     6181 tccagattat cgagatcggc ttcggtatac agcggcttga tggtgatccc ttccgcggtt
     6241 tgctgaacca gcgagtcgac agttttctcc cgacggctca attccttgtt ggcaagctgt
     6301 tgccactcct gcacgttaga cattatttgc tccgtaaaac gcaatcaaaa tgagggtatt
     6361 tgcgcctacg gtgagctttt tacgtgggtt tgtcgccagc aaaaaaaatg cattcagacg
     6421 tcgttttttg ccgcctgaga tttgtctatt ttgtgatgtg attcaaggaa tacaaaaaga
     6481 agtgaggaca acgcctgata tgtgctatca ggcattattt gatggattaa tcctgacgaa
     6541 ggactttgtg accataatcg agcaacgcat cagtgacttt acgcatcatg cggctttcag
     6601 gagcaaagcg gtgccagtag agcatccggc gttgaaatag ccctggcgtt aagtcaatca
     6661 gttcaccgct ggctagttct ttctcgattt gcaggtgcgg gatcatacag caggtggtgc
     6721 cctggcgagc aagttgcacg aacgcttctg aagaattaac gatatggcag ggcacgctgc
     6781 ctggaggcag atcgaagttt tgctgcaaaa aggcctggtg catatcgtca agatggtcaa
     6841 aggcgaccac aggtgctttc aataatgccg aacgcgttac gccgttaggg aaatattttt
     6901 cggcaaacgg ttttgagctg acgaacagat agtcaagcgc accgagctta tcgacaaggc
     6961 aactcggcag cgcctgatgt tgaatactca ccgcgccgac cacttcgccg cggcgcagac
     7021 gttcctgagt acgggtttca tcttctacct gcaagttgag gcggataggc gaatcagcca
     7081 atacaggagc cagtgccgga agcaaccacg tcgccagact gtcggcgttg accgccagtg
     7141 aaagcagcag cggagtcgaa ccggtttgtt catcgcccag ccactcttct tccagcaact
     7201 ccacctggcg cagcagtgcc agcagttttt gcccttgttc cgtcgggcgc ggcggtacgg
     7261 tacgcaccaa cagcggctgc ccgaacatat tttccagttg cttaatgcgc tgtgagacgg
     7321 ctgattgtgt aatgcacagc ttttgtgccg cgcgctcaaa tcctcgttca cgtatcaccg
     7381 catccagtgc ctgtaatgtt ctgtagtccg ggcgtttcat tgctgttgtc tgctcctggt
     7441 aaatgctttt tcctgcacta tgacacaatt ttgtgtcagg ttgcatatac cgcagccgtc
     7501 ggcgggtaac atttgtatca atggttaaac gcgcagctta aaagatcagg gatttgcgct
     7561 attgcatttc gcttagcgac ttcgcgccct ggttatgttg aatggcgtgg cgttattgcc
     7621 tctgttcgca ccgtggagcg gcatacccag accgttgtat gctttatttg cggggcgaaa
     7681 ggggatgccc gccattgcgc gatattttat ggatgagtta accacccggc ttgccaacgg
     7741 ggtctgaatc gctttttttg tatataatgc gtgtgaaatt tcataccaca ggcgaaacga
     7801 tcatgatgca ggatgaattg aaaaaagcag taggatgggc ggcacttcag tatgttcagc
     7861 ccggcaccat tgttggtgta ggtacaggtt ccaccgccgc acactttatt gacgcgctcg
     7921 gcacgatgaa aggccagatt gaaggggccg tttccagttc agatgcttcc actgaaaaac
     7981 tgaaaagcct cggcattcac gtttttgatc tcaacgaagt cgacagcctt ggcatctacg
     8041 ttgatggcgc agatgaaatc aacggccaca tgcaaatgat caaaggcggc ggcgcggcgc
     8101 tgacccgtga aaaaatcatt gcttcggttg cggaaaaatt tatctgtatt gccgacgcct
     8161 ccaagcaggt tgatattctg ggtaaattcc cgctgccagt agaagttatc ccgatggcac
     8221 gtagtgcagt ggcgcgccag ctggtgaaac tgggcggtcg tccggaatac cgtcagggcg
     8281 tggtgaccga taatggcaac gtgatcctcg acgtccacgg catggaaatc cttgacccga
     8341 tagcgatgga aaacgccata aatgcgattc ctggcgtggt gactgttggc ttgtttgcta
     8401 accgtggcgc ggacgttgcg ctgattggca cacctgacgg tgtcaaaacc attgtgaaat
     8461 gatctgacgg gggaacctcc cccgttaaaa aaattctctt cattaaattt ggtgacatgt
     8521 gtcacgtttt tacctggcta ttgtcgattg ctctaaataa atcctctaaa ccagcatatt
     8581 caaccaagaa atacctttgc gtgatatttc ctcaacatcg cgacgcaaac gttcatattg
     8641 ccgcaatatt attttttgat atgttgaaag gcggatgcaa atccgcacac aacatttcaa
     8701 agacaggatt gggtaaatgg caaaggtatc gctggagaaa gacaagatta agtttctgct
     8761 ggtagaaggc gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt acaccaacat
     8821 cgaatttcac aaaggcgcgc tggatgatga acaattaaaa gaatccatcc gcgatgccca
     8881 cttcatcggc ctgcgatccc gtacccatct gactgaagac gtgatcaacg ccgcagaaaa
     8941 gctggtcgct attggctgtt tctgtatagg aacaaaccag gttgatctgg atgcggcggc
     9001 aaagcgcggg atcccggtat ttaacgcacc gttctcaaat acgcgctctg ttgcggagct
     9061 ggtgattggc gaactgctgc tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca
     9121 ccgtggcgtg tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg
     9181 tatcatcggc tacggtcata ttggtacgca attgggcatt ctggctgaat cgctgggaat
     9241 gtatgtttac ttttatgata ttgaaaataa actgccgctg ggcaacgcca ctcaggtaca
     9301 gcatctttct gacctgctga atatgagcga tgtggtgagt ctgcatgtac cagagaatcc
     9361 gtccaccaaa aatatgatgg gcgcgaaaga aatttcacta atgaagcccg gctcgctgct
     9421 gattaatgct tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag
     9481 caaacatctg gcgggggcgg caatcgacgt attcccgacg gaaccggcga ccaatagcga
     9541 tccatttacc tctccactgt gtgaattcga caacgtactt ctgacgccac acattggcgg
     9601 ttcgactcag gaagcgcagg agaatatcgg cctggaagtt gcgggtaaat tgatcaagta
     9661 ttctgacaat ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc tgccactgca
     9721 tggtggtcgt cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa
     9781 caaaatcttc gccgagcagg gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca
     9841 gatgggttat gtagttattg atattgaagc tgacgaagac gttgccgaaa aagcgctgca
     9901 ggcaatgaaa gctattccgg gtaccattcg cgcccgtctg ctgtactaat tccccttctc
     9961 tgaaaattaa cgggcaggtc actgacttgc ccgttttttt atcccttctc cacaccgttg
    10021 ccgcgttgcg cattctactt tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat
    10081 gcgttgtgtt tatttaatgg ggatttacga tggcgggaca gtatggggct gtaacggtaa
    10141 agccctcacc gaagcgaggg cttaaaggag aagggttatg atgcgacttg tcatcatact
    10201 gattgtactg ttactgataa gtttcggagc ttattaacag tcagtctctg gggaggagtc
    10261 atcctccctt acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg
    10321 atgcggggtg catcgccctg aaccgaactt tttgatagcc acttcatcgc ccacgtttac
    10381 tgccgtaata gatccttttt cccaaaaatt atctctgttt tgcatggggt ttttcgaaca
    10441 cgctcgaaga aaagatttaa aggtagatca atgcgttgtg gttatttaaa gagagcttac
    10501 gatagctggg cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg
    10561 agaagggtta tgatgcgact tgccatcata ctgattgtac tgttactcat aagtttccca
    10621 gcttattaac agtcagtcac aggggaggag caatcctccc ttacccttac tcactaaatt
    10681 aggttaaaga atcaacgatg tcaatcaggg cgatgcaggt gtatcgccct taccactccc
    10741 agactttcga cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt
    10801 tttccaccaa ctgacaatca tgcgcataac ccaccggttg cgttttatag tgctgccagt
    10861 tttgtaaggt ccgatcataa aaaccaccgc ccattcccag gcgctgaccg tactcatcaa
    10921 aggcgaccag cggtgtgatc agcacgtcta atcgggaaag gggtagcacg tcacgcacat
    10981 ccagttttgg ctcatggatt ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat
    11041 tcaggaacag caaattacca gcactaaagg gatgcaaaac tggaagatat acgcgcttac
    11101 cggcgcgcca gagttgttct attagcggct gggtgtcgag ttcaccatca aaagagagga
    11161 atacagcgac cgtatgcgcc atcaccaccg ggggataagt catcatccgg gtagcggctt
    11221 gttgacccat ttcctgctgt tgttccggcg ttaacgcacg acgacgttgc cgaatcattt
    11281 tgcggatttc ttgtcgggat aatgtcagtg ggagttctgg tagttgcgtc atggctggtg
    11341 ccagataaga agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt
    11401 caaggtgaat gtgtcgtcgc agttttaagg cttctcggac ggaccgagca tgctcaccaa
    11461 ccgcggagcg ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg
    11521 aacatctcag agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca
    11581 ttcaaagttt tggttagttt tttcggtgat gcgaccttgt tcaagtaacg cttgttctat
    11641 ggtctgctgc agcatccgaa tacgctgttc catacttgcc gcgtagtcac gagtcttcgc
    11701 tttttcttgc gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt
    11761 atttgtgact ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc
    11821 ctgattcaac gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat
    11881 ttggatatcg acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc
    11941 gtctttagac ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca
    12001 agcccttttc tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt
    12061 gacgacgaat gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt
    12121 atctgaacca acaagggacg ggtctgaccc ctgccgagat gcatggttta atcagtggga
    12181 tgatatgtgg cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg
    12241 aaggcatggc tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca
    12301 gcgatgccct gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg
    12361 tcagcgtttt cgatcgggct gatgcattgg caggttgggt caatcacttc ctgcttggtc
    12421 ttggcgttac gcaaccgaag ctggataaag tgaccggcga aaccggtgaa gctatcgacg
    12481 atctgcgtaa cattgcgcaa ctgggttacg acgaagacga agatcaggaa gagcttgaaa
    12541 tgtcgcttga agagatcatc gaatacgttc gtgttgccgc gctgttatgc cacgacacct
    12601 ttactcatcc gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa
    12661 aaacgtaagg agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag
    12721 gccctggtgg agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta
    12781 acacgtagcg ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc
    12841 ggctttaacg aaccggaagc ggtgctggtg ctgattaaaa gcgatgacac tcataaccac
    12901 agcgttctgt ttaaccgcgt tcgcgacctg acggcggaga tctggtttgg ccgtcgctta
    12961 ggccaggatg ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatc
    13021 aatcagcaac tttatcaact acttaacggc ctggatgtgg tttaccatgc ccagggcgaa
    13081 tatgcatatg ctgatgaaat catgaacagt gcgctggaaa aactgcgtaa aggttcgcgg
    13141 caaaatctca ccgcaccggc aacgatgatc gactggcgtc ctgttgttca tgaaatgcgc
    13201 ctgttcaaat cgccagaaga gattgccgta ctccgccgcg cgggagaaat caccgccatg
    13261 gcacatacac gggcgatgga aaaatgccgt ccgggaatgt tcgagtacca tctggaaggc
    13321 gaaattcacc acgaatttaa ccgccacggt gcgcgctatc cgtcctataa caccattgtc
    13381 ggcagcggtg aaaacggctg cattctgcac tacaccgaaa acgagagtga actgcgcgac
    13441 ggtgacctgg tgttgattga cgcgggttgt gaatacaaag gttacgcggg cgatattacc
    13501 cgcaccttcc cggtcaacgg caaattcacc caggcccagc gtgaaatcta cgacattgtg
    13561 ctggagtctc tcgaaaccag cctgcgcctg tatcgtccgg gaacttccat tctggaagtc
    13621 actggtgaag tggtgcgcat catggttagc ggcctggtaa aactcggcat tctgaaaggt
    13681 gatgttgatg aactgatcgc tcagaatgcc catcgtcctt tctttatgca tggccttagc
    13741 cactggttag ggctagatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt
    13801 ctggaaccgg gcatggtact gaccgtagag ccagggctgt atattgcgcc ggatgcagaa
    13861 gtgccagaac aatatcgcgg tatcggcatt cgtattgaag acgacattgt gattaccgaa
    13921 accggtaacg aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg
    13981 atggctgctg cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga
    14041 cgctggcgct ggctatttcc cggttaagtc acggggcgct gccggtacat ttgattgaag
    14101 cgactgcgcc agagtcacat gctcatccgg gctttgatgg acgagcgata gcgctggcgg
    14161 cgggtacctg tcagcaactg gcgcgcatcg gcgtctggca atctctggcg gattgcgcaa
    14221 ctgccatcac caccgtgcat gtcagcgatc gtggtcacgc tggatttgtc accctcgccg
    14281 cagaagatta ccaactggcg gcgctgggac aggttgtcga attgcacaat gtcgggcaac
    14341 ggctgtttgc attgctgcgt aaagcacctg gcgtaacgct gcattgccct gatcgcgtgg
    14401 ctaacgttgc ccgtactcag agtcacgttg aagtgacgct ggagagtggc gagacgctga
    14461 cgggccgcgt gctggtagca gctgatggca cccattcagc gttagccacc gcgtgcggcg
    14521 ttgactggca gcaggagcct tacgaacaac tggccgtgat tgccaacgtt gcgacttccg
    14581 ttgcgcacga aggccgcgct tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc
    14641 cgatgtctga cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg
    14701 tgctgtcgtg gagcgacgag aagttttgcc gtgaactcca gtcggccttt ggctggcgac
    14761 ttggaaaaat tacccacgcc ggtaaacgca gtgcttatcc gctggcgtta acccgcgccg
    14821 ccagagctat tacccatcgt accgtgctgg tgggcaatgc ggcgcaaact ctgcacccga
    14881 ttgccgggca agggtttaac ctcggtatgc gagatgtcat gagccttgcg gaaaccctga
    14941 ctcaggcgca tgagcgcgga gaagacatcg gtgattacgg catattgtgc cgctatcagc
    15001 agcgtcgaca gagcgatcgc gaagcaacca tcggcgtcac ggacagcctg gtacatcttt
    15061 ttgccaaccg ttgggcaccg ctggttgtcg ggcgcaacat cggactgatg acgatggaat
    15121 tattcacccc ggcacgcgat gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa
    15181 ggcggtttta aggaataaac aatgcaatgt gttgatgtag ccattgttgg tggcggcatg
    15241 gtggggctgg cggttgcctg cggcttacag gggagcggct tacgcgttgc cgtactggag
    15301 cagcgcgtac cggaacctct ggcggcggat gcaccaccac aactgcgcgt ttcggctatc
    15361 aatgccgcca gcgaaaaatt actcacccgt cttggcgtct ggcaggacat tctctctcgt
    15421 agggccagtt gttatcacgg tatggaagtg tgggacaaag acagttttgg tcacatttcg
    15481 tttgacgatc aaagcatggg ctatagccat cttgggcata tcgttgaaaa ctcagtgatt
    15541 cactacgcgc tgtggaacaa agcgcagcag tcgtcagata tcactctgtt ggcccccgca
    15601 gaattacagc aggtcgcctg gggagaaaat gaaactttcc tgacgctgaa agatggcagc
    15661 atgttaacgg cgcgtctggt gattggcgcg gacggtgcta attcctggtt gcgcaacaaa
    15721 gcagatattt cgctgacttt ctgggattat cagcatcacg cgctggtagc gaccattcgc
    15781 acggaagaac cgcatgatgc ggtggcgcgg caggttttcc atggcgaagg cattctggcc
    15841 tttttaccgc ttagcgatcc gcatctttgc tcaattgtct ggtcactgtc gccagaggaa
    15901 gcgcagcgga tgcagcaggc aagtgaagat gaatttaatc gcgcgttaaa tatcgctttt
    15961 gataatcgcc tgggcttatg caaggttgag agcgcgcgtc aggtgttccc actgacgggg
    16021 cgttatgcgc gccagtttgc cgcgcaccgt ctggtgctag tgggcgacgc cgcgcatacc
    16081 attcacccgc tggcggggca gggagtaaat ctcggcttta tggatgctgc agagctgatt
    16141 gctgaactga aacggttgca tcgtcagggg aaagacatcg ggcagtacat ttacctgcgc
    16201 cggtatgagc gtagccgcaa gcacagtgcg gcgctgatgc tggctggtat gcagggattc
    16261 cgcgatctgt tttccggtac caatccggcg aaaaaactgc tgcgtgatat tggtttgaaa
    16321 ctggccgaca cgcttcctgg cgttaaacca caacttatcc gccaggcaat gggattaaac
    16381 gatttgcctg aatggctgcg ttaaaaattt ctcttctgct gtttatttga tacccatcac
    16441 actttcatct cccggttttt tcgccgggag attttcctca tttgaaataa actaatttca
    16501 cctccgtttt cgcattatat tttctaatgt cattattttt tgatttagtg ttttttgaca
    16561 tttttttagc tcttaatatt gccttattca aattagcttt ctaatggcat catctttata
    16621 tagaaactgg tgtgtttttt ggttttttat tctgtcgcga ttttggcgtt ttttaaccat
    16681 aagctaatgt gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca
    16741 ggcaaaagag aacgattgcg ttggggaccg ggagtggctc cgatgctggg tttcgtggtg
    16801 ataatttcac catgaaaagt tgtcagcccc gcttattcaa tgaggacaag atggcacaac
    16861 agactccttt gtacgaacaa cacacgcttt gcggcgctcg catggtggat ttccacggct
    16921 ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta cgtaccgatg
    16981 ccggaatgtt tgatgtgtca catatgacca tcgtcgatct ccgcggcagc cgcacccggg
    17041 agtttctgcg ttatctgctg gcgaacgatg tggcgaagct caccaaaagc ggcaaagccc
    17101 tttactcggg gatgttgaat gcctctggcg gtgtgataga tgacctcatc gtctactact
    17161 ttactgaaga tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa gacctctcct
    17221 ggattaccca acacgctgaa cctttcggca tcgaaattac cgttcgtgat gacctttcca
    17281 tgattgccgt gcaagggccg aatgcgcagg caaaagctgc cacactgttt aatgacgccc
    17341 agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt gcaggcgggc gatctgttta
    17401 ttgccaccac tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc aatgaaaaag
    17461 cggccgattt ctggcgtgcg ctggtggaag cgggtgttaa gccatgtggc ttgggcgcgc
    17521 gtgacacgct gcgtctggaa gcgggcatga atctttatgg tcaggagatg gacgaaacta
    17581 tttctccttt agccgccaac atgggctgga ctatcgcctg ggaaccggca gatcgtgact
    17641 ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa ctggttggtc
    17701 tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt accgatgcgc
    17761 agggcaacca gcatgaaggc attatcacca gcggtacttt ctccccgacg ctgggttaca
    17821 gcattgcgct ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg caaattcgca
    17881 accgtgaaat gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc aaagccgtcg
    17941 cgtgatttac ttttttggag attgattgat gagcaacgta ccagcagaac tgaaatacag
    18001 caaagaacac gaatggctgc gtaaagaagc cgacggcact tacaccgttg gtattaccga
    18061 acatgctcag gagctgttag gcgatatggt gtttgttgac ctgccggaag tgggcgcaac
    18121 ggttagcgcg ggcgatgact gcgcggttgc cgaatcagta aaagcggcgt cagacattta
    18181 tgcgccagta agcggtgaaa tcgtggcggt aaacgacgca ctgagcgatt ccccggaact
    18241 ggtgaacagc gaaccgtatg caggtggctg gatctttaaa atcaaagcca gcgatgaaag
    18301 cgaactggaa tcactgctgg atgcgaccgc atacgaagca ttgttagaag acgagtaacg
    18361 gctttattcc tcttctgcgg gagaggatca gggtgaggaa aatttatgcc tcaccctcac
    18421 tctcttcgta aggagagagg ttcacaattc actgcacgtt tcaggaacca tcgctcatga
    18481 cacagacgtt aagccagctt gaaaacagcg gcgcttttat tgaacgccat atcggaccgg
    18541 acgccgcgca acagcaagaa atgctgaatg ccgttggcgc acaatcgtta aacgcgctga
    18601 ccggccagat tgtaccgaaa gatattcagc ttgcgacccc gccgcaggtt ggcgcaccgg
    18661 cgaccgaata cgccgcactg gcagaactca aggctattgc cagtcgcaat aaacgcttca
    18721 cgtcttacat cggcatgggt tacaccgccg tgcagctacc gccggttatc ctgcgtaaca
    18781 tgctggaaaa tccgggctgg tataccgcgt acactccgta tcaacctgaa gtctcccagg
    18841 gccgccttga agcactgctc aacttccagc aggtaacgct ggatttgact ggactggata
    18901 tggcctctgc ttctcttctg gacgaggcca ccgctgccgc cgaagcaatg gcgatggcga
    18961 aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt ggcttccgat gtgcatccgc
    19021 aaacgctgga tgtggtccgt actcgtgccg aaacctttgg ttttgaagtg attgtcgatg
    19081 acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt gctgttacag caggtaggca
    19141 ctaccggtga aattcacgac tacactgcgc ttattagcga actgaaatca cgcaaaattg
    19201 tggtcagcgt tgccgccgat attatggcgc tggtgctgtt aactgcgccg ggtaaacagg
    19261 gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt gccgatgggc tacggtggcc
    19321 cacacgcggc attctttgcg gcgaaagatg aatacaaacg ctcaatgccg ggccgtatta
    19381 tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg catggcgatg cagactcgcg
    19441 agcaacatat ccgccgtgag aaagcgaact ccaacatttg tacttcccag gtactgctgg
    19501 caaacatcgc cagcctgtat gccgtttatc acggcccggt tggcctgaaa cgtatcgcta
    19561 accgcattca ccgtttgacc gatatcctgg cggcaggcct gcaacaaaaa ggtctgaagc
    19621 tgcgccatgc gcactatttc gacactctgt gtgtggaagt ggccgacaag gcgggcgtac
    19681 tggcgcgtgc cgaagcggct gaaatcaacc tgcgtagcga tatcctgaac gcggttggga
    19741 tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca gcttttcagc gtgctgctgg
    19801 gcgataacca cgggctggag atcgacacgc tggacaaaga cgtggctcac gacagccgct
    19861 ctattcagcc tgcgatgctg cgcgacgacg aaatcctcac tcatccggtg tttaatcgct
    19921 accacagcga aaccgaaatg atgcgctata tgcactcgct ggagcgtaaa gatctggcgc
    19981 tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa actgaacgcc gccgccgaga
    20041 tgatcccaat cacctggccg gaatttgccg aactgcaccc gttttgcccg ccggaacagg
    20101 ctgaaggtta tcagcagatg attgcgcagc tggctgactg gctggtgaaa ctgaccggtt
    20161 acgacgccgt ttgtatgcag ccgaactccg gcgcacaggg cgaatacgcg ggcctgctgg
    20221 cgattcgtca ttatcatgaa agccgcaacg aagggcatcg cgatatctgc ctgatcccgg
    20281 cttctgcgca cggaactaac cccgcttctg cacatatggc aggaatgcag gtggtggttg
    20341 tggcgtgtga taaaaacggc aacatcgatc tgactgatct gcgcgcgaaa gcggaacagg
    20401 cgggcgataa cctctcctgt atcatggtga cttatccctc tacccacggc gtgtatgaag
    20461 aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg cggtcaggtt taccttgatg
    20521 gcgcgaacat gaacgcccag gttggcatca cctcgccggg ctttattggc gcggacgttt
    20581 cgcacctcaa cctgcataaa actttctgca ttccgcacgg cggtggtggt ccgggtatgg
    20641 gaccgatcgg tgtgaaagcg catctggcac cgtttgtacc gggtcatagc gtggtgcaaa
    20701 tcgaaggcat gttaacccgt cagggcgcag tttctgcggc accgttcggt agcgcctcta
    20761 tcctgccaat cagctggatg tacatccgca tgatgggcgc agaagggctg aaaaaagcaa
    20821 gccaggtggc aatcctcaac gccaactata ttgccagccg cctgcaggat gccttcccgg
    20881 tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg tattctcgat attcgcccgc
    20941 tgaaagaaga aaccagcatc agcgagctgg atattgccaa gcgcctgatc gactacggtt
    21001 tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct gatggttgaa ccgactgaat
    21061 ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat gctggctatc cgcgcagaaa
    21121 ttgaccaggt gaaagccggt gtctggccgc tggaagataa cccgctggtg aacgcgccac
    21181 acattcagag cgaactggtc gccgagtggg cgcatccgta cagccgtgaa gttgcggtat
    21241 tcccggcagg cgtggcagac aaatactggc cgacggtgaa acgtctggat gatgtttacg
    21301 gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga ataccagtaa ttcactgatt
    21361 cgactatttt ctaaaggcgc ttcggcgcct ttttagtcag atgacaaagt acaaaagtgc
    21421 tcagacagtc ccctcgcccc tttggggaga gggttagggt gaggggaaca ggccggcact
    21481 ggcgcgaata tttaccctca ccccggccct ctccctgaaa gggcgagggg aaaagcgtgc
    21541 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    21601 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    21661 gcaactgcat tactgttggc gaaagaagag tatacggtgg cggttaatta tcaacaaaac
    21721 ctccatgcgg cgcaggaagt ggtgaactta atcacgcaag ccggtggcaa ggcattcgtg
    21781 ctccaggcgg atatcagcga cgaaaatcag gtcatcgcaa tgtttacagc aatcgatcag
    21841 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    21901 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    21961 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    22021 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga ataggttgat
    22081 tacgcggcat cgaaaggggc gattgatacg ttaaccaccg gactatcgct ggaagtcgcc
    22141 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    22201 agcggcggcg agcttggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    22261 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    22321 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    22381 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    22441 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    22501 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    22561 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    22621 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    22681 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    22741 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    22801 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    22861 atcgcatcgc cggtgccgcc ttcggccttc actgcgttga tcatgtaata gctgaaacca
    22921 agataatcgc aggtgccttc acgcagtaca tccagatcgc catcttccat tttgatgtta
    22981 aatccgcgac gctcccactc gttcaacaca taggacgggt aatagccgcg tagctgcaca
    23041 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    23101 caggagtaag gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    23161 atgcgacgcg cggctttcac tgctagggcg ctggcgacaa actggtgatg cagcacctgg
    23221 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    23281 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    23341 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgact
    23401 ttacggttgg tccagctacc gtattgctgc accagatgca gcggtatttc aaagtgggag
    23461 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    23521 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    23581 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    23641 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    23701 gtgatttcgc gcggcacgcc gtgtgcgcca ccggtcagaa cgtcacaaat gctcggtcct
    23761 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    23821 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatgagt caattcttac
    23881 aggaaatagt accatgcagc caaacgacat cactttttct caacgtttcc aggatgacat
    23941 tctggctggg cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg
    24001 tgatgtgctt cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac
    24061 cgcaacctca accgtaacgc tggatacgct gacggaaaaa catgcagagc aggaaaatat
    24121 gaccctgact gaactgataa aggtcattgc cgacatctat cccggtcaga cacagtttta
    24181 tgtgattgaa tttaaatgtc tttaaattga actcacgcct gttagttgaa atttctcatt
    24241 taatttcatg atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt
    24301 tcactggaac tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctccgg
    24361 agtaagttat ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg
    24421 ccaacagcgt cagtcacggc attgggttgg tgtttggtat cgttgggctg gtgttgctac
    24481 tggttcaggc ggtggatctc aatgccagcg ccacagcgat aaccagctac agcctctatg
    24541 gcggcagtat gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac
    24601 gggcaaaaat gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa
    24661 cctacacgcc gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg
    24721 ttatctggag cctggcattg ctggggattc tgtttaaact gaccatcgcg caccgattca
    24781 aaattttatc tctggtgacc tatctggcga tgggctggct gtcgctggtg gtaatttatg
    24841 aaatggcagt taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt
    24901 attcgctcgg agtgattttc tatgtctgca aacgcattcc ctacaatcat gccatctggc
    24961 acggcttcgt gctcggcggc agtatgtgcc actttctggc gatctatttg tatattgggc
    25021 aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg ccagcgtcgc atcaggcatc
    25081 ggagcgcaac cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt
    25141 tgtgataaag gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata
    25201 aatgcctgat gcgatacaga gatttactct tcgagtgaat acggcagcgg ctcgatatgc
    25261 agtgtattcg catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg
    25321 acgacctgta ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta
    25381 cgacgccagt tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg
    25441 gcgctacctg ccagcaacca gagcgcacgt ttattggcac cacggaattt tgctcgcgcc
    25501 accatctctt gtccggtata acagcctttc ttaaaactga taccgcccag cgcctggaga
    25561 ttggtcgcct gtgggataaa ctgcccgctg ttcgcggcat caatcaccgg gaaacccgct
    25621 tcaatgttta atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta
    25681 tcggtcagca tattggcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt
    25741 tcaaaccata gcaaagtggt cgcgccttct ttaactacct gtttttctct cgaaggtagt
    25801 tcactaaaga gattggcgag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca
    25861 cgctcgtcgt ctgccgcgat ggtcacttta gagaacaccg catatttttt cagttcagtc
    25921 agctgcggtt cacgcacgct gcgccgttca atccacgcaa agccatcgcc gtcgcggaac
    25981 agacgtaaat tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga
    26041 ttttctgtca tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc
    26101 gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg
    26161 gcagaagccg taggctgacg gggaggaaaa ggtgtaaaag ccataataga gtcctgagtt
    26221 gcttaacggc gtattaataa ctaatggtaa aagagctatg aggcaatgca agtgctttca
    26281 ctacgcgatc agtgccttct gcgaggcgac ttccaaaaaa atgaagctct tgaattgttg
    26341 tgaattaatt tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa
    26401 cacgttacaa tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa
    26461 caaagcccgc attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat
    26521 gccgtttttc gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg
    26581 tctgctggaa tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc
    26641 cgatgcagaa ctggaaatga tggtccgact catccagaca cggaaccggg aacgtggtcc
    26701 tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg
    26761 ggctggttgc cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga
    26821 tggtgttact ttcgctggtg gtatttgatt gcgttcgcag ccagcggcgt attaatgctc
    26881 gccaggggga aattcgattg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga
    26941 gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg
    27001 gcggtaaacg gcaacattta tggctggcag ccgacagcat ggacgaagct gaatggcggg
    27061 atttacggcg gattttgttg caacaagaga cgcaaagata aatatgacgg caaagcatat
    27121 gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg
    27181 caccagctct gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga
    27241 cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc
    27301 gttggccagt agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg
    27361 agcgcatcga ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaaca
    27421 attttacctt caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg
    27481 atttcgccaa aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat
    27541 ttcggggagt cgtccttgag gttatgtaag gtcaccagtt ctgggccgag aatatcgcgg
    27601 attttttctg ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc
    27661 ataacgtaag cttctcgatt ctgctgtggc ttttgcggct agtgtatcag aatcgcttta
    27721 gtcacggcac aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac
    27781 aggatctggc acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg
    27841 aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc
    27901 gcgggttgac gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc
    27961 tagagggcgg gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt
    28021 tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac
    28081 cgaaattgcc acagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac
    28141 aggcaccatt aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt
    28201 atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctac
    28261 gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttaggtg
    28321 aagaggccgt ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgttgaatg
    28381 gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct
    28441 tctggcaccg cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac
    28501 cgcatgtgtt gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg
    28561 tggtgcagat gctactgggc cacagcgatc tctccaccac gcaaatttat acgcatgtcg
    28621 ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa
    28681 gaacgggaag atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag
    28741 gctttgctca ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcattaaaa
    28801 gcagcgatat tcagccagca cccgtggctg gcatgaagac tgtgctgact aacagcggtg
    28861 tgttgtacat caccgatgac ggtaaacata tcattcaggg gccaatgtat gacgttagtg
    28921 gcgcggctcc ggtcaatgtc accaataaaa tgctgctaaa gcagttgaat gcgcttgaaa
    28981 aagagatgat cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata
    29041 ttacctgtgg gtactgccac aaactgcatg agcaaatggc agactacaac gcgctgggga
    29101 tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga gagccaggct gagcaacaaa
    29161 tgaaagccat ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggctggta
    29221 aagctgccac tccggcaagt tgcgatatcg atattgccga tcattacgca cttggcgtcc
    29281 agcttggcgt tagcggcact ccggcaattg tgctgagcaa tggcacactt gttccgggtt
    29341 accagccgcc gaaagagatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta
    29401 attaattcgc gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga
    29461 cttgcccgct gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag
    29521 tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt
    29581 cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt
    29641 cggtgatttc gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc
    29701 gcttggttgc agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt
    29761 aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga
    29821 taacggtatt tcctcccatg cgggggttga acacgctcgc tcgttgggca ttccggttat
    29881 tgttaccgat caccatttgc caggcgaaac attacccgcg gcggaagcga tcattaaccc
    29941 taacttgcgc gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta
    30001 tctgatgctg gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat
    30061 cgcaattcct aacctggcag aactgctgga tctggtcgcg ctggggacag tggcggacgt
    30121 cgtgccgctg gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc
    30181 cggaaagtgc cgtccaggga ttaaagcgct gctggaagtg gcaaaccgtg atgcacaaaa
    30241 actcgccgcc agcgatttag gttttgcgct ggggccacgt ctcaatgctg ccgggcgact
    30301 ggacgatatg tccgtcggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgc
    30361 gctggcaaat gaactcgatg cgctaaatca gacgcgaaaa gagatcgaac aaggaatgca
    30421 gattgaagcc ttgaccctgt gcgagaaact ggagcgaagt cgcgacacgc tacccggcgg
    30481 gctggcaatg tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgcat
    30541 caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca gcaggtgatg gtacgctgaa
    30601 aggttccggt cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac
    30661 actctacccc ggtatgatgc tgaagtttgg cggtcatgcg atggcggcgg gtttgtcgct
    30721 ggaagaggat aaattcgaac tctttcaaca acggtttggc gagctggtta ccgagtggct
    30781 ggacccttcg ctattgcaag gcgaagtggt gtcagacggc ccgttaagcc cggccgaaat
    30841 gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga
    30901 gccgctgttt gatggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt
    30961 gaaagtcatg gtcgaaccgg tcggcggcgg tccgctgctg gatggtattg cttttaatgt
    31021 cgataccgcc ctctggccgg ataacggcgt gcgcgaagtg caactggctt acaagctcga
    31081 tatcaacgag tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat
    31141 ttagcgtcat cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc
    31201 gcattttgac tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa
    31261 tcgcattcag gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg
    31321 ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg
    31381 aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca
    31441 ccctcgacca aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag
    31501 aagctgacga cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa
    31561 aactggcgca gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgct
    31621 acctcgatat tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg
    31681 agcgtatgta tttgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt
    31741 cggaaggtga agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt
    31801 acggctggct gcgtacagaa actggcgttc accgcctggt gcgtaagagc ccgtttgact
    31861 ccggcggtcg tcgccacacg tcgttcagct ccgcgtttgt ttatccggaa gttgatgatg
    31921 atattgatat cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg
    31981 cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg
    32041 ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa gaataaagac caggccatga
    32101 agcagatgaa agcgaagctt tatgaactgg agatgcagaa gaaaaatgct gagaaacagg
    32161 cgatggaaga taacaaatcc gacatcggct ggggcagcca gattcgttct tatgtccttg
    32221 atgactcccg cattaaagat ctgcgcaccg gggttgaaac ccgcaatacg caggccgtgc
    32281 tggacggcag cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca
    32341 acatgtctga acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga
    32401 aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt
    32461 tccgtcgcga tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag
    32521 aactggaagc gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta
    32581 tgggtaaagc ctcttttgtt accctgcagg acgttggcgg tcgtattcag ctgtacgttg
    32641 cccgtgacga tcttccggaa ggcgtttaca acgagcagtt caaaaaatgg gatctcggcg
    32701 acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact
    32761 gcaccgagct gcgtctgctg accaaagcac tgcgtccgct gccggacaaa ttccacggct
    32821 tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat
    32881 cccgcaacac ctttaaagtg cgttcgcaga tcctctctgg cattcgccag ttcatggtaa
    32941 atcgcggctt tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg
    33001 cgcgtccgtt tatcacccat cataacgcgc tggatcttga catgtacctg cgtatcgcgc
    33061 cggaactgta cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc
    33121 gtaacttccg taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac
    33181 tctatatggc ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta
    33241 ctctggcaca ggatattctc ggtaagacgg aagtcaccta cggcgacgtg acgctggact
    33301 tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa
    33361 ccgacatggc ggatctggac aacttcgact ctgcaaaagc gattgctgaa tctatcggca
    33421 tccacgttga gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg
    33481 cagaagcaca tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc
    33541 tggcgcgtcg taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg
    33601 gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcagcgct
    33661 tcctggatca ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgacgaag
    33721 attacgtcac cgcactggaa cacggcttac cgccgacagc aggtctggga attggtatcg
    33781 accgtatggt aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg
    33841 cgatgcgtcc agtaaaataa gcattacgtt atgctaataa ccccggcaaa tgtcggggtt
    33901 ttttaattgt gctgcgcgaa agcagacaac aattttcttg cttcactatt ggctgcctgc
    33961 attaccatcc acggactgaa cgcccacggc gtggcatcaa taccgtgtaa tacatctgct
    34021 aaatcacacc attgataatc catcacttca tcatcgttga tctgtaacgc actgttggtg
    34081 cgtgcggcaa ataccggaca cacttcattt tccacaatgc cattcggatc ggtggcgcga
    34141 tagcgaaagt caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg
    34201 caacggcgga tcactgcgtc ttcgttgctt tctcccagtt gtgggtgccc acaaaccgag
    34261 ttagtccaca cgccaggcca tgcttttttg ctcagtgcgc ggcgggtaac taataattgc
    34321 cccttggcat taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt
    34381 gcggcatact tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt
    34441 tccgtttgca taatttctca catgtaattc tgatcactca tcattataga taattgaaga
    34501 gtgaaatgat taaccaagtg agcgtttatc gacaaccgcc cgttttgagc ggatgccgac
    34561 aggtaaaaac catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg
    34621 caacacagtc agaaatgaca ggaatagaga gagtaaaaag caacagcaga ctgaatatag
    34681 cgcgcattaa aagatttata ttattctcca tgctcatttt tcttcagctt gcgcatcaga
    34741 agccacatac gtactgtcct gacttttaaa tctttatcag agacggattc cttcaccagg
    34801 tcagcatgat gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg
    34861 agcataatgc cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg
    34921 tgagagagcg tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa
    34981 cggttggtgg tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg
    35041 aacatcacta gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc
    35101 atttttggca ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg
    35161 ctatgaacgc gcgtcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag
    35221 ctgttaaacg tgccgcctat cattgtccct acgccatcga cacgatggcc cgcgaataat
    35281 atcgtgtgaa gagagtttac gaccgactat ttcacccagt gccaggaaca tccccatcga
    35341 ctcgataaac acgatgatta acacggcagt catggtcaga atggaaacgg gatcgaaaat
    35401 cggcatacca aacgacatcg gggtgacaat cgcaaaccac aaggcatcat gtagcccgga
    35461 taaattgact tcattcatca tccacgaaag taaaaagcca aatacaatcc ccagtaatac
    35521 ggcgacgttg gacataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac
    35581 ggcaaaggag atacctgaat aaacgggatt accatattgc ggattccctt tacctccggc
    35641 ggcccagtca atacccacct gaatgatgct aagtccgaca gaagtaataa ccacaccggt
    35701 aaccagtggc gggaataaag gcatcaagcg accgataagt ggcgctaata atgtggtgat
    35761 aaaacccgcg gcgatagtgg caccaaatat ccccagcagg ccaatatccg ggttcatccc
    35821 aatggctatc attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat
    35881 ccccataaag cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag
    35941 atccgagcta atgagcatcg caatagcttc ttttgagagg cccagtcggt caccaatcat
    36001 aagaggaaca gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat
    36061 cagctttcct ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga
    36121 tgagagaagt tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa
    36181 aaagcaaccg tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt
    36241 gcgaggaagg ggagttagag acaggaattg cggaggcaat cacataatcg tggttaaacc
    36301 acgaaaatga gaagagaacc tgacaatgga attatctgta attttctcat attgatattt
    36361 cattaatcat tctggcatgt cgtactctcg catttaatcg tttttatctg gatagcac
//
LOCUS       DADLTD010000015        36071 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLTD010000015 DADLTD010000000
VERSION     DADLTD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.015"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(50..1699)
                     /gene="actP"
                     /locus_tag="JN701_000588"
     CDS             complement(50..1699)
                     /gene="actP"
                     /locus_tag="JN701_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY7363126.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPLILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1696..2010)
                     /locus_tag="JN701_000589"
     CDS             complement(1696..2010)
                     /locus_tag="JN701_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY7363127.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2142..4101)
                     /gene="acs"
                     /locus_tag="JN701_000590"
                     /pseudo
     CDS             complement(2142..4101)
                     /gene="acs"
                     /locus_tag="JN701_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4494..5930
                     /gene="nrfA"
                     /locus_tag="JN701_000591"
     CDS             4494..5930
                     /gene="nrfA"
                     /locus_tag="JN701_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY7363128.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5975..6541
                     /gene="nrfB"
                     /locus_tag="JN701_000592"
     CDS             5975..6541
                     /gene="nrfB"
                     /locus_tag="JN701_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY7363129.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6538..7209
                     /gene="nrfC"
                     /locus_tag="JN701_000593"
     CDS             6538..7209
                     /gene="nrfC"
                     /locus_tag="JN701_000593"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY7363130.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7206..8162
                     /gene="nrfD"
                     /locus_tag="JN701_000594"
     CDS             7206..8162
                     /gene="nrfD"
                     /locus_tag="JN701_000594"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY7363131.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8182..9900
                     /locus_tag="JN701_000595"
     CDS             8182..9900
                     /locus_tag="JN701_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7363132.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9893..10276
                     /gene="nrfF"
                     /locus_tag="JN701_000596"
     CDS             9893..10276
                     /gene="nrfF"
                     /locus_tag="JN701_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY7363133.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10273..10869
                     /gene="nrfG"
                     /locus_tag="JN701_000597"
     CDS             10273..10869
                     /gene="nrfG"
                     /locus_tag="JN701_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY7363134.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11210..12523
                     /gene="gltP"
                     /locus_tag="JN701_000598"
     CDS             11210..12523
                     /gene="gltP"
                     /locus_tag="JN701_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY7363135.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWLLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12601..12855)
                     /locus_tag="JN701_000599"
                     /pseudo
     CDS             complement(12601..12855)
                     /locus_tag="JN701_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005118502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(12840..13289)
                     /locus_tag="JN701_000600"
     CDS             complement(12840..13289)
                     /locus_tag="JN701_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005045755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7363136.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPGSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLEKPRKIWRTTLPSMPKCCLV"
     gene            complement(13383..15530)
                     /gene="fdhF"
                     /locus_tag="JN701_000601"
     CDS             complement(13383..15530)
                     /gene="fdhF"
                     /locus_tag="JN701_000601"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15111..15113),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HAY7363137.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15728..17194)
                     /gene="mdtP"
                     /locus_tag="JN701_000602"
     CDS             complement(15728..17194)
                     /gene="mdtP"
                     /locus_tag="JN701_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY7363138.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17191..19218)
                     /gene="mdtO"
                     /locus_tag="JN701_000603"
     CDS             complement(17191..19218)
                     /gene="mdtO"
                     /locus_tag="JN701_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY7363139.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQ
                     HAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKPPSMVA
                     DAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVL
                     RFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMV
                     VTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGA
                     LGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEERALLI
                     ERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATALSCSP
                     QITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19218..20249)
                     /gene="mdtN"
                     /locus_tag="JN701_000604"
     CDS             complement(19218..20249)
                     /gene="mdtN"
                     /locus_tag="JN701_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY7363140.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20268..20543)
                     /locus_tag="JN701_000605"
     CDS             complement(20268..20543)
                     /locus_tag="JN701_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363141.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20752..22737)
                     /gene="yjcS"
                     /locus_tag="JN701_000606"
     CDS             complement(20752..22737)
                     /gene="yjcS"
                     /locus_tag="JN701_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HAY7363142.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSHRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVRGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVISLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            22990..23307
                     /locus_tag="JN701_000607"
     CDS             22990..23307
                     /locus_tag="JN701_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HAY7363143.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23376..23705
                     /gene="yjdP"
                     /locus_tag="JN701_000608"
     CDS             23376..23705
                     /gene="yjdP"
                     /locus_tag="JN701_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HAY7363144.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23852..24610)
                     /gene="phnP"
                     /locus_tag="JN701_000609"
     CDS             complement(23852..24610)
                     /gene="phnP"
                     /locus_tag="JN701_000609"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY7363145.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(24612..25061)
                     /gene="phnO"
                     /locus_tag="JN701_000610"
     CDS             complement(24612..25061)
                     /gene="phnO"
                     /locus_tag="JN701_000610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY7363146.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVGSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(25048..25605)
                     /gene="phnN"
                     /locus_tag="JN701_000611"
     CDS             complement(25048..25605)
                     /gene="phnN"
                     /locus_tag="JN701_000611"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY7363147.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(25605..26741)
                     /gene="phnM"
                     /locus_tag="JN701_000612"
     CDS             complement(25605..26741)
                     /gene="phnM"
                     /locus_tag="JN701_000612"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY7363148.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(26738..27418)
                     /gene="phnL"
                     /locus_tag="JN701_000613"
     CDS             complement(26738..27418)
                     /gene="phnL"
                     /locus_tag="JN701_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY7363149.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(27529..28287)
                     /gene="phnK"
                     /locus_tag="JN701_000614"
     CDS             complement(27529..28287)
                     /gene="phnK"
                     /locus_tag="JN701_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY7363150.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(28284..29129)
                     /gene="phnJ"
                     /locus_tag="JN701_000615"
     CDS             complement(28284..29129)
                     /gene="phnJ"
                     /locus_tag="JN701_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY7363151.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(29122..30180)
                     /gene="phnI"
                     /locus_tag="JN701_000616"
     CDS             complement(29122..30180)
                     /gene="phnI"
                     /locus_tag="JN701_000616"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY7363152.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            complement(30180..30764)
                     /gene="phnH"
                     /locus_tag="JN701_000617"
     CDS             complement(30180..30764)
                     /gene="phnH"
                     /locus_tag="JN701_000617"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY7363153.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLTDNDTPMWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(30761..31213)
                     /gene="phnG"
                     /locus_tag="JN701_000618"
     CDS             complement(30761..31213)
                     /gene="phnG"
                     /locus_tag="JN701_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY7363154.1"
                     /translation="MHADIAPRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(31214..31939)
                     /gene="phnF"
                     /locus_tag="JN701_000619"
     CDS             complement(31214..31939)
                     /gene="phnF"
                     /locus_tag="JN701_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY7363155.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(31960..32739)
                     /gene="phnE"
                     /locus_tag="JN701_000620"
     CDS             complement(31960..32739)
                     /gene="phnE"
                     /locus_tag="JN701_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY7363156.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(32865..33881)
                     /gene="phnD"
                     /locus_tag="JN701_000621"
     CDS             complement(32865..33881)
                     /gene="phnD"
                     /locus_tag="JN701_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7363157.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(33906..34694)
                     /gene="phnC"
                     /locus_tag="JN701_000622"
     CDS             complement(33906..34694)
                     /gene="phnC"
                     /locus_tag="JN701_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY7363158.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(34827..35270)
                     /gene="yjdN"
                     /locus_tag="JN701_000623"
     CDS             complement(34827..35270)
                     /gene="yjdN"
                     /locus_tag="JN701_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY7363159.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(35430..35765)
                     /locus_tag="JN701_000624"
     CDS             complement(35430..35765)
                     /locus_tag="JN701_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY7363160.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         7796 a         9673 c        10191 g         8411 t
ORIGIN      
        1 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
       61 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac
      121 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
      181 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga
      241 ggatctgtac ccaaatagtc gggccgagga tcatcagcac cactgcggta atcaacccca
      301 gccagccgcc cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
      361 tgagcgggaa gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt
      421 tctgattctc aaacagcatg ccgaggataa tcgcaatcac acccaaaatc aggacggtga
      481 ttttcgatac ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca
      541 agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag
      601 tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
      661 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
      721 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
      781 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
      841 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
      901 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
      961 tcgggtgtac cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca
     1021 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca
     1081 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1141 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta
     1201 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     1261 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt
     1321 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     1381 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg
     1441 cggcggacat atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc
     1501 ctgccgtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     1561 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     1621 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     1681 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     1741 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     1801 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     1861 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     1921 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     1981 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2041 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2101 aggccggata aggcgtttac gccgcatccg gcaattgtgg gttacgatgg catcgcgata
     2161 gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg
     2221 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     2281 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     2341 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     2401 tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     2461 acggcggctt cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc
     2521 agacggtgac cggagacgtt cagcacgtcg tccacacgcc cggtgatcca gtaatagcca
     2581 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag
     2641 gtctgttcaa aacgttcgtg atcgctaaac agcgtacgcg cctgacccgg ccaggagtcg
     2701 gtgatcacca ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc
     2761 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagccc ggtagcgcca
     2821 ggcagtggcg tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga
     2881 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     2941 cccacggaac cgagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct
     3001 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     3061 tccaccacct gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc
     3121 gtggtcgcac cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc
     3181 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
     3241 agcgccgcgt acaccagata accgccggta gtatgcagca caccttttgg cttaccggta
     3301 gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc
     3361 tgatcgctcg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca
     3421 attttcccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
     3481 ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc cgcccggcac gcacaccttc
     3541 gtcggaagtg atcaccagtc gtgagttgga atcaataatg cgcccggcaa cggcttccgg
     3601 cgagaagccg ccgaaaatca ccgaatgcac cgcgctaatg cgggcgcagg ccagcatcgc
     3661 aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc
     3721 cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat
     3781 atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc
     3841 ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt
     3901 aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtaag gtttgatcca
     3961 gtcaagaatt tttccctgtt cgccccagaa ggtatcaggt acgttaatag attgttgata
     4021 cattgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt
     4081 gtgtttgtga atttggctca tgcttttgtt ctccttgtag aatgttaata atatgtgaca
     4141 taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca
     4201 aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga
     4261 ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta
     4321 actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt cacagtgtgg
     4381 ttatttgtta cacatagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg
     4441 aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4501 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4561 ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg
     4621 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4681 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4741 ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4801 aaaccctgcg taccggtgcg ccgaaaaatg ctgaagatgg tccgctaccg atggcgtgct
     4861 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     4921 tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact
     4981 gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5041 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5101 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5161 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5221 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5281 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5341 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5401 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5461 ctgccttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5521 ttgaagatca actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga
     5581 cggaagctga aatgaagcca attcaggacg atatccgtca tgcccagtgg cgctgggatc
     5641 tggcgatcgc ttcccacggc attcatatgc acgcaccgga agaaggttta cggatgctcg
     5701 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca
     5761 aaggcatcac ccatgaaatc cagatcccgg atatctcaac caaagagaaa gcccagcagg
     5821 ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga
     5881 tcccgcagtg ggaagaacag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg
     5941 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6001 tgctggggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctacccctgc
     6061 ggcgcaggca tctggtgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6121 tctggactgt cataaaccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa
     6181 cccgaataac aaattgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6241 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg tcggagagca
     6301 gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca
     6361 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6421 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6481 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6541 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6601 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6661 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcgaga agtgaacaag
     6721 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6781 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     6841 gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat
     6901 cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     6961 atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag
     7021 gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7081 ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac
     7141 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7201 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7261 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7321 gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg
     7381 tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt
     7441 ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga
     7501 tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7561 atttgcttgc ccagcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7621 gcctgctgac gcccgttcat cgcggactgg aaacgctgat gctggtgttg gcggtgctgc
     7681 tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc
     7741 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga
     7801 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcac tttgttcacc
     7861 ggatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc
     7921 tggcgctggg cgatgacggt aaagttcgtg cgctggtggc ggcattaggt ggcggtttct
     7981 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca
     8041 aatcgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8101 tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt
     8161 aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg
     8221 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8281 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtg cgcgctcctg
     8341 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8401 gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc ggtgtggggc
     8461 ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8521 gcctggcatt atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct
     8581 ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata
     8641 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8701 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8761 ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg cccgaatttg ctggcgctgg
     8821 gcgttacctg gctggagtgc attaacggcg gggatcatcc tcggttcctg gtgggcctac
     8881 tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaatgc ctctttatta
     8941 ccctggcttt ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt
     9001 tttcgccact ggtcgctgtt actggcgata gttactctga tgctgtcgct gctgggcacc
     9061 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc
     9121 gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9181 tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9241 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9301 tatccgatga tttacggtct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt
     9361 aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg tgattgtgct ggcgacgttt
     9421 gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt
     9481 gccgctggga tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9541 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9601 aaaggcaatt acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt
     9661 ggtgaactga tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg
     9721 tcaattcgct ggaacggcat ccatgactgg tatgtggtca tgggtgaaaa aaccggagcg
     9781 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
     9841 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa
     9901 aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac
     9961 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10021 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10081 gcgccatcag gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg
    10141 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10201 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10261 gagggcgaag cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt
    10321 ttgatggtat ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat
    10381 gcggaatatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa
    10441 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg
    10501 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10561 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgcggcgct ggcgacggtg
    10621 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10681 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10741 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10801 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    10861 tcggattaat catctggtga tgaagattat gttattgagc tgtataaaaa ataatcgaat
    10921 gaacatatgc caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct
    10981 ttccataaca aatggttatt cattaatcct gacataaact cgcgttatta tgcattaatg
    11041 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11101 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgcctggcag tatgtcacgt
    11161 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
    11221 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
    11281 ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc
    11341 gggtgatatc ttcatccatc tgattaaaat gattgttgtg ccgattgtga tctccacgct
    11401 ggtggtgggt atcgcgggtg tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac
    11461 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
    11521 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
    11581 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
    11641 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
    11701 cttcttctcg gtgctgtttg gtctggggct ttcctccctg cccgcgacgc atcgtgaacc
    11761 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
    11821 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
    11881 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
    11941 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggctcctgat
    12001 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact
    12061 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagcttcgt
    12121 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
    12181 tattttcatc gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt
    12241 gctgacgctg atggtgacct cgaaggggat tgctggcgtg ccgggcgtgt cgtttgtggt
    12301 gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg
    12361 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
    12421 ggtgctggtg attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga
    12481 gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccggggt
    12541 tatccacccg gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacc
    12601 ttaaccgttg gttaattttt caaactcttc acacccggta tcaaaccctt ccatacagct
    12661 caggttcaac cagtgcagcg ccttttgttt gttcttctcg ataaaacctt cttcaccgtt
    12721 taagaacatc attcccgccc agtactcgga ataaccggtt cgggaaattg cagagctgcg
    12781 tttgaaatac taggttgcct tgtcatcgtc ggccttaata cccacgccgt tggcgtaaat
    12841 caaaccaagc agcatttggg catcgacggc agagtcgttc tccagatctt ccgaggcttt
    12901 tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata
    12961 tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact
    13021 tttgcttgtg gatagtccag actgcccgga ttggtgattt tgatctgcgc caacagcgcg
    13081 caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa
    13141 tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgtcggtc cccggcctct
    13201 gctgccttta aatactggct gccaggctcg gagtcgttgg cgtgggcaaa gaatgtcaaa
    13261 aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca
    13321 gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggactg
    13381 tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcacg
    13441 tactgctcgg cggcgcgctg atcggcgatc ggctcgacgc gcacggcgca gtatttgtat
    13501 tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca ggcaccaatc
    13561 caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg
    13621 gtgataattt tgcctttacg cgagtgcacc caaaccaatg cctcatcttc aatacccaga
    13681 cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt
    13741 gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac
    13801 agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag
    13861 aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga
    13921 tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc cctgttcgcc cattttctcg
    13981 taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg
    14041 ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat ttgccagtcc
    14101 gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc
    14161 gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc
    14221 gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc ttcaaaggct
    14281 ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat aatgtacgcg
    14341 gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata gccggtatga
    14401 gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc cggatctttc
    14461 acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg
    14521 ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt accggtcagc
    14581 atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc
    14641 atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc
    14701 tcactggcgc tgacgccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt
    14761 ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt
    14821 tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt
    14881 gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt
    14941 ttcgccccgt tacgtttagc gttaattacg tgattcgcca tgattgggtg ggaatccgcc
    15001 gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc
    15061 attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga
    15121 gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag
    15181 ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac
    15241 ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc ccaggaaaca
    15301 ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg
    15361 atctgggtat cgttaatgaa gtcccagcca taataaccct tcagacacag ggtaccctgg
    15421 ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg
    15481 attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct
    15541 ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt tatgccacat
    15601 tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa
    15661 caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc
    15721 agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca
    15781 tcaactgaat gctgtggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg
    15841 caagcactgg caaccgggct tcggtggcct gtaagcggct ggttaagccg cgctgatagg
    15901 cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gcgttcagcc tgcatttctc
    15961 gttcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc accgcgttca
    16021 gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgagtg ccttcgagat
    16081 tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact
    16141 ggcgactggt ttttttgaat aaggtatcca ggtggatgga gtccagaccg aaaaacgctt
    16201 tgatatcaaa gctcgggtag aacagtgccc gcgcggaatc cacctgatct aatgacgcct
    16261 gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg
    16321 tcgccggaat gccggtctgg actcgtggta atgccaccgg tttgatctcc ggcatatcgc
    16381 tggcccccgc gccaatcaat gcacgcagag attctcgcgt ttcggtgatt tgccctttga
    16441 cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg aaaggcactt
    16501 gtgcttccag accgtgcgcc actttactct ggtgcgcttt caccgcgtaa tcaatcacat
    16561 cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct
    16621 gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat
    16681 gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc
    16741 cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccataccc agtgagggcg
    16801 catccatcgc ataagggctt aaaaagccgt tcgccgagac gcgttggcgg ttgagcatcc
    16861 ctaacgctgc gacctgtaat tgtgaaccgg catctaacaa atcggcctgg gactgcgctt
    16921 tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggatca
    16981 gcgcatccag ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc
    17041 tggcaagatg aatatcgtcg gccagtttga tttgttccgg tttgagctgt tgatgaggtg
    17101 cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc
    17161 acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt
    17221 ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg
    17281 ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct cttcaagcgt
    17341 tatttgagga gaacagctga gtgcggttgc cagaccggca gcgtattttt ccagggcgtc
    17401 ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg ccgagttcca
    17461 ggtggcatcc caggcgtgaa gaagatcgcg gccctgacga ataaccgttt gcgaacgttc
    17521 aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc gttggcacat
    17581 ctcttcacag gcattaaacg ccgcatgcag acctatacga atttgcagat aagtgcgcag
    17641 agccgtgact tcctgctggc gtgggatccg cattacttta cttagcatac ccagcgcacc
    17701 ggctagtttt tgcggcagag tgcgcgcttc actttcaggc cagacaaagg tgtaaatcac
    17761 cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt ccaccaggtc
    17821 gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacga ccatctgtgt
    17881 gccgatataa gaagagcgtt cagagctggt ggcaatccat gcgcccaaca ggaaaatcgg
    17941 tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca gtagcgtgaa
    18001 taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta
    18061 cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg tgtgaatgcc
    18121 ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca atgtttttac
    18181 cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg gcggtttggc
    18241 ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg tctggcagat
    18301 attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc gccagtcgct
    18361 ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg aggctaattt
    18421 ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt agcgattcag
    18481 cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc tttgagttcg
    18541 ccagttggca tcgtccgcga gcgccagcgc ctctctttca atccgcgttt cgggtagcgg
    18601 tgcgaggctg tctgtcaggt gactaatggc atcatcaagc cgatcattaa gcgcctgatg
    18661 catttgcgaa atggcacgac tgggaaacca cagcacgccg attaacgtca tcagcaaggt
    18721 tggatagagg ccaacaacga tacaccacag cgttaagcgc acgaccactt ccggatagtc
    18781 gagcatggcg gggaaggttt gcccgtaaat ggcgacaatg gcgacagcga aaaagaccag
    18841 tcccagccga tgggtgcgca tcaaaaacat gcagcccatc aggatcggcc cggcgatgat
    18901 caatcggatc aacggttcgc cgtatgacca tttgtagatc aaaaacaggc tgccgatctc
    18961 cagcaccgtg gcaaccacaa acaagatcgc cacaaatttg gtgtaaaacg cgttcgactg
    19021 aataccgtaa aacagcactg ccagcgataa cgccacaaaa gggatctcaa aagtcatcga
    19081 gatcagaatc accagcaggc agcctaccca gagttgcacg atctgcggca cgcggccggg
    19141 tcgccgctcg cttaactctt catgaaagaa cgccagcagc ctgaccaccg gtaatggcag
    19201 ggagttgagc gcgctcatta ttgcggctca agattagcga ctgccgaagc gccgatgcgg
    19261 aacatttccg ggtcaggttt atcgaccatg attttgaccg gaaaacgctg ggcaacgcgg
    19321 acccagttaa tagagcgaga aactttcggc aggccgccca gcaccaggcc gccgtcattc
    19381 ggtagcacac cgtagccaat cgaatccact ttaccctcga aggttttgcc gctgtcactc
    19441 atcaggcgaa tcgttgcggg tgtacctgag cgaatatttt tcagatcggt ttcgcggaag
    19501 ttggcgatca cgtaccagtg acgagtgtcg attagggtaa aaatagggcg catggcagaa
    19561 gcaaattgcc cgacggaggt tttgagggaa atgacccggc catcaaacgg cgcgcgaacg
    19621 gtcgccattt ccagatgcag tttggtcagg gcaatatccg cttcgaccgc cgcacgctgg
    19681 gcaactaata catccacgcc gctgacggcg ctggcggctg actgcgcctg taacaatacg
    19741 gcattaagat ccgcttctgc ggcgcgctgc gccgttcttg cacggtcaac atcttccgct
    19801 gagacaaaac cttctttcag taatggctcg gtgcggcgta atgtatctgt ggcctgtttc
    19861 gcggcggcac gtgctttttc taccgtggca ttaaccgagt cggcaccaaa ctgttgggcg
    19921 tcaacgctac gctgggtgag cataatttgc ttatccagcg ccgcgaggga ggcttcagct
    19981 ttcgccagat tggcttcgta cgggcgcggg tcaatgcgga acagcaaatc gccctgtttg
    20041 actgcctggt tgtcggtgac cgccagttct acaatgcggc cgctgacttc cggcaccaca
    20101 tcaatggtat ctgctgacgc gtaagcgtca ttagttgatg gcgcactgtc tacgcgccag
    20161 ataacgaaaa caagggcaac cagcgccaac gcaaccacta acagagcagg gaatttactg
    20221 cgaggagctt ttttcggcgt actttccatg agcgcgtctc aaatagatta gaaaaatgcc
    20281 agccagaaca gcatggcgta gacagcaaaa agggcggtat aaataattcc gacaaatgcc
    20341 agattgatat ttgcccgctg aataattcgc ctcgtgatta gcgtcaaaat aaggctggca
    20401 atggcgcaga aaaaccagct gggataataa gcgccgatca ccgggattgc cggagataat
    20461 gagcatcccg aaaccatgaa gacgggaatt atccaggctg tttttttgag ttgcatcgcc
    20521 atgcgagaga gaactgttgg catattaaat acatcctgtt tgctgcgaac aatatctgta
    20581 gtgaatatca atatccattg aaggcgtcag tatggctatt ttcttgatga ataaaaatag
    20641 ctttattcag ccaccgcatt cataacttat atattttatg tgttaattga ttgtatgtat
    20701 ttttataata aatgcttttg ggggcttaag cgcccccaaa agtgagtggc attaatttgg
    20761 ggttacgata ttcacccaca aatcgaaatt atccagacag gcaataattt cttccagttt
    20821 cgcgccattg ccaataattt tggctttctt cgcttttacc agatccgcca ttttggcttg
    20881 tcctgtcagc acggcgtgca gatcttcacg gctgatgtag aaagaggcgt cggcttgcgg
    20941 ttgcagtgtt ttacggtagt taagcacgct atcgttcagc gtcaggttga ggttatcgcc
    21001 gttgctcata ttgaagttca ggctgatatt tttacccgca gctttcgcgc tatcgaggcg
    21061 aacggacata aagtcgaaca gcatttcgac cgacatcccg cgaatggtgt ccggggaacc
    21121 ggtggtgccg tggctgaact tatgcacccc ttcgcgcagc tctttcgcgc cggtcaggta
    21181 gaaaccgcgc caggtggcgg actcggcctg atagcccagc tgttcaaagt tattcgcttg
    21241 cagattcttc gcgacctggt cacccgggtt ggcggcaatc acctgtttca gcagttctgc
    21301 cgaccagcgg taatcacctt gcttgttcgc ttcttgcgcc aggctgatga cacgggcaga
    21361 accgcccagc gcctgcacgt aacgtttacc catctccacc tgaccatacg gatgcaggtt
    21421 agccgggtta ccgtcgtaat agccaagata gaagttatac accgcgcggg cgttgtggct
    21481 gacagaaccg taatagccac ggctggccca gttattggca agtgcaggcg gcagtttaat
    21541 catgtcgccg atttcattca tggtgtagcc ctggttcgcc aggtgcaggg tctggtcgtg
    21601 aatgtacttg atagtatcgc gatatttacc aatataatca ttgatatgct tattgccccg
    21661 gaccggccag gtgtgcggca taaacaggac ttccgcgtcg ttaccccaca tatccagcgt
    21721 ttcgttcaga tactcggtcc acttgctggt gtcgcgggtt ttcgcgccgc gcagagtgta
    21781 gaagttgtgc agggtatgcg tggcgttctc ggcggtacac agcgctttca gggccggaat
    21841 atagaagtgc atttcggctg gtgcttcgct acctggggtc atcaggaagt caaactccag
    21901 gccgtcgata atcatcttct caccggtttt ggtgatggtt ttgttcggtg cgatgatgct
    21961 cgggtagccc gatgccagcg ttacgccaag accattgccg atgttaccct gagggttatg
    22021 cgccagcagc aggccgtagg agtaaagtgc acggtggctc ataatattac ccgccagtac
    22081 gttttcgctg atggcttcgt ccataaagcc agcaggggcg ataacctgca ctttaccgga
    22141 tttcacatca gcttcagaga taatgccttt cacgccgcca tagtggtcgg tatggctgtg
    22201 ggtataaata accgcaacaa tcggtttttg cgggcgattt tggaagtaaa ggtcaagggc
    22261 ggcttttgcg ctcggtggcg tcaccagcgg gtcgatgaca ataatcccgg tatcaccttc
    22321 gataaaggtg atgttggaga tatcctgacc gcgaacctgg tacattctgt cggtgacttt
    22381 aaacaggccg gaaataccgt ttaactgcga ctgacgccac aggctagggt taacggtttc
    22441 gggcgcgggc gcattgatat caaatttata atcttccccg cgataatatg gtttgccatt
    22501 agcatcgcgc agaatacctt catccagcag cggagcgata aatccacgct gagcgtcgtc
    22561 aaaatcctga cgatcgctaa acggtaatga tttggcgtaa ttctgattaa cctgttgggt
    22621 atattgagtg gcggctttcg cttcctcttt cgcgttagcg gtatttacca tcatgccgct
    22681 ggcggcagtt aacgcaataa caattctgct caaacggaat aaccgagagt tattcatata
    22741 aatctccatt tatcatttat gtcgtaaata tgtaatccat acaaaatacg tcatttaaag
    22801 ctggggttat cataattggg aatttgcgac tttaataagt ggaagtgtga gctgaacgat
    22861 ccattttatt aggcatttgt gctgaagaga gaaaattacg cgaatttatt atctctgtaa
    22921 ataatatgag gtgttttata tatttattat atatattgta cttcggcaaa tactgagcac
    22981 aatttatcaa tgcaaccggt gtttcttttt aaaaaacgta ccggagcaat gggcggtcat
    23041 gtcgctggca ccgcaggatt ctgcgttgct gtgccagcct tttattcatg gtcaatgagt
    23101 gaggtgattg ataaagacac caggtcgtca gagcgcactg attatccgca ttatgccagt
    23161 gaagttgcac tggcttttgg ttcacgagtg gttggcctcg aactggcaaa aatgattgtg
    23221 gatgcgtggc tgggcgcaca gtacgaaggc ggtcgtcatc aacaacgcgt ggaggcgatt
    23281 acggcaatag agcagcggag aaattgagat tcatccacta cttgcatgga tgagtaatga
    23341 ttaatgtgga tagagtttct ttttgaggtt ggctaatgaa acgctttccg ctatttcttt
    23401 tattcaccct gctcacgctg tccaccgttc cggcccaggc cgatattatc gacgacacca
    23461 ttggcaatat tcagcaggcg attaacgacg cttataaccc tgaccgtggg cgagactatg
    23521 aagattcgcg cgacgacggc tggcagcgtg aagtgagcga cgatcggcgc agacaatatg
    23581 acgaccgccg ccgccagttt gaagatcgcc gcaggcagct ggacgatcgc cagcgccagc
    23641 ttgatcagga gcgtcggcag ctagaagatg aagagcggag aatggaagat gagtatgggc
    23701 gatgaggttg ggtatggagc aggcatcgcc cctcacccca atggggagag ggagaaaacc
    23761 agcgcaatat tcaatatcga gcacaatcgg tcccctcgcc cctctgggga gagggttagg
    23821 gtgagggggc cagcgcgcac attccctctc atcacgccac cccaatctcc atcccatcaa
    23881 atcccgcctc aaaccccgac ggcagtgcgt tttccatcag ccacgcatca aactggtggc
    23941 tgatatgggt cagaatcacc cgtggcgagc ggataacctg attcagcgcc agcacggtgt
    24001 ttaaatcaca gtgactacgt ggcgcatcct cgcgcggcgg gtggctgcaa tcaatcacca
    24061 ttacctgcgg ctgattattg cgtaaaaatt tcagcgtttt ttccggcaag cctgcggtgt
    24121 cagacagcca cgccacccgg ctgtgtgccg tttccagcag ataaccgaag gtcagttttg
    24181 agtggttgag cggcaggggc gtgacctgta acccctgcaa atcaaacacc acaaacggtt
    24241 ccaccgtgtg gctgaaatca agcaggcccg gatgtttaaa cagatcgtcg cagccctgtt
    24301 catccggcgg gccgtaaacc gggatcggat cgcccacgcc ccagcgcagc gggaatagcc
    24361 cctgcacgtg atccatatgg tagtgcgtca gcaaaaactg ctggaacgat ccgggcggcc
    24421 agcgatcggc gagatcgtgc agcccggcgt cgatcagggt gattgcgtcg ttaaacttca
    24481 ctacgccgct gcatggctgg cggcgatact gcggtgagcg ccgcgctctg gcgcaggcta
    24541 cgcactcgca gccccatgcc ggaacgccct gtgcgccgcc ggtgccggtg agcgtgaggg
    24601 tcaggctcat gttacagcgc cttggtgaag cggaagtggc tctgctcgta gccttcacgc
    24661 aggtaaaaac ggtgcgcgtc gtggcgcttc acgttggtgg aaagttcggt catttcggcc
    24721 ccggcctggc gggcttcttc ttctgcccac gccagtaact tactgccgac gttcagaccg
    24781 cacgcctgcg gcatcaccac cagttcctga atttcgccga tccagttgac atgatgcaga
    24841 tgaaactgca aatgcaggcc gatcatgccg acaacttcgc catccagcag cgccagatgg
    24901 tagcgcatgc tcgggtagcg catgctcggg tcgcgcaggt tggcgttaaa gcccacgcga
    24961 aacgcgtggt ggtcaaactc cgcctgtttt agctcacaaa tcagcgcgta aaccgcgtcg
    25021 gtatcgtact gcgtggccgg gcgaagctca caagcaggca tggtgtttct ccttctgatg
    25081 aatcagcgtc agcagtgtgt cgaccgactg gcgcaggctg ccgtcattgt tgagcgtatg
    25141 gcaatcctgt ggagtatagc gggcggcgcg cgccagccgg gcgttaattt cactggtgtt
    25201 ttcacggcca cggtttttca ggcgctggcg caggatctcc ggcgaaacct gtaaacagac
    25261 gggcagcagc gccgattgat agcgcgcccg tgcctgcggc aaatgcgcgc gtgaaccgtt
    25321 gacgactacg tcgaatccag cgtgcagcca gaggtcaatc tcgatgccga cgccataata
    25381 cagaccgtta gcgtgccagc ttaaggccag caggttttgc cccgcgcggg taaaaaactc
    25441 ccgctcactc agggcgatat ggttttcact tccggcgctg gcgtcgcgcg tgatgtagcg
    25501 atgcgccacc agtaactgcg tttgttcccg caggcggagt tccgccagca ggctgtcttt
    25561 cccggagccg gacggcccca ttaaccaaat cagttttccc atcatcagaa caccctttta
    25621 ccctgacgcc agacgtggtc gatatgaatg tgattgccct ggcgatgcgc cagcaccagg
    25681 tcagcgcgtt taccctcgcc aatcaccccg cgatcctgaa gattcagcgc ctgcgccgga
    25741 tttttggtca ccagcctcac cgcctgcggt agcgtaaagc ggttgctctc gtcatcggcg
    25801 acgcggaacg ccgcatccag caggctggcg gggtagtagt cggaagagag gatatccagc
    25861 agaccaagct gcgccagttc actggccgcc acattgccgg agtgcgagcc gccacgcacg
    25921 atatttggcg cacccatcag cacgttcatg ccatgcttac gcgaggcttc cgccgcttcg
    25981 aacgtggtgg taaattcggc gatcacgctg ccaagttggt gggattcggc aacgtgggcg
    26041 tgcgtggcgt catcgtggct ggcgagcgca atttgtcgcg cgcggcacag ggcggcgatg
    26101 gattcgcgat tcggctgcga ccagcgtgcg gcgagcgcca attgctcttc ttcgtactgc
    26161 tgcatctgcg catcggtgag ggagtatttg ccctgataat attcgcgata cttctcgcgg
    26221 ttagcgaact ggcgctggcc cggtgagtgg tccattagcg acaccagcgt caccggttcg
    26281 cgctgaacca gtttttcaaa cagcggcagt gtggtgtgat gcggcagctc gcagcgcagg
    26341 tgcagacggt gctcggcgcg gttgacgccg cgtttctgcg tctcttcgat ggtgttgatc
    26401 atcttctcca gattctccag ctgatcgccg ccgtcgcgca cgtcgccaat cgccaccgcg
    26461 ttcagcacgg tggtgatgcc gcttgccacc atcagcgcgt cgtggctgct catcgccgaa
    26521 tgggcgggcc agtcgacttt cgggcgcggg gtgaagaatt tatcgaggtt gtcggtatgc
    26581 agctcaatca ggccgggtag tagccagcca ccttcgccgt ccatcgcttc tggcaggcgg
    26641 ctctggcttt cggcaaaggc gcggatttcg ccatcctgca cctccagcga accgtgcacc
    26701 acctcgtttt ccagcaccag cttaacgtta ttgataatca tgaagaggct cccattgggt
    26761 gcaggcggtc ggcgacgtca ttacgtacag cttcgtcatg gaagatgccg acgatggctg
    26821 cgccacgggc tttggcttcg tgaatcagtt ccaccaccgc ggcgctgttt ttggcgtcca
    26881 gcgaggcggt aggttcgtcg agcagcagaa tggggtagtc gacgataaag ccgcgggcga
    26941 tgttgacgcg ctgctgttcg ccgccggaga aggtcgatgg tgccaggtgc cacaggcgtt
    27001 ccggcacgtt caggcgggtg agaagacgcg cggctttagc ggcgcaggct tcacgcggaa
    27061 cgccggtatc gagcagcggc tgcatcacca cttccagtgc tgagatacgc gggatgacgc
    27121 gcagaaactg gctcacccag ccgactgtgg ttttgcggat ttccaccact ttgcgcgctg
    27181 gcgcggtgac caggtctacc cactcgtcac cgtgtttgat ctggatttga ccttcgtcgg
    27241 gtagatagtt ggcgtacagc gagcgcagca gggttgattt gccgctgccg gagtgaccgt
    27301 ggagcaccac gcattcgcct gcgttgacgg tgagcgaggc acgttgtaag acgggcaggc
    27361 gtacgccgtt ttgctgatgc aggatgaagg ttttactgac gttttgtacg ttaatcattt
    27421 ttcgcctcgt ggttcgggtg tcggatgcga cgctgacgcg tcttatccga cctacgggga
    27481 gcgcatttgt aggccggata aggcgtttac gccgcatccg gcaccggctc aattctgcaa
    27541 aaccgatgac accagcagtt gtgtatacgg atggtgcggg tcgtcgagca cgcggtcggt
    27601 taacccactc tccaccactt gcccctgctt catcaccagc aaacggtccg ccagcaggcg
    27661 ggcgacgcct aaatcgtggg tgacaatcac taccgcgagg ttcagctcca ccaccaggcc
    27721 gcgcagcagg tcgagcaggc gtgcctgtac cgacacatcc agtccgccgg tcggctcgtc
    27781 cataaacacc agcttcggat gcgtcaccag gttgcgggcg atctgcaaac gttgctgcat
    27841 gccgccggaa aaggtggtcg gcaggtcgtc gatccggttg gcgggaattt ccacctcttc
    27901 cagccacttc tgcgcggtgg cacgaatatc gccgtaatga cgtgccccgg tcgccatcag
    27961 ccgctcgccg atattgccgc ctgccgacac ctggcggcgc aggccgtcga gcggatgctg
    28021 atgcactacg ccccattcgg tgcgcagcag gcgacggcgg tcggcctcgc tcattgcgta
    28081 cagcgaacgg ctctcatagt gaatctcccc ctgctgcggc gtcaggcgcg cggagattga
    28141 tttcagcagc gtggtctttc cggagccgga ctccccgaca atgcccagca cttcccccgg
    28201 ccataaatca aaagagacat cgctaaagcc tttgcccggc gcgtaaaggt gggtcaggtt
    28261 attgaccgaa agtaacggtt gattcattgg ttttttgcct cgctctgttg gcggcaataa
    28321 tcggtatcgg agcagacaaa catgcggttt ccggcgtcat ccagcaccac ttcatcaaga
    28381 tagctgtggg tcgatccgca gatggcgcat ggctcatccc actgctgaac ggtgaacggg
    28441 tgatcgtcga aatcgagact ttccacgcgg gtaaacggcg gcaccgcata gatgcgcttc
    28501 tcgcgccccg cgccgaacag ttgcagagcg ggcatcatgt ccatttttgg gttatcgaat
    28561 ttcgggatcg gcgacgggtc catcacgtag cgcccgttca ccttcaccgg ataggcgtag
    28621 gtagtggcga tatgaccgaa gcgggcgata tcttcataca gtttcacctg catcacgccg
    28681 tactcttcca gcgcgtgcat ggtgcgggtt tccgtttcgc gcggctcgat aaagcgcagc
    28741 ggttcgggga tcggcacctg gaagataatg atctgatctt cggtcagcgg cgtttcgggg
    28801 atgcgatggc gcgtctggat aagcgtcgca tcgtccgtac gttcagtggt gtttaccccg
    28861 gtcacgcgct taaagaaatt gcgaatcgac acggcgttgg tggtgtcgtc tgcgccctgg
    28921 tcaatcacct tcagcacgtc gctttcgcca atcacgctgg cggtgagctg aatgccgccg
    28981 gttccccagc cgtagggcat cggcatctcg cgcccgccaa acggcacctg ataaccgggg
    29041 atcgccaccg cttttaagat ggcgcggcgg atcatgcgtt tggtctgctc gtcgaggtag
    29101 gcaaagttgt agccgctcag attagccatg gttctgctcc tgttgcagac gtttgagtag
    29161 ctccagttcg gcctggaaat cgacgtagtg ggggagtttg aggtgcgaga caaagcctgc
    29221 ggcttcgacg ttgtcggcat gtgccagcac gaactcttca tcctgcgccg ggcctgtcgc
    29281 gtgctcgccg tactccggag cctgcaacgc acggtcaacc agcgccatcg ctttgcgctc
    29341 gctcatgccg aacaccagcc cgtagccgcg cgtgaagtgc ggcggctcat ccggcgggtc
    29401 aataaaaccg ttgaccattt cacactcggt catcagcagt tcgccgacgt tcaccgcaaa
    29461 tcccagctct tccggcataa tcgacacgtc gatgtaacca ctgcggattt cgcccgcgaa
    29521 cgggtgattg cgtccgtaac cgcgctgggt agagtaggcc agcgccagca aatagccttc
    29581 gtcgccgcgc atcaactgct gcaaacggga agaacgtgaa caggggtaaa ctggcggagt
    29641 gcgggtgatg tcatccggct gtgcgccgct atcctcttca aacttcgcca gcccctgacg
    29701 cgccagcagg ctgaaaacgt gcggcgacgg ggattgttcg ctgtctgcgg tggtcagcgt
    29761 cggcgcttcg ccgtttgcca gcagggtgaa atcgagcagg cgatgggtgt agtcgtaggt
    29821 tgggccaagc aactgaccgc cgggaatatc tttataaacc gctgaaatac gccgttcgag
    29881 acgcatctcc gtggtgtcga gcggctcgct taccgccagc ttcgccagcg tggtgcggta
    29941 ggcgcgtagc aggaaaatcg cttcgacgtt atcgccgctg gcctgtttca gcgccagcgc
    30001 cgccagttcg cggtcggcaa tgccgccttc ggtcatcacg cgatctactg caagattaag
    30061 ctgctgttca atctgggcga cgctcagttc aggcaaatcg gtattgcccc ggcgtcggct
    30121 ctcttgcagg gcgtgggcgg cgtcgatctc cttctcgccc cctttcacgg caacgtacat
    30181 cagcacacct ccacatgagt ggttcgcggg atagccagca gccgctcgcc gcaggtcagg
    30241 atcaggtcga tgccgagcgg gaacgggtgc gggcgctcgg tgagttcgtg cagaatgcac
    30301 tccggcagct gcggagcgat cattcgttct tcggcaatac ccgcgccagt aaggcgcagc
    30361 atgcgcccgc cgctcaggct gacgacctgt aaaatcagcg tcgcgcccgc ttccggcgca
    30421 acagcggtgc cgctggaaag agcgttgagc tgttcgctgg aaatcgcctc atccgttacc
    30481 gcgaaggtcg ccagttccga ctgattgacc agcggcgcgt tggtatgaaa acgcaggctc
    30541 tggctgacga tatcgttact taatggcgcg gaaagccaca tcggcgtgtc gttatcagtc
    30601 agcgtcagca gcacgctggt ggtggcgata ttcagcggct gccagccgcg tttgagctga
    30661 tgcagggcga caatcacgcc cggctcgctc atggccttta acaggcgacg aaaactgtgc
    30721 tgggcatcct gcacaggaag cataaaagcg gtttccaggg tcatgcgttg tctccgcgaa
    30781 ccatcgtaaa gaagtcgacc cggctggcgt tcacttcggc ctggcgtgcg gcaatgcgtg
    30841 ccatacggtc agcgtccagt ggggcaataa gggtttctga taagttttga aaatggcgag
    30901 attgctgcat caacgcgtca atcagcgcgc agcgttcggc gtgctgtttg tcgcgcccca
    30961 gcacccagct gtagccgagg gggccgtcag tcaggcgtac ggcggcgcgg gtcagcgtgg
    31021 cgtcgccggc aaaaaagcgt tcgccggtac cgcccattcg cggctgaatc tgtaccaggc
    31081 cggtttcggc ggtgcggatc acttcatagt tggcggtgat gttcagcgcg ttcaggcggg
    31141 ctgccagttc agccggttgg ctgtgcgcca gcatggacat ccagtgctgg cggggcgcga
    31201 tatctgcgtg cattcagtgc tccatagtga attcaatcat gtcggcgcgc gtcaggctga
    31261 cggagtactc cgccgggctg ctttcaccgt cacggtggtt aagggtgcgc acgcacagca
    31321 gcggcgacat attcgggatt tcaagacgct ggcactcttt ggcctgggcg cggcgggcgc
    31381 tgatccgcgt ctggctgcgg cgcagcgcaa tcccggtttg ctcgcgcaga aaatcgtgca
    31441 gcgagccgct gtcgaagcgt tgcagcgtcg gccaaagggt gaggtccgca aagtagtggt
    31501 cgattaaaca gagcgcgatg ccgttgaccc ggcgcagggt gcgcaggtgg atgacgttct
    31561 ccccctcggt tatccccagc gcgtcagcga cgtgaccaga tgccgggcgc agcaccgaca
    31621 gcagtttttc gctggtggga tggctgccct gatccagcag gttctggcta aaacgcgcct
    31681 gggcgttgag cgggtaatcg aacgggcgca tcagcaccag cacgccgacg ccctgacggc
    31741 gctgtaccca gcctttttcc accagttggt cgatggcgcg gcgcagggtg tggcgattta
    31801 cctcaaagcg cgccgccagc tgctgctcgg cgggaagata gtcgccgcag cgatagtgtt
    31861 gacgaagctc ctgctcaagt tttgcggcta tctcctgata gcgtgttggg tagctggtcg
    31921 gatgtgtaga caagtgcata gatatcaatg cctcgcttat cagataaagt gcttacgcaa
    31981 ccgttgagag aggaaatcca gcaggctgac cgtgacgatg ataagcacca tcagggcgca
    32041 ggtttgttgg aactgaaaac cgcgaatcgc ttcccacagg gtgacgccga tcccgcctgc
    32101 accgaccatg ccgacgacgg tcgccgagcg gacgttggat tcgaagcgat agagggagta
    32161 ggagatcagc agcggcatta cctgtggcag cacgccgtaa aggatctctt ccagcttgtt
    32221 ggcgccggtg gcgcgaatgc cttctaccgg gccgggttcg atcgcttcca ccgcttcgga
    32281 aagcagcttg gagagcacgc cggtggtgtg gataaatagc gccagcacgc cagcgaacgg
    32341 gccgagaccg acggcgacca cgaacagcat ggcgaagacc atttcgttaa tggcgcggca
    32401 ggcatccatc aggcggcgaa cgggctggta aacccaccac ggcaccaggt tttcggcgct
    32461 catcagaccg aaggggatgg agagaaccac tgccagcgcg gtgccccaga cggcgatttg
    32521 cagcgtgacg gccatttcgg tgaggtaatc ctgccactgg ctgaaatcgg gcgggaagaa
    32581 gtcggcaacg aaggttgcca tgttgccgcc gtctttaatc agcgtaagcg gggccatttc
    32641 cgcgccctgc cacgagacga ccagcacggc gagaacaacg gcccagctca gaagcgagaa
    32701 ccagctacgc ttgggtgggg cgatggtgat ggtttgcatg tttggctccg tttaatgttt
    32761 tctccctctc cctgtgggag agggccggga tgagggcatc agcgtgcgca tttccctctc
    32821 accctaaccc tctccctcaa gggagagggg accgatcgag cacattactg caccgcttta
    32881 ctcaccgaac tcatcgcgct taacgcgttg ttcaggcggt ccaagtcatc cagctgcgcc
    32941 tgaatctcgg tggttttcgc cagcttgtcc tgctcattca gtcctttatt gctttttacg
    33001 ccctgcatct ctttaaacag tgcgagctgg cgaatcggca ccagttgcag gtcgctggag
    33061 gcgcggaacg gggcccagcc caggcgttcc agcaccgctt tctcttccgg cgtttttccg
    33121 tagttcataa agaagtcgta gatcttgtct ttggtggttt cggagagatt cttgcgccag
    33181 acgatcggat cgcctgggat cagcggcgac ttccagatca ccttcagttc tttcagcttc
    33241 tctggcgcgg aggttttcag cttgtcgagg ttttcggtgt tgttggtggc aacatccacc
    33301 tgcttgttgg cgacggccag cgcgttggtt tcatgcccag cgttgacggt gcgcttaaag
    33361 tcgctggcgg agatattgtt tttggcaaag acgtagtaac cggggacgag gaagccagag
    33421 gtggagttag gatcgccatt gccgaaggtg agatctttcc gcttcgccag cagatcgttc
    33481 aggttgttga tcggactatc tttgttgacg atcaacacgc tccagtaacc tggcgatcca
    33541 tccgccgcga ccgtctgggc gaagacctgg ccgttggcgc gatccaccgc ttccattgcc
    33601 gacaggttgc cgtaccaggc gatatccact ttattgaagc gcattccctg gataatgcct
    33661 gcgtagtctg gggcaaagaa ggcgttcacc ttcacgccca gcttcttctc catatcctgt
    33721 aagaatggcg tccattgcgg tttcaggttt tgctgtgatt ccgttgaaat aatgccgaaa
    33781 ttcagcgcct tttcctgctc ttcggcgtgt gccgggctta acagggtgct gaggctgaac
    33841 atgctggtga aggccagcga ggcaattatc ttagcgttca ttcgttttcc tcaatgatgg
    33901 ggatgtcagg cagctttcgc gttctcttcg atgcgattaa tgctgcggta gagatggtca
    33961 aaacgttcgt tatcaaactg ttggctgctg ccgtcgtaga aaacgtgccc ctggcgcagg
    34021 gcgacgatgc gttcgcagta gcgcagggcg taatccacct gatgcagcgt gacgactacg
    34081 gtgatgccgt cgttctgatt gatgtcgcgc agggtgtcca tcacgatgcg ggcggattcc
    34141 gggtccagcg aggcgatggg ttcatcggcc agaatcacct tcgcctgctg catcagcgcg
    34201 cgggcaatcg ccacacgctg ctgctgtccg ccggagaggg tggaaacgcg ttgatgggca
    34261 aaatgcacca tgccaacgcg ggtcagcgcc tgtaacgcgc gttgtttctg ctcgcgggtg
    34321 aaccagctaa aacaggtgcg ccagaacggc gtgctgccga gcgcgccaat cagcacgttc
    34381 tccagtacgc tcaggcgatt caccaggttg aattgttgga agatgtagcc ggtatgggcg
    34441 cggcttttgc ggatatcgcg cgccagacgg ccttcgcgct ggactgtgcg gcccagcagc
    34501 tcgatatggc tgccggcgga tttatcgccg gtaatcaaac cgcttaagtg acgtaaaagt
    34561 gtggatttgc cggaacccga cggcccaagc agagccacca tttcactgtg atgaatgttc
    34621 agatcaaccg catgcagcgc ttgatgctga ttgaaggttt tggcgagctt ctcgacacgg
    34681 ataatcgttt gcatgatgca gcctccctaa aaaagtggct ccatcgtggc ggattattgt
    34741 gacgatttgg ttaattaaaa gtgactaaca gatgaagagt taacgggaat tcgatgacag
    34801 tgcgggaggg cgggcctccc ggcgggttat tgcgttggtt gttgtttgac gacattaatc
    34861 atccacggta cgccaaattt atcggtgact ttgccgaagc catgcgccca gaaagtttcc
    34921 tgccaggcca tttcaatttt tccgttagcg gcaaggttat caaaccagcg ttttccttct
    34981 tcgacctgtt gcgaatcgag caccagcgta aagccggagt agctggcttt tcctgacggt
    35041 atagcatcgc tcatcatgat gtcgcttccg gcaatgcgca cgttggcgtg ggcgatggcg
    35101 gtatcgggaa attgcattcc ggaagggcag ttctcggcgc tgtcctgcgc tgattttggc
    35161 atttcgccga agctgatttt atagagcagt tccgcgccca acgtacgttg ataataggca
    35221 atcgcgtcgg aacagttacc ggcaaaagag aggtagggac ttaacggcat gatggtgacc
    35281 tcagttaaga gaagccagtt aagtgtagtt cgaaatttat acagatgaga gggaagcctg
    35341 ataaggcgcg gtcagcgtcg catcaggcag tcggcatcgt tgccggatgt ggcgcgagcg
    35401 ccttatccgg cttaccgatc acaatacaat cagttctttt tcacaaactc agatttcagt
    35461 ttcatcggac caaaaccgtc gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg
    35521 atgtttttca ctttggtgcc aattttcagc atcgaagagc tacctttcac cttcagatct
    35581 ttaatgatgg taacgctgtc gccgtcagcc agcagattgc cgttagcatc tttaacgatc
    35641 agctcgtcgc tttcctgtgc aggttctgtg tcgttccatt cgtaggcaca ttccgggcag
    35701 atgtacatgc cgttatcttc gtaagtgtat tcggagttgc attttgggca gtgtggtaat
    35761 gacatgtgga tttcctcaaa agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg
    35821 aaaaaggccg caattataca caaaatccct gagtttgtcg ggggtactgc tgattttaga
    35881 cgacgatcgt tttcatttga aaggaaacgc aaaaagaaaa taatcttttt gcctctattt
    35941 attatttaag ataatgttaa tgctctacct gttctttttt tattcacatg atatttttat
    36001 ctttctgacg ttttaaacgc atcagaagac gttatttatt ctgataaaaa acaacacact
    36061 aaatataaaa t
//
LOCUS       DADLTD010000016        34523 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLTD010000016 DADLTD010000000
VERSION     DADLTD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.016"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            235..718
                     /locus_tag="JN701_000625"
                     /pseudo
     CDS             235..718
                     /locus_tag="JN701_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated peptidase"
     gene            821..944
                     /locus_tag="JN701_000626"
                     /pseudo
     CDS             821..944
                     /locus_tag="JN701_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 domain-containing protein"
     gene            1177..1323
                     /locus_tag="JN701_000627"
     CDS             1177..1323
                     /locus_tag="JN701_000627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363161.1"
                     /translation="MEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHALT
                     VIVG"
     gene            complement(1390..2847)
                     /gene="nuoN"
                     /locus_tag="JN701_000628"
     CDS             complement(1390..2847)
                     /gene="nuoN"
                     /locus_tag="JN701_000628"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY7363162.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2854..4383)
                     /gene="nuoM"
                     /locus_tag="JN701_000629"
     CDS             complement(2854..4383)
                     /gene="nuoM"
                     /locus_tag="JN701_000629"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY7363163.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4547..6388)
                     /gene="nuoL"
                     /locus_tag="JN701_000630"
     CDS             complement(4547..6388)
                     /gene="nuoL"
                     /locus_tag="JN701_000630"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY7363164.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6385..6687)
                     /gene="nuoK"
                     /locus_tag="JN701_000631"
     CDS             complement(6385..6687)
                     /gene="nuoK"
                     /locus_tag="JN701_000631"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY7363165.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6684..7238)
                     /gene="nuoJ"
                     /locus_tag="JN701_000632"
     CDS             complement(6684..7238)
                     /gene="nuoJ"
                     /locus_tag="JN701_000632"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY7363166.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7250..7792)
                     /gene="nuoI"
                     /locus_tag="JN701_000633"
     CDS             complement(7250..7792)
                     /gene="nuoI"
                     /locus_tag="JN701_000633"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY7363167.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7807..8784)
                     /gene="nuoH"
                     /locus_tag="JN701_000634"
     CDS             complement(7807..8784)
                     /gene="nuoH"
                     /locus_tag="JN701_000634"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY7363168.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8781..11507)
                     /gene="nuoG"
                     /locus_tag="JN701_000635"
     CDS             complement(8781..11507)
                     /gene="nuoG"
                     /locus_tag="JN701_000635"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY7363169.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11560..12897)
                     /gene="nuoF"
                     /locus_tag="JN701_000636"
     CDS             complement(11560..12897)
                     /gene="nuoF"
                     /locus_tag="JN701_000636"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY7363170.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQSVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12894..13394)
                     /gene="nuoE"
                     /locus_tag="JN701_000637"
     CDS             complement(12894..13394)
                     /gene="nuoE"
                     /locus_tag="JN701_000637"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY7363171.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13397..15199)
                     /gene="nuoC"
                     /locus_tag="JN701_000638"
     CDS             complement(13397..15199)
                     /gene="nuoC"
                     /locus_tag="JN701_000638"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY7363172.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15293..15955)
                     /locus_tag="JN701_000639"
     CDS             complement(15293..15955)
                     /locus_tag="JN701_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY7363173.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15971..16414)
                     /gene="nuoA"
                     /locus_tag="JN701_000640"
     CDS             complement(15971..16414)
                     /gene="nuoA"
                     /locus_tag="JN701_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY7363174.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17045..17983)
                     /gene="lrhA"
                     /locus_tag="JN701_000641"
     CDS             complement(17045..17983)
                     /gene="lrhA"
                     /locus_tag="JN701_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY7363175.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSSDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18881..20098
                     /locus_tag="JN701_000642"
     CDS             18881..20098
                     /locus_tag="JN701_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY7363176.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20182..20781
                     /gene="yfbR"
                     /locus_tag="JN701_000643"
     CDS             20182..20781
                     /gene="yfbR"
                     /locus_tag="JN701_000643"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7363177.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20840..22672)
                     /locus_tag="JN701_000644"
     CDS             complement(20840..22672)
                     /locus_tag="JN701_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY7363178.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22759..23409)
                     /gene="hxpA"
                     /locus_tag="JN701_000645"
     CDS             complement(22759..23409)
                     /gene="hxpA"
                     /locus_tag="JN701_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY7363179.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDTPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(23420..23914)
                     /locus_tag="JN701_000646"
     CDS             complement(23420..23914)
                     /locus_tag="JN701_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY7363180.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(23998..24453)
                     /locus_tag="JN701_000647"
     CDS             complement(23998..24453)
                     /locus_tag="JN701_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY7363181.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24791..25993
                     /gene="ackA"
                     /locus_tag="JN701_000648"
     CDS             24791..25993
                     /gene="ackA"
                     /locus_tag="JN701_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY7363182.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26068..28212
                     /gene="pta"
                     /locus_tag="JN701_000649"
     CDS             26068..28212
                     /gene="pta"
                     /locus_tag="JN701_000649"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7363183.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28402..29922
                     /gene="yfcC"
                     /locus_tag="JN701_000650"
     CDS             28402..29922
                     /gene="yfcC"
                     /locus_tag="JN701_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY7363184.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQITVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29955..30497)
                     /gene="yfcD"
                     /locus_tag="JN701_000651"
     CDS             complement(29955..30497)
                     /gene="yfcD"
                     /locus_tag="JN701_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY7363185.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30555..31106)
                     /gene="yfcE"
                     /locus_tag="JN701_000652"
     CDS             complement(30555..31106)
                     /gene="yfcE"
                     /locus_tag="JN701_000652"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY7363186.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31162..31794)
                     /gene="yfcF"
                     /locus_tag="JN701_000653"
     CDS             complement(31162..31794)
                     /gene="yfcF"
                     /locus_tag="JN701_000653"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY7363187.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31930..32577
                     /gene="yfcG"
                     /locus_tag="JN701_000654"
     CDS             31930..32577
                     /gene="yfcG"
                     /locus_tag="JN701_000654"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY7363188.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32634..32996
                     /gene="folX"
                     /locus_tag="JN701_000655"
     CDS             32634..32996
                     /gene="folX"
                     /locus_tag="JN701_000655"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY7363189.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33017..33910
                     /locus_tag="JN701_000656"
     CDS             33017..33910
                     /locus_tag="JN701_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY7363190.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33958..>34523)
                     /locus_tag="JN701_000657"
     CDS             complement(33958..>34523)
                     /locus_tag="JN701_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7363191.1"
                     /translation="GYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSA
                     FPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKA
                     LFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQE
                     MLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8659 a         9391 c         8637 g         7836 t
ORIGIN      
        1 tgtatagatc taacaaccgt aataaaacaa atactcataa ctcattataa ataatgataa
       61 aataacgtct tttattgata atttatatat gggaaaaatt ccttttgtta tattgtttct
      121 gatttcactc cctctgatta tttttaccaa ccctgggtca ctgcattacc accaacgccc
      181 cgacacgtca gtcctgagca attagaaaaa acagttcgtt atcttacaca aactgtgcat
      241 ccacgtaatg ccgacaatat tgataatctg aataggtccg tagagtatat aaaagagttc
      301 tttgtcagta gcggtgccag agttaccgcg cagggcgtcc ccgattggct attacgactc
      361 tgcgcctggt agccaggatt acctttaccc gcaatgtcct ggctttatcc cgatcggggg
      421 gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag gtaaaaacgg
      481 cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg tttattcccg
      541 gtattgattt ctctgaccac ctgaattatt ggcaacacga tattcccgcc gtaatgatta
      601 ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgataca gcagacagat
      661 tgaattatca gaaaatggct cagatggtgt tataaccttg ttacacaaca gtaaataata
      721 aaaatttctc gaggcgggtc cagaaacagc gacatgtaca ttttattttc cctcgtctgt
      781 tttaagatgc gaaacataaa attcaatgga ggaaaaataa atgggaatga ttggttattt
      841 tgcagaaatt gactcagaaa agattaacca atctgcttga gtctactgaa aaacctttga
      901 tggataatat acatgacacg ctttcaggat tacgccgact ggatattgat aaacgctggg
      961 attttttaca ctttgggctg acaggcaccc ctgcttttga tcccgcgaag aatgatccgc
     1021 tgagtcgtgc cgtgcttggt gaacacagcc tcgaagatgg catagatggc ttcctgggat
     1081 tgacctggaa tcaggaatta gccgcgacaa tcgaccgact ggaatcactg gatcgaagta
     1141 agctgcgtaa acagttttcc attaagcgac tatgaaatgg agatttatcc gggagtgacc
     1201 ttttcagaag agttagaggg gcaactcttt gcctcgatca tgcttgatat ggaaaagctg
     1261 attagcgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt tattgttggg
     1321 tgatggtgtc agagcgccaa aaaaaaccgc cgaacatgtc ggcggttttc tgacttacaa
     1381 actaacagat tacatcagcg gcattgctaa ccgcacaata ctaatcagcg attgtggcca
     1441 tacacccagc accagtacca acagtgcaga aatcagcacc acgataccgc ccgcgctgta
     1501 ctgccagttt gacggtgcat cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac
     1561 cgccacgcgc aggtagtagt agaggccgat tgccgaaccg acaaccacgg cacccaccag
     1621 ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccga taaagcccag
     1681 cgtcatcggg ataccggcca gagacagcat catcaccgtc atcactgccg cgaggatcgg
     1741 acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc cacgatacgg
     1801 gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat aaccggccag
     1861 gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca gcgccaccag
     1921 cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatgt tggtctggct
     1981 caacgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca gtactacgcg
     2041 aatcgcttcg ctgtcaccca ccggtgcata gaggaacaga cgcatcacca caccgaagat
     2101 agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac
     2161 gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc caacaatcat
     2221 catgccgaaa cctgccagca acagcggctc gttgagcata ccgtcgccga ggtttttacc
     2281 cagcgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac caaacagcag
     2341 gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca gcgaacgttt
     2401 ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt caataccgag
     2461 gaacagagac gccagatggt ttgcattcgc cagcaggatc ccgcccagcg cggcaattaa
     2521 caccaacagg tagaactcat ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa
     2581 agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc
     2641 aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccaacaaacc agagcgaaac
     2701 cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca
     2761 cgcaatggag agcatcacaa ccaccaccgt taagccgacg atcagcaacg gtagcagtgc
     2821 gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt
     2881 aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg
     2941 atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag
     3001 cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga agtaagcgcg
     3061 atgcaacatc gccagcgaat aaacagatgc aaagaccagc ccaaaggtag agataacggt
     3121 aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga cgaagttacc
     3181 ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacaatgctg gcagccattt
     3241 catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag
     3301 ctgaccacaa agaataaaca gacccgccgc cgacaaaccg tgcgcaatca tctggattac
     3361 cgcgccctgg taggccaact ggctgccggt gtagatagca atcagcacga agcccatgtg
     3421 ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg ccatccacgc
     3481 accgtagaag atgccgataa cacccagcca catagcaatt ggcgcgaact ctgccgacgc
     3541 gttcgggaac agcggcaggg agaaacgcag caaaccgtaa gcggcagttt tcagcaagat
     3601 ccccgcgaga tcaacggaac cggcggtcgg agcctgggag tgcgcatccg gcagccagcc
     3661 atgcagcgga accaccggca ttttgactgc gaaggcgatg aagaagccca gcattaacag
     3721 gtattccaca ccactggaca ttggcgtatt cagcagctct tcatagttga aggtccagac
     3781 gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg gcaatcaaca tcaccaggcc
     3841 actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga
     3901 ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa
     3961 gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca
     4021 catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg aacagagtac
     4081 cgccagcaca ccgagcagac cggtcagcac gaccatcagc agcgacagac cgtcaatggc
     4141 gagatgaata gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg
     4201 cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag
     4261 cgccagcgtc aatcccatgg tgatcagcgc gatccagcgt ggcaccttga cgtcaaagtg
     4321 ttcggtctgc cagcacagga agccgccaat aaagggaatt aatattagcc agggtagtaa
     4381 catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca
     4441 cgacagaaaa ttctcttcgg actgggggta gtgtcggatg cgccgcgaag gcagcttatc
     4501 cgacctacgt tcttatatgc ccaggggcaa tcccacaatc cttaactcaa cgcagtacca
     4561 tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac cagcgcagat
     4621 aaccgttctc gcttaacagc aggcctttac ctgcaaagcg ggaaagaacc gccggaatgt
     4681 tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccaga aacggcttga
     4741 cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag gtgcccagca
     4801 gacggcccgg cgcactgttg gcaatggagg tcaccagagt acgtttaccc tgccacagcc
     4861 aggctgccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc agggtcaaca
     4921 tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgct ctgcagcggc ggtacaatca
     4981 gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg tgagttaccc
     5041 ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa atcatacgga
     5101 aggtgtagag cgaggtcata aacgcaccga ccagacctgc caccatcaga ttgatatgac
     5161 cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca gtgaccagcg
     5221 gtagtgccga cagtgctgcg ccgcccacca ggaagcagaa ataaaccagc ggaatagatt
     5281 tacgcagacc gcccatcttg aagatgttct gttcgtgatg gcaggccaga atgacggaac
     5341 cggatgccag gaacagcagc gctttaaaga acgcgtgagt catcaagtgg aaaatcgccg
     5401 catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc atggtagagt
     5461 aagcgagaac acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg
     5521 taaccgcccc gacaatgccc accagatgca gaatttccgg cgtcatcagg aacaggccgt
     5581 gggtacgggc gatcaggtag acacccgcgg ttaccatggt tgcggcgtgg atcagcgcgg
     5641 agacaggcgt cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc aactgcgcag
     5701 atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg ttattgccgt
     5761 cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg cccagttcgt
     5821 tgtaaagaat gaacagcgcg aaagcgagga acacgtcacc cacacgggtc acgacgaacg
     5881 ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaacccgatc agcagatagg
     5941 agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttatcg gcgagaacca
     6001 gaactaccat gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag tagccctctt
     6061 caccgcgcat gtaccaggag gcgtacatgt gaataaggaa acccacacca gtgaccaccg
     6121 agagcatggt cagcgacagg ccgtccagca ccaggttaaa accgatgtta aagtcgccta
     6181 ccgacatcca cgtccacagc ggctggctgt atgcctgctc gccgttagcg aagaaatcaa
     6241 cgccgataaa ggcggttacc agcgccgcca ggcccacaga gccaacgccg acgatcgccg
     6301 aaacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca atcaatggca
     6361 aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat cgatgttcag
     6421 gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg cttctgccgc
     6481 cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc
     6541 ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa tcagcataaa
     6601 cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga ttgccgagag
     6661 gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg
     6721 tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccgacgtg gaaggccaca
     6781 accagacccg cgagcagcag catagaagcc agttccaccg ccagtacgta aggcccgaac
     6841 agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg atcattaaca
     6901 ccaaggatgg cgtaaacaat caccaccagc atgatggccg acaaaattgc cggaccaatc
     6961 cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc
     7021 atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat aatttccagc
     7081 gcaccggcga agtaagcgcc cagtgagaag aacaccccgg aaatcgccag cagcgaaata
     7141 atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa ggtcgcaagt
     7201 atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct tacggtaaca
     7261 ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct ttgccgtcga
     7321 ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga ccggagatca
     7381 gcagatcctc tttttcgtaa accagatcct ggcgcttgta ttcccccatt tcgaaatccg
     7441 gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag aaaatgcagc
     7501 gtgagaagtt gatgcggaaa aattccggat accagcgacc gtctttggtt tctgcttttt
     7561 gcagcgagat acagccgacc gggcaggcta ccgcgcagag gttacaggct acgcaacgct
     7621 cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg ataacggggc ggcagataga
     7681 ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc
     7741 agatactacg aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc atggtcttat
     7801 tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt tgatcagcgt
     7861 cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg gacgcggtaa
     7921 cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcaacg cgaaccagat
     7981 gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca tcaatgcaga
     8041 gatggtcaca atcccgatgt actcacccac gaagaacaga ccgaacttca taccggaata
     8101 ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg
     8161 gtgacatacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt gcgggataac
     8221 gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac cggcctgcgc
     8281 caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc
     8341 acgcatcgca cccagcaacg agtatttgtt gttacttgac cagcccgcaa acagcaccgc
     8401 gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac
     8461 cacccaaccc ggactgactg gcacaatcgc aaaggccagc agcagcgagg taaaggcaat
     8521 catcggtgcc agggtaaaga tgacgcgatc cgagaatttc gggatccagt cttctttaaa
     8581 gaacattttg atcatgtccg caaccagctg gagcgaaccg ccccagccaa cacggttagg
     8641 tccataacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca tgaatgcccc
     8701 acaggtgaca accaccagca ggatcaccac cgctttgagg atggtcagca ggatctcaat
     8761 cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag atgcgcgcca
     8821 gccagcaccg gagcaatgcc ggacataccc atcggcaagc ccacctgccc tgccgtcagt
     8881 ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa ggagacgcgt
     8941 gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat gtacggctgc
     9001 ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg
     9061 taatacggcg caatacgcca tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg
     9121 aagtaatcca gaccattctc gctggtttca aacagacgca cgcccggatc gccaaagcgc
     9181 agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt ccagcccggt
     9241 gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc ttccatcgag
     9301 aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct gatattggcg
     9361 cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg ccagtttctg cccacgaata
     9421 cggaatgtcg catccggcgc agcatctttg atacctgcca gttccgggat tttcgccaca
     9481 acagcgtcaa tcacatggtc gagctgcgtc cagtccactt cacggctcag cagggtgctg
     9541 tgcagcgagt gcagccagcg ccagctttcc agcatgacag ttttgctgtc gtaataagcc
     9601 ggatcgtaaa cctggaagaa acgttgggcg cgaccttcat tattaattac cgtaccgtcg
     9661 ctttcagcaa agctggcggc ggaaagtacc agatgggcgt tttccatgat cgctgtacgc
     9721 tgatggtcaa ccaccatcac cagcggtgct ttagccagcg cagcattcac gcgggtagca
     9781 gaagcgtgac gatgcagatc gttttccaac accaccaccg cgtcggcgcg tccggtttcc
     9841 agttcggtta acgcttcttc aagcgaaccg ccacccataa tgcccagccc catgctgttg
     9901 acggaacggg caatcatggt gataccgacg tcagcgccgc gacctttcag ggctttcgcg
     9961 acgttagccg ccgcctgaat cacctctaag ctaccggcgt tcgtcccgga gataatcaac
    10021 ggtttcttcg cacctgccag tgcctgcacg atgacgtcga ttttgctttg cagctcaggt
    10081 tcgataccgt caaccgctgg tgcagagtta tccagcgcat gggcgatggc aaaacctaaa
    10141 cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg
    10201 tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt aaggattgcc
    10261 gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt acctttcacc
    10321 gcctgacgca ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc gcccagcacc
    10381 agtaccgcat cgtaagattc gatttcgcgc agagccggag tataaatgcc gccttcacgc
    10441 agcactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag
    10501 ttttcttcgc ccaccagttc acgcagtgca aagttgcttt ccacgctggc acgcggagaa
    10561 ccaataccga tcactttctt cgactgacgc agaatatctg ccgcgccctg cattgcctgt
    10621 tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg cggacgatcc
    10681 ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta gtggtttacc
    10741 gtaccgttgt aacggttttc gatacgacgc agttcgccgt aacgttcacc ggggctgatg
    10801 ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc ccatttacgg
    10861 ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg
    10921 ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg
    10981 tgcgcgccat aaacgcccag atctgtaccg tcagcgtaat ctttgtagta acgcacacag
    11041 cggtagcagg cgatgcagcg gttcatttcg tgagatatga atggccccaa atcctgatta
    11101 cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat cacggtcata
    11161 tcctgaagat ggcagttacc gccttcttca cataccggac agtcgtgcgg gtggttggtc
    11221 attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata
    11281 aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc
    11341 gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc cagcgccgga
    11401 tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag caggttgtcc
    11461 gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag catgcttcca
    11521 gttttctcgg tcagggaccg agcgttaatc gaaattcggt taccagcgct ctttcagcag
    11581 gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga ttcccgcctc
    11641 aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac caggtgcgtg
    11701 ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg tttcaatatc
    11761 gcccggctgg ccttcgccac gctccagcgc acgcagaatt ttcacgctcc acggcagacc
    11821 gtcgcggcac ggcgtacacc agccgcagga ctcacgggcg aaaaactctt ccaggttacg
    11881 caccagcgac accatgttga tctcatggtc aaccgccatc gccagcgccg tacccagacg
    11941 gctgcccgct ttaccgatac tttcgaattc catcggcaga tcaaggtgcg cttcggtcag
    12001 gaagtcagtc cccgcgccgc ctggctgcca ggctttaaat ttcagaccat cacgcatacc
    12061 accggcgtaa tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt cccacagtcc
    12121 cggatttttc acccgaccgg agaagcccat cagcttggtg ccagcatctt tacttttcga
    12181 gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac acagggtttc
    12241 gacgttgttg acacaggtcg gtttgcccca tacgccggag gttgccggga aggggggttt
    12301 cgagcgtggg ttagcacgac gcccttctag ggagttgatt aacgctgttt cttccccgca
    12361 gatgtagcgc cctgccccgg tatggacgaa cagttcgaag tcgaaacctg ttcccataat
    12421 gtttttgcca agcaagcccg cttcagtggc ttcggcaatg gcgcggcgca gatttactgc
    12481 cgcttcgata tattcgccac gcaggaagat gtagccacgg taagctttca gcgcaaacgc
    12541 ggagatgagc ataccttcca ccagcaggtg cggcagttgc tccatcaaca ggcggtcttt
    12601 ataggtgccc ggctccattt catcggcgtt acacagcagg taacggatgt tcatggattc
    12661 gtctttcggc atcaggctcc acttcaggcc agtagaaaag cccgctccgc cgcgcccttt
    12721 cagaccagcg tcttttacct gattaacgat ttcgtccgga gacagcccgg tcagcgcctt
    12781 acgcgcccct tcgtaaccgt ttttgctgcg gtattcgtcc agccacactg actgtttgtc
    12841 atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt ttttcattta
    12901 taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg
    12961 atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag cagcgtaaag
    13021 cggccatcaa atgtcgtttg ccctggtttg atgttcagct ttttctcgag cgccgcctga
    13081 ataccctgat acccgttgat atgacagacc acgctgtcgc aataacggat cacatggcga
    13141 ccaaccggct ggcggaagat ctgactgtag aacgttgcca caccttcgac gtcgcttgcc
    13201 ggaataccca gcacatcggc gatcgcgtgg atcgcaccat ccggcaccca gccacgctgc
    13261 ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc
    13321 tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt
    13381 tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata ctgcccagat
    13441 aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg
    13501 cataactcgg cgtgcggata cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt
    13561 agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg gcaggcatca
    13621 ccggccccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc
    13681 gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct tccggcatgt
    13741 tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact ttcagcatta
    13801 cgcgggtgta gcagtcagaa acaccaccac caaccgggat ttcaaagtcg aagttttcat
    13861 agccagaata aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg
    13921 cgccagtggt gccccactcc agcgcctctt tcgcgccata ggcggcaacg ccctgggaac
    13981 gacctttcag aatggtattt tgcagcgccg ctttctcgta agacgccaga cgtttcggca
    14041 tccagtcgag gaactcacgc agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc
    14101 cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc accagatcgt
    14161 aaattttctg acgatcggta aaggcgaaga acacgggcgt cattgcgccg acgtcctgaa
    14221 taaaggtcga gatgtacagc aggtggctgt tgatacggaa cagttcggag agcataacgc
    14281 gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc gccagcacgt
    14341 aaggcatttc gttaacgcag ccgccgaggt attcaatacg gtcggtatac ggaatgtagc
    14401 tgtgccagga ctggcgctcg cccattttct ccgcaccacg gtggtggtag ccgatgtctg
    14461 gtacgcagtc gacaatctct tcgccgtcga gttgtaaaac gatacggaaa gccccgtgcg
    14521 ccgacgggtg gttcggaccg aggttgagga acatgaagtc ctcgttttcg gtgccgcgct
    14581 tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc tgtttggctt
    14641 tggtcagctc aaacggcgag aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt
    14701 gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg tcgaaagtaa
    14761 tgccaaacag atcccaggtt tcacgctcat accagttagc gttcgggaac agtttggtga
    14821 aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg tcgcggttac
    14881 gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt
    14941 gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa ggtttcggca
    15001 gtttctttaa gaaatcgcca acttccagta attgttcacg cttgatccac acaacgggaa
    15061 ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg cgcagttcgc
    15121 caattaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc gcggttaagt
    15181 cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc
    15241 gaaatagatt taggaataag cgaagaaaaa tccctttgcc gacaggcgca aattaaatct
    15301 cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg
    15361 attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc ggacgacgtt
    15421 ctttgccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca ggacgcggcg
    15481 ggcagcccgg gatatacaca tcaaccggga tgaatttatc gacgccctgc acaacggaat
    15541 aaatatcgta cataccacca gagttggcac aggcacccat tgagataacc cattttggtt
    15601 ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag caggttcctg
    15661 caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg
    15721 ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag gaaagaccga
    15781 agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg agcttgccca
    15841 taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc tgcttttgca
    15901 gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa tccatcttaa
    15961 tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc atacgctcgc
    16021 ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa accagacctg
    16081 ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg ctttcgcgga
    16141 tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg aagaacatgg
    16201 ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag tcgataccgg
    16261 attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac caaccaccta
    16321 ccagcattag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat gcccagtgat
    16381 gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa gtagcgccag
    16441 attcactgct cttcacggca gatggacgcc acatcgattc atggggagga ataaaaaaaa
    16501 ccttacaatc actgtagaaa ttcttttata cagctaattg atgtggtctt ttactccttt
    16561 ctataacctt ttgtcaactt taacaaaagt ttcttcacat tagtttacat aatatcaaca
    16621 ccattagcat ttaatgccct ttcaccccag atccttgacg actccaggat aattagatgt
    16681 tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt
    16741 tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgtcat
    16801 tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt agcaggtgat
    16861 ttttttcagg cgattatttg tgcgttcggg acgtgaatct ctggtgattg aaaaatgaac
    16921 agttttgtac gttctgcact atgcgaggaa ggatttttac taaaaaaaag ccgctggggt
    16981 ttaaaacacc cccagcggct cgttttttac actattgtct caggaattat ctatcgtccg
    17041 tcgattactc gatatccctt tcaatcaaca atgaatcatc cccttccgga gcagacattg
    17101 ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc tgggccagct
    17161 cattattact ggacgggtca tagcacagca aatattcggt atcaggtagc ggcggtaagc
    17221 catctactcc actcagcaca cgcaggtccg agctcatcat ctcaaccggc cttgccgtca
    17281 cgccaagacc ggctttcact gccgcacgaa cggccggaag cgtcgaggcg acataagcca
    17341 gtcgccatgg aatatctgct ttattaagcg ttgccagcac catatcgcga aacggacttg
    17401 gatcatccag caatacaaga gggatcggct cgcctttttg caatacgtat tccgctgcgc
    17461 agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa ggacgatggg
    17521 tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag gcattacgtt
    17581 taacgcggac atccagcgct aatttcggat aaaccgaact cacgcgattt aacaggaaag
    17641 gtaagatcgt atcggcagat tcatctgaag caccgatagt taacacgccc tgaagattac
    17701 tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg gcgtaaccaa
    17761 gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg aacagttctt
    17821 tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga gtacgacaca
    17881 cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat gttctcagca
    17941 gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat cacttactgg
    18001 cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc atttgtaatg
    18061 tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata tgagttgcac
    18121 taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag aatatatctt
    18181 cagggatcgc atatctatta agttactcac tcttttctat ttatgacatg cgcgtgtttg
    18241 tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt catacgcgaa
    18301 cacgtacaaa cagcaatggt gaggcggcat caagagcagg gatccgtcat ttatgcgagc
    18361 attttacccc aaaaaccttt tatttataag ggccattgcg aatcatctga tgcaaagtta
    18421 tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt gaatattaaa
    18481 taataattaa ttagcatcat cctcattcat taattccgct taacaatagt ttcacacctt
    18541 gccccattac gacacctcgg aaatcatcac gctgttaaac tctccgtaat gcggaaatat
    18601 catcatcgcg tttcattagt gaattcttaa atgaggcatt ttcacacaat tatcttacag
    18661 ataaaaaaac cagacttaca attaagaatc agaacaattc actatataac attgcatgta
    18721 aagcatatac acctcattat tttgtcatca ttaagttatt aacagcacaa tcgagccttc
    18781 ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg agtacattca
    18841 cggcagggag tggcgataac agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc
    18901 cagcaaatta gagaatgtct gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg
    18961 cctggaagaa gaaggtaaca aggtactgaa actgaacatc ggcaacccag ccccgttcgg
    19021 ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc aacctgccta ccgctcaagg
    19081 gtattgcgat tccaaaggtc tttactccgc gcgtaaagcc atcatgcagc actaccaggc
    19141 tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg tatcggagct
    19201 tatcgttcag gcaatgcagg cattgctgaa cagcggggac gaaatgttgg ttcctgcacc
    19261 agattaccca ctatggaccg cggcggtttc gctttccagc ggtaaagcgg tgcattatct
    19321 ttgcgatgaa tcctctgact ggttcccgga cctcgatgat attcgcgcta aaattacgcc
    19381 tcgtacgcgt gggatcgtta ttatcaaccc aaataaccca accggcgcgg tatattccaa
    19441 agagctttta atggagattg tggagattgc acgtcagcat aatctcatta tcttcgccga
    19501 tgaaatttat gacaaaattc tttacgacga cgctgagcat cactcaattg cgccgctggc
    19561 acctgacctg ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg ttgcaggctt
    19621 ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct acatcgaagg
    19681 tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca
    19741 gaccgcgctg ggtggttatc agagcatcag tgaatttatt acccctggcg gtcgtcttta
    19801 tgagcagcgt aaccgcgcgt gggaactgat caacgatatt ccgggcgttt cctgcgtgaa
    19861 acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta acattcacga
    19921 cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg tgcaagggac
    19981 ggcattcaac tggccgtggc cggatcactt ccgcattgtc acgctaccgc gtgtcgatga
    20041 tatcgagctg tctttgagca agttcgcgcg tttcctttct ggttatcatc agctgtaatc
    20101 ttaatttcac tgccggagat tgcatccggc agcgttatcc cgccacaatg acctgatgat
    20161 gtcatcatac gtaaggtcac tatgaaacag agccattttt ttgcccatct ctcccgcctg
    20221 aaactcatta accgctggcc gctaatgcgc aacgtgctga cggaaaatgt gtccgaacac
    20281 agtttgcagg tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg aaaatttggc
    20341 ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt accacgatgc ctcagaagtg
    20401 ctcaccggcg atctccctac tccggtgaaa tacttcaatt cgcaaatcgc tcaggagtac
    20461 aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata tggttccgga agagctgcgg
    20521 gatatctttg cgccgttaat tgacgagcat gcatatagcg atgaagaaaa atcgctggtg
    20581 aaacaggcag atgcactgtg tgcatatctg aaatgtctgg aagaactcgc ggccggaaat
    20641 aatgaattct tgctggcaaa aacgcgactg gaagcgacgc ttgaagcgcg tcgcagccag
    20701 gagatggact acttcatgga agtatttgtt cccagcttcc atctttcgct cgatgagatt
    20761 agccaggatt caccgctgta agcagccgga gtctgcgtcg catcaggcaa taagcgccgg
    20821 atgcgacatc aggctcttgt caaaacggaa acagcatcgg gatcatcacc acacaaaccg
    20881 ccatcacgat aatggtgaac ggtaccccca acttcacaaa gtcactgaag ctgtaatttc
    20941 ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg tgtcataaag gcggcggatg
    21001 ctgccatcgc cacgaccatc gcgaatggat aaggcgacac ccccatcgtt ttggcagcag
    21061 ccagcgcaat cggagccatc aacaccgccg tcgcggtatt agagataaat agcccaataa
    21121 ccgccgacaa gacaaacaaa caccccagca tcatatgtgg cccgtaacca ccgccaatgt
    21181 ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc acagcaaacg
    21241 gcatcatccc aacgatcaaa ataatgctcg gccagtgaat ggatttatag gcgctttcag
    21301 catctataca gcggaatttc cccatcagca ggcaggcgat gatagcggca acaggattag
    21361 gaatttcatc tgtcagcatt aacgccacca ttagcaccag acagaaaatg gcatggggtg
    21421 cctggctgtg cgcgggcgat gcttcactca cctcttccgg taagttcagc gctacgaagt
    21481 cgcggccctg tttggccagc ataccgatca gtttccagtt acccacaacc aggatgatat
    21541 cgcccagcag cagaggctca tccgccagcg aaccttccag cgccacgcca ttgcgcttta
    21601 gccccaccac attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa
    21661 tcagttctga ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg gcctggtcag
    21721 aaaaatactc gccgcgcagt accatcggct ccagcaattg ctcactacaa aattgccgga
    21781 gatcgacatc agccgcagac atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa
    21841 ccccattaac gttcacgata acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt
    21901 tagcgccata acgctcacgt aatttgagat catccagccg ttgaccaatc attggcgatc
    21961 cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc
    22021 gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg ggtatcccct ttcagcatga
    22081 aacgcatcac taacatatac aaaataccca gcaccagcac gaccaggcca acaggtgtta
    22141 cgctaaagaa actgaagccg tgatagcctt cacgcagcaa ttcactgttg actaccaggt
    22201 tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag cccggcaaaa cttaacggca
    22261 tcatcagacg tgacggcgaa gtttgcatac gcatggcaac gcttaacacc acgggaataa
    22321 agatagcgac aacgccggtt gaactcataa acgcgccaag ccccgcgacg gtcagcatca
    22381 acaaaaccaa cattttgatt tcactgttgc ccgccacttt gaccagccat gttcccatta
    22441 cggtggcaac accggtacgg accaaaccat cgccaataat aaacaaggcg gcaatcagga
    22501 caacgttagg atcagaaaag ccggaaaata cttcggggac cgtcagcgtt ccgcttaatg
    22561 caaacgcgac aataacaaac aaagcgaccg catccatacg cactctgccc gtcgcaaaca
    22621 agacgatggc aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc
    22681 cttgttaatt gagggggatg acttgattct gccataaaaa agccccgacg agacggggct
    22741 aaatcatgat caggtgtttc actgaataat aacatcgcca tttagctgtt tggtcacagt
    22801 aatttgctcc agactgtgga ggaccaaatc gacctcattc aggcgcgggg tatctgccgg
    22861 agcgttaacc gcaatgacat ggcaacccgc cgccaggcca gaaagcacgc cagcgggagt
    22921 atcttccgcc accacacact cctgcggcgc aagccccagc agctgcgcac ctaacagata
    22981 cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg gttacaaaca cctctggtgc
    23041 gggaagccca gctattttat ggcgcgctcg cgctaccggc atggagccag aagtcacaat
    23101 ggcccacgga atacctgctt tattcaaatg attgagtaag gcgattgccc ccggaagcgc
    23161 ggtaatacct tcggtttccg tggcctcgat gtgctccaga cacgtaaact cggcggcaat
    23221 atcagcctcg gatttgcccg ccataaaatg gcgcaaagag gtgatcgcct gtttaccgtg
    23281 aatgaaagcc agcacctctt ccggcgctaa cccatgacgt ctggcccagt tgctccacgc
    23341 ccgttctacc gcaggcaggg aatccaccag cgttccatca agatcaaaca gaaaaccttt
    23401 gcaccgcacg cgggcctcct caggcgttga taatttgatt aatttcgttg gcgctcaaat
    23461 ggtactgacg caggcaggca tgccacacat taagcatgcg ctgatatttt tcccacattg
    23521 gcgtctgggc gttaaaacca tgagtcccag cgtcaaaatg ggtatagcgc ccttccacat
    23581 taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga
    23641 aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccag gaaacatgca
    23701 acgcatgata catctccata atgtcgatga tagtgcggca ggtttcttct ttcagctcgc
    23761 caaactcgcg atccaattca cgcatctgta atccgtaacc acgctcaata attgtttgca
    23821 ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg tactgattag
    23881 acaaaataag acgttgcgcg ttggtcattt ccattgttga ctcctgtatc actctactac
    23941 ggtgaaaaaa aaagaaggct gagtatgcct tcttttatat gcgtaatcag gggtcaatta
    24001 caaatcatca aggaaagttt tatccagttg tttgaaggcg cgcttaagcg tgtcagctaa
    24061 tgcctggtaa tcaggcttgc cttcaacggg tgccaacacc tgtccagact cctgcaattt
    24121 accgcgaact tcataaaacc agttgaggat agcagggggt aatggcgtta cagaacgctt
    24181 gcccagccac cataatccct gcatgggtaa acttaaggcg aacagcgcag tggcaactgc
    24241 cggcccaagc tgaccgccca gggcaatctg ccagcagaga gtaaatacgg cgatcggcgg
    24301 cataaaacgg atcgcataac gcgtcatctt gataacgcga ttttcgacaa agaccggggc
    24361 aaggcgtttt tccagcggcc acgtctttga gtaatgctgt ccccggcgaa acaagctaaa
    24421 aaaattaaca gaacgattat ccggcgttga catgcttcac ctcaacttca catataaaga
    24481 ttcaaaaatt tgtgcaaatt cacaactcag cgggacaaca ttcaaaacat tttgtcttcc
    24541 atacccacta tcaggtatcc tttagcagcc tgaaggccta agtagtacat attcattgag
    24601 tcgtcaaatt catatacatt atgccattgg ctgaaaatta cgcaaaatgg catagactca
    24661 agatatttct tccatcatgc aaaaaaaatt tgcagtgcat gatgttaatc ataaatgtcg
    24721 gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt tttagccacg tatcaattat
    24781 aggtacttcc atgtcgagta agttagtact ggttctgaac tgcggtagtt cttcactgaa
    24841 atttgccatc atcgatgcag taaatggtga agagtacctt tctggtttag ccgaatgttt
    24901 ccacctgccc gaagcacgta tcaaatggaa aatggacggc aataaacagg aagcggcttt
    24961 aggtgcaggc gccgctcaca gcgaagcgct caactttatc gttaatacta ttctggcaca
    25021 aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac acggcggcga
    25081 aaagtatacc agctccgtag tgatcgatga gtctgttatt cagggtatca aagatgcagc
    25141 ttcttttgca ccgctacaca acccggctca cctgatcggt atcgaagaag ctctgaaatc
    25201 tttcccacag ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc accagactat
    25261 gccggaagag tcttacctct acgccctgcc ttacaacctg tacaaagagc acggcatccg
    25321 tcgttacggc gcgcacggca ccagccactt ctatgtaacc caggaagcgg caaaaatgct
    25381 gaacaaaccg gtagaagaac tgaacatcat cacctgccac ctgggcaacg gtggttccgt
    25441 ttctgctatc cgcaacggta aatgcgttga cacctctatg ggcctgaccc cgctggaagg
    25501 tctggtcatg ggtacccgtt ctggtgatat cgatccggcg atcatcttcc acctgcacga
    25561 caccctgggc atgagcgttg acgcaatcaa caaactgctg accaaagagt ctggcctgct
    25621 gggtctgacc gaagtgacca gcgactgccg ctatgttgaa gacaactacg cgacgaaaga
    25681 agacgcgaag cgcgcaatgg acgtttactg ccaccgcctg gcgaaataca tcggtgccta
    25741 cactgcgctg atggatggtc gtctggacgc tgttgtattc actggtggta tcggtgaaaa
    25801 tgccgcgatg gttcgtgaac tgtctctggg caaactgggc gtgctgggct ttgaagttga
    25861 tcatgaacgc aacctggctg cacgtttcgg caaatctggt ttcatcaaca aagaaggtac
    25921 ccgtcctgcg gtggttatcc caaccaacga agaactggtt atcgcgcaag acgcgagccg
    25981 cctgactgcc tgatttcaca ccgccagctc agctggcggt gctgttttgt aacccgccaa
    26041 atcggcggta acgaaagagg ataaaccgtg tcccgtatta ttatgctgat ccctaccgga
    26101 accagcgtcg gtctgaccag cgtcagcctt ggcgtgatcc gtgcaatgga acgcaaaggc
    26161 gttcgtctga gcgttttcaa acctatcgct cagccgcgta ccggtggcga tgcgcccgat
    26221 cagactacga ctatcgtgcg tgcgaactct tccaccacga cggccgctga accgctgaaa
    26281 atgagctacg ttgaaggtct gctttccagc aatcagaaag atgtgctgat ggaagagatc
    26341 gtcgcaaact accacgctaa caccaaagac gctgaagtcg ttctggttga aggtctggtc
    26401 ccgacacgta agcaccagtt tgcccagtct ctgaactacg aaatcgctaa aacgctgaat
    26461 gcggaaatcg tcttcgttat gtctcagggc actgacaccc cggaacagct gaaagagcgt
    26521 atcgaactga cccgcaacag cttcggcggt gccaaaaaca ccaacatcac cggcgttatc
    26581 gttaacaaac tgaacgcacc ggttgatgaa cagggtcgta ctcgcccgga tctgtccgag
    26641 attttcgacg actcttccaa agctaaagta aacaatgttg atccggcgaa gctgcaagaa
    26701 tccagcccgc tgccggttct cggcgctgtg ccgtggagct ttgacctgat cgcgactcgt
    26761 gcgatcgata tggctcgcca cctgaatgcg accatcatca acgaaggcga catcaatact
    26821 cgccgcgtta aatccgtcac tttctgcgca cgcagcattc cgcacatgct ggagcacttc
    26881 cgtgccggtt ctctgctggt gacttccgca gaccgtcctg acgtgctggt cgccgcttgc
    26941 ctggcagcca tgaacggcgt agaaatcggt gccctgctgc tgactggcgg ctacgaaatg
    27001 gacgcgcgca tttctaaact gtgcgaacgt gctttcgcta ccggcctgcc ggtatttatg
    27061 gtgaacacca acacctggca gacctctctg agcctgcaga gcttcaacca ggaagttccg
    27121 gttgacgatc acgaacgtat cgagaaagtt caggaatacg ttgctaacta catcaacgct
    27181 gactggatcg aatctctgac tgccacttct gagcgcagcc gtcgtctgtc tccgcctgcg
    27241 ttccgttatc agctgactga acttgcgcgc aaagcgggca aacgtatcgt actgccggaa
    27301 ggtgacgaac cgcgtaccgt taaagcagcc gctatctgtg ctgaacgtgg tatcgcaact
    27361 tgcgtactgc tgggtaatcc ggcagagatc aaccgtgttg cagcgtctca gggtgtagaa
    27421 ctgggtgcag ggattgaaat cgttgatcca gaagtggttc gcgaaagcta tgttggtcgt
    27481 ctggtcgaac tgcgtaagaa caaaggcatg accgaaaccg ttgcccgcga acagctggaa
    27541 gacaacgtgg tgctcggtac gctgatgctg gaacaggatg aagttgatgg tctggtttcc
    27601 ggtgctgttc acactaccgc aaacaccatc cgtccgccgc tgcagctgat caaaactgca
    27661 ccgggcagct ccctggtatc ttccgtgttc ttcatgctgc tgccggaaca ggtttacgtt
    27721 tacggtgact gtgcgatcaa cccggatccg accgctgaac agctggcaga aatcgcgatt
    27781 cagtccgctg attccgctgc ggccttcggt atcgaaccgc gcgttgctat gctctcctac
    27841 tccaccggta cttctggtgc aggtagcgac gtagaaaaag ttcgcgaagc aactcgtctg
    27901 gcgcaggaaa aacgtcctga cctgatgatc gacggtccgc tgcagtacga cgctgcggta
    27961 atggctgacg ttgcgaaatc caaagcgccg aactctccgg ttgcaggtcg cgctaccgtg
    28021 tttatcttcc cggatctgaa caccggtaac accacctaca aagcggtaca gcgttctgcc
    28081 gacctgatct ccatcgggcc gatgctgcag ggtatgcgca agccggttaa cgacctgtcc
    28141 cgtggcgcac tggttgacga tatcgtctac accatcgcgc tgactgcgat tcagtctgca
    28201 cagcagcagt aatctcgtca tcatccgcag ctttgcgctg cggatatctg aaccggaaat
    28261 aatcactatt tccggttttt tattctctta atctgcatta atcctttctg attatcttgc
    28321 ttaactgcgc tgcatcaatg aattgcgcca tttcactttg catacttacc actttgtttt
    28381 gtgcaaggga atatttgcgc tatgtccgca atcactgaat ccaaaccaac aagaagatgg
    28441 gcaatgcccg atacgttggt gattatcttt tttgttgcta ttttaaccag ccttgccacc
    28501 tgggtagttc cggtggggat gtttgacagt caggaagtgc agtatcaggt tgatggtcaa
    28561 acaaaaacac gcaaagtcgt agatccacac tcatttcgca ttctgactaa cgaagcaggc
    28621 gaacctgagt atcaccgcgt acagctgttc acgacgggcg atgaacgccc gggcctgatg
    28681 aacttcccgt ttgaaggatt aacctcagga tcgaaatacg ggacagctgt tggcatcatc
    28741 atgtttatgc tggtgattgg cggcgcgttt ggcattgtga tgcgtacagg aaccattgat
    28801 aacggtatcc tggcgcttat tcgccatacc cgcgggaatg aaattctctt tattcctgcg
    28861 ctgtttattc tgttttcact tggcggcgcg gtatttggta tgggagaaga ggccgtcgcc
    28921 tttgccatta tcatcgcacc gctaatggtc cggctgggct atgacagtat taccaccgtc
    28981 ctggtgacct atattgccac gcaaatcggt tttgccagtt cgtggatgaa cccgttttgt
    29041 gtggtcgttg ctcaggggat tgccggcgtt ccggtgcttt ctggctccgg gttgcgcatc
    29101 gtggtgtggg ttatcgccac tatgattggc ctgatcttta ccatggtgta cgcctcacga
    29161 gtgaaaaaga atcctcttct gtcacgcgtg catgagtccg accgcttctt tcgtgaaaag
    29221 caggcggatg ttgaacaacg tccgtttacc tttggtgact ggctggtatt gattgtcctg
    29281 accgccgtaa tggtctgggt gatttggggc gtgatcgtta atgcctggtt tattccagaa
    29341 attgccagcc agttcttcac catgggtctg gtgattggca tcatcggcgt tgttttccgc
    29401 cttaacggca tgacggttaa taccatggct tcatccttta ccgaaggggc gcgaatgatg
    29461 atcgcccctg ccctgctggt gggtttcgcc aaagggattt tgctgctggt cggtaatggt
    29521 gaagcgggtg atgccagcgt gttaaatacc atcctcaaca gcattgccaa tgccattagc
    29581 ggtctggaca acgcggtcgc ggcctggttt atgttgctct tccaggcggt atttaatttc
    29641 ttcgtgacgt ccggttctgg tcaggcggcg ttaaccatgc cgttactggc accgcttggc
    29701 gatctggtcg gtgttaaccg tcagattacc gtgctggcat tccagtttgg tgatggcttc
    29761 agtcacatca tttacccaac ctcagcttcg ttaatggcaa ctctcggtgt gtgcagggtg
    29821 gacttccgta actggctgaa ggtgggcgcg accctacttg gactgctgtt tattatgtcc
    29881 agcgtcgtag tgatcggcgc tcagttgatg ggctaccact aaaaatgtta agagccgcaa
    29941 ttgcggctct tttttcattc tgccgtttca gtctctacgg cttcattttt ggcattgcgc
    30001 ttcatccaca acgccagcgc tttcagcgag tctggagtga actcatcgca gcgtgcggtg
    30061 atttcttccg gcgtcagcca gcaaacttca ctgacttcat cttcctgtag ggcgaaggga
    30121 ccgtgagaga cgcagctgaa caacgcgccc cagacacggc aatttttatc ttcgaaatag
    30181 aactgcccgt gctcggcaaa ggggacaccg gcaatgccca actcttcttc cgcttcgcga
    30241 cgcgcggatt ccagcagttg ctcatcggcc tgaaccacac cgcctgcggt cgcatctaac
    30301 atgccgggta aaaagtcttt tgtctcggta cgacgctgga ccagaatttt gcccatgcca
    30361 tcatgcacga cgatgtaagt tgcacgatga cgcagacact gtgcccgcat ttgttcccgg
    30421 ctggcttgtg caatgacttc gttctcttca ttgacaatat ccacccattc agtacttgcc
    30481 aaacgacgct gttccattat caggaaacct tctttttctg gcgctcttac ggcgcatttt
    30541 gagttgtggg taaattacgg attaatcgcg acctgcgcaa tgatactttg atcattgagt
    30601 gcgattacac taagtacgtc attatccagc atgccataac tcgccggatt accgcctttc
    30661 ggaatactca ccgagccggg gttgaagtgg aaaatctccc cccgctgttc tgccactggc
    30721 agatgggtat gaccgtacac cagcacatcg ttctggttta aagcaggtag attttccggg
    30781 ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt ccagtaatac ctgttgccac
    30841 ggcgcggtta tcgggaaatg cagcagcatt tgatccactt cgctgtcgca gttgccgcgc
    30901 acagcgataa ccttatgtgc cacttcatta agccgttcag cgactttggc tggcgcgtaa
    30961 ccctccggta aagcattacg cgggccgtga ttcaacacgt cgccaaggat caccagccac
    31021 tgggcaccgc tttgggcaaa caactccaga acacgttccg tcgccggtaa cgacccatga
    31081 atgtccgatg caaacatcag tttcatcact cactcctcgt cgaaaaaacg taagctatga
    31141 tactggattc tgtcgttgct atcagccaga ttgcttcgcc gaaagtgcaa taaaacgctg
    31201 gacagacgct cgctgccact ggaatgtcgc ataatccacc aggcgttctg gcacctcatc
    31261 gccatgtagt accaggcggt taatcattag cgccagatca gtatcagcaa tgcaccattc
    31321 accaaataaa ttcggctgac ccagtgctaa caaatgttct gccatcgcga cactggcttt
    31381 tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg tcggacgctc
    31441 ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg cacgcgcacg
    31501 attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag
    31561 atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta ataacggcac
    31621 acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc tgtcgaggtc
    31681 gatggtcttg atatgaaacg acaggccttt ttcctgcaac gccacccaag cggataacac
    31741 ataaggggag aaaaagtggg catctgacca aagcgtgata gcgggtttac tcattatgtc
    31801 ctcggcaata aaggaggctg gctatcaaac tatagagcct ttttgtcact gtcacgtgac
    31861 gaatactcac gtaagtcgcc gcgctttcta tacttgtggt gttcagatga ttaaacaacc
    31921 ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa aattacgctg
    31981 tttctcgaag aagcagagct tgattatcgc ttgattaagg tagacctggg gaaaggcggt
    32041 cagtttcgtc cggaattttt gcgcatttcg cccaacaaca aaattccggc aattgttgat
    32101 cattctcctg ccgatggcgg cgaaccgcta agcctctttg aatctggtgc cattttgttg
    32161 tatctggctg agaaaacggg gctctttttg agtcatgaaa cgcgtgaacg cgccgccaca
    32221 ttacagtggt tattctggca ggtaggcgaa ctggggccga tgcttgggca aaatcatcat
    32281 tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca ggttgaaact
    32341 cagcgtcttt accatgtact aaacaagcgg ctggaaaact cgccctggct gggaggcgag
    32401 aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac tcgccagcga
    32461 attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg ttcgcgccct
    32521 gccaccgggc aggcactgct aaaagcacaa ctcggtgatg agcgttcgga tagttaacag
    32581 aaacaggttc tcgtgtatta tttcatccta agtaaaacaa cggagaacct gcaatggcac
    32641 aacctgccgc tattattcgt ataaagaacc ttcgtttacg tacgtttatc ggaattaagg
    32701 aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac taccccgccg
    32761 ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa
    32821 acattattca gcatgtagag aataaccgtt tctctttgtt ggaaaaatta actcaggatg
    32881 tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag atcgataaac
    32941 tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag cgttaaccgc
    33001 catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa ttggtcgcca
    33061 tttgattccg cgtttgctgg agctgggcca tcaaatcacg gtagtgacgc gtaacccaca
    33121 gaaagccagt tccgttctcg gccctcgggt gacgctatgg caagggctag ccgatcaaag
    33181 caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg ctgataaacg
    33241 ctggactcac gagcaaaaag agcgtctctg ccaaagccgc tggaatatca cgcaaaaact
    33301 ggtcgatttg attaatgcca gcgacacgcc gccgtcggta ctcatttccg gctcggcaac
    33361 gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga
    33421 atttactcat aaactctgcg cccgctggga agagattgcc tgccgggcgc aaagtgacaa
    33481 aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca ccggacggcg gtattctcgg
    33541 taaaatgctg ccaccgtttc gtcttggcct gggcgggccg attgactctg gtcggcagta
    33601 tctggcctgg attcatattg atgatatggt caacggtatt ctctggctgc tggataacga
    33661 gctgcgcggg ccatttaata tggtttcgcc ctacccggta cgcaatgaac aatttgccca
    33721 tgcgctcggt cacgcgctgc atcgcccggc cattttgcgc gtccccgcga ccgccattcg
    33781 actgttaatg ggcgaatctt cagtactggt attaggtgga caacgcgcgc tgcctaaacg
    33841 gctggaagaa gcgggttttg cgtttcgctg gtacgattta gaagaggcgc tggcggatgt
    33901 cgttcgctga tgtggtttac agcaaacatc cgccagttaa ctcccggtgt tacaggatta
    33961 gtggctttgc gcgataagat cgtctggtga aagtcgggtc accatcataa ctaactctct
    34021 gtctaaacct ctatccagca tctcctgagc aatacgcagg gcttcttcgt gtttgccctg
    34081 cattgcgcct tcttcacgta atctgtcagc aatggtcatc agtttctcct tttcttgtgg
    34141 tgcgcgttcc gctatctcac caataaatgc acgaaaacgc tgggcatccc ctgtttgtaa
    34201 tacgtaatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa ctagcagcga
    34261 aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct gaattaactc
    34321 caacagcgcc attttgcggt gttgcataat ctcgtcatcc ggcaccacgg taatatccac
    34381 caacggaaac gctgatgaat atattttgcg ggctatagca ggctcggcaa attcatcaag
    34441 ccagcagagt gaataaggat aaggacttct gcaaccatga taaaacagca tcgggatcac
    34501 caatggaagc tctttatagc ccg
//
LOCUS       DADLTD010000017        33997 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLTD010000017 DADLTD010000000
VERSION     DADLTD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.017"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(239..451)
                     /gene="rpmE"
                     /locus_tag="JN701_000658"
     CDS             complement(239..451)
                     /gene="rpmE"
                     /locus_tag="JN701_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY7363192.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            654..2852
                     /gene="priA"
                     /locus_tag="JN701_000659"
     CDS             654..2852
                     /gene="priA"
                     /locus_tag="JN701_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY7363193.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPEEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3008..4033
                     /gene="cytR"
                     /locus_tag="JN701_000660"
     CDS             3008..4033
                     /gene="cytR"
                     /locus_tag="JN701_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY7363194.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4125..5084
                     /gene="ftsN"
                     /locus_tag="JN701_000661"
     CDS             4125..5084
                     /gene="ftsN"
                     /locus_tag="JN701_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY7363195.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5177..5707
                     /gene="hslV"
                     /locus_tag="JN701_000662"
     CDS             5177..5707
                     /gene="hslV"
                     /locus_tag="JN701_000662"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY7363196.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5717..7048
                     /gene="hslU"
                     /locus_tag="JN701_000663"
     CDS             5717..7048
                     /gene="hslU"
                     /locus_tag="JN701_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY7363197.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7115..8041
                     /gene="menA"
                     /locus_tag="JN701_000664"
     CDS             7115..8041
                     /gene="menA"
                     /locus_tag="JN701_000664"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY7363198.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8134..8619
                     /gene="rraA"
                     /locus_tag="JN701_000665"
     CDS             8134..8619
                     /gene="rraA"
                     /locus_tag="JN701_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY7363199.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8704..8949)
                     /gene="zapB"
                     /locus_tag="JN701_000666"
     CDS             complement(8704..8949)
                     /gene="zapB"
                     /locus_tag="JN701_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY7363200.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9375..10220
                     /gene="glpF"
                     /locus_tag="JN701_000667"
     CDS             9375..10220
                     /gene="glpF"
                     /locus_tag="JN701_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAY7363201.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYHKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10243..11751
                     /gene="glpK"
                     /locus_tag="JN701_000668"
     CDS             10243..11751
                     /gene="glpK"
                     /locus_tag="JN701_000668"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY7363202.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11886..12896
                     /gene="glpX"
                     /locus_tag="JN701_000669"
     CDS             11886..12896
                     /gene="glpX"
                     /locus_tag="JN701_000669"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7363203.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12993..13739
                     /gene="fpr"
                     /locus_tag="JN701_000670"
     CDS             12993..13739
                     /gene="fpr"
                     /locus_tag="JN701_000670"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY7363204.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13744..14172)
                     /gene="uspD"
                     /locus_tag="JN701_000671"
     CDS             complement(13744..14172)
                     /gene="uspD"
                     /locus_tag="JN701_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY7363205.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14199..14498)
                     /locus_tag="JN701_000672"
     CDS             complement(14199..14498)
                     /locus_tag="JN701_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY7363206.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14710..15150)
                     /locus_tag="JN701_000673"
     CDS             complement(14710..15150)
                     /locus_tag="JN701_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7363207.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15251..15850
                     /locus_tag="JN701_000674"
     CDS             15251..15850
                     /locus_tag="JN701_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY7363208.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15958..16725
                     /gene="tpiA"
                     /locus_tag="JN701_000675"
     CDS             15958..16725
                     /gene="tpiA"
                     /locus_tag="JN701_000675"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY7363209.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16780..17535)
                     /gene="cdh"
                     /locus_tag="JN701_000676"
     CDS             complement(16780..17535)
                     /gene="cdh"
                     /locus_tag="JN701_000676"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY7363210.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17642..18631)
                     /locus_tag="JN701_000677"
     CDS             complement(17642..18631)
                     /locus_tag="JN701_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7363211.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLAMAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18951..19913)
                     /gene="pfkA"
                     /locus_tag="JN701_000678"
     CDS             complement(18951..19913)
                     /gene="pfkA"
                     /locus_tag="JN701_000678"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY7363212.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20094..20996)
                     /gene="fieF"
                     /locus_tag="JN701_000679"
     CDS             complement(20094..20996)
                     /gene="fieF"
                     /locus_tag="JN701_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY7363213.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21144..21656)
                     /gene="cpxP"
                     /locus_tag="JN701_000680"
     CDS             complement(21144..21656)
                     /gene="cpxP"
                     /locus_tag="JN701_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY7363214.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21806..22504
                     /gene="cpxR"
                     /locus_tag="JN701_000681"
     CDS             21806..22504
                     /gene="cpxR"
                     /locus_tag="JN701_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY7363215.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22501..23874
                     /gene="cpxA"
                     /locus_tag="JN701_000682"
     CDS             22501..23874
                     /gene="cpxA"
                     /locus_tag="JN701_000682"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY7363216.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23980..24654)
                     /gene="yiiM"
                     /locus_tag="JN701_000683"
     CDS             complement(23980..24654)
                     /gene="yiiM"
                     /locus_tag="JN701_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY7363217.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24803..25789)
                     /gene="kdgT"
                     /locus_tag="JN701_000684"
     CDS             complement(24803..25789)
                     /gene="kdgT"
                     /locus_tag="JN701_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY7363218.1"
                     /translation="MQIKRSIEKIPGGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGM
                     ITGTVSILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGV
                     EVGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILG
                     TAGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLT
                     VIAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAE
                     MVPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26049..26669)
                     /gene="sodA"
                     /locus_tag="JN701_000685"
     CDS             complement(26049..26669)
                     /gene="sodA"
                     /locus_tag="JN701_000685"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY7363219.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26953..27987
                     /gene="rhaT"
                     /locus_tag="JN701_000686"
     CDS             26953..27987
                     /gene="rhaT"
                     /locus_tag="JN701_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HAY7363220.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27984..28922)
                     /gene="rhaR"
                     /locus_tag="JN701_000687"
     CDS             complement(27984..28922)
                     /gene="rhaR"
                     /locus_tag="JN701_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY7363221.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVSFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(28906..29742)
                     /gene="rhaS"
                     /locus_tag="JN701_000688"
     CDS             complement(28906..29742)
                     /gene="rhaS"
                     /locus_tag="JN701_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY7363222.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30030..31442
                     /gene="rhaB"
                     /locus_tag="JN701_000689"
     CDS             30030..31442
                     /gene="rhaB"
                     /locus_tag="JN701_000689"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY7363223.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31439..32698
                     /gene="rhaA"
                     /locus_tag="JN701_000690"
     CDS             31439..32698
                     /gene="rhaA"
                     /locus_tag="JN701_000690"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY7363224.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQHG"
     gene            32873..33697
                     /gene="rhaD"
                     /locus_tag="JN701_000691"
     CDS             32873..33697
                     /gene="rhaD"
                     /locus_tag="JN701_000691"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY7363225.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33707..>33997
                     /gene="rhaM"
                     /locus_tag="JN701_000692"
     CDS             33707..>33997
                     /gene="rhaM"
                     /locus_tag="JN701_000692"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY7363226.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8339 a         8867 c         8812 g         7979 t
ORIGIN      
        1 gcaccccggc tgaaccctga acaatgctac cgccatactg cgtcatgtcg ccctgacgcg
       61 ccgccggttt tccgctcatc gtaaaatcct tttgaaataa ataagaacca gaagcctagc
      121 gccagtcaac aacaggaaat gataatgagt taacaccccg gaatttttca gaatttataa
      181 aaggcaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt
      241 atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa
      301 catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg aggttcaggt
      361 catgaccaac agtggagcgg attttcatta cgttaccgca agagcagcta gcagtaattt
      421 cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc
      481 tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca atacgatcat
      541 ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact gatccgcact
      601 cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat gctatgcccg
      661 ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg ctgccagaag
      721 gcatgacggt taaagccggg tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca
      781 tcggggttgt ggtgtcagtt agcgatgtca gtgagctgcc gcttaacgag ctgaaagcgg
      841 tagtggaagt gctggacgtt gagccggtat ttactcactc cgtctggcgg ttactgctct
      901 gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc ttgccgattt
      961 tactgcgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt gccactgaac
     1021 aaggccaggc ggtggatctg aacagcctga aacgctcccc caagcaacaa caggcgctgg
     1081 cggcgttacg gcaaggcaaa atctggcgcg accaggtcgc cacgctcgaa tttaatgatg
     1141 ccgcgttgca ggcgctacgc aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt
     1201 ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg aataccgaac
     1261 aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc tggctgctgg
     1321 cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa aacgtgctcg
     1381 ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg caaactatcg
     1441 cccgttttcg tgaacgtttt aatgcccccg aggaagttct gcattccggc ctgaacgaca
     1501 gcgagcggct ttctgcgtgg ttgaaagcga aaaatggcga ggcggcgatt gtgatcggca
     1561 cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt gatgaagagc
     1621 acgacagctc ctacaagcag caggaaggct ggcgctatca tgcccgcgac ctggcggtgt
     1681 atcgtgcgca cagcgagcaa atcccgatta ttcttggctc cgcaacgccc gcgctggaaa
     1741 cgttatgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacccgt cgggcaggga
     1801 atgctcgtcc ggcaattcaa catgtgctgg atttaaaagg tcagaaggtg caggcaggtc
     1861 tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat caggttatcc
     1921 tctttcttaa tcgtcggggt tttgcgcctg cgctgctgtg ccacgactgc ggctggatag
     1981 ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag catttgcgct
     2041 gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc
     2101 acctggtccc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg ccgatgttcc
     2161 ccggcgtgcc catttcacgt atcgaccgcg ataccaccag ccgcaaaggg gcactggaac
     2221 agcaactggc ggaagtgcat cgcggcggcg cgaggatttt gatcggcaca caaatgctgg
     2281 cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc
     2341 tgttttctgc cgattttcgc tcggcagaac gtttcgccca gctttacacc caggtcgccg
     2401 gtcgtgccgg gcgtgcgggt aaacagggcg aagtggtgct gcaaacgcac catccggaac
     2461 atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa caggcgctgg
     2521 ctgagcgacg catgatgcag ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag
     2581 atcataacaa tcagcacgcg ccgctgttcc tgcaacaact gcgtaatctg atcctttcca
     2641 gcccgctggc agacgacaaa ctgtggattc tcggcccggt tccggctctg gcacctaagc
     2701 gtggtggccg ctggcgctgg cagatcttgt tgcagcaccc ttcccgcgtg cgcttgcaac
     2761 acatcattag tggtacgctg gcgcttatta atacaatacc ggattcccgc aaggtgaaat
     2821 gggtgttgga tgtcgatccc atcgaaggct aatgcgcaaa cgttgcgagg cggatcgaaa
     2881 aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg cgctatctgc
     2941 taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgta ccaggcgagg
     3001 agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa
     3061 ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata aagtctccca
     3121 ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac cgcagcctat
     3181 ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc cggatatctg
     3241 cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa atcacggata
     3301 tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt
     3361 gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc
     3421 cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat ttgcaccgga
     3481 actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg cagtaaatta
     3541 tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag agatgccact
     3601 gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa ttatggttga
     3661 tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag caatgcaaca
     3721 gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg tgatggcgct
     3781 cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc tttccataat
     3841 cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca
     3901 gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa tgcaggggca
     3961 acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg gatcaacacg
     4021 cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa
     4081 gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca caacgagatt
     4141 atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc
     4201 aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc gttcttgtga
     4261 cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc gagacgctgc
     4321 aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc tggcgctaca
     4381 ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg
     4441 gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt gaacaaatgc
     4501 aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat gaacagacgc
     4561 cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc
     4621 aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag acgcgtacgt
     4681 cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct actcagcagc
     4741 cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag
     4801 ctgcaccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac
     4861 gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag acggtacgtg
     4921 ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc tggaatcgtg
     4981 tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat cggttgaaga
     5041 tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct caaaatcccc
     5101 cccatctata attgcattat gcctcgtact tttgtacggg gtttgtactc tgtattcgta
     5161 accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc atgtggtcat
     5221 cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt
     5281 ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg cggatgcttt
     5341 tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc tggttaaagc
     5401 cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact
     5461 gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca
     5521 gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg ctgcggcgcg
     5581 cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg cattggatat
     5641 tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat taagctacaa
     5701 agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg aactggataa
     5761 gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc gtaaccgctg
     5821 gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa atatcctgat
     5881 gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta agctggcgaa
     5941 tgcgccgttc atcaaagttg aagcgaccaa attcaccgaa gtgggctatg tcggtaagga
     6001 agtggattct attattcgcg atctgaccga tgccgccgtg aaaatggtac gcgtccaggc
     6061 tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc tcgacgtgct
     6121 gatcccacct gctaaaaaca actggggaca gaccgaacag cagcaggaac cgtccgctgc
     6181 tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag aaatcgagat
     6241 cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccaggca tggaagagat
     6301 gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa
     6361 gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga aactggtgaa
     6421 cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga tcgtgtttat
     6481 cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga
     6541 aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca ccaaacacgg
     6601 gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc
     6661 gtctgaccta atcccggaac tgcaaggtcg tctgccaatc cgcgttgaac tgcaggcact
     6721 gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca ccgtgcagta
     6781 caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg gtattaaacg
     6841 catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt
     6901 acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg atttaagcgg
     6961 tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt tggtggcaga
     7021 tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca ttgtttgatg
     7081 gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa ttagccgaac
     7141 tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg ctgcaattat
     7201 cgtcgggacg gcgctggcat ggtggcaagg tcattttgat ccgctggtcg ccctgctggc
     7261 actgattacc gccgggctat tacagatcct ttctaacctc gccaatgatt acggcgatgc
     7321 ggtaaaaggc agcgatagac ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt
     7381 cattacccag caagagatga aacgggcgct cattattacc gtcgtgctca tctgtctctc
     7441 cgggctggca ctggttgcag tggcgtgcca tacgctggct gattttgtcg gtttcctgat
     7501 tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc gtccttatgg
     7561 ttatatcggt ctgggggata tttccgtact ggttttcttt ggctggttaa gcgtcatggg
     7621 gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg cgaccgcatg
     7681 cggcctgctg gcaacggcag tactgaacat taataacctg cgtgatatca atagcgaccg
     7741 agaaaacggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca
     7801 tgcctgcctg ctgatgggct ctctggtatg tctggcgctg tttaatctct tttcgctgca
     7861 tagcctgtgg ggctggctgt tcctgctggc ggcaccgttg ctggtgaagc aagcccgtta
     7921 tgtgatgcgg gaaatggacc cggtggcgat gcgaccgatg ctggaacgca ctgtcaaggg
     7981 ggcgttactg actaacctgc tgtttatttt agggatattc ctaagccagt gggcagcata
     8041 actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag cgcgatatac
     8101 tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc cgagctttgt
     8161 gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt tggcggacgg
     8221 gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg gttgctgtac
     8281 gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt
     8341 cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga atgggaaggt
     8401 ctggttattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga tatcggcatc
     8461 caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga aagcgatgtc
     8521 cgcgtcaatt ttggcggtgt aaccttcttc tccggcgacc atctttatgc cgacaatacc
     8581 gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca ccgcaaggtg
     8641 ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa agatgaagcg
     8701 taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc agccgttctg
     8761 ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct gggcattttg
     8821 aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca tctgcaacag
     8881 agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca cttctaatga
     8941 cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct cgtcccgatt
     9001 accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt atgaagccgc
     9061 gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt tcatccttct
     9121 cctggtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta
     9181 acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct cattttatga
     9241 cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg ataagttaac
     9301 agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa acattaactc
     9361 ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc
     9421 tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg
     9481 gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg gcaatggcta
     9541 tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc attgcattgt
     9601 ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca caagttgccg
     9661 gcgctttctg tgctgcggct ttagtttacg ggctttacta caatttattt ttcgacttcg
     9721 agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggca ggcactttct
     9781 ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag atggtgatta
     9841 ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt gtaccacgcg
     9901 gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca tctatgggcc
     9961 cattgacggg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc
    10021 tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac ttcctggtgc
    10081 cgctttttgg ccctatcgtt ggcgcgattg taggtgcatt tgcctaccac aaactgattg
    10141 gtcgccattt gccttgcgat atctgtgttg tgaaagaaaa ggaaaccaca actccttcag
    10201 aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga aaaaaaatat
    10261 atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc
    10321 aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggttgggta
    10381 gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg
    10441 aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc
    10501 actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc
    10561 cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc
    10621 agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc
    10681 gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt tggtacggtt
    10741 gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga ttacaccaac
    10801 gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa
    10861 gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtatacggt
    10921 cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac
    10981 cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat
    11041 ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc
    11101 ctgctgacca ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg
    11161 gtgtttatgg caggcgcatc cattcagtgg ctgcgcgatg agatgaagtt gattaacgac
    11221 gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt
    11281 ccggcattta ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc
    11341 ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga gtctattgct
    11401 tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc
    11461 ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca gtccgatatt
    11521 ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg tgcggcctat
    11581 ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga gaaagcggtg
    11641 attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc
    11701 tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta atgtaaatgc
    11761 cggatgaagc gtttatgccg catccggtag tcctgaaacg tgcgggggca accccgcaca
    11821 catcaataat cccacccttc ccctgtgcta cacttcgcgc cattccttac tgcttagagt
    11881 ttgctatgag acgagaactt gccatcgaat tttcccgcgt caccgaatct gcggcgctgg
    11941 ctggctacaa atggttagga cgcggcgata aaaacaccgc ggacggcgcg gcggtaaacg
    12001 ccatgcgtat tatgctcaac caggtcaaca ttgacggcac catcgtcatt ggtgaaggtg
    12061 aaatcgacga agcaccgatg ctctacattg gtgaaaaagt cggtactggt cgcggcgacg
    12121 cggtagatat tgctgttgat ccgattgaag gcacgcgcat gacggcgatg ggccaggcta
    12181 acgcgctggc ggtgctggca gtaggcgata aaggctgctt cctcaatgcg ccggatatgt
    12241 atatggagaa gctgattgtc gggccgggag ccaaaggcac cattgatctg aacctgccgc
    12301 tggcggataa cctgcgcaat gtagcggcgg cgctcggcaa accgttgagc gaactgacgg
    12361 taacgattct agctaaacca cgccacgatg ccgttatcgc tgaaatgcag caactcggcg
    12421 tacgcgtatt tgctattccg gatggcgacg ttgcggcctc aattctcacc tgtatgccag
    12481 acagcgaagt tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgta gtttctgcgg
    12541 cggtgatccg cgcattagat ggcgacatga acggtcgtct gctggcgcgt catgacgtca
    12601 aaggcgacaa cgaagagaat cgtcgcattg gcgagcagga gctggcacgc tgcaaagcga
    12661 tgggcatcga agccggtaaa gtattgcgcc tgggcgatat ggcgcgcagc gataacgtca
    12721 tcttctctgc caccggtatt accaaaggcg atctgctgga aggcattagc cgcaaaggca
    12781 atatcgcgac taccgaaacg ctgctgatcc gcggcaagtc acgcaccatt cgccgcattc
    12841 agtccatcca ctatctggat cgcaaagacc cggaaatgca ggtgcacatc ctctgattga
    12901 tttgatcgat tgagccttcc agtccttcgg gactggaatt tttttgttcg gagaacgaag
    12961 ataaggcaag tcaatcaaaa caggagaaaa acatggctga ttgggtaaca ggcaaagtca
    13021 ctaaagtgca gaactggacc gacgccctgt ttagtctcac cgttcacgcc cccgtgcttc
    13081 cgtttaccgc cgggcaattc accaagcttg gccttgaaat cgacggcgaa cgcgtccagc
    13141 gcgcctactc ctatgttaac tcgcccgata atcccgatct ggagttttac ctggtcaccg
    13201 tccccgatgg caaattaagc ccacgattgg cggcactgaa accaggcgat gaagtgcagg
    13261 tggttagcga agcagctggc ttctttgttc tggatgaagt accagattgc gaaacgctat
    13321 ggatgctggc aaccggtaca gcgattggcc cttatttatc gattctgcaa ctaggcaaag
    13381 atttagatcg cttcaaaaat ctggtcctgg ttcacgccgc acgttatgcc gccgacttaa
    13441 gctatttgcc actgatgcaa gaactggaaa aacgctacga aggcaaactg cgcattcaga
    13501 cggtggtcag tcgggaaacg gcagcggggt cgctcaccgg acggataccg gcattaattg
    13561 aaagtgggga actggaaagc gcgattggcc tgccgatgaa taaagaaacc agccatgtga
    13621 tgctgtgcgg caatccacag atggtgcgcg atacacaaca gttgctgaaa gagacccggc
    13681 agatgacgaa acatttacgt cgccgaccgg gccatatgac agcggagcat tactggtaag
    13741 cggttactta tcgataaacg gcacgatgag caaatccgca ctcatcttat tgatcatccc
    13801 gcgatatgct ggcatcaaac ggttgataaa tgagtgatga tgaccacaga caaggaggtc
    13861 gcactgctct ttttgcataa tttccagcag tgtttccggc atttctccgc gttcaatacg
    13921 cagttttgtc ttcggccatt gaatattttt cgtcagttta tacagcttgt tatccgactt
    13981 attcttcaac aattgaagaa tatcttctgt tgcagggaag tagatacccg gatacaactc
    14041 gcttaagcca tcatcaatat gaattaacgt caggtgagcg tcattatgtc tggcgagctc
    14101 cagagcttta ttcaccagta aggcatcttc ttcattcccg gaaattgcca cgccaatgtg
    14161 tttataagcc atgtttaact ccttctaaag caactccatc aagctaacga acgcagtgat
    14221 aactcaaaaa taatcatctc ggcctggcat gagaaagtga aggccgcatc aagctcaact
    14281 tgcccctcca gatccttaat cttgtaggcc acgctggccc cttcatctgc agaggatgca
    14341 gcggcggcaa gctctttcag tttggtcata aaagactctg cttcttcgcg ggtggcaaaa
    14401 aagcgcgaaa acttactggt acagttgtcg ttatcaatca ccgtaccgat atcaatcgca
    14461 catcctttag tactgcattt atctacgaca tctttcatag ggcacctctg tatattcgcc
    14521 tctctgtcga ttacgactta agaataatgc gccgttaagg gaagaacaaa caagtaatga
    14581 tcttatggat taccattttt tttgatattg cttaaggtga agagttcaga aggattttcc
    14641 gggtattgtc atttgcatcg tggaaataac aatcccggaa aaaaatagca gtaagagata
    14701 tattaaaggt taattacttg atttattgtc ggctttatac ttcacatcct gagtatcttt
    14761 accatattta ttttcgcctt gtgtgccaac aaacgcacca agatcgataa gcatcatcac
    14821 cagaatcaac gtcgggacaa aacaccccac cgcccattgc cagacacccg gtaaaatcgc
    14881 ccagttaccc gccagcagca tccacgccac aatcatcaga aatgcccatg cgccggaacg
    14941 cccgcgatca tgcaagcgct taacagttac tgccgctgtt ggccaaagca agcacacaag
    15001 gcaaaacgcc gcggtctgaa tatcgagtaa atttttaccc gccagtgaga aaagcaccag
    15061 catgcctgcg aaccacaggc ctatccaaat ccagaaatca cggcgtccaa tacgcccttt
    15121 aaatgagaat aaccattgct gtatggtcat gtaagttcct tgatggttgt cttttccagg
    15181 attctaccct tttgacaagg gcgacagata tcgttttaat cggagccagt cataacaaaa
    15241 ggtactgtca atgaagccag ggtgtacgct gttttttctc ttatgttctg cattaaccgt
    15301 tacaacaacg gcgcatgcac aaacaccaga tacggcaacg accgcgcctt atctgctggc
    15361 tggagcccct actttcgatc tctccatcag ccagtttcga gaagacttta acagccagaa
    15421 tcctagcctg ccactgaacg aatttcgtgc catcgacagc agtcccgaca aagccaatct
    15481 cactcgtgct gcaagtaaaa ttaacgagaa cttgtatgct tctacagcgc tggagcgcgg
    15541 taccttaaaa atcaaaagca ttcaaatgac ctggctaccc atccaggggc cagagcaaaa
    15601 agcagcgaaa gcgaaagctc aggaatacat ggcagcagtg atccgcacac tcaccccatt
    15661 aatgaccaaa acacaaagcc agaaaaaact gcagtcgctg ctaacggcgg ggaaaaacaa
    15721 acgttattac accgagacag aaggtgcact gcgttatgtt gtcgcggaca acggcgaaaa
    15781 ggggctgacc ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc ttgaaggttt
    15841 gaataaatga caaaaagcaa agcctttgtg ccgatgaatc tctatactgt ttcacagacc
    15901 tgctgccctg cggggcggcc atcttccttt attcgcttat aagcgtggag aattaaaatg
    15961 cgacatcctt tagtgatggg taactggaaa ctgaacggca gccgccacat ggttcacgag
    16021 ctggtttcta acctgcgtaa agagctggca ggtgttgctg gctgtgcggt tgcaatcgca
    16081 ccaccggaaa tgtacatcga tatggcgaag cgcgaagctg aaggcagcca catcatgctg
    16141 ggtgcgcaaa acgtggacct gaacctgtcc ggcgcattca ccggtgaaac ctctgctgct
    16201 atgctgaaag acatcggcgc acagtacatc atcatcggcc actctgaacg tcgtacttac
    16261 cacaaagagt ctgacgaact gatcgcgaaa aaattcgcgg tgctgaaaga gcaggggctg
    16321 actccggttc tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa aactgaagaa
    16381 gtttgcgcac gtcagatcga cgcggtactg aaaactcagg gtgctgcggc attcgaaggt
    16441 gcggttatcg cttacgaacc tgtatgggca atcggtactg gcaaatctgc aactccggct
    16501 caggcacagg ctgttcacaa attcatccgt gaccacatcg ctaaagttga cgctaacatc
    16561 gctgaacaag tgatcattca gtacggcggc tctgtaaacg cgtctaacgc tgcagaactg
    16621 tttgctcagc cagacatcga cggcgcgctg gttggcggtg cgtctctgaa agctgacgct
    16681 ttcgcagtaa tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg acaggtgccg
    16741 gatttcatat ccggcactta ctttccttaa ctcttcgcct taacgcaaaa tctcacactg
    16801 atgatcctga atttcctcgg ctgaagcacg gttaagcgtc agtagatttc gttgtgtcgc
    16861 cagcaataca aatgagttat cactctgccg taccatcgcc agcccgtagc ttcccatatg
    16921 ttcccgcgcc tcaggtactt cttctgccag catcataaat gggctgcgtt gtaccagttc
    16981 gctttccgtt acccgacgcg ccaggtattc atgcccgcgc aaaccacctg gcagtggcaa
    17041 ccagcggctg ctgatgttcg ccagattgtt atccagttgt tcgcgcacat caggacgaat
    17101 acaagagata tgaatatgaa aatggttttg cgtacgcccg gtgcgggagt tgatcgccaa
    17161 agaaaccgcg cgatcgggaa ccggctggcc gtactttttg ctcataaaat cacgcgcctg
    17221 ccaggccaac caaaagaagt tcggcgttga aggatcggtc aacaaaggac tttcagtacc
    17281 gttaatacga tacgttggca tcaacagata ttgcagtggg ccattaagat cttttaaaac
    17341 cacgtatccg gcattgggtt tgacttccgc acatggcgaa ggattttgat tttgctgctg
    17401 attgggcaaa cattcctcaa ggacaatctt acgtaatgta tccgactctt caccggttaa
    17461 tttccagtaa ccaataccgg cagccacaac ggcgataact atcatcacca aaaaaagaag
    17521 acccgctttt ttcatctttt tttccctgta cctcaaagag atgcaagggt aacgcaaaat
    17581 cgtgacaaat aaaaaacccg gtcgcgagaa accaccgggt ttgataatta tcctggggaa
    17641 atcagcgttt gctgatctga tcgaacgtac cgccgttagc aaaatgctct ttttgcgctt
    17701 tcgtccagcc gccgaactct tcatcaatgg tgaataactt cagctttgga aacgcatttt
    17761 cgtacttttt cgctacttca gcgtcgcgcg gacggtagta gtttttcgcg gcaatttcct
    17821 gaccttctgg cgagtagaga tatttcaggt aggcttccgc cacctctttg gtgccttttt
    17881 tctcgaccac tttatcgacc accgacacgg ttggctctgc gaggatagac tcactcggcg
    17941 tgacgatttc gaatttatct ttccccagtt cattcgctgc cagcagagct tcgttttccc
    18001 aggcgatcag tacatcgcca atcccgcgct cgacaaaggt gttagtggag ccgcgcgcgc
    18061 cagaatccag aacttcgacg tttttataca gtgcccgaac aaaatcctgt gcttttgcct
    18121 gatcgttgtt gttgtgatgc agcgcgtagc cccaggcagc caggtagttc cagcgtgcgc
    18181 caccagagct tttcggatta ggcgtgatca ccgaaacacc cggtttaatc agatcgttcc
    18241 agtcatggat ctgcttcgga ttgcccttac gcaccaggaa aacaatggtg gaagtgtacg
    18301 gtgcggagtt atccggcaga cgtttgatcc actctttatc aatccgcccg cgttccgcaa
    18361 ttgcgtccac gtcataggcc atagccagcg tgacaacatc agcttcaata ccgttgatta
    18421 ccgacgtcgc ttgtttacct gaaccaccgt gtgactgacg catcaccacg ttatcaccag
    18481 tttgctgttt ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat tcgcgcgttg
    18541 gatcatatga aacgttaaga agctgaatat cctttgccat aacgctggtt gccgccagca
    18601 aaaatgttaa ccctacgccc cacttgttca tcgcccgact ctcttatgtt gtgttgtgat
    18661 gagcaaagcg tgccagaagg ttaaccaaac attaaagaat aaaaaaagat tggctataac
    18721 ttgcgggtat atgttgaggg attaaaaagg cggaagaaat ccgcctcata ttgctgacaa
    18781 agtgcgcttt gtccatgccg gatgcggcgc gaacgcctta tccggcctac aaaagtttgc
    18841 aaattcaata aattgcagaa ttcatgtagg cctgataagc gaagcgcatc aggcattttt
    18901 gcttctgtca tcggtttcag gctaaaggaa tctgcctttt tccgaaatca ttaatacagt
    18961 tttttcgcgc agtccagcca gtcacctttg aacggacgct tcatgttttc gatagcgtcg
    19021 atgatgtcgt ggtgaaccag ctgttcgttc tggataccta cacaacgacc gccgtaacct
    19081 gccagcagca gatcgatagc gtaagcgccc atacgggaag ccagaatacg gtcgtaaggc
    19141 accggagaac caccgcgctg gatgtggccc agcacagttg cgcgggtttc acgaccggtt
    19201 tctttctcga tgaaatgcgc cagttcgtca acatcacaca tatgttcggt aatcgccacg
    19261 atcgcgtgtt ttttaccttt cgcgataccc gctttgattt cgtttaccag gtcttcacgg
    19321 ctgaattcaa cttccggaac cacaacgaat tcacagccac cggcaatggc cgcagccaac
    19381 gtcagatcgc cacaataacg gcccatcact tccaccacgg aaatacgctg gtgagaagaa
    19441 gaggtgtcac gcagacggtc gatcgcttct acaacggtgc tcagcgcagt gaagaaaccg
    19501 atagtgtagt cagtgccttt gatgtcgttg tcgatagtgc ccggcagacc gatgcacggg
    19561 aagcccattt cggtcagacg cattgcaccc atgtaggaac cgtcaccgcc gataaccacc
    19621 agcgcgtcga taccacgttt tttcaggttt tcgatagcca cggcgcggat gttctcgtcg
    19681 cggaattccg ggaaacgcgc agaaccgagg aacgtaccgc cacggttgat catgtcagac
    19741 acgctgtaac ggtctagctg taccatacgg tcttcataca gacccagata gccgtcataa
    19801 atacccatta cttccagacc ttctgtcagc gcagaacgaa caaccccgcg aattgcggcg
    19861 ttcatgcctg gcgcatcacc gccgcttgtc aacacaccga ttttcttaat catgactacc
    19921 tctgaacttt ggaatgcaaa atgaaatctg ttgccggaag tcttcttgca catcgaagtg
    19981 atccaacgaa tgtgcaaata gtataacaat cacttcctgc tgaattgatt caggtcaggc
    20041 caaatggcgg tattttatac acaaaatgcg ggtctggctc tcttttatac tgattatgaa
    20101 agcatagacc gtttaccctc cctgggtacg acggaacagg ggtcctgatg gataattaca
    20161 tccgatcccg gaaaacgccg taaaatagcc tgctctacct gatccgccac catatgtgcc
    20221 tgaaccaaag gcagagagtc ttccatttcc aaatgaatct gaataaagcg ggtcggccct
    20281 gactgccgcg tgcgaagatc gtgagcgccg ctaacacccg gccaggaagt cacgatatca
    20341 ataatttctt gccgttcctc atcaggcaat gcgcgatcca gtaatgactg taccgcctca
    20401 tatcccatgc gtaacgcgct atataaaata tagatgccga ttcccaatgc aaacagagca
    20461 tcggcgcgat gccagccgta ccaggacaac cccagcgcca gcagaattgc gccgttcatc
    20521 ataacatcag actggtaatg tagcatatca gcccgcaccg cctggctttg cgtccggcgc
    20581 accacccaac gctgaaacga gacaaggata atcgtacaaa ttagcgccac aattgtcacg
    20641 ataaccccga cgcctggatc tatcatcggt gttggagata tcagatgttg aatacccgtc
    20701 aaaaacagga atagtgccga accggagata aacatacttt gcgccagcgc cgcgagggac
    20761 tctgctttac cgtgaccaaa cgagtgatta tcgtcggcag gttgcaggga atatcgcacc
    20821 accagtaaat tcgtcaacga cgcgccgata tccaccagcg aatccaccag cgcggcgaga
    20881 atactcaccg acccggtata ccaccatgca aaaattttaa tcagcaatag caacgaagcc
    20941 atcgccgtcg cagcaatcgc cgcccgactg accagccgtc cataagattg attcataaat
    21001 actcccgcta tcaactgacg ctagtataac ggaagcaaat catctgcaat gcattaagca
    21061 gcaggcaaat tgaggataaa aaaaacccca catcatgtgg gggaagacag ggatggtgtc
    21121 tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact caatagcttc
    21181 aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg ttgttgatgt
    21241 ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg gttgcggact
    21301 tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc ctgtgcgcgc
    21361 acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc cagttcgcta
    21421 acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat ctgctgacgc
    21481 tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg ctgcgtactg
    21541 cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg aaccgacttc agcagcgtgg
    21601 cttaatgaac tgactgccag cgttgaagcc atgacggcag cggtaactat gcgcatcatt
    21661 tgctcccaaa atctttctgt cgcgattcaa cgatagagag tttacgattc aggctgcaaa
    21721 catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa
    21781 tttctgcctc ggaggtattt aaacaatgaa taaaatcctg ttagttgatg atgaccgaga
    21841 gctgacttcc ctattaaagg agctgctcga gatggaaggc ttcaacgtga ttgttgccca
    21901 cgatggggaa caggcgcttg atcttctgga cgacagcatt gatttacttt tgcttgacgt
    21961 aatgatgccg aagaaaaatg gtatcgacac attaaaagca cttcgccaga cacaccagac
    22021 gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg gccttgagct
    22081 gggcgcagat gactatctcc cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg
    22141 cgcgatcctg cgccgttcgc actggagcga gcaacagcaa aacaacgaca acggttcacc
    22201 gacactggaa gttgatgcct tagtgctgaa tccaggccgt caggaagcca gcttcgacgg
    22261 gcaaacgctg gagttaaccg gtactgagtt taccctgctc tatttgctgg cacagcatct
    22321 gggtcaggtg gtttcccgtg aacatttaag ccaggaagtg ttgggcaaac gcctgacgcc
    22381 tttcgaccgc gctattgata tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa
    22441 agatggtcac ccgtggttta aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc
    22501 atgataggca gcttaaccgc gcgcatcttc gccatcttct ggctgacgct ggcgctggtg
    22561 ttgatgttgg ttttgatgtt acccaagctc gattcacgcc agatgaccga gcttctggat
    22621 agcgaacagc gtcaggggct gatgattgag cagcatgtcg aagcggagct ggcgaacgat
    22681 ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg cgattgataa gtgggcaccg
    22741 ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc
    22801 gaaatgcaga tcattcgtaa ctttattggt caggccgata acgccgatca tccgcagaag
    22861 aaaaagtatg gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg cgaagataat
    22921 taccaacttt atctgattcg tccggccagc agttctcaat ccgatttcat taacttactg
    22981 tttgaccgcc cgctattact gctgattgtc accatgttgg tcagtacgcc gctgctgttg
    23041 tggttggcct ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt
    23101 gcccagggaa acttacgcca gcacccggaa ctggaagcgg ggccacagga attccttgcc
    23161 gcaggtgcca gttttaacca gatggtcacc gcgctggagc gcatgatgac ctctcagcag
    23221 cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt
    23281 acggcattac tgcgccgtcg tagcggtgaa agcaaggaac tggagcgtat tgaaaccgaa
    23341 gcgcaacgtc tggacagcat gatcaacgat ctgttggtga tgtcacgtaa tcagcaaaaa
    23401 aacgcgctgg ttagcgaaac catcaaagcc aaccagttgt ggagtgaagt gctggataac
    23461 gcggcgttcg aagccgagca aatgggcaag tcgttgacag ttaacttccc gcctgggccg
    23521 tggccgctgt acggcaatcc gaacgccctg gaaagtgcgc tggaaaacat tgttcgtaat
    23581 gctctgcgtt attcccatac gaagattgaa gtgggctttg cggtagataa agacggtatc
    23641 accattacgg tggacgacga tggtcctggc gttagcccgg aagatcgcga acagattttc
    23701 cgtccgttct atcggaccga tgaagcacgc gatcgtgaat ctggcggtac aggattgggg
    23761 ctggcgattg ttgaaaccgc cattcagcag catcgtggct gggtgaaggc agaagacagc
    23821 ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt ataagcggag ttaaactccg
    23881 catttgtagg caggataagg cgtttacgcc gcatccggca tttgagcagg atgcctgatg
    23941 cgacgctgat agcgtcttat caggcctaca ctccgggggt tattttcccc acaaccggcg
    24001 ggaaaaatct tcgatcttgc cgctcagtcg gcgcttttgc atcgtccgcg tccagctttt
    24061 cgataacccg gcggcggaga gtaaacggtg atactgatcg tcatcaaacg gcatatgcca
    24121 tgcgatggcg gcagcctcct gcacggtcac atcgctgaca cgggaaacca actccagcgg
    24181 cgcgtccgca gacactttcc ctggcgctat cacgctatac aaccacccca ccttacctgt
    24241 gttttgcatc aactgcgcta tatcgctgat atcaaaatgg taattgagtt tatagcaagg
    24301 cgagcgcggc tggctgacct gaattaatgc ctctccccag cggaaaatat cgcccatata
    24361 aacattactt tccgtcaggc cgtcggttga gaggttttca ccaaacgcag gcgcaacaaa
    24421 caactccgcc tgttccggaa attcccgcgc ccagtagaga taatgctcac gaggataatg
    24481 acacagcgct ctgtctggcc caccatgaac ctttttctcc gcctgctcgt caccttccag
    24541 ccccagctcc gtcagcatca actcaccatc aacctggatt ttggcaattg cgctgggttt
    24601 gccttcggga taagcctgaa tcttgcctgt gtatacatca accggatatc gcatctgttc
    24661 cctctccacg agaaatttcc ccattagacc atatttattt tatgtcaggc gtgctgtttt
    24721 cgcggtaagt atccaggcag gaactcaata gcaaaccgga cgaacgttag ttcatccggc
    24781 tgtggggatt aaggaagcag gtttatttca ccgtacctaa aatttcgatt ttcgctgctc
    24841 tggctttgac tttacgtgac cagatagagg taagaattgg caccagaatc gaagtcacaa
    24901 tgaccgccgt cgctaccagc gaagttgctg ccggagccat cggtttaaac gcaggcacca
    24961 tttctgcaat cagcataggt gtcgctaccg cggcccctgc ggaactggaa gcggcaattc
    25021 cggcagtgcc atcgccaccg ccaatcaatt tatcggcgat aatcaacgga ataccggtca
    25081 cgataattac tgccacaccc aacaggatcc ccagcaaacc agtctgggca atcacagtca
    25141 aatcaatggt attgcccagc gcgaaggcaa agaatggaat cagcgtttgc accgctttgc
    25201 tgaaaaattc tcgtaattca gggtcaaggt tccccagggc aaagcccacc aggaacggta
    25261 atactgcgcc gacgaaaaca tgcggttcaa acgaggcaat ccctgcagtg cccagaataa
    25321 tcatcgtcat gagcggcccg gactccaacg acatcaacac aaatgcccca gcttcttctt
    25381 ttgtgccgta ctgctgcatg atggaagcgt aaagtccgcc attggtcata tccatcgccg
    25441 ccaccagcgc cagcgttgaa agtccggcaa agaatccaac ttcaacacca tgttccggaa
    25501 taatgcgcga ggcaattgcc gcaaccaccc acgcgacggc aatttttgtt accaccagcg
    25561 taccggattt acgcaatacc gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa
    25621 aaaaccacac cgccagaatg gacaccgtac cggtaatcat cccgttggtg aatgatccaa
    25681 aatatttccc cgcccccggc gagaaggtgt ggcacagtgc gccaaggaat agcgggacga
    25741 gcatcatccc cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct
    25801 gtcggcatat ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa
    25861 acgaagatag acattcattt aatcaataaa agtgatcaaa accacatcaa ttgaaacgct
    25921 gttttatttt tatcggatca ttgtttttat tttgaaattg atcacaaaaa aaccaccgcc
    25981 gttggcgatg gttcattttt taagctgata tgcggcgtaa acgcctcatt gcagcaggcg
    26041 gcaaatgatt attttttcgc cgcgaaacgt gccgctgctt catcccagtt caccacgttc
    26101 cagaactctt tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc
    26161 cacacatcca ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc
    26221 ggggaatcct ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat
    26281 gcccagccgg aaccaaagcg ggaagctgcc gctttctcaa attctgcttt gaagttatca
    26341 acggagccga agtcacgttc gatagccgct ttcaggtcac cctgtaaggt ggtgcctttt
    26401 ttcagacctt tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg
    26461 gttttcttgt ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg
    26521 gcaaattctg gcaggctttc cagcgccgcg ttggcgttgt tgacgtaggt ctgatggtgt
    26581 ttggtgtggt ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca
    26641 tacggcaggg atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt
    26701 gttaatgcgc gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat
    26761 caatgccgta cttttcgtaa gggtatggtt ttgcaggaaa attcccgaga tgtgaagcaa
    26821 atcacccact taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt
    26881 taatcgacag cacgattttt acactcatct cgtcggagat gtgacgcgac gaaaaatgat
    26941 gaggataaga agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg
    27001 gccagtgcag cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc
    27061 atgtggtcag ttggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg
    27121 ttactgccga atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt
    27181 tttctgttcg gcgctatgtg ggggatcggt aatatcaact acggcctgac catgcgttat
    27241 ctcggcatgt cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg
    27301 atgacgccaa ttatcaacgg caatttcgat gttctgctta ataccgaagg cggacgcatg
    27361 acgttgctcg gcgttctggt ggcgctgatt ggtgtaggga ttgtgactcg cgccgggcag
    27421 ttgaaagagc gcaaaatggg cattaaagcc gaagagttca acctgaaaaa agggctggtg
    27481 ctggcggtga tgtgcggcat tttctctgcc ggtatgtctt ttgcgatgaa cgccgcaaaa
    27541 ccgatgcatg aagccgctgc cgcactgggc gtcgatccac tgtatgtcgc tctgccaagc
    27601 tatgtcatca tcatgggcgg cggcgcgatc atcaacctcg gtttctgctt cattcgtctg
    27661 gcaaaagtga aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt
    27721 cacaatgtgt tactctcagc actgggcggg ttgatgtggt atctgcaatt ctttttctat
    27781 gcctggggcc acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg
    27841 agtttctatg tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaatgca
    27901 ggtcgccgtc cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgccaac
    27961 atcgtcggca tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg
    28021 ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca
    28081 cattcggtcg aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga
    28141 cacactctga cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc
    28201 tgacgcaaaa cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga
    28261 cttttcaggc tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt
    28321 ggcggcaacg aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac
    28381 agcaactcag ccatttcgtt agcaaacgac acatgctgac tactttcatg ctcaagctga
    28441 ccgataacct gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct
    28501 gcgctggcgc taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc
    28561 gggcaataaa taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg
    28621 tcagcatgaa tgtaaaagag atcgccacgg gtaatgcgat aagggcgatc gttgagtaca
    28681 tgcagaccat taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca
    28741 aaaacatctt gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa
    28801 tcatctttga gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt
    28861 tgattattcg caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc
    28921 atcccgtccc tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga
    28981 aaagtggtta ctgtcgctga atccgcagcg ataggcgatg tcagtaacgc tggcctcgct
    29041 gtgacgtagc agatgtcggg ccttcatcag tcgcaggcgg ttcaggtaac gctgaggcgt
    29101 cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc
    29161 cgccacggca tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa
    29221 gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag
    29281 cagtaattgc ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc
    29341 ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg
    29401 ccagtgagac ggatactgcc catccagttc ttgtggcagc aactgattca gcccggcgag
    29461 aaactgaaat cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg
    29521 ttcatacaga tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata
    29581 gggctgccca ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa
    29641 atcatgatga tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc
    29701 gttaccagac ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg
    29761 tcaacacggc gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa
    29821 ccacgtaaaa aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc
    29881 atcacatcac cacaatgaag caaattgtga acatcatcac gttcatcttt ccctggttgc
    29941 caatggccca ttttcctgtc agtaacgaga aggtcacgaa ttcaggcgct ttttagactg
    30001 gtcgtaatga aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc
    30061 tcggcgcatc cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga
    30121 cgctgcgcga aatccatcgt tttaacaatg gcctgcatag ccagaacggt tatgtcacct
    30181 ggaatgtgga tagcctggaa agtgccattc gtcttggatt aaacaaggtg tgcgaggaag
    30241 ggattcgtat cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc
    30301 aacagggtca gcgtgtgggc ctgcccgttg cttatcgcga tagccgctcc aatggcctaa
    30361 tggcgcaggc acagcaacaa ctcggcaaac gcgatattta tcaacgtagc ggcatccagt
    30421 ttctgccctt caatacgctt tatcaactgc gtgcgctgac ggagcaacaa cctgaactta
    30481 ttccacacat tgctcacgct ctgctgatgc cggattactt cagctatcgc ctgaccggca
    30541 agatgaactg ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg
    30601 actgggacga gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga
    30661 cgcatccggg taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg
    30721 tcgccgttgc cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac
    30781 gcgctgctta tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat
    30841 ttaccaatga cacggcgctg gcagccaaca taaccaatga aggcggggcg gaaggtcgct
    30901 atcgggtgct gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggagcggc
    30961 aaatcaacga tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca
    31021 tcatcaatcc caatgacgat cgctttatta atcctgaggc gatgtgcagc gaaattcagg
    31081 ctgcgtgtcg ggaaacggcg caaccgatcc cggaaagtga tgctgaactg gcgcagctac
    31141 gcggtgaaga tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac acgctgctca
    31201 accagctatg tgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt gaagcctcga
    31261 cgctcggcaa tatcggcatc cagttaatga cgctggatga actcaacaat gtggatgatt
    31321 tccgtcaggt cgtcagcacc accgcgaatc tgaccacctt tacccctaat cctgacagtg
    31381 aaattgccca ctatgtggcg cagcttcact ctacacgaca gacaaaggag ctttgcgcat
    31441 gaccactcaa ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg cggtggggat
    31501 tgatgtcgag gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc actgctggca
    31561 gggcgatgat gtttccggtt ttgaaaaccc ggaaggttcg ctgaccgggg ggattcaggc
    31621 cacaggcaat tatccgggca aagcgcgtaa tgccagtgag ctacgtgccg atctggaaca
    31681 ggctatgcgg ctgattccgg ggccgaaacg gcttaattta catgccatct atctggaatc
    31741 agatacgcca gtctcgcgcg accagatcaa accagagcac ttcaaaaact ggggtgaatg
    31801 ggcgaaagcc aatcagctcg gtctggattt taacccctcc tgcttttcgc atccgctaag
    31861 cgccgatggc tttacgctct cgcatcccga cgacagcatt cgccagttct ggattgatca
    31921 ctgcaaagcc agccgtcgcg tttcggccta ttttggcgag caactcggca caccatcggt
    31981 gatgaacatc tggatcccgg atggtatgaa agatatcacc gttgaccgtc tcgccccgcg
    32041 tcagcgtctg ctggcagcac tggatgaggt gatcagcaag aagctaaacc ctgcgcacca
    32101 tatcgacgcc gttgagagca aattgtttgg cattggcgca gagagctaca cggttggctc
    32161 caatgagttt tacatggggt atgccaccag ccgccagact gcgctgtgcc tagacgccgg
    32221 gcacttccac ccgactgaag tgatttccga caagatttcc gccgccatgc tgtatgtgcc
    32281 gcagttgctg ctgcacgtca gccgtccggt tcgctgggac tgcgatcacg tagtgctgct
    32341 ggatgatgaa acccaggcaa ttgccagtga gattgtgcgt cacgatctgt ttgaccgggt
    32401 gcatatcggc cttgacttct tcgatgcctc tatcaaccgc attgccgcgt gggtcattgg
    32461 tacacgcaat atgaaaaaag ccctgctgcg tgcgttgctg gaacctaccg ctgagctgcg
    32521 caagctggaa gcggcgggcg attacactgc gcgtctggca ctgctggaag agcagaaatc
    32581 gttgccgtgg caggcggtct gggaaatgta ttgccaacgt cacgatacgc cagcaggtag
    32641 cgaatggctg gagagcgtgc gggcttatga aaaagaaatt ttgagccagc atgggtaaac
    32701 actgccggat gcggcgcgag cgccttatcc ggcctacggg tcggcaacag ttgtaggcct
    32761 gataagacgc gacagcgtcg catcaggcat tgattgccgg atgcggcaca agcgccttat
    32821 ccggcctacg ggtcgtgcat ctgacaacac cgaatttaca ggaacacaga acatgcaaaa
    32881 cattacccag tcctggtttg tccagggaat gatcaaagcc accaccgatg cctggctgaa
    32941 aggctgggac gaacgcaacg gcggcaacct gacgctgcgc ctggatgacg ccgatatcgc
    33001 gccttataaa gacaacgtcc atgcacaacc gcgctatatc ccgctcagcc agcccatgcc
    33061 tttactggca aatacaccgt ttattgtcac cggctccggc aaattcttcc gtaacgtcca
    33121 gctcgatcct gcagctaact taggcgtcgt aaaagtcgac agcgatggcg cgggctacca
    33181 tattctctgg gggttaacca acgaagccgt cccgacatcc gaactcccgg cgcactttct
    33241 ctcccactgc gagcgcatta aagccaccaa cggtaaagat cgggtgatca tgcactgcca
    33301 cgccaccaac ctgatcgccc tcacctatgt actggaaaac gacaccgcgg tctttactcg
    33361 ccaactgtgg gaaggcagca ccgagtgtct ggtggtattc ccggatggcg ttggcattct
    33421 gccgtggatg gtgcccggca cggacgaaat cggccaggcg accgcacaag agatgcaaaa
    33481 acattcgctg gtgttgtggc ccttccacgg cgtcttcggc agcgggtcga cgttggatga
    33541 aacattcggt ttaatcgaca ccgcagaaaa atcagcacaa gtattagtga aggtttattc
    33601 gatgggcggc atgaaacaga ccatcagccg tgaagagttg atagcgctcg gcaagcgctt
    33661 cggcgttaca ccactagcca gtgcgctggc gctgtaagga gcaaacatga tccgcaaagc
    33721 ctttgtcatg caggttaacc ccgacgccca cgaagagtat cagcgtcggc ataaccccat
    33781 ctggccggaa ctggaagcag tgttgaaatc tcacggtgcg cataactacg ccatttacct
    33841 cgacaaagcg cgtaatctgc tgtttgccac ggtagagatt gaatctgaag aacggtggaa
    33901 tgcggtagcc agtactgatg tttgccaacg ttggtggaaa tatatgaccg atgttatgcc
    33961 cgctaacccg gataacagcc cggtgagtag cgagctg
//
LOCUS       DADLTD010000018        33673 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLTD010000018 DADLTD010000000
VERSION     DADLTD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.018"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            55..1133
                     /gene="cas7e"
                     /locus_tag="JN701_000693"
                     /pseudo
     CDS             55..1133
                     /gene="cas7e"
                     /locus_tag="JN701_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            1144..1890
                     /gene="cas5e"
                     /locus_tag="JN701_000694"
     CDS             1144..1890
                     /gene="cas5e"
                     /locus_tag="JN701_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HAY7363227.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            1872..2522
                     /gene="cas6e"
                     /locus_tag="JN701_000695"
     CDS             1872..2522
                     /gene="cas6e"
                     /locus_tag="JN701_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HAY7363228.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            2519..3439
                     /gene="cas1e"
                     /locus_tag="JN701_000696"
     CDS             2519..3439
                     /gene="cas1e"
                     /locus_tag="JN701_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HAY7363229.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYAPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            3439..3732
                     /gene="cas2"
                     /locus_tag="JN701_000697"
     CDS             3439..3732
                     /gene="cas2"
                     /locus_tag="JN701_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HAY7363230.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(3939..4976)
                     /gene="iap"
                     /locus_tag="JN701_000698"
     CDS             complement(3939..4976)
                     /gene="iap"
                     /locus_tag="JN701_000698"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY7363231.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            5228..6136
                     /gene="cysD"
                     /locus_tag="JN701_000699"
     CDS             5228..6136
                     /gene="cysD"
                     /locus_tag="JN701_000699"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY7363232.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            6138..7565
                     /gene="cysN"
                     /locus_tag="JN701_000700"
     CDS             6138..7565
                     /gene="cysN"
                     /locus_tag="JN701_000700"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY7363233.1"
                     /translation="MNTALAQQIANEGSVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            7565..8170
                     /gene="cysC"
                     /locus_tag="JN701_000701"
     CDS             7565..8170
                     /gene="cysC"
                     /locus_tag="JN701_000701"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY7363234.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            8220..8543
                     /locus_tag="JN701_000702"
     CDS             8220..8543
                     /locus_tag="JN701_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY7363235.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            8737..9048
                     /gene="ftsB"
                     /locus_tag="JN701_000703"
     CDS             8737..9048
                     /gene="ftsB"
                     /locus_tag="JN701_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY7363236.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            9067..9777
                     /gene="ispD"
                     /locus_tag="JN701_000704"
     CDS             9067..9777
                     /gene="ispD"
                     /locus_tag="JN701_000704"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY7363237.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            9777..10256
                     /gene="ispF"
                     /locus_tag="JN701_000705"
     CDS             9777..10256
                     /gene="ispF"
                     /locus_tag="JN701_000705"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY7363238.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            10253..11302
                     /gene="truD"
                     /locus_tag="JN701_000706"
     CDS             10253..11302
                     /gene="truD"
                     /locus_tag="JN701_000706"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY7363239.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            11283..12044
                     /gene="surE"
                     /locus_tag="JN701_000707"
     CDS             11283..12044
                     /gene="surE"
                     /locus_tag="JN701_000707"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY7363240.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            12038..12664
                     /gene="pcm"
                     /locus_tag="JN701_000708"
     CDS             12038..12664
                     /gene="pcm"
                     /locus_tag="JN701_000708"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY7363241.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            12804..13895
                     /gene="nlpD"
                     /locus_tag="JN701_000709"
     CDS             12804..13895
                     /gene="nlpD"
                     /locus_tag="JN701_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY7363242.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPLQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            13958..14950
                     /gene="rpoS"
                     /locus_tag="JN701_000710"
     CDS             13958..14950
                     /gene="rpoS"
                     /locus_tag="JN701_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY7363243.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(15044..16408)
                     /locus_tag="JN701_000711"
     CDS             complement(15044..16408)
                     /locus_tag="JN701_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAY7363244.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(16497..17273)
                     /locus_tag="JN701_000712"
     CDS             complement(16497..17273)
                     /locus_tag="JN701_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAY7363245.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(17278..17916)
                     /locus_tag="JN701_000713"
     CDS             complement(17278..17916)
                     /locus_tag="JN701_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7363246.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(17913..19174)
                     /locus_tag="JN701_000714"
                     /pseudo
     CDS             complement(17913..19174)
                     /locus_tag="JN701_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(19171..20079)
                     /locus_tag="JN701_000715"
     CDS             complement(19171..20079)
                     /locus_tag="JN701_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7363247.1"
                     /translation="MKTGSEFHVGIVGLGAMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            20245..21042
                     /locus_tag="JN701_000716"
     CDS             20245..21042
                     /locus_tag="JN701_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7363248.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(21093..21749)
                     /gene="pphB"
                     /locus_tag="JN701_000717"
     CDS             complement(21093..21749)
                     /gene="pphB"
                     /locus_tag="JN701_000717"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY7363249.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(21855..24416)
                     /gene="mutS"
                     /locus_tag="JN701_000718"
     CDS             complement(21855..24416)
                     /gene="mutS"
                     /locus_tag="JN701_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY7363250.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            24492..24626
                     /locus_tag="JN701_000719"
     CDS             24492..24626
                     /locus_tag="JN701_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363251.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            24703..25056
                     /locus_tag="JN701_000720"
     CDS             24703..25056
                     /locus_tag="JN701_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY7363252.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(25093..27171)
                     /gene="flhA"
                     /locus_tag="JN701_000721"
     CDS             complement(25093..27171)
                     /gene="flhA"
                     /locus_tag="JN701_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY7363253.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVYSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(27245..28255)
                     /gene="hypE"
                     /locus_tag="JN701_000722"
     CDS             complement(27245..28255)
                     /gene="hypE"
                     /locus_tag="JN701_000722"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY7363254.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(28252..29373)
                     /gene="hypD"
                     /locus_tag="JN701_000723"
     CDS             complement(28252..29373)
                     /gene="hypD"
                     /locus_tag="JN701_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY7363255.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(29373..29645)
                     /gene="hypC"
                     /locus_tag="JN701_000724"
     CDS             complement(29373..29645)
                     /gene="hypC"
                     /locus_tag="JN701_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY7363256.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(29636..30508)
                     /gene="hypB"
                     /locus_tag="JN701_000725"
     CDS             complement(29636..30508)
                     /gene="hypB"
                     /locus_tag="JN701_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY7363257.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(30512..30862)
                     /gene="hypA"
                     /locus_tag="JN701_000726"
     CDS             complement(30512..30862)
                     /gene="hypA"
                     /locus_tag="JN701_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7363258.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            31074..31535
                     /gene="hycA"
                     /locus_tag="JN701_000727"
     CDS             31074..31535
                     /gene="hycA"
                     /locus_tag="JN701_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY7363259.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            31660..32271
                     /locus_tag="JN701_000728"
     CDS             31660..32271
                     /locus_tag="JN701_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7363260.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            32268..>33673
                     /gene="hycC"
                     /locus_tag="JN701_000729"
     CDS             32268..>33673
                     /gene="hycC"
                     /locus_tag="JN701_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY7363261.1"
                     /translation="MSAISLINSGVVWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAIC"
BASE COUNT         7952 a         8654 c         9069 g         7998 t
ORIGIN      
        1 atattaccag gctggcgacg ccgataacga acaaaattaa taaaggacta tctcatgaca
       61 acatttattc agcttcattt gttaaccgct taccctgctg ccaatcttaa ccgtgatgat
      121 tccggctctc cgaaaactgt gattctgggt ggagcaacgc gtctgcgcgt ttcctcgcaa
      181 agtctgaagc gtgcttggcg cacttctgaa ctttttgaac aggcgctggc gggccatatt
      241 ggtgttcgta gcgggcgtat tgcgcgtgag gcggcaacta tcctgattga gaaaggaatc
      301 gaagataaaa aagccatcga atgggcagta gagatagccg attatcttgg gaaagccaaa
      361 aaagacaaaa aacaaaaaaa cgacaaaaaa ccaaaagatc cgctcactag cgccgaaact
      421 gaacagctcg tccatatcag cccggcagaa tttgacgccg taaaagcgct ggcccatcaa
      481 ctggcagaag aaaagcgcgc gccaaaagag aaagatctcg ctttgttacg taaagaccgt
      541 atggcggtag atatcgctat gtttggtcgt atgctggcga aaaaacccgg ggttaatgta
      601 gaagccgcct gccaggtcgc gcatgcgttt ggtgtcagtg aaacgattgt cgaaaatgat
      661 tttttcaccg ccgtagatga tttacgccag gcttctgaag atgccggtgc cggtcatgta
      721 gatgaaaccg gattcggttc tgcgctgttc tacacctata tcttatctgc attgataaag
      781 atctgctggt agaaaacctc ggcggtgacg aagcgttagc taatcagacc atacgcgcct
      841 ttacggaagc cgcgcttaaa gtctccccaa ccggtaaaca aaacagcttt gccagccggg
      901 cttatgcctc ctgggcgctg gcagaaaaag gcaccgatca accgcgttct ctggcggcag
      961 ctttctatga acccattaat ggcacccgtc agttagaggt ggcggtgcag cgtattacaa
     1021 ctcttcgcga aaatatgaat acggtctacg aacagaagac cgattacgca agttttgacg
     1081 tgatgaacaa acagggaagc atgaaggacg tgctggactt tatctgcgcg taaggggcgg
     1141 cttatgagcc aatatctgat ttttcagctt catgggccga tggcatcctg gggcgtcgat
     1201 gcccccggcg aagtgcgtca tacccatgaa ctgccttcgc tctcagcatt gctggggctg
     1261 ctggctgccg gggtagggat ccggcgtgat gataccgaac gcttaaacgc gtttaatcgc
     1321 cactattcac tggtggtttg cgccagccgt aacccgcgct gggcacggga ttatcacacg
     1381 gtccagatgc caaaagaggt gcgtaaagca cgttatttca gccgtcgcga agagttgagc
     1441 gaccctgagc ttttgagcgc gattatctcc cggcgcgact actacaccga tgcctggtgg
     1501 atggtggcgg tggcaacaac agccgatgcg ccttacagcc tcgaacagtt gcaggacggt
     1561 ttacgccatc cagtttttcc gctttatctg gggcgaaaaa gtcatccgct ggcattacct
     1621 cttgcgccgt tactgctcga aggcaacgcg tgtgatgcct tatgtaatgc ataccaacag
     1681 tatcaggatc atttccataa actgaaagtt tcactcccga aacttcagga tgaatgctgg
     1741 tgggaaggga aacacgacgg cctggtcgcg agcaaaatat tacgccgccg ggatgttcct
     1801 ttaaatcgtc agcagtggct gtttggggaa cgcaccgtca atcaggggcc gtggctcagc
     1861 aaggaggaac catgtacctc tcaagaataa ccttacatac cggccagctt tcgcctgcgc
     1921 agttgctgca tctggtggat cgcggtgaat atgtgatgca tcagtggctg tgggatctct
     1981 tccctagcgg caaagaaagg caatttcttt atcgtcggga agaactccag ggcgcgtttc
     2041 gcttttttgt actgtcgcag gaacgcccgg cggaaagtga tacgttcacc atagaatgcc
     2101 ggtcatttgc gcccgagctg tgtaccggac aacaactttg tttcaacctg cgggcgaatc
     2161 caacaatctg taaagcgggc aagcgccacg atctgctgat ggaggcgaaa cggcaggtga
     2221 gggggcaggc ggaaggaagt gatgtctggt tgcatcaaca acaggcggcg ctggactggc
     2281 tggcagcgca gggggagaga agtggtttta cactgctgga tatctcggtt gatgcctacc
     2341 gacaacagca actgcggcgg gaaaactccc ggcaactgat ccagttcagt agcgtggatt
     2401 atacggggat gcttacggtc accgatcccg ggttatttct gcaacgtctc agtcaggggt
     2461 atggcaaaag ccgcgcgttc ggctgtggtc tgatgctgat caaacccgga gcagaggcgt
     2521 gacgtttgta ccgctgagcc cgatcccgtt aaaagatcgc acctctatga tcttcctcca
     2581 gtacgggcaa atcgacgtac tggacggcgc tttcgtgctg atcgataaaa ccgggatccg
     2641 cacgcacatt ccggtgggat cggtcgcctg cattatgctc gaaccgggaa cgagagtttc
     2701 ccacgcggcg gtgcatctgg ccgccacggt ggggacgcta caggtctggg tcggtgaagc
     2761 gggtgttcgc gtctactctt ccggacaacc cggtggggcg cgtgctgata agttactcta
     2821 ccaggcaaag ctggctttaa cggaagatct acgtctgaag gtggtgcgca aaatgtatga
     2881 attacgtttt cgtgagccac cgccagctcg ccgttcagtg gagcagctac gtggaattga
     2941 gggatctcgc gttcgccaga cgtatgcatt actggcgaaa caatatggtg tgaaatggaa
     3001 tggtcgcaaa tacgctccta aagactggga aaaaggcgat gttgtgaatc gctgcatcag
     3061 tgctgctaca tcatgtctgt acggtatatc tgaagcggca gtattagccg cgggatatgc
     3121 gcccgctatt gggtttatcc atagtggcaa accgctttca tttgtttatg acatagccga
     3181 tatcattaaa tttgattcgg ttgtaccaaa ggcatttgaa atagcggcga ggcaacccgc
     3241 agaacctgat aaagaagtca gattagcctg tcgtgatatt ttccgtagca ctaagttaac
     3301 gggcaaatta ataccgttaa ttgaggaagt ccttgccgca ggtgaaattg aaccgccaca
     3361 acctgcgccg gatatgttac cgccagccat ccccgaacct gaaacgttgg gcgatagcgg
     3421 tcatcgagga cgcggctgat gagcatggtc gtggttgtta cagaaaatgt cccgccacgc
     3481 ttacgtggac ggctcgcaat ctggctactg gaagtgcgtg ccggtgtgta tgttggtgat
     3541 acatcaaaac gtattcggga gatgatctgg cagcaaatta cccaactggc tggttgcgga
     3601 aatgtggtga tggcctgggc gaccaatacc gagtcgggtt ttgaatttca gacctgggga
     3661 gaaaacagac gtattccggt ggatttggat gggttacgtt tggtttcttt tcttcctgtt
     3721 gataatcaat aagttatccg ttctttaaaa ataaggaaat gttttaattt agttggtaga
     3781 ttgttgatgc ggaataaatt tgtttaaaaa cagttatgta tgcttagtgt gttccccgcg
     3841 ccagcgggga taaaccgggc gcactggatg cgataatgga tatcacttag aattccccgc
     3901 ccctgcggta gaactcccag ctcccatttt caaacccatc aagacgcctt cgccagctcc
     3961 ttcaccaacg gtagcattat ccgcataacg tcacggcagc gacgttctat tcttccagga
     4021 agtgccttat caatatgctg ttgattatcc agtcttacgt catgccagct atttcccgca
     4081 gggaatgcgg ctgtttttgc gcgttgctga taaccatcct tattcccaag attccagtta
     4141 gtcgcttcca ccgaaagtac agcaatgccc gctttgtcga atatttctgc gtcattacaa
     4201 cacccagtgc ctttcggata atttttattc aaccccggat tggtcgttgc ggctattccg
     4261 tgactgcgcg caattgccag cgccctgtcg cgcgttaatt tccttactgc ttcaggggtt
     4321 tttacaccgc tgttgaaata caatttatcg ccaacaatta agttatcgag attaatcacc
     4381 agcagcgtat ttttcttttc ggtgtcactc atccgcttga gtaaattctc agcgcctaat
     4441 ttcccttcct cttcgccgct ggtcgccaca aatcgaatac catactcggt aggcgtattt
     4501 ttcaggcgtt ctgccaattc cagcatgaca cctaaacctg cggcgttatc atccattcct
     4561 tgtaacgtca gcccgccgag attggcatcg gcgtcagcat cgctcagcgg ggcgtaagta
     4621 tccagatgcg ccataatgat gatctgctgc ggcgctttgc cttcatgagc ggcaatcacc
     4681 gtacttcccg tcacgttatg ccagctctta cgattatcgc gggcggtata aatataccga
     4741 ctattaaatg tccgaatatc actgcgataa cccatttgct gaaactgttg gcgaatataa
     4801 tcggcagata acatttctgc aggagttccg gtcatgcgtc ccggaaagaa agtagcaata
     4861 tgtcgtgcct gagtattagc aaaatcgcca ggtttaggtg acgaggcgtg tacggggaga
     4921 ataaagcata cgccgagcgc cagggcagcg gtacggtggc gcaatgcgga aaacatagtg
     4981 agtccttaaa taccatgcaa atttttttac cgccatagta tgaaactgcc gctgcgctaa
     5041 aacaatttca aatcttccta aacgcccgaa atccggtgcc ttaagcactt tttgatatta
     5101 gctttgccaa atcgttattc cgttaaggaa ctactcattc taattggtaa tttcattcgt
     5161 tctcttacgc tccctatagt cgaaacatct gatggcaaga aaatagcggt attgcaaagg
     5221 aacggttatg gctcaaaaac gacttactca cctgcggcaa ctggaggcgg aaagcatcca
     5281 cattattcgc gaggtggcgg cagaattctc aaatccggtg atgctctact ctatcggtaa
     5341 agattccagc gtcatgctgc atctggcgcg caaggcgttt tatccaggta cgctgccttt
     5401 cccgttgctg catgtcgata ccggctggaa attccgcgag atgtatgagt tccgcgatcg
     5461 tactgctaaa gcctacggct gcgagctgct ggtgcataaa aacccggaag gcgtggcgat
     5521 ggggattaat ccattcgtac acggcagcgc gaaacatacc gatattatga aaactgaagg
     5581 cctgaaacag gcgctgaaca aatatggttt tgatgccgcc ttcggtggtg cgcgccgtga
     5641 tgaagagaaa tcccgcgcta aagagcgaat ctactctttc cgtgaccgct tccatcgctg
     5701 ggatccgaaa aatcagcgcc cggagctgtg gcacaactac aacgggcaaa ttaacaaagg
     5761 tgaaagcatc cgcgtcttcc cgctctctaa ctggaccgag caggatatct ggcaatacat
     5821 ctggctggaa aatatcgaca ttgtgccact gtatctcgct gcggaacgtc cggttctgga
     5881 acgcgacggt atgttgatga tgattgatga caaccgtatc gacctgcaac cgggcgaagt
     5941 gattaaaaaa cggatggtgc gtttccgtac gctgggctgc tggccgctga ccggtgcggt
     6001 ggagtcaaat gcacaaacac tgccggagat catcgaagag atgctggttt ctaccaccag
     6061 tgaacgtcag ggccgcgtga ttgaccgcga ccaggcgggg tctatggagc tgaaaaaacg
     6121 tcaggggtat ttttaagatg aacaccgcac ttgcacaaca aatcgccaat gaaggcagcg
     6181 tcgaagcctg gatgattgcg caacaacata aaagcctgct gcgttttctg acctgtggta
     6241 gcgtcgatga cggcaaaagt accctgattg gtcgcctgct gcacgatacc cgccaaatct
     6301 acgaagatca gctctcatcg ctgcataacg acagtaagcg tcacggcacc cagggcgaaa
     6361 agctggatct ggcactgctg gtggacggcc tgcaagctga gcgcgaacag ggcatcacca
     6421 ttgacgtggc ttaccgttat ttctctaccg agaagcgtaa atttattatc gccgacaccc
     6481 cagggcacga gcagtacact cgcaatatgg caactggcgc atcgacatgt gaactggcga
     6541 tcttactgat cgatgcccgt aaaggcgtgc tcgatcaaac ccgtcgtcac agttttatct
     6601 ccacactgtt ggggatcaaa catctggtcg tggcgatcaa caaaatggat ctggtggatt
     6661 acagtgaaga gacgttcacc catattcgtg aagattattt gacctttgcc gggcagctgc
     6721 cgggtaatct ggatatccgc tttgtgccgc tctctgcact ggaaggcgac aacgtggcat
     6781 cgcaaagtga aagtatgtcg tggtacagcg gtccgacact gctcgaagtg ctggaaaccg
     6841 tggagatcca gcgagtggtg gatgcccagc caatgcgctt cccggtgcag tacgttaatc
     6901 gcccgaatct cgattttcgt ggctacgccg gaacgctggc atccggtcgc gtggaagtcg
     6961 ggcaacgtgt caaagcgctg ccctctggtg tggaatcaaa cgtcgcgcgg atcgtgactt
     7021 ttgatggtga tcgcgaagaa gcctttgccg gagaagcgat caccctggtg ctgacggatg
     7081 agatcgacat cagccgtggc gatctgctgc tggcggcaga cgaagcgtta ccggcggtgc
     7141 cgagcgcgtc ggtggatgtg gtatggatgg cggaacagcc gctttccccg ggccagagtt
     7201 acgacatcaa aattgccggt aagaagacgc gcgcgcgtgt tgatggcatt cgctatccgg
     7261 ttgatattaa taaccttacc cagcgtgaag ttgaaaacct gccactgaat gggatcggcc
     7321 tcgtggatct cacttttgac gagccgctgg tgttagatcg ttatcaacaa aatccggtga
     7381 cgggtgggct gatttttatc gatcgcctga gcaacgtgac tgttggtgcc ggtatggtgc
     7441 atgagccgaa aaacgaggcc gccgttgcgt catctgaatt cagtgcattc gaactggaat
     7501 tgaatgccct ggttcgtcgc cactttccgc actggggcgc gcgcgatttg ctgggggata
     7561 aataatggcg ctgcatgacg aaaacgtcgt ctggcatagc catccggtca ctgtgcaaca
     7621 acgcgagcta caccacggtc atcgtggtgt agtgctgtgg tttaccggcc tctctgggtc
     7681 cggtaaatca acggtcgccg gggcgctgga ggaggcgtta cataaactcg gcgtcagtac
     7741 gtatctgctg gatggcgaca atgttcgcca cggattatgc agcgatctcg gttttagcga
     7801 tgccgatcgt aaagagaata tccgtcgcgt cggtgaagtg gcgaatttga tggttgaagc
     7861 cggactggtg gtgctgaccg catttatctc gccacaccgc gccgaacgcc agatggttcg
     7921 cgaacgcgta ggagaagggc gctttatcga agtgtttgtc gatacgccgc tggcgatttg
     7981 cgaagcccgc gatcccaaag gcttatataa gaaagcgcgt gccggtgaac tgcgcaactt
     8041 tacgggaata gattccgttt acgaagcgcc tgaatcggca gaaattcatc tcaatggtga
     8101 acaattagta acaaatttgg tacagcaatt attagatctg ttgagacaga acgatattat
     8161 cagatcctga gacaccaccg ggtttccatg cccggttcgt caaagtaaca ggatcaaata
     8221 tgcgtaatag ccataacatt acactaacaa ataacgacag ccttactgag gatgaagaaa
     8281 ccacatggtc actacctggt gccgtggtcg gttttatctc ctggttattt gcgctggcga
     8341 tgccgatgtt gatttatggc tctaacacgc tgttcttctt tatctacacc tggcctttct
     8401 ttctggcact gatgcctgtc gcggtagtgg tggggattgc gctgcattca ttgatggacg
     8461 gaaagctacg ctacagtatt gttttcaccc tggtgacagt aggcattatg tttggcgcac
     8521 tgtttatgtg gctactgggc taatttgtac tttcttgtct cctctgttca taattcaaac
     8581 cgtaactaat aatgagatta tgttctgcac gccctaggta tacgtaacaa tggacaaatg
     8641 tggtacattt gcccgcgttg tcgcggtatc cccaacagag gatgtagagt cgtcttcgga
     8701 tgcatgggat gatgatgccg tttttcaggg ggcaggatgg gtaaactaac gctgctgttg
     8761 ctggctattc tggtctggct acagtattcg ctgtggttcg gtaagaacgg tatacatgac
     8821 tatacccgcg tcaatgatga tgtggcggca cagcaagcta caaacgcgaa acttaaagcg
     8881 cgaaacgatc aactttttgc cgaaattgac gatctcaatg gcggccagga ggcgctcgaa
     8941 gagcgtgcgc gtaatgaact cagcatgacc aggccgggcg aaacttttta tcgtctggtg
     9001 cctgacgcgt cgaagcgcgc acagtctgcg gggcaaaaca atcgataaat cagcccggga
     9061 attaacatgg caaccactca tttggatgtt tgcgccgtgg ttccggcggc cggatttggc
     9121 cgtcgaatgc aaacggaatg tcctaagcaa tatctctcaa tcggtaatca aaccattctt
     9181 gaacactcgg tgcatgcgct gctggcgcat ccccgggtga aacgtgtcgt cattgccata
     9241 agtcctggcg atagccgttt tgcacaactt cctctggcga atcatccgca aatcaccgtt
     9301 gtagatggcg gtgatgagcg tgccgattcc gtgctggcag gtttgaaagc cgctggcgac
     9361 gcgcagtggg tattggtgca tgacgccgct cgtccttgtc tgcatcagga tgacctcgcg
     9421 cgattgttgg cgttgagcga aaccagccgc acgggaggga tcctagccgc accagtgcgc
     9481 gatacgatga aacgtgccga accgggcaaa aatgccattg ctcataccgt tgatcgcaac
     9541 ggcttatggc acgcgctgac gccgcaattt ttccctcgtg agctgttaca tgactgtctg
     9601 acgcgcgctc taaatgaagg cgcgactatt accgacgaag cctcggcgct ggaatattgc
     9661 ggattccatc ctcagttggt cgaaggccgt gcggataaca ttaaagtcac gcgcccggaa
     9721 gatttggcac tggccgagtt ttacctcacc cgaaccatcc atcaggagaa tacataatgc
     9781 gaattggaca cggttttgac gtacatgcct ttggcggtga aggcccaatt atcattggtg
     9841 gcgtacgcat tccttacgaa aaaggattgc tggcgcattc tgatggcgac gtggcgctcc
     9901 atgcgttgac cgatgcattg cttggcgcgg cggcgctggg ggatatcggc aagctgttcc
     9961 cggataccga tccggcattt aaaggtgccg acagccgcga gctgctacgc gaagcctggc
    10021 gtcgtattca ggcgaagggt tataccctgg gcaacgtcga tgtcactatc atcgctcagg
    10081 caccgaagat gttgccgcac attccacaaa tgcgcgtgtt tattgccgaa gatctcggct
    10141 gccatatgga tgatgttaac gtgaaagcca ctactacgga aaaacttgga tttaccggac
    10201 gtggggaagg gattgcctgt gaagcggtgg cgctactcat taaggcaaca aaatgattga
    10261 gtttgataat ctcacttacc tccacggtaa accgcaaggc accgggctgc tgaaagccaa
    10321 tccggaagac tttgtggtgg tggaagattt gggctttgag cctgatggtg aaggtgagca
    10381 tattctggtt agaatcctca aaaacggctg caatacccgt tttgtggcgg atgcactggc
    10441 gaaattcctg aaaattcatg cccgtgaagt cagcttcgct gggcaaaaag acaaacatgc
    10501 tgttacggaa cagtggttat gcgctcgcgt gccaggcaag gaaatgcccg atctgagcgc
    10561 ctttcaactg gaaggctgcc aggtgctgga gtatgcgcgg cacaagcgca agctgcgttt
    10621 aggcgcgctg aaaggtaacg cctttaccct ggttctgcgc gaagtgagca atcgcgatga
    10681 cgttgaacaa cgtctgatcg atatttgcgt aaaaggtgta ccgaactact tcggtgccca
    10741 acgttttggg attggcggta gcaacttgca gggcgcgctg cgctgggcgc aaaccaatac
    10801 tccggtgcgc gatcgcaata aacggagttt ttggttgtcg gcagcccgca gtgcgttgtt
    10861 taatcagatt gttgctgagc gcctcaaaaa agcagacgtt aatcaagttg ttgacggcga
    10921 tgcgctacaa ttagccggac gtggtagctg gtttgtagca accaccgaag aactggcgga
    10981 attacagcat cgcgtcaacg ataaagagct gatgataacc gccgcattgc cgggcagtgg
    11041 cgaatgggga actcagcgta aagcgctggc attcgaacaa gcagctgtcg ccgcagaaac
    11101 tgaattacaa gctttactgg tgcgcgaaaa agttgaagcc gcgcgcagag cgatgctgct
    11161 gtatccgcaa caattaagct ggaattggtg ggatgacgtc accgtagaga tccgtttctg
    11221 gcttccggcg ggtagttttg caaccagcgt tgtcagggaa cttatcaaca caacaggtga
    11281 ttatgcgcat attgctgagt aatgatgacg gggtacatgc acccggtata caaacgctgg
    11341 cgaaagcctt gcgtgagttt gctgacgttc aggtggtcgc ccccgatcgt aaccgcagcg
    11401 gcgcttcaaa ttctctgaca ctggaatcct ccctgcgcac gtttaccttt gaaaatggtg
    11461 atattgctgt gcaaatggga accccgaccg attgcgtcta tcttggcgtg aatgctctga
    11521 tgcgtccgcg cccggacatt gttgtgtccg gcattaacgc cgggccgaat ctgggggatg
    11581 acgttattta ttccggtacg gtagccgccg cgatggaagg ccgtcattta ggttttccgg
    11641 cgctggccgt ctcgcttgac gggcataaac attacgacac tgccgcggcg gtaacctgtt
    11701 caattttgcg cgcactgtgt aaagagccgc tgcgcaccgg acgtattctt aatattaacg
    11761 ttccggattt acccttggat caaatcaaag gtattcgcgt gacgcgctgc ggtacacgac
    11821 atccggcaga tcaggtgatc ccgcagcaag atccgcgcgg caatacgctg tactggattg
    11881 gcccgccggg cggtaaatgt gatgccggtc cggggaccga ttttgctgcg gtagatgagg
    11941 gctatgtctc catcacgccg ctgcatgtgg atttaactgc gcatagcgcg caagatgtgg
    12001 tttcagactg gttaaacagc gtgggagttg gcacgcaatg gtaagcagac gcgtacaagc
    12061 acttctggat caattacgtg cgcaaggtat tcaggatgag caggtgctga atgcacttgc
    12121 tgccgtgccg cgtgaaaaat tcgttgatga agcgtttgaa caaaaagcct gggacaatat
    12181 cgccttgccg ataggtcagg ggcagacaat ttcgcagccg tatatggtgg cgcgaatgac
    12241 cgaattactc gagctgacgc cgcagtcgcg ggtgctggaa attggcaccg gttcgggata
    12301 tcaaacggca atcctggcgc atcttgtcca gcatgtttgc tcggtcgaac ggattaaagg
    12361 tttgcagtgg caggcgcgtc gccgcctgaa aaatcttgat ttacataatg tttcaacccg
    12421 tcatggcgat ggatggcaag gttggcaggc acgcgcgccg tttgacgcta tcattgttac
    12481 ggcggcaccg ccggaaattc caactgcgct aatgacgcag ctggacgaag gcgggattct
    12541 cgtcttaccc gtaggggagg agcaccagta tttgaaacgg gtgcgtcgtc ggggaggcga
    12601 atttattatc gataccgtgg aggccgtgcg ctttgtccct ttagtgaagg gggagctggc
    12661 ttaaaacgtg aggaaatacc tggatttttc ctggttattt tgccgcaggt cagcgtatcg
    12721 tgaacatctt ttccagtgtt cagtagggtg ccttgcacgg taattatgtc actggttatt
    12781 aaccaatttt tcctggggga taaatgagcg cgggaagccc aaaattcacc gttcgccgca
    12841 ttgcggcttt gtcactggtt tcgctatggc tggcaggctg ttctgacact tcaaatccac
    12901 cggcaccggt cagctccgtt aatggcaatg cgcctgcaaa taccaattct ggtatgttga
    12961 ttacgccgcc gccgaaaatg gggacgacgt ctacagccca gcaaccgcaa atccagccag
    13021 tgcagccagt agctcagcag ccgctacaga tggaaaacgg acgcatcgtc tataaccgtc
    13081 agtatgggaa cattccgaaa ggcagttata gcggcagtac ctataccgtg aaaaaaggcg
    13141 acacactttt ctatatcgcc tggattactg gcaacgattt ccgtgacctt gctcagcgca
    13201 acaatattca ggcaccatac gcgctgaacg ttggtcagac cttgcaggtg ggtaatgctt
    13261 ccggtacgcc aatcactggc ggaaatgcca ttacccaggc cgacgcagca gagcaaggag
    13321 ttgtgatcaa gcctgcacaa aattccaccg ttgctgttgc gtcgcaaccg acaattacgt
    13381 attctgaatc ttcgggtgaa cagagtgcta acaaaatgtt gccgaacaac aagccagctg
    13441 cgaccacggt cacagcgcct gtaacggtac caacagcaag cacaaccgag ccaactgtca
    13501 gcagtacatc aaccagtacg cctatctcca cctggcgctg gccgactgag ggcaaagtga
    13561 tcgaaacctt tggcgcttct gaggggggca acaaggggat tgatatcgca ggcagcaaag
    13621 gacaggcaat tatcgcgacc gcagatggcc gcgttgttta tgccggtaac gcgctgcgcg
    13681 gctacggtaa tctgattatc atcaaacata atgatgatta cctgagtgcc tacgcccata
    13741 acgacacaat gctggtccgg gaacaacaag aagtgaaggc ggggcaaaaa atagcaacca
    13801 tgggtagcac cggaaccagt tcaacacgct tgcattttga aattcgttac aaggggaaat
    13861 ccgtaaaccc gctgcgttat ttgccgcagc gataaatcgg cggaaccagg cttttgcttg
    13921 aatgttccgt caagggatca cgggtaggag ccaccttatg agtcagaata cgctgaaagt
    13981 tcatgattta aatgaagatg cggaatttga tgagaacgga gttgaggttt ttgacgaaaa
    14041 ggccttagta gaagaggaac ccagtgataa cgatttggcc gaagaggaac tgttatcgca
    14101 gggagccaca cagcgagtgt tggacgcgac tcagctttac cttggtgaga ttggttattc
    14161 accactgtta acggccgaag aagaagttta ttttgcgcgt cgcgcactgc gtggagatgt
    14221 cgcctctcgc cgccggatga tcgagagtaa cttgcgtctg gtggtaaaaa ttgcccgccg
    14281 ttatggcaat cgtggtctgg cgttgctgga ccttatcgaa gagggcaacc tggggctgat
    14341 ccgcgcggta gagaagtttg acccggaacg tggtttccgc ttctcaacat acgcaacctg
    14401 gtggattcgc cagacgattg aacgggcgat tatgaaccaa acccgtacta ttcgtttgcc
    14461 gattcacatc gtaaaggagc tgaacgttta cctgcgaacc gcacgtgagt tgtcccataa
    14521 gctggaccat gaaccaagtg cggaagagat cgcagagcaa ctggataagc cagttgatga
    14581 cgtcagccgt atgcttcgtc ttaacgagcg cattacctcg gtagacaccc cgctgggtgg
    14641 tgattccgaa aaagcgttgc tggacatcct ggccgatgaa aaagagaacg gtccggaaga
    14701 taccacgcaa gatgacgata tgaagcagag catcgtcaaa tggctgttcg agctgaacgc
    14761 caaacagcgt gaagtgctgg cacgtcgatt cggtttgctg gggtacgaag cggcaacact
    14821 ggaagatgta ggtcgtgaaa ttggcctcac ccgtgaacgt gttcgccaga ttcaggttga
    14881 aggcctgcgc cgtttgcgtg aaatcctgca aacgcagggg ctgaatatcg aagcgctgtt
    14941 ccgcgagtaa gtaagcatct gtcagaaagg ccagtctcaa gcgaggctgg ccttttctgt
    15001 gcacaataaa aggtccgatg cccatcggac ctttttatta aggtcaaatt accgcccata
    15061 cgcaccaggt aattaagaat ccggtaaaac cgagaatggt cgttaacact gtccaggttt
    15121 tcagaccgtc tgctaccgac aaccccagat atttggtcac aatccagaac cctgagtcat
    15181 taatatgtga cgcgccaagc ccaccaaagc aggctgccag cgtcaccaat acgcactgaa
    15241 tcggattcaa tcccatcacc gcttctgaga gtaacccgcc ggttgtcagg attgctacgg
    15301 ttgctgaccc ctgcgatgca cgcagagcca gtgaaataat aaatgcggct ggtaacagag
    15361 gcaggtcaat catttgtagc atattggcaa gggctttgcc gacgcccgat tccaccagca
    15421 ctttgccaaa tacccctcca gcaccagtaa ccaaaatcac taccgccgca gtgggaaggg
    15481 ctgagcccat aatgtcgctg gtgtgttgta agctccagcc gcgacgtaaa gccaataacc
    15541 agaatgccag caccagcgca atcattagag ctaccattgg tgagccgatc agctgtagcg
    15601 taccaagcag gggatgcgaa ggcggcatca gtgttgcgga aaccgtaccc gtcatgataa
    15661 tcgcgatagg aataacaatt agcgaggtga ccagcgcgac gcccggtgga tttattttat
    15721 cgcttaattt tgtcgcgcct tcctcactgg ccggagccag ttgcatctgt tccagtactt
    15781 ctactgacat cgcatattgg cgcttattga ttattttcgc tgcaaagtag ccaacaatcc
    15841 ctacgggaat agaaatcgca ataccgatga tggttagcca gccgatgtct gcgtggagta
    15901 accccgctgt ggcgacaggg cctggatgcg gcggtactgc cacatgaaca gttagcatga
    15961 ttccagcgac aggcaggcca aatttgagtg gcgatatttt ggcaaccttg gcaaaaccgt
    16021 aaatgattgg cgcaagaata ataaagctga catcaaagaa gacgggaata ccgaggaaga
    16081 acgctgccag agtcagcgca gcgatagttc gtttgtcacc taacttgcga ctgaaataat
    16141 tagccagtga ctctgcacca ccagagtgtt cgatcatacg ccccagcata gcgcccagac
    16201 caataataat agtgacggaa ccaagcacac cgcccatccc ggcgatcatc actttaccca
    16261 cttcgcccgc cggtataccc gccgcaagtg cgactaacag gctgacgagg agcagagcaa
    16321 cgaatggttg tacctttgcc ttgatgacca gcagcaacag catgattacg ccagctaacg
    16381 caatgcataa caatgtaatt gtggacatgg gaaaccctgt ctgaaagtta tagttaacct
    16441 accccatccg tagatggggg gatgtatggg tacgttgtaa ttagggattt aacgaattag
    16501 cgccaggcgt caaaccagcc aagcccttct tcggtgaggc cacgaggttt atattcacaa
    16561 ccgatccagc cctgatatcc cacctcatcg aacaggcgga acagccacgg atagttgatt
    16621 tctccatcgt ccggttcatg tcgatcaggt agtccggcaa tttgtacgtg cgcatatttc
    16681 ccagcgtagt cgcggattaa atgcgtcagg ttgccatcta ctttttgcgc atgaaaagta
    16741 tctagttgaa taaacacgtt atctcgcgca acctcttcaa caatagccag tgcctgatac
    16801 tggctggaga agagataatg aggcttaacg tcggggctga gtgcttcaac taatattcgc
    16861 ttgccgtgtg gcgcaaagcg gtcggcagcg tagcggagat tatcgataaa tactgcccgg
    16921 taccgttcag catcttcgcc agcgggcacg acgcctgcca tcacatggac ttgttcacaa
    16981 ttgagcgcca atgcatattc cagtgccagg tcgatgtctg cgtgtgcttc gtgctcacgt
    17041 ccgggaaggg cggataatcc ccattccccc gcattaatat ctccgggagc ggtattgaac
    17101 agcgccagtg tcagatggtt ttgctccagt tgcttttgga tttgcagggt ggagtagtca
    17161 tagggaaaca gaaattccac agcatcgaac ccggcttttc gcgctgcggc gaagcgttca
    17221 ataaaaggca cttcggtgaa catcatggat aaattagctg caaaacgagg cattgcatta
    17281 actccttaat tccgcaattt cacctgcggt cagataacgg atcgggcggt caccgagaat
    17341 aaaaatcagc tttgccgttt cctccagctc ttccatattg ttggcggctt cttgcaggct
    17401 ttcaccgcaa accactgggc catgatttgc cagtaaaaaa gcctgattgt ctgctgccag
    17461 ttccgccaga tcctgtgcga tgcgtttatc gcccggtcgg taatagggca ccagcgggac
    17521 atttcccatc cgcatcacca cgtatggtgt gaacggacga ataacgttgc tgctgtccag
    17581 cccttgcagg caggaaagcg ccgtcgacca tgtgctgtgc aaatgcacca ccgctttaca
    17641 gcgcggattg ttgcgataca gcgccagatg aaagagcacc tctttcgagg gtttgtcacc
    17701 acttaaccat tcgccatccg cggcgacttt ggaaagccgc tgcggatcga gattgcccag
    17761 gcatgaacct gtcggtgtcg ccagtaaatt cccgtcaggt aaaagcagcg acagattgcc
    17821 agccgaaccg gttgcatagc cgcgctgaaa gaatgaactg gcaatccgcg tcatctcctc
    17881 tcgcaaagac tgctctactt ttgcgaaatc gctcatgata aaaactctct ttgggctcgt
    17941 gaaaaaaagg cttcatcacc gaagttgcca gatttaaggg cgagtgagac aggcttatcc
    18001 agtgcgttta cccacggcac gccgggggaa atggttgggc caatatgaaa cccttttatg
    18061 cccaggctct gtgtgactac gccggaggtc tcaccgcctg cgacaataaa gcgtgtcacg
    18121 ccttccgctg ctaaccgcgc cgctagttga gaaaacagag tttctactgc ctgactggct
    18181 ttttgtgcac cgtattgctg ttgaattgct gccaatgcgt cagtgctggc ggtggcaaaa
    18241 accagtggag caagtacact ttcctggccc agaacccact ctgccagttc gtgtgcataa
    18301 gcggccagag tttcggttga gaggcagcgt gccacatcaa cttcacgggc tggtgcaatt
    18361 tgacggtaat gtgccacctg gcggttggtc atttgagagc atgaaccgga gagcactacg
    18421 ccgcgcccag cgagcggatg ccctgcttcg cgagcctggt taccgttttc ttgcgccact
    18481 gccgggccag gccaatcgcc agaccagaac cgcccgttac cagtggggca tcgcgcaagg
    18541 cttctccctg aatttccaga tggtgttcgg tcagcgcatc aagcaccgcg tagcggtagc
    18601 cctcttgctg taagcgagcc agctcttgac gaacggcatc cacaccttgt tcgaaaacat
    18661 gtgccgaaac gacgccgcag cgccctgtgg attgcgcttc aaccagacgg ggaagatagc
    18721 tgtcggtcat gggatttacc gggtgatggc gcatcccgga ttcggccagc agttgattca
    18781 ttacgaacaa atacccctga taaaccgtac gtccgttgac cgacagggcc ggagagaaga
    18841 ccgtaaacgg cgtgtcgaga gcatccatta atgcatcggt aaccgggccg atattacctt
    18901 tcgccgtact gtcgaaagta gagcagtatt tgaaatagat ctgtttgcaa ccttgctgtt
    18961 gcaaccagct cagagccgcc agcgattgct gtgtggcttc aaccaccgga caggagcgcg
    19021 ttttcaggct gatcaccagt gcgtcgattg cttccggcat tttacctgtt ggaacaccgt
    19081 taatttgtac cgttggtaga ccgttttcca ccagaaaact ggcgatatcc gtcgcgccgg
    19141 taaaatcatc ggcgataacg ccaatcttga tcatgatttc gctcccggta gagtgatgcc
    19201 agagaaaatc ttgataactg cgctatcgtc ttctttcccg taacctgcgt tactggcgct
    19261 ggtgaacata ttcaatgctg ttgaggccaa tggcagcggg aagtgcaggg ctttggctgt
    19321 atcggcaacc agaccaagat ccttaacaaa aatatcgacg gctgaatgcg gggtgtaatc
    19381 gccatccacc acatgacgca tccggttttc gaacatccag gaatttccgg cggcattggt
    19441 cacgacgtca tacatcacat ccagcgggat ccccgcacgg gctgcaagtg ccatcgcttc
    19501 ggctccggca gcaatatgta cgcccgctaa caactggtga ataattttta cggtcgaacc
    19561 tagtcccggt tctgcaccta tgcgataaac ttttccggca acggcttcca gcacgggtgc
    19621 cagtcgttca aaggcaatat cgctaccgga ggccatgaca gtcatttcac cgttagcggc
    19681 ttttactgca tcaccagaaa ctggcgcatc cagcatttcc agatcgaatc cagccagagc
    19741 ggtagcaatt tcttgcgcat cagcactagc gatagtggaa gaaaccatta ctgccgtacc
    19801 gggtttcaga tgttgtgcaa cgcctgtttc accaaacagc acctgtttaa cctgggccgc
    19861 attgaccacc agcaccagca gagcgtccag tttttcggca aacgtcgcgg cgttatcaga
    19921 aaccccgcaa gcacctgcct ctttcaacgt agcgcaggca ttgctgttca ggtctgcgcc
    19981 ccaggtagaa agacctgcgc ggacacatga cagtgctgct cccattccca ttgcccctaa
    20041 gccaacgata ccgacatgaa actcagatcc cgttttcatc tgctctcctt gttaatttaa
    20101 gtgatatttt gtttgatatt gtgaatataa gcgctggaag ataacgatat ggtgagcaga
    20161 ttcacataaa ttaacattgc gtgttatttt atgtgaacta agcgttagtt gcgccgcgca
    20221 cgtttcgcag gcaaatagcg tagaatgtca gcaggacaaa ggaaggagca aaagttgata
    20281 cccgtagagc gtcgccaaat catccttgag atggtagctg aaaaaggcat tgtcagtatt
    20341 gctgaactaa cggacagaat gaatgtatca catatgacca ttcgtcggga tttacaaaaa
    20401 ctggagcagc agggagccgt tgtgctggtg tccggaggcg tccagtctcc gggacgcgtg
    20461 gcgcatgaac cttctcatca ggtaaaaact gcgctagcaa tgacgcaaaa agcggctatt
    20521 ggcaagctgg cggcaagtct tgttcagccg ggaagttgta tctatctgga tgcgggaacg
    20581 accacgttag cgatagcaca gcatctgatt cacatggagt cactgactgt ggtcacaaac
    20641 gatttcgtta ttgcgaacta cttgctcgac aacagtaatt gcacaattat tcacactggc
    20701 ggtgcagtgt gtcgggaaaa ccgttcctgt gtcggggaag ccgctgcgac catgctgcgc
    20761 agcctgatga ttgatcaggc ttttatttct gcatcgtcat ggagtgtgcg ggggatttct
    20821 acgccagcgg aagataaagt cacggtgaaa cgggcgattg ctagtgccag ccgccagcga
    20881 gttttggtct gtgatgcgac gaaatatggt caggtggcga catggctggc gttaccatta
    20941 agcgagtttg atcagattat tacagacgac ggtttgccgg agagtgccag tcgcgcgctg
    21001 gcgaagcagg atctctcttt gctggtagcg aaaaatgaat aatggcctgc aataacatct
    21061 ggttactcat gcttcacaga agaagcatga gactacctta ttttataaaa tgacagccgc
    21121 ccacttttcg gcgatccggt atcaatataa atctggttag cgaacgtctg aatgttatca
    21181 aacatcatat gtccaaatat aaaataatca gcgccgttta tttgttgtaa ctcgccatta
    21241 agcgatttct gcacacgata aacaggccag agtaattcgc tctccgctat ttctttgcca
    21301 aagagatatt catcacccgg ataatctgca tgtgcgatga catattttat tatgtcgtta
    21361 gtgatttcaa taatatgtgg aaggtgatgg aatttcagca acagatctat tgcctcttgt
    21421 tgctctgaat catttaaatc gaaaaaccag tcaccaccgc tggcaagcca catattgcca
    21481 tcgccggtcg cgaacgcatc cagctccatt gcttcgtggt tgcctttaac ggagataaac
    21541 cagggctggt ttagcaggcg caggacgtca agactctccg gcccacgatc aatattatcg
    21601 ccgacagaaa taagtaagtc ggtttcaggg caaaaagaga gttgatgtaa gcgggattgt
    21661 aataattgat attcaccatg tatatcacca acgacccata tatggcgata gtgatgggca
    21721 ttaatttttt gatagcgtgt agatggcatg gttttaccct gtaaaataag ctttcctatt
    21781 atacaggggg tttttatttt attcgtcagt tgtcgttaat attcccgata gcaaaagact
    21841 atcgggaatt gttattacac caggctcttc aagcgataaa tccattccag cgcctgacgc
    21901 ggagtcagtg aatccgggtc caggttttcc agagcttcga ccgctggcga ggtttcttcc
    21961 ggtactgaca gcaaagacat ttgcgtacca tccacttgcg tagcggcggc gttcggcgaa
    22021 atgctttcca gctcacgcag tttttgccgt gcgcgcttaa tcacctcttt tggcacgcct
    22081 gccagagctg caaccgccag gccgtagctt ttgctcgccg cgccatcctg cacgctatgc
    22141 ataaaggcaa tggtgtctcc gtgctccagc gcatcgagat gcacgttggc gacgtcttcc
    22201 attttctccg gtagctgggt cagctcgaaa tagtgggtgg caaacagcgt caatgcctta
    22261 atcttattcg ccagattttc tgcgcacgcc cacgccagcg acagaccatc gtaggtggac
    22321 gttccacgcc cgatctcatc cattaacacc agactgtatt cggtggcgtt atgtaaaata
    22381 ttggcggttt cagtcatctc caccataaag gttgagcgcc cggaagccag gtcatctgcc
    22441 gcgcctacgc gggtaaagat gcgatcgata ggtccaatct cgactttttg tgccggtaca
    22501 tagctgccaa tgtaggccat cagcgcaatc agtgcggtct ggcgcatata ggtactttta
    22561 ccgcccatgt tcgggccggt aataatcaac atacggcgct gcggcgacag attcagcggg
    22621 ttagcgataa atggctcatt cagtacttgt tcaactaccg gatggcgacc ttcggtaatg
    22681 cgaatgcccg gtttatcaat gaaggtcggg caggtgtagt tcagggtata ggcccgttcc
    22741 gccaggttaa ccagcacgtc gagttccgcc agcgcgctcg cgctctgttg caacgcttcc
    22801 agatgcggca acagcaggtc gaacagctct tcataaagct gtttttccag tgccagtgct
    22861 ttgccttttg aggtgagaac tttatcttcg tactctttta gctctggaat gatgtagcgc
    22921 tcggcgtttt tcagcgtctg gcgacgcatg tagttgatgg gtgccagatg gctttgccca
    22981 cggctgattt gaatgtagta gccgtgcacc gcattaaagc caactttcag cgtgtccagg
    23041 ccggtacgtt cacgctcgcg gacttccaga cgctccagat aatcggtcgc gccgtcagcc
    23101 agcgcgcgcc actcatccag ctcttcgtta tagcccgatg cgataacacc accgtcgcgt
    23161 accagcaccg gcggtgtgtc gatgattgct cgctccagca gatcgcgcag ctcggcaaac
    23221 tcgcccatct tctcacgtag cgcctgtacc ggtgcactat cgacagtttc taactgtgca
    23281 cgcagctccg gcagttgctg gaaagcgtgg cgcatacggg ccagatcgcg tgggcgagca
    23341 gttcgtaaag ccagacgtgc cagaatacgt tcaaggtcgc cgacctgacg cagtaccggc
    23401 tgtagcccgg cggtgaaatc ctgcaatgcg ccaatagttt gctggcgctc aagcaacacg
    23461 cgggtatcgc gcactggcat atgcagccag cgtttcagca tacgactacc catcggcgtg
    23521 acggtgcagt cgagcacaga agccagcgta ttttccgcac cgcccgccag gttctgagta
    23581 atttccaggt tacgacgtgt cgcggcatcc ataatgatgc tgtcctgctc acgttccatg
    23641 gtgatagaac gaatatgcgg cagggtcgtg cgttgggtat ctttcgcata ctgcaacaga
    23701 caaccggcag cacaaagtcc gcgcggcgcg ttctcgacgc caaaaccgac cagatcgcgg
    23761 gtgccaaatt gcagattcaa ctgctggcgc gcggtgtcga tttcaaactc ccacagcggg
    23821 cgacggcgca ggccgcgacg gccttcaatc agcgacatct cggcgaaatc ttctgcatac
    23881 agcagttccg ccggattagt gcgttgcagc tctgccgcca tcgtttcgcg gtcggccggt
    23941 tcgctcaggc gaaaacgccc ggagctgata tccagcgtcg cgtagccgaa acctttgctg
    24001 tcctgccaga tagccgccag caggttgtcc tgacgctcct gtaacagggc ttcatcgctg
    24061 atggtgcctg gcgtaacgat acgcacaact ttgcgctcaa ccggaccttt gctggtcgcc
    24121 ggatcgccaa tttgttcgca gatggcaacg gactctccct gattcaccag tttggcgaga
    24181 tagttttcca ccgcatggta gggaatcccc gccatcggga tcggctctcc cgccgaagca
    24241 ccgcgtttgg tcagtgaaat atccagcagt tgcgacgcgc gttttgcgtc gtcataaaac
    24301 agttcataaa aatcacccat ccggtaaaac agcaggatct cgggatgctg ggctttcagc
    24361 ctgagatact gctgcatcat gggcgtatgg gcgtcgaaat tttctattgc actcatgggg
    24421 ttatgtccgg ttccctgata ttaaatgggg tgtgatggtt tttattttcg ttgtaatcac
    24481 ataaggcgca catgataccg gagttaatca tttcagggga agaagcgaac ggcgagttct
    24541 tgatgctggt tccgggaaag atgctgtttt gcagcttttg cgtgatgctt atgaagcaag
    24601 tacgcaaaat tgcgtcggtt gaataaacct cctccttcag agccatagtc ccctttcaaa
    24661 ttaagttgta atataaatac accttaagag tatggaggca gcatgtccgg caagcgtatc
    24721 tctcgtgaaa aactgacgat taaaaaaatg atcgatcttt atcaagcgaa atgcccgcag
    24781 gcgtcagcgg agccggagca ttacgaggcg ttgtttgttt acgcgcaaaa gcggctggat
    24841 aaatgtgtgt tcggcgaaga gaaaccagcc tgtaagcagt gtccggtgca ctgttaccag
    24901 cctgcaaagc gtgaagagat gaaacagatt atgcgttggg ccgggccgag aatgctgtgg
    24961 cgtcatccga tcttaaccgt gcgtcatctc atcgatgaca agcgtccggt gccagaactg
    25021 ccggaaaaat atcgtccgaa aaaacctcgt gaatagtatg ccggacaggc agatctgtcc
    25081 ggcaatttgc agttaaatca atgccgattt atcaattccc agccgcttca tccgtgacag
    25141 cagggtcgtg cgtttcagcc ccagacgttg cgcagcgcct ttaggcccgg caaccacgcc
    25201 gttggtttct ttcagcacgc gaacaatcaa ctgatattca tcttcgccct cctgagcgac
    25261 aaccgttgcg gcaggcggcg tttcaggctc cggtaaagca atatctggca atgacagctg
    25321 cagcacgttg ccgcgtgtta gcaatactgc gcgctcaatg acgttttcca gttcgcgtac
    25381 gttacccggc cactccatgt tgctcaaggt gcgcagcgtc tcggctggaa tgctgtcgat
    25441 attgcgcccc agacgacggg caattttgaa ggtaaaggct ttcgccagca gcggaatatc
    25501 ttccggacgc tcgcgtagtg gcggcaggtg aatcgggaat acgttcaggc ggtaatagag
    25561 atcgctacgg aactctcggt cggcgaccat ttttttcaga tcgcggttag tcgcggcaat
    25621 taaacgtacg tccgtctgaa tgattttgtt gctgccgaga cgttcaaact cctgttcctg
    25681 caatacacgc agcaacttcg gctgtaactc cagtggcata tcgcccactt cgtcgaggaa
    25741 cagggagctt ttatccgcca gttcaaaacg accaatgcgc tgggcgctgg caccggtaaa
    25801 agccccacgc tcatgaccaa acagatcgct ttccagcaat ccggcaggca tcgccgcgca
    25861 gttcattttg accatgcggc gattattacg cccactgaga ttatggatcg cgcgggcaat
    25921 cagctcttta cccgtgccag tttcaccgag gatcagcacg gtactgtcac tttgcgccac
    25981 catttcaact tgtttaagca cgctgtacat ggcttcgctg cggccaataa tctcgccaaa
    26041 ttcactataa acattgttga gctgctcggt cagggcgagg ttttcatcaa ccagccgttc
    26101 tttcagacga tggatttcct gataggcgag ggcgttatcg acagcgattg ccacacgttc
    26161 ggcaatctgg cgcagcaaat tcagattggt agtggtaaac actttctctt cgcattgcgc
    26221 cagtttcagc acgcccagca tggtgtcgcc agacatcagc ggtaacaggc acaaggtttg
    26281 aatctggttg ccccaggtgt cgaacaacat gcgttcatag ggggctaaat cgtcccgctc
    26341 gtggagattg atcagcagca tctctttact tttgaacacg cgttcggtga gggttccggc
    26401 ttcatcgact tcgctctgtt cgtgggcggg atgttgttta tcaagatagt gagtggagta
    26461 gatgttgagt ttgtttttac ggtggctgcg taagacgata ctgatatcgt caatgtcgaa
    26521 atagtaatgg atttctttgg cgacttcgct gaccagttcg tccatatcca ggcgggaaag
    26581 caccgcgttg gtaatggcaa ccaggatgcg gaagttatcg cgctcccggc ataacaactc
    26641 atagtcgaca ttgttgacga cgcggctctg gatttgttcg gtgacgacag aaacgatctg
    26701 cgtaaatgtt tgcagacgat tgaactcttt ttcgctccag ggacgatcgt cataacgaat
    26761 aaattcacag ccaccaaaaa tatgcccttc cgccgccagt ggcatcaggc aatagtgacc
    26821 aaatttgggg tatagcccac cagcgaccag ctgcggccag gtttcacaaa attcttcgta
    26881 actgcaatgc agcgtatcag ggcgcgacaa aatgcggcgt accggaccgt gtgccagaac
    26941 agtttcgtct tcatatttaa tgggggtgtc tttttcacgc gatgcgtaat aagacgcacg
    27001 ttgagtctgc gcttgccaca acacaatagc cgcgttgtcg gcgagcgcag aacgctttac
    27061 cagttgcgaa agagcctcac acagcgaggc cagatcgggc tgctgcaata gtgtccgagt
    27121 gatgtcgaac aacccttgct gtccgagatc actcatcggt gtatatgaca ttttgttctt
    27181 ccaggaaagc accgcaaagc ggtgcaaaag gtcattgact aactatagga gatttagaat
    27241 tttattagca tatacgcgga agcggttcgg cgtgtggtaa atcgagggtt cgtttcacac
    27301 catacagacc ggcaagacga acacctttac gttccaccac ttcaccaatt agcgccgcgt
    27361 ctttccccag tggatgggaa tgtaacgctg ccagcacttg ctctgctgcg ttgcgttcaa
    27421 cagctattac tagtttgcct tcgttggcaa agttaagggc gtccagtccc agcagttcgc
    27481 aaacgccgcg cacagcaggt ttaaccggca gtgctgattc agaaatttca ataccacaac
    27541 cgcaggctgc cgcgaactca tgaaccaccg cgtttacacc accacgggtg gcatcacgca
    27601 gcgctttcac gccgggaatg tcacgcagcg tctgaataag cggcgtcagc accgcgcagt
    27661 cgctgaccag ttcaccatcc agccccagtt gttcacgcag gttaaggata gtcgccccgt
    27721 ggtcgccgag tgtaccgcta accagcaata catcgcctgc ggttagcgtc tgtgcgcccc
    27781 agtgaatatt cgccggaatt gcgcccatgc cagcggtgtt gataaacagt ttatctgccg
    27841 cgccgcgctg caccacttta gtatcgccag taacgatggc aatgcctgcc gtgcgggcgg
    27901 tttctgccat gctggtcact acggctttca gtgtctccat cggcaatcct tcttcgagga
    27961 taaagccaca ggagagatag cacggaatag cgccactgac cgcaacgtca ttcgcggtgc
    28021 cgcaaatcgc cagcttgccg atattaccgc caggaaagaa cagcgggtca atgacgtaac
    28081 tgtcggtgga gaacgccaga cgatcgcctt ctgctaccag ctgcgccaga tcaagacgtg
    28141 cctggtcttc ctgctctgcc agccacgggt tggcaaaggc ttccataaac aggctgttga
    28201 ttaattgctg catcgcctgg ccaccgctgc cgtgggcgag ttggatatta ttcacgcttc
    28261 actctcctgc tgacgatact gataccacgc ggcgcacgct ccttcggagg agaccatcag
    28321 tgcaccaaat gcggtttgag gattacaggt gttaccaaac agcgggcatt gatgcggctt
    28381 acatttgccc gttaatacct caccacaacg cgcgcgcggg tcatcgcaga cctgctgcgg
    28441 tgccgggcgg aaatgtgctt cggcatcgaa tcgttgataa tccggcgtca ggtgcacgcc
    28501 agaagattca atcacgccta agccgcgcca ttcgctgtcg ccgttgacac agaacacatc
    28561 ggcaatcgcc tgttgcgcca gcaggttacc ggcatccggt accacccgac gatactgatt
    28621 ctctaccttg ctgtgggccg ctattttctg ctccaccagc atcaccacgc cttgcagtag
    28681 atcaaggggt tcgaaaccag cgaccaccag cggacgatga aaatcgctgg cgataaaatt
    28741 ataggcatca gtgccgataa ccatactaac gtggcccggc gcgaggaacg cgtcgatacc
    28801 gttatccggc tgttccagca aactgcgcag cgtcgggata agcgtaatgt gctggcagaa
    28861 gaagtaaaaa ttctgcacat cacgcgcttt cgcctgttgc agagtgatgg cggtggtcgg
    28921 catggtggtt tcaaaaccta agccgaagaa caccactttg cgggttggat tctcctgcgc
    28981 cagtttcaac gcatccatcg gcgagtaaac gatgcgcaca tcggcaccgc gtgcttttgc
    29041 ctgcaacagc gatccctgtt tccctggtac gcgcatggca tcgccaaagg tacagaagat
    29101 gacttccgga tggctggcaa tctccacgca ggtgtcgatt ctacccatcg gcagtacgca
    29161 caccgggcaa cccggaccgt ggataaactc aacgttttca ggcagtaact ggtcgaggcc
    29221 gaatttaaaa atagcgtggg tatgaccacc gcacacttcc ataatccgca gagggcgttc
    29281 ggcggtgtaa gagagatgtg aagcacgttc gcgcagatgc tcaattaact gcatcacctg
    29341 ttccggcgcg cgatattcat caacaaaacg cattattttt cctcgccata caacagcgcg
    29401 ccgacatccg gctcaacgtc aaacatgttt tgtaaggcgt cgagagtgtc gcgtgcttcg
    29461 gcttcagtaa ttacgctcat ggcaaagcca acgtgtacca gtacccactg gcccacgcgc
    29521 ggctgaccgt tttcatcgca gctgccgact aacgttaaat cgacatcgcg ctgaatgccg
    29581 cagacgtcga cttttgcctg gttgccgtca atggtgcgga tctggccggg aacgcctatg
    29641 cacatcgctg tgtctccagc cagttcagcc actggtccat cccttcaccg ctggtggcgg
    29701 aaataaggat gatttcaatt tctggattga cttcgcgggc gcaggcgatg cacttctcaa
    29761 cgtcaaagtt gagatacggc aacaggtcaa ctttgttgag cagcatcagc gaggcggcgg
    29821 caaacatatg cggatatttc agcggtttgt cttcaccttc ggtaacggaa agcaccgcca
    29881 ctttgtgttt ttcaccgaga tcgaagctgg ccgggcatac gaggttgcca acgttttcga
    29941 taaacagaat accgttatcg tccagtggca gacgcggtgc ggcgtcggca atcatctgtg
    30001 cgtcaagatg gcagccttta cctgtgttca cctgaatcgc tggtgtgccg gtagcgcgaa
    30061 tgcgtgcggc atcgttcacg gtttgctggt caccttcaat aaccgcgcac ggaacgctgt
    30121 ctttcaggcg cattagggtt tccgtcagca gggtggtttt accggaacct gggctggaaa
    30181 ccaggttgag caccagttgc ttgcgggcag caaagcgcgc gcggttgcgt tcagccagac
    30241 ggttgttttt gtccagcacg tcaatttcga cttccagcat ccgacgctgg ctcatgcccg
    30301 gtgcgtgagt gcccgcttcg ccatgaccgt aatgcaggtc gccttcttca gtctggctgg
    30361 gggtaaattc aggcgcttta atgccggtga ttttcatctt cgggcgtgcc gccggggcaa
    30421 atggcgcgct acgaaacgcg gaatgagggt tatgttcatc accctcgata tacaggttgc
    30481 cttcaccgca accgcatgtt gtacacatcg ctcactcctg gtctatttct atccgccgaa
    30541 tctgtaaacc gtcgtctgcc acaatctgta gcatgtcacc atgacactgt ggacagcggc
    30601 ggacgcgctg ggtcaatagc gtcacatact gttggcatgt ttcacaccag cattcgacct
    30661 cttgttcttc gaggtgcagt ttacaacctt ccgccacgct gccgcggcaa accagatcaa
    30721 aacaaaaggc aagagagctg gtttcgacac aagaaaatgc gccaattttg agccagaccc
    30781 cagttacgcg ttttgcgccg tgttttgcgg cctgctgttc gatcaattcc agtgcccgtt
    30841 ggcagagggt tatttcgtgc atatcgcctc ccattaacta ttgccagcta caagcaataa
    30901 ttgtgccagt gttgattatc cctgcggtga ataatgtcga tgatgtcgaa atgacacgtc
    30961 gacacggcga cgaaattcat ctttagctta aaaatcgttt taataacaat aaattaaaaa
    31021 ttggcacaga aaatgcttaa agctggcatc tctgttaaac gggtaacctg acaatgacta
    31081 tttgggaaat aagcgagaaa gccgattaca tcgcacagcg gcatcgtcgc ctacaggacc
    31141 agtggcacat ctactgcaat tcgctggttc aggggatcac gttatcgaaa gcgcgcctgc
    31201 atcacgccat gagctgcgcg ccggacaaag aactctgttt cgtccttttt gaacattttc
    31261 gtatttacgt caccctggcg gatggcttta acagccacac catcgagtat tacgtcgaaa
    31321 caaaagaggg cgaagacaaa cagcggattg cgcaggcgca actgagcatt gacggcatga
    31381 ttgatggcaa ggtcaacatc cgcgatcgcg aacaggttct ggaacactat ctcgaaaaaa
    31441 tcgctggcgt ttacgacagc ttatacaccg ccattgaaaa caatgtgccg gtgaatttaa
    31501 gccaactggt aaagggacaa agcccggcag catgagctga ggctttgccc gttttgcagg
    31561 cgttacgcct gtttggggat gggcgtgtcg atgagtgtcg aaaatgacat ttcatcggca
    31621 tgttttcgtc aaaaatgaca atcacctgag gaatgcctgg tgaatcgttt tgtaattgct
    31681 gactccacgc tctgtatcgg ctgccacact tgtgaggcag cctgttcaga gacgcatcgc
    31741 cagcacggcc tgcaatcaat gccgcgcctg agagtgatgc tgaatgaaaa agaatctgcg
    31801 ccgcagctct gtcaccactg tgaagatgca ccctgcgcgg tggtctgccc ggttaacgtc
    31861 atcacccgcg ttgatggggc cgtgcagttg aatgaaagcc tgtgcgtaag ctgcaagctg
    31921 tgcggcatcg cctgcccgtt tggcgcaatt gaattttccg gcagccgtcc gctggatatt
    31981 cctgcaaatg ccaatacccc gaaagcgcca ccggcaccgc ctgctccggc gcgtatcagt
    32041 acgctgcttg actgggtgcc gggtattcgc gcgatcgccg tcaaatgtga cctttgtagc
    32101 tttgatgaac aaggtccggc ctgcgtgcgg atgtgcccga ccaaagccct gcatctggtg
    32161 gataacaccg atatcgcccg cgtcagcaaa cgtaagcgtg agctgacttt taatacggac
    32221 tttggcgatc tcaccttgtt tcagcaggct caaagtggag aggctaaatg agcgcaattt
    32281 ccctgatcaa tagcggcgtg gtgtggtttg tcgccgccgc tgttctggca tttctctttt
    32341 cttttcaaaa gacgctaagt ggctggatag ccggaattgg cggcgcggtt ggtagcctgt
    32401 atacggcagc cgcgggcttc actgtgctga ctggcgcggt tggcgtgagc ggtgcgctgt
    32461 cgctggtaag ctacgatgtg caaatctccc cgcttaacgc gatttggctg attacgctcg
    32521 gtctgtgcgg tctgtttgtc agcctctaca acattgactg gcatcgccac gcgcaggtga
    32581 agtgcaacgg cttgcagatc aatatgttga tggctgctgc cgtctgcgcc gtcatcgcca
    32641 gcaacctcgg catgttcgtg gtaatggccg aagtcatggc cttgtgtgcg gtgttcctca
    32701 ccagcaacag caaagagggc aaactgtggt ttgcgctggg gcgtcttggc actctgctgc
    32761 tggcgattgc ttgctggctg ctgtggcagc gttatggcac gctggatctg cgcctgctgg
    32821 atatgcgtat gcaacagctg ccgctcggtt ccgatatctg gctgctcggt gtgattggct
    32881 ttggcctgct cgccgggatt attccgctgc acggctgggt gccgcaggca catgctaacg
    32941 cctctgcgcc agctgccgcg ttgttttcta cggtagtcat gaaaattggc ctgctgggca
    33001 ttttaaccct gtcactgctg ggcggtaatg caccgctgtg gtgggggatc gtgctgctgg
    33061 tgctcggcat gatcaccgcg tttgtcggtg gtctgtatgc gctgatggag cacaacatcc
    33121 agcgcctgct ggcttaccac accctggaaa atatcggcat tatcctgctg gggctgggcg
    33181 ctggcgtaac gggtatcgcg ctcgaacaac cggcgctgat tgctcttggc ctggtcggtg
    33241 gtctgtacca tctgcttaac catagcctgt tcaaaagcgt actgttcctc ggggcgggga
    33301 gcgtctggtt ccgtaccggt catcgcgata tcgaaaaact cggtggtatt ggcaagaaaa
    33361 tgccggttat ctccatcgcc atgttagtcg ggttgatggc aatggctgcg ctgccgccgc
    33421 tgaacgggtt tgccggggaa tgggttatct atcaatcctt tttcaaactg agcaatagtg
    33481 gcgcgtttgt tgcccgtctg ctggggccgc tgctcgctgt gggtctggca attaccggtg
    33541 cgctggcggt gatgtgtatg gcgaaagtct atggcgtcac gttcctcggc gcgccgcgta
    33601 ccaaagaagc cgaaaacgcc acctgtgcgc cgctcctgat gagcgtaagc gtagtggcac
    33661 tggcgatttg ctg
//
LOCUS       DADLTD010000019        33554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLTD010000019 DADLTD010000000
VERSION     DADLTD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.019"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(457..2148)
                     /gene="fruA"
                     /locus_tag="JN701_000730"
     CDS             complement(457..2148)
                     /gene="fruA"
                     /locus_tag="JN701_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY7363262.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="JN701_000731"
     CDS             complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="JN701_000731"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY7363263.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3103..4233)
                     /locus_tag="JN701_000732"
     CDS             complement(3103..4233)
                     /locus_tag="JN701_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY7363264.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4601..5782
                     /gene="setB"
                     /locus_tag="JN701_000733"
     CDS             4601..5782
                     /gene="setB"
                     /locus_tag="JN701_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY7363265.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5779..6033)
                     /locus_tag="JN701_000734"
     CDS             complement(5779..6033)
                     /locus_tag="JN701_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY7363266.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6188..6760
                     /gene="yeiP"
                     /locus_tag="JN701_000735"
     CDS             6188..6760
                     /gene="yeiP"
                     /locus_tag="JN701_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY7363267.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6983..8449
                     /locus_tag="JN701_000736"
     CDS             6983..8449
                     /locus_tag="JN701_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7363268.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAVTDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8567..9553
                     /locus_tag="JN701_000737"
     CDS             8567..9553
                     /locus_tag="JN701_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY7363269.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9592..10305
                     /gene="lpxT"
                     /locus_tag="JN701_000738"
     CDS             9592..10305
                     /gene="lpxT"
                     /locus_tag="JN701_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY7363270.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10717..11283
                     /gene="mepS"
                     /locus_tag="JN701_000739"
     CDS             10717..11283
                     /gene="mepS"
                     /locus_tag="JN701_000739"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY7363271.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11464..13020
                     /locus_tag="JN701_000740"
     CDS             11464..13020
                     /locus_tag="JN701_000740"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7363272.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13102..14916
                     /locus_tag="JN701_000741"
     CDS             13102..14916
                     /locus_tag="JN701_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7363273.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14917..16011
                     /locus_tag="JN701_000742"
     CDS             14917..16011
                     /locus_tag="JN701_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY7363274.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16011..17036
                     /locus_tag="JN701_000743"
     CDS             16011..17036
                     /locus_tag="JN701_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7363275.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17038..18627
                     /gene="yejF"
                     /locus_tag="JN701_000744"
     CDS             17038..18627
                     /gene="yejF"
                     /locus_tag="JN701_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY7363276.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18631..18975)
                     /locus_tag="JN701_000745"
     CDS             complement(18631..18975)
                     /locus_tag="JN701_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363277.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="JN701_000746"
                     /pseudo
     CDS             complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="JN701_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="JN701_000747"
     CDS             complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="JN701_000747"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY7363278.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21363..23123
                     /locus_tag="JN701_000748"
     CDS             21363..23123
                     /locus_tag="JN701_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY7363279.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTDSVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23248..23532
                     /gene="rplY"
                     /locus_tag="JN701_000749"
     CDS             23248..23532
                     /gene="rplY"
                     /locus_tag="JN701_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY7363280.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="JN701_000750"
     CDS             complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="JN701_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY7363281.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24860..25087
                     /locus_tag="JN701_000751"
     CDS             24860..25087
                     /locus_tag="JN701_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY7363282.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25107..26867
                     /gene="pbgA"
                     /locus_tag="JN701_000752"
     CDS             25107..26867
                     /gene="pbgA"
                     /locus_tag="JN701_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY7363283.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26942..27018
                     /locus_tag="JN701_000753"
     tRNA            26942..27018
                     /locus_tag="JN701_000753"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26976..26978,aa:Pro,seq:ggg)
     gene            27237..28478
                     /locus_tag="JN701_000754"
     CDS             27237..28478
                     /locus_tag="JN701_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7363284.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28577..28807
                     /locus_tag="JN701_000755"
     CDS             28577..28807
                     /locus_tag="JN701_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363285.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            28974..29180
                     /locus_tag="JN701_000756"
     CDS             28974..29180
                     /locus_tag="JN701_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAY7363286.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29195..29860
                     /locus_tag="JN701_000757"
     CDS             29195..29860
                     /locus_tag="JN701_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363287.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29886..30086
                     /locus_tag="JN701_000758"
     CDS             29886..30086
                     /locus_tag="JN701_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY7363288.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30076..30297
                     /locus_tag="JN701_000759"
     CDS             30076..30297
                     /locus_tag="JN701_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363289.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30290..30523
                     /locus_tag="JN701_000760"
     CDS             30290..30523
                     /locus_tag="JN701_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363290.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30516..30749
                     /locus_tag="JN701_000761"
     CDS             30516..30749
                     /locus_tag="JN701_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363291.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30755..31054
                     /locus_tag="JN701_000762"
     CDS             30755..31054
                     /locus_tag="JN701_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7363292.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVIPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            31051..32451
                     /locus_tag="JN701_000763"
     CDS             31051..32451
                     /locus_tag="JN701_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7363293.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32654..32905
                     /locus_tag="JN701_000764"
     CDS             32654..32905
                     /locus_tag="JN701_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363294.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32902..33312
                     /locus_tag="JN701_000765"
     CDS             32902..33312
                     /locus_tag="JN701_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY7363295.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33323..>33554
                     /locus_tag="JN701_000766"
     CDS             33323..>33554
                     /locus_tag="JN701_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7363296.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGID"
BASE COUNT         8338 a         8582 c         8488 g         8146 t
ORIGIN      
        1 agcctataat tacgacataa tccttttcgc gcatagcaca tcctctggta aaaaattacc
       61 aaccctgtca atccacaaga atatagatac tgcaaagacg gtttaaacat gtgttcatat
      121 ttaaacatgt gctcatagca tgcatgtttt acgtaaagta aattaccgcc agagaggcaa
      181 aatgggcaga tttgagcagg ctcacaaaat gcgattaaat gcctgaatta caaaagcgag
      241 gaatttgtga cgggatgcac agaacgggaa caataaaaaa ggcagaaaat aatctgccct
      301 tgaggaaata accttagtgt ttgatgaacg tttcaagttt ctactgaaag ataaaaccat
      361 ttaaattcat aaaattacat tcacaaaacg gtcattctct ttgcgctcat caaatgttac
      421 agggcaggaa atttctgccc tgtaacacac cttttattac gctgctttcg ctactgcgtc
      481 cacttccgga cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat
      541 tgctaccagg taacccagta ccggcgtaat agcgccaggg atcagcagaa caaacagacc
      601 accgtgcggt gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc
      661 acccacgata cagcacggca gcacacgcat cggatcacga gcagcaaacg gaattgcacc
      721 ttccgaaatg aagcacagtc ccaataccag agcggctttg ccaccttcct gctgcgcttt
      781 gtcgaatttg cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc
      841 tgccgccata atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa
      901 tgcgtacgct gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat
      961 cgcccccagc agaaccgcat tcgcagtccc catggtctgc agccagtgag tcagcccttc
     1021 gagaatgcca gcaactggtt taccgatcag gtagatcatc gccagaccga ccaccagact
     1081 ggaaattagc ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcag
     1141 ttgcgtactg attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat
     1201 aaagccagaa ccggtgctga ccgccagcat accgccaatc agacccggcg tgaggcccgg
     1261 acgatcggca atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc
     1321 tgaaccaccg ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc
     1381 gataccaaaa gcaaaagaaa gcgcgataca cagaccacct gcgacgacca tcggcagcat
     1441 ataagagacg cctgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt
     1501 ggtcgccgtt tgagctttgc ccgccggttc atacggcgtt gcttcagcaa ccgctttatc
     1561 cagttcctgc gcggttttct tcagcgccag accggtagag gtacgataca tcggtttacc
     1621 agcaaatttc gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc
     1681 gacttcttca ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac
     1741 ccaccagcca cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc
     1801 tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg
     1861 tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa
     1921 cagctcaggg tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag
     1981 cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt
     2041 gtcgatgatt tccagttttg cttttcgcgc caccgcgccc agcagggtct tcgccatata
     2101 ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct
     2161 gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat
     2221 cggtaatacc cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca
     2281 gtgtgtgttc actggattca cgcatcagca agccataaat caggccacca accatagaat
     2341 cccctgcgcc aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg
     2401 aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt
     2461 gttcacgtag cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg
     2521 cccaaatttc caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg
     2581 ctactaacgc ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag
     2641 tcatccagtc ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga
     2701 ccatatcgaa ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcgg
     2761 gggtgacttc aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct
     2821 taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc
     2881 tgaacagttg ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat
     2941 caattcccag gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac
     3001 cggtggtttt caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt
     3061 cataagccgg attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag
     3121 accagcagcg atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc
     3181 ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg
     3241 tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg
     3301 tttaatggta ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac
     3361 cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag
     3421 caacgttgcc gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc
     3481 gagacgctta agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc
     3541 tgccgtttcg ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg
     3601 cagattgcct tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc
     3661 attgatggct ttggtgacga aagtggcgtc aactgccccc gcctctttca gacgcgcagc
     3721 gttcagcgcc tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt
     3781 gtcgagcttc agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc
     3841 tgttgttgct gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg
     3901 gcgtagcagg cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac
     3961 ctgaccgtca ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag
     4021 cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt
     4081 tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc
     4141 ctgcaccagc gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc
     4201 cggatggatg tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac
     4261 gattcagcct ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg
     4321 ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt
     4381 tatgagcaaa agtttgatgt gctgcacatt tttttgtcat ttttcctaat tgctgatggg
     4441 aaaatccgtt atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa
     4501 tggcattttg attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca
     4561 gcgtaaactc cgcgtcttcc tcttccagtg atagacctgc atgcataact cccccgcagt
     4621 ctccagcgcg aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac
     4681 cggtattgcg ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca
     4741 tgcccgtccg gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt
     4801 aagtcagttt ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt
     4861 ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt
     4921 tttgctattc gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt
     4981 tgcccttgcc cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt
     5041 tttacgcgct caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc
     5101 gatgggtttc agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg
     5161 cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac
     5221 ggttgaagcg ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt
     5281 gatgtggggc tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact
     5341 gcatcttccc gagaaactgg ccggtgtgat gatggggacc gccgccgggc tggaaatccc
     5401 gacgatgttg attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt
     5461 tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgccat
     5521 tctgttgggc ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat
     5581 gctctatttt caggatttga tgcccggtca ggcaggttca gccaccacgc tctataccaa
     5641 cacgtcgcgc gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg
     5701 gaattatcac gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt
     5761 actgcggact aaagatgttt aagacgcggt cagcatttcc agatcgataa gccaggtcat
     5821 ggcctgctga cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt
     5881 cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg
     5941 cgtattggcg ggcttgccat ctggcatacc gggaataggg ctgaaaattg aaggggcagt
     6001 gcaacacgcc ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg
     6061 gattgatgag cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg
     6121 cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa
     6181 tttttcgatg ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa
     6241 actgctgctg gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct
     6301 gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg
     6361 tgatgatatc gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga
     6421 tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga
     6481 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
     6541 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
     6601 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
     6661 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
     6721 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
     6781 gggatagctc aggctgcccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
     6841 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
     6901 taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta
     6961 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
     7021 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
     7081 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
     7141 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
     7201 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
     7261 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
     7321 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
     7381 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
     7441 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
     7501 tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
     7561 tgtggtgaaa aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac tcgccgggtg
     7621 gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgtaac
     7681 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
     7741 ctgcgaaccg tttatccagt gggtggtgga agataacttc gtcgctgggc gtcctgcctg
     7801 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
     7861 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctttcag gattcgccca
     7921 tatcagtgat tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat taatgctgaa
     7981 tgagcaagcg ccgacactac aaattaaaga tgtcgattta acacaatatg cggacaagtt
     8041 aattgctcgt tttgctaatc cggcgctgaa acataagacc tggcaaatcg caatgaatgg
     8101 tagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgcgaaac
     8161 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
     8221 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
     8281 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
     8341 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
     8401 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
     8461 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
     8521 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
     8581 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
     8641 taaagatccc aacgaaaaat ggacggtact ggttaatgaa tttggggaag tcggaattga
     8701 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
     8761 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
     8821 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
     8881 aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc
     8941 acgcttgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
     9001 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
     9061 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
     9121 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
     9181 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
     9241 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
     9301 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
     9361 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
     9421 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
     9481 ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc
     9541 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
     9601 aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat
     9661 attcccgtta atcatggatt atggttgccg attgatgcgg atatttttta tttctttaat
     9721 cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgaccaa caatcgcgct
     9781 ttcgacggtt gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa
     9841 aacgccccag gcagacgacg tatcgtgatt attggtctgg taatgctatt aactgcagtg
     9901 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgacg
     9961 tttactaata ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga tgcctcacga
    10021 gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
    10081 tatttcggta aagttgcagg tcttatcgcc cttattattt ttgtggtttt tgcgtttccc
    10141 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
    10201 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
    10261 gacaaatcac taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
    10321 atcatcaggt ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct
    10381 atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg
    10441 tactttaatt gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc
    10501 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg
    10561 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
    10621 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
    10681 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
    10741 agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
    10801 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
    10861 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
    10921 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
    10981 aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta
    11041 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
    11101 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
    11161 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
    11221 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgagttct cagccgcagc
    11281 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
    11341 taggtagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
    11401 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
    11461 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
    11521 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
    11581 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
    11641 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
    11701 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
    11761 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
    11821 ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
    11881 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
    11941 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
    12001 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
    12061 cgtttgatga acgttaatga attaaccgac atgcctgtcc gtgaaactaa aattgcgggc
    12121 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
    12181 tttttactgg gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc
    12241 gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg
    12301 gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta
    12361 cgctggcggc atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
    12421 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
    12481 gcagaattag aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac
    12541 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
    12601 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
    12661 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
    12721 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
    12781 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
    12841 ctgacgctgg cgaaacgcct caatatgctg acggttgctg aagggggcga aacgccggaa
    12901 caggcgcgat ggctaagcga acccggcgtt aatttcatgc aaggctactg gattagtcgc
    12961 ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa
    13021 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
    13081 gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat cgccctgttc
    13141 accttcggtg cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa
    13201 ccccggtacg catttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
    13261 gggcagataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc
    13321 ggcaacccgg gcgcacgcac cgagcagttg tacgacacgc tatttacgac ttccgatgac
    13381 gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc
    13441 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc
    13501 cgcgatgtag agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg
    13561 gtctacaaag gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct
    13621 aaacccggca aagaagatat gctgagtctg ttttcgctgc cggtatttcc agaaaagtac
    13681 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
    13741 cgcgttacgt cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa agattactgg
    13801 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
    13861 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
    13921 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
    13981 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
    14041 atccaacgtc cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac
    14101 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
    14161 cagaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
    14221 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
    14281 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acacacttct caacgaagcg
    14341 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca actcagcttt
    14401 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg
    14461 caacggctgg gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc
    14521 atgcgcagtc gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt
    14581 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
    14641 gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa
    14701 gaaaaattat tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
    14761 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccacccc
    14821 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
    14881 gccaaactgc cgtcagccag gcaacaggga gagtagatgg gcgcttacct gattcgccgt
    14941 ctgttgctgg tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa
    15001 attgcgcctg gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga
    15061 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
    15121 aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag
    15181 atcactcatc gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg
    15241 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
    15301 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
    15361 ctggtgtcga ttccgttagg cattcgcaaa gctgttcata atgggagccg ctttgacgtc
    15421 tggagtagcg catttatcat tatcggctac gccattccgg cctttttgtt tgccatcctg
    15481 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
    15541 tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc
    15601 acgctgccgg tgctggcgac ggtgattggt ggctttgcgg cgctgaccat actgacaaaa
    15661 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
    15721 agtgaaaaaa atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc
    15781 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
    15841 ttttcactca atggtctggg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
    15901 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
    15961 gatatcagct atacgctggt tgatccgcgt attgattttg agggacgtta atgtcgcgac
    16021 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
    16081 cgttatggat tttcctcgtc ttgtttggtt tgagtttgtg ttctgagctt atcgccaacg
    16141 ataaaccatt gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca
    16201 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
    16261 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
    16321 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
    16381 gaacggatgc caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
    16441 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    16501 tacaaggcta ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt
    16561 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    16621 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    16681 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcgcaggcg cttggcgtca
    16741 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    16801 ttttaccgtt tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct
    16861 tcggtctgcc gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc
    16921 ttcaggctcc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    16981 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    17041 acgcaaactc tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta
    17101 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    17161 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    17221 ccgccggttg aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc
    17281 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg
    17341 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    17401 caccgcggga tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt
    17461 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    17521 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    17581 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    17641 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    17701 aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc
    17761 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    17821 tcaggcgatc cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    17881 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    17941 gtgaaaaaca tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc
    18001 ggttccggga aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc
    18061 atcatctttg acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt
    18121 catcgcattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    18181 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    18241 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    18301 tatccggcgg agttctctgg tggtcagcga caacgtattg caattgccag ggcattaatt
    18361 cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    18421 gcgcaaatat tgacgctatt gaaatcattg caacaaaagc tgcaactggc ctatttgttt
    18481 atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    18541 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat
    18601 acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    18661 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    18721 tacaaacagg cacggctcac cttcacactc cagcaccgaa cacggaactt cgatgtcgct
    18781 taacgcctgg cttagtttgt acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    18841 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt
    18901 cgaacctgcg aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag
    18961 ttgtaatgaa gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca
    19021 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    19081 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    19141 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    19201 acataaattg attttacata aaataaacat atatcggggg aatgatagat ttgtggttga
    19261 catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc
    19321 ggtcgactgg cgtacagaca gaagagaatg gagctgcttg cgcagaatgc aattgaccaa
    19381 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    19441 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    19501 tcaagaatga ccgccatcgc attggatgac accatcgaca cacagcccac aaacgccgca
    19561 acgccaacca ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa
    19621 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cgaacgaagc
    19681 ggctgttaaa gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa
    19741 agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg
    19801 agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    19861 ggaacagcgc cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg cgacgctccg
    19921 gtggtaaggt ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg
    19981 cgaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg
    20041 gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact
    20101 cttctttcgg gtagatatca cgcatcaaag cgttgataac catgctggcc gcagccgcag
    20161 ccagcccgtg gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg
    20221 ccaccgcggc ggcggcaaac accagcgtac cgccgagcac caccggctta cgcccgaagc
    20281 tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga
    20341 gggtcatctg cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg
    20401 gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa
    20461 tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt
    20521 agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag
    20581 catccagcgt aataccgcca atacgttcac gatgcagctc aaccacgtgg ttacccacgg
    20641 cggcgaacat gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg
    20701 ttggggtaat cacttccagc accgcaggct tagtgagatc tttttcgtta tgcagctgca
    20761 cgcctttagc aaattgctct gccgtatcgt cagctacagg tgattccagt gtcaccagat
    20821 aggtcttctc gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatcag
    20881 tcatcagcac cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc agtttccagg
    20941 ctaccggttc atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa
    21001 catagccctg aggcttattg agcatgaagt aacgtggacc atgttgctgc gccagcgggt
    21061 tgccatcgta agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cgaacaattt
    21121 cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc
    21181 cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg
    21241 aggtcgggca ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac
    21301 atccctcaag ggaaaaagat ccatggcata ctattagcag aataatctac ctacgcgaga
    21361 ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt
    21421 ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg
    21481 tgattgccga actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca cacgtcaaag
    21541 aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg
    21601 ccgctgggtt aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg
    21661 tcgctcgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgcc
    21721 accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgactaaag
    21781 tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggct
    21841 ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg
    21901 actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg acgccgccag
    21961 aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg
    22021 ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc attacgccac
    22081 atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg attttcgccg
    22141 ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga
    22201 ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc
    22261 gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg
    22321 atcttatcgc cattctgcgt cctaccgact cagtgagtct ttaccaacaa attgtcgggc
    22381 gcggtctgcg tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc
    22441 ctcacgacct ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgtcccgg
    22501 tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg
    22561 acgggacatt gattgaacac tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg
    22621 gccatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg
    22681 aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg
    22741 atatgttaaa agcggcgcta cgactgaaag acgcgctggt attacgctgt agcggcatgt
    22801 ctttacagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag
    22861 acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg
    22921 agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg
    22981 ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc gtcgcccgca
    23041 tgaaaggtca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc
    23101 gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata
    23161 gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt
    23221 agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag
    23281 cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac
    23341 ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa
    23401 gctaaagctg aattctacag cgaagttctg accatcgttg ttgacggtaa agaaatcaaa
    23461 gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc
    23521 gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat
    23581 ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg
    23641 ccgcatctgg cattcagaat acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa
    23701 ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg ctggatccca
    23761 gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc
    23821 ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct cttccagttc
    23881 gtagcctttt tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg ccagctcttt
    23941 cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata
    24001 aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc
    24061 atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc
    24121 gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag
    24181 atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc
    24241 cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat
    24301 actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg
    24361 gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg
    24421 cccggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag
    24481 cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata
    24541 gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc
    24601 gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg
    24661 gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc
    24721 ggttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta acgagaatga
    24781 tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc
    24841 aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca
    24901 attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct
    24961 gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg
    25021 ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc
    25081 gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag
    25141 tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg
    25201 tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt
    25261 attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga
    25321 tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca
    25381 ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc
    25441 atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg
    25501 cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt
    25561 ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc
    25621 cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg
    25681 atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga
    25741 tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc
    25801 gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta agcgaactgc
    25861 gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact
    25921 actcacgctt cgagaagcag atgcctgcac tggcaggttt tgctgagcaa aatatttcgt
    25981 tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg
    26041 gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacccctgcg gcgttaatta
    26101 ctgcgcttaa tcagcaaggc tatcagctgg gattattctc gtcagatggc tttaccagtc
    26161 cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgt ccccaatccg
    26221 acgagcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa gaagataacc
    26281 gctggttctc gtgggtctct ttcaatggca ctaacattga cgacagcaat cagcaggcat
    26341 ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca
    26401 atgcactgct tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg
    26461 gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc
    26521 cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata
    26581 ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat
    26641 attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtc actgcagcgg
    26701 ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta
    26761 aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt
    26821 tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc
    26881 agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag
    26941 tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa
    27001 atcctctcgt gccgaccaaa tcgcccaccg gataccaccg gaattaacag tttttagaca
    27061 cgaaaagccc gctaatcctt gattagcggg tttttttatg gtttttagtc accggacatc
    27121 accggattta taagctatca gccggatttt ttagttatac gcttagttat acgggcttgc
    27181 gtataactaa agccgtataa ctaaaaccct gttttttcgt gtggaatcat atacatatgg
    27241 cgagaaaaac acccccatta accacggtgc agatcaaagc agccagacca gcggaaaagg
    27301 aatacaccct acaggacggc ggagggcttt ttctcctggt caaaccgtcc ggatcaaaac
    27361 tctggcgatt ttcctactac cggccatcgg acaaaaaaag aatattgctg agttttggat
    27421 cgcttgatga tgtttccctg gctgatgcca gaaaacgccg tagcgagtac agagcgttaa
    27481 tcagtgccgg aactgacccg cagggccatg agaagaaaaa acgagaggca gaggcccgaa
    27541 gacaagggaa cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa atcagccaga
    27601 atctggcccc caacacgatt aaagacatct ggcgttcgct ggataaatat gtattcccgt
    27661 tcatcggcaa cacgccaata gataccctca ccgcccgaag gttcgttgaa gtgcttacac
    27721 ccatcaagga gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc gttaatgagg
    27781 taatggatta cgccgccaac agtgggctga ttgatgccaa tccggctatg aatgtgcgta
    27841 aggcgtttcc ctcacctgta aaaaaacata tgccaacaat ccgccccgaa cagctgccgg
    27901 agcttatgca ggctttatca gtatcggcaa cagaacggca gaccagatta ctgattgaat
    27961 ggcagttact gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg gatgaaatca
    28021 acctggacgc gaagcaatgg acgatacctg ccggacgcat gaagatgcgc agggatcacg
    28081 ttatcccgct ttccggtcag gctatggcgg tgctggaggc catgaaacca atcagccacc
    28141 accgcaatta cgttttccca agtctgaaag acccacagca gccgatgaac agccagacag
    28201 ctaacgcagc attgcggcgt atgggattcg ctggcgtgct ggtgtctcat ggattacgcg
    28261 ccatattcag cacagcagcg aacgaggaag gattcgagcc ggacgtaata gaggcggcac
    28321 ttgcccacgt cgacaccaac gaagttagac gggcatacaa ccggagcaac tacatagaaa
    28381 aacgcatcgt gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg acgggcgtaa
    28441 ccctcgccag tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa ccagtaaaaa
    28501 ctacgaaaac catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca aggccgtgaa
    28561 acatgaaacc gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc tgcaactggc
    28621 cctgatggtt cagcatgaat tttggtcaac ctacgatccg gaggacagaa cgacggcccc
    28681 atcaaaaaaa caggtagtag attttctggt atcccgtggc gcttccagaa atctggcggt
    28741 aagtattgat aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc ccaaaaaatg
    28801 gcggtaacaa tcctggaagc ggcagaaatg ccgctttttt tataattcca tttcaaatca
    28861 tcaatataaa aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg
    28921 atgatttacc gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag
    28981 caatcagaaa aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca
    29041 gcacgctgca aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac
    29101 gccgtcgcgg atggtatgag gatgatgtgt tggcctggca gaaagaacgc gaacaggaag
    29161 cacgcggaac ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc
    29221 gatatggagc cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat atgatcccaa
    29281 acataagcac ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc
    29341 agttcgttgc catacctgca atgagcgctt ttccctatac tctttaagga attgctcaag
    29401 ggcaaaagca catggtgcga atctttctga ttcatgctct atctttctgc gccgtctttt
    29461 ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc
    29521 aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc
    29581 gcgccgcttt agttcagtgc gccccaactc tttaagccag ttggctaggc ttatgccgtc
    29641 gctctgtgct tctttgtcga attgctcttt tagctctgga gaaattcgca ttctgaattg
    29701 tggggattgc ccgtcccctt tagggctttt atcgcgtttg attattgaca agtggtcacc
    29761 tattgaatta gcctttccat tgttaggtgg ccaccttaac acaagagcac ttaaaaaagc
    29821 aaagccccgc aagtgtcatt accactcgca gggcttctaa ccaacaacgt aaactaggag
    29881 ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt
    29941 ggcgttttct ggccctgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg
    30001 aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc
    30061 gtcaggggga aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact
    30121 acgctgatgc acgcgcttat tgatattgaa tgcactgcag agcttgcgca gggagaagaa
    30181 cagaaagacc ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcgctg
    30241 aatgacgcta aaaatattct tgttgctgat tgtgaaaatg gggggggtta tgcgtgatga
    30301 tcgttttaat tccctgaaac aggaatttcc cggcgttcct gatgatgcgg ctgatgcgct
    30361 ttcgtcaatg ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa
    30421 atcaacgggg cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc
    30481 gcgttacaga agaaaatttc ctgaggatgt aagccatgcg tgatatttac cacgaaacaa
    30541 tagaccgcgc atttcttgca ctttctcaca gtgaaaacat gctggaaata ttgcgcatat
    30601 ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat
    30661 taataacgct tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca
    30721 gatataaaga acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac
    30781 tgccagtggc aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg
    30841 cggcacggta atgattaagg gcgtggaaga acggcgtgta acctaccgcc gtgaaggtta
    30901 cgaatatgat tgcgtgatac ctgtttatca gttccggcgt gatttttctc tggtacagac
    30961 cgcgccgcat aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa
    31021 aaccatgatt aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag
    31081 ccttacgaca agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgcg
    31141 aaacagtggc aggaacagga cgggcgactg gctggcgata atgtgcctcc cgttgtgctg
    31201 gctgatgatc aactggatga actggcagac ctgagaatca tcgacgaggg gcgctattgt
    31261 gtccggctgt acaaggcagg ccacatcagg ccatcaaata ttaatgccat tgcgcacaag
    31321 ctggcggcgg cgggtgtaac tgatgcgaat tattaccccg aagggatgca cagccatatg
    31381 cgggagaact ggcgcgaata cctggaacgg gtgcgcggga aagagccggt ggaagaaaaa
    31441 aaccaccagc gaaaaaccac gctaccgatg agcgttggat ctaccggata cgacacgcaa
    31501 ctggattacg tggttaaggg gattattccg gcggtatcgc tatgcagcat atacggggct
    31561 agcgggtcct ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc cactggtcgc
    31621 cagtggggag gccgcagggt tgcacatggt gcggttctct atgtggttgg tgaaggcggt
    31681 atcggcgttc cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca ggtgaaaaat
    31741 ctgtatctgg taaaccgccc catctttccg gctgccccgc ttgatgttga tgaaatggtt
    31801 atcgctgccc gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat tattctggat
    31861 acgctggcgc gttgctttgg tgggaatgat gaaaatgatt cccgtgatat gggggcgttt
    31921 atccgtggtt gtgacgaact gaaacgacgc acaggggcca cggtgctggt ggttcaccat
    31981 tccggcaagg atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc ttcgctggat
    32041 gctgaatacc ggatacgcag ggaggacgca ggaagcgaag cgctggttat ctcatgcacc
    32101 aaaatgaagg acgcggagga actcaaagaa gccgcatatg acttacgcgt ggtggagctt
    32161 tttaccgacg ctgacggtga attaatcacg tcgctggtgg tggtggatga tccgcgccct
    32221 cctgttgaac tggagcgcat cgaggaggca gggaacaaga cggaaaacca taccgcgcta
    32281 tgggggtgca tccgttcacg cacacagaac ggcgacaagt gcacgatccc gctgttacgt
    32341 gatgacatga aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct gtacaagctg
    32401 gaaaaagatg gggttattag tatcgatggg gatgatgtag cgccgctata aaagtgagga
    32461 gcaaaagcga gggggataga aagagggcca aaattagccc gctctccctc acttttcgac
    32521 ctgtatacat cctcaaaagt gaggggtaaa aaaaaatact tataaaacat acacatagaa
    32581 aaaccgaaaa tcccaactgc gacgaagtga gacgcttgaa aaagtgaggc gaaaaagtga
    32641 gaggttgcga gaaatgaccc aaaaacgcag agacagaaca gagccaaaat ataaagcgtt
    32701 agacatgact gagcacacct taaaggtggc aatcagaacg atagaccgcc acacgcggga
    32761 aggatacgcg aaggaacatc ccgacctgat aagcgcattc atgaccacgg cggcggcaaa
    32821 ctttgccacg ctgacagaac gggagattgc cgaagcggaa caggtaacaa ccatcaacgt
    32881 taaaaccgga gaggtggaat tatgacggca cagatagccg cttacgggcg gctggtggac
    32941 gacccgcagg taaaacagac cagcaagggc acaccgatga cgctggcgcg tatggcggta
    33001 tctttgccat gcagccaggc acaggacgga caggcgacgt tatggttatc ggtcatcgca
    33061 tttggcaaac aggccgactt cctggctaaa catcaaaaag gcgatgttgc cagcgtatcc
    33121 ggcacgatgc aggtaagcca gtggaccgga cagaacggag aaacgcggca gggctggcag
    33181 gttatcgcag acagcgtaat cagtgcccgc gcggcacgtc caggcgggaa cagacgcaaa
    33241 accacaggca cacagggtaa tcagccacca gcgggaggcg atgaccctta cggtgatgat
    33301 atttcgttct gagggggtga cgatggtaca tgaccgcata gcggaggaac tggaggcgaa
    33361 aggcttttac cggagggcgg cggcgcgatg gggtgaagtc atgcagctgg tggagacaga
    33421 caaggaacgg catcacatca cgatgcgacg gctggaatgt tcaaggaagg cacagagggc
    33481 accggagccg ccggataatt tcggagacct gaaaaaggca gtcgatcgca cttatgccga
    33541 aatgggtata gatg
//
LOCUS       DADLTD010000020        33375 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLTD010000020 DADLTD010000000
VERSION     DADLTD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.020"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="JN701_000767"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="JN701_000767"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY7363297.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2991..3920)
                     /locus_tag="JN701_000768"
     CDS             complement(2991..3920)
                     /locus_tag="JN701_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY7363298.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4137..4373)
                     /locus_tag="JN701_000769"
     CDS             complement(4137..4373)
                     /locus_tag="JN701_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7363299.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4377..6278)
                     /gene="ipaA"
                     /locus_tag="JN701_000770"
     CDS             complement(4377..6278)
                     /gene="ipaA"
                     /locus_tag="JN701_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY7363300.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGLTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6287..7285)
                     /gene="ipaD"
                     /locus_tag="JN701_000771"
     CDS             complement(6287..7285)
                     /gene="ipaD"
                     /locus_tag="JN701_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY7363301.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7336..8427)
                     /gene="ipaC"
                     /locus_tag="JN701_000772"
     CDS             complement(7336..8427)
                     /gene="ipaC"
                     /locus_tag="JN701_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY7363302.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(8447..10189)
                     /gene="ipaB"
                     /locus_tag="JN701_000773"
     CDS             complement(8447..10189)
                     /gene="ipaB"
                     /locus_tag="JN701_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY7363303.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10195..10662)
                     /gene="ipgC"
                     /locus_tag="JN701_000774"
     CDS             complement(10195..10662)
                     /gene="ipgC"
                     /locus_tag="JN701_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY7363304.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB"
                     /locus_tag="JN701_000775"
     CDS             complement(10720..11346)
                     /gene="ipgB"
                     /locus_tag="JN701_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY7363305.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="JN701_000776"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="JN701_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY7363306.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="JN701_000777"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="JN701_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY7363307.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="JN701_000778"
     CDS             13562..15178
                     /locus_tag="JN701_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY7363308.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="JN701_000779"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="JN701_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY7363309.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="JN701_000780"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="JN701_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY7363310.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16017..17132
                     /gene="mxiG"
                     /locus_tag="JN701_000781"
     CDS             16017..17132
                     /gene="mxiG"
                     /locus_tag="JN701_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY7363311.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17140..17391
                     /locus_tag="JN701_000782"
     CDS             17140..17391
                     /locus_tag="JN701_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HAY7363312.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17404..17697
                     /gene="mxiI"
                     /locus_tag="JN701_000783"
     CDS             17404..17697
                     /gene="mxiI"
                     /locus_tag="JN701_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY7363313.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17703..18428
                     /locus_tag="JN701_000784"
     CDS             17703..18428
                     /locus_tag="JN701_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY7363314.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18425..18952
                     /locus_tag="JN701_000785"
     CDS             18425..18952
                     /locus_tag="JN701_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY7363315.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18894..19589
                     /locus_tag="JN701_000786"
     CDS             18894..19589
                     /locus_tag="JN701_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY7363316.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19582..19989
                     /locus_tag="JN701_000787"
     CDS             19582..19989
                     /locus_tag="JN701_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY7363317.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19973..20401
                     /gene="mxiM"
                     /locus_tag="JN701_000788"
     CDS             19973..20401
                     /gene="mxiM"
                     /locus_tag="JN701_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY7363318.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20531..21163
                     /gene="mxiE"
                     /locus_tag="JN701_000789"
     CDS             20531..21163
                     /gene="mxiE"
                     /locus_tag="JN701_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY7363319.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21150..22850
                     /gene="mxiD"
                     /locus_tag="JN701_000790"
     CDS             21150..22850
                     /gene="mxiD"
                     /locus_tag="JN701_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY7363320.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22869..23936
                     /gene="mxiC"
                     /locus_tag="JN701_000791"
     CDS             22869..23936
                     /gene="mxiC"
                     /locus_tag="JN701_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY7363321.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23949..26009
                     /gene="mxiA"
                     /locus_tag="JN701_000792"
                     /gene_synonym="virH"
     CDS             23949..26009
                     /gene="mxiA"
                     /locus_tag="JN701_000792"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY7363322.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26022..26423
                     /gene="spaK"
                     /locus_tag="JN701_000793"
     CDS             26022..26423
                     /gene="spaK"
                     /locus_tag="JN701_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY7363323.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26427..27719
                     /locus_tag="JN701_000794"
     CDS             26427..27719
                     /locus_tag="JN701_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY7363324.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            27812..28150
                     /gene="spaM"
                     /locus_tag="JN701_000795"
     CDS             27812..28150
                     /gene="spaM"
                     /locus_tag="JN701_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY7363325.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28137..29015
                     /gene="spa32"
                     /locus_tag="JN701_000796"
     CDS             28137..29015
                     /gene="spa32"
                     /locus_tag="JN701_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY7363326.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29009..29890
                     /gene="spaO"
                     /locus_tag="JN701_000797"
     CDS             29009..29890
                     /gene="spaO"
                     /locus_tag="JN701_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY7363327.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29895..30545
                     /gene="spaP"
                     /locus_tag="JN701_000798"
     CDS             29895..30545
                     /gene="spaP"
                     /locus_tag="JN701_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY7363328.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30560..30820
                     /gene="spaQ"
                     /locus_tag="JN701_000799"
     CDS             30560..30820
                     /gene="spaQ"
                     /locus_tag="JN701_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY7363329.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30820..31590
                     /gene="spaR"
                     /locus_tag="JN701_000800"
     CDS             30820..31590
                     /gene="spaR"
                     /locus_tag="JN701_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY7363330.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31597..32625
                     /gene="spaS"
                     /locus_tag="JN701_000801"
     CDS             31597..32625
                     /gene="spaS"
                     /locus_tag="JN701_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY7363331.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32638..32880
                     /locus_tag="JN701_000802"
     CDS             32638..32880
                     /locus_tag="JN701_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363332.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10437 a         4826 c         6660 g        11452 t
ORIGIN      
        1 ttgttgactg cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaatcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg cggtattaat tgctgatgct
     1081 gatggacatt aactcttaat agaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg ggggcataac
     2101 aaatgatatt actgagtaca aatttagcta tattgggcgg attagttgtg tgggaagacg
     2161 aaatgtttct cttgaaataa gtgaactccc tcaaggaatg attgtgtgct taaatattta
     2221 agtgctggta agtttgagaa ggctgacata ttttgcagag caaacagtga ttgacttgga
     2281 tgatattttt attttagtca ctcgttgtaa ttggagtatg ctttatttta tttgaaaata
     2341 gatgtatttt atacccgcaa tttaaactgt aagccctatg aggggtatgg atattataag
     2401 ttactctttg actaagcata acttttgggg ttagaaaaat aaatctcttg ttaataatta
     2461 atatgatttt aaaatcaaac ttgtttataa ttataaaata atgtaagtcg gtcattttat
     2521 gggaagatta ggagaataaa acagtaatta tatttgagaa atctgctaat atattcttaa
     2581 agcttttttg atactgtgaa cgaagtctgt taatggtgga ttttttgttt atcaccatgt
     2641 aaaaaaattg agtagtaaaa gcgttgactg ctaatcctcg ctgtttttat tgaaacgatt
     2701 aaaaatacgc tgaatatttt cggccagtca ctcatcttca aataaaattt tgaccatcat
     2761 gccgcatccc ttagttgcac aattcatttt caatgatatg aatattacta atttcaccag
     2821 ataatgttct cgtggtcagc agggtatgag ttttcaccgt aattttaagg aaacacagtg
     2881 cgtggggatt gaataacaga tagccgacgg aattacccca ccggctgaaa gtcaagttca
     2941 acgaagattt ttattatctg aattgggcag tttacatcag tgttcgatgt ttatgaagac
     3001 gatagatggc gagaaattat atctcgaata gcttcatcta tttctctctg aactgttttg
     3061 tttattttag ataatgtaaa tattagagtt ctgtttgttt tattctcttt tctttttatg
     3121 taagtccgtt tgtctttaga aataaataac gagtcaactt ttagcgaagg gttttgcttt
     3181 ctgtttttgc ttttctttat gatgtttaaa atttcttttt tatatatgtc cgggggcaaa
     3241 tttgtatcaa ggtcttttat ttcttccttt aatattggta gtgtagaact aagagattca
     3301 ttagcctttt caagtccttt ataataattg aataatatct tgtagtcatt aaagttaagt
     3361 tctgacgcga ttggaaatag agatattatt tcctgtggaa cgcttgctgc ctgaaaggca
     3421 cgagtgactt tcgcgcgaga cagattctct tttttggcta tgtctttata ggacatccct
     3481 gatactttca gaaaattaag accaatacca agttctcgga tgctatgctc ttttgctgtt
     3541 tgtatatcgt ttgctagttt tctggcatca agagtagata tatattcttt tgaatataga
     3601 acttcaagat ctgctcctgc atatattgca gatgctcttc tacgagtgcc atccagaatt
     3661 tcaattctac catcaatctc ccttcctatt acagggaaga attgttgtag ctttatagtt
     3721 tttatgatat cggctaatga ttcttctgtt agggattctt gatcgcgtac attcgttttt
     3781 tggttgacga aggttaaatc ttggatcctt ttatgaggaa tcttggcttt gataaaggaa
     3841 actttattac cagaatggag tgtaaactct ttcttttggc cttcctttat acttaacaag
     3901 tcgttgcaca aatccaccat atcacaccct gtttattcat attgatttat attgacaaaa
     3961 gttaaatgca gtgtgagcaa gattgtatgt aactgttgat gatagtcaac ggtagtctta
     4021 aaaattacta ttttaatagc tggattgatg caatttatct ttattggata aaagaataat
     4081 attcttgtgg agctctgatg tgagagctcc atagatgaaa aaatgcttat caattactat
     4141 attgattttg tactattttt gactacagaa ataagatctc caatagtaat aatgtgttct
     4201 aatgcagatt catctatacg tagattgaac tcaaaactaa tcatatccag catttcaatc
     4261 agagaaagag aatcggcaga aagatcttca ataaatttag tttcttcaga tagtttgctg
     4321 tacgctatac catagcaaaa ggccactatt gataatattt tttcttttat catattttaa
     4381 tccttattga tattctttaa tacttttgat agggaagtgg ttacatcttt tgctgcttta
     4441 tatatattat tgttttcagc tgtaatatca ttgttatttt ttaaatcaga tatatcatct
     4501 gtaagtaatt ctgcagaggt atcgtcaatt tttgacaata ccttgctgag ggcgctagat
     4561 acttcttttg ccttttcata gatcgcatga tttttatcaa ttgtatctgc gttggtttct
     4621 gttacgtagt ttattgtatt tgcatctgta acttttaatt tgttgtttat tctgtgttct
     4681 gttgtggatt ttgtaatgtc ctttgatttt tcctgtaact ctcgttcttg tgttcctgat
     4741 ttagttccaa tgagagatat aagattactt agggagtttg ttactttttt tgaagcttca
     4801 aatatcgttt cccgtgttaa atatgtgtct ccatttcttg ataaaaaatc agtctgaagt
     4861 acttttgaat cagtgtttga gttgaattta ttgatttgcg aagctactct atgagaaaga
     4921 gaagcgctac ttttagttgt atctgatgag ttatcattac tgcttgtcag tccttccggg
     4981 tggagagggg gggtggttga agagttctgt atgttcagag gcctgtgtcc ccgagaaaga
     5041 gagttatttg tagatctttc ttgactactc gtagttaaca ggtctgaacc tattccatcc
     5101 gaattatctg gagtatggtt gaaaaccttt tctaaagctg aattatttgc aaatgtctgg
     5161 acagtatctg atatagagtg tggtagaacg gaagttactt tttgaactag tgctctttgt
     5221 ggatttagta atgagtttcc gttacgggat aatagatcgt ttgtctgaga agtaggagac
     5281 tgtgcatcat tttctgggtt ctcgtgatag ctattgtcat atactctgtt gtcaaaaact
     5341 ctattatcgt aagttctatt atcattattt atatgatagt gaattacagg ttgggatgtt
     5401 ttctcattgg ctccgggggt tggtccgcca tctggcatat cttcgggagg tgtagggagg
     5461 ccatcttgga taggatgcga atcttttata tcttctaaaa tatcattaag gacttttctc
     5521 aacagctcag gaaacacggc tactgtattt aagtctcttg acagtttacc tataccaaat
     5581 cctttactat caatagtatt tttacgttga ttgcgcaaac tatcaaaggc aagttttgcc
     5641 tgtgcgtcaa ctaaatcatc tagtttgaga gatgaaacag tttcattact taactttaat
     5701 tcagcaagaa tattattctt gataaatgat gatacttcag tattgaggag tttttgtccc
     5761 aatgataaag cttcaccaga tgtgtgttgt tgtgctaata gtgcaagtct gtgctcaatg
     5821 ttttgtcgta attgatggag tttcttattc tttgccgacc atggtccaaa ggcagaatct
     5881 tgggataatt tatcttttat aattagttcc gaatactgtg cgacaatttt ttgaagtccc
     5941 ttttcgtttt caaaattaag atcattttga tgtactaagg agtacaaaac atttgaaaat
     6001 tcgagaatct cgcttttggc atacgcattc attgagttgg aataaatgct tcttaacaca
     6061 tcttctttcc ctgaggaaga aaataaaaaa tcaagacatt tctgattaaa agacgttgca
     6121 aagttttctt tttcagacac tgatgctggt gtttttagag aagtttgcga actatggata
     6181 tcggatattt tgctttttaa ctcgctgtat tctgttgatg atggtgaagt tgccttatat
     6241 aagaatgttg gcgcttgagt attatttaca ttatgcatgg cgcacctcag aaatggagaa
     6301 aaagtttatc tgtatctgta catgagctta ttgtactact caaaaccttt actaaattat
     6361 caaaaatact attggcatta ctgtattttt gaactaaagt ttgaagatta tttttcattg
     6421 tttcatcttc ggcagagaat ccggcattcc atgcctgata ttttgcatta tctagcacaa
     6481 cctcgccatt tccacctaga ttatctaagc tttttaacat attgtctatt ggggtcatgt
     6541 ttatattgac aacatatccc ccgttttttt cagatacctt gccgattgtt ccacctaatt
     6601 ctgtaagcca tttatttgct tgttccttac taacagtatt atttgctgga tatagcggtt
     6661 tatctttata tttttccttg agctttgtca attcgtcttt aagcgatttg acttgtaatt
     6721 tcacggagtt tccgtcgtta cctccgggag agatccagcc ggcaagactg gaaagaacag
     6781 cgctaaaatc ttgatacatt tgagtatatg aactaacggc atgttcatat actttcagat
     6841 actgttcatt aatattattg attgactttg caattttatc ccacagttct ctgtgagata
     6901 tcatttcata tccatcaagc tcggcttctt tcggggctga atgtaataat tctcttgcgt
     6961 ctttattaat tggatattct ttcttggaaa gaatatccaa tagttgagcg gatttgttta
     7021 catccatgct aatctgagat gaatgtaatg ctatttcttc tagcgatgtt ttagtcaacg
     7081 ttttttgtga gagatctttc tttaatgcct gatttgttgt tctgatatta tgaagggtgt
     7141 cgttgagcat agtaagggaa cttacaggat gagaactggt cgttgttttt atatcagaat
     7201 taactgtttc ggttgatgaa ccgttggtat tgtttgggct gaatgatgag gtggaaatac
     7261 tattagtcag agttgttata ttcatggtta tttccttata ggaagagcca tatattatta
     7321 tggctcttgg atatattaag ctcgaatgtt accagcaatc tgactggctg tagaattcct
     7381 tgattggttg atgttgtcaa ttatattcaa taatttttgt attaattgat ttgtcgcttg
     7441 ggatgcttct ttagatactt gggatgcttc ctctgcttgt ttactgctgg cctgactgat
     7501 tagttgttct tcttcttcaa gagcagatgt ataacgccct cctgatgtgg atatattacc
     7561 agcaacggct gatgtctcca ttgttgcacg gccaatgttt tgtttttgct gagcagaaag
     7621 ggtattgagc tcgtaagctc ttctctgggt gtcaatttta tcctgcaaag aaatatcggg
     7681 agaactaaga gaagttttat gttcagttga cagggatata ttatctggtg ccagtttatt
     7741 tttacctaaa atttttgtgc tagagttagt ttgtggtgag gtgatatttg tatctatttg
     7801 tttatttaac cctaatttag aacctgcaag ctctttttca agagattgag cagtggcaag
     7861 gttctttctt aaggctcctt tttgttcgct aatccctgaa tgcgtttttt tggcaccgat
     7921 acccgttata cctacttgtg tgaccgctcc agtaatgctt gatgataggg ctgctaggcc
     7981 ttgccgaaca atgttctctg cagctgattt tgtagcatcg aacgcaatca atgacaattg
     8041 agagcccaat ctagtttctg cagtgcggag agcagaaagt agaacggcta cactaataat
     8101 taaagaaaca gcattagaag aaagactgga aatatccagg gtgttctctg gggtaattgt
     8161 gtgactcatt tctgaaagat ctttcatctt tttgtcagta aggcgagcaa tgatttctga
     8221 atcatgctga acctgctctg ataactttaa aagttgatca tcatttaatg tagttgttaa
     8281 tacgggattt ttaccgacat taagtggaat atgtgctgct aactgctgat aattttgtga
     8341 tttttgtgtt tcggaagaac tttgagtttg ttttgtagat atatctgtat ataaaatcgg
     8401 ggctgatttt gtgttttgaa tttccataac attctcctta tttgtatcaa gcagtagttt
     8461 gttgcaaaat tgcttttgca acatcagttc tattagcctg agagttggac attgaggcta
     8521 acagctctgc aattacttcc tgcaattggc cgaatttttc tattgcgtca ccgatgtatt
     8581 ttgacagttg ttcaacttga tatttcgata atgtcaggtc tgctagattt ttcgacgcgt
     8641 tgttttggaa aacagcagaa gcgacatttc ctcccgcttg tgtggcagag ttaacacttt
     8701 ctcctaataa aactgcttgg tttaaatggg tggaaataat ttgtttggat attacttcat
     8761 cacccgctgc accaagaaat ttatttaatc tggcaacagc attagtgatt aaatcgtcca
     8821 gttgagaaga aaaattcttg agaaactttg gtataaggtc tgtgagggtt ttacctatta
     8881 ttttgccaat attctctgca agttttgctg ctgcctgttt accaacagtg gctacgagaa
     8941 cgactgctgc aaccaagaca agagcgcctg cgattgcccc cagaatagag ccaatcattt
     9001 tggctttttt cgagtcgacg cccaagcctt cgagcatttt tgtaaatgca tctgaaagga
     9061 gtttgattaa gggttcaatg actgctttca tgatcggatt cagggcttgt tccatgaagg
     9121 aattgccggt cgctgcttgt actatagcat ccgtaaccat aagagctaaa ccaacagctg
     9181 ccagtgctag agatgctcct ccagaaaaag ctgctgcaac aacactaacg atagttaaaa
     9241 gtgccccaag tattttccca acacaaccca ttactctgtt gagttcttct gctttacgta
     9301 cttcagcagc atactcatca gattttctct ccatttcagt ttttcttgat tcttggagag
     9361 actggaatag agccagatca ttttttaaag attcttcatt attttttcca actagttgaa
     9421 taaaggttgc catcaattga gtaacactgg caagtccggt taatgatttc tgctgggttg
     9481 atagctgttc agcagatgct gtgtttgaaa atgcagaaaa agagtctatt tctttttcga
     9541 gttgcattga tttatctgta agtttgctat gaattgacag agttttctgc tcaatcaatg
     9601 tcctgtcttt aactgctgcg tcttttttga tagtgagttg tatttcttcc cgtcttaatt
     9661 ttttattttc tggtgaatct ggggcgagtt cggataatct tgtttgaatt tggttaattt
     9721 tattttctag gtcttttatt ttagaatctg cgttttttag tttattaatt tgtttttcat
     9781 agtctctggt tagtccttca gtttcagata gaagagtatt aattttatcg gagaattcta
     9841 ggtttttttg ctgtcttgcc tgttgctggg acttccaggc agtaatttta tttgttaatg
     9901 cagttaaaga tttttcacca agtatttgaa taaggtttcc aattaaaagc gttagttggg
     9961 aacttgcatt taatgacttt ggtgctttaa gttcagggat taatatattc gaagttgagt
    10021 gtgcattagt tgtattaata tttttgttag cagcaagatc agcaattgta agagaaaata
    10081 atttgttagc tgcatcattt gcagcctgga tagtattgtc tccaagctca gtggaagcca
    10141 atattttggc aagaggcaaa ccagtggttg tggtgcttac attatgcata ataattactc
    10201 cttgatatcc tgaattgcgt ctaagtatga ttgtgctttt atttttaatt tttcatcatt
    10261 gctgtgttga attacgagtt cgaagcactc tttggctttc aagggggctt tcaaccgaag
    10321 ctggcattgt ccagtatgga ataccggtgt atagtcattt tttcctaatg caaaagcgac
    10381 agcataaagg tctgctgctt gttggaactg ttcttttatc tgataaatag ctgcgagtcc
    10441 cataatgtag tctacattgt aaaagtcgta tatacataaa aatctgaaga aaacttcagc
    10501 ttcctctatt cttcctttgt tgtaaaagtc ataagcatat gaataaatgt catccatcat
    10561 atcatcagga attgcattaa tatctttcag tgtagcgcca gagttaattg catcaattac
    10621 tgcagtagag atgctttcat tttcagtgat atttaaagac ataaggtctc ctttttgtct
    10681 gcattacttt aataagtata agatttaata taaacagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tataacgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataaga
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgtgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcgt acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaaggat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgacctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caatagtttt
    11881 tattgacttt tcgataatta cttctttttt atctgttgct ccggttttta attcgtttaa
    11941 tacacgagac atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cgcatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttgggg tgtggataag gttaatttta ttgaaacttt taagatagct
    12121 acgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatctc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 attgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt cttttggcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatagctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgt ttataggtat cgacactgta acctgagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat tcccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact ggtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaacacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 actctctgcg gtattcaaca ttaatttgat gtctttacct gtaaaagaat gttgaattga
    12961 atttttgact gcttcatagg cggcaaacgc actttctgca gatttttcgc tagctaatgt
    13021 cttgaggaaa agcaataagg atttttgatt cgctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta ccaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg agaatcatac tttattaact
    13261 ctccattacg tggtgattgt ggggatgtaa aatgtatttg atatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctggttt catcatgaaa tcccacaagt taaagtgtct
    13441 gatatatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcattt caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa accaaaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagag agaaataagg gggtcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca gggaaaggcc ttttgtttgc gcgtgacaga gctgcagttt tttacgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac aattagatac
    13861 ctataaactt ggtgaagttg ataaaagaaa tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaatcgg aaataaaaca
    13981 aatatttact aattatttaa ataataagaa ttgggaaccg atcaacaaaa atattaatta
    14041 taatgggaaa aattatggtt ttcaattaac acccgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 taagcatatt gcgaatatgt ggctttcaaa agtggtggat gacgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgca gctcgtaata aagctgagga gttagcaagc gcagcattat atagcagaca
    14341 agaattatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taactggggg agaggagagt atgttaaaag atcaggtaaa
    14461 ggcattaaaa gagttaaact ctaaaagggg ggaaccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgcaagaag taaaggttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatggtga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagct atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attctaaaca agaagttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atggctctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca gggcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 gcctggaaat aaagttatga aaaaattacc gctatcttcg ttagagctgt cttatgctga
    15121 aagaataggg gaccccaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc cttcaattga
    15241 taccgatgat caagcaatca tgcttgatga tgatgtgtta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgttta gctactgatg atgaaggtgg
    15421 aagtctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggata tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt tttctgtgtt ttatcccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaacattc catcgagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tttgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tatactgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgctgat gtatggaaga gggtgggagg ctgttggtgc ctataatgca
    15901 ggaacatctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctataaacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gataatttcc cagaaaatat tattccagta acagatagta
    16201 aatcggatgg tataatttat ttggccataa gtaaagataa tatctgtcaa tttagtgatg
    16261 aaaaaggtga acaaattgat atcaattcac aatttaactc atttgaatat gacggagttt
    16321 cttttcaact taaaaatatg atggaggata aatcgagagt tcatatacta aacggaatgt
    16381 ataataaacc ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 ccctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacacagga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcatc tgtagcctta aataaaagcg gatttaccaa atgcagatat attctggtgt
    16621 ccaacaaaga aataaataga attcaacaat atatcaatca acgattccca tatatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtattttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgaga aattgaaaaa tgctctgata gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg gaggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaact aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgatcataa aaatgtatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt ttttttagat aaaaataagt gaggataaaa tgagtgttac agtaccggat
    17161 aaagattgga ctctgagttc attatctgaa acttttgatg atggaactca aacattacaa
    17221 gggcaactaa catcggcact aaatgcatta gctgaaaatc cttcgaatcc acagttgctg
    17281 gctgaatacc aaagtaaatt atctgaatat acattatata ggaacgcgca atctaatact
    17341 gtgaaagtga ttaaggatgt tgatgctgca attattcaaa acttcagata atagggagca
    17401 ttcatgaatt acatttatcc agtcaatcag gttgatatta tcaaagccag tgattttcaa
    17461 tctcaagaga tatcaagcct ggaagacgtc gtgtcggcta aatatagtga tattaagatg
    17521 gatacagata ttcaagtatc acaaataatg gagatggtaa gccatccaga atcattaaac
    17581 ccagaatctt tggccaagtt acagacgacg ctctcaaatt attcaatagg agtatcatta
    17641 gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa ctttattaaa gtcttaattt
    17701 atatgattag gtataaatgt tttattttat tcttgttgct gatgttgatt ggatgtgagc
    17761 aacgtgaaga gttaatttct aatttatctc aaagacaggc aaatgaaata atatctgtgc
    17821 tagaacgcca taatattact gctagaaaag ttgatggagg taaacagggg atctcggtac
    17881 aagtcgaaaa ggggacattt gcatcggcag ttgatttgat gcgcatgtac gatttgccaa
    17941 atccggagag agttgatatc tcacaaatgt ttcctacaga ttcattagtg tcttctccaa
    18001 gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg gctggaacag tctttagttt
    18061 ctattggtgg tgttatttcg gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata
    18121 tatcttcaaa accgatgcat atatcagtaa tcgctatata tgactcaccg aaagagtctg
    18181 aactattagt tagtaatatt aagcgatttt tgaaaaacac cttttctgat gttaagtatg
    18241 aaaatatatc tgtcatatta actccgaaag aagaatatgt ttatacaaat gtacaacctg
    18301 ttaaggaaat taaatcggaa tttttaacaa atgaagtaat atatgtattt ctcgggatgg
    18361 ctgtactagt tgtcattctt ttggtatggg cattcaaaac agggtggttc aagagaaaca
    18421 aaatatgata agaatggatg gaatttataa aaaatatctt tcaataattt ttgatccagc
    18481 gttctatata aatagaaatc ggttgaattt gccttctgaa ctgttagaaa atggcgtaat
    18541 cagaagtgag attaataatc tcataattaa caaatatgat ctaaattgcg atattgaacc
    18601 tttaagcggg gtaaccgcta tgtttgttgc caactggaat ttacttccag ctgttgctta
    18661 ttttatcggt agtcaagagt ctcggttaat taatcatagt gagatggtta ttagttatta
    18721 tgggggcaaa atcagtaagc aaggggaggc cgctattcga agtggttttt ggcatcttat
    18781 tgagtggaag gaaaatatat ctgttggtat atatgaaaga ataaacttat tatttaatcc
    18841 aattgctttg gagggaaatt atacgcctgt agaaagaaat ttgtcccgat taaatgaagg
    18901 tatgcaatat gcaaaaaggc actttaccgg tatccagaca tcatgcctat gatggtgttg
    18961 ttataaagag aattgaaaag gaattatgta aaaccataaa agatagagac actgagagta
    19021 agaagaaagc aatttgtgtt ataaaggatg ctacgaaaaa agctgaatca ctgcgtatag
    19081 atgctgtatg cgatggttat caaattggta tacagactgc ttttgagcat attattgact
    19141 atatttgtga atggaagtta aagcaaaatg aaaacagaag aaatattgag gactatataa
    19201 ctagtttgct ttcagaaaat ttacatgatg agaggatcat tagcacttta ttggagcagt
    19261 ggcttagttc gttgcgtaat acagtaacag aattaaaagt ggttttgccc aagtgtaatt
    19321 tagcactgag gaaaaagtta gaacttgatc tccataaata tcgctcagat gtcaagatta
    19381 ttttaaaata ttctgaaggg aataattata tattctgtag tggtaatcaa gtagtggagt
    19441 ttagtcctca ggatgtaata tcaggtgtta aaatagaact tgcggaaaaa ttaacaaaaa
    19501 atgataaaaa atatttcaaa gagttagcac ataagaaact gagacaaatt gcagaagact
    19561 tgttaaaaga gaatcctgtt aatgattaat caaataaatg caagcaatgc tctgcagcaa
    19621 agattaaata gtgaagaatt tgttaattta aatgagagat tatcatcttc gcaatcattt
    19681 gatgagaata taatctatga aataatgcaa tatttctcac agagtgaatt aaattccata
    19741 gataatgatg aactgcacaa taagattgaa caattattca attcacgttt tccttatctt
    19801 acagctgcac aaaaaagttc acttcttaat aaactcatag atgcaaatca atatgtcgat
    19861 ttgcatgagg gattttatgc atcattgtcc atatataaca atattgattt ctatattaag
    19921 actacaacct tcgattcatt aattagtgtc tttgaagcag ggagagaggc agatgattcg
    19981 acatggtagt aataagttga aaatatttat tttaagtata ttgctattaa cactgagtgg
    20041 gtgtgcttta aagtcatcat ctaattctga aaaagaatgg catattgttc ctgtaagtaa
    20101 ggattatttt tctattccaa atgatttatt atggtcgttt aatacaacca ataaaagtat
    20161 aaatgtttac tctaaatgta ttagtggtaa ggcggtttat agttttaatg caggtaaatt
    20221 catgggcaac tttaatgtta aggaagtaga tgggtgcttc atggatgcac aaaagatagc
    20281 tatagataaa ctattttcta tgctgaaaga cggggttgtt ttaaaaggta ataagataaa
    20341 tgataccatc cttatagaga aggatgggga agttaaatta aaattaattc gagggatata
    20401 attgtattgt gagtaaatat aaaggtctaa atacaagtaa tatgttttac atttactcta
    20461 gtggacatga accagttaac gttgagcttg taaaagataa agaacgtaac ataattgagc
    20521 tggctccagc atggaagggt ttttttttgt ccgaaatcaa aatataaaat tcagtgataa
    20581 tgtgaactat cattatcgct ttaacattaa tagttgtgca aaatttcttg ctttttggga
    20641 ctatttcagt ggtgctctag tagagcatag ccatgctgaa aaatgtattc atttctatca
    20701 tgagaatgat cttcgggata gttgtaatac cgaatcgatg cttgataagt taatgttaag
    20761 attcatcttt tcatctgacc agaatgtaag taatgcactg gctatgatac gaatgactga
    20821 gtcatatcat ttggttcttt atcttctgag aactattgag aaagaaaagg aggtaagaat
    20881 aaagagtctg acagagcatt atggtgtgtc ggaggcttat tttagatctt tatgtagaaa
    20941 agctttaggg gcaaaagtca aagaacaact gaacacttgg cggttagtaa atggtttatt
    21001 agatgtattt ttgcacaatc aaacaatcac ttctgctgcc atgaataatg ggtatgcatc
    21061 aacatcccat ttttcaaatg agataaaaac acgactaggc ttttctgcaa gagaattatc
    21121 gaatattact tttttagtga aaaaaataaa tgaaaaaatt taatattaaa tctttgactc
    21181 tcttgattgt attgttacca ttgatagtta atgctaataa tattgactct catttattag
    21241 aacaaaatga catagcgaaa tatgttgctc aaagtgatac ggtaggatct ttttttgaac
    21301 gtttttctgc gttattaaat tatcctattg ttgttagcaa acaagctgca aaaaagagaa
    21361 tatctgggga gtttgattta tctaatccag aagaaatgct tgaaaaaatg acattacttg
    21421 tgggactcat atggtataaa gatggaaatg cattatacat atatgactct ggagaattaa
    21481 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
    21541 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
    21601 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
    21661 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
    21721 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
    21781 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagccaaaa
    21841 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
    21901 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
    21961 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
    22021 tcactcagtt agatgtagcc aaacgacata tagaattatc attatggata atcgatattg
    22081 ataaatctga attaaataac cttggcgtta attggcaagg aacggcttca tttggtgatt
    22141 cgtttggtgc atcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
    22201 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
    22261 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
    22321 tgtcattagt tggtgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
    22381 atgttatacc tcgcttttca tctcagggac aaattgagat gtctttaacg atagaggatg
    22441 gaacaggaaa tagtcagtca aattacaatt ataataatga aaatacatct gtccttccag
    22501 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
    22561 taggtggtta tacgcatgaa acgaactcta acgaaatagt atctattcct tttctctcct
    22621 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag gaatatcagt aatattgtaa
    22681 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
    22741 ataagagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
    22801 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
    22861 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
    22921 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
    22981 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
    23041 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
    23101 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaactttaa
    23161 aagataacct tcctctagat caggatttta tagacagact aaagagatat tttaaagatc
    23221 caagtgatca agtcttagca ttaagggaac ttttgaatga aaaagatctt actgctgaac
    23281 aagtcgaatc attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
    23341 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
    23401 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
    23461 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
    23521 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
    23581 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gatgtaattt
    23641 ttatgaagaa actggaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
    23701 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
    23761 ctgttataga cgtatcacga gccagtcatg aggattctgt agtgtatcaa acatatctat
    23821 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
    23881 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
    23941 agatgcaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
    24001 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
    24061 attttttaat agggcttaat atcgtacttg ctattctcgt ttttatggga tcattttata
    24121 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
    24181 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
    24241 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
    24301 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
    24361 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
    24421 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
    24481 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
    24541 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
    24601 aacatggaat gtctctatct ggtgcattat ctacttatac catactaact attggtgacg
    24661 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
    24721 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
    24781 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
    24841 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
    24901 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
    24961 atattgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
    25021 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataagtgca aatgatatgg
    25081 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
    25141 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
    25201 aggtgcgtgc cgatagtttt aatatatact ttgacaaagt gtgtattaca gatgaaaatg
    25261 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
    25321 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
    25381 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
    25441 ttggtataca agaaacaaaa aatatgttag atcagtttga aaatcggtat cctgatctat
    25501 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
    25561 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
    25621 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
    25681 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
    25741 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
    25801 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
    25861 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
    25921 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
    25981 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
    26041 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
    26101 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
    26161 ttgtcaatga gcaggttatg ttatgggcta atttcgatgc accaagtgat gtaaaacttc
    26221 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
    26281 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
    26341 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
    26401 aaatcttaaa tgaggtctta taatcaatga gctatataaa attgctcact caattatctt
    26461 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
    26521 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tattgcaaga gctcaggttg
    26581 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
    26641 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
    26701 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acggataaca
    26761 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
    26821 ttgagaagcc ttttttaaca ggtgttaagg ttattgattc tttactcacg tgtggtgaag
    26881 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
    26941 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
    27001 aggttacaga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
    27061 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
    27121 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
    27181 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
    27241 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
    27301 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
    27361 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
    27421 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
    27481 ttacacaggt tgttgatgaa aaacatcgta ttatggccac tgcatttcgg gagctacttt
    27541 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
    27601 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
    27661 atcgactcgg ttttacttat gagcagacaa tggagcttct tggtgaaaca attagataag
    27721 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
    27781 acactaaaaa aaaaactctt tgtatcgaga tgtggaggca ttagataaaa ggattatata
    27841 cttcctgcaa ctggagaatg acttggaacc tgtaggcgct cagagtgtta gtcagctttt
    27901 caatacaaga cgtaagatcg ctatagtaaa aaagcatatt atccaatatc agtctgaacg
    27961 aattcttcta aagggaagaa ttgaagaaat tcaaaaagat attgatgagg caaatgcttc
    28021 taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa cggattggct tgattaaaag
    28081 aaataacttt gccaaacagt tgatacttga tgagttatca caagaggata tgaagtatgg
    28141 cattagataa tataaaccta aattttagta gtgacaaaca gatagaaaaa tgtgagaaac
    28201 tatcttcaat agataatatt gatagtctcg ttttgaagaa gaagaggaag gttgaaattc
    28261 ctgagtactc tttaattgca tctaactatt ttactattga taagcacttt gaacataagc
    28321 atgataaagg agaaatttat agtggcatta aaaatgcgtt cgaacttaga aacgaacgag
    28381 cgacatattc tgatattcca gaatcaatgg ccattaaaga aaatattttg ataccagatc
    28441 aagatatcaa agcaagggaa aaaataaata tcggcgatat gagggggatc ttttcatata
    28501 ataagagtgg aaatgcagac aagaacttcg aaagaagtca tacttcttct gtaaaccctg
    28561 ataatctgct agaatctgat aatagaaatg gtcaaattgg tttaaaaaat catagcttgt
    28621 ctattgataa gaatattgct gacatcattt ctttactaaa tggaagtgtt gctaaatcat
    28681 ttgagctgcc tgtaatgaat aaaaatactg cagacataac cccatccatg tcattgcaag
    28741 aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa aaatagtgaa atgacttacc
    28801 actttaaaca gtggggggct ggacattctg ttagtatttc agtggagtct ggttcttttg
    28861 ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga cttaattttg aaacaagatg
    28921 ctgagggtaa ttacagattt gatagcagtc aacataataa ggggaataaa aataatagta
    28981 caggatataa tgaacagagt gaagaagaat gctaagaatt aaacattttg acgctaacga
    29041 aaaactacag attttatatg caaagcaact ctgcgagcgt ttttcgattc agacattcaa
    29101 aaataaattt acaggcagtg aaagtttagt cactcttact tccgtgtgtg gggattgggt
    29161 aattcgtatt gatacattat cttttttgaa aaaaaaatac gaggtatttt caggattttc
    29221 tacacaagaa tctttactgc atttatcaaa atgtgtcttt atagagtcgt catctgtatt
    29281 ttcgattcca gaactgtctg ataagattac tttccggatc acgaatgaaa tccagtatgc
    29341 aactactggg agtcatttat gctgtttttc ctcttcttta ggtattattt attttgacaa
    29401 gatgccggta ttacgtaatc aagtttctct tgacttattg catcatcttt tagagttttg
    29461 cttaggttca tctaatgtaa ggctggctac tttaaaaaga attcgtactg gtgatataat
    29521 catagttcag aaactttata atttattatt gtgtaatcaa gttattattg gggattatat
    29581 tgtgaatgat aataatgagg caaaaattaa tctgtcagaa agtaatggtg agtcagatca
    29641 cacagaagtt tctttggcat tattcaatta tgatgatatc aatgtaaaag tggactttat
    29701 ccttttagaa aaaaagatga caatcaatga actaaaaatg tatgtagaaa acgaattatt
    29761 caagtttccc gatgacatag ttaaacatgt aaatattaaa gtaaatggtt ctttggttgg
    29821 gcatggggaa cttgtttcta ttgaggatgg ttatggtatc gagattagtt cttggatggt
    29881 aaaggagtaa tctcatgctg agtgatatgt ccctcatcgc tacactttct ttttttacgc
    29941 tattaccatt tttagttgct gctggtactt gttatataaa gttttctatt gtttttgtaa
    30001 tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa tatgactctc aatggtattg
    30061 cattgattat ggctttgttc gtgatgaaac ccattattga agctggttat gaaaattatc
    30121 taaatggccc gcaaaaattt gatacaatat cagatattgt ccgtttttct gattctggct
    30181 tgattgaata caagcaatac ttgaaaaagc atacagatct tgagttggca cgttttttcc
    30241 aaaggtctga agaagaaaat gctgatttaa agagtgcaga gaataatgat tattcattgt
    30301 tttccctctt gcctgcatat gcgctaagtg aaattaaaga tgcgttcaaa ataggttttt
    30361 atttatattt gccatttatt gttgttgatc ttgttatttc aagcatttta cttgcacttg
    30421 gtatgatgat gatgagtcca atcacaatat cagtaccaat aaaacttgta ttatttgttg
    30481 cgcttgatgg gtgggggatc ctgtctaagg ccttgattga acaatatatc aatgttcctg
    30541 cttagatgat ggaggtaatg tgtctgatat agtttatatg ggtaataagg ctctttattt
    30601 aatccttatc ttttccttat ggccagtagg tatagctacg gttattggat taactattgg
    30661 tttattacag acagtgactc aacttcaaga gcagacactt ccttttggta taaagcttat
    30721 aggtgtctca atatctttgc tacttctttc tggatggtat ggtgaggttt tattgtcttt
    30781 ttgtcatgaa ataatgtttt taattaagag tggggtttga tggacatttc aagctggttc
    30841 gaaagtattc atgtgttttt aatactcctg aacggcgttt tttttagatt ggctccatta
    30901 tttttctttc ttccattttt aaataacggt ataatttctc catctattag aatacctgtg
    30961 atttttcttg ttgcatcagg attaattact tctggtaagg tagacatagg ttcttctgtt
    31021 tttgaacatg tttatttcct tatgttcaag gaaataattg ttggcctcct tctctctttt
    31081 tgcttgtctc ttcccttttg gatatttcat gctgttggta gtattattga caaccagcgt
    31141 ggggcaacgc ttagtagttc aattgatcct gccaatggtg ttgatacgtc tgaattggca
    31201 aaatttttca atcttttttc tgcagttgta tttctataca gtggtggaat ggtctttatt
    31261 ttagaatcca tacaattgtc ttataatata tgcccgttat tttctcaatg ttctttccgt
    31321 gtctcaaata tcttaacatt tctgacttta ttggcaagtc aggctgttat tttagccagt
    31381 cctgttatga tagtattgtt actatcagaa gtattacttg gtgtattatc gagatttgct
    31441 ccgcagatga atgctttttc cgtatcatta actattaaaa gtttacttgc aatatttatt
    31501 attttcatct gttcttctac tatttacttt tctaaagttc aatttttcct cggtgaacat
    31561 aaatttttca caaatctatt tgttagataa aatattatgg caaataaaac agaaaagccg
    31621 acacctaaaa aactaaagga tgccgcaaaa aaaggacagt catttaaatt taaggattta
    31681 acgacagttg ttattattct ggtagggaca tttactataa tatcattctt ttccttaagt
    31741 gatgtaatgc tcttatacag atatgtaata attaatgact tcgaaattaa tgagggtaaa
    31801 tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact ttttttctgt
    31861 gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc gactaaagct
    31921 atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat atttagtata
    31981 aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc attaacaacc
    32041 tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc tagtgttgat
    32101 ggcttatatc ttatttgggg ggggctattt aaggatataa tattattttt cttagcattt
    32161 tctatttttg ttattattct tgactttgtg attgagttca ttttatacat gaaagatatg
    32221 atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca ctttgagaca
    32281 aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa atctgatata
    32341 cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat ttattttaat
    32401 ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg tgccttggct
    32461 gtcagaaaat atgcaaatga agttggtata ccaactgtgc gtgatgtgaa attagctaga
    32521 aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt ggatgaagtc
    32581 ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt aaaggagatg
    32641 tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga atttgaaata
    32701 gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc tttccttagc
    32761 aacaaggata taataaatgt tttactcaga aagatttcat ctgaatgtga cctggttaga
    32821 aaagacattt atcgttgtgc tcttgagttg gtcgttgaaa aaacacctga tgatttataa
    32881 tcattcataa taactctttt gttgatataa atatttttgt tgaagatatt tatgtagtca
    32941 caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgaactaaa taatatatga
    33001 taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat aagagagact
    33061 attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca aagtctgtaa
    33121 atgatcatga gatactaacc gagagtaatc gtttattatc tcattgcatt tcagatagta
    33181 acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt aaaaggagaa
    33241 tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt aaactatatt
    33301 gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt ttttgatatg
    33361 tttactccaa caacg
//
LOCUS       DADLTD010000021        32956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLTD010000021 DADLTD010000000
VERSION     DADLTD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.021"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..257
                     /locus_tag="JN701_000803"
     CDS             <1..257
                     /locus_tag="JN701_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY7363333.1"
                     /translation="LDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSVV
                     MTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            267..1641
                     /gene="gspE"
                     /locus_tag="JN701_000804"
                     /pseudo
     CDS             267..1641
                     /gene="gspE"
                     /locus_tag="JN701_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1631..2833
                     /gene="hofC"
                     /locus_tag="JN701_000805"
     CDS             1631..2833
                     /gene="hofC"
                     /locus_tag="JN701_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY7363334.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="JN701_000806"
     CDS             complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="JN701_000806"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY7363335.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            4136..4756
                     /gene="coaE"
                     /locus_tag="JN701_000807"
     CDS             4136..4756
                     /gene="coaE"
                     /locus_tag="JN701_000807"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY7363336.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4756..5499
                     /gene="zapD"
                     /locus_tag="JN701_000808"
     CDS             4756..5499
                     /gene="zapD"
                     /locus_tag="JN701_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY7363337.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5509..5706
                     /gene="yacG"
                     /locus_tag="JN701_000809"
     CDS             5509..5706
                     /gene="yacG"
                     /locus_tag="JN701_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY7363338.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="JN701_000810"
     CDS             complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="JN701_000810"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY7363339.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6262..8967)
                     /gene="secA"
                     /locus_tag="JN701_000811"
     CDS             complement(6262..8967)
                     /gene="secA"
                     /locus_tag="JN701_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY7363340.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9029..9541)
                     /gene="secM"
                     /locus_tag="JN701_000812"
     CDS             complement(9029..9541)
                     /gene="secM"
                     /locus_tag="JN701_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY7363341.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="JN701_000813"
     CDS             complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="JN701_000813"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY7363342.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="JN701_000814"
     CDS             complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="JN701_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY7363343.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="JN701_000815"
     CDS             complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="JN701_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY7363344.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="JN701_000816"
     CDS             complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="JN701_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY7363345.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14093..15013)
                     /locus_tag="JN701_000817"
     CDS             complement(14093..15013)
                     /locus_tag="JN701_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY7363346.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(15006..16481)
                     /gene="murC"
                     /locus_tag="JN701_000818"
     CDS             complement(15006..16481)
                     /gene="murC"
                     /locus_tag="JN701_000818"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY7363347.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16535..17602)
                     /gene="murG"
                     /locus_tag="JN701_000819"
     CDS             complement(16535..17602)
                     /gene="murG"
                     /locus_tag="JN701_000819"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY7363348.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="JN701_000820"
     CDS             complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="JN701_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY7363349.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18843..20159)
                     /gene="murD"
                     /locus_tag="JN701_000821"
     CDS             complement(18843..20159)
                     /gene="murD"
                     /locus_tag="JN701_000821"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY7363350.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="JN701_000822"
     CDS             complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="JN701_000822"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY7363351.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21238..22596)
                     /gene="murF"
                     /locus_tag="JN701_000823"
     CDS             complement(21238..22596)
                     /gene="murF"
                     /locus_tag="JN701_000823"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY7363352.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22593..24080)
                     /gene="murE"
                     /locus_tag="JN701_000824"
     CDS             complement(22593..24080)
                     /gene="murE"
                     /locus_tag="JN701_000824"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY7363353.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24067..25833)
                     /locus_tag="JN701_000825"
     CDS             complement(24067..25833)
                     /locus_tag="JN701_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY7363354.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="JN701_000826"
     CDS             complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="JN701_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY7363355.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="JN701_000827"
     CDS             complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="JN701_000827"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY7363356.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="JN701_000828"
     CDS             complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="JN701_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY7363357.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28214..29218)
                     /gene="cra"
                     /locus_tag="JN701_000829"
     CDS             complement(28214..29218)
                     /gene="cra"
                     /locus_tag="JN701_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY7363358.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29300..29386)
                     /locus_tag="JN701_000830"
     CDS             complement(29300..29386)
                     /locus_tag="JN701_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY7363359.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="JN701_000831"
     CDS             complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="JN701_000831"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7363360.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="JN701_000832"
     CDS             complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="JN701_000832"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY7363361.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="JN701_000833"
     CDS             complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="JN701_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY7363362.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7909 a         9256 c         8020 g         7771 t
ORIGIN      
        1 gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccgctatg
       61 tttcagccat gagtgtggca aaaggcgtgg tgtcgctgac cgggcaagaa agtctcaatg
      121 gactaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc ggctgggcgc
      181 gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat gtcttccgct
      241 ttgatgacgc caactaagga gcggcaatga atattccaca gctcaccgcc ctgtgtctgc
      301 gttatcaggg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg gtagtcgatg
      361 caccttcgca tgagttgctg gacgcattgc atttcgctac caccaaacgt attgagatca
      421 cctgctggac gcgccaacaa atggaaggcc acgccagtcg cacacaacag acattgcccg
      481 tagctgttca ggagaagcat cagtcgaaag cagagttgct gactcgaacg ttacaatctg
      541 cgctggaaca acgtgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc
      601 gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg
      661 cattaaccgc cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc
      721 aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt attgcgacct
      781 taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg aaccaggcac
      841 tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct catgccttgc
      901 aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa acggtcacgc
      961 tttatagtgc cctgcaaaag ctgaataccg ctgacattaa tatttgtagc gtcgaagatc
     1021 cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt gccgggctca
     1081 cctttcaggg cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc atgatccgat
     1141 ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt gttgtctacc
     1201 ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg agtcgcccgc
     1261 tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg taaattttgc
     1321 ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt atggccgtcg
     1381 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
     1441 accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat
     1501 accgacgttg aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac gctttttgaa
     1561 aacggctgcc tagccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
     1621 gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
     1681 tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgctcatgg cactacagca
     1741 acagatggtt accccactta gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
     1801 agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct
     1861 ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
     1921 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
     1981 gtcagaggta tttccgccac tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
     2041 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga
     2101 caaagtgaaa tcagcgttac gttatcccat catcatttta gcgatggcaa tcatggtggt
     2161 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
     2221 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatggag
     2281 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
     2341 accgacctgg cttatagtgc ggcagaaatt gctgttacgc atcccgatta tgggttcact
     2401 gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
     2461 cattactttt ttgcagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
     2521 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
     2581 aaaaaatacc ggggagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc
     2641 cggctcgctg gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
     2701 gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat
     2761 tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg atgcgatgag
     2821 tgggatggga taacgctggc gtgttgctcc acaccagcgt tggaagatta caggttgttg
     2881 aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt cagttctttc
     2941 aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg
     3001 gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcgatat
     3061 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
     3121 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
     3181 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg cttttgcgac atcgcccggc
     3241 gtggtgcagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat
     3301 tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca
     3361 gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac
     3421 atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct
     3481 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacaaag
     3541 tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgta
     3601 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
     3661 cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga agccagcgca
     3721 gaggccatag aaaatgtacc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
     3781 cagctctgat ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
     3841 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
     3901 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
     3961 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
     4021 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc
     4081 gtgcactcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatgag
     4141 gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt
     4201 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
     4261 aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga ttgctgctga
     4321 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
     4381 ctggcttaac gccctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca
     4441 agctacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
     4501 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
     4561 gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc aggcaacgcg
     4621 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
     4681 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc
     4741 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
     4801 cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt
     4861 gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
     4921 cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg taaactccag
     4981 acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
     5041 aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
     5101 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
     5161 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
     5221 acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt
     5281 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
     5341 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
     5401 tccggacata agagccgttt tgccattcgt tttatgccgc tggacagtga aaacggacag
     5461 gtaccggaac gtctagattt cgaactggcc tgttgctaag gagtgaaaat gtcagaaact
     5521 attacggtga attgcccaac ctgcgggaaa acggtggtat ggggtgaaat cagcccgttt
     5581 cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa
     5641 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag
     5701 cagtgacatt ggtctgatgc cgcacatagg cctgataaga cgcggacagc gtcgcatcag
     5761 gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gactacagac
     5821 gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca ttaagaccga
     5881 ccagcgacat ccactcaccg ggttgccctt ctttacccca cggcacccct tcccaacttt
     5941 cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatatgcc agtttttcaa
     6001 atagcgaaaa atgttgtggg gtaatcccga cctcttcctg aagttcacgc accaccgcct
     6061 gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta ttcgccatgt
     6121 gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga ataataccta
     6181 ccgcaatttg cagctttttc attgttttaa acctataaaa aaggcgcaga atcctgcgcc
     6241 ttttgcttca acagttagct tttattgcag gcggccatga cactgcttgt attttttacc
     6301 agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt gcgccgccag
     6361 tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt gcgctaaacg
     6421 ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca tacgtacctg
     6481 aactttgctc agcgtactga taacttcata tttcaacgac tccagcatcg ctgcaaacat
     6541 ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc cacgcaggtg
     6601 gataccctga cgcagatagt ccatcgctgc caggtgctct ttccacaggg agtcaagcgt
     6661 ttgcagcatg acgcctttct cgaagtgacg catcatctca gcaccaacca cttcttcttt
     6721 acgctgatac acttcgatgg actgcgccag aatgcgctca cgcagcgtct cttcatgcag
     6781 ttctggttct ttatccagcc actcggcaat tggtaaatcg aggtcgaaat cgttcttcag
     6841 acgttcctgc agccccggaa tatcccacat ttcttccagc gactgtggcg gaatgtaggc
     6901 atcaatggtg gctttgaaca catcttcacg aatgctgttg atggtttcgc tcacatcgct
     6961 gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt tagccacgtc
     7021 atcatattcc agcagttgct tacgaatgtc gaagttacga ctttcaactt tacgctgggc
     7081 gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct tcatacccag
     7141 tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg catcttccat
     7201 cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac cgcgcaactg
     7261 gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc
     7321 cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt gctctgcggt
     7381 cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa tatctgtacc
     7441 acgacccgcc atattggtcg cgatagtcac cgcagccgga taacctgcct gagcaacaat
     7501 cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac cggctttggt
     7561 cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca gcaccggctg
     7621 gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt
     7681 catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg gaacaacgac
     7741 ggtatccagc ttgtagatgg agctaaattc gaaagcttcg gtatcagcag taccggtcat
     7801 ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag ccagcgtttg
     7861 gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca gaccatcgga
     7921 ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa cttcaccatc
     7981 tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg ccgccgttac
     8041 gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat ccatgatgcc
     8101 ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt tcacctggcg
     8161 agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt cttctttttc
     8221 ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg agctgtcttc
     8281 tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg agtccacttc
     8341 gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga acgccatatt
     8401 gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta
     8461 agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccgacag tcaggccaag
     8521 gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt cgttgacggt
     8581 aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag
     8641 ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc
     8701 gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct cacgtaccac
     8761 ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac gtgcacgaaa
     8821 ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg gttccatggc
     8881 attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa
     8941 aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg ttgcggagtt
     9001 aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca gcacggatgc
     9061 cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat
     9121 aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg
     9181 tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca acgggcagtg
     9241 tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt gcgaatggca tattgatgcc
     9301 agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc aaggccaatt
     9361 gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt gcgggcacgt
     9421 ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcgacc atccctaata
     9481 agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt attccactca
     9541 cgttattgcc atcccgatcg aattcaaacg tatttagcgc acaaatctta tcattaaagc
     9601 ctgaagtcca cagcatgaat gaagataagt tgtgcgtaaa agtgcttaag aaaacagcgt
     9661 tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa acgactggtt cacctggccg
     9721 gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc gttatgccag
     9781 tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga aggtcacata
     9841 ttcccaagct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca gtgcatgacc
     9901 ggatttataa gcgataaatg caccaataat attgtgacca cacatgaaca agtcaccgat
     9961 cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag
    10021 tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc acaaaccacg
    10081 ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc tgatctggcg
    10141 cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat caatagccgg
    10201 atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa attcagccca
    10261 cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt tggcgcagtt
    10321 caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc tgccgtccat
    10381 gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca ggcccgcgag
    10441 agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga ccagacacgt
    10501 acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg gtggattcaa
    10561 gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg tcagagtgac
    10621 tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg
    10681 tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact atacattcgg
    10741 cgggccagtt tagcacaaag agcctcgaaa ccaaaattcc agtcaattct taatcagctt
    10801 gcttacgcag gaatgctggg atatccagat aatccggctc tttcgcagtt tgcggcgcat
    10861 tgtcattcac gactttagca accggcttct gctcctgggt cagcggagcc atcccatgct
    10921 gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag
    10981 gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg tcattcatat
    11041 ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg
    11101 tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg atgttaacca
    11161 gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag atagccattt
    11221 cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc attgcgtagc
    11281 ccatctcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa cccggacgag
    11341 taatcagttc agcgatacct tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg
    11401 catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc gggatagtga
    11461 tcagagagtc cacatgcttg gacagttcag tgatcccctg ctccgcgaat gccatacgct
    11521 tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata cccaaatctt
    11581 ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc atacccgcag
    11641 caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag
    11701 ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg ataccgctac
    11761 cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg
    11821 cgaagaattc aacaccttca atgcgctcgc gcaccatgtg ttcaacagca ttaccgccgc
    11881 cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt ggttcaaaca
    11941 tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg cctcataaaa
    12001 attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca actgatgctg
    12061 taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca tagtgaagca
    12121 atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg gtaatgttca
    12181 gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct gcaagacctt
    12241 cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt ttaacccctt
    12301 gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg agcagctcgg
    12361 tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgt ggcggacgac
    12421 cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc agcgcacaac
    12481 cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat
    12541 cactggtcac gacattgcca gcataaggaa ttaccttagt gtggcgcaat gccccaccgg
    12601 tataaacggc gatatccatt gtaccacctc cgatatcgac gacgcagaca cccagttcac
    12661 gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc agttggtcaa
    12721 ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg ttgtgacatg
    12781 tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag tcctaccgga ttcttgatcc
    12841 cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga tgttcatcgc
    12901 gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc gtcacttctt
    12961 cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta ccagaaagcg
    13021 ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca atggcgcgtt
    13081 gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg
    13141 ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc cctactaaag
    13201 cggcaacctt cgcggtacca atctccagtc ctactaccag ttttctgtcc gtcgccttga
    13261 tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg gcggcaaggg
    13321 cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa tccgtttgcc
    13381 atcggtttgc gccagctgct gtaaaaccgg ataaagttct acaaagcgag ccaaacgttt
    13441 catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca actgccagga
    13501 acgccgcgcg gtcatcgccg tttccttcag agtaaatctg tcctttgcca gcatctgccc
    13561 catttcgcga tagccctgca acacttcatt ggcgctgcct tccgggccat acagcattgg
    13621 aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac
    13681 catatgttga tcattccacc gcgcaatcgg cacatattca accagatgaa tcttcaattc
    13741 atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt gttctatttg
    13801 cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca atgccaggat
    13861 cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca ccagctttga
    13921 gagcggcagg cgttgcgcat cttccatcca gcccaacacg acccagccgc tcaccaacac
    13981 tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat tattgcggcg
    14041 agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca tattagtccg
    14101 ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga cgtgccgcca
    14161 tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc agataaaact
    14221 gtccatcgct gtccagcata acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc
    14281 atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcatccaga cctgcggggc
    14341 agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag gttccggacg
    14401 gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg aactccggcc
    14461 cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat cttaatgcat
    14521 cttgtagagc attttctgct actacttttg acattcccac actggaacct tcgcggctcg
    14581 gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta tcgctcaggc
    14641 ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa ccggcacctt
    14701 gccacagcag tttgctgcgt agtttatcca ttgaaagcgc agatgccatc actccgctgc
    14761 cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct tcaccgccgc
    14821 gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc gtcacgtcga
    14881 cttctttcgg gtcgacagga tacgcatcaa taccaccttc acgcagcccg gctaacaccg
    14941 ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac aggaccgcga
    15001 ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat ttcagctaaa
    15061 gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc gttaccggtt
    15121 aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat gggatcaatt
    15181 ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg aattggcgct
    15241 tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag cacattggcg
    15301 aaatcatcat acaagtcgcg cgtacgggta aaacggtgcg gctgaaacag cattaccagg
    15361 tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg
    15421 tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg ctccagcggg
    15481 aattcaccga ggaaatcaaa acggcgacca gtccccagga agctctccag cgcacgcaaa
    15541 atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc
    15601 gcgttatgac ggcctggcgc attcagggtg acgcgcatcg gctctttgtc ctggcgcagc
    15661 agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac gtcggcatct
    15721 tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg gtaacaattc gcggatcacc
    15781 ggatcatcaa cacacatcac cgcacgaccg taaaacggca ggttgtgcag aaaattaata
    15841 aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata
    15901 ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact ctcatctgct
    15961 tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc
    16021 ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg
    16081 gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa acgcattaac
    16141 tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc tgcgacaatt
    16201 tccgggttat cggcagaaat ggcgctggaa acaacgacca cgctggcatc acgtacgttt
    16261 tccgggcgat ggttgaaata aatcgtcgct cccagattca ttaactgctg cgtgaccgga
    16321 tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac ttcggcaata
    16381 ccgcccatac cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg acgcatttcg
    16441 ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc cattaacttc
    16501 ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc cggctcactt
    16561 catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg
    16621 ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc acgctaagct
    16681 gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca ttccagtatt
    16741 gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc gcggcgattt
    16801 cactcaccgt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca tacgccgccg
    16861 ccatatcatc aataaattcc gtcactttat gctgcggttg ccccgcttcg gcatacgcct
    16921 gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca gatagtgact gaatcaccca
    16981 gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg gaaccaccca
    17041 ctaccagcac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg
    17101 ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa gcgcctggaa
    17161 acgcctgcat cactttggtg gcaatcttcg ccagccattt attggttaag cccgcaatac
    17221 cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga ccacctggac
    17281 ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc ataatcgccc
    17341 gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct tttatacctt
    17401 ttccacgcag accagagata cgaatgaaat caatttcgat gccatgtttt ggcactaagt
    17461 ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga gccattagat
    17521 ggtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc atcaccatta
    17581 atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg
    17641 cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca gtaagctcga
    17701 accaccgtaa ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc
    17761 gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg cgagaaaacc
    17821 ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc gaaaagcgac
    17881 gaagaatacc attaaaagtg ccagcaccac accgacatac cccagttctt cgccgataat
    17941 ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta ccgagttacc
    18001 taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg ttaactgata
    18061 gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc ggatacggta
    18121 cggttcggcg agtatcagca acacaaccgc tgaaatgccc ataccgataa tggcaatgaa
    18181 ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa acaccaccac
    18241 caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa tcacgcccat
    18301 cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca ccagatagtt
    18361 ggcgatataa caaaacagcg acagttttgt cagctccgca ggctggatac gcagcaaacc
    18421 gagatcgatc caacgcgatg cccctttaac cgagctcccc actaccagca cgatcatcag
    18481 caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact ccatcggcag
    18541 acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat cacgcttcgc
    18601 gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg aggtcaccat
    18661 gataaagcca atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat cgtacatgat
    18721 caggctgtcg gtatcttttt cccgcgagcc catcacccag ccttttagcg ccgtggagat
    18781 ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga gagataaacg
    18841 catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc aaagttcttg
    18901 aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg ctgaacacgc
    18961 ggagccagca agcgcatcgc ctgttccata gtttcggttt gttctgccac ttccgggcgt
    19021 agcgccgcca gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt gtcgccattc
    19081 agatagcgcg ccagtggact gaagtccgcc gatttaccat cgccgcccag caacaaatgc
    19141 agcgtgccgt ctacctgcaa gccattcaac gccgcttccg tactgccgac gttagtggct
    19201 ttcgaatcgt taatccagcg tacgccattg tgctccagca ccacttcaaa gcgatgcggc
    19261 agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc agctgcgtct
    19321 gccagtgcca gtgctgccag cgcattggtg tagttatgct gcccggaaag tttcatctct
    19381 ttcacgttca gcactttctc gcctttcact cgcagccagg tttctccctg ctgatgattc
    19441 aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatcagc accgcgaatc
    19501 ggcattgtta aggcatcatc agcattgacg acacaaactt tcgcattttc gtaaatacgc
    19561 aattttgccg cacggtattg ctgcaaacca aacggatagc ggtccatatg atcttcagtc
    19621 acgttcagaa tggtcgccgc caccgcctgt aagctggaag tggtttccag ctggaagctc
    19681 gacagttcca gcacgtacag ttcgcactca gcatcaagca gcatcaacgc tggcaggcca
    19741 atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc cactagcgtg
    19801 gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg tgcttcgcgg
    19861 cagaatagct cgatatcgcc aacgatttcg attccggcat cagcggcagc gcttaaggat
    19921 ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat cagccattca
    19981 tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag gccaggcggt
    20041 gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa gtccacgcag
    20101 gaaagcccgg tgaggcccag gccgataatg acgacatttt taccctgata atcagccatg
    20161 attaacgtac cttcagcgtt gccagaccaa tcagaaccag catcagcgaa ataatccaga
    20221 aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga tgaatcggtg
    20281 ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg atgacagaaa
    20341 gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga cgtagcagta
    20401 cggcgataat gcctaacgca ccacctaacg ccagcgaacc tacatcgccc ataaagacct
    20461 gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc gcggtacaga
    20521 caataaccag ttccccggcg tgtcgcagat acggtatgtg taaatagctg gcaaagttca
    20581 tgttgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg gtcggcataa
    20641 ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc acaatgacga
    20701 agtaagccag cagaatgtag aacagcccca actgcggcat cacatcttta aagaatggga
    20761 ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag gcgacaccca
    20821 gcgcaatgac cgacatccag aaatacttcc aacgagcgat caaccctttg gtgtctttac
    20881 gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc agcaccacca
    20941 acacgcacca gacgtacgga ttggacgggt aagcccacag cagtacggag atcacaatcg
    21001 ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag tgtgattcag
    21061 gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg caaatgagca atcatacgcg
    21121 ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg cgaaacgtca
    21181 gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc agccaaacta
    21241 acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcggcac tacgtgaacc
    21301 cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac gcgtaattaa
    21361 cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag catggctttg
    21421 tttacccacg cttaacacgc ggtcaatacc agcagctttt gccgcctcgc ccacctgtac
    21481 atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca gcacgcggta
    21541 gcccggcatt tcagccagta cctgaacagc ggcggtcatt gaaccgacat tggcgttgta
    21601 ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac ggcctggaac
    21661 agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca cggacatgga
    21721 gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg gcagcagaac
    21781 atcgacgcta ccggttgggg tttgtagggt aaattccgta ccgtgcgagg tcacatggat
    21841 attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca ctttgcgtga
    21901 gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa tggcgatacc
    21961 gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg caagcgagcc
    22021 aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gcagcttccg ggcgagtcag
    22081 actcacagtc caggctattt cgccctgatg gttcgcgcca agttcaataa ctgcgtaatc
    22141 gtattccggc gttaagcgca acaacgtcat cggtacaccg atgtcgttgt tgagattgcc
    22201 tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct ctttaacgga
    22261 ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct ggcgaaccca
    22321 tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg gcaggtcgat
    22381 atccagcgga cggctgacca gtagtgcgcc cgcgccgcca gctttcgcct ggtcggcaaa
    22441 atcgtgggca tcaaaacgtt cgcctttcag ggcaacaaac aggcagcccg gcgtcagttt
    22501 tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt caccgttgag
    22561 aatgtcggta agttggctaa gggttacgct aatcatgcaa tcacccccag cagacgcgcc
    22621 accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta atcttcatgg
    22681 cctttgcccg cgaccagcac cacatcgttc tctttagcct gcataacggc gcaagtcacc
    22741 gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc cgccagaata
    22801 tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac caccgccacg
    22861 tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc gcgatcgcca
    22921 ccacagccaa agacacacca cagcttgccc gcacagtgca gacgcgccgc ctgtaaggct
    22981 ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt gcctggcaca
    23041 gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt caacagatca
    23101 gccaatggat agccgagtgc caacagtgtc gccagcgcga gcagcaggtt gctgacgtta
    23161 aaagcgccta tcagatggct ttcaatttcg ccatcgcccc aacttgagct aaagcgaatc
    23221 gtcacaccgc tgtcgtgata gttcacgtcg atcactttta accagcgtcc gtgacagttc
    23281 ggattaatat gatcttccat tgataccgca accgcgtccg gcagctttgc cagccaacgg
    23341 cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc agaataaagc
    23401 agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg atcgcggctt
    23461 aagttggtaa agaccgacgc cgcaaatttc aatgccgcca cacggtgctg taccagcccg
    23521 tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct ccaccagccc cgccagctca
    23581 tgctgaacat cgactgccga accggtggta ttttctgtcg ggatcacttt ccccagcaga
    23641 ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct ccactgcgcc
    23701 agcagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag acgtaaattg
    23761 tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt gagctggctg
    23821 agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc ctcatctttc
    23881 gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata tcgacgcccg
    23941 tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac acggctgtcg
    24001 agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc aagaaggtcg
    24061 cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt catttttatc
    24121 gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc ccatgatggc
    24181 accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg gatcgttgat
    24241 aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg tataagcaat
    24301 atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg ttttaatggc
    24361 gatacgatag cctttaatcg ccgccttcac gccgccgccg cccggtagcg ccacgctttc
    24421 catcatatgc accacggtgc ggacaatgga ttccgggaag acacgttcac cgggaaccgg
    24481 ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga tagttgcgta
    24541 gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga aggtggccct
    24601 ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc cgaccaaccc
    24661 caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg ctgaggacgg
    24721 catcgctaac gccagcttgg aaacaccgac gttactcgac ttctgtaata ccccggtcag
    24781 ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc gataaggaat
    24841 ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca tcaccaccat
    24901 cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac gcatcgcctc
    24961 tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca tcgccagcac
    25021 ttcacctgtg ttgacatcca ccagcacggc gctaccagat tcagccttgt taaaggccac
    25081 cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac tcagcgccag
    25141 gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac catagcggtc
    25201 tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac tcttctcaac
    25261 gccctcaatc ccttgactat cgacgttagt aaagccaatg aggtgagcag tcacttcgcc
    25321 ggatggataa taacggcgag attcttcacg cagatgaata cccggcagtt tcagtttttt
    25381 gatgtagtcc gccatgtcag ggtttacctg acgcgccaga taaataaagc gccctttcgg
    25441 gttggcgtta atgcgggcag aaagctgatc cagcggaata ttgagcgcgt tagccagcgc
    25501 cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg ggtcagccca
    25561 aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag taatcatgcc
    25621 gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt tcaccagcat
    25681 atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca gcgccaggag
    25741 aatacagccg cataacaacg caaaacgcca actaataaag ttggcatgtt cttcctgacg
    25801 ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt atttttgcac
    25861 tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg tggcgatcct
    25921 ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc gccattcaat
    25981 gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta aacgggtatg
    26041 gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca ggcagagtgg
    26101 cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat ggcgctcgtg
    26161 gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga tcatgcgttc
    26221 gtcctctctg cgatacgcag aactgaacta cgggcacgag gattctcagc cacctcttct
    26281 tcgcccggca ttaacttgcc taatgctcgc agctgacggc cacccagttt tttgagctgc
    26341 tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc acgcataaaa
    26401 cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg cccacccggg
    26461 gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc actgtttacc
    26521 caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc tttcaccggc
    26581 gttgcagcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg ttcgcggtta
    26641 cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata ggttttcaat
    26701 acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga ctgcccacgg
    26761 gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc acgttcagca
    26821 tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat cttgccgata
    26881 agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat gatggagaag
    26941 cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc gatcgccagc
    27001 aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc gcgaccaaaa
    27061 gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc ttcatccagc
    27121 agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct gcagtcgctc
    27181 cgataagtct ccagtagcca actgctctgc gtcgatatct tccttgacct gttgatgcca
    27241 ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca cttcttttgt
    27301 cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc catccatctg
    27361 acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaactg ggttcatgct
    27421 cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt aaagcagcag
    27481 gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct caagcagctg
    27541 ttcccgataa cgggtaggca ctgataagcg ccctttgctg tcgagattga ctaacgttgc
    27601 tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt tcccactgaa
    27661 aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata ccgaggtaaa
    27721 aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg cgcgaagcaa
    27781 attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcactgt tttaaaatga
    27841 ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg cattttagga
    27901 catatcttcc ccggttaaca gtccttgtta cgtctgtgtg gcgcttgatg ccagcgggtt
    27961 tgcgcaaaag caaagaaaag tgtttgttaa atcggcaatt catcgccgca tgtaacaaaa
    28021 taatgcaaaa acgcgctgtc gatatcactt aattattgtg aatttcagct ggtgagttaa
    28081 ttaacgggcg ggtttaaaac catttatcaa taatagtcag gaatagtctt atttacttta
    28141 agcatattga tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat
    28201 ttttgttcgc ggcttagcta cggctgagca caccgcggcg atagagatta cgtttaatgc
    28261 gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca
    28321 cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac tgtaagaagt
    28381 cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttaccatcgc
    28441 gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg aacagcgcct
    28501 gcggcatcag atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat
    28561 agctgttggc atacaggaaa tgcacttcgc gcggatcatc tttccaggca gtacggaaac
    28621 cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct
    28681 cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa
    28741 ccacgctggt gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggg
    28801 cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg
    28861 catcaacctg acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt
    28921 ctgagcaggc aatcagcagt tgataacccc gttgccgcgc ctggcgctca agatagttag
    28981 cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc
    29041 gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag
    29101 ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa
    29161 catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa
    29221 ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt
    29281 aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt
    29341 tcgcaaaaaa agcccagcgt gacatgttgg gctgcaaatt gcacactgag atcattctca
    29401 acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat
    29461 tttcgccacc tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa
    29521 ttgaacggta taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt
    29581 acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg
    29641 ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa
    29701 ttgcttttcg atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga
    29761 taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga
    29821 aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat
    29881 ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg
    29941 cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg
    30001 cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga
    30061 gagatctgga tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat
    30121 tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc
    30181 cccagatagc ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta
    30241 gacagttcct gaatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct
    30301 tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgagg
    30361 ccaccggaat tgatccagcg acgcggtttg tcgaatggat aataaagtgc agcaaacatc
    30421 tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg ccaaagagtc
    30481 tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgacga
    30541 gcgcgccact gttcaatttg ctgccaccag tcgcggatct catccagtgg ttgatgggcg
    30601 gattcttgcg acaagagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc
    30661 gggatatccg cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca
    30721 gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg
    30781 acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt
    30841 cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag
    30901 gaaacaacgg gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc
    30961 atgattgccc caccgcctac gtagacaacc ggtttttttg ccactaccag cgtttgcaga
    31021 gcacgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc
    31081 gactccggcc agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca
    31141 acgactactg gtccaggacg accacttgcc gccagccaga aagccttttt cagcacctgc
    31201 ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccactggtcg cgaaatcccc
    31261 accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa
    31321 aggacaacta atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc
    31381 gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg cgccagacca
    31441 tcggccatat gcactgccgc ctgctcatga cgaactaaaa catgatcgat accacccacg
    31501 gtatgcaatg catcgtaaat atcaaggact gcgcctccag gataaccgaa tacttgttta
    31561 acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc
    31621 tgcctcactg tttgatggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag
    31681 caattggcag ccagagacat aaacgcaggg taataagaat aaacaggagg aaagatgaaa
    31741 agaaacgata aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca
    31801 caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc ccacacattc
    31861 agaataaaat tctacattca ataaaaaatg cagaataaac cagacattca tgtctgacct
    31921 attctgcatt cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc
    31981 ctttatcgcg cccggcagct tcatgccatg agagataaca ggttctgctg ttttgtttta
    32041 acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg
    32101 gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc
    32161 cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg
    32221 cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg
    32281 ggcctttaat tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg
    32341 gcacgctggt aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct
    32401 gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat
    32461 ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc
    32521 tgcaaacaca aagatgaaat acacgttcac tactcgcggg ttcaaaacct gaaccaggca
    32581 attcattttg tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag
    32641 cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca
    32701 ggcgtgcaac agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg
    32761 taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc
    32821 gtagctgtgg cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac
    32881 ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg
    32941 aagaatatga tttatc
//
LOCUS       DADLTD010000022        32459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLTD010000022 DADLTD010000000
VERSION     DADLTD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.022"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(392..598)
                     /locus_tag="JN701_000834"
     CDS             complement(392..598)
                     /locus_tag="JN701_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY7363363.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            911..1000
                     /gene="kdpF"
                     /locus_tag="JN701_000835"
     CDS             911..1000
                     /gene="kdpF"
                     /locus_tag="JN701_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY7363364.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1000..2673
                     /gene="kdpA"
                     /locus_tag="JN701_000836"
     CDS             1000..2673
                     /gene="kdpA"
                     /locus_tag="JN701_000836"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY7363365.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMCAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2696..4744
                     /gene="kdpB"
                     /locus_tag="JN701_000837"
     CDS             2696..4744
                     /gene="kdpB"
                     /locus_tag="JN701_000837"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAY7363366.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4753..5296
                     /gene="kdpC"
                     /locus_tag="JN701_000838"
                     /pseudo
     CDS             4753..5296
                     /gene="kdpC"
                     /locus_tag="JN701_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5289..7973
                     /gene="kdbD"
                     /locus_tag="JN701_000839"
     CDS             5289..7973
                     /gene="kdbD"
                     /locus_tag="JN701_000839"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAY7363367.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7970..8647
                     /gene="kdpE"
                     /locus_tag="JN701_000840"
     CDS             7970..8647
                     /gene="kdpE"
                     /locus_tag="JN701_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY7363368.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8863..9120)
                     /locus_tag="JN701_000841"
     CDS             complement(8863..9120)
                     /locus_tag="JN701_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363369.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9243..11441
                     /gene="speF"
                     /locus_tag="JN701_000842"
     CDS             9243..11441
                     /gene="speF"
                     /locus_tag="JN701_000842"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAY7363370.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGFDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11438..12776
                     /gene="potE"
                     /locus_tag="JN701_000843"
                     /pseudo
     CDS             11438..12776
                     /gene="potE"
                     /locus_tag="JN701_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
     gene            12804..13001
                     /locus_tag="JN701_000844"
                     /pseudo
     CDS             12804..13001
                     /locus_tag="JN701_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405579.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13125..13775
                     /locus_tag="JN701_000845"
     CDS             13125..13775
                     /locus_tag="JN701_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HAY7363371.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(13816..14310)
                     /locus_tag="JN701_000846"
     CDS             complement(13816..14310)
                     /locus_tag="JN701_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363372.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(14525..16165)
                     /gene="pgm"
                     /locus_tag="JN701_000847"
     CDS             complement(14525..16165)
                     /gene="pgm"
                     /locus_tag="JN701_000847"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY7363373.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFGFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(16191..16736)
                     /gene="seqA"
                     /locus_tag="JN701_000848"
     CDS             complement(16191..16736)
                     /gene="seqA"
                     /locus_tag="JN701_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY7363374.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIAEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            16921..17685
                     /gene="ybfF"
                     /locus_tag="JN701_000849"
     CDS             16921..17685
                     /gene="ybfF"
                     /locus_tag="JN701_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7363375.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            17756..18118
                     /gene="ybfE"
                     /locus_tag="JN701_000850"
     CDS             17756..18118
                     /gene="ybfE"
                     /locus_tag="JN701_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY7363376.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            18258..18788
                     /gene="fldA"
                     /locus_tag="JN701_000851"
     CDS             18258..18788
                     /gene="fldA"
                     /locus_tag="JN701_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY7363377.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            18998..19084
                     /gene="uof"
                     /locus_tag="JN701_000852"
     CDS             18998..19084
                     /gene="uof"
                     /locus_tag="JN701_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY7363378.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            19077..19523
                     /gene="fur"
                     /locus_tag="JN701_000853"
     CDS             19077..19523
                     /gene="fur"
                     /locus_tag="JN701_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY7363379.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(19608..19934)
                     /locus_tag="JN701_000854"
     CDS             complement(19608..19934)
                     /locus_tag="JN701_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363380.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(19984..21390)
                     /gene="chiP"
                     /locus_tag="JN701_000855"
     CDS             complement(19984..21390)
                     /gene="chiP"
                     /locus_tag="JN701_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY7363381.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(21837..23501)
                     /gene="glnS"
                     /locus_tag="JN701_000856"
     CDS             complement(21837..23501)
                     /gene="glnS"
                     /locus_tag="JN701_000856"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY7363382.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(23704..25650)
                     /gene="nagE"
                     /locus_tag="JN701_000857"
     CDS             complement(23704..25650)
                     /gene="nagE"
                     /locus_tag="JN701_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY7363383.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            25983..26783
                     /gene="nagB"
                     /locus_tag="JN701_000858"
     CDS             25983..26783
                     /gene="nagB"
                     /locus_tag="JN701_000858"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7363384.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            26843..27991
                     /gene="nagA"
                     /locus_tag="JN701_000859"
     CDS             26843..27991
                     /gene="nagA"
                     /locus_tag="JN701_000859"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7363385.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            28000..29220
                     /gene="nagC"
                     /locus_tag="JN701_000860"
                     /gene_synonym="nagR"
     CDS             28000..29220
                     /gene="nagC"
                     /locus_tag="JN701_000860"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY7363386.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            29268..30020
                     /gene="nagD"
                     /locus_tag="JN701_000861"
     CDS             29268..30020
                     /gene="nagD"
                     /locus_tag="JN701_000861"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAY7363387.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            30417..32081
                     /gene="asnB"
                     /locus_tag="JN701_000862"
     CDS             30417..32081
                     /gene="asnB"
                     /locus_tag="JN701_000862"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY7363388.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         7753 a         8197 c         8409 g         8100 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcaccccg ccggggcaca ctgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atttctcatc cttgttttat cgggttattt
      181 gtccgctgac aggcaaaacc ctacagattt ataagaaagc cttaagtgag atatccatca
      241 gatagaaaca attaggaaaa tatttatata tttttcctga atatatttca gctgggtaat
      301 accatatcta aatatttacc tgaacatctt cctaataaaa aaaccgccag catctccact
      361 agcggttttc aatgtattca aaactcggca atcagtaaaa atcaccggtt gccttttcgg
      421 cctgttccat ccaaaccggt ttatcgctgg ttttcgacca gacgcggtgc aggtagctgt
      481 aaaagcgggc gcggtctttc cagaaaagca tgaaaggcag cgccagaacg cccgctgcaa
      541 ccgcatatgt acggcgtaaa aagataagcc atgcaggata ttctctgtag agttccatac
      601 ttttctcccc cttttgtatc tacccggtga atagcaccgg aaaaatgaat ttgtttatct
      661 gatgaaaata gtaccgcctt ttgtgtaatt ttactactca tccgaccact tatttttgct
      721 tattgatggt ttatttacat tcactctgta attaagttgc acaaaagtta aatcaatgct
      781 aaacattagt taaaccatgg cttttgccat ttttatactt tttttacacc ccgcccgcag
      841 atttttgcga aatctttgca gccagaattc tactcttccg gtatcacttt taggccactg
      901 gaggtgcact gtgagtgcag gcgtgatagc cggcgtgttg ctggtgtttt tattactggg
      961 ttatttggtt tatgccctga tcaatgcgga ggcgttctga tggctgcgca agggttctta
     1021 ctgattgcca cgtttttact ggtattaatg gtgctggcgc gtcctttagg cagcgggctg
     1081 gcgcggctga ttaatgacat tcctcttcca ggtacagcgg gcgttgaacg tatactcttt
     1141 cgtttacctg gcgtctctga ccatgagatg aactggaagc aatatctttg tgccattctc
     1201 ggcctgaaca tgctggggct ggcggtgctg ttttttatgt tgctcggtca gcactatctg
     1261 ccgcttaatc cacagcagtt gccggggctg tcgtgggatc tggcgctgaa taccgccgtc
     1321 agctttgtca cgaataccaa ctggcaatct tatagcggtg aaaccacgtt gagctatttc
     1381 agccagatgg caggcttaac ggtacaaaac tttctttctg ccgccagcgg gattgcggtg
     1441 atttttgcct tcatccgtgc gtttactcgc cagagcatga gcacactcgg gaatgcctgg
     1501 gtcgatctgc tacgcatcac gttatgggtg ctagtccctg tggcgttgtt gatcgcactg
     1561 ttttttattc aacaaggtgc gctgcaaaac tttctgccct atcaggcagt gaataccgtt
     1621 gaaggagcgc aacagctgtt acccatggga ccagtagctt ctcaggaagc gatcaagatg
     1681 ctcggtacta acggcggtgg cttctttaat gccaactcgt cgcatccgtt tgaaaaccca
     1741 accgcactga ccaacttcgt gcagatgctg gcgatcttct tgatcccaac agctctgtgc
     1801 tttgcctttg gtgaagtgac gggcgatcgc cgccaggggc gcatgttgct gtgggcgatg
     1861 tcagtgattt ttgtcatctg cgtaggcgtg gttatgtgcg cagaagttca gggtaatcct
     1921 catctgctgg cgctcggcgc agacagcagc atcaatatgg aaggtaaaga gagccgtttc
     1981 ggcgtgctgg tcagtagcct gtttgcggtc gtgactacgg cggcgtcctg tggcgcggtg
     2041 attgcgatgc atgattcgtt taccgctctc ggcggcatgg tgccgatgtg gctaatgcaa
     2101 attggtgaag tggtgttcgg cggtgtcggt tctggtcttt acggcatgat gctgtttgtc
     2161 ctgctggcgg tgtttattgc cgggctgatg attggtcgta caccggaata tctgggtaaa
     2221 aaaatcgacg tacgcgagat gaaactgact gcactggcga ttctggttac tccaacactg
     2281 gtactgatgg gcgcggcgtt ggcgatgatg accgacgccg gacgtagcgc catgcttaac
     2341 cctggcccgc atggttttag cgaagtgctg tacgccgtgt cgtccgccgc taacaacaac
     2401 ggcagcgcct ttgccggatt aagcgccaac tctccgttct ggaactgttt actggcgttc
     2461 tgcatgtttg tcggtcgctt cggggtgatt atcccggtga tggcaattgc cggttcgctg
     2521 gtgagtaaaa agagccaacc cgccagctcc ggcacgctgc caacgcacgg cccgctgttt
     2581 gttggcctgt taatcggcac cgtgttgctg gttggcgcac tgacctttat ccctgccctg
     2641 gcgcttggtc cggtggcgga atatctctcc tgatgatatt gagtgagcac tgaatatgag
     2701 tcgtaaacaa ctggcgctat tcgaaccaac acttgtcgtt caggcgctga aagaagcggt
     2761 gaaaaaatta aacccgcagg cgcaatggcg caatccggtg atgtttatcg tctggatcgg
     2821 cagtctgctg accacctgta ttagcatcgc gatggcaagc ggtgcgatgc ctggcaatgc
     2881 gctgtttagc gcggcaatta gcggctggct gtgggtcacc gtactgttcg ctaatttcgc
     2941 cgaggcgctg gcagaaggcc gcagtaaagc gcaggccaac agtcttaaag gggtgaaaaa
     3001 aactgccttt gcccgcaagc tgcgtgagcc gaaatatggc gctgcggcgg acaaagttcc
     3061 tgccgatcaa ctgcgtaaag gtgacatcgt gctggtagaa gctggcgata ttatcccctg
     3121 cgatggtgaa gtcattgaag gcggcgcatc ggtcgatgaa agcgccatca ccggggaatc
     3181 ggcaccggtg atccgtgaat ccggcggcga ttttgcctcc gtcaccggcg gcacgcgtat
     3241 tctttctgac tggctggtga ttgagtgtag cgttaacccc ggtgagacat ttctggatcg
     3301 gatgatcgcg atggtggaag gcgcacagcg acgcaaaaca ccgaacgaga ttgccctgac
     3361 tatcctgctg attgctctga ccatcgtctt tttactggca accgccacgc tgtggccgtt
     3421 ttccgcgtgg ggcggtaatg ccgtgagtgt aacggtactg gtggcgctgc tggtctgtct
     3481 gatcccaacc actattggcg gcctgttgtc agcgatcggc gtcgccggga tgagtcggat
     3541 gctgggcgcg aatgtgattg ccaccagcgg acgtgcagtt gaagcggcag gtgacgttga
     3601 cgttctgcta ctggataaaa ccggcaccat cacactcggt aaccgtcagg cgtcggagtt
     3661 tatccctgcg cagggcgtgg atgaaaaaac gctggctgac gccgcacaac tggcttcgct
     3721 ggctgatgaa acgccggaag gccgcagtat tgtgatcctc gccaagcaac gttttaacct
     3781 gcgcgagcgc gatgtgcagt cgctccatgc cacctttgta ccgtttactg cgcaaagccg
     3841 gatgagcggg atcaacatcg acaaccgcat gatccgtaaa ggttctgtcg atgccattcg
     3901 ccgccatgtt gaggctaacg gcggtcactt ccctgccgat gttgatcaaa aagtcgatca
     3961 ggttgcgcgt cagggggcca cgccgctggt ggtggtggaa ggttcccgtg tgctgggcgt
     4021 tattgcgctg aaagatatcg tcaaaggcgg tattaaagag cgcttcgccc agctgcgcaa
     4081 aatgggcatt aaaacggtga tgattaccgg cgataaccgt ctgactgccg ccgccattgc
     4141 tgcggaagcg ggcgtcgatg attttctcgc cgaagcgaca ccggaggcca agctggcatt
     4201 gattcgtcag tatcaggcgg aaggccgttt ggtggcgatg accggcgacg gcaccaacga
     4261 tgctccggcg ctggcgcaag cagatatcgc ggtggcgatg aactccggca cccaggcggc
     4321 gaaagaggcg ggcaatatgg tcgatctcga ctctaacccg accaagttga tcgaggtggt
     4381 gcatattggc aaacagatgc tgatgacccg tggctcgctg accaccttca gcattaccaa
     4441 cgatgtggcg aaatacttcg ccattattcc ggcggcgttc gcggcaacgt atccgcagtt
     4501 aaatgcgctg aacatcatgc gcctgcattc gcccgactcc gccattctca gtgcagtcat
     4561 tttcaacgcc ttgattatcg tctttttgat tcctctggcg ttaaaaggcg tgagttataa
     4621 accgcttacc gcttctgcca tgttgcgccg taacttatgg atttacggtc tgggtgggct
     4681 gctggtgccg tttattggta tcaaagtcat tgatttactg ctgaccgttt gcggtctggt
     4741 gtgaggttta ccatgcgtgg cttacgtccg gcattatcga catttatctt tctgttattg
     4801 attactggcg gcgtttaccc gctgctgacc accgtgctgg ggcaatggtg gtttccctgg
     4861 caggccaacg gttcgttgat tcgtgaaggt gatacggtgc ggggttcggc attaatcggg
     4921 cagaatttta ccggcaacgg cagaaatgcc ctataatcca caggcttctg gcgggagcaa
     4981 tctggcggtc agtaaccctg agctggataa acaaatagcc gcacgcgttg ctgcattacg
     5041 ggccgctaac ccggatgcca gtacgaacgt tccggttgaa ctggtgacgg catcggcaag
     5101 cgggctggac aataatatca ccccgcaagc ggcggcctgg cagatcccac gcatcgcgaa
     5161 agcgcgtaat ctcagcgttg aacagctcac gcagctgatc gcaaaataca gccaacaacc
     5221 gctggtgaaa tatatcggcc agccggtcgt caacattgtc gaactcaatc tggcgctgga
     5281 taagcttgat gaataacgaa cccttacgtc ccgaccccga tcgtctgctg gaacaaactg
     5341 ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc ctgtgcaggc gtcgggaaga
     5401 cctgggcgat gctggcagaa gcccagcgac tgcgggcgca agggctggat attgtggttg
     5461 gcgtggtaga aacccacggg cgaaaagata ccgacgcaat gctggaaggg ctggctgttc
     5521 tgccgccaaa acgcctggcg taccgtgggc ggcacatctg cgagtttgac ctcgatgccg
     5581 ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt aatgcgctag
     5641 gttctcgtca ccctaaacgc tggcaggaca tcgaagagct gctggaagca ggcattgatg
     5701 ttttcactac cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca
     5761 ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc gacgacgtgg
     5821 ttctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc aaagtctata
     5881 ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc
     5941 tgcgcgaact ggcactgcgc cgtactgccg atcgcgttga tgagcaaatg cgcgcctggc
     6001 gggggcatcc tggcgaagag aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg
     6061 gccataacac cggtagtgag aagctggtcc gcgcggcggc gcggctggcg tcacggctgg
     6121 gtagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcaccgctta ccggaaaaaa
     6181 aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg gagacggcaa
     6241 cactttctga tccagcggaa gaaaaagcag tagtgcgtta tgcccgtgaa cataatctcg
     6301 gcaagattat tctcggtcgc ccggcctcgc gccgctggtg gcgtcgggaa acgtttgctg
     6361 accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac
     6421 ccgcccgcac gattaacaac gcgccggata gccgctcttt taaagacaag tggcgggtac
     6481 agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacttta attgccatgc
     6541 agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg
     6601 tggtggcgct attttatgga cgctggcctt cggtggttgc taccgtcatt aatgtggtga
     6661 gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc
     6721 tgctgacctt cgcagtgatg ttaaccgtcg ggctggtgat cgggaacctt actgctggcg
     6781 tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac ttatatgaaa
     6841 tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc atcgaacaat
     6901 ttattgcctc cacgtttcat gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat
     6961 tgcagccgtt aacacatccg caaggaatga cgccatggga cgataccatc gcgcagtgga
     7021 gttttgataa aggcctgcct gcgggcgcgg gcaccgacac gttacccggt gtaccgtacc
     7081 agattttgcc gctaaaaagc ggcgagaaaa cctacgggct ggtggtggtg gagccgggga
     7141 atctgcgcca gttgatgatc ccggaacagc agcgcctgct ggagacgttt acgctgttag
     7201 tcgccaatgc ccttgagcgg ctgacgctaa ccgccagcga agaacaggcg cggatggcaa
     7261 gcgaacgtga acagatccgc aacgccctac tggcagcgct ttcgcatgat ttacgcacgc
     7321 cgctcacggt gctgttcggt caggcagaaa tcttaacgct cgatctggca agcgaaggat
     7381 cgccccacgc ccgccaggcc agcgagattc gtcagcatgt gctgaacact acccgactgg
     7441 tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg aagaaagagt
     7501 ggttaacgct ggaagaagtg gtcggtagcg cgctgcaaat gctggaaccg ggtttatcgt
     7561 cgcccatcaa tctttctctg ccagaaccgc tgaccttaat ccacgttgac gggccactct
     7621 ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg
     7681 aaattggtat cgatgcccac gttgagggcg aaaatctaca actggatgtc tgggataacg
     7741 gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct cgcgggaata
     7801 aagagtcggc agtaccgggg gtagggcttg gactggcaat ttgtcgggcg atagtggatg
     7861 tacacggggg cactattacc gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta
     7921 cacttcccca gcaaactgcc cctgaacttg aagaatttca tgaggatatg tgacaaacgt
     7981 tctgattgtt gaagatgaac aggctattcg tcgctttctg cgcacggcgc tggagggcga
     8041 cgggatgcgc gtctatgagg ccgaaacgct gcaacgcggc ttgctggaag cggctacccg
     8101 taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatggga ttgagtttat
     8161 ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg ctttccgcac gcagcgaaga
     8221 gagcgacaaa atcgccgcgc tggatgccgg agcggatgat tatctgagta agccgtttgg
     8281 cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgc cgccactccg ctaccgccgc
     8341 gcccgatccg ctggtgaaat tttccgatgt taccgtcgat ttagccgccc gcgtgattca
     8401 ccggggtgag gaagaggtgc atctcacacc aattgagttc cacctgctgg cggtgctgct
     8461 caacaatgcc ggaaaagtac tcacccagcg ccagctcctt aaccaggtgt gggggccaaa
     8521 cgcggtcgaa cacagtcact atttgcgtat ttatatggga catctgcgac aaaaactgga
     8581 acaggatccc gcccgcccac gccatttcat tactgaaacc ggtattggct atcggtttat
     8641 gctttgaata ttaattttaa tacagcctgc cttttattaa ttaaagccgt aataataaat
     8701 acggcttttt atcttaaata acacacaaaa ataacaattc aatattttat attactgagt
     8761 aaaaagctca tcattaaata aattaagatt gatcattttt tattgatcac cttcacagtt
     8821 caaccgtatt tcctggatag aatatcttca ccttcattca catcaggaaa ggtaaattaa
     8881 atggaaaata acagccgcac tatgccccat ataaggcgga caactcatat tatgaagttt
     8941 gctcatcgca atagcttcga ctttcacttc ttcaatgccc gttagtctac cgactaaggg
     9001 cacttcagcg tacaggtctt cctgactctc tgtattacag gcatatgcct gtattccgca
     9061 gtgctcattc tctgttaacc cttgtattat cagccaccgg actggtaagg gtttctgcat
     9121 tctgaaaaaa gcaatctacc tgatttattt atcagcgggg taactttgct tttttccggc
     9181 acgatcgatt tctcattcga gaaattgagg acctgctatt acctaaaata aagagatgaa
     9241 aaatgtcaaa attaaaaatt gcggttagtg attcttgccc ggactgtttt accacgcagc
     9301 gagaatgtat ctacattaat gaaagtcgta atatcgatgt ggcggcaatc gttttatcgc
     9361 tcaacgatgt tacatgcgga aaactcgatg aaatcgatgc cacgggttat ggcatcccgg
     9421 tatttattgc tactgaaaat caagaacgtg tacccgcaga gtatttgccc tgtatttcgg
     9481 gtgtctttga gaattgcgaa tcgcgacgag aattttatgg tcgtcagtta gaaaccgctg
     9541 ccagccatta tgaaactcaa ctgcgcccac ctttcttccg cgcactggtc gattatgtca
     9601 atcaaggtaa cagcgcgttt gattgccctg gtcatcaggg cggcgaattt ttccgtcgcc
     9661 atccggcggg gaatcagttt gtggaatact ttggtgaggc gctgttccgt tccgacttgt
     9721 gcaacgcaga cgtagcaatg ggtgatctgc tgattcacga aggcgcgcca tgcattgcac
     9781 agcaacatgc ggcaaaagtg ttcaatgccg ataaaacata cttcgttcta aatggcactt
     9841 catcttctaa caaagtggtt ttaaacgccc tgctgacacc gggtgatctg gtgctgtttg
     9901 accgcaataa ccacaaatct aaccaccacg gagcgttgct acaggctggt gcaacaccgg
     9961 tttatctgga aaccgcacgt aacccgtatg gctttatcgg tggcattgat gcgcactgtt
    10021 ttgaagaaag ttacctgcgt gagctgatcg cggaagtcgc accgcagcgg acaaaagagg
    10081 ctcgtccttt ccgcctcgcc gttattcagc taggcaccta cgacggtacg atttataacg
    10141 cccgccaggt ggtggataaa attggtcatc tgtgtgacta cattctgttt gactccgcat
    10201 gggtcggcta tgagcaattt atcccgatga tggctgactg ttcaccgctg ttgctggatc
    10261 ttaatgagaa cgatccgggc attctggtta cgcaatctgt gcataaacag caggctggtt
    10321 tttctcaaac ttcacaaatc cataaaaaag acagccacat caaagggcaa cagcgttatg
    10381 taccgcacaa acgcatgaac aacgccttta tgatgcatgc ctccaccagc ccgttctatc
    10441 cgctgtttgc cgcactggat atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata
    10501 tgtggatgga ttgtgtggta aatggcatta atgcccgcaa gctgatcctc gataactgtc
    10561 agcatattcg tccgttcgta cctgaactag tggatggtaa accctggcag tcgtatgaaa
    10621 cagcgcaaat tgcggttgat ctgcgcttct tccagtttgt accaggggaa cactggcact
    10681 cttttgaagg ctatgcagag aatcaatact ttgtcgatcc atgcaaactg ttgctgacaa
    10741 ccccaggttt tgatgcacgt aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc
    10801 ttgctaactt cctgcgcgaa aatggcgtag tgccggaaaa atgcgatctt aactccatcc
    10861 tcttcctgct gactccggca gaagatatgg ccaaacttca gcaacttgtt gccctgctgg
    10921 tacgcttcga aaaactgctt gagtccgacg cgccattagc agaagtgctg ccttccatct
    10981 acaaacagca tgaagagcgc tacgccggtt atacccttcg tcagttatgc caggaaatgc
    11041 atgatttgta tgcccgtcac aacgtgaaac aaccgcaaaa agagatgttc cgtaaggaac
    11101 acttcccacg cgtaagcatg aatccacaag aagccaacta cgcctattca cgcggtgaag
    11161 tggagctggt tcgtctgccg gatgccgaag gccgtatcgc tgccgaaggt gcgcttcctt
    11221 atcctccggg tgtgctgtgt gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt
    11281 acttcagcgc tctggaagaa gggatcaacc tgctgccagg ttttgcaccg gagctgcagg
    11341 gtgtctatat cgaagaacat gatggtcgta agcaagtttg gtgctatgtc atcaagcctc
    11401 gtgatgcgca aagcaccctg ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa
    11461 aatgggcgtc gttcagttaa ccatactgac gatggtcaac atgatgggct ccggtatcat
    11521 catgctgccg acaaagcttg ccgaagttgg gacaatctca attatctcct ggctggtgac
    11581 agccgtaggc tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa
    11641 atcaggcggt atgggcggtt atgcggagta tgctttcggt aagtccggca attttatggc
    11701 gaactacacc tacggtgtct cgctgctgat tgctaacgtc gcgattgcta tttcggcggt
    11761 tggttacggc accgaactgc tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac
    11821 catcggagtg ctgtggattt gtaccgtggc taactttggt ggtgcgcgca ttaccgggca
    11881 aatcagtagc attactgtgt ggggagtcat tattccggtc gtcggtctgt gcattattgg
    11941 ctggttctgg tttagtccga cgctgtacgc taactcatgg aacccgcacc atgtgccgtt
    12001 ctttagcgct gtaggttctt ccatcgccat gacgttgtgg gctttcctcg gtctggagtc
    12061 agcctgtgcc aacgccgaag tagtggaaaa cccggaacgt aacgtgccga ttgcagttct
    12121 tggcggtact ttaggtgcgg cggtgattta tatcgtctcc accaacgtga ttgccgggat
    12181 tgtgcctaat atggagctgg caaattcaac ggcaccattt ggcctggctt tcgcacagat
    12241 gttcacacca gaagtgggta aagtcattat ggcgctgatg gtgatgtcct gctgcggttc
    12301 actgcttggc tggcggttca ctgcttggct ggcagttcac cattgcccag gtgtttaaat
    12361 cttcatctga tgaaggctac ttccctaaaa ttttctcccg tgtaaccaaa gtggatgcac
    12421 cggtgcaggg aatgttgacc attgtgatta ttcagagtgg attggcactg atgaccatta
    12481 gcccgtcact gaacagccag ttcaacgtgc tggttaacct ggccgtggtg accaatatca
    12541 ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct aatgtgcccc
    12601 cctcaaaagc gaaagtagca aactttgttg cctttgttgg tgcgatgtac agtttctatg
    12661 cgctgtactc atccggggaa gaagccatgc tgtacggttc tatcgtgacc ttccttggtt
    12721 ggacactgta tggtctggta tcaccatgct ttgaactgaa aaataaacac ggttaataaa
    12781 acaaagtaaa aaagggcgat tgaatgatcg ccctttctgt ttgttggcaa atagttcgtt
    12841 atttatgccg catccggcag atgtagggtc taaacgcgat cgaacacagc aactatttgc
    12901 gtaattatat ggggcatctg cgacaaaaac tggaacagga tccggcccgg cctcgccatt
    12961 ttattactga aaccggtatt gggtatcggt ttatgctttg aaaaaagtgg caatgtatgg
    13021 ataaagtcat tttttattca aatataagga acttaatatt taaaaaatgt tccatacaat
    13081 tcctcatcat aaatgacacc gtagcacaca aggagcaagt agcgatgcgt gaaatgaatc
    13141 agttagtaaa tactgaagat tcagcctggc caataatcca gaactggtta aaagacgcca
    13201 caaaccatac cgaactctta ccggttaata aaaatttagc cgaaaccgca ctttatcaat
    13261 tacaggtgac aacaaaatca ccgatgggtg cgcttgtcta tggctccgga ggtctgctga
    13321 ttgataatgg ttggttacgc atagcaggat ctggtcaccc ccggttgcca cgagatccag
    13381 taagttggac acaacgccct gaattcgcag gcgtgcgggc tttacccatc gctgacgatg
    13441 tcgcaggggg aatttttgct cttaatggtg gcgatcttgg ggaagatact ggctgcgttt
    13501 attattttgc gccagatacg ctgaattggg aaagtctgga agttggatat tctgagtttc
    13561 ttcagtgggc attatccggt gatttagata ccttctatga aaatgtacga tggcagcaat
    13621 ggcgagaaga cgtaataaaa ttatcggcaa cggaagcctt cacgttctac cctttcttat
    13681 gggtacaaag tgaagaagca agaacaagaa aagttatttc gcttactgaa ctctgggaaa
    13741 tgcagtatca aatgaaagaa acattcacgc aataagacat gttcaggtcc gggtttcatt
    13801 cccggactca ctattttaat gtgtatttat cgctttcaat tcttgctggc aattttctga
    13861 ctggcagcgt cgtaactcaa taatcccgac atgcatcatc gagccaaaaa atccgctttc
    13921 gctgacataa tcctcattga ctatcggtcg agtgccgggc ttgccctggc aaacccgcat
    13981 aaaaaatgag gtgtagcatt gccaaccggg atttaaaccg accttttctt gtgctgacat
    14041 attatgaaga taagtaagtt ctttttcgtt cccgcctgac aactgccagc ggcgggtatt
    14101 atcaggtaag ttatcaccac ggcaatttgt cttacaccgg gaaggtgcaa tatttaccgc
    14161 aatgctaccc gttaatcttc tggttttatc tttaggggtc agtaaccagt cgccagctaa
    14221 tgatgattgt tcaggttggg cgcatgcagt cagcagtaat gtggcggtaa ttagtgacac
    14281 tatgacgacc agactcctgt tggtcttcat tgggatttcc ttgagctact cagtttgttg
    14341 acaaagtgtt cgttgttttt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    14401 tcgtaaattc aatatattgc accatcctcg taggcctgat aagcgtagcg catcaggcaa
    14461 ttctgtgttt gtcatacgta aaaaagggcg atcttccgac cgcccttttt cttatttaat
    14521 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    14581 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    14641 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg atagaagcac
    14701 cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc
    14761 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    14821 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    14881 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg atgccgtctt
    14941 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    15001 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggga
    15061 cttctaccag cttgcggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    15121 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    15181 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    15241 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt gccagcaggc
    15301 ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta tcaaggtgca
    15361 taaagccgaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    15421 cgccaatacg cttccagtat tcgataccgg aaccgcccag cggatcaacg cccagcgtca
    15481 ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggc cagaccttcc acaaacggct
    15541 gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg gagatacgct
    15601 tcacgccttt caggccatcg gccagcagtg cgttggccct gtcttccacc actttggtga
    15661 cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    15721 ggttatggga cggtgtaatc acgataccgt ctgccagcgg accacctttt ttattgtgaa
    15781 ccaggatggc attggaaacg gcaggcgttg gagtgaagcc attgttttcc tgcacaatga
    15841 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    15901 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    15961 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    16021 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    16081 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    16141 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    16201 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    16261 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    16321 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtaa
    16381 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    16441 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    16501 tcagaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    16561 gcttcggcga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    16621 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    16681 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    16741 atccagtgca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    16801 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    16861 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    16921 atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    16981 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    17041 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagatccg
    17101 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc acagcagatc
    17161 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttacggca
    17221 ctggcccgcg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    17281 gtacgccgtc atgatgagat tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa
    17341 actcgccagc aagcagcagc aataatgcgc cagcatctta atgaagaagg ggtgattcag
    17401 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    17461 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    17521 atccctagcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    17581 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    17641 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    17701 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    17761 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    17821 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    17881 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    17941 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    18001 ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc gtagcgaact
    18061 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tagtttaaat
    18121 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg aaagactgcc
    18181 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    18241 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    18301 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    18361 acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg ctgggcatcc
    18421 caacctggta ttatggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    18481 agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    18541 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    18601 cgatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggctg
    18661 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    18721 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctca
    18781 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    18841 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc
    18901 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    18961 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    19021 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    19081 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    19141 tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    19201 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    19261 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    19321 tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag gttatcgaat
    19381 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    19441 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    19501 aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa aaagccaacc tgcgggttgg
    19561 cttttttatg caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
    19621 ctgcgaattt cctgccaaac tttatcacaa tcggctttca ccgcctgatc atttccggtt
    19681 tgcgttgcac gcagacactg ctgataatcc agtacgcgga cactttcctg gtcggcaaaa
    19741 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
    19801 tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag acgactgtct
    19861 tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat ggcgatgaga
    19921 atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg
    19981 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    20041 gatgttgccg taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    20101 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    20161 gctgtatgca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    20221 ccaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg
    20281 aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttagc
    20341 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggc
    20401 tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc aggtgccttc
    20461 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    20521 ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa
    20581 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    20641 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    20701 gaagtcgtat ttcgccccaa gggagtgcag ataatcaact ttggtggttt tatcgttctg
    20761 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    20821 gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    20881 agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg gctgaatgta
    20941 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    21001 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    21061 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acacagcaat
    21121 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    21181 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    21241 acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    21301 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag
    21361 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    21421 gcaccaaagg tgaacagcga aaaaaataaa aattaaaaaa gtagcattga gaaaagtctc
    21481 gctgactaca ggaatagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca
    21541 gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt
    21601 tgcggttgaa aggcttgcag aagaataaaa gcgtaaattt ccagcaagat gaaagcggtt
    21661 acattttatt taattatggt ttgtgatttt aaatttattt tatggataaa ttgcgaagcg
    21721 gaattaattc gcgaagaaaa gcagaaaaaa cgccgcagag tgcggcgttt gggattgttt
    21781 gctatttctt gccggatgcg acgtaaatgc ctcacccggc ctggtagaac ttaaaattac
    21841 tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcc
    21901 gctgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta
    21961 cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc
    22021 gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct gaacagacgg
    22081 tcgtacagac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct
    22141 ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag
    22201 atggtggtga tattgccttc ggcatctttc tccacgcgtt ctgccttaat gacgtaagca
    22261 ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg
    22321 cggaaatcgg cgcgatcgat ccaaatctca ctgctaaacg gcacctggcg gctacccatt
    22381 tccggtttgt tcggatggtt cggcatggta accatttcgc cttcgccctg atagttttcg
    22441 ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gggcgttttc gttgagatct
    22501 tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgcct ggtcacgccg
    22561 atgcgtttgc agaactcacg aatagaagca gcagtgtaac cacgacgacg cagaccggaa
    22621 atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc
    22681 agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc
    22741 gggtgaacag gaatggtgat gttgtccagc acccagtcgt acaggcgacg gttgtcctgg
    22801 aactcaagcg tacacagaga gtgcgtaata ccttccaggg catcgctgat gcagtgggtg
    22861 aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta
    22921 atgcggtaca gcaccggatc gcgcatcacg ataaacggtg aagccatgtc gattttcgca
    22981 cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccagg
    23041 ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt cagggtgccg
    23101 cggtattcgc ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc tttattgatc
    23161 agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca
    23221 gaccagtgaa aacctaacca ctctacgtcg tttttgatcg actcaacata ctcgatatct
    23281 tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg
    23341 atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc
    23401 ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc
    23461 tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt
    23521 aaacaacgta taacggcgta tgatcttata agcggaacag gctgacaact gttagttttt
    23581 taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga
    23641 agatctccac cgcttgtcat tgttggatgc gacgctcaag cgtcgcatca ggcataaagc
    23701 agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt
    23761 aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg
    23821 ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg
    23881 cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc
    23941 catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt
    24001 gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac
    24061 cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc
    24121 cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc
    24181 ctgcggtttt gctacaggcg cggcagttga cggagttgct tcagctgacg cagcggctac
    24241 cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac
    24301 aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca
    24361 catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg tgatacaggc
    24421 gtcaattgct ttcaggttgt cagtgccgcc aaccgcagca atatagttgg tcgccagttg
    24481 attgaggcct tcttcagtgt tgctgttggc ttcttcagta acgatctcat cttctttatc
    24541 ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta
    24601 gatagcgaag aagacaacac ccatcaccag cagcatccag acgttctggc tggcggccgg
    24661 caggttatac atcaacgcgt agtcgatagc ccccgcagag aaagagaagc cagcatggat
    24721 acccagcagt gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag
    24781 cagcggtgca aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc
    24841 agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc
    24901 gaagtacatc gccagcgccg cacccggcag accgaacatc ataatcggga agaagccgga
    24961 catgaacatc cccgcggtgc cgtcaccggc atagaagcgg ttgatatcac cgtggaaaac
    25021 cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg
    25081 atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc
    25141 gcccgcagaa acgatccact cgcctcctgc atggatagcg tgctgtaccg gcggccagac
    25201 gtaaccaaaa atggccgcca gcaccaggca gaagaagccg gtggcaatcg gcacaaagcg
    25261 tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac ggttataggc
    25321 tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttta tttctgggtt
    25381 gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc
    25441 acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt
    25501 atcaaaaatc gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc
    25561 gaatcgcagc aacagtgccg ccaccggcag caccgcgata gggagctgta acgccctacc
    25621 gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc
    25681 tcgtttaaag ccgtattttt attttgctgc aaattgtact gccgatgttc tgtaatcaga
    25741 ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg
    25801 tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaac
    25861 cagaaaactt attttatgat tcaaaaaatc aggtcggatt gacgcctgtc tgcgcaaatc
    25921 caagttacgc ttaaagatgc ctaatccgcc aacggcttac attttactta ttgaggtgaa
    25981 taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata
    26041 tcgtcaatcg tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgtt ctgggcctgc
    26101 caactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc
    26161 aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag
    26221 agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc
    26281 cagcagaaaa catcaacctt ctcaacggca acgccccgga tatcgacgcc gaatgccgcc
    26341 agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta
    26401 acgacggcca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca
    26461 aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc
    26521 aggtgccaaa atatgccctg actgtcggtg ttggtacatt gctggatgcc gaagaagtga
    26581 tgattctggt gctgggtagc cagaaagcac tggcactgca ggctgcagtt gaaggttgcg
    26641 tgaaccacat gtggaccatc agctgtctgc aactgcatcc aaaagcgatc atggtgtgcg
    26701 atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag
    26761 cagaaaatat caaaggtctg taattgttat ccctgccctg gctccttgct cagggcaata
    26821 ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca
    26881 cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc
    26941 ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc
    27001 cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga
    27061 agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac
    27121 taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt
    27181 tatgcgcgag tacctggcaa gacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc
    27241 gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc
    27301 cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc
    27361 ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc
    27421 cggtcactcc aacgcgacgt tgaaagaagc gaaagccggt ttccgcgcgg ggattacctt
    27481 tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg
    27541 cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga
    27601 ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga
    27661 cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata
    27721 ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat
    27781 gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg
    27841 tatggcgacg ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg gcacactcgc
    27901 cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat
    27961 cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct
    28021 cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt
    28081 gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc
    28141 agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag
    28201 caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac
    28261 gcaatcggcg tacggcttgg tcgtcacgac gccaccatca ccctgtttga tctcagcagc
    28321 aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcaaac gctggaacat
    28381 gccctgttga atgccattgc tcagtttatt gatagctacc agcgcaaact gcgcgagctg
    28441 atcgcgattt cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac
    28501 atgccgcata ttcaggtaga aaactggggg ctggtagaag ctctggaaga acgttttaaa
    28561 gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt
    28621 gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg
    28681 attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga aattggccat
    28741 attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact
    28801 atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag
    28861 agccgcgtgc cgctggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc
    28921 gatagtctgg cgtcggaagt aattgagtat gtcggtcgtc atctgggtaa aaccatcgcc
    28981 attgctatca acttatttaa tccgcaaaaa attgttattg ccggtgaaat caccgaagcc
    29041 gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaggcgct gaaggcgttt
    29101 cgcactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt
    29161 gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa
    29221 tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa
    29281 atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag
    29341 cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc
    29401 cttcgcagac tgggcaagat ctggcgaacc gctttgccac cgcaggtgtc gatgtacctg
    29461 acagcgtgtt ttatacctct gcgatggcga ctgccgattt cctgcgtcgc caggaaggca
    29521 agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca
    29581 ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacgagttcc tacaactggg
    29641 acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgttttatc gccaccaatc
    29701 cggacaccca cgggcgcggt ttttatcccg cgtgtggcgc gttgtgtgca ggcatcgaaa
    29761 aaatctccgg gcgtaaaccg ttctatgttg gtaaaccgag tccgtggatc atccgcgcgg
    29821 cattaaacaa aatgcaggcg cattcggaag aaacggtgat tgtcggcgat aacctgcgta
    29881 ccgatattct ggcgggcttc caggcaggtc tggaaacgat tctggtgctt tctggtgttt
    29941 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcggtcg
    30001 ctgaaatcga cgttatctga atagcaacca cgtctgcgga cgtggttggc tctgatgaca
    30061 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
    30121 aattttcatg cttttgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaatt
    30181 gcactttgtc agcaatttgg cccccgtaaa atacaaacat tcaataaaac ccgctgaaaa
    30241 ttatcgattc atcaataaat atatcctcac accagacctt ttttcaccaa tcgacaatca
    30301 ccattacgtt ttttattttt tcaccgccaa atcgcttgcg cacagtgctg ctttgcggca
    30361 tttttttaaa caagcaaaca caacaagcaa caaataccag attaacggag aaggttatgt
    30421 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    30481 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
    30541 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    30601 ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc
    30661 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
    30721 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    30781 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
    30841 ggatcatccc actgtatatg ggctatgacg aacacggtca gctgtatgtg gcctcagaaa
    30901 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
    30961 ggagccagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
    31021 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc tctggaagat tccgttaaaa
    31081 gccatctgat gtctgatgtg ccttacggtg tgctgctttc tggtggtctg gattcctcaa
    31141 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
    31201 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
    31261 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    31321 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    31381 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    31441 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
    31501 ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc
    31561 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    31621 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    31681 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    31741 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    31801 tcgacaccct gaaagaagtg gcggcgcagc aggtttctaa tcagcaactg gaaactgccc
    31861 gcttccgctt cccgtacaac acgccaacct caaaagaagc gtatctgtac cgggagatct
    31921 ttgaagaact gttcccgctt ccgagcgccg ctgagtgcgt gcctggcggt ccttccgtcg
    31981 cgtgttcttc cgctaaagcg atcgaatggg atgaagcgtt caagaaaatg gacgatccat
    32041 ctggtcgtgc ggttggtgtt caccagtcgg catataagta agaaatgcga aacagccccg
    32101 gtgaaatccg gggctgtttc agttattgag gtgcttgttg attctgatat gcagtaagga
    32161 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    32221 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg
    32281 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
    32341 ttcgcaacct aaccaaatag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    32401 gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaaag
//
LOCUS       DADLTD010000023        31371 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLTD010000023 DADLTD010000000
VERSION     DADLTD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.023"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(15..3296)
                     /locus_tag="JN701_000863"
     CDS             complement(15..3296)
                     /locus_tag="JN701_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY7363389.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3414..4607
                     /locus_tag="JN701_000864"
     CDS             3414..4607
                     /locus_tag="JN701_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY7363390.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4671..5924)
                     /locus_tag="JN701_000865"
     CDS             complement(4671..5924)
                     /locus_tag="JN701_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY7363391.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6252..7442
                     /gene="hmpA"
                     /locus_tag="JN701_000866"
     CDS             6252..7442
                     /gene="hmpA"
                     /locus_tag="JN701_000866"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY7363392.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7487..7825)
                     /gene="glnB"
                     /locus_tag="JN701_000867"
     CDS             complement(7487..7825)
                     /gene="glnB"
                     /locus_tag="JN701_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY7363393.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7886..9220)
                     /gene="glrR"
                     /locus_tag="JN701_000868"
     CDS             complement(7886..9220)
                     /gene="glrR"
                     /locus_tag="JN701_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY7363394.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9210..9923)
                     /gene="qseG"
                     /locus_tag="JN701_000869"
     CDS             complement(9210..9923)
                     /gene="qseG"
                     /locus_tag="JN701_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY7363395.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10088..11515)
                     /gene="qseE"
                     /locus_tag="JN701_000870"
     CDS             complement(10088..11515)
                     /gene="qseE"
                     /locus_tag="JN701_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY7363396.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12073..15960)
                     /gene="purL"
                     /locus_tag="JN701_000871"
                     /gene_synonym="purI"
     CDS             complement(12073..15960)
                     /gene="purL"
                     /locus_tag="JN701_000871"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7363397.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16220..17776
                     /gene="mltF"
                     /locus_tag="JN701_000872"
                     /pseudo
     CDS             16220..17776
                     /gene="mltF"
                     /locus_tag="JN701_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
     gene            complement(17773..18309)
                     /gene="tadA"
                     /locus_tag="JN701_000873"
     CDS             complement(17773..18309)
                     /gene="tadA"
                     /locus_tag="JN701_000873"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY7363398.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18334..18969)
                     /gene="yfhb"
                     /locus_tag="JN701_000874"
     CDS             complement(18334..18969)
                     /gene="yfhb"
                     /locus_tag="JN701_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY7363399.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19178..20026
                     /locus_tag="JN701_000875"
     CDS             19178..20026
                     /locus_tag="JN701_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7363400.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20082..20342
                     /gene="yfhL"
                     /locus_tag="JN701_000876"
     CDS             20082..20342
                     /gene="yfhL"
                     /locus_tag="JN701_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY7363401.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20536..20616)
                     /gene="shoB"
                     /locus_tag="JN701_000877"
     CDS             complement(20536..20616)
                     /gene="shoB"
                     /locus_tag="JN701_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY7363402.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21036..21416)
                     /locus_tag="JN701_000878"
     CDS             complement(21036..21416)
                     /locus_tag="JN701_000878"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY7363403.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21416..22147)
                     /gene="pdxJ"
                     /locus_tag="JN701_000879"
     CDS             complement(21416..22147)
                     /gene="pdxJ"
                     /locus_tag="JN701_000879"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY7363404.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22159..22887)
                     /gene="recO"
                     /locus_tag="JN701_000880"
     CDS             complement(22159..22887)
                     /gene="recO"
                     /locus_tag="JN701_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY7363405.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22899..23804)
                     /gene="era"
                     /locus_tag="JN701_000881"
     CDS             complement(22899..23804)
                     /gene="era"
                     /locus_tag="JN701_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY7363406.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23801..24481)
                     /gene="rnc"
                     /locus_tag="JN701_000882"
     CDS             complement(23801..24481)
                     /gene="rnc"
                     /locus_tag="JN701_000882"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY7363407.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24754..25728)
                     /gene="lepB"
                     /locus_tag="JN701_000883"
     CDS             complement(24754..25728)
                     /gene="lepB"
                     /locus_tag="JN701_000883"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY7363408.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGMKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25744..27543)
                     /gene="lepA"
                     /locus_tag="JN701_000884"
     CDS             complement(25744..27543)
                     /gene="lepA"
                     /locus_tag="JN701_000884"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY7363409.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27741..28220)
                     /gene="rseC"
                     /locus_tag="JN701_000885"
     CDS             complement(27741..28220)
                     /gene="rseC"
                     /locus_tag="JN701_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY7363410.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28217..29173)
                     /gene="rseB"
                     /locus_tag="JN701_000886"
     CDS             complement(28217..29173)
                     /gene="rseB"
                     /locus_tag="JN701_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY7363411.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29173..29823)
                     /gene="rseA"
                     /locus_tag="JN701_000887"
     CDS             complement(29173..29823)
                     /gene="rseA"
                     /locus_tag="JN701_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY7363412.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29856..30431)
                     /gene="rpoE"
                     /locus_tag="JN701_000888"
     CDS             complement(29856..30431)
                     /gene="rpoE"
                     /locus_tag="JN701_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY7363413.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30428..30583)
                     /gene="rseD"
                     /locus_tag="JN701_000889"
     CDS             complement(30428..30583)
                     /gene="rseD"
                     /locus_tag="JN701_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY7363414.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30839..>31371
                     /locus_tag="JN701_000890"
     CDS             30839..>31371
                     /locus_tag="JN701_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7363415.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRR"
BASE COUNT         7502 a         8406 c         7917 g         7546 t
ORIGIN      
        1 acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacagt ggtgaataat
       61 gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ctcatattgc
      121 gcaacctccc cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc
      181 tgctgttgta aatcgccata cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc
      241 gcctcaatac tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc
      301 tgtgcggttt gctgttcacc cagcagtcgc agcgccattc cttgccagaa gagataatca
      361 accggctgat cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga
      421 cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag
      481 aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa
      541 tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc
      601 caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc
      661 cacgggtgga atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga
      721 tgccacaaat tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta
      781 ttttccagac gcgccagtcg tttctccggt gtggctccac ttaacttatc aagcaaatcc
      841 cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc
      901 gcggccagct cataatcgtg ttgcttattc cacgcatgga tcgctaaccc gcgccagccg
      961 tcggcaaact ccggcgacat ctctacgcaa cgttgccaaa aggcaatggc tttgttgtag
     1021 ctacgtttgt tgtagtagaa gcaggccagt aaatggcgag caaaccagca ctcttcaata
     1081 ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca
     1141 tcaatggctt ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg
     1201 taaagcggca gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc cagggtgggc
     1261 attccccagt taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacac
     1321 agaccgcgcc actgcgcgag agctgattcg ctacgaccat cgaaccagtt cagccaccac
     1381 agagtggcgt tcagcggata atcgcgcagc agtttctcgc gctgcacacg cgcgttgtcc
     1441 tgacgaccac ttaacaccag cagcagatta tgcaggcaaa gcacttcctg attggttggg
     1501 caggcgcgaa gactttgttg gcaaaaatcc agaccagcgt cgaagttacc attacgcgcc
     1561 gccagtcgtg ccaagccata atagccaccg gctttgctgt tgccgctcca gaccgcacgc
     1621 cagaaatcct cttcggcttg ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc
     1681 aaactcgcct gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagagcctga
     1741 gtggcatacg ccaccgcttg cgggaaatcg gcgcggttat attccagcat cgccagcgcc
     1801 aggttacagc ggtaatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt
     1861 gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta
     1921 ctggtaatgt cttgtgctgc cagtggcgct ttggcgacgt ccagcaacgg caactcttgc
     1981 ggctgatgtt cctgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc
     2041 agcctttcag gattgatacc gtgcaacacg ccctggatgg cggtcgcagg catcagtgcc
     2101 acggcatcat caagtaacgc gttgcatttg ccgatttcgc ggatcgccag gcggtatccg
     2161 ttcaacggag agatggcata cagcccccac tcaatccccc gcttactacg ctggagtttt
     2221 atcaccgcat cgcgggaggc attttgcacc atgcccaaag aatgataagg caggaaatac
     2281 tgctcaaaac gcttctcttc gtaagcatca agccaggtaa aatcgggctg gttatcggca
     2341 aaaataccgg tcatcagttc gatatacggg ccgttattgt cggtcagact cttatcccac
     2401 gcctggccaa attcactgtg tccccaactc cactgttttt tacctggcgc aatatggtgg
     2461 ttggcaacgt gcagcaaacc gccatcttca tcgtgacacc acgcgccgac aaaatcgtac
     2521 tgcgattttt cggccatata tgaggttgta acaggcacat ttttatagca agaaatgtcc
     2581 actccggcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg
     2641 gcccgtttgc cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct
     2701 tccccccctt tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttaccg
     2761 ttatagacgc ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc
     2821 atcacctgta aaccatgcat cggctccgtt tcgcctaccc acaccgtctg tgcaccgtct
     2881 tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg atgttgcggc
     2941 cagttaaact caatcccacc ggaaatccac ggccccagca gccccaccag cgcaggttta
     3001 atgacttcat tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc
     3061 cgaccgccca gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc
     3121 tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc
     3181 gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc
     3241 ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg
     3301 ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct
     3361 ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag
     3421 cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt
     3481 accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg
     3541 cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat attgacgatg
     3601 aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga
     3661 cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa
     3721 gtccgaaagg tgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt
     3781 ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc
     3841 tggcgctggc aatccacggt caggttgatc cggtgaccgg cgtgtcgcaa accatgccgc
     3901 aagcgccgtg gacaacgccg gttgaggtga agtatctgct ggaagagaag ctgggcattc
     3961 gggtgatggt cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt
     4021 cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga
     4081 ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acaaattggt cacaccatcg
     4141 ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct
     4201 cgttaagtgc attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc
     4261 agcttgatcc tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag agtggagaac
     4321 cgtggatcac cagctgggtt gatcgctctg ccaatgccat tggtttgagt ctgtataact
     4381 tcctcaacat cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg
     4441 agaactggct taatactatt attcgccaga caggatttaa cccgttcgac cgcgacgaag
     4501 gaccgagcgt gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc
     4561 tgggaattgg ctatttgtat gttgaggcgc agttacgaca gatttgatgg cgcgataacg
     4621 tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa
     4681 accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca
     4741 tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct
     4801 ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg
     4861 ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg
     4921 gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta
     4981 tcagtgccgc cggaaaccac tttgtagccg cgctcgagga acacttctac catcgcttta
     5041 gcgtttttcg cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga
     5101 gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg
     5161 gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg
     5221 cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg
     5281 ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa
     5341 gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc ggaatatgca
     5401 gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg
     5461 tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag
     5521 aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg
     5581 gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc
     5641 ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt
     5701 tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca
     5761 tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg
     5821 gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac
     5881 agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc
     5941 agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat
     6001 aaccgaatca ttgtgcgata acaggtcttg acaaaggaat tcacgcaaac gattaccttc
     6061 aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg
     6121 tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt
     6181 acattgcagg gctatttttt ataagatgca tttgaaatac atcaattaag atgcaaaaaa
     6241 aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac
     6301 tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc
     6361 cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc
     6421 tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag
     6481 cggtagaaaa aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca
     6541 tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc
     6601 tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg
     6661 aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca
     6721 ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg
     6781 gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag
     6841 gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct
     6901 atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg
     6961 ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg
     7021 cagatgacac accagtgacg ttaatctctt ccggtgttgg tcaaacgcca atgctggcaa
     7081 tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag
     7141 aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc
     7201 gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt
     7261 ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa
     7321 tgcagttcta tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg
     7381 atctgggcgt gaagcaggaa aacatttatt acgaatgctt tggcccgcat aaggtgctgt
     7441 aatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt
     7501 cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac
     7561 cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa
     7621 tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg
     7681 cgccgcggta cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg
     7741 tcatgccggt aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt
     7801 ttataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct
     7861 gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc
     7921 gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga
     7981 cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg
     8041 cctcaacaaa ggttggcagc gccgtgtttt caccctccag cgcctgctct actaatgcat
     8101 cgctaataac cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc
     8161 gcacattacc cggccagcta gcggtcatca ggcgtttcat cgcatcagtg gagaacgcgc
     8221 ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agaagcggaa
     8281 tgtcttctgt gcgctctgcc agagccggaa ttttcaggct gacaacgttg aggcggtaat
     8341 agagatcttc gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg tgagtggcag
     8401 aaataatccg cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct
     8461 cctgcaacac gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga
     8521 gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag
     8581 taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc
     8641 cacagttaat agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg
     8701 cgaaaatctc tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg
     8761 ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct
     8821 cgcgccagcg ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat
     8881 atagcgcgtc tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag
     8941 caacggcatc ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca
     9001 ctttctggat ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga
     9061 taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg
     9121 tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat
     9181 cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg
     9241 gatggcgcgg gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg
     9301 cgggtcgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc
     9361 agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac
     9421 tgctggagtt tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg
     9481 tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca
     9541 atacgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc
     9601 ccttgtttaa aggtattttg ccagcagccg tcgtcatatt ggcgagcttg ctgacgcgac
     9661 tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt
     9721 tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc
     9781 agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc
     9841 tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat
     9901 aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat
     9961 tgttgcagac agtctggaca tggacagcgc ggagaaaccg gagcgtacat atcgtacgtg
    10021 aagatttcga gcactgcccg gagccgaact gacaaataaa atagcctgat aggataggct
    10081 gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc
    10141 cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc
    10201 ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg
    10261 gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa
    10321 tataaacccg cgcaccatgt gaactgctgc gaaggcaaat gttaccggat tcagccccgt
    10381 agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca
    10441 ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc
    10501 tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg
    10561 cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc
    10621 ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa
    10681 cctggtcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat
    10741 gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta
    10801 accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat
    10861 tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc
    10921 cgataatcat ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg
    10981 attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag
    11041 agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa
    11101 gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt
    11161 tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat
    11221 cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt
    11281 ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt
    11341 cactacgccg ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg
    11401 cattcaggct ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa
    11461 atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt
    11521 tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag
    11581 ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga
    11641 tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat
    11701 tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct
    11761 tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg
    11821 ttgctttgag ggatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct
    11881 aaaaagagac agcttaagat aaacttatta gattacaata aaatcaatca cttaagtgtc
    11941 gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc
    12001 ggagttggga gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa
    12061 tgaactgatg aattacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc
    12121 atcctcgccc cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg
    12181 gtgcggcatc ataatggtga ctcgaccact ttcagtcgtg actgccgtaa taccgttcgg
    12241 tgaaccgttc gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg
    12301 cagtgccacc agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac
    12361 gcgcccttca ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacaa
    12421 cagagacggg ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt
    12481 attgcgcaca aaacgtggcc acaactcact acctgggatc agttcgcgca ggttagacat
    12541 catctggcaa ccgttacata cccccagcgc cagcgtttgc ggacggtgga agaaggttgc
    12601 aaactcatcg cgtacacggt cattgaacag gattgacttc gcccaacctt caccggcacc
    12661 cagcacatca ccgtaggaga aaccaccgca cgcgaccagg gcgtggaaat cttccaggcc
    12721 cgtgcgtccg gtcagcaggt cactcatatg cacgtcgata gcatcaaagc ctgcacggtg
    12781 gaaagctgcc gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt
    12841 cggacgtgcg ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag
    12901 ttttacattc aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca
    12961 ctccgggttg tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa
    13021 cgtggtgcgg ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc
    13081 ggaaaccgcc tgccctacat aatggacaca atcagcaagc ccatgctgtg ccagtacgga
    13141 ctcgaccgct tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa
    13201 caacgccgcc aggcgatcgt cgcccagaga ggcaatatcc gcgttaatgc cgcaatgacc
    13261 agcaaaggcc atttccgcca gcgttaccag caggccgcca tcagagcggt cgtggtatgc
    13321 gagcagctta cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttgcgc
    13381 gacgtcgcgt acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt
    13441 tgcgcccagc gcgttattgc ctttgcccaa atcaatcagc aatagtgcgt tatcttcggt
    13501 agaaagctgt ggcgtgatgg tgtgacgcac atcttccacg cgggcaaatg cagaaatcac
    13561 cagcgacagc ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat
    13621 cgacatagag tctttaccca ccgggatcgt caggcccagc gccggacaaa gctcttcgcc
    13681 cacggcttta acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat
    13741 ccagttggcg gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt
    13801 taacgcttca ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc
    13861 acgctcgcca atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc
    13921 gcagttagcg accggcacct gccacggacc caccatctga tcgcgcgcta ccatgccagt
    13981 tacgctgcgg tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag
    14041 tatgcgtttc accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc
    14101 tttcagcgtt tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg
    14161 cagatcgatc ggctgattat caaaatgacg atcgtgtaga gaaagatgca gttcttcggt
    14221 cgcttcacca atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg
    14281 taattgatcg gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc
    14341 cagcgggctc atgcctggct cgtcgctcag aatgtcgcgc agttcaaatt taccgccacg
    14401 cccgccgtcg ctcaccagtt ccggcatagc gttagaaaga ccgcctgcgc caacgtcgtg
    14461 gataaacagt attgggttgg catcaccaag ctgccagcaa cggtcaatca cttcctggca
    14521 gcgacgctcc atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga
    14581 ctgaccagac gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc
    14641 gccgagaacg accagcttcg cgccaacgtt gatctcgcct ttttgtacgt gatcggcgcg
    14701 aatgttgccg atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc
    14761 gttgtggctg ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc
    14821 aaattcgttg ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt
    14881 gacaatgcgc tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caagaattcg
    14941 caggttggaa acggagaaac caaccaaacc cgctttcggc ttcgcgccgc gcccggtggc
    15001 accttcatcg cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc
    15061 cgtcgggtgg ttgtgagttt cgactttcat cagaatatgc gccggttcct gatggaaatc
    15121 atagcggccc gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc
    15181 ggcgttatct ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat
    15241 catcctgaac agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat
    15301 tttgtggcgg cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg
    15361 gttacgacca agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc
    15421 caggccaaga cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac
    15481 gcttgtgacc ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa
    15541 aaagacagtt tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt
    15601 ggtcagcgta ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg
    15661 tagcccgcag ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg
    15721 acgcggggtc accagcagaa gtttgccttg cggggcgtgg ctggcgagtg ccggaccata
    15781 tttcagcagg cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc
    15841 aaaatggaca tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc
    15901 cagcagtttg ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat
    15961 aagtctctcg tcttctaagc tttcggtgta cccaaggggg ggggaaacgg gcgtcattat
    16021 aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta
    16081 gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg
    16141 ccgctcattt atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac
    16201 gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct
    16261 ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa
    16321 ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct
    16381 gacttataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt
    16441 tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt
    16501 tgacgacctt gataatggta acgccgacct gctggcggca ggactggtct ataacagtga
    16561 gcgggtaaaa aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta
    16621 taaagtgggt cagtatcgcc cacgtacgct gtgcaacctg acggcggagc aactcaccgt
    16681 tgcaccgggt catgtggtgg ttaacgatct ccagaccctg aaagaaacaa aattcccgga
    16741 attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga
    16801 aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca
    16861 cccggagctc gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc
    16921 gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga
    16981 agacggtacg ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta
    17041 cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct
    17101 gtttgagaaa tacgccgaag aaattgacta gcgtttgctg gccgctattg cttatcagga
    17161 atcgcactgg gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac
    17221 caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag
    17281 cggtggcgtg cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa
    17341 cgagcggatc tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc
    17401 ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg
    17461 tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg
    17521 acatgaagcc tacgcttatg tcgaaaatat tcgtaagtat caaattagcc tggtgggtta
    17581 tctgcaagag aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc
    17641 ggcggtatcg cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca
    17701 gtcgtcatca aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga
    17761 agagaaacaa aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg
    17821 gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt
    17881 aatttccact cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc
    17941 gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc
    18001 tccggcacac attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata
    18061 attttgcatc accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc
    18121 atggcgacca atcgggcggt tccaaccttc gccgattacc cgattgttat gcactaatac
    18181 cgcgccgacc ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg
    18241 catccagtat tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc
    18301 gcggcgcatt atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg
    18361 cggggttact cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc
    18421 gctatagcca ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt
    18481 ttcatgtccc agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct
    18541 ggcgataaga ttaacccgcg gcagccaggg cttatcgaaa taaaccgctt caaccagcgg
    18601 ctgcggagag ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa
    18661 tcgctcctga accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc
    18721 ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact
    18781 catcggccag cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa
    18841 cggcaggaca agtaacgcat tcagcggttg gcgacgtagt aaatagcgca gaaaactgcc
    18901 gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg
    18961 agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag
    19021 cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat
    19081 ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtg tgtctcatat
    19141 tgcctgttgt cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg
    19201 tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca
    19261 acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca
    19321 gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt
    19381 ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca
    19441 aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc
    19501 cgctatgtac gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat
    19561 gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca
    19621 aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca
    19681 tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta
    19741 caccggtgta cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa
    19801 agtgctggcg attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct
    19861 gtataccatt gccgaagaac aagcgacaaa cagtgcttca atctctgctt gtcacgctca
    19921 gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc
    19981 agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg
    20041 tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta
    20101 aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg
    20161 gagatcatat ctacgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga
    20221 caccaacctg ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat ccggcgcatg
    20281 tcgagacaga agaacagttg tgggataaat ttgtgctgat gcaccacgcg gataaaattt
    20341 aatattctac tctggaagta gagtattaat tatattactg ggaagcctta acgccattat
    20401 atttatttaa ttgatgacat tagcataatc attcactaag ttaatttata tagtatctgc
    20461 caagacactt atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa
    20521 gtacgtttcc ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt
    20581 gatgacccgt ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg
    20641 cagccgttca gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg
    20701 catgctgcac tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt
    20761 ggcatggtgt aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga
    20821 attggcggtc acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt
    20881 atgctaaaga ttgcaagttg cttgtaaaag ataagtacac tgatccataa tcgctgttgt
    20941 tgagggtgca tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt
    21001 cgggggtttt actttttaaa gagaacggta ttattttaac tttcaataat taccgtggca
    21061 caagcatagt ggcgttcatc tgccagcgtt acatgcatat ttacaacgcc gagcttttcc
    21121 gccagtttta atgcctcacc ccatagccgt agccgtggtt tgccgagctc atcattgaat
    21181 acttcaaatt gattaaacgc cagaccattg cggatcccgg tgccaaacgc ttttgctgcg
    21241 gcttctttca cagcaaaacg cttcgccaga aaacgcaccg gctggtggtg cgttttccag
    21301 attgcccatt cgttatcgct taatacgcgg cgtgccaggc gctcaccgga tcgggcgatc
    21361 actgcttcta tgcgagcgat ctctacgata tccgtgccta aacctaatat tgccattagc
    21421 cacgcgcttc cagcatcaga cgcttcattt ctgccaccgc atctttcagt ccggtcatca
    21481 ctgcacggcc aataatggca tgaccgatat tcagttcatg catctcaggg atggcggcaa
    21541 tggctttcac gttgtgatag gtcagaccgt gtccggcgtt aactttcaga ccgagactag
    21601 cggcaaaggt cgcggctttg gcgatacgca ccagctcttg cgcctgttcg gcgtcagttt
    21661 tggcatcagc atagcaaccg gtgtggatct cgataaacgg tgcgccaacc tctgccgcag
    21721 ctttgatctg ctcttcatcg gcgtcgataa agagagaaac ctgaatcccg gcatctgtca
    21781 gacgtttgca ggcatcgcgc attttttcac gctgccctgc aacgtccagg ccgccttcgg
    21841 ttgttacttc ctgacgcttt tccggtacca ggcagcaaaa atgtggcttc gtctcaacgg
    21901 cgatcgccag catctcttcg gtcactgcca tctccagatt cattcgggta tccagcgtct
    21961 gccgcaggat gcgcacatcg cggtcagtaa tgtggcggcg atcttcacgt aaatgcacgg
    22021 taatgccgtc cgctcccgcc tgctcggcaa taaacgccgc ctgcaccgga tccgggtaag
    22081 cagtaccgcg tgcgttacgc agcgttgcaa tatggtcaat gttgacgcct aacagtaatt
    22141 cagccatgac aatcctcatc attcataatg tgttttcacc gttcgcttag gcataaactg
    22201 ccggaacagt tccctgctct ttaatggttt accgccaaga tacggcttaa gcgccatgcg
    22261 ggtaaagcgt ttcgcggcgc gtagtgtgtc tgcgtcagga aattcccgcg cgtttaacgc
    22321 ttttaactgc cttccggtga acgttttatt gtcgataacg acgcttgcga taaacccttt
    22381 ttcttcgcga taacgatacg tcatggtgtc atctaccggc tcgccgctac ccgcgcaata
    22441 ggtaaaattg acgccatagc ccagatgccc gagcagtgcc agttcaaagc ggcgcagcgc
    22501 gggttctggc gtaccagtgg cacctgcaag agactgaatg cagtgcaaat aatcgaaaaa
    22561 gagttcagag aagcgcgtct cgtattccag tacgcgggag agaagttcgt tgatgtacag
    22621 accgctgtaa agcgtgatac cgcttaatgg cagcgccagc gagacagctt cagcactgcg
    22681 cagcgttttg acttcgccac gcccgccgaa acgtagcaag agaggggtga aaggctgtaa
    22741 tgcacctttc agagtagagc gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc
    22801 cgattcctcc gtgaagacgt ccagcatcag gctggtttcg ctccacgggc gactatgcag
    22861 gacaaatgcg cgctgccagc cttccatcgg agtgactctt aaagatcgtc aacgtaaccg
    22921 agactgcgca gtgcgcgttc gtcgtcggcc caaccggatt tcacttttac ccacagttca
    22981 aggtggacag gcgcttcgaa catttcctgc atgtctttac gcgcttcaat cccgatggtt
    23041 ttgatcttgg cccctttgtt gccaataacc atcttcttct gcccttcacg ctcaacgaga
    23101 atcaaaccat tgatgtcata accaccgcgt tcgttagaga cgaaacgttc gatctccacg
    23161 gtcacggagt acggcagttc agcgccgagg aaacgcatca gtttttcgcg gatgatttca
    23221 gacgccataa aacgctgtga gcgatcggtg atgtaatctt ccgggaagtg atgggtcgct
    23281 tcaggcagat gcttacgcac gattgctgcg atagtgtcga cattcagccc ggtttcagca
    23341 gagatcggca cgatatcgag gaagttcatc tgacttgcca ggaactgtaa gtgcggcagc
    23401 agatcggctt tttcctgcac gttatccact ttgttcaccg cgaggatcac cggcgctttg
    23461 ccatcgcgca gtttgttgag caccatttcg tcgtccggcg tccagcgggt gccttcaacg
    23521 acaaaaatca ccagctcaac gtcgccaata gagctgctcg ccgctttgtt catcaggcgg
    23581 ttaatagcgc gtttttcttc catatgcagg ccaggggtat caacgtagat cgcctgatac
    23641 gcgccttcag tatggatccc cacaatgcgg tgacgagttg tttgcgcttt gcgggaggtg
    23701 atggagatct tctgccccag cagtttgttc aacaatgtgg atttgccaac gttcggacgt
    23761 ccgacgatgg caataaatcc gcagtaactt ttatcgatgc tcattccagc tccagttttt
    23821 tcaacgcctg ttcggcggca gcctgctcag ccttacgacg gcttgaacct gtgccaacca
    23881 ccggttcact caggccgctg acctggcagt ggatagtaaa ttcctgatcg tgcgcttcgc
    23941 cacgtacctg gactaccaga taagtcggca gcggcagatg gcgaccctgc aaatattctt
    24001 gcaagcgcgt tttcggatct ttttgtttat cgcctgggct aatttcgtcc aaacgagttt
    24061 gataccagtt gaggattaat ttctcgacgg tttgaatatc actgtcgagg aatacgccac
    24121 caattaatgc ttcgacggta tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa
    24181 gttcaccagg ccctaaacgt aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca
    24241 gcgtattgcc acggaccagc gtggcgcgca tccggctcat atcgccttca tccacacgag
    24301 ggaaacggtg ataaagcgca ttggcgataa cgtagctcag aatagagtcg cctaaaaatt
    24361 ctaaacgctc gttatgtttg ctgctggcac tacgatgagt taatgcctgc tgcaacagtt
    24421 cctgatgatt aaaagtgtag cccagcttcc gttgaagccg attaattacg atggggttca
    24481 tgcgatacca ataaataaat gcgtcaacaa ttcagcacac gaaacagacc tgatatacat
    24541 ggctctgcta actgcttcgc tgcagtttca gtttaccggt atatggggac caacgctgtt
    24601 tcgtgtgccg tggcaacctg gaggtgccaa ccttaaactt cgggggaata ttctatacac
    24661 aacgacgggg gatgtcgtta gccacgggag atttatctca taaataattc acgttgtcgc
    24721 cataacggcg acaacgtgaa cgaagatggg ctattaatgg atgccgccaa tgcgacttaa
    24781 gcgcacacct gtcggccatt cgccttcttg cttatcgaaa ctcatccaga tagccgttgc
    24841 ccgaccgacc agattcgctt caggcacaaa accccagtaa cggctgtccg cgctgttgtc
    24901 gcggttgtcg cccatcatga agtattgtcc cggcggaaca atccaggttg ccagttgttg
    24961 ccctggctgc tggtaataca tccccacctg gtcctgcgca atcggcactg tcagaattcg
    25021 gtgcgtcaca tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt
    25081 ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa
    25141 ggtctgaacg aaatcgctcg gttccacgtt ggagtaggtg accggtagcg cgttttcaca
    25201 cgcctggccg gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcgtaagt
    25261 gactttatcg cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg
    25321 atatttaaag accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg
    25381 gtagatagga tctttcatgc cataagcaaa cttctctacc agaataaaat caccgattaa
    25441 cagagtcggc atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgtac
    25501 aatcaatacg atagccagca ccggaaaaac agaagcaccg gtttccagcc agccaggctt
    25561 cggcgcaacc tttttcaggg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc
    25621 tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc
    25681 cgtcaccagt gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa
    25741 gggttatttg ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc
    25801 gacgttaccg atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt
    25861 cttacggctg atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac
    25921 ggtggatcgc gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg
    25981 gcgtgggatc agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc
    26041 acggtgggtg atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg
    26101 taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga
    26161 ggtagatttc aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt
    26221 cagcgccacc tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca
    26281 cagcgtaata acgttgccga gatatgcctg cggcagcagc atgtgacact ctgcaatcgg
    26341 ctcgcgcagt tcgtaaatgt tatttaccgc aggcagcttg gatgggctgt cgacgtagat
    26401 aacttccctt gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc
    26461 cagatcgtat tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccaaggaa
    26521 gccgcagcgg aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaggc
    26581 atcattcagg ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac
    26641 cgggaacaga ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc
    26701 tgccggatta cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat
    26761 cgcacatacc agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg
    26821 cgtgaagatg cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt
    26881 gtcgccctta cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt
    26941 gtcgaaccat gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg
    27001 cggaatgtcg cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga
    27061 acagcgcacc gcgtcagtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg
    27121 ttcaggatcg gctgccggca ggtcaatctt gttcagtact ggcaccactt cgagatccat
    27181 ttccatggcg gtgtagcagt ttgccagggt ttgcgcttcc acgccctgcc cggcgtcgac
    27241 caccagcaat gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtctac
    27301 gtggcccggg gtgtcgataa agttaagctg ataggtttcg ccgtcagacg ctttgtagtc
    27361 cagcgtcacg ctttgcgctt tgatggtaat gccacgctca cgctcaagat ccatggaatc
    27421 gagaacctgc gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc
    27481 agacagcgtc gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt
    27541 cataaagtat gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt
    27601 ctgagcttaa cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag
    27661 gaaatgttca taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa
    27721 atgcaacaaa gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca
    27781 aggccaggcg gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac
    27841 ttacgcgagt agccgcgcgc aatcaggaac ccgccaatac cgccgaggac cgcaccacat
    27901 aatgctgcga cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc
    27961 accagcggcg acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc
    28021 aattccactt tttgccccgg cactaacggt tcatcacagg gtacgacaat ggtatgcgtg
    28081 gtttgcgggc caagtttatt caacacgcgg ctaccgcaac cggcgcgtga agcgcagctg
    28141 ctgcatgaag ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg
    28201 gtagcccact ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg
    28261 ccgtttgcgg cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac
    28321 tgacggttct gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa
    28381 cgtttaccga gaagctgaat aatccgtcag aatagagacg tgattcgata ggcatgttgt
    28441 ccatcgtcgg tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg
    28501 ttggcgtcca gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat
    28561 ttgccttcgc cagcgtctgc atactgctgc tgatatcctg attgacgtta aaagcaatca
    28621 cgcgaaattg ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt
    28681 tcgattcggt gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc
    28741 gaatgacttc gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt
    28801 aaggagaaag gcgtttgaaa tcggtataga tgagcgatgg cagagaatca acaatgtaat
    28861 cgccattaag cgtgaacggt tcaagtcccg gttcaaaata gctgatttca tcgccgcgct
    28921 gtaccacttc ccggcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga
    28981 ggcgtgcatg tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca
    29041 gctcgtaatt cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg
    29101 gagtggccga ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc
    29161 aaagttgctt cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc
    29221 ggcttgctgg gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag
    29281 ttcgtaatcc tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg
    29341 accattgttt gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat
    29401 catcggcagt gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt
    29461 atagtgctgg acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt
    29521 aagctgtgcc gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg
    29581 cgcaggctgg gcttccggga tcaatgtcgc cggttgacgt actggctctt cttcaatggc
    29641 ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga
    29701 gtcacggatt aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc
    29761 gttaagcagc tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg
    29821 catgcctaat acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact
    29881 ttgttatcaa tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa
    29941 tccatgatag cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc
    30001 atgcgcaaat cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct
    30061 gacaacatta agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt
    30121 tcagcttcaa tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa
    30181 tttttcgctg tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg
    30241 aacgaatcca gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca
    30301 cccgacggca catagcggga aaccagactc gccactttat gctgatagcg cactaccagt
    30361 aagttaaagg ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac
    30421 tgctcgctca tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc
    30481 actccattag ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc
    30541 cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt
    30601 ctacagaatc tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt
    30661 aacccaacgg cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt
    30721 ctcgccgccc tgcatcagcg tgtttagcaa ctgtaacaaa tattaaaata gcaggtgttt
    30781 attcgcacaa catgatgcta tgctgaccaa accgtgttta gtaaattaaa caaagaaaat
    30841 gaatactctc cctgaacatt catgtgacgt gttgattatt ggtagcggcg cagccggact
    30901 ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt
    30961 aacggaaggt tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga
    31021 cagcattgac tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgcca
    31081 tgcagttgaa tttgtcgcca gcaatgctcg ttcctgtgtg caatggttaa ttgaccaggg
    31141 ggttttgttt gatacccacg ttcaaccgaa tggcgaagaa agctaccatc tgacccgtga
    31201 aggaggacat agtcaccgtc gtattcttca tgccgccgac gccaccggta gagaagtaca
    31261 atccacgctg gtgagcaagg cgcagaacca tccgaatatt cgtgtgctgg agcgcagcaa
    31321 cgcagttgat ctgattgtct ctgacaaaat tggcctgccg ggcacgcgac g
//
LOCUS       DADLTD010000024        30908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLTD010000024 DADLTD010000000
VERSION     DADLTD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.024"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            248..631
                     /gene="secE"
                     /locus_tag="JN701_000891"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="JN701_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY7363416.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="JN701_000892"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="JN701_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY7363417.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="JN701_000893"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="JN701_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY7363418.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="JN701_000894"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="JN701_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY7363419.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="JN701_000895"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="JN701_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY7363420.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="JN701_000896"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="JN701_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY7363421.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="JN701_000897"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="JN701_000897"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY7363422.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="JN701_000898"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="JN701_000898"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY7363423.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="JN701_000899"
     CDS             12677..13216
                     /locus_tag="JN701_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HAY7363424.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="JN701_000900"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="JN701_000900"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY7363425.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15601)
                     /gene="thiG"
                     /locus_tag="JN701_000901"
     CDS             complement(14756..15601)
                     /gene="thiG"
                     /locus_tag="JN701_000901"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY7363426.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAEMLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSHFAHATSPLTGF
                     LEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="JN701_000902"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="JN701_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY7363427.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="JN701_000903"
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="JN701_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY7363428.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLSRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="JN701_000904"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="JN701_000904"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7363429.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="JN701_000905"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="JN701_000905"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY7363430.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="JN701_000906"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="JN701_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY7363431.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="JN701_000907"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="JN701_000907"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY7363432.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="JN701_000908"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="JN701_000908"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY7363433.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="JN701_000909"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="JN701_000909"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY7363434.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="JN701_000910"
     CDS             22396..22986
                     /locus_tag="JN701_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY7363435.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="JN701_000911"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="JN701_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY7363436.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="JN701_000912"
     CDS             23458..24153
                     /locus_tag="JN701_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7363437.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="JN701_000913"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="JN701_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY7363438.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="JN701_000914"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="JN701_000914"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY7363439.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="JN701_000915"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="JN701_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="JN701_000916"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="JN701_000916"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY7363440.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="JN701_000917"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="JN701_000917"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY7363441.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7324 a         7980 c         8425 g         7179 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cctggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggtg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga atatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttaccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 tgacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgcg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gcgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggctgcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacctggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc ttgaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgcacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa agctcgtcgc aactgctctt taacaattta tcagacaatc
    30901 tgtgtggg
//
LOCUS       DADLTD010000025        30552 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLTD010000025 DADLTD010000000
VERSION     DADLTD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.025"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1370
                     /locus_tag="JN701_000918"
     CDS             <1..1370
                     /locus_tag="JN701_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7363442.1"
                     /translation="TLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSW
                     LAGAPLALLRRGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSG
                     LRAVAVMFMNPLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLV
                     VTALAGVALVVWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALW
                     GWFTDLLTPVKSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGI
                     VSKEAAKAKLPESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFG
                     IVGENGPEIVNGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAM
                     VSRAATVPPVQRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQ
                     GGYDA"
     gene            1367..1852
                     /locus_tag="JN701_000919"
     CDS             1367..1852
                     /locus_tag="JN701_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7363443.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            1849..2949
                     /locus_tag="JN701_000920"
     CDS             1849..2949
                     /locus_tag="JN701_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAY7363444.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            3040..3258
                     /locus_tag="JN701_000921"
     CDS             3040..3258
                     /locus_tag="JN701_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="in E. coli K-12 this is in prophage P2 remnant;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7363445.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(3494..5179)
                     /locus_tag="JN701_000922"
     CDS             complement(3494..5179)
                     /locus_tag="JN701_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY7363446.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALNMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            5449..5826
                     /locus_tag="JN701_000923"
     CDS             5449..5826
                     /locus_tag="JN701_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363447.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(5856..6113)
                     /gene="grxA"
                     /locus_tag="JN701_000924"
     CDS             complement(5856..6113)
                     /gene="grxA"
                     /locus_tag="JN701_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY7363448.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            6273..6560
                     /locus_tag="JN701_000925"
     CDS             6273..6560
                     /locus_tag="JN701_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY7363449.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            6544..7266
                     /gene="nfsA"
                     /locus_tag="JN701_000926"
     CDS             6544..7266
                     /gene="nfsA"
                     /locus_tag="JN701_000926"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY7363450.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            7327..8229
                     /gene="rimK"
                     /locus_tag="JN701_000927"
     CDS             7327..8229
                     /gene="rimK"
                     /locus_tag="JN701_000927"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY7363451.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            8317..8793
                     /locus_tag="JN701_000928"
     CDS             8317..8793
                     /locus_tag="JN701_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY7363452.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            9144..10256
                     /gene="potF"
                     /locus_tag="JN701_000929"
     CDS             9144..10256
                     /gene="potF"
                     /locus_tag="JN701_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY7363453.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGQATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENRG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            10351..11484
                     /gene="potG"
                     /locus_tag="JN701_000930"
     CDS             10351..11484
                     /gene="potG"
                     /locus_tag="JN701_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY7363454.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            11494..12447
                     /gene="potH"
                     /locus_tag="JN701_000931"
     CDS             11494..12447
                     /gene="potH"
                     /locus_tag="JN701_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY7363455.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            12444..13289
                     /gene="potI"
                     /locus_tag="JN701_000932"
     CDS             12444..13289
                     /gene="potI"
                     /locus_tag="JN701_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY7363456.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            13349..13837
                     /locus_tag="JN701_000933"
     CDS             13349..13837
                     /locus_tag="JN701_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY7363457.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            13878..15005
                     /gene="rlmC"
                     /locus_tag="JN701_000934"
     CDS             13878..15005
                     /gene="rlmC"
                     /locus_tag="JN701_000934"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY7363458.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(15180..15911)
                     /gene="artJ"
                     /locus_tag="JN701_000935"
     CDS             complement(15180..15911)
                     /gene="artJ"
                     /locus_tag="JN701_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7363459.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(16202..16870)
                     /gene="artM"
                     /locus_tag="JN701_000936"
     CDS             complement(16202..16870)
                     /gene="artM"
                     /locus_tag="JN701_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY7363460.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(16870..17586)
                     /gene="artQ"
                     /locus_tag="JN701_000937"
     CDS             complement(16870..17586)
                     /gene="artQ"
                     /locus_tag="JN701_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY7363461.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(17593..18324)
                     /gene="artJ"
                     /locus_tag="JN701_000938"
     CDS             complement(17593..18324)
                     /gene="artJ"
                     /locus_tag="JN701_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7363462.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(18342..19070)
                     /gene="artP"
                     /locus_tag="JN701_000939"
     CDS             complement(18342..19070)
                     /gene="artP"
                     /locus_tag="JN701_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY7363463.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(19288..19803)
                     /locus_tag="JN701_000940"
     CDS             complement(19288..19803)
                     /locus_tag="JN701_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7363464.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            19929..20252
                     /locus_tag="JN701_000941"
     CDS             19929..20252
                     /locus_tag="JN701_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY7363465.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            20249..21079
                     /locus_tag="JN701_000942"
     CDS             20249..21079
                     /locus_tag="JN701_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY7363466.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(21076..22086)
                     /locus_tag="JN701_000943"
     CDS             complement(21076..22086)
                     /locus_tag="JN701_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7363467.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(22185..23615)
                     /locus_tag="JN701_000944"
     CDS             complement(22185..23615)
                     /locus_tag="JN701_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7363468.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(23626..24627)
                     /gene="ltaE"
                     /locus_tag="JN701_000945"
     CDS             complement(23626..24627)
                     /gene="ltaE"
                     /locus_tag="JN701_000945"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY7363469.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(24664..26382)
                     /gene="poxB"
                     /locus_tag="JN701_000946"
     CDS             complement(24664..26382)
                     /gene="poxB"
                     /locus_tag="JN701_000946"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY7363470.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(26515..27483)
                     /gene="hcr"
                     /locus_tag="JN701_000947"
     CDS             complement(26515..27483)
                     /gene="hcr"
                     /locus_tag="JN701_000947"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY7363471.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(27495..29147)
                     /gene="hcp"
                     /locus_tag="JN701_000948"
                     /gene_synonym="priS"
     CDS             complement(27495..29147)
                     /gene="hcp"
                     /locus_tag="JN701_000948"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY7363472.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSTRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(29291..30190)
                     /gene="lysO"
                     /locus_tag="JN701_000949"
     CDS             complement(29291..30190)
                     /gene="lysO"
                     /locus_tag="JN701_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY7363473.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWHNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         7278 a         7773 c         8221 g         7280 t
ORIGIN      
        1 gcaccttagc ggtggcgctg gctgcagtgc tggggccgct ggcagtcatc cgtctgggat
       61 tctctgtgct gggtatcaaa acgttacctt ccgttacggc aacagtaacc cgaaccagca
      121 acgcgttgtc ctggctggct ggcgcaccac tggcactgct gcgacgcggg cttgcttcat
      181 cgggcaacgc tgcaggttta cttactgcgc cgttgtcgtc tttgcgccgc acggcatcac
      241 tgacgggaaa tgtcctgaaa actgtagcag gtgcgccggt tgcacttttg cggtctggat
      301 tatccggttt acgtgcggtt gctgtgatgt ttatgaatcc actggcggta ctacgcggcg
      361 gactggctgc cacaggcacg gtgctgcgta tgctcgcatc cggtccactg gcgatgctgc
      421 gcgttgccct gtatgccgtc tctggtctgt taggtgctct gctcagtctg ataggtcttg
      481 tggttactgc actggcgggt gtggcgctgg ttgtctggaa atactggcag cccatcaccg
      541 catttctcgg tggcgtggtg gaaggattca aagcggcggc aggtcctgtc agtgccgcgt
      601 tcgaaccgct taagcctgtg ttccagtgga ttggcgacaa agtacaggcg ctgtggggct
      661 ggtttactga tctgctgacg cccgttaagt cgacctctgc cgaactgcag agtgcagcgg
      721 caatggggcg acgattcggg gaggctctgg cggaagggct gaatatggtc atgcatccgc
      781 tggactccct gaaatccggc gtttcctggt tgctggagaa acttggcatt gtcagtaaag
      841 aggccgcaaa ggcgaaactg ccggaaagcg tgacgcgtca gcaacctgcg acggtgaatg
      901 cagacggtaa agtgatgatg ccatcgggtg gttttccgtc atggggatat ggctttgcgg
      961 ggatgtatga cagcggcggg tatatcccgc gcgggcagtt tggcatcgtc ggtgaaaacg
     1021 ggccggaaat tgttaacggc ccggcaaatg tgaccagccg gagaaataca gctgcactgg
     1081 ctgccgttgt tgccggaatg atgggtgttg ctgctacgcc agcagagctt ccaccgttgc
     1141 accctttggc acttcccgcg aaaggcggtg aagcgatggt gagtcgtgca gccactgtgc
     1201 cgcccgttca acggattgag gcaccgatgc agatcatcat tcaaacgcag ccaggacaaa
     1261 gtgcgcagga tattgcgcgg gaggtggccc gccagcttga tgaacgtgaa cgcaggctga
     1321 aggcaaaagc caggagtaac tacagcgatc aggggggata cgacgcatga tgatggtgct
     1381 gggattgtac gtgtttatgc tgcgcaccgt gccgtatcag gagctgcagt atcaacgcag
     1441 ctggcgacat gcggcaaaca gccgggtaaa ccgacgcccg tcaacgcagt ttctgggacc
     1501 ggacaacgac atgctgacgc tttctggtgt tcttatgccg gaggtaacgg gcggcaggct
     1561 gtcgttgctg gctctggagc agatggcaga acagggaaaa gcatggcccc tgattgaagg
     1621 cagcggcacg atttacggca tgtatgtgat tgagggactg aatcagacta aaacggagtt
     1681 tttccgcgac ggtatgccgc gccggattga gttcaccctg tcgctaaaac gcgtggatga
     1741 atccctgtcc gatatgttcg gtgatctcag tgcgcaactg aataatctgc agggaacggc
     1801 aacatctgcc ttaagcgata tcagtaaaac ggtcggaggg ctgctgtcgt gaatttcagc
     1861 tctgaactgc ttaacaaagg caacaaaact ccggcattca gcatcagtat tgaaggcagg
     1921 gatatcacca ctgtgctgga caaccgcctg atggggctga cgctgacgga taaccggggc
     1981 tttgaagcgg accagcttga tctggagctg gacgacgccg atggaaaaat cgtgctgccg
     2041 cgccgtgggg ctgtcattac gctggcgctg ggctggaagg gacagccgct tttcccgaaa
     2101 ggggcattca cggtggacga gattgaacac actggcgcac cggatcgcct gactatccgg
     2161 gcgcgaagtg ctgattttcg tgaaacgctg aatacccgcc gtgaaaagtc gtggcacaag
     2221 accaccgttg gggaagtggt gaaggaaata gctgcgcggc acaaactgaa gatggcattg
     2281 ggtgaagact tgtcggataa actcgtggag catatagacc agactaatga gagtgacggc
     2341 agttttttga tgcggctggc gcgccagtac ggtgctattg cgtcggtgaa aaatggcaat
     2401 ctgttattca tccggcaggg gcagggtaaa agcgccagcg gtaaaccact accggtgatc
     2461 actatcacac gcaaggacgg cgacagtcac cgctttaccc tggcagatcg cggagcttac
     2521 acgggcgtca ttgccagctg gttgcatacc cgcgaacccg cgaagaaaga aagcaccacg
     2581 gtgaagcgta agcgcaggac taagaagcag aagaaagagc cggaagcgaa gcagggcgat
     2641 tacctggtgg gtacggatga aaacgtgctg gtacttaatc gcacttatgc caaccggagc
     2701 aacgccgaac gggcagcgaa aatgcagtgg gaacgcctgc aacgcggcgt tgcatcattc
     2761 tcgctacaac tggcggaagg gcgggcagat ctctacacag aaatgcctgt gaaggtcagt
     2821 ggctttaaac agccgctaga tgatgcggaa tggaccatta cgactctgac gcatactgtc
     2881 agcccggata acggttttac gaccagtctg gagcttgaag tgaggattga tgatttcgaa
     2941 atggaatgat tcttcgcaat ggagaacttt taagtttgca aaatggaata atgcggtatc
     3001 attattgtga atttagcaaa aatggggaga actcgaaaaa tgatgatttg cccactgtgt
     3061 ggaagtgccg cccatactcg cagcagtttt caggtatctt cattgaccaa agagcgttac
     3121 aaccagtgcc agaacattaa ctgcagccat acttttgtta cccatgaaac ttttgttcgt
     3181 tcgattgcaa cgccaaaaga gtcaaatccg gttcagccgc atccaatgaa atcaggacag
     3241 gtggcgctct ctctttgacg ctgccgccat tttgtcgcca tcgttaaaaa acagtgcttc
     3301 taacatcatg attttaaaag acataaattt cagacaacaa aaaacccatc aaccttgaac
     3361 cgaaatggcg gggttgatgg gctccacaaa atggggacat caaagaaaag cagtggcact
     3421 aattaagact gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt tcattttcag
     3481 ggcaacttca gttttatcct aatcctggcc ataccatgac gatgattgtc cctgccagcg
     3541 tcagcaggac gttggcgatt gcgtaggtgc ccgcatagcc cagcgcaggg atgttactgc
     3601 gagctgtatc actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc
     3661 cgaacaacag tgcgcggttc attcgcaata cataagcacc gaacaagaaa cagataacca
     3721 cgggcacaag actgacaatt aatccggcaa tcaacatctg accgccaatc gcgcccaggc
     3781 cgttattaat accgctaccg gcgctcagac caacgcctgc cataaacacc atcaagccga
     3841 actctttcac catgtttaat gcaccttgcg gaatgtaacc gaaggtcggg tggttagcac
     3901 gcataaagcc cagcataatt ccggcgaata acaacccggc agcgttcccc atgccgaaac
     3961 tgaatgtgct gaactggaag gtgatcatcc cgatcatcag cccaataaca aagaaggcgc
     4021 aaaatgccag caggtcagtg acctggctgt gaatcgagat aaagccgatg cgatcggcga
     4081 tggtttttac gcgacgggca tcaccgctga cttgtaaaac gtcacctttg ttaagcacga
     4141 cgttgtcatc tatcggcatc tcaatctggc tacgaatgac gcggttaagg aagcaaccgt
     4201 gatcggtcaa cttcagttgt gcgagacgtt tacctacagc gttatggttt ttaacgacca
     4261 cttcttcagt gacgatacgc atgtcgagaa ggtcacgatc gaaaacttct ttaccgttac
     4321 ggaagctggg atcgagtcgg gcatgggcgt cgggatagcc taccaacgct atttcatcgc
     4381 ccatttgtag cacggcatca ccgtctggat ttgccagaat cccgttacgt cgaatacgtt
     4441 caatgtagca gccggtttgt cgataaatac ccagttcacg cagatttttg ccgtcggtcc
     4501 aggccaccag ctccgggccg acgcgatagg cgcggatcac cggtaaataa accttacggt
     4561 tggcatcagt gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct
     4621 gatgctgcaa tttcggcaag taacgcgcac caacaatcaa actcaccaga ccgattaaat
     4681 aggttaaggc atacccgagg ctcagattat ccagtgccag tgagagctgc ctgctttcca
     4741 tgccggaatg acgcagtgta tcgccagcac cgaccagaac cggtgtcgac gtcatagagc
     4801 ctgctaacat accggccgtc aggccaatat cccagccaaa cagcttacct aaccctaagg
     4861 cgatcaccag cgcactgcca accatcacca gtgctaacat taggtaattt ttcccatcgc
     4921 gaaaaaaaat ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa ataaacagca
     4981 taaagccaag attaagcgca tcggtgttaa tgctgaaatg ttgttggcct aataacagcg
     5041 atacgactaa aacgccaatg gaattaccca gttggatcga accaagtcgt aactttccga
     5101 gacatagccc aagcgcgagg accacaaata ataacagaat gtaattccca tttaacaatt
     5161 cggcgacgtt tatattcacg gaggctaact tcttgtttac tagtaagctg ttgaaagaaa
     5221 tggtaattta cgataatgtt ttttaccaga attcagggcg cagattcatt cagcgcacct
     5281 aaacgatagt aaagtaacaa tatattttac tagtgtaatc acattaggta tcaacggcta
     5341 tatgaattgc gttggcctat attagcatgg aatgcgaagc ggctttatct tactgaacgc
     5401 cacactggcg aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt gaaacataaa
     5461 caacgttggg cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg cctttttctg
     5521 gcgacgcaca tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg cttgctgttt
     5581 ttcattcttc ctggagcagt tgccagtttc ttttcacagc gtagagaagt cctgaaacct
     5641 ctatttggcg caatgctggc ggcaccctgt tcaatgctca ttatgcggct gtttttttca
     5701 ccgacgcgct cattctggca agagctggca tggttactaa gcgcggtgtt ctggtgtgcg
     5761 ctgggggcac tgtgtttctt atttatcagt agtttgttta aaccacagca cagaaaaaat
     5821 cagtaaagcc ctcaacgcga gggcttgtca gacgatcagg cgtccagatt ttctttcacc
     5881 catgcagcaa aatcggtata gccgccgata tgttgctgat cgacaaaaat ctgcggcacg
     5941 gtttctacgg gtttacctgc cttttgttgt agatcttctt tagtgatccc ttccgcacga
     6001 atatctacat actgatactg aaaatcatcg cgttcattgc tcaatttctc agccagatct
     6061 tttgcacgca cacagtaagg gcaacccgaa cgaccaaaaa taacggtttg cattatttct
     6121 ctcctcatag atttatgcct gtaatgatca cgctaaaatg tattcgctga aagtaggttt
     6181 aacctgttgc attaattgct aaaagctata actgttaaac acaatacagt gaaaagtttt
     6241 agactgaagg ctcactttgc agagggaagc gtatgcgcgc gatcggtaaa ttgcctaaag
     6301 gcgtgttgat actggaattt atcggaatga tgctactggc ggtggcgctg ctgtcggtaa
     6361 gcgactccct gtcgctgcct gagccatttt ctcggccaga agtgcagatt ctgatgattt
     6421 ttctcggtgt tttgctcatg cttcccgctg cggtggtggt tattcttcag gtggcaaaac
     6481 gtcttgcccc acagctgatg aaccgtccac cgcaatattc acgttcagaa agagaaaaag
     6541 ataatgacgc caaccattga acttatttgt ggccatcgct ccattcgcca tttcactgat
     6601 gaacccattt ccgaagcgca gtgtgaggcg attattaaca gcgcccgtgc gacgtccagt
     6661 tccagttttt tgcagtgtag tagcattatt cgcattaccg acaaagcatt acgtgaagaa
     6721 ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt ctgggtgttc
     6781 tgtgccgact ttaaccgcca tttacagatc tgtccggatg ctcagctcgg cctggcggaa
     6841 caactgttgc tcggtgtcgt tgatacggca atgatggcgc agaatgcatt aaccgcagcg
     6901 gaatcgctgg gattgggcgg ggtatatatc ggcggcctgc gcaataatat tgaagcggtg
     6961 acgaaactgc tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg ccttggctgg
     7021 cctgcggata atccggatct caagccgcgt ttaccggcct ccattttggt gcatgaaaac
     7081 agctatcaac cgctggataa agacgcactg gcgcagtatg acgagcaact ggcggaatat
     7141 tacctcaccc gtggcagcaa taatcgccgg gatacctgga gcgatcatat ccgccgaaca
     7201 atcattaaag aaagccgccc atttattctg gattatttgc acaaacaggg ttgggcgacg
     7261 cgctaaaacc gccacgtcga tgtatgatac gcgggctttt gaccaggtct gacagagagg
     7321 tgcagggtga aaattgccat attgtcccgg gatggaacgc tctattcgtg taagcggctg
     7381 cgtgaagccg ctatacagcg cggtcacctg gttgaaattc ttgatccgct ttcttgctac
     7441 atgaacataa atcctgcggc gtcttctatt cactacaaag gccgcaagtt accccatttt
     7501 gacgcagtga tcccgcgtat tggcaccgcc attacctttt atgggacggc ggcactgcgc
     7561 cagttcgaga tgctggggag ctatccgctc aatgagtcgg tcgccattgc ccgggcgcgt
     7621 gacaaattgc gttccatgca actgctggcg cgtcagggca tcgacctgcc tgtcacgggc
     7681 attgcgcatt cgccggatga taccagcgat ttaatcgaca tggtcggtgg tgcgccgctg
     7741 gtggtcaagt tggttgaagg cacgcaggga attggcgtcg tgctggcgga gacgcgtcag
     7801 gcggcggaaa gcgtgattga cgctttccgc ggtctgaacg cgcatattct ggtgcaggaa
     7861 tatatcaaag aggcgcaagg gtgcgatatc cgctgtctgg ttgttggcga tgaagtggtc
     7921 gctgcgattg aacggcgggc gaaagagggc gattttcgtt ccaatttgca tcgtggcggc
     7981 gcggcaagcg tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa agccgcgcga
     8041 acgatggcgc tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg gccgttggtg
     8101 atggaagtga atgcgtcgcc ggggctggaa ggaatagaaa aaaccactgg tatcgacatc
     8161 gcgggtaaaa tgatccgctg gatcgaacgc tacgctacga cagaatattg cctgaaaacg
     8221 ggtggttagt cgcaatcaca ttactgatca tggttttgcc tgcgcttttt gcgtaagctg
     8281 tgccggtctt tttatcgaaa gaggttgtac aaaattatga catcgctggt cgttcctggt
     8341 ctggatacgc tgcgtcaatg gctcgatgac ctggggatga gtttttttga atgtgataac
     8401 tgtcaggctc tgcatctgcc ccatatgcag aatttcgacg gtgtctttga tgccaaaatc
     8461 gatctgatcg ataacacgat cctgttttct gccatggcgg aagtccgacc ttcagccgta
     8521 ttgccgctgg cggcggattt atctgccatc aatgccagtt cgctgaccgt gaaagcattt
     8581 cttgatatgc aggatgataa tctgccaaag ctggtggttt gccagtcttt atccgttatg
     8641 cagggcgtaa cctacgagca gtttgcatgg ttcgtgcgtc agagcgaaga gcagatttcg
     8701 atggtcattc ttgaagctaa tgcccatcaa ctgctgttac cgactgacga tgaagggcaa
     8761 aacaacgtta ccgaaaacta tttcctccac tgataactcc tttcgagcac gcagtcgctg
     8821 gtgcagtgac tgcgcactgc aaaattatct gctgttttta accttttctt aaagattatt
     8881 tcacttctct tgtgccgatt tggctttatc acatagagca aatacgcata acaatttgtt
     8941 aaataccgtt ttttaatccg agctatagtc tcaaaccctg gctaaagtta ttcttgcgat
     9001 gcttttatat agcgagcagt gctggccggg agagagttat cttttcttac accgcgccga
     9061 taaaaaatat gcacgtttat tgcatatctt ttagtatgac aacttttgtt cgtttgttaa
     9121 cgaactttca gaaggaaaga gatatgaccg ccttaaataa aaaatggctt tcgggtctgg
     9181 ttgcgggtgc tctgatggcc gtctctgtcg gcacgctcgc ggctgaacaa aaaacactcc
     9241 acatttataa ctggtctgat tatatcgccc ctgacacggt ggccaatttt gaaaaagaaa
     9301 ccggtattaa agtcgtctac gatgttttcg actctaacga agtactggaa ggcaaattaa
     9361 tggcgggtag caccggcttt gacttggtgg ttccatctgc cagctttctt gaacgccagt
     9421 tgactgcggg agttttccag ccgctggaca aaagcaaatt gccggagtgg aagaatctcg
     9481 acccggagct gctgaagctg gtcgccaaac acgatcccga caataaattt gctatgccct
     9541 atatgtgggc gacgaccggg attggctata acgttgataa agttaaagct gtgctgggcg
     9601 aaaacgcgcc cgtcgatagc tgggacttga tcctcaaacc tgaaaatctg gaaaaactga
     9661 aaagctgcgg tgtctctttc ctggatgcgc cagaagaagt ttttgctacc gtgttgaatt
     9721 atctcggcaa agatcccaac agcactaaag cggatgatta caccggacag gcaaccgatc
     9781 tgctgttaaa gctgcgcccg aacattcgtt atttccattc atctcaatac attaacgacc
     9841 tggcaaacgg cgatatttgc gtcgctatcg gctgggcagg tgatgtctgg caggcgtcaa
     9901 accgcgcgaa ggaagcgaag aatggagtga atgtctcgtt ctcgattcca aaagaagggg
     9961 cgatggcgtt ctttgatgta ttcgccatgc ctgcggatgc caaaaacaaa gacgaagcct
    10021 atcagttcct gaattacctg ctgcgcccgg atgtagtagc gcatatttcc gaccatgtgt
    10081 tctatgccaa cgccaataaa gcagccacgc cgctggtgag tgcggaagtc cgtgagaacc
    10141 ggggtattta tcctccggcg gacgttcgtg cgaagctatt cactctgaaa gtacaggatc
    10201 cgaaaatcga ccgtgtgcgc acccgcgcgt ggaccaaagt gaagagcgga aaataatccg
    10261 cagccttaga tgcagggcgg gcgcaccaca cccgccggca attcgcacca tcatggtgcg
    10321 cttgcacaca ttcaatgccg gagagcagcc gtgaatgacg ctatccctcg cccgcaggcg
    10381 aaaacccgta aggcgctgac gccgctatta gaaatccgca acctcaccaa atcctacgat
    10441 ggtcagcatg cggtcgatga tgtcagcctg accatctaca aaggtgaaat cttcgcgctg
    10501 ctgggcgctt ccggctgtgg caagtccacg ctgctgcgta tgctggcagg ttttgaacaa
    10561 ccttctgccg ggcagataat gctcgatggc gtcgatttgt cacaggtgcc gccttacctg
    10621 cgccccatca atatgatgtt tcagtcttac gcgctgtttc cgcatatgac cgtggaacag
    10681 aacatcgctt ttggcctgaa acaggacaaa ctaccgaaag cggaaattgc cagtcgggtc
    10741 catgagatgc tcggcctggt gcacatgcag gagttcgcca aacgcaaacc gcatcagctt
    10801 tccggtggtc agcgacagcg tgtggcgctg gcccgaagcc ttgccaagcg cccgaaacta
    10861 ttactgctcg acgagccgat gggcgcgctg gataaaaagc tgcgtgaccg gatgcagctt
    10921 gaagtggtgg atattctgga gcgtgtcggc gtgacctgtg tgatggtcac tcacgatcag
    10981 gaagaggcga tgacgatggc gggacgtatc gccattatga atcgcgggaa atttgtccag
    11041 attggcgagc cagaagagat ctacgaacat ccaaccaccc gctacagtgc tgaatttatc
    11101 ggttcggtta atgtctttga aggtgtactc aaagagcgtc atgaggatgg gcttgtgctt
    11161 tattcgcctg ggctggtgca tccactgaaa gtcgacgcgg atgcctcggt ggtcgataac
    11221 gtgccggtac atgtggcgct gcgcccggaa aaaatcatgc tttgcgaaga gccgcccgcc
    11281 aatggttgta acttcgcggt gggggaggtg atacacattg cctatctcgg cgatctttcg
    11341 gtgtatcacg ttcgtctgaa aagtgggcag atgattagcg cccagctaca aaacgcccat
    11401 cgtcatcgta aagggttacc gacctggggc gacgaagtgc gtttgtgctg ggaagtggac
    11461 agctgtgtgg tgctgacggt ttaaggagca aagatgaata cacttgaacc tgctgcccag
    11521 tcgaaaccgc cgggcggatt taagctgtgg ttgtcgcagc tgcaaatgaa gcatgggcgc
    11581 aaactggtca ttgcgttgcc atatatctgg ttgatcttgc tgtttctgct gccatttctg
    11641 attgtcttta aaataagcct ggcggagatg gcgcgcgcta ttccacctta taccgaactg
    11701 atggagtggg ctgacgggca actgtccatc actcttaatc tcggtaattt tctgcaactg
    11761 accgacgatc cgctctattt cgatgcttat ctccagtcgc tacaggtggc tgcgatttcg
    11821 acattttgct gtttactgat cggctatccg ctggcgtggg cggtggcgca cagtaagcct
    11881 tcgacccgta atattttatt actactggtg atcctgccgt cgtggacctc gtttctgatc
    11941 cgcgtttatg cctggatggg aatattaaaa aacaacggtg tgctgaataa ttttctgctg
    12001 tggctggggg ttatcgatca accgctgacc attcttcata ccaatctggc agtttatatt
    12061 ggcattgttt acgcctacgt gccgtttatg gtgctgccga tttataccgc gttgattcgt
    12121 attgattatt cgctagtgga agcagcgctg gatctcggtg cacgaccgct gaaaacgttc
    12181 tttactgtga tcgtgccgct gactaaaggt gggattattg ccggatcgat gctggtgttt
    12241 atcccggctg tgggcgagtt tgtgatcccg gaactgctcg gtggcccgga cagcatcatg
    12301 atcgggcgcg tgctatggca agagtttttc aataaccgcg actggccggt ggccgcggcg
    12361 gtagcgatca tcatgttgct gctgctaatt gtgccgataa tgtggttcca caaacaccag
    12421 caaaaaagcg tgggagaaca cggatgaata atttaccggt agttcgttcg ccctggcgga
    12481 ttgtgatttt gctgctgggc tttacctttc tctacgcgcc aatgctgatg ctggttatct
    12541 attcgtttaa cagctcgaag ctggtgacgg tgtgggccgg ctggtcaacg cgctggtatg
    12601 gtgagttatt gcgcgatgac gcgatgatga gtgcggttgg tttaagcctg acaattgctg
    12661 cctgtgcggc aacggcggcg gcgatcctcg ggactattgc ggcggtggtg ctggtgcgct
    12721 ttggcaggtt tcgcggatca aatggctttg cctttatgat cacagcgccg ctggtaatgc
    12781 cagatgtcat cacgggcttg tcgctgttgt tattattcgt cgcgcttgct catgccattg
    12841 gctggcctgc ggaccgcggt atgcttacca tctggctggc gcatgtcact ttctgtacgg
    12901 cttatgtggc ggtcgttatt tcgtcgcgtc tgcgggaact ggatcgctcg atagaagaag
    12961 cagcgatgga tctcggtgcg acgccgctga aagtgttttt cgtcattacg ctaccgatga
    13021 tcatgcctgc gatcatttct ggctggttac tggcttttac tttgtcgctt gatgatctgg
    13081 tgatcgccag ctttgtttcc gggccgggag ccaccacgtt accgatgctg gtcttttcca
    13141 gcgtgcggat gggggtgaat ccggaaatca acgcccttgc aacgttaatt ctcggtgcgg
    13201 tcggaattgt cggatttatc gcctggtatc tgatggctcg cgcagaaaaa cagcggatac
    13261 gcgatatcca gcgtgcaaga catggctgaa gacactaaaa tttgccaacc tggctacata
    13321 atgccgcgca tgccgcggca ttgttttcat ggaagacgaa acgttgggat tttttaagaa
    13381 aacatcttca tctcatgctc gcctgaatgt gcctgcgctg gtgcaggtag cggcgctcgc
    13441 cattattatg atccgtggcc tcgacgtgct gatgattttc aatacgctgg gcgtgcgcgg
    13501 tattggcgag ttcattcatc gcagcgtaca aacctggagc ttaacgctgg tatttttaag
    13561 cagtctggtg ctggttttta tcgagatctg gtgtgcgttt tcactggtga aagggcgtcg
    13621 ctgggcgcgc tggctatatc tgctgacaca aatcaccgcc gcaagttact tgtgggcggc
    13681 ttcgctgggg tatggttatc cggagctgtt cagcattccc ggtgaatcaa aacgtgaaat
    13741 cttccatagc ctgatgctgc agaagctgcc ggatatgctc atcctgatgc tgctgttcgt
    13801 tccctcgacc agtcggcggt tcttccagtt gcaataatgt gtataatcgt cgcccctgat
    13861 gatgtgaagg tcaatgtatg cagtgcgcac tttacgacgc gggtcgctgt cgttcctgtc
    13921 agtggataac gcagccgatt ccagagcaac tctccgctaa aaccgccgat cttaaaaatc
    13981 tgctcgccga ttttccggtt gaggaatggt gcgcgccggt gtcaggcccg gaacaagggt
    14041 ttcgtaataa agccaaaatg gtggtgagtg gtagcgttga aaaaccactg ctcggtatgc
    14101 tgcatcgcga tggcacgccg gaagaccttt gtgactgccc gctttatcct gcctcttttg
    14161 cgcccgtttt tgcggcgcta aaaccgttta tcgcccgtgc ggggttaacg ccctacaacg
    14221 tggcgcgtaa gcgtggcgaa ctgaaataca ttctgctgac tgaaagccag agcgatggcg
    14281 gcatgatgct gcgctttgta ctgcgttctg aaaccaaact ggcacaactg cgtaaggcgc
    14341 tgccgtggtt acaggaacaa ctaccgcaac tgaaagttat taccgtcaat attcagccgg
    14401 tacatatggc gattatggaa ggggagacgg agatctacct gactgaacaa caggcgctgg
    14461 cagagcgttt taatgacgta ccgctgtgga tccgtccgca aagtttcttc cagactaatc
    14521 cggcggtcgc cagccagctg tacgctaccg cgcgcgactg ggtacggcag ctgccagtta
    14581 aacatatgtg ggatctgttc tgcggtgtgg ggggctttgg tttacactgc gcgacgcctg
    14641 acatgcagtt aaccgggatc gaaattgcgc cagaggccat tgcctgtgca aagcagtcag
    14701 ccgctgaact gggcttaact cgtttacaat ttcaggcgct ggactccact cagtttgcca
    14761 ctgctcaggg ggaggtgccg gagctggtgc tggttaaccc gccgcgccgc ggcattggta
    14821 aaccgctgtg tgattatctc tcaacgatgg caccgcgttt tatcatctac tccagctgta
    14881 acgcccaaac tatggcgaaa gatatccgcg aactgcctgg gtttcgcatc gaacgggtac
    14941 agctttttga tatgttcccg cacaccgcgc actatgaagt gctgacgctg ctggtgaagc
    15001 aataaaaaag ccgcaggtgc ggcttcagat tgctgatcag gtgcgctttg ttcatgccgg
    15061 atgcggcgtg tacgccttat ccggcctaca aaatcatgct aaatcaatac attgcagagt
    15121 tgatgtgggc cagataagcg cagcgcatca ggcaatctta cttttgtcat cagtctcaat
    15181 tactgcggga accactggtc attgattttc tgatatgtac cgtcagcttt aattgctgcc
    15241 agcgcgttat tcagtttttc cagcagagct ttgttatccg gacgtacagc gatgcccaga
    15301 ccggtgccga agtattgcgg atcggtcact ttttcagtag caacgcccag ttgcggattg
    15361 gttttcagcc attcgtttac caccgctgtg tcaccaaata ccccatcaat acgaccattt
    15421 ttcagatcga taaaggcatt ctgataactg tcataggaga cagttttcac ttcggggtgc
    15481 tgatcctgaa tatatttctg gtgcgtggtg ccgttttcca tcccaatacg tttgcctttc
    15541 agatcggcaa acgttttgta ggtatctttt ttggcaatca cgacggctga gttttcatag
    15601 tagggcgtgg taaacgatac ctgcttgcta cgctcgggcg tgatatccat accggagatt
    15661 acggcgtcat attttctgaa tttcagtgac gggatcaggc tgtcgaacgc gtgattagta
    15721 aaagtacatt ctgcctgcat ttgtttgcac aaggcttttg ccagatcgat atcaaagccg
    15781 acaatctcat tattagcacc tatagattca aagggtggat aggtggctga aacgccaaaa
    15841 ttgattttct ctgcggcaga agcaccgaaa gtaaaggaag caagtaaagc ggcaagaact
    15901 aactttttca tgatggaact cccgtctgtc aatcttatga tttttggccg tgtctgcggc
    15961 atgggataac aatgccatca agtgaattta tatgcaataa atatgattaa ataatttaaa
    16021 tgaaataaaa aacgacggat aacttagtga gttgtccgtc ttcattataa gaatttatgc
    16081 actatgtagg ccggataagg cgtccacgcc gcatccggca caggcaccgt gctgatgtct
    16141 gatgcgacgc tggcgcgtct tatcagacct acaaaccccc cggcgaatgt acgcagctac
    16201 attaattccg ccgttcgaat gccagtgctt tgcgctcgat cagacgcatc atcagcgtca
    16261 gcaggccgtt gacgaccagg taaataatcc ctgccgcacc gaacaccatt acatcgtagg
    16321 tgcgtccgta caacaactgg ctgtatccca tcacttccat cagcgtaatg gtgtaagcca
    16381 gagaggtact tttgaacacc agcaccactt cgttggaata agaagagagc gagcgtttaa
    16441 aggcatacgg cagcaggatc gccagcgtat cttttttgct cattcccagg gcgctacagg
    16501 actgccactg accttctggg atcgcacgaa ttgcaccgta aaacagctgc gtggtatacg
    16561 ccgcactgtt cagcgataac gcaatcagcg cacataacca cggttctgac aacaaatgcc
    16621 acagtgccgg atactcctgc aaagtcggaa actggcccgg cccgtaataa atcaggaaga
    16681 tctgcaccag cagcggcgta ccggtaaaca gcgtgatata accccgcacc agccacacca
    16741 gcaccggcgt tttcagcgtc aggatgatgg taaaaatcaa tgccagaatc agtgccacaa
    16801 tcagcgaggc aacggttagc gtcaggctgg tgtgtagccc tttcatcagt tcgggtaaat
    16861 actcaaacat tagctgggcc tccgctcaaa acgtgtcgcg cgcaggtcaa tgcgtttgag
    16921 aatgtactga ctgagcaggg tgatcaccag gtaaatcgcc gccgccacaa tataccaggt
    16981 aaatggttcc tgggtacgag tagcgatgct ttttgtttgc agcattaaat cattcacact
    17041 aatcaaactg accagcgcgg tatctttcag cagcaccagc cactggttac cgaggccagg
    17101 cagcgcatga cgccacatct gcggcatcac cagacggaaa aagatagccg atttcgacag
    17161 ccccagcgcc tgaccagact cccactgacc caccggcacc gctttcaacg cgccccgaag
    17221 cgtttgcgag gcataggcgg catacagcag tgacagagcg atgacaccac aaaggaacgg
    17281 gctaacgtcg aagttctcaa tgtccatctg cactgggatc tgcacgaacc caagattgat
    17341 agtgaagcca tccgaaagcg tcagcagcag ctgcgaggag ccaaaataga taaacagcac
    17401 caccagaatt tctggcaggc cacgcagaat ggttaccagc gctgaacctg cccacgcgac
    17461 aggacgccat ttttccgact cccataccgc aaagaacatc gccagcgcca gcccgacaat
    17521 caatgcacaa acggcaaggc cgacggtcat cccggcggcg cttgctaaag gaaaaaattc
    17581 attcatcagg aattacttct ggaaccattt gttgtagatg gtttcgtaag tgccatcttt
    17641 cttcactttt tccagcgcag tgttgagttt ctgctgcagc tcagtgttgc cctgacgtac
    17701 cgcgatgccg aggccagtgc cgaagtaatc tttatcggtc actttgtcgc ccaccgccgc
    17761 cagtttcggg ttatctttca gccactcagt gaccactgcg gtgtcaccga agacgccgtc
    17821 gatacgcccg ttttgcagat ccagttttgc gttctggtag ctgtcatacg gaacggtagt
    17881 gatttccggg tgcttatcca taatgaattt ctggtgtgtc gtcccgttct gtacgccgac
    17941 ttttttgcct ttcagctgat caacactggt gtatttgcct tgctgaccca caaacagggc
    18001 agagttgtca tagtacgggg tggtaaacag cacctgcttt tcacgctccg gagtgatatc
    18061 catgcccgcc atcacggctt ctacgcgacg gaatttcagg cttgggatca ggctgtcaaa
    18121 cgcctggtta gagaaagtgc aggttgcatc aatctcttta cacagcgctt gtgccaggtc
    18181 gacgtcaaaa ccaacgatct ggttgtttgc atcaatcgat tcaaacggag gataggatgc
    18241 ttcggtagca aaacgaatgg tttcggcagc tgtggcggaa agactaaaac ctgcaattaa
    18301 cgcggcaatc agaacttttt tcattgttgt tatcccgaat cttagtgaga gagatagttt
    18361 ttaaatgctt cggtttgcgg ctcggtaaag cagctcgcgt cgccttgttc tacgatatga
    18421 ccattttcca tatacaccac tcggctggcg gttttacgcg ccacttcaac ttcgtgggtg
    18481 acgatcacct gggtaatatt cgtttctgcc agctcacgaa tgatgctgac gatttgtgcc
    18541 gtaatttccg ggtccagtgc ggcggtcggt tcatcgaaca gcagtacctg cggttccatc
    18601 atcaacgcac gggcaatagc aacacgctgc tgctgaccac cagaaagatg cagcgggtaa
    18661 cgatcgctat aaggtttgag acgcagacgt tccagcagtt tttctgcacg ggccagcgcc
    18721 tgatctttac tcaaccccag tacacggcag ggcgcttcaa tcaggttttg ctgcacggtc
    18781 agatgcggcc acaggttgta ttgctgaaac accatgccaa cgttacgacg caaatcgcga
    18841 atcgctttgt cagagggtgt tttggtgaaa tcgaaatggt tgcctgcaat gttgagcgta
    18901 ccggagcgcg gcatctcaag cagattgagt acacgcagca gcgagctttt acccgcgccg
    18961 ctggggccaa gtaacaccag cgtttcgccc tgtgggcaat ccagcgtgat atcgaacagc
    19021 gcctgatgcg cgccgtagaa gcaattaatg ccgtttaatt gaatactcat tgacactcgt
    19081 atactggcag tctgatagct attgaggtcg aagatagtac ctttgacaga ataattatgc
    19141 aatatttctg ctttaaaagt taaaatcaaa gcgcattatt caataaacat agcacaaaat
    19201 aacgggggcg gtggtcggcg agcataaatg tcggcattcc tcacgaaatg ccggacaatt
    19261 tacggggttt attggttgat caaggcgtta gcgattctcg atggactgac ggagcgtacc
    19321 cgccgtggca tgaacgctgc cgcctaagta gcgtacatcg tcgatcaccc aacactggcc
    19381 ttcctgaatc attaacactt catcctgcca gctctgatca ccctgtttta gatccacacg
    19441 cagaggaata tttcgcgcat cacgattcgg gattgttgag gcactggcaa cacgggcgct
    19501 ctctggcaag gttgtgcgac tggagaatgg atcgttggtc agtagttccc gatggttatt
    19561 atcccgggag gcatcgctaa gcagtgtcgc cagtttgtcg ctcagataag ggcgcaaggc
    19621 ggtgatgtcg ttgctgcggt gcaaaatgcg gtagtcataa aattgctggg ccacgttacc
    19681 cgggcctcct tcaacgcaag gaccactgcg tgtgccgtta tctttataag ctggagtgac
    19741 tgtggtgcag gcactgagga gcagtgcgca ggggataagc attgtcaatt tgctgtagcg
    19801 cataatgatt tccttataag cgatcgctct gaaagcgttc tacgataata atgatatcct
    19861 ttcaataata gcgtatcagt ctgataatgc ttttgagatc gaaggcttag caaacaagga
    19921 gatcgatcat gcaattttct acaaccccaa ctctggaagg ccagaccatc gttgaatatt
    19981 gcggtgtggt gaccggcgaa gcgattttag gtgccaatat tttccgtgat ttctttgccg
    20041 gtatccgcga tatcgttggc ggacgttccg gtgcgtatga aaaagaactg cgtaaagcac
    20101 gggagatcgc ctttgaggaa ttaggctccc aggcgcgggc gctgggggcc gatgccgtcg
    20161 tcggtattga tatcgactac gaaacggtcg ggcaaaacgg cagtatgctg atggttagcg
    20221 tcagcggtac ggcggtgaaa acgcgtcgat gagaagagtc ttctggctgg tcgctgccgc
    20281 tctgttattg gcagggtgtg caggcgaaaa aggcattgtc gagaaagagg gatatcagct
    20341 tgatacccga cgccaggcgc aggcggcgta tccgcgcatt aaagtgctgg tgatccacta
    20401 caccgcagat gattttgata gctcgctggc gacactgacc gataagcagg tcagctcgca
    20461 ttatctggtc cctgcggtac caccgcgata caacggtaaa ccgcgcatct ggcaactggt
    20521 gccggaacaa gaactggcct ggcatgcggg gattagcgcc tggcgcgggg caacgcgcct
    20581 taacgacacc tctattggca ttgagctgga aaaccgtggc tggcaaaaat cggccggagt
    20641 gaaatatttt gccccgtttg aaccggcaca gattcaggcg cttattccac tggcgaaaga
    20701 tattattgcc cgttatcaca tcaagccgga aaacgtagtg gcacatgcgg atatcgcacc
    20761 gcagcgcaaa gacgatccgg ggccattatt tccctggcag caactggcgc agcaggggat
    20821 tggtgcctgg ccggatgcgc agcgggttaa cttttacctt gccgggcgcg cgccgcacac
    20881 accggtagat accgcgtcat tgctggagct tttggcgcgc tacggttatg acgttaaacc
    20941 tgatatgaca ccgcgcgagc agcggcgcgt gattatggca ttccagatgc atttccgccc
    21001 gacgttatat aacggcgaag cggatgcaga aactcaggcg attgccgaag cattgctgga
    21061 gaaatacggg caggattagc gcggcagttt tccgtggtcg cgtagccagg cggcagtttt
    21121 ctcgatacct tcatccaggg tgatgaccgg ctgataacct aactcttcct gcgcacgcgt
    21181 aatatccagc gtaaagtcaa aattcaactt ggagacgccg tagtgggtca gcggcggctc
    21241 ttttgctgac ttgcggaaac gctccatgct gcgggcgatc atatccagca tcgggtaggg
    21301 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgca
    21361 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    21421 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    21481 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    21541 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    21601 gcgcgtttgt ggattcgcct gcgaaaacat attgatcact tcttcgctgg ctgctttgct
    21661 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    21721 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    21781 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    21841 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    21901 cactttagct tgtgatgaaa ccagctcggt cagatccgcc ggaacaaact ctgcgcccat
    21961 tttttccagc aatttgccca ttgcctcgtt gcgaccggtc gctcgcacgc tgatgccttt
    22021 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    22081 cttcatatca atccactgtt gttgagaaaa taacgtgcgc attcttccgt gatttccccc
    22141 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    22201 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    22261 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    22321 atagtacgat agtcgccttt atcttccagg ctaaaacaca gtcgtcccag ccccggcgct
    22381 ttcatgccaa ataacaacgt aagttgtttt tccggttcaa cgacaatcac tttccagcta
    22441 tctaccgcat cgccagtctg taaatattcg cgctccgggc ggcctttcgc cagcttatga
    22501 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    22561 tctttaccgc cgatttggtt cactacctgc cataaagcag caaggctggc ggacgtttta
    22621 acggtaaacc ccgcctgttt ggcaaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    22681 tgagcgtcgt agccccagtc gctggagttg accagttttt cctcctcttt caacgtgcta
    22741 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    22801 tccgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    22861 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    22921 aaccagcggc gcttgccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    22981 acctctggtc cggcggcttc gaagatgcgg tgttcgctgg ccggatgatc taacaacgcc
    23041 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    23101 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    23161 cctgcgccaa cgataattcc ggctcgaagt tcggtcacag gtacattcgc ttcacgaaga
    23221 atgtccgccg tagcctgacg agcacgcaga tgatccgact gctcatgtgg cggggcctgc
    23281 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    23341 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    23401 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    23461 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    23521 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    23581 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    23641 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    23701 caggcggaca atcggcgagg cgttaatcag cacgtttctc actttcatgt attcgcctaa
    23761 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    23821 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    23881 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    23941 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    24001 accgacgagt aatgaaccga caggcgtccc aagacctttc gacaggcaaa tggtgaacga
    24061 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    24121 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    24181 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    24241 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    24301 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    24361 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    24421 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    24481 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    24541 aacgtcgtcc ccaaccgggg cagccatcat cgcttcgagc atggcgcggc tcggtcgggt
    24601 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    24661 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    24721 gattgcgcgc aacatataca agctaaatcc tttggcctgt tcgagtttga tctgcggtgg
    24781 aatggctaac tcttctttgg cgaccaccac atccaccagc accggaccgt cgatggagaa
    24841 ggcgcgttgc agggcttcat caacttcaga cgctttttct acacggatac ccgtaatgcc
    24901 gcacgcttcg gcaatgcggg caaagtttgt gtcgtgtagt tcggtgccgt cagtcaaata
    24961 gccaccagct ttcatctcca tcgccacaaa gcccagcacg ctgttgttaa agacgacaat
    25021 tttcactggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    25081 gccatcgccg cacatggcga ccacctgacg ttctggctct gtcgcctgcg cacccagcgc
    25141 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    25201 gttcattttt agataacgtg ccgcccacac cgttggcgta ccaacgtcac aggtgaaaat
    25261 agcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    25321 ctcgctcggt ttagctaaat cgtccagccc tttgcgggcg tcgcggtaat cttccagcgc
    25381 tttatccaga aacttgcgat cggctttttc ttccaccaat ggaagcaatg cacgcagagt
    25441 cgacttgata tcgccgacca gtgccatatc caccttgctg tgagcgccga tgctggctgg
    25501 gttgatatca atctgaatga ttttggcatc ggtcgggtag aaggcgcggt agggaaattg
    25561 cgtgccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaagcc
    25621 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgtt ctttaccgcg
    25681 cagggcatga acaataggcg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    25741 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    25801 cagtttgcgt aactcttctt cttccggcgt cacgactggt tgtggcgcat gataccagtg
    25861 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cctggtaaca cgacaaccga
    25921 aacgccacgg ttaagcaccg ctttgcgcat ggcaatcgcc agtacttgtg ggatctgctc
    25981 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    26041 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    26101 cggaacgtga ttgcggtggc aatcaaacag gccgttgatt aagtgcaggt tgccggggcc
    26161 gcacgatccg gcgcagaccg ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa
    26221 ggccgccact tcttcgtggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    26281 gtcactaaga ccattcagag agtcgcctgt gactccccag atgcgtttca cccctgccga
    26341 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    26401 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    26461 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    26521 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    26581 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    26641 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    26701 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    26761 gaatttcaga ccgctggtcg ccgcttttgc tactggggtg aagaatttct ctttaaagaa
    26821 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    26881 gcaggtcatc acggtacgtg aagctaagtc aggtacacct gccagcagtt ctcgagtgag
    26941 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    27001 acgccactca tcggcgaaaa taacatcctg cggcgtatgc acgttgtaga tcacccgcac
    27061 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    27121 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    27181 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    27241 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    27301 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    27361 ttgcccggcg cgatatgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    27421 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    27481 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    27541 gaacgcaggc cgaatttctc gttcagcacc gccagtaggt ctggtgtcag gaaaccaggt
    27601 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgacg
    27661 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    27721 cccagtttct ctgccagagt gacagccaga ataatcgctg agtaagcatc gttacattga
    27781 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    27841 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgatggt ggcgaaatcg
    27901 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    27961 agtttttcac ggctcaccag atcaatcagc gtatcagcag cgccaagcag cgtctggcga
    28021 ccaaaaccca cggtgataag gtgcggaatt tcgctgtacg ggaagcctgc catctgttgc
    28081 gcctgggtga taaccgcaga gaaatcatca ccgtccagat gacgcacgcc aggccagcca
    28141 acaatgctgc gggtccagat acgatcgtca taagcgctta cggttgggtc gatgatgcag
    28201 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    28261 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    28321 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    28381 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    28441 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    28501 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    28561 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    28621 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    28681 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    28741 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    28801 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    28861 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcgtagaatc gaagttaacg
    28921 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    28981 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    29041 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    29101 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    29161 aaagttatat ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat
    29221 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    29281 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    29341 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    29401 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    29461 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    29521 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    29581 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    29641 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    29701 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    29761 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    29821 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    29881 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gtgccacggc aggcctcgct
    29941 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    30001 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    30061 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    30121 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    30181 cagaaaacat gaaaacccag tccttgcaaa gatgaagtcg aaatgcgcga tgacacacta
    30241 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca taaggagcag
    30301 tagagtattc gttttcattt aaagatattc ttgcgcttta attacaaact gcaccgatgt
    30361 tggtggcgtc aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg
    30421 gctgagggcg cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata
    30481 agtcgagggc accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg
    30541 cggcgattgg cc
//
LOCUS       DADLTD010000026        28752 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLTD010000026 DADLTD010000000
VERSION     DADLTD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.026"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..802)
                     /locus_tag="JN701_000950"
     CDS             complement(<1..802)
                     /locus_tag="JN701_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7363474.1"
                     /translation="MRYPILHKLKDRLNQTWYQIRIGDRLAYISALDAQPDNGLPVLT
                     YHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQLEGYVKNKINLPAR
                     AVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKWDPKSLQFMSVSELN
                     EIRDVFDFQSHTHFLHRVDGYRRPILPSRSEHNILFDFARSRRALAQFNPHVWYLSYP
                     FGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILRTDSLE"
     gene            complement(780..1163)
                     /locus_tag="JN701_000951"
     CDS             complement(780..1163)
                     /locus_tag="JN701_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004980383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAY7363475.1"
                     /translation="MLFTASVSAALPARYMQTIENAAIWAQIGDKMVTVGNIRAGQII
                     AVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSNQNLITWKDTP
                     VYNAPSAGSAPLWGTGGQFALPDFA"
     gene            complement(1258..1698)
                     /locus_tag="JN701_000952"
     CDS             complement(1258..1698)
                     /locus_tag="JN701_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7363476.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(1803..2573)
                     /locus_tag="JN701_000953"
     CDS             complement(1803..2573)
                     /locus_tag="JN701_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7363477.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(2570..3496)
                     /locus_tag="JN701_000954"
     CDS             complement(2570..3496)
                     /locus_tag="JN701_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7363478.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            3605..4267
                     /gene="can"
                     /locus_tag="JN701_000955"
     CDS             3605..4267
                     /gene="can"
                     /locus_tag="JN701_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7363479.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(4308..4844)
                     /gene="hpt"
                     /locus_tag="JN701_000956"
     CDS             complement(4308..4844)
                     /gene="hpt"
                     /locus_tag="JN701_000956"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY7363480.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            5050..7440
                     /locus_tag="JN701_000957"
     CDS             5050..7440
                     /locus_tag="JN701_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY7363481.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(7487..9037)
                     /gene="cueO"
                     /locus_tag="JN701_000958"
     CDS             complement(7487..9037)
                     /gene="cueO"
                     /locus_tag="JN701_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY7363482.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            9203..9550
                     /locus_tag="JN701_000959"
     CDS             9203..9550
                     /locus_tag="JN701_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY7363483.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            9656..10522
                     /gene="speE"
                     /locus_tag="JN701_000960"
     CDS             9656..10522
                     /gene="speE"
                     /locus_tag="JN701_000960"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY7363484.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            10538..11332
                     /gene="speD"
                     /locus_tag="JN701_000961"
     CDS             10538..11332
                     /gene="speD"
                     /locus_tag="JN701_000961"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY7363485.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(11369..11731)
                     /locus_tag="JN701_000962"
     CDS             complement(11369..11731)
                     /locus_tag="JN701_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY7363486.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(11906..14503)
                     /gene="acnB"
                     /locus_tag="JN701_000963"
     CDS             complement(11906..14503)
                     /gene="acnB"
                     /locus_tag="JN701_000963"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY7363487.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            14858..16711
                     /locus_tag="JN701_000964"
     CDS             14858..16711
                     /locus_tag="JN701_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY7363488.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(16782..18206)
                     /gene="lpdA"
                     /locus_tag="JN701_000965"
     CDS             complement(16782..18206)
                     /gene="lpdA"
                     /locus_tag="JN701_000965"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY7363489.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(18531..20423)
                     /gene="aceF"
                     /locus_tag="JN701_000966"
     CDS             complement(18531..20423)
                     /gene="aceF"
                     /locus_tag="JN701_000966"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY7363490.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(20438..23101)
                     /gene="aceE"
                     /locus_tag="JN701_000967"
     CDS             complement(20438..23101)
                     /gene="aceE"
                     /locus_tag="JN701_000967"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY7363491.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(23262..24026)
                     /gene="pdhR"
                     /locus_tag="JN701_000968"
     CDS             complement(23262..24026)
                     /gene="pdhR"
                     /locus_tag="JN701_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY7363492.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            24567..25940
                     /gene="aroP"
                     /locus_tag="JN701_000969"
     CDS             24567..25940
                     /gene="aroP"
                     /locus_tag="JN701_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY7363493.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(25983..26837)
                     /gene="ampE"
                     /locus_tag="JN701_000970"
     CDS             complement(25983..26837)
                     /gene="ampE"
                     /locus_tag="JN701_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY7363494.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(26834..27385)
                     /gene="ampD"
                     /locus_tag="JN701_000971"
     CDS             complement(26834..27385)
                     /gene="ampD"
                     /locus_tag="JN701_000971"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY7363495.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            27473..28366
                     /gene="nadC"
                     /locus_tag="JN701_000972"
     CDS             27473..28366
                     /gene="nadC"
                     /locus_tag="JN701_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY7363496.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSNAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            28569..>28752
                     /locus_tag="JN701_000973"
     CDS             28569..>28752
                     /locus_tag="JN701_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7363497.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHG"
BASE COUNT         6910 a         7852 c         7068 g         6922 t
ORIGIN      
        1 tctccagcga atccgttctt aagatataaa gtcgttttag taacaacgga ttatcccccg
       61 gttttacttt gcctttcatg gttgtcaccg ccaggtgaaa tccggcatcg tttgctgcct
      121 tcacggcttt gtcattaaat ccgccaaacg gatacgaaag ataccagaca tgcggattaa
      181 attgcgccag agcgcggcgt gaacgtgcaa aatcaaacag aatattgtgt tcactacggc
      241 tcggtaatat ggggcggcga taaccatcca cccgatgcaa aaaatgggta tgtgactgga
      301 aatcaaaaac atcgcgaatt tcgttaagtt cagaaacgct cataaattgc agcgattttg
      361 ggtcccactt ctgcgggtga cgtttgatgc gcgaggtaac aataaacgcc gtcgccttca
      421 tgccatattg tttcaacacg ggatacgcat agcggctcac cgatttgagg ccatcatcaa
      481 aggtaatcac caccgctcgc gcagggagat tgatcttatt cttcacgtaa ccttccagct
      541 gcaccatgct cagtgtcgcg tatcccctgt cgcgcagcca ggccatctgg ttattgaaag
      601 cgcgtactga ggtggtcgtc gaagtatggc gaaaacgggt gttttcttca tcgcgcagaa
      661 tatggtgata ggttagcacc ggcaggccat tatcgggttg ggcatccagc gcgctgatat
      721 aggccagtcg atcgccaata cggatctgat accaggtttg atttaacctg tctttcagtt
      781 tatgcaaaat cgggtagcgc aaattgtccg ccagtacccc ataatggcgc actgcccgca
      841 ctcggcgcgt tatagaccgg cgtatctttc caggtaatta agttctgatt actcagcggc
      901 ttgttgaggt cacccaaacc gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc
      961 cctttatcga taaacccttt accaaagcca aaattaaatg cgtaataact tgcggcagtg
     1021 ggctccacgg caatgatttg cccggcccga atattcccca cggtcaccat cttgtcgcca
     1081 atttgcgccc agatcgcagc attttcgatg gtttgcatat aacgggcagg taacgcggca
     1141 ctaacgcttg cggtaaacag catcagcagg atgaataaca gcttgtttat acatgaaaaa
     1201 ataaccgagg gtgaaacaac gcttgtattg tagcaaaagc gccatcagaa tcgcgggtta
     1261 atactcatac aaattatgtt tgaggacaaa aggcggattt ttttgttgtt ggtgccaaag
     1321 actactcacc tccggcgcac ccagttcgac aatacgctcg cgaaactctg aactggtcat
     1381 ggtttcacgg caagccatca tctgttcaat taacggtaac gtgacaccag aaatcacttc
     1441 acaacgggag tgtttatggc ttaataatga agccacgcgg tacggtggcg ctccggctat
     1501 atcggttaag aagatgacac cctcaccaga gtccgcttca tgcagagcat cgcacatcat
     1561 gcggctgagc atattagagc ttaatccgcg ccagaaattc acggcccggc actgaagaag
     1621 tgccccatgt tttttctcca gcgcatccag tatctcttgc gcccggtcat cgtgacaggt
     1681 aattacccaa cctaacatcg ccttcctcct tagccgaaaa tcagtctatc ctgaccattg
     1741 ttgataaagg gtgatagccg tcaaaactcc tctcccccga atccagagga gaggaaatag
     1801 ccttagctac gcaaaccacg tccacgttgg atcagcgacc aacagatcaa ataaaacacc
     1861 acaataaaga ccaccagtac gccaaaggta gtggccagcg gcacatcatt tataccgagg
     1921 aagccgtagc ggaaaccact gatcatataa acgattgggt tcaggtgcga cagcccttgc
     1981 cagaacggcg gcaacaaagt cagggagtaa aacaccccgc ccaaatacgt gagtggcgtt
     2041 aacacaaagg ttggcaccag gctgatgtca tcgaacgttt tggcaaacac gccgttcagc
     2101 aaacccgcaa gggagaatag caccgccgtg agcaccaggg ttaaggcaac gaatacccac
     2161 gaatgcacct gaaacggcac aaaaaacagt gaaattgccg tcaccagaat gccaacaaac
     2221 agaccacgcg ccacgccacc gccgacatat ccggcaataa tgacgtgagt cggaaccggc
     2281 gctaccagca gctcttcaat attacgctgg aacttggcac caaaaaatga tgacgcgacg
     2341 ttagcgtagg cattggtgat caccgacatc atgatcagcc ccggtacgat gaactgcata
     2401 tagctgaagc catgcatatc gccaatacgc gaacctatca ggttaccgaa gataataaag
     2461 taaagggtca tggtgatgac tggcggcacc agcgtctgca cccagatacg cataaagcga
     2521 tggatctctt tcgcccagat gctttttagc gccacccagt aaagatgcat catgcgcgat
     2581 ctccttgttt ttcattaacc agtgaaacaa acagctcttc cagacggtta gctttgttac
     2641 gcatacttaa tacctgaatg ccctgctcac ttaactgcgt aaatacgctg ttgatcccct
     2701 gctcacgcag cacttcaact tccagcgtcg cggtatcgac cagtcgatac tgatagccat
     2761 cgagcttcgg taacgggctt ttcggtgcga gatcgagaat aaaggtttcc aatttcagct
     2821 tcgccagcag cgccttcatc gaggtatttt ccaccagctc accgtgttga ataatgccga
     2881 tattgcggca cagcatttct gcttcttcca ggtagtgtgt agtgagaatg atggtggtgc
     2941 ctttgtcgtt taaatccttc aaaaagcccc acattgagcg gcgaagttca atatccacgc
     3001 ctgcggtcgg ttcgtcgaga atcagtagtt taggttcatg cattaacgca cgggcaatca
     3061 ttaaacggcg cttcatcccg ccagataaca tacgcgcacg ttcgttgcgt tttccccata
     3121 aatcgagttg tttgagatat ttttcgctgc ggatgtacgc ttctttgcgc tccacgccgt
     3181 agtaccctgc ctgattcacc acaatttgct gcacggtttc aaacgggttg aagttaaatt
     3241 cctgcggcac cagtcccaac tgacgtttag cgttcacgac atccttctcg agatcgtaac
     3301 caaatacgct gacccgcccg gaggttttat ttaccagaga gctgataata ccgatagtgg
     3361 tcgatttccc ggccccgttc ggcccgagaa gcgcataaaa atcacccgct tcgacctgca
     3421 aatctattcc acgaagcgcc tgaacgccgc ctggataggt ttttttaagc tgttgaagtt
     3481 ccagtgcaat ggtcataaat ttttacttac cttacgttct tacactttat ctgtggttta
     3541 aatcgtcccg gagttgccct atattagcca aacgtaatta tttggttaca ggtcgttaac
     3601 ctccatgaaa gacatagata cactcatcag caacaatgca ctatggtcaa aaatgctggt
     3661 ggaagaggat cccgggtttt ttgagaaact ggcacaagcg caaaaaccgc actttctatg
     3721 gattggatgt tccgacagtc gcgttcctgc agaacgttta accggtcttg agccgggcga
     3781 actctttgtt caccgtaatg ttgctaacct ggtcattcac accgacctga actgcctttc
     3841 cgtggttcag tatgcagtgg atgtactaga agttgaacac attattatct gtggccacta
     3901 cggttgcggc ggcgtacaag ccgcagttga aaacccggaa ctggggctta tcaacaactg
     3961 gctgctgcat atccgcgata tctggttcaa acatagctca ttgctcggcg aaatgccgca
     4021 agagcgccgt ctggatacct tgtgtgaact gaacgtcatg gaacaggtgt ataacctagg
     4081 ccactccacc attatgcaat cagcgtggaa acgcgggcag aaagttacca ttcacggctg
     4141 ggcctatggc attcacgacg gcttgctgcg tgatctggat gttactgcca ccaaccgcga
     4201 aacccttgag caacgttacc gtcacgggat ttccaacctc aagctgaaac acgccaacca
     4261 caaataaaaa tgccatgccg gatgcaacac atccggcaac ttcacactta ctcgtccagc
     4321 agaatcactt tgccgatata cggcagatga cggtaacgct gtgcgtaatc aatgccgtaa
     4381 cccaccacaa actcatccgg gatcgagaaa ccgataaatt ctaccgggac gttcacttca
     4441 cgacgggacg gtttatccag cagcgtacaa atcgccagcg acttcggttc gcgcaggctt
     4501 aagatctcac gcactttcga cagtgtattc cccgagtcga tgatatcttc aacaatcagc
     4561 acgtccttgc cacggatatc ttcatccaga tctttgagga ttttcacatc acgggtggtg
     4621 gacatgccgc taccgtagct ggaggcggtc ataaagtcga cttcatgaga tacctgaact
     4681 tcacggcaca ggtccgccat aaacataaat gagccacgca gcagacccac cagcaccata
     4741 tcgctgccgc tgtctttgta acgctcagta atctgacgac ccagttcggc gatacgcgct
     4801 ttaatctccg cttcggggat cattacttct acagtatgtt tcatatctct aaccatatga
     4861 tttaaaaaca aatcacttcg cacgggcagg ttttggcacc gcaacaaagc gcaagacacg
     4921 cagtatacca gtaaaactat tcattcgcgg aatctgttaa taaagctcga attgtgatga
     4981 cgatcacaca tgttaaaacc tataattaat tgctactaaa atattaatga attgaaatgg
     5041 tgtctcttta tggcaattaa caatacaggc tcgcgacgat tactcgtcac gctaacagtc
     5101 ctttttgcag cgctttgcgg gctgtatcta ctcattggcg gaggctggct ggtcgcgatt
     5161 ggcggctcct ggtactaccc tatcgctggc cttgtgatgc tcggcgtcgc ctggatgctg
     5221 tggcgcagta aacgtgccgc gctttggcta tacgccgccc tgctgctcgg caccatgatt
     5281 tggggcgtct gggaagttgg tttcgacttc tgggcgctga ctccgcgcag cgatattctg
     5341 gtcttcttcg gcatctggct gatcctgccg tttgtctggc gtcgcctggt cattcctgcc
     5401 agcggcgcag ttgccgcact ggtggtggca ctgctgatta gcggtagtat cctgacctgg
     5461 gccggattta acgatccgca ggaaatcaac ggcaccttaa gcgccgatgc cacacctacg
     5521 gaagctatct cacccgttgc cgatcaggac tggcctgcct atggtcgcaa tcaggaaggt
     5581 caacgctttt cacctctgaa acaaattaac gccgataacg tccataatct gaaagaagcc
     5641 tgggtgttcc gtactggcga tgtgaagcag ccgaacgatc cgggcgaaat caccaatgaa
     5701 gtgacgccga ttaaagtggg cgacaccctt tacctgtgta ccgctcacca gcgcctgttt
     5761 gcgctcgatg ccgccagcgg caaagagaaa tggcattacg atcctaagct gaaaactaac
     5821 gagtctttcc agcacgtaac ctgccgtggc gtctcttatc atgaagccaa agcagaaacg
     5881 gcttcgccgg aagtgatggc ggattgcccg cgtcgtatca ttcttccggt caatgatggt
     5941 cgactgattg cgattaacgc tgaaaacggc aaactgtgcg aaaccttcgc caataaaggc
     6001 gtgctcaatc tgcaaagcaa tatgccagac accaaaccgg gtctgtatga accgacttcg
     6061 ccaccgatta tcaccgataa aaccatcgtg atggcaggtt cagttaccga taacttctca
     6121 acccgcgaaa cgtctggcgt gatccgtggt tttgatgtca acaccgggga gctgctgtgg
     6181 gcttttgatc ccggcgcgaa agatccgaac gcaatcccgt ctgacgaaca cacctttacc
     6241 tttaactcgc caaactcctg ggcaccagcg gcctatgacg cgaagctgga tctggtctat
     6301 ctgccgatgg gcgtgaccac gccggatatc tggggcggta accgcacacc ggaacaggaa
     6361 cgttatgcca gctcgattct ggcgctgaat gccactaccg ggaaactggc gtggagctac
     6421 cagaccgttc accacgacct gtgggacatg gatcttccgg cacagccgac gctggcggac
     6481 atcaccgtta atggtcagaa agtgccagtt atttacgctc cggcgaaaac cggcaacatt
     6541 tttgtgctcg atcgtcgcaa tggcgaactg gtggttccgg ccccggaaaa accggtgccg
     6601 caaggtgctg cgaaaggcga ttacgttact ccgacccagc cgttctctga actgagcttc
     6661 cgtccgaaga aagatttgag cggtgcggat atgtggggag ccaccatgtt tgaccaactg
     6721 gtgtgccgcg tgatgttcca ccagatgcgc tatgaaggca ttttcacgcc gccatctgaa
     6781 cagggcacgc tggtcttccc gggtaacctg ggaatgtttg aatggggcgg gatttccgtt
     6841 gatccaaatc gtgaagtggc aattgccaac ccaatggcac tgccgtttgt ttcgaaactg
     6901 atcccacgcg gtccgggtaa cccgatggag cagccgaaag atgccaaagg cacgggtacg
     6961 gaatccggca ttcagccaca gtacggtgta ccgtatggtg tcacgctcaa cccgttcctc
     7021 tcaccgtttg gtctgccgtg taaacagcca gcatggggtt atatctcggc gctggatctg
     7081 aaaaccaatg aagtggtgtg gaagaaacgt attggtacgc cgcaggacag tatgccgttc
     7141 ccgatgcctg taccggtgcc gttcaatatg ggtatgccga tgctgggcgg gccaatctcc
     7201 acggcgggta acgtgctgtt tatcgccgct acggcagata actacctgcg cgcttacaac
     7261 atgagcaacg gtgaaaaact gtggcagggt cgtttaccag cgggtggtca ggcgacgcca
     7321 atgacctatg aagtgaatgg taagcagtat gtggtgatct ccgcaggcgg tcacggttca
     7381 tttggtacga agatgggcga ctatattgtg gcttatgccc tgccggatga tgtgaaataa
     7441 ccataatgcc cggagagatc cgggcatatt tccgaatacg gtctttttat accgtaaacc
     7501 ctaacatcat ccccgtatct tcatgctcca gcagatggca gtgcgccata taagcatgtt
     7561 ctttcggtgc gtcgtgatta aacttcacca gcacttcact gacattgcct tctaccttaa
     7621 cggtatcttt ccagcccgcg cgatgagccg ctggcggttt gccattttct gacaagatac
     7681 ggaactgcgt gccgtgaata tggaacggat gcagcatcat gtcgcccacg ccagagataa
     7741 cccaacgttc gtactgccct ttcgccgccg caaacatcgg cttattcata tcaaacgcct
     7801 gaccgttgat tttattggca tggtggaaat cgaacttccc gccgtggttc atatgattca
     7861 tattaccgtg ccccatatgg cccatcatct ggctgtgatc cattccggcc atcgcctgat
     7921 cgccatattt ctccattagc atctgcatac ccatcatatc gagcatcggg tccatagaaa
     7981 gttgcagctt gcgtaccgtc agcccttcca gcgaaggtaa cgcaggcagg ctacttaatg
     8041 tgtctggcaa agcaccggag gcactaatag caatcggctg aatccgcatt accggatgcg
     8101 gcttgtcaaa cggcgcaatc gccatcccca tctggctgac cggtagcgtc accaggtcaa
     8161 agggtttgtt gtcgttaacc tccaccagca cttcaaaacg ctcgcccatc agcaccggca
     8221 gctcgctcac cttcaccggt tcaggtagca gaccaccgtc gctggcaatc acatacagcg
     8281 ggcgattgtc gctggtggcg aaattgagcg agcgggcatt acagccattg agcaaacgca
     8341 ggcgcagcca accacgcggg gcagcgtgtt gtgggtagat tgcaccgttg gtcagcagcg
     8401 tatcgccaaa ccagcccacg gcggcggtca tcacatccag ttgataatca atctgcccat
     8461 cagcgttaaa ttttttatcc tgaacaatca ccggaacatc atcgataccc cactgttttg
     8521 gcagcattaa tttcaggatc tcgtcatctt caattaccac cagcccagcc aaccccatcg
     8581 ccacctgtcg cccagtcttg ccatgttgat gtggatggaa ccagcaagta gcggcaggtt
     8641 gatcaacgtt caacgtcacc gagcgcttgc cacctggcgg aataattccc tgcgggccgc
     8701 cgtcgacttc acccggtact tccagcccgt gccagtgcaa cgtcgtctct tccgtcagtt
     8761 ggttatagat atcaaccgtt accgctttgc cgcgctgtaa tttcaccgcc ggccccagca
     8821 gattgccgtt atagccccag gtagttgcag ttttctcgcc aaaggtagac tgacctgcgc
     8881 caatagttaa ctgaatgcga ttacgggcat cggtcgtgag caaatcaggg atcggtaacg
     8941 ttgggcgttc cgccgcaaag actgcgcggc tccacagcgg caaggctgaa gccacaccca
     9001 gcgcgacgga atattttaag aaatcacgac gttgcatagt tatttcctta ttcttaagca
     9061 ggcgaaacaa aatcaaacgt tgagcatagt ccttcccctt acgggaaggt caagccgcag
     9121 acacaattta atcgacaata atggtcgtcg cctcgctcgc agtgtgctaa cgtttatctt
     9181 ctttaagccc ctggtagaag caatgaagac gtttttcaga acagtgttat tcggcagcct
     9241 gatggccgtt tgcgcaaaca gttacgcgct cagcgagtct gaagccgaag atatggccga
     9301 tttaacggca gtttttgtct ttctgaagaa cgattgtggt taccagaact tacctaacgg
     9361 gcaaattcgt cgcgcactgg tctttttcgc ccagcaaaac cagtgggatc tcagtaatta
     9421 cgacaccttc gacatgaaag ccctcggtga agacagctac cgcgatctca gcggcattgg
     9481 cattcccgtc gctaaaaagt gcaaagcctt ggcccgcgat tccttaagcc tgcttgccta
     9541 cgtcaaataa tccctgatac ctttttgtag aaatattgac cgtgcatccg cggtcaatgt
     9601 tagctattat gttgcgccct ttttttacgg gtgttaacaa aggaggtatc aacccatggc
     9661 cgaaaaaaaa cagtggcatg aaacgctaca cgaccagttt gggcagtact ttgcggtaga
     9721 taacgttctg tatcatgaaa agaccgatca ccaagatctg atcatttttg agaacgctgc
     9781 atttggtcgc gtaatggcgc tggatggcgt agtacaaacc accgagcgcg acgagtttat
     9841 ctatcatgag atgatgaccc atgttccgct actggcccac ggtcacgcga aacatgtgct
     9901 gattatcggc ggcggcgacg gtgccatgct gcgtgaagta acccgacata aaaacgttga
     9961 gtcaatcacg atggtggaaa tcgatgcggg tgtcgtgtcg ttctgccgtc agtatctgcc
    10021 caaccataac gccggtagct acgacgatcc gcgctttaag ctggtgatcg acgatggcgt
    10081 caatttcgtt aatcaaacca gccagacctt tgatgtcatt atctccgact gcaccgatcc
    10141 tatcggtccc ggcgaaagtc tgttcacctc agcattttat gaaggctgca aacgttgcct
    10201 gaatcctagc ggtatcttcg tcgcacaaaa cggcgtctgc tttttacagc aggaagaagc
    10261 catcgacagc catcgcaaac tcagccatta cttcagcgac gttggctttt atcaggcggc
    10321 gatcccgacc tattacggcg gtatcatgac ttttgcatgg gcgacagata acgacgcctt
    10381 acgccatctc tcaaccgaaa ttattcaggc gcgttttctc gcttctggcc tgaaatgccg
    10441 ttattacaat ccggcaatcc atacggcagc ttttgcctta cctcagtatc tgcaagatgc
    10501 actggcttca cagccgtcct aaggagaaga taacaaattg aaaaaactga aactgcatgg
    10561 ctttaataat ctgaccaaaa gtctgagttt ttgtatttac gatatctgct acgccaaaac
    10621 tgccgaagag cgcgacggtt atattgctta tatcgatgaa ctctataatg ccaaccgtct
    10681 gaccgaaatc ctgtcagaaa cctgttccat tatcggggcc aatattctta acatcgcccg
    10741 ccaggattac gaaccacagg gtgccagcgt cactattctg gtgagtgaag aaccggttga
    10801 cccgaaactc atcgacaaaa cagaacaccc cggcccactg ccagaaacgg tcgttgccca
    10861 tctcgataaa agtcatattt gcgtacatac ctacccggaa agccatcctg aaggtggttt
    10921 atgtaccttc cgcgccgata ttgaagtctc tacctgcggc gtgatttctc cgctgaaggc
    10981 gctgaattac ctgatccacc agcttgagtc cgatatcgta accattgatt atcgcgtgcg
    11041 cggttttacc cgcgacatta acggtatgaa gcactttatc gaccatgaga ttaattcgat
    11101 tcagaacttt atgtctgacg atatgaaggc gctgtatgac atggtggatg taaacgtcta
    11161 tcaggaaaat atcttccata ccaagatgtt gcttaaagag ttcgacctta agcactacat
    11221 gttccacacc aaaccggaag atttaaccga cagcgagcgc caggaaatta ccgctgcgct
    11281 gtggaaagaa atgcgcgaga tttattacgg gcgcaatatg ccagctgttt aacggctctg
    11341 gcggagctcc aggctccgcc agatttattt acttctgctg cacgaaattg cggtaagccg
    11401 ccacgacctg cagaaaatcc tcaacgccgc atagcgacag gctttcttcg tcgtagtagt
    11461 tcatcccctc ttccatttca tcgccagcga attccagttg attggcgcga accatcacct
    11521 cttcaccgtc catccacagg gtgtattcat gccctgcccg ttgccaggac cgttcgctac
    11581 ctttcagtgc gtgcgctgct tgttccactt catcaagcaa ggccaggttt tctttcacct
    11641 cttcattaaa ccagtgcccg accacttcat gccccatgga catacgcacc tttaccactc
    11701 cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc
    11761 cgaaatcgca ttgcgccatt gatgtaatta tcgcagcctt accggagaaa aacagcggaa
    11821 cggcgatggt ttagaaataa aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt
    11881 tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg gaaaatcacc ccatcggctt
    11941 tttcggtgta ctgagaaagt tggttgaagt tcagataacg ataagtatca acggcagttt
    12001 tatctacttg cgcaacgtag gtctggtact cttccggcgt cggcagtttg ccaatcagcg
    12061 ccgcaacagc cgccagttct gcagaagcca ggaagacatt cgcgccagtc cccagacggt
    12121 tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt
    12181 tacccataca cagagaacag cccgggatct cgatacgagc cccgctctta ccgaagacgc
    12241 tgtagtagcc ttcttcagtc aactgtgcgg cgtccatacg ggttggcggt gccacccaca
    12301 ggcgggtcgg caactggcct ttatgcgcat ccagcagttt acccgcagca cggaagtgac
    12361 cgatgttagt catgcaagaa ccgataaata cttcgtcgat cttctcgccc tgtaccgcag
    12421 acagcggacg cgcgtcatcc gggtcgttcg gagcacacag gattggctct ttaatatccg
    12481 ccagatcgat gtcaatcact gccgcgtatt ccgcatctgc atcggcttcc agcagctcag
    12541 gattcgccag ccatttttcc atgccctgaa tacgacgttc cagggtacga cgatcgccgt
    12601 aaccttccgc gatcatccac ttcagcagga cgatattaga gttaaggtat tcgatgattg
    12661 gttctttgtt cagcttgata gtacaaccag cggcagaacg ctcagcggac gcatcggtaa
    12721 gctcaaaggc ctgctcaact ttcagatccg gcagaccttc aatttccaga atgcggccag
    12781 agaagatgtt tttcttgcct ttcttctcaa cggtcagcag accttgtttg atggcataca
    12841 gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc
    12901 gcaccagaac ggattccggc atatccagcg gcattacgcc tgttgcggct gcaaatgcca
    12961 ccagaccaga acccgccggg aaagagatac cgatcgggaa acgggtatgg gagtcacctc
    13021 cggtaccgac ggtatccggc agcagcatac ggttcagcca ggagtggatt acgccgtcac
    13081 ccggacgcag cgacacaccg ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt
    13141 tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa agactgcatc accaggtcag
    13201 ccgagaagcc caggcacgcc aggtctttca gttcatcacg ggtcatcggg ccggtagtgt
    13261 cctgtgaacc tacagaagtc attttcggtt cgcagtacgc gcccggacga atgcctttca
    13321 cgccacaggc acggcctacc attttctgcg ccagcgagaa gccgcgatcg ctctcagcga
    13381 catctttcgc ctgacggaac acatcactgt gcggcagacc aagtgcttca cgcgctttgg
    13441 tggtcaggcc acgcccgata atcagcggga tacggccgcc agcacgcact tcatcaatca
    13501 gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt
    13561 cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt caggttagag acgtcgactt
    13621 cgattggcag tgcacccgcg tcttccatcg tgttaaagaa gatgggtgca attttaccgc
    13681 cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg aatatcatcg cccataaacc
    13741 acagaacgga gttcgtggcg gatttacgcg atgaacccgt acccacaacg tcaccgacgt
    13801 acgccagcgg gaaacctttc tgttgcagag cttcaatctg tttgatcggg ccaacaacac
    13861 ctggctggtc tggctcaata ccttcacggg cgtttttcag catcgccagc gcgtgcagtg
    13921 ggatatccgg gcgcgaccac gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc
    13981 cagtgacttt gaagacggta acggtcagtt tttcagccag cgccgggcga ttcagaaacc
    14041 attcggcatc cgcccaggac tgcattacct gcttcgcata ttcgttgcct gctttcgctt
    14101 tctcttctac gtcatagaag ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag
    14161 cgatcggtgc cagtttggca tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac
    14221 cctgcatggt gcccagcagt tcgatggctt tttccggagt cagcagaggg gatttggctt
    14281 cgcctttcgc gatagcagcc aggaagcctg ctttgacata ggcggcttca tcgacgccag
    14341 ggggaacacg gttggttaac agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt
    14401 tcagcagctc tacaagtgcg gccatttggt ttgcatccag gggtttgggc gcaatcccct
    14461 cagcggcacg ctcagctacg tgcttacggt attcttctag cacgacggtt ctcctcgctc
    14521 tcattgtcat agtgcggcag gcgattctct tcacgctcct gtgagacagc agtttgtagg
    14581 gtaaatgccc agtaccgcaa cgggcagaat agcaggattt tgacgacgtg ttaatctgtt
    14641 tacaaaaaag caacataaaa aagtggctga atcgttaagg atggtctaga gattgtttcc
    14701 ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc tttctcaacg aatcccaaga
    14761 ttagtcaaaa tttaaactac cgcctcttta tactcggatt aacagcacct gcgggtggca
    14821 gttcgccgac cattgcgatt tccttgagat ccgaattatg aaaatgactt tgccgtttaa
    14881 accccatgtg ctggcactaa tttgcagtgc cgggctttgt gccgcctctg ccgggctata
    14941 tataaaaagc cgcacagtgg aagcggctgt ggaaacgcaa tcgacacaac tggctgtgtc
    15001 tgacgctgcc gcggttacgc ttcctgcaac ggtttccgcg cctcccgtaa cacccgccgt
    15061 cgttaaatcc gcattcagca ctgcacaaat agatcaatgg gtcgcgcccg tcgcgctgta
    15121 tcccgacgcc ctactttcgc aggtgctgat ggcatcaacc tatccggcaa acgttgctca
    15181 agcagtgcaa tggtcgcacg ataatccact taaacaaggc gatgctgcta ttcaggcggt
    15241 atctgaccag ccgtgggacg ccagcgttaa atcactggtg gcctttccac aattgatggc
    15301 attgatgggc gaaaacccgc aatgggtgca aaacctgggc gatgcttttc tggcgcagcc
    15361 gcaggacgtg atggactcgg tacagcgatt gcggcaactg gcgcaacaaa ccggttcgct
    15421 gaagtcatca aacgaacaaa aagttattac cacaacgaag aaagctgtac cggtaaaaca
    15481 gacagtcacg gcacccgtca taccatccaa taccgtttta actgccagcc ccgtcattac
    15541 agagcctgca acaaccgtca tttccattga gcccgccaat cctgatgtgg tctatattcc
    15601 caactacaac ccaaccgtgg tttacgggaa ctgggccaat actgcgtatc cgccggttta
    15661 tctgccacca ccagccggag aaccgtttat tgacagcttt gtgcgcggat tcggctatag
    15721 catgggtgtt gctaccacgt acgcactatt cagcagcatc gactgggatg acgacgatca
    15781 tgaccatcat catcatgacg atgatgatta tcatcaccac gatggcggtc atcgtgacgg
    15841 taatggctgg caacacaacg gcgacaacat caatatcgac gtcaacaatt tcaaccgtat
    15901 caccggtgag catcttactg ataagaatat ggcatggcgg cacaatccaa actaccgtaa
    15961 tggtgtgccc tatcatgatc aggatatggc aaagcggttt catcaaaccg atgtcaacgg
    16021 cggaatgagt gccacgcagc tacctgctcc aacacgcgac agccagcgtc aggcggcagc
    16081 aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt acacgagata cccaacgtca
    16141 ggcagcggca cagcggttta atgaagctga acactatggg agctatgacg acttccgcga
    16201 cttcagccgt cgccaacccc tgacccagca acaaaaggac gccgctcgtc agcgttatca
    16261 gtcagcttct cctgagcagc gccaggcagt tcgcgagaga atgcagacta acccgcagat
    16321 ccagcagcga agagaggcag cgcgtgagcg cattcagccc gcctcgcctg agcagcgcca
    16381 ggcagtccgc gagaaaatgc agactaaccc acagatccag cagcgaagag acgcagcgcg
    16441 tgagcgtatt cagtcagcct cgcctgagca gcgccaggtg tttaaggaaa aagtacagca
    16501 gcgcccactg aaccaacagc aacgtgataa cgcccgccag cgtgttcaat cagcatcacc
    16561 tgaacaacgt caggtttttc gggagagagt tcaggagagc cgcccacaac gtctaaacga
    16621 cagtaaccgt actgccagat tgaataacga tcaacggtca gcagtacgcg aacgtctctc
    16681 tgagcgcgga gcaaggcgac tggaaaggta aattacagac gtaaaaaaag cggcgtggtt
    16741 agccgctttt ttaattgccg gatgttccgg caaacgaaaa attacttctt cttcgctttc
    16801 gggttcggca ggtcggtaat gctaccttcg aacacttctg ccgccaggcc cacagactcg
    16861 tgcagagtcg ggtgcgcatg gatggtcagc gcgatgtctt cagcgtcaca acccatttcg
    16921 attgccaggc cgatttcacc cagcagctcg ccgccgttgg taccgacaat cgcaccacca
    16981 atcacacggt gagattcttt gtcgaaaatc agcttggtca taccgtctgc gcagtcggaa
    17041 gcgatagcac gaccagaagc agcccacggg aaggtggcgg tttcatagct gatgcctttc
    17101 tctttcgctt ctttctcagt cagacctacc catgcaactt ctggttcggt ataggcgata
    17161 gacgggataa ctttcggatc gaagtagtgt tttttaccgg cgataacttc agcggcaacg
    17221 tgaccttcgt gaacaccttt gtgtgccagc atcggctgac caacgatatc gccgatagca
    17281 aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtcc
    17341 acttccacgc cagctttgcc tgcgtcgagg tttttaccgt tcggcacacg accaatcgct
    17401 accagcacgg cgtcgtaacg ctgcggttca gcgggtgctt ttttgccttc catcgtcaca
    17461 taaataccgt cttctttcgc ttcaacggcg gtaactttgg tttccagcat caggttgaat
    17521 ttcttgctga tacgcttggt gaagacttta acgatgtctt tgtcagctgc cgggataacc
    17581 tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg cgtggtatac ggtgcccatt
    17641 tccagaccga tgataccgcc acccattacc agcaggcgtt ctggtacttc tttcagttcc
    17701 agcgcgtcag tggagtccca gatacgcgga tcttcatgcg gaataaacgg cagttggatc
    17761 gggcgagaac ccgctgcaat gatcgcgttg tcgaagttga tcacggtttt accgttctca
    17821 ccttcaactt ccagggtgtt agccccggta aatttaccca gaccgttgac cactttgact
    17881 ttgcggcctt tcgccatacc agccagacca ccggtcagct gattgatcac tttctctttc
    17941 caggtacgaa tcttgtcaat atcggttttc ggttcgccga agacgatacc gtgttcagcc
    18001 agcgctttgg cttcttcgat aacttttgct acgtgcagca gtgctttaga agggatacag
    18061 ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt ctacgattac ggtttccaga
    18121 cctaaatcag cgcaacggaa ggcagcggag taacctgcgg ggcctgcccc aagtaccacg
    18181 acctgagttt tgatttcagt actcatcatg acctctatat atttatctcc ggcggtcata
    18241 cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc ctcaccccag tcacttactt
    18301 atgtaagccc ctggggattc tggcgcttgc taccttgctg cacccagaaa tccatagggt
    18361 atgtgaagta ataccctaac caccaccggg tcgttctatc cgtcggtatt ttacaaaatt
    18421 gttaacaatt tttaaacaac aaacggcaac cgatttgtct attcgctaat aacctcaata
    18481 cattcatgag attaccagaa aaaagccggc cgttgggccg gctcttttac ttacatcacc
    18541 agacggcgaa tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc accatcagca
    18601 ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca gcatcagacg cggcacgaac
    18661 tctttaccat tccacaccgg ctccatcgcg gacttggaaa cgccgaggat agccacttcc
    18721 ggcgcgttca caatcggcgc gaagtgggta gtacccaggc cgccgatgct ggagatggtg
    18781 aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt cttagaaata
    18841 gtcatcagct cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt gaataccgga
    18901 acaaccagac cgttcggggt atccaccgcc acaccgatgt tgatgtattt cttcagggtc
    18961 agacgctgac cgtcttccga cagcgaacta ttgaagcgag gcatctgctc aagagctgca
    19021 gcaacggctt tcatgatgaa gacaaccggg gtgatcttca catccagctt acgtttcgcc
    19081 gcttcttcgt tctgctgttt acggaacgct tccagctcgg tgatatcggt tttgtcgaag
    19141 tgggtaacat gcgggatcat cacccagtta cggctcaggt tagcaccaga aattttctgg
    19201 atacggccca gttccacttc ttcgatttca ccaaacttgc tgaagtccac cttcggccac
    19261 ggcagcatgc ctgggatacc gccgccagtc gccgccggag ctgcttctgc acgtttgata
    19321 gcttctttca cgtaagcctg aacgtcttcg cgcaggatac gacctttacg gccagtgccc
    19381 ttcactttcg ccaggttaac accaaactcg cgtgccagac ggcggatcag cggagtcgcg
    19441 tgaacataag cgtcgttttc agcaaattca gatttgcctt ccgctttcgc agctggtgct
    19501 gctgccgggg cttcagcttt tgctgccggt gccggcgctg ccgcttcctg tttcgcagga
    19561 gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa tcagcgagcc agttttcact
    19621 ttatcgccaa cgttgacttt cagttccttc acgacgcctg caaacggcgc cggaacttcc
    19681 atagaagctt tgtcgccttc tacggtgatc agtgactgtt cagcggcaac tttgtcgccc
    19741 actttcacca tcacttcagt cacttcaact tcgtcaccgc cgatatccgg aacgttaact
    19801 tctttcacgc cagctgctgg tgcaggggcc gctgccggag ccgcttcctg tttagccgcc
    19861 ggagctgccg cgcctgcttc acccgcgact tcgaagacca taatcagcga gccggtagac
    19921 actttgtcac ccacgttcac tttgatctct ttcacggtgc cagcaaacgg agccggaact
    19981 tccatagaag ctttatcgcc ttctacggtg atcagcgact gttcagcttc aactttatcg
    20041 ccaactttca ccaggatttc ggtcacttca acttcgtcgc tgccgatatc cggaacgtta
    20101 acgtcttttg ccgccgcagc cgcgggtgct gctgccggag ctgcttcttt cttctcttct
    20161 gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg
    20221 ccggtctggg ttttatcgcc aacagagact ttgatctctt taacgatacc cgcctgcgga
    20281 gacggaactt ccatagaggc tttgtcgcct tctacggtga tcagcgactg ttcggcttca
    20341 actttgtcgc ccactttgac caggatctcg gtgatttcaa cttcatcagc cccgatgtcc
    20401 ggtactttga tttcgatagc cattattctt ttacctctta cgccagacgc gggttaactt
    20461 tatctgcatc gatgttgaat ttggcgattg cgtcagcaac cactttctta tcgatttcgc
    20521 cacgtttagc cagttcgccc agcgccgcaa ccacgacata agaagcatca acttcgaagt
    20581 ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc agtacgcggt
    20641 agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa cagtttcata tagtcggtag
    20701 atgccactgc cggagcgtcg ttcatcacct gagcgatata cggaacgcgc ggagtttcca
    20761 gcggatgaag catgttccag cgttcacaat cctgaccatc acgcgccagc tcggtgaagg
    20821 aggtcacgct ataaacgtca gaacctacgc cgtaatcttt cgccaggatc tctgccgctt
    20881 cacggacgtg acgcaggata gaaccggagc ccagcagctg aactttacct ttgctacctt
    20941 caatggtttc gagtttgtag atacctttac ggataccttc ctcagcacct tccggcattg
    21001 ccggcatgtg gtagttttcg ttcagcgtgg tgatgtagta gtaaacgttc tcttgttttt
    21061 caccgtacat acgctccaga ccgtcatgca taatgacagc aacttcgtaa gcgtaagccg
    21121 ggtcgtaaga gatacagttc ggaatagtca gcgactgaat gtggctgtga ccatcttcat
    21181 gctgcagacc ttctccgttc agggtggtac gaccggaagt accgccgatc aggaagccac
    21241 gcgcttgctg gtcgccagcc gcccagcaca gatcgccaat acgctggaag ccgaacatcg
    21301 agtaatagat gtagaacggg atcatcggca gattgttggt gctgtaagag gtcgccgctg
    21361 ccagccagga acaacctgcg cccagctcgt tgatcccttc ctgcagaatc tgacctttct
    21421 cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg
    21481 ggctgtaaat accaatctga cggaacagac cttccatacc gaaagtacgc gcttcgtcgg
    21541 cgatgatcgg taccagacga tctttgatcg acttgttctt cagcattacg ttcagggcac
    21601 gaacgaaagc gatagtggta gagatctctt tgctctgctc ttccagcagc gcgccgaagt
    21661 cttgcaggct cggcagctca agcttctcgg tgaagttcgg ctgacggctt ggcagataac
    21721 cgtgcagttt ctgacgctgt gcgtgcagat aggtatgctc ttcagaacct tccgggaagg
    21781 tgatgtacgg cagtttttcg atatctgcat cagacaccgg cacattgaaa cggtcgcgga
    21841 tgtgacgcac gccgtccatg ttcattttct taacctggtg cgcgatgttt ttaccttcag
    21901 ccgcgtcgcc catgccgtaa cctttaatgg aatgagcaag gattactgtc gctttgcctt
    21961 tggtttcctg cgctttcttg aatgcagcgt agattttctt cggatcgtga ccaccacggt
    22021 tcagtgccca gatctgctcg tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt
    22081 taccgaagaa gtgttcacga acgtacgcac catctttcga tttgaaggtc tggtagtcgc
    22141 cgtcaacggt ttcgttcatc agctggatca gtttaccgct ggtatcttta cgcagcagtt
    22201 catcccaacg gctaccccac atcactttga tcacgttcca gccagcacct tcgaagatgc
    22261 cttccagttc gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt
    22321 tacagttgat aacgaagacc aggttatcca gtttttcacg ggtagcgatg gtgatcgcac
    22381 ctttggattc cggttcgtcc atttcaccgt cgccgaggaa cgcgtaaacg gtctgtttag
    22441 aggtatcttt caggccacgg tgttccagat atttcaggaa tttagcctgg taaatagcac
    22501 caatcggccc cagccccata gatacggtcg ggaactgcca gaattccggc atcagtttcg
    22561 ggtgcggata ggaagagagg ccattgccgt gaacttcctg acggaagtta tccaactgct
    22621 cctgagtcag acgaccttcc aggaaagcac gagcgtacac gcccggggag atgtggccct
    22681 ggaagtaaac caggtcgccg ccatcctgct cgttgcgtgc acggaagaag tggttaaagc
    22741 acacatcata aatggttgcg gaagactgga aggacgccat atggccgccc agttcgaggt
    22801 cttttttcga cgcacgcagc accgtcatga tggcgttcca gcggatagct gaacgaatac
    22861 ggcgttccag ttccagatta cccggatact ccggttgttc ttcaacgggg atggtgttga
    22921 tgtagttgct gatacctgtg cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa
    22981 gcagttggtc gatcagatac tgagcacgct caacaccttc ttcacggatg accgattcga
    23041 tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa cgttctgaca
    23101 tgggttattc cttatctatc taataacgtt gagttttctg gaacctgtcc cattgaactc
    23161 tcgccggaaa gctcaataag acaggttcta cgtttagttg ccgcgctttt atatgcgctt
    23221 gatttacaac atcttctgga taatttttac cagtaaaatc actaattctt tcgttgctcc
    23281 agacgacgca gagaacgctc acggcggctc tcttcacgac ttctgtcgag caaaatttct
    23341 tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt cttccggctt accggccata
    23401 atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa
    23461 tagagcaatt cgaagttctg gcggacattc tgggccaaca tcggctccat acaccttagc
    23521 agatgaagca gaaccacatt gtgggccgct tcagtgacag caatctgata ctggagtacg
    23581 gcgtttgatt ccgcgtccag atcgccagac tgctgcgcca gttctatggc gtgatggagt
    23641 tcacggatgc gttccttgtc ttcatcggta ctacgcagcg cggcgtaata agcggcgata
    23701 ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg actcaggatg gtcggagagc
    23761 agctccacca gcggatcgct gaagctttgc cataggctgc tctggacaaa agtgccgcca
    23821 ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag
    23881 ggacgggaga cgtcaaactg ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg
    23941 cggagagtgc cttcgaggat caaaaactcc agttgctgct caatcacatc ggagagtttt
    24001 ggttggcgga ttttgctgta ggccatgagt tcctgtctta agccacttgc cgaagtcaat
    24061 tggtcttacc aatttcatgt ctgtgacgct aaagtaacaa agtattcacc ttatgtccat
    24121 acaggttttg attgaaatca tgaaactgtg cacattttaa caacttgaca tatataacgt
    24181 ttcaaagttg taactatgca caaatgtaga ctttacgtag gaaagggatt ttttaacgat
    24241 tattaaacta taaaaatccg aattgaaccg attcacttac caattttgtg atttttaatt
    24301 caattaaaac gaatttaaat tcattctaca tattgagagg ggttgaggct gagctttaca
    24361 aacggtttct ttttaagcaa ctcatcttca accatgcata aagcgggtgc attcgctgcc
    24421 gcataccatt attcttgatc tgacggaagt ctttttgtaa caattcaaac ttctttgatg
    24481 taaacaaatt aatacaacaa acggaattgc aaacttacac acgcatcact gcgtagatca
    24541 aaaaaacaac caccgcacga ggtttcatga tggaaggtca acagcacggc gagcagctaa
    24601 agcgcggcct taaaaaccgc catattcagc ttatcgcgct gggtggcgcg atagggaccg
    24661 ggttattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt
    24721 acgccattgc tggttttatc gcctttctga tcatgcgtca gctgggtgaa atggtggtcg
    24781 aagaacctgt cgcaggctcc tttagccact ttgcttataa atactggggc agttttgccg
    24841 gtttcgcctc tggctggaac tactgggtac tgtacgtttt agttgccatg gctgagctga
    24901 ctgccgtggg taaatacatt cagttctggt atccggaaat ccccacctgg gtttctgccg
    24961 ccgtattctt tgtggtgatt aacgccatca acctgaccaa cgttaaagtg tttggcgaga
    25021 tggagttctg gtttgccatt atcaaagtta tcgctgtggt agcgatgatc atcttcggcg
    25081 gctggctgct attcagtggc aacagcggcc cgcaggcgac cgttagcaac ctgtgggatc
    25141 agggtggttt cctgccgcac ggcttcaccg ggctggtgat gatgatggcg attatcatgt
    25201 tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc agaagctgat aacccggagc
    25261 aaagtatacc gaaagcaact aaccaggtta tctaccgcat cctgattttc tatattggtt
    25321 cgttagccgt tctgctctca ctgatgccgt ggacccgcgt taccgccgat accagtccgt
    25381 ttgtgctgat cttccacgag ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg
    25441 tactgactgc ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt
    25501 ttggtctggc acaacagggt aatgcgccaa aagcgctggc gtctgtcgat aaacgtggtg
    25561 taccagtaaa taccattctg gtgtctgcac tggtaacggc gttgtgcgta ctgattaact
    25621 accttgcccc agagtccgct ttcggactgt taatggcgct ggtggtatct gcactggtaa
    25681 tcaactgggc gatgattagc ctggcgcata tgaaattccg tcgcgccaag caggaacaag
    25741 gcgtggtaac tcgcttccct gctctgcttt atccgctggg taactggatc tgcctgctgt
    25801 ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc
    25861 tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca
    25921 aagccgtaaa agcgcattaa tctctctacg ccctcacccg tacagggtga gggcaataat
    25981 ctttacacca acgccccgta aatcgtcagt agtgcaatca ccaccacgac cacgaacgag
    26041 gttttcttcg ccattgaaac cgctgccttc ggcgtctcca ccttatcgac atgcggttca
    26101 cgcgccagag agaactgcgc cagacgcgtt aacacctgat actgcgaagt atggaagtca
    26161 cccagcgaag caaaccaggc cggtaacgct ttctccccat gaccgatcaa ggcatatacc
    26221 acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacggcatc aatgccggac
    26281 tgtaaacgat gatgcggcgt ctggtatcgc gccagccagt attgccatgc acgcaaaaac
    26341 gcatacccca tcagcgtaac gggtccccag attcccccca caatcaacca gaacagcgga
    26401 gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca
    26461 cgttcatcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtgaca
    26521 cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact
    26581 ttacctgcgc caatacacag caaaccaatc agcagccaca ccagcagcgt gggaacgttg
    26641 aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc
    26701 atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga
    26761 tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag
    26821 gttgtaaata gcgtcatgtt gtctccttgc tgaccagcgc acgaaaccgc gcccaatcaa
    26881 atgcagggcc gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgttat
    26941 tagcgatatc tggatagcga tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact
    27001 gcgcatcggt atacgccagc gtatcggtgc cttcaagctc aatcccaata gaaaaatcat
    27061 tgcagcgttc gcgcccctga tactgagaga ctcccgcatg ccatgcacgt ttatcgaaag
    27121 gaacatactg gactatttca ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat
    27181 gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat agttccagtg aataatgcgt
    27241 cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg
    27301 tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa
    27361 ccagccaccc ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt
    27421 tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc
    27481 tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat
    27541 ccccggcgcg gtggcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa
    27601 tgacattacg gcaaaacttt taccggaaaa ctctcgctct catgccacgg tgatcacccg
    27661 cgagaatggc gtattttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg
    27721 cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaacg ccaatcaacc
    27781 cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa
    27841 ttttgtgcaa accctttcag gagttgccag taaggtacgc cactatgtcg aattactgga
    27901 aggcaccaac acgcagttgt tggatacgcg caaaacctta cctggcctgc gttcagctct
    27961 gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt ctggggcttt ctaatgcctt
    28021 cctgatcaaa gaaaatcata ttattgcctc cggctcagtg cgccaggccg tcgaaaaagc
    28081 gtcctggctg cacccggatg cgccagtaga agtcgaagta gagaatctgg aagaacttga
    28141 tgaagccctg aaagcaggag ccgatatcat catgctggat aacttcgaaa cagaacagat
    28201 gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac
    28261 tgacaaaaca ctgcgcgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct
    28321 aactaaacac gtacaagcac tcgacctttc aatgcgtttt cgctaattgc tgacagattt
    28381 atccgctcca tcgcgggcgg ataatctccc cttccccaac tttcttcgta acgcctcgca
    28441 aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg aagctgcttt cccgtttcgt
    28501 tttttgcccc tcgcttgtgc tgatggcgtt tgccaaagta gcgccaacca aatcaaggag
    28561 cgaaacagat ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca
    28621 tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagctg
    28681 cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc
    28741 tggaacatgg tg
//
LOCUS       DADLTD010000027        28491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLTD010000027 DADLTD010000000
VERSION     DADLTD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.027"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(58..753)
                     /gene="aqpZ"
                     /locus_tag="JN701_000974"
     CDS             complement(58..753)
                     /gene="aqpZ"
                     /locus_tag="JN701_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY7363498.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1179..2837
                     /locus_tag="JN701_000975"
     CDS             1179..2837
                     /locus_tag="JN701_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY7363499.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2834..3790)
                     /locus_tag="JN701_000976"
     CDS             complement(2834..3790)
                     /locus_tag="JN701_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY7363500.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3941..5056
                     /gene="macA"
                     /locus_tag="JN701_000977"
     CDS             3941..5056
                     /gene="macA"
                     /locus_tag="JN701_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY7363501.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5053..6999
                     /gene="macB"
                     /locus_tag="JN701_000978"
     CDS             5053..6999
                     /gene="macB"
                     /locus_tag="JN701_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY7363502.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(7072..7296)
                     /gene="cspD"
                     /locus_tag="JN701_000979"
     CDS             complement(7072..7296)
                     /gene="cspD"
                     /locus_tag="JN701_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY7363503.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7619..7939
                     /gene="clpS"
                     /locus_tag="JN701_000980"
     CDS             7619..7939
                     /gene="clpS"
                     /locus_tag="JN701_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY7363504.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7970..10246
                     /gene="clpA"
                     /locus_tag="JN701_000981"
     CDS             7970..10246
                     /gene="clpA"
                     /locus_tag="JN701_000981"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY7363505.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10590..10677)
                     /locus_tag="JN701_000982"
     tRNA            complement(10590..10677)
                     /locus_tag="JN701_000982"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10641..10643),aa:Ser,seq:gga)
     gene            complement(10931..11149)
                     /gene="infA"
                     /locus_tag="JN701_000983"
     CDS             complement(10931..11149)
                     /gene="infA"
                     /locus_tag="JN701_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY7363506.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11434..12138)
                     /gene="aat"
                     /locus_tag="JN701_000984"
     CDS             complement(11434..12138)
                     /gene="aat"
                     /locus_tag="JN701_000984"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY7363507.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12180..13901)
                     /gene="cydC"
                     /locus_tag="JN701_000985"
     CDS             complement(12180..13901)
                     /gene="cydC"
                     /locus_tag="JN701_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY7363508.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13902..15668)
                     /gene="cydD"
                     /locus_tag="JN701_000986"
     CDS             complement(13902..15668)
                     /gene="cydD"
                     /locus_tag="JN701_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY7363509.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15791..16756)
                     /gene="trxB"
                     /locus_tag="JN701_000987"
     CDS             complement(15791..16756)
                     /gene="trxB"
                     /locus_tag="JN701_000987"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY7363510.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17301..17795
                     /gene="lrp"
                     /locus_tag="JN701_000988"
     CDS             17301..17795
                     /gene="lrp"
                     /locus_tag="JN701_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY7363511.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17930..21958
                     /locus_tag="JN701_000989"
     CDS             17930..21958
                     /locus_tag="JN701_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7363512.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            22113..22724
                     /gene="lolA"
                     /locus_tag="JN701_000990"
     CDS             22113..22724
                     /gene="lolA"
                     /locus_tag="JN701_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY7363513.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPSQGVTVDDQRK"
     gene            22735..24078
                     /locus_tag="JN701_000991"
     CDS             22735..24078
                     /locus_tag="JN701_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY7363514.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24169..25461
                     /gene="serS"
                     /locus_tag="JN701_000992"
     CDS             24169..25461
                     /gene="serS"
                     /locus_tag="JN701_000992"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY7363515.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25700..28144
                     /gene="dmsA"
                     /locus_tag="JN701_000993"
                     /pseudo
     CDS             25700..28144
                     /gene="dmsA"
                     /locus_tag="JN701_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            28155..>28491
                     /locus_tag="JN701_000994"
     CDS             28155..>28491
                     /locus_tag="JN701_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="oxidoreductase, Fe-S subunit; terminal electron
                     transfer protein for the reduction of DMSO; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY7363516.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYG"
BASE COUNT         7187 a         7054 c         7624 g         6626 t
ORIGIN      
        1 caacatataa caacatctta aaaaaagcct gacattacgc caggccttct gcgttaatta
       61 atcacgcttt tccagcaggg tccggtaaat cagaccaccg ataatgccgc cgacaattgg
      121 caccacccag aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc
      181 ggtgctgcgc gccgggttaa cagaagtgtt agtcaccgga atactaatta agtgaatcag
      241 ggttaaggcc agaccaatag cgatcggcgc aaaacctgcc ggcgcgaatt tggcggttgc
      301 gccgtggatc accaacagga aacctgcact caataccagt tcaactacca gcgcggaaag
      361 catggaataa ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc
      421 cgcgtcaaaa cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc
      481 gccgacaacc tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc
      541 ccataaacca atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcaaa
      601 ggccatcgtc agaacggtca gaccgaacgc caacgccacg ccggcaaaac caatgcctaa
      661 ttccgggaag cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt
      721 accaaaacat tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccctg
      781 tgaaaaatat ggtcgtttta tagggtcgtc gtaaaaaaat gacgacggaa ataatgcgcg
      841 gctattttaa aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt
      901 agggcaacag cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcacgcaa
      961 aagatttaat cctttaggcg taataaaaaa taatatatca tgctaattat ttgattttgt
     1021 tgtttttgca gacttatcag caagagggag tataacgcga ttattcgctc atttttcaga
     1081 catttgccat gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg
     1141 ccagcgtaaa ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa
     1201 ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct ggaacaaaac aacgtcctga
     1261 ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc
     1321 cagaatcaga tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca
     1381 acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac
     1441 atcgggttcg ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc
     1501 accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc
     1561 gcagttttct cgataaagac ggacatccga ttgatgtcga ggatattaac gatcaggcac
     1621 gccatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta
     1681 ttcgtaacgg cacggtgcca aatgtcccta atgtggaagt caccgcgcgc cagctcgatt
     1741 tcctcgcccg tgagttatcc tcacatccgc aaaatctctc tgatgggcag attcgtcagg
     1801 gactttccgc aatggtacag ctgcttgagc attatttctc tgagcagggg gccggacagg
     1861 cgcgatatcg tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg
     1921 acatcatcaa ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg
     1981 gcctgtttgc tactttattg caggcaaaag gcacattgag actggataaa gacgcgcgtc
     2041 cattgttgct gatcgaagat ccagaaaccc gtttacaccc cattatgctt tcagttgcct
     2101 ggcatctgtt gaatcttctg ccattgcagc gcattgccac caccaactcg ggtgagttgc
     2161 tttcgttaac gccggtagag catgtttgcc gactggtacg tgagtcctcg cgcgttgccg
     2221 cctggcgtct ggggccgagt ggcttgagta ccgaagatag ccgacgcata tcctttcaca
     2281 ttcgttttaa ccgtccgtca tcgctgtttg cacgctgctg gttgctggtg gaaggggaaa
     2341 cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag
     2401 ggatcaaggt cattgagttt gcccagtccg ggctaaagcc gctggttaaa tttgcccgcc
     2461 gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg
     2521 ctacggtacg cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt
     2581 taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc
     2641 gcgtggcgca aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga
     2701 tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatagaggca ggacgtcgtg
     2761 gtgtggactc cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc
     2821 gcggtcgcgc ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca
     2881 tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc
     2941 gtgctatctg tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgatt
     3001 cccagaaagc gttgtaatca gcgtggatct tgccttcttt atcgcgataa cgcaggctgc
     3061 ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa
     3121 acagacaggc cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg
     3181 cattctggat ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca
     3241 tcgttctttt cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt
     3301 cgctgttgcg aaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca
     3361 gcttaagcgt gaactgctcg ccgtttttgc cttccagctt cgccagttgc agcccggggg
     3421 tattcaaata taagctgaat tcttccgccg acatacatcc gcggagtaac gcataatggt
     3481 aacgtaacgc ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgca
     3541 gacgcacagg cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa
     3601 gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct
     3661 ggcgaaaact acggcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa
     3721 gaaaaagtga caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg
     3781 acatattcat gaaatcattg gttatacatg acgtcgattt caccattgcg tatcttaacc
     3841 aaacatcaat agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag
     3901 ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt
     3961 gaagaagcgt tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag
     4021 aattcttaac gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca
     4081 gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgatg tgggcgcgca
     4141 ggtcagcggt cagttgaaaa ctctgtcggt ggcgattggc gataaagtaa aaaaagacca
     4201 gcttttaggg gttattgatc ctgaacaggc tgaaaaccag attaaggagg tcgaagcaac
     4261 gctgatggag ctacgtgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt
     4321 gacgtattcc cgtcagcaac gtctggcaca aacgcaggcg gtttcacagc aggatctcga
     4381 caccgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcggaaat
     4441 caagcgcaat caggcttctc tcgatacggc taaaaccaat ctcgattaca ctcgcatcgt
     4501 tgccccgatg gccggggaag tcacgcaaat caccactctg caaggccaga cggtgattgc
     4561 cgcacaacaa gcaccgaaca ttctgacgct ggcagatatg agcaccatgc tggtaaaagc
     4621 gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagctt ggtttacggt
     4681 gcttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtactac cgacgccgga
     4741 aaaggttaac gacgctattt tctattacgc ccgttttgaa gtccccaacc ccaatggttt
     4801 gctgcggctg gatatgactg cgcaagtgca tattcagctc accgatgtga aaaatgtgct
     4861 gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt
     4921 gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga
     4981 tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc
     5041 aggagctgca caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc
     5101 ggtgatgagc aggttgaggt gctgaagggc atcaccctcg atatttatgc cggtgagatg
     5161 gtagcgattg ttggcgcttc aggttccggt aaatcgaccc tgatgaatat tctcggctgt
     5221 ctggataagg ccaccagcgg cacctatcgc gtcgccggtc aggatgttgc cacactggac
     5281 gccgatgcgc tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat
     5341 ttactttcgc atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt
     5401 gagcggaaac agcggctgct tcgcgcccag gagttactgc aacgactggg gctggaagac
     5461 cgtacagagt attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg
     5521 cgggcattga tgaacggcgg tcaggtaatt cttgccgatg aaccaaccgg cgcactggac
     5581 agccattctg gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatatg
     5641 gtgattatcg tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt
     5701 cgcgacggcg aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg
     5761 acggagcctg ttgtcaacac ggtgtctggc tggcggcagt ttgtcagcgg ttttaacgag
     5821 gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg
     5881 ctggggatta ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc
     5941 aaacaaatgg tgctggcgga tattcgttct attggtacga atactattga tgtctatccc
     6001 gggaaagatt ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta
     6061 atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg
     6121 cgcctgcgtt ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat
     6181 tttaatgtct atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac
     6241 ggtcgtgcgc aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa
     6301 gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg
     6361 gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac
     6421 agcacgatgt ccgggcgagt tatgggccag tcgtggctga actccattac tgtcagggta
     6481 aaagaaggat ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc
     6541 cacggaaaga aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag
     6601 accacacgta ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc
     6661 ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc
     6721 attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc
     6781 gtactggttt gcttggtcgg tggcgcgttg ggaataacgc tgtcactgtt aattgctttc
     6841 accttgcagc ttttcttacc cggctgggag attggttttt caccgttggc actgctgctg
     6901 gcgtttctct gctcgacggc caccgggatt ttatttggat ggttacccgc acgaaatgcg
     6961 gcacgactgg atccagtaga tgctctggta cgagagtaat ttttgagata aaaatgccag
     7021 ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac
     7081 tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg
     7141 gacatcaaac tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt
     7201 ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt
     7261 ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt
     7321 atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact
     7381 ctagaaatac aatttagaga attagggcga gccgtcaagc gtttgacagg ggccaggggg
     7441 caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg
     7501 ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa
     7561 gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat
     7621 gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc
     7681 gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga
     7741 gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt
     7801 gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc
     7861 agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg
     7921 tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca
     7981 agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt
     8041 tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct
     8101 ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca
     8161 aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt
     8221 tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggtaac
     8281 cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg catatctgtt
     8341 gcgtaaacat gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa
     8401 agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg
     8461 tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc gcgtgggcgg
     8521 aatcgaccca ctgattggtc gtgagaagga gctggagcgt gctattcagg ttctctgccg
     8581 tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc
     8641 ggaaggtctt gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac
     8701 gatttactct ctcgatatcg gttctctgtt agcgggcact aaatatcgcg gcgactttga
     8761 aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat
     8821 tgatgagatc cacaccatta tcggtgcggg tgcagcgtct ggtggccagg tcgatgcggc
     8881 taacctgatc aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta
     8941 tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat
     9001 tgatattact gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa
     9061 gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcag cggtagagct
     9121 ggcggtgaaa tacattaacg atcgtcatct gccggataaa gccattgacg ttatcgacga
     9181 agcgggcgct cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc
     9241 ggatattgag tccgtggtgg cccgtattgc gcgcattcca gagaagagtg tttctcagag
     9301 tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggtcagga
     9361 taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga
     9421 acataaaccg gttggttcgt tcctgtttgc cggccctacc ggggtcggga aaacagaggt
     9481 gacggtacag ctttcgaaag cgttgggcat tgagctgctg cgctttgata tgtccgagta
     9541 tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga
     9601 tcagggaggt ttgctgactg atgcggtcat caagcatcca catgcggtgc tgttgctgga
     9661 cgaaatcgag aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg
     9721 tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac
     9781 caccaacgcc ggggtacgtg aaactgagcg taaatccatt ggtcttatcc accaggataa
     9841 cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct
     9901 cgacaacatt atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa
     9961 attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca
    10021 ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat
    10081 ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc
    10141 gctggtggac ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg agctgactta
    10201 cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgttaggt
    10261 aggttggtca agtccgtaat ctcgaaagag gttacggact ttttgtttat ggggtggagg
    10321 aggttcagac ccttttttta atgatgatgg taagttgttg ataattagtg ctgcgggaag
    10381 gtaaggataa aaaagggtgc tgcaggagaa tgggatggtt ttgctttatt tacaacgggc
    10441 taaacgtgta gtatttgagt tcactgccgt acaggcagct tagaaattca caggtaacat
    10501 actccacccg cccaccatgt tcactgccgt acagacagat aaaatgcgaa aaaaaagctc
    10561 gcactttcgt acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat
    10621 acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa
    10681 ttgttttgct accaaacctc atgggtggca acggggcgct actataggga gttggagtaa
    10741 aacggtcaag aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc
    10801 agtatatcta catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca
    10861 caaaataaaa aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc
    10921 ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca
    10981 gttcaacagt cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg
    11041 agatgtgtgc agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca
    11101 acgtttcaag aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg
    11161 gggtatcact accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa
    11221 gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta
    11281 agccgcacca aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga
    11341 cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa ccaactctgc gaccgcaatt
    11401 ataacactct ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa acaagcatcg
    11461 tggtacccag aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattaaggta
    11521 atcccggcgg ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca
    11581 gtcgataagc ttaccgccat gaccgataaa ttcctcacag aataccagaa gcgccgtttt
    11641 agacgcattt tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac
    11701 gccgtacata ccgccgacaa gctcatcttc acgccagact tcaatggagt gggcatggcc
    11761 gagttcgtga aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc
    11821 gcgatcgctg gcacagcctt caatgacctg accaaaagcg taattcatcg tgacacgata
    11881 gggcgagcgt ttatgaaatc gcttcatact acggctgata tgcagtgatt ctggccatag
    11941 caccgcgcgg ggatcgggcg accaccagag gatggggtcg cctggagaaa accacggaaa
    12001 aataccacgc tggtaagcca ttaacaggcg cgcagggcta agatcgcccc caagtgccag
    12061 caggccgtta ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga
    12121 aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct
    12181 tacaaaccct gcttgaactg gtaataacgc ccctgtctgg caagcagctc tgcgtgagta
    12241 ccttgctcaa taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg
    12301 cgaagtcgat gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca
    12361 aggatctggc tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt
    12421 ggcgcatcat gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc
    12481 tggcgtccgc cttcacctaa ccaactgttg agacctgcat cctcgagcag cttctccaga
    12541 ccaacgcgac gcaagctctc cgccagagcc tcatcactac tgccaggcga ggcgagtaac
    12601 agattatcac gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc
    12661 tgtcgtagag ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc
    12721 tgttgcgggt cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt
    12781 cgcccgagaa tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc
    12841 tgttgggatt gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg
    12901 gcaacacgag tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg
    12961 atccgaacgg cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc
    13021 agtgcttcaa acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga
    13081 gcattgccgc caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca
    13141 atgagcagca ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc
    13201 agccattgaa tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg
    13261 gtcagctcag cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga
    13321 tgagtcagat tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa
    13381 agcgtcagta acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac
    13441 ccgattgtca ccatcataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg
    13501 taaagatgat cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga
    13561 tagcgcgcca gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga
    13621 tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca
    13681 gtacgggtga ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac
    13741 agtccggcaa cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacagt
    13801 ccgatactgg cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta
    13861 tgacgtttat acagtgctag atagggtagc aaagcgcgca tttaaatctc ctcctgacga
    13921 tgggccagta atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc
    13981 tcaataatcc ggccatcctg cataacccaa atgacatccc agtcagcaag atcttctaac
    14041 tggtgggtga ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg
    14101 cgctgttcac tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag
    14161 ggatttagta acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca
    14221 gcctggtcgc caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc
    14281 caggcgttat ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt
    14341 acgttatccc gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt
    14401 ttacgccatg attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc
    14461 tgatatgaga gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga
    14521 ccaaccaaca cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt
    14581 ttaccttccg gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat
    14641 gctaattcca cctcgccaca ctgcggatgg gcgagcgggg tttccataaa cgttttcaga
    14701 ctgtcagctg caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat
    14761 ggctggaaaa actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca
    14821 gtatcgtagt gaccaaaatc cagctcgcca agataggaaa aaccaaagta gaccgccact
    14881 agagcaattg acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt
    14941 agcacttcca ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca
    15001 gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc
    15061 ccacttaagc gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca
    15121 accagcgcca taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc
    15181 cagtttgagg ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt
    15241 tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc
    15301 gcccagctcc ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg
    15361 agaacctgcc ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg
    15421 cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc
    15481 agcagggctt cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac
    15541 caggcctggg caatgatcaa tatgccgctc acaaagccca gcagacgaga aatattcagc
    15601 caacgttggg agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat
    15661 ttattcattg cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac
    15721 ggggaaaaaa taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg
    15781 atttgtaaaa ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg
    15841 ccatgcagcc tgtaccggcc gaagtaatgg cctgacgata aatatgatcc atcacgtcac
    15901 ccgcggcaaa gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta
    15961 ctttgatgta gccgttttcc agttccagct gcccttcgaa aatggcagta ttcgggctgt
    16021 gaccgatagc aacaaacaga ccggcaacgt cgagtgattc gatgttatcg ctgttttgcg
    16081 tatcgcgcag acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtcc
    16141 ggttggtgtg cagaatgatg ttgccgttct ccactttatc catcagacgc ttaatgagga
    16201 ttttttccgc gcggaaaccg tcacggcggt gaatcagatg tacttccgaa gcgatgttag
    16261 acagatacaa cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt
    16321 tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt
    16381 cttcagaggg caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc
    16441 aagtgtattc gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt
    16501 tgatatgatc aaaaatgatt tcagtttcaa atttggtggc atgttcgtgc atgcgctcca
    16561 ttaataacgg accggtcaga tcgtttggat cgccaggcca gttttccact tctgtggtgg
    16621 tggtcagttg gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg
    16681 ccgcatagac agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt
    16741 gtttggtcgt gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg
    16801 aatttactga cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc
    16861 atcgataagc aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt
    16921 tataagctga ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact
    16981 aaaaatcgat gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa
    17041 aaaaaacagt attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta
    17101 gtgaaatcta cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc
    17161 aacggtcttc tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct
    17221 atcgtgacgg gtagcgactc tgaacagtga tgtttcaggg tcagacagga gtagggaagg
    17281 aatacagaga gacaataata atggtagata gcaagaagcg ccctggcaaa gatctcgacc
    17341 gtatcgatcg taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc
    17401 tttctaaacg tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa
    17461 gacaagggtt tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac
    17521 ttctggtatt cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca
    17581 ataccgctgt acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg
    17641 actacctgtt gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa
    17701 ccctgctgcg tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca
    17761 agcagagtaa tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt
    17821 attttgatta cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt
    17881 gtccgtttta gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga
    17941 atacactgaa gacaaagaag tcacattgac aaagttaagt agcggccgtc gccttctgga
    18001 agcgttgctg atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt
    18061 taacccttcg gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg
    18121 tgggatgccc ggtgcgtggc tggcagatac gctgttcttt atttttggcg tgatggctta
    18181 caccattccc gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagtga
    18241 cgaatacatt gattattttg ccgtttcgtt acgcatcatt ggcgttttgg cgctcatcct
    18301 tacctcctgt ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg
    18361 cgtcattggc agcttactaa gcactacgtt acaaccactg ctacacagta gcgggggaac
    18421 tattgcgctg ctctgcgttt gggctgcggg cctgacgctg ttcaccggtt ggtcatgggt
    18481 gaccattgct gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg
    18541 tacccgtcgc gatgatacct gggtcgatga agatgaatat gaagatgacg aagagtatga
    18601 agatgaaaac cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct
    18661 agcgcgtcgt aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc
    18721 tgcgttgttc tccggtaagc ggatggatga tgaagaagag attatctaca ctgcacgcgg
    18781 tgtggctgcc gacccggacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata
    18841 tgacgaatac gatccattat taaacggtgc gccaattacc gaacctgttg ctgtagcagc
    18901 tgctgctacc acggcgacac aaagctgggc tgcgccggtt gaacctgtga cccagacgcc
    18961 gcctgttact tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc
    19021 gggtccacaa acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc
    19081 acaatatgcg cagcctgcag tgcaatataa tgagccgctg caacaacctg tacagccaca
    19141 gcagccgtat tatgcgcctg cagctgaaca acctgtgcaa caaccttact acgctcctgc
    19201 ggcggaacaa cctgttcagc aaccgtatta cgcaactgcg ccggaacagc ctgcgcaaca
    19261 gccgtattat gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc aagccgaaga
    19321 gcagcaatcc acttttgctc cacagtctac ataccagact gagcaaactt atcagcagcc
    19381 agccgctcag gagccgttgt accaacagcc gcaacccgtt gaacagcagc ctgttgtgga
    19441 gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg ccgctttact attttgaaga
    19501 agtggaagag aagcgagccc gtgaacgtga acaacttgcg gcctggtatc aaccgattcc
    19561 agaaccggtt aaagaaccag aaccgatcaa atcttcgttg aaagcgcctt ctgctgcagc
    19621 agtacctcca gtagaagccg ctgccgctgt ttctccgctg gcatctggcg tgaaaaaagc
    19681 gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag
    19741 cggtggaccg cgtccgcaag tcaaagaggg aattggcccg cagttgccac gaccgaaacg
    19801 tattcgcgtg ccaactcgtc gtgaactggc gtcttacggt attaagctgc cctcacagcg
    19861 tgcggcggaa gagaaagccc gtgaagcccg gcgcaatcag tatgattctg gcgatcagta
    19921 taacgatgat gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt tcgcccagac
    19981 acagcagcaa cgctatggcg aacagtatca acatgatgtg cccgtaaacg cagaagatgc
    20041 agatgctgcg gcagaggctg aactggctcg tcagtttgcg caaactcaac aacaacgtta
    20101 ttccggcgaa caaccggctg gggcgaatcc gttctcgctg gatgattttg aattttcgcc
    20161 aatgaaagcg ttgctggatg atggtccaca cgaaccgttg tttacgccaa ttgttgaacc
    20221 tgtacagcag ccgcaacaac tggttgcacc gcagcagcag tatcagcagc cacaacagcc
    20281 ggttgcgcca cagcagcaat atcagcagcc gcaacagccg gttgcgccgc agccgcagta
    20341 tcagcagcca caacagcagg ttgcgccgca gccacaatat cagcagccgc aacaaccggt
    20401 tgcaccgcag cagcaggata ccctgcttca tccgctgttg atgcgtaatg gcgacagccg
    20461 tccgttgcat aaaccgacga cgccgctgcc ttctctggat ttgctgacac cgccgccgag
    20521 cgaagtggag ccggtagata cctttgcgct tgaacaaatg gctcgcctgg tggaagcgcg
    20581 tctggctgat ttccgtatta aagccgatgt cgtcaattac tctccggggc cggttatcac
    20641 tcgctttgaa cttaacctgg ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg
    20701 ggaccttgcc cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa
    20761 accctatgta ggtctggagt taccaaataa aaaacgacaa accgtttatc tgcgcgaagt
    20821 tttggataac gccaaattcc gcgataatcc gtcgccatta accgtggtgc tgggtaaaga
    20881 tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg
    20941 gactaccggt tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca tgctttataa
    21001 agcacagcca gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg agctttcggt
    21061 ttatgaaggc attccgcatc tgttaacgga agtcgttact gatatgaaag atgccgccaa
    21121 cgcgctgcgc tggtgtgtta acgagatgga gcgtcgttat aaactgatgt ctgcgctggg
    21181 tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc
    21241 gattccagac ccgtactgga agccgggtga cagtatggat gcccagcatc cggtgctgaa
    21301 aaaagaacca tacattgtgg tgttggttga cgaatttgcc gacctgatga tgacggtagg
    21361 taaaaaagtg gaagagctga tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca
    21421 cctcgtactg gcaactcagc gtccatcggt tgatgttatt actggtctga ttaaagcgaa
    21481 tattccgacc cgtatcgcct ttaccgtatc cagtaagatt gactcacgta ccattcttga
    21541 tcaggctggc gcggaatcac tgctggggat gggggatatg ctctactctg ggccgaactc
    21601 cacgttgccg gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc atgccgtggt
    21661 gcaggactgg aaagcgcgtg gtcgcccaca gtatgttgat ggcatcactt ccgacagtga
    21721 aagcgaaggt ggcgcgggcg gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca
    21781 ggcggtgcag tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt
    21841 ccgcattggt tataaccgtg cagcgcgtat tatcgaacag atggaagcgc aggggattgt
    21901 cagcgaacag gggcacaacg gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt
    21961 aatgcattgc cggataaggc gcggtagcgt cgcatccggc actctatcaa ctgaaaattc
    22021 agtattttct tttttcctca agctgattat tagcctggaa tagagagtag agggaactcc
    22081 cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa aattgccatc acctgtgcat
    22141 tactctcaag cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc
    22201 gcctggataa agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg
    22261 gtgcggcggt gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact
    22321 ggcatatgac acaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct
    22381 ataacccgtt cgttgagcaa gctacggcaa cttggctgaa agatgccacc ggtaatacgc
    22441 cgtttatgct gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga
    22501 atggcgatga ctttgtcctg acgccgaaag ccagcaatgg caatctgaag cagttcacca
    22561 ttaacgtggg acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc
    22621 gcagcagtta tcaactgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct
    22681 tcaccccgtc gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc
    22741 aatctgtcgc tcgatttttc ggataatact tttcaacctc tggccgcgcg tatgcggcca
    22801 gaaaatttag cacagtatat cggccagcaa catttgctgg ctgcggggaa gccgttgccg
    22861 cgcgctatcg aagccgggca tctgcattct atgatcctct gggggccacc gggtaccggc
    22921 aaaacaactc tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct
    22981 gccgtcacct ctggcgtgaa agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc
    23041 aatgcaggtc gccgcactat tctttttgtt gacgaagttc accgtttcaa caaaagccag
    23101 caggatgcat ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact
    23161 gaaaacccgt cgtttgagct taattcggca ctgctttccc gtgcccgtgt ctatctgttg
    23221 aaatccctga gtacagagga tattgagcaa gtactaactc aggcgatgga agacaaaacc
    23281 cgtggctatg gtggtcagga tattgttctg ccagatgaaa cacgacgcgc cattgctgaa
    23341 ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc
    23401 gaagtcgatg atagcggtaa gcgggtcctg aagcctgaat tactgaccga aatcgcaggt
    23461 gaacgtagtg cccgctttga taacaaaggt gatcgctttt acgatctcat ttccgcactg
    23521 cataaatcgg tacgtggtag cgcacccgat gcggcgctgt actggtatgc gcgaattatt
    23581 actgccggtg gcgatccgtt atatgttgcg cgtcgctgtc tggcgattgc gtctgaagat
    23641 gtgggtaatg ctgatccacg agcgatgcag gtggcaattg cagcctggga ttgctttact
    23701 cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc
    23761 gcgccaaaaa gcaacgctgt ctacactgcg tttaaagctg cgctggcgga tgctcgcgaa
    23821 cgtccggatt atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa
    23881 atgggctacg ggcaggaata tcgttacgct catgatgaag caaacgctta tgctgccggt
    23941 gaggtttact tcccgcggga aatagcacaa acacgctatt atttcccgac aaacaggggc
    24001 cttgaaggca agattggcga aaagctcgcc tggctggctg aacaggatca aaatagcccc
    24061 ataaaacgct accgttaatg ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg
    24121 caggctgtgg ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc
    24181 aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag
    24241 ctggatgtag ataagctggg cgctctcgaa gagcgtcgta aagtattgca ggtcaaaacg
    24301 gaaaacctgc aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc
    24361 ggggaagata tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca
    24421 gcaaaagccg agctggatgc tttacaggct gaaattcgcg atatcgcgct gaccatccct
    24481 aacctgcctg cagatgaagt gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc
    24541 cgctggggta ccccgcgtga gtttgacttt gaagttcgtg accacgtgac gctgggtgaa
    24601 atgcactctg gcctcgactt tgcagctgca gttaagctga ctggttcccg ctttgtggta
    24661 atgaaagggc agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat
    24721 accgaacagc atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg
    24781 tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt tccatactcg tccgctggaa
    24841 gaagaagcag acaccagtaa ctatgcgctg atcccaacgg cagaagttcc gctgactaac
    24901 ctggtgcgcg gtgaaatcat cgatgaagat gatctgccaa ttaagatgac cgcccacacc
    24961 ccatgcttcc gttctgaagc cggttcatat ggtcgtgaca cccgtggtct gatccgtatg
    25021 caccagttcg acaaagttga aatggtgcag atcgtgcgcc cggaagactc aatggcggcg
    25081 ctggaagaga tgactggtca tgcagaaaaa gtcctgcagc tgctgggcct gccgtaccgt
    25141 aaaatcatcc tttgtaccgg cgacatgggc tttggcgctt gcaaaaccta tgacctggaa
    25201 gtatggatcc cggcacagaa cacctaccgt gagatctctt cctgctccaa cgtttgggat
    25261 ttccaggcac gtcgtatgca ggcacgttgc cgcagcaagt cggacaagaa aacccgtctg
    25321 gttcataccc tgaacggttc tggtctggct gttggtcgta cgctggttgc agtaatggaa
    25381 aactatcagc aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac
    25441 ggactggaat atattggcta atacccaatt tttctgaatc taaaaagcgc ctgcgggcgc
    25501 tttttttgtc tccctttgat accgaacaat aattactcct cacttacacg taatactact
    25561 ttcgagtgaa aatctaccta tctctttgat tttcaaatta ttctatgtat acaagcctat
    25621 atagcgagct gctatagaaa taattacaca atacggtttg ttactggaat caatcgtgag
    25681 caagcttgag tgagccatta tgaaaacgaa aatccctgat gcggtattgg ctgctgaggt
    25741 gagtcgccgt ggtttggtaa aaacgacagc gatcggcggc ctggcaatgg ccagcagcgc
    25801 attaacatta ccttttagtc ggattgcgca cgctgtcgat agcgccattc caacaaaatc
    25861 agacgaaaag gttatctgga gcgcctgtac agttaactgt ggtagtcgct gcccgctacg
    25921 tatgcacgtc gtggacggtg aaatcaaata tgtcgaaacg gacaataccg gcgatgacaa
    25981 ttacgacggc ctgcactagg ttcgcgcctg cctgcgtggg cgttccatgc gtcgccgtgt
    26041 ctacaatccg gaccgcctga aatatccgat gaaacgagtc ggggcgcgcg gtgaaggcaa
    26101 attcgagcgc attagctggg aagaagccta cgacatcatc gcgaccaata tgcagcgcct
    26161 gatcaaagag tacggcaacg agtctatcta tctgaactat ggcaccggta cgctgggcgg
    26221 caccatgacc cgctcctggc cgccgggaaa taccctggtc gcgcggctga tgaactgctg
    26281 cggcggctat ctgaaccatt acggcgacta ctcctccgcg caaattgcgg aaggtttgaa
    26341 ctatacctac ggcggctggg cagatggcaa cagcccgtcg gatatcgaaa acagtaagct
    26401 ggtagtgctg tttggtaata accctggcga aacgcgaatg agtggcagtg gggtgactta
    26461 ctatcttgaa caggcacgcc agaaatctaa tgcccgcatg atcatcatcg atccgcgcta
    26521 taccgacacc ggtgccgggc gcgaagatga gtggatccct attcgtccgg gaacagatgc
    26581 cgcactggtt aacggtctgg cgtacgtcat gatcactgaa aacctggtgg atcaggcatt
    26641 cctcgataaa tattgcgttg gctacgatga gaaaaccctg ccagccagtg cgccgaaaaa
    26701 tggccactat aaagcttata ttctgggtga agggccagat ggcgtggcta aaacaccgga
    26761 atgggcctca caaatcaccg gtgttccggc agacaaaatc atcaaactgg ctcgtgaaat
    26821 cggcagtacc aaaccggcgt ttatcagcca gggatggggc ccgcagcgtc acgctaacgg
    26881 tgaaatcgca acccgtgcta tctcgatgct ggcgattctg accggtaacg ttggtattaa
    26941 cggaggcaac agcggcgcgc gtgaaggttc atacagctta ccgtttgtcc gtatgccgac
    27001 cttggaaaac ccgatccaga ccagcatttc gatgtttatg tggaccgatg ccattgaacg
    27061 tggcccggaa atgacggcgc tgcgtgatgg tgtacgcggg aaagataagc tggatgtgcc
    27121 gatcaaaatg atctggaact atgccggtaa ctgcctgatt aaccagcatt ctgaaatcaa
    27181 ccgtacccat gaaatccttc aggatgataa gaagtgcgag ctgattgtgg ttatcgactg
    27241 ccacatgacc tcatcggcga aatatgctga catcctgctg cctgactgca ccgcttccga
    27301 acagatggac tttgcgctgg atgcatcctg cgggaatatg tcttacgtga ttttcaacga
    27361 tcaggtgatt aaaccgcgct ttgaatgtaa gaccatctat gaaatgacca gcgaactggc
    27421 aaaacgtctt ggcgttgagc aacagtttac tgaaggccgt acacaggaag agtggatgcg
    27481 gcatctgtat gcccagtcgc gggaagcgat tcctgaacta ccaacgtttg aagagttccg
    27541 caagcagggg atctttaaaa agcgcgaccc acaagggcac cacgttgctt ataaagcttt
    27601 ccgtgaagat cctcaggcaa attcactgac cacgccatcg ggcaaaattg agatttattc
    27661 gcaggcgctg gctgacattg ccgctacctg ggaattgcca gaaggagatg tgatcgatcc
    27721 actgccgatc tacacgccgg gctttgaaag ttatcaggat ccgttgaaca aacagtatcc
    27781 gctgcagctt acgggtttcc actataaatc tcgcgttcac tcaacttacg gcgacgttga
    27841 tgtgctgaaa gcagcttgcc gtcaggaaat gtggatcaac ccgcttgatg cgcaaaaacg
    27901 cggtatcaac aacggcgata aagtcaggat ctttaacgat cttggtgagg ttcatattga
    27961 ggcgaaagtg acgccacgaa tgatgccggg tgtggtcgca ctgggtgaag gtgcctggta
    28021 tgacccggat acaaaacgtg tcgataaggg cggttgtatc aacgtactga ccactcaacg
    28081 tccgtctcct ctcgctaagg ggaatccgtc acatacaaac cttgttcagg ttgaaaaggt
    28141 gtaaggagta accgatgaca acccagtatg gattttttat tgattccagc cgttgcaccg
    28201 gttgcaaaac ctgcgagctg gcctgtaaag actacaaaga tttgacgcca gaagtcagct
    28261 tccgccgcat ttatgaatat gctggcggcg actggcagga agataacggt gtctggcacc
    28321 agaacgtgtt tgcctactat ctgtcgattt catgtaacca ctgcgaagat ccggcttgta
    28381 ctaaagtctg cccgagcggt gcgatgcata aacgtgaaga tggttttgtt gtggtcgatg
    28441 aagatgtgtg cattggctgc cgctactgcc atatggcttg cccgtatggc g
//
LOCUS       DADLTD010000028        28208 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLTD010000028 DADLTD010000000
VERSION     DADLTD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.028"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            44..1270
                     /gene="dsdA"
                     /locus_tag="JN701_000995"
     CDS             44..1270
                     /gene="dsdA"
                     /locus_tag="JN701_000995"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY7363517.1"
                     /translation="MAEGLPYVGLTEQDVQDAHARLSRFAPYLAKAFAETAATGGIIE
                     SELVAIPAMQKRLEKEYQQPISGQLLLKKDSHLPISSSIKARGGIYEVLAHAEKLALE
                     AGLLTLDDDYSKLLSPEFKQFFSQYSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADA
                     RAWKKAKLRSHGVTVVEYEQDYGVAVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAG
                     QRLKAQFAQQGRIVDADNPLFVYLPCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSP
                     CMLLGVHTGLHDQISVQDIGIDNLTAADGLAVGRASGFVGRAMVRLLDGFYTLSDQTM
                     YDMLGWLAQEEGIRLEPSALAGMAGPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGG
                     GMVPEEEMNQYLAKGR"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="JN701_000996"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="JN701_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY7363518.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="JN701_000997"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="JN701_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY7363519.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="JN701_000998"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="JN701_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY7363520.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="JN701_000999"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="JN701_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY7363521.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="JN701_001000"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="JN701_001000"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY7363522.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="JN701_001001"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="JN701_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY7363523.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIVYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="JN701_001002"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="JN701_001002"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY7363524.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="JN701_001003"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="JN701_001003"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY7363525.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15139)
                     /locus_tag="JN701_001004"
     CDS             complement(14507..15139)
                     /locus_tag="JN701_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY7363526.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15435..15710
                     /locus_tag="JN701_001005"
     CDS             15435..15710
                     /locus_tag="JN701_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363527.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15787..16029)
                     /locus_tag="JN701_001006"
     CDS             complement(15787..16029)
                     /locus_tag="JN701_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY7363528.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16382..17302
                     /gene="lpxP"
                     /locus_tag="JN701_001007"
     CDS             16382..17302
                     /gene="lpxP"
                     /locus_tag="JN701_001007"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY7363529.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17658..17729
                     /gene="ypdK"
                     /locus_tag="JN701_001008"
     CDS             17658..17729
                     /gene="ypdK"
                     /locus_tag="JN701_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY7363530.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="JN701_001009"
     CDS             complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="JN701_001009"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY7363531.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19409..21106
                     /locus_tag="JN701_001010"
     CDS             19409..21106
                     /locus_tag="JN701_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7363532.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21121..21855
                     /locus_tag="JN701_001011"
     CDS             21121..21855
                     /locus_tag="JN701_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7363533.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21868..22725
                     /locus_tag="JN701_001012"
     CDS             21868..22725
                     /locus_tag="JN701_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7363534.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22728..25223)
                     /gene="ptsP"
                     /locus_tag="JN701_001013"
     CDS             complement(22728..25223)
                     /gene="ptsP"
                     /locus_tag="JN701_001013"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7363535.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25248..26285)
                     /gene="ypdE"
                     /locus_tag="JN701_001014"
     CDS             complement(25248..26285)
                     /gene="ypdE"
                     /locus_tag="JN701_001014"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY7363536.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26285..27370)
                     /gene="ypdF"
                     /locus_tag="JN701_001015"
     CDS             complement(26285..27370)
                     /gene="ypdF"
                     /locus_tag="JN701_001015"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY7363537.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27385..28203)
                     /locus_tag="JN701_001016"
                     /pseudo
     CDS             complement(27385..28203)
                     /locus_tag="JN701_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         7541 a         6905 c         6437 g         7325 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagttg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaagggggaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataaacaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    15061 ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag
    15121 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    15181 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    15241 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc
    15301 ctgtcgcaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    15361 atattgatta attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt
    15421 caggataact ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggtctata
    15481 ttatggcatg caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata
    15541 aagagactga aaaaacatca gcgatgacga ccacacactc tacaaaactt cagccatcac
    15601 aggcgatttt gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca
    15661 cgcctactta tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    15721 atggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct
    15781 gacccactat cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    15841 aagaaacata aatatctccg tcactagttc attaccgcca gttaaccaca tggtgaagta
    15901 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    15961 cagtacctga ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa
    16021 ataaatcata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    16081 gtaagacaat ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa
    16141 atgcaagact gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga
    16201 aagcagtcag cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    16261 gactagtctt tacacacttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    16321 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    16381 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    16441 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    16501 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg
    16561 taaaaacctg gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    16621 cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc
    16681 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca
    16741 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    16801 ccgcgtgatg ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct
    16861 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    16921 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    16981 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    17041 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    17101 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    17161 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    17221 catgcgcgca ccggagcagt acctctggat ccactgtcgc tttaaaacgc gcccggtggg
    17281 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    17341 aataacctca tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa
    17401 ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta
    17461 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    17521 agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct
    17581 ttgcccagtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt
    17641 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    17701 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    17761 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    17821 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg
    17881 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc
    17941 aaagccaatc cccggcgaga cacagacctt cgcttcgtta agcaatttct tggcaaatac
    18001 cagcgagccc atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt
    18061 cggcatttca actatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg
    18121 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    18181 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc
    18241 gctgaccagc gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct
    18301 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    18361 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
    18421 atcgtagcgt ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc
    18481 ggttggattc gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
    18541 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    18601 cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac
    18661 cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
    18721 agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg
    18781 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtcctg
    18841 gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    18901 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    18961 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    19021 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    19081 cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    19141 catttttatc aatggcttta aatgaggaac tccacttcaa aaacaggaaa tttttctacg
    19201 ctaacaagaa aattaccacc aattttatta acattataat ttcaataaat ttaccagcca
    19261 gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa
    19321 atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    19381 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    19441 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    19501 tcgcgagctg ttgcacaagt cggcacactc cccaaaagag ttgcttgctg ttaccgccat
    19561 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    19621 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat
    19681 cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc
    19741 gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa
    19801 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt
    19861 gaccatgatt ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    19921 taaaatcggc attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt
    19981 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
    20041 tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt
    20101 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca
    20161 tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
    20221 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    20281 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    20341 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    20401 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    20461 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    20521 ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc
    20581 tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    20641 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    20701 ggcccgcttt ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    20761 cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc
    20821 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat
    20881 tgcggtgcga gataccgggc acggcatcga tccaaaggtg attgagcggg tcgaagcgaa
    20941 tgaaatgccg ggcaataaaa ttggcctgct gaatgtccat catcgcgtga agttattgta
    21001 tggcgagggg ctgcatatcc gccgcctgga gccggggaca gaaattgcgt tttacattcc
    21061 taaccaacgc accccagtcg cctcacaggc tacgttattg ctttgagccg gagtgatatt
    21121 gtgaaagtca tcattgttga agacgaattc ctggcacaac aggaactgag ctggctaatt
    21181 aaagagcaca gccagatgga gattgtcggc acctttgacg acggtctgga cgtgttgaag
    21241 tttttgcagc ataaccgcgt cgacgccatt tttctggata tcaatattcc gtcgctggat
    21301 ggcgtgttgc tggcgcaaaa catcagccag ttcgcccata aaccgtttat tgtgttcatc
    21361 accgcgtgga aagaacatgc ggtagaagcg tttgaactgg aggcgtttga ctacattctc
    21421 aaaccgtatc aggagtcacg tattaccggg atgctgcaaa aactggaagc ggcctggcaa
    21481 caacagcaga ccagcagtac gcctgccgcg acggtaacgc gtgagaatga caccattaat
    21541 ctggtgaaag atgagcgaat aatcgtcacg ccaattaacg atatctatta cgccgaagcg
    21601 cacgagaaaa tgacctttgt ctatacgcgg cgtgaatcct acgtaatgcc gatgaacatt
    21661 accgaatttt gcagcaaact gccgccgtcg cattttttcc gctgccatcg ctcattttgt
    21721 gtcaatctga acaaaatacg cgaaatcgaa ccgtggttta ataacaccta cattctgcga
    21781 ctgaaagatc tggattttga agtgccggtc agccgcagca aagtgaaaga atttcgccag
    21841 ttaatgcatc tttaatcggg aactttcatg aaagcaccag gcttgcccgc cgatcaacaa
    21901 tttttcgccg atctgttcag cggcctggtg cttaactcgc aactactggg gcgcgtctgg
    21961 tttgccagcc agcctgcctc gttaccggtg ggcagtttat gtattgattt tccccgtctg
    22021 gatatcgtgc tgcgcggcga atacggcaac ctgctggaag caaagcagca gcgaatggtg
    22081 gaaggagaaa tgctgtttat tccggcacgc gcggctaatt taccgatcaa caacaaaccg
    22141 gtgatgctgt taagcctggt gttcgcaccg acctggcttg ggttatcgtt ttacgatagc
    22201 cgcaccacgt cgttgttgca tcctgctcgc cagatccagc ttcccagcct gcaacgcggt
    22261 gaaggtgaag cgatgcttac cgccctcact catcttagcc gttcgccgct ggagcagaat
    22321 atcattcagc cactggtgtt aagtttgctg catctttgcc gcaacgtggt gaatatgccg
    22381 ccgggtaatt cgcaaccgcg cggcgatttt ctctatcaca gcatttgtaa ctgggttcag
    22441 gataattata cccagccgct cacccgcgag agcgtggcgc agttttttaa tatcacgccc
    22501 aatcatctgt caaaactgtt tgctcagcat ggaacgatgc gttttatcga gtatgtgcgt
    22561 tgggtgcgaa tggcgaaggc gaggatgatt ttgcagaaat atcatctgtc aattcatgaa
    22621 gtggcacagc gttgcggttt tccggatagc gactattttt gtcgcgtttt ccggcgtcag
    22681 tttggtctga cgccgggaga gtacagcgcc cgttttcagg gctaacgtca gaaggttaat
    22741 tctgtttcca gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa cgactggcgg
    22801 aagtttttat tcaccagttt acgcgccagc tgcgagaaga ctttcacatg attcatccct
    22861 tcgttagcac ccagcgtcag catgatcacc agttcgactt cgcccatttc tgactgccag
    22921 tcaatcggtt tcgccagccg ggcaatgctg atactggaat gacggatcca ctgagatttg
    22981 gtgtgcggga tcgctacgcc aaaaccaacg ccggtggtaa caatctcttc ccgctgccag
    23041 acatcttctt ccagctcaaa cggatgttca gtgcgcccgt taacgccgag gttgccgcac
    23101 aggaactgga tcgcctgctc tttattgcta aaatcctgat caacaaagat attctccagc
    23161 gccagcagtg ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc ttcaatttcc
    23221 tgcgcactgc ggcattcaca tgcctgacgc gccagttccc gacacgcctc gctatccagt
    23281 tgacgaagct ggcttttcac cgccggaata cgcgggctac tcatactcag ctcatccagg
    23341 cccagcccaa gcagtagcgg cagataacgg ctttcaccgc ccagttcacc gcaaatgcct
    23401 acccatttgc cccgctgatg cgcagtggta actatttgct gcaacatgcg caggaacgat
    23461 ggcgtaatcg ggttatatag cggcgatacg cgcgggttat tacgatcgac cgcatacaga
    23521 tactgggtca tatcgttgga gccgatactg aagaaatcga cctcatcgca gaagtggtcg
    23581 atgatgtagc acaccgacgg cacttccacc atgatcccaa gcgtaatcgt ctctgcatga
    23641 cgcaggccat cgcgcttaag ctcaacgatc gctttttgga tctcgccctt cacccataag
    23701 atctgatcga gactgtggac catcgggatc atcaactggg cgttgccaaa actggcggcg
    23761 cgcaaaatcg cccgtagttg agtgcggaac aggccagcaa attccgggta aatacgtacc
    23821 gcgcgatagc cgaggaacgg gttctcttcc tggggaatat tcagataagg aatgctttta
    23881 tcgccaccga tgtccattgt gcggaagata atcggcttgt cgcccgctgc cagtagcacc
    23941 tgctggtagg cttcaaattg ctcctgctcg tccggcgcgc tgtcgcgatc catatagagc
    24001 atttcagtac ggaacagtcc aacgccttcc gcgccgttgg caaacgcgcc tggcgcttcc
    24061 agagcggtgc cgatattcgc cgcaatatca atacgcttgt tatcacggga ataggccagc
    24121 tgcgcggcgg cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg tgcgacctga
    24181 taataaccgc tcaccgcgtc attcgggtta atcgccagca cgccgcactg ggcgtcaagc
    24241 accgccggtt gcccggcata acgggcaatc gcatccagcg gcaggccgct cagtaccggg
    24301 atcgccgagg cgcgggccag aatcagggta tgcgaggtgc gcccagtttt ctccagaatc
    24361 atgcctgcaa gattttttaa atcgaggctc aaaaactggc ttggggttaa atcctcggct
    24421 accagaatag tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc cggccaggtg
    24481 atatgcagca actgttcgct aatatcgcga atgtcgctaa cacgttcgcg cagataatca
    24541 ctggcagaag cagaaagttt ggcgcaaacc tgctccatat tgctgatgat cgccgccccc
    24601 agcccttgat gctgttctgt catcagacga cggatattgc ccgcgaattc atcatcctga
    24661 atcagcgaca aatgggcgct aaggatagtt ttgctttcgc cgtcactctc acgcaattgc
    24721 tggttcagtt gctcggcaag cgttgcaagg ctgtgctcca gccgggtgga atcttgcgca
    24781 ctggcgggga ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt cagcgtaccc
    24841 acgccgacgc tgcttgccag cacattgccg tacagtaaat ccgggttcag gcggcttaat
    24901 gaacgcggca gcggatgcgc cgtcagttct gcctgcgttg gctggatgct gtcgctgtcg
    24961 ataaagcgca cctggatgta ctcttccaac acgcgctgcg cctgctcttc atcgctgccg
    25021 ctaatgttca ggctgcaact gtcattaaat aaagtaccgg tgccaatcag cgccagcgag
    25081 cttttcgcat cggcctttgc gttctggcga tggttgataa aggtgatttc gctttgccac
    25141 tggctgcact gttctttaag ttcccacgcc ggacgagcat gaagaccgtt tggcaaagga
    25201 cagagaaatt gaatcgttaa cataaccgct ccgggttaat caggagatca tctgaaatcc
    25261 gtcagttgaa caaccgtctc acgcgtaaga cgtttaataa gggcagataa aagctgctgc
    25321 atctgcaaaa tgtcacggca atcggcaatc gatgcggcgc aatgtccgtg gcgggttgct
    25381 ggccccatca ccactgtagg cacgccggtg ccggttaagt gcaccgctcc gccgtccgtg
    25441 ccgccgttgc tgaacatatc tgcctgtaac ggcacgccaa tttctgccgc cacggtttcg
    25501 atccacgcgg taagttttgg cggcgcaatc agcgacttgt cgcttaacac cagcatcggc
    25561 ccgttacaaa tctgacgatg gttagccgcg ccataatcaa agtttttcgc ccagcaggcg
    25621 gtatcaagca caatggcgac atccggcgac accgcgcggg tggcagtttg cccgccgcgt
    25681 acccccacct cttcgctgga actggccacc agccacactt ccgcaggcag ttcagcgctg
    25741 tgtagttcgc gcagtaacgt caccagcagg tagcaaccga ggcggtcatc aaaggctttc
    25801 cccatcaccc gctggtgagg gagaacctga aaagttgtat caaaggtgac gcgatcgcct
    25861 ggacgaattc ccgcctgcat cacttcgtca tacgagcgcg taccaatgtc cacgcgcatg
    25921 gcgctgacgt cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca
    25981 cgggtggtga tgcgcaccgg ctgcagctgg cgggcagcca tgcgcacgtt gccaaccggc
    26041 agcacatcaa ttgccccttc gcgggagatg ctgcgcacca taaatcccac ttcgtccata
    26101 tgggcgcaga tcatcacctt cggccctgtc gattcattga ggcggatcag caccgatccc
    26161 agaccatcaa atcgcacttc tttttgcagg cgatccgctt cttccagcag gatctgccgc
    26221 acttcctgtt ccgaggaggc gatcgcatct gcctcgctca acgcttttaa tagcgataaa
    26281 tccattatgc ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc
    26341 cttgcggggt aaccagcaca acatcttcga tgcgcacgcc cccttgccct ggcaaataaa
    26401 tccccggctc cacggtcagc aacatgcctg gctgtagcgt cgtggtgtcc cgcggtgaaa
    26461 aacgcggatc ttcatggact tcaatgccga tagcgtgacc agtgttatga ccgaaatagt
    26521 cgccaaatcc agcctcggta atgacccggc gcgcggcgtc gtcaatctgc tggcagcgca
    26581 cgccggggcg aattgcagag attgccgcga gctgtgcctg caggacaatc tgatagacgt
    26641 caaacagcgg gtgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca
    26701 tatcagagca gtagccctga tacagcgcac caaaatcgag agtgacaaac tcgcccgctg
    26761 caacaatctt gtcgctggct ttgccgtgcg gcagcacccc gcgccagccg ctggcgacaa
    26821 tggtgtcaaa agaggctttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg
    26881 ctatctcgcg ctcgctcatc cccgcctgaa taaagcggcg aatatgctcc gctccgcgat
    26941 cggcaatccc acaggcgagg cggattatct ccacctcctc tggcgttttg atttgccgca
    27001 gcacatccgg cgtggcgcta accagtttcg cattgagttc agacttccag cggtgcgcgg
    27061 tttcccaact cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga
    27121 tttgattgac gatagtggta agcgtgttcg tcgcgtcaag caaatgcagc tggtagcctt
    27181 gcgcacgggc ttccacctca acgaaatagc tcgaatctac cagaatgtgc gcactttcac
    27241 ggctaataac cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc
    27301 gtgaggagag aagcactgca tccagttgtt gctccttaag ccagtcgcgc agcgaagcga
    27361 gtaatgtcat ctggattttc cttcttacag gctatcgatt aacaatttgc ctttacggaa
    27421 catcatcaga cgcaggaaca ccaccatcag cgcagttatg actgccccca gcgcgatccc
    27481 cgccatatag acgccaaggt tagtgaccag cggccatgcc cagatagcgg attccgggaa
    27541 ccattgcact gcgcccagcc agacagcggc ggttgagccg acaatcgcgc cgaccatata
    27601 cgacggaatg gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactaatccc
    27661 cataaaggca aggaacatcg cggtcttacc ctgcggataa agttgagcgt tgaacaggcg
    27721 tttgccggtt aaacgacggt caataatggt cgccagcccc agaccaatcg gaggaataac
    27781 gatagcgata gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac
    27841 gaaacctgcg gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc
    27901 cattgcgtac atcagcgcgc ccttctcacc tgcggcagtc agcacggtac ggataacgcc
    27961 gttgatccag ccgccaaagg gggtaatgac gtagtacatc gccagcatga caaaaatggc
    28021 ggaaagaatc ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat
    28081 tttctggttc atccacttca caaggtaacc cgcgacgata gaaataatca gcgcaccaat
    28141 gaaggtggac ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag
    28201 cagttacg
//
LOCUS       DADLTD010000029        28177 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLTD010000029 DADLTD010000000
VERSION     DADLTD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.029"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(265..1365)
                     /gene="chaA"
                     /locus_tag="JN701_001017"
     CDS             complement(265..1365)
                     /gene="chaA"
                     /locus_tag="JN701_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY7363538.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1635..1865
                     /gene="chaB"
                     /locus_tag="JN701_001018"
     CDS             1635..1865
                     /gene="chaB"
                     /locus_tag="JN701_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY7363539.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2023..2718
                     /locus_tag="JN701_001019"
     CDS             2023..2718
                     /locus_tag="JN701_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7363540.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2762..3115)
                     /gene="ychN"
                     /locus_tag="JN701_001020"
     CDS             complement(2762..3115)
                     /gene="ychN"
                     /locus_tag="JN701_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY7363541.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3299..4693
                     /gene="ychO"
                     /locus_tag="JN701_001021"
     CDS             3299..4693
                     /gene="ychO"
                     /locus_tag="JN701_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY7363542.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYFTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLLANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRVGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEYNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4694..5344)
                     /gene="narL"
                     /locus_tag="JN701_001022"
     CDS             complement(4694..5344)
                     /gene="narL"
                     /locus_tag="JN701_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY7363543.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5337..7133)
                     /gene="narX"
                     /locus_tag="JN701_001023"
     CDS             complement(5337..7133)
                     /gene="narX"
                     /locus_tag="JN701_001023"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY7363544.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7472..8863
                     /gene="narK"
                     /locus_tag="JN701_001024"
     CDS             7472..8863
                     /gene="narK"
                     /locus_tag="JN701_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY7363545.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPVFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9379..13101
                     /locus_tag="JN701_001025"
     CDS             9379..13101
                     /locus_tag="JN701_001025"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7363546.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13119..14657
                     /gene="narH"
                     /locus_tag="JN701_001026"
     CDS             13119..14657
                     /gene="narH"
                     /locus_tag="JN701_001026"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7363547.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14654..15364
                     /gene="narJ"
                     /locus_tag="JN701_001027"
     CDS             14654..15364
                     /gene="narJ"
                     /locus_tag="JN701_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY7363548.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15364..16041
                     /gene="narI"
                     /locus_tag="JN701_001028"
     CDS             15364..16041
                     /gene="narI"
                     /locus_tag="JN701_001028"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY7363549.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16224..16355)
                     /locus_tag="JN701_001029"
     ncRNA           complement(16224..16355)
                     /ncRNA_class="other"
                     /locus_tag="JN701_001029"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16403..16487)
                     /locus_tag="JN701_001030"
     tRNA            complement(16403..16487)
                     /locus_tag="JN701_001030"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16451..16453),aa:Tyr,seq:gta)
     gene            complement(16647..17489)
                     /gene="purU"
                     /locus_tag="JN701_001031"
     CDS             complement(16647..17489)
                     /gene="purU"
                     /locus_tag="JN701_001031"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY7363550.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17539..17997)
                     /locus_tag="JN701_001032"
     CDS             complement(17539..17997)
                     /locus_tag="JN701_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY7363551.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            18110..19015
                     /gene="rssA"
                     /locus_tag="JN701_001033"
     CDS             18110..19015
                     /gene="rssA"
                     /locus_tag="JN701_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY7363552.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            19107..20120
                     /gene="rssB"
                     /locus_tag="JN701_001034"
     CDS             19107..20120
                     /gene="rssB"
                     /locus_tag="JN701_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY7363553.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20322..21230
                     /gene="galU"
                     /locus_tag="JN701_001035"
     CDS             20322..21230
                     /gene="galU"
                     /locus_tag="JN701_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY7363554.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21374..21787)
                     /gene="hns"
                     /locus_tag="JN701_001036"
     CDS             complement(21374..21787)
                     /gene="hns"
                     /locus_tag="JN701_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY7363555.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22392..23009
                     /gene="tdk"
                     /locus_tag="JN701_001037"
     CDS             22392..23009
                     /gene="tdk"
                     /locus_tag="JN701_001037"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY7363556.1"
                     /translation="MAQLYFYYSAMNAGKSTVLLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23291..24187)
                     /locus_tag="JN701_001038"
                     /pseudo
     CDS             complement(23291..24187)
                     /locus_tag="JN701_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24311..26986)
                     /gene="adhE"
                     /locus_tag="JN701_001039"
                     /gene_synonym="adhC"
     CDS             complement(24311..26986)
                     /gene="adhE"
                     /locus_tag="JN701_001039"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY7363557.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            27464..28111
                     /gene="ychE"
                     /locus_tag="JN701_001040"
     CDS             27464..28111
                     /gene="ychE"
                     /locus_tag="JN701_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY7363558.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         6919 a         6776 c         7342 g         7140 t
ORIGIN      
        1 taaagccccc gcaacattgc gcctcaccgg ataattccgg cttggtgtgg atactacgtc
       61 tcaattcatc ttcacttcat ccctgaaatg tttgcaataa agagtacatt ccggcttttc
      121 aacagctgtt gcagtggttt cgtgcaggtt gtggtacagg ctcgcaattc tgattaacga
      181 gctgtccagg tagtatgaag aaatacaata agcaggaatt tatcttatct cccccgattt
      241 ccgtcatcgg aggagatata actgtcaggc aaatatcgtc atcaaatagg cggcaaacag
      301 tgccagatgc gctgcgccat tgagcacgtt agtacgtccg gtggagaagg agatatggca
      361 cagcactaaa gaggccacca tcaccaccat ttctggcgca ccaagtgcaa actgtaattc
      421 gttacccgtc ataaaggcaa ttagcgtgac gacaggtacg gtaagtgaaa tggttgctaa
      481 cactgaacca aagaacagat tcatcgcgcg ctggacctgg ttgttcaaca ctgcttttaa
      541 cgcacctaaa ccttccggcg acagaatcaa cagagccacc aggaagccag taaaggcgac
      601 aggggcattc atgctgtcga gcaatgtctc cagcgggctg gcattcattt tggtgactgc
      661 aataacggca atcagatgga taatcaacca gatagcatgc cacacgctgc tatgggcaga
      721 cggtttaccg tgatgcgggt cgtcgtcatc actgtcatct tcgtgctcgt agacaaacaa
      781 actttgatgc gttttggtct ggatcagcaa aaatacgcca tacattgccg cagaaattaa
      841 tgctaccagt aacgcctgac cggttgaaaa attcgccgca ggcagagcca ttggaaatac
      901 cagtacgatt atcgccaggg ggaacagcgc aattaaatac tgcttgatac caaacagatt
      961 catatattgg gtggcaaact tacgaccgcc caacaataat gaaaagccaa ccagcccacc
     1021 ggtaacaatc ataatgattg aatagagcgt atcacgcatt agcgttggcg cggcgtcgcc
     1081 ggttgccatt aaagctgaaa tcaaactgac ttcaagaata accactgaaa ggctaagaat
     1141 aagcgaaccg taaggttctc ccaggcgatg ggctaatacg tccgcatgac ggacaacact
     1201 aaaggcgcta cttaaaatac caataagcgc aagaagattg atggcaatga ccactggtag
     1261 tgtctggctg cttccccaca ggaacagcac taccagcgcc agaaccggga aaataagcga
     1321 aatctccttg tggcgggttt ttaccgcctc ttgagcattt gacattatgg ttatcccttt
     1381 gcagatgaat ttatcgaaaa tgtaaaaaat aggtaggaaa aataacagaa agtgtctgga
     1441 tatcggtaac attttacgaa tttttacccc actgtcgatt ttttactcat tgggacataa
     1501 aaataagtat tacgtttaga caatgtttgt tttagcgttt cttaagaaca gtctgacatg
     1561 aaaattctta tgttttggca agagtagata ttgttgacca cacttaatgt tcaactttgt
     1621 aaaaggagtc aacgatgccg tataaaacga aaagcgatct gccggaaagc gtaaagcacg
     1681 ttctaccgtc tcatgcccag gatatctata aagaagcgtt caacagcgca tgggatcaat
     1741 ataaagataa agacgatcgg cgtgatgacg ccagtcgcga agaaaccgcg cataaagtgg
     1801 cctgggctgc tgtgaagcat gaatatgcca aaggggatga tgataaatgg cataaaaaat
     1861 cgtaaaaccg gtagcttagt taaagctatt cgtgcggtgt tgccttgcaa gtggtccgtg
     1921 gattgcatat tgtcccgtta gtggtttcaa aatgagcagt aaaaatgtcc ggaagacacc
     1981 aaaaagttgt cgcagggaag tatgcagtgg cggaggtgta aggtgataac gcgtgatttc
     2041 ttgatgaatg ccgattgtaa aacggcattt ggtgccattg aagaatcact cttatggtca
     2101 gcagagcaac gagcggcttc gctggcggcg acgctggctt gtcgacctga tgagggaccg
     2161 gtgtggatct tcggttatgg atcgttgatg tggaatccgg cactggagtt taccgagtcg
     2221 tgcaccggta cactggttgg atggcatcgc gcgttttgcc tgcgcctgac cgccgggcgc
     2281 gggactgcgc accagccggg acggatgctt gcactgaaag agggcggacg cactacaggc
     2341 gtcgcctatc gactgccaga agagacgctg gagcaggaac taaccctgtt gtggaagcgc
     2401 gagatgatta ccggctgtta tctgccaacc tggtgtcagc ttgatcttga tgatggatgc
     2461 acagtaaacg ccattgtgtt tattatggac ccgcgacatc cagaatatga atctgacact
     2521 cgcgctcagg tcatcgcgcc gttgattgcg gcggcgagcg gtccgctggg aaccaacgca
     2581 caatacctgt tttcactgga acaggagctt atcaaactgg gaatgcagga cgatgggctg
     2641 aatgatttgc tggtatcggt aaaaaaactg ctggcggaga attatccgga tggtgtgtta
     2701 cggccgggat tcgcctgagt aaacttcccg catagtgggg cgtcagacgc cccctcaaac
     2761 attaaaatgt gagcacttta tcggctgaca gcgtccattg cgccagttcc acaagagtac
     2821 cgatttccac cccatcaatc agaggaagtg tactaatccc gcgcccgtcg gtacaggttt
     2881 tgcacaattt caccggtaca ttctgagcgg taaggatctc cagcatttgc tgaatgttgt
     2941 agccttcccc tggtttttgc ccgcgcaacc cggctgtgac cgcatcagac atgaggaaca
     3001 gacgcagatc cagattgctc tcctgctctc gtaacgcaat ggccagccgc aagctgttaa
     3061 acaaggattc gctcccgtaa ggtgcgccat tggcaacgat cacgattttt tgcattattt
     3121 actcctgtat tcagggaatt agacactcat cttctatctt actgcttctg cagcgtctga
     3181 ccaatcggtc acatttttaa ggattttcct gaaagcgcga gaaaatacga caaaagttgc
     3241 cagtaatcgt tattctttaa ggctatggtt ttcattatta ccggaagtta ccgacgtttt
     3301 gagccgtttc gttcctcgca ttatcccgtt ttatttactc ttgcttgtag caggcggtac
     3361 agctaacgca caatctacct tcgagcaaaa agcggcaaat ccctttgata ataacaatga
     3421 tggtctgccg gatttaggca tggctcccga aaatcatgat ggggaaaaac actttgctga
     3481 aattgtgaaa gatttcggcg aaaccagtat gaatgataac gggctggata ctggcgagca
     3541 ggcaaaagct ttcgcattgg gaaaagtccg cgacgcgctt agtcaacagg ttaatcagca
     3601 cgtagagtcc tggctatcac cgtggggaaa tgccagtgtt gacgtcaaag tggataacga
     3661 aggacatttc accggcagtc gtggaagctg gtttgtgccg ttacaagata atgatcgtta
     3721 tttcacctgg agccagcttg gtcttactca gcaggataat gggttggtga gcaatgtggg
     3781 cgttgggcaa cgctgggcgc gcggcaactg gctggtgggt tataacactt tttatgacaa
     3841 cttgctggac gaaaatcttc agcgagcggg ctttggtgcc gaagcgtggg gcgaatattt
     3901 gcgattattg gcaaactttt atcagccgtt tgctgcatgg catgaacaga cagccacgca
     3961 ggaacaacgg atggcgcgcg ggtacgacct gacagctcgg atgcgcatgc cgttctatca
     4021 acacctcaat accagtgtca gcctagaaca gtattttggt gatcgtgttg atttgtttaa
     4081 ctctggtacg ggttatcaca atcccgtcgc gttgagtctg ggattaaatt acacccctgt
     4141 gccattagtc actgtgacgg cccagcataa acagggtgaa agtggcgaga atcaaaataa
     4201 cctcgggctg aatcttaatt accgcgttgg tgtaccgctc aaaaaacaac tttctgcggg
     4261 cgaggttgcc gaaagtcagt cgttacgtgg tagtcgctat gacaatccgc agcgaaataa
     4321 tctaccgact cttgagtacc gacagcgaaa aacgttaacg gtgtttctgg cgacaccgcc
     4381 gtgggatcta aaacctggcg aaacagtgcc gctgaaatta caaatccgca gtcgttacgg
     4441 tattcggcaa ctgatttggc agggcgatac gcagatatta agtttgacgc cgggcgcaca
     4501 agccaacagc gcggagggct ggacgctgat catgcctgac tggcagaacg gggaaggggc
     4561 aagcaatcac tggcgattgt cggtggtggt ggaatataac caggggcagc gtgtctcctc
     4621 caatgagatc acgctaacgc ttgtcgaacc gttcgatgca ttgtcaaacg acgaactgcg
     4681 ctgggaaccg taatcagaaa atgcgctcct gatgcaccca taccgctgct tccacgcgag
     4741 acttgagctt cattttcttc agcatgtgct tgacgtgcac ttttactgtg ctttcggtga
     4801 tatccaggcg gcgggcaatc atcttgttcg gcaaaccctg ggcaatcagc ttgagaatat
     4861 cgcgctcgcg tggggttaac tggttaacat cgcgctcagt agtggcacgg ttagcgcgca
     4921 agctggcggc cagaacaggc gttaatgctt cgcttaatac catttcgcca gcagctgcct
     4981 gatgcaatgc tttcagcaga tcttccggtt ccatatcttt taacagataa ccatccgcgc
     5041 cgcgtttcag tgcggtgacc acatcttctt catggttaga gacgctgaat accacaatgc
     5101 gccctgagag ggacttttcg cgcagtttat ccagcgtttc cagaccgttc atgccgggca
     5161 tattgagatc taacaggatc agatcgggat caagagactc cgccagttca ataccctgtt
     5221 cgccattact cgcttcgcca accacggtga tatctggtgc catactgata agctgtttta
     5281 cgccagttcg cagcatcggg tgatcgtcga tcagcaggat agtagccggt tcctgattac
     5341 tcatgggtat ctccttggac gtctgtgaaa gttttttcgg gaataaaggt gaccaccact
     5401 tcggtgccac ctgattcacg acggcggacg cggcaatcgc ctcgtaaact ttgcgcacgg
     5461 tcgcgcatta ttatcatgcc gtagtgattg ctgcggatgg cattttcagg cacgccgcag
     5521 ccgttatctt ggacggtcag tttgacctga ttatcgtttt gcgccaccgt caccacgacc
     5581 tcactcgctt gcgaatgttt gagggcgtta cttaatgcct cacgggcaat ttgcaacaag
     5641 tggattgcct gatgtgaagg caccagacga ggcggcaatt gataatccag cttcaccggg
     5701 aagccaaatt tggcgctgta ctcttcgcaa ctcgcctcca gcgccggacg taatccaggc
     5761 tcggtgagct gcaagcggaa tgtggtgagc aattcacgca actgcgccca ggatgcattc
     5821 agttcgttac ggatctgact taacagttcg cggctgcttg ctggcagcgc atcgccctgc
     5881 atctgtaaac aactcacctg catcttcatg caagagagag attgggcaat agaatcatgc
     5941 agttcgcgcg caatggtggc acgctcttcc atcacgatca actgttgctg acgttcctga
     6001 tggcgatcca gcgccagcgt ggcggtgagt tgttcaacca gggtatccac cagttgttgt
     6061 tgatcatggc taagatgacg cccctgcggc agggtcgcca gcaaaatacc gtactgcgta
     6121 tgagagtcag ccagccgcca cttcagggtt gtgccgcgat caccaacggg taatacgccg
     6181 cgcgggcaga gctggcagcc tttatcatca caagtcatat ctggctggca ggtaaactcc
     6241 tgatgattct cttcatcatc agtgtcatac acccgcagtt cgatatcacg tagcagggtt
     6301 aaattctgta agccgttgag tacaggtgac aggcgttcac acagcggggc gcgggaatgc
     6361 aaacggcggt tagcctgcca taaaaaagag aggatctgat ttttatgctc cagcccggcg
     6421 gttttctcct gaacccgctg ctcaagtacg gcataacttt cggccagttc tgcagacata
     6481 ttgttcaatg cagttccaag catcgccatt tcgttgcgcc cgctgatgtt cgcgcgttgg
     6541 gtaaaatcgc gatgactgac ggcactcgcc attgccagca gttgccgcca cggttgtagc
     6601 agtcgcgccc gcaaccagat aatagtgaac accagtaaaa gtgccataaa taccgccatt
     6661 acccgatgga ccagtaccac tgtctcaatg cgcatttccg tggtgcggtc aaaaccagat
     6721 accagctgat caagcccggc aacaaactgg ctgacatccg ctgacaccgt ttcgcggttt
     6781 tgtgcacgca tcagtgcagg gatcagttca ttacgccagt aatcttgtaa accctgtaat
     6841 tgcgccagtt gttcgtctcg ttctgctgct cgagttaact cggcgctaaa tgccgtttgt
     6901 tccatctctt taattaaggg cttgtctttc tcacttaatg gcactgccgc caacagacgg
     6961 taactttgca tgcgcagcga tcccgctttg ttgatcgcat gggcgctgcc ctgaacgcct
     7021 tgcaccagcc agccagaaac cgccatccct gccagtccaa tagcagtaga aagcaacaca
     7081 ataagcgcaa cctgattaac cagggtgagc ggagagagac aacgtttaag catgtaaacc
     7141 tcttccttca ggctttaaat gagcaataac cttaatgaat gtgacgatac attctggaat
     7201 ggcagtattc tcggctattg gctgaagtat acccataccc ggaaagagtt actccttatt
     7261 tgccgtgtgg ttagtcgctt tacatcggta agggtaggga ttttacagca ccgtgaaaaa
     7321 tctcataatt tttatgaaat cactgtactc actatgggta atgataaata tcaatgatag
     7381 ataaagttat cttatcgttt gatttacatc aaattgcctt tagctacaga cactaaggtg
     7441 gcagacatcg aaacgagtat cagaggtgtc tatgagtcac tcatccgccc ccgaaagggc
     7501 tactggagct gtcattacag attggcgacc ggaagatcct gtgttctggc aacaacgcgg
     7561 tcaacgtatt gccagccgta acctgtggat ttccgttccc tgtctgctgc tggcgttttg
     7621 cgtatggatg ttgttcagcg ctgttgcggt gaacttaccg aaagtcggtt ttaattttac
     7681 gaccgatcag ctatttatgt tgactgcgct gccttcggtt tctggcgcgt tattacgtgt
     7741 tccatactcc tttatggttc ctatcttcgg tggtcgtcgc tggacggcgt tcagcaccgg
     7801 tattctgatt attccttgcg tctggctggg ttttgccgtg caggatacct ccacgcctta
     7861 tagcgtcttc atcatcatct ctctgctatg cggctttgct ggcgcgaact tcgcatccag
     7921 tatggcaaac atcagcttct tctttccgaa acagaagcag ggtggcgcgc tgggtctgaa
     7981 tggtggtctg ggaaacatgg gcgtcagcgt catgcagttg gttgctccgc tggtggtatc
     8041 actgtcgatt ttcgcagtat ttggtagcca gggcgtcaaa cagccggatg ggactgagct
     8101 gtatctggcg aatgcgtcct ggatatgggt gccgttcctt gccatcttca ccattgcggc
     8161 gtggtttggc atgaacgatc ttgctacctc gaaagcctcc atcaaggagc agttgccggt
     8221 actcaaacgg ggtcatctgt ggattatgag cctgctgtat ctggcaacct tcggctcctt
     8281 catcggcttc tccgcgggct ttgcaatgct gtcaaaaacg cagttcccgg atgttcagat
     8341 tctgcaatac gctttcttcg ggccgtttat tggtgcgctg gcgcgttctg caggtggtgc
     8401 attatctgac cgtctgggcg gaactcgtgt cacgctggtg aactttattc tgatggcgat
     8461 tttcagcggc ctgctgttcc tgaccttacc gactgacggg cagggcggaa gcttcatggc
     8521 gttcttcgcg gtcttcctgg cgctgttcct gacagctggg ctgggtagtg gttccacttt
     8581 ccagatgatt tcagtgatct tccgtaaact gacaatggat cgcgtgaaag cagaaggggg
     8641 ttctgacgaa cgtgcgatgc gtgaagcggc aaccgacacg gcggcggcgc tgggtttcat
     8701 ctctgcgatt ggcgcgattg gtggcttctt tatcccgaaa gcgtttggta gctcgctggc
     8761 attaacgggt tcgccagtcg gcgcaatgaa ggtatttttg attttctata tcgcctgcgt
     8821 agtgattacc tgggcggtat atggtcggca ttctaaaaaa taaaccgtta ctcgtcatac
     8881 tccgggttac atgtgctgcg gctgcgttca ttcaccccag tcacttactt tagtaagctc
     8941 ctgggattca ttcacttgcc gccttcctgt aaaccgaatt atatagagta aaatatttga
     9001 ttatcctttg cgcggcatga tgtcgcgctt tttttaagtg ttatttagtt acaacatact
     9061 aatgttatat ggtttatttc gccggatttc attaagagcc attaatatgt tacccatggg
     9121 gaatactcct taatacccat ctgcataaaa atcttaatag tttaaataac tacaggtata
     9181 aaacgtctta atttacagtc tgttatgtgg tggctgttga ttatcctaaa ggggtatctt
     9241 aggaatttac tttatttttc atccccatca ctcttgatcg ttatcaattc ccacgctgtt
     9301 tcagagcgtt accttgccct taaacattag caatgtcgat ttatcagagg gccgacaggc
     9361 tcccacagga gaaaaccgat gagtaaattc ctggaccggt ttcgctactt caaacagaag
     9421 ggtgaaacct ttgccgatgg gcatggccag cttctcaata ccaaccgtga ctgggaggat
     9481 ggatatcgcc agcgttggca gcatgacaaa atcgtccgct ctacccacgg ggtaaactgc
     9541 accggctcct gcagctggaa aatctacgtc aaaaacggtc tggtcacctg ggaaacccag
     9601 cagactgact atccgcgtac ccgtccggat ctgccaaacc atgaacctcg cggctgcccg
     9661 cgcggtgcca gctactcctg gtatctttac agtgccaacc gcctgaaata cccgatgatg
     9721 cgcaaacgcc tgatgaaaat gtggcgtgaa gcgaaggcgc tgcatagcga tccggttgag
     9781 gcatgggctt ctatcattga agacgccgat aaagcgaaaa gctttaagca ggcgcgtgga
     9841 cgcggtggat ttgttcgttc ttcctggcag gaggtgaacg aactgatcgc cgcatctaac
     9901 gtttacacca tcaaaaacta cggcccggac cgtgttgctg gtttctcgcc aattccggca
     9961 atgtcgatgg tttcttacgc atcgggtgca cgctatctct cgctgattgg cggtacttgc
    10021 ttaagcttct acgactggta ctgcgacctg cctcctgcgt ctccgcaaac ctggggcgag
    10081 caaactgacg taccggaatc tgctgactgg tataactcca gctacatcat cgcctggggt
    10141 tcaaacgtgc cgcagacgcg taccccggat gctcacttct ttactgaagt gcgttacaaa
    10201 ggcaccaaaa ctgttgccgt cacaccagac tacgctgaaa tcgccaaact gtgcgatctg
    10261 tggctggcac cgaaacaggg caccgatgcg gcaatggcgc tggcgatggg ccacgtaatg
    10321 ctgcgtgaat tccacctcga caacccaagc cagtatttca ccgactatgt gcgtcgctac
    10381 accgacatgc cgatgctggt gatgctggaa gaacgcgacg gttactacgc tgcaggtcgt
    10441 atgctgcgcg ctgctgatct ggttgatgcg ctgggccagg aaaacaatcc ggaatggaaa
    10501 actgtcgcct ttaataccaa tggcgaaatg gttgcgccga acggttctat tggcttccgc
    10561 tggggcgaga agggcaaatg gaatcttgag cagcgcgacg gcaaaactgg cgaagaaacc
    10621 gagctgcaac tgagcctgct gggtagccaa gatgagatcg ctgaggtagg cttcccgtac
    10681 tttggtggcg acggcacgga acacttcaac aaagtggaac tggaaaacgt gctgctgcac
    10741 aaactgccgg tgaaacgcct gcaactggct gatggcagca ccgccctggt gaccaccgtt
    10801 tatgatctga cgctggcaaa ctacggtctg gaacgtggcc tgaacgacgt taactgtgca
    10861 accagctatg acgatgtgaa agcttatacc ccggcctggg ccgagcagat taccggcgtt
    10921 tctcgcagcc agattattcg catcgcccgt gaatttgccg ataacgctga taaaacgcac
    10981 ggtcgttcga tgattatcgt cggtgcgggg ctgaaccact ggtatcacct cgatatgaac
    11041 tatcgtggtc tgatcaacat gctgattttc tgcggctgtg tcggtcagag cgggggcggc
    11101 tgggcgcact atgtaggtca ggaaaaactg cgtccgcaaa ccggctggca gccgctggcg
    11161 tttgcccttg actggcagcg tccggcgcgt cacatgaaca gcacttctta tttctataac
    11221 cactccagcc agtggcgtta tgaaaccgtc acggcggaag agttgctgtc accgatggcg
    11281 gacaaatccc gctataccgg acacttgatc gactttaacg tccgtgcgga acgcatgggc
    11341 tggctgccgt ctgcaccgca gttaggcact aacccgctga ctatcgctgg cgaagcggaa
    11401 aaagccggga tgaatccggt ggactatacg gtgaaatccc tgaaagaggg ttccatccgt
    11461 tttgcggcag aacaaccaga aaacggtaaa aaccacccgc gcaacctgtt catctggcgt
    11521 tctaacctgc tcggttcttc cggtaaaggt catgagttta tgctcaagta cctgctgggg
    11581 acggagcacg gtatccaggg taaagatctg gggcaacagg gcggcgtgaa gccggaagaa
    11641 gtggactggc aggacaatgg tctggaaggc aagctggatc tggtggttac gctggacttc
    11701 cgtctgtcga gcacctgtct ctattccgac atcattttgc cgacggcgac ctggtacgaa
    11761 aaagacgaca tgaatacttc ggatatgcat ccgtttattc acccgctgtc tgcggcggtc
    11821 gatccggcct gggaagcgaa aagcgactgg gaaatctaca aagccatcgc gaagaaattc
    11881 tccgaagtgt gcgtcggcca tctgggtaaa gaaaccgaca tcgtcacgct gcctatccag
    11941 catgactctg ccgctgaact ggcgcagccg ctggatgtga aagactggaa aaaaggcgag
    12001 tgcgacctga tcccaggtaa aaccgcgcca cacattatgg tcgtagagcg cgcttatccg
    12061 gcgacttacg aacgctttac ctctatcggc ccgctgatgg agaaaatcgg taatggcggt
    12121 aaagggattg cctggaacac ccagagcgag atggatctgc tgcgtaagct caactacacc
    12181 aaagcggaag gtccggcgaa aggccagccg atgctgaaca ccgcaattga tgcggcagag
    12241 atgatcctga cactggcacc ggaaaccaac ggtcaggtag ccgtgaaagc ctgggctgcc
    12301 ctgagcgaat ttaccggtcg tgaccatacg catctggcgc tgaataaaga agacgagaag
    12361 atccgcttcc gcgatattca ggcacagccg cgcaaaatta tctccagccc gacctggtct
    12421 ggtctggaag atgaacacgt ttcttacaac gccggttaca ccaacgttca cgagctgatc
    12481 ccatggcgta cgctctctgg tcgtcagcaa ctgtatcagg atcaccagtg gatgcgtgat
    12541 ttcggtgaaa gcctgctggt ttatcgtccg ccgatcgaca cccgttcggt gaaagaagtg
    12601 ataggccaga aatccaacgg caacccggaa aaagcgctca acttcctgac gccgcaccag
    12661 aagtggggga tccactccac ctacagcgac aaccagttga tgctgacctt aggtcgcggt
    12721 ggcccggtgg tctggctgag cgaagccgat gccaaagagc tgggcattgc cgataacgac
    12781 tggattgaag tttacaacag caacggtgcg ctgactgccc gtgcggttgt cagccagcgt
    12841 gttccggcag ggatgaccat gatgtaccac gcgcaggaac gtatcgttaa cctgccgggt
    12901 tcggaaatta ctggccagcg tggggggatc cacaactcgg tcacccgtat cacgccgaaa
    12961 ccgacgcata tgattggtgg ctatgcccat ctggcatacg gctttaacta ctacggcacc
    13021 gtaggttcta accgcgatga gtttgttgta gtgcgtaaga tgaagaacat tgactggtta
    13081 gatggcgaag gcaatgacta ggtacaggag agcgtaaaat gaaaattcgt tcacaagtcg
    13141 gcatggtgct gaatctcgat aagtgcatcg gctgccacac ctgttcagtt acctgtaaaa
    13201 acgtctggac cagccgtgaa ggcgtggagt acgcgtggtt caacaacgtg gaaaccaagc
    13261 cgggtcaggg cttcccgact gactgggaaa accaggaaaa atacaaaggc ggctggatcc
    13321 gtaaaatcaa cggcaaactg cagccgcgca tgggtaaccg tgccatgttg ctgggtaaaa
    13381 tcttcgctaa cccgcatctg ccggggatcg acgattatta cgagccgttc gattttgact
    13441 atcagaacct gcataccgcg ccggaaggca gcaaatcgca gccgattgcc cgtccccgtt
    13501 cgctgattac cggggaacgg atggcgaaaa tcgaaaaagg gccgaactgg gaagatgacc
    13561 tgggtggtga gtttgacaaa ctggcgaaag acaagaactt cgacaacatc cagaaggcga
    13621 tgtatagcca gttcgaaaac acctttatga tgtatttgcc gcgcctgtgc gaacactgcc
    13681 tgaacccggc atgtgtggcg acctgcccga gcggtgcgat ttacaagcgt gaagaagatg
    13741 gcatcgtcct gatcgaccag gataaatgcc gtggctggcg tatgtgcatc accggatgcc
    13801 cgtacaaaaa aatctacttc aactggaaga gcggtaagtc tgagaagtgc atcttctgct
    13861 atccgcgtat tgaagcgggt cagccgaccg tgtgctcaga aacctgtgtc ggtcgtatcc
    13921 gttatcttgg cgtgctgctg tacgatgccg acgcgattga acgtgcagcc agcaccgaga
    13981 acgagaaaga tctttaccag cgtcagctgg aggtgttcct cgatccgaac gatccgaaag
    14041 tcatcgagca ggcgattaaa gacggtattc cgctgagcgt tattgaagcc gcacagcagt
    14101 cgccggttta taaaatggca atggaatgga aactggcgct gccgctgcat ccagaatatc
    14161 gcacactgcc gatggtctgg tacgtgccgc ctctgtctcc gattcagtct gcagcagacg
    14221 cgggtgagct gggtagcaac ggcattctgc cagacgtcga aagcttgcgt attccggtac
    14281 agtatctggc gaatctgctg accgccggtg ataccaaacc ggtactgcgc gcactgaaac
    14341 gtatgctggc gatgcgtcat tacaaacgtg ctgaaaccgt tgacggtaaa gttgataccc
    14401 gtgcgctgga agaggtcggt ctgaccgaag cccaggcaca ggagatgtac cgttatctgg
    14461 ctattgctaa ctacgaagat cgctttgtgg tgccgagtag tcatcgtgaa ctggcacggg
    14521 aagccttccc ggagaaaaat ggctgcggct ttacctttgg tgatggctgc cacggctcag
    14581 ataccaaatt caatctgttc aacagccgtc gtatcgatgc catcgatgtg accagcaaaa
    14641 cggagccgca tccatgatcg aactcgtgat tgtatcgcgt ctccttgaat atccggatgc
    14701 tgccttatgg cagcatcaac aagagatgtt tgaggtgatt gctgcgtcga aaaatctgtc
    14761 gaaagaagat gcccatgcgc tgggcatttt cctgcgcgat ttaacggcga tggatccgct
    14821 cgatgcccag gcccagtaca gcgaactgtt cgaccgtggc cgcgccacgt cactgttgct
    14881 atttgaacat gttcacggtg aatcccgtga ccgcggccag gcgatggtgg acctgctggc
    14941 gcagtacgag cagcacggct tgcagttaaa cagccgcgaa ttaccggacc atctgccgct
    15001 gtatctggag tacctgtcgc agctgccgca aagcgaagcc gtggaaggtt tgaaagatat
    15061 cgcgccgatt ctggcattgc tgagcgcgcg tctgcaacag cgtgaaagcc gttatgccgt
    15121 gatgtttgat ctgctgctga aattggccaa taccgctatc gacagcgaca aagtggcgga
    15181 aaaaattgcc gacgaagcgc gcgatgatac gccgcaggcg ctggatgctg tctgggaaga
    15241 agagcaggtt aaattctttg ctgacaaagg ctgcggtgat tcagcaatca ctgctcatca
    15301 gcgtcgcttt gccggtgccg tcgcgccgca atatctgaat atcaccaccg gaggacagca
    15361 ctaatgcaat tcctgaatat gttcttcttt gatatctacc cgtacatagc cggggcggtc
    15421 ttcctgattg gtagctggct gcgttatgac tacgggcagt acacctggcg cgcggcgtcc
    15481 agccagatgc tggatcgcaa agggatgaac ctggcgtcga acctgttcca tatcggtatt
    15541 ctggggattt ttgctggtca cttcttcggt atgctgacgc cgcactggat gtatgaagcc
    15601 tggctgccga ttgaagtgaa acagaaaatg gcgatgtttg ctggtggtgc cagcggcgtg
    15661 ctgtgtctga ttggcggcgt gctgttgctg aagcgtcgtc tgttcagccc acgcgtgcgt
    15721 gcaaccacta ccggagcgga tatcctgatc ctgtcgctgc tcgttatcca gtgcgcgctg
    15781 ggcctgttga ccattccgtt ctccgctcag catatggacg gtagcgagat gatgaaactg
    15841 gttggctggg cgcagtcggt ggtgaccttc cacggtggcg cttctcaaca cctcgatggt
    15901 gtggcgttta tcttccgtct gcacctggtg ctggggatga cgttattcct gctgttcccg
    15961 ttctcgcgtc tggtacacat ctggagcgta ccggtggagt atctgacacg taagtaccag
    16021 ctggtgcgcg ctcgtcacta aacgaatttt agttcacata gaccctgctt cggcggggtt
    16081 ttttatgggc acggtgcggg gtgagttgtc ggatgcgctt cgcttatccg acctacaggg
    16141 gaggatattg taggcccggt aaacgtggtg ccatcgggca aaattagctc ggagtaacag
    16201 gttttgatta ttcaaggaag cgatggtggt gggggaagga ttactcagcg ctgcgcgctt
    16261 cgcccttcgg gtcgttgcct gcggcaacgc tctctcgctg gcgctcaagt cgaaccttgg
    16321 tcgaagcttc tcatccttcc ccgcttgggc agaatattta attgcggatt cgtttgagag
    16381 tttacggact tttgaaaagt gatggtggtg ggggaaggat tcgaaccttc gaagtcgatg
    16441 acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccacg gggtaatgct
    16501 ttttactggc ctgctccctt atcgggaagc ggggcgcatc atatcaaatg acgcgccgct
    16561 gtaaagtgtt acgttgagaa aaatgaactg gttgcgtaat tttcatccgt aacggattaa
    16621 aggtaaccag ttatttttgc tggcgattaa agaataatcg tccgattacc gtaaacaaag
    16681 acgcgctgtg ccagtacttt gtatagcgca cgacttaaga cgtttttctc gacgtcacga
    16741 cctgcgcgca tcatatcttc agctgtgtag gtatgatcga catgaataac gtcctgcatg
    16801 atgattgggc cttcgtccag attgtcattc acatagtgag cggttgcgcc aataatcttc
    16861 acaccacgtt cataggcctg gtgataagga cgtgcgccga taaacgcagg caggaatgaa
    16921 tggtgaatat tgatgatttt attcgggaag cgtgccacaa attccggcgt taatacccgc
    16981 atatacttcg ccagcaccac atagtcaggt ttataagcat caatggcgtc tgccatcttt
    17041 tgatcgtgct cgttgcggct taacccttca tggtttacca gctcaaacgg aatatcaaaa
    17101 cgctcaacca gagaacgtaa agtatcgtgg ttaccaataa ctgccgcgat ttcgacatcc
    17161 aggccgccgt aattggcttt catcaacaaa tcgccaaggc aatgcgcttc tttagtgacc
    17221 agaatcacta tccggcgacg accggcagga ttcagctcac gcacggagcc ttctggcaat
    17281 gcgctatcga gatccgccag cagggtggaa tcattaaaaa tcccttccag ttccgtacgc
    17341 ataaaaaagc gcccggtacg gtgatcaaca aattcattgt tctgtacgat atttaactcg
    17401 tgcttgtagc aaatattggt aatacgtgcg atcagacctt tttggtccgg acaaatagta
    17461 cgcagaactt tacgttggag tgaatgcatt gctggaaaaa ccttgttgag agtgtttgct
    17521 aaaccgtacc gtcaaccatt attggccgca gcacttttta aattttttac ctgaaccaca
    17581 agggcaggga tcgttgcgac caaactgcgg acgtgtaccg tcaatatagt accattgacc
    17641 gttttctttc aaaaatcgcg aacgttcaat tattgcacct gttttgccac cttcggtaaa
    17701 tcgagcaaca aaactgacaa atccgatatt gtcggcgtcc tgccaacaat gttcaaatac
    17761 ggtcaatccc aaccattcag tatgcgcaaa tccggccatc aattcggcac gtaacgccgc
    17821 tgctccacaa gagggatgcc aggtctttat taaataatct gcgtcttgca tcacaaaagc
    17881 gcagtaacgc gaacgcatga gatgttctgg atcaggtgca accttttcac cagacacata
    17941 agggtggcaa cataggctat actcgacagc actaccacag ggacaaagct gagacacaaa
    18001 taatctccct ggaaacaata acggcgtatt aaccgcctga gtagcactat gttaaccgag
    18061 cagtagcgat gtggctacga ttgcattcca ggggaatctt gcgggaataa tgagaaagat
    18121 aaaaataggg ctggcgctgg gatctggcgc ggcgagaggt tggtcgcata ttggcgttat
    18181 taatgcgcta aaaaaagtgg gtattgaaat tgatatcgtt gcaggatgtt caattggttc
    18241 gctggtgggc gctgcctatg catgcgatcg attatctgcg ctggaagatt gggtgacctc
    18301 tttcagttat tgggatgttt tacgcctgat ggatctctcc tggcagcgcg gtgggttact
    18361 gcgcggcgag cgtgtcttca atcaatgccg cgaaataatg ccggaaacag agatcgaaaa
    18421 ttgttcccgt cgctttgcgg ctgttgccac caatttaagt acgggacgtg aattatggtt
    18481 tactgaaggc gatctccatc ttgctattcg cgcatcatgc agtattccag gactcatggc
    18541 acctgttgca cataacggct actggctggt tgatggagca gtagttaacc caattcctat
    18601 ttccctcacg cgtgcattgg gggctgatat tgtgatagcg gtcgacctgc agcacgatgc
    18661 tcatttgatg caacaagatt tgctctcctt taatgtcagt gaagaaaata gcgagaatgg
    18721 tgaatctttg ccgtggcatg cgcgtctgaa agaaaggtta ggcagtataa caacacgtcg
    18781 ggcggtgaca gcgccaacgg caacagagat tatgaccact tctatccagg tgctggagaa
    18841 ccgccttaaa aggaaccgca tggcaggtga tccgcccgat attctgattc aacctgtttg
    18901 cccgcaaata tctacgcttg atttccatcg cgcgcacgct gccattgcgg ccggacagct
    18961 ggcagtggaa aggaaaatgg acgaactttt gccgttggta cgcaccaaca tttgaccaga
    19021 atttttatct acacttaagt taattctgac aggcgcaggt ggcaatagca tgccactatt
    19081 gagtaaagcc agtcagggga gagaacatga cgcagccatt ggtcggaaaa cagattctca
    19141 ttgttgaaga tgagcaggta tttcgctcgc ttctggattc atggttttcc tcattgggag
    19201 cgacaacggt actggcggct gatggggtgg atgcccttga gttgctggga ggtttcactc
    19261 cagacctgat gatatgtgat atcgcgatgc cacgaatgaa cgggcttaaa ctgctggagc
    19321 atatacgtaa cagaggcgac cagaccccag ttctggtgat atctgccact gaaaatatgg
    19381 cagatattgc caaagcgtta cgtctgggcg ttgaagatgt tttgctgaaa ccagttaaag
    19441 atctgaatcg cttgcgcgag atggtttttg cctgtctcta tcccagcatg tttaattcgc
    19501 gcgttgagga agaggaaagg ctttttcgcg attgggatgc aacggttgat aaccctgccg
    19561 cagcggcgaa attattacag gaactacaac cgccggttca gcaggtgatt tcccattgcc
    19621 gggttaatta tcgtcaattg gttgccgcgg acaaacccgg cctggtgctt gatattgccg
    19681 cactttcgga aaacgatctg gcattttatt gccttgatgt cacccgagct ggacataatg
    19741 gcgtacttgc tgccttgtta ttacgcgcat tgtttaacgg attattacag gaacagcttg
    19801 cacaccaaaa tcaacggttg ccagagctgg gcgcgttatt gaagcaggta aaccatttac
    19861 tccgtcaggc caatctgccg gggcagtttc cgctattagt tggctattat catcgcgaac
    19921 tgaaaaatct cattctggtt tctgcgggtc tgaatgcgac gttaaatacc ggcgaacacc
    19981 aggtgcaaat cagtaatggt gttccgttag gcactttagg taacgcttat ttgaatcaat
    20041 tgagccagcg atgcgatgcc tggcaatgcc aaatatgggg aaccggtggt cgactgcgct
    20101 tgatgttgtc tgcagaatga gcaaacgata acgcggacta aatttgcatt acctgctaat
    20161 gtcggctggt ggtactatcg tcgccattcg tataagtaat tgtcttaatt atgctaactc
    20221 gcctcctttt cagaacttag ccccttcggg gtgctgatat actgggatgc gatacagaaa
    20281 tatgaacacg ttcaaaacac gaacagtcca ggagaattta aatggctgcc attaatacga
    20341 aagtcaaaaa agccgttatc cccgttgcgg gattaggaac caggatgttg ccggcgacga
    20401 aagccatccc gaaagagatg ctgccacttg tcgataagcc attaattcaa tacgtcgtga
    20461 atgaatgtat tgcggctggc attactgaaa ttgtgctggt tacgcactca tctaaaaact
    20521 cgattgaaaa ccactttgat accagttttg aactggaagc aatgctggaa aaacgtgtaa
    20581 aacgtcaact gcttgatgaa gtgcagtcta tttgtccgcc gcacgtgact attatgcaag
    20641 ttcgtcaggg gctggcgaaa ggcctgggac acgcggtatt gtgtgctcac ccggtagtgg
    20701 gtgatgaacc ggtagctgtt attttgcctg atgttattct ggatgaatat gaatccgatt
    20761 tgtcacagga taacctggca gagatgatcc gccgctttga tgaaacgggt catagccaga
    20821 tcatggttga acaggttgct gatgtgaccg catatggcgt tgtggattgc aaaggcgttg
    20881 aattagcgcc gggtgaaagt gtaccgatgg ttggtgtggt agaaaaaccg aaagcggatg
    20941 ttgcgccgtc taatctcgct attgtgggtc gttacgtact tagcgcggat atttggccgt
    21001 tgctggcaaa aacccctccg ggagctggtg atgaaattca gctcaccgac gcaattgata
    21061 tgctgatcga aaaagaaacg gtggaagcct atcatatgaa agggaagagc catgactgcg
    21121 gtaataaatt aggttacatg caggccttcg ttgaatacgg tattcgtcat aacacccttg
    21181 gcacggaatt taaagcctgg cttgaagaag agatgggcat taagaagtaa catccatatc
    21241 ggtgttatcc acgaaacggc gttgaacaat cgacgccgtt tttttatagc ttattcttat
    21301 taaattgtct taaaccggac aataaaaaat cccgccgatg gcgggatttt tagcaattgc
    21361 aatctacaaa agattattgc ttgatcagga aatcgtcgag ggatttacct tgctcatcca
    21421 ttgctttttt gattacagct ggagtacggc cctggccagt ccaggtttta gtttcgccgt
    21481 tttcgtcaac atagctatat tttgccggac gctgagcacg tttagctttg gtgccggatt
    21541 taacggcagc aaggctattc agcagttcgt tcgggtcaat accgtcagcg atcagcattt
    21601 cgcgatattg ctgcagttta cgagtgcgct cttcaacttc agcagcagcc gcgctttctt
    21661 cttcgcgacg ttcgttaaca acaacttcta atttttccag catttcttcc agcgtttcaa
    21721 gtgtacattc tcttgcctgc gcacgaagag tacggatgtt gttcagaatt ttaagtgctt
    21781 cgctcattgt agtaatctca aacttatatt ggggtggttt gttgaggtaa taatagagcc
    21841 ttaaattcag ttgtgcaata gccaggaatg taaggaattc aaaattgttc tttattttgt
    21901 gccgccaata aatatctttt cataaaatta gccagaaaag acgcggcata tagccctatt
    21961 tacaccgatg atttcgcagc acgtgaggtt aaaacttcct gattcatgtc acattttatg
    22021 gggagattat cgtaggctga cggcctttca gtcttctgta ttagttgtgt ttacgagaat
    22081 tccctattaa gcgaatgatg aaaagtagaa cagtcgcaat aagagcatgg acttagtatt
    22141 gcactatctc ctggaggtca acagagggct attacttgcg caacaggtta aagattgtga
    22201 atagttacca gcagtcattt acccgcttat aacaagcgag gcagttgtaa tgatagctca
    22261 gaaggattat gcaaggcttc gtaagggaga acgcatatac ccacttctgt gcatactgtt
    22321 gagctgaaaa actgacgaat tatgataaac tccagccaac tttatttcat atcattgagg
    22381 gcctgtggct gatggcacag ctatatttct actattccgc aatgaatgcg ggtaagtcta
    22441 cagtattgtt gcaatcttca tacaattacc aagaacgcgg catgcgcact gtcgtatata
    22501 cggcagaaat tgatgatcgc tttggtgccg ggaaagtcag ttcgcgtata ggtttgtcat
    22561 cgcctgcaaa attatttaac caaaattcat cattatttga tgagattcgt gcggaacatg
    22621 aacagcaggc aattcattgc gtactggttg atgaatgcca gtttttaacc agacaacaag
    22681 tatatgaatt atcggaggtt gtcgatcaac tcgatatacc cgtactttgt tatggtttac
    22741 gtaccgattt tcgaggtgaa ttatttattg gcagccaata cttactggca tggtccgaca
    22801 aactggttga attaaaaacc atctgttttt gtggccgtaa agcaagcatg gtgctgcgtc
    22861 ttgatcaagc aggcagacct tataacgaag gtgagcaggt ggtaattggt ggtaatgaac
    22921 gatacgtttc tgtatgccgt aaacactata aagaggcgtt acaagtcggc tcattaacgg
    22981 ctattcagga aaggcatcgc cacgattaat aagaatttct ttactgacag ggtgagcagg
    23041 gcacttttat cctgtcagtt cgttttacgc acttcttccg ggctatatac ccttctcggc
    23101 agttttttaa cgccgctata cgcctcacag ggctcttaag caccgacgtt gacttgtgac
    23161 ctgtaaagta caatatccct gtgtttaggc gttatacatc gtcgcaaata tgatgaaggc
    23221 taatgctgtc ggtttatgga aaagttgctt tgggtaaaca aaaaatacgg ccccagaagg
    23281 gcaatgccgt tcacttaaga ggagcggcac tatgtttcac aggataacgg gtttttgata
    23341 tcttaaccga cctcggcctt gatggtcggg ggcgttttgt tatgaacacc acttccagag
    23401 caccccgaag atgctccagt cgtttcggga tggtccctgg tgacgccgtg tttcccagct
    23461 ctatcatttc tgatgcgata ttcttccacg caggcagtag ccagtggcgg ttacaaccct
    23521 tctggttcag cgttagcagc aggtcttcgc tgtaaaaaag cttatcaaac aacgtaatag
    23581 agttatccgg gatggtggcg agcatggagt gggccagcac agtttcgctc tgccggtaag
    23641 gtgcggtcac ggcattcagc agaatgtgac ttcccaggtt cattaaggcc accagacgca
    23701 taaccgggta ggcgttctgc cgcttagtgg atgtgttggc agacccataa tattcacgca
    23761 gctcgggttt atcaggtgtc ctgaactgtg cgccatcaat ggcaaaaagt tgcaggccgt
    23821 gccagtcatc cttcaggtaa cgttccgcgc ccctgtctgt gcggtctggc ggaagagcca
    23881 ttccactggg gcggccccca cgcgctgacg cgcttgggtg acagcgctgc gggccagcag
    23941 gttcatcccc gcttcgccat ccgcgctcag gttcagacgg cgaacaacat cggtaattgg
    24001 ctcattgcac caccatccag ataaccatgt cccccggtaa acgacggcgg cgaacggtcg
    24061 catgagcaga aagcgtcagg cagtgttgta tccactcggt gggaaggtgt tctgcaaata
    24121 gttgtgcaga gggcggaggc ataagcggat ggtcactgaa atcgagcaga tcattgagaa
    24181 gtggcataag aaaacggctc cctgttgtgg aagccgttat agtgcctcag tttaaggatc
    24241 ggtcaactaa tccttaactg atcggcattg cccagaaggg gccgtttatg ttgccagaca
    24301 gcgctactga ttaagcggat tttttcgctt ttttctcagc tttagccgga gcagcttctt
    24361 tcttcgctgc agtttcacct tctacataat cacgaccgta gtaggtatcc agcagaatct
    24421 gtttcagctc ggagatcagc gggtaacgcg ggttagcgcc ggtgcactgg tcatcgaatg
    24481 catcttcaga cagtttatcc acgttcgcca ggaagtctgc ttcctgaacg ccagcttcac
    24541 ggatagattt cggaataccc agttcagctt tcagcgtttc cagccatgcc agcagtttct
    24601 cgatcttagc agcagtacgg tcgcccggtg cgctcagacc caggtggtca gcaatttcag
    24661 cataacgacg gcgagcctgc ggacggtcat actggctgaa tgcagtctgc ttggtcgggt
    24721 tgtcgttcgc gttgtagcga ataacgttac aaatcagcag ggcgtttgcc agaccgtgcg
    24781 gaatatggaa ctgggaaccc agtttgtgcg ccattgagtg acatacaccc aggaaggcgt
    24841 tcgcaaacgc gatacccgcg atagtcgctg cactgtgaac acgttcacgc gctaccggat
    24901 ttttagaccc ttcgtggtag gacgctggca gatattcttt cagcagtttc agtgcctgca
    24961 gagcctgacc atcagagaac tcagatgcca gtacagaaac ataagcttcc atggcgtgag
    25021 ttactgcgtc cagaccaccg aaagcacaca gggacttcgg catgtccata accaggttgg
    25081 cgtcgacaat cgccatatcc ggagtcagcg catagtctgc cagcggatat ttctgaccag
    25141 tagcgtcgtc agttacaacc gcaaacggag tgacttcaga acctgtacca gaagtggtgg
    25201 tgacagcgat cattttcgct ttcacgccca ttttcgggaa cttgtagata cgtttacgga
    25261 tatccataaa gcgcagcgcc agctcttcga agtgagtttc cggatgttcg tacataaccc
    25321 acatgatctt cgcggcgtcc atcggggaac caccacccag cgcgataatc acgtctggtt
    25381 tgaaggagtt tgccagttct gcacctttac gaacgatgct cagggtcggg tccgcttcta
    25441 cttcgaagaa gacttcagtt tcaacgcctg ctgctttcag tacggaagtg atctgatcag
    25501 cataaccatt gttgaacagg aagcggtcag tcacgatgag cgcacgtttg tggccatcag
    25561 taatcacttc atccagcgcg attggcaggg agccacggcg gaagtagata gatttcggaa
    25621 gtttgtgcca caacatgttt tcagctcgct tagcaacggt tttcttgttg atcaggtgtt
    25681 tcggaccaac gttttcagag atggagttac caccccaaga accacaaccc agagtcaggg
    25741 aaggtgcgag tttgaagtta tacaggtcac cgataccacc ctgagacgct ggggtgttaa
    25801 tcaggatacg agccgttttc attttctgac cgaagtaaga aacgcgagcc ggttggttat
    25861 cctggtcagt gtacaggcaa gaggtatgac cgataccgcc catagcaacc agtttctctg
    25921 ctttttctac cgcgtcttcg aaatctttag cgcggtacat tgccagagtc ggggacagtt
    25981 tttcatgtgc gaacggttcg ctttcatcaa caacggtcac ttcaccgatc agaatcttgg
    26041 tgttttctgg tacagagaag cctgccagtt cagcaatttt ataggctggc tgaccaacga
    26101 tagccgcgtt cagcgcaccg tttttcagga taacatcctg aacagctttc agctctttac
    26161 cctgcaacag atagccgccg tgggttgcaa aacgttcacg tacagcgtca taaacagagt
    26221 caacaacaac aacagactgt tcagaagcac agattacgcc gttgtcgaag gttttggaca
    26281 tcagtacaga tgcaactgca cgtttgatat cagcagtttc atcgataaca actggagtgt
    26341 tgcccgcgcc tacaccgata gctggtttac cggagctgta tgcggcttta accatgcccg
    26401 gaccaccagt cgcgaggatc aggttgatgt ctgggtggtg catcagtgcg ttagacagtt
    26461 caacagaagg ttgatcgatc cagccgatca gatctttcgg agcaccggca gcgatagcag
    26521 cctgcagaac gatatcagcc gctttgttgg tggcatcttt tgcacgcggg tgcggggaga
    26581 agataatggc gttacgggtc ttcagactga tcagcgattt gaagatagca gttgaagtcg
    26641 ggttagtggt cggaacgata ccgcaaataa taccgattgg ttcagcgata gtgatggtac
    26701 caaaagtgtc gtcttcagac agaataccac aggttttttc atctttatag gcgttgtaga
    26761 tatattcaga agcaaagtgg tttttgatca ctttatcttc gacgataccc atgccggatt
    26821 cggcaacggc cattttcgcg agtgggattc gagcatctgc agcagccaga gcggcggcgc
    26881 ggaagatttt gtctacttgc tcttgagtga aactggcata ttcacgctgg gctttcttta
    26941 cacgctctac gagtgcgtta agttcagcga cattagtaac agccataatg ctctcctgat
    27001 aatgttaaac ttttttagta aatcatctgc tcgaatacga gagtatagtc agtgcggtga
    27061 tgatttgctt aacctatgaa aatcaaaagc ttactcgcgc tcacactcac tgtgatttac
    27121 taaaagagtt taaacattag agttattatc tctaatgcgt cacttccagg tggcgtaagc
    27181 aagattactc acttctgggt actgattacg tgatccaaat caaatttttt gcaaagctga
    27241 cacctttcag catcgctttt cgccattata gctaacagtt aataaattgt agtatgattt
    27301 ggtggctaca ttagcatgtt ttgcacaact agataacaat aacgaatgat agcaatttta
    27361 agtagttagg aggtgaaaaa tgctgtcaaa aggcgtattg tcagcgcgtc ttttcaacct
    27421 tatttatggc taacattatc cggcttttgc ttcggagcta accgtgattc agaccttttt
    27481 tgattttccc gtttacttca aatttttcat cgggttattt gcgctggtca acccggtagg
    27541 gattattccc gtctttatca gcatgaccag ttatcagaca gcggcagcgc gaaacaaaac
    27601 taaccttaca gccaacctgt ctgtggccat tatcttgtgg atctcgcttt ttctcggcga
    27661 cacgattctg caactttttg gtatatcaat tgattcgttc cgtatcgccg ggggcatcct
    27721 ggtggtgacg atagcgatgt cgatgatcag cggcaagctt ggcgaggata aacagaacaa
    27781 gcaagaaaaa tcagaaaccg cggtacgtga aagcattggt gtggtgccat tggcgttgcc
    27841 gttgatggcg gggccagggg cgatcagttc taccatcgtc tggggtacgc gttatcacag
    27901 cattagctat ctgtttggtt tctttgtggc tattgcattg ttcgctttat gttgctgggg
    27961 attgttccgc atggcacctt ggctggtacg ggttttacgc cagaccggca tcaacgtgat
    28021 tacgcgtatt atggggctat tgctgatggc attggggatt gaatttatcg ttactggtat
    28081 taaggggatt ttccccggcc tgcttaatta attcctttca aatgaaacgg agctgccatg
    28141 ctccgtttac ttcgtcatta tttttacttt gttcccg
//
LOCUS       DADLTD010000030        27865 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLTD010000030 DADLTD010000000
VERSION     DADLTD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.030"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..527
                     /locus_tag="JN701_001041"
     CDS             <1..527
                     /locus_tag="JN701_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7363559.1"
                     /translation="KYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEI
                     INKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHN
                     GNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRN
                     GLMPLIRRRTMLQL"
     gene            893..1960
                     /locus_tag="JN701_001042"
     CDS             893..1960
                     /locus_tag="JN701_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7363560.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1957..4692
                     /gene="rbbA"
                     /locus_tag="JN701_001043"
     CDS             1957..4692
                     /gene="rbbA"
                     /locus_tag="JN701_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY7363561.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4692..5816
                     /locus_tag="JN701_001044"
     CDS             4692..5816
                     /locus_tag="JN701_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY7363562.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5889..6164
                     /locus_tag="JN701_001045"
     CDS             5889..6164
                     /locus_tag="JN701_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY7363563.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6161..6520
                     /locus_tag="JN701_001046"
     CDS             6161..6520
                     /locus_tag="JN701_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY7363564.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="JN701_001047"
     CDS             complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="JN701_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY7363565.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="JN701_001048"
     CDS             complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="JN701_001048"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY7363566.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="JN701_001049"
     CDS             complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="JN701_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY7363567.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="JN701_001050"
     CDS             complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="JN701_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY7363568.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="JN701_001051"
     CDS             complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="JN701_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY7363569.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="JN701_001052"
     CDS             complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="JN701_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY7363570.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="JN701_001053"
     CDS             complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="JN701_001053"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY7363571.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12715..13764)
                     /locus_tag="JN701_001054"
     CDS             complement(12715..13764)
                     /locus_tag="JN701_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7363572.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVTITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMIGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYACILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13896..15113
                     /locus_tag="JN701_001055"
     CDS             13896..15113
                     /locus_tag="JN701_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7363573.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15117..15674)
                     /gene="dcrB"
                     /locus_tag="JN701_001056"
     CDS             complement(15117..15674)
                     /gene="dcrB"
                     /locus_tag="JN701_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY7363574.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15747..16412)
                     /locus_tag="JN701_001057"
     CDS             complement(15747..16412)
                     /locus_tag="JN701_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY7363575.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16633..16878
                     /gene="tusA"
                     /locus_tag="JN701_001058"
     CDS             16633..16878
                     /gene="tusA"
                     /locus_tag="JN701_001058"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY7363576.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16980..19178)
                     /gene="zntA"
                     /locus_tag="JN701_001059"
     CDS             complement(16980..19178)
                     /gene="zntA"
                     /locus_tag="JN701_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY7363577.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19252..19878)
                     /locus_tag="JN701_001060"
     CDS             complement(19252..19878)
                     /locus_tag="JN701_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY7363578.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20019..20378
                     /locus_tag="JN701_001061"
     CDS             20019..20378
                     /locus_tag="JN701_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY7363579.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20381..20650)
                     /locus_tag="JN701_001062"
     CDS             complement(20381..20650)
                     /locus_tag="JN701_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY7363580.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20640..21236)
                     /gene="rsmD"
                     /locus_tag="JN701_001063"
     CDS             complement(20640..21236)
                     /gene="rsmD"
                     /locus_tag="JN701_001063"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY7363581.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21386..22879
                     /gene="ftsY"
                     /locus_tag="JN701_001064"
     CDS             21386..22879
                     /gene="ftsY"
                     /locus_tag="JN701_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY7363582.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22882..23550
                     /gene="ftsE"
                     /locus_tag="JN701_001065"
     CDS             22882..23550
                     /gene="ftsE"
                     /locus_tag="JN701_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY7363583.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKVWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23543..24601
                     /gene="ftsX"
                     /locus_tag="JN701_001066"
     CDS             23543..24601
                     /gene="ftsX"
                     /locus_tag="JN701_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY7363584.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24846..25700
                     /gene="rpoH"
                     /locus_tag="JN701_001067"
     CDS             24846..25700
                     /gene="rpoH"
                     /locus_tag="JN701_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY7363585.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25971..27074
                     /locus_tag="JN701_001068"
     CDS             25971..27074
                     /locus_tag="JN701_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7363586.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27273..27656)
                     /gene="panM"
                     /locus_tag="JN701_001069"
     CDS             complement(27273..27656)
                     /gene="panM"
                     /locus_tag="JN701_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY7363587.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6596 a         7233 c         7744 g         6292 t
ORIGIN      
        1 ccaaatacaa aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt
       61 acattatagg atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      121 aattagcgga aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
      181 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      241 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
      301 cacataacgg taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
      361 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      421 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      481 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      541 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      601 ttcctgcaac tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc
      661 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      721 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
      781 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
      841 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa
      901 gagtaagcgc catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc
      961 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1021 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     1081 gaaagaaggc cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgtgtgtt
     1141 gcaggaacag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc
     1201 cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa
     1261 tcaacgccag gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc
     1321 ccaacgaggg gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc
     1381 ccgagctgcg ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc
     1441 ggcacgtacc aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg
     1501 gcgcattgcc gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1561 gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1621 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa
     1681 actgggcggt gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     1741 catcagtttt gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     1801 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     1861 tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact
     1921 tccgtggcct gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     1981 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2041 aacaatatca ctctcgatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc
     2101 gtcgggaagt cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat
     2161 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2221 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     2281 aacgtcgatt ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     2341 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     2401 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     2461 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2521 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2581 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2641 accggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt
     2701 attaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     2761 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     2821 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt
     2881 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc
     2941 agcgaaggtg aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc
     3001 cgtcgtgtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3061 aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3121 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3181 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3241 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     3301 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg
     3361 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     3421 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3481 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     3541 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3601 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     3661 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3721 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     3781 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     3841 cgtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     3901 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     3961 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     4021 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac
     4081 ccggacgtaa aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg
     4141 atcccgtcaa tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4201 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4261 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4321 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     4381 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     4441 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4501 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4561 actatcgccc gcgggacgtt ctcgaaagcg ctggatctga ctgatatgtg gcaacttttt
     4621 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4681 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     4741 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     4801 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     4861 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     4921 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     4981 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5041 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5101 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5161 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5221 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5281 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     5341 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     5401 gattatcgct ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     5461 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5521 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5581 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5641 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5701 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     5761 cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     5821 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     5881 gacatgccat gagaaccaag gtacaggctt tacggaagaa acaaaaaaat acattggatc
     5941 aaatatttaa aactcctgtt cctcaaggga tcaagtggtc tgatatagag tcactggtta
     6001 aagcattagg cggagaaatt aaggaaggaa gaggttcgcg ttgtaagttc atactaaata
     6061 tgagcgttgc gtgtttccat cggcctcatc cgtcgccaga taccgataaa ggcgctgtag
     6121 aaagcgtgcg tgactggtta ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc
     6181 aaactctatg gagattgccg ggcaaccagc tgttattacc tacgtgccag aactgaatgc
     6241 atttcgtggc aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca
     6301 ggggctgcaa aaagaagggg aactttccct gctagagtat cttgaggatt gtaaagctgc
     6361 gggtattgag ccgtatgcgc gtacagaaaa gattaagacc tttacgttac gctatccaga
     6421 atcgctgagt gaacgcctta ataatgcggc tgcgcagcaa caagtctctg ttaataccta
     6481 tattattgaa acactgaatg agcgtttgaa tcatttataa attttagcgg cgtacattac
     6541 gcagaatatc tgcctttaca ggatctatct atgcaactga acttaatttt ataaattaag
     6601 ttcagttaat atcttttctt tcctcgtttc agaaataact caatcttcct tcggcaagca
     6661 ctgcaaatgc ccatgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
     6721 gtcgcccata tcacctttca gcacggcgat ttccagacag tcgtcgtgat tgatatgcac
     6781 atgcagcgtg gcgacggaga gatcgtggtg atggtgctgg gtggagacaa tgcggctggc
     6841 taagtcgcgt ttttcgtgtt catacacata ggagagcacc gcgaaacctt gcgtgccgtg
     6901 ctgctgagtg gcctcctggg ccagggcgct acgcagaata tcgcggatag cttcggaacg
     6961 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     7021 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg gcgcacgggg
     7081 aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta
     7141 tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac ccgctggcaa
     7201 aagcgttcta caagacgtaa gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt
     7261 tgcagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag gttagaaacg
     7321 gtttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag gcagacgcgc
     7381 tggagctggc cgccgcttaa ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg
     7441 accgccttca gcatttcgct ggcgcgcgcc agttgttcgg attttttcag tgatagcagg
     7501 tggcgcatcg gttcacgcag gatctcgcgc acggttttgc gcggattcac ggcactgatg
     7561 gagtcctgaa acaccatctg aatatcacgg cggaaggctt tacgttgggc gcgattaagt
     7621 ttcgccagcg attcgccgcg ccagctaata ttcccttgtg agggagactc taagcctacc
     7681 agcagccgcg cgagggtgct ttttccgcag ccgctgcgtc ccagcagggc gacggtttcg
     7741 ccgcttttca gggtcaggga aacgttattc agcaccgcct gatgttgatg ttttccgcta
     7801 aatccaccgt gggcatagtg atgggaaagg ccgctgacgt taagtaaggt catgatgcca
     7861 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg
     7921 gggcgttaaa cagcgtttct acatcgccct gttcgacaat tttaccttgt gacatcaccg
     7981 ccacgtcatc cgccagacgc gccacaacgc ccatatcatg ggtgaccagc agcattcccg
     8041 gcgcttgttt ttgcataatg ctttccagca gatcgaggat gcgcgcctgt gctaccacgt
     8101 cgaggtcggt ggtcggttca tcggcgatga taaacggtga ttcacacagc accgccatcg
     8161 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc
     8221 gcgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
     8281 gtttccctaa cgccaggcag gtttcccgcg cgtgggtgtg catggtgtgt agcggattaa
     8341 aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg gcgcagggat
     8401 aaaccgcttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcca gcgggcaaaa
     8461 tgcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg actaacgcca
     8521 gcacgcgccc gcgttgcagg gttaacgata caccgtgtac cagcggctgc gcggcctgta
     8581 gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg tcaccagatg
     8641 agggtccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
     8701 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact ggcgcgcgtc
     8761 gttaatcatc acgccccatt cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat
     8821 ccccgagacg tgcagcatca tatggccgat atccagcgtt gccagcacca gcagcgaagg
     8881 gatcaccgcg cctgccagat gatcgacaaa cactcgcaca tggcccgcac cggaaagccg
     8941 tgacgccagc acaaactcgc gttggcgtag tgaaatcacc aggctgcgca ccatgcgtgc
     9001 ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc cggtgccgag
     9061 cacgccaacc ataaagaacg acagaatcga ggtcgggaag gtcataaaca tatcggcgac
     9121 gcgcatggtg gcctgatcaa cgcgcccgcc aatcaacccg gcgctgccgc caataaccag
     9181 ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca cgcgggtcgc
     9241 tgccatcagc cgcgagaaaa tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc
     9301 atccggcgaa agcaggtgcg acggcaaatc aatcgcctgg gggtcatacg gcagccacca
     9361 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgtaccga
     9421 ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatgcgcggg
     9481 tccagcgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccaccatc
     9541 atcagcgtaa agcactgaat caccggatag tcacggttaa aaatcgccga caccgcatag
     9601 cgcccgacgc caggccaggc aaagatgttt tcgataatca tcgtcccgcc aatcaattcg
     9661 ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg
     9721 cgttcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg acgctgaccg
     9781 gcgacgtcca gcatactggc gcgcagcagt cgcgcgttaa tcgccagcga cataaaggcg
     9841 atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc gggtagccat
     9901 tgcagataca ccgaaaacgc catcactagc aaaaatgcca gccagaagtt gggcatcgac
     9961 acgccaagaa acgcgataaa gcgcacggcg aaatccggca aacgatcgcg gtggcgcgcc
    10021 gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc acctgcaagt
    10081 tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgttg gctggcgaat
    10141 gagataccaa agtcaagatg cagcgccttc cacaaccagg tgccgtactg gacgtacagc
    10201 ggctgatcca gtccgagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
    10261 ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag catcagaaaa
    10321 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacgtaa
    10381 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg
    10441 cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg taactgatag
    10501 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagc gcctgacgtt
    10561 gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggtttgtcgg
    10621 ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
    10681 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
    10741 gctgacgagc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
    10801 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
    10861 acgacagttc aatgcgcagc ggctgaccat ttttctcgcg gatgtctttg cccgcaggca
    10921 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
    10981 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
    11041 tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta agagcttcac
    11101 gtactgccag ctcattggtg ggggttttgg cggtattaag cgccagcatc acggtttcga
    11161 tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg aaggtatcga
    11221 gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca aacgccaccg
    11281 cgcgggtagt cgggtccggg atgacgttaa aggtgatctt tttaatcgct ggcttttcgc
    11341 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgcaaaa
    11401 tccacggtcc ggtgccaatc ggcgctttaa ttccgttcat ggtttcatgg tttttaaact
    11461 gcgagggagc gataaaacgg aaaggacggg gcagggccag ttcttgcagg aaaggatagt
    11521 aggcgctttt cagggtaatt tgcagctctg ttttactgag tgctttaaca tcaacaatct
    11581 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg cggaagtttt
    11641 ctgccgccgc ctcggcatcg aacggttcac cgttggataa tttcacgtca tcacgcaggg
    11701 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    11761 ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg gcgaacatct
    11821 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtagtga
    11881 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    11941 tgcggcggag tgtggagagc atgataaccc caatggatta aaatagatgg cggaaataag
    12001 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    12061 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    12121 gggggtgggg gtgcagatcg caaggcttaa attttctagc tgacaatggc tgactgacag
    12181 cgaggagtga taggtgctat ctacgctgac gatttgccag gcgctgccgc cacgctgttt
    12241 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    12301 cacggcgtcc atttcagcgt gttccccgag gctgaacacg gcgttcgcca gccagcgcca
    12361 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cgctcaacag
    12421 cagagcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa
    12481 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    12541 caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa
    12601 acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    12661 atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc
    12721 cggtaaccga cttttcggtc tgcctggccc cagtaaaatc gccagtttgc taccgccttt
    12781 ggtggtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    12841 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    12901 caggcgatgg cccatcatcc gtggttctaa aatattgccg atgaccatat ggaccactaa
    12961 aaacaatgcg ccgaccagaa tacatgcgta aacaccatta aacagcagca cctgaatcat
    13021 tggcggtacg gcggaaatta ccgcgccgat attgggcacg tagttgagca aaaacgccag
    13081 tactgcccac atcagcgcaa actgtacgcc cattagcgcc agccccagcc agacgatgac
    13141 acctgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc ctttaagtgc
    13201 gcggtgtaat cctgcgatgt gaatctgtgg gttattcagc gcaaaacgca ttttgtaagg
    13261 gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa gcacgctcgc
    13321 cattgccccg gaaagcccga tcattagcgc tgtggtgaag gtaaccactt tttccgagtc
    13381 catccgctgc agcattcgct ccggcgacat atgcaaatta agaaaaggca acatctcctg
    13441 caatttaaaa agtttgcgcg tcagctcctt attaaacttc ggcagcatag agataaattc
    13501 gttaaacgat gccgccagta cgccgactag cgcggttagt gcgatcagca tcaccaccac
    13561 tacaatcgta atggtaacgg ggcgttgtac tccccgacga ataaaccagg taacgagcgg
    13621 gttgaggacg atggcaaaaa acagcgccag taacagctgc acaatgatat ctgccgctgc
    13681 gtgaatgccc gcgaggatca ctaccagcga ggccaacttg agcagaatgt gcatgcccgt
    13741 tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtgt taagtgtagc
    13801 gggagtcacg tcagcaatat tctgattctt actgctgatt ttcacgtcag cacgtggtaa
    13861 aaatgaaaca ctgttgtaaa aatgtggtga tcctcatgcc cgaacccgta gccgaacccg
    13921 cgctaaacgg attgcgcctg aatttgcgca ttgtctccat tgtcatgttt aacttcgcca
    13981 gctacctcac catcgggttg ccgctcgctg tattaccggg ctatgtccat gatgtgatgg
    14041 gctttagcgc tttctgggca ggattggtta tcagcctgca atatttcgcc accttgctga
    14101 gccgtcctca tgccggacgt tacgccgatt tgctgggccc caaaaagatt gtcgtcttcg
    14161 gtttatgcgg ctgctttttg agcggtctgg gatatctgac ggcaggatta accgccagtc
    14221 tgcccgtcat cagcctgtta ttactttgcc tgggacgcgt gatccttggg attgggcaaa
    14281 gttttgccgg aacgggatcg accctgtggg gcgttggcgt ggttggctcg ctgcatatcg
    14341 ggcgagtgat ttcgtggaac ggcattgtca cttacggggc gatggcgatg ggtgcgccgt
    14401 taggcgtcgt gttttatcac tggggcggct tgcaggcgtt agcgttaatc attatgggcg
    14461 tggcgctggt ggccattttg ttggcgatcc cgcgtccgac ggtaaaagcc agtaaaggca
    14521 aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct gtacggtatg gcactggcac
    14581 tggcttctgc cggatttggc gtcatcgcca cctttatcac gctgttttat gacgctaaaa
    14641 gttgggacgg tgcggctttc gcgctgacgc tgtttagctg tgcgtttgtc ggtacgcgtt
    14701 tgttattccc taacggcatt aaccgtatcg gcggcttaaa cgtagcgatg atttgcttta
    14761 gcgttgagat aatcggcctg ctactggttg gcgtggcgac tatgccgtgg atggcgaaaa
    14821 tcggcgtctt actggcgggg gcagggtttt cgctggtgtt cccggcatta ggtgtagtgg
    14881 cggtaaaagc ggttccgcag caaaatcagg gggcggcgct ggcaacttac accgtattta
    14941 tggatttatc gcttggcgtg accggaccac tggctgggct ggtgatgagc tgggcgggcg
    15001 taccggtgat ttatctggcg gcggcgggac tggtcgcaat cgcgttatta ctgacgtggc
    15061 gattaaaaaa acggcctccg gaacacgtcc ctgaggccgc ctcatcatct taaaacttac
    15121 tgaataacca gcgtattaat gatgttttct gcggtggtct gcgctttttg ctgatcgtca
    15181 gcgggcagcg taatttgcat ggtcagcagt tgattaccca cgttacccag aataacggaa
    15241 gagtacgccg tctggccttt cgcggagata atactgtcta actgctgcat tttgtgacct
    15301 ttcagctcaa tggctttatt ggttaccact tgcagctgcg gatcgcggct acgttgctga
    15361 tcttccagac gcttcgccag caccgccaga tcttctttcg gatcatcgcc catgatgacg
    15421 atgactgctt tctgcccggt ggcgtcggac cagacatgca tgttattggc ctgcgttccc
    15481 agcttaccgc tctggtcggt catatccgct ggcagcgaga aacttaactt gccatcaagc
    15541 aggttgacgg gattcccggt agcgttattt tccgcgaccg aaccctgcgc cgtagcgtta
    15601 gtgtctttat catcacaggc cgcaagcccc ataaccagca ggccaattcc gacatattta
    15661 accagattgc gcattgactt cttcctttcg ataaacggcc ataacggctc attcatccat
    15721 cttatcacaa ctctgataac gaacctttaa ctcgcctgca aagcgttgat ttcggattta
    15781 tctgccagtc ttttcaacag catattgagt aatacgccat acattggcag gaagaaaacg
    15841 atactgatta acactttgaa acagtaatcg accagcgcga tttccatcca gtgttcagcc
    15901 ataaaggcat ccgggctacg ccagaaggca atgaagaaaa aggccagcgt gtcgctgacg
    15961 ttaccgaaca gtgtggacgc tgtcggtgcc agccaccagc ggcgactctg acgcaggcgg
    16021 ttaaaaacgt gcacgtcgag gatctgtccc agcgcgtagg ccatgaaact ggcggtggcg
    16081 atacgggcga caaacaggtt gaagtgggcg agtgcgccga atccctgcca ggaacccata
    16141 tagaatagcg acgagatgac gtaggagatc agcaacgccg ggatcatcac cgcaaagatg
    16201 atacgacggg ccagcggtgc gccaaaaata cgcacggtca ggtcggtagc aaggaaaata
    16261 aacgggaagc taaacgcgcc ccaggtggta tggaaaccca aaatggagac gggaagctga
    16321 accagatagt tactggaggt gatcaccagc agatgaaata acgataacca gaacaacgcc
    16381 ttatagcgtt gagtttgcga gaaaacgttc atattgtacc tttttgatta accattgggg
    16441 tgagggaacc caatacgtac gacacgtctg ttaccttaac ataataacgt gcttaccctt
    16501 ttttcgagcc gccgcatgat actgctttac gttgacaatg caatggttaa ttttcacgca
    16561 atcgttaacc tggtttgctt acggactcac agggcgtaaa ctagcgccgt ttttttatgt
    16621 gatgagaaga aaatgaccga tctcttttcc agccctgacc acacactcga cgcacttggc
    16681 ctgcgctgcc cggaacccgt aatgatggtg cgcaaaactg tgcgcaatat gcagcccggc
    16741 gaaacgttgc tgattatcgc cgacgatccg gccactaccc gcgatattcc tgggttttgt
    16801 acctttatgg aacacgaact ggttgcgaaa gagacggatg gactgcctta tcgttatttg
    16861 attcgtaaag gcggttgatg ggggctgatt ggcttcgatg ccgccttttc ccctcaccct
    16921 aaccctctcc ccagaggggc gaggggaccg atcgcgctca atgttgcgat cggtttgcct
    16981 tatctcctgc gcaacaatct taacgcattc gctgtcacca gcaccgtcgc acccgtatct
    17041 gccagcaccg ccagccatag tccggtcatc cctaaaagcg tggtgacgag gaagatccct
    17101 ttcagtccca gcgcaatggt gatgttctgg cggatattgg cgtgagtggc gcgtgccagt
    17161 tcaatcattt gcaccaggcc gcgcaggtgg ttatgggtta atgctgcatc agcggtttcc
    17221 agcgccacgt ctgtgccact gcccattgca atcccaatgg cggcagcttt catcgctggc
    17281 gcgtcgttaa taccgtcacc gaccatcgcc agcggcgcat gttgattcag ctcggtcacc
    17341 gctttgactt tatattccgg caacaggccc gctttaaact ccagccccag ctccccggca
    17401 attgccgccg ctgcgcgtgg attatcgccg gtgaggatca cccctttgac gcccagcgcg
    17461 ttcagttcac tgatggcagt tgcagcatcg gcgcgcaggg tatcctgcaa tgcaatgata
    17521 cccagcacgt catcattacg tactaccagc acaaccgttt gcccggcgct ttccagttca
    17581 ttaatcaaac cagcaaatgc atcagcggga tgtttcccgg cagcgcatat caacacgcgc
    17641 tcaccgttaa cctgcgcttc aatgccagac ccgaccagcg cccgctgtga ttcggcggcg
    17701 ggaatggcga gtgcagcaac ctgtgcttcg cgcacgatgg cctgcgccag tggatgcgtc
    17761 gcgctttgct cgaccgccgc cgccagtgtc agcagttcag attcactaat acccgttgcc
    17821 ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg taccggtttt atcaaacgcc
    17881 acctgagtaa cacgacctag ttgttccagc gccgctccgc ctttaatcaa cgccccacga
    17941 cgcgctgctg ccgccagccc ggaggtaatc gccgcgggcg tggagataac taacgcacac
    18001 gggcagccaa tcagcagcag cgtcaggcct ttataaatcc actcctgcca gctggcggca
    18061 aacagcagcg gtggcaccag cgtcaccagc agggcgacgg ccataatcgc tggcgtataa
    18121 atacggctga aacggtcgat aaaccgctca atgggggcgc ggcgctcttc ggcttcttcg
    18181 atcagtttca gaatccggtc aatggcgctg gcacccggtt ctgacagcac ttccagcgtt
    18241 accagacggt ctacgctggt ggctcctgca ggaactttat cgcccgtcgc gcgctccacc
    18301 ggaatggatt ctccggtcag ggcgctttca tcaaaactgg caaacggtga gagcagttta
    18361 ccgtcggcag gcaaacgccc acctgcggcg acttcaatca catcgccagg ccgcaggctg
    18421 ttaatcgcca cctcttcccg ctcaccgtta cgcaggcgcg tggcggtttc tggtttcagc
    18481 gccattaacg cgctaacgcc ctgacgcgcg cggctggcgg cccagccttc cagtcgttca
    18541 ccaatcaaaa acagcagcaa caccatcgca gcttcagccg ttgcgccaat aaacagtgca
    18601 ccaatagcgg ctacgctcat taaggtttca atggcgaagt agctgccgga tttgatcagc
    18661 cgtaatgcct gatgagcaat cgggtacagc ccaaccagcg tggtcgcgat aaacgccagt
    18721 tgcccgaacg gatgattgaa ctgctccaga ccccagctga ttgccatcat cacgattagt
    18781 gtaatcagcg gcagattctc tttcaggcgc gatgcttgtg gctcgtcggc ggcctgctca
    18841 tcgcgcaggg aatagcccgc tttttgcacc gcagattcaa cttgtgcacg aatgtcattg
    18901 tcggcatcaa ccaccagttt ttcggtggcg aacaacacct gcacctgatt cacacctgca
    18961 agctggcgca cggcattttc taccttgcgc gcacaggcgg cgcagtccat gccactgact
    19021 ttccagctat agcgggtgcc ggagacgttt tcagagagcg ttggcgagct ggagcatgcg
    19081 ccgtcgcagc aacagtcgtt ggcgttctgt accgtggtta gcggtttgaa cgcagcaaat
    19141 tgaggggctt tcttgccgtg attgtcaggt gtcgacatgg catactccgg ttaagttttt
    19201 tctcattaac cgaaggatac actctggagt cgactccaga gtcaagtttt atcagaaata
    19261 cagtgagcgg acgatgagga agtgaccggc aaagtagcag gccgcagcaa tcgcgttatc
    19321 cgcacggaag cgtcggcgat agtggctccc cagccagaca aagttactga taaacagcaa
    19381 cgaagcgcca acaaacgcag agagcgccgg agcggtcgga cggaagaacc acagttcacc
    19441 tgccagccac accatcacca gcgtcatgcc gataaacgtg cagataggcc aacgatactc
    19501 ttccagccgc gtccagataa tcgccagtaa cagcgcaccc agaaccagca acaccagtgg
    19561 cagaggccag aagaaagaga gcgtcatctg actggcgaaa tagatggtgt acagcaggtg
    19621 cgagaggaaa aacgcgccga tggcgtacat cagacgttga cgtggcaaca gggttagcgc
    19681 atcgcccagc agtgaggcgc acagccctgc cagcaccaga tagctaatgg cgtcgaacat
    19741 cggcgcttgc caggccagta acagcaggag aagaagggtt aacggtttaa acacccagcg
    19801 ttgccaggtt ggcccacgat acgatgcatc cacagatagc catgcggaaa gacagacagc
    19861 gataaacgac caaagcatct tagttccttg aattcgttat tttttcgtaa gcggaatgcc
    19921 tacggtcttg cttccagttt agtggcgagg gtggacggat gacaacacca taactagcag
    19981 ggtatgctta tttccgcatt ttccgatgag ggataaagat gagcaaacca cctcttttct
    20041 ttattgttat cattggctta attgtcgtcg ccgcatcgtt tcgttttatg caacagcgac
    20101 gggaaaaagc tgataatgat atggctccgc tccagcaaaa gctggtggtg gtgagcaaca
    20161 agcgggaaaa accgattaac gatcgccgtt cgcgccagca ggaagtgact ccggcaggca
    20221 ccagtatgcg ctatgaggca agcttcaaac cgcaaagcgg aggaatggag cagacgtttc
    20281 gcctcgacgc ccagcagtac catgccctga cagtgggcga taaaggtacg ctgagctata
    20341 aaggaacgcg ctttgttagc tttgttggcg aacaataagg ttatttttta actttaaatt
    20401 tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa aatccgcacc ttttcggcgg
    20461 tggtcatctg cgggccatct ttcggtaaag tggatttcaa tagcgccagc tgcataccat
    20521 gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat attcagcggg cgcgggaagg
    20581 gatgcaccag attgaggatt aaaaatcccc aaacgcagag cattaacaaa cgaccaatat
    20641 taatcagcat cactttctcc ttgtgcttcg cgttgataca gccgataagc cacctgaccc
    20701 gccacttttt cccgatgtaa tgaccagttt gccggaacag tgggcagacc gttttcgact
    20761 tcgctttcga cataaatcag ggcttcgtca gccagccagc cgttatcttc cagtaaattt
    20821 atcgtctctt ctaacaagcc acggcggaac ggtggatcga caaacacgat attatgcggt
    20881 gtaccttttt gcgccaggaa tgacatcgcg ttgctgttca ccacgcgtgc attgcctgct
    20941 tttagtgtcg ccagattctt aattaactgc tgagaaaccg cgcgatccat ctcaatcaac
    21001 gtggcccccg cagcgtagcg cgataacgct tccagtccca gcgcgccgct cccggcgaag
    21061 caatccagac attgggcgtc aacaatgacc ggagccagcc agttaaacaa tgtttcgcgt
    21121 acgcggtcgg tggtggggcg cagacctggg ctatcaggaa ccgggagttt acggcctcgc
    21181 cactgcccgc caataatgcg gatttggccg ctgccggaat gattcggttt tttcatgata
    21241 aattgctcaa tccgccagat aacacatact tgcaggcggt gatgtgaatt aagttaagtg
    21301 atagactatt tcatcatttt tttagctgct atgtacatag cgttaacgct gtgccatgaa
    21361 gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa aaacgtggct ttttttcctg
    21421 gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag acagaagttc agaatgaaca
    21481 accggttgta gaagaaatcg ttcaggcgca agagcctgcg aaggcttctg aacaagccgt
    21541 cgaagagcaa ccgcaggcgc atactgaagc cgaggcggaa acttttgctg ccgacgttgt
    21601 ggaagtcact gaacaggttg ctgaaagtga aaaagcgcag cctgaagcgg aagtcgttgc
    21661 acagccggaa ccggtcgtag aagaaacgcc ggagccagtg gctatcgaac gtgaagagct
    21721 gccgttgccg gaagacgtca acgccgaaga ggtttcgcca gaagagtggc aggctgaagc
    21781 ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag aaatcaccga
    21841 cgaagagctg gaagcacagg cgctggctgc cgaagcggca gaagaggcgg tgatggtggt
    21901 tcctccggta gaagagcagc cggtggaaga aatcgctcag gagcaggaaa aaccgaccaa
    21961 agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc
    22021 cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga
    22081 agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct
    22141 gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa
    22201 agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac
    22261 gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaactacga cgattggtaa
    22321 gctggcacgt cagtttgagc tgcagggtaa atcggtgatg ctggcggcgg gcgatacttt
    22381 ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat
    22441 tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc aggcagctaa
    22501 agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca
    22561 cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc
    22621 gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa gccaggccaa
    22681 actgttccac gaagccgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc
    22741 gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg
    22801 tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact
    22861 ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt
    22921 gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg
    22981 accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg
    23041 cccagcgccg ggaaagtctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa
    23101 gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac
    23161 cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat
    23221 attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac
    23281 ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg
    23341 aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg
    23401 gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca
    23461 acgcacgaca tcaacctgat ttcacggcgt tcctatcgca tgctcaccct gagcgatggt
    23521 cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt
    23581 tggtgggcgt ctggatcgct tccgtaaatc ggtcggcggc tcaggcgacg gcggtcgtaa
    23641 cgcaccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca acgttttcaa
    23701 cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc
    23761 cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat
    23821 ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt
    23881 ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc
    23941 cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg
    24001 taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt
    24061 ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga
    24121 tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg
    24181 tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt
    24241 ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga
    24301 ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct
    24361 gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat
    24421 tctggtgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaattt
    24481 tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat
    24541 tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata
    24601 ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt
    24661 tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt
    24721 gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa cctcgttgct
    24781 cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga
    24841 tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct
    24901 acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg
    24961 aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc
    25021 ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga
    25081 ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg
    25141 tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc
    25201 gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc
    25261 tgcgtaaaac taagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc
    25321 gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg
    25381 acatgacctt tgatctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc
    25441 tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag
    25501 agcaggcggc aaaccgtctg accgacgcga tgcaaggtct ggacgaacgc agccaggaca
    25561 tcatccgcgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc
    25621 gttacggtgt ttccgctgaa cgtgtacgcc agctggaaaa gaacgcgatg aaaaaactgc
    25681 gcgctgctat agaagcgtaa tttccgctat taagcagaga accctggatg agagtccggg
    25741 gtttttgttt tttgggcctc tacaataatc aattccccct ccggcaaaac gtcaatcccc
    25801 acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa tggggtatgt
    25861 tttagcagag tatgctgcta aagcacgggt agtcatgcat aaaacgaaat aaagtgctaa
    25921 atcacaacac cacaacacac gtaataacca gaagaatggg gattctcagg atgaatataa
    25981 agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag
    26041 aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc
    26101 aggagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag
    26161 gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg
    26221 tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat
    26281 caacgcagcc tgcgtctgat atctacgaag acgaaggcat tttaatgatc accccagcgg
    26341 caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg
    26401 actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta
    26461 ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg
    26521 gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag
    26581 atttctcaac gctggtggcg cgtctgaaaa aagagaatat cgacttcgtt tactacggcg
    26641 gttatcaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc
    26701 agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag
    26761 cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggcg aacaaaccca
    26821 ttgttgacgc catcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct
    26881 acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgagatcg
    26941 ccaaatacct gaaagcgaac tccgtggata ccgtgatggg tccgctgacg tgggatgaga
    27001 aaggtgatct gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga
    27061 ccgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag
    27121 tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag atccaaagaa
    27181 atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc
    27241 gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc
    27301 gccgtaaatc ccagtgcctg cataaacgcc gtcatcacac cgcgatcttc cacgcctgca
    27361 tccgccatcc accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat
    27421 tgccccacac cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg
    27481 gtgccgctta aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag
    27541 atacggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc
    27601 aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt
    27661 aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc
    27721 tcttttttat ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa
    27781 acattagaga atgaaaaata cccagcataa tcccctgaat ggtagtgaat tattccgccc
    27841 tttgtgccgt tattttatgc tgaca
//
LOCUS       DADLTD010000031        27539 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLTD010000031 DADLTD010000000
VERSION     DADLTD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.031"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            109..261
                     /locus_tag="JN701_001070"
                     /pseudo
     CDS             109..261
                     /locus_tag="JN701_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(317..1933)
                     /locus_tag="JN701_001071"
     CDS             complement(317..1933)
                     /locus_tag="JN701_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY7363588.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(1934..3460)
                     /locus_tag="JN701_001072"
     CDS             complement(1934..3460)
                     /locus_tag="JN701_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY7363589.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(3463..5130)
                     /locus_tag="JN701_001073"
     CDS             complement(3463..5130)
                     /locus_tag="JN701_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7363590.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHCILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(5127..7235)
                     /locus_tag="JN701_001074"
     CDS             complement(5127..7235)
                     /locus_tag="JN701_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAY7363591.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(7222..8043)
                     /locus_tag="JN701_001075"
     CDS             complement(7222..8043)
                     /locus_tag="JN701_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HAY7363592.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(8203..10032)
                     /gene="glmS"
                     /locus_tag="JN701_001076"
     CDS             complement(8203..10032)
                     /gene="glmS"
                     /locus_tag="JN701_001076"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY7363593.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(10194..11564)
                     /gene="glmU"
                     /locus_tag="JN701_001077"
     CDS             complement(10194..11564)
                     /gene="glmU"
                     /locus_tag="JN701_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY7363594.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGCDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(11915..12334)
                     /gene="atpC"
                     /locus_tag="JN701_001078"
     CDS             complement(11915..12334)
                     /gene="atpC"
                     /locus_tag="JN701_001078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY7363595.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(12355..13737)
                     /gene="atpD"
                     /locus_tag="JN701_001079"
     CDS             complement(12355..13737)
                     /gene="atpD"
                     /locus_tag="JN701_001079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY7363596.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(13764..14627)
                     /gene="atpG"
                     /locus_tag="JN701_001080"
     CDS             complement(13764..14627)
                     /gene="atpG"
                     /locus_tag="JN701_001080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY7363597.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(14678..16219)
                     /gene="atpA"
                     /locus_tag="JN701_001081"
     CDS             complement(14678..16219)
                     /gene="atpA"
                     /locus_tag="JN701_001081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY7363598.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(16232..16765)
                     /gene="atpH"
                     /locus_tag="JN701_001082"
     CDS             complement(16232..16765)
                     /gene="atpH"
                     /locus_tag="JN701_001082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY7363599.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(16780..17250)
                     /gene="atpF"
                     /locus_tag="JN701_001083"
     CDS             complement(16780..17250)
                     /gene="atpF"
                     /locus_tag="JN701_001083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY7363600.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(17312..17551)
                     /gene="atpE"
                     /locus_tag="JN701_001084"
     CDS             complement(17312..17551)
                     /gene="atpE"
                     /locus_tag="JN701_001084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY7363601.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(17598..18413)
                     /gene="atpB"
                     /locus_tag="JN701_001085"
     CDS             complement(17598..18413)
                     /gene="atpB"
                     /locus_tag="JN701_001085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY7363602.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(18422..18802)
                     /gene="atpI"
                     /locus_tag="JN701_001086"
     CDS             complement(18422..18802)
                     /gene="atpI"
                     /locus_tag="JN701_001086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY7363603.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(19419..20042)
                     /gene="rsmG"
                     /locus_tag="JN701_001087"
     CDS             complement(19419..20042)
                     /gene="rsmG"
                     /locus_tag="JN701_001087"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY7363604.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(20106..21995)
                     /gene="mnmG"
                     /locus_tag="JN701_001088"
     CDS             complement(20106..21995)
                     /gene="mnmG"
                     /locus_tag="JN701_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY7363605.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(22374..22817)
                     /gene="mioC"
                     /locus_tag="JN701_001089"
     CDS             complement(22374..22817)
                     /gene="mioC"
                     /locus_tag="JN701_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY7363606.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(22907..23365)
                     /gene="asnC"
                     /locus_tag="JN701_001090"
     CDS             complement(22907..23365)
                     /gene="asnC"
                     /locus_tag="JN701_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY7363607.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            23517..24509
                     /gene="asnA"
                     /locus_tag="JN701_001091"
     CDS             23517..24509
                     /gene="asnA"
                     /locus_tag="JN701_001091"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY7363608.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(24514..25965)
                     /gene="viaA"
                     /locus_tag="JN701_001092"
     CDS             complement(24514..25965)
                     /gene="viaA"
                     /locus_tag="JN701_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY7363609.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(25959..27455)
                     /gene="ravA"
                     /locus_tag="JN701_001093"
     CDS             complement(25959..27455)
                     /gene="ravA"
                     /locus_tag="JN701_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY7363610.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         6891 a         7127 c         6386 g         7135 t
ORIGIN      
        1 aacatgtcgc cgcgctcaag aactcattgg attactacta tggatcgtct gtaatccccc
       61 ctcaaaacca cggaggctat ttgtagaaaa ttccattaat atgaactcaa ggtgttccct
      121 acggacgaat cagcgatgaa ggtggtgtac ctggctatcc aggcggcgac caagaaatgg
      181 acgatgccga tccgcaactg gaagccggcc atgaaccgtt ttatgatcga atttggtgac
      241 cgtttaaatg gccaccttta actgaccggc aaaagcactt acacaaaagg gtttacaggc
      301 tctttcagtc attcctttaa tgcgtaaatt gctctctaac cactctctca gcccaccctt
      361 ccatatcctc tctcgatact tgcagctccc ccaacccctt tggatgattc acccctctat
      421 gcccgtcttg tccatataat tgatcaaaca aactatttgg ccaattcaat gagctcttca
      481 ccactccacg ccgtatcttt tcaaaatcat tccgccatgt ttcgctatca acgccactca
      541 acactttggt agaaagcatt ttcaccccat ccgatgcatc cacctctagc aatacaaatt
      601 cactgccatt acgttgcacc ccaacggctt taatcactct tggtgagcca tctttacaca
      661 gatgtaatcg gctacgccca acttttggca aaaccaaggt ttcttcaaaa agcacccgac
      721 atgcaaattt agtttttaga attgaaagta gctcatcaaa agcagcaaat cgattagcaa
      781 aaatagagtt gtaattggtt gcatcctgct tcccacccac atctgccgct gctaggacac
      841 cacctaagtg tggctcatca gtactaacca aattactatt ttcacttgtt gttgcttctt
      901 ctttttctaa aatagtttgg ctactttttc ttgccctgcg agatttttca attcgactag
      961 gtctactaaa acttatccat gtcggctcag cctctatgac tcgtgtgtct gtgtctatat
     1021 tggctgtttc ctcatcgtcc aactgtagat gctcatcatc aacaactgac tctgttgaaa
     1081 ccgctatgtg ctgcgtacta ccatcacctg cctccttttc ctgaaaagag gcatggctaa
     1141 tagctgttgt gctaggaagc atagcgttga tttctaaacc tactatttcc tcaactaaat
     1201 aatccttatc ctcgttagaa gaacgtcctt ttacatgtaa tttccaaccc tgcatgggcg
     1261 gagggtcaaa gctaaagcac cagctttcaa cgccgttctt aatttctcta ttttgttgat
     1321 agtgcctaaa aatactttca tacgaatcca taacatcttg atccgaaaac aaccaaacca
     1381 aaagctgcac tacggccgac tgctctaacg cccctttagg aaacgatgaa ctgggtaaga
     1441 tccttatctc taaatgatct cgctcaacct catactgaac gtcgaactct tgctgtaaag
     1501 cggtactgct caaacagctt cgacagaaat aggagttaat taaaaataat gaccgagcta
     1561 gctctaactg tgggatgtga taacgagtct tgctatatgg tgtttcagct tcgtaaataa
     1621 atgaactctg cgctccatcc ttgttgcgta cggaagcaaa ggacgagaga ttaggaaaat
     1681 cgattatttt ggctcgttga agatccgatg cttcaaactc aatcacacga tccgccttat
     1741 ttatttgctt tgttgtactg ttaaccacct tagctcgact taataaaggc aaatgagtaa
     1801 atcgagtcca tttccgttct tgcattggat taaaccaaac aaaaatacgc cactccttat
     1861 gacccgagtt acggaataaa tgaccaatat gtacaacctg cacattgtca ttaaatgtag
     1921 ctagcctaac cactcactct tccccataaa ccatttccaa gaatctttgt gcttcctcag
     1981 ttatccgctc tttagataac gttgcacttc ttaataatcg ccagcgccta agctcaacat
     2041 cttcctgttt aagcttaata aaagcttggc taatccgtct aatttgataa tcttccacac
     2101 tctctgagta acgctttaag caaagcgcta ccaaaggcag tttctgtaga ttttttgcaa
     2161 gagaggttcc gttaggagtt tgctttaaca gccagctcga tgtcgctctt gggtgatcaa
     2221 ggctactatc tagacgcttt atgattctta atagttgtcg tacagcaatt cgatctcttt
     2281 ggttccaatc aactctaggg gcgggtgcca gacgctcttg ttgatgctgt tgattccagt
     2341 gaactagcca atccctgtca tgtcggtaaa gccaagcgta tagcacccca ccctctaaag
     2401 actgtcttgc cgccttaatt ccttggtatt tatgcactag ttgctgccag tctttatgct
     2461 taacagataa atcttcagag ttaggttgca cagactggct aacaggtctt gtcgttatag
     2521 agtgctcagt aagagcactt gcctgctgta gcgcttctat aaccgttagt tttggcaata
     2581 aggcttgcca cacaatacta tgctgtaaat aactaaaggc ttttctatgc ttacggaata
     2641 tacttttcag ccaacacgtg tccttgttct ctgccaactt taaatccagt ttctctagtg
     2701 cctcatcact aaaagtctgc ctcactctct ccgccaccaa gtcatgacga atgtgcttgc
     2761 ttttggttag ccctagatcc tgcgctaagc gctgataaaa cagcgtccac tgctcaaggc
     2821 ttggggaaag ctcttgcgct cgtggagcat ctaacagagg ggctatataa gcagctagtg
     2881 ctgttaattg agatagggag tctttggggt agtctgaaag cagctcagta tgacccaaag
     2941 cccaaaattg atgtcggtga tcatctacag ctctatcaaa gaagactaaa gcaccgtgtt
     3001 ttggacaata tggcaaagcg ggcaaatacc aatctcgttg ccaaaaggct tccccatacc
     3061 tatttagctg aagagcaacg caatcagggc agtatctaaa gcggttatcg ctcttaactc
     3121 tagaagcagc gactcctagc attaaatgca ccgcaccttg cgcttggtac tccattaacc
     3181 gaatagcttc gtctcggcgc tccttgccta caaacggagc atataaaggg aataaggtat
     3241 gctcataaat aagctgctga acagcgtaac gtcctgtttg atgtagatgt cttgctatca
     3301 cacctaaatg cgagggcaga cctaaggtag cgaccacctt gcggttgcca tacacctcat
     3361 ccaacagctg cttaggacta acaatccctt gataaacgcc tgcccgtgca atagtgctat
     3421 aaatcagctc attcgagtac ggaacaggaa agtttctcat ggctaacctg cttgtttaaa
     3481 taagctcgcc atatccacta ttaccccttt cccttttaac cgttcatgca tggttttttc
     3541 aggttggcgt tgtgaataga tataacgtaa atccgtatca ggcaagctat cccagtcgct
     3601 gggcttgact acctttatag ccgaaacctt tttactcttg gagggctttt ctagttctga
     3661 cactaccgtt tcgccttcca tcaaccactg caaaacaaga ggcagtaact tttgtcttgt
     3721 catcgttgga ttctggctaa acgctttttt aatagtggga attaacaggc ttgaatcgta
     3781 atcctctttc agcatcagat ataaatgacg ctgatcttcg gtatctaact cttgaagggc
     3841 tttttcttct ggtgtttgtt cttgtatcgc tgcgatatct agctgaagtt ggattaaccg
     3901 tttatcaatc tcgggaacga ctagatcaga ataacgagca atgcgttctg ggatacccga
     3961 gcgtaatgcc tctagcatgg ggtgcacagg ctttaactca tcttgataca cttgccgcaa
     4021 taaaccagcg gtaatacgct cattgcctaa agctagcgca cggagctgag cgagtacaaa
     4081 aagttttact acaatgtcca tcactccttg gcttagctca taccacacat cacggacctc
     4141 atccgataac agcgcatctt tgcgttgtaa aagctgtaat tgccaaagat tatccgtaaa
     4201 agcgatccac tcttgattgg gctttccacg ttgcgtttgt tgtataggat cccagaatat
     4261 agctccaaac cctgcccctc tacgtgcaga ccgcaaatca gcctcaaaaa tctctcgtgc
     4321 tttaggggta ccaatcaaca tcactggtac gccaataata ttcaccatcg tcacaaaaaa
     4381 gttcagcatc tcttgagatc cacccgaacg agagcggctt aaatgctgaa tttcatcaat
     4441 aaccaacaac cctaaagcat gtgcattggc tatttgcgac atcaaagcca acatggtttc
     4501 tataccatga cgttttaagc cataacgacg ctcatagttc gagcccaagg ctcgatccaa
     4561 cgctctgaaa aaattcaagc agatttcttt tagcgaacca ttatgcgagc agtctatttt
     4621 caaatacacc acctgctcta cattgagttc acgatggtaa atcacctgag gatacgtggc
     4681 tagaatacaa tgaagagagg tcgtcttccc actaccagaa caaccaatta acaataagct
     4741 ttgtgccgta gatcgtgcct cctcaaagcg aaatgtctcc aactctcccg tttgaacacg
     4801 ctcataacca ttttgtaaat gcttttgtaa atctcctgtt ttaggatttc tgcctacgta
     4861 gccacctcga atcatgaccg aaatacgctc acttagtagc aaatgcgtac ctaatggctg
     4921 aaaatagtca tctggaatac gacaaatggt atgagctctg ataacacggg acttttgcaa
     4981 gtcagaggaa gtaagctgta aagaggattt cagtgatgca gcactattca ctgactcttg
     5041 taatggtggt aaggcctcga taaaaggatt atcacgataa gcctctaccc ccgtatcacg
     5101 ataaactgct tgaatccggg tagcactcat gactcatcct tttctggtgg atcctgaaat
     5161 aattcaggca cgtatgtggg taggctgtaa tcttcttgat catccgcttc cactgcgttg
     5221 aaaggaataa ctttagcctc atcacctgaa gagcttggct tcaaatgctc cgcacgtttt
     5281 ttacgctccg aggtcacggc ttctttttta ttagttttaa tctgcttaat gcgtgttgat
     5341 ttgggctcag tagtactggg cgttaactta ttcgctttct gaatggtttg ctgaataaac
     5401 gcctcaagct ccctgcgttt agttaactca tcctgcttcg cattggcttt attgtgtttt
     5461 tcttgtgctt gtatatccca aacctcccaa aatgagagac ctttaaactg ccgactacgt
     5521 tccgtcagat tacagcgcca aaatacacgg ctgccaactt gcggaaacaa ataaatcgta
     5581 tcaaccagca ctgggtcata agccgcttct aaatgttgag gtctagctat atcagtgctc
     5641 cgctgcaacc aaccctcacg cagaatctct gaccccgagt aatacaaacc ccacaaatta
     5701 acgccaaatg aagaaataga gacctttcgg cgaggcagta acgctactcg caactgctct
     5761 tgctccacag cccttaaact acctgtacga tgctgcatac cccattgcca tagctggaca
     5821 ggaatagacg gtaaatctgt aggaaaatca gcatctcgat cgtatttatc catcaccaga
     5881 tggttatttc tgaataagat cgtacgcaaa ataatttgtg tgaactcaaa taccgacaga
     5941 gatgcatcta acctatagtc tgtttcacca tggcttttga tccgactgcc ctctacaatg
     6001 ccaggtgcaa aggacttaaa ctcggcttgt agtgttctaa aagtgctttc cactatgcct
     6061 ttagcatcgc cacgtctagg tggagcactt tccactcgca cattaaaact agaaactaag
     6121 gcttcgacct gatgactcat taattcgcca cggtccgcta gcaacacatc tggcaaacct
     6181 acacacggcc agtctgagct actaatctca atatcatgct gggcacaaat ggccgttttg
     6241 tcagagcaag catttacaaa agcctgcatc gccaccacat aagacggatt ttcaaagccg
     6301 atataaaagc ccgtgatcat ccgactaaac acatcaatca caatgtaaag cgttggtctt
     6361 cctatgattt tttggcgatc atgatgatcc actaaataaa tatcagcaat cgtggcatca
     6421 atctcataac gactcccagg gcctaacgcc tgagaagtgg ctgtactact taagggtcgt
     6481 acgtcttttt tatatacccc tgctttaact ctagacttta agcgctgagc tttagggtat
     6541 tctcgatcat aaaaataacg aaactgacgt agtgttgggt aatcctcttg gggaatgcga
     6601 ggaaaatact gagcaaacaa gtccacaaat cgtctatagg caacggtggt ctttgtacct
     6661 ttttgattta acaggtgctt ttctatggtc aacctaaaaa ggcgttcaat ctcgggcgtt
     6721 accttggttc cttcaccctt accatattct ctggctcggc caatctttgc tgttcctgtc
     6781 gctgaacgtc tttcccctgg tgcaccgctg tttttgtagt caggaattaa tgcattaggc
     6841 gtttgaccac gctgccagta acggcgtaac aacttataaa ctgtagcctt agtaacctta
     6901 tgttcttgga ccacatgctc aacgagttcg cttctgactt tagggtcgaa acgatcctta
     6961 ctattaataa taggaagaat ttttcgataa tcctcctcgc gcttctggaa gctgacagaa
     7021 tctacagacg gctcttctaa atcaagatgt acataagggt catcagttct aactaagcgg
     7081 ccttcatcta aatactgcat tagcaacaac tcagccctag cttgtggaac tcctttatca
     7141 gcgcttattt gcatccagac aacttggcca tcctctatag ccaaaatgcg atacggatca
     7201 ttatcaaata gcacaacctc attaatttgc cacatagcgc aactcctcca tattcactac
     7261 ttggctaata cagagatctg cacacttatt tgccctgaaa gacttataaa tattgaactt
     7321 aataaaacca tttgcggtta aggctcgtat ctcactcaat gttttgccta actccaaatc
     7381 ataagcaata tcaacctgct tacagacatt gataatgttt tcatctcctt tttcttgcag
     7441 gatatgggcc aatggggata gttgtgctaa aagctccgca gaaacttctt ctgttttcac
     7501 tgaataaagc cattcaatat tttcttttac tacgggattt atttctttat cagtaaaaat
     7561 gaaccaagga atttgctttt gctgccaata gcgacgctct agttctagtt tttctaaggt
     7621 acgctcgtct tgtaaggctg ctgcaggttt gacttgaata gcaaactgct caaaaggacc
     7681 atctttgcag tccactaaaa aatcagtaga cataacctga tctacaccac gaataacagg
     7741 atgcttaata ccactatcta ttgcaatctg cctggtatca ctaggtaata aggggaactg
     7801 ctcgcgtata tctagcacgc tgctctccca ctcaagactg agaaaaacag caagctctaa
     7861 gtcagatagc aaatgatgga ctcgtcccgt cttatgagaa taaatacggt gggaacgacc
     7921 tgaagaagga acttcttgta ctgttagcca tggaatatag tctttaccat gcccttggcc
     7981 acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt ttgctttagc
     8041 cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca gtttacaact
     8101 attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc agtttaagac
     8161 tttattgtcc gcccacaagc ccttttttat ctatgatagt gattactcaa ccgtaaccga
     8221 ttttgccaag ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc
     8281 cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat
     8341 ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata
     8401 caactgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc
     8461 gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg
     8521 tttcagctcg ccagcagcgt aggcttcagc gtgaatgtag gagatctctt tcagcttcaa
     8581 tgcgccttcc agtgcgattg ggtactgatc gccacggccc aggaacagtg cgtgatgttt
     8641 gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc tgagatagca tctgctcaat
     8701 acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc
     8761 tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc
     8821 tttagtggac gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc
     8881 gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc
     8941 tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat
     9001 catcaggctg ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac
     9061 gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata
     9121 agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt
     9181 cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgcccagtaa gggtgttttt
     9241 gatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc
     9301 gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt
     9361 atcgaagatg tttaccgaac ggcgagtaat ttccgcaata tcgccctctt caaggaagat
     9421 aaagcgacgg gtcaccggca gcagcgccag ctggtcagaa gcgataaagt tttcgcccat
     9481 acccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat ccgggtgacg
     9541 ggagtccatg atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc
     9601 ctcacgcaga gtcccgcctt gttttagctc ccagttcacc agatgggcaa tcacttcggt
     9661 gtcggtttca gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg
     9721 gttttcgatg atgccgttat gcaccaccat aatgtgttca gaaacatgcg gatgcgcgtt
     9781 agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg
     9841 cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg
     9901 ggtcatatga ccttccgcat caacaacggc cagaccagca gagtcatatc cgcggtattc
     9961 cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac
    10021 aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt
    10081 tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg
    10141 gagggagatt atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt
    10201 ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat
    10261 gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct
    10321 actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg
    10381 gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg
    10441 ttatcgccaa tttccgcatc gcccaggtaa gtcagatgac cagctttcga gcctttaccc
    10501 agacgcgctt ttttcatctc aacaaagtta ccgacgtgtg caccttccag caactcagca
    10561 ccaggacgca gacgggcaaa cgggccaata gtacaggccg ctgccagatt cacgtcttcc
    10621 acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa
    10681 ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta
    10741 tcaatttcaa catcgcaccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg
    10801 cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc
    10861 agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga
    10921 acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat
    10981 tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatatc tgcgccgtta
    11041 gagatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg
    11101 tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga
    11161 tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg gtttagcatc acgcaggcgc
    11221 tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca
    11281 tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga
    11341 agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg
    11401 tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc
    11461 atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct
    11521 ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta
    11581 atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa
    11641 tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg
    11701 accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg
    11761 tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg tgttttatat
    11821 cagcaggatc tatgtgaacg ctcttcagga cggatcacgc gtaaaaaaaa accagcctgt
    11881 ttccagactg gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg gtcaactcga
    11941 taacgcgcag ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg taatctacgt
    12001 cgccgtgaga gctgctaatg tgctcttcag ccttacgttt cgcttccatg gctcgcgctt
    12061 cgtcgagatc ctggccgcga attgcggtgt cggccagaac ggtcacgttg ccagactgca
    12121 cttcaagaat gccgccagac agatagataa actcttcgtg accgtgctgt ttcacgatgc
    12181 gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg gccagggtag atccccagtt
    12241 caccttcgct acccgttacc tggattttct cgaccagacc agagaacatt tgttgctctg
    12301 cgctgacgac gtccaggtgg taagtcattg ccatatcacc ctccgattaa ggcgttaaag
    12361 ttttttggct ttttccacag cttcttcgat ggaaccgacc atgtagaacg cctgctccgg
    12421 caggtgatcg tattcgcctt ccatgatgcc tttaaagcca cggatggtgt ctttcaggga
    12481 gacgtattta cccggagaac cggtgaatac ttctgccacg aagaacggct gggacaggaa
    12541 gcgctggatc ttacgagcac gcgctaccac cagtttgtct tcttcagaca gttcatccat
    12601 acccaggatg gcgatgatgt ctttcagttc ctgataacgt tgcaggatgg actgaacgcc
    12661 acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg tccagctgac ggctggtgga
    12721 gtccagcggg tcaacggccg ggtagatacc cagagacgcg atctgacggc tcagtaccac
    12781 ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt catccgcagg
    12841 tacgtatacc gcctgtacgg aggtgataga accagttttg gtggaggtga tacgttcctg
    12901 cagaacgccc atctcttccg ccagggtcgg ctgataacct accgctgaag gcatacggcc
    12961 cagcagtgcg gatacttccg taccggccag ggtgtaacga tagatgttgt caacgaacag
    13021 cagaacgtca cgaccttcgt cacggaattt ctcagccatg gtcagaccgg tcagagcaac
    13081 gcgcagacgg tttcccggcg gctcgttcat ctggccatac accagggata ctttgtcgat
    13141 aacgttggag tcggtcattt cgtggtagaa gtcgttaccc tcacgagtac gttcacctac
    13201 gcccgcaaac acagagtaac cggagtgctc gatcgcgatg ttacgaatga gctccatcat
    13261 gtttacggtt ttacctacac ccgcaccacc gaacagacca actttaccgc ccttagcgaa
    13321 cggacacatc aggtcgataa ctttgatacc ggtttccagc agttcctgag agtttgacag
    13381 ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc
    13441 tttcatgtcg accggttcac ccagtacgtt catgatacgg cccagagtcg ctttacctac
    13501 cgggacttcg atcgggtgtt cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga
    13561 ggaacccatt gcgatggtac gcacgatacc gccgccgagc tgctgctgaa cttccagcac
    13621 cagacgctca ttaccatttt gcacctcaag agcatcgtac acgcgcggta cggcatcctg
    13681 agggaattcg acgtcaacta cggcgccgat tacctggaca atctttccag tagccatctt
    13741 aaatcctcta cgaaataacc tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt
    13801 tcctgagtaa tgctggcctg acgagctttg ttgtatacca actgcagctc tttaatcagg
    13861 ctgccgccat tgtcggtcgc ggctttcatc gccaccatac gggcggcctg ctcgctggcc
    13921 aggttttcaa ccacgccctg ataaacctga gattcgacat aacgacgcag cagggtatcc
    13981 agcaacgcct tcggatcggg ttcgtacagg taatcccagg atttatgttt cagatcatca
    14041 tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga catggtgtta
    14101 ataaatttgt tgctgacaat gtaaagcttg tccagacggc cttcgtcgta ggcctgcaac
    14161 atcactttta ccggaccgat cagttcggac agggaagggt tatcccccat gccggtgacc
    14221 tgggcaacaa cattgccgcc cacggagttg aagaacgaca cgcctttcga gccgatcatt
    14281 gcgaggtcgc attgaacgcc tttgtcggtc caggtcttca tttccgccag cagttttttg
    14341 aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac caggtagccc
    14401 acgcgtttaa cgtcgcggtc ttccaggtaa aggtgcttat attccagatt accatgtgca
    14461 aggtgaccaa tcactttgcg catggtttct gcataaggac ggctggccgc catgcgatcc
    14521 tgcgatttac gcattttgga agcggcgacc atctccatcg ctttagtgat cttttgcgtg
    14581 ttctggacgc ttgcgatctt actacgtatc tcttttgcgc cggccatgag cttctcctca
    14641 atgccttgcg gcctgcccta aggcaagccg ccagacgtta ccaggattgg gttgctttga
    14701 aggaatcgag gatgcctttc agtttgcctt cgatttcgtc gttgtagcca ccggtctggt
    14761 tgatctcttg catcaacgga gcgtgatcac ggtcgacgta agccagcaga gcggcttcga
    14821 agctgccaat tttcgacagt tcaacatccg ccaggtaacc acgttctgct gcgaacagaa
    14881 ccagagactg ctgcgcaacg gacatcggcg catactgttt ctgtttcagc agttcggtca
    14941 ctttctgacc gtggtcaagc tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag
    15001 agaacgctgc cagttcacga tactgtgcca gagcggtacg gataccaccg gacagttttt
    15061 tcatgatctt ggtctgtgct gcaccaccaa cacgggatac ggaaataccc gggttaaccg
    15121 caggacgaat accggcgttg aacaggttgg tttccaggaa gatctgacca tcggtaatgg
    15181 agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc ctgagtttcg ataatcggca
    15241 gtgcggtcag agaaccggtt ttccctttca cttcaccttt ggtgaaggct tcaacgtatt
    15301 cggcgttaac acgtgcagca cgctccagca gacgagagtg gaggtagaaa acgtcgcccg
    15361 ggaatgcttc acgtcctggc ggacgacgga gcagcaggga gatctgacgg taagcaacag
    15421 cctgtttaga caggtcatcg taaatgatca gcgcatcttc accgcggtca cggaagtatt
    15481 cgcccattgc gcaaccggca tacggtgcca ggtattgcag tgcagcggat tcagacgcgg
    15541 ttgctaccac aacgatggtg ttagccagtg cgccgtgctc ttccagttta cgtaccacgt
    15601 tagaaatggt ggacgctttc tggccgatag cgacatagat acatttgata ccggaatcgc
    15661 gctggttgat gatggcatcg atagccagtg cggttttacc tgtctgacgg tcaccgatga
    15721 tcaattcacg ctgaccacga ccgattggga tcatggagtc aacggcttta taaccggtct
    15781 gtaccggctg atctacggac tgacgttcga taacgcccgg agcgattgct tctacagcag
    15841 agaagccgtc gtgatccagc ggacctttac cgtcgattgg tgcacccaga gtgttaacca
    15901 cacggcccag caggccacgg ccaaccggaa cttccaggat acggccagta cacttaactt
    15961 tcatgccttc ggcaaggtca gcgtacggac ccataacaac cgcacctaca gagtcgcgct
    16021 cgaggttcag tgcgatagcg taacggttac ccggcaggga gatcatttca ccctgcatac
    16081 aatcggccag gccgtgaatg cggataacac cgtcacttac agaaacaata gtaccttcgt
    16141 tgtgagcttc actcacaaca ttgaactgag caatgcgctg cttgatcagt tcgctgattt
    16201 cggtggaatt cagttgcatg ctccagtccc cttaagactg caagacgtct gcaaggcgct
    16261 caagacgacc gcgtacgctg ccatcaatga ccatatcacc cgctcggatg ataacgcctg
    16321 ccattacaga cttatcgatt ttgcaattca gcttaacttt gcgtgacaga cgtttttcca
    16381 tcgcagcaga aattttcgcg agctgttgtt cactcagtgc ggcagcggaa atgacgtcta
    16441 cctcagcggt agcctcactc acggcacgaa ggtgaataaa ctgctccaga acatccggga
    16501 gcgcgttaag acgaccattt tcagccatta cccgaatcag gttctgaccg ttttcgtcca
    16561 gttgctcacc acaaactgcg ataaacgact cggcgagcgt ttctggcgca agcgcgccag
    16621 agagaagctc tgccatttgt tcgtttttgg ttacctcggc ggcaaacgcc agcatgtcct
    16681 gccagcgttc tacactttgg tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc
    16741 gagctaccgt aataaattca gacatcagcc cctccctcct tacagttcag cgacaagttt
    16801 atccacgatg tcgctgttag cagcttcatc cacggaacgt tcgatgatct tctcggcgcc
    16861 agcaacagcc aggatagcaa cttgcttacg cagctcttca cgggcacgtt tacgctcggc
    16921 ttcaatttct gcctgcgcct gggccacgat tttagtacgt tcctgttctg cctcagcttt
    16981 cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc tcgatgatta cctgggcttc
    17041 cgctttcgct tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg
    17101 tgctcgttct gcggaagcaa ggccgtcagc aatttctttt tgacgttttt cgatggctgc
    17161 cattaatggc ggccatacgt acttcatgca gaacagaacg aacaggacaa acgcgatggc
    17221 ctggccgagg attgttgcgt taagattcac agcacaatgc ctctatttag ttaacgttct
    17281 gatattgctc tttaaataaa agcaacgctt actacgcgac agcgaacatc acgtacagac
    17341 ccagacctac agcgatcatc gggatagcat ccaccagacc cataacgata aagaactgag
    17401 tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc ttccaagaat ttacccccga
    17461 ggatgccgat accgatcgca gcaccgattg ccgccagacc catcatcaca gcggcagcca
    17521 tgtacagcag atccatattc aggttttcca tgacagtctc cagtttgttt cagttaaaac
    17581 gtagtagtgt tggtaaatta atgttcttca gacgccatcg acagatagac gatcgtcaga
    17641 accatgaaga tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca
    17701 ttcaggatcc actgtgacca ccacggcaac agaccagcaa tcagaatgaa aatcagctca
    17761 ccggcataca tgttaccgaa cagtcgcaaa ccgagtgaaa ctggtttgga cagcaggctt
    17821 accccttcaa ggattaagtt gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc
    17881 aactctttcg tgaagccgcc gatgcctttc attttgatgc tgtagaacag aatcaggata
    17941 aatacgccca atgccataga cagcgttacg ttcacgtccg cagacggaac cacacgcagt
    18001 gcaggcagac ccagtacatg ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc
    18061 atcaggttca tcaggaatac ccagacgaag atcgtcaggg ccagcggagc aatcagcttg
    18121 cttttgccat ggtacatgtc tttcacgcta ccattaacaa agccgatcac cagctcaatc
    18181 gcggtctgaa acttacctgg cacaccgctg gtcgcctttt tggctacgct acggaataaa
    18241 accaggaaca acagacccag caccaccgag aagaacatgg agtcaatatt gattgtccag
    18301 aaggtggctg gggggttttg tggatccacc agcgagaatg tacgcaggtc cagctgaagg
    18361 ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc catgatgcct
    18421 tttacccttt gttgttaatt acagccggtg ccagtatctg aaccaccagc accaaaaccc
    18481 acgtaacgat cagcggcaag aataccgcct ttaaaaccgc caacgccacc accagtaaca
    18541 ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa tgtccaggcc acccggcctt
    18601 tcgctggtgt atgcgcctgg tgacgccagg caaatatcat aaacaaaacg ttaggcagaa
    18661 agactgccag gcccccgctt attgcagaga cgccccagaa ggggtctttg aggctgaaca
    18721 gcaatccact tgctatcacc accagtaact gaacgagcag aagcttccga gcaacgtttc
    18781 gactcacgag cgacacagac atcacgtttt ttactcctgc tcccttcgag gtatgccgcg
    18841 tgtcgtataa aactttcttt aaggcttaga gtcaagcatc aaaaagcggt caaattatac
    18901 ggcgcgcccc cgtgatttca aacaataagt agccaaaagg tgaataaatg tttaaatatt
    18961 tttccagtgc atacaattgc gacttttcta ctaaccctgt tcgatcatga aaaactgtaa
    19021 ataacgcgta aacactggtg ataaagcgtg cttcagatca catattgcgc atgttcgcgc
    19081 acagcatatt tatttacttg gcaaatgatg cctttgcaag tttatgatat ttcagtctaa
    19141 aaacagatac tgttttaata aatgacattt acacaacaaa aaccacccat tgacattttt
    19201 aataatgttt tagcagccaa tgatggttct tagcgccgat ttttagcaga ctgatatttt
    19261 cactaatgac ttattttctg cttaccaaaa aaagccacgt tatcttgttg atgcaaaaga
    19321 gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa aacctggttg ttgttaacag
    19381 tctaaccggt caatttttta tgattttttt gataaaaatt aaattttatt tgctttaatc
    19441 accaccagat gacgttcgcc atccagggct ggaacctgaa gtttaaccac tgattcgacc
    19501 tgatattctt cgggcaacaa agcgatttca tcttccggca tttgcccttt cagcgcgtag
    19561 aaacggcctt gctcaccagg aagatggtgg caccagctca ccatatcgtt cagagaggca
    19621 aaagcgcggc taattacgcc atcaaatggc ggctctgaag gaaactcttc taccctgctc
    19681 tgtacaggtt caatattctc cagtttaagc tcatgttgca cctgacgaag gaaacgcacg
    19741 cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag gacgcacgat agagagtgga
    19801 atgcctggca gtcctggtcc ggtgccgaca tcgataaacc gttcaccttg cagatacggt
    19861 gccaccacaa tgctatcgag aatatggcgt accagcatct cattaggatc gcggaccgaa
    19921 gtcaggttgt acgctttgtt ccatttatgc agcatattca cgtaggcaat aagctggttt
    19981 ttctggtgat cggtaagcga aatacctgcg tctttcagca gtaaggagag tttgttgagc
    20041 acggtgatta cctgttcttg atgcgttgcc tggtaagcgg gtgcttacca ggcattttta
    20101 atgcgttatg cgctacgacg cagcataccc tgttttttca gccacaccag cagaatggag
    20161 atagccgcag gcgtgacgcc agaaatacgc gaagcttggc cgatagaggc tggtttgtga
    20221 tcgttaagtt tggcgatcac ttcgttagaa agaccggata cctggcggta atccagtgtc
    20281 gcgggtagca gggtgttctc gttacgcagc tgcttttcga tctcatcttg ctggcgcgcg
    20341 atataacctt cgtatttaac ctgaatctca acctgttccg ccgcctgttc gtctgtcaac
    20401 gcaggggcaa acggcgtcag cgtggttaat ttttcataag tcatttccgg acgacgcagc
    20461 agatcttcac cactggcttc acgggaaagc ggcgcagtca ggtgagcatt cacttcggct
    20521 gcagcttccg ccgacggggt tacccaggtc gatttcagac gctgacgctc acgctcgata
    20581 ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat ccaccaggcc cagttcacga
    20641 ccgatttcag tcaaacgcag atccgcatta tcttcgcgta gcatcagacg atattctgcg
    20701 cgcgaagtaa acatacgata cggttctttg gttcctaaag tgcacaggtc atcaactagt
    20761 acgccgagat acgcctgaga acgtgccgga gcccaacctt ctttgtcagc agacagacgg
    20821 gcagcgttaa gaccggccag caaaccttgc gcagcggctt cttcgtaacc ggtagtgccg
    20881 ttaatctgac cagcaaagaa cagcccctgg ataaacttgc tctccagcgt cggtttcagg
    20941 tcgcgaggat cgaagaagtc atactcaatg gcataacccg gacgcacgat cttcgcgttt
    21001 tccatcccct gcatagagcg gacgatttgc atctgcacat cgaacggcag gctggtggag
    21061 ataccgttcg gataaatttc attagaggtc agtccttccg gttcaaggaa gatctgatgc
    21121 tgatttctgt cggcgaagcg catgactttg tcttcgatcg acgggcagta gcgtgggccg
    21181 acaccttcga tcacccctgc atacattggg ctacgatcga ggttactgcg gatcacatca
    21241 tgggttttct cgttggtatg agtgatataa cacggcacct gctggggatg ctgggacgca
    21301 ttgcccataa acgagaatac cggcattggg ttatcgccat gctgttgcgc cagcacacta
    21361 aagtcgatag ttcgagcatc aatacgcggt ggtgtcccgg ttttcagacg accaacgcgc
    21421 agcggcagtt cacgcaaacg gcgagaaagc ggaatggacg gcggatcacc agcacggcca
    21481 ccgctgtaat tatccagacc gatatgaatt ttaccgtcga ggaacgtccc aacggtgagc
    21541 acgacggctt tggcacggaa cttcagtccc atttgggtaa cagcaccgac cacgcgatcg
    21601 ttttcgacaa taagatcttc aaccgcctgc tggaagatca tcaggttcgg ttggttctcc
    21661 agcgccgtac gtaccgcctg acggtagagc acacgatccg cctgagctcg ggtagcgcga
    21721 accgccggtc ctttgcttgc gtttagtatc ctaaactgga tacccgcttg atcgatcgct
    21781 ttcgccatca gaccgccgag tgcatccact tcttttacca gatgtccctt cccaataccg
    21841 ccgatcgccg ggttgcagct catctgcccc agagtgtcga tattgtgtgt caaaagcaga
    21901 gtctgttgac ccatacgcgc cgcggccatc gcggcctcgg tgcctgcatg acccccgcca
    21961 atgatgatga cgtcaaaagg atccggataa aacatggtga ttgcctcgca taacgcggta
    22021 tgaaaatgga ttgaagcccg ggccgtggat tctactcaac tttgtcggct tgagaaagac
    22081 ctgggatcct gggtattaaa aaggagatct atttatttag agatctgttc tattgtgatc
    22141 tcttattagg atcgcactgc cctgtggata acaaggatcc ggcttttaag atcaacaacc
    22201 tggaaaggat cattaactgt gaatgatcgg tgatcctgga ccgtataagc tgggatcaga
    22261 atgaggggtt atacacaact caaaaactga acaaccgttg ttccttggat aactaccggt
    22321 tgaaccaagc ttcctgacag agttatccac agtagaacgc acgatctgta tacttatttg
    22381 agtaaattaa tccacgatcc cagccattct tctgccggat cttccggaat gtcgtgatca
    22441 agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac cggaattttt gagttctgcc
    22501 tcgagtttat cgatagcccc acaaaaggtg tcatattcac gactaccaat accgattgcg
    22561 ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca atgcttcata gaaaggagaa
    22621 aggttgtccg gaatatctcc ggcaccgtgg gtggagctga taatcagcca gatccccgag
    22681 gcagataaat cttccaacag cggaccgtgc agcgtttcgg tggtaaaacc cgcctcttcc
    22741 agcttttcag ccaggtgttc tgctacatat tcggcaccgc cgagggtgct gccgctgata
    22801 agagtgatat ctgccataaa ccgccacctt tattaagagt ggcgtattgt acgctgtgaa
    22861 cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa agccgatcag ggcttgatag
    22921 tacgcatgat cgggtgttgc aggacgatca atgtctcggt ggactgaatt tcatcaattg
    22981 tttggatctt gttgataagt acatgctgga gagcgtcgat cgaacggcac attactttta
    23041 taaagatgct gtagtggcct gttgtgtaat aggcttcagt gacttcatca aggctttcca
    23101 gctttgccag cgcggaaggg tagtctttgg cgctctttaa tataatgccg ataaagcagc
    23161 ctacgtcata accgagctgc ttcgggctga catcaatacg cgccccggta atgatccccg
    23221 cctgcttcat tttctctacc cgaacgtgaa tcgtccccgg actgacgcca aattgtttcg
    23281 ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc ttccaggatg ccacggtcca
    23341 gattgtcgat cagataattt tccataggat tttcttatgc agattgatga ttcattctat
    23401 tttagccttc ttttttaatg aatcaaaagt gtgttaggct ttttattgaa tgattattgc
    23461 atgtgtgtcg gtttttgttg cttaatcata agcaacagga cgcaggagta taaaaaatga
    23521 aaaccgctta cattgccaaa caacgccaaa ttagcttcgt gaaatctcac ttttctcgtc
    23581 aactggaaga acgtctgggg ctgatcgaag tccaggcacc gattcttagc cgtgtggggg
    23641 atggcacgca ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa gtgaaagctc
    23701 tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa gtggaaacgt cagaccttag
    23761 ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc cttcgccccg
    23821 atgaagaccg tctttctccg ttgcactcgg tctatgtaga ccagtgggac tgggaacgcg
    23881 taatgggcga cggtgagcgt caattctcga ctctgaaaag cacggtagag gcgatctggg
    23941 cgggaattaa agcaaccgaa gctgcggtta gcgaagagtt tggcctggca ccgttcctgc
    24001 cggatcagat ccacttcgta cacagccagg agttactgtc tcgttatctg gatctcgatg
    24061 ccaaagggcg tgagcgggcg atagcgaaag atcttggcgc ggtattcctt gtcgggattg
    24121 gcggcaagct gagcgatggt catcgccacg acgtgcgcgc accggattat gatgactgga
    24181 gcaccccgtc agagctgggt catgcgggtc tgaacggcga tattctggtg tggaaccctg
    24241 tactggaaga tgcgtttgag ctttcttcca tggggatccg cgtggatgcc gacacgctga
    24301 agcatcagct ggcgctgacc ggtgatgaag atcgtctgca gctggaatgg catcaggcgc
    24361 tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat cggccagtct cgtttgacta
    24421 tgctgctgct gcaactgccg catatcggcc aggttcagtg tggagtatgg ccagctgctg
    24481 ttcgcgagag cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc tgagcaggcg
    24541 gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa atgcgcatga tgccgggttt
    24601 gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga tgtacccgct gcagctcttt
    24661 cactttactc gtcacgtcgt caggcaaccg ctgagcgata aaatcagaaa tcaccaccgc
    24721 atcggcatca aaccattccc tgctttgcaa gcgttccata atggcgcgaa aacaactggc
    24781 aagatcggtg ccgccacgaa actgctggct taaaaaacgg attgcttgtt cgatgccttg
    24841 tgggcctgaa agctcataac ggacgatctc ggtggaaaat agcataatat agcagcgccg
    24901 gttttctgcg agagcaatgc gcatcaaggc caggcagaac gctttcgcac actgttcatt
    24961 aaagccgccc attgagccgg aagtatccac acagacaata aacggcccgc gcggctgttc
    25021 atcgtaatct ttatgtacca ccggacgttc gatcactttt tcacgccacg actcaccgtg
    25081 caggcgatag gtgagcaact gtttttccac cagccgacgg taaaactcat actccagttc
    25141 cgttatccct agtgtcgcca gttctggcgg caggagacgt aaaatatcat cgctttgttg
    25201 cagaccatca acctgctcag gaaccgtcgc cggttcgcgc accatggtgc ggaaggtttc
    25261 catctgcgca tcgttgcgcg gtattgattt ggcttcccga gaacgcccca gctgctctgc
    25321 caggcgtttc agttccggct gttcgttaag aaattcaccg tatttcacaa tcaactgata
    25381 gtcgccacgt ttaagctggc cggcgctcat atcccacaga cgaccagctg cggtattgtt
    25441 atctgcgaga atcggttcaa gttgtccgct cagcgtcatg cgttcctgaa cttcacttaa
    25501 cagttgttcg cgttcttctt ctaatagctg ttgatttaac gtcgttgctt gcacgatcag
    25561 acttaaacgc cagcgctgga gaaaaagcgt gtgtaacgct gaagttattg tgctgttagc
    25621 atcaaccaac tgccgggctt gttctgccca tggggaattc agacgatgca gtaagtccag
    25681 gatctgtggt agctgcacaa taaactgcgg cgtggagagg agctggcttt gctgatagca
    25741 catcacctct tcggtgagtt ctggcgggac tcgggcatct ttcagccgac tgcgcagcgc
    25801 ctcacgccag cggggaacat catcagtgat tgctgccttc agtcgtggga atttttcaaa
    25861 gaagaccgcc agctgcggtg aggccagcag tgcgatgatc atctcttcga tcaatccctc
    25921 ttcgctgacg gccagcatca cattaagcgt atccagcgtt agcattgttg tgcctggcga
    25981 atctgtgcac cgacatcctg taggctggct tcaatgcgac ctaaccagtc gccagggata
    26041 aacagacatt tttgctgttc gctgaataaa gcgtgttgct tacgccagtc gctttcaagc
    26101 tcttccagtt gttgttttat ttcacctggc agaccttcag ccgatgaacc gggaagtgcc
    26161 agcgtactgc cttgtaaact tacatcgcgt acaacaagat gttgggtgct atcaacttcc
    26221 agattcagtt tctgggcaaa gccgataccg ttcagtttgc cgcgaatttc accaccttta
    26281 ctcagccact gttccagcgc gctacgctca aaggagatat gaaccacttc catatcatgc
    26341 agttttaacg gtttttgcag cagcagagtc agagtggaag cagtaacgtt aacagggagt
    26401 tgatactgct gacgacggct aaaaatgccg cccagacgaa ttaccgttaa ggctgtttta
    26461 tcgctttgtt gctgctgaag ttgcaggtga cgttgcacaa tcgcgcctag gcgggtcaac
    26521 atcccttgct gttgccaggc gtgaccggtc atcaatacat caatttgttg ttgtatcaaa
    26581 ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca aaatgagatc taccggggca
    26641 acagcactgc gaccgctaaa aaaggcgctg gcctgcaata atcggatcgc ttttttccag
    26701 cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg cagcataaaa
    26761 atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg ccagcgttca
    26821 tattcttcat ctgtgacctg caaggcatca ggaaccggat tgtcgttttc atcctgttga
    26881 ctggtcagca tggagcggaa attcgcttta tcctgcactt tatctaacca cagacgaatc
    26941 agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc gttggaggcc
    27001 gccaccagca gacgcatcgg gattttttct acgtgtgcac cgttgcggaa ctggcgctcg
    27061 ttaatggcgg tgagcaaggt attaagaatt gccgggcccg ctttccagat ctcatccaga
    27121 aagacaattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg cccttcatct
    27181 tttagcgcct gaatagaaag gggaccaaaa acttcttccg gcgtggagaa gcgggtcatc
    27241 agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc gatcaaactt
    27301 ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc cgctaataaa
    27361 cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact gctcaggcgg
    27421 gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc tttcgtcaaa
    27481 agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata gtctcaaag
//
LOCUS       DADLTD010000032        26767 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLTD010000032 DADLTD010000000
VERSION     DADLTD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.032"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..326)
                     /locus_tag="JN701_001094"
     CDS             complement(<1..326)
                     /locus_tag="JN701_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7363611.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDA"
     gene            complement(523..1296)
                     /gene="hisP"
                     /locus_tag="JN701_001095"
     CDS             complement(523..1296)
                     /gene="hisP"
                     /locus_tag="JN701_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY7363612.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1304..2020)
                     /locus_tag="JN701_001096"
     CDS             complement(1304..2020)
                     /locus_tag="JN701_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7363613.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(2017..2703)
                     /locus_tag="JN701_001097"
     CDS             complement(2017..2703)
                     /locus_tag="JN701_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7363614.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2793..3575)
                     /gene="hisJ"
                     /locus_tag="JN701_001098"
     CDS             complement(2793..3575)
                     /gene="hisJ"
                     /locus_tag="JN701_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY7363615.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3796..4578)
                     /gene="argT"
                     /locus_tag="JN701_001099"
     CDS             complement(3796..4578)
                     /gene="argT"
                     /locus_tag="JN701_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY7363616.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4844..5413)
                     /locus_tag="JN701_001100"
     CDS             complement(4844..5413)
                     /locus_tag="JN701_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7363617.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(5508..7025)
                     /gene="purF"
                     /locus_tag="JN701_001101"
     CDS             complement(5508..7025)
                     /gene="purF"
                     /locus_tag="JN701_001101"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY7363618.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(7062..7550)
                     /gene="cvpA"
                     /locus_tag="JN701_001102"
     CDS             complement(7062..7550)
                     /gene="cvpA"
                     /locus_tag="JN701_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY7363619.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7809..8471)
                     /gene="dedD"
                     /locus_tag="JN701_001103"
     CDS             complement(7809..8471)
                     /gene="dedD"
                     /locus_tag="JN701_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY7363620.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8461..9729)
                     /gene="folC"
                     /locus_tag="JN701_001104"
     CDS             complement(8461..9729)
                     /gene="folC"
                     /locus_tag="JN701_001104"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY7363621.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9799..10713)
                     /gene="accD"
                     /locus_tag="JN701_001105"
     CDS             complement(9799..10713)
                     /gene="accD"
                     /locus_tag="JN701_001105"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY7363622.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10869..11528)
                     /locus_tag="JN701_001106"
     CDS             complement(10869..11528)
                     /locus_tag="JN701_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7363623.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11611..12423)
                     /gene="truA"
                     /locus_tag="JN701_001107"
     CDS             complement(11611..12423)
                     /gene="truA"
                     /locus_tag="JN701_001107"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY7363624.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12423..13436)
                     /locus_tag="JN701_001108"
     CDS             complement(12423..13436)
                     /locus_tag="JN701_001108"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7363625.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQDGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13502..14638)
                     /gene="pdxB"
                     /locus_tag="JN701_001109"
     CDS             complement(13502..14638)
                     /gene="pdxB"
                     /locus_tag="JN701_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY7363626.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14737..15732
                     /locus_tag="JN701_001110"
     CDS             14737..15732
                     /locus_tag="JN701_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY7363627.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15729..16907)
                     /locus_tag="JN701_001111"
     CDS             complement(15729..16907)
                     /locus_tag="JN701_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7363628.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17191..18411)
                     /gene="fabB"
                     /locus_tag="JN701_001112"
     CDS             complement(17191..18411)
                     /gene="fabB"
                     /locus_tag="JN701_001112"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY7363629.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18570..20576
                     /gene="mnmC"
                     /locus_tag="JN701_001113"
     CDS             18570..20576
                     /gene="mnmC"
                     /locus_tag="JN701_001113"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY7363630.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEYPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20697..20975)
                     /locus_tag="JN701_001114"
     CDS             complement(20697..20975)
                     /locus_tag="JN701_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY7363631.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(21009..21557)
                     /locus_tag="JN701_001115"
     CDS             complement(21009..21557)
                     /locus_tag="JN701_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY7363632.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21557..22366)
                     /locus_tag="JN701_001116"
     CDS             complement(21557..22366)
                     /locus_tag="JN701_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY7363633.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22366..23190)
                     /gene="mepA"
                     /locus_tag="JN701_001117"
     CDS             complement(22366..23190)
                     /gene="mepA"
                     /locus_tag="JN701_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY7363634.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23194..24279)
                     /gene="aroC"
                     /locus_tag="JN701_001118"
     CDS             complement(23194..24279)
                     /gene="aroC"
                     /locus_tag="JN701_001118"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY7363635.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24314..25246)
                     /gene="prmB"
                     /locus_tag="JN701_001119"
     CDS             complement(24314..25246)
                     /gene="prmB"
                     /locus_tag="JN701_001119"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7363636.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25412..25963
                     /gene="smrB"
                     /locus_tag="JN701_001120"
     CDS             25412..25963
                     /gene="smrB"
                     /locus_tag="JN701_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY7363637.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(26036..26749)
                     /locus_tag="JN701_001121"
     CDS             complement(26036..26749)
                     /locus_tag="JN701_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY7363638.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6601 a         7310 c         6757 g         6099 t
ORIGIN      
        1 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
       61 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
      121 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
      181 ggttccagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
      241 tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
      301 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
      361 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
      421 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
      481 cccatgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
      541 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
      601 ttcttctatt ttcccctgat ggaggaaaat gacatgagta gaaacatggc gagcaaagcc
      661 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
      721 caacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
      781 ttccggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
      841 atgcaccgga tatttcccct gcgcacgttc gtctatcccg acttttgcca gatacttgac
      901 cgcccgctcg tgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
      961 gttttccagc accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
     1021 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
     1081 caccagattg atcgtctggc cattgaccac gatcgaccct tcactcggtt tttcgaggaa
     1141 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
     1201 atctccggca ttcgcttgca gtgagacccc tttcagcact tcatgttcgc cgtagcgttt
     1261 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
     1321 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
     1381 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
     1441 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag
     1501 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
     1561 aaggcaaaat aatgcaacga tacattttaa aagtcgagaa gccataggct ctggcggctt
     1621 caatttcccc atgcagaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
     1681 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
     1741 ggaattcggt tcccttaaca atctcaagcg tgtatatgcc ggaatagaac accagcaact
     1801 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
     1861 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
     1921 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
     1981 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
     2041 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacagtgg tgaaaaccag
     2101 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
     2161 ttgggtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
     2221 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
     2281 gatccgccga aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct ctatatgtcc
     2341 tttcggcact gccataaaag caccacggaa cgtttcggta aaataagcac cgtagataaa
     2401 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
     2461 ctccgtcacc gtgtttagcg caatctgcaa accgaagaaa atcagcagca tcaacactaa
     2521 gtccggcacg ccacgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
     2581 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
     2641 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatataa
     2701 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
     2761 gaagggagcg ggctttcttt caccgatggc gattagccac cataaacatc aaaatcgaag
     2821 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
     2881 agtgcttcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
     2941 agtttttcat ctttaacaga cgggccaccg aatttgtaat ctttaccgac aggttgtttg
     3001 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
     3061 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
     3121 caatgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
     3181 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccaa acgagaatcg
     3241 gcagcgtaca gtttgtcggt gaaggctatt tcttgctgac gtttttccgt aatggaaagc
     3301 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcgga
     3361 ttttcgacaa acgtacattg cgtattgatg cgtttgcata attcctttgc cagatcgata
     3421 tcgaagccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
     3481 gtaccgatgc ggatgttttg cggaatcgca gcaaatgccg cagttgcgct ggagaaggcc
     3541 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
     3601 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
     3661 aatatcccgc cccgttagat gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
     3721 tgcaccacga tagtgcaaca aacctagcgt gcaccatgat gatcattcca tcaggtacag
     3781 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
     3841 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
     3901 agcatcatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
     3961 tgatgagcca gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
     4021 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
     4081 atcctggttg gcataggcca ccacatccac gcctttgcta cgccaggtct cgttagcgta
     4141 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
     4201 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
     4261 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatggcgtc
     4321 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
     4381 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
     4441 acctttagca tctttcgacg agaacggcgc ataggtggta tcggttccga tacgtaccgt
     4501 ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaaca aagacagagc
     4561 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
     4621 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
     4681 cattcaacgt taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca
     4741 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
     4801 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
     4861 gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct
     4921 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaaca ggaggcataa
     4981 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
     5041 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
     5101 tcagtaagcc atcagtatag ctatggacaa tgccggaaag ggttttgatt gaacagggta
     5161 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
     5221 catcgtgcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
     5281 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
     5341 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
     5401 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
     5461 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
     5521 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
     5581 aacgtatcga ggaaatcgag gtagtcctga tcaacatctt tggtgacgta gacgccgttg
     5641 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
     5701 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
     5761 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
     5821 aagcgaattt ccggggccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
     5881 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
     5941 tttttatcgc ggaactcggc gcggttggcg ttcagtttac ggcgcacgga cttacgacgc
     6001 agctgctggc ccggcatgat gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
     6061 cggtacggct tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttccggg
     6121 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcacccagt
     6181 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
     6241 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
     6301 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
     6361 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
     6421 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
     6481 aaagcaacca taccgtggcc gataatcatc gccacacagg catatgcgcc gcggattaag
     6541 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
     6601 ttgtccagct cgctagcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
     6661 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
     6721 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
     6781 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
     6841 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
     6901 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
     6961 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
     7021 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
     7081 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ctgcgggatc
     7141 agctgtgatt tgctccagtc ttcgcttttc gacaccccgg taaaggagtc gagaaagaag
     7201 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
     7261 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
     7321 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa ttccatttcg aaccagttcg
     7381 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
     7441 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc
     7501 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
     7561 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
     7621 gtagggattt ccttcccacg catcaataaa aatggcgctg aaaaaatatt caacgccatc
     7681 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
     7741 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
     7801 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
     7861 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
     7921 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
     7981 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
     8041 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
     8101 gcaggtggtg cttcaacctt cggtttaggc ggctccaccg gtttcggctt cggtggggcg
     8161 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
     8221 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
     8281 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cgcgatcgcc cgcttttgga
     8341 accagcggga tagccgcaaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
     8401 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
     8461 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
     8521 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
     8581 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
     8641 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
     8701 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
     8761 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
     8821 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
     8881 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
     8941 cgccagcgct gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
     9001 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
     9061 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
     9121 cggctcaccg acaattgccg gttttgcgct gcgaaagatg cctgctttct cgcgaccaat
     9181 actttcgcga tctggcccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
     9241 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
     9301 caccacgtca agttgtgcct gtttgaacag ccacaacgcc gacagcgtac cgtactcgaa
     9361 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
     9421 cgattccggc aattcctgac cctgcacacg tacgcgctcg gtataacgca ccagatgcgg
     9481 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
     9541 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcagaac
     9601 gccaagacga gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
     9661 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
     9721 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
     9781 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
     9841 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
     9901 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
     9961 caggaattca ctgcgctgga atcctagcgg cagtttttcg cgaacggtct gttcgataac
    10021 acgcggaccg gcaaagccaa ttaacgcttt cggttcagcg atgttgagat cgcccagcat
    10081 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    10141 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    10201 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    10261 ttccagcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    10321 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    10381 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagaagcca gacggtcttt
    10441 atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc tacccatctc
    10501 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    10561 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    10621 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    10681 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    10741 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    10801 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    10861 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacgaataaa ttcgaaaacc
    10921 ccaggcaata ttgaaacaaa aataatgcca acaatcatca gcttaaggtt gctctgaatg
    10981 aaggggagca tgccgaagaa ataacccgcg taggtaaaaa tcagtaccca caacagtgcg
    11041 ccgatcacgt tataagcggc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    11101 ggcgcgaacg ttctgacgat cggtacaaaa cgggcgagaa taatcgtttt gccgccatgt
    11161 ctctcgtaaa actgatgagt cttatcaaga taactgcaac gaaagatttt cgaatccggg
    11221 ttactgaaca gtttgtcacc aaataaccgt ccaatggtat agttcactgc atcaccgaca
    11281 atagcggcaa tcaacataag caccaccatc atatggacat tgagatcgtt agtttccagc
    11341 gacgccaatg caccagcaac aaacagcagc gaatcaccag gcaggaatgg cgttaccacc
    11401 agccccgttt cgcagaacag aatcaaaaac agaatggcat aaacccagac gccgtactcc
    11461 gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    11521 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    11581 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    11641 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    11701 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    11761 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    11821 atgtacaaag gcattcgctt tgatatctac caccacataa ggaccgtgac gcgtgacatt
    11881 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    11941 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    12001 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    12061 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    12121 tttaacccaa cgcacggcga tgtcatcagg taaattcgca tttacgccca aggtccacgc
    12181 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccacc tgcccggtac cgtgtacccc
    12241 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    12301 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    12361 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    12421 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    12481 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    12541 ttacgcacgc agccaacaga aagatgcgga gtacccgaag catcccctac ctgagtcggg
    12601 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    12661 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    12721 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    12781 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    12841 atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    12901 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    12961 atcaggctgg taacactgat acgcgacaaa ccgtcctgat cgattaacgg tttcagtgcc
    13021 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    13081 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    13141 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    13201 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    13261 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    13321 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    13381 gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    13441 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    13501 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    13561 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    13621 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    13681 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc gggccatgca
    13741 gcgtaatgcg accaaactct ggcgcgggca gtaatgtatc cagcgcaacg tgctgttcat
    13801 gcccaataaa cttgctataa gcttcaaaca cttgcgtagt accgcgtgct ttaccttcca
    13861 gggtataacc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaac tccacgttaa
    13921 gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct tcattcaggc
    13981 aggtcagcaa cgcagtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    14041 ccggcttcag gctacggatc agtttttcat ccgccagatg cagcgttttg tacggcccat
    14101 ctttgaagag tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat tcatccagcg
    14161 agcggaaatc accctcatcc ccacggtcag cgcgaggcgg atcgcaaagc aatgttttaa
    14221 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    14281 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagt agtgaggaga
    14341 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    14401 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtg ccaacaaatt
    14461 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    14521 catccgcgtc tgccagttga gcgacggcga ttggacgccc gggaaccgcg gtcacctcac
    14581 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    14641 ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag
    14701 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    14761 cctcctgggc aaccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    14821 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    14881 caacagcaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    14941 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    15001 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    15061 ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    15121 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    15181 atggttattc cgcaaccgca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    15241 cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa
    15301 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    15361 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    15421 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    15481 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    15541 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    15601 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    15661 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    15721 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    15781 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    15841 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    15901 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    15961 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    16021 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    16081 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    16141 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    16201 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    16261 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    16321 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    16381 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    16441 gccattccgt tccatgacat cactttgcca gagtgttttg cccctacgat gcctaatccc
    16501 caggtcagag cgcctgtcag taactggctt tcaccaaagc caagaatcaa acgcccgacg
    16561 accaacaggg caaatttcaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    16621 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc accatattga
    16681 tcggccagtc gcccggcata gccacgcgtc agcaccgtag ccagaaactg aatcccgacg
    16741 gcaatcccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    16801 ggcaacggca gccctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc
    16861 gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    16921 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacagtgcg
    16981 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    17041 tggaactggc ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    17101 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    17161 cctactccga ctactccgac ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt
    17221 ggcgttggtg ccgccgaagc cgaagctgtt agacataacg gtggtcagtt cgcgatcggt
    17281 cgtttcggtc acgatgttca gacctgccgc ctgctcgtcc agctcttcaa tgttgatgct
    17341 cggggcgata aagccgtgtt ccagcatcag cagagagtag atagcttcct gtacgccagc
    17401 agcgcccaga gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc
    17461 gaacacttca cggatagctg ccagttcttt cacgtcgcca accggagtcg aagtaccatg
    17521 agagttcagg taatcgattg gggtatcaac gccgtgcatc gccatcttca tgcagcgtac
    17581 tgcgccttcg ccagacggag caaccatgtc tgcaccatca gaggttgcgc cgtagccaac
    17641 gatttcagca tagatgtgag caccacgcgc cagcgcgtgt tccagctctt caaccactac
    17701 cataccgccg ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc
    17761 tttttccggg gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga attcgcaggc
    17821 catttcccag cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg
    17881 gatctgctct actgcgttac cgatacagtg tgcggaagtc gcacacgcgg agctgatgga
    17941 gtagttaacg ccatgaattt taaacggcgt ggcgaggcag gcagaaacgc cggatgccat
    18001 cgctttggtg accacatacg ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc
    18061 agcgccgaat acctggaaac gcggggaacc gccgccagaa cctgcaatca ggccaacgcg
    18121 cgggttattc tggtaagctt ccggagaaag gcccgcatct gcgattgcct gctccataga
    18181 aaggaatgca taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc
    18241 agtggtatcc agttttacgt tgccccagac gtggctacgc atgccggaat ccttaagctc
    18301 ctgagagaaa gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt
    18361 attaccgatg ctggaaacaa tgcccaggcc agtaatcact gcacgtttca ttcaatacct
    18421 ctgtaagtcg cacatagagt aagtttcgaa tgcacaatag cgtacacttg tacgccgaac
    18481 aagtccgatc agccatttaa tagagaaatt tgcgcagcct tacacacatc gctaagatcg
    18541 agccaccgcc tgtaagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc
    18601 tcgaatttaa tgctgagggt acacctgttt cccgagattt tgacgatgtc tatttttcca
    18661 acgataacgg gctggaagag acgcgttatg tttttctggg aggcaaccaa ttagaggtac
    18721 gctttcctga gtatccacat cctctgtttg tggtagcaga gagcggcttc ggcaccggat
    18781 taaacttcct gacgctatgg caggcatttg atcagtttcg cgaagcgcat ccgcaagcgc
    18841 aattacaacg cttacatttc attagttttg agaaatttcc cctcacccgt gcggatttag
    18901 ccttagcgca tcaacactgg ccggaactgg ctccgtgggc agaacaactt caggcgcagt
    18961 ggccaatgcc cttgcccggt tgccatcgtt tattgctcga tgaaggccgc gtgacgctgg
    19021 atttatggtt tggcgatatt aacgaactga ccagccaaca ggacgattcg ctaaatcaaa
    19081 aagtagatgc ctggtttctg gacggctttg cgccagcgaa aaacccggat atgtggacgc
    19141 aaaatctgtt taacgccatg gcaaggttgg cgcgtccggg cggcacgctg gcgacattta
    19201 cgtctgccgg ttttgtccgc cgcggtttgc aggacgccgg attcacgatg caaaaacgta
    19261 agggctttgg gcgcaaacgg gaaatgcttt gcggggtgat ggaacagaca ttaccgctcc
    19321 cctgctccgc gccgtggttt aaccgcacgg gcagcagcaa acgggaagcg gcgattatcg
    19381 gcggtggtat tgcctgcgcg ttgttgtcgc tggcgctatt acggcgcggc tggcaggtaa
    19441 cgctttattg cgcggatgag gcccccgcac tgggtgcttc cggcaatcgc cagggggcgc
    19501 tgtatccgtt attaagcaaa cacgatgagg cgctaaaccg ctttttctct aatgcgttta
    19561 cttttgctcg tcggttttac gaccaattac ccgttaaatt tgatcatgac tggtgcggcg
    19621 tcacgcagtt aggctgggat gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg
    19681 atttacccgc agaactggct gtagccgttg aggcaaatgc ggttgaacaa attacgggcg
    19741 ttgcgacaaa ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc ccagcagaac
    19801 tgacccgtaa tgtgctggaa ctggcgcaac agcagggttt gcagatttat tatcaatatc
    19861 agttacagaa tttatcccgt aaggatgact gttggttgtt gaattttgca ggagatcagc
    19921 aagcaacaca cagcgtagtg gtactggcga acgggcatca aatcagccga ttcagccaaa
    19981 cgtcgactct cccggtgtat tcggttgccg ggcaggtcag ccatattccg acaacgccgg
    20041 aattggcaga gctgaagcag gtgctgtgct atgacggtta tctcacgcca caaaatccgg
    20101 cgaatcaaca tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca
    20161 gtgaggacga tcagcagcag aatcgccagc ggttgattga ttgtttcccg caggcacagt
    20221 gggcaaaaga ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc
    20281 gcgatcatct gccaatggta ggcaatgttc ccgattatga ggcaacactc gtggaatatg
    20341 cgtcgttggc ggagcagaaa gatgaggcgg taagcgcgcc ggtttttgac gatctcttta
    20401 tgtttgcggc tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg
    20461 cggcgcagat gagcgacgaa ccgattccga tggatgccag tacgctggcg gcgttaaacc
    20521 cgaatcggtt atgggtgcgg aaattgttga agggtaaagc ggttaaggcg gggtaatctg
    20581 ctctcgcagt gtttgccgga tgcggcgtga acgccttatc cgacctacgt gtgacgtgta
    20641 ggcctgataa gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac
    20701 tgctgtgacg cctgctgaaa caggttttcc cacatatcgg tcaccagcgc ctggtcacgc
    20761 ggcgacaatt caccggcacc aatggctttt tccagactct ggctaacggt cgtatgcacc
    20821 gcctgagcgg agtggtcatc accactttcc agttctgcga tggctaatgt caggtggcca
    20881 cgcaaatacc cactggcaaa caactcatca tcactggcat ggtcaatcat gccgtcgatt
    20941 aatgccagaa tgcgtgattc aaactccgcg atcatcttct ttcctcattg taaaacgtgg
    21001 caccggcttc agttgagcgc ttccggccac gggaactgtt ccgccgtaag ttccggcgtg
    21061 tgataataat tttgtagtgc tttaatgaag cgtgccggac gctcgggaat gccatttgtc
    21121 agataatcca tcacctgcgc atgtacccgc cgttgaaaca ccacacgatc cggttcgaag
    21181 tcgccttcca gattgtcgca actgacgtta aacggatatc ccgccgccac acagaacaac
    21241 cagtcgagtg cctgcggctt cacttcaaca tcttcaaact ggctttgcgt ttgggcatca
    21301 cgcccatccg ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc ttttccggca
    21361 atacaccagt gcgaaatctc atgaattgcg ctggcataaa agccgtgggc aaagacaatt
    21421 cggttatacg gtacttccgc atcagcagga agatagatcg gttcgtcgtc gcctttaatc
    21481 agacgggtat taaattcatc ggcaaagcag ctattaaaaa tttcaatcaa ctgctcatag
    21541 tggtgtgtac tgttcattag ttcatcccca accagtggag gatctcctgt ccgtggctat
    21601 cgtaaagtaa tttggcactc atcaccgccg agacgataac gatcatcggg cggatcagtt
    21661 tttgtccttt gctcagcacc agtcttgaac ccatgcgcgc gccgaggaac tgccccacca
    21721 gcatgacaaa gcccgtcgcc caaatcactt tgccgccgag aataaacagc agcagaccgc
    21781 caatgtttga cgttgcgttg agtaatttgg cgtgagccgt ggctttggcg aggttgaatc
    21841 cgcacagcgt aacgaaggcc agcgcgtaaa acgatccggc agccgggcca aagaatccgt
    21901 cataaaaacc gacgcagcca ccggcaatta acgcgaaggg taaaccgtac atccggcgct
    21961 ggcgatcttc ttcacccagc tttggcatca gcaagaaata gaggccgata caaattacca
    22021 gaatgggcaa aatctgccgt aagacatcag cctgaacgta ttgcaccagc agtgcgccgc
    22081 tcattgagcc ggcaaaggtc atggcgatat tgagtttctg atcgcttaaa ctaaccactt
    22141 tgcggcgaat aaagtagata gtggcggaaa tagagccgcc gcaggcttgc agtttattgg
    22201 ttgccagcgc gttagcggga gacattcccg cagccatcaa tgccggaatg gtgagtaacc
    22261 caccgccacc ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa aagagaactc
    22321 ccagcaacag cggggaaacc ataaacaggc tattaaacgt ttccattaga tcacgtgctc
    22381 atccagtagc gcctggcagg aaggcggcaa cggaggcggt gtcttcttct caggctttgt
    22441 tgttcctggt tttggaggtg caaaccagct ttgcagttct gccccgcaac catcgcctgg
    22501 tggcggtaaa ggttgatctt cacactccag actatcggca gggcaacgta atcgtacatg
    22561 catatgcgcg cgatgctgga accagggtcg cactttgcgc aaccagtcgc gatcggtgcc
    22621 cgcatcaagg caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt
    22681 gtcctgggcg gcgagtttga tcaagctgaa aatttctggc ttccacagcg tggagacaac
    22741 gtgtttaccg tcgcgggaaa ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt
    22801 ccagcgagtt ttcggcagtt gcagaaagat atcgacatcc agtccggtct ggtggctggc
    22861 atgaccgccg ttgaaacgcc caccagcggg catccccata tcgccaatca gcaccgtacc
    22921 catgcccaga ttgctcacct ggctactcag acgctggata aacatcacca gatccgggtg
    22981 accgaaatag cgacgctgat cggtacgcat gacctgataa tgttcggact gtatcggcaa
    23041 cgtgtcagcg ccgacaatac agccattaga aaaactgcct atcgattgtg cgctacccgg
    23101 cacaggttgg gttatttttt gccacggcgt cgctgccaag ctagcgctac tggcaagcag
    23161 agccagcagc gcaatcgcgg ttttgttcat tttttaccag cgtggaatat cagtcttcac
    23221 atcggcattt tgcgcccgct gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc
    23281 ttcagcgatc ggcactgcac ggatcccgac acagggatcg tgacgacctt tggtgatcat
    23341 ctcaacttct tcgccaaagc ggttaatagt gcgccccggc acggtaatgc tggaggtcgg
    23401 tttcagcgcc atatgagcaa tgatttgctg cccgctgctg ataccgccga gaatgccgcc
    23461 cgcatggttg ctctggaaac cgtctttggt gatttcgtcg cggttctggc tgccgcgcag
    23521 cgccactacg tcaaagccgt cgccaatttc cacgcctttc accgcgttga tgctcatcag
    23581 cgcatgggcg atgtcggcat ccaggcggtc aaataccggc tcgccaagtc cggcggggac
    23641 gccactggca acaacggtga ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg
    23701 catcagttca tctaacgcgt cgattttgtc cggatccggg cagaagaacg gattttgttc
    23761 aaccagcgac cagtctttga tttccagcgg aatgtcgccc atctgggtca agcagccgcg
    23821 gatttcaata ccaaatttct cggcgagata ttttttggca atcgcccctg ccgccacgcg
    23881 catggcggtt tcgcgggcgg aagaacgtcc accgccgcga taatcgcgca gaccgtattt
    23941 ttgttcgtag gtgtaatcag catggcctgg gcggaaaacg tctttaattg cgctgtaatc
    24001 ctgagagcgc tggtcagtgt tttcgatcaa caagccaatg ctggtgccag tagtaacgcc
    24061 ttcaaaaaca ccagagagaa ttttgacctg atccggctcg cggcgctggg tggtatagcg
    24121 cgatgtccca gggcgacgac ggtcgaggtc atgttgcagg tccgcttccg tcagcagaat
    24181 gcctggcgga acaccatcga cgatgcagcc gagcgccagc ccatgcgatt cgccgaaggt
    24241 ggttacgcga aagagttgtc caattgtgtt tccagccatc acggctccgt tattgttgtg
    24301 tttgcgtgtt tacttaatct ttataaatcg cgaaatattc acgggccgcc agcagctgct
    24361 ctttggtgag cataaacaca ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa
    24421 catccggata ttgttccata agatgtacca tgctgttgcc gacttcacaa atcaacacgc
    24481 catcatcagc aaggtaatct gccgcgtttc caagaatgcg acgcgtcagt ttaaggccgt
    24541 cggtgccaga tgccaggccc agttccggct cgtgacggta ttcgtttggc aggtcggaca
    24601 tatcttccgc gtcgacatac ggcgggttag tgacaatcag atcgtactgc actttcggca
    24661 agtcgcggaa cagatcggaa cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga
    24721 tgttctgttc agcaaccgcc agcgcgtcgg gagagatatc caccgcgtcg acttctgcat
    24781 ccgggaaggc ataagcacag gcaatggcga tgcagccgct accggtacac atatctaaaa
    24841 tatgctgcgg ttgtttgctg atgagtccgg caaatttatt gttgatcagt tcaccaatcg
    24901 gcgagcgcgg caccagcacg cgttcatcga cgtaaaattc atgaccgcag aaccaggctt
    24961 tgttggtcag gtaagccacc ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa
    25021 tacgatgttt ttcgctggag gtcagacgcg cggtgcgcat atcttccggg atatccagcg
    25081 gcaggtagag cgatggtaac accagctgta cggcttcatc ccacgggtta tcggtgccgt
    25141 gaccgtacca gatatttgcc gcgctgaagc ggctcactga ccagcgcaac atgtcctgaa
    25201 tggtttgcag ctcatttact gcttcatcaa cgaaaatttt atccacgtat tcctccaggg
    25261 catgatcgca ataatttcgg cggctagttt gccatgaaga tgacgataaa tcagcattca
    25321 cgcgcggtga gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa
    25381 ctgtaggaaa attagaaata gagacagata aatgaaaaag aaaacaacac ttagcgagga
    25441 ggaccaggct ctgtttcgcc agttgatggc ggggactcgc aagattaagc aggacacgat
    25501 tgtccaccga ccgcagcgta aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga
    25561 gcaggctgat gccagccatt atttctccga tgagtttcag ccgttattaa ataccgaagg
    25621 tccggtgaaa tatgttcgcc cggatgtcag ccatttcgag gcgaagaaac tgcgccgcgg
    25681 cgattattca ccggaattgt ttttggattt acacggtctg acgcagctgc aggccaagca
    25741 ggaactgggc gcgttgattg ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat
    25801 gcatgggcac gggaagcata ttttgaagca acaaacaccg ctgtggctgg cacaacatcc
    25861 gcatgtgatg gcatttcacc aggcaccgaa agagtacggc ggtgatgcgg ctttgttggt
    25921 gttgattgaa gtggaagagt ggttgccgcc ggagttgccc tgaggagttg agcgtgttgt
    25981 gccaggagcg cggaatggca ggggcgaatg accctgccag gtgagaaaag gtgcgctaat
    26041 acatattcag tatgtaagtg aatcggcagg tagaatctat gggctgtcga gatgtcgtgt
    26101 ccacgcgtag cttcatatca tgcagttgcc tggacgtaat cggttgcggc tcaggcgtca
    26161 ccgtcacttt ggcattatca cagccggaag tcgagaaggt catctgctgt gcgataaaac
    26221 ggcagtagac accacgctca tcaaaattat tcccgttaca cagtttgcca ttttccatca
    26281 ggaaagtggg cagtgtttgc tcaataacac cacctgttga ctgcaacctg acgactttgt
    26341 tatgtacaac ataattttca ttccaggaaa catcatacgg tgccaggggc accgccggac
    26401 atgaagaact caccttcgtt gggccaatat agtcaggaaa atcatcatca tacgttttag
    26461 cgaagggatc tctactctta acaaacgacg acacaatcca cggacaacca tgtaaattcc
    26521 catcgtaata aacaaacatc tttaattcat tacatgtcca tcccgttttg gtctggctat
    26581 catactgctc taatccagga caggctgaca tggataccct gctgtccgaa attttcgaca
    26641 tagatggttg agtagaacta tcgccagtcc acgaaactaa atccggctgt gaattgggca
    26701 tgaatgtagc cttaaatata gcgtaattaa ctgaatctgg cgtcgctata atcagatgaa
    26761 actcaaa
//
LOCUS       DADLTD010000033        26565 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLTD010000033 DADLTD010000000
VERSION     DADLTD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.033"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1985
                     /locus_tag="JN701_001122"
     CDS             <1..1985
                     /locus_tag="JN701_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HAY7363639.1"
                     /translation="VGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHN
                     TPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDI
                     NDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILT
                     NQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTR
                     NGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCP
                     AGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQS
                     GSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQY
                     GKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLW
                     SQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLN
                     VYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            1991..2704
                     /gene="ybiX"
                     /locus_tag="JN701_001123"
     CDS             1991..2704
                     /gene="ybiX"
                     /locus_tag="JN701_001123"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY7363640.1"
                     /translation="MWGAGPTFWRNCMMYHIPGVLSPQDVARFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="JN701_001124"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="JN701_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY7363641.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3309..3569
                     /locus_tag="JN701_001125"
     CDS             3309..3569
                     /locus_tag="JN701_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7363642.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3797..4870)
                     /locus_tag="JN701_001126"
     CDS             complement(3797..4870)
                     /locus_tag="JN701_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY7363643.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPE
                     LFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGSWQAI
                     CDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5011..5973)
                     /gene="ybiB"
                     /locus_tag="JN701_001127"
     CDS             complement(5011..5973)
                     /gene="ybiB"
                     /locus_tag="JN701_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY7363644.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6001..8151)
                     /gene="dinG"
                     /locus_tag="JN701_001128"
     CDS             complement(6001..8151)
                     /gene="dinG"
                     /locus_tag="JN701_001128"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY7363645.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEHEGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8271..8753
                     /gene="ybiA"
                     /locus_tag="JN701_001129"
     CDS             8271..8753
                     /gene="ybiA"
                     /locus_tag="JN701_001129"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY7363646.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8985..10349)
                     /gene="rhlE"
                     /locus_tag="JN701_001130"
     CDS             complement(8985..10349)
                     /gene="rhlE"
                     /locus_tag="JN701_001130"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAY7363647.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10578..11249
                     /gene="cecR"
                     /locus_tag="JN701_001131"
     CDS             10578..11249
                     /gene="cecR"
                     /locus_tag="JN701_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY7363648.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11249..12247
                     /gene="hlyD"
                     /locus_tag="JN701_001132"
     CDS             11249..12247
                     /gene="hlyD"
                     /locus_tag="JN701_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY7363649.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12240..13976
                     /locus_tag="JN701_001133"
     CDS             12240..13976
                     /locus_tag="JN701_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7363650.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13969..15102
                     /locus_tag="JN701_001134"
     CDS             13969..15102
                     /locus_tag="JN701_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7363651.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15113..16219
                     /locus_tag="JN701_001135"
     CDS             15113..16219
                     /locus_tag="JN701_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7363652.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITRQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16181..16591)
                     /locus_tag="JN701_001136"
     CDS             complement(16181..16591)
                     /locus_tag="JN701_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363653.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16724..17475
                     /locus_tag="JN701_001137"
                     /pseudo
     CDS             16724..17475
                     /locus_tag="JN701_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17472..18713
                     /gene="clsB"
                     /locus_tag="JN701_001138"
     CDS             17472..18713
                     /gene="clsB"
                     /locus_tag="JN701_001138"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY7363654.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGETQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18779..19669
                     /locus_tag="JN701_001139"
     CDS             18779..19669
                     /locus_tag="JN701_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY7363655.1"
                     /translation="MIVLLVVYAKKVDWEEVWKVIRDYNRVALLSAVGLVVVSYLIYG
                     CYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTWVGGIGMRYRLYSRLGLPGSTIT
                     RIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVAQTTLRILGIGLLMIIAVYLWFC
                     AFAKHRHMTIKGQKLVLPSWKFALTQMLISSVNWMVMGAIIWLLLGQSVNYFFVLGVL
                     LVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGSIIAALLAYRVLYYFIPLLLALI
                     CYLLLESQAKKLRAKNEAAM"
     gene            complement(19705..20418)
                     /locus_tag="JN701_001140"
     CDS             complement(19705..20418)
                     /locus_tag="JN701_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7363656.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20624..21328)
                     /locus_tag="JN701_001141"
     CDS             complement(20624..21328)
                     /locus_tag="JN701_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7363657.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21465..21917)
                     /gene="moaE"
                     /locus_tag="JN701_001142"
     CDS             complement(21465..21917)
                     /gene="moaE"
                     /locus_tag="JN701_001142"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY7363658.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21919..22164)
                     /gene="moaD"
                     /locus_tag="JN701_001143"
     CDS             complement(21919..22164)
                     /gene="moaD"
                     /locus_tag="JN701_001143"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY7363659.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(22157..22642)
                     /gene="moaC"
                     /locus_tag="JN701_001144"
     CDS             complement(22157..22642)
                     /gene="moaC"
                     /locus_tag="JN701_001144"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY7363660.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22645..23157)
                     /gene="moaB"
                     /locus_tag="JN701_001145"
     CDS             complement(22645..23157)
                     /gene="moaB"
                     /locus_tag="JN701_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY7363661.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(23179..24168)
                     /gene="moaA"
                     /locus_tag="JN701_001146"
     CDS             complement(23179..24168)
                     /gene="moaA"
                     /locus_tag="JN701_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY7363662.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVCCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(24156..24300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24565..25473
                     /gene="yvcK"
                     /locus_tag="JN701_001147"
     CDS             24565..25473
                     /gene="yvcK"
                     /locus_tag="JN701_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY7363663.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25665..>26565)
                     /locus_tag="JN701_001148"
     CDS             complement(25665..>26565)
                     /locus_tag="JN701_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363664.1"
                     /translation="RPLKFEEFEALAPQTIYVSATPGNYELEKSGGDVVDQVVRPTGL
                     LDPIIEVRPVATQVDDLLSEIRQRAAINERVLVTTLTKRMAEDLTEYLEEHGERVRYL
                     HSDIDTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERS
                     LIQTIGRAARNVNGKAILYGDKITPSMAKAIGETERRREKQQKYNEEHGITPQGLNKK
                     VVDILALGQNIAKTKAKGRGKSRPIVEPDNVPMDMSPKALQQKIHELEGLMMQHAQNL
                     EFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         6297 a         7143 c         6893 g         6232 t
ORIGIN      
        1 gcgtgggtgc gtttttggcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg cattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggca cagcgcaccg acgggctcga tcaatatgat cagcaaacag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gataccaccg cggtgcgcct gaatgtaatg ggcgaaaaaa
      361 cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc acccagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gaacggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga
      781 cctggtcacg cacggcgaat accaaagatg taagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accggttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga aacgcagact aactacggcg ttaatccggt gacgttaccc gcggtaaata
      961 tttatcatcc tgacagcagc attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg cctttgatac gctgcaaatc acccgtgatt
     1081 ttgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccagc tggtgtggca aaaggttctc
     1201 cggtcaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa gccggggcgc
     1261 tgtatcacct gacggaaaac ggtaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagcgg taacagtgcc aaccgcacag
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgcctgtt gctcaccgcc gcactgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg aacttactcg caatacggta agaaacgtgt cgaaggctat gagatatccg
     1561 tggccgggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag caaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccagtatc aggcaaccga tgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atgcataaag gttcagacgg cgcggtggga acgccagcgt
     1801 ttaccgaagg ttactgggtc gccgatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtttac aacctgtttg ataccgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcaagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga aattgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc tcgttttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg caagttaaga acaatcaaca
     2161 ggtagacacc cgcagcacgt tatacgccgc cctgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgct ttattctttg ccgcggcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt tcatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga caccttcgga caacatcggg taaaactccc
     2461 ggcaggtgat ctcgtgttgt atccctccag cagcctgcat tgcgtgacac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactag acaaaaatat tcagtcgctg aaaagccgct atggtgaaaa
     2641 tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgacgcttg aactgcccag gttcgtccct atattagcta cacttaactg tacaagcatt
     2761 gatatgggga ggacgatatg gcatccggtt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgtggcgaaa
     2881 gcctgtatga atgtgaagag tgcggtgccc ccatcccgca agcccgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcgtata
     3001 caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataatttgt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggc ataaaacaag tccataagta caacaaataa atggtttatc agtatgttag
     3181 atgtcaatca atttatttga acaaggcggt caattctctt cgattttatc tctcgaaaaa
     3241 aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc acctgcgaga
     3301 gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca actctatcat
     3361 ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg aataaaatcg
     3421 gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcgaaa ctgactgaga
     3481 aagccgcggc agccggtgcc agtggataca gcatcacttc tgccaccaac aacaataaat
     3541 taagcggtac tgcggtaatt tataagtaat tcgtaggacg gataaggcgt ttacgccgca
     3601 tccggcaaca gatgcctgat gcgacgctac cgcgtcttat caggcctaca aaatccgaac
     3661 cgttggacgg atcaggcggt tacgctacgt ccagcacaag tatttccctg tctgtttgcc
     3721 gacagacgca tatgctctaa ccctcattga tcctatatta cccttgtttg cccgtccgtt
     3781 actggacggg ctttttttag ctggctaact gctgacagaa agcctgcaac gtctcttccg
     3841 acataccaat ctgccgcgcc gcatcacaaa tggcctgcca gcttcccgca tccaatggaa
     3901 tcccctgctc ctgccgttcg cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg
     3961 gcttatcgtc atcatgcggc gaggctttca cccactcggc aaaggcttca gtctgcgcgc
     4021 tacaatccgg cgcgccgaac agttccgggt tgatgatgat agtggtcatg cagttaagaa
     4081 tggcatcggg actggtttgt aacgtttcct gatgcgtcgt tttaccgcca gaaagcgccc
     4141 cgccaagaat ttcacacatc gctgcaaggg cgtagccttt atgttcggca aaggtcaaca
     4201 gcgacccctg cattaccgcc ggattggtcg tcggcacgcc gttaacgtca atcaggcaac
     4261 ctggcggcac ggggacgcct ttatgccagg cgacgcgagt tttaccaaat gcgatggcgc
     4321 tggtggcgta atcaagcaac agcgggaaat tatctttacg agggaaaacc acacagaacg
     4381 gattggtgcc aaagcggctg tcgcgaccgt ggaacggtgc gaccattgga ataccgacca
     4441 cgctaacaaa gtggatagag acaaaccccg ccgctgcaca ctgctccgcc cagtaaccga
     4501 tacggccgat atgatgcgag ttatgtagcg ccacggcagc aataccgtgc tgatgcgctt
     4561 tctcaatccc cagcgccatc gcttcgtgtg ccgcgacctg accgaatgcg cgatcgccat
     4621 cgagcgtgac tgccgccccc gcctctttaa cgattttggc atgatggtta atttgcagat
     4681 gcccctgact ccaggaacgc acatagcttg ggatcatgcc aataccatga gaatcatgcc
     4741 ctgccaggtt tgccgcgatt aaatgatcgg ccactaattt cgcttcttgt tcctcgctac
     4801 ccatctgacg aaatacagcc tgaataaaac tgtgcagcgt ctgagcatca aagcgatgac
     4861 cactttccat ggttaactcc tgtttatgtt atgtgtttgt tgttttttta tgctgcacgc
     4921 cgggcgcggg aaggtcaata aagatgcagg aatttcatat tgcggtgggg taaaagccgg
     4981 atgacatggc tcatccggct gagaaaagaa ttaaaatgcc tgattaacgc gcgccaggcc
     5041 gtcgctgata gttgccgctt cacccgtagc ctccaggcag caagccatct ggattttcag
     5101 cgattcggga atcggttcgc tgccagcaag gcaacgctca atccactgcg ccgtggtttc
     5161 tgggtctttt gcttgtggca gtaactcgct accagcagtg tcctgttttt catacagcac
     5221 ccgcatccct tcacggtcaa tgagattgat ctgcgggcag cgctgcggat tagcatacac
     5281 ttcgccttca gtaccatgca ttagtagcgc ccgcccgccg atatcgctaa agaacttcgc
     5341 gactcgcccg atgtattccg gatgcgaaac gctggaaaga cgcagcgcct caccttcggc
     5401 aaatggcgtc gccagtttcg ccagggtatg tgcactgtta cgcacgccca tccgccagcg
     5461 catcgccagt tgtttttcca gcggcgggca aaacgcgccg acaggcataa acaccggttg
     5521 atgttcgtcg agcttcgcct gcgcctgccc gccgtgcaac gttggtgtaa tacccatcag
     5581 ttcgaaaatg gtttctgtca gcacgcgagt tggatcttcg ctaaccccgt gaaccaccac
     5641 aggaaaaccg agtttgtgca ggagaatagc caacaacggc gtcaggttgg cctgtttacg
     5701 cgcgccgttg taactgggga tgacaatcgg catcggcttg cctgcaggcg gcgtcagctt
     5761 gatggtgtga ttttgcatgg cttcgtaaaa gccgagcatc tctgcttccc cttccccttt
     5821 gatacgtagc gcaatcaata cgccgcccaa ctcaaggtca gggacttcac cattgagcat
     5881 atgggcatac agaccgcgcg cagtatcccg gtctaaatcg cgcgcgtggt ttttcccgcg
     5941 cccgatctct ttaatgattt tgcgatagtc catttacgac tccttacctg actcacatca
     6001 ttaacgccgc cgacggcgtg gggatttcgt tttttctttc tttttaacta taccttcagg
     6061 gacttccggt tgctctatcg gaaataccgg taatgcatct agtagtcgct tgccataatt
     6121 tttggtcagc aagcgtttgt cgtagataac cacttcgccc cagcaaccgt ggcttcgaat
     6181 cagtcgccca acctgctgaa tcaggttaaa cgaggcgctc ggcaggcttt gcacctcaaa
     6241 cggatagcgg ttgaggcttt tcagccattc cccttcggtg atcaccaccg ggctgtcgat
     6301 gggcggaaaa gcgattttgt ggatatgcac ctggctgagc agatcacctt tcaaatcaag
     6361 cccttcggca aatgactgta agcccaccag cacactacgc tcaccgttgg caacgcgttt
     6421 gcggtgcagt tcaactaagc gataacgcgg ctgatcgccc tgcaccagca gcatcaggcg
     6481 taaatccgtc acatagtcga gaaagcgctg catcgcccgt ccgctggcaa acagtaccaa
     6541 cataccgaga tgttttttgc tctccacctg ctcacggaaa aaggccgcca tttcggcaat
     6601 atgctgctct tcgttgtcga tggaaggctc aaagcgcatc cggggaataa caattttgcc
     6661 ctgttcgcag tggttaaagg gggaatccag cgccacaaaa cggtcgcccg ctttctcttt
     6721 cagcccgctc atctcctgca aacgcgaaaa actgttcagc gaacgcaagg ttgcggaggt
     6781 gacaataatg tgcggaatac tgcgccacag cagcctttcc agctgatcgc tgacacgtat
     6841 tcccacgcag tgaaaccaga gatgcagctg cccttcgtgc tcttcccgcg tcgcccattt
     6901 ggtcaccggc gcaccggaag attgcgccag agacgccagc cgccagagtt tgctttgcgc
     6961 ctcgaacatc cccaacgcgc ggttcatctg caaaatcaac cgatgcagac gcacaatatc
     7021 gtggctgccg gttttctcac ttaaatcgtt aagaaataac tccgccagac cacgcagcat
     7081 ctcggtgagt tttgccagcc gctggcagat ctccagcact tcatcaggca gttcgcccat
     7141 cgcaaaacgg tgctctgcct cctgcccggc aggcatgtag agattgagaa tgttgtttaa
     7201 cgaggcgata agctcataca actcttcaca atgcgcattc aaacgttcag ggatcgccag
     7261 cggtgggatg gtcttcgggc gaaactgctc catgcaggta gcgaccagtt tcgtgaacaa
     7321 gtccagctgt agtcgatacc agggcgcggt gatttcagcg ctcatctcca gcgcatcccg
     7381 ggccacatct ggcaggtgat gaccttcgtc cagcaccagc agcagatttt tcgggtcagg
     7441 cagtacggct tcgctttcca tcgccgccat caccagcgca tggtttgcca ccaccacttc
     7501 cgcttcctga atctcccgac gagcaacaaa aaacgggcat tcacgatagt agtaacagtt
     7561 acggttaagg cagctggctt tgtcggtact caaacgacgc cagagatcat catctatagc
     7621 gatatccgta tgatcgcgca gaccatccca tttataagtg tcgagatcgc ccttcagctt
     7681 cgcacaacgt ttttgctctt cctgattatt tggcgtcagt tcgtcgtcaa gaaacgccag
     7741 cagatcctgt tgcgtgggtt cagtgctggc gagcgccgtc agattacgcg gacaaacgta
     7801 gcgcccacgc ccgaaagcgg cagtgaattt aagatcggga atgatctttt tcagcagcgg
     7861 taaatctttg ctgtaaatct gatcctgcaa cgccacgttg gcggtactca ccaccagcgt
     7921 tttttgctct tcgcgggcaa tggcgatgcc ggggatcaaa taggagagcg ttttcccaac
     7981 gccggtgggg gcttcaatcg ccagatgccg cccttcttct ccggccagcg ttttggcgac
     8041 gtccgcaatc atctgccgct gcggcgcacg gggaataaag tcggggatct gttcctgaag
     8101 cgccttatac caggcggcaa tttgcgcttt aagcgcggcg gttaatgcca tgagaaaacc
     8161 tgaaatactg tataaacagc caatattgtg gcatttttcg gttttcaggg gtaatggttt
     8221 ttctgtttct ggaaagcggc tcgcaataac caccaacata atgcggttta atgcccgttc
     8281 gagcacaaag gatccaacac gtcatgcaag acaccatcat aaatttctac agcaccagcg
     8341 atgactacgg tgatttttct aactttgccg cctggcccat caaggttgac gggaaaacct
     8401 ggcccacctc cgaacactat tttcaggcac aaaaattcct tgatgaaaaa taccgcgaag
     8461 agatccgtcg ggtttcttcc cccatggtcg ccgctcgcat ggggcgtaat cgttccaagc
     8521 cacttcgtaa aaactgggag tcggttaaag aacaggtgat gcgtaaagca cttcgtgcta
     8581 aattcgaaca gcatgcagaa ctgcgtgcgc tcttgctggc aaccgcgccc gcaaaactgg
     8641 ttgagcatac ggaaaacgat gcttactggg gagacggtgg taatggtaag ggcaagaatc
     8701 gactgggcta ccttttaatg gagttgcgcg aacaattggc tatagagaag taacgtctcg
     8761 gtggcttatg ggcggtaaaa cagataaatt ttttcccgtg ttctcaaatt gctgacaagt
     8821 gcttgttgtg aaatgccgga tgcggcgtaa acgccttatc cggcctacaa aagcatgcaa
     8881 attcaatata ttgcagagtt aacgtaggcc tgataagcgt agcgcatcag gcagttttgc
     8941 gtttgttcat cagcctgatg ccgggcatag cccggcataa aagattactg cgcagcggca
     9001 ggtttacgcg gacggcggcg acgttgttgt tcgcctgccg gtttggcatc gccgaggcgg
     9061 cgagacggtt tttctgcagg tttcgcgctt gcagattttg cgccaccttc cccacggcgt
     9121 ggttgctgtt gaccgcgacc accaccttgc ccacgaccgc cgccgccacg ttgctggcga
     9181 ccgttctgga tcggttcggc tttgattgac gggtccggct catagcccgg aatcgcaatg
     9241 cgcgggatct cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca
     9301 cacaccagcg acaacgcttc accggtagca gccgcacgac cggtacgccc gatacggtgg
     9361 acataatctt ccggtacgtt tggcagttca tagttgacga cgtgcggcaa ctcttcgata
     9421 tccagaccgc gcgcagcgat atcggtcgcc accagtacgc gaatgtcact cgatttaaaa
     9481 tcagccagcg cacgggtacg cgcaccttgc gatttattcc cgtggatcgc cgcactacgg
     9541 atgccatctt tattgagctg ctcagccaga tggttagcgc cgtgtttggt acgggtaaac
     9601 accagcacct gctgccagtt ccctttacca atcatgtgcg acagcaattc gcgtttgcgt
     9661 ttcttatcga caaagtgaac gtgctgggtc acctgatcag acgcggtatt gcggcgtgcc
     9721 acttcgattt ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag
     9781 aaggtcgcag agaacagcag gttctggcgt ttcgctggca gttttgttaa cacgcgacgg
     9841 atatcgtgga taaagcccat gtcgagcatg cggtcagctt catcgaggac gaggatttca
     9901 acctgatcca gcttcactgc attttgatgt tccaggtcca gcaaacgtcc cggtgttgcc
     9961 accagaacat caacgccgcc gcgcagcttc atcatttgcg ggttaatgct gacaccgcca
    10021 aacaccacca gcgaacgaat gttcaaatat ttgctgtaat cacggacgtt ttcgccaatc
    10081 tgcgcagcca gttcacgggt cggggtaaga atgagcgcac gtaccggacg acgccctttg
    10141 gcgtgcggct ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgtttta
    10201 ccggtaccgg tctgggcgct agccatcagg tcgcggcctt ccagcaccgc agggatcgcc
    10261 tgctgctgaa taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca
    10321 gggcttaaac ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg
    10381 tgtagttttc agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag
    10441 cggagtgtag cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgttgctaa
    10501 aattcttagc catactggac aactccccta tggagtcata atcttaatca atcatttgat
    10561 taagaatgga ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa
    10621 aacagctgat tgctgccgca ctggcgcagt ttggtgaata tggaatgaac accaccactc
    10681 gcgagatagc cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga
    10741 aagaagattt gtacctcgcc tgcgcccagt ggattgccga ttttattggc gagcagttcc
    10801 gtccgcatgc cgaggaagcc gaacgcttgt tcgcgcaacc acagcctgat cgggctgcca
    10861 tccgtgaact gatccttcgc gcctgcagga acatgattaa gctgctcacc caggatgaca
    10921 ccgtcaacct cagcaagttt atctcccgtg agcagctctc tcccacggca gcctaccacc
    10981 tggtgcacga acaggtgatt agtccgctac acagccacct gacacgcctg attgccgcct
    11041 ggaccggctg cgacgccaat gacacccgca tgatccttca tacccatgcg ttgattggtg
    11101 agattctggc gttccgtctt ggcaaagaaa caattctgtt acgtaccggc tggaccgcgt
    11161 tcgatgaaga aaagaccgaa ctgatcaacc agacggtgac ctgtcatatc gacctgattt
    11221 tgcaaggatt atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggactggc
    11281 ggtagtggta cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga
    11341 taacggcctg aagctgtatg gcaacgtgga tattcgtacg gtgaatctta gtttccgtgt
    11401 tggggggcgc gttgaatcgc tggcggtgga cgaaggtgat gcgatcaaag cgggccaggt
    11461 gctgggcgaa ctggatcaca agccgtatga gattgccctg atgcaggtga aagcgggcgt
    11521 ctcggtggca caggcgcagt atgacctgat gcttgccggg tatcgcgatg aagaaatcgc
    11581 tcaggccgcc gcagcggtga aacaggcgca agccgcctat gactatgcgc agaacttcta
    11641 taaccgccag caaggactgt ggaaaagccg caccatttcg gcaaatgacc tggaaaatgc
    11701 ccgctcctcg cgcgaccagg cacaggcaac gctgaaatca gcacaggata aattgcgtca
    11761 gtaccgttcc ggtaaccgtg aacaggacat cgctcaggcg aaagccagcc tcgaacaggc
    11821 gcaggcgcaa ctggcgcagg cggagctgaa tttacaggac tcaacgttga tagcaccgtc
    11881 tgatggcaca ctgttaacgc gcgcggtgga gccaggcacg gttctcaatg aaggtggcac
    11941 ggtgtttacc gtttcactaa cgcgtccggt gtgggtgcgc gcttatgttg atgaacggaa
    12001 tcttgaccag gcccagccgg ggcgcaaagt gctgctttat accgatggtc gcccggacaa
    12061 gccgtatcac gggcagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt
    12121 cgaaacaccg gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc
    12181 cgatgatgcg ttacgccagg gaatgccggt gacggtgcaa ttcggtaacg aggcaggaca
    12241 tgaatgatgc cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atgggcaagc
    12301 ccgccgtcgc gccgctcgat tgtaccattc acgccggtta tgtgacgggg ctggtggggc
    12361 cggatggtgc aggtaaaacc acactaatgc ggatgctggc gggattactg aaacccgaca
    12421 gcggcaatgc cacgatgatt ggctttgatc caatcaaaaa cgacggcgcg ctgcacgccg
    12481 tgctcggcta tatgccgcag aaatttggtc tgtatgaaga tctcacggtg atggagaacc
    12541 tcaatctgta cgcggatttg cgcagcgtca ccggcgaggc acgtaagcaa acttttgctc
    12601 gcctgctgga gtttacgtct cttgggccgt ttaccggacg cctggcgggc aagctctccg
    12661 gtgggatgaa acaaaaactc ggtctggcct gtaccctggt gggcgaaccg aaagtgttgc
    12721 tgctcgatga acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg
    12781 tgcatgagct ggcaagcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag
    12841 ccgagcagtg ccgtgacgtg ttactgatga atgaaggcga gttgctgtat cagggggaac
    12901 caaaagccct gacacaaacc atggccggac gcagctttct gatgaccagt ccacacgagg
    12961 gcaaccgcaa actgttgcaa cgcgccttga aactgccaca ggtcagcgac ggcatgattc
    13021 aggggaaatc ggtacgtctg atcctcaaaa aagaggccac accagacgat attcgccatg
    13081 ccgacgggat gccggaaatc aacatcaacg aaactacgcc gcgttttgaa gatgcattta
    13141 ttgatttgct gggtggtgcc ggaacctcag aatcgccgct gggtgcaata ttgcatacgg
    13201 tagaaggcac acccggcgag acggtgatcg aagcgaaaga actgaccaag aaatttggtg
    13261 attttgccgc caccgatcac gtcaactttg ccgttaaacg tggggagatt tttggtttgc
    13321 tggggccaaa cggcgcgggt aaatcgacca cctttaagat gatgtgcggt ttgctggtgc
    13381 cgacttccgg ccaggcgctg gtgctgggga tggatctgaa agagagttcc ggtaaagcgc
    13441 gccagcatct cggctatatg gcgcaaaaat tttcgctcta cggcaacctg acggtcgaac
    13501 agaatttacg ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa
    13561 tctcccgtat gagcgaggca tttggcctga aaagtatcgc ctcccacgcg accgatgaac
    13621 tgccattagg ttttaaacag cggctggcgc tggcctgttc gctgatgcat gaaccggaca
    13681 ttctgtttct cgacgaacca acgtccggcg ttgatcccct cacccgccgc gaattttggc
    13741 tgcatatcaa cagcatggta gagaaaggcg tcacggtgat ggtcaccact cactttatgg
    13801 atgaagcaga atattgcgac cgcatcggcc tggtgtaccg tgggaaatta attgccagcg
    13861 gcacgccgga tgatttgaaa gcgcagtcgg ccaacgacga gcaacccgat cccaccatgg
    13921 agcaagcctt tattcagttg atccacgact gggataagga gcatagcaat gagtaacccg
    13981 atcctgtcct ggcgtcgcgt acgggcgctg tgcgttaaag agacgcggca gatcgttcgc
    14041 gatccgagta gctggctgat tgcggtagtg atcccgctgc tactgctgtt tatttttggt
    14101 tacggcatta acctcgactc cagcaagctg cgggtcggga ttttactgga acagcgtagc
    14161 gaagcggcgc tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc
    14221 agcgataacc gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg
    14281 gttattccgg tggattttgc ggaacagatg gagcgcgcca acgccaccgc accgattcag
    14341 gtgatcaccg acggcagtga gccgaatacc gctaactttg tacaggggta tgtcgaaggg
    14401 atctggcaga tctggcaaat gcagcgagcg gaggacaacg ggcagacttt tgaaccgctt
    14461 attgatgtac aaacccgcta ctggtttaac ccggcggcga ttagccagca cttcattatc
    14521 cccggtgcgg tgaccattat catgacggtc atcggcgcga ttctcacctc gctggtggtg
    14581 gcgcgagaat gggaacgcgg caccatggag gctctgctct ctacggagat tacccgcacg
    14641 gaactgctgc tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg
    14701 tgtatgctgg tgtcagtgtt tattctcggc gtgccgtatc gcgggtcgct gctgattctg
    14761 ttttttatct ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg
    14821 attacccgca accagttcaa tgccgctcag gtcgccctga acgccgcttt tctgccgtcg
    14881 attatgcttt ccggctttat ttttcagatc gacagtatgc ccgcggtgat ccgcgcggtg
    14941 acgtacatta ttcccgctcg ttatttcgtc agcaccctgc aaagcctgtt cctcgccggg
    15001 aatattccag tggtgctggt ggtaaacgtg ctgtttttga tcgcttcggc ggtgatgttt
    15061 atcggcctga cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca
    15121 tcgcttatgg acgttaatcc gcaaagagtt gcagtcgctg ctgcgcgaac cgcaaacccg
    15181 cgcgattctg attttacctg tgcttattca ggtgattctg ttcccgttcg ccgccacgct
    15241 ggaagtgact aacgccacca tcgccatcta cgatgaagat aacggcgagc attcggtgga
    15301 gctgacccaa cgttttgccc gcgccagcgc ctttactcat gtgctgctgc tgaaaagccc
    15361 acaggagatc cgcccaacca tcgacacaca aaaggcgtta ctcctggtgc gtttcccggc
    15421 tgacttctcg cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg
    15481 gcgtaactcc aacagtgcgc aaattgccac caactacctg caacagatcg tcaaaaatta
    15541 tcagcaggaa ctgctggaag gaaaaccgaa acctaacaac agcgagctgg tggtacgcaa
    15601 ctggtataac ccgaatctcg actacaaatg gtttgtggtg ccgtcactga tcgccatgat
    15661 caccactatc ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg
    15721 tacgctcgat cagctactgg tttcgccgct caccacctgg cagatcttca tcggcaaagc
    15781 cgtaccggcg ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg
    15841 ggcgtatcaa atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta
    15901 cggtttatcg ctggtgggat tcggtctgtt gatttcatca ctctgctcga cacaacagca
    15961 ggcgtttatc ggcgtgtttg tctttatgat gcccgccatt ctcctttccg gttacgtttc
    16021 gccggtggaa aacatgccag tatggctgca aaacctgacg tggattaacc ctattcgcca
    16081 ctttacggac attaccaggc agatttattt gaaggatgcg agtctggata ttgtgtggaa
    16141 tagtttgtgg ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt
    16201 tagacgtaag gtgatgtaac ttcttatctt tcgccagcaa agacactacc gccgggccgg
    16261 caaggattgc cagccctgca attgccagca aaaagttgtt ttccagtacg cgggacaaca
    16321 gccacagcac aatcagcgct gcgccaaaat accaggtcgt ggtcagctct tccaacacgt
    16381 ctgccacctc acgccagcgt tgctcggggt ggcgctgaaa caccaacatc atcactaccg
    16441 tgacacaata cagtgcgcat aatcccacgc cgacgtgaac cagagtctta ctcatccagc
    16501 ccaccaccag cagcgccact accagtaaat gcaacataat ctgccagcaa cttagacccg
    16561 ttgcgacacg aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac
    16621 ttactcagag taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg
    16681 actacactat ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca
    16741 acaattttcg ttcaaggtgc tcaccatcaa cattcaaaaa ggttttaccg cgtttaaccg
    16801 acgcttcatt ttgccggaac ttcgcgacgc ggtgcgtacc gtcagcgccg atattgtttg
    16861 cctgcaggaa gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga
    16921 tacctcgcac tacgagtttc tcgccgacac tatgtggagc gattttgcct acggtcgcaa
    16981 tgccgtatac ccggaagggc atcacggcaa cgccgtactg tcgcgttatc ccattgaaca
    17041 ttatgagaat cgcgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg
    17101 cattgtgccg ccgatgaccg gaaaagcgat tcatgtgatg tgcgtacatc tgggcctgcg
    17161 tgaggcgcac cgtcaggcgc agcttgcgat ggggtgaatg agctaccgga cggcgaaccg
    17221 gtattggtgg cgggtgattt caatgactgg cggcaaaaag ctaatcatcc gttaaaagtg
    17281 caggccggac tggatgagat ttttacccgc gcccacggac gcccggcgcg cacgtttccg
    17341 gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag cgccagcgcg
    17401 ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc tttaagtgcg
    17461 gagattcatt tatgaaatgt agctggcgcg aaggcaataa gatccagttg ctggaaaacg
    17521 gcgagcaata ttatcccgcg gtgtttaagg cgattggcga gacacaagaa cgcatcattc
    17581 ttgaaacgtt tatctggttt gaggataacg tcggcaaaca gctgcatgcg gcactactgg
    17641 cagcagcgca acgcggggtt aaagcggaag tcttgctgga tggctacggt tcgccggatc
    17701 tcagcgatga gtttgtcaat aaactgacgg cagctggcgt agtgttccgc tactacgatc
    17761 cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat cgcaaaattg
    17821 tggtgatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc gagcatatgt
    17881 ccagctacgg tccagaggct aaacaggatt acgcggtacg ccttgaaggg ccgattgtcg
    17941 aagatattct ccagtttgag ctggaaaacc tgcctggaca gagcgcggca cggcgctggt
    18001 ggcgacgtca tcacaaagcg gaagagaacc gccagccggg agaagcgcag gtattgctgg
    18061 tctggcgcga taacgaagaa catcgcgatg atattgaacg ccattatctg aaaatgctca
    18121 ctcaggcgcg gcgggaagtg attatcgcca acgcctactt cttccccggc tatcgatttt
    18181 tacacgcctt gcgtaaagcg gcacggcgcg gggtgcggat caaactgatc attcagggcg
    18241 aaccggatat gccgattgtc agagtcggtg cgcgcttgct gtataactat ctggttaaag
    18301 gcggcgttca ggtttttgag taccgccgcc gcccgctcca cggcaaagtg gcattgatgg
    18361 acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg tcactgaatc
    18421 tcgaagcaaa tgtcatcatc cacgatcgtc attttaacca gacgctgcgc gataatctga
    18481 acggcattat tgccgcagat tgtcagcagg tggatgaaac catgctgccc aaacgcacct
    18541 ggtggaacct gaccaaaagc gtgctggcgt tccacttttt acgccacttc ccggcgctgg
    18601 ttggctggca tccggcacac acaccacgtc tgacgcaggt tgatcccccc gcacaaccga
    18661 caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc tgatgagtaa
    18721 atcacacccg cgctagcgct tagcaaagaa gatcctcacc tggctgtttt ttatcgcggt
    18781 gattgtgttg ctggtggtct acgccaaaaa agtggactgg gaagaggtct ggaaggtcat
    18841 ccgcgactac aatcgcgttg cgctgcttag tgcggtcggg ctggtggtcg tcagctatct
    18901 gatttacggc tgctatgacc tgctcgcccg cttttactgc ggtcacaaac tggcgaagcg
    18961 ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacgctca gtacctgggt
    19021 cggcggcatt ggtatgcgct atcgtttgta ctcccgacta gggttaccgg gcagcactat
    19081 tacgcggatt ttctcgctca gtattaccac caactggctg ggctatattt tactggcagg
    19141 gattatcttt accgcaggcg tggtggagtt gccggatcac tggtatgtcg ctcaaactac
    19201 gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt ggttttgcgc
    19261 tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc cttcatggaa
    19321 attcgccctc actcagatgc tgatttccag cgttaactgg atggtaatgg gggcgattat
    19381 ctggctgtta cttggtcaaa gcgtgaacta tttctttgta ctgggcgtgt tactggttag
    19441 tagtattgct ggcgtcatcg tgcatattcc ggcggggatc ggtgtgctgg aagcggtgtt
    19501 tatcgcacta ctggctgggg agcatacctc gaaagggagc attatcgccg cgctgctcgc
    19561 ttatcgcgtc ctgtattact ttatcccgct gctgctggcg ctgatctgtt acctgctact
    19621 ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa atttagttaa
    19681 gttctttagt atctgcattc acgactaatg aaaaaaacgc gtatgccttt gccagacaag
    19741 cgttatagct atcgcggcaa gtatcccaaa gaagccgata aaatagtaat agagtgttaa
    19801 tgcacctgta aaagcgcaac gaaccggact ccacaactct tcactatggc attttcctgc
    19861 cagttcaagg agcttatttc gtccatgcga gataatgcct ggccataaca caatcactaa
    19921 gcaacttatt acccaaatgg gccgttcact cattaatgcc gcattcacga tgattaccag
    19981 ggccaaccct gtcaacgtca tcataatgat aaaacggtga gaaccaggat caacattaaa
    20041 taataaaccg gccagcatac tgattaaaaa catcgcgccc gcagtgctga acacaatgat
    20101 aatggcatat acaggaacta aacttgcaac gacaatcgcc accaaagaga gtaaaaacag
    20161 atatattacg cctgcgatga cggcatttat cttgttgata tccttactta agccatacat
    20221 acaaactaat aataacccta tggcgacata cattgttggt gaatcatatt tggttgccgt
    20281 aatttcgaga tacttagctc cccaggccag tcctgcggtt gccaccaaac ctaaagcggt
    20341 aatgagccat ataccgttta atatcctggt ctcgtgctga aaatctaatt tattgctgtt
    20401 ttgtgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
    20461 ataaatttaa acccacgcat acaaaatact tttgtattta tggatacgga aaacgcttcc
    20521 gctcggccat aaggcgtatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
    20581 agaaatcccg acacatggcc gggatattga tggtgaaaaa gaattaacgg cggttgccaa
    20641 aaattcgcag caacatcagg aacaggttga tgaagtccag atacaaggtt aacgcgccaa
    20701 gaatggaata tttgcgcagg ttcgacgtgt cgcgggtatc aatctgctca cccatatttt
    20761 tcaatttctg agtgtcatac gccgtcaatc cgacaaagac aatcacgccg atgtaggtaa
    20821 ctgcccacat caatgcttcg cttttcagcc agaagttgac cagcgatgcc agcacaatgc
    20881 cgattaacgc cataaacagc atattgccaa agccacttaa atcgcgcttc gtggtgtaac
    20941 cgtacaggct cattgcgccg aacatcccgg cagtaacgac gaaagtactg gcgatagaag
    21001 cagcggtata gacaatgaat atactggaaa gcgtaagacc cgtcagcgcc gaataaagca
    21061 taaagagcat cgtcgttaca cctgcgctca gcttttgaat catcgctgat aacacaataa
    21121 ccaatgctaa ttgcgcgatg atcagaccga ttaaaaagac acggttagtg aacaacagct
    21181 ccatcacggc cgcggaatta gccgcatacc aggcaacaaa tgcggtcagc aacaagccaa
    21241 cggtcatcca gccatagact tgcgccatat aagtttgcaa gccagcccgg ggttgtacga
    21301 ttgaatcaga acgtgggaat ctgtccatga cgatctcctg aagatataag gaatatctta
    21361 aggatactgc aaaatgatga ggctgtgcat cgacgcagcg taaacgcatg tactgagcgg
    21421 tgaaattgcc ggacgcagcg gtgccttatc cggctaacaa aaaactacca gcgttttgcc
    21481 gcctgctgat cgctctcccg agcttcaacc cagcggtcgc cttccggcgt ggcttcgcgc
    21541 ttccagaacg gtgcgcgggt tttgagataa tccataataa actgcccggc ttcaaacgca
    21601 ctgctgcgat gcgcactggt gacaccggca aaaacgattt catcgcccgg ccataattcc
    21661 ccgatgcggt gaatcacagt gacgcgcccc agcggccagc ggttacgcgc ttcatcaaca
    21721 atttctgcca gtgctttttc agtcattccc ggatagtgtt cgagggttaa tgctttgacg
    21781 ctgtcgccca ggttatggtt gcgcacctta ccagtaaagg tgactaccgc accgtcttcg
    21841 tcacgctccg ccagccacgg gtactcttct cctacgctga acggctgcgg accaacaaca
    21901 attttggttt ctgccatctt aacctccggt taccggcggg aagaaagcta cttcgtcgcc
    21961 gtcagtcagc ggatggtcaa aactcaccag cgtctggttg acggcagcca gtaatttgcc
    22021 atcttccagc gccagcgccc agcgatcgct ctgcgcagcc atgtgctggc gtaacgcttc
    22081 aacagttggg aaatccgcag ccacttcggt tgcatctgtt cccaccaact cgcgcacctg
    22141 cgcgaaaaaa agaactttaa tcatccgctt ccaccttaaa gtcacccgac ttgccgccgc
    22201 tcttcgccag caaacgtacc ggaccaatca ccatatcttt ttgcaccgct ttgcacatgt
    22261 cataaatggt cagcgccgcc acggaggccg cggttaatgc ttccatttcg acaccggttt
    22321 tcccggtcag gcggcataag gtttctatac gcacccgatt gtgctccggc tcggcctgta
    22381 aattgacttc aactttgctg agcatcagcg gatgacagag cgggatcaga tcccaggtgc
    22441 gttttgccgc ctgaataccg gcaatacgcg cagtggcaaa tacgtcgcct ttgtggtggc
    22501 gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt gacaaaggct tccgcccgcg
    22561 cttcacgcac ggtttccgct ttggcggaga catccaccat gtgcgcttcg ccagcggcgt
    22621 tgatatgggt cagttgcgac atacttattt cttcaaatgt ggatggaaat tacacggacg
    22681 cgtacgggca tccagctgcg gcgcgatgat attttcccat gcggtacggc acgctttggt
    22741 cgaacccggc atggcgaaaa tcagcgtttt gttggcgacg cccgcaaccg cacgagattg
    22801 caacgtggaa gtgccaatct cttcaaacga caacatccgg aacacttcac caaaaccttc
    22861 aacttcacgg tcgaacaacg gcagcaatgc ttcgggagcc tgatcacctt ccgtcaggcc
    22921 agtaccaccc gtaatcaata ccacttgtac atcgtcgctg gcgatccacg cagatacctg
    22981 agcgcgaata gcgtagcggt tttctttcac aatggcttta tcgacaacgt gatggcccgc
    23041 ttcttgcgcc gaatcgcgca gatagtgacc ggaggtatcg tcttcttcac cgcgacgatt
    23101 agaaaccgta agaatagcaa tacgggtcgg gataaattca gtgcttacct gactcatctg
    23161 atctctcctt ttgacgtttt agccgccaat gtacgataag ttttgcgtaa taccggtgtt
    23221 gttttgatgc aggaaatggg tctgtttctt ctcccgcagc gccgctgaaa tacgcgcttc
    23281 cagcgcctgt tgctgggtat cgtcttccag cagatcgcgc aggttaacgc cgccttcacc
    23341 aaacaggcag agatggagtt taccaatgga ggaaacgcgc aggcggttgc aagtggcgca
    23401 gaagtctttt tcatacggca tgataaggcc aatctctccg gcgtaatctg gatggcaaca
    23461 gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg atccagccgc gacgcagtag
    23521 ctcgtcacgc agaacctgac cagagatgtg atgcttacgg aagagctcgc tgccctcgcc
    23581 cgtttccatc agttcgatga aacgcagctg gatagggcga tgctggatcc agttcagaaa
    23641 ggtgtcgagc tggtgatgat taacatcacg catcagcacg gtattgactt tgaccttctc
    23701 aaaaccggcc tcaaatgcag catcaatccc tgccatgacc tggttgaatt tatcctgccc
    23761 ggtaatagcg tgaaactggc gggcgtccag actgtcgaca ctgacgttaa tgccagtaag
    23821 tcccgcatcg cgccagttcg ccacatcgcg ttccagacgg taaccattgg tggtgaccgc
    23881 aatctggcgg atagcgtcgt tttcccgcac agcggcgatg atatcggtaa agtcgcggcg
    23941 taaagacggc tcacctcctg tcaggcgcac tttttcggtg cccagactgg cgaaggcgcg
    24001 cgtaacccgg cgaatttcat cgacggtaag aaagccttta ttggtgacgc cgctcggttt
    24061 gtagccatcc ggcaggcagt aggtgcaacg aaagttacac acatcggtaa tcgacaggcg
    24121 caagtagtaa aacttacgcg caaatgcatc agtcagttgt gaagccatgt acacctttcc
    24181 agatacggga ggcgaagtca tttcttcctt cgccctggtg gcaatcttta cgagcattgt
    24241 cacggccaaa gcaccgtatc agttgaccca ggtgcagagg ctagagtgtt tagtggttaa
    24301 gccgatacta gcgtgtaaat gagattttta ccatccacac tttcgctatg caatcatgta
    24361 tatagcgtca tgatcgtgct atttcgctac agaataaggg aaaatggcag ttttactttg
    24421 atatacatca tcagggaggc atagcgtgca tcgtccttta gaggtagaag aaaatagttt
    24481 gtatcctcaa tattggcagg ttaattgctg tttccccgca atttgcgcta ctgtagcgcg
    24541 gataatctga ctccaggaaa ctatatgcgc aatcgtacgc tggctgatct tgatcgtgtc
    24601 gttgctctcg gcggagggca tggactggga cgcgttctct catcactttc gtctttgggt
    24661 tctcgtttaa cgggtatcgt caccaccacg gataatggtg gctcgacggg gcgtattcgc
    24721 cgttcagaag gcggcattgc ctggggcgat atgcgcaact gcctcaacca actgataacg
    24781 gaaccgagcg tcgcctccgc gatgtttgaa taccgttttg gcggcaatgg cgaactttcc
    24841 gggcacaacc ttggaaactt gatgttaaag gcgctggatc accttagcgt gcggcctctg
    24901 gaagccatca atttaattcg taatctgctg aaagtggata cgcatttgat tccaatgtca
    24961 gagcatcccg tcgatctgat ggcgattgac gatcaggggc atgaagttta cggcgaggtc
    25021 aatatcgacc agttaactac gccgattcaa gagttattat taacgccgaa tgtacccgca
    25081 acgcgtgagg cagttcacgc tatcaatgaa gcggatctca tcattattgg gcctggcagt
    25141 ttttatacca gcctgatgcc aattctgctg ctgaaggaaa tcgcccaggc attacgccgc
    25201 acgccagcgc cgatggttta tatcggcaat ctggggcgtg agttgagttt acctgcggct
    25261 aatttgaagc tggaaagcaa gctggcaatt atggagcagt atgttggtaa aaaagtcatt
    25321 gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg tgaaagagcg gattgtgatc
    25381 caggaggtac tggaggccag cgatattccg tatcgtcatg accgccagtt gttacataac
    25441 gcgctggaaa aggcgttaca ggctttaggt taacggaaat ccgaaggaaa attccggctt
    25501 cctattgaag acaaagtgcg cgttgtttat gccggatgcg gcgtgaacgc cttatccggc
    25561 ctacaaaccg cgcaaattca atatattgcg gagaaaatgt aggcctgata agcgtagcgc
    25621 atcaggctgt tttccgtttg tcatcagtct tcttcgctat cctgttacga tgcggcaata
    25681 aacaactcac gcaactgatg caactggtca cgaatttgtg ccgcttcttc gaactccaga
    25741 ttctgcgcgt gttgcatcat cagcccttcc agttcatgga ttttctgctg caacgctttc
    25801 ggcgacatat ccatcggcac attatccggc tcaacaatcg ggcgcgattt tcctctgccc
    25861 ttcgctttgg ttttggcaat gttctgcccc agcgccagga tatcgaccac tttcttgttc
    25921 aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc acggcgacgt
    25981 tcggtttcgc caatcgcttt cgccattgat ggggtgatct tatcgccgta gagaatcgct
    26041 ttaccgttaa cgttacgtgc cgcacgacca atggtctgga tcaacgaacg ttcggaacgc
    26101 aggaagcctt ctttgtcagc gtcgaggatc gccaccagcg acacttccgg catatccaga
    26161 ccttcgcgca gtaagttgat ccctaccagc acgtcgaact cacccagacg caagtcgcgg
    26221 ataatctcca tacgttcgac ggtgtcgata tctgagtgaa gataacgcac gcgctcgcca
    26281 tgttcttcga gatattcggt aagatcttcc gccatccgct tggtcagtgt ggtgaccagt
    26341 acgcgttcgt taattgccgc tcgctgacga atctccgaaa gaagatcatc aacctgtgtc
    26401 gccaccggcc gcacttcgat aatcgggtca agcaatccgg ttggacgcac cacctgatcc
    26461 accacatcgc cgccggattt ttccagctcg taattacccg gcgtcgccga aacatagatg
    26521 gtttgcggcg ctaatgcttc gaactcttca aacttaagcg gacgg
//
LOCUS       DADLTD010000034        25949 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLTD010000034 DADLTD010000000
VERSION     DADLTD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.034"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="JN701_001149"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="JN701_001149"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7363665.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2663)
                     /gene="btuB"
                     /locus_tag="JN701_001150"
                     /pseudo
     CDS             complement(815..2663)
                     /gene="btuB"
                     /locus_tag="JN701_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2717..2907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3021..4121
                     /gene="trmA"
                     /locus_tag="JN701_001151"
     CDS             3021..4121
                     /gene="trmA"
                     /locus_tag="JN701_001151"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY7363666.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4161..4520)
                     /locus_tag="JN701_001152"
     CDS             complement(4161..4520)
                     /locus_tag="JN701_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY7363667.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="JN701_001153"
     CDS             complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="JN701_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY7363668.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5491..6891
                     /gene="sthA"
                     /locus_tag="JN701_001154"
     CDS             5491..6891
                     /gene="sthA"
                     /locus_tag="JN701_001154"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY7363669.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="JN701_001155"
     CDS             complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="JN701_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY7363670.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAMPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8058..9431)
                     /gene="argH"
                     /locus_tag="JN701_001156"
     CDS             complement(8058..9431)
                     /gene="argH"
                     /locus_tag="JN701_001156"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY7363671.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9492..10268)
                     /gene="argB"
                     /locus_tag="JN701_001157"
     CDS             complement(9492..10268)
                     /gene="argB"
                     /locus_tag="JN701_001157"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY7363672.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10276..11280)
                     /gene="argC"
                     /locus_tag="JN701_001158"
     CDS             complement(10276..11280)
                     /gene="argC"
                     /locus_tag="JN701_001158"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY7363673.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11434..12585
                     /gene="argE"
                     /locus_tag="JN701_001159"
     CDS             11434..12585
                     /gene="argE"
                     /locus_tag="JN701_001159"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY7363674.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            12937..15588
                     /gene="ppc"
                     /locus_tag="JN701_001160"
     CDS             12937..15588
                     /gene="ppc"
                     /locus_tag="JN701_001160"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY7363675.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15771..17504
                     /gene="cptA"
                     /locus_tag="JN701_001161"
     CDS             15771..17504
                     /gene="cptA"
                     /locus_tag="JN701_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY7363676.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17719..18570
                     /locus_tag="JN701_001162"
     CDS             17719..18570
                     /locus_tag="JN701_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7363677.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPTTTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASSLTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18557..18898)
                     /locus_tag="JN701_001163"
     CDS             complement(18557..18898)
                     /locus_tag="JN701_001163"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7363678.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18900..19778)
                     /locus_tag="JN701_001164"
     CDS             complement(18900..19778)
                     /locus_tag="JN701_001164"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY7363679.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19744..22041)
                     /locus_tag="JN701_001165"
     CDS             complement(19744..22041)
                     /locus_tag="JN701_001165"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7363680.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22092..22412)
                     /locus_tag="JN701_001166"
     CDS             complement(22092..22412)
                     /locus_tag="JN701_001166"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7363681.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22427..23506)
                     /locus_tag="JN701_001167"
     CDS             complement(22427..23506)
                     /locus_tag="JN701_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY7363682.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23815..>25949
                     /gene="ptsP"
                     /locus_tag="JN701_001168"
     CDS             23815..>25949
                     /gene="ptsP"
                     /locus_tag="JN701_001168"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7363683.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMT"
BASE COUNT         6383 a         7188 c         6435 g         5943 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagcttctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ttctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacta gccgggttcc ctctggcagc acttgtaaca gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaactgtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcag tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcaccgcg gtgacaattg ccaccactcg
      661 ctcaacaata aacgcttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcactgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccataa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acggatatcg agcaacggtg aaccgggaga ataatacgcg tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaacatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcacgc aatagggaac tggctaaggt cggcagaacc actcaccccc
     2341 gccagattca ggcgtacgcc atcaattaac accaacacat gactggcatt tgtaccgcga
     2401 ataaaaatag atgagagctg acctgaaccg ccgttttggg tgatatcgac gcccggaaga
     2461 cggcgcagca catcattgac cgaggtcgac tgccagcggt cgatatcctg acgggtcaca
     2521 acggtggttg gtgcaagcac agtgctgcgc ggctgttcaa aacggttagc agtaacgacg
     2581 agagtatccg ggctggtatc ctgtgcccaa gcggaaaagg ctgtgacaga acacgccgtc
     2641 agcagcgaag cttttttaat cattgtaaag catccacaat agaagaagga tgccgcaggt
     2701 ttcatcaata ttacgcgatg atgagacgtt ggccggcagg tcttcgggct tggaggggta
     2761 tctaagatac taagagatga tgacttccca ccggatggca gtgtccgcat aacgcaatca
     2821 tcgcaccttt ccttaccgct gcgcgtcagc tccagattcg cactggattc cctattaact
     2881 cacaggaccg gcaagtggat gctacaggtt gtaacaagtt actgtccaga cgtagctcac
     2941 aaataggaat tcatcaagat ctggacatct gatgagcaat ccctacaatc gccgcgtact
     3001 ttaatttttc aggatacatc atgacccccg aacaccttcc aacagaacag tatgaagcgc
     3061 agttagccga aaaagtggta cgtttgcaaa gtatgatggc accgttttct gacctggttc
     3121 cggaagtgtt tcgctcgccg gtcagtcatt accggatgcg tgcggagttc cgcatctggc
     3181 acgatggcga tgacctgtat cacatcattt tcgatcaaca aaccaaaagc cgtatccgcg
     3241 tggatagctt ccccgccgcc agtgaactta tcaaccagtt gatgacggcg atgattgcgg
     3301 gtgtgcgtaa taatcccgtt ctgcgccaca agttgttcca gattgattac ctcaccacgc
     3361 tgagtaatca ggcggtggtt tccctgctat accataagaa gctggatgat gagtggcgtc
     3421 aggaagcgga ggccctgcgc gatgcactgc gcgcgcagaa tctgaatgtg catctgattg
     3481 gtcgggcaac gaaaaccaaa atcgagctgg atcaagatta catcgacgag cgtctgccgg
     3541 tcgcagggaa agagatgatc taccgtcagg tagaaaacag ctttacccag ccgaacgcgg
     3601 cgatgaatat tcagatgctg gaatgggcgc tggacgtaac caaaggctca aaaggcgatt
     3661 tactggagct gtactgcggc aacggtaact tttcattagc gctggcgcgc aattttgatc
     3721 gggtattagc caccgaaatc gctaagccgt cggttgctgc tgcgcaatac aacatcgcag
     3781 ctaaccatat tgataacgta caaattattc gtatggcggc agaagaattt actcaggcga
     3841 tgaatggtgt gcgcgagttt aaccgcctgc aagggatcga cttaaagagt tatcagtgcg
     3901 aaaccatttt tgtcgaccca ccgcgcagcg gtctggacag tgaaaccgag aaaatggtgc
     3961 aggcgtatcc gtgtattttg tacatctcct gtaacccgga aacgttatgc aagaatctgg
     4021 aaacattaag ccagacgcac aaggtagaac gtctggctct gtttgatcag ttcccctaca
     4081 cgcaccatat ggagtgcggc gtattactga ccgcgaagta aaacccatgc cggatgcgcc
     4141 agcatccggc ataataccga ttactcagca acttcctgct tacggttacg catcttcgcg
     4201 ccaatccaga acaccataat gacggagagt actgccggga agaagttaga gccgatatcc
     4261 ggatattccg cacgaactac cgtgctgtac agcagtacgc cgagaataaa acaggcagct
     4321 gccagacccg gcaagcctac cggcatagtg cgattaagat aacgttgatg caggcagtaa
     4381 accgtcagca ccagggaaat aatcgggaat acagaaaatg gcacaatgga gctaaacagc
     4441 gctgcgaaag taccattaat cgataagcca gcaactaacg ccagcagcag cgtacctcta
     4501 tcttgatttg cttgtttcat tactcgtcct tcacatttcc cggaataatt gcggttttct
     4561 cttgttcacg gcgataccag taataagccc ctttcgaaat cattcgcagt tgcagtacca
     4621 gtcgctcttc taattgccga cgttgttcga cgccgacgtc caacgcctcg gcacccgcac
     4681 tgaagacaat tgtcaccatt gcttcggctt gcgcttcagt aaacgcacgc ggcatatggt
     4741 tttcgagttc cagatagtcc gcaagttccg caatgaagtg ctgaatttca cgcgcaacgg
     4801 cggcacgaaa cgcagcggag gtgccggagc gttcccgcaa taataaccgg aaggcgttag
     4861 gattattacc gatgaactcc ataaatgtgg agaccgaggt gcggatcaca ctcccgcctt
     4921 tggcgatacg ctgacgcgcc tggcgcatga gttggcgtag cattaaaccg ctctcatcaa
     4981 ccatggtcag acccagttcg tctacgtcgc ggaaatgccg ataaaaagag gtgggagcaa
     5041 tgcccgcttc acgcgccact tcacgcaaac tcaggctggc gaagctgcgt tcagcactta
     5101 attggctaaa tgcggcttcc accagcgaac ggcgggtttt ttctttttgt tgcgctctta
     5161 tgcccatcac gatgtctgaa tccttgccaa aagtactgct ggcactatac cagagaatga
     5221 acatactgga ttgctccagt attgtgaatg aacggtaacg caataaaaca tatttactgg
     5281 ccggttcttt tacgtacagc ggaaacctgc cgcttaaacg gagagtatcg tcgataaaaa
     5341 tccaataaaa cgtcagggca aaagtaagaa acagacaaag caaaggccgc tcaggatata
     5401 gccagataaa tgacggggat caattggctt acccgcgata aaatgttacc attctgttgc
     5461 ttttatgtat aagaacaggt aagccctacc atgccacatt cctacgatta cgatgccata
     5521 gtaataggtt ccggccccgg cggcgaaggc gctgcaatgg gcctggttaa gcaaggtgcg
     5581 cgcgtcgcag ttatcgagcg ttatcaaaat gttggcggcg gttgcaccca ctggggcacc
     5641 atcccgtcga aagctctccg tcacgccgtc agccgcatta tagaattcaa tcaaaaccca
     5701 ctatacagcg accattcccg actgctccgc tcttcttttg ccgatatcct taaccatgcc
     5761 gataacgtga ttaatcaaca aacgcgcatg cgtcagggat tttacgaacg taatcactgt
     5821 gaaatattgc aggggaacgc tcgctttgtt gacgagcata cgttggtgct ggattgcccg
     5881 gacggcagcg tcgaaacact aaccgctgaa aaatttgtta ttgcctgcgg ctctcgtcca
     5941 tatcatccaa cagatgttga tttcacccat ccacgcattt acgacagcga ctcaattctt
     6001 agcatgcacc acgaaccgcg ccatgtactt atctatggtg ctggagtgat cggctgtgaa
     6061 tatgcgtcga tcttccgcgg tatggatgta aaagtggatc tgatcaacac ccgcgatcgc
     6121 ctgctggcat ttctcgatca agagatgtca gattctctct cctatcactt ctggaacagt
     6181 ggcgtagtga ttcgtcacaa cgaagagtac gagaagatcg aaggctgtga cgatggtgtg
     6241 atcatgcatt tgaagtcggg taaaaaactg aaagctgact gcctgctcta tgccaacggt
     6301 cgcaccggta ataccgattc gctggcgtta cagaacattg ggctggaaac tgacagccgc
     6361 ggacagctga aggtcaacag catgtatcag accgcacagc cgcacgttta cgcggtgggc
     6421 gacgtgattg gttatccgag cctggcatca gcggcctatg accaggggcg cattgccgcg
     6481 caggcgctgg taaaaggcga agccaccgca catctgattg aagatatccc taccggcatt
     6541 tacaccatcc cggaaatcag ctctgtgggc aaaaccgaac agcagctgac cgcaatgaaa
     6601 gtgccatatg aagtgggccg cgcccagttt aaacatctgg cacgcgcaca aatcgtcggc
     6661 atgaacgtgg gcacgctgaa aattttgttc catcgggaaa caaaagagat tctgggtatt
     6721 cactgctttg gcgagcgcgc tgccgaaatt attcatatcg gtcaggcgat tatggaacag
     6781 aaaggtggcg gcaacactat tgagtacttc gtcaacacca cctttaacta cccgacgatg
     6841 gcagaagcct atcgggtagc tgcgctaaat ggcttaaacc gcctgtttta acactttatc
     6901 gaaatggcca tccattcttg cgcggatggc ctctgccagc tgctcatagc ggctgcgcag
     6961 cggtgagcca ggacgataaa ccaggccaat cgtgcggcga ggttccggct taatacacgg
     7021 caaataaaca accccatcgc gtttgcgctc cggcggcata gccagcgcag gcagtaaagt
     7081 gatcccgcta cctgccgcca ccatgttacg cagtgtctcc aggctggtcg cgcggaagtg
     7141 tgtatcttca tccgccccgg cttcaaagca gaaacccatt gcctgatcgc gcaaacagtg
     7201 accatcttcc agcatcagca gtttttcccc tgccagatcg gccatcggta cgcattcgcg
     7261 gttcgcccac gggtgatctt catagatagc cagcaacatt ggctcatcaa acaacggcac
     7321 ttcaatgaat gcttcgctct ctttcaccag cgcgaggatc acgcaatcga gtttgccgct
     7381 gtccagttgc gccagtaact ggtgggtctg tgcttcatgc agatacattt ccagctttgg
     7441 aaatgtctgg tgcagcatcg ggataatatg cggcagcagg tacggtccaa ccgtaggaat
     7501 caaaccaata tgcagcggtc cggacatcgt ctcgccctgc tggcttgcca tctctttaag
     7561 gactttcacc tcacgcagca cggtacgcgc ctgatccacc agcagcattc ccgcctgggt
     7621 gaacaacact ttacggctgg tccgctccag caacatcacg cccagctcat cttccagctt
     7681 acgaatttgg ccgctaagcg tcggctggct aacgtggcag gaatctgccg cacgccgaaa
     7741 atggcggtgt tcagccaatg ccaccaggta ctcaagatca cgaatattca ttatccatcc
     7801 tccatcgcca cgatagttca tggcgatagg tagaatagca atgaacgatt atccctatca
     7861 agcattctga ctgataattg ctcacagaaa cggagcggca cctcttttaa cccttgaagt
     7921 cactgcccgt ttcgagagtt tctcaactcg aataactaaa gccaacgtga acttttgcgg
     7981 atctccagga tccgcttttt tttgccataa aaaagcccgg cgataagcca ggctcaaatt
     8041 tgtacatata aatgttctta ccccaaccgc gcctgcgcaa aagcaatcgc ttgggccacc
     8101 tgctgcggtg agacgccgcc ttttgccgca cgcttgtcga ggcacgattg tagcgacaga
     8161 atcggataga catcttcgcc aatcacgtgg ctgaatttct gcaactcgtc gagcggcaga
     8221 tcttccagcg gtttgccctg acgaatggct tccaccaccg cttcacccac gatatggtgc
     8281 gcctcgcgga aaggtacgcc tttcgccacc aggtaatccg ccagttcggt ggcgttggcg
     8341 taaccctgct gagccgcttc ctggcaacgt ggacgtttca cctgaatgcc gtccagcacc
     8401 agcgccgcca tatgcaggca gtccagccag gtatcgaacg cgtcgaacag accttctttg
     8461 tcttcctgca tatctttgtt gtaagccagc ggcaaacctt tcagcgtcat catcatgccg
     8521 gttaacgcac cctgcacccg accgcattta ccgcgaatca gctccagcgc atccgggttt
     8581 ttcttctgcg gcattaatga tgaaccggaa gtcacgcggt cagaaagctc cacaaacccc
     8641 gcttcgccgg tgttaaagaa aatcagatct tcagcaaaac gcgacagatg caccatgccg
     8701 atagcggcag ccgacagcag ttccaacaca tggtcacggt cagaaacgct gtccagactg
     8761 ttacgggtcg ccgaagcaaa gcccagccag cctgctaact gttcacggtc gatttcatag
     8821 gccgttcccg ccagcgcgcc acagcctaat gggctgacat ccagacgctt aagcgcatcc
     8881 tgcaaacggc tttcatcacg cgccagcatc tcaacatagg ccaggcacca gtgcgcgaac
     8941 gtcaccggct gggcgcgttg caggtgagtg taacctggca ttaccgcgtc ctgattgttt
     9001 tgtgcggttt ccaccagcgc cgattgcagc tgccggttag ccgtcagtaa ctcgctaacg
     9061 gtatctttgc accacagttt caggtcagtc gctacctgat cattacggct acgcccggta
     9121 tgcagctttt tgcctaactg gcccactttg tcgatcagtt tgccttccac ccagctatgg
     9181 atatcttcgg cgtcgctttc aaggatttgt tgtggcctgg cgcgaacatc ttccagcaac
     9241 acgttcagcg cctcttccag ttgcgcctgc tcttctgcgg ttaacacgcc gaccgttacc
     9301 agggctttgg accaggccac agagccaaca atatcctgct ccgccagacg gtaatcaaag
     9361 cgcagtgagt cgttgaattg tttgaaccgt tgatctgctg cctgggtaaa acgcccgccc
     9421 caaagtgcca taactctgtt tccttatttt gaaattcaat gccggaaagc tgcgcctccg
     9481 gctaacaaaa cttaagctaa aatccgcgta cccatcggca taccgttaaa cagtgccgga
     9541 agctgctccg catgacgcca ggaggcgata tctaccggac ggcccagcgt gcgggccgca
     9601 tccagcgccg cgttcacttt cactatcatg ccgtcagtaa taatgccctg ctcaatcagt
     9661 tgttctgctt tcgcggcggt catttcggca atgcgttgcc ctttgccgtc gagaatgccg
     9721 ctgacgtcgg agagcaaaat cagatccgcg cccagcgttg ccgccagcgc cgttgccgcc
     9781 tggtcggcat tgacgttcat cagttgccct tcgtctgtta cgccaatgga gctgaccacc
     9841 ggcagataac cgttctccag caaggagttg ataagcttag gcgaacctgg ctgcgccagt
     9901 ccaacatgac ctaactcttc atcaagctgg gtcactttga cgctgtcgcc atcaccgaga
     9961 aacaaaccta cagccgcaat ctgatgtttc ttcgcccatg ccaacagggt tttatttgcc
    10021 gttcccgcca gtgctccggt gataatgtct atctgatcag caggcgtcac ccgcaggccg
    10081 tttttctttt tcaccggcag attcagccct ttcatcagct catccaccac gcaaccgccg
    10141 ccgtgcacaa tcaccagcgg acgctgatgt gactcacgat aattcaccag tgcgctaaac
    10201 agacgttcca gcgcctcttc actatccagc agtacgccgc ccagtttgat aattaatgga
    10261 ttcatcattg cacccttaaa taagagactg cgtttccgca tagccgaaac gaatattggc
    10321 gcactgtacc gcttgtgccg ccgcgccttt cagtaagttg tcttcggtcg ccacaataat
    10381 cagatgctcg ccctgaacgg caaacccgat atcgcaaaat ggcagcccaa cgacattttt
    10441 cagcgccgga acgcctttgt catacagccg caccagcggt ttatgggcat acgcctgctg
    10501 taacacttgc gcgacttgcg cctgggtcac acccgatttc aggcggcagg taatggtttc
    10561 gagaatgccg cgcgggaaat tgcccagatg tggggtgaag ataacgtcag caccgaggtg
    10621 tgtggcgatc tctggttgat ggcgatgagt aaagacgcca tacggttgca ggctaacttc
    10681 acaaaaacta tttgaaatcg ctgccttacg tcctgcaccg ctcacgccgc tggtggcgtt
    10741 aatcgccggc cactgattga ggtcaagaag atcggcatca atcaacggtt tcagcgccag
    10801 ctgtgccgcc gtcggataac agcctggcac cgcaatcaaa ttcgcttctt ttaatttatt
    10861 accgcaccac tccgccagac cgtaggctgc ctgttccagc agttccgggt attgatgggt
    10921 aaagccgtaa tatttttcat agaaggtggc gtcgttaaca cgaaacgcgc cggaaaggtc
    10981 gaacaccacg cagcccgctt caagaaactg cggcgctaaa tcgtggctaa cttcatgggc
    11041 ggtggcgaga aacactacgt ccacccctgg gctaaactcg ctgatatccg acatcggctg
    11101 caacggcagc tcaacgatgc cttttagctg cggatgcaaa tcggagatta actttcccgc
    11161 atcattgctt tgcgctgaaa cagtcaaagc ggttatgttc atatgcggat ggcgatttac
    11221 ataggtcact agctctgcgc cagcgtagcc gctggcaccc acaatcagcg tattcaacat
    11281 cggggctatt caccttctta tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg
    11341 gctggtgggt tttattacgc tcaacgttag tgtattttta ttcataaata ctgcatgaat
    11401 attgatacta tcatgaccag aggtgtgtca acaatgaaaa acaaattacc gccatttatc
    11461 gagatttacc gcgctctgat tgccacacct tcaataagcg ctacggaaga ggcactcgat
    11521 caaagcaatg cagatttaat cactctgctg gcggactggt ttaaagattt gggcttcaat
    11581 gtggaagtgc agcctgttcc aggaactcgc aacaaattca atatgctggc aagtatcgga
    11641 cagggggctg gcggcttgtt gctggcgggg cataccgata cggtgccatt tgatgacggt
    11701 cgctggacgc gcgatccgtt tacactgacg gagcatgacg gcaagcttta cggcttaggc
    11761 accgccgaca tgaaaggctt ttttgcgttt atccttgatg cgctacgcga tatcgacgtc
    11821 acgaaactga aaaaaccgct ctacattctg gcgactgctg atgaagaaac cagtatggcc
    11881 ggagcgcgtt attttgccga aactaccgcc ctgcgcccgg attgcgccat cattggcgaa
    11941 ccgacgtcac tacaaccggt acgcgcacat aaaggtcata tctctaacgc catccgtatt
    12001 cagggccagt cggggcactc cagcgatcca gcacgcggag ttaacgctat cgaactaatg
    12061 cacgacgcca tcgggcatat tttgcaattg cgcgataacc tgaaagaacg ttatcactac
    12121 gaagcattta ccgtgccata ccctacgctc aacctcgggc atattcacgg tggcgacgct
    12181 tctaaccgta tttgcgcttg ctgtgagttg catatggata ttcgtccgct gcctggcatg
    12241 acactcaatg aacttaatgg tttgctcaac gatgcattgg ctccggtgat cgaacgctgg
    12301 ccgggtcggc tgacggtcga cgagctgcat ccgccgatcc ctggctatga atgcccaccg
    12361 aatcatcaac tggttgaagt ggttgagaaa ttgctcggag caaaaaccga agtggtgaat
    12421 tactgtaccg aagcgccgtt tattcaaacg ttatgcccga cgctggtgct cggtccgggt
    12481 tccatcaatc aagctcatca gcctgatgaa tatctggaga cgcggtttat taaacccacc
    12541 cgtgagttga ttatccaggt aattcaccat ttttgctggc attaaaacgt aggccggata
    12601 aggcgctcgc gccgcatccg gcaccgttgc caaactccgg tgccgcaata atgtcggatg
    12661 cgatgcttgc gcatcttatc cgacctacac ctttggtgtt atgtatggcg attttttaac
    12721 atttccataa gttacgctta tttaaagcgt cgtgaattta atggcgtaaa ttcctgctat
    12781 ttattcgttt gctgaagcga tttcgcagca tttgacgtca ccgcttttac gtggctttat
    12841 aaaagacgac gaaaagcaaa gcccgagcat attcgcgcca atgcgacgtg aaggatacag
    12901 ggctatcaaa cgataagatg gggtgtctgg ggtaatatga acgaacaata ttccgcattg
    12961 cgtagtaatg tcagtatgct cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga
    13021 gaacacattc ttgaacgcgt agaaactatc cgtaagttgt ccaaatcttc acgcgctggc
    13081 aatgatgcta accgccagga gttactcacc accttacaaa atttgtcgaa cgacgagctg
    13141 ctgcccgttg cgcgtgcgtt tagtcagttc ctgaacctgg ccaacaccgc cgagcaatac
    13201 cacagcattt cgccgaaagg cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg
    13261 cgtaaactga aaaaccagcc ggaactgagc gaagacacca tcaaaaaagc agtggaatcg
    13321 ctgtcgctgg aactggtcct cacggctcac cctaccgaaa ttacccgtcg tacactgatc
    13381 cacaaaatgg tggaagtgaa cgcctgctta aaacagctcg ataacaaaga tatcgctgac
    13441 tacgaacaca accagctgat gcgtcgcctg cgccagttaa tcgcccagtc atggcatacc
    13501 gatgaaatcc gtaagctgcg tccaagcccg gtagatgaag ccaaatgggg ctttgccgta
    13561 gtggaaaaca gcctgtggca aggcgtacca aattacctgc gcgaactgaa cgaacaactg
    13621 gaagagaacc tcggctacaa actgcccgtc gaatttgttc cggtccgttt tacttcgtgg
    13681 atgggcggcg accgcgacgg caacccgaac gtcactgccg atatcacccg ccacgtcctg
    13741 ctactcagcc gctggaaagc caccgatttg ttcctgaaag atattcaggt gctggtttct
    13801 gaactgtcga tggttgaagc gccccctgaa ctgctggcgc tggttggcga agaaggtgcc
    13861 gcagaaccgt atcgctatct gatgaaaaac ctgcgttctc gcctgatggc gacacaggca
    13921 tggctggaag cgcgcctgaa aggcgaagaa ctgccaaaac cagaaggcct gctgacacaa
    13981 aacgaagaac tgtgggaacc gctctacgct tgctaccagt cacttcaggc gtgtggcatg
    14041 ggtattatcg ccaacggcga tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta
    14101 ccgctggtcc gtattgatat ccgtcaggag agcacccgcc ataccgaagc gctgggcgag
    14161 ctgacccgct acctcggtat cggcgactac aaaagctggt cagaggccga caaacaggcg
    14221 ttcctgatcc gcgaactgaa ctccaaacgt ccgcttctgc cgcgcaactg gcaaccaagc
    14281 gccgaaacgc gcgaagtgct cgatacctgc caggtgattg ccgaagcgcc gcaaggctcc
    14341 attgccgcct acgtgatctc gatggcgaaa acgccgtccg acgtactggc tgtccacctg
    14401 ctgctgaaag aagcgggtat cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc
    14461 gatgacctga acaacgccaa cgatgtcatg acccagctgc tcaatatcga ctggtatcgc
    14521 ggcctgattc agggcaaaca gatggtgatg attggctatt ccgactcagc aaaagatgcg
    14581 ggcgtgatgg cagcgtcctg ggcgcaatat caggcacagg atgcattaat caaaacctgc
    14641 gaaaaagcgg gtattgagct gacgttgttc cacggtcgcg gcggttccat tggtcgcggc
    14701 ggtgcacctg cccatgcggc actgctgtca caaccgccag gaagcctgaa aggcggcctg
    14761 cgcgtaaccg aacagggcga gatgatccgc tttaaatatg gtctgccaga aatcaccgtc
    14821 agcagcctgt cgctttatac cggggcgatt ctggaagcca acctgctgcc accgccggag
    14881 ccgaaagaga gctggcgtcg cattatggat gaactgtcag tcatctcctg cgatgtctac
    14941 cgcggctacg tacgtgaaaa caaagatttt gtgccttact tgcgctccgc tacgccggaa
    15001 caagaactgg gcaaactgcc gttgggttca cgtccggcga aacgtcgccc aaccggcggc
    15061 gtcgagtcac tacgcgccat tccgtggatc ttcgcctgga cgcaaaaccg tctgatgctc
    15121 cccgcctggc tgggtgcagg tacggcgctg caaaaagtgg tcgaagacgg caaacagagc
    15181 gagctggagg ctatgtgccg cgattggcca ttcttctcga cgcgtctcgg catgctggag
    15241 atggtcttcg ccaaagcaga cctgtggctg gcggaatact atgaccaacg cctggtagac
    15301 aaagcactgt ggccgttagg taaagagtta cgcaatctgc aagaagaaga catcaaagtg
    15361 gtgctggcga ttgccaacga ttcccatctg atggccgatc tgccgtggat tgcagagtct
    15421 attcagctac ggaatattta caccgacccg ctgaacgtat tgcaggccga gttgctgcac
    15481 cgctcccgcc aggcagaaaa agaaggccag gaaccggatc ctcgcgtcga acaagcgtta
    15541 atggtcacta ttgccgggat tgcggcaggt atgcgtaata ccggctaatc ttcctcttct
    15601 gcaaaccctc gtgcttttgc gcgagggttt tctgaaatac ttctgttcta acaccctcgt
    15661 tttcaatata tttctgtctg cattttattc aaattctgaa tataccttca gatatcctta
    15721 aggaattgtc gttacattcg gcgatatttt tttcaagaca ggttcttact atgcattcca
    15781 cagaagtcca ggctaaaccc ctttttagct ggaaagccct gggttgggca ctgctctact
    15841 tttggttttt ctctactctg ctacaggcca ttatttacat cagtggttat agtggcacca
    15901 acggcattcg cgactcgctg ttattcagct cgctgtggtt gatcccggta ttcctctttc
    15961 cgaagcggat taaaattatt gccgcagtaa tcggcgtggt gctatgggcg gcctctctgg
    16021 cggcgctatg ctactacgtc atctacgggc aggagttctc gcagagcgtt ctgtttgtga
    16081 tgttcgaaac caacaccaac gaagccagcg agtatttaag ccagtatttc agcctgaaaa
    16141 ttgtgcttat cgcgctggcc tatacggcgg tggcagttct gctgtggaca cgcctgcgcc
    16201 cggtctatat tccaaagccg tggcgttatg ttgtctcttt tgccctgctt tatggcttga
    16261 ttctgcatcc gatcgccatg aatacgttta tcaaaaacaa gccgtttgag aaaacgttgg
    16321 ataacctggc ctcgcgtatg gagcctgccg caccgtggca attcctgacc ggctattatc
    16381 agtatcgtca gcaactaaac tcgctaacaa agttactgaa tgaaaataat gccttgccgc
    16441 cactggctaa tttcaaagat gaatcgggta acgaaccgcg cactttagtg ctggtgattg
    16501 gcgagtcgac ccagcgcgga cgcatgagtc tgtacggtta tccgcgtgaa accacgccgg
    16561 agctggatgc gctgcataaa accgatccga atctgaccgt gtttaataac gtagttacgt
    16621 ctcgtccgta caccattgaa atcctgcaac aggcgctgac ctttgccaat gaaaagaacc
    16681 cggatctgta tctgacgcag ccgtcgctga tgaacatgat gaaacaggcg ggttataaaa
    16741 ccttctggat caccaaccag cagacgatga ccgcccgcaa taccatgctg acggtatttt
    16801 cgcgccagac cgacaagcag tactacatga accagcaacg tacgcagagt gcgcgtgaat
    16861 acgacaccaa cgtgctgaag ccgttccagg atgtgctgaa tgaccctgcg ccgaagaaac
    16921 tgatcatcgt tcatctgctg ggtacgcata tcaaatacaa ataccgctac ccggaaaatc
    16981 agggcaagtt tgatggcaat accgatcatg ttccgccagg attaagcgca gaagagctgg
    17041 aatcatataa cgattatgac aacgctaact tgtataacga tcatgtggtt gccagcctga
    17101 ttaaagactt taaagcggca gacccgaacg gattcctggt ttacttctct gaccacggtg
    17161 aagaggttta cgacacgccg ccgcacaaaa ctcaggggcg taacgaagac aacccgacgc
    17221 gccacatgta caccattccg ttcctgctgt ggacgtcgga aaaatggcaa gcgactcatc
    17281 cccgtgattt ctcacaggat gtcgatcgta aatacagcct ggcggaactg atccacacct
    17341 ggtcagattt ggcgggctta tcttacgacg gttacgatcc aacccgttca gtggtgaatc
    17401 cgcagttcaa agaaactacc cgctggattg gtaacccgta caagaaaaac gcgctgatcg
    17461 attacgacac tctgccgtat ggcgaccagg taggtaatca gtaattatca gccaggcgct
    17521 atttgccgga tgcgacgctt gacgcgtctt atccggccta cagatcgcgg cggtaggctg
    17581 gataaggcgt ttacgccaca tccggcaata caactgccgc aatgtttaca tcgaactccc
    17641 ctcaggccac acttttcagg tggccttttt taataatgcc agttactgtt ttcccgtcta
    17701 catacctaag ggaataacat gtatcacgac atcagctatc tgctctcccg cctgatcaac
    17761 ggcccgctgt ccctgcgcca gatttacttt gccagcagta acggcccggt gcccgatctt
    17821 gcgtatcagg tcgattttcc acggctggaa attgtgctgg aaggtgagtt tgttgatacc
    17881 ggcgctggag caacgttagt tcccggcgat gtgctgtacg ttgccgctgg tggctggaat
    17941 ttcccacaat ggaaaacacc cactaccacc tttagcgtgc tgtttggcaa acagcaactc
    18001 ggcttcagcg tcgtgcaatg ggatggcaaa caatatcaaa atctggcgaa gcaacacgtc
    18061 gcccgacgcg gcccacgtat aggttctttt ctgctacaaa cgctcaatga aatgcaaatg
    18121 cagccgcagg agcagcaaac ggcaaggctt atcgtagcca gcctgcttag ccactgccgc
    18181 gatttgctcg gcagccaaat ccagaccgcc tcacgcagcc aggcactatt tgaagctatt
    18241 cgcgattata tcgacgaacg ctatgcctcc tcgcttaccc gcgaatctgt cgcacaggca
    18301 ttctatattt caccaaacta tctctcgcac ctgtttcaaa aaacgggggc cattggtttt
    18361 aacgaatacc tgaatcacac gcgactggag cacgctaaga cgttattgaa aggctatgac
    18421 ctgaaggtaa aagaagtggc gcacgcctgc ggctttgtcg acagcaacta cttctgccgt
    18481 ctgtttcgta aaaacaccga acgctcgccg tcggaatatc gccgacagta ccacagccaa
    18541 ctgacagaaa aaccgactac tccagaataa gatgggtttg ctgcggcgca gaaagcactt
    18601 tgcgcaccgc gctcattacc cgctgcggct cacgcaggaa tgcgtagatg ctgcattgca
    18661 catagcggca atgttcaaat cgctcggcac cggcaagctc gatatccgta atcaacagag
    18721 caacatccgc ccgacggata tcctcgtccg ctaaacgatt ctccgttccc agcgcgccct
    18781 gagtttcaat gctgacgccc cacttctcta actggcacaa cttttccagc cgttccgccg
    18841 ccatataagt atgcgccacg ccgcttacgc aggcggttac tgccaccagg tatgccattt
    18901 taacctccca cggtaacctg aaatccggcc cgttctgcca tttcgcgcat cgttgccaca
    18961 tcggctgatg acggcgcagc aacatccttc atcgaccatg ttttccccag caggcggtat
    19021 ttcggctcgc cgtactgatg aaacggcaac agatggatct gcctgatatt cagcgggatc
    19081 agcacgtcca gcgcctgctg catattctcc cggctgagtg tgaaaccggg gatcagcggc
    19141 aaacgcggga tcacgttgac accctcactc accagcaaac gcaggttctc cagtacgcgt
    19201 ggcaggttca tcttcaccac atcccgcgcc tgagtcgcgt ccataatttt taaatcgaac
    19261 aacacttcat cgcacaattt cgccagcggt aacagcttgc tggctggtgc gtctccggca
    19321 gtttcaatgg cacatgagac accccacagc cgcagtcgct gtaaaaaacg ggtagcaaac
    19381 tccgcctgca ttaacacttc gccgccagaa agcgtgacgc cgccgccgga cgtgcgaaaa
    19441 aaaatgtcat ctttcatcac ttcccgttcc agagcgtcaa ggctgatatc gcgaccaatc
    19501 cgttcaaacg ccccggaggg gcattcatcc gcatcacgca aacatttcgc acagtgcaga
    19561 catttcgcct ctctgcgtac cgtctggatt ttgccggaga tcgactccgg attagcacac
    19621 cacgggcaaa gatgcggaca gcctttaaaa aagaccaccg tacgaatgcc ctcgccgtca
    19681 ttcagtgaat aacgctgaat gttgaaaatg cgcgccacat cattgaggcg cgtttccaca
    19741 acgttacagc tgatgcgctg tccggcggat gatgtcatcc tggatctcct tcgacagttc
    19801 gacaaagaag gcgctgtatc cggcaacgcg caccaccagc ccggcataat cttgtgggcg
    19861 ctgttgcgct tcccacaacg tgtcggcgtt caccacgtta aactgaatat gctgcaactt
    19921 aagctgggta aacgcccgta agaagtcggc cagtttgcgt aatcccgctt caccttccag
    19981 ggtcgccgga gtgaatttca cgttcagcaa cgtaccgtta gacagcagcg tattatcgag
    20041 cttactgact gacttcagta ccgctgttgg cccttgttcg tcctggccca gcataggtga
    20101 caagccgccg tctgccagct gttctccggc aaaacgaccg tctggcgtcg cgccaaccac
    20161 cgatcccaac gggacgtgag cagaaacggt atacgatccc ggcgtgaagt aaccaccgcg
    20221 cgggttctgg tatttttcca cttctttgca gtagtggcgc aacagttcgg cgctaatgtt
    20281 atccacctcg tcgatatcgt taccatattt ctcaaagcgg ttaattaagc gagcgcggac
    20341 tttttcgcct tctggcgttg caaagttggc ttttaatacc gacagcaatt cgtcaaaact
    20401 taaacgctgt tgctcaaaaa ccatcccttt caacgcatgg agagaatcgc tcaggttggc
    20461 gataccgatc ccctgtacgc cggagaagtt ataacgcgcg ccgccatcgg taatatcgcg
    20521 acctttttcc agacaatcgc tgataaaaga cgagagcagc ggtacaggtg cccagtcgcg
    20581 atggccgata tcacaaatat tgctgccctc aaccatcagg gtgatgtagt ggctgatctt
    20641 ggcgcggatc tgctccagta aaccttcata agtcagcgca gcattgcctt cattttcatg
    20701 caggcagatt tccatcactt tcagcagatt aaacatcgcg atgtcatgca agccgtaggt
    20761 tctgccggga atagataatt ccacacagcc cactacggaa tagtcgcgcg catcttccag
    20821 cgaaacgcca cggttgagga acgctggtac caccacttca tcgttaaaga tttgcggaat
    20881 accggtgccg aggcgaatgg tttcggcggt tttcatcagg aatggcgtgt cgatcaaggc
    20941 gttagtgcgc acgccgaggt tcggttgcgg taattgcacg ctttgatagg cgtcaaggca
    21001 aaggaacgaa agcacgttca ccgcgctacg tccgttctcg gttaacccgc cgagcagtgc
    21061 ggtatagccg gtcgggaaac ctgcgaaata acgcgcgctg ctggtggagc gcaacagcac
    21121 gatgtcgttg catttcaccc ataaagattc gagcagttct ttcaggaacg ccggatcttc
    21181 gccctgggtt aaagatgcct gatagaacgg caacatatac tggtcgaagc gccctaaaga
    21241 cagcgaactg gcgttggatt cgtattgcag aatgatgttc atgtaccaga ataactggca
    21301 cgcctgccag aacgtctgcg gcttatgttc ggcgttatgg cgagagatct ccgcaatagt
    21361 cagcagttct tcgcgacgct gggcatctgt gcagtttgcc gccatcgttt ccgccagtac
    21421 agcgtaacgc aaaatgtgtt tctgcgaggc ttccagcagt aacagcgctg cctgataaaa
    21481 gtgattctcc ggctgttgct gacaatgttg ctgcatctgt gccaccaact cccccagccc
    21541 gtgattcaac aggcgtgggt aatcaataat aatgtgcccc tggcctttat ccgtctggtt
    21601 aatgctgaaa atctgcgtgc tggtcgcggc ttttacttca tccgtcatct gcccgttgat
    21661 gaaatctttc atcgaacgtt tttcccagta cgggaacaac tcttcacgat agatacgttt
    21721 atcttcttcg ctgatagcaa agcggtcctg cggacgcgtc gggaattgat ccagctcttt
    21781 cagcagccag taagggtcca tttccggcga cataatcccg gcgcgcggtt ttacggtgcg
    21841 gttaccggca atcagttctt catcacgaat cgaaatttca acatgttcaa ggatatacgc
    21901 tgttgctttc gcccggcgca atatcaccgg ttcgccttcg gtttgccgat ggctggcggt
    21961 ataaagcagc gcccgctcca gcgagatttc acgggtattg gcaaacagtg cagttttgag
    22021 gcgagagata cgattcgtca tgaggaactc ctgtgggaaa gcgaacccgc ccttgcgagc
    22081 gggagaagaa cttaagcggt ttgcgccagg tgagcttcaa ttttgctcat aatggcgtca
    22141 gcacgtttta ctgcatcgct gatgttgacg cgcacgatgg tcttgccagc aaaacgctct
    22201 tcaaacttga tgccgatatc tttggtcaga atcaccatat cagcgctcgc cacatcttcc
    22261 gcagtcagtt cattttccag accaattgac ccctgggttt ctactttcac ttcccagcct
    22321 ttcgctttcg cggcactttc cagcgcctct gctgccatat aggtatgggc aacacctgaa
    22381 ggacatgcgg ttactgcaat aattttcgtc atggctcgtt cctcaattaa ttaatttcaa
    22441 aatccaaatc caggtcgtct tctttttcat cagtcgaaga cccattttta cgcgccagac
    22501 ttttcagcac gttcacacaa acagccgtca ccactgctcc gacagccact gctgcgatat
    22561 aacccagctt gccttcaacc accggcagca caatcagacc accccagcct gcatagcact
    22621 gcgcaccgac cagcgccgca gttactgcac cacaaactga accgaccatg atggaaggaa
    22681 tgacacgcag cggatcggca gcggcgaaag gaatcgcccc ttcagtaacg ccaacacagc
    22741 ccatcaccag tgccgctttg ccggtttcgc gctcttctgc ggagaaattt ttacgaccaa
    22801 tcagcgtcgc cagccccatc cccagcggcg ggatacaaat acccacggca gcgatgacca
    22861 ccacggtata aacaccctga gcaacgcaaa tcagcatgaa ggcataggct actttgttaa
    22921 ccggaccgcc catatcgaac gccagcatca gacccatgat caccgccagc ataacaatgc
    22981 tgccctgctg catcccctga agccactgag tcaggctgtt ggtcaacgcc cctacaggct
    23041 cgcccaagcc ccacatcatg atgcctgcgg taatcagtgt gccgacgata ggaataatga
    23101 agataggcat caccgagcgc agaactttat gcaccggaat tttcttcagg taatgcacca
    23161 cgatgccgcc gataatcccg gcgatcagtg caccaaagaa gcccgcacca aagctgttac
    23221 caacccaggc accgatagcg cacggagcca gcgcagaacg ctctgcaatg gagtaaccaa
    23281 tgtaagcggc gaggaaaggc accatcagcg tcaaacctgc aacgccgata tcaaacagtt
    23341 ttttcaaatt cggatcggct acggcatccg gcactgcgcc tttgccatac aacatgacgg
    23401 aaaccgccag caaaataccg cccgatacca cgaagggaat catgtgtgaa acgcccgtca
    23461 ttaaatgctg acgggtattt ttcaggatct gcaccaactc attcataagc tgctcccggg
    23521 cttgcgaaag cccatgtcca tatttttgat ggaggaaaca ataggcaatc gcaggcggag
    23581 caaacagtgg ataaaaaggc catttactgg acttttgtaa tatcagtaca aaaatgcgat
    23641 ccgcctcata acttgcgata aaccggaagc atgccgcaaa acggcgtctt gtgagacgcg
    23701 tcgcaaattg acggttacat tacatttgtc cagcttttgg cagatttgtc acatggcagg
    23761 ccgttgccgt gcgctcttat cctgtaaaca gaaaaccgta ccgggagaga ggcaatggcc
    23821 ctgattgtgg aatttatttg tgagctacct aacggcgtac atgcgcgtcc ggcaagccac
    23881 gttgaaacgc tgtgtaatac tttttcatca caaattgagt ggcataacct gcgcactgac
    23941 cgcaagggca acgccaaaag cgcccttgcg ctgattggca ccgatacgct ggcgggcgat
    24001 aactgccagt tactgatttc cggggccgac gaacaggaag cgcaccagcg tttaagccaa
    24061 tggctgcgcg atgaattccc ccactgcgat gcgccgctgg cggaagttaa atctgacgaa
    24121 ctggaaccac tgccggtttc actgaccaat ctgaatccgc aaattatccg cgcccgcacc
    24181 gtgtgcagcg gtagtgcagg cggcattctg acgccgatct cttctttaga tctcaatgcg
    24241 ctgagtaatc ttcccgcagc caaagacgtt gacgccgagc aatccgcact ggaaaatggc
    24301 ctgacgctgg tactgaaaaa cattgagttt cgtctgctgg atagcgacgg tgctaccagc
    24361 gcgatactgg aagctcaccg atccctggct ggcgatacat ctttacgtca gcacttactg
    24421 acaggcgtca gcgagggatt aagctgtgcc gaagcgattg tcgccagtgc gaatcacttt
    24481 tgcgaagagt tcgcccgttc cagcagcagc tacctgcaag aacgtgccct ggacgtacgc
    24541 gacgtctgct tccagttact ccagcaaatc tacggtgagc aacgcttccc ggcaccgggc
    24601 aaactgacgc agcccgccat ttgtatggct gatgaactga ctcccagcca gttcctcgaa
    24661 ctggataaaa atcacctcaa aggtctgtta ctgaaaagtg gcggcaccac ctcacatacc
    24721 gtaattcttg cccgttcgtt caacattccg acactggttg gtgtagatat tgatgccctt
    24781 actccgtggc agcatcaaac gatttatatc gacggcaacg ccggggcgat tgtggttgag
    24841 ccaggggaag ccgtggcccg ttattatcag caagaagccc gcgtacagga cgcccttcgt
    24901 gagcaacagc gtgtctggct gacccaacaa gcccgtaccg ctgacggtat ccgcattgaa
    24961 attgccgcta acatcgctca ctccgtggaa gcgcaggccg cattcggcaa tggtgcggaa
    25021 ggcgttggtt tgttccgcac tgaaatgctc tatatggatc gcaccagtgc accgggcgaa
    25081 agcgagctgt acaacatttt ttgtcaggcg ctggagtctg ccaacggacg cagcattatt
    25141 gtgcgcacta tggacattgg cggcgacaaa cccgttgatt atctgaacat tccggcagaa
    25201 gctaacccgt tcctcggtta tcgcgctgtg cgtatttatg aagaatacgc ctcgctgttc
    25261 accacacaac tacggtcgat cctgcgcgcc tccgctcacg gcagcctgaa aatcatgatc
    25321 ccgatgatct cctcaatgga agagatctta tgggtgaaag aaaaactggc ggaagccaaa
    25381 cagcaactgc gtaacgaaca cattccgttt gatgagaaga tccagcttgg cattatgctg
    25441 gaagtaccgt cggtgatgtt catcatcgat caatgctgcg aagagattga tttctttagt
    25501 attggtagta atgacctgac gcaatatctg ctggcagtgg atcgcgataa cgctaaggtt
    25561 actcgtcact acaacagcct gaatccggca ttcttgcggg cgctcgatta cgccgtgcag
    25621 gcggtgcatc gccagggcaa atggattggt ctgtgcggtg agctgggagc gaaaggttcc
    25681 gtgctgccgt tgctggtcgg cttagggctg gatgaactca gcatgagcgc accatcaatt
    25741 ccggcggcga aagcgcggat gacgcaactt gatagccgtg agtgccgcaa gttgctcaac
    25801 caggcaatgg cctgccgtac ttcgctggaa gtggaacacc tgctggcgca attccgcatg
    25861 acccaacagg acgcaccgct ggtcaccgcc gagtgcatca cgctggaaag tgactggcgc
    25921 agcaaagaag aagtgctcaa aggcatgac
//
LOCUS       DADLTD010000035        25804 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLTD010000035 DADLTD010000000
VERSION     DADLTD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.035"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(82..2301)
                     /locus_tag="JN701_001169"
     CDS             complement(82..2301)
                     /locus_tag="JN701_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY7363684.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="JN701_001170"
     CDS             complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="JN701_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY7363685.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="JN701_001171"
                     /gene_synonym="parF"
     CDS             complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="JN701_001171"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY7363686.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4869..7127)
                     /gene="parC"
                     /locus_tag="JN701_001172"
     CDS             complement(4869..7127)
                     /gene="parC"
                     /locus_tag="JN701_001172"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY7363687.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7673..8155)
                     /locus_tag="JN701_001173"
     CDS             complement(7673..8155)
                     /locus_tag="JN701_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7363688.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="JN701_001174"
     CDS             complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="JN701_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY7363689.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8752..9411
                     /gene="qseB"
                     /locus_tag="JN701_001175"
     CDS             8752..9411
                     /gene="qseB"
                     /locus_tag="JN701_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY7363690.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9408..10757
                     /gene="qseC"
                     /locus_tag="JN701_001176"
     CDS             9408..10757
                     /gene="qseC"
                     /locus_tag="JN701_001176"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY7363691.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10867..11448
                     /gene="mdaB"
                     /locus_tag="JN701_001177"
     CDS             10867..11448
                     /gene="mdaB"
                     /locus_tag="JN701_001177"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY7363692.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYDSDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11479..11793
                     /locus_tag="JN701_001178"
     CDS             11479..11793
                     /locus_tag="JN701_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7363693.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11841..13733)
                     /gene="parE"
                     /locus_tag="JN701_001179"
     CDS             complement(11841..13733)
                     /gene="parE"
                     /locus_tag="JN701_001179"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY7363694.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="JN701_001180"
     CDS             complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="JN701_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY7363695.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="JN701_001181"
     CDS             complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="JN701_001181"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY7363696.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15195..15617)
                     /locus_tag="JN701_001182"
     CDS             complement(15195..15617)
                     /locus_tag="JN701_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY7363697.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETISYQVANAQ
                     YRLTTVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="JN701_001183"
     CDS             complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="JN701_001183"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY7363698.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16452..17933
                     /gene="tolC"
                     /locus_tag="JN701_001184"
     CDS             16452..17933
                     /gene="tolC"
                     /locus_tag="JN701_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY7363699.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            18081..18752
                     /locus_tag="JN701_001185"
     CDS             18081..18752
                     /locus_tag="JN701_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY7363700.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18758..19918
                     /locus_tag="JN701_001186"
     CDS             18758..19918
                     /locus_tag="JN701_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7363701.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19956..20771)
                     /gene="ygiD"
                     /locus_tag="JN701_001187"
     CDS             complement(19956..20771)
                     /gene="ygiD"
                     /locus_tag="JN701_001187"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY7363702.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLV
                     ELLAPVPVTLDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20887..21648
                     /gene="zupT"
                     /locus_tag="JN701_001188"
     CDS             20887..21648
                     /gene="zupT"
                     /locus_tag="JN701_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY7363703.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21706..21876)
                     /locus_tag="JN701_001189"
     CDS             complement(21706..21876)
                     /locus_tag="JN701_001189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363704.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="JN701_001190"
     CDS             complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="JN701_001190"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY7363705.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22893..23041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23103..23453
                     /gene="ubiK"
                     /locus_tag="JN701_001191"
     CDS             23103..23453
                     /gene="ubiK"
                     /locus_tag="JN701_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY7363706.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTE
                     IKKQPDPETLPPTL"
     gene            23737..24287
                     /locus_tag="JN701_001192"
                     /pseudo
     CDS             23737..24287
                     /locus_tag="JN701_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
     gene            24347..>25804
                     /locus_tag="JN701_001193"
     CDS             24347..>25804
                     /locus_tag="JN701_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7363707.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLHGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT         6458 a         6800 c         6374 g         6172 t
ORIGIN      
        1 tcggcatata aataaatgcc taatgagata gattctgatg taatatcccc ggtagcaatt
       61 ctgctaccgg gaacagcgaa tttaacgtcc aaccgccgct ttaggccgtt tcttcgcacc
      121 agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag gcgtctgacg
      181 ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt tctggcgacg
      241 agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac ggctaccaat
      301 cagatgcttt ttacccatcg cttccagcgc ctggcggatt aacggccagt ttgccggatc
      361 gtggtaacgc aacaacgctt tatgcaaacg acgctgcttg tcgcccttcg gtacgaagac
      421 gtcttcactc ttataaccaa tcttcgccag cggttttttc ccggtgtaat acatggtggt
      481 tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga aacgatgctt
      541 tttcagccac agcgccagat tcaccatatc ttcatcacgc gtaccggggt gcgcggagat
      601 gaaatagggg atcagatact gctctttacc tgcctgtttt gagtaagtat cgaacagctc
      661 tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgataacg gcccttcttc
      721 ggtatgttcc ggggcaatct tcagataacc gccgacgtga tgggtcgcca gttctttgat
      781 atagcgcgga tcttccacgg cgatgtcata acgcacacca gaggcaatca ggatcttttt
      841 aatgcctttc agatcacgcg cacggcgata gaggttgatc gttggttcgt ggttcgtgtc
      901 catgtgcgga caaatatccg gataaacgca cgacaaacgg cggcaggttt gctcagcgcg
      961 tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat cggaaattac
     1021 gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga tcgaatcttc
     1081 ggagcggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag agcagccgcc
     1141 aaagcagcca cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg gaatacggac
     1201 attgccatag gccggatgta gcacgcgctt gtatggcagc gcaaaaacgc tgtccatctc
     1261 ttcggtagaa agcggaatag caggcgggtt gatccacaca tagcggtcgc cgtgtttttg
     1321 catcaatgcg cgggcacagc ctgggttggt ttcgtggtgc agaatacgcg aagcatgggc
     1381 gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt aggttttttc
     1441 ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct ttttcggtgc
     1501 caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga ttgggtcgat
     1561 ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca gggcctcttt
     1621 cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc ccatcgccag
     1681 acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca tgtcggcttt
     1741 cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat gtgcggtacg
     1801 acgcagacta gcctcaatac cgccgaggat caccggtaca tctttccacg cctctttaca
     1861 acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt tatccggcgt
     1921 gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca tcgaatccat
     1981 gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcataa agtcgtcttt
     2041 gctgctccag tccggctggg cgatgatccc gacgcgaaag ccctgcgctt ccagcatacg
     2101 accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag taaccaaaat
     2161 gatgtcgcag ctatcccagc caagttgatc catctcttca ctagacatcg gcaaaaacgg
     2221 tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc gatccggttg
     2281 gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat caaaggccgg
     2341 ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc ctcctcgggc
     2401 gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc acggtccgcc
     2461 atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg ctgaccgaaa
     2521 taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc tctgtcttcc
     2581 gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc aatatggaac
     2641 gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg cgcggtgaca
     2701 tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc cagactgata
     2761 tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg catcggaaga
     2821 ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag ggtagaaacc
     2881 agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc cgcttcgcca
     2941 caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg gcgctcgccc
     3001 ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg atcgccagaa
     3061 atcacatgta acgggcgact atcgctcatc tgtagttgat agcgacgaga gttcgacgcg
     3121 ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct ttgtacaccg
     3181 ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta ttctggcgta
     3241 ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac accataatgg
     3301 ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc ggcaaggccg
     3361 ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag agtagctgcg
     3421 ttctggcgaa tgggcagtac gggtgcccag tcagcgtttg gcgacatcat ccgtgccgga
     3481 ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac attttctgtc
     3541 aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat agtcggcccc
     3601 aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa tgaccagtgc
     3661 gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag cggcggaacg
     3721 ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc ggcacaaagt
     3781 gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc tccatgtaaa
     3841 actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga ttgccgactt
     3901 aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg attttttgtt
     3961 ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca tactgactga
     4021 cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga ttaagattaa
     4081 tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc gcaattgccg
     4141 cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg gttccttccg
     4201 ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc gcaatggtgc
     4261 cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac cagtacaact
     4321 gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc ggcggttgca
     4381 caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata tagatagcat
     4441 tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg aacagcggcg
     4501 caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc gggctgaaaa
     4561 ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta ataataagac
     4621 gaaagatata tagcataaca acctctgaag gtctgagaga ctggcattgt acgtcacctg
     4681 aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt taatcgcaac
     4741 gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg aatacgctgc
     4801 cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa caggccctcc
     4861 ccggcatatt actcttcgct atcaccgctg ctggcacggc gaggagagtc gatctcaaca
     4921 cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc gccagtgact
     4981 ttctgtaact cttccgggcg cagtttaatt ttgcgtttcc caacatgaat ggtcagcgtg
     5041 ctttgcggcg gcagaacgta caattgcgcc agaccatctt ctccacgcgc ggcttctgcc
     5101 gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc
     5161 gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca
     5221 atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc cttacctgca
     5281 cggttacgcg ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc
     5341 atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc cccaggcggc
     5401 aacgttaatt tgccggtgag tggttcgccc tgaccacgcg ccgacggcag cgtaatcggg
     5461 tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct
     5521 ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca
     5581 tggcctttag cgctgcgtac ccagcccatc tgcgacagca caatggtgac aggttcagac
     5641 ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga
     5701 cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt
     5761 ttacgctcgg aaaccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc
     5821 tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc
     5881 gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct
     5941 tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa acgccaccag caaaccttcc
     6001 aggatatgca ggcgcttgag gactttctcc agacgatagt tcagtcggcg gcgcacggta
     6061 tcgcgacgga acaccagcca ttcggagagg atttccagca ggtttttcac cgccggacga
     6121 ccatccagac cgatcatatt gaggttaatg cgatagctct tttccagatc ggtggtagcg
     6181 aagaggtggt tcatcacctg atccatatcc acgcggttgg aacgcggcac aatcaccaga
     6241 cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt caaccatcgg cagctttttg
     6301 ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg atgcggcaat
     6361 gcgctgataa ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg cactgaacca
     6421 cgtccgttct cgtagatttt acggatctcg gcgcgcgaag tgataatttc cgcttcagtc
     6481 ggataatccg gcccctgcac gatatccagc agctgatcga gcgtggtttt cggctggtcg
     6541 attaatgcga ttgccgcctg agctacttca cgcaggttat gcggtggaat atcggtcgcc
     6601 atgccgacgg caataccggt ggtgccgtta agcaaaatgt ttggcagacg ggcaggcagc
     6661 attttcggct cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt cccctgcccc
     6721 agctcgctca atagcagctc ggaatatttc gacaaccggg attcggtgta acgcattgcc
     6781 gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct gaccatcaac cagcggataa
     6841 cggtaagaga acggctgcgc catcaggacc atcgcttcat aacaggcgct atcgccgtgc
     6901 ggatggtatt tacccagtac gtcaccgacg gtacgggccg attttttaaa tttggcgctg
     6961 gcattcaggc ccagttcaga catcgcatac acaatgcggc gctgaacagg tttaagacca
     7021 tcgccaataa acggcaacgc acggtccatg atcacgtaca tggagtagtt taagtaggcg
     7081 ttttccgtaa attcatgtag cgcaaggcgc tctgccatat cgctcattaa ttctgattcc
     7141 tcaacttatt cgccagcctt caaaggggca atattgccgg agatactaca ttatctgccg
     7201 cgtcgagtca caggagatca gcgtgaatag cgccgccgga cgtgctccga cggcgataaa
     7261 ggcatcaatg tgccttgata tacaactctt tgctgtagac ttgatcggca tagagggcag
     7321 cacaaggttg cgcccatgcc acaccactcg gcatgggggt ccgctccggg cgattcgaga
     7381 agttgaggtt atgcgagtcc agggaagcct aaagcatcaa agtagtcgac tgtaagtata
     7441 cggttcagcc cagtttagct gtgtctccac cagtgccccg cgcctcctgc cataagtgcg
     7501 gcttgctcat ggataccccc gagacggctt acggcgcacc ggataataaa tttcgcgtat
     7561 acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt
     7621 cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcta
     7681 acggcacata aacatccgtt tccagtcgct gttctgtcat accttcggca agattggtgt
     7741 agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa
     7801 tgatgcccca taccgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat
     7861 aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc
     7921 taacgctgcc gcagatatca aagcgaaacg ctttcggtgc ggtggtggca gggtcgtccc
     7981 aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca
     8041 tgataaattt cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg
     8101 ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc
     8161 ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact
     8221 ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttccccctga
     8281 atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg gtcgatatca
     8341 acattgatag taccgctggc atctttaaac acgtagagat cgtcagagat gcgttcaacg
     8401 atattgccgc gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacagtc
     8461 gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca
     8521 gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc acagggccat tactgcgatt
     8581 actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac
     8641 aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttaatgctg tacagagcgc
     8701 gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt
     8761 ttactgatag aagatgacat gctgattggc gacggcatca aaactggcct tagtaaaatg
     8821 ggttttagtg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgca
     8881 ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg
     8941 cgcgaatggc gagagaaagg tcagcgtgag ccggtactga tcctgaccgc gcgtgatgcg
     9001 ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt
     9061 gcgttgatag aagtcgccgc caggctggag gctctgatgc gccgaaccaa cggccaggcc
     9121 agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg
     9181 gctagcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt
     9241 aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa
     9301 gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt
     9361 gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc
     9421 aacgtcttag tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct
     9481 ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg
     9541 acacccaact gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg
     9601 cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg
     9661 cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaatgat ggcgataacg
     9721 gagaagatat tccctatagc tatcaacggg aaggttttgc tgacgggcaa ctggtcggtg
     9781 aagacgatcc ttggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg
     9841 ttggccagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga
     9901 tcccgtggct ggtcgcactg ccgattatgt taatcatcat gatggtacta ctgggtcgtg
     9961 aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct gactcggaaa
    10021 aaccactaaa cgcgactggc gtacccagcg aagtgcgacc actggttgag tcgctaaatc
    10081 aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg acgctttacc tccgatgcag
    10141 ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca aaccgaagtt gcgcagctct
    10201 ctgacgatga tccgcaggca cggaaaaaag cactgctcca attacattcc gggatcgatc
    10261 gcgctactcg tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc
    10321 ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt
    10381 accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgtc cagggcatca
    10441 aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg
    10501 tgcgttacag tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg
    10561 tgagggataa cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct
    10621 atcgcccctc cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa
    10681 tcgccaagct gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg
    10741 cgaaagtaag ctggtaaggg taagactttt gctaaattcg caaaagactt tgcacatttt
    10801 gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt
    10861 taaaaaatga gcaacattct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt
    10921 caactgaatg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat
    10981 gttcgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc
    11041 tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg
    11101 aaaaaataca ttgatgatgt attcaccgaa ggtcacggga cgctgtatga cagcgatggc
    11161 cgtacccgca aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    11221 tatatgcttt ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc
    11281 ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt
    11341 ggcatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    11401 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    11461 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    11521 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    11581 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    11641 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca ccttgaagcg
    11701 catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggtga cgtgctggag
    11761 atgaatattc gtattctgca gccagggatt taatcctgcc ttgtttgccc ggccatcctg
    11821 accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac
    11881 cagttgcggc gatcttccga gcgtttcttc gccagtagca tgtccatcat cgcgtcagta
    11941 cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc
    12001 gtggtttcgc gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc
    12061 ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc
    12121 gcataataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg
    12181 tgaccgtgtt tcaccaacgc gcggaagtgt tttacaaaca aagcgcagag cagcgtggca
    12241 atgtgcagac catcggagtc agcatccgcc aggatacaga tcttgccgta acgaagctgg
    12301 ctcaaatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcgtg cacttcctgc
    12361 gaagccagca cttcgtcgga agagacttcc caggtgttaa ggatcttacc tttcagtggc
    12421 atgatcgcct gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct
    12481 tccacaagaa acagttcggt gcggttaagg tcctgcgcgg tacagtcagc cagtttgcca
    12541 ggcagcgccg gaccgctggt cagcttttta cgcaccactt ttttagccgc acgcatacga
    12601 cgctgggcgc tggaaatcgc catctctgcc agcaactccg ccgcctgaac gttctggttc
    12661 agccacagga taaaggcatc tttcaccacg ccagaaacga atgccgcgca ctgacgcgaa
    12721 gagagacgct ctttggtctg cccggcaaac tgcggatcct gcatttttac tgacagcaca
    12781 taggcgcagc gatcccagat atcttccgcc gacagcttta caccgcgcgg cagaatgttg
    12841 cggtattcac agaactcacg catcgcgtcc agcaggccct gacgcagacc attaacatgg
    12901 gtaccgccct gcatcgttgg gatcaggttg acgtagcttt cggtcagcag ttcaccgcct
    12961 tccggcagcc acagtagcgc ccagtccacc gcttcagtat cgccagcgaa attaccgata
    13021 aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga
    13081 ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca
    13141 acgccggggc acagtactgc tttggctttc agcacatgcg tcaggcgtga aacagaaaaa
    13201 cgcgggctat cgaagaaggt ttcatccggc cagaaatgga cgctggttcc ggtattgcgt
    13261 ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg
    13321 ttataaatct gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc
    13381 accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta
    13441 ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga
    13501 atatccaccg gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaaa
    13561 ataacgtcca cgcgttttgc gtgacccgcc agtgcttcat ccacactgtt atcaatgact
    13621 tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc
    13681 ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa
    13741 gttagtaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt
    13801 gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca
    13861 ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag
    13921 cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttaa gatcgtaaat
    13981 atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata
    14041 gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa
    14101 acattgtgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga
    14161 atcgccgcca tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg
    14221 cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa
    14281 caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga
    14341 catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc
    14401 tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatggta
    14461 tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc
    14521 aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat
    14581 ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt
    14641 tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg
    14701 cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca
    14761 ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata
    14821 aacacgcgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg
    14881 aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg
    14941 aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg
    15001 tcgaattcgt gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg
    15061 tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa
    15121 atcctgactc tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct
    15181 taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc
    15241 aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta
    15301 ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc
    15361 accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg
    15421 ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac agtcgtcagc
    15481 cgatattgtg cgtttgccac ctgatagctt atagtttcgc cgggtgcgtc attgcgcggt
    15541 aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca
    15601 ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttggtggtg cagctgcagc
    15661 cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat
    15721 gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga
    15781 ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg
    15841 ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagccccgcc
    15901 tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc aatcatcccg
    15961 gcaaccatct ccagtagcca gggggtttcg ctagtgtcgt acgcggcaat tcgaatctgc
    16021 tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg
    16081 cgctcaaaaa tttcccgccg cacctcatga ctcatttgcc cgttgaatag actatgacga
    16141 aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct
    16201 acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg
    16261 gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat
    16321 gtgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg
    16381 gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac
    16441 aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca
    16501 gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc
    16561 cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc
    16621 gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc
    16681 gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta actcaatcca
    16741 tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg
    16801 acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca
    16861 acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc
    16921 gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc
    16981 agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc
    17041 ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatccg gaactggcgg
    17101 cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag
    17161 aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg
    17221 agcaaattcg ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg
    17281 ggatttctga cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg
    17341 acgacagcaa tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg
    17401 gcggaatggt taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc
    17461 aactggaaag cgcgcatcgt agcatcgtgc aaaccgtacg ttcctccttc aacaacatta
    17521 atgcatctat cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat
    17581 tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg
    17641 caaccaccac gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga
    17701 ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac
    17761 tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc
    17821 cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc
    17881 aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac
    17941 gacggggctt cggccccgtc tgaacgtaag acaacgtaaa gatacgggtt atctgccgca
    18001 ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc
    18061 actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca
    18121 aaaactggag cgcacgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc
    18181 tggctagctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt
    18241 cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag
    18301 aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg
    18361 aaggtcagtg ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg
    18421 cccagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc
    18481 tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca
    18541 gcccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag
    18601 gccgcaccat gaccgtaccg aagacagcca tggcaccaaa accggcgacc actactaccg
    18661 ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa
    18721 ccggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga
    18781 gcgcccggac tggcgtgaaa aagcccacga atacggtttc aattttcaca ccatgtacgg
    18841 cgagccgtac tggtgtgaag atgcttacta caagttgccc ctcgcccagg ttgaaaagct
    18901 ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc
    18961 cagcgatgag ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgtca
    19021 gtcatggctg actcaccagc catcgcttta ttcgcgtctt gatctggcat gggatggcac
    19081 tggcgaacct aaactgctgg aaaataacgc cgatacacca acgtcactat acgaggcggc
    19141 gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag
    19201 cgaccagttt aacagtctgc aagaaaagct aatcgatcgc tttgtcgagt tgcgtgaaca
    19261 gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac
    19321 cattcagtat ttgcaggact gcgcgacgga agctgaaatc gctaccgagt tcctctacat
    19381 tgacgatatc gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc
    19441 caacctgttc aagctgtatc catgggaatt tatgttgcgt gagatgttct ccaccaagct
    19501 ggaggatgca ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaagc
    19561 gcttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt
    19621 tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga
    19681 aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta
    19741 tggcgaagaa gggatgattg ttcagcaatt ccatccgtta ccgaaattcg gcgacagcta
    19801 tatgctgatt ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga
    19861 ccgtgcattg atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc
    19921 aatgatgccg gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata
    19981 ctcaggctgc ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac
    20041 gcacctaaga catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca
    20101 ccttcatggt cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga
    20161 ttcgctttca catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc
    20221 cacttcactg tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca
    20281 tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg
    20341 tcgatactca actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc
    20401 caggaaccgt ggtcaaagcc cctggcttct ttatccagcg tcaccgggac cggcgctaac
    20461 agctcaacca gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac
    20521 agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact
    20581 cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat
    20641 gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga
    20701 ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact
    20761 aagggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa
    20821 caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag
    20881 ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc
    20941 gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt ttcgctaggt
    21001 tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca
    21061 gctgaaggaa tatcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctac
    21121 tttggcctgg accgcatgtt gccgcatgcg catccgcagg atttaatgca aaaatcggtg
    21181 cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg
    21241 cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga
    21301 tttggcatcg cactggccgt cgcgttgcac aatatccctg aaggtctggc agtggcaggc
    21361 ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga
    21421 ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg
    21481 gtggtcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    21541 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    21601 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    21661 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    21721 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    21781 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    21841 agcaatcaat acaatccagt accaggcgat aaacatcctt tacccttaat atttaacaat
    21901 actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca tatggtcgca
    21961 taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg atattaatga
    22021 aattttctca tagaaagaga agcggaatta acatcttgca tagcagggca aaataaaaac
    22081 cgaagccagc tacaacttcg gtttcttgat taaggcagta attaagcagc ggttttcagc
    22141 tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca
    22201 tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat
    22261 tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc aaatcaatag
    22321 tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa
    22381 cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg
    22441 tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca
    22501 cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc agatcaagtt
    22561 gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca
    22621 gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac
    22681 ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt
    22741 caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaaa
    22801 ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa
    22861 aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac
    22921 cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc
    22981 gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag
    23041 cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt
    23101 ttatgactta cccttatcgc actacaatgg tactcaacac ctatcagtac agggaaacta
    23161 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca agttcacgaa tcaatgccta
    23221 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc
    23281 agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt
    23341 tacggactcg tgagaaactg gcgctgcttg aacaaagaat gagcgaactg gaaaatcgca
    23401 gtactgagat taaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg
    23461 tctttaatgc actgtaaatt atgaattaat cttctaattt acagtgcttt ctattacctt
    23521 atcgcttaat tccattcaga atatcaccac aaaaatgaat gagtgcaatt gaaaatataa
    23581 ttgttatgta tttttttaag ttttacttaa tccatattta gataaactaa tttcataaaa
    23641 cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca gttactaatc
    23701 attcattttc tacctatatt tatataaagg aatattatgt ctgcttttaa aaagtcactt
    23761 cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga aggtcacggc
    23821 attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc aggtgacgaa
    23881 gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga ccagaaatct
    23941 ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg tggaaccact
    24001 tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca gactgacctg
    24061 ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt acgtattctg
    24121 aatagccaga gcggcgaagt gactttaggt actccggtag taattacatt caacaatact
    24181 aacagctatc aggaactgaa cttcaaagtt cgtatggatc cccgggcaaa gatgctaccc
    24241 cgggcaacgt ttacgctcag gctgactata agattgctta cgagtaatca cattaggccg
    24301 ggttcgcccg gccacatcat taacggatta atgataagtg gaacagatgt ataaaaaatt
    24361 aaaattaacc acaataagcg aattgattaa aaatatttat tgttcattat ccgttctcat
    24421 cattggttgt gcgtcagctt atgccgttga attcaacaaa gatttaatcg aagccgaaga
    24481 tcgtgaaaac gttaaccttt cccaatttga aactgatggc caattacccg taggtaaata
    24541 ctcattaaac gctattatta ataataaaag gacgccaatc catctggacc tgcaatgggt
    24601 attaattgat aaccaaactg cagtttgtct gactccagag caattaacat tattaggctt
    24661 tactgatgag attattgaag aggctcagca aaacctgatc gatggttgtt accctatcga
    24721 aaaagaaaaa caaattacaa cttatctcga taaagggaaa atgcaattat ccatatctgc
    24781 acctcaggca tggttaaaat acaaagatgc aaactggacg cctcctgaac tttgggatca
    24841 tggtattgct ggggcatttc ttgactacaa tttatatgcc tctcattatg caccacatca
    24901 gggcgataat tcacaaaata taagttccta tgggcaggct ggggttaatc ttggggcctg
    24961 gcgcctgcgt actgattacc agtacgatca gtcatttaac aatggcaaaa gccaggcgaa
    25021 caacctggat tttccgcgta tttatttgtt tcgcccaatc ccagcaatta atgcaaaact
    25081 aactataggt caatacgata ctgaatcctc tattttcgac tctttccatt tttctggcgt
    25141 ttcgttgaaa agcgatgaga atatgttgcc gcctgatcta cgtggttacg caccgcaaat
    25201 cacgggtgtt gcacaaacga atgcaaaggt cactgtctca cagaacaacc gtattattta
    25261 tcaagaaaat gttcctcctg gcccatttgc tattactaat ttattcaata cattacaggg
    25321 gcaactggac gtcaaggttg aagaagagga cgggcaagtc acgcaatggc aagttgcatc
    25381 taatagtatt ccttatctga cgcgtaaagg gcagattcgc tacaccactg ctatgggtaa
    25441 accgaccagc gttggtggtg attccttaca acaacccttc ttctggactg gtgaattctc
    25501 atggggttgg ctgaacaatg tatccctgca tggtggttca gttttaacaa accgtgatta
    25561 tcaatctctg gctgccggcg ttggctttaa ccttaactca ttgggttcat tatcttttga
    25621 tgtcacacga tctgatactc agttgcataa tcaggataaa gaaacgggtt atagctaccg
    25681 cgctaactat tcaaaacgtt ttgaatctac cggtagccag ctcactttcg ctggttaccg
    25741 tttctctgat aaaaactttg tgacaatgaa tgaatatatc aatgacacta accattacac
    25801 gaat
//
LOCUS       DADLTD010000036        25556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLTD010000036 DADLTD010000000
VERSION     DADLTD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.036"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(37..1335)
                     /gene="eno"
                     /locus_tag="JN701_001194"
     CDS             complement(37..1335)
                     /gene="eno"
                     /locus_tag="JN701_001194"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY7363708.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1423..3060)
                     /gene="pyrG"
                     /locus_tag="JN701_001195"
     CDS             complement(1423..3060)
                     /gene="pyrG"
                     /locus_tag="JN701_001195"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY7363709.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3288..4079)
                     /gene="mazG"
                     /locus_tag="JN701_001196"
     CDS             complement(3288..4079)
                     /gene="mazG"
                     /locus_tag="JN701_001196"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY7363710.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4150..4485)
                     /gene="mazF"
                     /locus_tag="JN701_001197"
     CDS             complement(4150..4485)
                     /gene="mazF"
                     /locus_tag="JN701_001197"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HAY7363711.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4485..4733)
                     /gene="mazE"
                     /locus_tag="JN701_001198"
     CDS             complement(4485..4733)
                     /gene="mazE"
                     /locus_tag="JN701_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HAY7363712.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4811..7045)
                     /locus_tag="JN701_001199"
     CDS             complement(4811..7045)
                     /locus_tag="JN701_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY7363713.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7093..8394)
                     /gene="rlmD"
                     /locus_tag="JN701_001200"
     CDS             complement(7093..8394)
                     /gene="rlmD"
                     /locus_tag="JN701_001200"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY7363714.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8451..11207
                     /gene="barA"
                     /locus_tag="JN701_001201"
     CDS             8451..11207
                     /gene="barA"
                     /locus_tag="JN701_001201"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY7363715.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11439..12779)
                     /gene="gudD"
                     /locus_tag="JN701_001202"
     CDS             complement(11439..12779)
                     /gene="gudD"
                     /locus_tag="JN701_001202"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7363716.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12800..14140)
                     /locus_tag="JN701_001203"
     CDS             complement(12800..14140)
                     /locus_tag="JN701_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7363717.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14142..15512)
                     /locus_tag="JN701_001204"
     CDS             complement(14142..15512)
                     /locus_tag="JN701_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7363718.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15929..16378)
                     /locus_tag="JN701_001205"
     CDS             complement(15929..16378)
                     /locus_tag="JN701_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY7363719.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16396..17178)
                     /gene="truC"
                     /locus_tag="JN701_001206"
     CDS             complement(16396..17178)
                     /gene="truC"
                     /locus_tag="JN701_001206"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY7363720.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17178..17507)
                     /locus_tag="JN701_001207"
     CDS             complement(17178..17507)
                     /locus_tag="JN701_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY7363721.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18129..18674)
                     /gene="syd"
                     /locus_tag="JN701_001208"
     CDS             complement(18129..18674)
                     /gene="syd"
                     /locus_tag="JN701_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY7363722.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18742..19590
                     /gene="queF"
                     /locus_tag="JN701_001209"
     CDS             18742..19590
                     /gene="queF"
                     /locus_tag="JN701_001209"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY7363723.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19702..21066
                     /gene="ppnN"
                     /locus_tag="JN701_001210"
     CDS             19702..21066
                     /gene="ppnN"
                     /locus_tag="JN701_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY7363724.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21623..22912
                     /locus_tag="JN701_001211"
     CDS             21623..22912
                     /locus_tag="JN701_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7363725.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22970..24337
                     /gene="sdaB"
                     /locus_tag="JN701_001212"
     CDS             22970..24337
                     /gene="sdaB"
                     /locus_tag="JN701_001212"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY7363726.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24359..25204
                     /gene="xni"
                     /locus_tag="JN701_001213"
     CDS             24359..25204
                     /gene="xni"
                     /locus_tag="JN701_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY7363727.1"
                     /translation="MRGLFPISHPAVACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHDEMPALRAAFEQRGVPCWSTSGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(25309..25556)
                     /locus_tag="JN701_001214"
                     /pseudo
     CDS             complement(25309..25556)
                     /locus_tag="JN701_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         6180 a         6820 c         6193 g         6363 t
ORIGIN      
        1 cggaggctgg catttttaaa tcagataaag tcagtcttat gcctggcctt tgatctcttt
       61 acgaccgttg tacggtgctt tttcgcccag agcttcttcg atacgaatca gctggttgta
      121 tttagcaaca cggtcagaac ggctcataga accagttttg atctggcctg cagcagtacc
      181 aacagccagg tcagcgatgg tagcgtcttc agtttcgcca gaacggtgag agataactgc
      241 agtgtagcca gcatctttcg ccatcttgat tgcagccaga gtttcggtca gagaaccgat
      301 ctggttgaat ttgatcagga tggagttagc gatacctttt tcgatacctt ctttcaggat
      361 cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt
      421 ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat
      481 cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt
      541 gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc
      601 catcgccaga gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat
      661 aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac
      721 agcagtgttc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc
      781 catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat
      841 atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat
      901 agagtatttg cccggagtac cgttcagttc agcgatgtgc tcgtacagcg gcataccttt
      961 agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa
     1021 tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc
     1081 tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt
     1141 ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg
     1201 ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg
     1261 tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt
     1321 tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac
     1381 aacacgtacc cagggtacgc gttgccgttc taactttttt acttacttcg cctgacgttt
     1441 ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt
     1501 agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat
     1561 gatctcgacc aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat
     1621 ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg
     1681 cgcattgtac agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag
     1741 acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc
     1801 gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attccgtaga
     1861 gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc
     1921 caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat
     1981 gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag
     2041 gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac
     2101 acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag
     2161 ttcaatgtac ttgccgacca taccgatggt gacttcactt accgggttcg cttcttcgaa
     2221 gataacctgt tcccattcgg acagattcgc ttccgggcag tttaagctga atcgtttaca
     2281 aatataatcg tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac
     2341 gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg
     2401 ttcgttcgcc ggaacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat
     2461 ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc
     2521 catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc
     2581 catctggcga atcgcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat
     2641 ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt
     2701 agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg
     2761 cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg
     2821 aatgaaacgc tcgtagtgcc ccaggtccag gtcggtttca gcgccgtctt cagtaacgaa
     2881 cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt acggatccag
     2941 tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc
     3001 aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat
     3061 gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaggacggg aaagcagtat
     3121 acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt
     3181 gaaatcagga gtgagaaaat agcccctttg ggataaatgt ttttgacgca aatcaagcgc
     3241 ttgtcattta aaaaatgaca caaatggcac ttgaccgcgt aattccctta gagatcaatt
     3301 tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt
     3361 tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg
     3421 ttcgcttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg
     3481 gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggtcgacaac agcctgccgc
     3541 gcttcgtaca tcacctcgtc gatctcttcg tagactttat caaccaccgg gccaagcgtc
     3601 gtccagtcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcccg cattaaagcc
     3661 ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg
     3721 gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca
     3781 aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag
     3841 tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg
     3901 cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag
     3961 gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag
     4021 ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacgatcgat ttgattcatt
     4081 gaattgtcct gaaaattgcg ggtctgtcag gtggaaacct gtgaccagaa tagaagtgag
     4141 ttagtaacac tacccaatca gtacgttaat tttggcttta atgagttgta attcctctgg
     4201 ggcaactgtt cctttcttcg ttgctcctct tgcccgccag gcgattcttt ttacctgatc
     4261 agctaacgct acgccatcac gttcctgacc ggataaaaca acttcgaacg gatatccttt
     4321 tgattgcgtt gtacaaggaa cacacagaca catacctgtt ttgttgttgt acatgaaagg
     4381 actcaggaca acagctggac gatgtccagc ttgctcgcta ccttttgtcg ggtcaaaatc
     4441 aacccaaatc agatcgccca tatcgggtac gtatcggctt accattacca gacttcctta
     4501 tctttcggct ctccccagtc gatattctcg tggaggtttt ccggcgtgat gtcgttgacc
     4561 agttcagcaa gcgtaaatac gggctcttta cgcactggct caataattaa tttgccatcc
     4621 accaggtcaa tcttcacttc atcatcaata ttcagattga gcgcctgcat taacgtagcc
     4681 gggatccgca ccgccggtga atttccccaa cgctttacgc tactgtggat catcaccctt
     4741 tcctcaaacc gctatcatat gtagatacag tatatatcaa tctacattgt agatacgagc
     4801 aaatttctgc ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     4861 gtttacccag cacgcggccc agcacctgca ggttgtaaat ctcaatggtc atgtcgatgg
     4921 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagtacgttc accttctcgt
     4981 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5041 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5101 gcgcatgaga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5161 gcccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5221 tgtggtgcat caggttgcca acaccttcga ctaccacgcg accgttatct ttactgcggt
     5281 tttgcggcgt gtagcttttt tgctgaagtt gcttcagcgc ggcggcgtcc tgctcttcgg
     5341 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5401 cgccaccaat cgccgccagc aactcgtcga catcattaaa gttgtaacgc ggcagcagat
     5461 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5521 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5581 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5641 gctgtttctg ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca
     5701 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5761 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     5821 agacatacac ccggtcgtca aagacctggc tgcgtacttc gtcgagcatt tcgccggaat
     5881 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     5941 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6001 actctgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt ttgccacctg
     6061 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacataat
     6121 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6181 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6241 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cggccataca
     6301 cttcagcttt aacgccttca gctttcatct cagcgcgcag atgaccgaca aactcttcga
     6361 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6421 tcgggtgcag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6481 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acgcgttcat
     6541 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacca
     6601 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6661 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6721 cgtgaataag gttaacgaca gacttaccga cgctctcacg cagcacatct tcgctgacta
     6781 cgttggcatc ggccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     6841 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     6901 gcccctgcgt ctgctgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     6961 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7021 tatgtgcact tcttaccgca accattgtcc tctcctttag ggaccagacc tgccgaaatc
     7081 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7141 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7201 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7261 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7321 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7381 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7441 cggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7501 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7561 cgcactcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7621 aaaagttaag cgcaacccgt tagagtcata ccagggcatc tcaccagaga cggtttcgag
     7681 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7741 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7801 ggttgcctgt accagttcaa catgaccaag atggcgcatt gcttgtaagc tgcccaggca
     7861 tgccctgact ttgggcagca atgcttcaag ttggggcact aaaatgggac attgtttaac
     7921 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     7981 taagtagttc agacttaaac gcgcgcgacg gcgatagccc cagggaacat cggcgatcac
     8041 ttcagagact tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8101 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8161 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8221 tttatcttca gtaacagtaa cttccgcgtt ttcctgcggc aataatccgg ggataaatag
     8281 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8341 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8401 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8461 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8521 tgctgagtat atttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8581 caggtgccag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8641 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8701 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8761 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     8821 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     8881 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     8941 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga
     9001 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9061 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9121 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9181 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9241 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9301 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9361 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9421 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9481 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9541 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9601 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9661 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9721 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9781 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
     9841 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
     9901 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
     9961 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10021 ttattatcga agggccatcc acccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10081 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10141 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10201 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10261 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac
    10321 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctaca cgcctgttgc
    10381 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10441 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10501 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcgg
    10561 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10621 tggatggcat tcgggcctgc gaactcatcc accagctccc ccatcagcaa caaacgccgg
    10681 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10741 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10801 acaagcctgg cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    10861 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    10921 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    10981 ttgaggaaca gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11041 atggcagttg cggctatagc ggtgtgccgc gtatgaagaa tctctgccaa ctgatcgaac
    11101 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11161 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11221 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11281 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11341 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11401 aagccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11461 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatt gtcacgcgcg
    11521 caaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11581 acacccagcc ccggtttttc cggcacctga accagaccgc ctttgatctc aaacggttct
    11641 ttggtcaagc gctgattgcc ttcctgccaa atccagtgcg tatcaatcgc ggtgatttta
    11701 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11761 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    11821 tgcatcgtcc agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta
    11881 tggcccatct gccgccagtc ggtggcgatc atattggttg cggtcggcag gccagttgcg
    11941 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12001 tctgcataag ccagcgaccc tttcaggtat ttaccaattt taatcgcttc gttcagcgac
    12061 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12121 atagactcgg cctcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12181 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12241 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggt
    12301 gtggctttgc gatcaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12361 tgaccatcgc ccagcaggga tgccacgttt acccccagat gctgccccag cagatccagc
    12421 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12481 ccgcgcccac cagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12541 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12601 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt gataatcata
    12661 atattacgcg taaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12721 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12781 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    12841 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcca ggcagacgtt
    12901 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    12961 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13021 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13081 ccacatgggt aaacatcgcc agagagatat cgaaatggtt attagaatgg cagccccagg
    13141 tcagccccca gtcgtcgcaa agctgcgcca cacggactgc accggaaagc gtccagaagt
    13201 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13261 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgccga aactccgcca
    13321 taacttcacg accggagaaa ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13381 catcattcag ccctttgcat agagaaatgg cttcatcaag tagccaggca ccgttgggat
    13441 caacggtaat ccgcgcatca gggaagcgtt tcttcaatgc acgaacagtg tcgatttctt
    13501 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13561 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13621 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13681 cgatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13741 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13801 ccgccacggc attaacgtgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    13861 cggctgcctg attaccttta tgcacctgct gaaccacttt attcaggcgc gcaacttcct
    13921 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    13981 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacaatattg cgggtgaaat
    14041 atgcgttatg tgccccacca atattaagca acatgctatc gtgcccggcg accggaatga
    14101 ctttcatatc agtaataaca ggactggatt gtgtcgtcat cttattgccc cgcgacaggt
    14161 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattaag
    14221 gcatgaacac caacataaat cagcgcccca ttaaaggagc cagtcgtgcc aacgatataa
    14281 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14341 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14401 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14461 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14521 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataatccc acccagcaca
    14581 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaattgac
    14641 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14701 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagttgtttg
    14761 atctgccccc acttcacgct gaacggcact ttaactttgg tgttttgctg atccatattg
    14821 atcagcgcgc cacccgcggc gatgtactcc agctctttct gatttacccc cggatgttga
    14881 tttggctcgt ggatgacttt caaccagata aagctgatga caatccccag accgcccata
    14941 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15001 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15061 gccggaaacc aagccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15121 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15181 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15241 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15301 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15361 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15421 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15481 cttaaagaac tcgttgttgc actcctgaaa attcgcgtta gccacgctaa ctctggactg
    15541 cgacatagcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15601 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15661 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15721 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggttaaaac
    15781 gaagaaataa agaagagtat gaaatggatc gcttgactcc aggcaaacgc cagtaaaaat
    15841 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    15901 aaacgacatt taccgctcgc tgaacatatc aggacaacag cgtgccccac tgttcgaccc
    15961 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16021 caactcgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaga
    16081 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16141 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16201 cggtcgtgga cgtaaccacc agaacatact tatcctgata aggcagccag tcgcttaatt
    16261 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16321 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16381 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16441 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16501 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16561 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16621 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16681 tcggcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16741 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16801 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    16861 ttccaccagt ggataatcca gcaccgcttc ttccatcaac cagccgcgca caatcgcatg
    16921 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    16981 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17041 atgctgtcct atctggttac gcacggtttg catgaccact actttctcgt cgcgatccag
    17101 ccagctacgg tgaaccagcc agccggaggg tttatttacc gaaaccagcc attcatcctg
    17161 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17221 tcagccagaa taagcgcgtg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17281 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17341 atcagcaccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17401 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17461 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17521 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagcg
    17581 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17641 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17701 ccccgatgtg atacttcatc ctgaagtgtc cttggccttc ctgaccagcc gaatcatcct
    17761 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    17821 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    17881 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    17941 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18001 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18061 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18121 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18181 tgcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18241 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18301 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18361 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18421 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18481 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18541 gtagttgaaa taatgcacgg tgaaggaaca ccgtacagtt cctcacttaa cggccagctt
    18601 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18661 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18721 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18781 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    18841 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    18901 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    18961 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19021 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19081 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19141 cttgatgaac tggaaggcca gccgataggt cattttaatg gcacttgcat tgatgaccag
    19201 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc cacctgtggt
    19261 gaaaaagtag tggaagagac gcttgtcagc cacctgctga aatcaaactg cctgatcacc
    19321 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19381 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19441 gaacgcatct ttaatgacct gttacgcttc tgccagccag aaaaattgag cgtttacgca
    19501 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19561 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19621 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19681 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19741 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19801 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    19861 ttgctgtctc gttttgaaaa tttcgatatt aacgtcttgc gccgtgaacg cggcgtaaag
    19921 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    19981 caggccaact tgttcgcggt cctgcgtgac attctcttcg tttacgggca aatccataac
    20041 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20101 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20161 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cggaaaccag
    20221 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc cgggagcgat ggaagcgccg
    20281 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20341 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cgctggtcaa cgaattgatc
    20401 atcatgccag atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggtatcatt
    20461 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20521 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20581 gactacttcc gcgtactgga cgagtttgtc gtgcatacgc tgggtgaaaa cgcgcgccgc
    20641 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20701 ccgctggtga aagaaaatcg ccgcgacaca ggcgatgcct acagctttaa ctggtcaatg
    20761 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    20821 aagctttacc cggatcaacc cgttgaagtg ctagctgccg acctgcgccg tgcgttctcc
    20881 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    20941 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21001 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21061 acgtaatctc ccctcccccg gtgacgtttg ttgccgggtt ttctcgtttt tggtcactta
    21121 ctcatcaact catttcattt gttatatgaa tgtttcttat caccctcacg cgacaaatat
    21181 catcacagtt aatatgtcat acaatttatg ttgcaacgca aacgtttccc tattttcata
    21241 aaccgtattt tatcgctatg aaaaagaaat tatcgccatg attaactaaa agtattgatt
    21301 ttttcagttc aacctacata tattgcgcgc cctggaagaa gtcagatgtc gtttaatggg
    21361 caaatattgc ccttaaattc tcttttactt ttgatttaca gagtaaagcg ttgggataat
    21421 ctatcttcca agtagattat tgtatttgag atcaagatca ctgatagata cataacttgt
    21481 gtgtatcttt ccgccctcaa attattacgg cggtaaatga ttaagccatc gccgatagac
    21541 agatttcatt tttacggtca ggcaccttcc cgggctgaac tggctaaaag ctgaattatt
    21601 tgcattcctc caggagaaat agatggaaac gactcaaacc agcacgattg cgtcgaaaga
    21661 ctctcgtagt gcctggcgca agacagacac catgtggatg ctgggccttt acggcacggc
    21721 aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt gttggcggta tgatcccgct
    21781 gatcatcatg gctatccttg cgttcccgat gacgtttttt gctcaccgcg gcctgactcg
    21841 cttcgtactg tctggtaaaa acccgggcga agacatcacc gaggttgtag aagaacactt
    21901 tggtattggc gcaggtaaac tgattaccct gctctacttc ttcgctatct acccgatcct
    21961 gctggtttat agcgtggcaa tcaccaatac agttgaaagc ttcatgtctc accagctggg
    22021 tatgacgcca ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta tgatgaccat
    22081 cgttcgcttc ggtgagcaga tgatcgttaa agcgatgagt attctggtgt tcccgtttgt
    22141 tggcgtattg atgctgctgg ctctgtacct gatcccgcag tggaacggcg cagcactgga
    22201 aacgctgtct ctggacactg catctgcaac cggaaacggt ctgtggatga ccctgtggct
    22261 ggcaattccg gtaatggtgt tctcgttcaa ccactctccg atcatctctt ctttcgccgt
    22321 tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa tgctcgaaga tcctggcatt
    22381 cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc ttcagctgtg tactgagcct
    22441 gactccggca gacctggctg cggctaaaga gcagaacatc tcgattctgt cttacctggc
    22501 taaccacttt aacgcaccgg ttatcgcgtg gatggctccg attatcgcga ttatcgctat
    22561 caccaaatcc ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg gtatggtgat
    22621 taaatctctg cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc gtatcactgc
    22681 gctgttcatg ctggtaacga cctggattgt tgccaccctg aacccgagca tcctgggtat
    22741 gattgaaacc ctgggcggtc caatcatcgc gatgatcctg ttcctgatgc cgatgtacgc
    22801 aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg tattcgttgt
    22861 cgtgatgggt ctgattgcaa tctccgcaat cttctactct ctgttcagct aagtcctttc
    22921 gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat gtatttccta tgattagcgt
    22981 attcgatatt ttcaaaatcg gcattggccc ttccagttct cataccgttg gaccaatgaa
    23041 agcgggtaaa caatttaccg acgatctgat tgcccgtaac ctgcttaaag acgtgacccg
    23101 cgtggtggtt gacgtgtacg gctcgctctc tctgaccggt aaaggccacc acactgatat
    23161 cgccattatt atgggcctgg cgggtaacct gccggatacc gtggatatcg attccatccc
    23221 tggttttatt caggatgtga atactcatgg tcgcctgatg ctggcaaacg gtcagcatga
    23281 agtggagttc ccggttgatc agtgcatgaa cttccacgcc gacaaccttt ctctgcatga
    23341 aaacggtatg cgcattaccg cgctggcggg cgataaagtc gtttacagcc agacttacta
    23401 ctctattggc ggtggcttta tcgttgatga agagcatttt ggccagcagg atagcgcacc
    23461 ggttgaagtt ccttatccgt acagttcagc agccgatctg caaaaacatt gtcaggaaac
    23521 cgggctgtca ctctctggcc tgatgatgaa aaacgagcta gcgctgcaca gcaaagaaga
    23581 gctggaacag cacctggcga acgtatggga agtcatgcgt ggcggtattg agcgcggtat
    23641 ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca cgccgtgctg cggcactacg
    23701 ccggatgctg gtcagccagg ataaaaccac cactgacccg atggcggttg ttgactggat
    23761 caacatgttt gcactggcag tgaacgaaga gaacgctgct ggcggacgcg tggtgactgc
    23821 gccgactaac ggtgcgtgcg ggattatccc ggcagtgctg gcgtactacg acaagtttat
    23881 ccgcgaagtg aacgctaact cactggctcg ttacctgctg gtagccagtg ccattggttc
    23941 tctttataag atgaacgcgt caatttctgg tgctgaagtc ggctgccagg gtgaagttgg
    24001 tgtggcgtgc tcaatggcgg cggctggtct ggcagaactg ttaggtgcaa gcccggcgca
    24061 ggtgtgcatc gcggcggaaa tcgccatgga gcacaacctc ggtctgacgt gtgacccggt
    24121 cgccggacag gtccaggtgc catgcatcga gcgtaacgcc attgcggcag taaaagcggt
    24181 gaacgccgca cgtatggcgc tacgccgtac cagcgagccg cgcgtctgcc tcgataaagt
    24241 tatcgaaacc atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg aaacctctcg
    24301 cggcggcctg gcaatgaaga tcgttgcctg cgattaatcg ctctccagag gcctcgtttt
    24361 gcgaggcctc ttcccgattt ctcatccagc cgtagcctgt tccggcatcg aatgttaccc
    24421 ttatcgcctg atctttaagg gggttatcgt ggctgttcat ttgcttattg tcgatgcact
    24481 gaatcttatt cgtcgcattc atgccgttca gggatcgccc tgtgtcgaaa cctgccagca
    24541 tgcgctcgat cagctcatta tgcacagcca gccaacccac gcggtcgccg tttttgatga
    24601 tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca gattacaaag cgggtcgacc
    24661 gccaatgccg gaagagttgc acgacgagat gcctgcatta cgcgccgcct ttgagcaacg
    24721 cggcgtcccg tgctggtcaa ccagcggcaa cgaagccgat gacttagccg ccacgctggc
    24781 ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt tcgacagata aaggctactg
    24841 tcagttactt tcaccgacat tacgtattcg tgattacttc cagaaacgtt ggctggatgc
    24901 gccatttatc gataaagaat ttggcgttca accgcagcag ttgcccgatt actggggact
    24961 tgcggggatc agcagttcaa aggtaccggg tgttgcggga atcggaccaa aaagcgccac
    25021 gcagctgctg gtcgagtttc agagtctgga agggatatat gagaatctgg atgcggttgc
    25081 cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt gccgcgatat
    25141 tgcccgctta caaaccgatt tgcatatcga cggcaattta cagcaattgc ggttggtacg
    25201 gtaacggcga gccggatgca aacgattatc aacacattga atttaatgca cttatatacc
    25261 ggataagacg cggctagcgt cgtatccggc attatcacat cagcgcattt accaggcggt
    25321 atggtaaagc tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac
    25381 atcatccagt gccgcctgcg ccagtgccgg aatgtcttcc ttgcgtacac caacatcacg
    25441 caaatgtggc ggaataccga catcacggtt gagagcaaac accgcttcaa cagcggcatt
    25501 acgcgcctct tccagccact ttcacgccca taacgcgcgc gatatcgcgg tacttc
//
LOCUS       DADLTD010000037        25242 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLTD010000037 DADLTD010000000
VERSION     DADLTD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.037"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..403)
                     /locus_tag="JN701_001215"
     CDS             complement(<1..403)
                     /locus_tag="JN701_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY7363728.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLP"
     gene            782..1093
                     /gene="rpsJ"
                     /locus_tag="JN701_001216"
     CDS             782..1093
                     /gene="rpsJ"
                     /locus_tag="JN701_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY7363729.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1126..1755
                     /gene="rplC"
                     /locus_tag="JN701_001217"
     CDS             1126..1755
                     /gene="rplC"
                     /locus_tag="JN701_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY7363730.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1766..2371
                     /gene="rplD"
                     /locus_tag="JN701_001218"
     CDS             1766..2371
                     /gene="rplD"
                     /locus_tag="JN701_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY7363731.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2368..2670
                     /gene="rplW"
                     /locus_tag="JN701_001219"
     CDS             2368..2670
                     /gene="rplW"
                     /locus_tag="JN701_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY7363732.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2688..3509
                     /gene="rplB"
                     /locus_tag="JN701_001220"
     CDS             2688..3509
                     /gene="rplB"
                     /locus_tag="JN701_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY7363733.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3526..3804
                     /gene="rpsS"
                     /locus_tag="JN701_001221"
     CDS             3526..3804
                     /gene="rpsS"
                     /locus_tag="JN701_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY7363734.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3819..4151
                     /gene="rplV"
                     /locus_tag="JN701_001222"
     CDS             3819..4151
                     /gene="rplV"
                     /locus_tag="JN701_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY7363735.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4169..4870
                     /gene="rpsC"
                     /locus_tag="JN701_001223"
     CDS             4169..4870
                     /gene="rpsC"
                     /locus_tag="JN701_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY7363736.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4883..5293
                     /gene="rplP"
                     /locus_tag="JN701_001224"
     CDS             4883..5293
                     /gene="rplP"
                     /locus_tag="JN701_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY7363737.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5293..5484
                     /gene="rpmC"
                     /locus_tag="JN701_001225"
     CDS             5293..5484
                     /gene="rpmC"
                     /locus_tag="JN701_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY7363738.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5484..5738
                     /gene="rpsQ"
                     /locus_tag="JN701_001226"
     CDS             5484..5738
                     /gene="rpsQ"
                     /locus_tag="JN701_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY7363739.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5903..6274
                     /gene="rplN"
                     /locus_tag="JN701_001227"
     CDS             5903..6274
                     /gene="rplN"
                     /locus_tag="JN701_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY7363740.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6285..6599
                     /gene="rplX"
                     /locus_tag="JN701_001228"
     CDS             6285..6599
                     /gene="rplX"
                     /locus_tag="JN701_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY7363741.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6614..7153
                     /gene="rplE"
                     /locus_tag="JN701_001229"
     CDS             6614..7153
                     /gene="rplE"
                     /locus_tag="JN701_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY7363742.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7168..7473
                     /gene="rpsN"
                     /locus_tag="JN701_001230"
     CDS             7168..7473
                     /gene="rpsN"
                     /locus_tag="JN701_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY7363743.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7507..7899
                     /gene="rpsH"
                     /locus_tag="JN701_001231"
     CDS             7507..7899
                     /gene="rpsH"
                     /locus_tag="JN701_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY7363744.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7912..8445
                     /gene="rplF"
                     /locus_tag="JN701_001232"
     CDS             7912..8445
                     /gene="rplF"
                     /locus_tag="JN701_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY7363745.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8455..8808
                     /gene="rplR"
                     /locus_tag="JN701_001233"
     CDS             8455..8808
                     /gene="rplR"
                     /locus_tag="JN701_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY7363746.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8823..9326
                     /gene="rpsE"
                     /locus_tag="JN701_001234"
     CDS             8823..9326
                     /gene="rpsE"
                     /locus_tag="JN701_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY7363747.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9330..9509
                     /gene="rpmD"
                     /locus_tag="JN701_001235"
     CDS             9330..9509
                     /gene="rpmD"
                     /locus_tag="JN701_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY7363748.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9513..9947
                     /gene="rplO"
                     /locus_tag="JN701_001236"
     CDS             9513..9947
                     /gene="rplO"
                     /locus_tag="JN701_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY7363749.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9955..11286
                     /gene="secY"
                     /locus_tag="JN701_001237"
     CDS             9955..11286
                     /gene="secY"
                     /locus_tag="JN701_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY7363750.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11318..11434
                     /gene="rpmJ"
                     /locus_tag="JN701_001238"
     CDS             11318..11434
                     /gene="rpmJ"
                     /locus_tag="JN701_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY7363751.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11581..11937
                     /gene="rpsM"
                     /locus_tag="JN701_001239"
     CDS             11581..11937
                     /gene="rpsM"
                     /locus_tag="JN701_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY7363752.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11954..12343
                     /gene="rpsK"
                     /locus_tag="JN701_001240"
     CDS             11954..12343
                     /gene="rpsK"
                     /locus_tag="JN701_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY7363753.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12377..12997
                     /gene="rpsD"
                     /locus_tag="JN701_001241"
     CDS             12377..12997
                     /gene="rpsD"
                     /locus_tag="JN701_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY7363754.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13023..14012
                     /gene="rpoA"
                     /locus_tag="JN701_001242"
     CDS             13023..14012
                     /gene="rpoA"
                     /locus_tag="JN701_001242"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY7363755.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14053..14436
                     /gene="rplQ"
                     /locus_tag="JN701_001243"
     CDS             14053..14436
                     /gene="rplQ"
                     /locus_tag="JN701_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY7363756.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14543..14911
                     /locus_tag="JN701_001244"
     CDS             14543..14911
                     /locus_tag="JN701_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAY7363757.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14922..15347
                     /gene="zntR"
                     /locus_tag="JN701_001245"
     CDS             14922..15347
                     /gene="zntR"
                     /locus_tag="JN701_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAY7363758.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15403..15621
                     /gene="arfA"
                     /locus_tag="JN701_001246"
     CDS             15403..15621
                     /gene="arfA"
                     /locus_tag="JN701_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HAY7363759.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="JN701_001247"
     CDS             complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="JN701_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAY7363760.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="JN701_001248"
     CDS             complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="JN701_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY7363761.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="JN701_001249"
     CDS             complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="JN701_001249"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY7363762.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="JN701_001250"
     CDS             complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="JN701_001250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY7363763.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19853..20362)
                     /gene="def"
                     /locus_tag="JN701_001251"
     CDS             complement(19853..20362)
                     /gene="def"
                     /locus_tag="JN701_001251"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY7363764.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20492..21616
                     /gene="dprA"
                     /locus_tag="JN701_001252"
     CDS             20492..21616
                     /gene="dprA"
                     /locus_tag="JN701_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY7363765.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21588..22061
                     /locus_tag="JN701_001253"
     CDS             21588..22061
                     /locus_tag="JN701_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY7363766.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22090..22632
                     /locus_tag="JN701_001254"
     CDS             22090..22632
                     /locus_tag="JN701_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363767.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22637..23209
                     /gene="tsaC"
                     /locus_tag="JN701_001255"
     CDS             22637..23209
                     /gene="tsaC"
                     /locus_tag="JN701_001255"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY7363768.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23214..24032
                     /gene="aroE"
                     /locus_tag="JN701_001256"
     CDS             23214..24032
                     /gene="aroE"
                     /locus_tag="JN701_001256"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY7363769.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24029..24286
                     /locus_tag="JN701_001257"
     CDS             24029..24286
                     /locus_tag="JN701_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY7363770.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24262..24816)
                     /locus_tag="JN701_001258"
     CDS             complement(24262..24816)
                     /locus_tag="JN701_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY7363771.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6457 a         5996 c         6569 g         6220 t
ORIGIN      
        1 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
       61 cgcaagcgaa tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg
      121 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
      181 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgtc ccccataacg
      241 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
      301 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
      361 ataaacaagc gtacaaaatc agaaagggaa gggtagtgtc cataaaacat cctctgtaat
      421 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta
      481 acctgattcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc
      541 gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta
      601 taatgcgcgg gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt
      661 tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc
      721 aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct
      781 catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca
      841 agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat
      901 cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga
      961 cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac
     1021 cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat
     1081 cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc
     1141 ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc
     1201 gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac
     1261 cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct
     1321 ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct
     1381 gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa
     1441 aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg
     1501 aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct
     1561 atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg
     1621 ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac
     1681 ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca
     1741 gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct
     1801 gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt
     1861 tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt
     1921 aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc
     1981 tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca
     2041 cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact
     2101 ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa
     2161 gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga
     2221 gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc
     2281 aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc
     2341 tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg
     2401 cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta
     2461 ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt
     2521 gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga
     2581 cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag
     2641 aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta
     2701 aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc
     2761 acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca
     2821 acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg
     2881 ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc
     2941 cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc
     3001 tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca
     3061 aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
     3121 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
     3181 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
     3241 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
     3301 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga
     3361 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
     3421 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
     3481 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
     3541 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
     3601 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
     3661 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
     3721 atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
     3781 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc
     3841 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
     3901 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca
     3961 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
     4021 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     4081 gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt
     4141 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     4201 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     4261 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     4321 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     4381 tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     4441 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     4501 ggacgcaaaa ctggttgctg acagcatcac ttcccagctg gaacgtcgcg ttatgttccg
     4561 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     4621 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     4681 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     4741 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     4801 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     4861 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     4921 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     4981 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     5041 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     5101 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     5161 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     5221 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     5281 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc
     5341 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     5401 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     5461 ctgaacgaga aggctggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     5521 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     5581 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     5641 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     5701 ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatacg
     5761 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     5821 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     5881 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     5941 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     6001 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     6061 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     6121 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     6181 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     6241 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     6301 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     6361 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     6421 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     6481 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     6541 gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     6601 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     6661 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     6721 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     6781 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     6841 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     6901 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     6961 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     7021 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     7081 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     7141 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     7201 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     7261 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     7321 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     7381 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     7441 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     7501 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     7561 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     7621 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     7681 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     7741 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     7801 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     7861 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     7921 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     7981 attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt
     8041 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     8101 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     8161 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg taatgtgatt
     8221 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     8281 gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag
     8341 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt
     8401 tacgccgacg aagtcgtgcg tatcaaagag gctaagaaga agtaaggtaa cactatggat
     8461 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     8521 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     8581 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     8641 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     8701 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     8761 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     8821 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     8881 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     8941 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
     9001 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
     9061 ctctgcaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
     9121 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
     9181 ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
     9241 ctattgatgg cctggaaaat atgaattctc cagaaatggt cgctgccaag cgtggtaaat
     9301 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
     9361 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc
     9421 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
     9481 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
     9541 aaggctccaa aaaggcgggt aaacgcttgg gtcgtggtat cggttctggc ctcggtaaaa
     9601 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
     9661 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
     9721 aagcagcgat tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg
     9781 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
     9841 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
     9901 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
     9961 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
    10021 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
    10081 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
    10141 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc
    10201 atgccgtata tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg
    10261 gcggaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgctac
    10321 ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg
    10381 cctggtatgc aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta
    10441 agtctggtca caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt
    10501 atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc
    10561 attgcccata ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg
    10621 gttgcagtat tagtatttgc agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc
    10681 cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc
    10741 acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt
    10801 attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg
    10861 ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg
    10921 tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca
    10981 gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg
    11041 gcgaagtata tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc
    11101 tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt
    11161 gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact
    11221 ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc
    11281 cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa
    11341 gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag
    11401 tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag
    11461 ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg
    11521 agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata
    11581 gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact
    11641 tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct
    11701 gaagatgtta agatcagtga gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt
    11761 gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg
    11821 atggatcttg gttgctatcg cggcttgcgt catcgtcgtg gtctcccggt tcgcggtcag
    11881 cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg
    11941 gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt
    12001 ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga
    12061 tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg
    12121 caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga
    12181 atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac
    12241 tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat
    12301 ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt
    12361 ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga
    12421 gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga
    12481 acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt
    12541 gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta
    12601 ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga
    12661 aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg
    12721 tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaaca tcgcttctta
    12781 tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt
    12841 gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc
    12901 tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat
    12961 taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca
    13021 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
    13081 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
    13141 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
    13201 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
    13261 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
    13321 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
    13381 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
    13441 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc
    13501 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
    13561 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
    13621 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
    13681 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
    13741 ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc
    13801 tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg
    13861 atctggtaca acgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc
    13921 ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa
    13981 actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag
    14041 aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat
    14101 cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg
    14161 actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag
    14221 actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg
    14281 gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt
    14341 attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt
    14401 gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg
    14461 ccccggcggg tttttttata cccgtagcat ccccacttat ctacaatagc tgtactcttt
    14521 ttgttcatcc cctggagtat ttatgtggtt acttgaccag tgggcagagc gtcatatagc
    14581 agaagcgcaa gcgaaaggtg agtttgataa tctggcaggt agcggcgaac cattgatact
    14641 ggatgatgat tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc
    14701 tggttgctta ccgccagaac ttgagcagcg gagggaagca attcagcttc tggatatcct
    14761 gagaggtatc cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact
    14821 ggaattgaag ctgcgacaag ctggatttag taccgatttt ttacgcggcg attatgctga
    14881 caagttgttg gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc
    14941 tggcaaaaat ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga
    15001 tggagcatga agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc
    15061 gattgaaatt tatccgccat gctagacaac taggtttcag tctggagtcg atccgcgagt
    15121 tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc
    15181 aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct
    15241 tgcaacgcct taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgttcgattc
    15301 ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg
    15361 caggattaca ctcgcgccgt aaaataacca attggagttt ttatgagccg atatcagcat
    15421 actaaagggc agataaggga taatgcgata gaagcattac tacatgatcc cttattccga
    15481 cagcgcgtag agaaaaataa gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc
    15541 aatcggggga actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt
    15601 ctgctttcag gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct
    15661 gccagtaata cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga
    15721 ttcagtttgt tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca
    15781 aagacgtttt gaatgaagac accgtaatgc atcacaacag cagggatatc cccctgcgca
    15841 tcgcgtagcg tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt
    15901 ggcatgatga tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg
    15961 acacccaccg ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata
    16021 atgctcatgt tattctccct atgaagccga caaccataag tctaacaaat gttaagccat
    16081 tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc
    16141 attagacgcc ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg
    16201 gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga
    16261 ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc
    16321 ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga
    16381 gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct
    16441 gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata
    16501 tcgatcacgc tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc
    16561 tttttcgcac ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg
    16621 gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc
    16681 gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc
    16741 tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct
    16801 gcaccgatat taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt
    16861 aattcactca tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca
    16921 gcttcaacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc
    16981 acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc
    17041 gggccgccat aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc
    17101 acctgcaatg cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct
    17161 ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt
    17221 acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct
    17281 acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg
    17341 tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc
    17401 aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata
    17461 tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga
    17521 atgataattt tcatcagtct cgacccgtta tctcatcact ttttgattag cttagcgtaa
    17581 aagaagccgt cgccctcttc ggcaccaggc agattttgtt tacccggttg ctctggtgtt
    17641 cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aagctttaat ctgcaggcta
    17701 ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc
    17761 ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg
    17821 cgacgtaacc atttaatatc cggatggcga cgaatcaccc ccgttgctga acaaggcgca
    17881 tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca
    17941 ccttgtttca cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg
    18001 cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc
    18061 gttgttttac cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca
    18121 agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa
    18181 ccaggtagcg catgaacagg tgcaggtgtt tccagacgta cagcatcagg gtaatccgca
    18241 tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga
    18301 tgcgtacgat taacgcgcag ccacattggc ggacgctggt tattggcttc gacgatggat
    18361 tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa
    18421 cgtgcatcac tggcattaaa cttggctaat aactcttctt gctgacgctg gaactggcgt
    18481 aatacaccgt taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc
    18541 gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc
    18601 ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta
    18661 atcagccagt ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt
    18721 gctttgtctt tatcagaaac tttttgctgg agcggtggca gaatgttgct taatgattgc
    18781 ccttgctcga cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt
    18841 ttcataagta taaaaatgcc cggtaagacc gggcttagaa gagtggacta tcaggccaga
    18901 cggttgcccg gaacaaacca ttcccgacga gaattcagga ggtcttgcgc gctcatcgct
    18961 ttcttacccg caggttgtaa cgagagcaga ttcaggattc catcaccagt tgcaacctga
    19021 atgccttgtt tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg
    19081 accgacgctt tccagacttt aaccggctgt ctttcaattt ccagccagct cattggccat
    19141 ggattgaaag cgcgaatgta gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc
    19201 gcttcttctt tactcaactt ttcggcgtaa gtgacaagag tttcgtcctg aacttctggt
    19261 ttcgccgtgc cgtctgccag ttgtttcaac gtggtgataa gcccttgtgg gccaagctct
    19321 gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta
    19381 tagagcatgt caccggtgtc taaaccgaca tccatttgca taatggtcac accagtttct
    19441 gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt
    19501 gaaccatgaa cgttgataca gccaagacgc ggcatctcca gcactgcttt aggcagaatt
    19561 aagccatagg cgacgacgac cataacatca gcctgcagat cggcgaccag ttgctggttt
    19621 tcttgtggac gcagggaaac aggttgaaaa acgggcagac ctttttcctc agccagaact
    19681 ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac
    19741 acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca
    19801 ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc
    19861 gggctttcag acgatccagt ttttcaactt tctgacgaat acgttgttgt ttcaacggtg
    19921 acagataatc cataaacagt ttgccgacca ggtgatccat ctcatgctga atacagatgg
    19981 ctaacagacc gtctgcttcc aattcaaatg gtttaccgtc gcggtccagg gcgcgaattt
    20041 taactttctc tgcgcgtggc actaaagcac gttgttcagg gatcgacagg caaccttctt
    20101 caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt
    20161 cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg
    20221 ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa
    20281 tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat
    20341 gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat
    20401 ttcttctatt ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat
    20461 tgctaaggct gcttatggca gggagataag gatggtcgat acagatattt ggctgcgttt
    20521 aatgagcatc agcagcttgt acggcgatga tatggtccgt atagctcact ggctggcaaa
    20581 acagtcgcat attgatgcgg ttgtattgca gcaaacaggg cttacattgc ggcaggcaca
    20641 acgctttctt tcatttccgc gaaagagtat cgaaagctca ctttgttggt tggagcaacc
    20701 caaccatcat ttaatccctg cggacagcga attttatcct cctcaacttc tggcgacgac
    20761 agattacccc ggcgcactgt ttgttgaagg agaactgtac gcgctgcatt catttcagct
    20821 tgccgtagtg gggagtcggg cgcattcatg gtatggcgag cgatggggac gattattttg
    20881 cgaaactctg gccacgcgtg gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg
    20941 tgtggcgcat aaagcggcct tacaggtaaa tggcgtcagc attgctgtat tggggaacgg
    21001 acttaatacc attcatccgc gccgccatgc ccgactggct accagtttgc ttgaacatgg
    21061 tggggcactt gtctcggaat ttcccctcga tgtttcaccc cttgcttaca atttcccacg
    21121 aagaaatcgc attatcagtg gtctaagtaa aggtgtactg gtggtggaag cggctttgcg
    21181 cagtggttcg ttggtgacag cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt
    21241 gccaggacca atagggaatc cgggaagcga agggcctcac tggttaataa aacaaggtgc
    21301 gattcttgtg acggaaccgg aagaaattct ggaaaacttg caatttggat tgcactggtt
    21361 gccagacgcc cctgaaaatt cattttattc accagatcag caagacgtgg cattgccatt
    21421 tcctgagctc ctggctaacg taggagatga ggtaacacct gttgacgtcg tcgctgaacg
    21481 tgccggccaa cctgtgccag aggtagttac tcaactactc gaactggagt tagcaggatg
    21541 gatcgcagct gtacccggcg gctatgtccg attgaggagg gcatgccatg ttcgacgtac
    21601 taatgtattt gtttgaaacc tatattcaca cagaagctga gttgcgtgtg gatcaagaca
    21661 aacttgaaca ggatcttacc gacgcaggat ttgatcgaga agatatctac aatgccctgc
    21721 tttggctgga aaagcttgcg gattatcagg aaggactgac agaaccgatg caactggcct
    21781 ctgatccact ctccatgcgt atttataccc ctgaggagtg tgaaagactg gatgccagct
    21841 gtcgtggttt tctgcttttc cttgagcaaa ttcaggtgct taaccttgaa atccgtgaaa
    21901 tggtgataga gcgagtgctc gcgctggata ccgcagagtt cgacctggaa gacctgaaat
    21961 gggtaatcct gatggtgttg ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg
    22021 aagaattact ctttgaagtg aatgaaggta tgctgcatta atcatttctt taattcagca
    22081 taagttgtta tggcgaaatc agcactgttc acggtgcgta ataatgagtc ctgcccaaag
    22141 tgcggggctg aactggttat tcgatccggg aaacacggtc cgtttcttgg atgctcacag
    22201 tatccggcgt gtgactacgt ccgtcctctg aaatcttcag cggatggaca tattgtcaaa
    22261 gttctggagg ggcaggtttg ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc
    22321 tttggtatgt ttattggttg cagtaactac cctgaatgcg aacataccga acttatcgat
    22381 aaaccggacg aaacagcaat tacatgcccc caatgtcgga cgggccatct ggtccagcgc
    22441 cgctcccgtt atggcaaaac atttcactcc tgtgatcgct acccggagtg tcaatttgcc
    22501 attaacttca aacccatagc tggagaatgc cctgagtgtc attatccact actcatcgaa
    22561 aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta aacaatgtgg aaagccggtt
    22621 tcggcggaat aataacgtga ataataacct gcaaggagac gctatcgcag ctgcgataga
    22681 tgtcctcaat gaagaacgtg tcatcgccta tccaacggaa gccgttttcg gtgttgggtg
    22741 cgatcctgat agcgaaacag cagtgatgag actgttggag ttaaaacagc gtccggttga
    22801 taaggggctg attttaatcg cggcaaatta cgagcagctt aaaccctata ttgatgacac
    22861 catgctgact gatgcgcagc gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt
    22921 tgtctttccc gcgcctgcga caacaccgcg ctggttgacg ggccgctttg attcgcttgc
    22981 tgtacgagtc accgaccatc cgttggtggt tgctttgtgc caggcttatg gtaaaccgct
    23041 ggtttctacc agtgccaact tgagtggatt gccaccttgt cgaacagtag acgaagttcg
    23101 cgcacaattt ggcgcggcgt tcccggttgt gcttggtgaa acgggggggc gtttaaatcc
    23161 ttcagaaatc cgcgatgccc tgacgggtga actgtttcga caggggtaac ataatggaaa
    23221 cctatgctgt ttttggtaat ccgatagccc acagcaaatc gccattcatt catcagcaat
    23281 ttgctcagca actgaatatt gaacatccct atgggcgcgt gttggcaccc atcaatgatt
    23341 tcatcaacac actgaacgct ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac
    23401 cttttaaaga agaggctttt gccagagcgg atgagcttac tgaacgggca gcgttggctg
    23461 gtgctgttaa taccctcaag cggttagaag atggacgctt gcagggtgac aataccgatg
    23521 gtataggttt gttaagcgat ctggaacgtc tgtcttttat ccgtccgggt ttacgtattc
    23581 tgcttatcgg cgctggtgga gcatctcgcg gcgtactact accactcctt tccctggact
    23641 gtgcggtgac aatcactaat cggacggtat cccgtgcgga agagttggct aaattgtttg
    23701 cgcacactgg cagtattcag gcgttgggta tggacgaact ggaaggtcat gagtttgatc
    23761 tcattattaa tgcaacatcc agtggcatca gtggtgatat tccggcgatc ccgtcatcgc
    23821 tcattcatct aggcatttat tgctatgaca tgttctatca gaaaggaaaa actccttttc
    23881 tggcatggtg tgagcagcga ggctcaaagc gtaatgctga tggtttagga atgctggtgg
    23941 cacaggcggc tcatgccttt cttctctggc acggtgttct gcctgacgta gaaccagtta
    24001 taaagcaatt gcaggaggaa ttgtccgcgt gaatcaggcc atccagtttc cggacaggga
    24061 agagtgggac gagaataaaa aatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact
    24121 gacatgcgcg atctctggcg agagtctggc gtatcgcttt actggagata cgccagaaca
    24181 gtggttagcg agttttcgtc agcatcgctg ggacctggaa gaagaagcgg aaaacttaat
    24241 tcaggaacaa agtgaagatg atcaaggctg ggtctggtta ccctgatcca gatattcgtc
    24301 cttccatttc acgtaattat tcgcggaata gcgtaaccca gccttctctt catcacttaa
    24361 cgggcggatc tgtttgacgg ggctaccgag atacagatat ccgctctcca gccgtttatt
    24421 ttgtgggacc agactacccg caccaatcat cacatcatct tctactattg cgccatcaag
    24481 taaaattgag cccatcccaa ccaaaactcg attgccaatg gtgcagccgt ggagcatcac
    24541 cttgtgacca acagtgacat cttcgccaat ggttaatggg ttgccatctg ggttgtacga
    24601 ggatttatga gtgacatgca acatactgcc atcctggata ttggtgcgtg ctccgatctg
    24661 tacataatgt acatctccac gaatcacaac gagcggccag atccccacat catcagccag
    24721 acgaacgcca ccaatcacga cactgctatc gtcgatcatt acgcgctgac cgatttgtgg
    24781 aaaaagatcg cggtatgggc gtaaaacatc agacatactt acctcagcaa taaatgattt
    24841 actaatgact ttgggggcat tattggcctt gtgcaagtct tttagtatgc aaaaaagcac
    24901 cgttttgtgt gcgattgcag caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa
    24961 aaaaatactt gtgcaaaata ttgggatccc tataatgcgc ctccgttaag acgacaacgt
    25021 gaaacacttc acaggatggt cggaacaacg aagagaaaaa atcctgaaat tcagggttga
    25081 ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgttg
    25141 caactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggattctt
    25201 aacgtcgcaa gacgaaaaat gaataccaag tctcaagagt ga
//
LOCUS       DADLTD010000038        25119 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLTD010000038 DADLTD010000000
VERSION     DADLTD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.038"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..393
                     /gene="hyfA"
                     /locus_tag="JN701_001259"
     CDS             <1..393
                     /gene="hyfA"
                     /locus_tag="JN701_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY7363772.1"
                     /translation="QLNESLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQADGSL
                     KDGEENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVRACPNQALRLITDDSLQR
                     QMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            393..2410
                     /gene="hyfB"
                     /locus_tag="JN701_001260"
                     /pseudo
     CDS             393..2410
                     /gene="hyfB"
                     /locus_tag="JN701_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            2421..3368
                     /gene="hyfC"
                     /locus_tag="JN701_001261"
     CDS             2421..3368
                     /gene="hyfC"
                     /locus_tag="JN701_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY7363773.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            3385..3495
                     /locus_tag="JN701_001262"
     CDS             3385..3495
                     /locus_tag="JN701_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HAY7363774.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            3507..4614
                     /locus_tag="JN701_001263"
                     /pseudo
     CDS             3507..4614
                     /locus_tag="JN701_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            4626..5276
                     /gene="hyfE"
                     /locus_tag="JN701_001264"
     CDS             4626..5276
                     /gene="hyfE"
                     /locus_tag="JN701_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY7363775.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            5281..6861
                     /gene="hyfF"
                     /locus_tag="JN701_001265"
                     /pseudo
     CDS             5281..6861
                     /gene="hyfF"
                     /locus_tag="JN701_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
     gene            6851..8566
                     /gene="hyfG"
                     /locus_tag="JN701_001266"
     CDS             6851..8566
                     /gene="hyfG"
                     /locus_tag="JN701_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HAY7363776.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            8576..9115
                     /gene="hyfH"
                     /locus_tag="JN701_001267"
     CDS             8576..9115
                     /gene="hyfH"
                     /locus_tag="JN701_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY7363777.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            9112..9870
                     /gene="hyfI"
                     /locus_tag="JN701_001268"
     CDS             9112..9870
                     /gene="hyfI"
                     /locus_tag="JN701_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY7363778.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            9863..10276
                     /gene="hyfJ"
                     /locus_tag="JN701_001269"
     CDS             9863..10276
                     /gene="hyfJ"
                     /locus_tag="JN701_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY7363779.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            10306..12318
                     /locus_tag="JN701_001270"
                     /pseudo
     CDS             10306..12318
                     /locus_tag="JN701_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            12340..13046
                     /locus_tag="JN701_001271"
                     /pseudo
     CDS             12340..13046
                     /locus_tag="JN701_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            12997..13185
                     /locus_tag="JN701_001272"
     CDS             12997..13185
                     /locus_tag="JN701_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAY7363780.1"
                     /translation="MPFAISPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIG
                     GAVLVSMCYQAIYLRQES"
     gene            complement(13223..14284)
                     /locus_tag="JN701_001273"
     CDS             complement(13223..14284)
                     /locus_tag="JN701_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7363781.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCPRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            14497..15960
                     /gene="bepA"
                     /locus_tag="JN701_001274"
     CDS             14497..15960
                     /gene="bepA"
                     /locus_tag="JN701_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY7363782.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            15981..16340
                     /gene="arsC"
                     /locus_tag="JN701_001275"
     CDS             15981..16340
                     /gene="arsC"
                     /locus_tag="JN701_001275"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY7363783.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(16478..17224)
                     /gene="hda"
                     /locus_tag="JN701_001276"
     CDS             complement(16478..17224)
                     /gene="hda"
                     /locus_tag="JN701_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY7363784.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(17274..18563)
                     /gene="uraA"
                     /locus_tag="JN701_001277"
     CDS             complement(17274..18563)
                     /gene="uraA"
                     /locus_tag="JN701_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY7363785.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(18649..19275)
                     /gene="upp"
                     /locus_tag="JN701_001278"
     CDS             complement(18649..19275)
                     /gene="upp"
                     /locus_tag="JN701_001278"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY7363786.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            19599..20636
                     /gene="purM"
                     /locus_tag="JN701_001279"
     CDS             19599..20636
                     /gene="purM"
                     /locus_tag="JN701_001279"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY7363787.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            20636..21274
                     /gene="purN"
                     /locus_tag="JN701_001280"
     CDS             20636..21274
                     /gene="purN"
                     /locus_tag="JN701_001280"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY7363788.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            21446..23512
                     /gene="ppk1"
                     /locus_tag="JN701_001281"
     CDS             21446..23512
                     /gene="ppk1"
                     /locus_tag="JN701_001281"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY7363789.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            23517..25058
                     /gene="ppx"
                     /locus_tag="JN701_001282"
     CDS             23517..25058
                     /gene="ppx"
                     /locus_tag="JN701_001282"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY7363790.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         5722 a         6575 c         6679 g         6143 t
ORIGIN      
        1 caactcaacg aaagcctctg tattggctgc aagctttgcg ccgtggtctg cccatttggc
       61 gcaatcagcg cttcaggaag ccgtccggtg aatgcccctg cgcaatatgt ttttcaggct
      121 gacggctcac tcaaagatgg cgaagaaaac gcgccgaccc aacatgcttt gctgcgctgg
      181 gaaccgggtg tccagaccgt cgcggtgaaa tgcgacctgt gtaatttctt gccagaaggt
      241 ccggcctgcg ttcgcgcttg cccgaatcag gcgttacggc tgattaccga cgatagcctg
      301 caacgtcaga tgaaagaaaa acagcgcctt gccgcaagct ggtttgccaa tggcggggag
      361 gatccccttt ccctcactca ggagcaacac taatggatgc cctgcaatta ttaacctggt
      421 cgctgatcct ctttctgttt gccagtctgg cttcgctgtt tttactcggt ctggacagac
      481 tggctattaa gctttccggc atcacatcgc tggtgggcgg cgtgattggc atcatcagcg
      541 gaattacgca attacatgct ggtgtaactt tagtcgcccg ttttgccacc ccttttgact
      601 ttgccgattt aaccctgcga atggatagcc tctcggtatt tatggtgctg gttatctcct
      661 tgctggtggt ggtttgttcg ctctattcat tgacttatat gcgcgaatac gagggcaaag
      721 gcgcggcggc gatgggcttc tttatgaatc ttttcatcgc atcgatggtt gccctgctgg
      781 tgatggacaa cgctttttgg ttcatcgtgc tgtttgaaat gatgtcgctg tcttcctggt
      841 ttctggtcat tgccaggcag gataaaacgt cgatcaacgc tggcatgctc tactttttta
      901 tcgcccacgc cggatcggtg ctgattatga tcgccttctt gctgatgggg cgcgaaagcg
      961 gcagcctcga ttttgccagt ttcgcacgct ttcactttct ccggggctgg cgtcggcgat
     1021 gtttctgctg gcctttttcg gttttggcgc gaaagccggg atgatgccgt tgcacagctg
     1081 gttgccgcgc gctcaccctg ccgcaccatc gcacgcttcg gcgttgatgt ctggcgtaat
     1141 ggtcaaaatc ggtattttcg gcatcctgaa agtagcgatg gatctgctgg cgcaaacggg
     1201 tttgcctctg tggtggggca ttctggtgat ggcgatcggc gcaatctccg cgctcctggg
     1261 cgtgctatat gcgctggcgg aacaggatat caaacggctg ctggcctgga gtaccgtcga
     1321 aaacgtcggc attattttgc tggcagtcgg tgtggcgatg gacggtctgt cactgcacga
     1381 cccgctgctc accgtggttg gactgctcgg cgcactgttt catctgctca accatgcgct
     1441 gttcaaaggg ctgctatttc tcggcgcggg cgcgattatt tcgcgtttgc atacccacga
     1501 catggaaaaa atgggggcac tggcgaaacg gatgccgtgg acagccgcag catgcctgat
     1561 tggttgcctc gcgatatcag ccattcctcc gctgaatggt tttatcagcg aatggtacac
     1621 ctggcagtcg ctgttctcac taagtcgtgt ggaagccgta gcgctacaac ttgcgggtcc
     1681 tattgctatg gtaatgctgg cagtcactgg tgggctggca gtaatgtgct tcgtcaaaat
     1741 gtacggtatt actttctgtg gtgcgccgcg cagtacacac gctgaagagg cacaggaagt
     1801 gccaaatacg atgatcgtcg ccatgctact gctcgcggca ctctgcgtat taattgcgct
     1861 tagtgccagt tggctggcac cgaagataat gcatattgcc catgcgttta ccaatacccc
     1921 tcccgtcact gtcgccagcg gaatagcact tgtacccggc acgtttcata cacgggtcac
     1981 tccttcatta ctgttgctgt tactactggc gatgcctttg ctgcctggcc tttactggct
     2041 gtggtgtcgt tcgcgccgcg cagcgtttcg tcgcacagga gatgcctggg catgcggcta
     2101 cggctgggaa aacgcgatgg ctccgtcagg caatggcgtg atgcagccgc tgcgtgtggt
     2161 cttctctgcg ttatttcgtc tacgacaaca gctcgaccct acgctgaggc taaataaaga
     2221 tcttgcgcac gtcaccgcca gggctcagag cacagaaccc ttctgggatg agcgggtgat
     2281 ccgccccatc gtgagcgcca cccaacggct ggccaaagaa atacagcatc tgcaaagcgg
     2341 cgactttcgt ctctattgcc tgtatctagt tgccgcactg gttgtgctgc taatcgctat
     2401 tgccgtctaa ggaaatcacc atgagacaaa ctctttgcga cggatatctg gtcatttttg
     2461 cgttagcaca ggccgtgatt ctgctgctgc taaccccgct ttttacgggt atttcccggc
     2521 agatacgcgc gcgtatgcac tcccgccgcg ggccggggat ctggcaggat tatcgcgaga
     2581 tccacaaact gtttaaacgc caggaagttg cgcctatatc ttcaggtctg atgttccgcc
     2641 tgatgccgtg ggtattaatc agcagcatgc tggtgctggc gatggcccta ccacttttta
     2701 ttaccgtttc cccttttgcg ggcggcggcg atctgatcac ccttatctat cttcttgccc
     2761 tgtttcgttt tttctttgct ctttccggac tggataccgg aagcccgttt gcgggagtcg
     2821 gtgccagtcg cgagttgacg ctcggcattc tggtcgaacc catgctcatt ctctcactgc
     2881 tggtattggc gctgatagca ggttccacgc atatcgagat gatcagcaat acgctggcaa
     2941 cgggctggaa ctcgccgcta accaccgtac tggcgttact ggcctgtggt tttgcctgct
     3001 tcattgagat gggaaaaatt ccctttgatg ttgctgaagc agaacaggaa ttacaggaag
     3061 gcccgctgac cgaatattcc ggtgccgggc tggcgctagc gaaatggggg ctggggctga
     3121 aacaggtcgt gatggcagca ctgtttgtgg ccctgtttct gccctttggg cgcgcgcaag
     3181 aactttctct cgcctgcctg ctgacttcac ttgtcgttac gctgctcaag gttttgctga
     3241 tttttgtact ggcctcaatc gcagaaaaca cgctggcacg cgggcgtttt ttactcattc
     3301 accatgtgac ctggcttggc ttcagccttg ctgcgcttgc atgggtcttc tggttaaccg
     3361 gtctgtaagg agcactgacg gaatatggaa aatcttgctc tgacgacgtt aatgctgcct
     3421 tttatcggcg cactggtcgt ttcgttttcg ccacaacgtc gggccgccga atgggggttt
     3481 tgttcgccgc actgaccacg ctgtgcatgt tgtcgctgat ctccgcgttt tatcaggccg
     3541 ataaagttgc cgtcacgttg acgttggtca acgtggggga tgtggcattg tttggcctgg
     3601 tcattgatcg cgtgagtacg ctgattctgt ttgtggtggt gtttctcggt ttgctggtca
     3661 cgatctactc cacgggttat ctgacggata aaaatcgcga acacccgcat aacggcacga
     3721 atcgttatta cgcatttttg ctggtgttta tcggcgcgat ggcgggactg gtactctcct
     3781 cgacgctgct cggtcagttg ttgttttttg aaattagggc ggctgctcct gggcgttgat
     3841 cagttattac cagagcgata aagcgcagcg ttcaacacta aaagcgttgc ttatcaccca
     3901 tatcggttcg ttggggttgt atcttgccgc cgccacgctg tttttgcaga ccggaacgtt
     3961 tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt tatctggttt atggcggcat
     4021 cctgtttgcc gcgtggggga aatcggccca gctaccgatg caagcgtggc taccggacgc
     4081 aatggaagcg ccaacaccga tcagagccta tctccacgcc gcatcgatgg tgaaagtggg
     4141 cggctggcac tggagggcag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg
     4201 tttttccttg tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg
     4261 cgtggcgtat tgcactccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggcg
     4321 attaccggtg tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt
     4381 tttgcgttgt cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa
     4441 tcggtcgcca gtttcgcctg gtttattcgc tggtttggtc gcgttgtgcc tggcaaaccg
     4501 agcgaggccg tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta
     4561 ctgattgtga tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat
     4621 gatgaatgat cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc
     4681 tgtttgtgat tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc tgccagtcgc
     4741 tggtgctggt gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga
     4801 tctggtccgc cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg
     4861 ctgcacgaaa tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg
     4921 cactgctcgc ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac
     4981 cgatggctac cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc
     5041 tgctgtgcat tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg
     5101 aaaacggctc ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa
     5161 taggtatcgc taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa
     5221 tatggcgtac ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag
     5281 atgagttatt ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc
     5341 tgttttgcct gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc
     5401 ttagggatca cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca
     5461 ggagaaatat tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc
     5521 gccattcttg gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat
     5581 gaagtggcac acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat
     5641 ctgtttttgt tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg
     5701 gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca
     5761 tcgctggaat cagcatggaa gtacatcatt atttgtactg ttgatgtcgc ttttggtctg
     5821 ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg
     5881 atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg
     5941 gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc
     6001 tagctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta
     6061 ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaagccatc
     6121 ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg
     6181 gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag
     6241 aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg
     6301 ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat
     6361 gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg
     6421 ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc
     6481 aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg
     6541 attatcgtcc tgctgttatt gctgttaacg ctggtgctgg cgggcctggt acggatggct
     6601 gcgcgggtgt taatggcgaa accgccgcag gccgttaacc ggggtgatct cggctggttg
     6661 accacctcgc caatggtgat tctgctggtc atgatgctgg cgatgggaac gcatattcca
     6721 caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac
     6781 ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg
     6841 gagaaacaca gtgaacgtta attcatcgtc aaatagtggc gaagcgattc tcgccgccct
     6901 gaaaacgcag ttccccggcg cggtactgga tgaagagcga caaacgcctg aacaggtcac
     6961 cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga
     7021 tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt
     7081 ttattatgcc ctttcaatgg aaggagccga aaaatgctgg atcgtggtga aggcactggt
     7141 cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg
     7201 cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg
     7261 cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcacaaag atgcgatgga
     7321 ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg
     7381 caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctccg atgaaccggg
     7441 tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta
     7501 tgtccatcga ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt
     7561 cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc
     7621 ggttgaaaat gcactgggga ttgaggtgcc gcaacgagcg cataccattc gctcgattct
     7681 gctggaagtc gaacggctac acagtcattt gctcaacctt ggcctctcct gccattttgt
     7741 tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc
     7801 ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg
     7861 cgatattctc aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgcgccga
     7921 cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg
     7981 cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg
     8041 cggcagtggt tttgcccgtg atttgcgctt tgatcacccc tacgccgact acggcaatat
     8101 tccaaaaaca ctgtttacct tcaccggcgg cgatgttttc tcccgcgtga tggtccgtgt
     8161 caaagagacg tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca tgccggatac
     8221 cccactgctg accgaaggct ttagctataa acctcacgca ttcgcgctgg gctttgttga
     8281 agcgccacgc ggtgaagacg tgcactggag catgctcggt gataaccaaa aattgttccg
     8341 ctggcgctgc cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca tgctgcgcgg
     8401 caataccgtt tctgacgcac cgctgattat cggtagcctt gatccctgct actcctgtac
     8461 cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc cgtataaaga
     8521 gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg aagtaaggac agaagatgct
     8581 gaagttactg aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc ccttcgcgcc
     8641 actggaggtc agcccaggct ttcgcgggaa accggacctg atgcccagcc aatgtattgc
     8701 ctgcggcgcc tgtgcttgtc cggcaaatgc gctgactatc cagaccgacg accagcaaaa
     8761 ttcgcgcacc tggcagctct atctggggcg ttgtatttac tgcggacgtt gtgaagaagt
     8821 gtgcccgacc agagccatcc agcttaccaa taactttgaa ctgaccgtca ccaataaagc
     8881 cgatctctat acccgcgcga cgttccatct acaacgttgc agccgttgcg aacgcccgtt
     8941 tgccccgcaa aaaaccgtcg cactggctgc tgaattgttg gcacagcaac aaaatgcgcc
     9001 acaaaaccgc gaaatgttgc gggcgcaagc gagcgtctgc ccggagtgca aacaacgcgc
     9061 gacgctgatc aacgacgata cagatgtacc gctggtggct aaggagcagc tatgagtcca
     9121 gtgcttacac aacatgtcag ccagcccatc acgctggatg agcaaacgca aaagatgaag
     9181 cggcatttgc tacaggatat ccgtcgctca gcctacgttt atcgcgtcga ttgcggcggc
     9241 tgcaacgcct gtgaaatcga aatttttgct gccatcaccc ccgtattcga cgcagaacgt
     9301 tttggcatta aggttgtttc atcaccgcgt cacgccgata ttttgttatt tactggcgca
     9361 gtcacccggg cgatgcgtat gcctgcactt cgggcgtatg agtctgcccc cgatcataaa
     9421 atctgtgttt cctacggcgc gtgcggtgtc ggcggcggta ttttccacga tctctacagc
     9481 gtctggggcg gcagcgacac cattgtcccc attgatgttt ggatccccgg ctgcccgcca
     9541 acaccggccg ccaccattca cggtttcgcc gtggcgctcg gtttgctgca acagaagatt
     9601 cacgctgtgg attatcgcga tcccaccggg gtgactatgc aaccgttgtg gccacagatc
     9661 ccgccatcac agcgtatcgc cattgagcga gaagcgcggc ggctggcggg ctatcgtcag
     9721 gggcgagaaa tttgcgatcg gctcctgcgc catttaagcg acgatcctac aggaaatcgg
     9781 gttaacacct ggttgcgcga tgccgacgat ccacgtctca atagtatcgt tcagcaactc
     9841 tttcgcgtac tccgggggtt acatgactga agagtgcggg gaaattgttt tctggacgct
     9901 gcgaaaaaag tttgtcgcca gtagcgacga gatgccggaa caccgctctc aggtaatgta
     9961 ttactcgctg gctatcggcc atcacgttgg cgtgattgat tgtctgaatg tcgccttccg
    10021 ctgcccactg acggaaaacg aagattggct tgcacaggtc gaagaggagc aagcccgacg
    10081 taagatgctg ggggtgatga cctttggtga gattgttatt gacgccagcc acaccgccct
    10141 gttgacccgg gcattcgcgc tactggcaga tgacgcgacg tctgtgtggc aggcgcgtag
    10201 cattcaattc attcatctgt tggatgaaat tgtgcaggaa ccggccatct atctgatggc
    10261 cagaaaaatt gcgtgagaag gatttctcat taataaggac tgttgatggc tatgtcagac
    10321 gaggcgatgt ttgccccgcc acaaggaata acaattgaag cggtaaacgg aatgctcgcg
    10381 gagcggttag cacagaaaca cggtaaggcg tctttattac gcgccttcat cccgatgccg
    10441 ccgccgttca gcccggtaca acttattgaa ctgcatgtta tcaaaagcaa cttctattac
    10501 cgctaccatg atgatggcag cgatgtgacg gcaacaacag agtatcaggg cgagatggtc
    10561 gattattcgc gtcacgccgt ccttctcggc agtagtgaaa tggcggagct acgctttatt
    10621 cgtacccacg gcagccgttt tactccctag gattgcacac tgtttaactg gctggcacgc
    10681 attatcactc cggttctgca atcatggctc aatgatgaag aacaacaggt ggcgctgcgt
    10741 ttgctggaga aagatcgcga tcatcatcgg gtactggttg atattactaa tgcagtgctg
    10801 tcacatcttg atctcgacga tctgatcgct gacgtcgctc gtgagatcca tcattttttc
    10861 ggtctggctt cagtcagtat ggtactgggc gatcatcgaa agaacgagaa gtttagcctg
    10921 tggtgcagcg atctttctgc ctcacattgt gcgtgtctgc cacgcaatat gcctggcgac
    10981 agtgtattgc tgacacaaac gctacaaacc cgacaaccga ccttgacgca ccgtgcagac
    11041 gatctgtttc tctggcaacg cgacccgtta ttactcttac ttgcatctaa cggctgcgaa
    11101 tctgcgctcc ttataccgct tacctttggc aaccatacac cgggtgcatt gttgctggcg
    11161 catacctctt ccactctctt tagtgaggaa aactgccagc tactacaaca catagccgat
    11221 cgcatcgcta ttgccgttgg caatgccgat gcctggcgta gcatgaccga tttgcaggaa
    11281 agtttgcagc aagaaaacta ccagcttagc gagcagctcc tttcgaatct gggcatcggt
    11341 gacattatct atcaaagcca ggcaatggaa gacctgctcc agcaggtaga tattgtagcg
    11401 aagagcgaca gtacggtgtt gatttgtggt gaaaccggaa ccggcaaaga ggtgatcgcc
    11461 agagcgctcc atcaacttag cacgcgacgc gacaaaccgc tggtcaaaat caactgcgct
    11521 gccatccccg ccagtcttct ggaaagtgag ttattcggtc atgacaaagg ggcgtttact
    11581 ggtgcgatta atacacatcg tggtcgtttt gaaattgccg atggcggcac gttgtttctc
    11641 gatgaaattg gcgatctgcc gttagaactt cagcctaaac tgctgcgcgt attgcaggaa
    11701 cgggagattg agcgtctcgg cgggagtaga acgatcccgg tgaatgtcag agtcattgcc
    11761 gccaccaacc gtgatttgtg gcaaatggtt gaagatcgcc agtttcgcag caatctcttt
    11821 tatcgcctga atgtcttccc attggaattg ccgccgctac gcgaccgtcc ggaagatatc
    11881 cctcttttag caaaacattt cacgcaaaaa atggcgcgcc atatgaatcg cgcaattgac
    11941 gccatcccga ccgaggtgct acgccagttg atgtcgtggg actggccggg caacgtgcac
    12001 gagctggaaa acgtgattga gcgagcggta ctgctgactc gcggtaacag tctgaattta
    12061 catcttaatg tccgacaaag ccgtttactg ccgacgctaa atgaagattc agcgcttcgc
    12121 agttcaatgg cgcagttgct gcacccgacg acgccagaga atgacgaaga agaacgtcag
    12181 cgcattgttc aggtattgcg agaaaccaat ggcattgttg ccgggccccg tggcgcggcg
    12241 acacgattag ggatgaagcg caccacgctg ctgtcacgaa tgcagcgtct ggggatctcg
    12301 gttcgcgagg tgttgtaatc tgcttttgca ggagtatgca tgagaaacaa actctctttc
    12361 gacttgcagt tgagcgccag aaaagcggca atcgctgaac ggattgccgt ccataaaatt
    12421 gcccgcagta aagtgtcggt ctttttaatg gcgatgtccg ctggcgtgtt tatggcgatc
    12481 ggatttactt tttacctttc cgttatcgcc gatgccccgt cttcacaggc attaacccat
    12541 ctggtgggcg gcctttgctt tacactcggc tttattttgc tggcggtttg cggcacagcc
    12601 tgttcacctc gtcggtaatg acggtgatag caaaaagtcg gggcgttatt agttggcgaa
    12661 cttggctgat taacgcactt ctggtggcct gtggtaatct ggcaggtatt gcctgtttca
    12721 gtttgttaat ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg ggagtcgcgg
    12781 ttttacactg cgccgagggc aaaatgcatc atacatttac tgaatctgtc agcctcggca
    12841 ttatgtgcaa tctgatggtt tgcctggcgc tgtggatgag ttattgcggg cgttcgttat
    12901 gcgacaaaat cgtcgccatg attttgccca tcaccctgtt tgtcgccagt ggctttgagc
    12961 actgtatcgc caatttgttt gtgattccgt tcgccattgc cattcgccat ttcgccccct
    13021 cccttctggc aactggcgca cagtagcgca gacaattttc cggcactgac ggtcagccat
    13081 tttattaccg ccaatctgct cccggtgatg ctgggtaata ttatcggcgg tgcagtgctg
    13141 gtgagtatgt gttatcaggc tatttattta cgtcaggaat cctgaaaaat cagcccggcg
    13201 aaacagttcg tcgggctgaa gattattctt gcgcgatttg cccatcgggc caggcgtgga
    13261 tcacggcttt gatcagcgtc gccaatggaa tggcgaagaa tacgccccag aatccccaca
    13321 aaccaccgaa gatcaccacc gataaaataa tcaccagcgg atgcaggtta accgcttcgg
    13381 agaacaacac cggtactagc aggttgccgt ccagcgcctg aataatcaga tacaccgcga
    13441 agcagctcca gaattccgtg cctgcgccaa actggaatag cgccacgcca accaccggaa
    13501 tggtcaccac aaatgcgcca atgtacggaa taagaaccga gaagccgacc agcaccgcca
    13561 gcagcagcga atagttcagc ccaaagagca agaaccccag ccaggtggcg atccccacca
    13621 cgatcatctc cagcactttg ccgcggatat agttggtgat ttgttgattc atctccttcc
    13681 acacctgtcc tgccagtcca cggttgcgcg gcagcacccg acgaacggcg ttcagcatct
    13741 gctctttgtc tttcagcagg aagaagacca tcaatggcac cagcaccaga tagacggcaa
    13801 tggtcagcaa accgaccagc gaggcgaggg aaattttcac caccgaatcg cccatggtca
    13861 gcatccgact gcgcatattt tcggccattg catcaataat gcccgcgtcc attaacgccg
    13921 gatagcggcg cggcaacgtg gcggcaaagt cagaaagctt attgagcatc cccggcatat
    13981 cgcggattaa gtagatgccc tgttgccagg cgataggcag taccacgaac gccatcagta
    14041 gcaatatacc gacaaaaacc accaatacaa tcgacgtcgc ccagcggcgg gggcagccaa
    14101 tagattgcag gcgcacggtt ggccattcca gcaaataggc cagcacaata gccaccagca
    14161 acggagcaag caggccacta aagaaaaaga taatgccaaa tccggcaact aaaataacca
    14221 gcaaggcaat cgcttccggg tcgctaaaac ggcggcgata ccattgcatc aacatttcga
    14281 gcataaaaac cttccctgaa cctcaagagc gggattgcga tccgcaattg tatcgaaatg
    14341 tcacaaaaaa gacttcgctt tttatgacgg attcaggaaa ctgaaaagtc atttgagtgg
    14401 gctaatcttc gccgttacac tcaaaggcgg cgcggtggga acgatatttc acagtatcgg
    14461 tcaaatgact atcttcagaa atacaggata gaggttatgt tcaggcagtt gaaaaaaaac
    14521 ctggttgcaa ccctcattgc tgctatgacc attggtcagg tagccccggc gtttgccgac
    14581 aacgcagaca ccttgccgga tatgggaacc tccgcaggaa gcacgctttc cattggtcag
    14641 gaaatgcaga tgggcgacta ttatgtccgc cagctacgcg gcagcgcgcc gttaattaat
    14701 gacccgctgt taacgcaata tattaattcg ctggggatgc gtctggtttc gcatgccaat
    14761 tcggttaaga caccgtttca tttttttcta atcaacaacg acgaaattaa cgcctttgct
    14821 ttctttggcg gcaacgtggt gctgcactct gccctgttcc gttattccga taacgaaagt
    14881 caactggctt cagttatggc gcacgaaatc tcccacctca cccaacgtca cctggcgcga
    14941 gcgatggaag atcagcagcg cagcacgccg ctgacctggg tcggcgcgtt aggttctatt
    15001 ttactggcga tggccagtcc gcaggcgggg atggcggcgc tgaccggtac actggcggga
    15061 acgcgtcagg ggatgatcag tttcacccag caaaatgaac aggaagcgga ccgcattggt
    15121 attcaggtgc tgcaacgctc gggattcgat ccgcaggcga tgccaacctt cctcgaaaaa
    15181 ttactcgatc aggcgcgtta ctcctcgcgc ccgccggaaa ttttattgac tcacccgttg
    15241 ccggaaagtc gtctggcaga tgcccgcaac cgtgctaatc agatgcgacc gatggtggtg
    15301 cagtcgtcgg aagatttcta tctggcgaaa gcgcgcacac tggggatgta taattccgga
    15361 cgtaaccagc tcaccagtga tttgctggat gaatgggcga aaggaaacgt tcgtcagcaa
    15421 cgagcggcgc aatatggtcg tgctttacag gcgatggaag ccaataaata cgacgaggcg
    15481 cgtaaaacgc tgcaaccgtt actggcggca gaacctggca acgcatggta tctcgatctg
    15541 gctaccgata tcgatcttgg gcaaaacaaa gccaatgagg caatcaatcg cctgaaaaat
    15601 gcccgtgatt tgcgcaccaa tccggtgttg cagctcaacc tggcaaacgc ttatctgcaa
    15661 ggcggtcaac cacaagaagc ggccaatatt ctcaatcgct acacctttaa taataaagat
    15721 gacagcaacg gctgggattt actggcacag gcggaagccg cgctaaataa ccgcgatcag
    15781 gagctggctg cgcgagcaga aggttatgcg ctcgccggac gactcgatca ggccatttcg
    15841 ctgttgagta gcgccagttc gcaggtgaaa ttaggcagcc tgcaacaagc gcgttacgat
    15901 gcgcgcatcg accagttgcg ccagctgcag gaacgcttta agccttatac caagatgtaa
    15961 ttcgcattaa aggaagaaca atgaccaaac aggtaaaaat ctaccataac ccacgctgtt
    16021 caaagagccg ggaaacgctg aacctgctga aagaaaacgg cgtggagcca gaagtggtac
    16081 tctaccttga gacacccgcc gatgcggcaa cgctgcgcga tttgctgaaa atgctgggga
    16141 tgaatagcgc ccgtgaattg atgcgccaga aagaggatct ttataaagaa ctgaacctgg
    16201 ctgacagttc gcttagcgaa gaggcgctga ttcaggcaat ggtggataat ccgaagctga
    16261 tggagcgccc gattgtggtc gcgaatggca aagcgcggat tggtcgtcca ccggagcagg
    16321 tactggaaat cgtgggttaa gatgtgctcc atcgctagtt gaagcacatt gccggatgcg
    16381 acgcttgcca tgtcttatcc ggcctacgaa tactatggat taacttcgcg tagttcggat
    16441 aaggcgttcg cgccgcatcc gacaataaac accttatcta caacttcaga atttctttca
    16501 caaacggaat ggtcagctta cgttgcgcgg taatcgacgc acgatccaac tgatccaacg
    16561 tcataaatag cgtgcgcatt tctctgtcga gccgcttcag caagaaacgc cccacatctt
    16621 ccggcagttc aaaaccacgc aaacgcgcgc gtaactgtag cgcctgcaac ttatcttcat
    16681 cagaaagtgg ctgcaatttg tagatctgcc cccagtcgag tcgcgacgcg agatccggta
    16741 atcccagatt caactgccgc ggtggacgat cgccggtgat caacaaccgt gttttgcccg
    16801 attccagaat tcgattgtag agatcgaaaa tcgccatctc ccacaactca tcgcctgcaa
    16861 tacactcaat gttgtcgata caaaccagcg acaaatgctc cataccgtcg agcacttccg
    16921 gaacaaacca agtgcgttta tccagcggga catagcccac cgcatcgcca cgctgcgaca
    16981 attccgcgca agccgcgtgc agcagatggc tacgccccgc gccttcgcgt gcccagagat
    17041 agatgtaacc gctatgttcc tgacgcagca cgttttgcag cgcggccagt aaagaggagt
    17101 tatcccccgg ccagaaactt gcaaaggttt cgtcgtcagg aagataaagt ggcaaagaga
    17161 gctgtgccgg tgtgttcaga gatacctcaa ccaggatttc acaaaatcgc gagaagttta
    17221 ccacagaatc ccatgatgtt tgaaccgggc agcaacactg cccggtcggt acattatttg
    17281 tctgttatat ccgcctcttc tgcgtccagc accacttctt cagggcgcag cacactgatc
    17341 agtttgaaaa tcaaacttaa gccgataccg acgatggtcg ccagcgccat accttttaac
    17401 tccgccgcgc cgatgtttac cttcgcgcca ctgacgccga tgatcaaaat cacggaagtc
    17461 aggatcaggt tctgtgcttt gttgtaatcc actttcgatt cgatcaaaac acgaataccg
    17521 gaagcaccga tgacaccata aagcagcagc gaaacgccgc ccatcaccgg caatgggatc
    17581 atctggatag cggcagccag tttaccgacg caggaaagca ggatagcgaa aatcgccgcc
    17641 ccgccgataa cccaggtact gtaaacacgg gtgatcgcca tcacgccaat gttttctccg
    17701 taagtggtat ttggcgtgga gccaaagaag ccggagatca cggtcgacag gccgttagca
    17761 aacatcgaac ggtgcagacc tggatcgcgc agcagatctt ttttgacgat attagccgtt
    17821 actaccaggt gccctacgtg ttcggcaata accactaacg ccgctggcag aatagtcaga
    17881 atggcaaacc actcgaagcg cggcgtatag agggttggca gcgcaaacca gtgagcatta
    17941 ataatcggcg tggtatcgac aattcccatt gcgaaagaga gcgcgtagcc caccagcacg
    18001 ccaattaaaa tcgggataat tgccaggaaa ccacgaaaca gcacggaacc taaaaccgtg
    18061 accgccaggg tggtaataga gatgatgatg gttttggagt ctggcgtttg cccttcagcc
    18121 gggagtaaac ccgccatacc ggcagctacg cccgccagct ccagaccgat gacggcaacg
    18181 attgcgccca ttgccgcagg tggaaacagc acgtccagcc agccggtccc cgctttcttc
    18241 acgataaaag aaaccaggca gaacagcacg ccgcacataa taaagccgcc cagcgcgact
    18301 tcatacccta acggcaacag taacaatacc ggtgaaataa aggcaaagct ggaaccaaga
    18361 taagccggaa ttttcccttt acagatgaag agatacagca gcgttccaat accgttaaat
    18421 aacagtacag tcgccgggtt aatatgaaat aagacgggca ccaggacggt tgcaccaaac
    18481 atggcgaaca aatgttgcaa actaagcggg attgtctgta aaagtggcgg tctttcactc
    18541 accccgatag cacggcgcgt catagtatta tcctctgtat tatgtgttat aggcgcttta
    18601 ctcaaaaaaa agccgactct taaagtcggc tttaattatt tttattcttt atttcgtacc
    18661 aaagattttg tcaccggcat cgccgaggcc cggaataatg tatccgtgct cgttcagtcc
    18721 ctgatcaatc gatgcggtat acagttcgac gtccgggtgc gctttttcca gcgcagcgat
    18781 accttctggc gcagcgacca gcaccagaac tttgatgctg ctgcagcccg cttttttcag
    18841 caggtcgatg gtcgcgataa cggaaccacc ggttgccagc attgggtcaa cgatcagcgc
    18901 catacgctca tcgatgttag aaaccagttt ctggaagtac ggtaccggct ccagcgtttc
    18961 ttcattacgg tacataccga caacgctgat gcgcgcgctc ggaacatttt ccagcacacc
    19021 gtccatcata ccaagacccg cacgcagaat tggcacaacg gtaattttct tacctttgat
    19081 ctggtcgatt tctaccgggc cgttccagcc ttcgatagtt actttttccg tttcgaggtc
    19141 ggcggtcgct tcgtaagtca gcaggctacc cacttcggaa gcgagttcgc gaaagcgctt
    19201 ggtgctgata tcttgctcac gcatcagtcc cagcttgtgt ttgacgagtg ggtgtttgac
    19261 ttccacgatc ttcatactct ttctcctttg aggggcagcc acaaaaaaat cgacggatta
    19321 tacctccttt cttcaaggcg gcaatattct tttcgttgac tttagtcaaa atgataacga
    19381 tttgagataa agttatttta tattcaaatg gttatgaaag aagatcattc catccgaaaa
    19441 ctaacctttg ccctggcaca agtcttcttt cgccgcgcgt ctggggaaaa gacgtgcaaa
    19501 aaggttgtgc aaagcagcct cgcaaacgtt tgctttccct gttagaattg cgccgaattt
    19561 tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa acctctctta
    19621 gctacaaaga tgccggtgtt gatattgacg cgggtaatgc tctggttgga agaatcaaag
    19681 gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc ttcggtgcgc
    19741 tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt ttccggcact gacggcgtag
    19801 gtaccaagct gcgtctggca atggacttaa aacgtcacga caccattggt attgatctgg
    19861 tcgccatgtg cgttaatgac ctggtggtgc aaggtgcaga accgctgttt ttcctcgact
    19921 attacgcaac cggaaaactg gatgttgata ccgcttcagc ggtgatcagc ggcatcgcgg
    19981 aaggttgtct gcaatcgggc tgttcactgg tgggtggcga aacggcagaa atgccgggga
    20041 tgtatcacgg tgaagattac gatgtcgcgg gtttctgcgt gggcgtggta gaaaaatcag
    20101 aaatcatcga cggctctaaa gtcagcgacg gcgatgttct gattgcactc ggttccagcg
    20161 gtccgcactc gaacggctat tcgctggtgc gcaaaattct tgaagtcagc ggttgtgatc
    20221 cgcaaaccac cgaacttgat ggtaagccat tagccgatca tctgctggca ccgacccgca
    20281 tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc attgcgcacc
    20341 tgaccggcgg cggcttctgg gaaaacattc cacgcgtatt gccagataat actcaggcag
    20401 tgattgatga atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa acggcaggta
    20461 acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg cgtcgggatg attattgccc
    20521 tgcctgctcc ggaagtggac aaagccctcg ccctgctcaa tgccaacggt gaaaacgcgt
    20581 ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc gaataatgaa
    20641 tattgtggtg cttatttccg gtaacggaag taatttacag gcaattattg acgcctgtaa
    20701 aaccaacaaa attaaaggca ccgtacgggc agttttcagc aataaggccg acgcgttcgg
    20761 ccttgaacgc gcccgccagg cgggtattgc aacgcatacg ctcatcgcca gcgcgtttga
    20821 cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc gacatgtacg cacccgatgt
    20881 ggtcgtgctg gctggtttta tgcgcattct cagcccggcg tttgtctccc actatgccgg
    20941 gcgtttgctg aacattcacc cttctctgct gccgaaatat cccggattac acacccatcg
    21001 tcaggcgctg gaaaatggcg atgaagagca cggtacatcg gtacatttcg tcaccgatga
    21061 actggacggt ggcccggtta ttttacaggc gaaagtcccg gtatttgctg gtgatacgga
    21121 agatgacgtc accgcccgcg tgcaaaccca ggaacacgct atttatccac tggtgattag
    21181 ctggtttgcc gatggtcgtc tgaaaataca cgaaaacgcc gcgtggctgg atggtcaacg
    21241 tctgccgccg cagggctacg ctgccgacga gtaatgcccc cgtagttaaa gcgccagctc
    21301 tgccgctggc gtttttcaat tcacctgtaa atcgcaagct ccagcagttt ttttccccct
    21361 tttctggcat agttggacat ctgccaatat tgctcgccat aatatccagg cagtgtcccg
    21421 tgaataaaac ggagtaaaag tggtaatggg tcaggaaaag ctatacatcg aaaaagagct
    21481 cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg gcggacaaat ctaacccgct
    21541 gattgaaagg atgcgtttcc tggggatcta ttccaataac cttgatgagt tctataaagt
    21601 ccgcttcgct gaactgaagc gacgcatcat tattagcgaa gaacaaggct ccaactctca
    21661 ttcccgccat ttactgggca aaattcagtc ccgggtgctg aaagccgatc aggaattcga
    21721 cggcctctac aacgagctat tgctggagat ggcgcgcaac cagatcttcc tgattaatga
    21781 acgccagctc tccgtcaatc aacaaaactg gcttcgtcat tattttaagc agtatctgcg
    21841 tcagcacatt acgccgattt taatcaatcc tgacactgac ttagtgcagt tcctgaagga
    21901 tgattacacc tatctggcgg tggaaattat ccgtggagac accatccgtt acgcgctgct
    21961 ggagatccca tcagataaag tgccgcgctt tgtgaattta ccgccagaag cgccgcgtcg
    22021 acgcaagccg atgattcttc tggataacat tctgcgttac tgccttgatg atattttcaa
    22081 aggcttcttt gattatgacg cgctgaatgc ctattcaatg aagatgaccc gcgatgccga
    22141 atacgattta gtgcatgaga tggaagccag cctgatggag ttgatgtctt ccagtctcaa
    22201 gcagcgttta actgctgagc cggtgcgttt tgtttatcag cgcgatatgc ccaatgcgct
    22261 ggttgaagtg ttacgcgaaa aactgactat ttcccgctac gactccatcg tccccggcgg
    22321 tcgttatcat aattttaaag actttattaa tttccccaat gtcggcaaag ccaatctggt
    22381 gaacaaacca ctgccgcgtt tacgccacat ttggtttgat aaagcccagt tccgcaatgg
    22441 ttttgatgcc attcgcgaac gcgatgtgtt gctctattat ccttatcaca cctttgagca
    22501 tgtgctggaa ctgctgcgtc aggcttcgtt cgacccgagc gtactggcga ttaaaattaa
    22561 catttaccgc gtggcgaaag attcacgcat catcgactcg atgatccacg ccgcacataa
    22621 cggtaagaaa gtcactgtgg tggttgagtt acaggcgcgt ttcgacgaag aagccaacat
    22681 tcactgggcg aagcgcctga ccgaagcagg cgtgcacgtt atcttctctg cgccggggct
    22741 gaaaattcac gccaaactgt tcctgatttc acgtaaagaa aacggtgaag tggtccgtta
    22801 cgcacacatc gggaccggga actttaacga aaaaaccgcg cgtctttata ctgactattc
    22861 gttgctgacc gcagatgcgc gtatcaccaa cgaagtacgg cgggtattta actttattga
    22921 aaacccatac cgcccagtga catttgatta tttaatggtg tcaccgcaaa actctcgccg
    22981 tctgttatat gaaatggtag accgcgaaat cgccaacgcg cagcaagggc tgcccagtgg
    23041 tatcaccctg aagctaaata accttgtcga taaaggcctg gttgatcgtc tgtatgcggc
    23101 ctccagctcc ggcgtaccgg ttaatctgct ggttcgcgga atgtgttcgc tgatccccaa
    23161 tccggaaggc attagcgaca acattcgtgc catcagtatt gttgaccgtt accttgaaca
    23221 tgaccgggtt tatatttttg aaaatggcgg cgataaaaag gtctaccttt cttccgccga
    23281 ctggatgacg cgcaatattg attatcgtat tgaagtggcg acaccgctgc tcgatccgca
    23341 cctgaagcag cgggtgctgg acatcatcga catattgttc agcgatacgg tcaaagcacg
    23401 ttatatcgat aaagaactca gtaatcgcta cgttccccgc ggcaatcgcc gtaaagtacg
    23461 ggcgcagttg gcgatttacg actacatcaa atcactcgaa caacctgaat aaccctatgc
    23521 caatacacga taaatcccct cgtccgcagg agtttgctgc ggtcgatctt ggttcaaaca
    23581 gttttcacat ggtcatagcc cgtgtggtag atggtgccat gcagattatt ggccgcctga
    23641 aacagcgagt gcatctggcg gacggcctgg ggccagataa tatgttgagt gaagaggcaa
    23701 tgacgcgcgg tttgaactgt ctgtcgctgt ttgccgaacg gctacaaggg ttttctcctg
    23761 ccagcgtctg tatagtcggc acccatacgc tgcgtcaggc gctgaacgcc actgactttc
    23821 tgaaacgcgt ggaaaaggtc attccctacc cgattgaaat tatttccggt aatgaagaag
    23881 cccgtctgat ttttatgggc gtggaacata cccaaccgga gaaaggtcgc aaactggtta
    23941 ttgatattgg cggcggatct acggaactgg tgattggtga aaatttcgaa ccgattctcg
    24001 ttgaaagccg ccggatgggt tgtgtcagct ttgcccagct ttacttccct ggcggggtca
    24061 tcaataaaga gaattttcag cgcgctcgta tggcggcagc acaaaaactg gaaactttaa
    24121 cctggcaatt ccgtattcag ggctggaacg tggcaatggg cgcttccggt accataaaag
    24181 ccgcccatga agtgttaatg gaaatgggcg agaaagacgg gataattacc ccggaacgtc
    24241 tggaaaaact ggtaaaagaa gttttacgtc accgtaattt cgcatcgctg agtttaccgg
    24301 gtctttccga agagcggaaa acagtcttcg ttcctggact ggcgatttta tgcggtgtgt
    24361 ttgatgcttt agccatccgt gaactgcgcc tttctgacgg ggcgttacgc gaaggcgtac
    24421 tgtacgaaat ggaaggacgt ttccgtcatc aggatgtgcg tagtcgcacc gccagcagcc
    24481 tcgccaacca gtatcacatc gacagcgaac aggcccgacg ggtgctggat accactatgc
    24541 aaatgtacga acagtggcgg gaacagcaac cgaagctggc gcatccgcaa ctggaggcgc
    24601 tactgcgatg ggccgccatg ctgcatgagg tcgggttgaa tatcaaccac agcggtttgc
    24661 atcgccactc cgcttatatt ctgcaaaaca gtgacttgcc gggttttaat caggaacagc
    24721 agctgatgat ggcgacactg gtgcgctatc accgcaaagc gattaaactc gacgatctgc
    24781 cgcgctttac cttgtttaag aagaaacagt tcctgccact gattcagcta ttgcgccttg
    24841 gcgtattact caacaatcaa cgtcaggcaa ccaccacacc gccaacattg acactgatta
    24901 ctgatgacag tcactggaca ctgcgtttcc cgcatgactg gtttagtcag aatgcgttgg
    24961 tactgcttga tctggaaaag gagcaagaat actgggaagg cgtggctggc tggcggttga
    25021 aaattgaaga agaaagtaca cctgaaatcg ccgcttaatg ctttgcgggc cgacgagaaa
    25081 tgtcggcccg cattattcag gcactttcgc gaatgggtt
//
LOCUS       DADLTD010000039        24875 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLTD010000039 DADLTD010000000
VERSION     DADLTD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.039"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..337)
                     /locus_tag="JN701_001283"
     CDS             complement(<1..337)
                     /locus_tag="JN701_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363791.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAA"
     gene            complement(453..1004)
                     /gene="maa"
                     /locus_tag="JN701_001284"
     CDS             complement(453..1004)
                     /gene="maa"
                     /locus_tag="JN701_001284"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY7363792.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1176..1394)
                     /gene="hha"
                     /locus_tag="JN701_001285"
     CDS             complement(1176..1394)
                     /gene="hha"
                     /locus_tag="JN701_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7363793.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1420..1794)
                     /gene="tomB"
                     /locus_tag="JN701_001286"
     CDS             complement(1420..1794)
                     /gene="tomB"
                     /locus_tag="JN701_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY7363794.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2340..5489)
                     /locus_tag="JN701_001287"
     CDS             complement(2340..5489)
                     /locus_tag="JN701_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY7363795.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5512..6705)
                     /gene="acrA"
                     /locus_tag="JN701_001288"
     CDS             complement(5512..6705)
                     /gene="acrA"
                     /locus_tag="JN701_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY7363796.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6847..7494
                     /gene="acrR"
                     /locus_tag="JN701_001289"
     CDS             6847..7494
                     /gene="acrR"
                     /locus_tag="JN701_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY7363797.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7622..10984
                     /gene="mscK"
                     /locus_tag="JN701_001290"
     CDS             7622..10984
                     /gene="mscK"
                     /locus_tag="JN701_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY7363798.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11196..11357)
                     /gene="rsmS"
                     /locus_tag="JN701_001291"
     CDS             complement(11196..11357)
                     /gene="rsmS"
                     /locus_tag="JN701_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY7363799.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11371..11898)
                     /gene="priC"
                     /locus_tag="JN701_001292"
     CDS             complement(11371..11898)
                     /gene="priC"
                     /locus_tag="JN701_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY7363800.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11968..12345
                     /locus_tag="JN701_001293"
     CDS             11968..12345
                     /locus_tag="JN701_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY7363801.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12498..13049
                     /gene="apt"
                     /locus_tag="JN701_001294"
     CDS             12498..13049
                     /gene="apt"
                     /locus_tag="JN701_001294"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY7363802.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13178..15049
                     /gene="dnaX"
                     /locus_tag="JN701_001295"
     CDS             13178..15049
                     /gene="dnaX"
                     /locus_tag="JN701_001295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY7363803.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     gene            15102..15431
                     /locus_tag="JN701_001296"
     CDS             15102..15431
                     /locus_tag="JN701_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY7363804.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15431..16036
                     /gene="recR"
                     /locus_tag="JN701_001297"
     CDS             15431..16036
                     /gene="recR"
                     /locus_tag="JN701_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY7363805.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16146..18020
                     /gene="htpG"
                     /locus_tag="JN701_001298"
     CDS             16146..18020
                     /gene="htpG"
                     /locus_tag="JN701_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY7363806.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18201..18845
                     /gene="adk"
                     /locus_tag="JN701_001299"
     CDS             18201..18845
                     /gene="adk"
                     /locus_tag="JN701_001299"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY7363807.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            18977..19939
                     /gene="hemH"
                     /locus_tag="JN701_001300"
     CDS             18977..19939
                     /gene="hemH"
                     /locus_tag="JN701_001300"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY7363808.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19936..20895)
                     /gene="aes"
                     /locus_tag="JN701_001301"
     CDS             complement(19936..20895)
                     /gene="aes"
                     /locus_tag="JN701_001301"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAY7363809.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21047..22351
                     /locus_tag="JN701_001302"
     CDS             21047..22351
                     /locus_tag="JN701_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY7363810.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22484..24160)
                     /locus_tag="JN701_001303"
     CDS             complement(22484..24160)
                     /locus_tag="JN701_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY7363811.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24398..>24875)
                     /locus_tag="JN701_001304"
     CDS             complement(24398..>24875)
                     /locus_tag="JN701_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7363812.1"
                     /translation="YYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGR
                     KYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGM
                     VSGLFFGFAFGMGGLGAAVLGLIAAHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6216 a         6483 c         6390 g         5786 t
ORIGIN      
        1 gcgcggcagg tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg cggaaatcca
       61 gcacgtcgat atcttcatag cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa
      121 gcaggataaa taatacaacc agtagccaga cagagcctga cagcgtttcg gactgcaaca
      181 tcaccgccag ggtggcaaaa aaagcaacgt acataccgat aaacagcccc ggatgtttac
      241 ggataaaact gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact
      301 caatcgtttc ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca
      361 actttccagg gaacacccta ttttaacgtg ggaatagtag aggggaagta acagatctac
      421 cacaattttg cataacagtt gcgaaaaacc gattacaatt ttttaattat tctggctgga
      481 ttaccgccca cgacaacgtt gtccgggaca ccttttgtga caactgcacc tgaagctacc
      541 acgacgttat caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc aatccagacg
      601 ttattaccga tggtgacggg tttccccagt tcagcaccgc tattacgtgc tacagggtcg
      661 atgggatgtg ttgccgtgta gatatgaacg cctggtgcca acatacagtt atcaccgatg
      721 cgaatagggc agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt attgccgaga
      781 aaaatgttat agccataatc acagcgaaac gttggctcaa tataagcctc tgtcacctga
      841 ccgaataaat cagcgagaat ttgctggcgt aatgtgtgct cttccgccag ggaatgattg
      901 tatcggtgaa taagctgacg agcgcgcagg cgatcgcgag ataacgtctc atctgccgag
      961 cgatacaact caccagcaat catcttttct ttttctgtgc tcattactga cctccggttg
     1021 ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa cagaacgttc ccattacatc
     1081 tgtgtgataa agatcacata gggtatgtct tcatggtgaa aaagtataaa attcttaata
     1141 aacagccggt tatagctccg aaagcgaatt atttattagc gaataaattt ccatactgag
     1201 gaagggatct tgtcgtatag tttattcatg gtcaattcgg cgaggcggtg atctgcggct
     1261 gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc gataacacgc
     1321 tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt tttcgttaaa
     1381 ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata aactcgcagg
     1441 attctctttc gtcgcattga caaaacaacg gaatagtcga ttacctgact tccgccattt
     1501 ctgaagatcc tgcatattaa taccataact actgaacaac ataaaggtgt catccagata
     1561 ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt aattaagtgc
     1621 gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg aggttgggtc
     1681 gttaacccag ccataattgc tttcttcaag gtttgcaagg cagtcatgat acagggtttc
     1741 acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat ccataacgcg
     1801 tccccttctt agcggttgaa ctaacggaca cctttcggga tggaaaaaac ttactgacct
     1861 ggacttgccc tctcgtttgt tagcttaact ataagccact ctttgcaggt attcatcgca
     1921 ttattacgaa aaattacaat taggaaggaa tcgggacagg actcactttt tcatactagc
     1981 actaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt caggattatc
     2041 gccagatgaa attggtgaca ctaataccag gattgctctg aatatgacgt aataaccgag
     2101 gaatgaataa agaattaccg caaataatta agaatagcct ctaaatgatt atggattatt
     2161 aaatctatta gcgctactta atataaatta actaacaaac agtatttttt atgaatttta
     2221 tccgtggtta atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa
     2281 tgttcgtagg ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat
     2341 caatgatgat cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg
     2401 cgaaccacca caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc
     2461 ccgcccatta caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata
     2521 accagcggca taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc
     2581 atccgcactg catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct
     2641 ttggcgaatt cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg
     2701 cctacttgga agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg
     2761 ataaccccca gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc
     2821 gccgctagac acaggaatac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg
     2881 ccggagaggc gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt
     2941 ttgctcgcca gttgttccat tagctccatt gcttcaccgg tacttttacc cggtgccgcc
     3001 tggcctaaga tttccatgga tggcaggcca ttgtaacgtt ccagacgcgg cgaaccgtac
     3061 tcccaacgag aagaggagaa tgccgagaac ggcaccatct gaccatcagc agcacgaaca
     3121 taccagtcgc cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact
     3181 ttcttcacac gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc
     3241 agagtggtgt taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata
     3301 tcaatcttaa actgcggggt atcttccaga ccgtttggac gtacgctggt caacatatca
     3361 ggatgctttg ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt ttcgtgacca
     3421 aggccagcct ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt
     3481 gcgggcaggt taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca
     3541 cgcatggtaa tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag
     3601 gaaacgaacg caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg
     3661 aacaccgact caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc
     3721 actttctgcg tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg
     3781 ccctggtcct catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg
     3841 accacgatga tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg
     3901 ctgtcggtgt agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct
     3961 tttttacctt ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc
     4021 ggagtcagga tcaacgctac cagtaccgac agcgccattg ctgaaacaat ggtaatagag
     4081 aactgacgat agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgct
     4141 gacagtacca tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta
     4201 gcttcttttg gcggcaaacc ttcttccgcc ataacacgct ccacgttttc taccacaacg
     4261 atagcgtcat ccaccaacaa gccgatggcg agcaccatcc cgaacattgt tagcgtgttt
     4321 atcgagaagc caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca
     4381 atggtcggaa tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg
     4441 aggatgatcg cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc
     4501 gtggtgtcgt acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc
     4561 agttcagcac ggattgccgc agcggtatcc agcgcatttg caccggtcgc cagcttgatc
     4621 cccagaccgg aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc
     4681 agctcaatct tcgccacatc acgcagcagg acgcgggaac catcctgatt cactttcagc
     4741 aggattttgc cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta
     4801 agctgttggc ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc
     4861 tgcgctttga tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc
     4921 gggttcatcc agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc
     4981 gacgtacggc tgatggcatc tttcatattc gccgctacgt agtcagagat atcctcctgc
     5041 gtcatggtgc catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc
     5101 tcaacgctca ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt
     5161 ttgttctgca cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg
     5221 atctgcacgg tccccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg
     5281 ttcatattct gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg
     5341 gggtaggagg cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc
     5401 agtttgagga tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa
     5461 atcgggcgat cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt
     5521 ggactgttca ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg
     5581 tgcttttacc tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc
     5641 tgctttcaga ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg
     5701 acgggtttcc actttgtcat ccgcgccaac taccagtacg gtggcatcac cacgcggcgt
     5761 acgggttacg ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg
     5821 tgcgcgcacg aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag
     5881 ggtgatagac ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg
     5941 cgggaactta atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt
     6001 cagcgtgcca ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac
     6061 atcaacgtag atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg
     6121 taccaatgcg ccttccgtca cgttcgactt accaatgcga ccgctaatcg gagcggtgac
     6181 tttggtgtaa gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc
     6241 agcattcgcc tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg
     6301 agtaccgagc agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg
     6361 ggctttcgcc agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat
     6421 ctgatagaga gagacaccag cttcgatgtc gctaccttct ttgaaattac gcttcaggat
     6481 aatcccgcta acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag
     6541 ctcggttgtg atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg
     6601 ctggccacct tgttgggcct gtttgttgtc acatcctgtt agggctaagc tgcctgagag
     6661 catcagaacg accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt
     6721 gtccgatttc aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta
     6781 tcgtcgtgct atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac
     6841 gaacatatgg cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat
     6901 gtggctctac gtcttttctc acagcagggg gtatcatcca cctcgctggg tgagattgca
     6961 aaagcagctg gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg
     7021 ttcagtgaga tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag
     7081 gcaaaattcc ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa
     7141 tccacggtga cagaagaacg acgtcgatta ttgatggaga ttatattcca caaatgcgaa
     7201 tttgtcggag aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat
     7261 gaccgtatag aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta
     7321 atgacgcgtc gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg
     7381 ctctttgccc cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta
     7441 ctggagatgt atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga
     7501 atctgactcc aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg
     7561 tgatattctt gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat
     7621 catgactatg ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc
     7681 ttttgttttt gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga
     7741 tctgccgaca aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct
     7801 ttctgctcag gacaaactgg agctgcagga tctgacagat acattagcca ccctcgataa
     7861 aatcgatcgc ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga
     7921 aaaaatgcgc caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga
     7981 aacgcgcaaa attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc
     8041 gctggacgat ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc
     8101 gttacagacg cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca
     8161 acaaattcgc agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca
     8221 gaaagtgtta atgcaggctc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa
     8281 aagcctggaa gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac
     8341 ggcgaacagc gcccgtctgg agcaccagtt gcagctgttg caagaagcgg taaacagcaa
     8401 gcgcctgact ttaactgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg
     8461 tattcaggct aatccgctgg tgaaacagga actggaaatt aaccagcagt taagtcagcg
     8521 tctgattacc gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa
     8581 ctggctggag cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa
     8641 gggcagcctg ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga
     8701 tgaactggaa aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa
     8761 ccagcagcgt gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca
     8821 caccaacgaa gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg
     8881 cgaattgctg gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct
     8941 gcaaatcaac cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca tcctgactca
     9001 gcaaatcttt tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc
     9061 gcaaagcctg aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg
     9121 gcctgccgtt tttatcgctt tcctcgccgg tttgccgctg ctgttgattg tcgggctgat
     9181 ccactggcgt ctgggctggc tgaaagcgta tcaacaaaaa ctggcttccg ctgtgggttc
     9241 cctgcgtaac gatagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgcgc
     9301 gctgccggtg tgcctgatta ttctcgcggt tggcttgatt ctgttgacca tgcagctcaa
     9361 catcagcgaa ctgctatggt cgttcagcaa aaaactggcg attttctggc tggtgtttgg
     9421 cctgtgctgg aaggtactgg agaagaacgg cgttgccgtg cgtcacttcg gcatgccgga
     9481 acagcagacc agccactggc gtcggcaaat tgtccgcatc agtctcgcat tgctgcctat
     9541 ccatttctgg tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg
     9601 gcaagcgatg attttcttca acctgctgct gattgccttc ctggtatggc caatgtgccg
     9661 cgaaagctgg cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc
     9721 gattatcccg attgcattga tggtgctgac tgccacgggt tatttctaca ccacgctgcg
     9781 cctctccggg cgttggattg aaaccgttta tctggtgatc atctggaact tgctctacca
     9841 gacggtattg cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc
     9901 gcgtcggcag aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac
     9961 cattgcactg gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc
    10021 gctgttcggt gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatat
    10081 cgacagcatt acgctctggc attacaacgg tactgaagct ggcgctgcgg tggtgaaaaa
    10141 cgtcaccatg ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat
    10201 tcgcaacctg cctggtttgc tggaagtgct ggtgctctcg cgactgaata tgcgccaggg
    10261 cgcgtcgtat gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac
    10321 ggtgttcgga tcgctgggcg tctcttggga taaactccag tggctggcgg cagcattatc
    10381 cgtaggtctt ggttttggtt tacaagaaat tttcggtaac ttcgtctccg gtttgatcat
    10441 tctgttcgaa cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac
    10501 ggtaagtaag atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat
    10561 catcccgaac aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac
    10621 tacgcgtctg gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa
    10681 agtgttactg aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt
    10741 cttctttacg gcgtttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga
    10801 actgcgtgac cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg
    10861 tgaaaacgac atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg
    10921 cgatgaggtg acggaagtaa aacgcgacta caaaggcgat gacccgacgc cagcggtagg
    10981 gtaataacca aagggggcga catttagttg ccccgagatt actgacaaag tgcgcgttgt
    11041 tcatgccgga tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatgta
    11101 ttgcaggagc ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccac
    11161 aatcttaggg gacatttagc ggccccattt atttctcact tttccgcctc atcatcgcgc
    11221 gttaatttct tttcataatc gcgttttaca atatccagcg cgcgcagcac ggtgcgggct
    11281 gggatctgat tttcttccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg
    11341 gcattttcca gtgacatact ctctccattg ctagcgggtt aagcgcgcta gcctgttttc
    11401 gattttttcc agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg
    11461 atgcagcgtt tgctgttcca cgagatcggt cacccgcgcc aggcgggctc tgcgttcggc
    11521 aaccatctca cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg
    11581 ggcaattttc ggtggcgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc
    11641 gatggcttcc agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg
    11701 ctgctcaact gcatgacgaa gcgcagccag attatcgcca gcctcatcga gacaagcctg
    11761 cagtgttgtc gtacgggttt gaaaaaggtg tctgtcaaaa cgggcactta gcgtggcaaa
    11821 ttgcgctacc ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag
    11881 cagcagggcg gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag
    11941 ccactctgta agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg
    12001 ctggcggtag tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt
    12061 atcctgctgg cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg
    12121 taccgctcat ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc
    12181 ggcgtcaaac cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc
    12241 gtccagatgc catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat
    12301 atgtggcgaa ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca
    12361 gttgcaatta ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt
    12421 caaaggttaa acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa
    12481 caagcaggca tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca
    12541 tcaaaagcat tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac
    12601 tggaagaccc gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg
    12661 cgggcattac caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag
    12721 ctctgggtct gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa
    12781 ccatcagtga aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg
    12841 ccatcaaacc gggcgacaaa gttctggtgg tggacgatct gctggcaacc ggcggcacta
    12901 tcgaagcgac cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca
    12961 ttatcaacct gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct
    13021 acagccttgt cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg
    13081 gacagcacaa gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc
    13141 ttcgtgaatc caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg
    13201 aaaatggcgc ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact
    13261 ggcgaacggc ttgtcgttag ggcgtattca tcatgcttat cttttttccg gtacccgtgg
    13321 cgtcggaaaa acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat
    13381 taccgcgacg ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt
    13441 cgatctgatt gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct
    13501 ggataacgtc cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga tcgacgaagt
    13561 gcatatgctg tcgcgccaca gctttaacgc actgttaaaa acccttgaag agccgccgga
    13621 gcacgttaag tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc
    13681 gcgctgtttg caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcaacttga
    13741 gcacatcctc aacgaagaac atatcgctca cgagccgcgg gcgttgcaat tgctggcgcg
    13801 cgccgctgaa ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg
    13861 tgacggccag gtttcaaccc aggcagtcag tgcgatgctg ggtacgcttg atgacgatca
    13921 ggcgctgtcg ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat
    13981 taatgaagcc gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct
    14041 gttgcatcgt attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc
    14101 cattgagctg cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta
    14161 ctatcagacg ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gccgcatggg
    14221 cgttgagatg acgctgctgc gcgcgctggc gttccatcca cgtatgcctc tgcctgagcc
    14281 agaagtgccg cgccagtcct ttgcacccgt cgcgccaacg gcagtaatga cgccaaccca
    14341 ggtgccgccg caaccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac
    14401 caccagccag gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc
    14461 aaaaaagagt gaaccggcag ccgctacccg cgcgcgtccg gtgccatcgg cgctggaaaa
    14521 agcgccagcc aaaaaagaag cgtatcgctg gaaggcgacc actccggtga tgcagcaaaa
    14581 agaagtggtc gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga
    14641 actggcggcg aagctagcgg cagaagccat tgagcgcgac ccgtgggcgg cacgggtgag
    14701 tcaactttcg ctaccaaaac tggtcgaaca ggtggcctta aatgcctgga aagaggagag
    14761 cgacaacgca gtatgtctgc atttgcgctc ctctcagcgg catttgaaca accgcggtgc
    14821 acagcaaaaa ctggctgaag cgttgagcac gttaaaaggt tcaacggttg aactgactat
    14881 cgttgaagat gataatcccg cggtgcgtac gccgctggaa tggcgtcagg cgatatacga
    14941 agaaaaactt gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg
    15001 tcggttcttc gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca
    15061 gcttacgttc gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc
    15121 tgggtaacct gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag
    15181 agatcgcgca gctggaagtc accggcgaat ctggcgcagg tctggtaaaa gtgaccatca
    15241 acggtgcaca caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag
    15301 agatgctgga agacctggtg gctgcagcat tcaacgacgc agcacgtcgt attgaagaaa
    15361 cgcagaaaga aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga
    15421 tgccgttctg atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct
    15481 gtcgggcgtt ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg
    15541 tagcggcggg atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg
    15601 cgccgattgc cgcactttca ccgaacagga agtctgtaac atctgttcga atccgcgtcg
    15661 tcaggaaaac ggtcaaatct gtgtggtgga gagtccggcg gacatctacg ccattgagca
    15721 gacggggcag ttttctggtc gttattttgt gttgatgggg catctgtcac cgctggacgg
    15781 catcggtccg gatgatatcg ggcttgatcg tctggaacag cgtctggcag aggaaaaaat
    15841 cactgaagtg atcctcgcca ccaatcccac ggttgaaggt gaagctaccg ctaactacat
    15901 tgccgagctt tgcgcgcaat atgacgtgga agctagccga atcgctcatg gcgtaccggt
    15961 tggcggcgaa ctggaaatgg tcgatggcac cacgctgtca cactcccttg ccgggcgtca
    16021 taagattcgt ttttaagcaa acgagagcag gatcacctgc tctcgcttga aattattctc
    16081 ccttgtcccc atctctccct catcctgttt ttaaccttaa aatggcatta ttgaggtaga
    16141 cctacatgaa aggacaagaa actcgtggtt ttcagtcaga agtgaaacag cttctgcacc
    16201 tgatgatcca ttctctctat tccaataaag aaatcttcct gcgtgagctt atctctaacg
    16261 cctccgatgc ggcggacaag ctgcgtttcc gtgcgctatc taacccggac ctgtacgaag
    16321 gtgatggcga actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg ctgaccatct
    16381 ccgataacgg cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta
    16441 aatccggtac caaatcattc ctcgaatccc tgggttctga ccaggcgaaa gacagccagc
    16501 tgatcggtca gtttggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgactg
    16561 tgcgtactcg cgcggcaggc gaaaaaccag aaaacggcgt cttctgggaa tcggctggcg
    16621 aaggtgaata taccgttgcc gacatcacca aagaagatcg tggtactgaa atcaccctgc
    16681 atctgcgtga aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcatcagca
    16741 aatactccga ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagacggcg
    16801 aaaccgttat ctcctgggag aaaatcaaca aagcgcaggc gctgtggact cgtaacaagt
    16861 cggaaatcac cgatgaagag tacaaagagt tctacaaaca catcgcccac gactttaatg
    16921 atccgctgac ctggagccac aaccgcgttg aaggtaagca ggagtacacc agcctgctgt
    16981 acatcccgtc ccaggctccg tgggatatgt ggaaccgtga tcataaacac ggcctgaaac
    17041 tgtacgtcca gcgtgtgttc atcatggacg acgcagaaca gttcatgccg aactatctgc
    17101 gcttcgtgcg tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc
    17161 tccaggacag cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa
    17221 tgctggaaaa actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg
    17281 gcctggtact gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac
    17341 tgctgcgttt tgcttctacc cataccgatt cttctgcgca gaccgtatct ctggaagact
    17401 acgtttcccg catgaaagaa gggcaggaga aaatctacta catcaccgca gacagctatg
    17461 cggcagcgaa gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc
    17521 tgctttccga ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac
    17581 cgttccagtc ggtgtctaaa gttgacgagt cgctggaaaa actggctgac gaagttgatg
    17641 agagcgcgaa agaagcggag aaagcactga ctccgttcat cgaccgtgtg aaagccttgc
    17701 tcggcgagcg cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg
    17761 tttcgaccga cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc
    17821 agaaagtgcc agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac
    17881 gtgcggcaga tactgaagat gaagcgaagt tcagcgagtg ggtagaactg ctgctggatc
    17941 aggcgctgct ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga
    18001 accagctgct ggtttcctga tgtcatgccg gatgaccttt gtgttatccg gcatttttct
    18061 tttcatcatc tgcactttcc gcaaattatc tcgccattaa ccgtttcagc ctcaggtgcc
    18121 tttcttgagg caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac
    18181 attttaaggg gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag
    18241 ggactcaggc tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata
    18301 tgctgcgtgc tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg
    18361 atgctggcaa actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc
    18421 aggaagactg ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag
    18481 acgcgatgaa agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg
    18541 aactgattgt tgatcgtatc gtaggccgcc gcgttcatgc gccgtctggt cgtgtttatc
    18601 acgttaaatt caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga
    18661 ctacccgtaa agacgatcag gaagaaaccg tacgtaaacg tctggttgaa taccatcaga
    18721 tgactgcacc gctgatcggc tactactcca aagaagcgga agcgggtaac accaaatacg
    18781 cgaaagttga cggcaccaag ccggttgctg aagttcgcgc tgatctggaa aaaatcctcg
    18841 gctaatcaag ccgcagcaga tctgctttcg ggcaggtctg ctcatccacc tcaggcaata
    18901 cctctcacag caattagttc ttcttcctca cttttccgct acaattatca acaagttgaa
    18961 tcgataagag gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctgggtac
    19021 gcccgatgcc cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag
    19081 acgcgtggtt gatacctcac ggttgttatg gtggccattg ctgcgcggcg ttattttgcc
    19141 gctgcgctcg ccgcgtgtgg cgaagctgta tgcctctgtc tggatggaag gtggctcgcc
    19201 gctgatggtt tacagccgcc agcaacagca ggcgctggca caacgtttac cggagacgcc
    19261 cgtagcgctg ggaatgagct acggctcgcc atcactggaa agcgccgtcg atgaactcct
    19321 ggcagagcat gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttctac
    19381 ggtcggtgcg gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg
    19441 gatatcgttt attcgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag
    19501 cgtacgcgct tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg
    19561 cattccccag cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacgac
    19621 tcgcgaactg gcttccgcac tggggatggc accggaaaaa gtgatgatga cctttcagtc
    19681 gcgctttggc cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg
    19741 agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg attgtctgga
    19801 gacgctggaa gagattgccg agcaaaaccg tgaggtcttc ctcggtgccg gcgggaaaaa
    19861 atatgaatat attccagcgc ttaatgccac gccagaatat atcgaaatga tggctaatct
    19921 tgttgccgcg tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc gaagagcctc
    19981 gtcggcggtt ttcatcattc gtgaataatg caaaaaggca tgcagcgtac ctgggtagag
    20041 tttgaactca cagggctgct gatgcgccgc taacgtctgg taaagcagac ggctgtcatc
    20101 cagcagcgga tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc
    20161 attattaaac agacagtaat acggtgactc gcggtccgcg tcgttgctta aatacgcctc
    20221 ttcatacatc tgcaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg
    20281 agtcacggaa tcccgtaatc cgtaaagccc ataccacagc aaaacaccca caattttacc
    20341 gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg agcgccagca tggcacctgc
    20401 ggaatcaccg gcaaagccaa tgcgggacat attgatttga taatcctccg cctgctggtg
    20461 gaaataacaa caagcagcca caatttcctc tatcgcttgc ggaaaacgcg cttcaggtga
    20521 aagggggtaa tcaataccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat
    20581 gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat aaaatagcgt
    20641 cgccgggcta tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg
    20701 aaccatgtaa gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag
    20761 cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg gtaaatccgg
    20821 ctgaagagta ttcacaacgg tcttcatttc agcagaaata aggtccagaa caggtagttt
    20881 gttttccggc ttcatacaaa aactcctttc aaattacgtc attgtaagga accactgcca
    20941 tgaaaatgcg atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag
    21001 attgtgttaa cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg
    21061 taaacgtaaa tccaaacatt acttccctgt aaatgcacgc gatccgctgc ttcagcaatt
    21121 ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga cgctggtcga
    21181 tattgaagcg aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc
    21241 actggtgatt gaggatgacg tagccgaagc gctttatcag gagctaaaac agaaaaacct
    21301 gattacccat cagtttgcgg gtggcaccat tggtaacacc atgcacaact actcggtgct
    21361 cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagtta
    21421 tgcctatcgt tacctgtgta acacttccag ccgtaccgat cttaactatc tacaaggcgt
    21481 ggatggcccg attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc
    21541 tatcagtcca ggccacatga accagctgcg ggctgaaagc attccggaag atgtgattgc
    21601 cggagcctcg gcactggttc tcacctctta tctggtgcgt tgcaaaccgg gtgaacccat
    21661 gccggaagca accatgaaag ccattgagta cgcgaagaaa tataacgtac cggtggtgct
    21721 gacgctgggc accaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa
    21781 agatcacgtc tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag
    21841 cgatccgttg ttggcatctg acaaggcgct agactgggta gatctggtgc tgtgcaccgc
    21901 cgggccaatc ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca
    21961 gcatccgctg ctgccgggcg ctatagcgga attcaaccag tatgagttta gccgcgccat
    22021 gcgccacaag gattgccaga atccgctgcg tgtatattcg cacattgcgc cgtacatggg
    22081 cgggccggaa aaaatcatga acactaatgg agcgggggat ggcgcattgg cagcgttgct
    22141 gcatgacatt accgccaaca gctaccatcg tagcaacgta ccaaactcca gcaaacataa
    22201 attcacctgg ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag
    22261 ctatcaggta ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg agcgtgaaga
    22321 cagcctggaa gagtcttact gggatcgtta agttatcgtc ggttcgtagg ccagataagg
    22381 cgttcacgcc gcatctggca tttggctctc gatgcctgat gcgacgctgg cgcgtcttat
    22441 catgcctaca tatttttcat attttacatc cggcaaccac cgtttacccc gtcaccacct
    22501 cacccgccgg tggcgtttcc agcagttcca gcatggtacg ggcgatttca cgctcgccca
    22561 tcactacctg attcgcacca cgttcggtga tatacgccac ttcatcgtca taatgggcgc
    22621 gggcaataat ctcaatatcc ggatttttcg cgcgggcaga tgccacaatc tcacccgctt
    22681 cataaccgtt gggaatcgtc aggatcagcc attttgcaca ttccagatgc gccagttgca
    22741 taatttcttc gttcgccgca ttgcccaata ctgcgcggac cccgcgctct cgcagctcat
    22801 caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg agcaatttct
    22861 cccccagcag gctgcctaca cgaccgtaac ccaccagtag cgcatggttg caaatatcca
    22921 ctgggatctg cttctcttct tcgattgcct cttccagcgt ctgctcttcc agcgtttcgg
    22981 tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata atcgacagga
    23041 tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat gccattccca
    23101 gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg atggttaatg
    23161 ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa tgcggctaac gacttaccaa
    23221 acagaataat cgccagcgtc gccagcactg ccagcggttg ctgaatcaga attaacggat
    23281 caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc aatggcagcg
    23341 tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg gcaaagaacg
    23401 caccgagtgc aaaggagaca tcaaacagct ctaccgcacc aaaggcaatc cctaacgcca
    23461 gcgccagcac cgacagggta aacagctcgc gagaaccggt tgccgcgctg cgtgccataa
    23521 tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg atcactttgc
    23581 cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc tgttccatca
    23641 ttcctgccac tgcgggcagc aacaccagcg tcagaaccat taccaggtct tccacaatca
    23701 accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct tcaagtgcgc
    23761 gcagtaacac cacggtactg gcggtggaaa gacataaacc gaacacgata ccggtcatta
    23821 acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg gctatctggg
    23881 cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc gaaaagtgca
    23941 aaccgacgcc aaacatcaac agaatgacgc ccagttcagc cagttccggc gcaagcttgg
    24001 tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac agatatccca
    24061 ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg agcacaaggc
    24121 cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct tttcctggtg
    24181 gttattgtcc atttttggcc gggaaaacca aaattacagg taatagttta tgacaatttc
    24241 attgatgatg ttcatgaata attattgaat tttgcagaaa aatggaatta gctgcaaaaa
    24301 aagcacggat cggaaaacag aaggcggttt aacagaggaa aggcttatgg cgactgtaaa
    24361 acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc cggttatcag
    24421 gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta tagactaact
    24481 cgatgctggt gtgagcggcg ataagcccca gaactgccgc tcccagacct cccatgccaa
    24541 aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagt agctcctgag
    24601 cgtagaccag aatggcagag aatgccgaag cgaggataaa tccaataatc accgttaaaa
    24661 cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt aaacggcgca acgccgagga
    24721 tagagcccca aatcacatat ttccgtccaa ttttatcccc tacaggcccg ccgatcaccg
    24781 tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgggcattc tggatagata
    24841 atccgaattt ttgcatcaga taaaaggtgt aatag
//
LOCUS       DADLTD010000040        24834 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLTD010000040 DADLTD010000000
VERSION     DADLTD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.040"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            108..455
                     /gene="grxD"
                     /locus_tag="JN701_001305"
     CDS             108..455
                     /gene="grxD"
                     /locus_tag="JN701_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY7363813.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(506..4963)
                     /locus_tag="JN701_001306"
     CDS             complement(506..4963)
                     /locus_tag="JN701_001306"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAY7363814.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLLLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTVRPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQRLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DGIVARIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGR
                     TPGHRTPLWQQRLRASQLLEIAQGYPDFPVILEILRECLQDVYDLPALERLMRRLNGG
                     EIQISDVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVD
                     PGELLDPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEV
                     ASYLENLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLR
                     DLFLRYLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRR
                     LRLRSLQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMR
                     VIEQLAGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVAL
                     HLQEYAAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSA
                     LQEALWALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPISPLVS
                     YNTPNLAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTL
                     CRSMEDSGRIMRGRFVEGLGGAQFAERLTIDRWRDLATQATQTRHYTPVALSANDPAN
                     VWGNLLPWPAHPATLVPTRRAGALVVVSSGKLLLYLAQGGKKMLVWQEKEELLAPEVF
                     HALTTALRREPRLRFTLTEVNDLPVRQTPMFTLLREEGFSSSPQGLDWG"
     gene            complement(5056..5703)
                     /gene="rnt"
                     /locus_tag="JN701_001307"
     CDS             complement(5056..5703)
                     /gene="rnt"
                     /locus_tag="JN701_001307"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY7363815.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5806..6213)
                     /gene="gloA"
                     /locus_tag="JN701_001308"
     CDS             complement(5806..6213)
                     /gene="gloA"
                     /locus_tag="JN701_001308"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY7363816.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6294..7391)
                     /gene="nemA"
                     /locus_tag="JN701_001309"
                     /pseudo
     CDS             complement(6294..7391)
                     /gene="nemA"
                     /locus_tag="JN701_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(7428..8027)
                     /locus_tag="JN701_001310"
     CDS             complement(7428..8027)
                     /locus_tag="JN701_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7363817.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGAHGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8130..8369
                     /locus_tag="JN701_001311"
     CDS             8130..8369
                     /locus_tag="JN701_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY7363818.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8418..9314
                     /locus_tag="JN701_001312"
     CDS             8418..9314
                     /locus_tag="JN701_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY7363819.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9395..9916
                     /gene="sodC"
                     /locus_tag="JN701_001313"
     CDS             9395..9916
                     /gene="sodC"
                     /locus_tag="JN701_001313"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY7363820.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9917..10759)
                     /locus_tag="JN701_001314"
     CDS             complement(9917..10759)
                     /locus_tag="JN701_001314"
                     /inference="COORDINATES: protein motif:HMM:NF016513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY7363821.1"
                     /translation="MAAISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQI
                     SDLWQFLLFLFPLLATMQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLND
                     NLAKIVGVALAWLAFAILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTY
                     HHVSQLSNSQDALARRWLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRG
                     VMSERGVQQKSLAATLEELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQG
                     TQAS"
     gene            complement(10784..11464)
                     /locus_tag="JN701_001315"
     CDS             complement(10784..11464)
                     /locus_tag="JN701_001315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363822.1"
                     /translation="MIVGILCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQ
                     PETNDAIRAAHEGGIGQILTMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISS
                     LRRMLLNWPSPPGATREILEQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQ
                     RLRYFCRLYLQSSQELHRLQSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMI
                     GAWSIASQ"
     gene            complement(11863..12300)
                     /locus_tag="JN701_001316"
                     /pseudo
     CDS             complement(11863..12300)
                     /locus_tag="JN701_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(12366..12539)
                     /locus_tag="JN701_001317"
     CDS             complement(12366..12539)
                     /locus_tag="JN701_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY7363823.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            12740..13174
                     /gene="slyA"
                     /locus_tag="JN701_001318"
     CDS             12740..13174
                     /gene="slyA"
                     /locus_tag="JN701_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY7363824.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13221..13688)
                     /gene="slyB"
                     /locus_tag="JN701_001319"
     CDS             complement(13221..13688)
                     /gene="slyB"
                     /locus_tag="JN701_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY7363825.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            13962..15071
                     /gene="anmK"
                     /locus_tag="JN701_001320"
     CDS             13962..15071
                     /gene="anmK"
                     /locus_tag="JN701_001320"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY7363826.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15169..15498
                     /gene="mliC"
                     /locus_tag="JN701_001321"
     CDS             15169..15498
                     /gene="mliC"
                     /locus_tag="JN701_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY7363827.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15557..16213
                     /gene="pdxH"
                     /locus_tag="JN701_001322"
     CDS             15557..16213
                     /gene="pdxH"
                     /locus_tag="JN701_001322"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY7363828.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16342..17616
                     /gene="tyrS"
                     /locus_tag="JN701_001323"
     CDS             16342..17616
                     /gene="tyrS"
                     /locus_tag="JN701_001323"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY7363829.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17678..18538
                     /gene="pdxY"
                     /locus_tag="JN701_001324"
     CDS             17678..18538
                     /gene="pdxY"
                     /locus_tag="JN701_001324"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY7363830.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(18582..19187)
                     /gene="gstA"
                     /locus_tag="JN701_001325"
     CDS             complement(18582..19187)
                     /gene="gstA"
                     /locus_tag="JN701_001325"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY7363831.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19293..20795)
                     /gene="dtpA"
                     /locus_tag="JN701_001326"
     CDS             complement(19293..20795)
                     /gene="dtpA"
                     /locus_tag="JN701_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY7363832.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21406..22041)
                     /gene="nth"
                     /locus_tag="JN701_001327"
     CDS             complement(21406..22041)
                     /gene="nth"
                     /locus_tag="JN701_001327"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY7363833.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22041..22736)
                     /locus_tag="JN701_001328"
     CDS             complement(22041..22736)
                     /locus_tag="JN701_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY7363834.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(22740..23360)
                     /gene="rsxG"
                     /locus_tag="JN701_001329"
     CDS             complement(22740..23360)
                     /gene="rsxG"
                     /locus_tag="JN701_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY7363835.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23364..24422)
                     /gene="rsxD"
                     /locus_tag="JN701_001330"
     CDS             complement(23364..24422)
                     /gene="rsxD"
                     /locus_tag="JN701_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY7363836.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24423..>24834)
                     /locus_tag="JN701_001331"
     CDS             complement(24423..>24834)
                     /locus_tag="JN701_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7363837.1"
                     /translation="QQQANAEPEEQVDPRKAAIEAAIARAKARKLEQQQANAEPEEQV
                     DPRKAAVEAAIARAKARKLEQQQANAVPEEQVDPRKAAVEAAIARAKARKLEQQQTNA
                     EPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         6156 a         6419 c         6461 g         5798 t
ORIGIN      
        1 aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg
       61 ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta
      121 tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac
      181 cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg
      241 aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat
      301 atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg
      361 atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg
      421 ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacggatgc
      481 gaacgggcat ccgtcagtcc tttctctatc cccaatccag cccttgtggc gaacttgaaa
      541 atccctcctc acgcagcagc gtaaacatcg gcgtttgccg gaccggtaga tcattcactt
      601 ctgttagcgt aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa
      661 cctctggggc gagtaattcc tctttttcct gccagaccag catttttttg ccaccttgcg
      721 ccagatagag taacaatttg ccgctagaaa cgaccaccag cgcacccgcc cgacgcgttg
      781 gaaccagcgt tgccggatgt gcaggccagg gcagaagatt tccccacaca ttagccggat
      841 cgttggcaga gagcgccact ggtgtgtagt ggcgcgtttg cgtggcttgt gtcgccagat
      901 cgcgccatcg gtcaatagtc agacgttcag cgaattgcgc gccacccaga ccttctacaa
      961 aacgacctcg cataattcgc ccggagtctt ccatacttcg acaaagcgtt tgcatcgatg
     1021 gaaacccgcc agggatattt tcggctatca ccgcctgacg actgatgatg ccgtagcggt
     1081 cgagcatatt ttccgccagc gccagcatcc tttcggtatc gtttagtggc tccacctgca
     1141 ataacgacca gcgtccagcc agatttggtg tgttgtaaga taccagcggc gagatgggtt
     1201 gcgcatagac aggacgtcca cgacgagccc ggtgactgcg gcgagttgag gtgcgtgcgt
     1261 tggaactgct gcgggtgagg gcgcgtaacg gtgcccaaat gtcgctggtg atgacgcctt
     1321 gccagaccag cgcccataac gcctcttgca gggcagagag atcaaccgat tcgccgattt
     1381 tgtcgcgtat ccgttggctg atttgttgtg caaaccaggc tcctccgtca gccagaacag
     1441 cgactatcgc ttgttgcagc gccgaacgat tcgcctgatc cgcttcggcg ggagtgaacg
     1501 attctgcagc atattcctgt agatgcagtg ccaccaggcc gtcatcttca cccagttttt
     1561 tttgccccga ccagataacc gcaccggttg ccagtaattc atcgagcatt tctgacgaat
     1621 agtcgcgtac gcgggcaggc aggatctggc tttcccagag tgaggcgggt aaaccgactc
     1681 cggcaagctg ttcgatcacc cgcatcacgc catctacgcc ctcataaacg cctggcgatg
     1741 ttgaggcaaa gagcgccggg ctaccatcgg tggcgggtaa tacgccctga cgttccagca
     1801 gcaatcgcgc ataggtcgtg gctgcaacgg gacgcgtcgc ttctctggcg gcttgcagcg
     1861 agcgcaaacg cagacgacga aatacttcat cgctcaccca gatgtcttgt tgcagattca
     1921 tcaccagacc ctgttcacgc agttgctgaa gctgctcttc gacaatggca ataccgagac
     1981 taaactcatg agccagttgt tcagccgtga ccagagcatg agcccggaga tagcgcagaa
     2041 agaggtcgcg aagcgggtaa ctgactctat gtaaataaat ctctggcaat gactctggta
     2101 gtcgtacgcc gagggcatca cgcagcctgg cggcatcatc catacaggca agacgctcct
     2161 gtccgctaat catcgctggg aagattcgtt ttactgcaag aagattttcc agatacgacg
     2221 caacctcttc actgctgcct gtatgccgtt gcgccaggtc ttcaacggtc attggcccca
     2281 gttcgcgcag caggtcgaac aatccttctt cacctttcgc tcttctgcca ggagccagtc
     2341 gttgcaactc ttcttccacc tggcgaatga cctgcgggtc gagtaattcc cccggatcga
     2401 cctgtccgag tagattgcgc agtaactcgc tgtccagcga cagtacggat gcccggcgct
     2461 ctgccagcgg ggcgtcgctc tggtacataa attccgcgac atagccgaac aataaacttg
     2521 tggcgaaagg cgagggcgta gtggtcgtta catcggatat ttgaatttcg ccaccgttca
     2581 ggcgacgcat caaacgttcc agtgcgggaa gatcataaac atcttgcaga cattcgcgta
     2641 ggatttcgag aatgaccgga aaatctggat atccctgagc gatttccagc aactgactgg
     2701 cgcgcagccg ttgttgccaa agcggggtgc gatggcccgg agtgcgcccc ggcattaata
     2761 atgcccgcgc ggcgcattcg cgaaaacggg cggcgaaaag tgccgagctg cctaccgcct
     2821 cgcggacaat ttgcagcaac ttttctggtt caaacaaaaa aatcgcggcg tcgggcaatt
     2881 taccatcagt gtcaggaatg cgcgcaacaa tgccgtcatc actggcgacc acagacgcgt
     2941 cagcgcccca tagggcatgt attcgcccgg caatcgccag cgcccagggt tcatgcaccc
     3001 gtcttccata gggagagtgc aaaataatac gccagtcgcc aatttcatca cggcaccgtt
     3061 cgaggaccag atggcgactc cccggaacga tgcccgtcgc gttgcgttgc tcgtcaatca
     3121 acctctgaat attggcgttc gtattttctt ctgccagcca cgggggaata gtcccggaaa
     3181 agaacgcgcc atccgccagt aaatgaagaa aatcgccgat catctcgcct aattcagccg
     3241 gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc ggagcaggag
     3301 tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg ccgagagtga
     3361 taatgtcgtt cacccgcgac tcatatacca tctcctcatc cagttcaccc acccgccgcg
     3421 aaccggcctt ttcttcacct tcgggtaata acacgctata catgccacga tccggaatgg
     3481 tgccgccgct ggtaaccgcc aacaattgag cgccaggtcg gacggtcaat atcccggtct
     3541 ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga tagcgcccgg
     3601 aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac ggtgcggcac
     3661 ggcgtacgcg ggagtaccat tcgtctacct gtaatgcctc catcgccgcg gcggcaacgg
     3721 tttgctgcgc aaggacgtcg agtggattat gcggcggtgt caggttttcc agcctgcctg
     3781 cgaacataca ctctacaatg actgcggaat cgactaagtc acgccgggta cgagggaaaa
     3841 acagcccttt agatacaccg ccaacctgat gtccggcgcg accaatgcgt tgtaacccac
     3901 tggcaacaga aagcggcgtt gccacctgaa tcaccagatc cactgcgccc atatcaatcc
     3961 ccagttcgag actggaggtt gcgaccacgc agcgtaactc acccgatttc agcgcctgct
     4021 cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca ataaaaacgt
     4081 cgctactttg tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg gccgcatcaa
     4141 cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat cgtgccgtca
     4201 gtttttccgc cagcccacgt gaattagtaa agacaatggt cgagcgatgg cgcaacactt
     4261 catcaaggat acccgtttca atatatggcc agatggagcc ttcccggccg gcatggctgt
     4321 cttcgccggt gctgctggcg accgatgaga catcatccat attggcgacc ggtacgacaa
     4381 ttcgtatctg cggatggcgc attgcgggcg ggttgactac cgtaaccggg cgatcgccac
     4441 caagaaaggc tgccacatcg ctggctgagc gcacagtggc agaaaggcca attcgctgtg
     4501 ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc gcaccacgtt
     4561 tactgcccgc taccgcgtgg acttcatcaa taattaccgt ttcgccgcgc cgccgccgtt
     4621 catcggcaat acccttcaac aggagctgca agttgcgctg aacgtcggtg cccagagctt
     4681 ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt
     4741 cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg
     4801 agccggtcgg tgcaatcacc agcgcatgtt cgcttcgcgc cgccacatgc caggtttgcg
     4861 actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa
     4921 acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct attccccgtg
     4981 aatatcatgc aggatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat
     5041 caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt
     5101 ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga
     5161 gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt
     5221 ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg
     5281 gttacgtttc agtgaggcgc gttctgcggc ggctatcata aagctgtgat caaaattggc
     5341 attgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt tacgtacaac
     5401 tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ccacgatcgg gatcgttcgg
     5461 gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa atggttcgac
     5521 gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat
     5581 ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt caacatcgat
     5641 cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga
     5701 catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg
     5761 atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt tgcccagacc
     5821 gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat cttccataaa
     5881 cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt
     5941 ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga tgtgaccata
     6001 agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc
     6061 ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct
     6121 ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcaaatc
     6181 gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga
     6241 ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt ggattacaac
     6301 gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc acgctgtggg
     6361 ttaagctcag ctttgcgctg caagcgggcg accagatccg ggttcgcaat ccagtcacga
     6421 ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt ttctactgtg
     6481 tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca
     6541 tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg
     6601 cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg gccgttatcc
     6661 gtgttctgga aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc accccattct
     6721 tcaatcccgg catcgaccac ttccagtacc agacgtgcgc gattttccac gctgccgccg
     6781 tactgatcgg tacgatggtt tgaagaagga gaaaggaact gatgcagcaa ataaccgtga
     6841 gcagagagga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg
     6901 aaatcattga cgatacctgg aatctcttct agttcaagcg cacgcggcat ggatgtttca
     6961 acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct
     7021 gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg
     7081 tgccacagct acacggccat atgaccattt tcagcatgaa cgccagcggt gatttttttc
     7141 catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc ttttacctgg
     7201 gcagaaattt gcgtggcttc actaataatc aaaccggcac tggcacgttg gcgatagtat
     7261 tccgccatca acggggtagg aatgtcaccc ggttcaatac tgcgcagacg cgtcagcggt
     7321 gccataaaaa tacggtttgc cgccgtgatc gcgcccactt tcagtgggga atacagtttt
     7381 tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta aacggcaggc
     7441 gtcgcaataa tgttttttac atgggccagc gcgttttcca gtggctcgaa actgcgcgaa
     7501 attttggcct gcagattcgc acccagccat agtgcgtaaa gcacttgtgc ctgttgtagc
     7561 ggttcgccac aaaaggttaa acaatggttc tcacggccat tttccagcgc ctgagagagc
     7621 agggcgatca cgccgtgagc acctttatcc atcgcgctgc gcatatcttc tgacagatcg
     7681 cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg ttggcaaaac
     7741 tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc ttcgccggat
     7801 tgcagcaact cagtcagtcg ctggtgatat gcggtgtaat gacgctcaag catggcaacg
     7861 ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac ttcagcggtt
     7921 tttagtaatt cgcttagccc catcccggtg aatccacgtt gcaggcaaag ttgctcgccc
     7981 gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta
     8041 gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca gcttcaataa
     8101 tggtaataat cttatctgag gagggcgaag tggcggagca attagagttc tttcccgtcc
     8161 agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc
     8221 gtagtcggga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa caagaggtgc
     8281 tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc aaataagcca gcgtcatcag
     8341 acgaaccgga gcaaccatca ctcttttgaa cctaatcttg cgtatactcc tctcatatct
     8401 gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc ccggagtttt
     8461 cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc cgccagctgg
     8521 tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat gctgatattt
     8581 atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca cctcacctgc
     8641 gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg
     8701 tcattggtca ttacatcact gaccgcgatc acatcattaa gagcgccgaa cagtcgctaa
     8761 ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac ccgttaatgg
     8821 atgccgatga agtggcggac gcgttcaaac atctgcatca gagcggcaaa gtgcgtcatt
     8881 ttggcgtatc gaactttacg cctgcgcaat ttgccctgtt gcaatcacgt ctgccgttta
     8941 cccttgccac taatcaggtg gaaatatccc cggtgcatca gccgttactg ctggatggca
     9001 cgctcgacca actacaacaa ctgcgtgttc gtccgatggc gtggtcctgc cttggtggtg
     9061 gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct gtggtggcag
     9121 aggagttaaa cgcgggctcg attgaacagg tggtttacgc ctgggtatta cgtttaccat
     9181 cgcagccgct gccaattatc ggttcaggta aaattgagcg cgtacgggca gctgtcgaag
     9241 cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat tcgtaaagcg gcactggggt
     9301 acgacgtacc gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc actggtttac
     9361 acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc tggctattct
     9421 ggcgctggtt gttgcaaccg gcgcacaagc tgccagtgaa aaagtcgaga tgaacctcgt
     9481 cacgtcgcaa ggggtagggc agtcaattgg tagcgtcacc attactgaaa ccgataaagg
     9541 tctggagttt tcgcccgatc tgaaagcatt accccccggt gaacatggct tccatattca
     9601 tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg cggaatccgc
     9661 aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag gtgccgggca
     9721 tttaggcgat ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg atgccgtcat
     9781 cacgcctcgt ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg tccacgttgg
     9841 cggcgataat atgtccgatc aacctaaacc gctgggcggt ggcggtgaac gctatgcctg
     9901 tggtgtaatt aagtaattaa gaggcctgcg taccttgcgg tggtgcttgc tcaagttgcg
     9961 aaagcgagca gtacagccgc cagacaattg ccgccagctc acgggcggca ggttgatgat
    10021 gacgggcaag gctgtcgcaa atccgctgta attcttcaag tgtggccgcc agtgattttt
    10081 gctgaacgcc acgctcactc atcactcccc gcaacagtga aatacagtta tcccgtactc
    10141 gcgataacgg atcggaacgc gattcccagt cgcgcaattg ccagacaaca tgagaacagt
    10201 tcagcagcac tacaccccag cgtaataacc aacggcgagc cagcgcatcc tggctgttac
    10261 tcaactgact gacgtgatga taagtgagcg attcaaattc gctttcactc agtgttggat
    10321 ggcggcttag ctgatcgaca aaatcccggc gcagcgcgcg aatatggcgg cggcttttac
    10381 gagcatccga tcctggacgc agaatggcga acgctagcca agccagcgca acgccaacga
    10441 tttttgccag attatcgtta agaaagtcag caaaatcata caccggggga ttagtgacag
    10501 cgataaacga acccataaaa acaatcagtt gcccccacag tgcggcaaat ttgggcatct
    10561 gcaatttaag aagctgcatt gtcgccagca gtggaaagag aaacagtaaa aattgccaca
    10621 gatcgctaat ctggaccatc aggccaaatt tgaccacaaa gctgaatagc gaaagtaaca
    10681 ccagcgtgcg catcagcagt gacaacgact taaacggtgc tgcgacggcg gagtagagta
    10741 cgcagctaat tgctgccagc gttaatgcat tggcaccggc atcctattgc gatgcaatac
    10801 tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag cccgctccac atagcttcgg
    10861 cgttgtcggt atgacgagcc agagcggatg tccgtgcgag tctggcatga tcatctacac
    10921 cgctttgcag acgatgtaat tcctgactac tttgcagata aaggcggcaa aaataacgta
    10981 gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt agcggggcga
    11041 taatacgtgc gacggtgtaa gcatctgttt gcgaactggc gagcgccgtc agcaattgtt
    11101 cgagaatttc tcgcgtggca cctggcggtg agggccagtt gagcaacata cgtcgcaggc
    11161 tggagatgac actggtcata cgtaattgct ggtggagtag cgcattaagg cgcgcgtttt
    11221 gctggcgaaa acgatagtgg ctccagaaag cctggatacg cagcaaattc atggtcagta
    11281 tctgcccaat ccccccctca tgtgctgcac gaatggcatc gtttgtttca ggctgccaga
    11341 gtaaactggc atgttccagt aatcgggcgt gcatgttttt caatgcggtt aaaagggctg
    11401 tagcatcgga actgctaggc aggatcatca tcatcatgcc gccgcacaaa ataccgacta
    11461 tcacctcgca aacgcgcgcc tgagcgatat cctacagctg gctggcctca gtaatattaa
    11521 ccatcggaaa ggcgatgatg gcagccgtgt agccgccagt tgaaatgcat acgcgacgtt
    11581 attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca tgctcaatag
    11641 aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa tggctcccag
    11701 taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg tgggaaagct
    11761 aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat aataggcaac
    11821 cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt gcgctagcgt
    11881 ggcctgtgcg gcattaacac tggcctgcat cgctttaaca ttgaggttag cggtatcgag
    11941 ttcttcggca gaaataaaat tttgtgagag atgacgacga cgattcgctt cgttattggc
    12001 ttttgcgagg tcagattgcg ctttcgccag ctgagcctgt gcgttcagct cggcgatctg
    12061 aaagggcgtt ttatcgatcg tgagcagaag atcccccgcg ttaaccagtt ggttgtcttt
    12121 tatattcagc tcaacaatac gtcccgacac ctgtggggtg atagaaacct tttctgcgcg
    12181 tattttcccg tcgcgtgtcc acggagattg catgtaataa ttccacagcc accatccggc
    12241 aagaaccgca actatcgcta caatgattat tgagaaatac ttaattgttt taattgacat
    12301 agttaccacg caatcagtat tgccagagca aggcaaacgc aaatcgcaaa cagcgataaa
    12361 tccatttaca agggatgcca gatgtcaccg gcgtagatcc agccacgaag caggcggtgt
    12421 acgacaagcc agatgacgaa tccaaacgcg aaggctttga aaaacggagg aaaatagacg
    12481 gacgcaccga acaccagatc ttgtaagggc aatcctgttg cattgagcat aaacttcaca
    12541 aagtagattc ctttacgacc gaatatattg cgttagatta ataaatattc tttaagtgcg
    12601 aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt aaccaaatgc agcaatacat
    12661 ttgttttagc aataaaattg ctgcacacta ttctaaaagc cgcataatat cttagcaagc
    12721 taattataag gagatgaaat tggaatcgcc actaggttct gatctggcac ggttggtgcg
    12781 catatggcgt gctctgatag accatcgcct gaaaccgctg gagttaacac aaacccattg
    12841 ggttacgtta cacaatatcc atcagttacc tccagaccag tcgcaaattc aactggcaaa
    12901 agcgattggc atcgagcagc catcactggt ccgtactctg gaccaactgg aagaaaaagg
    12961 gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct aaacgtatta aactgacgga
    13021 aaaggcagag ccactgatca gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat
    13081 attacatggc atctccgcag aggaactgga gcaactgatt acgctcatcg caaaacttga
    13141 gcataatatc attgagttac aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac
    13201 ctgaccacac gcaaacttat ttagcgcgga gaaacggtca cctgactgcc attgctggcc
    13261 agtacgacac gttggcccgg agagaaacga gtgttgcctt gtttctgtac caccatgatg
    13321 gtattaccat cgtctttacg aatttccagc tcgacaccct gcgttttgtt cattgcactc
    13381 tgtacgccct gaccagctac gccacctgca acagcgcctg ctgcagtagc cagagaacgc
    13441 ccggttccgc caccaacagt attccccagg aaaccaccaa gaacagcacc gccaattgca
    13501 ccgataacgt tggaatcatc accgccctga atctgtaccg gacgtacgtt aacgatggtg
    13561 ccatagctga cattctgtac ttgtttcgct tcagaagcgg tataaacatc ccctgacagg
    13621 gtgtcgttat taacacaacc gacaagagac agacctacca ttgaaacaac caatacgcgt
    13681 ttaatcattg aaaaatctcc tgttcaccat gaaacgccac gcaagcatcc ctcatggtca
    13741 aagtatatgg catatgtgaa tccatggtta catgttctac caaatatgag caaatcataa
    13801 tgaaagtagg atcaaccaat tataaacaaa acattcatga tgattattga tttgaagata
    13861 atgttttact accaacggta aaaatcggtc attccgcatg gtattcacga aaagtttatg
    13921 tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac tatgaaatcg ggccgcttta
    13981 ttggcgttat gtcaggcacc agccttgatg gtgttgatgt tgtgttggcg acaatagatg
    14041 aacaccgggt cgcacagctg gcaagcttga gctggccgat cccggtgtct ctgaaacagg
    14101 ctgtactgga tatttgccag ggccagcagc ttacactttc gcagtttgga cagcttgata
    14161 ctcaactcgg gcgacttttt gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc
    14221 aggcgagaga tatagttgcg atcggctgtc acggtcaaac cgtctggcat gaaccgacgg
    14281 gcgtggcacc acacacttta cagattggcg ataacaatca aattgtggca cgcaccggaa
    14341 ttacggttgt cggtgatttt cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc
    14401 tggtacctgc gttccatcat gccttgctgg ctcacccaac cgagcgacga atggtgctca
    14461 atattggcgg catcgccaat ctgtcactgc tcattcctgg gcagccggtt ggcggctacg
    14521 ataccggtcc tggtaacatg ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt
    14581 acgataaaga tgccgagtgg gcacgggcgg gtaaagttat tctcccactg ctgcaaaata
    14641 tgctcagcga cccatatttc tcgcaacctg caccgaaaag caccggacgc gaatacttta
    14701 actacggctg gctggagcgc catttacgcc attttccggg tgttgatccc cgtgatgttc
    14761 aggccacact ggcagaactc accgccgtga ccatttctga acaagttttg ttgagcggtg
    14821 gctgcgaacg attgatggta tgtggtggag gtagtcgtaa tccgctactc atggcgcgtc
    14881 tggcggcatt actgccaggc acagaagtca ccaccaccga tgccgttggc attagtggcg
    14941 atgacatgga agcattggct ttcgcctggc ttgcctggcg gacgctggcg ggattaccag
    15001 gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt actgggggct attttccctg
    15061 ctaacccgtg atatcagagt taactgaatt tttcttaccg cgtctgccgt tacacttgga
    15121 acgttaggga gggcgtattg ccctccagac caggaaagtc ttcgggaaat gtctatgaaa
    15181 aaactgttac tgatctgttt gccggtgctt ctcaccggct gtagcgcgtt caatcaactg
    15241 gttgagcaca tgcaaaccgc tacgctggaa taccagtgtg atgaaaaacc gttgacggtc
    15301 aaactgaata atccgcgcca ggaagtcagt tttgtttacg ataatcaact actgcatctc
    15361 aaacagggca tttcagcgtc tggtgcacgt tacactgacg gaatctatgt tttctggtcg
    15421 aaaggcgatg aagcgactgt ctataaacgc gaccgcatcg tattgaataa ctgtcagtta
    15481 caaaatccac agcgttgaga tttttccagg ggcggcgcac aatagcgtca cccactgaca
    15541 atccgtaaag aaaaccatgt ctgataacga cgaattgcag caaatcgcgc atctgcgccg
    15601 tgaatacacc aaaggcgggt tacgccgtcg cgatcttccc gccgatccat taaccctttt
    15661 tgaacgttgg ctctctcagg cgtgtgaagc caaactggca gaccctaccg cgatggtggt
    15721 cgctaccgtg gatgaacatg ctcagcctta tcaacgcatc gttttactca aacattacga
    15781 cgaaaaaggc atggtgtttt acaccaacct cggtagccgt aaagcacatc aaattgaaaa
    15841 taatccgcgc gttagcctgc tgttcccgtg gcataccctt gaacgccagg tgatggtgat
    15901 cggtaaagca gaacgacttt cgactctcga agtgatgaaa tattttcata gccgcccgcg
    15961 tgatagccag attggtgcat gggtttcgaa gcagtccagt cgcatttctg cccgcggtat
    16021 ccttgaaagt aaattcctgg agctgaagca gaagtttcaa cagggcgaag tgccattgcc
    16081 gagcttttgg ggcggttttc gcgtcagcct tgaacagatt gagttctggc agggcggtga
    16141 gcatcgcctg catgaccgct ttttgtacca gcgagaaaat gatgcgtgga agattgatcg
    16201 tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg ctggtactcc tgattctggc
    16261 actttattct atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc
    16321 gttatataca tggagatttt gatggcaagc agtaacttga ttaaacaatt gcaagagcgg
    16381 gggctggtag cccaggtgac ggacgaggaa gcgttagcag agcgactggc gcaaggcccg
    16441 atcgcgctct attgcggctt cgatcctacc gctgacagct tgcatttggg gcatcttgtt
    16501 ccattgttat gcctgaaacg cttccaacag gcgggccaca agccggttgc gctggtaggc
    16561 ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc
    16621 gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc aggttgcccc gttcctcgat
    16681 ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact atgactggtt cggcaatatg
    16741 aatgtgctga ccttcctgcg cgatattggc aaacacttct ccgttaacca gatgatcaac
    16801 aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt cactgagttt
    16861 tcctacaacc tgttgcaggg ttatgacttc gcctgtctga acaaacagta cggtgtggtg
    16921 ctgcaaattg gtggttctga ccagtggggt aacatcactt ctggtatcga cctgacccgt
    16981 cgtctgcatc agaatcaggt gtttggcctg accgttccgc tgatcactaa agcagatggc
    17041 accaaatttg gtaaaactga aggcggcgca gtctggttgg atccgaagaa aaccagcccg
    17101 tacaaattct accagttctg gatcaacact gcggatgccg acgtttaccg cttcctgaag
    17161 ttcttcacct ttatgagcat tgaagagatc aacgccctgg aagaagaaga taaaaacagc
    17221 ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg tgactcgtct ggttcacggt
    17281 gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt
    17341 gcgctgagtg aagcggactt cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg
    17401 gaaaagggcg cagacctgat gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt
    17461 caggcgcgta aaactatcgc ctccaatgcc atcaccatta acggtgaaaa acagtccgat
    17521 cctgaatact tctttaaaga agaagatcgt ctgtttggtc gttttacctt actgcgtcgc
    17581 ggcaaaaaga attactgtct gatttgctgg aaataatgca ttaagtggaa agggggagtg
    17641 agaaatcact ccccctggtt tttatacagg gaacatgatg aaaaatattc tcgctatcca
    17701 gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca gagtttccta tgcgccgcct
    17761 gggcgcgaac gtctggccgc tgaacaccgt tcaattttct aatcacaccc agtacggcaa
    17821 atggactggc tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag gcattgccgc
    17881 cattgataaa ttacacacct gtgatgccgt gttaagtggc tatctgggat cggcggagca
    17941 gggtgaacat atcctcggta tcgtccgtca ggtgaaagcc gcgaatccgc aggcgaaata
    18001 tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc
    18061 agagtttcat gtgcggcacg gtttgcctgc cagcgatatc attgcgccga atctggttga
    18121 gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg
    18181 cgaactcatt gcgcaagggc cacaaattgt gttggttaaa cacctggcgc gagctggcta
    18241 cagccgtgac cgttttgaaa tgctgctggt caccgccgat gaagcctggc atatcagccg
    18301 tccgctggtg gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt
    18361 actgctggtg aaactgcttc agggggcaac gctgcaggag gcgctggaac atgtgaccgc
    18421 tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt
    18481 ggctgctcag gatcgtattg ccaaaccaga acattacttc agcgcaacaa agctctgaaa
    18541 tatctcattt cggcccacaa cgacctgtgg gccgatggct attactttaa gccttccgct
    18601 gacagtgcgt cttgtacttc cggacgttca gccatacgtt gcataaatgc tgcaatgtgc
    18661 tctaaccctt ccagattcag tttcaccgcg tatgcccagc gcagaaccgt aaacagatag
    18721 gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct catccttcag tgtctcgttc
    18781 acatattgca gcttcttctc cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta
    18841 tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg tggcgatgta attcagccat
    18901 tcgatggttt tatagcggga aatactgttt accggtgcca gcaactggcg gtcggggacg
    18961 ctgtcggcaa gatactgcat aatcgctacg ccttccgtca gcaaagtacc gtcatccagc
    19021 agcaatgcag gcacctgccc cttagggtta acggcaaagt aatcgtcacc gttttcgaga
    19081 cgttttttca ttaaatccac actgacgagg gtaaaatcct ttccgctctc acgcagggtg
    19141 atatgggaag cgagagagca ggcacccggt ttgtagaaca atttcatcgg taactccttt
    19201 tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa
    19261 gttagcggct tgtaaaagag tttccctaaa ttttacgcta cagcagcttt cgccgctttg
    19321 tctgcagcgt catcctgcgt catacggtgc agtttaggcg cggtcagcag catcagtact
    19381 gcaataatgg cagtagcgac accaatctgc aagaatacgc gaccataaac ttccagtgac
    19441 atcagcgggt cggtaacgtt atccggcaca gccatcatac ccgcaacata accaccaatc
    19501 aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat cagacgctgc
    19561 ggaacgagtt gtgcaaccat tgccagaccc agaccagaga tcatcagttc cccgatgctc
    19621 tgcaggccat agcttgcgac cagccagctt acagacacga taccagcgtc agacgcgaat
    19681 ttcgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatgcc gattgcaaac
    19741 ttggttggca tcggcagggt atcgcccatc ttgttataga tagcggccag aatcggacta
    19801 ccgatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac ggccagaccc
    19861 agaatggagt gctcaacgtt acgaatcgca aagaagttca gtgacgttgg catctggctg
    19921 tacagcacga agaagataat ggcttcgagc atcaggatga aggcaacgat cattttacga
    19981 cgcgcagcac ctttcatcgc gaaggcttct ttaccgaaga taaccacgat accgaaggca
    20041 acaacgccca gcgccatacg cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg
    20101 atcagtgcca caacaccaat aatggtcagt agcaggttac ggtagttgat aggctcgaag
    20161 tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt aacgatagtg
    20221 atcagcaggc ctacaacgct caacgcaaac gcaacactcc agccgtattt cgcggccagc
    20281 cacggcgtag caatcataga gaagaaagag ccgatgttga cggacatgta gtacatggtg
    20341 aatgcaccgt ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag agaagacggg
    20401 ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata aacgataccg
    20461 gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat agcgccgagc
    20521 ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat agcgaccaga
    20581 ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc agacataccc
    20641 agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc aaaacgttcc
    20701 cataactcaa tcgagaagat gagatagaac gccttcggtt gtttgaaagc gttcaaactg
    20761 acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt tacatcccat
    20821 attaacgggg gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg
    20881 gaggggaaac ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa
    20941 tctgttacat ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta
    21001 aatttttcaa aaaacgtaac ggcagatttt ttcactgcac tgagaggggg gtgatggctt
    21061 aacggcgata tgttctgcta tttagcgttt ttactagtga gatagtccag tttctgaaaa
    21121 atagccagtg taatgttttg taggtcaata tttgttgcgt gtgtaaattt aaatgatttt
    21181 aacctgattc ttgcaatgtt tttacattgc gacagcatcg tgatcttgat cacgaatgaa
    21241 tagaaaattt cgctattaaa aaaacaatta acctgcattt ttggttatcc gcaaacggat
    21301 tacctgatga taacgctaag caatggcatc attcacgtta aaaagatgtt ttaaaagagg
    21361 ataaagaaag gttatcaatg gggtaatcgg tgttacccct tttcttcaga tgtcaacttt
    21421 ctctttgtat tcacaaagat cttcaataat acaagagcca cagcggggct tgcgggcaat
    21481 gcaggtataa cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg
    21541 aaccactttc agtagctttt cttctacctg ttcaacgttt ttccccggcg caaattgagt
    21601 acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc cgaatgcagt
    21661 gtttaatacg acgttggctg ttttacgacc tacgccgggc agggcttcaa gcgcagcacg
    21721 atcttccgga acctcgccat tatgctgctc cagtaagata cggcaggttt tgatgatatt
    21781 ttctgctttg ctgttataaa gcccaatcgt tttgatatag gttttaaccc cttcaacgcc
    21841 cagttcaagc atcgctgcag gcgtattcgc caccgggtag agtttcgccg tcgccttatt
    21901 aacactgaca tcggtcgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga
    21961 actgaaatta agctcggtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc
    22021 caggcgtttt gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg
    22081 gcagctgctt cagtacggcg ctttttcatt ctttcatcaa tcaggtattt tcctgccagc
    22141 atcagtccca ggccaataaa tgcacctggt ggcagcatcg ccagcaggaa aggggagtcg
    22201 gtgtggaaaa tctccacgcg taatactttt gcccagctac ctaacagcgc atctgcaccg
    22261 tcaaacaatg tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag
    22321 gttgcgccca taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg
    22381 gcgaaggctt cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc
    22441 cctaatgatt gatacaggcc aaaggcgtag gcgttgatca gcatctgtac agcgctgacc
    22501 accgaggcga tgatcatcac gtaaatggga atgcggatct cggctggcgt ccagtgacgc
    22561 agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc
    22621 agagcattag tggcagtgga cgtgaccgcc aacagaggac aaaggccgag caactggacc
    22681 agcgcagagt tgtttttcca caacccctga acaataacgt ctttaatttc gctcacgctt
    22741 tattctccac aggcaggaag ttgagaaagt tgtgccggta acgtctgagc gtacaatccg
    22801 gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg
    22861 aaatcaccac catctttctt caccgcccag tgcgcatcat ccgcaccact gattttttta
    22921 cccgcaaaat gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc aagccctggc
    22981 gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc cgttaaaatc ggctcccacc
    23041 agcagctgaa tcgcaccgga atagccatct ggcgcggttg cttccagaac ggcggctacc
    23101 ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca
    23161 gttaccagat agcaactctg tgccagcgca ttgttatagc gttcggctgg cagcacctga
    23221 tcaaataatg ccttttgttg cagacttgcc tgttcagcaa tcgtcgtttt ggtcatctgg
    23281 ttgatggccg cagttaaccc tgttgaaccc gctgcaaata gcgctaacgt aatgccgtgt
    23341 tttcggatag ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg
    23401 tagtaatcga tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca
    23461 ggatagccgc cgaaactgcg gatcaaccag actaataagc ccgcaagcgc gccgaaaata
    23521 agacgaccac gattggtcgt agaagcggta accgggtcag tcaaaataaa gaatgcgccg
    23581 agcatggtcg caccagacag cagatgaatt tgcggtgctg ccagtgtttc tggtgagaac
    23641 aaccagccca acgttgcgca taacgccagc gttactaaga agctgagggg aatatgccag
    23701 cgaatcgctt tctgccatag caaccacagg ccgccagcca gccaggcgag atttacccat
    23761 tgccaaccag cgcccgccag aataccgctg tagatcggat attgcataat ctgttcaacc
    23821 gaatgaccgg cacggacaga ggttttaaat gtatccagcg gtgtcgcctg actaatgcca
    23881 tcaataccta agtgtagtgt gttcatatca gcaccactgg cggtatgccc gctgaaaata
    23941 acctgtatgg cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag
    24001 ctggtcatct gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta
    24061 aacgggtttt gccccagacc gccatacaac tgtttagcga tgatcaccgc aaacaccgta
    24121 cccagcacga ccatccacca tggcgcgagg gggggaatac ttaccgccag caataagcct
    24181 gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg actgcttgcg tagtttgagt
    24241 acgagagctt cggctaacag agtactaacc gatgccaaca ggatctgaac gagagtaccc
    24301 caaccaaaaa accacagttg cgctgcgatt cctggcacgg ctgcgagcaa caccaacagc
    24361 ataatgcgcg atgtctggcg ctggttatgg gtataagggg agctagctat tctgaatacc
    24421 atttagtcct cgtttacaac cttttgctgg gcggcttttt tggcctgagc gcgggcaata
    24481 gccgcggcaa ctgccgcttt gcgcggatca acctgttctt ctggttctgc gttagtctgt
    24541 tgctgttcca gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc ggctttgcgc
    24601 ggatcaacct gttcttcagg taccgcatta gcctgttgct gttccagctt gcgtgctttg
    24661 gcgcgtgcaa tagccgcttc gacggcagct ttgcgcggat caacctgttc ttctggctcc
    24721 gcattagcct gttgctgttc cagtttgcgc gctttggctc gggcaatagc ggcttcgatg
    24781 gcggctttgc gcggatcaac ctgttcttct ggttccgcat tagcctgttg ctgt
//
LOCUS       DADLTD010000041        24163 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLTD010000041 DADLTD010000000
VERSION     DADLTD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.041"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(154..1035)
                     /gene="yddG"
                     /locus_tag="JN701_001332"
     CDS             complement(154..1035)
                     /gene="yddG"
                     /locus_tag="JN701_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY7363838.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1267..4314
                     /gene="fdnG"
                     /locus_tag="JN701_001333"
     CDS             1267..4314
                     /gene="fdnG"
                     /locus_tag="JN701_001333"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1852..1854,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7363839.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4327..5211
                     /gene="fdxH"
                     /locus_tag="JN701_001334"
     CDS             4327..5211
                     /gene="fdxH"
                     /locus_tag="JN701_001334"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7363840.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5204..5857
                     /gene="fdnI"
                     /locus_tag="JN701_001335"
     CDS             5204..5857
                     /gene="fdnI"
                     /locus_tag="JN701_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY7363841.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5908..6192)
                     /locus_tag="JN701_001336"
     CDS             complement(5908..6192)
                     /locus_tag="JN701_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7363842.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6338..7348)
                     /gene="adhP"
                     /locus_tag="JN701_001337"
                     /pseudo
     CDS             complement(6338..7348)
                     /gene="adhP"
                     /locus_tag="JN701_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7482..9179)
                     /locus_tag="JN701_001338"
     CDS             complement(7482..9179)
                     /locus_tag="JN701_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY7363843.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9336..9473)
                     /gene="sra"
                     /locus_tag="JN701_001339"
     CDS             complement(9336..9473)
                     /gene="sra"
                     /locus_tag="JN701_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAY7363844.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(9575..9790)
                     /gene="bdm"
                     /locus_tag="JN701_001340"
     CDS             complement(9575..9790)
                     /gene="bdm"
                     /locus_tag="JN701_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAY7363845.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10131..10562
                     /gene="osmC"
                     /locus_tag="JN701_001341"
     CDS             10131..10562
                     /gene="osmC"
                     /locus_tag="JN701_001341"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY7363846.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(10618..11544)
                     /locus_tag="JN701_001342"
     CDS             complement(10618..11544)
                     /locus_tag="JN701_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7363847.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11537..12523)
                     /locus_tag="JN701_001343"
     CDS             complement(11537..12523)
                     /locus_tag="JN701_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7363848.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12520..13416)
                     /locus_tag="JN701_001344"
     CDS             complement(12520..13416)
                     /locus_tag="JN701_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY7363849.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13413..14435)
                     /locus_tag="JN701_001345"
     CDS             complement(13413..14435)
                     /locus_tag="JN701_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7363850.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14437..15987)
                     /locus_tag="JN701_001346"
     CDS             complement(14437..15987)
                     /locus_tag="JN701_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7363851.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(16001..16582)
                     /gene="ddpX"
                     /locus_tag="JN701_001347"
     CDS             complement(16001..16582)
                     /gene="ddpX"
                     /locus_tag="JN701_001347"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAY7363852.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(16840..19239)
                     /gene="dosP"
                     /locus_tag="JN701_001348"
                     /gene_synonym="pdeO"
     CDS             complement(16840..19239)
                     /gene="dosP"
                     /locus_tag="JN701_001348"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HAY7363853.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPDWMSSVLPLK
                     I"
     gene            complement(19264..20645)
                     /gene="dosC"
                     /locus_tag="JN701_001349"
                     /pseudo
     CDS             complement(19264..20645)
                     /gene="dosC"
                     /locus_tag="JN701_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(21017..22336)
                     /locus_tag="JN701_001350"
     CDS             complement(21017..22336)
                     /locus_tag="JN701_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY7363854.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYISIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22467..24002)
                     /gene="gadC"
                     /locus_tag="JN701_001351"
     CDS             complement(22467..24002)
                     /gene="gadC"
                     /locus_tag="JN701_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY7363855.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6012 a         6110 c         6119 g         5922 t
ORIGIN      
        1 acaatgagaa aaatagatga gataatatta tttatcgata tgtgatcgaa gtcgaaatga
       61 gatataaggt gaattactgg tatttgaaat ttatttttta atattgtcgg aatttatctg
      121 attaactgcc gggccgtaga cccggcaatt attttaacca cgacgtgtcg ccagccagca
      181 gagcagggaa ccgccgcaga ccattagcgc gccttgccag aatgagaacg acagcggggc
      241 gctgagcagc acggctgcaa gcgctgagga aagtacgggc gtaaaatacg agcctaccgc
      301 cattatggtg acattgccat gcaatatacc gacattccat gcagcataag caaatcctaa
      361 ggtaaatgcc gcagagatga gtttaatcat gacgggcgtg ctaaatacca tttctggttg
      421 ttgcgtaaga aaatagtaaa cccacagact tgctcccgtt agcaggacaa aaacggtaat
      481 tccgttaaat ccgcgtgcgt atttattcgt tactgtgcaa taggctgccc agataaacgc
      541 accaataaac gccaggaaat aactcaatgg gctggtggtg atattattga tgatttcatc
      601 ataatgtaac ccattctcac cgcctaacac ccagcagacg ccgaccaggg ctaataataa
      661 tcctggtaca atcagccagt tggttttctg accattaaac agaatggcaa agagaattgt
      721 caggctgggc cacagatagt tcaccatacc cgcttcaatc gcctgatgac gggtcgccgc
      781 ataccctaag gaaagcgcca gacagatttc atagctgacg aataacagac tcccggcgag
      841 taaatagcct ttcggaatct gccgaatacg cggaaatcca accgtgaaga ttaacagcag
      901 cccgcttaat gaatagatag cagctgcgcc gccgaccggg ccgagcccct cgctgacacc
      961 gcgaatcaat cctaccatcg tgctccacag gacgatcgct attagcccta tgagcgttgc
     1021 tttttgtcgt gtcatgctcg ctgttttgtc tctcttgccg ttaaaaatta agctgaattt
     1081 tatagcattt ttttaactgg cctgtcaggc agtggtgcgt ttttctaccg ctattgaggt
     1141 aggtcaattt gcgcaggcgg attattttgt ggcaaacacg tgttcttttt gatttcgcgc
     1201 aaaaagattc agaattttac tgttagtttc ctcgcgcagt aatatccctg aaaaaagagg
     1261 aaagcaatgg acgtcagtcg cagacaattt tttaaaatct gcgcgggcgg tatggctgga
     1321 acaacggtag cggcattggg ctttgcccct aagcaagcac tggctcaggc gcgaaactac
     1381 aaattattac gcgctaaaga gatccgtaac acctgcacat actgttccgt aggttgcggg
     1441 ctattgatgt atagcctggg tgatggcgcg aaaaacgcca gagaagcgat ttatcacatt
     1501 gaaggtgacc cggatcatcc ggtaagccgt ggtgcgctgt gcccaaaagg ggccggtttg
     1561 ctggattacg tcaacagcga aaaccgtctg cgctacccgg aatatcgtgc gccaggttct
     1621 gacaaatggc agcgcattag ctgggaagaa gcattctccc gtattgcaaa gctgatgaaa
     1681 gctgaccgtg acgctaactt tattgaaaag aacgagcagg gcgtaacggt aaaccgttgg
     1741 ctttctaccg gtatgctgtg tgcctccggt gccagcaacg aaaccgggat gctgacacag
     1801 aaatttgccc gctccctcgg gatgctggcg gtagacaacc aggcgcgcgt ctgacacgga
     1861 ccaacggtag caagtcttgc tccaacattt ggtcgcggtg cgatgaccaa ccactgggtg
     1921 gatatcaaaa acgctaacgt cgtaatggta atgggcggta acgctgctga agcgcatccc
     1981 gtcggtttcc gctgggcgat ggaagcgaaa aacaacaacg atgcaacctt gatcgctgtc
     2041 gatcctcgtt ttacgcgtac cgcttctgtg gcggatattt acgcacctat tcgttccggt
     2101 acggacatta cgttcctgtc tggcgttttg cgctacctga tcgaaaacaa caaaatcaac
     2161 gccgaatacg ttaaacatta caccaacgcc agcctgctgg tgcgtgatga ttttgctttc
     2221 gaagacggcc tgttcagcgg ctacgacgct gaaaaacgcc agtatgataa gtcgtcctgg
     2281 aactatcagt tcgatgaaaa cggctatgca aaacgcgatg aaacactgac tcatccgcgc
     2341 tgtgtgtgga acctgctgaa agcgcacgtt tcccgctaca cgccggacgt cgttgaaaac
     2401 atctgcggta cgccaaaagc cgacttcctg aaagtgtgtg aagtgctggc ctccaccagc
     2461 gcaccggatc gcacaaccac cttcctgtac gcgctgggct ggacgcagca caccgtgggt
     2521 gcgcagaaca tccgtactat ggcgatgatc cagttgctgc tcggtaacat gggtatggcc
     2581 ggtggcggcg tgaacgcatt gcgtggtcac tccaacattc aggggctgac tgacttaggt
     2641 ctgctctcta ccagcctgcc aggttatctg acgcttccgt cagaaaaaca ggttgatttg
     2701 cagtcgtatc tggaagcgaa cacgccgaaa gcgacgctgg ctggtcaggt gaactactgg
     2761 agcaactatc cgaagttctt cgttagcctg atgaaatctt tctatggcga tgccgcgcag
     2821 aaagagaaca actggggcta tgactggctg ccgaagtggg accagaccta cgacgtcatc
     2881 aagtatttca acatgatgga cgaaggcaaa gtcaccggtt atttctgcca gggctttaac
     2941 ccggttgcgt ccttcccgga caaaaacaaa gtggtgagct gcctgagcaa gctgaagtac
     3001 atggtggtta tcgatccgct ggtgactgaa acctctacct tctggcagaa ccacggcgag
     3061 tcgaacgatg tcgatccggc gtctattcag actgaagtat tccgtctgcc ttcgacctgc
     3121 tttgccgaag aagatggttc tatcgctaac tccggtcgct ggttgcagtg gcactggaaa
     3181 ggtcaggacg cgccgggcga agcgcgtaac gacggcgaaa ttctggcggg tatctaccat
     3241 catctgcgcg agctgtacca ggccgaaggt ggtaaaggcg tagaaccgct gatgaagatg
     3301 agctggaact acaagcagcc gcacgaaccg caatctgacg aagtggctaa agagaacaac
     3361 ggctacgcgc tggaagatct ctatgacgct aatggcgtgc ttattgcgaa gaaaggtcag
     3421 ttgctgagta gctttgcgca tctgcgtgat gacggtacaa ccgcatcttc ttgctggatc
     3481 tacaccggta gctggacaga gcagggcaac cagatggcta accgcgataa ctccgacccg
     3541 tccggtctgg ggaatacgct gggatgggcc tgggcgtggc cgctcaaccg tcgcgtgctg
     3601 tacaaccgtg cttcggcgga tatcaacggt aaaccgtggg atccgaaacg gatgctgatc
     3661 cagtggaacg gcagcaagtg gacgggtaac gatattcctg acttcggcaa tgccgcaccg
     3721 ggtacgccaa ccgggccgtt tatcatgcag ccggaaggga tgggacgcct gtttgccatc
     3781 aacaaaatgg cggaaggtcc gttcccggaa cactacgagc cgattgaaac gccgctgggc
     3841 actaacccgc tgcatccgaa cgtggtgtct aacccggttg ttcgtctgta tgaacaagac
     3901 gcgctgcgga tgggtaaaaa agagcagttc ccgtatgtgg gtacgaccta tcgtctgacc
     3961 gagcacttcc acacctggac caagcacgca ttgctcaacg caattgctca gccggaacag
     4021 tttgtggaaa tcagcgaaac gctggcggcg gcgaaaggca ttaataatgg cgagcgtgtc
     4081 actgtctcca gcaagcgtgg ctttatccgc gcggtggctg tggtaacgcg tcgtctgaaa
     4141 ccgctgaatg taaatggtca gcaggttgaa acggtgggta ttccaatcca ctggggcttt
     4201 gagggtgtcg cgcgtaaagg ttatatcgct aacactctga cgccgaatgt cggtgatgca
     4261 aactcgcaaa cgccggaata taaagcgttc ttagtcaaca tcgagaaggc gtaagggggc
     4321 gaacagatgg ctatggaaac gcaggacatt atcaaaaggt ccgcaactaa ctccatcacg
     4381 ccgccttctc aggtgcgtga ttacaaagca gaagtcgcaa aacttatcga cgtttccacc
     4441 tgtatcggct gtaaagcctg tcaggtggcg tgttcggagt ggaacgacat ccgtgatgaa
     4501 gtggggcact gcgtcggggt ttacgataac cccgccgatc tgagcgccaa gtcctggacg
     4561 gtgatgcgct ttagcgaaac cgaacagaac ggcaagctgg agtggctgat ccgtaaagac
     4621 ggctgtatgc actgtgaaga tcccggctgc ctgaaggcgt gcccgtctgc tggtgcaatc
     4681 attcagtacg ctaacgggat tgtcgatttc cagtcggaaa actgcatcgg ctgtggttac
     4741 tgcattgccg ggtgtccgtt taatattccg cgcctcaaca aagaggataa ccgggtatat
     4801 aaatgcacgc tctgcgtcga tcgcgtcagc gtcggccagg aaccggcttg tgtgaaaacc
     4861 tgtccgaccg gggctatcca cttcggcacc aagaaggaga tgctggagct ggcggaacag
     4921 cgcgtggcga aactgaaagc gcgtggttac gaacatgctg gcgtctacaa cccggaaggg
     4981 gtcggtggta cgcacgttat gtacgtgctg catcacgccg atcagccgga gctgtatcac
     5041 ggtctgccga aagatccgaa gatcgacacc tcggtaagcc tgtggaaagg cgcgttgaaa
     5101 ccgctggcag cggctggctt tattgccact tttgccgggt tgattttcca ctacatcggt
     5161 attggcccga ataaggaagt ggacgatgac gaggaggatc atcatgagta agtcgaaaat
     5221 gattgtgcgc accaaattta ttgatcgcgt ctgtcactgg accgtggtga tttgcttctt
     5281 cctggtggcg ctgtccggga tttcgttctt cttcccgacg ctgcaatggc tgacgcaaac
     5341 cttcggtacg ccgcagatgg gacgcatttt gcacccgttc ttcggcattg cgattttcgt
     5401 cgcgctgatg tttatgtttg tgcgttttgt gcatcacaac atcccggata agaaagatat
     5461 tccgtggcta ttgaacattg tcgaagtatt aaaaggcaat gagcacaaag tggcggatgt
     5521 tggtaagtac aacgccgggc aaaagatgat gttctggtcg atcatgagca tgattttcgt
     5581 gctgttggtg accggggtga ttatctggcg tccgtacttt gcgcagtact tcccgatgca
     5641 ggttgttcgc tacagcctgc tgatccacgc ggctgccggt atcatcctga tccacgccat
     5701 cctgatccat atgtatatgg cattctgggt gaaaggatcg attaaaggga tgatcgaagg
     5761 gaaggtaagc cgtcgctggg cgaagaaaca ccatccgcgc tggtatcgtg aaatcgagaa
     5821 ggcagaagcg aaaaaagaga gtgaagaagg gatataatct cttttgaact ttaagctgaa
     5881 aatggcgctt taagaagcgc cattttttta ttgcgtaacc agacggcgca gtcgcgacac
     5941 atccaccgtt ttttcagcct ctgccagact ccaggcgttt tgcagattca gccacatttg
     6001 cggcgaactg ccgatcacca cggaaagttt aattgccatt tctggcgtca aagctgcttt
     6061 tccggtcagc aatcggcttg ccgttgaggg cgcaatttcc attgctctgg caaactcgcg
     6121 caggctgaca ttaagttcgt ccagtgattc ctgaataatg tcccccgggc ggggatgatt
     6181 ggccattttc attagtgtct ttactctcag tagcgttcct tctctgggaa cactatcatt
     6241 attgttcctt cctacgcaac aataatccat ttagccattt catgttggag agtgtgcgat
     6301 gctgcgaccc gaacatggga gtcgcagcaa agacctctta gtgacggaaa tcaatcacca
     6361 tacggccacg gattttgcct tcttccatct cggtaaagat ggtgttgatg tccgctaacg
     6421 gacgcagggc gactttcggc accactttgc cttcggcggc aaactggaag gcttcggtta
     6481 gatcctggcg cgtgccgacc agcgaaccga ccacttcaat gccatccagc acaagacgtg
     6541 ggatatccag gctcatagac tccggcggca gaccgacagc cacaacacga ccgcctgcac
     6601 ggacagcatc aactgccgag ttaaacgcag ctttagctac tgctgttacc accgcagcgt
     6661 gagcgccacc ggttttctcc tgcacaattt aggcggcgtc ttcggtgcgt gagttaatcg
     6721 ctaaatctgc acccatttcg gttgccagtt ttaactgctc atcattgaca tcaatggcga
     6781 tcactttggc gttaaagaca ttcttcgcgt attgcagggc gaggttaccc agaccgccaa
     6841 gaccgtagat agcaatccac tgaccgggac gaatttttga cagtttaacg gctttgtagg
     6901 tggtgacccc cgcacaggta atgctgctgg ccgccgccga gtccagacca tctggcactt
     6961 ttaccgcgta atcggcgacc acgatgcact cttccgccat cccgccatca acgctgtatc
     7021 cggcattttt aactgaacgg cagagcgttt cgttaccact gttacagtat tcgcaatgac
     7081 cgcatccttc gtaaaaccac gccacgctgg cacgatcgcc tggttttaat gaggtgacac
     7141 ctgggccaac ttctgccacc acaccgatgc cttcatggcc cagaattacc ccagttttgt
     7201 caccaaaatc gccattctta acatgaagat cggtatgaca tacaccacaa cactccattt
     7261 tcagcagggc ttcgccatgt ttcagtgagc gcagtgtttt atccgtaacg tcaacatgat
     7321 gatccttcgt aacaactgca gccttcatag ttcctccttt tcggatgatg ttctgcatag
     7381 caggtgaggc aaatgagatt tattcgccac tacccagtat ggatgagatc tgaaaaaggg
     7441 agagggaaat agcccggtag ccttcactac cgggcgcagg cttagatgga ggtacggcgg
     7501 tagtcgcggt attcggcttg ccagaaatta tcgtcaatgg cttgttgcaa agcttcagca
     7561 gacgttttca ccgccacgcc ttgctgctgc gccattttgc caaccgcaaa cgcaattgcg
     7621 cgggagactt tctgaatatc tttcagttcc ggtagtacca gaccttcgcc gttcagcacc
     7681 agcggcgaat actgagcaag cgtttcactt gctgacatca gcatctcatc ggtgatacgt
     7741 gacgcgccgg aagcaataac acccagcccg atgccaggga aaataaaggc gttgttacac
     7801 tgggcgatag ggtagatttt atctttccat accactggat taaacgggct gccagtggcg
     7861 accagcgcgt taccttcggt ccaggcgata atgtcctgcg gtgtggcttc cacgcgtgac
     7921 gtcgggttag acagcggcat cacgatcgga cgcggacagt gtttatgcat ctcacggatg
     7981 atctcttccg taaacagccc ggtctgtcct gagacgccaa tcagaatatc tggttttaca
     8041 ttgcgcacca catccagcag tgacagcaca tcgctgtcgg tatcccagtc actgaggttt
     8101 tcgcgtttct gcaccagttt ggtctggaaa ggcagcaggt tcggcatctt gtccgtcagc
     8161 aggccaaagc gatcgaccat aaagactttc tgccgcgccg cttcctcgct taatccttcg
     8221 cgctgggtct gggcgatgat catttcggca atgccgcatc ccgctgaacc tgcaccaagg
     8281 aagacgattt ttttctcgct taactgacca cctgccgctc ggcttgctgc gatcagtgtg
     8341 ccgactgtta ccgccgcagt gccctgaatg tcatcgttaa aagaacaaat ttcattgcga
     8401 tagcggttaa gtaacggcat cgcatttttt tgagcaaagt cttcaaactg caacagcacg
     8461 tctggccagc gttgtttcac agcctggata aattcatcaa cgaattcata gtattcgtcg
     8521 tcaatgatac gcggattacg ccagcccata tacagcggat cgttaagcag ctgttggttg
     8581 ttcgttccga catccagcac caccggaagg gtatacgccg ggctgatgcc gccacaggcg
     8641 gtatagagcg acagtttacc gatcggaatg cccatcccgc cgatgccctg gtcaccaagc
     8701 cccagaatac gttcaccgtc agtcaccaca atcactttga tattatggtt cggtacgttt
     8761 tgcagaatat cgtccatatt gtggcggttc tggtaagaga taaacacgcc gcgtgaacgg
     8821 cggtagatct cagaaaaacg ctcacaggct gcgccgacgg ttggggtata aataacaggc
     8881 atcatctcat caagatgatt gtttaccaga cggtagaaga gggtttcgtt ggtgtcctgg
     8941 atgttacgca ggtagatgtg tttgtcgatt tcggttttga atccctgata ctggatccat
     9001 gctcgttccg cttgttcttc gatggtttcg accacttccg gcagtaaccc cagcaggttg
     9061 aagttacggc gttcttccat gctgaaggca ctgcctttat tcaacaacgg aaattccagc
     9121 agtacagggc cagcgtaagg gatataaagc gaacgctgtt tttttgtttt tggttccatg
     9181 tcactcactc ttttttgaat atccatccct ggcacgcttt tatcgtcttt gctttaccgc
     9241 cagggcgtcg gccccaagta taaagcagat aaaaacaaaa acaccattgc gcaggcaatg
     9301 gtgtttaatc gtcattgagg actgatggtt atgaattact tttcagcggg gcgttttctg
     9361 ccggttgggt tatttaccac gctggatttg tcaccttcgg taactatttt gcgctggtta
     9421 gaaattttat ggtccagtcc aagaatatga cgtgcctgac ggttcgattt cattttaact
     9481 cctcaatcct gttgctagtt ttaaggacaa catcgccgta gcgaagaaac acgtgctaaa
     9541 cccctaaatt aggttgccga tcaagcatag caccttaaag cgtagggtgc tggccactga
     9601 ccacataatt gatcgtttgc tggtagatat cactgaggat gtcgttatca gaagcttcca
     9661 cccatttggt cagctccatg agaatgtcat cttcagtgac aacaccgtgc tctgcccgaa
     9721 ggccttgctc aatggcatta accagagcgg gttctgctgt tgaattttct gcctgataat
     9781 aagtaaacat agtgattctc cgtgtctgtg tatttatggt gtctgctacg gatcgcagat
     9841 ttataaagca cattcagcat ggcaaatatt tgccgctttg tagattagtc ctggttgatg
     9901 atttttatat tttaacctca tgatattgat aggtattatt attgtggtga gttgattaat
     9961 attaatgcaa tatctcttgg gttatatatt cccggttatc tattgtggaa atttaattta
    10021 agtgcagaag taatatttct gccggatttt tattcggaat atcctgctta tcctcgtgct
    10081 gtttctcacg tagtctataa tttccttttt aagcccacat gagagcaaca atgacaatcc
    10141 ataagaaagg tcaggcacac tgggaaggcg atatcaaacg cgggaaggga acagtatcca
    10201 ccgagagtgg cgtgctgaac caacagccgt atggatttaa cacgcgtttt gaaggcgaaa
    10261 aaggaactaa ccctgaagaa ctgattggcg cagcgcatgc cgcatgtttc tcaatggcgc
    10321 tttcattcat gctgggggaa gcgggattta cgccaacatc gattgatacc atcgccgatg
    10381 tgtcgctgga taaagtggat gccggttttg cgattacgaa aatcgcactg aagagtgaag
    10441 ttgcggtgcc gggtattgat gcctctacct ttgacggcat catccagaaa gcaaaagcag
    10501 gatgcccggt ctctcaggta ctgaaagcgg aaattacgct ggattaccag ttgaaatcgt
    10561 aaagcattgc cggatgacgt gtcaggcgcg tgaatgcctg atgcgttgtt agcatctcta
    10621 cagcgcccgc cagcaccgga gctcacgtcc gtcctccctt attgctaatg attgccggac
    10681 ttcacatccg ctggttgcaa gggggcaacg ttcgtagaaa aaacagcctt gcggcaacgt
    10741 gcggtttccc ggcagatccg ttttacgtaa tgcccattct tcctccagcg gtttatcaat
    10801 ggcggggagg gaatccagca ataatcgggt gtatggatgt gccggagccg ttagcacctg
    10861 ttgcgcgtcg cccagttcta caatctgccc gagatacatc accgccaccc gatcgctcat
    10921 atgacgtatc accgagacat tgtgtgaaat cagcacatag gtcagcccgt gattttcctg
    10981 tagcgttacc agtaaattga ggatctgcgc ctgcacggag atatccagcg cagaggttgg
    11041 ctcatcaagc acaatcacgt caggctgcga agagagtgct ctggcaatgg cgatacgttg
    11101 ccgctgcccg ccggagaacg catgaggcag gcggtcgaga tactccggac gaatacccac
    11161 ctgcacagcc agctcctcta ccaacgctcg ccgctgttgt tcactactac gcttagctat
    11221 ccagagcggt tctgtgatga tgcgccacac cggtaagcgc ggattgagcg aagagagcgg
    11281 gtcctgaaac accatctgca taatgcgttg tgagccagaa cggatgtact gcccgtggct
    11341 cggttgcagc atacccatta aaagctgtgc gagggtgctt ttgccgcagc ctgactcgcc
    11401 gacgatccct aaggtttcac cacggcggat ctgtaaatca ataccattaa tcgcatgaac
    11461 atgttccgta gttttaccaa gccagttttt acgggccggg aaattgatat ggacgccgcg
    11521 taacgttaat aacgtgtcag acacttatga cctcctgctg cggataccaa caggcgcagc
    11581 gctggttgtt gtcaccacac gctgtcagcg ccgggacgtt ttcacactgt gcacctgccg
    11641 catagcaacg atcgcgaaaa gcgcagccgt caggcaaatg ggtgaggttt ggtaccgtcc
    11701 ctggaatggc gggtaatagc tggcgtggta ctccatgttc tggtgcacat tggagcaaac
    11761 caatggtata cggatgccgg ggatgatgga taacgtctgc cctcacgccg ctttcaatta
    11821 cgcttccggc atacatcacg taaacgctat cgcacagttg cgacaccacg gccatatcat
    11881 ggctgatgaa cagaaccgca gttccactgg cgcgggcttt atgtttaagc aaacgcagta
    11941 cctgcaactg caccgtgacg tccagcgccg tagtcggttc gtcggcaata atcaattgcg
    12001 gctcgcagga gaatgccagc gcaatcatta cccgctggcg cataccacct gaaagctcaa
    12061 acggatagcg cgacataact tccacggcat ccgggatttg catctcttcc agcaggacaa
    12121 tcgctttagc tctggcttcc cgacgactta ttggttgatg atggcggatc acgtccatca
    12181 tctgaagacc tattcgacgt gtcggattga gggcggtcat cggttcctga aagatcatcg
    12241 ccactcgtgc gccgcgccac tgacgaagct gtttttcccg agcgttaaga acatcgtctc
    12301 ccagcagtga aatctgcccc cgatgtacgc aataactgcc cgtcggtagc agacgcataa
    12361 tcagcattgc ggtgactgat ttacctgagc cggattctcc caccagaccg acaatttcac
    12421 cgcggttaat ctgcaaggac acattgttga gcgcgtgaac atcaccgtta aaaccgggga
    12481 aactcaaatg cagctgttga atgtccagaa cgggctgggt catgactgct ttcctcctgc
    12541 tttcgggtca agcagatcgc gaataccatc accaaagaga ttaaacccaa cggcggtaag
    12601 caaaatcgct gcccctggaa atgcgcaata ccaccactga tcgagtacat agttgcgacc
    12661 attcgctacc atcgcgcccc attcagcact cggttgttga gcacccaggc caataaatcc
    12721 caacgtggcg gccattaaaa tcgcgctacc gatatccagc gatgcctgca cgatcagcgg
    12781 cggcagggag ttacgtaaaa tatgccagct gatcagatgc caacgcgacg caccaaaggt
    12841 tttcgccgcc tgaacatagg tatactggcg tacaactaat gcttgccccc gcgccaggcg
    12901 cacataaaag ggaattcgca caatagcaat cgccagcatg gcgttaaata aactcggccc
    12961 gagagcggca gccagtgcca ttgtcagtac caacgaagga atcgacagca taatgtccat
    13021 gatgcgcatg atgatggcgt ctgcgcgtcc gccaagcaca ccggatagac atccgagtag
    13081 cgaaccaatc atacccgcaa tggcaaccac gactaatccg gcgagaattg attgctgact
    13141 gccgaccagt acgcggctga acagatcgcg tcccacttca tcggtgccaa accagtgcgc
    13201 cgcagaaggc ggcaaaaggc gggcggttaa atcaatggcg ttgggatcat gcgtcgcaag
    13261 ccacggtgaa aaaaacatca tcaatagcat taatacaatg atgactgcgc cggtcacggt
    13321 gagcggactg cctttcagca tccataccag ttttgcaccg ttaaatcgcg tttgtttttg
    13381 tgggcgtacg gcggacgttt cctcgcttag catcattcac cacctccacg tccgatacgc
    13441 ggatcaatcc acaaatagag caaatccacc accaggttga ccagcacata agcaaatgaa
    13501 accacgacgg caaagcccat cactgccggg aagtcgagcg cctgtattga tgttactacc
    13561 catgcaccca ttccgggcca ggcaaaaacg gtttcggtga gcactgcgcc atacaacaaa
    13621 tcgcccagcg ccagacccaa tacggtaatc gatgggatca acgcattggg tagcgcataa
    13681 cataaaacga tataccagcc gggcaagccg ctggcccggg cggtacgaat gtagtcttca
    13741 ctcaattgtt ccagcatcgc tgagcggatc tggcgagcga caattcccag gtgaacgaac
    13801 gccagcgtta atgccggtaa gatgagatgt tgcaacgcat tgaagaagac ttcaccgttg
    13861 ccttcaagca gcgcatcgag cagataaaag ccggtaacgt gcgttggtgg atccagccag
    13921 tcatcaagcc ttccgccgcc gggaagaatt tgcagatgac cataaaacag cacaatgacg
    13981 cccagtccaa gccaaaacgc aggtgtggag attccggtaa tggccattat tcgcaccaga
    14041 tgatccagcc agcgatttcg ccagactgca gagagtatgc ccagcgggat gccaatcagg
    14101 agtgccagca gcagggcaca aaaagccagt tccagcgtcg ccgggaaaaa tatacgcaac
    14161 tcttccaaca ccggacgccc ggtacgaatg gatgttccca ggtcaccatg aaacaggtcg
    14221 ctgacgtagc ggtaaaactg tacatacagt ggctggtcca gtcccagttg ctggcgaata
    14281 ttttccacga tagcatcgct ggcgcggtca cccgccagta accgcgccgg atcgccaggg
    14341 atcagatgcg agataataaa cgtaatcacg cagacgcccg ccatcacgag caccagcccc
    14401 cagcagcgtt ggcgtaaaat actccagaag gtcacgttat ttactcatgg tattgatatt
    14461 gaagacctgt tccagcatgg gattgaacac aaagcctttc acctctttgt tcatcgccag
    14521 ttggtagttt ttctggaaca ggtacacata agcagcgtca tcaatgacga ttttctgtgc
    14581 ctgctggtag tcccgggtac gctgcgtctg gtcggtggtc gcaagcgcat tgcgcagtaa
    14641 cttatcgacc tcactgtttt catagaacga gcggttaccc ggcagacctt ttttgtctga
    14701 ctcaaaccag taattcataa acatatacgg gtcggcaaaa tccggactcc agttgccaat
    14761 cgcaatgtcg taatcacctt tacccactct gtcgcgcatg gtggcgttcg ccagcttttc
    14821 cagcttcaca ttgatgccca gcttgttgag actggattgt gtcgccagag caataggctc
    14881 ccagttcgga tccttatcgg agtagagaaa cgtcaggctg gtgggtttgc tcgtcacttt
    14941 atcccattcg gctttggctt tcgtttcgtc atggttgtat tgcattgccg tcgcatcgta
    15001 gccccacatg ccttccggaa tcgggccgcg catctgtttt ccgttaccac tcagaatgcc
    15061 gttaaccatg ccctgataat cggtagacca ggaaatggcc cgacgcagat ccgcctgatt
    15121 aagaggcgct ttgctgttat tcagatacag gtaggtgacg cgcagtgacg gatactctgc
    15181 cacattgact ttgttttcct gcttcagggc gttgagttga tccaccggca gcgcatcggc
    15241 aatgtcaata tcaccacggg agagctgcag gcgacgggag gcactttcac caataatttt
    15301 taccgatact cgcttaaagt tcggtttatt gccagggtaa tgcggatttg gcaccagaac
    15361 taattgctga cctttttgcc agcttttcag cataaatggt ccggaaccgg cggtattttg
    15421 cgcgaggaag ccgcgggcat catccgctgc atgttccttt aacaccgccg gattgataat
    15481 ggatgcaccg tcattcgcca gcgtgtagag gaacggtgcg aatggctggc taagggtaaa
    15541 cttcaccgta tgttcgtcgg gagcatcaat ctttaaatct ttgggaaatg cttctgctgg
    15601 cccctggccg atttttagta gccgctcaaa agagagtttt actgcttcgg cagtgaccgg
    15661 tgtgccatcg gcaaatttag cattgtcctt cagggtgaac gtccactctt tttgatcgtc
    15721 agacgctttc cagctacttg ccagatcgcc ttcaacgtcg gttgagcctt tatcaccgtc
    15781 cgttttgtac tgaaccagcc gctgataaga cgggtaggtc accgtccagt cgttattatc
    15841 tattgttacc gccggggcga gggtttgtgg atcggcggcc ttaccaatca ccagcatatc
    15901 tttcggtacg gcggcgtgcg caaccgggaa agttgtggca aggacgagcg cgagcaatgt
    15961 aggacgaaac ggtatcgatc tcttcatgac agtttcctga ttaactgacg tgctgtgtgc
    16021 caggggatat aaaacaggtg aattgatcag cgagcagtgg gtaactcgct gcctggggta
    16081 attcgaagtg ccaccattcg ctggagatac cgacaaaacc gccaccagtc ataatcgcat
    16141 tcagcaacag ccgattgcgc tgagcggcgg gcgggacgga agggtgatag gcatgggagc
    16201 gctcgtgcat ttcatcgaac cctgcgccca tatcgaggat gttcccgtgc tcatcacgaa
    16261 gcgtcaggtc gatcgccgtg ccacggctgt gattagaacc gaccgtcaca tcaacaacat
    16321 attgcgggtc tgggcaggct tgccacaaca tcgcctgtgc ttgttgtggg cgatacgcat
    16381 cgtaaatcac cagttgtaag cctgacagct gggcgatact gatacttttc gccagcgcgg
    16441 taatcgcatc cttgtgtagc agacaacgcg cttgctgata aatagcttta cctgtgatgt
    16501 tatcagcgca ggcgtatttc aattcgatct ccagatcagg gaagattacg gctaaatcaa
    16561 ccagttcggt ggtatccgac ataaacggtt cctgtataag acaaaaattg ctgcgctttc
    16621 cgcttatgca gatctcatgc catgccggga taagcgccag aatgctggct taaagttata
    16681 ttttttaact tttgatcaac atttgtgcag cgtagtgcag ttttggtgca agaggggaag
    16741 ttaaggaagg aatctcccgg aatcgtagat gaaatcacag tatttaagtg acggtgtcac
    16801 gttaaatgaa aacccgcgag tgcgggcgag aggaatttgt cagattttca gcggtaacac
    16861 gctgctcatc cagtctggaa tttcttcggc gggtaggggg cgggaaaaga aatatccctg
    16921 aataacgcga cagtggatct tgcgtagcat ctcaaattgc tctttggttt cgacgccttc
    16981 cgccacgacg gttaaattga ggctttgccc aatgctggta atggcttcaa gtaaggcaag
    17041 gatgcgtttt tcggtcagac aacgatcgac aaaacttttg tcaattttga tttccgttac
    17101 cggaagactg actaagcggg ataatccgga aaagcccgta ccaaaatcat ctaccgataa
    17161 gcccacgccc atatcacgca ggatctgaat gcgcttaaag atttcggtat cgtgttccat
    17221 catcatgctt tccgtgattt ctaccgtcag ctggtggccg tcaatacccc aggcgtgcat
    17281 tgcatcagac acctgattag gcagttgatt actgcgaaag tgcagtgccg acaagttaac
    17341 ggaaagcgcc gggatatgaa tattctggct acgccattct gctaactgac ggcaagcttc
    17401 cgcgatgacc cagcgcccaa tattttcgat ttcaccaatc tcttctgcga gaggaataaa
    17461 ccgtgaaggg ggcacatgac catgctgggg atcgtgccag cgcgcaaggg cttcgatgcc
    17521 gtacagttca cccgtttctg cgaagatttg cggctggtaa acaagtttca gttggttatt
    17581 gctaatcgct tctttcagcg ctgcgcctaa aaccaaacgc tcttttacca tttcgttcat
    17641 cgccgggctg aagaactgcc agccgttacc gccattcttg cgaatataat ctattgcatt
    17701 gtgagcagtg gagagtaggt aatcgtggtt tttacccacg tcgtagctga tgccaatact
    17761 caaggtaagc gggaatggtt tatcgtcaat cattatcggc ttgctgacca cattccgtag
    17821 ctcatcggcg atctgggtaa tgttactgac gtcgttttcc aggctcacga ggacaaactg
    17881 cgtaccttcg atacgacaga gatactgatc cggtttgagt tttccacgaa agcgattgac
    17941 cacttccagc aatgcctgat cggcccacgc atagccaagg ctatcaatca catcctgaat
    18001 atggtcaaca ccgatgagat acaccaccgg agagacggct ttgtcgacca ggtcatcgag
    18061 gtaattgtgc aggttattgc gatttggcag accggtcatc ggatcaaatt ggatgagttg
    18121 ttcaatatgc tggcggcttt tttcctgttc cagcgccagc gcggccatat gctgactgat
    18181 atctgccacg cgttcgataa aggcgctggt ttctgctcct gacgaggttt taatttgcag
    18241 gatccccgca ggcgcgccat cacgctgacg aatggtcgct gaccagcttt gcgcattttg
    18301 aacttctgca ccgtgcgaag atgacgccca gtgtatcggc atcccgttgc gcagtgcgaa
    18361 cagcgaaaca tgcgattcgt tgagaacaga ttcgatgtta cgacaaatga tttcccccat
    18421 ttcatgaaat ggcgggctgc tgcacatggc ggcgagaata ttgccttcaa gctggcggat
    18481 ttgccgttct tcggtgatat ccgagaaagt cattaccagg ttctgcagat gcgcgagcac
    18541 gtcataaacc gggctgatag aggctttaat ccagattttt tcaccggtgc gcgtcaacag
    18601 cagaaattcg tcctgatcgc gggcggtttt ccatagcaac tgttgtaaac gaatgcggtt
    18661 atcggcaggg aattcaggaa tgttcaggag tgtatcgggc tgcataccgc tggcttcgct
    18721 aatgcagtaa ccaaacattt cggtaaatgc gcgattgcac tgcacaatat ggcgttccgg
    18781 atcgaggaca atcaccggtc ggtcgagatg gtcaacggca ataatcaatt gtcgggtctg
    18841 ttctttttgc gccatttcta cgctggcatc ccgtaccagc gccaggtaat aaactttccc
    18901 ctcggcgctc actttcgata gcgcaaaacg ggtccagatt ttactgccgt cttttttctc
    18961 cagctgcagc tcccgactca tcccctcgac gcgcgcttta ccaccttcac ggttatgacg
    19021 aatgtattca ggatgcgcag gacgcaaatc ccgcggaatc agcatatcaa tgttattgcc
    19081 aatgacttct tcacgtttgt acccccagag cttctctgcg gcggggttga aaaacatcac
    19141 ttcatcattt tcgttaatta acaccgcgcc catcatattt tgttcaaggg cggggaaaaa
    19201 aatgccatcg gcggcatttt ccgcatcggt tagcttcatg attacctctg catcctggcg
    19261 catctaaaga ctggctttcc agagttcaac acggtttcta cctcgtcttt tggcgatata
    19321 cagagcttca tcggctattt gaatgaggcg ctcatagtca ggatgaccat taaacatggc
    19381 ggcaccgatg gaaagtgaga gggcaatatc ctcgccgttt gcggctttca gtttggtttt
    19441 ctccacccga ctgcgaatac gttctgcggt acgtaacgtt tcgttttcag aagcttcagt
    19501 caaaacaatg ataaattcat cgcccccgta gcgaaaaaca taatcactac tgcggacgtt
    19561 gtcataaaag gcctgagaga ctttacgcag aatttcatcg ccagtgttat ggccccacgt
    19621 atcgttgatc tctttgaatt tatcaacgtc aataatcagc actgacagcg gtgtaccggt
    19681 ccggttggca tgggcaattt cgcgtttgaa gatagtcggc aggaagcggc ggttaagtaa
    19741 tttcgtcagt acatccatac cgacttcgtg gcgcgatact tcttcaaaca attcacgcag
    19801 caaggtaata atttgcgata cagtatttct tatctgtaat aaaaatttca cccgcagact
    19861 tctgttattc aaattcctgg tgttacgcat ggtttgattg aatataccgt cgaaatcctg
    19921 aatcagacgg gagatatgcc tacttcggca atgccactaa aataatgtcg acctttatgg
    19981 ttaaaccaca ggccaaaatc agcctggctt aaaggcaaac tactgcctaa atcagaatcc
    20041 agcaggattt tatagataat atctatttcc catgaaagta ttgaggctat ttgccgttct
    20101 ttttcttctt cggcgttttc tagtaacgag aagatacgat agttttcatc ttcctttgag
    20161 gcactactgt cactaaaggt aaacgcgcgg gtcatcactt ccatagcgat atcaatactg
    20221 ttaatcgaga aatggtagac ctgaagtttt tctgcggcag aataatccga agagaagatt
    20281 accggataga ggattttttt cagcacccga aaccccatct cgacaatttc taccggaatt
    20341 cctatgcggg catgcacttc cgcgacggta tgctggattt gtattagcct ttcgacatcg
    20401 tcaacctggg cagaaagcac gttaataatc cagcgttcca tcgcactttt caactgccgc
    20461 tcaacttgtt cattactcaa gaattcttcg gcatgcgggt cgatgcggac aattcgataa
    20521 aactcgatac tcagataatg agcatgcgca acggcaattt ccgcggcttt agcacgaatg
    20581 agtggatctg cctgttcgac aagtccggtc cactcatctt tcattctttt aaaatacatc
    20641 tccataattc acacccttat aaggctggga aatcagacgg aatcaaaacg caacgtgcga
    20701 gatcgactaa ctgcaccata ttctcctgaa atatgaagat atactgaaaa gaaataagcg
    20761 acttaggaca gtttcaatct acgctactgt tcttcagaag agtatagccc atcgtaatta
    20821 tttttcggtg acagcgaata tcgtatggtt tttcatattc atacattttt attagggatt
    20881 tatagctgtt taactaagtg tggttaattg cgtggttaat ttgacttatg taagcatgat
    20941 tactagtggg atatttcaca aggtgtaaca agccggtggg taaagcaccg gcttgttaca
    21001 aagtaagcat gggagtttaa ctgccccaac gactttgcag atagctgacc gcttgttgag
    21061 tctgcggttt attcagatag tcctcacgga acaagatggt gccgctaatt tctggtaccg
    21121 catcgttaag atcgagctgc tttttcagtt ccggtacgcc gccgttaatc atccagtctg
    21181 gctctatctt tgaaggttca cccactttat agaaggcgat actgatatac aggcgggtcc
    21241 tggtcggttt aacgacatcc gcccaccatt ttgccaacac gtcataacgc gcggcactcc
    21301 gtgagaacgg ccagtaaatt tggggagcaa tgtaatccag caatccttgt tccacccatc
    21361 gacgggtgtc agcgtaggat tcgtcatagg ctgccgcgcc tcgggtatcg gaaccgagcg
    21421 gatcgtgtga tcggttacgc cacacgcctg ccgggctaac gccaaattcg actcccggct
    21481 taatgctttt aatggtgtgc gatacttttg taattaactg ctgagtattg ttgcgccgcc
    21541 agtctgcttt tgacgcaaat gcgcctccgt atttacggta cgtttcgtta tcatttagcc
    21601 gtgaacccgg tgactccgta tagaaatagt cgtcaaactg cacgccatct accggatagc
    21661 gggaaaccac ttcggcgact attgatgtga tccagtcctg aacctcaggg atgcccgggt
    21721 cgaggacaaa gcgatcgcca gacgttctga tccagtcacg gtgttgcaca tagacgctcg
    21781 ccggttgttg agacagagtg ctattcagtt ccctgatagt accgggcttc gtattaaccg
    21841 atacgcgata ggggttaaac caggcgtgta ctttcatccc acgcttgtgg gcttcgtcga
    21901 gcataaattg cagcggatcg taacccggat tttcaccaat cttaccggtc ataagatcgg
    21961 accacggcaa aatttttgat ggccacaggg cggtaccgtc cggcttgacc tggaaaaaga
    22021 ccgtgtttat accgagacgt tgcagatgat ccagtttgtc gatcatcgcc tgttgttgta
    22081 cacgggcccg gctggtgggg ttactaatgt taaccgagga aaccggtggc cagtcgaggc
    22141 gagacaccgt ggccagccag atgccacgca tcggttgtga cgattgttgc gtcgtggctg
    22201 gtggctttga acctgctggt ggtgtcacca tggactctgg aggcgtgctt ttacaactac
    22261 acagtaaaag tgccagtgca accagtatcg ctggtcttct aatcgttaat ttcttgtttc
    22321 gggagcagat atccatatgt gctggtttcc ggtaaacaga tgtgcgctca ttctcgtact
    22381 tatcccggtt aagtcaatac gacagcaagc acgaaaaagg gagcgatgaa ttatcgctcc
    22441 cttgtcttat aaccattcag acatggttag tgtttcttgt cattcatcac aatatagtgt
    22501 ggtgaacgtg cacgcgggtg caggaagaag tgaccttttg gtgcgttctg actgttgatt
    22561 ggctccagag tgacgccggt attcgctttg cctttacgat catgaacagc atagagaata
    22621 aagggcaggg caagtaccac caggaaacta acgaccagta attcaacata catatcggta
    22681 gagtcaccct ggatgttatc cggcggcagg aaggaaacaa taaacgccat aattgaagtc
    22741 agcagaccga caattgccac gaccagtttc acccctttac caccagggat attaaatgtg
    22801 cgttttaagt caggatgttt aagaaccaac acaatgtagc caataaacag catgaaataa
    22861 gcacacagat aaatcaccac cgtcagcgcc agtgcgatca ggaaggacat gttgttaccg
    22921 ccaccggtat tggtgaggat gatcaacgcg atagaagtaa tcaccagctg cgaaatgacc
    22981 agcgttaccg gtacgccatt tttgttcatt ttagcgaatg ccgctggcag caggtttttc
    23041 tgcgctgtga catacatccc gcgagaagga ccaacaatcc aggaggcgat ttccgccaga
    23101 acacccagca acagcagtgc ggagatcacg cgaaccgtcc actcaatttc cggtgccaca
    23161 tgggacatca gaacggtaaa ggtttgcatt acccctgcgg agaggttgat ttcattaccc
    23221 ggaatgacca tcgcaataga taaaccgcca acagagctta agcagattgc cgccaccatc
    23281 agcagtaaca tagccagtgg atagtcgcgc ccggggttgc tcatttcatt gacgtgagtt
    23341 gcggaagctt ctacgcccat ataactcaaa atgaaggcaa caaatacaac cagtgtgccc
    23401 actttagaga agtcagggaa gaaggtcttc gaatccattt cgatagcaac gggggcaccg
    23461 gagtgcagat aaatagccgc taatgcgatc aaaataaatg caggtaacag gataccggcg
    23521 aagaagccaa ctttagcaat tcgcgccgtg tatttcgtgc caccaaactg cgttaatgcc
    23581 agcgcccaaa gaatgatgag tgctgcaata gttttggtaa tggggtcttc attcagcgct
    23641 ggccatttca ggatgtagga gagtgcccct aacacgaaat agagcatcgg aataaaacca
    23701 atggcgattt gcagatagcc aaatgagatc gctgcaaatc cccatctcgg ccccagagta
    23761 tttgataccc aggcgaagac accaccttct tcccagccgt cgacggtggc catttccgca
    23821 gcacaaagtc ccacgggaat aaaccataaa atcccgccta atagcaggaa gaagactaat
    23881 gaaaagcccg atgttgcgaa ggtagggtat tcataaacag ccattaccat cgatgccgtt
    23941 atggcaaaga atccgagtaa tgtgagctgc ttagctttac ctgtctgtac tgatgtagcc
    24001 atattatccc cctaaaacgg tattcctgtc ggaaccgcac ctgttttgtt ctgaagcgta
    24061 ttcagaacaa tattttccgt tgctaatgcc agtgaacaga ctttggaaat tgtcccgaaa
    24121 cgggttcgtt tcgggacacc gttaccgtta aacgttatca ggt
//
LOCUS       DADLTD010000042        24059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLTD010000042 DADLTD010000000
VERSION     DADLTD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.042"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            322..549
                     /gene="feoA"
                     /locus_tag="JN701_001352"
     CDS             322..549
                     /gene="feoA"
                     /locus_tag="JN701_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY7363856.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            566..2887
                     /gene="feoB"
                     /locus_tag="JN701_001353"
     CDS             566..2887
                     /gene="feoB"
                     /locus_tag="JN701_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY7363857.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2887..3123
                     /gene="feoC"
                     /locus_tag="JN701_001354"
     CDS             2887..3123
                     /gene="feoC"
                     /locus_tag="JN701_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY7363858.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3326..4204
                     /gene="rpnA"
                     /locus_tag="JN701_001355"
     CDS             3326..4204
                     /gene="rpnA"
                     /locus_tag="JN701_001355"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY7363859.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="JN701_001356"
     CDS             complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="JN701_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY7363860.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQSLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5041..5724
                     /gene="gntX"
                     /locus_tag="JN701_001357"
     CDS             5041..5724
                     /gene="gntX"
                     /locus_tag="JN701_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY7363861.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5783..6358
                     /gene="nfuA"
                     /locus_tag="JN701_001358"
     CDS             5783..6358
                     /gene="nfuA"
                     /locus_tag="JN701_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY7363862.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6719..8035
                     /gene="gntT"
                     /locus_tag="JN701_001359"
     CDS             6719..8035
                     /gene="gntT"
                     /locus_tag="JN701_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7363863.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="JN701_001360"
     CDS             complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="JN701_001360"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY7363864.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10174..12567)
                     /gene="malP"
                     /locus_tag="JN701_001361"
     CDS             complement(10174..12567)
                     /gene="malP"
                     /locus_tag="JN701_001361"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY7363865.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13179..15884
                     /gene="malT"
                     /locus_tag="JN701_001362"
     CDS             13179..15884
                     /gene="malT"
                     /locus_tag="JN701_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY7363866.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15927..16943)
                     /gene="rtcA"
                     /locus_tag="JN701_001363"
     CDS             complement(15927..16943)
                     /gene="rtcA"
                     /locus_tag="JN701_001363"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY7363867.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(16947..18173)
                     /locus_tag="JN701_001364"
     CDS             complement(16947..18173)
                     /locus_tag="JN701_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7363868.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18362..19960
                     /locus_tag="JN701_001365"
     CDS             18362..19960
                     /locus_tag="JN701_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY7363869.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19942..20700)
                     /locus_tag="JN701_001366"
     CDS             complement(19942..20700)
                     /locus_tag="JN701_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY7363870.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20717..21547)
                     /gene="glpG"
                     /locus_tag="JN701_001367"
     CDS             complement(20717..21547)
                     /gene="glpG"
                     /locus_tag="JN701_001367"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY7363871.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21592..21918)
                     /gene="glpE"
                     /locus_tag="JN701_001368"
     CDS             complement(21592..21918)
                     /gene="glpE"
                     /locus_tag="JN701_001368"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY7363872.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22108..23613
                     /gene="glpD"
                     /locus_tag="JN701_001369"
     CDS             22108..23613
                     /gene="glpD"
                     /locus_tag="JN701_001369"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY7363873.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23665..>24059)
                     /locus_tag="JN701_001370"
     CDS             complement(23665..>24059)
                     /locus_tag="JN701_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363874.1"
                     /translation="VMAGIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYIS
                     PEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKNR
                     NQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5653 a         6553 c         6082 g         5771 t
ORIGIN      
        1 caaactcata atatattccg gcaatattta tcacttcatt aacaactgaa accttaatta
       61 aacattagcc agtccgggta attcactatt cgaattatat tttcgctgcg atataacctt
      121 gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca cattttaagt
      181 attgctgata gaaaccattc tcattatcat tgtgttattg attatttaat ctctcctttg
      241 ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt tatccagtta
      301 atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa aatcactggc
      361 ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctcttgg catgttacct
      421 ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc
      481 cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt
      541 tcctgttaat acggtgataa caacaatgaa aaaattaacc ataggcttaa ttggtaatcc
      601 aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc gtgtaggtaa
      661 ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt
      721 cacgctggtg gacctacccg gcacctattc tctgaccacc atctcatcgc agacctcgct
      781 cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc tgattaacgt
      841 ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctacaactgc tggaactcgg
      901 cattccctgc attgtggcac tgaacatgct cgacattgcc gagaagcaaa atattcgtat
      961 tgaaattgat gctctgtcgg cgcgtctggg ctgtccggtg atcccgctgg tttcaacccg
     1021 tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt
     1081 ggaactggtg cattacgcac agccgctgct caacgaagca gattcactgg caaaagtgat
     1141 gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga
     1201 tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg ccctcgcccg
     1261 tctgcgtaat gagatggacg atccggcgct gcacattgcc gatgcgcgtt accagtgcat
     1321 tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac
     1381 tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat
     1441 gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt
     1501 cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc tccacttccc
     1561 ggactggctg actatcttcc tcgcccaggg cctgggtggc ggcattaaca ccgtgctgcc
     1621 actggtgccg cagattggca tgatgtacct gttcctctcc ttccttgagg actccgggta
     1681 tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag gcgctgggct tgccggggaa
     1741 atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa tgggtgcacg
     1801 tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg
     1861 cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc
     1921 gctggcggtc ttctcgctgt atatgctggg tattgtgatg gcggtgctga ctggcctgat
     1981 gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagt tgccggtcta
     2041 tcatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga aaggcttcgt
     2101 tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc ctgagcgctt tcaacagctt
     2161 ctcgctgagc gggaaaatcg tcgataacat caacgactcg gcgctggcgt ccgtcagccg
     2221 ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa gataactggc aggcaacggt
     2281 tggcctgttt acaggtgcca tggcgaaaga agtggtagtg ggtacgctca acaccctcta
     2341 caccgcagaa aatattcagg acgaagagtt caatccggca gagtttaacc tcggtgaaga
     2401 gctgttcagt gcgatagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt
     2461 actgatgaac cccattgaag ccagcaaagg caacggcgaa atgggtaccg gggcgatggg
     2521 cgtgatggat cagaaattcg gtagcgcagc agcagcttac agctacctga ttttcgtcct
     2581 gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg
     2641 gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa cattgttcta
     2701 tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc tggcggttat
     2761 cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg tggatatcga
     2821 actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca ccggtgattg
     2881 ccattaatgg cttcacttat tcaggtgcgc gatttgctgg cgttacgggg ccgtatggaa
     2941 gcggcccaga taagccagac attgaacact ccacagccaa tgattaacgc catgctgcaa
     3001 caactggaaa gtatgggcaa agccgtgcgg attcaggaag aacctgacgg ctgcctctct
     3061 ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc gcgagtggtg ggcgcttcgt
     3121 taaccttact ccatcgcctg ttttggaaag cccggtatgc gtctgcatcc gggctttttt
     3181 gcgtgcggct ttccataaaa atgcaactct tgcagcacgg cgtaagttcc tttgaaagca
     3241 tctcgcaggg atgaaaactc gctaatacac aggtgtggag tggcgcgtag agtcgcggca
     3301 ttcaaacaac aggtgaagga acgccatgag caaaaagcag agttccaccc cacacgatgc
     3361 gctgttcaaa ctctttttac gccaaccgga cacggctcgt gattttcttg cgtttcattt
     3421 accggcaccc attcacgcgc tttgtgatat gaaaaccctc aagctggagt cgagcagctt
     3481 tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga aaacggaaca
     3541 aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca aactgatcgc
     3601 atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg ctggatacaa
     3661 aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc cctatcccta
     3721 ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc tttatgcctc
     3781 cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg
     3841 tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgacctga tggggctagt
     3901 agagcaaatg ggctgcttat taagtagtgg atacgctaat gacagacaaa tcaaagggct
     3961 gtttaattac atactgcaaa ccggcgacgc ggtacgtttt aacgatttta tcgacggcgt
     4021 tgccgaacgt tcaccgaaac acaaggagag tttaatgaca attgcggaaa gattgcggca
     4081 ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat ccggagtccc
     4141 tcttacagac atcatgcgct ttaccggggt gtcagaagaa gagttggctg cggcgagcca
     4201 gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc tctgcttcaa cgccaccagc
     4261 aggtgacaaa actcgaccgg atgcgaaata aacggcgcat gggccgcttt ggcgaagata
     4321 tatgatttgc tgcgaggcca gagtttatcc agcatcggca ccactttgcg cggcaccaga
     4381 ccatcgagat agccatacaa tcgcaaaaac ggcatgggca cgttttgcag cgactgacgg
     4441 agatcaaccg ttttcaggat ttccagcccg ccattaagca cgtcaacctc cggcatcggt
     4501 aacgccagaa cggttttctt caacgcccgc gcatcctggc gcgccgtttc agtccccatg
     4561 gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat catcactgag ttgctgctga
     4621 aatcccgcca gcacgtccgg ttttatcccc ggccaatcgt cacgagcact aaaacaaggt
     4681 gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc aatctggctt
     4741 gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc ctgttgcagc
     4801 acggcttcgg ccatatcagc aagtgacagc gcaccaaatc cctggctgcg cccgaagccg
     4861 ggcagatcaa caaggtgcag cgtaaaatgc gagctaagtt cctcgtcaat gcaacgccac
     4921 acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc ctgacctttg
     4981 gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa ggatgaacat
     5041 atgctaacag taccgggatt atgctggcta tgccgaatgc cgctggcgtt aggtcattgg
     5101 gggatttgtt cggtctgctc acgcgccgcc cgtacagata aaacgttatg cccacaatgt
     5161 ggattacccg ccacacactc acatcttccc tgcggtcgct gcctgcaaaa accaccgccc
     5221 tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct tatccaccag
     5281 cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt cacgtctgtt actattggaa
     5341 gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcatcat cagcgttccg
     5401 ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga gcgatttgct gtgtcagccg
     5461 ttatcacgct ggttgcactg ccgatgggat agcgaagccg tcacacgaac acgggccact
     5521 gcgacccagc attttctcag tgcccggcta cgcaagcgca acctgaaaaa tgcctttcgt
     5581 cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt taccaccgga
     5641 agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac tgtccaggtc
     5701 tggtgccttt gtcgaacctt gtagagcctc gatgataggc gtattataac caactaaaat
     5761 agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag cgcactttgc
     5821 caaactgctg gcaaaccagg aagaagggac acaaatccgc gtatttgtga ttaaccctgg
     5881 cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg gatgccgtgg aagccaccga
     5941 cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg ctccttacct
     6001 ggaagatgca gagatcgatt ttgttaccga ccagttgggt tctcagctaa cactgaaagc
     6061 gcctaacgcc aaaatgcgta aagtggcaga cgatgcacca ctgatggagc gcgtggagta
     6121 tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggccgcg tttcactgat
     6181 ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta acggttgttc
     6241 catggtcgat gtgacgctga aagaagggat cgagaagcag ctgctaaacg aattcccgga
     6301 gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gaacactcct actactaagt
     6361 tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg gcctacgttg
     6421 aacatgtgcc agacgttggc gcagcattgc tttggcaaat ccctcccttc gtttctccgc
     6481 gataatatga ccaacctctc ataatttaaa tttactccgc tctggtgatt ctcaaacgcc
     6541 agatgttacc ggtatcattc acatgggtac caaacatact cctgacatct gactacaata
     6601 attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga ttgggataat
     6661 tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa tgaaaggcca ggtaaatcat
     6721 gccattagtc attgttgcta tcggtgtaat cttgttgttg ctcctgatga tccgtttcaa
     6781 aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct gttggattaa tgcaagggat
     6841 gccgctggat aaagttattg gctccatcaa agccggtgtc ggcgggacgc tcggtagcct
     6901 tgccctgatc atgggttttg gcgcgatgct gggcaaaatg ctggcagact gcggtggcgc
     6961 acaacgtatc gccaccaccc tgattgccaa atttggtaaa aaacacatcc agtgggcggt
     7021 ggtactgacc ggttttaccg ttggttttgc cctgttctat gaagtgggct ttgtgctgat
     7081 gctgccgctg gtgtttacca tcgcggcttc tgcgaatatt ccactgctgt atgttggtgt
     7141 accaatggca gctgcactgt ctgtgaccca cggcttcctg ccaccgcatc cgggtccgac
     7201 tgcgattgcc accattttca atgccgacat gggtaaaacc ctgctgtacg gtactattct
     7261 ggcaatcccg accgtgattc tcgccggtcc ggtttacgct cgcgtgttga aaggtatcga
     7321 taagccgatc cctgaaggtc tgtacagcgc gaaaaccttc agcgaagaag agatgccgag
     7381 ctttggcgtc agcgtctgga cctctctggt gccggtagtg ctgatggcga tgcgtgcgat
     7441 tgccgaaatg atcctgccga aaggtcacgc tttcctgccg gtagcggagt tcctcggtga
     7501 cccggtaatg gctacgctga ttgccgtgct gattgcgatg ttcacctttg gtctgaaccg
     7561 tggtcgttca atggatcaga ttaacgacac gctggtttct tccatcaaaa tcattgcgat
     7621 gatgctgttg atcatcggtg gtggcggtgc gttcaagcag gtgctggtag acagcggcgt
     7681 ggacaaatac attgcttcca tgatgcacga aaccaacatt tctccgctgc tgatggcctg
     7741 gtcgattgct gccgtactgc gtatcgcgct gggttccgca accgttgcgg caatcactgc
     7801 gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt agcccggagc tgatggttat
     7861 tgcggtgggt tccggtagtg tgattttctc tcatgtgaac gatccgggct tctggctgtt
     7921 caaagagtac tttaacctga ctatcggcga gaccatcaaa tcctggtcga tgctggaaac
     7981 cattatctcg gtgtgcggtc tggtaggctg tctgctgctg aatatggtga tttgataaca
     8041 ctgactgccg gatgcatcgc atccggcatc aaccgcactc tactgcttct tcgctgcagc
     8101 tctgcgccgt ctgtccaaat ccttcagcaa cttattcacg ccatcatcgg caaacatcgt
     8161 ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac tggtaactgg tgccaggaat
     8221 attcaccggt tcggccatat ccagccagtc ttccggctgt agtcctaaca gagcactgtt
     8281 actgtcggca atgtagcgct gcaaaccacg attaagcgtc ggcgtcatcg acatcaacga
     8341 tgccttatgc ccggcacgtt ttggcagaca accatattta tgcagtgcat ccagcagccc
     8401 ttgcttcgcc agttcgcgat cctgatacag accgcgcagt accacttcat ccggatacag
     8461 ccccagggtt ttgcccaacg ttagatcccc gctctcccag taaccgcgca gcgtaggcag
     8521 gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac gctttcggtg cacggaacgt
     8581 cttctcgtgg tcgttttcga aatagagcac tttgtaggag tacacaccgc tgctgcgcag
     8641 cttaccgaca atctctaccg gtacggtacc gagatcttca ccaatcacca tacagcgatg
     8701 acgtttactt tcgagtgcca gaatcgagag cagatcatcc accggatagt gaacatacgc
     8761 gccctgatct gccgtctcgc catacggtat ccaccacaaa cgcagcatcg acatcacatg
     8821 gtcaattcgt aatgcgccgc agttttgcat attggcacgc aacagctcga taaacggttc
     8881 ataggcacgc gcggtgatga tatgcgggtc cattggcggt aatccccagt tctgccccaa
     8941 cgggccgagg atatccggcg gcgcgccaac cgacgctttc aggcaatata gttcacggtc
     9001 acaccaggtt tccgccccac cttccgctac gccaaccgcc agatcacgat acaagccaat
     9061 cggcatttca tagccctggc ttatctccca gcaggcggca aactggctgt aagccagcca
     9121 ctgcaaccag agataaaaat cgacgtcatc acgatgttct tcgcagaact gacgcacttc
     9181 tggtgaatcc acgttctgat acatctctgg ccatgcaggc cagccccagc gcatttcgtc
     9241 ctctttcact tgctgggcat gtagcgcatc aaaggctgcc tgccagaaca ggctgtcgcc
     9301 ctgctctgca acaaactggc gaaacgcggt catctgctca tcatcacgtt gcgcgaaacc
     9361 tttccacgcc attcgtaatg ctgtcatttt tagggcggta accgtggagt aatcgaccca
     9421 gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc ggcaactgcc accaggcctg
     9481 agcctcttcg ctaagatgga aatcttcaac ggcgttaacg tcgatataaa tcacattcag
     9541 ccaacggcga gaagacggac tgtatgggct ggcactctcc ggatttgccg gatagagcgc
     9601 atgaatcggg ttcaggccga tgaacgaccc gccacgtttt gccacatcca ccagcatcgc
     9661 tttgagatcg ccaaaatccc caatacccca gtttttttcc gatcgcagcg tataaagctg
     9721 aacgcaggca ccccacagct tttgtttatt cagcaacgcc tgcggttcgt aacagcgttt
     9781 cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc tgggtgagtg tcagcgtgtg
     9841 ataaccttcc ggcagcttcg tcggtagatt gaacgctttg ccccccgtta catggccttt
     9901 gtactgtgtt ccttcttcgg tggtcagcag ccagctatat tcgccgctgc cctccaccac
     9961 catcggcatt tttttgccgc tggtataaac catgacattc gggactggcg ttaccgccac
    10021 tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc cgtttggttt cggcgctaat
    10081 cgactgcggt ttaccgtggg cattgatgta attggggcta atccccgccg ccagcgcggc
    10141 attatccaga cgtttgcttt ccatcgagct tccttagcgt tttgcctgcc agatacgagc
    10201 ctgataatcg cgaatagagc gatccgagct aaacataccg cagcgggcgg tattgaggat
    10261 cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca tccacctgct tttgtgcctc
    10321 aacataggct gcgaaatccg ccatcaccag atacggatcg ccgccttgtt tgccgatact
    10381 gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg tatttaccgc tttccagctc
    10441 tttcaatact gcgtccagca ccttatcttt cttccgccat ttcaccgggt cgtagccttt
    10501 ggccagaatt gccttcactt gttccacggt atgaccaaaa ataaagatat tttcttcacc
    10561 gactttctcg gcgatttcaa cgttcgcacc atccagcgta ccgacagtaa gcgcaccatt
    10621 gagcgccagt ttcatattgc cggtaccgga agcttcttta cctgcagtcg aaatttgttc
    10681 ggagatatcc gccgccggga tcagtttttc cgccgccgaa acgcaataat cctgcaggaa
    10741 caccaccttc aacttatcgc caaccagtgg atcgttgttg atcacgtcag ccactttgtt
    10801 gatcgcaaag ataatattct tcgccaggta gtagcccggt gccgctttcg cgccgaagag
    10861 gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga atttctttgt acaacgccag
    10921 aatatgcagc agattcaggt gctggcgttt gtactcatgc agacgtttaa tctgaatatc
    10981 gaaaatcgcc tgtggattga tctcaatacc ggtacgaact ttcacaaact ccgccagacg
    11041 gactttattc gcctgcttga tctcgcgata ttgctgacgg aatttcgcat catcagcgaa
    11101 tttttccaga ttgatcagct gatcgagatc gttagcccac tctttttgca gtaatttatc
    11161 caacagagcc gccagtgcag ggttgcactg tttgatccag cgacgtgggg taataccgtt
    11221 ggtgacgtta tggaatttgt tcggccatag ctggtgatat tccgggaaca gatctttcac
    11281 caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg aaaccgccaa ccacacacag
    11341 gttcgccata tgcacttgtt tatcgtgcac caccgccagt ttggcccaca ctttttcatc
    11401 gcccggccag gttttctcca ccagcgtttt aaagcgagta ttaatttcgt taataatctg
    11461 catatggcgc ggcagtaagc ctttcaccag tttcacatcc cagcgttcca gcgcttctgg
    11521 catcagggta tggttggtgt aagcgaaagt tttgctggta atggcccagg cgtcatccca
    11581 gctcatctgg tgctcatcga tcagcacgcg cagcagttct ggaatcgcga tagttgggtg
    11641 ggtatcgttc agctgaataa cttcataatc cgccagttcg tgcagtttac gccccgccag
    11701 atgatggcga cgcaaaatat ccgctaccga acaggcacac tggaagtact gctgcatcaa
    11761 gcgcagcttt ttaccggcag tatggttgtc gtttggatag agaactttgg tcagtttttc
    11821 cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg tcgttaaatt tagtcagatc
    11881 aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc tgcgccacgc cattacgata
    11941 gccgacaacg gggagatccc acgcttgacc ggtaatggta aactccggct cccagcgtcc
    12001 gtctttcgtc actttaccgc caatccctac ctgcacatcc agtgcttcgt tgtggcggaa
    12061 ccacgggtag ttactgcgat gccagtcatc cggcgcttca acctgtttgc catcgacaaa
    12121 agactggcgg aacaaaccat attgatagtt cagaccgtaa cccgtcgcag actgaccgat
    12181 agttgccatt gagtcgagga agcacgccgc cagacgtccc agaccaccgt tacccagcgc
    12241 cgggtcgatc tcttcttcca gcaggtccgt cagattgatg tcataagcct tcaacgaatc
    12301 ctgtacttcc tgataccagc cgagattcaa caggttgttg cccgtcaggc gaccaatcaa
    12361 aaactccatt gagatgtagt taacatgtcg ctgattcgcc accggcttgg cgaatggctg
    12421 agcacgcagc atttcggcca gtgcttcact cactgctagc caccactggc gaggagtcat
    12481 ttcagccgca gaatttaagc cataacgctg ccactgacgt gaaagcgctt cctgaaattg
    12541 cttatcgtta aaaataggtt gtgacatagg agttccactt ttcttagatt ttcaacacaa
    12601 cgttatcgct agtttgccag gctcgatgtt gaccttcctc atcctgcggg ggattaggca
    12661 gggaggagtt gcggggatga gcaaggaaat gtgatctcaa ccacttaaag ctagtgcaaa
    12721 ccacaggatt agcagcaaat caatgcaata cagcgcagaa aatctgtatc taagtgcaaa
    12781 aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc tataaattaa gtaaaggagt
    12841 gaaacagttt cacaagtaaa atatccagtg tgctccatct cattcttaat agatttatta
    12901 agatcatctt tttagatggc actttcatca ggaatgaaga agaaaccctt gcttaaatga
    12961 atctgatgaa cataagggaa accagtattc acgctggatc agcgtcgttt taggtgagtt
    13021 gttaataaag atttggaatt gtgacacagt gcaaattcag acacataaaa aaacgtcatc
    13081 gcttgcatta gaaaggtttc tggccgacct tataaccatt aattacgaag cgcaaaaaaa
    13141 ataatatttc ctcattttcc acagtgaagt gattaactat gctgattccg tcaaaactaa
    13201 gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg cctgctggct aaactttccg
    13261 gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc gggctacgga aagaccaccc
    13321 tcatttccca gtgggcggca ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg
    13381 ataaccagca agagcgtttc gccagctatc tcattgccac cgtgcagcag gcaaccaacg
    13441 gtcactgtgc gatatgtgag acgatggcgc aaaaacggca atatgccagc ctgacgtcac
    13501 tcttcgccca gcttttcatt gagctggcgg aatggcatag cccactttat ctggtcatcg
    13561 atgactatca tctgatcact aatccagtga tccacgagtc aatgcgcttc tttattcgcc
    13621 atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa ccttccgcaa ctgggcattg
    13681 ccaatctgcg tgttcgtgac caactgctgg aaattggcag tcagcaactg gcatttaccc
    13741 atcaggaagc gaagcagttt tttgattgcc gtctgtcatc gccgattgaa gccgcagaaa
    13801 gcagtcggat ttgcgatgac gtttccggtt gggcgacggc actacagcta atcgccctct
    13861 ccgcccggca gaatacccac tcagcccata agtcggcacg ccgcctggcg ggaatcaatg
    13921 ccagccatct ttcggattat ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc
    13981 gccattttct gttgaaaagc gccattttgc gctcaatgaa cgatgcgctc atcacccgtg
    14041 tgaccggcga agaaaacggg caaatgcgtc tcgaagagat tgagcgtcag gggctgtttt
    14101 tacagcggat ggatgatacc ggcgagtggt tctgctatca cccgctgttt ggcaacttcc
    14161 tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg
    14221 cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag
    14281 gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata
    14341 gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa
    14401 atccgcagtt agtgttattg caggcgtggc taatgcaaag ccaacatcgc tatggcgaag
    14461 ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc
    14521 acgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat ccggatgaag
    14581 cggaacggct ggcaaaactg gcactggaag agctgccacc gggctggttc tatagccgca
    14641 ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac
    14701 tggcgctaat gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct
    14761 tatggagttt aatccagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt
    14821 gggaaacaca ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccga
    14881 tgcatgagtt tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg
    14941 aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac
    15001 agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg
    15061 cccgtagcca gctgaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact
    15121 ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg
    15181 ccgctgccaa ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc
    15241 aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg
    15301 aaattgttct cgaagaactc aatgaaaatg cccgcagtct gcggttgatg agcgatctca
    15361 accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc
    15421 agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg
    15481 tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct gattcaactc aatacgctgc
    15541 cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc
    15601 ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc
    15661 cagaactgat ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct
    15721 actccggtta cagcaatgag caaattgcgg gcgagctgga agtggcggca accaccatca
    15781 agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc ccaccgtcag gccgcggtac
    15841 aacacgcgca gaaactgctg aagatgatgg gatatggggt gtgagtttag ccggataatg
    15901 cattggatcc ggtttacatc tttacatcat tcaatactca cccgcgttac gccatctgtt
    15961 tctatcaaac caaaccgcac cggcaagaaa cgctccacca cggcgatatt ggtcagcaga
    16021 tggcatgaag gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggtagcacc
    16081 agctggtcgg cgagatattc ccccactgcc gccgggcttg ccaggtagcg tttcacctct
    16141 ttcactaact gcgcggctac cacctcggca ctgacgcgct tttcaccgac gacgaaaaag
    16201 cgttcagtga tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg
    16261 cgcggcaggt tatgaatatt ctgttcatgc agggaaaaac tccccgccag tgtagcgatt
    16321 tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg catctgcaca
    16381 atgttcccgc gctcgccaag ttgcaaggtg ttaaacgatg ccacgggcga gacttccgtt
    16441 gccaccacgc cgcctccggc aggataaaaa ccgtgacgta acagcgtggt ttgctgatga
    16501 attcctattt tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggcgcc
    16561 gacgggttat cggtgcctcc gctcacttca acacgcgaag gtccatcggc aaaccacagc
    16621 gcgggcagca ccgtttgcag caccagcgta cagcttccgg cactaccgat agcaaagcgg
    16681 tactcgccgc cgcgcacggc gccaggccgg aagaccagac gctgcgaccc cagctccgcg
    16741 ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc
    16801 aacagccccg gtttcgcccg cccggcacga atgctggtga tggtaaatgg ctggccggtt
    16861 atcatcgaca ggctcagcgc cgagcgcaga atctgcccgc caccttcgcc ctgtgcgcca
    16921 tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggta
    16981 tagataactt ccaccagatc gctttgtgcc gtcatcaccg catcaatatc tttatacgcc
    17041 atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga
    17101 atttgatctt ccacgctgaa cagttttttc gctttagttc ggctcattac ccgcccggca
    17161 ccgtggctgc acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttt
    17221 gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac cgcgccttta
    17281 cgcgtcacgt agatctcttc accaaagtgc tgttcttttt gcacatagtt gtggtgacag
    17341 ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc
    17401 aatgccgtta ccacgttttc catcatcaca tcgcggttaa ggctggcaaa aagctgcgcc
    17461 caggccacgg ctttcaggta atcatcaaag tattccgtac cttccataaa gtacgccaga
    17521 tcacgcgacg gcaacgtctc aagcgtttcc tgcatctctt tttgtgccag atcgataaag
    17581 taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgg
    17641 tccgactcat caaggcagat tttaataaag tggttaccgg ttcccagcgt tcccaggtgt
    17701 ttatagttat tggtattcag gaaacgggga tatttttgcg ttaaccactg ataaccggct
    17761 tcaagctcag cccatttcgc atcgacgttg acaggcgggt tttcccatgc gcctttatca
    17821 cgtttacaac gcccggtagt acgcccgtgc ggcacggccg tttcaatcgc ctgacgcagc
    17881 tctgccaggt tttcaggcag gtcttccgcc gttaacgcgg tacgcagcgc gttcattcca
    17941 cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta
    18001 ccaatggtgg aatctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata
    18061 aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac cggcacgcct
    18121 ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt
    18181 ttctcttttc gttgtttgct gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa
    18241 gtaaattaat tttttaattc attgttttta aaaagattat atctttatgt ccgtaaccgg
    18301 agatttcccg caaagccgat ttaccgataa tgaaatatcg tcttttataa ggatatctaa
    18361 gatgcgtaaa acagtagctt ttggctttgt cggaacggtg ctggattatg ccgggcgcgg
    18421 cagtcagcgc tggtcaaaat ggcatccgac actctgttta tgccagcaag aatcgttggt
    18481 catcgatcga ctggaattat tgcatgacgc ccgctcgtgc tcgctatttg aaacgcttaa
    18541 acgcgatatc gccagcgttt cgccagaaac agaagtggtg agcattgaga ttgaactgca
    18601 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacaa
    18661 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
    18721 gatttgctgg tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaaacttc
    18781 accaccgcgc aaaaaagaac aaccgcgcgg tgccggtgaa gtgacgatta tcgatctcga
    18841 tctaagccgt tataacgcca tcgccagccg ctttgccgag gaacgccagc aaacgcttga
    18901 ttttcttaag tccggcattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
    18961 tgaaaaagtg gcgattaaat cccgcgcacc gatcctgctt aacggcccaa ccggtgcggg
    19021 aaagtcattt ctggcgcgac gtatatttga attaaaacag gcgcggcatc agtttagcgg
    19081 cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgccatgt cgacgctgtt
    19141 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggtt tattacgcag
    19201 cgccaacggc ggaatgttgt tttttgatga gattggcgaa ctgggcgcag acgaacaggc
    19261 aatgctgcta aaagccattg aagagaaaac cttttacccg tttggcagcg atcgccaggt
    19321 gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga
    19381 aggcaaattt cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
    19441 tctacgccag cgccaggaag atattgaacc gaaccttgat tatgaagtgg agcgccacgc
    19501 cacactcact ggcgacagcg tgcgttttaa caccgaagcg cggcgcgcct ggttggcttt
    19561 tgcgacctct ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac
    19621 gcggatggcc acctttgcca ctagcggacg catcactctg gacgtggttg aagatgagat
    19681 aaaccgtctg cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg
    19741 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
    19801 ctgccgccag gcaaagtcgc tttctgcagc cggacgacag ctttttgatg tttcgcgcca
    19861 gggaaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
    19921 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatgatggt ccgtcagcac
    19981 ctgcattacg cttactggcg gcggggcgtc ggtgtagacg gcatctacca tgctgatgct
    20041 gcccatattg accatcgcgt tacggccaaa tttcgagtga tcgacaacca gcataacgtg
    20101 gcgcgagttc tcaataatgg cgcgcttggt gcgaacttcg tggtaatcga actccagcag
    20161 cgagccgtcg ctatcgatgc cgcttatccc cagaatgccg aaatcaaggc ggaactggga
    20221 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctgcgta attcgccacc
    20281 agcgagaata atgcggaaat cttcttttac catcaacgtg ttagcaacat tgagattgtt
    20341 ggtgacaata cgcaaattgc tgtgattaag cagcgcgtgc gccaccgctt ccggcgtggt
    20401 gccgatatcg ataaacagtg tcgagccgtt ggggatttgc tccgccactt tgcgggcaat
    20461 cctctctttt tcttcggtct gggtggcttt gcgatcgtgc cacggcgtgt taaccgaact
    20521 ggaaggcagc gccgcaccgc catggtgacg caggatcagg ttttgctccg ccagctcatt
    20581 gaggtcgcgg cgaatagtct gcgggctgac ggagaaatgc tctaccagct cttcagtact
    20641 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
    20701 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
    20761 ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
    20821 caaaccatcc ggcgataatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
    20881 tgccactttg cggatcgcgc tcaccacgta gccagacgta gcccatcagc gcatacacca
    20941 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcacatag ccgcttaaca
    21001 gggcactaat gagagtaata acaattagct taccgctacc gaggcgtttt tccaccgcac
    21061 cgccgagata ccaccaccag agcaggttaa agaggatatg catcagcgag aagtgcatta
    21121 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca
    21181 gccataacat cacttcctga tcgccgagaa tttgcatggc gataaacacc accacgcagg
    21241 cgatcatcac cacccaggtt accggacctg cgcgttcacg caaggcagca aagaaaggat
    21301 aacggcgata atgcagacca ctgccggtat ggcccgcctg ccagctggcc gccagataac
    21361 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg
    21421 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtaagg ataacaccct
    21481 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
    21541 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
    21601 gtacgccacc tctgcgggaa actgacgctg ccaggcttca aaaccgccgt caatgctata
    21661 gaccacatcg tagccctgtt gcagcagata ctgcgccgcg cctttgctgc tattgccgtg
    21721 ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt
    21781 gtcgttggtt aaatggaaag cctgcaccgc atgtcccatt gcgaaactct gtggatcgcg
    21841 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
    21901 acattcgaac agatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
    21961 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
    22021 ttttcaatgt tacctaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
    22081 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
    22141 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
    22201 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
    22261 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
    22321 gaagtgctgc tgaaaatggc tccgcatatc gccttcccaa tgcgttttcg cctgccacat
    22381 cgtccgcatc tgcgcccggc gtggatgatt cggattggtc tgtttatgta cgatcatctg
    22441 ggtaaacgca ccagcctgcc gggatcaacc ggtttgcgtt ttggcgcaaa ctcagtgtta
    22501 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
    22561 gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc
    22621 gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
    22681 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
    22741 cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
    22801 agccatattg tggtgccgcg tgtgcatacc cagaagcagg cctacattct gcaaaacgaa
    22861 gataaacgta ttgtgttcgt gatcccgtgg atggacgaat tttccatcat cggcaccacc
    22921 gacgtagagt ataaaggcga tccgaaagcg gtgaagattg aagagagtga aatcaactat
    22981 ctgctgaaag tgtataacgc gcactttaaa aaacagctta gccgtgacga tatcgtctgg
    23041 acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca ggctattacc
    23101 cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc
    23161 ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg
    23221 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
    23281 gaaggcgacc gcgacgatta cgcagctcgt ctgcgccgtc gctatccatt cctgactgaa
    23341 tcgctggcgc gtcattacgc acgcacttac ggcagcaaca gcgagctgct gctcggcaat
    23401 gcgggggcga taagcgacct cggggaggat ttcggtcatg agttctacga agcggaactg
    23461 aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctttg gcgtcgcaca
    23521 aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa
    23581 tatacgcagc agaagttgtc gctggcgtcg taaggatgat gcccggttga agtgcaaccg
    23641 ggctatttgg attccttaga ctattcattg taaataattg aataacggcc cctgagctaa
    23701 aatcgccaaa cttaagcacg ttacccaatc gccccacttc ttcctctggt ttctattttt
    23761 tctgcaccaa tggcaataat atcccaatgc aaaaccaagc tgtgcactaa tcgggccgac
    23821 aaaatcagtc caaggctgcg gccaaaaagg ggaaaaattc aggggaggcc agacaaacgt
    23881 cagaaagaaa tttcggatat tttccagcaa cgctgatttc tctggagaaa tataaagaat
    23941 gaaattcgag agaattaacc agaaatagac cggcgaaaag accagccagc aatgcagtct
    24001 gttttttaat tcaggaggcg ttctgcggga gaattgccaa ccgatgccag ccattacac
//
LOCUS       DADLTD010000043        24031 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLTD010000043 DADLTD010000000
VERSION     DADLTD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.043"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(214..1500)
                     /gene="thrC"
                     /locus_tag="JN701_001371"
     CDS             complement(214..1500)
                     /gene="thrC"
                     /locus_tag="JN701_001371"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY7363875.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1501..2433)
                     /gene="thrB"
                     /locus_tag="JN701_001372"
     CDS             complement(1501..2433)
                     /gene="thrB"
                     /locus_tag="JN701_001372"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY7363876.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2435..4897)
                     /gene="thrA"
                     /locus_tag="JN701_001373"
     CDS             complement(2435..4897)
                     /gene="thrA"
                     /locus_tag="JN701_001373"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY7363877.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(4977..5042)
                     /gene="thrL"
                     /locus_tag="JN701_001374"
     CDS             complement(4977..5042)
                     /gene="thrL"
                     /locus_tag="JN701_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY7363878.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5256..5942)
                     /locus_tag="JN701_001375"
     CDS             complement(5256..5942)
                     /locus_tag="JN701_001375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7363879.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6342..6482)
                     /locus_tag="JN701_001376"
     CDS             complement(6342..6482)
                     /locus_tag="JN701_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363880.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6578..7294
                     /gene="arcA"
                     /locus_tag="JN701_001377"
     CDS             6578..7294
                     /gene="arcA"
                     /locus_tag="JN701_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY7363881.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7354..8706)
                     /gene="creD"
                     /locus_tag="JN701_001378"
     CDS             complement(7354..8706)
                     /gene="creD"
                     /locus_tag="JN701_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY7363882.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(8764..10188)
                     /gene="creC"
                     /locus_tag="JN701_001379"
     CDS             complement(8764..10188)
                     /gene="creC"
                     /locus_tag="JN701_001379"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY7363883.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10188..10877)
                     /gene="creB"
                     /locus_tag="JN701_001380"
     CDS             complement(10188..10877)
                     /gene="creB"
                     /locus_tag="JN701_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY7363884.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(10890..11363)
                     /gene="creA"
                     /locus_tag="JN701_001381"
     CDS             complement(10890..11363)
                     /gene="creA"
                     /locus_tag="JN701_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY7363885.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11574..12443
                     /gene="robA"
                     /locus_tag="JN701_001382"
     CDS             11574..12443
                     /gene="robA"
                     /locus_tag="JN701_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY7363886.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12440..13087)
                     /gene="gpmB"
                     /locus_tag="JN701_001383"
     CDS             complement(12440..13087)
                     /gene="gpmB"
                     /locus_tag="JN701_001383"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY7363887.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13139..13660
                     /gene="yjjX"
                     /locus_tag="JN701_001384"
     CDS             13139..13660
                     /gene="yjjX"
                     /locus_tag="JN701_001384"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY7363888.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(13745..14071)
                     /gene="trpR"
                     /locus_tag="JN701_001385"
     CDS             complement(13745..14071)
                     /gene="trpR"
                     /locus_tag="JN701_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY7363889.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14161..16098)
                     /gene="sltY"
                     /locus_tag="JN701_001386"
     CDS             complement(14161..16098)
                     /gene="sltY"
                     /locus_tag="JN701_001386"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY7363890.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16309..17976
                     /gene="ettA"
                     /locus_tag="JN701_001387"
     CDS             16309..17976
                     /gene="ettA"
                     /locus_tag="JN701_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY7363891.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18283..19515)
                     /locus_tag="JN701_001388"
     CDS             complement(18283..19515)
                     /locus_tag="JN701_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide
                     kinase/transcriptional regulator NadR"
                     /protein_id="HAY7363892.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19536..20918)
                     /gene="radA"
                     /locus_tag="JN701_001389"
     CDS             complement(19536..20918)
                     /gene="radA"
                     /locus_tag="JN701_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY7363893.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(20967..21935)
                     /gene="serB"
                     /locus_tag="JN701_001390"
     CDS             complement(20967..21935)
                     /gene="serB"
                     /locus_tag="JN701_001390"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY7363894.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22041..22685
                     /locus_tag="JN701_001391"
     CDS             22041..22685
                     /locus_tag="JN701_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY7363895.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            22713..23729
                     /gene="lplA"
                     /locus_tag="JN701_001392"
     CDS             22713..23729
                     /gene="lplA"
                     /locus_tag="JN701_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HAY7363896.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
BASE COUNT         5782 a         6659 c         5956 g         5634 t
ORIGIN      
        1 aggttactcc ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagaa
       61 cgacggttgt tattctccgc aatcctaatc tctaagttac cgcatttatt gagaatatct
      121 cctttttccc tgtcgttttt ctccataatt tcttcataaa aaagccgggc tgcataaaag
      181 caaacccggc ctgattgaga taatgaatag attttactga tgattcatca tcaatttacg
      241 caacgcagca aaatcggcgg gcagattatg tgaaagcaag ggtaaatcag cacgttctgc
      301 cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt
      361 cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggattcaact ggtcacgcag
      421 cgcacgatac gctacggcag cgtgcggctc cgaggtgtag cccagttctt ttaactcacg
      481 cattgtctgt tgcgtggttt catcatccac ggctgcataa cccagctctt tcagttgcca
      541 gattttgcgg cggaacaact cttccacacg cggccagttg ttcggctgac tcacgtccat
      601 cgcgttggat aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg
      661 tggcacggta tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca gaccgagtga
      721 cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agacaaccag
      781 ctggttgcgc gcttcctgcg gcagctgcgc aactgcttca aagtagtagc aaatctgcgc
      841 cagcaaacgg ctgatgttaa tcgagttagc tgagtttaac cccagcgcca ctttcagctc
      901 ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc
      961 aacagtttcg atattgccgc ccaatgtaca gaacagtttt tcttgcagtg gactgatttt
     1021 gcctcgtgga tagaggataa ccactttcac attcggtaaa ccgtagaaag catgagccac
     1081 tgccgctccg gtatcaccgg aggtcgcggt cagaatggtc actggcttat cgcccgcaat
     1141 atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc
     1201 gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaaggc
     1261 aaacgccgcg cgcacgcgct cttccaggat ttcctgcggg atttcatcac caataaacgc
     1321 cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt
     1381 caggctgaat tccggcaggt cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg
     1441 ggttacggct tgcgcaaagc tgacctgctc attgtgatct ttcagattgt agagtttcat
     1501 ttagttttcc agtactcgtg cgcccgccgt atccagccgg caaatatgaa caaaaccttc
     1561 ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt
     1621 gtcacacaga gcgaacaagg tcgggccgga gccggagata ccgctcgcta ccgcgccgat
     1681 ttccgcaacc gcctgccgcg cctgccggaa gccaggcagt aaccgttcac ggtagggttc
     1741 agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg
     1801 aatgaagcca gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg
     1861 taaaatagcc ctggcttctg ccgttgagac tttaatcccc ggatacgcca gcacccacag
     1921 ccactcatca aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg
     1981 cataccacca agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agattcgtcc
     2041 ttccagctcg cccatcaaag ccagcaaacg agtgtcatta agcggcttgc cacagtgttc
     2101 attcatcgcc atcagcgccg cgaccaccga acaggcgctg gagcctaagc ccgaaccgat
     2161 tggcatattc ttttccagag tcatcgccac tggaatttgc ttgccaagct cctggcaaaa
     2221 acgctcccag cactgataaa cgatattttc ccgtggttct gacggcagct tatcggcaaa
     2281 gcgtccgagg ttgttgagac tgaatgtctc tgccgcctca accgtgacta catctccgag
     2341 caatgcacca tcaacaggtg tcaccgccgc cccgagcaca tcaaacccga cgctcatatt
     2401 ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg
     2461 tacgtagcag atcggcaaag acaccggcag ctgtaacgtc attgcccgca ccatatccgc
     2521 gcagcaccaa cggcagcggc tgataatagt ggctataaaa ggccagggcg ttttcgccat
     2581 ttttcacttt gaacagcgga tcattaccat ccacttcggc aatcttcacg cggcaggcac
     2641 catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg gccttcgcca
     2701 cgcgcgcggc aaagagatcg tcgagctgtg acagattcgc cataaaagcg gcaacatcac
     2761 cctcagcgtt aaactctgcg ggcagcacag gttcaatttc aatatccgcc agctccagtt
     2821 cacgtcccgt ttcacgagcg agaatcaata gcttacgcgc tacatccata ccagaaagat
     2881 catctcgcgg atccggttcg gtataaccca tttcccgcgc cagagtagtc gcctcggaga
     2941 aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa agaatgccgg
     3001 agaacttcat caattcatca ccagcattga gcagattttg caggttctca ataaccggta
     3061 atccagcccc aacgttggtg tcatagagga atttacgccg cgatttttcc gccgcatgac
     3121 gcaacagatg gtagtaatcc atcgacgagg tgttggcctt tttgttcggc gtgacaacgt
     3181 ggaaaccttc gcgcaagaag tcggcatatt gatccgccac tgcctggctg gaggtgcagt
     3241 caacaatgac cgggttcagc agatgatatt ctttcacgag gcgaattaag cgcccgagat
     3301 taaacggctc tttggcttgc gccagttctt cctgccagtt ttccagattt aggccatgca
     3361 cattggtgag cagtgccttc gagttggcaa caccgcagac acgtaagtcg atatgtttat
     3421 tcttcagcca gctttgttga cgcttcagtt gctccagcag cgcaccgcca acgccaccga
     3481 cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc
     3541 gcacgccagt ggtcgcatca tcgttattta ccacgacaga gattgagcgt tcagaagatc
     3601 cctgagcaat ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg
     3661 agatcccacg caaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttccg
     3721 tcaccgccag cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg
     3781 cccgttcagc tcgcacacag tcgctttgtg gaacgcagaa actgatactg tattcggaag
     3841 atgattgcgt aatcagcacc acggaaatac gggcgcgtga cattgcagca aagacgcgcg
     3901 ccgccatgcc gaccattcct ttcatccccg gaccagaaac gctgaacatt gccatattat
     3961 tcagattgga aatgcctttg accggtaatt cgtcttcatc acggctggca ccaatgagcg
     4021 tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctgg aactgggcga
     4081 tgggggtaat ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt
     4141 cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat
     4201 agaccccgtc aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag gcagccagca
     4261 ccgccgcgga gtagtcggaa ccgttacgtc caagtaccac cagttcgcct ttttcattac
     4321 cggaggtgaa acctgccatc aacaccatgt gatcagccgg aatgcggctt gccgcaatac
     4381 ggcgggtgga ctcagcaata tcgacggtag attcgaggta atgccccact gccagcagtt
     4441 tttcgaccgg atcgataacg gtaacgttgt gaccacgagc ttctaacacg ccggccataa
     4501 tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttgatgcta tccgggcact
     4561 gccccaacaa actaatgcca tgcaggacat gttttatttg ggcaaattcc tgatcgacga
     4621 aagttttcaa ttgcgccagc gggaaccccg gctgggcggc ggcgagtccc gtcaaaagtt
     4681 cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt
     4741 tttcaatcat cgccaccagg tggttggtga ttttggcggg ggcagagagg acggtggcca
     4801 cctgcccctg cctggcattg ctttccagaa tatcggcaac acgcagaaaa cgttctgcat
     4861 ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg
     4921 tcgaaaaaaa gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag
     4981 cccgcaccgt tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc
     5041 atggatgttg tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa
     5101 gtatttagtg acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga
     5161 aactgctatc agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag
     5221 aatgaaaagc tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata
     5281 tcatgcagca aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgt
     5341 tgtaaccagt cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg
     5401 actcgttcgc gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgtcggt
     5461 tgttgtatta atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc
     5521 gaaggataat ccgccaccat cggcacacca gtaagaacgt cagccaacgc taactcttcg
     5581 ttagtcagcc cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat
     5641 ttttcctcta acagcagcac cagttctact ggcgtggcgt agtaatgata tttcgcccga
     5701 ctgcgcgcag tggtggcgac agtgaaatcg acatcgtgta acgattcaac cagtgtcggg
     5761 aaaactttaa tattatcaat aatatcacca gatccatgtg cgacccagcg agtggctggc
     5821 tccagatgtg cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc
     5881 cgcgccgctg ccccaatatt ttctgctctg gcgggtgcga ccagaataat cgttatacgc
     5941 atattgccac tcttcttgac caaatgaccg cgaacagggt gatcactgtc aacttattac
     6001 acggtgcgaa tttacaaatc cttaacgtaa gtcgcagaaa aagcccttta cttatcttaa
     6061 aaaaggctaa actatttcct gactgtacta acggttgaat tgttaaaaaa tgctacatat
     6121 ccttctgttt acttaggata attttataaa aaataaatct cgacaattgg attcaccacg
     6181 tttattagtt gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat
     6241 ttagatacga tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa
     6301 cgaagcgtag ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg
     6361 gttaggatga cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag
     6421 ctaatgatgt tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc
     6481 atgttacgcc gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact
     6541 tcctgtttcg atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat
     6601 cgttgaagac gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta
     6661 tgatgttttc gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat
     6721 caacctggtg atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga
     6781 actgcgcgag caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga
     6841 taaaattctc ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg
     6901 tgaactgacg attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag
     6961 cgaagaacgt cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag
     7021 ccgttcgttg atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc
     7081 catgcttcac ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa
     7141 aatgaccggc cgtgagctga aaccacacga ccgtactgta gacgtgacga tccgccgtat
     7201 tcgtaaacat ttcgaatcta cgccggatac gccggaaatc atcgccacca tccacggtga
     7261 aggttatcgc ttctgtggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaaac
     7321 ggcgcttttt agcgccgttt ttatttttca accttatttc cagatacgta attgatcgtc
     7381 cattgtaact tctttactgg ctttcatttt cggcagtgaa aacgcatacc agtcgatatt
     7441 acgagtcaca aacatcatgc cggccagcgc caccaccagc acactggttc ccaacaacag
     7501 cgcgctatcg gcagagttga gcagtcccca catcacacca tccagcaaca acagcgcgag
     7561 ggtaaacaac atgctgttgc gccagccttt caataccgcc tgcaaataaa taccgttcat
     7621 taacgcccca atcagactgg cgattatcca tgccacggta aaaccgatat gttcagaaag
     7681 cgccagcaag agcaaataaa acatcaccaa tgaaagcccc accagcaaat attgcattgg
     7741 gtgtaaacgt tgcgcggtga gcgtttcaaa aacaaagaac gccataaaag tcagtgcaat
     7801 cagcagaatg gcgtacttag tcgcccagtc cgttaattgg tattgatcgg ctggcgtcgt
     7861 cactgcgacg ctaaacgcgg ggaagttttc ccagccggta tcattgcctg aagcaaaacg
     7921 ctcaccgaga ttattagcaa accagctgct ttgccattgc gcctgaaaac ctgactcgct
     7981 aacttcccgt ttggctggta gaaaatcacc taaaaaactg ggatgcggcc agttgctggt
     8041 taagggcatt tcgctattac gcccggcagg caccacagaa agatcgccgg taccgcttaa
     8101 attcagtgcc atattcagct gcaggttctg cttccgccag tccccttcgg gtaaagggat
     8161 atgcacgccc tgcccgcctt gctctaaccc ggtaccgggt tcaatggtca gtgccgttcc
     8221 gttaacttca ggcgctttca ccacaccaat accacgcgca tccccgacgc taatcacaat
     8281 aaagggcttg cctaagatga tatttggcgc gttgagctcg ctaagacgcg aaacatcgaa
     8341 atcggctttt agcgttaaat cactgtgcca gacctgaccg gtataaatcc ctatcttgcg
     8401 ttcttccacg ttctgattgc catcaaccat caatgactca ggtaaccaaa aatggataaa
     8461 acttcgtttc cgctccacgg ttttatcctc ttcctgcacc gtataaagct cggtcacagg
     8521 aatagcgata agcggcccaa cgagtttttg cggcccgctg gtactttggc gaatcgcatc
     8581 ttccacatcg ctacggtaat cggcacgttc gacaatcact tgccgaatca gcattatcgg
     8641 aatcaacaac agcaatactg caccaaacaa ggtggtcatt ttccagaaca ggggggattt
     8701 caacatagtc ttctcctttg caatggcagc aggatacgaa ggctatgtgg gaagaatttg
     8761 aagctatgtg aagtgacggt gaagtcgaag cgaggccagc acgccacctt cctgcacgtt
     8821 gcgcagcgtg acttcgccgt taaacaaacg ggcgacctca ctgacgaacg ccaaccccag
     8881 accgctgctt ttttgcccat ttgcacgcgg caaagagtaa aagcgttcaa aaatacgtga
     8941 cagcgcgtag tcaggaatac cactaccggt atccagcacc ttaagggcga cgtgttcctg
     9001 atccacttcg gcgcttagcg ttatgcgacc gctctcgggg gtaaaatcga tggcgttatc
     9061 cagtaaattc cccagcgcct gctccagtaa cgccggttca gcagcaacat taacctcagt
     9121 gggcataaca tgtagagtga tgtttttttc tgccaactgc acggtgcgcg cttcgctgac
     9181 gcggcgaaat aatgccgcca catcaacaac agtcagaacg acttcctgac gattctccag
     9241 tcttgcctgg cgtagtaacg tttctaccaa tgcctgcata cgcgcatttt gcgtcagaat
     9301 gttgtcggta aaacgagcca ccacttccgg tggcggacct tcgcgtaaaa tttccgccgc
     9361 gccacgaatc gccgccagtg ggctttttag ctcatgggtt aacgcataaa catactgctc
     9421 aatatagttt ttcccttcca gcttcacgcg catactttcc agcgcctgcg caagcttacg
     9481 caactcgcta ctgccgagtt caggaagagg aacgggctta ttgtcagtga cggaatcggc
     9541 atagcgagtg agccgggcaa tggagcggtt gatccaccaa accatgcctg cgccaatcac
     9601 cagtgcaatc cccagcaaaa tggcgctggc ccataaaatt cgccgctcgc tacgcttaat
     9661 gaccggagcc atcgccgcgt tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc
     9721 gtccataatc ggcgcggcaa catacatcac cgaactttcg ggatcggcag gattttgcaa
     9781 cgtgctgcgc gcaccatact gaccacgcaa cgttagccag acgtcattcc agcgcgaata
     9841 atcctgccca acggctttat ttgccgaatc gaacaatact ttaccctgcg aatcggtcat
     9901 atagacatga tattcattgc gcactttgtt aatgccaccg atattggcgc gaaacggacg
     9961 atgttgtagc tgattaaacg cctgcgccag ttgcccatgc gttgggtccc cagagagcaa
    10021 atcgggacgc gccagctccg ccagcaacgt tgcggtgtcg atcaacgtcc cctccgttgc
    10081 tcttcgcacg cccggtttaa cttctttgac aaaaatagcc agtacgaacc aggccgccac
    10141 cgccaccagt aaaaaatagc ccagcagcaa ccgcatgccg atacgcatta caggcccctc
    10201 aggctatatc ccatgccgcg atgagtatta atcggtgaaa gatcggggtt gatggcacgc
    10261 agcttggcat gcaacgtttt aatgtgggta tcaacggtgc gatcgtaggt gtcctgcgca
    10321 tcttcccaca cgctatccat cagttgctgg cgggaccaga cgcggcctgg cgacttgagt
    10381 aacgtcttca gcaataaaaa ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg
    10441 atctgcgccg cgggttcatt caattcaaaa tgaccaatac ggatgacggg agacggcgac
    10501 gagaacttct tcacccgacg cagtaaggtg cgcaccctgg cgcacacttc gcggggtgaa
    10561 aacggtttag ccacatagtc gtcagcgcca atttccagcc caagcaggcg atcgacctct
    10621 tcacttcggg ccgtcaggaa cagtacaggt aacgccggat ggagcgccag taactggctg
    10681 cacaattcaa agccgctgat atcaggcaaa ccaacgtcga gaatcatgac gtcgggaacc
    10741 agctggcgag ctttatccag caccggcaag cctcgctcaa agacctcgac ggcaaaacct
    10801 tcctgctgca acatgtagac cagcgtgtcg gctatccctt gctcatcttc cactaaccag
    10861 accgtttccc gttgtataaa tcgcctctgt tattgccgcc acggcatgac aggaaccgcg
    10921 ctaatcgcgt ttttcggcga accttctaca actttgtcgg agtaagccag ataagcgagc
    10981 gcgttgcgtt tggcgtcata aaagcgcacg acctgtaacg acttaaagac cagggacgtg
    11041 cgttttttga atactacctc gccctgagct ttgccgtttt taatacgatc cgacagttca
    11101 atcggcccga cttgctgaca agaaatagcc gcatcggagg tatcttccgc cagacccaat
    11161 ccccctttaa taccacctgt tttcgcccgg ctcacataac aggtgacatt tttcacatcg
    11221 ggatcgtcaa aggcttccac aacaattttg tgatccgggc cgatcatttt aaatacggtg
    11281 tcgaccgaac caatctcttc tgcatgagcc aatggcccca gcattatcag gcttaaagac
    11341 aggatcaaat gcttgtattt catattgtta ccatttttat ctattacacg agcaattagt
    11401 tcgtcacggt aaataacata ttgattaaga acaaaatctc aattctttta tttccgtcaa
    11461 gccctaaaac atactctact aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta
    11521 ttatccaatt acctgatgtc aggtgctcgt tgttgaaagg atgaggatat tttatggatc
    11581 aggccggcat tattcgcgac cttttaatct ggctggaagg tcatctggat cagcccctgt
    11641 cgctcgacaa tgtagcggcg aaagcaggtt attccaagtg gcacttacag agaatgttta
    11701 aagatgtcac tggccatgct attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg
    11761 cggtcgcact acgcctgact gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg
    11821 actctcaaca gacatttacc cgcgcattca agaagcagtt tgcccagact cctgcacttt
    11881 accgccgttc tcctgaatgg agcgcctttg gtattcgccc gccgctacgt ctgggtgaat
    11941 tcacaatgcc agagcacaaa tttgtcaccc tggaagatac gccgctgatt ggtgttaccc
    12001 agagctactc ctgttcgctg gagcaaatct ctgatttccg ccatgaaatg cgttatcagt
    12061 tctggcacga ttttctcggc aacgcgccga ccattccgcc agtgctctac ggcctgaatg
    12121 aaacgcgtcc gagtcaggat aaagacgacg aacaagaggt attctatacc accgcgttag
    12181 cccaggatca ggcagatggc tatgtactga cggggcatcc ggtgatgctg cagggcggcg
    12241 aatatgtgat gtttacctat gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg
    12301 tatacggaac gtgcatgcca atgctcaacc tgacgcgccg taaaggtcag gatattgagc
    12361 gatactaccc ggcagaagac gccaaagcgg gagatcgccc aattaatctg cgctgtgaac
    12421 tgctgattcc tatccgccgt taacgctgca gctcatctaa tgcaggggcg tccagatgcg
    12481 agatgtcccc tgcggtttca acgacccagc cagacgccag ccacaggctt tcctgataat
    12541 ccacgcgcga aatagaacag ttacgcagac gtaagcggcg cgctgcccat gctggtaatc
    12601 cgagaatcgt actcaccaga catcccagtg caataccatg gctcaccagc agcggtcggc
    12661 ttccctgcgg taagtcccgg caagattcca gtgccgcatt gacgcgatcg ctgagctcct
    12721 gcattgactc gccttcagga atacgcccgt caacggtgcc attgaccagc tgccgacgcc
    12781 agttctcttc ttcttcggtc agagaatcga tatgtctttt ttccagcaca cccatgttta
    12841 attcacgcag gcgagaatca aagatgatgt cacagccgca ggcctgggcg atgatttccg
    12901 ccgtacgccg ggtgcgtcct aaatcgctac tgataatatg cgtaatgcca agctctttgg
    12961 cacgggttgc cacctgcatc gcctgttgct cacctttggc ggtcagcggg ctgtcagact
    13021 ggccctgaat acgtcgctcg gcgttccact gcgtttcacc gtggcggact aggtatacct
    13081 gtaacatgct ttttttccgt atactgctgt aacgttaatt ttctgagtaa tgctgattat
    13141 gcaccaagtt gtctgtgcga ccaccaatcc cgctaaaatt caggccattc tgcaggcatt
    13201 tcacgagatc ttcggcgaag gatcctgcca tattgcatcc gttgccgtcg agagcggtgt
    13261 accggaacag ccctttggca gtgaggaaac gcgcgctggc gcacgaaatc gggtagcaaa
    13321 tgcccgccgt ttacttccag aggctgattt ttgggtggcg attgaagctg gcatcgatgg
    13381 tgacagcact ttcagttggg tggtgattga aaacaccagc cagcgcggcg aggcgcgttc
    13441 ggctactttg ccgttacctg cggtgattct gcagaaagtg cgtgaaggtg aggcgcttgg
    13501 tcccgtgatg tcgcgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg
    13561 cgtgtttacc gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc
    13621 gctgagtccg tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc
    13681 cgcatccggc attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac
    13741 aaaatcaatc gcttttcagc aacacctctt ccagccactg gcgcagctcc acgggcgcgg
    13801 ctttcaggct gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta
    13861 actcacgctg actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca
    13921 acgcttcgcg ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt
    13981 ggtaggcatt cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca
    14041 tcgctgctga atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc
    14101 aaaatgcctc ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga
    14161 tcagtaacga cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata
    14221 acggtagtaa gcgtcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg
    14281 aatactctcg acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg
    14341 cacccgtcca ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg
    14401 ctgataaaca tattgcaggt aactggtgcc aatgttgata ttcgtttccg gatccagcaa
    14461 ttgcccaggg ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc
    14521 aggcataatc tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc
    14581 gctctcctga cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta
    14641 acgtttgaaa agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt
    14701 cccggcgatc gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc
    14761 cagttgagcc tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc
    14821 ggtgttatcg agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt
    14881 cagggcgctg tcaacattct gcggcgcttt atcaatcttc agctcatact cttctccgat
    14941 gcgttgtgct gcaaccatcg ggtagaaacc acgctgttgc atgagttgat gcaaaatctc
    15001 tttcgcttca gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc
    15061 atctttctct tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc
    15121 gccggtgcca agtgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat
    15181 aatggcgtca tcgcgccatt tcgcctgctc gtcggtgaca tcgttgccca tcaaacgcca
    15241 ggcgacgata tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag
    15301 cgacgggatc atcagccgtg cattttcagc atcctgccgc gccacactgg caaacgccac
    15361 cgctgccatt tgacgggtaa aatcggtcgc tccggttgta cgcgcgaagg tcagtaccgt
    15421 attagggttg ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat
    15481 ctgccctgcc agcactgtta ccaggccagt gttacccgct ttcatcgcca gacggatacg
    15541 ctctaaatac gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc
    15601 acaggcgtta ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc
    15661 ttcactctgc ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc
    15721 gggcttttcc gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt
    15781 gacgaaacga gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt
    15841 agtgaccgtc accgccggtt gattcatcag atcatcggtg atctggcggt attccaggta
    15901 gggataaagc ggataatcct tcagtccagg catcatttgt tcgaccacat ccatttgtcg
    15961 attatcccag gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc
    16021 tcgcgccacg ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac
    16081 ttgtttggct ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg
    16141 ccgcgtaata gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt
    16201 ttacttttac tgcgaaggtg atcgaccaac tgggattagg cggggaaaaa ggctacactt
    16261 cgcctttgaa aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc
    16321 gtttatacca tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac
    16381 atctctctga gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt
    16441 aagtctaccc tgctgcgcat tatggcaggc attgataaag acatcgaagg tgaagcgcgt
    16501 ccgcagccag acatcaagat tggttacctg ccgcaggaac cgcagctgaa cccggaacac
    16561 accgtgcgtg agtccattga agaagcggtt tctgaagtgg ttaacgccct gaaacgcctg
    16621 gatgaagtgt atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa
    16681 caaggccgtc tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg
    16741 gagcgtgcgg cggatgcgct acgtctgccg gactgggacg cgaaaatcgc taacctctcc
    16801 ggtggtgagc gtcgtcgcgt agcgttgtgc cgcctgctgc tggaaaaacc agacatgctg
    16861 ctgctcgacg aaccgaccaa ccacctggat gccgaatccg tggcctggct ggaacgcttc
    16921 ctgcacgact tcgagggcac cgtggtggcg attacccacg accgttactt cctcgataac
    16981 gttgcaggct ggatcctcga acttgaccgc ggtgaaggta ttccgtggga aggcaactac
    17041 tcctcctggc tggagcagaa agatcagcgc ctggcgcagg aagcttcaca agaagcggcg
    17101 cgtcgtaagt cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag
    17161 tcgaaaggta aagcacgtct ggcacgcttt gaagagctga acagcaccga atatcagaaa
    17221 cgtaacgaaa ccaacgaact gtttattcca cctggaccgc gtctgggcga taaagtgctg
    17281 gaagtcagca acctgcgtaa atcctacggt gatcgcctgc tgattgatga cctgagcttc
    17341 tcgatcccga aaggggcaat cgtcgggatc atcggtccga acggcgcggg taaatcgacc
    17401 ctgttccgta tgatctctgg tcaggaacag ccggacagcg gcaccatcac tttaggtgaa
    17461 acggtgaaac tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt
    17521 tgggaagaag tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc
    17581 cgcgcctacg ttggccgctt taactttaaa ggggttgatc agggtaaacg cgttggtgaa
    17641 ctttccggtg gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac
    17701 atgctgctgc tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa
    17761 aacgccctgc tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc
    17821 gaccgtatcg ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa
    17881 ggtaacttta ctgagtacga agagtacaag aaacgcacgc tgggcgcaga cgcactggag
    17941 ccgaagcgta tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgt
    18001 gaacgcctta tccggcctac aaaccaagaa atttcaatac gttgtgaatc tccaggcctg
    18061 ataagacgcg gtaagcgtcg catcaggcat tgagcaccga atgccggata cggcgtgaac
    18121 gccttttccg gcctacaata gccttagtat ccgcaatttc gtagcgggtc acccgtgctg
    18181 attcttttaa accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa
    18241 aaaacttatt ttatacctcg cctttgagcc gtttcatcgc ggttatctct gctcccccat
    18301 catctcccgc accagctcca cgcagcgcag gaaacggctg tcataatcgt cctcttcaac
    18361 ccgcacgaat tcgatattgt tctcttccag catctccacc agcaagttct ggaactcttt
    18421 gcgatccacc gaactgccga ggctgcgtaa accatccgcc acccacggcg tgttgttctc
    18481 cagcaggatc accagatcga aacggtattc atcaatcaac gcctgtacga acggatgctc
    18541 acgcccttcg tactttttgc agaacgcctg ggtagtgaca aaatcagtgt caataaacgc
    18601 cactttattg gcatatttca ctgcaaaatc aatgtattgt gcgtggccca gcgcgatttt
    18661 atcgtaatcg gaatactgca atgcgatctc atcaccgccg aggtgtgaaa agacataatc
    18721 gcgaccatat tcccacgcac tggtggtatt gaagatattg gcaagtttgt ttaccagggt
    18781 ggatttaccg ctcgactcgc caccaaggat cgccacggta cgtacaaaga acggcttcac
    18841 ttccgtagga atatattccc agtagcggaa cgggttttcg cggatctgcg caccgctgat
    18901 actcataaag gtacgtttcg gatcgaccag caccgtatcg atccccagat gttccatata
    18961 ctgtggcgca tcggcttctt ccgaggtgta gatcaggtcc ggctgaatcc ccttttcagc
    19021 cataaacttt ttgatgccgt tgctccacac atcccagccg tgcggatacg gctccatgcc
    19081 ctcctcgttg aaagcatgaa tgcgaatatt tttctgatat ttaaaagttt gtaataacca
    19141 gcgcagacga tccggcaccg tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg
    19201 gtcgcgggta tcgtcaaaac ccataatgat atgcagctcg tcaacctggc tacaggcgcg
    19261 ctggataagg tagatatgtc cggtatgcag cgggtagaac ttaccgaata cgacaccaat
    19321 ggttttcttc tgtcggggaa attcaagccc caaaaaacgg tgcaacgcct ccagcttttg
    19381 cgcgctgggg cttttaattt tggcattcag taactggctt aaataccctt tggtcatacc
    19441 gctggcatca gctacctgct gtagcgtgca gccctgttgc ttgatggcag ttttcaggta
    19501 atcaaatgac gacatatctc cctccgtatc tctcattata agtcgtcgaa cacgctaagc
    19561 gcgtcggaga gttttttaac gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg
    19621 ttagccgccg gaacaatcgc ccggcgaaaa ccgtgtttcg ccgcttctga aattcgttcc
    19681 tgaccgctgg gcaccgggcg gatctcccct gccagcccga cttcaccaaa caccaccaga
    19741 tcctgtggca gcggtctgtc acgcaggctg gaaaccatcg ccagcagtaa cgctaaatcg
    19801 gcactggttt cggttacctt cacgccgccg accacgttca caaatacatc ctgatcggcc
    19861 atttgcagac caccgtgacg gtgcaagaca gccagcagga ttgccagacg gttttgttcc
    19921 agccccactg ccacgcggcg cggattcgcc atcatcgagt gatcgaccag cgcctgaatc
    19981 tccaccagca acggacgcgt tccttcccac accaccatca cggagctgcc ggaggtcact
    20041 tcatctccgc gacttaagaa aattgccgaa gggttgctga cttcacgcag cccctgttcg
    20101 gtcatcgcga agacgcccag ctcattcacc gcgccgaagc ggtttttatg gctgcgcaag
    20161 gtgcgaaaac gggagtcggc atcaccatcc agaagcaccg aacagtcgat acagtgttcc
    20221 agcactttag ggccagccag cgagccatct ttggttacgt gcccaaccat gacaatcgcc
    20281 acaccgcgcg ttttggcgaa gcgcgtcaga taagccgccg tttcacgcac ctgcgccacg
    20341 ctaccaggcg atgattgtac atccgccata tgcatcacct ggatcgagtc aattaccatc
    20401 agtttcggtt gctcttcttc ggcaatcagg cagatctgtt cgatgctggt ttccgacaac
    20461 atattgagat tgtcagtcgg caggccaagg cgatgagcgc gcattgccac ctgttgcagc
    20521 gactcttcgc cagtgacata cagcgttttc atctgctggg ccagtttgca cagcgtttgc
    20581 agtagcagcg tggatttccc cgcacctggg ttaccgccaa tcagaatggc gcttcccggc
    20641 accacgccgc cgcctagtac gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc
    20701 tcttcaaggc tgatatcgga gagtttctgg acttttgcca ccccggcgct accggcatag
    20761 ccgctgagac gctcgttacg cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg
    20821 gtgttccagg catgacaggc actgcactgc ccctgccagc gcggataatc ggccccgcat
    20881 tcattacaaa caaaggcgcg ttttggagct tttgccacgg tgtacctcgt taatgctgtg
    20941 ccgcccgcag gatggcgggc gagcaattac ttctgattca ggctgccaga gaggatgcag
    21001 aataccccca tcaggtcagc gtgacggatg gtgacttccg ccttttcatt cacttttggc
    21061 ttggcatggt aggcaatccc cagccctgct gctttgatca tcggcaggtc attagctcca
    21121 tcgccaatcg ccacggtctg cgccagcggg atttcatact cctgcgcgag gcgagtcaaa
    21181 gttttcgctt tgtactgcgc gtctacgatg tcgccgatca cattgccggt aaatttaccg
    21241 tccatgatct ccagttcatt ggctaccacg gcggtcaggc gcagcttgtc gcgcagatat
    21301 tcagcaaaga aagtaaagcc gccggaggca atcgccactt tccagcccag cgtttccagc
    21361 ttgagcacca gttgcgttaa gcctggcatc agcggcagat tttcacgcac ctgttgcaga
    21421 atattggcgt cagcgccttt cagcgtcgcc acacggctgc gcaggctggc ggtaaaatcg
    21481 agttcgccgc gcatcgcccg ttcggttact tccgccacca tctcgcccgt tccggccagt
    21541 ttggcaattt catcaataca ttcaatctgg atggcggtgg aatccatgtc catcaccagc
    21601 aaacccggcg tgcgcaggtg cgggattttc cccagcgggg cgacatccag ttgcgcttca
    21661 tgggccaggc gcgtagcccg tgcggtgagt gaacctgcca gacgaatcac ctgataatct
    21721 tccacgcacc aggcggcaac aatcaccatc gccgcaccca gtttgctctg gtattgggtc
    21781 agacgctgtt tatccagccc acgaccatac agcagccagc cgctacgacc tgcgtggtaa
    21841 tccagtggca tcacttcatc accacttaat gaaagaggca gaccaggcca taaagagaca
    21901 tcttcaggca ggtcgcacca ggtaatgtta ggcattaagg atcctgtaaa atcgttcgaa
    21961 gcagggaaaa taacgcatga ggctaccttg tatccattgc ttctggcaac attaagtctc
    22021 aaattttcaa agggtggaag atggctcgca caaaactgaa attccggctg catcgggcag
    22081 tgattgtcct gttctgtctt gccttgttag tggcgctgat gcagggagcg tcatggttta
    22141 gtcaaaacca ccagcgacaa cgtaatccac agctggaaga actggcccgc accctggcgc
    22201 gtcaggtaac gctgaacgtt gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca
    22261 ttcaggcgat cctcgatcag ttaacggatg aaagccgtat cctcgacgcg ggtgtgtatg
    22321 acgaacaagg cgatcttatc gcacgttctg gcgaaagcgt cgaagtgcgc gaccggctgg
    22381 cgctcgacgg taaaaaagcg ggcggctatt ttaaccagca gattgtcgaa ccaattgccg
    22441 ggaaaaacgg accgctcggc tatctgcgcc tgacgctcga cacccatacg ctcgccaccg
    22501 aagcccaaca ggtggataac accactaaca ttttacgcct gatgttgctg ctttcactgg
    22561 caatcggcgt agtgctgacc cgcacgctgc tacagggtaa acgtacccgc tggcagcaat
    22621 cgcccttcct gttaaccgcc agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag
    22681 agtgacccat tactacaaga aaggaaatcg ttatgtccac attacgcctg ctcatctctg
    22741 actcttacga cccgtggttt aacctggcgg tggaggagtg tatttttcgc cagatgcccg
    22801 ccacgcagcg cgttctgttt ctctggcgca atgccgacac ggtagtgatt ggtcgcgcgc
    22861 agaacccgtg gaaagagtgt aatacccggc ggatggaaga agataacgtc cgcctggcac
    22921 ggcgcagtag cggtggcggc gcggtgttcc acgatctcgg caatacctgc tttaccttta
    22981 tggctggcaa gccggagtac gataaaacca tctccacgtc gattgtgctc aatgcgctga
    23041 acgcgcttgg cgtcagcgcc gaagcgtccg ggcgtaacga tctggtggtg aaaaccgccg
    23101 aaggcgaccg caaagtctca ggatcggcct atcgcgaaac caaagatcgt ggcttccacc
    23161 acggcacctt gctgctcaat gccgacctta gccgcctggc aaactatctc aatccggata
    23221 aaaagaaact ggcggcgaaa ggcattacct cagtgcgttc ccgcgtgacc aacctcaccg
    23281 agctgctgcc ggggatcacc catgagcagg tttgcgaggc cataaccaag gcctttttcg
    23341 cccattatgg cgagcgtgta gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc
    23401 caaacttcgc cgaaatcttt gcccgtcaga gtagctggga atggaacttc ggtcaggctc
    23461 cggcattctc gcatctgctg gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg
    23521 acgttgaaaa aggccatatc acccgcgccc aggtgtttac cgacagcctc aaccccgcgc
    23581 cgctggaagc cctcgccggg cgactgcaag gctgcctgta ccgcgcggat atgctgcaac
    23641 aagagtgcga agcgctgttg gttgacttcc cggaccagga aaaagagcta cgggagttgt
    23701 cgacgtggat agcgggggcg gtaaggtaac tcgctgatta aaataccaaa ggaagggaat
    23761 atgatcgatc tggaaaacca ggagcgggag attatcaatt taatgttaag ccagcgtatt
    23821 tcctggctag ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg
    23881 ttaggtatct cgattaattc tctaaaatag atcgaaaaga cggaacggtt tagctcaaac
    23941 atcaaaagca aaatggcaga aatttatggt tgtccaccag agttgctgat atgcccttcc
    24001 tggatgacgg ctgaacataa atgaatagat t
//
LOCUS       DADLTD010000044        23493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLTD010000044 DADLTD010000000
VERSION     DADLTD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.044"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(297..611)
                     /gene="pspE"
                     /locus_tag="JN701_001393"
     CDS             complement(297..611)
                     /gene="pspE"
                     /locus_tag="JN701_001393"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY7363897.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(686..907)
                     /gene="pspD"
                     /locus_tag="JN701_001394"
     CDS             complement(686..907)
                     /gene="pspD"
                     /locus_tag="JN701_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY7363898.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(916..1275)
                     /gene="pspC"
                     /locus_tag="JN701_001395"
     CDS             complement(916..1275)
                     /gene="pspC"
                     /locus_tag="JN701_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY7363899.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="JN701_001396"
     CDS             complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="JN701_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY7363900.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="JN701_001397"
     CDS             complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="JN701_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY7363901.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2388..3365
                     /gene="pspF"
                     /locus_tag="JN701_001398"
     CDS             2388..3365
                     /gene="pspF"
                     /locus_tag="JN701_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY7363902.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="JN701_001399"
     CDS             complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="JN701_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY7363903.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4788..6020)
                     /locus_tag="JN701_001400"
     CDS             complement(4788..6020)
                     /locus_tag="JN701_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7363904.1"
                     /translation="MPFDTLNESITCDVCVVGGGYTGLSSALHLAEAGFDVVVLEASR
                     IGFGASGRNGGQLVNSYNRDIDVIEKSYGMDTAPMLGSMMFEGGEIIRERIKRYQIDC
                     DYRPGSLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAIRREVASDRYTGALLDH
                     SGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVVRTAKGQVTAKYVIVAG
                     NAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNYCVEDCNYLLDYYRLTA
                     DNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDYRWTGNFLLTLSRMPQF
                     GRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAFANLPHYPFPGGRTLRV
                     PFTAMGAAYYSLRDRLGV"
     gene            complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="JN701_001401"
     CDS             complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="JN701_001401"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY7363905.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="JN701_001402"
     CDS             complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="JN701_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY7363906.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="JN701_001403"
                     /pseudo
     CDS             complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="JN701_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9380..10798
                     /locus_tag="JN701_001404"
     CDS             9380..10798
                     /locus_tag="JN701_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY7363907.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10947..11112
                     /locus_tag="JN701_001405"
                     /pseudo
     CDS             10947..11112
                     /locus_tag="JN701_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11102..12487
                     /gene="puuP"
                     /locus_tag="JN701_001406"
     CDS             11102..12487
                     /gene="puuP"
                     /locus_tag="JN701_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY7363908.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12621..12866
                     /locus_tag="JN701_001407"
     CDS             12621..12866
                     /locus_tag="JN701_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY7363909.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13179..14822
                     /gene="sapA"
                     /locus_tag="JN701_001408"
     CDS             13179..14822
                     /gene="sapA"
                     /locus_tag="JN701_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY7363910.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14819..15784
                     /gene="sapB"
                     /locus_tag="JN701_001409"
     CDS             14819..15784
                     /gene="sapB"
                     /locus_tag="JN701_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY7363911.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15771..16661
                     /gene="sapC"
                     /locus_tag="JN701_001410"
     CDS             15771..16661
                     /gene="sapC"
                     /locus_tag="JN701_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY7363912.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSEASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16661..17653
                     /gene="sapD"
                     /locus_tag="JN701_001411"
     CDS             16661..17653
                     /gene="sapD"
                     /locus_tag="JN701_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY7363913.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17655..18461
                     /gene="sapF"
                     /locus_tag="JN701_001412"
     CDS             17655..18461
                     /gene="sapF"
                     /locus_tag="JN701_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY7363914.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18529..18882
                     /locus_tag="JN701_001413"
     CDS             18529..18882
                     /locus_tag="JN701_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY7363915.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19250..20038
                     /gene="fabI"
                     /locus_tag="JN701_001414"
     CDS             19250..20038
                     /gene="fabI"
                     /locus_tag="JN701_001414"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY7363916.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20183..21310
                     /locus_tag="JN701_001415"
     CDS             20183..21310
                     /locus_tag="JN701_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY7363917.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21378..23312
                     /gene="rnb"
                     /locus_tag="JN701_001416"
     CDS             21378..23312
                     /gene="rnb"
                     /locus_tag="JN701_001416"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY7363918.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23390..23493)
                     /locus_tag="JN701_001417"
                     /pseudo
     CDS             complement(23390..23493)
                     /locus_tag="JN701_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5307 a         6208 c         6120 g         5858 t
ORIGIN      
        1 gacgcgttac aagtttctgt aaatgagttg cggtaaatgt tccaccgtag atttcgtcca
       61 ggtaatcagt aattttctgt ttcatgttgc ctttcagtgg tagcgatccc atgaaacgag
      121 agactactcc gcagaaaata aactgtcaat tgagcacaag ggttttagtc acgcaggaaa
      181 tcatttttta ccagggaaaa agcgtatttg tgagctgacg caaataatct atggggatgt
      241 aaataaggtg tgacagaaaa aaaacggcgc ataagcgccg ctcatggtga attcttttaa
      301 cctttgacct tcggcattgc gatgtctttc aagccaccgg cgttctcaac gtgggtatat
      361 cccatctcgc taaggatctc ttttgcttgc cctgactggc gtccggcatt gcaatacact
      421 ttcacggtgt cgtttttatc cggaacggcg gtggcaatgc gctctttcac ttccttcagg
      481 ggaatattga tggccccctg aacgtgctct tgctgatact gctctggaac acgaacatcg
      541 atccagtgtt cagcggcgaa aacgggcagt gaaaacacca gtgccagagc aagtaagcct
      601 tttttaaaca tgtaagcgtc cttctatgga tgaaaattgc catccattat acgcctgaga
      661 tttaacaatt tttgcgaaac tcccctcacc ttttataacg ctgtgccagt ttattagcag
      721 cccgacttaa cagcggttcc agcgccacag ccagcaacat tttcagcggt cggcgagcaa
      781 ctgattttat cgcccagccc gctacacccg ccgggccata gcgcagtgcg gtaagaagta
      841 ccagcttgcc tgctaatttg aaaccaggct ttaccttttg cccggcctgt tgccagcgag
      901 tattcataac tttcctcaca gttgacggaa acggctacgt aacgtgaaag tatcggaagt
      961 gacataacgt tccatctcgc gtaaacgcgt ttcacttgcc gccagttcac ggtcgacttc
     1021 atccagcaat tcgctgctgg agggtagctg ctcaccaaag gccatgttgt ccggcattgg
     1081 atcaagcgca aatgacaaaa tgatgtaagc aaccagggta aacagcgcca gaccgaagaa
     1141 aatcgacagc accaccagga tacgcaccag ttttaccggt acatcaaaat agttggcaat
     1201 cccggcgcag acgccgcgga ccatgccctg ctgtggaata cgccataatt ttttattgag
     1261 attaatgccc gccattagcg atccctccag ttcggatgtt ctgcatcaag aatagattcc
     1321 agcgcctgaa tacgttcgcg catccgtttt gcttcatcag ccagttgcgc taatcgctgc
     1381 tgctcacttt gcgacaattc actgcgacca gaacgattgc tgtaatgcag ccataaccag
     1441 atcggtaaaa caaacagcac aaaaatggtt aacggaatag ccagaaatag cgcgctcata
     1501 agtactcctt agacgatgag cggcggcgcg ttcggacgcc gcctagatgt tattattgat
     1561 tgtcttgctt cattttggct tttaattgtg ccagttgttc gctgattgca tcatcggctt
     1621 tcagttcggc aaactgatcg tccagcgatt tttgtttacc gaagctgtgg ctttctgctt
     1681 ccgcttccat ctggtcaata cgacgttcga aagattcgaa acgagccatt gcttcatcca
     1741 gtttgccact gtccagctga cgacgcacat cgcgcgacga gtttgccgcc tgatggcgta
     1801 acatcaatgc ctgctggcga gcgcgtgttt cgctcaattt gttttccagc tcaccgctct
     1861 ctttcttcat gcgtgccagc gtatcgtcca ccagcgtcac ttcatgttcc agactcttaa
     1921 tcagatcggt cagtttctgt ttttcaatta acgccgcacg tgccaaatcc tctctctctt
     1981 tcagcagcgc cagttcggct ttttcctgcc attcaacttc acgcgccgac gcttgttcaa
     2041 tacggcgagt cagctgtttc ttttctgcca acgcacgcgc cgaagtagaa cgtacttcaa
     2101 ccagtgtatc ttccatctcc tggatcatca gacgcaccag tttctgtgga tcttccgctt
     2161 tctctaacag agcgttgatg ttggcattca cgatgtcggc aaagcgagaa aaaataccca
     2221 taatgttgtc ctcttgattt ctgcgttcag gattgtcctg ctgaactgtt aatacaattt
     2281 gcgtgccaat tttttatctt tttgatttat aaagatctga ttgaagaatc atcagcaaca
     2341 tgccaggatg aattagcgaa ttacactaac aagtggcgaa tttcatcatg gcagaataca
     2401 aagataattt acttggtgag gcgaacagct ttctcgaagt gctggaacag gtttcgcatc
     2461 tcgcaccgct ggacaaaccg gtgctcatca tcggcgaacg cggcaccggt aaagagctga
     2521 ttgccagccg cctgcattat ctctcctccc gttggcaagg gccgtttatt tcccttaact
     2581 gcgcggcgtt aaatgaaaat ctgctggatt ccgaactgtt tggtcacgaa gcgggggcgt
     2641 ttaccggtgc gcaaaaacgt catccaggga gatttgaacg tgccgacggc ggtacgctat
     2701 ttcttgatga actcgctacg gcaccgatga tggtgcagga gaaattattg cgcgtgattg
     2761 agtacggtga actggagcgc gttggcggca gccagccatt gcaggtgaat gtgcggttgg
     2821 tatgcgcgac gaatgccgat ctcccggcga cggtcaatga aggcactttt cgcgctgacc
     2881 tgctcgaccg actggctttt gatgttgtac aactgccacc actgcgcgag cgcgaaagcg
     2941 acataatgtt gatggcagaa cactttgcca tccagatgtg tcgggaaatc aagctgcctc
     3001 tgttcccggg gtttacggag cgcgccagag aaacattgct gaattatcgt tggccgggaa
     3061 atattcgtga attgaaaaac gtggtggaac gttcagtgta tcgccacggc accagcgatt
     3121 atccgcttga tgacatcatt attgatccct ttaaacggcg tccgcctgaa gacgctatcg
     3181 ccgtttcaga aaccacctcg cttccaacac tgccgctgga tttacgtgag tttcagatgc
     3241 agcaggaaaa agagttgctg caactcagtt tgcaacaagg gaaatataac cagaagcgcg
     3301 cggctgaatt actggggtta acttatcatc agttccgcgc gttgttgaaa aagcaccaga
     3361 tttagcgaag ttgagcaata caacttgccg gatgcggcgt gaatgcttta ttcggcatac
     3421 ataatcaata agtcgtttgc aggcctgata agcgcagcgc atcagacatt attgcgttgg
     3481 gctattaatc gctcagcgca tcctgcaaaa ttttcattgc cgcatcgaat tgcgcatccg
     3541 gaatggtcag cggatacagg aagcgaatca cgttgccgta tgcgccacag gtcagcagga
     3601 gcagcccctg cgccagcgcg cgttgctgga ttttctgtgc aatcgccgct gacggctcgc
     3661 ccgtttgcgg atcgttaaac tctgccgcaa tcatcgaccc caggccgcgt accgcagcaa
     3721 tggccggaac gctttctttg gcatcaatca acgtgttttt gagacgctgg cccagttgat
     3781 tcgcgcgttc gcagagtgat tctttgtcga taatattgag caccgcgtgc gcggcagcca
     3841 ccgccagcgg gttcccggcg taggtgccac ccaacccgcc cggcgcgggc gcgtccataa
     3901 tattcgcgtt accgaccacg cccgaaagcg gcatcccgcc cgcgaggctt ttcgccatcg
     3961 tcattaagtc cggcttatcg gcgtaatgat ccatggcaaa cagtttaccg gtacgcgcaa
     4021 agccgctttg cacttcatcg gcaatcatca caataccgtg ctcgtcgcac aggcggcgaa
     4081 tggcggcaac cagctctttt ggcgcaacgt tgaaaccgcc ctcgccctgc accggttcga
     4141 aaataatggc cgccacctgc ttcgcttcaa tgtccgattt aaacaagcgt tcgatggcgt
     4201 cgagggagtc ctgtgttgaa atgccgtgta aatctgacgg ataaggtacg tgatacaccg
     4261 aaccagggaa cgggccgaag ccgattttgt acggcgcaac ttttccggtc agcgccatgg
     4321 tcatatatgt acgaccgtgg aagccgccgc taaacgcaat cacgccaggt cgtccggtat
     4381 gggcgcgagc aattttcacc gcgttttcca ccgcttccgc gccggtagtg aagaatgcgg
     4441 ttttagcctg cccgctcact ggggcaaggg cgttaatttt ctccgccagc gtgacgtagc
     4501 tttcgtacgg cacaatctga tacgccgtgt gggtaaactg ttgcaattgc tgctccaccg
     4561 ccgcgaccag atcagggtgg cgatgcccgg tattcagcac cgcaatgcct gcggcgaaat
     4621 cgatgtattc gttgccctct acgtccttca gcgtagcgtt ttctgccgac tgggcgaaga
     4681 agttacacat cacgccaacc ccgcgtgggg tagcagaaag acgacgctga tggaattcat
     4741 tgttgctcat gtttcacacc ttttgactct tcactgttaa tcggaaatta aacgcccaga
     4801 cgatcgcgca ggctgtaata agccgcgccc atcgcggtaa atggcacgcg cagcgtgcgc
     4861 ccgccgggga acgggtaatg cggcagattg gcgaaggcat cgaaacgctc ggcgtcaccg
     4921 cgcagcagtt cagcaatcaa acgcccggcg aggtgggtac aggtgacgcc gtggccgctg
     4981 tagccctgca tgtaatagat gttggtatcg aggcgaccga actgcggcat ccgtgaaagg
     5041 gtcagcagga agttgcccgt ccagcgatag tcgattttga cgcccttcag ctgcgggaag
     5101 gttttcagca gtttcggcac aacaagacgc tcaacgtcat ccgggtcgcg cgcgccgtag
     5161 accacgccgc cgccgtacag caaacggttg tcggcggtaa gacggtagta atcaagcaga
     5221 taattacagt cttccacaca gtagtttttc gggatcagcg aacgggctaa atcttccgac
     5281 agccgttcgg tggtgatcac ctgcgtgccg cacggcatgc tgcgtttcgc cagttccggc
     5341 tctactttat cgcccagata cgcattcccg gcgacaatca catacttcgc cgtcacctga
     5401 cctttggcag tgcgcacaac ggctggcgtg gtgtgctgga tctgcgtcac ggcagaaagt
     5461 tcatacacgc gcccgccgtt gaggcggatg gcgtccgctt cgccaatggc gaggtttagc
     5521 gggtgaatat gcccaccgct gtgatccagc agcgcaccgg tatagcgatc gctggctact
     5581 tcacggcgaa tggcgttggc gtccagcaat tccagctgtt tattgccgta gcgttcccag
     5641 ttctctttct gctcttcaag tgtggcgagc tgtttatcat tcatcgccac aaacaggctg
     5701 ccggggcggt agtcgcagtc aatctgataa cgtttgatac gttcgcggat gatctcacca
     5761 ccttcgaaca tcatgctgcc gagcataggg gcggtgtcca tgccgtagct tttttcgatc
     5821 acgtcgatgt cgcggttgta ggagttcaca agctgcccgc cattgcgccc gcttgcgcca
     5881 aagccgatgc gtgaggcttc gagaaccact acgtcaaagc ccgcttccgc cagatgcagc
     5941 gcggaggaga gcccggtata gccgccgcca accacgcaaa cgtcgcaggt gattgactca
     6001 ttcagggtgt cgaatggcac gtatttatgc gtaataactg ctggtatgtt cggtcatttc
     6061 aggcctccag gcttatccag atggttttca gttcggtgaa tttttcaagg gcatgcaggg
     6121 atttgtcgcg accgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt
     6181 cgttgtagtt attgacgaag acggaaccgg ctttcaggcg gcggctcata cggtgcgcgc
     6241 gggagaggtc gcgcatccat accgccgcgc caaggccgta ctggctgtcg ttggcaagct
     6301 gtagcgcctg ttcttctgat gtgaaacgcg tgaccaccag caccggaccg aaaatctctt
     6361 cgcggcttaa ggacgcattc gggtctacat ccacaaagat ggtcgggccg atggcggcag
     6421 ccagcccggc gttacggcca tccagcagca gttgcccttt gccttcgccc tcacgaataa
     6481 agctatggac cgagtcggcg tgggcgcagt cgattaaagt gcccatggtg gttgcggggt
     6541 caagcggatg gcccggctgc cagttttgcg cctgctgttt taacagggct aagaattcat
     6601 cggcgatgct ctcttccagc aacaggcgtg ttccggcgat gcacacctga ccctggttgt
     6661 agaagatgcc tgctgcggtg gagctcgccg ctttttgcag atccgaacaa tcggagaaaa
     6721 cgatgttggc gcttttgccg cccgcttcca gccagacgcg tttcatgttg ctgtcgcccg
     6781 catctttcag cagctgtttc ccggtacggg ttgaaccggt aaaggcaatg gcgtcgatat
     6841 cgttatgacg cgacagcgcc tgccccgctt catgaccaaa acccgtcacc acgttcaaca
     6901 caccatccgg caagcctgct tctttcgcca gccccgcgag acgaatcgca ctgagcggtg
     6961 atttttcaga cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc
     7021 agcaagtcag caacagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt
     7081 cacgcacgat catcgccagc tcatggctac tggtggtcgc cacttcgcca tacactttgt
     7141 cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca tcacgcagac
     7201 tgtgacgaat cggtttgccg gtgtcgagag tttccagcag tgccagctct tcggcgtggg
     7261 cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga gaagagagtg
     7321 accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg atatcgacgc
     7381 tcttgccgcg ggcaattttc gccagcggtg cctgggtgac tggatcaacg gtttcaaagg
     7441 tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt tcaatggcga
     7501 gacttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg actcctgttt
     7561 cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc cgggccgtat
     7621 gcccgtttat ttctggcgac attctttata tacacttcat ccttcaagcc gcctctgcct
     7681 tggctgcgtt cgctcccccg aatcacttac ctgagtaagc tcatcgggat tcgctcactt
     7741 gccgccttga tgcaacttga atgattttgt gtatagattg ccgggcgcat tgcgcttacc
     7801 cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa
     7861 atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc ataactttga
     7921 cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc ttccagtact
     7981 gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc cggctggtac
     8041 gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag cttcattgac
     8101 acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg cggctcatca
     8161 ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt cagcagcttt
     8221 tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat agcactgtga
     8281 gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc
     8341 gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc ttaaactgta
     8401 gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata aagccctcga
     8461 acaatatacg cgaaagagcg tactcgctac tgttccattc cgggtgccac tgtacgccca
     8521 gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc gagcgagctt
     8581 caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgtagagag tttacccaaa
     8641 agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc tgaacttcat
     8701 gcgacggtgc atattgctgt tccaccggca gttcggatct tcccgatgtt ccagcaattc
     8761 aggctgttcg cacagcttgc gatgcaacga accaccggtt gccaccacca gttcttgtaa
     8821 accccggcag atggcgaaaa tggggatgcg cctttcgagt gcggcattaa ttatcgccat
     8881 gctcagaaga tcacgcccgg gatcggcgtc aggctcatcg ccgttttcac catataggtg
     8941 cggctgcaca ttgctgggac taccaggaag ataaatgcca tcgagtttcg gcaaaagttg
     9001 ttcaagtaac gacggttccg ccagcgcatg tggtagcgca attggcaagc cgcctgcgtg
     9061 gatgatggca ttcaggtact tttcttgcag agtctgggtc gcatgaccct taagcctgtt
     9121 cctgcacatt acgacaccga taaccggatt gttcattata tttttcatgc tcaatctcac
     9181 aaagtggact aaattatcgc cattactgcg ctttaaatga cctttatgtt caatattttt
     9241 tcaatctagc agtgggtttt catttttgca aactaaattt aacatttgga aaacatttag
     9301 tttgcataca gattcgaatg gtggtcatta tattttacgc tttgataacg agcggaaaac
     9361 aaaccaaagg cgaagaatca tggaaaccaa tatcgttgaa gtagagaact ttgttcagca
     9421 gccagaagag aggcggggta gcgcctttac gcaggaagtg aaacgctacc tggagcgcta
     9481 cccgaatacg caatatgttg atgttttact tactgatttg aatggttgct tccgtggaaa
     9541 acgtatccct gtttcaagcc tgaagaagtt ggagaaaggg tgttatttcc cggcctcggt
     9601 gtttgcaatg gatattctgg gcaacgtagt agaagaagcg ggtctgggtc aggaaatggg
     9661 cgagccggat cgtacttgtg ttcctgttct cggttcctta accccttctg ccgccgatcc
     9721 agagtttatc ggtcagatgc tcctgaccat ggtcgatgaa gatggcgctc cctttgacgt
     9781 tgagccgcgg aacgttctta accgcctgtg gcagcagcta cgccagcgcg gattgttccc
     9841 ggttgtagcg gtagagctgg agttctattt actggatcgt cagcgcgacg ctgaagggta
     9901 tctgcaaccg ccctgcgcgc ccggcaccga taaccgcaat acacaaagcc aggtttactc
     9961 cgttgataac ctcaaccact tcgccgacgt gctcaatgat attgatgaac tggcgcagtt
    10021 acagttaatt cccgccgacg gtgcggtcgc cgaagcctcg ccgggtcagt ttgaaatcaa
    10081 cctttaccat accgataacg tgctggaagc ctgcgatgat gcgctggcgc taaaactcct
    10141 ggtgcgtctg atggcagaaa agcataagat gcactccact tttatggcga agccgtatga
    10201 agagcacgcg ggcagcggga tgcatatcca tatcagtatg caaaataatc gtggcgagaa
    10261 tgtgctttct gacacagaag gcgaagattc gccgctgctg aaaaagatgc tcgccgggat
    10321 gattgacctg atgccgtcgt cgatggcgtt gctggcacca aacgtgaact cgtatcgccg
    10381 cttccagccg ggaatgtatg tgccgacgca ggcgtcgtgg ggccataaca accgcaccgt
    10441 cgccctgcgt attccgtgcg gcgaccgtca taatcaccgc gtggaatatc gcgtggcggg
    10501 tgccgatgcc aacccatatc tggtgatggc agcgattttt gccggtattt tgcatggcct
    10561 tgataacgag ctgccgttgc aggaagaagt cgaaggcaac gggctggaac aggaaggctt
    10621 acccttcccg attcgccaga gcgatgccct gggtgagttt atcgagaatg atcacctgcg
    10681 ccgctattta ggcgaacggt tttgccatgt gtatcacgcc tgtaaaaatg atgagctgtt
    10741 gcagtttgag cgcctcatca cagaaacaga aattgagtgg atgttgaaaa acgcatagta
    10801 ccccttgcgg ggttgtttca tcgacctgag cggagccagg cctgacattt gctggtaatc
    10861 cagacatttc ccggatgtcg cgttatggag cgcaggcggc aacgggcttc cgttaacgat
    10921 taagactgtg acgaggaaat gagatgatgc cgatgattaa atccccgcat ggcgagggag
    10981 gctgcgtatg cgcccctcct gctactgact ggactccgtc ccctttgctt cccttgctaa
    11041 acaggtttga tttcaggtcg acacgaccgc aaaccttatt acgcaggggg aggcagcaat
    11101 tatggctatt aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc gtctgcgaaa
    11161 atcactgaaa ttgtggcagg tggtgatgat gggtctggcc tatctcacgc cgatgaccgt
    11221 atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg cgtcctattt
    11281 gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt
    11341 tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc acgtcggatt
    11401 tatggtcggc tggtcatcgc tgctggatta tctctttttg ccgatgatca acgtcttgtt
    11461 ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt gggtagtaac
    11521 cttcgtcgcc attttaaccg ccgcgaatct gaagagcatc aacctggtcg ctaacttcaa
    11581 taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc tggtagttca
    11641 gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt ttatcagcga
    11701 gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct ccttcctcgg
    11761 tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg tgatcccgaa
    11821 agccatcttc ctgacggcgg tctacggtgg cgttatcttt atcgcggcgt cgttctttat
    11881 gcagctgttc tttcccgata tcagccgctt taaagatccg gatgccgcac tgcctgaaat
    11941 tgcgctgtac gtcggcggca agctcttcca gtcgattttc ctctgcacca cgtttgtgaa
    12001 cacgttagcc tctggcctgg cttcacatgc cagcgtgtcg cgtctgctgt atgtgatggg
    12061 gcgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccaaaat ggcggactcc
    12121 ggcactcaac gtcattatgg tcgggattgt cgcgttgtcg gcgctgttct tcgatttagt
    12181 taccgccaca gcgttaatta acttcggtgc tctggtggcg tttaccttcg tgaatctgtc
    12241 ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg atcacttcca
    12301 ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg ttaacctcga
    12361 gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat atttatggta
    12421 tctgatccgc cgctatcgca aagtgccgtt gtatgatggt gacagaacgc cggtgagtga
    12481 aacgtaatag tcggatgcgc ggcgcattat cctgccggag ggtaatgcac cgcgccatta
    12541 actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta tgcttagcgc
    12601 attaacccca ggggcaaata atgaaccacg atatcccgct aaaatatttt gatattgccg
    12661 atgagtacgc gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc
    12721 actacttcca gttactgctc acccgtttga tgaacaatga agagatcagc gaagaagccc
    12781 aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag atcgcagagt
    12841 ttttgaatca atggggtaat gagtaattat ttaattgcag acgatcggtc agtatcgttg
    12901 tccggataaa gcgttcacgc cgcatccggc taacaatgac acgcactgca gcgtgcttta
    12961 atgggtttct cacgatcgta ccgctctgct cctcgctaag ttgttctgtg agttatagtt
    13021 atagaggaag cacgggtacg aatgttgaac agatggcgac gccacctgtt caacgaccaa
    13081 ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt cattaaatat
    13141 tagtaagata gcctctttac ataaaatccc ttaatattat gcgccaggta ttatcgtctc
    13201 ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc
    13261 atgctgatat ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc aacaccttta
    13321 acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag ttttatgatc
    13381 gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc gaaagctggg
    13441 aagtactcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa
    13501 aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg tttaccttcc
    13561 agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac ttcccctact
    13621 tcgacagcct gcaatttgcc gataacgtga aaagcgtccg caaactggat aatcataccg
    13681 ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctcgca acccattatg
    13741 cttcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgc caggagcaac
    13801 tcgaccgtca accggtcggc accggtccgt atcagttgtc ggaataccgc gccgggcaat
    13861 ttattcgcct acaacgtcat gatgacttct ggcgcggtaa accgttaatg ccgcaggtag
    13921 tggtggattt aggctccggc ggcaccggac gtctgtcgaa actcctgacc ggggaatgcg
    13981 acgttctggc ctggcctgct gccagccagc tatccatttt gcgtgacgac ccgcgcttgc
    14041 gtttaacgct gcgtcctggg atgaacgtcg cctatctggc atttaacacc gccaaaccgc
    14101 cgctaaataa tcccgctgtc cgccatgcac tggcactggc gattaataac cagcgcctga
    14161 tgcaatccat ctattatggt acggctgaaa cggcggcctc tattttaccg cgcgcctcgt
    14221 gggcctatga caacgaggct aaaattactg aatacaatcc ggcgaaatcg cgcgaacagt
    14281 tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtgcccaca cgttcgcagg
    14341 cgtggaaccc cagtccactg aaaactgccg aactgattca ggcggatatg gcgcaggttg
    14401 gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcgcgg ttgatggata
    14461 tgagccatga tctgacgtta tccggttggg cgacggacag taacgacccg gacagtttct
    14521 tccgtccttt actgagctgc gcggcaattc attcacagac caacctcgcc cactggtgcg
    14581 atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg gcggcgcgta
    14641 ttgaagccta tgacgaagcg cagagtattc tggcgcagga attgcccatt ttgccgctgg
    14701 cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa aggtctggta cttagcccgt
    14761 ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg aaaaaaccat
    14821 gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc tgctgacctt
    14881 cgttggcttc agcttgagct atttcacccc tcatgcgccg ttacaaggcg cgtcactgtg
    14941 gaatgcctgg gtgttctggt ttaacggcct gatccactgg gattttggtg tttccagcat
    15001 taacggtcaa ccgattgccg aacagttaaa agaggtcttc ccggcgacga tggaactgtg
    15061 catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga ttgccgggat
    15121 tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg gcttttcaat
    15181 cccggtgttc tggctggcgc ttctgttgac gctgttttgt tcactcacgc taggttggtt
    15241 gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg gttttgcgtt
    15301 gattgatgcc tggctttcgg actcaccatg gcgggatgaa atgatcatga gcgcaatccg
    15361 ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag tgatccgcct
    15421 gatgcgtatc agcactatcg aagtgtatga ccagaactat gtaaaagcgg cggcgacccg
    15481 cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg cataacgcgt taccgccggt
    15541 tattcctcgc ctgggcttac agttttccac catgctgacg ctggcgatga tcaccgaaat
    15601 ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc agcaggatta
    15661 cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg ttaacgtgat
    15721 ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt atgccttacg
    15781 atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt
    15841 atagtgaggc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct gtactgtgta
    15901 tttttggcgg ctggtttgcg ccttacggca tcgaccagca atttctcggt tatcaattat
    15961 tgccgccatc atggtcgcgc tatggcgaag tttctttctt cctggggact gacgacctcg
    16021 gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt ggcgcatttg
    16081 tggtgacgct tgccgcgacg atctgtggcc tagtactggg caccttcgcc ggggcgacac
    16141 acggcctccg ctcagcggtg cttaaccata ttctggatac cttactggcg atcccttcgc
    16201 tgctgttggc aattatcgtt gtagcgtttg ccggaccgag tttgtcccac gctatgtttg
    16261 ctgtctggct ggcattgctg ccgcgtatgg tacgttcgat ttacagcatg gtgcatgacg
    16321 aactggaaaa agagtacgtt atcgccgccc gtctggatgg cgcatcaacg ctgaatattc
    16381 tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc acccgcgcac
    16441 tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc gcacaactcc
    16501 cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat gtcgccccgt
    16561 ggactgtcat gctgccaggt gcggcaatta tgattagcgt gttgttggtt aacctccttg
    16621 gcgatggcgt tcgtcgtgcg attattgcgg gggtggaata atgccattac tcgatattcg
    16681 taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg accgggtaag
    16741 tatgacctta accgaaggtg aaatccgcgg tcttgttggt gaatccggtt ccggcaagag
    16801 tttgattgcg aaagcgattt gtggggtgaa taaagataac tggcgtgtta ctgctgaccg
    16861 tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc gcaaactggt
    16921 tggccataac gtgtcgatga ttttccagga accgcagtcg tgtcttgacc cttcagaacg
    16981 tgtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc gttggtggca
    17041 gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg ggattaaaga
    17101 tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggtgaat gtcagaaagt
    17161 gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg aaccgaccaa
    17221 ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc tcaaccaaaa
    17281 cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc aatgggcgga
    17341 taaaattaac gtgctttact gcggtcaaac agtggaaacc gcgccgagta aggagttggt
    17401 gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag acttcggcag
    17461 cgcgatgccg cataaaagtc gcctcaatac gctgcccggc gctatcccac tgctggaaca
    17521 gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag aatgcattgt
    17581 gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc cgctgaacat
    17641 ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc
    17701 gctaccggac cggctggttt cgccgtcaga ccgtagaagc ggtaaaaccc ttgagcttta
    17761 cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt aaatccacgc
    17821 tggcaaaaat gctggcggga atgatagagc ctaccagcgg cgagttattg attgacgatc
    17881 atccactgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg atttttcagg
    17941 atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aattctggat tttccactgc
    18001 gcctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa acgatgcgta
    18061 tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca cccggacaaa
    18121 aacagcgtct gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt attgccgatg
    18181 aagcactcgc ttcactggat atgtcgatgc gttcgcagtt gattaatctg atgctggagt
    18241 tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga atgatgaagc
    18301 acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg
    18361 cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgattgcc gggcactttg
    18421 gcgaagcatt gacagcggat gcgtggcgta aagatcgcta attttctgaa tcctccttcc
    18481 agattatcca cctcattctg acaagagatt aaatcttgtc agaatgaggt gatggataaa
    18541 ataaaatcaa atgcgcgtga tttaagacgc aatctcactt tgcaggaacg aaagctctgg
    18601 cgatatcttc gcagccgacg ttttggtgat ttcaaatttc gccgtcaaca tccggtgggg
    18661 agctacattc tcgattttgc ttgctgctcg gcgcgcgtag tcgttgagct ggatggtggg
    18721 cagcatgatt tggcagttgc ctatgatacc aggcgcacta gctggcttga gtcgcagggc
    18781 tggaccgtgc tgcgtttctg gaataacgag attgattgta atgaggaggc ggtgctggag
    18841 attattctgc aggaactgaa ccgccgatca ccctcaccct gaaagggcga gggggccgac
    18901 cgagccgaat agctgttgtg gtgaaaacat ggagacagtg ctggagaata ttcggcaagg
    18961 tctgaaccat cccagccatc gccataaaag ggttaggggc tgtatgagcc tgtttgttgc
    19021 tggggtaaca atatttgcac aatatggtcc cctcgcccct ctggggagag ggttagtgta
    19081 aggggaaaag cgcccaccct gccgcagcct gctccggtcg gacctagcaa ctatagctac
    19141 tcacagccag gttgattata ataaccattt atctgttcgt actgtttact aaaacgacga
    19201 atcgcctgat tttcaggcac aacaagcatc aacaataagg attaaagcta tgggttttct
    19261 ttccggtaag cgcattctgg taaccggtgt tgccagcaaa ctatccatcg cctacggtat
    19321 cgctcaggcg atgcaccgcg aaggagctga actggcattc acctaccaga acgacaaact
    19381 gaaaggccgc gtagaagaat ttgccgctca attgggttct gacatcgttc tgcagtgcga
    19441 tgttgcagaa gatgccagca tcgacaccat gttcgctgaa ctggggaaag tttggccgaa
    19501 atttgacggt ttcgtacact ctattggttt tgcacctggc gatcagctgg atggtgacta
    19561 tgttaacgcc gttacccgtg aaggcttcaa aattgcccac gacatcagct cctacagctt
    19621 cgttgcaatg gcaaaagctt gccgctccat gctgaatccg ggttctgccc tgctgaccct
    19681 ttcctacctt ggcgctgagc gcgctatccc gaactacaac gttatgggtc tggcaaaagc
    19741 gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag gtgtgcgtgt
    19801 taacgccatc tctgctggtc cgatccgtac tctggcggct tccggtatca aagacttccg
    19861 caaaatgctg gctcattgcg aagccgttac cccgattcgc cgtaccgtta ctattgaaga
    19921 tgtgggtaac tccgcggcat tcctgtgctc cgatctctct gccggtatct ccggtgaagt
    19981 ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac gaactcgaat tgaaataatc
    20041 attctgttgg taaatattgg gcggcgttct gccgcccgtt atctctgtta tacctttctg
    20101 atatttgtta tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt
    20161 cctttacgac caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta
    20221 tcatgaaacg caatccagta ctgcggagta tgacgttctg cctctggtcc cggaagccgc
    20281 aaaggtcagc gatccctttc tgctcgacgt gatccttgat gaagaaacgc tagctccctt
    20341 cctttcatgg ctggtccctg cgcgcgttct tgcagtggaa ttgttccctg accagcttac
    20401 cgtgacccgt tcacagacct tcaccgctta tgaacgcttg tctacggccc tgacggttgc
    20461 tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc
    20521 cgggcctgat tcctctaggg aaagcaatca taggttggca caaatcacgc aatatgcccg
    20581 ccaactggct agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt
    20641 cggtcttact gccagggact gtgtaatcat taaccaaatc attggtttta ttggctttca
    20701 ggcgcggacc attgcgacat ttcaggctta tctcgggcac ccggtacgct ggttacccgg
    20761 tctggaaata caaaactacg ccgacgcgtc actgtttgct gatgaatcaa tacgctggcg
    20821 aagcagctat gaggtggaaa aactacctga agagtacaca aaaagttcaa cagcagaact
    20881 ttgccaactg gccgaaacac tctctctcca ccctatttca ctttcccttc tcgaaaggtt
    20941 gttaaacagt acacgggtta atacacagcc ggataataag cttgcggcgt tgttatgcgc
    21001 gcggataaat ggcagtcctg cttgttttgc cgcctgtatg gattcatcaa atgaatataa
    21061 aaaaatcagc ccccttctgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca
    21121 ttctgttgag cacgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt
    21181 tagcaccgcc cagttcagcc ctttactcga acacgaacaa tcatcaacgc agattattaa
    21241 tctgctggta tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga
    21301 gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc
    21361 caccaaaata gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac
    21421 tgcattccca gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct
    21481 tcctggaagt cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca
    21541 tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc
    21601 cagaagaact ggttgaaccg ttcctgactc gtttcgtggg taaagttcag ggcaaaaatg
    21661 accgtctggc catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag
    21721 cccgtggcct gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc
    21781 atccgctgaa aggcgatcgt tctttctatg ctgaattgac acaatacatc acttttggtg
    21841 acgaccactt tgtaccgtgg tgggttacac ttgcgcgcca taatctggaa aaagaagcac
    21901 cagacggcgt cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgaccgcgc
    21961 tggattttgt caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta
    22021 aggcgttgcc ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga
    22081 ttgctgaagg cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc
    22141 ctggcttcaa catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg
    22201 ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcgccattg
    22261 aagataatat cgaattcttt gccgccacca tcgaatccaa agcaaagctg gtgtatgacc
    22321 aggtttctga ctggctggaa aataccggtg actggcagcc tgaaagtgaa gcaattgccg
    22381 aacaagtccg tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg
    22441 cactggtgtt taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc
    22501 tggatatcgt cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg
    22561 ccgctaacat ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg
    22621 tgcatatggg ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg
    22681 gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg
    22741 aactggacgc gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg
    22801 ctgaaattag cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct
    22861 ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta
    22921 tcaaaggcga aactgcgacg cgcccacagg atgaaatcac tgtccaaatg gccgagcgtc
    22981 gccgtctcaa ccggatggca gaacgtgatg ttggtgactg gttatacgca cgcttcctga
    23041 aagacaaagc cgggaccgac acccgtttcg cagcggaaat tgtcgatatc agccgtggcg
    23101 gcatgcgtgt gcgtttggtt gataacggcg ctatcgcctt tattccggca cctttcttac
    23161 acgctatgcg cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg
    23221 aaacggttta caaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa
    23281 cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt cacggcctat tcctcatgga
    23341 tgggccgttt atttccccgc tctaccttcg ttatatccag gcagattttt taactgactt
    23401 tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat
    23461 gcgtttagcc gccgttaatt tcatgtaagt cat
//
LOCUS       DADLTD010000045        22864 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLTD010000045 DADLTD010000000
VERSION     DADLTD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.045"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            39..968
                     /gene="aaeR"
                     /locus_tag="JN701_001418"
     CDS             39..968
                     /gene="aaeR"
                     /locus_tag="JN701_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY7363919.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1096..2541)
                     /gene="tldD"
                     /locus_tag="JN701_001419"
     CDS             complement(1096..2541)
                     /gene="tldD"
                     /locus_tag="JN701_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY7363920.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2697..6497)
                     /locus_tag="JN701_001420"
     CDS             complement(2697..6497)
                     /locus_tag="JN701_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY7363921.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6565..8034)
                     /gene="rng"
                     /locus_tag="JN701_001421"
     CDS             complement(6565..8034)
                     /gene="rng"
                     /locus_tag="JN701_001421"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY7363922.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8024..8617)
                     /gene="yhdE"
                     /locus_tag="JN701_001422"
     CDS             complement(8024..8617)
                     /gene="yhdE"
                     /locus_tag="JN701_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY7363923.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8626..9114)
                     /gene="mreD"
                     /locus_tag="JN701_001423"
     CDS             complement(8626..9114)
                     /gene="mreD"
                     /locus_tag="JN701_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY7363924.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9114..10199)
                     /gene="mreC"
                     /locus_tag="JN701_001424"
     CDS             complement(9114..10199)
                     /gene="mreC"
                     /locus_tag="JN701_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY7363925.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10265..11308)
                     /locus_tag="JN701_001425"
     CDS             complement(10265..11308)
                     /locus_tag="JN701_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY7363926.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11613..13553)
                     /gene="csrD"
                     /locus_tag="JN701_001426"
     CDS             complement(11613..13553)
                     /gene="csrD"
                     /locus_tag="JN701_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY7363927.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13705..14679
                     /locus_tag="JN701_001427"
     CDS             13705..14679
                     /locus_tag="JN701_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7363928.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15657..16127
                     /gene="accB"
                     /locus_tag="JN701_001428"
                     /gene_synonym="fabE"
     CDS             15657..16127
                     /gene="accB"
                     /locus_tag="JN701_001428"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY7363929.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16138..17487
                     /gene="accC"
                     /locus_tag="JN701_001429"
     CDS             16138..17487
                     /gene="accC"
                     /locus_tag="JN701_001429"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY7363930.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17579..18626)
                     /locus_tag="JN701_001430"
                     /pseudo
     CDS             complement(17579..18626)
                     /locus_tag="JN701_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18623..19585)
                     /locus_tag="JN701_001431"
     CDS             complement(18623..19585)
                     /locus_tag="JN701_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7363931.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19607..20596)
                     /locus_tag="JN701_001432"
                     /pseudo
     CDS             complement(19607..20596)
                     /locus_tag="JN701_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(20597..22096)
                     /locus_tag="JN701_001433"
     CDS             complement(20597..22096)
                     /locus_tag="JN701_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7363932.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22157..>22864)
                     /locus_tag="JN701_001434"
     CDS             complement(22157..>22864)
                     /locus_tag="JN701_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAY7363933.1"
                     /translation="LFANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIKADK
                     AGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQSVV
                     ARVDGFKKGLSDTPDIKIVAEQPSITRAEALTTAQNIMQANANLNGLFGFGDDAALAA
                     VIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNG
                     KPVEKMIPVQPGVYTGK"
BASE COUNT         5669 a         6192 c         5460 g         5543 t
ORIGIN      
        1 attatgtgat ctaaatcact tttaagtcag agtgaataat ggaacgacta aaacgcatgt
       61 cggtgtttgc caaagtagtt gaatttggct cttttaccgc cgccgccaga cagctacaga
      121 tgagcgtttc gtccatcagt cagacggtgt caaaactgga agatgagttg caggtaaagc
      181 tgttaaaccg tagcacacgc agcattggcc tgaccgaagc cggtagaatt tactaccagg
      241 gctgccgtcg tatgcttcat gaagtgcagg atgttcatga gcaactgtat gccttcaata
      301 acacccccat cgggacgcta cgcattggct gttcttcaac tatggcacaa aatgttctcg
      361 ccgggctgac agccaaaatg ctgaaagaat acccaggttt gagcgtcaat ctggttaccg
      421 gaattccagc ccccgacctg attgccgacg gtctggatgt ggtgatccgc gtcggcgcgt
      481 tgcaggattc cagcctgttt tcccgccgtc ttggcgcgat gccaatggtg gtgtgcgccg
      541 cgaaaagcta tctcacacaa tacggcatac cggaaaaacc cgccgattta agtagccatt
      601 catggcttga atacagcgtg cggcccgaca atgaatttga actgattgca ccggaaggga
      661 tctcgactcg cctgatcccg caagggagat ttgtgactaa tgatccgatg acgctggtgc
      721 gctggctaac ggcgggtgcc gggatcgcct acgtgccgct gatgtgggtg atcaacgaga
      781 tcaatcgtgg ggagctggag atcctgctgc cgcgttatca gtcagatcca cgcccggttt
      841 atgcgttata taccgaaaaa gataagctgc cgctgaaggt acaggtcgtg atcaactcgc
      901 tgacggatta ttttgttgag gtcggtaaat tgtttcagga gatgcacggg cgcgggaaag
      961 agaagtaatt catattgtac tgttacgttc tacaaacctg tgccaacggg ttcccctcac
     1021 cctaacccca gaggagcgag gggaccgttc gtgcacgtag aaagattaat tatccttctg
     1081 aaaataatga aattattacg cagtaccgcc aacagtcagg ttatcaactt tcagcgttgg
     1141 ctggcccacg ccaaccggca aactttgccc ttctttaccg cagacaccca cgccgttatc
     1201 cagtttcagg tcgttgccaa ccatcgaaat ctgctgcatg gtttcgatac cggaaccaat
     1261 caacgttgcg cctctcaccg gcttcgttac tttaccgttt tcaatcagat atgcttctga
     1321 agtggagaaa acgaatttgc cggaggtgat atccacctga ccgccaccaa agttcggtgc
     1381 atagataccg tactcaacgg attcaataat ttcctgcggg gtcgatttac ccggcagcat
     1441 ataggtgttg gtcatacgcg gcatgggcag atgggcgtag gattcacggc gaccgttgcc
     1501 agtcggcgtc atccccatca aacgcgcgtt gagtttatcc tgcatgtagc ctttcagaat
     1561 gccgttctca atcagcacgt tgtactggcc tggcgtacct tcgtcatcaa tcgccaccga
     1621 accacggcga tcgaccatcg tgccgtcatc aaccacggta cacagttctg aagccaccag
     1681 ctccccgacc tgcccactaa atactgaagt gccgcggcgg ttgaagtcgc cttccagacc
     1741 gtgacctacc gcttcatgca acagcacgcc cggccaacct gcgccaagta ctaccggcat
     1801 ggtgcccgct ggtgcagcaa cggcagaaag attgaccagc gccatacgca ctgcttcttt
     1861 tgcccatgca tccgcacgga cttcgccgtc aagatcggca aggaagaact cataaccaaa
     1921 acgaccgcca ccgccactgg caccgcgttc gcgtttgcca tcttcttcga ccagaacgct
     1981 cacggaaaga cgcaccagcg gacggacatc cgccgctagc gtgccgtcgg tggccgcaac
     2041 cagaattaat tcatagacac cactgaggct ggcagtcact tcctgtacgc gcttgtccgc
     2101 ttcgcgagca accttatcga cgcgacgcag gatatccagc ttctcttcac ggctcatgct
     2161 ttgcagcgga tctaccgagg tatacaacgg gctatgccct accgcgccca gcgtctgtac
     2221 tttgccatca ccactatcac ggacgatggt gcgcgccgct tgcgcactct gttccagcgc
     2281 cagcaggctg atttggtcag cgtaagcaaa tccggttttt tcaccgctga ttgcacgcac
     2341 gccaacgccc tgatcgatgt tgtaagaacc atctttaata atgcggtctt ctaaaaccca
     2401 ggattcgtga tagctcgact gaaaatagag atcgccataa tcaaggcgac gttcggccag
     2461 ttgaccgagg atcgcgaaca agtcctgatg tttcaggccg ttcgccgcta gcaattgttc
     2521 acttactagg ttaagactca tcgtttttgc tactcgttag ttactgcagt agaggatttt
     2581 ttacggctgc cggatgcggc gtgaacgccc tatccgacct acggttatgt tctggctcgt
     2641 tggcctttgg caacgattat catatgagat tggggcaatt acgcgccctc gtcaaatcat
     2701 tgcgcttttt ctttacgcgg ttggcgcaac acttcgttga tttgcggatc gtccagcgga
     2761 cccgaaatgt gatagcgcaa aatggagact ttgctccaca gcggccccag cactttactg
     2821 gctgcaaata ctgccgcgcc aacaatgggg ttaaccgcaa aagccgcagc cacgcccacc
     2881 gtcgcagaaa tctctggtgc gacaaccgct tccatattca ggtcgcgacg taccagattt
     2941 accgaccctt tcatggcgat atccgcctcc aggccatcca ccagcgtgtc gtcggtgtgc
     3001 ataacgccgt ctttaatcca cgcggtgctg cgaatggagt caaaatagaa cccttcgcca
     3061 aaagtgtctc tgaaatcaaa acgcagctta cgcatcaggg catcaacgct caataagcgc
     3121 agcaattgcc cggcatgtcc ggtattgatt tcggtaattt cgcctttacc cagttgagta
     3181 tgaatgatgc cattcaacgt cgcctcatct ggttgccagg gtgctttgcg ccagtgtaaa
     3241 tcgtaatcca cattaaatga cgactggcgt atgggcgtcg tgacaccaaa aaattctgcg
     3301 gcggcatcaa ttttctgccc gcgcagtttt cctttcagcg aggtacgttc attccccgga
     3361 ttattaaccc attcaccatc ggcagtaagc cgcgagaaac cagtatcaat cagtccattg
     3421 gtcagcgtta acgtatcgcc agaaatggtg agatcactgt caatacgacc gaatttttgc
     3481 ccccagaacc agcactctgt gcatcgtatt tgtgcgtccg gccagccacg gaagttaatg
     3541 cgctccgttg tcgggaaagg tgatgacggt gttgaatcac cacgagtttt cgccacgctc
     3601 gggttgtaat aaagatattt gatattcgcc agccacggcg cgttattacg catcgccagc
     3661 gtggcattga tttcacgccc ctgcgcctca accagggtgc catttgccgt cggttgcgaa
     3721 acaatactca ggttattcca ttgctgattt cccagtgaca acataggcgt acgtaacgtt
     3781 atgtgttgtg ggaaactcgc tgcaccaccg acactctccg cagcgccttt ctggaacagg
     3841 gccagccact cggcaccatt catcggcggc atattgagtt caacgccact ttgttccggc
     3901 aacggcggga gcgttttact gtctgccgcc caaatagcac gatcgagcgt cagcttttga
     3961 ccaagcaacc agcggctatt gaaatggtta tccgcaccag cctgtccggt taattcaaag
     4021 ctgctgagat tgccatcaac cttaacgttt accggcagtg gttcacccgc aggtttggct
     4081 aacggtgaag gtaagtgact gctcacatta ttcagatcgc cgttcagctc tacgttatag
     4141 gtcgcgccag catgataagg cagatcaatg cccactttac catcccacgc cacgctgcca
     4201 ctcaatgctt cgttcaccgc tgcaggcaga acgccggttt tcgccggttg ccagttaccg
     4261 ttgaggttta ccgcaacctg gtaggctttt gccccttctt tggtggaaaa atccacgttt
     4321 aacggctgat taaaccagct tgctgtcagt ggttcacttt gcagatcgcc attgataaag
     4381 ctgaatttac cgctcaaatt tttcagggtg ctgtcgagta gtttgataaa cagactgtta
     4441 ttacgcagcg tcacttcacc tttcgcggtt accagttcgc cgttcagcgg gatatcaaga
     4501 tgtaagcgag cattcacatc gccgtcgagc tggagttctt gcagggtcgc acccagagaa
     4561 tctttcagcg gtgtctcatc aaagtaaggg ccaacggctt tacccggacc tttaatgtca
     4621 gcgtcaatca gcagtttttc tttcgagtag tcagggatca ctgcggtgag attactcgcg
     4681 cgcacgccgc ccagattaac gtcatcggtt ttcatccata aaccgtcgtt aataaagtcc
     4741 agttcaatat caaggttagt taatgcaggc cagtccggct ggaaggcaaa cttcgcgtta
     4801 cgcagcggca ccagcacttc aaactgacct tcgttgtgtt tataggggaa gagttgcgga
     4861 ttgccaccat aaaccagcgt cgcgttatcc gcttcaccgc cctgaatcgc gccacttaag
     4921 taatcaacta ggtctttacc catcaagttt tccgggaaat agcgccaggc ttgtgaacca
     4981 tcatcggtac tgatgccagc cagaataccc agccagggtt catcgttagc aggttgcagg
     5041 taacgaaaac cgccgcgcgc atggacagct ttggctttaa cgtcaatatt acgcccatcc
     5101 aggtggaaac ctttgtcatt gttcagccag cttatagttg cctggccgtc ggcgatttcc
     5161 agtggcgcac ggaataccgt ttcgtaaggc atctttgcct gcttcatcga cgcggtaagc
     5221 aaaccatttt caacgctgcc ggaaagcgtc ccggagaagt gttccgcacc cggtaataat
     5281 ttccattgct tccaggccag atcgctccac gatgcctgaa aacgggtctt gtctgccgcc
     5341 tgaagcggga tatccagcgc cagagtgtta atcttgccgc tcggttgtgt ggagcgccaa
     5401 acatcaccca gtgcaggtga aagtttcgcg gccagcgggc gtatgccctc caggcctgcc
     5461 agctccagat tactggcgcg aatccggagt tcgtcactgc gtttattgtc tttgccgcca
     5521 acgtcctgtt ccggtatcca ggccagcgtc aatgctccgc tcggccaggg tttgccgtcc
     5581 atcgtgatcc gtgtatcggg aatagagaac tgccagcccg gattttcacg cgtaatatgc
     5641 gcggtcagat tatccaccga cagcgtatgc gtttgcttct cgcccaacca gctggcaccg
     5701 ccctgtttca gccagacgtc accgccggtt acatcgcctt tgtcgatcgt catccagcct
     5761 tcaagggaga actgtgccgt ttccagagca atattgtcct gcatccattt accgagccac
     5821 ggcttcaggt cgatgtcatc cgcctgtagc cagacgcgac cattgcttaa caacccctca
     5881 tcatcgcgca aatccatgcg cacctgcatc acgccgtgct gtccggtaag gctggagagg
     5941 cttaccaggc cttccgcacg gtgtcgacgt ggatcgttca gccaggtgag ttgtgggatc
     6001 gccagctcgg cgcgctgacc ggatggcgtc agaaaactga cttcactgtc gcggagatcg
     6061 aaatggtcaa attgacgaag aaacagatcg ctgatgtgac tggcttccag gctatcatta
     6121 ccaccaccgc tggtgatagg agtgttggtg cgaaagcgca gctgccagaa agtgaggtcg
     6181 cgaaactgcc agcgcatatg taacaagctc tgccagacat ccagcgccag agtaacgcgt
     6241 ttaaccgaaa attcgccgcc atcttttagt tctgcacgga tgtcgtgtgc ttcaagcgtc
     6301 gggccaaaat tctgccagct ggctgagagc tgactggctt ctaccggcat gccagtcgcg
     6361 gattctattt tgttgaggat ttccggacgc caggcgtcaa gatgcggtaa agcaatacgc
     6421 aggccgctaa ccagcagggc tgcgatcaca acgagcgcgg ctccagtaag cagtaaaatc
     6481 cccggcaatc gcctcacccg tcactccttg tctgctaaaa atgtgactca aaaacccttt
     6541 gccggatggc ggcccagcat ctgtttacat cattacgacg tcaaactgct cctggttata
     6601 gagcggttca atttgtactt taacctgttt gccaacgaaa atttccactt ccgccagcga
     6661 gtgtgactct tcgcctttca aggcttcagc tactgccgga gaagcataga ccaggaaacg
     6721 gtcggagtcg taagcatggt ggacacgaac aatctcgcgc atgatttcat agcataccgt
     6781 ttccacggtt ttcaccgttc cgcgaccgtg gcaggttggg cattcgttac acagtacgtg
     6841 ctcaatgctt tcgcgggtgc gtttacgcgt catctccacc agccccagcg ccgaaaaacc
     6901 attaacgctg gttttcaccc ggtctttgct caacgcctgc tccagcgagt gcagcacgcg
     6961 gcggcggtga tcttcattat tcatatcgat gaaatcaata atgataatcc cacccagatt
     7021 acgcaaccgt aactggcgag cgatagcctg cgtcgcttca atattggtat tgaaaatggt
     7081 gtcgtccaga ttgcgatgac cgacaaacgc tccggtattg atgtccacgg tggtcatcgc
     7141 ttcggtctgg tcgataatca gataaccacc ggatttcagt tctactttgc gttccagcgc
     7201 tcgctggatt tcgttttcga catcaaagag atcgaaaatc ggctggcgtc cggtgtaatg
     7261 ctccagcttg ctggtcatct cgggaatgta ctccgaggta aactccagca atgcttcgta
     7321 agtcaggcgt gagtcaacgc gaatgcggtc cagttcggcg tcggcgaaat cacgcagaac
     7381 acgctgcgcc agcgccagtt cgccgtacag ctgataacgg gtctgcgggc gttttttacg
     7441 ctccataact ttggtccaga cgcgtttcag ataagcggca tcggaggcca gttcagcctc
     7501 gccaacccct tccgctgcgg tacggatgat aaacccgccc tgctcgtcgc aatactctgc
     7561 gaccactttt ttcaggcgtt cacgttctga ttcgctttca atacgttggg aaaccccaac
     7621 gtgagaagcc cctggcataa acaccagata gcgagaaggc agcgtgatat cggtggtcag
     7681 gcgcgcacct ttagtgccaa gcggatcttt caccacctgc accatcagat cttgcccctg
     7741 acgaaccagt tccgagatgt cgcgcaccgt gaattgcttt tgttcttcac ccgccacaca
     7801 ttcggtgtgc ggcatgatat cggatgcatg aagaaacgcg gctttatcca gcccaatatc
     7861 tacaaaagcc gcctgcatac ccggaagtac acgacttaca cgacccttgt agatattgcc
     7921 tactattccg cgtcgcgcct cacgttcaat atgaatttcc tgcagaatac cgccatcaat
     7981 atacgccact cgcgtttccg aaggcgttac gtttactaac aattcagccg tcatgtttat
     8041 cccttttctc acgcagtgcg ttaaaattac ttaataattc atacgtttca accagcggta
     8101 agccgactac ggcgtgatag ctgccattta tcttcctgac aaaacagcca cccagcccct
     8161 gaataccgta tgcacctgct ttatctaacg gttcaccgct ggcgacatag cccgcgatgt
     8221 cttcgtctgt taacgttctg aaagtcacat cggtgaccac caggcaatcg agaatgtgct
     8281 ggctgtcggc caacaccact gccgtcatca cctgatgggt ctgacccgat aatttgcgca
     8341 acatctgcgc cgcatgctct gcgtcgcgcg gtttctccag cacttctcca ttcaggataa
     8401 cgatagtatc cgcacccagc accgggagat cctgcgccgt ttgcgcgaca cccgcctgtg
     8461 ctttcttgtg tgccagacgc acaacatact gctgcgcgct ctcctgcggc tgacgctgct
     8521 cctcaatgcc cgtaacaata cgttcaaagg tcacgccaag ttgcgcaagt aactcctgac
     8581 gacgcggaga accggaagct aaatacagag aagtcataga aacctttatt gcactgcaaa
     8641 ctgctgacgg actttacgca tcagcaagaa aatccacggc cagagcaccc cattgactac
     8701 actactccag aacacttccg gtctgaaaga gacgttaatc actaaaaact ctgcccagaa
     8761 aacaataata tccaccacca gcgaaagcaa catgacgact agcgcctgct gccataatgc
     8821 gaggttgcgg aaaagctggt atttcagcgc caccaggtaa gcaatgatgc tcatcgccaa
     8881 tacgcgtacg ccaagcgtcg agccgctgat cagatccagt atggcaccca tcacaaaacc
     8941 tgtgcccaca tttacgcgat ggggcaaggc caggatccaa tacaacaaga taagtaacac
     9001 ccagtttggc cggaaaacaa tcaggttatc cggccagggc atgatttgca gcaacagcgc
     9061 aatgaggaaa gagagccaga ttacccaacg tccctggcta cgatagctcg ccactattgc
     9121 cctcccggcg cacgcgcagg cggttgagca ccactttgcg gcggcgtagc cctttgtgga
     9181 gagcgattag cagcaggcgc agtagctgca tttcctgtcg tcggttgctg cggagtcggc
     9241 tgagcgatcc ccgttgtcgg ttcaggtaac tttggcccca tcgcgtctgg cgaaggcaat
     9301 acctgcggca tcatctgcat cagacgttca ttagcaacac gatgcacctc ttccggcgtc
     9361 atcgggttag cgccgttacg atccgcgccc cacagcagca gcagataacg caaacgttgc
     9421 agtcctgcag tcggacgcgc ctgaatcaca gtataagcgc gctgggtatc gagttttacg
     9481 gaagagacaa ccgcgaccgg atagccttcc gggaaacgac cgcccagacc ggaggtcacc
     9541 agcacatcac caacacgaat atccgtattc gctggcagat gctcaagctg caaatcatct
     9601 gtacaaccgt taccggctgc aattacgcgg atatcgttgc gcagcacctg gattggcagc
     9661 gcgtgggtcg catcacaaat cagcagcacg cgactggtca gtttagcgac ggccaccacc
     9721 tgaccaacaa cacctttgtc gctgatgacc ggctggcctt cataaacgcc attaacgcta
     9781 cctttatcga taacaacttg atcgctataa ggatcgttaa ccgtggagat aacctgagtc
     9841 accattttct gctcatcctg acgcagcggg gaacccagca gctcgcgcag acgcgcgttc
     9901 tcctgtttgt attgtccaag catcagcagt tcactgtttt tcagcaacag ttcctgacgt
     9961 aacgcccggt tttcaagttc tagttggtca cgcgaggcca gcgtctgcga tacgccatcc
    10021 agcaattcac gaggagcatt ggaaacaaag tagaaaggac tgacggcggt atccatataa
    10081 gtacggattt gactgaacgt ccccaggcgg ctgtcggcaa taataatgcc gagcgccacc
    10141 agcaccgcca gaataaggcg aatctgtagc gacgggccac ggctaaaaat tggcttcata
    10201 agttatgcgt attctcgtat cagaccaggc agggtaaaca gacatttccc ctgcctgcat
    10261 ccgattactc ttcgctgaac aggtcgccgc cgtgcatgtc gatcatttcc agcgctttgc
    10321 cgccaccgcg cgccacacag gtcagcgggt cttcagcaac aacgactgga atgccggttt
    10381 cttccattaa caaacggtca aggttacgca gcagtgcgcc accaccggtg agcaccatgc
    10441 cgcgctcgga gatgtcggaa gccagttccg gcgggcactg ttccagtgca accattaccg
    10501 cgctcacaat accggtcagc ggttcctgca gtgcttcgag gatttcgttg gagttcaggg
    10561 taaaaccgcg tggaacacct tctgccaggt tacggccacg aacttcgatt tcacggactt
    10621 catcgcccgg gtaagccgaa ccgatttcgt gcttgatacg ttctgcggtg gcttcaccga
    10681 tcagagaacc gtaattacga cgcacatagt tgatgatagc ttcgtcgaaa cgatcaccac
    10741 caatgcgcac ggaagaggag taaaccacgc cgttcaagga gataacagca acttcagtgg
    10801 taccaccacc gatatcaacc accatagaac cggtcgcttc agaaaccggc aggccagcac
    10861 caattgcggc agccatcggt tcttcaatca ggaagacttc acgggcacca gcgccctgcg
    10921 cggattcacg aattgcgcgg cgttcaacct gggtcgcgcc aaccggtaca caaaccagaa
    10981 cgcgcgggct tggacgcata aagttgttgc tgtgcacttg tttgatgaag tgctggagca
    11041 ttttttcagt cacgaagaag tcggcgataa cgccgtcttt cattgggcga atggcagcaa
    11101 tattgcccgg cgtacggccc agcatctgct tcgcgtcatg acctactgca gctacgcttt
    11161 tcggtgaacc ggcacgatcc tgacgaatgg ccaccacgga aggctcattc aatacgatgc
    11221 cttgtccttt tacataaatg agggtattcg cagtacccag gtcaatggac aagtcattgg
    11281 aaaacatgcc acgaaatttt ttcaacatac taagggataa tcctgaaagc tggggcggaa
    11341 aagaaaatcc gcttacttta ccaaccacac gcagcagcga caaggcgcaa aaatcatctg
    11401 ctacggtgaa aattagtgca gttcgtttcc tttgttacaa atctctgcct gagtccagaa
    11461 aggcttaatg catcagcagc attcctcgcc tgtttgcaac cgcgtaaggt cattcatctg
    11521 catatgtgct gcaacaatct ggcgagcaga caagcacact cccatgagac gcagcgcgcg
    11581 ttattctacg tgaaaacgga ttaaacggca ggttaaaccg agtatctttg tgaatatttt
    11641 ttcacgttag tatcaagtgg ctgtgaggac gcgaaaaaat ccccttgccc gcctgtaaca
    11701 ccgcgctgaa tcagggtctg ccactcgctt cgcgaacgca caccggtggc gtaaacctgg
    11761 gtgctggtcc cggagcaggc ttccaccagg ctttgaacca gcagctggtt ctccgttcgc
    11821 ttctcaatgt ttctgaccag ccccggatgg agcttgagta actcaacatt aagttctttg
    11881 atccaactgg tgcttaccag cgtcaaacca gcctggttga cggctacccg tacccctaaa
    11941 gcattcacta aacgaataac aggttgtaaa cgactgatat gttgacctac atcggcctct
    12001 gcaagttcaa taattatgcg ttttcgttgt gatttttcac attgcattaa cgtatcgcgc
    12061 agccaacgct gaaaacgcgg gcgaatcagc gactcaacgg taacctgaat cgccagattt
    12121 tcctctggcc agtaacgcaa tagtggaata agacggctga tttgcagacg gtcatactct
    12181 tccgataagc caaactgcaa gaccatcggc atatactccg ccgagctaac ctcttcatta
    12241 ccatcgaaga tgcggcacat gagttcgcga tgatgaacct gaccttcgcg agtaaccgcc
    12301 ggtttttgat aaaggcgcgg gccgccgcga ctgagcattt gctcgataag cgtacgccag
    12361 cgaacattac cgcgtccttt ttcaggcaac gagtcatcgt aaatagccca gctattgccg
    12421 ccctgcaatc ccgcattacg cgtggcagac tctgcatgtt ccattacctg ctcggtatcc
    12481 tgaccactac gccaggcgca gataccaatg tggatcatat cgtcgcgatc gagcatttta
    12541 ttgttcggca aggtatcgac ggctttgatt aactgaccgg cgatgctctc tgcctctttt
    12601 aacgtccggt gcggtaacag cgcagcaaaa tcactgcggt ggtaacgcgc cagcagtgcg
    12661 ccagggtagc gcatcataaa tgtcgacagc agattcgtca gagtgaagaa ctgttcttca
    12721 acctggctgt gcccccaagt atcgctcaac atattgaaat ccggcagacg aatcatcatc
    12781 acgatcccgt gggtacctac tttctcctga tcttccagta acgttgctaa ctgattatcg
    12841 aaaaagagtc ggttattgag gccggttttc acgtcctggg cggcataaga gcggatcagc
    12901 gtatcaagac ggctgtgttg ttcgcgtgcg ttctgaattt cacgaagcag cgtatccagc
    12961 gcactgctgg ttctgggcgg ccattcatag atggttccca acacattaga gccacgctca
    13021 ccgtttaaga tacgagtagc ccgggtttcc agcaattctt gcccggcaag ttgccgttgt
    13081 aaccagcgta ccgccaagaa gagcataaca atgataaagc taatcgcccc cgtgagcggc
    13141 gcggtggtca tcaacgaatg gaaatagttg cccatcggat cctgataaac cagacgcagc
    13201 gacatccccg gatactttat caacggaacg ctcagttcac gaaacagatc gctggagcca
    13261 accggacgat aactaccatt tctggccagg gtataaacct gtttatcacc atggagcagg
    13321 tctacacgaa cgatatctgc cgacatcatt aattcggtaa tttgtggcct taatgtgctg
    13381 aagtcattcg acacaaggtg ggtatcaatc gccgtcgcca ccgcctgaac gcgatgacta
    13441 aacttatact gaattgcgtt gtagaaactt agcgaacagc ccagcaaagt cacaaaaatt
    13501 gttaacccgg tgagcagcgt aacaaaggcc gaaaatttcg tcgttaatct catccttgtg
    13561 ttaactccga tagtgaggaa gcgggcatac tagcaaatca gatttatctc gcaatttatt
    13621 gcgcttcatc ggctttgctt ttccattagc gagtataatc ttcagaaatt attttccaat
    13681 ccatcatgca catgaggacc acttatgcag gcgttacttt tagaacagca ggacggcaaa
    13741 actctcgcat cagtacagac tctggacgaa agtcgcctgc cggagggcga tgtcatggtc
    13801 gatgttcact ggtcgagcct gaactataaa gatgcgctgg cgattaccgg taagggaaaa
    13861 atcatccgta attttccgat gattcctggg atcgattttg ccggaactgt acgcaccagc
    13921 gaagatccgc gttttcatgc cggtcaggag gtgttactca ctggctgggg cgttggtgaa
    13981 aaccactggg gtgggctggc ggagcaggcg cgagtgaaag gtgactggct ggttgccatg
    14041 ccgcaagggc tggacgcgcg taaagcaata attatcggta ctgccggttt taccgccatg
    14101 ctgtgtgtga tggcgctgga agatgccggt gttcgcccgc aggacgggga gattgtcgtg
    14161 acgggtgcca gtggtggcgt cggcagtacc gccgtggcgc tgctgcataa gttgggttat
    14221 caggtcgttg ccgtttccgg tcgcgaaagt acccatgaat atctgaaaag tttaggtgcc
    14281 agccgtattc tccctcgtga tgagtttgcc gaatcccgtc ctctggaaaa acaagtctgg
    14341 gctggggcaa ttgacactgt tggcgacaaa gtgctggcaa aagtgctggc gcaaatgaat
    14401 tacggcggct gcgtggcggc ctgtggtctg gcgggtggtt ttactctgcc aaccacggtc
    14461 atgccattta ttctgcgtaa tgtccgtttg caaggggtgg attcagtaat gacgccacca
    14521 gaacgccgcg cacaagcctg gcagcgactg gtcgccgatt taccggaatc attctatacc
    14581 caggcggcaa aagagatatc tctgtcagag gcaccgaact ttgccgaggc catcattaat
    14641 aaccagatcc agggtcgcac gctggtgaag gttaactaac catttagcaa ggaataataa
    14701 gagagggaac tcattttgac atcattattc aatcggttaa cgggaaaagc ggttagccgg
    14761 acagctttcg tcgaacacct tggtcaggaa gttgtacaac atcatccaaa ctggaaagtc
    14821 atgatttcga ctgaccacaa attgatgcgc attgatactc ctctaaacag ccattattga
    14881 tacgcctccg tcgcctgtta ggtttatggt gctttgcctg ggcgacacta cgcttaacct
    14941 gttactgatt tctggtacca tggggtgaat aatctgattt tatttgacta caaattaacc
    15001 actcgaacct atttaatgct gagaattgtc agtcggttga ttttgcgtgc tttagcattc
    15061 acatctatcc agacgatgca gtgaaaattg ggtaatcccc agcaaccgct gcgtaatgtc
    15121 gtctatcttg tcgcgatcct ggcatcccta cattatttgt ggtctgtgaa gattatctca
    15181 ttgcagcccc tcatcttcgc agggctggct ttacagcttt tcaccttacg ttataagaag
    15241 ttccgtcgat gatggcgcta atttcgtgaa ttgtgcggct tgttgcaaat tacacggtgt
    15301 tgaaggttat ttacatgtta gctgttgatt atcttccctg ataagaccag tatttagctg
    15361 ccaattgcta cgaaatcgtt ataatgtgcg acctcgtcct ccctgacgca gtttttgcgc
    15421 tgcggaaaag gtgacattgg cgcaacgaag gtatattttg ttttttgccg gaggatagca
    15481 gcagatcgct gcacaatgtc cgtcaagtct aacattgata ctctggggca aaatagaccg
    15541 gcgtcccggc ctgctggaat ttatcgctat gcatacagct gtcggggcat acgctttaca
    15601 gacggcggtg aaacgcctgt cacaatcaca ctaaacaaag agtacggaac ccactcatgg
    15661 atattcgtaa gattaaaaaa ctgatcgagc tggttgaaga atcaggcatc tccgaactgg
    15721 aaatttctga aggcgaagag tcagtacgca ttagccgtgc agctcctgcc gcaagtttcc
    15781 ctgtgatgca acaagcttac gctgcaccaa tgatgcagca gccagctcaa tctaacgcag
    15841 ccgctccggc gaccgttcct tccatggaag cgccagcagc agcggaaatc agtggtcaca
    15901 tcgtacgctc cccgatggtt ggtactttct accgcacccc aagcccggac gcaaaagcgt
    15961 tcatcgaagt gggtcagaaa gtcaacgtgg gcgataccct gtgcatcgtt gaagccatga
    16021 aaatgatgaa ccagatcgaa gcggacaaat ccggtaccgt gaaagcaatt ctggtcgaaa
    16081 gtggacaacc ggtagaattt gacgagccgc tggtcgtcat cgagtaacga ggcgaacatg
    16141 ctggataaaa ttgttattgc caaccgcggc gagattgcat tgcgtattct tcgtgcctgt
    16201 aaagaactgg gcatcaagac tgtcgctgtg cactccagcg cggatcgcga tctaaaacac
    16261 gtattactgg cagatgaaac ggtctgtatt ggccctgctc cgtcagtaaa aagttatctg
    16321 aacatcccgg caatcatcag cgccgctgaa atcaccggcg cagtagcaat ccatccgggt
    16381 tacggcttcc tctccgagaa cgccaacttt gccgagcagg ttgaacgctc cggctttatc
    16441 ttcattggcc cgaaagcaga aaccattcgc ctgatgggcg acaaagtatc cgcaatcgcg
    16501 gcgatgaaaa aagcgggcgt cccttgcgta ccgggttctg acggcccgct gggcgacgat
    16561 atggataaaa accgtgccat tgctaaacgc attggttatc cggtgattat caaagcctcc
    16621 ggcggcggtg gtggtcgcgg tatgcgcgta gtgcgcggcg acgctgaact ggcacaatcc
    16681 atctccatga cccgtgcgga agcgaaagct gctttcagca acgatatggt ttacatggag
    16741 aaatacctgg aaaatcctcg ccacgtcgag attcaggtac tggctgacgg tcagggcaac
    16801 gctatctatc tggcggaacg tgactgctcc atgcagcgcc gccaccagaa agtggtcgaa
    16861 gaagcaccag caccgggcat taccccggaa ctacgtcgct acatcggcga acgttgcgct
    16921 aaagcgtgtg ttgatatcgg ctatcgcggt gcaggtactt tcgagttcct gttcgaaaac
    16981 ggcgagttct atttcatcga aatgaacacc cgtattcagg tagaacaccc ggttacagaa
    17041 atgatcaccg gcgttgacct gatcaaagaa cagctgcgta tcgctgccgg tcaaccgctg
    17101 tcgatcaagc aagaagaagt tcacgttcgc ggccatgcgg tggaatgtcg tatcaacgcc
    17161 gaagatccga acaccttcct gccaagccca ggcaaaatca cccgtttcca cgcacctggc
    17221 ggttttggag tgcgttggga gtctcatatc tacgcgggct acaccgtacc gccgtactat
    17281 gactcaatga tcggtaagct gatttgctac ggtgaaaacc gtgacgtggc gattgctcgc
    17341 atgaagaatg cgctgcagga gctgatcatc gacggtatca aaaccaacgt tgatctgcag
    17401 atccgcatca tgaatgacga gaacttccag catggtggca ctaacatcca ctatctggag
    17461 aaaaaactcg gtcttcagga aaaataattt gtgacgcgcg gtgttttcat cgcgcttagc
    17521 aaagaacgta atcaacgccg ggcctgttaa cgctgcccgg cgtttttatt gccgttagct
    17581 aacggtagcc tgcaatttcc gctttcaacg cgctcatttc agcatcgcca tcaacaattc
    17641 ccagctcaaa gcagtatttc tttgtttccc ctggctcaat aaagggcaga cgcccctgcg
    17701 ctcgttcttt atcacgaccg tcaggaaaac tattactgac ctccagtccc ataacatact
    17761 cacccgcgcc taaatttttc cactggataa agttgggtag ctgcatggtg tcgaatttct
    17821 cgtaaattcc ccaccccagt tcggcgttca ccagagcaac tttactcatc ccctgactgt
    17881 cgctattcaa cgtgagatag taaacctgct cgtcaaaacc gggctgtggg gtacaaatct
    17941 ccagatgact ggttaaccct tccgccgcat gcggagttcg tggcgttacg cttttagccg
    18001 acagcaagat ttctggcctg tggcgataat aaaggccagc cgtaattaaa gtgataaaga
    18061 atttgcagcg gctggcgggt gaaaccatga ttagtcacct tgtcttcaat gcgcagcaca
    18121 ttttcgccat aacggcagcg aatggtcctt tccagcgtca gattttcgcc gaacagtcgc
    18181 gtttcccgta ctttgccatt gatgacaaat tcaatgccgt cgtcggtccg ttctgtgcga
    18241 taccccacct cttctgctgg agtcgcagcc aaacgcccgt gtaaccccag cgtttctcct
    18301 tcatcctcac aaggcgtgcc aatgtagcta agtccgcagg tagtcagtaa ccccgcgtaa
    18361 aagctacgta aaaatccgtt gccctgagac tcaaaaaaag cgggggcgac aatgccgttg
    18421 ggggtgataa aagaaagcgc cgtgtccttg tagctggccc atgcaatatc cagcgcgcga
    18481 tcttttacca ccgtaaatgc cagtcccgca ccagtacgga catcaaacgc ttctgtaccc
    18541 tttgctctgc cactacgtaa ctcatagtgt ttaattccgg caacctgcga gagatcgcca
    18601 atccacgacc aaatttcgtt tttcattgtg ctgcttcccg aatggacatg tcatggcgct
    18661 ggatgaacgc ttcgatttcc gcaaggcgag acgttccctc cattgggcca cgtctggtta
    18721 ccgccagcgc accgcaggca ttggcatatt gcagcgctcg atggacgtca aaccctagct
    18781 ggcggcaggc aatccacgcg ccaccaaagc agtctcccgc accggtcggg tcaacttctt
    18841 ccaccggata gctctcaaca tgaaactgct cattcgcgga gtagtaactg gcaccttgat
    18901 tgccgcgctt cacaatcacc tctttaacac cttcttcaag aaaacctgcg attgcacgct
    18961 ccggcgtcga atgtggcgac aagagcaaca cttcgccctc gctgggcata tagatatccg
    19021 ttaactcaag aacaaagtgg agggcgtcac gcatttctgg aatatcgagc atctctttac
    19081 gaatattagg gtcgaaagaa ataacaccac catttgcttt aacgatagtt accgcttttt
    19141 ttactgcgtc taccatatga aacgaaaaca gggaagagcc cataatatga aaatgggtac
    19201 agtccttcag aatattttca tcaacatgct gcgccgataa tttaccgcaa gcggcattct
    19261 taatattaaa aataaaatcg cgatcgccag agttatgata agtaacaaag gcactcccgg
    19321 tagcttccag aggtaagact gaaatccccc tgatatccac accgtcagca gccagacgat
    19381 gtatattaat atcaccaaag ccatcattcc ccacacagct aatgatgccg cacggaacgc
    19441 caagacgtgt cacctggtcg ataaaaatag cgggcgcacc actgggataa gggccgttcc
    19501 agatacctgg ttgatcaaat ggctggccga ttttactcgc cataatttca accagaattt
    19561 ctccaattgt gaagacctta ctcatatgat taattccttg aaatgattag ctgcgttttc
    19621 ttttatttct tacgtcaaaa taaacggcta caagaatgac taatccttta accaccatct
    19681 ggtaaaaata gggaacattc attaagttca tgccattatt aataacgccg atagttaatg
    19741 cgccgatcac cgtccccacc acggttccat agccgccgtt caggctggtg cctcccagta
    19801 cagccgcagc aatagcatcc agttcgtagc tcaacgcgga attcggttac ccggaataga
    19861 ggcgtgaggc gaggatcatc ccgctgatac ctgccagcac ggctgtaatc ataaagactt
    19921 taattttcaa gcgggtgaca tttatccctg aataaattgc cgcttcttta ttaccaccag
    19981 tgatataaat ttttcgtcca aaggttgttt tagtaagaat gaagtggtta atcagcaata
    20041 agatgattaa tatccagatt ggaataggta tatagagaaa ttctccattc cccagtgcca
    20101 gaaaggcatc atctttaatc atgatgggca taccatcggt cactatataa gcgataccac
    20161 ggaagatccc catagtagca acggtgacaa taaacgacgg gatccccgct atagccgtca
    20221 agccaccatt tatcatcccc agagtcagtg ctgcaataat ggataatggg accaataaaa
    20281 taaatggaat tccccatgaa gccccaagcg cagcaaatgt gccaatcaat gcaatggttg
    20341 aaccgaccga taaatcaata tcgccaagca aaagtacata agtcattcca taggcggtaa
    20401 ttgcaattat cgaaacctga agaataatat tagtaatgtt gttggcggta aaaaaactgt
    20461 cattaataaa actgaacaga atgattaaca ccaccattcc actgaggata ccgccatatt
    20521 gcctgaagac ttttttaaaa ttatttgcaa tgggaatttc cgaaggtttc ggactggcaa
    20581 gagttttctc tgtcatttaa cgtactcctg ttgcagcggt cattacctgt gtctgcgtta
    20641 aattttcaca tgaataaata tcggcaatcg ttttattacg cattaccacc agacgatcgc
    20701 taagtgccat gacttctggc agttcagagg acaccagaat aattgctgtc cctgccgcag
    20761 ccagttgacg tatcacgtta tagatttcaa attttgcgcc gacatccact ccgcgcgttg
    20821 gctcatcgag caataaaata cgcggagttt tctccagcca cttcgataac acgattttct
    20881 gttgattacc gccgctcagc gctcccactg cctgatgtac agaactcact ttagtgttca
    20941 ttctggcaat tgattgcagc gtcttcgcct cttcttgtcg gtgattgatg agtcccatcc
    21001 gcgaagcgtt atattgaaac gccacatggt gcgtgttcat attgacgtcg tgcatcagca
    21061 ctaacccttc ttccttgcgg ttttcggtca caaaagcaat gccatgatca atagcctgtt
    21121 taggattagt gatcctgact tccttaccat caatcaacac agtcccctga tacgaaacga
    21181 ggccaaagag cgccttcatc acatcggaac gcccggcccc caccaggcca aaaaatccga
    21241 ggacctcccc cggacgcaca gcgaaactga tctctttaaa aagcgaagga tgggaaagat
    21301 tttttacttc cagttttgca ggaacatttt catccggtat ttccccaaga cgtgctggcc
    21361 aaatattttt catttcccga ccaaccattt gggcaatcaa atgctggata tcactgtttt
    21421 tcttttcatg cgtggcgata tactcaccgt cgcggatcac cgaaatatca tctgaaatcc
    21481 gcataatttc atccatgcga tgagagatat aaataaccgc acagcctcgc tgctttaatt
    21541 tttcaataat gttaaacaga atctccgctt cattatcact tagcgaagat gtcggttcat
    21601 ccataatcac gacatccgct gcatagctta aggctttagc aatttctacc agttgcctat
    21661 gtgccatcga catattgccg accatttcta gtgggtctag tggtatgccc cgttccgcga
    21721 ggagattctg gcaatctgcg agcatttttt tatcgttaat aatgccaaaa cgtcgcggtt
    21781 cattattggc ataaatattt tccgccacgg tcatattatt actcaggctc aattcctgaa
    21841 aaataatcgc aattcctaac tggcgtgaat gctgcggatt attaatggtg atagtttcac
    21901 cacgtagtgt tatcgttccc tgggttggcg gataaacacc ggccagtatt ttcattaatg
    21961 tcgatttacc ggcaccattc tcgcctaata aagtatgcac ccgcccttta cgtaaagaga
    22021 gggtaatatc ttttaatgct tgaacatgac caaatgtctt cgcaatatgc gaaacatcaa
    22081 gaataacatc cgtcataatg acctcggtgt cacaacaaaa cgccaataac gttttgttgt
    22141 gaggtgaaga tgtttactat ttacctgtat aaacgccggg ttgtaccggg atcatttttt
    22201 ctaccggttt accgttgagg tgatcaacag cggcatcaat ggctttagca cccatttgat
    22261 cgggatattg ccgaatcacg gccgcaaacg ttttttcact gtcaacggca tcacgcgctt
    22321 ctttcatgcc atcaaaacca atgattttga tgttgtcgtt atgcgcagat tttgctgcga
    22381 taaccgctgc cagcgccgca tcatcaccaa agccaaacag accattcagg ttggcatttg
    22441 cctgcataat gttttgcgca gtcgttaacg cttcagcccg cgtaatacta ggctgttcag
    22501 caacaatttt aatatccggt gtatcgctta accctttctt aaaaccatca acgcgcgcaa
    22561 ccaccgattg tagtgccgga taagaaataa tggctacagt acctttacca ttcaaaagtt
    22621 tgcccattaa cttgcctgct tcaacgcctc ccgcatagtt atcggtcgca acatgcgata
    22681 cgaccggaac gccttccgca gcgacgtcga cggtgataac gggaataccg gccttatcgg
    22741 ctttaattat cgccgcctgg acgcctttcg aatcgaccgg agagataata atggcatcca
    22801 cttttttagt aataaaatcc tcaacatcag aaagttgttt gtttaaatcc tgattggcaa
    22861 atag
//
LOCUS       DADLTD010000046        22857 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLTD010000046 DADLTD010000000
VERSION     DADLTD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.046"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..353)
                     /locus_tag="JN701_001435"
     CDS             complement(<1..353)
                     /locus_tag="JN701_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY7363934.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGL"
     gene            complement(350..1009)
                     /locus_tag="JN701_001436"
     CDS             complement(350..1009)
                     /locus_tag="JN701_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7363935.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(1230..1289)
                     /gene="ibsA"
                     /locus_tag="JN701_001437"
     CDS             complement(1230..1289)
                     /gene="ibsA"
                     /locus_tag="JN701_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAY7363936.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(1562..1618)
                     /locus_tag="JN701_001438"
     CDS             complement(1562..1618)
                     /locus_tag="JN701_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7363937.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(1890..1946)
                     /locus_tag="JN701_001439"
     CDS             complement(1890..1946)
                     /locus_tag="JN701_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7363938.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            2225..3472
                     /gene="mdtA"
                     /locus_tag="JN701_001440"
     CDS             2225..3472
                     /gene="mdtA"
                     /locus_tag="JN701_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAY7363939.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            3472..6594
                     /gene="mdtB"
                     /locus_tag="JN701_001441"
     CDS             3472..6594
                     /gene="mdtB"
                     /locus_tag="JN701_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY7363940.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            6595..9672
                     /gene="mdtC"
                     /locus_tag="JN701_001442"
     CDS             6595..9672
                     /gene="mdtC"
                     /locus_tag="JN701_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY7363941.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            9673..11089
                     /locus_tag="JN701_001443"
                     /pseudo
     CDS             9673..11089
                     /locus_tag="JN701_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11086..12489
                     /gene="baeS"
                     /locus_tag="JN701_001444"
     CDS             11086..12489
                     /gene="baeS"
                     /locus_tag="JN701_001444"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY7363942.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPSVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            12486..13208
                     /gene="baeR"
                     /locus_tag="JN701_001445"
     CDS             12486..13208
                     /gene="baeR"
                     /locus_tag="JN701_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY7363943.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            13399..13731
                     /locus_tag="JN701_001446"
     CDS             13399..13731
                     /locus_tag="JN701_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY7363944.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            13854..15254
                     /locus_tag="JN701_001447"
     CDS             13854..15254
                     /locus_tag="JN701_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HAY7363945.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            15575..16936
                     /locus_tag="JN701_001448"
     CDS             15575..16936
                     /locus_tag="JN701_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY7363946.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(17266..17583)
                     /locus_tag="JN701_001449"
     CDS             complement(17266..17583)
                     /locus_tag="JN701_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363947.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            17997..18901
                     /gene="yegS"
                     /locus_tag="JN701_001450"
                     /pseudo
     CDS             17997..18901
                     /gene="yegS"
                     /locus_tag="JN701_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(18983..19762)
                     /locus_tag="JN701_001451"
     CDS             complement(18983..19762)
                     /locus_tag="JN701_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7363948.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19862..20902)
                     /gene="gatD"
                     /locus_tag="JN701_001452"
     CDS             complement(19862..20902)
                     /gene="gatD"
                     /locus_tag="JN701_001452"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7363949.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(20950..22305)
                     /locus_tag="JN701_001453"
     CDS             complement(20950..22305)
                     /locus_tag="JN701_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY7363950.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(22309..22593)
                     /gene="gatB"
                     /locus_tag="JN701_001454"
     CDS             complement(22309..22593)
                     /gene="gatB"
                     /locus_tag="JN701_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY7363951.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(22624..>22857)
                     /locus_tag="JN701_001455"
     CDS             complement(22624..>22857)
                     /locus_tag="JN701_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY7363952.1"
                     /translation="YLLRPTNKVHFQQADDDNDVEVSLVIALIVENPQQQLKLLRCLF
                     GKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
BASE COUNT         5541 a         6007 c         5748 g         5561 t
ORIGIN      
        1 atcaatccca gaaacgtgca ggcaaaatcg cgaactgcta atgctttggc ttctgcctcg
       61 gcaaaaagtc gttgacgaat agtcagtacc atatttgtgg cgaggacatc attaagcccc
      121 aattcaccgt cctgtacttt ttgcaccata aacgccatcg cttcattgac ggcgagcgtc
      181 gcgccttctc ctccctgcga aacgctacct gcgccatcag caaggaacat caccagtaat
      241 ggttgctggt cgtttaacca gacaacctgc atctggcagg catcctgaca aggcaaatca
      301 gcactaatgt gcgatgttcc aacagcggat gcataaacca gacgccagct cacactgacg
      361 tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac
      421 gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccctgc agtggcaaag
      481 gctgacgaac gctgatttgt gccagagtct tcatatccgc cccctgaaca ccgatggaaa
      541 agaaggcaaa cttcttatct tcttccccct gaaacacttt gttggcggca gcctgccact
      601 catcggttgg agcgccatcg gtgatcagga aaatccacgg gcgataatac gaaataccat
      661 ttgcgcggta ctcacgtttc ctctcttcaa ccatatctag ggctttggta atggcggccc
      721 ccataggcgt atccccctgg gcaaacagga tgggcgggaa aaagttagcg gcgctggtaa
      781 agggttgttc cacatgcacc gggccgaacg tcacaatgcc gagttccact ctttttaaag
      841 ccagcggatc agcaagcagt tcatcacgaa aggtaaccaa tccggcgtta agttcattaa
      901 taggtctgcc gctcatcgag ccagaaacat ccagcagcaa aatgcagggg caacgtggtt
      961 ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata agaaatcctt
     1021 tcttagagaa ttatcgaggt ccggtatcat accgattgca ataatgaaat aagagcaaac
     1081 gaagaaactt tcagaattag gcccgtcggc gcagaaaatt ttgctattcg cctcgcaaga
     1141 acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag tgagggttag
     1201 ggagaggttt ccccctcccc ctggtgttct tagtaagcct ggaagctaat cactaagagt
     1261 atcaccagta tgatgacgtg cttcatcata accctttcct tattaaaagc cctcttctcc
     1321 gggagaggct ttcccgtttc agcgtcccgc tgaaatcatc ggcttacctc ctttcgccct
     1381 gaatgcagtc tatcgctaac gcgtgaataa aatagtttcc tctgtgatta ttgcgtaacg
     1441 actcgcaaag aatatttgct ggcaggatcg cagactacaa agcctacgga ttgacaatct
     1501 tatcgtgaag gcatactttc aggagtgagg gtagagcggg gtttcccccg ccctggtagt
     1561 cttagtaagc ggggaagctt atgactaaga gcaccacgat gatgagtagc ttcatcatga
     1621 ccctttcctt atttaaggcc ccttcctcgg gaggggcttt cccgtttcag cgtcccgctg
     1681 aaatcgtcgg cttacctcct ttcgccctgt atgcagtcta tcgctgactt gcggataaaa
     1741 tagtttccag tgtgattatt gcgtaacgtc tcgcaaagaa tatttgctgg caggatcgca
     1801 gactgcaaag cctgcagatt gacaatctta tcgtgaaggc atactttcag gagtgagggt
     1861 agagcggggt ttcccccgcc ctggtagtct tagtaagctg gccagctaat gactaagagc
     1921 accacgatga tgagtagctt catcatgacc ctttccttat ttatggcccc ttcctcggga
     1981 ggggctttcc cgtttcagcg tcccgctgaa atcggcggct tacctccttt cgccatgcaa
     2041 gcagtctatc cctaacgcgt agataaaata gtttcctgtg ttattactgg atgcgtgctc
     2101 gcaaatgtgc ccgtcattca gacaattccc gacagtgttt cataattcct ccatttttct
     2161 cccttattgg ctggctacac tagtatcatt ccgcgaaacg tttcaggaag agaaactctt
     2221 aacgatgaaa ggcagttata aatcccgttg ggtaatcgta atcgtggtgg ttatcgccgc
     2281 catcgccgca ttctggttct ggcaaggccg caatgactcc cggagtgcag ccccaggggc
     2341 gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt ggtatgcgtt ccggcccatt
     2401 agccccggtt caggcggcga ccgccgtaga acaggcagtt ccgcgttacc tcaccgggct
     2461 tggcaccatt atcgccgcta atactgttac ggtgcgcagc cgcgtggacg gtcaactgat
     2521 ggcgttacat ttccaggaag gccagcaggt caaagcaggc gatttactgg cagaaattga
     2581 ccccagccag ttcaaagttg cattagcaca aacccagggc caactggcaa aagataaagc
     2641 cacgcttgct aacgcccgcc gtgatctggc gcgttatcag caactggcaa aaaccaatct
     2701 cgtctcccgt caggagctgg atgcccaaca ggcgctggtc agtgaaaccg aaggcaccat
     2761 taaggctgat gaagcaagcg tcgccagcgc gcagttgcaa ctcgactgga gccgcatcac
     2821 cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat gttggtaacc aaatctccag
     2881 tggtgatacc accggaattg tggtgatcac ccagacgcat cctatcgatt tagtctttac
     2941 cctgccggaa agcgatatcg ctactgtagt gcaggcgcaa aaagccggaa aaccgctggt
     3001 ggtagaagcc tgggatcgca ccaattcgaa gaaattaagt gaaggcacgc tgttaagcct
     3061 cgataaccaa atcgatgcca caaccggtat gattaaagtg aaagcacgct ttaataatca
     3121 gaatgatgtg ctgtttccca atcagtttgt taacgcgcgc atgttagtcg acaccgaaca
     3181 aaacgccgta gtgatcccaa cagccgccct gcaaatgggc aatgaaggcc attttgtctg
     3241 ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg acgccgggca ttcaggacag
     3301 tcagaaagtg gtgatccgtg caggtatttc tgcgggcgat cgcgtggtga cagacggcat
     3361 tgatcgcctg accgaagggg cgaaagtgga agtggtggaa gcccagagcg ccaccactcc
     3421 ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg
     3481 ttacccccga gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc
     3541 acgctgctga tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt
     3601 tcggcgctgc cggaagtgga ctatccgacc attcaggtgg tcacgctcta cccaggtgcc
     3661 agcccggatg tcatgacctc tgccgttacc gcgccgctcg aacgccagtt cgggcagatg
     3721 tctggcctga aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat cactttgcag
     3781 ttccagctaa cattaccgct cgatgtcgcc gagcaggaag tgcaggccgc gattaacgct
     3841 gcgaccaact tgttgccgag cgatctgcct aacccgccgg tttacagcaa agtgaacccg
     3901 gcagatccgc cgatcatgac gctcgccgtc acctcaaccg ccatgccgat gacgcaagtg
     3961 gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg
     4021 gtgacgcttt ccggcggtca gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt
     4081 gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac
     4141 tcggcaaaag gtagcctcga cggcccttcc cgtgcggtca cgctttccgc gaacgaccag
     4201 atgcaatccg ccgaagagta tcgccagcta atcatcgcct accagaacgg cgcgccaatt
     4261 cgtctgggcg atgtcgcaac tgtagagcaa ggtgcagaaa acagctggct cggcgcgtgg
     4321 gcgaacaaag aacaggccat tgtgatgaat gttcagcgcc agcccggtgc taacattatc
     4381 tccaccgccg acagcattcg gcagatgctg ccacagctca ctgagagtct gccgaaatcg
     4441 gtgaaggtga cagtgctttc cgatcgcacc accaatatcc gcgcatccgt cgatgatact
     4501 cagtttgaat tgatgatggc tatcgcgctg gtagtcatga ttatctacct gtttttgcgc
     4561 aatattccgg cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat cggcactttc
     4621 gcggttatgg tgtttctcga tttttcaatc aataacctga cactgatggc gttaactatc
     4681 gccaccggat tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc ccgctatatc
     4741 gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat cggctttacc
     4801 attatctcgc tgatcttctc actgattgcg gtgttgatcc cactgctgtt tatgggcgat
     4861 atcgtcgggc gactgttccg cgaatttgct attaccctgg cggtagcgat tttgatctca
     4921 gcggtggtgt cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg
     4981 ttgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc
     5041 gcctatggtc gtggactggc gaaagtgctg aatcatccgt ggctgacctt aagcgtggca
     5101 ctcagcacgc tgctgcttag cgtgctgctg tgggtgttca ttccgaaagg tttcttcccg
     5161 gtacaggaca atggcattat tcagggcact ttgcaggcac cgcaatccag ctcctttgcc
     5221 aatatggccc agcgacaacg ccaggtcgcg gacgtgattt tgcaggatcc ggcagtgcaa
     5281 agcctgacct catttgttgg cgttgatggc actaacccgt cgctgaatag tgcacgttta
     5341 caaatcaacc tcaaaccgtt ggatgaacgt gatgatcggg tgcaaaaagt catcgcccgt
     5401 ctgcaaacgg cggtagataa agtgccgggc gtcgatctct tcctgcaacc aacgcaggat
     5461 ctgactattg atactcaggt cagccgcacc cagtaccagt ttaccttgca ggccacctca
     5521 ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa aactccagca actgccacag
     5581 ctttctgatg tctccagcga ctggcaggac aaagggctgg tggcgtatgt caatgttgat
     5641 cgcgacagcg ccagccgtct ggggatcagc atggcggatg tcgataatgc cctgtacagc
     5701 gcgtttggtc agcgactgat ttccactatt tatactcagg ctaaccagta tcgcgtagtg
     5761 ctggagcaca acaccgaaat taccccgggc ctcgcggcgc tggataccat tcgcctgacc
     5821 agtagcgacg gcggcgtggt gccgttaagc tcaattgcca aagttgaaca gcgttttgcg
     5881 ccgctttcca tcaaccatct ggatcagttc ccggtaacga ccatctcctt taacgtgccg
     5941 gataactatt cgctgggcga tgcggtgcag gcgattatgg acaccgaaaa gacgctgaat
     6001 ctgccggtgg atatcaccac gcaattccag ggcagcaccc tcgccttcca gtcggcgctg
     6061 ggcagcactg tctggctgat tgtcgcggcg gtggtggcga tgtatatcgt gctcggcatt
     6121 ctgtacgaga gctttattca cccgatcacc attctctcga cgctacccac cgcaggggtc
     6181 ggcgcgctgc tggcattaat gattgccggt agcgaactgg atgtgattgc gattatcggc
     6241 attattttgc tgatcggtat tgtgaagaag aacgccatca tgatgatcga cttcgcgctg
     6301 gctgctgagc gcgagcaagg catgtcgccg cgcgatgcaa tctaccaggc ttgtctgttg
     6361 cgttttcgtc cgatcctgat gaccaccctg gcggctctgc ttggcgcgct gccgctgatg
     6421 ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag gtatcggcat ggtcggcggt
     6481 ctgattgtca gccaggtgct gacgctgttc accacgccgg tgatttatct gctgttcgac
     6541 cgcctggcat tgtggaccaa aagtcgcttt gctcgccatg aagaggaagc gtaagtgaag
     6601 ttttttgccc tcttcattta ccgtccggtg gcgacgattt tactgtcggt tgccattacc
     6661 ctgtgcggca tactgggctt ccgtatgctg ccggtcgccc cgctgccgca ggtcgatttt
     6721 ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac cagaaacaat ggcgtcttcc
     6781 gttgccacgc cgctggagcg ctcacttggg cgcattgccg gagtcagtga aatgacctcc
     6841 agcagttcgc tcggcagcac gcgtattatt ttgcagtttg attttgaccg ggatatcaac
     6901 ggcgcggcgc gcgatgtgca ggcggcgatc aacgctgcac aaagtttgct gcccagcggg
     6961 atgcccagcc gtccgaccta tcgcaaagct aacccgtcgg atgcgccaat tatgatcctc
     7021 acgctgacat ccgatactta ttcgcagggt aaactgtacg atttcgcctc gacgcagctg
     7081 gctccgacga tttcgcaaat cgacggtgtt ggtgatgtcg atgtcggcgg cagctcactg
     7141 cccgccgtac gcgtcgggct gaatccgcag gcgctgttta atcagggcgt ctcgctggac
     7201 gacgtacgca ctgccatcag caatgccaac gtgcgtaaac cgcagggggc gctggaagat
     7261 ggcactcacc gctggcagat ccagaccaat gatgagctaa aaaccgccgc cgaatatcag
     7321 ccgctgatta ttcattacaa caacggcggc gcggttcgtc tgggcgatgt ggcgacagtg
     7381 accgactctg tacaggatgt acgtaacgcc gggatgacca atgccaaacc ggctattttg
     7441 ctgatgatcc gcaagctgcc ggaagccaat attatccaga cggttgacag catccgggca
     7501 aaattaccgg agttgcagga aaccattccg gcggcgattg atctgcaaat tgctcaggat
     7561 cgctccccca ccattcgcgc ctcgctggaa gaagtcgagc aaacgctgat tatctcggtg
     7621 gcgctggtga ttctggtggt gtttttattc ctgcgctcgg gtcgcgcaac tattattccc
     7681 gccgttgcgg tgccggtttc gctgattggt acgtttgcgg cgatgtacct gtgcggtttc
     7741 agtctcaata atctttcgtt aatggcgctc accatcgcta ctggtttcgt ggtggatgac
     7801 gccatcgtgg tgctggaaaa cattgcccgt catctggaag cgggaatgaa accgttgcaa
     7861 gccgcactgc aaggtactcg cgaagtcggt tttacggtgc tgtcgatgag tctgtcactg
     7921 gtggcggtgt tcctgccgct gctgttgatg ggcggattgc cgggccgact gttacgcgaa
     7981 tttgccgtga cgctttctgt cgccattggt atatcgttgc tggtttctct gacattaacg
     8041 ccaatgatgt gtggctggat gctgaaagcc agcaagccgc gcgagcaaaa gcgactgcgt
     8101 ggttttggtc gcatgttggt agccctgcaa caaggctacg gcaagtcact gaaatgggtg
     8161 ctcaatcata cccgtctggt gggcgtggtg ctgcttggca ccattgcgct gaatatctgg
     8221 ctgtatatct cgatcccgaa aaccttcttc ccggagcagg acactggcgt gttgatgggc
     8281 gggattcagg cggatcagag tatttcgttt caggcgatgc gcggtaagtt gcaggatttc
     8341 atgaaaatta tccgtgacga tccggcagtg gataatgtca ccggctttac aggcggttcg
     8401 cgagtgaaca gcgggatgat gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg
     8461 gcgcagcaaa ttatcgaccg tctgcgcgta aaactggcga aagaaccggg ggcgaatctg
     8521 ttcctgatgg cggtacagga tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag
     8581 tacacgttgt tatccgacga cctggcggca ctgcgagaat gggagccgaa aatccgcaaa
     8641 aaactggcga cgttgccgga actggcggac gtgaactccg atcagcagga taacggcgcg
     8701 gagatgaatc tggtttacga ccgcgacacc atggcacggc tgggaatcga cgtacaagcc
     8761 gccaacagtc tgttaaataa cgccttcggt cagcggcaaa tctcgaccat ttaccagccg
     8821 atgaaccagt ataaagtggt gatggaagtg gatccgcgct atacccagga catcagtgcg
     8881 ctggaaaaaa tgttcgttat caataacgaa ggcaaagcga tcccgctgtc gtatttcgct
     8941 aaatggcaac cggcgaatgc cccactatcg gtgaatcatc agggattatc ggcggcctcg
     9001 accatttcgt ttaacctgcc gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat
     9061 cgcgcaatga cccagcttgg ggtgccttcg acggtgcgcg gcagttttgc cggcacggcg
     9121 caggtgttcc aggagacgat gaactcgcag gtgatcctga ttatcgccgc catcgccacg
     9181 gtgtatatcg tgctgggtat cctttacgag agttacgtac atccgctgac gattctctcc
     9241 accctgccct cggcgggcgt tggagcgctg ttggcgctgg agctgttcaa tgccccgttc
     9301 agcctaatcg ccctgatagg gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt
     9361 atgatggtcg attttgcgct tgaagcccaa cggcacggta acctgacgcc gcaggaagct
     9421 attttccagg cctgtctgct gcgttttcgc ccgattatga tgaccaccct ggcggcgctg
     9481 tttggtgcgc tgccgctggt attgtcgggc ggcgacggct cggagctgcg gcaacccctg
     9541 gggatcacca ttgtcggcgg actggtaatg agccagctcc ttacgctgta taccacgccg
     9601 gtggtgtatc tctttttcga ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg
     9661 gtaaccgagt aaatgacaga tcttcccgac agcacccgtt ggcaattgtg gattgtggct
     9721 ttcggcttct ttatgcagtc gctggacacc accatcgtaa acaccgccct tccctcaatg
     9781 gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg tcattgtctc ttatgtgctg
     9841 accgtggcgg tgatgctgcc cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat
     9901 attttcttta ccgccatcgt gctgtttact ctcggttcac tgttttgcgc gctttccggc
     9961 acgctgaacg aactgttgct ggcacgcgcg ttacagggcg ttggcggcgc gatgatggtg
    10021 ccggtcggca gattgacggt gatgaaaatc gtaccgcgcg agcaatatat ggctgcgatg
    10081 acctttgtca cgttacccgg tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg
    10141 ctggtggagt acgcgtcgtg gcactggatc tttttgatca acattccggt ggggattatc
    10201 ggtgcgatcg ccacattgct gttaatgccg aactacacca tgcagacgtg gcgctttgat
    10261 ctctccggat ttttattgct ggcggttggc atggcggtat taacccctgg cgctggacgg
    10321 cagtaaaggt acaggtttat cgccgctggc gattgctggc ctggtcgcag ttggcgtggt
    10381 ggcactggtg ctttatctgc tgcacgccag aaataacaac cgtgccctgt tcagtctgaa
    10441 actgttccgt actcgtacct tttcgctggg cctggcgggg agctttgccg gacgtattgg
    10501 cagtggcatg ttgcccttta tgacaccggt tttcctgcaa attggcctcg gtttctcgcc
    10561 gtttcatgcc ggactgatga tgatcccgat ggtgcttggc agcatgggaa tgaagcgaat
    10621 tgtggtacag gtggtgaatc gctttggtta tcgtcgggta ctggtagcga ccacgctggg
    10681 tctgtcgctg gtcaccctgt tgtttatgac taccgccctg ctgggctggt actacgtttt
    10741 gccgttcgtc ctgtttttac aagggatggt caactcgacg cgtttctcct ccatgaacac
    10801 cctgacgctg aaagatctcc cggacaatct ggcgagcagc ggcaacagcc tgctgtcgat
    10861 gattatgcaa ttgtcgatga gtatcggcgt cactatcgcc gggctgttgc tgggactttt
    10921 tggttcacag catgtcagcg tcgacagcgg caccacacaa accgtcttta tgtacacctg
    10981 gcttagcatg gcgttgatca tcgcccttcc ggcgttcatc tttgccagag tgccgaacga
    11041 tacgcatcaa aatgtagcta tttcgcggcg aaaaaggagc gcgcaatgaa gttctggcga
    11101 cccggtatta ccggcaaact gtttctggcg attttcgcca cctgcattgt cttgctgatc
    11161 agtatgcact gggcggtgcg tatcagtttt gagcgtggct ttattgatta catcaagcat
    11221 ggtaatgaac agcgattaca actgttaagt gatgcgcttg gcgagcagta tgcgcagcat
    11281 ggcaactggc gcttcctgcg caacaatgat cgctttgtct ttcagatcct gcgttcattt
    11341 gaacacgata attcggaaga taaacccggc ccgggtatgc caccgcacgg ctggcgtacc
    11401 cagttctggg tggttgatca aaacaacaaa gtgctggttg gtccgcgagc gccgattcca
    11461 cctgacggta cacggcgacc cattctggtc aacggtgcgg aagttggcgc ggtgatcgcc
    11521 tcccccgttg agcggttaac gcgcaatact gatatcaatt tcgataaaca acagcggcaa
    11581 accagctggt tgattgtcgc cctggcaacg ttactcgcgg cacttgccac ttttctgctg
    11641 gcgcgcggtt tactggcacc ggtaaaacga cttgtcgatg gcacgcacaa actggcggcg
    11701 ggcgatttca ctacccgcgt aacgcccacc agtgaagatg aactgggcaa actggcgcaa
    11761 gacttcaacc agcttgccag cacactggag aaaaaccagc aaatgcggcg cgattttatg
    11821 gccgatattt ctcacgaact gcgtacgcca ttagcggtgc tgcgcggtga actggaagcc
    11881 attcaggatg gcgtgcgtaa attcacgccg gagacggtgg cgtctttaca ggcggaggtc
    11941 ggtacactga ccaaactggt tgacgatctc catcagttgt cgatgtctga tgaaggcgct
    12001 ctcgcctatc aaaaagcacc ggtagatttg atcccactgc tggaagtggc gggcggcgca
    12061 tttcgcgaac gattcgccag tcgtggcctg aaactgcaat tttccctgcc agacagtatt
    12121 accgtatttg gcgatcgcga ccgtttaatg cagttattca ataatttact tgaaaacagc
    12181 ctgcgctata ctgacagcgg cggcagcctg aaaatctctg ccgagcagca cgacaaaacg
    12241 gtgcgcctga cctttgccga cagtgcgcca agtgtcagtg acgagcagct acaaaaatta
    12301 tttgaacgtt tttatcgcac cgaaggttcc cgcaaccgcg ccagcggtgg ttccgggctg
    12361 gggctggcga tttgcctgaa cattgttgaa gcacataatg gccgcattat tgctgcccat
    12421 tcgccttttg gcggggtaag cattacagta gagttaccgc tggaacggga tttacagaga
    12481 gaagtatgac cgagttacca atcgacgaaa acacaccacg tattttgatc gtggaagatg
    12541 aaccgaagct ggggcagttg ctcattgatt atctgcgtgc tgcgagctat gcgccgacgc
    12601 ttatcagcca cggcgatcag gtactgccgt atgtgcgcca gacaccaccg gatctgatcc
    12661 tgttggatct gatgctccct ggcaccgatg gcttgacgct gtgccgggaa attcgtcgtt
    12721 tttctgacat tcctatcgtg atggtgacgg caaaaatcga agagatcgac cgcctgctcg
    12781 gactggagat tggcgcagat gactacatct gcaagccgta cagcccacgg gaagtggtag
    12841 cgcgcgtcaa aaccattttg cgccgttgca aaccgcagcg cgaattgcag caacaggatg
    12901 ccgaaagccc gttgattatc gacgaaggtc gttttcaggc ttcatggcgc ggtaaaatgc
    12961 ttgacctgac acctgcggaa tttcgtctgt tgaaaacgct ctctcatgaa ccaggaaaag
    13021 tgttctcccg cgagcaattg ctcaatcatc tttatgacga ctaccgcgta gtaaccgacc
    13081 gcaccattga cagccacatt aaaaacctgc gccgcaagct ggaatctctc gacgccgaac
    13141 agtcatttat ccgcgccgtt tacggcgttg gttaccgctg ggaagccgac gcctgccgca
    13201 tcgtttagtt ttagcgacat gactttgtta gccggagatg gcgttcagac cagatccggc
    13261 aacattatcc cacgcatggt cagcaaactg acattatctc tccgccctgg caggcatcag
    13321 aaaatcgctc atactttaat cggtaaacag cacctttaga tgctgttttc gatacgcaat
    13381 ttcaatcaag gagtcattat ggctggttgg tttgaactca gtaagagcag tgataatcag
    13441 ttccggtttg tgctaaaagc gggcaatggt gagactatcc tcaccagcga gctgtataca
    13501 tcaaaagcct ctgcggaaaa gggcatcgcg tcagtgcgta gcaacagccc gcaagaagaa
    13561 cgctatgaga aaaaaacggc aagtaacggc aaattctatt tcaatctgaa agccgccaat
    13621 catcaaatta tcggctccag ccagatgtac gccaccgcgc aatctcgtga aaccggaatt
    13681 gcctccgtta aagccaatgg cacaagccag acggtgaaag acaatacgta attacgatgc
    13741 cgggcgtggg ttgttggcgt ccggctttat tttctctcat cacagagtta ctatccctca
    13801 agaaattatt aaccatgaaa tgagttttct cctcataact gataaggaaa attatgctca
    13861 aaggattgcg tacattattt tcacgcaggg ttttgtttaa cgtcgaaatg aataattctg
    13921 ttgactgtga attacttctc atccagcaac aactccaggc attgatagcc gaaccaggca
    13981 attatttagc acatgattgt gatctcaacg atactctggt tctttgccag cttacccatc
    14041 atcacaatgg atatatacga gaaaaggccg tgcagtatct ggggcacaaa gaggatgttg
    14101 cggctataga agaattatta cttgcagcta atgactgggt aaaagaggtt cgccttgctg
    14161 caaccatagc attacgtcat ttaatgaagg ataagaatgc agcggcattt gccgttaatc
    14221 tacccatgat acgtaacctt ctccaatgcc agcgttacga tcaccgtgca tttgtagacg
    14281 agatacttag atatttattg acagaaaata accgcccaca attaacgtat tggcttactt
    14341 gccctgataa aaagctctcc cgcgcggtat tacaaacgct gattgaatac ggttacttta
    14401 atgatgaatc ttcactgctt tcaatactcg ataaacccga tgagggtttg cgaatgctgg
    14461 cgaccaccca ctggctgcgt caacaattac cattaagcga agcggtatta actcgtctgt
    14521 taaaagaccg ctggccgcgt attcgacaag caacgttatt tagcctcact gaccgagcaa
    14581 tagaaatgcc tccggcgttg cacagcacat tactgctgga taacaatatg ttgatccgcc
    14641 tacgcgctaa aaatatgctt cacgaagtca tggatgttcc gcaattctgg cgtcatgttg
    14701 tcacttctgc ggaatatact ccctcacagc gacgggccgc tttatacggg ctggattcca
    14761 ttcatgatcc caatatttta aaactggcag aatggggatt atcacagaat gtattcccac
    14821 tgcgtttagc cgctatgcac attcttgcaa aagctaatcc tcgttgtggc gtcaaagaga
    14881 ctattctgac cacgctggct aacccggacg ccgccgggtt gcgatttatg gttaacatct
    14941 gtgtatggtg tcgcgttccg ctcacgttcg aggaaatacg tcagttgcag gaaaacgcgc
    15001 cgtcagttaa acatgcttgc gcatattgtc ggctgtatca taacttgaac aaatgggacg
    15061 gtttaattct tcttcttcaa tcgcaacaca agctaactga agaatttgca ggtaaacagc
    15121 tcgcgatatg gcaacgcaat ttcaatcttt cgggcattca accgaatgct cttcagcgtc
    15181 agcaacttca ggcgcttttt acccgcaatc cggaacttca caacagatta tggggttaca
    15241 ttccttttaa ataacttcga attatagact tacgatgaca aatgtacctg ctgacgagcg
    15301 gaaccaaaac gaaacaatac gtgaagcttt acgcttgcta cgagaaaaac ttgccgaggg
    15361 actacgcagt ggcgaagccc aaccgtggga aaaggatctg tttttacata aagtgaaggc
    15421 cggtaacaaa taaatccccc agtcttttcc ctcacatttg cactggagcc aaccccgccg
    15481 attgcgctac aatgcccgcc cttaaagtgg gggcactccc ctaaccgctt catcaggtga
    15541 agcggatctg acctgtcatc agaacgagag aattatgttt aaaccggaac tcctttcccc
    15601 ggcgggaacg ctgaaaaata tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc
    15661 gggccagccg cgttactccc tgcgtgtgcg caacaacgaa ttcaaccacg aaaatcttca
    15721 gctcggcatc aatgaagccc acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc
    15781 accgcacaac gccaagctga aaacctttat ccgtgacctg aaaccggtgg tggaaatggg
    15841 gccggatgcg ctgattatgt ccgatccagg gctgattatg ctggtgcgtg agtacttccc
    15901 tgaaatgccg atccacctct cggtgcaggc taacgccgta aactgggcga cggtgaaatt
    15961 ctggcagcaa atgggcctga cccgcgtgat cctctctcgc gaactgtcgc tggaagagat
    16021 tgaagagatc cgcaatcagg tgccggatat ggagatcgag atcttcgttc acggtgcgct
    16081 gtgcatggcc tactccggtc gctgcctgct ctctggctat atcaacaagc gcgatccgaa
    16141 ccagggtacc tgcaccaacg cctgccgctg ggagtacaac gtccaggaag ggaaagaaga
    16201 tgacgttggc aacatcgtac acaagtacga gccgatcccg gtgcaaaatg ttgagccgac
    16261 gctaggtatc ggcgcgccaa ccgacaaagt gtttatgatc gaagaagccc agcgtccggg
    16321 cgagtatatg accgcgtttg aagatgagca cggcacttac atcatgaact cgaaagatct
    16381 gcgcgccatc gcccatgtag aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga
    16441 aggccgtact aaatctttct actattgcgc acgcaccgca caggtttatc gtaaagctat
    16501 cgatgatgcc gctgcgggca aaccgttcga taccagcctg ctggaaactc tggaaggtct
    16561 ggcgcatcgt ggctataccg aaggtttcct gcgtcgtcat actcacgacg attatcagaa
    16621 ctacgaatac ggttattcag tttctgaccg ccagcagttt gttggtgagt ttaccggtga
    16681 gcgcaagggg gacctcgcag cggtagcggt gaaaaataaa ttctccgttg gcgacagcct
    16741 tgagctgatg acgccgcaag gcaacattaa ctttaccctt gagcacatgg aaaacgccaa
    16801 aggtgtagcg atgccggtcg caccaggcga tggttatact gtgtggctcc cggtcccgca
    16861 ggatcttgaa ctaaattacg cgctgctgat gcgtaatttc tccggggaaa ccacgcgtaa
    16921 cccacacggt aagtgattaa tttcgattat ttttcccgga tggaaaattc ttagaaaccg
    16981 atcacataca gctgcattta ttaaggttat catccgtttc gctgaaaaac ataacccata
    17041 aaatgctagc tgtaccagga accacctcct tagcctgtgt aatctccctt acacgggctt
    17101 attttttatg cataagccct atccctggtc accgtcttcc attgaccaca tcgatagaat
    17161 ctcccttcat agcacgatgc ctttcacgta acggcatcgt gctcgcacag gttccggcta
    17221 agcacaacca gaacgcgcat gtttgatgct taccaaaaaa tattctcact ctccacattt
    17281 gaatgtcaga cgagcgacac catgtaatcc tacaccttct gtcttcagct caactatttg
    17341 catttttttg ccctgagtaa cacagaaatg agttgcatca ttttttacta tattttctgc
    17401 accagatatt ctactcctgg ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag
    17461 tttacgctga atattacttt tatatgcaag accaaattta ccgatacttg tctcatcatt
    17521 atgcacagca caaccagaca taataaaaat actaattaat gatatagcag ctatcttttt
    17581 catctcacct ttccccatta aataccaacg acactctcta gtgtttaaat ataataatgg
    17641 ctgatcatta tatttgaata ggattataat aaatgttctg tacaacattt cctacataag
    17701 taggacttac agacatgaag gccctttcca ggtaactcca tggggcttta atatatttgg
    17761 aatagtatat ttaagatgcc actgtttggt tgaatattag gcatatgctc ttttttgaaa
    17821 tttatcggtg gctaccgtta atattcgctg ttcccattgc aagctcctgg tggtaaccac
    17881 tgaatcctcc atacttgaac tgacttttta tcctccgact ttcatcctgt tctgactcca
    17941 ccttttcttt tctgctctac actatctaca gaccaatcat aaaggcacat acgatcatgg
    18001 cagaatttcc cgccagctta ctgattctta atggcaaaag tactgacaat ctacccttgc
    18061 gcgaagcaat tatgctgttg cgtgaggaag gaatgacgat ccatgtgcgg gtcacctggg
    18121 agaaaggtga tgccgcacga tttgtagagg aggcccggaa gttgggcgtc gcaacggtaa
    18181 ttgccggtgg tggcgatggc accattaatg aagtttctac ggcgttgatt cagtgtgagg
    18241 gggatggcat acccgcgctg ggaattttgc cattaggaac cgccaatgat tttgccacca
    18301 gtgtagggat tcctgaggca ctggataagg cgctgaaact ggcaattgcc ggtaacgcca
    18361 ttgcgataga tatggcgcag gtcaacaaac aaacctgttt tattaatatg gcgacaggcg
    18421 gatttgggac gcgtattacc acagaaacgc cggaaaaatt aaaagccgcg ctgggtggcg
    18481 tctcttacat cattcatggc ttaatgcgca tggatactct gcaaccggac cgttgtgaaa
    18541 tccgcggtga aaactttcac tggcaaggtg acgccctggt attggtattg gtattggtaa
    18601 cgggcgtcag gccggtggcg gtcagcaatt gtgtccgaac gcgttaatta acgatggctt
    18661 gctgcaactg cgcattttta ccggcgatga aattcttccg gctctcgtat caaccttaaa
    18721 atctgacgaa gataacccga gtattatcga aggcgcttcg tcgtggtttg atatacaagc
    18781 cccacacgaa atcactttta atcttgatgg cgaaccgttg agcggacaaa actttcatat
    18841 tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtccat tattgcgtta
    18901 atcaaactat tctctcttga accagggaga atccgaaatg ccggatgcgg tgtaaatacc
    18961 ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc ttctgcaatg
    19021 ccagtttatc cacctctgat acagcatcat cggtaataat aatgtcaatt ttttcgattg
    19081 gcagtacctg attaaaaccg cgacggttaa atttcgatga atcgagaacg gcaatcactt
    19141 tattagcggc tgtgaccatc gcaccgctaa cggaataacc ctcattaaag gtcgtaatac
    19201 cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta atatcctgca
    19261 gggaacgttc agcaatagat ccatgcatag atcgcgtttt atggcggacc gtgccaccgc
    19321 agacgaccag agtaatgtct ttattttcgg aaagggcaaa cgctgccggg agactgttgg
    19381 taatcaccgt gatattttta gcggtcatta atccttcggc gataagcatt gtggtacttc
    19441 cgctgtcgag aataacggtc atcccttcat ggatcatttt gactgccgcc tgcgctattc
    19501 tgttttttgg cgcgctggcg agctgaaatc gctctttaaa cccgacttcc tgggtctcgg
    19561 tttcactatt accggacatt attttcgccg caccgccatg aaagcgcgta accacgcctt
    19621 tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca aatactcccg
    19681 ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc ttattccttc
    19741 gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta aacgaagggg
    19801 gaccattggt gaatgatctg ttttgcacaa taccgggcca gcatcacgct ggcccgcggt
    19861 ttcatggaat gagcaacact ttgcccggca tggcattacg agcgatgtca cgcaccgcct
    19921 gggtgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt aacttacgtt
    19981 ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg gaatagttca
    20041 tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt gccgatgtta
    20101 aatgcagatc ctgatgcaac gtgcccacca gcgccagttg ggcatgagga ccggcaatct
    20161 ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc gaggataagc tgattaaaac
    20221 gcagttcacg taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt
    20281 gcatcgaacc gaaagatttt gccagtgcca gtttttctga gctaatgtcg atagccgtca
    20341 cactctttgc tcccagcgcg acagcgcact gaatagccag caggccaatg gttccggcac
    20401 caataataat aacgttttta ttctcacaac cttgcgctaa atgaaaagca tgcagaccaa
    20461 cggtaatcgg ctcaataaaa gccccatcct caataggcat atccgtgggt agagcaaaga
    20521 cattttttcg cttaacgaca atatattcag caaatccacc atcacgccgc gagccaataa
    20581 aatcatattt tgcgcactgg gaataaaacc ctttcagaca ctctggacaa gtaaaacagg
    20641 gtaataacgg cacacaggca accgcatcgc cagggtgtaa atcatcaaca ccggatccca
    20701 ccgcatcaat atagccgcta aattcatggc ctaacgttat tggataataa tgtgcaccat
    20761 ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt tttacccgca
    20821 cctcatcctg atgtttaatt tcaggaatga cgctttctgc aacgcgcacg ataccatcag
    20881 tatcattcac cactgatttc ataaaaactc ctgattgtta agggggataa actccccctc
    20941 agttaaaaat tattctgcga gaacggcttt ctcttgttta ataaaaccac gcgctctacg
    21001 ccaggtaatg aaaataccgg tcagataaat tgcgccgata ataatgaaac cgggaatatt
    21061 ttgcggggag aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga
    21121 agccaccata cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc
    21181 aatagtttgc gtcgcgatcc acagagtgat gctcatgatg atgacacccg agattaaggt
    21241 gcggaacaga tttccacgat gcacggcgac tgccatcgcc acaaagaagc cgatggtggc
    21301 aagatcgcca aacggcagta cctgattacc cggaacacaa acagcaatta aaatggtgag
    21361 cgggataaaa atcaggctta ccgataccac cgccgtatgc cccagcagta atgcgggatc
    21421 caggccaatc aggaactcct gaccgccgaa cttcgcctgt aaacgactac gggcctgctt
    21481 agcgatgggc gttaaaccat ccatgatggg tttaatcacc cgtggcatta gcagcatcac
    21541 tgccgccgtt tttaccgcca gctgcaatac acctttgaca tcgtaacccg cgaggatgcc
    21601 gataatcagc cccatcacaa aacccacggt gacaggttcg ccaaaagggc caaatttgcg
    21661 ctgaatatca tcggcgctaa atttaattcg gttaacgcct gggattttct cgatgatagc
    21721 atcgaccagc accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatacc
    21781 ttccagctca aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa
    21841 cgccgcgtga attaccacac ccgccatccc tatcatccat gaaccggttg ccagatgcag
    21901 caacgcgccg gtgaaagtca tatgccagat attccagata tcaacattta ccacccgcgt
    21961 catacgggtc agtaacatcg ccacgttaac cagaatagca atcggaatcg ccaccagcgc
    22021 aatttgcgac gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag
    22081 attcaggtcg aaattttccg ccatcgcttt cgccgccgga ccaatggaat ccagcattaa
    22141 gccaatcaca aggccaatgc caacaaagcc aatccctata tgaagacccg ctttaaagca
    22201 atcgcctgcc ttcatgccta atattttaga aaaaataatg atgacaatcg gcagcatcac
    22261 cgtagggccg aggtcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa
    22321 gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaacaa aaggcatgcc
    22381 gtgaactaac ggaatatcgc caaaactacg atccaccctg gcagtagtgc atatcaaatg
    22441 cacgccatcc atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat
    22501 attatgattc tgacacaatt ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc
    22561 gcctccgcaa gcgacaataa tcttgcgttt cataaatagt cctttttatt gttacagagg
    22621 gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact gggtttcagg
    22681 aagagtgatt agtgtctcga cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa
    22741 aagtttcaat tgctgctgcg gattttccac aatcaacgca ataaccaacg atacctccac
    22801 gtcgttatca tcatccgctt gctgaaaatg aactttattt gttggcctta acagata
//
LOCUS       DADLTD010000047        22777 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLTD010000047 DADLTD010000000
VERSION     DADLTD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.047"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..454)
                     /locus_tag="JN701_001456"
     CDS             complement(<1..454)
                     /locus_tag="JN701_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7363953.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLET"
     gene            complement(484..1200)
                     /locus_tag="JN701_001457"
                     /pseudo
     CDS             complement(484..1200)
                     /locus_tag="JN701_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1496..2272
                     /gene="exuR"
                     /locus_tag="JN701_001458"
     CDS             1496..2272
                     /gene="exuR"
                     /locus_tag="JN701_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY7363954.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2617..3279
                     /locus_tag="JN701_001459"
     CDS             2617..3279
                     /locus_tag="JN701_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7363955.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3283..3666
                     /gene="mzrA"
                     /locus_tag="JN701_001460"
     CDS             3283..3666
                     /gene="mzrA"
                     /locus_tag="JN701_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY7363956.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3813..4181
                     /locus_tag="JN701_001461"
     CDS             3813..4181
                     /locus_tag="JN701_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAY7363957.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4219..4524
                     /locus_tag="JN701_001462"
     CDS             4219..4524
                     /locus_tag="JN701_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7363958.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSDEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4527..4931
                     /locus_tag="JN701_001463"
     CDS             4527..4931
                     /locus_tag="JN701_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363959.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4921..5220
                     /locus_tag="JN701_001464"
     CDS             4921..5220
                     /locus_tag="JN701_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363960.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5316..5798
                     /locus_tag="JN701_001465"
     CDS             5316..5798
                     /locus_tag="JN701_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7363961.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSKFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            5868..6771
                     /locus_tag="JN701_001466"
                     /pseudo
     CDS             5868..6771
                     /locus_tag="JN701_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7064..7429
                     /locus_tag="JN701_001467"
     CDS             7064..7429
                     /locus_tag="JN701_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7363962.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7671..8027
                     /locus_tag="JN701_001468"
     CDS             7671..8027
                     /locus_tag="JN701_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7363963.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8078..8974)
                     /locus_tag="JN701_001469"
     CDS             complement(8078..8974)
                     /locus_tag="JN701_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7363964.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            9079..9780
                     /locus_tag="JN701_001470"
     CDS             9079..9780
                     /locus_tag="JN701_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7363965.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9803..9967
                     /locus_tag="JN701_001471"
     CDS             9803..9967
                     /locus_tag="JN701_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7363966.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10101..11411)
                     /locus_tag="JN701_001472"
     CDS             complement(10101..11411)
                     /locus_tag="JN701_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY7363967.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKFQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11439..12771)
                     /locus_tag="JN701_001473"
                     /pseudo
     CDS             complement(11439..12771)
                     /locus_tag="JN701_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            complement(13045..14409)
                     /gene="tdcG"
                     /locus_tag="JN701_001474"
     CDS             complement(13045..14409)
                     /gene="tdcG"
                     /locus_tag="JN701_001474"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7363968.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(14481..14870)
                     /locus_tag="JN701_001475"
     CDS             complement(14481..14870)
                     /locus_tag="JN701_001475"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY7363969.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14884..17178)
                     /gene="tdcE"
                     /locus_tag="JN701_001476"
     CDS             complement(14884..17178)
                     /gene="tdcE"
                     /locus_tag="JN701_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY7363970.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(17212..18420)
                     /gene="tdcD"
                     /locus_tag="JN701_001477"
     CDS             complement(17212..18420)
                     /gene="tdcD"
                     /locus_tag="JN701_001477"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY7363971.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(18446..19777)
                     /gene="tdcC"
                     /locus_tag="JN701_001478"
     CDS             complement(18446..19777)
                     /gene="tdcC"
                     /locus_tag="JN701_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY7363972.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19799..20788)
                     /gene="tdcB"
                     /locus_tag="JN701_001479"
     CDS             complement(19799..20788)
                     /gene="tdcB"
                     /locus_tag="JN701_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY7363973.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20887..21825)
                     /gene="tdcA"
                     /locus_tag="JN701_001480"
     CDS             complement(20887..21825)
                     /gene="tdcA"
                     /locus_tag="JN701_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY7363974.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            22014..22358
                     /gene="tdcR"
                     /locus_tag="JN701_001481"
     CDS             22014..22358
                     /gene="tdcR"
                     /locus_tag="JN701_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY7363975.1"
                     /translation="MTRITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5974 a         5761 c         5308 g         5734 t
ORIGIN      
        1 ctgtctccag aacacctttg gtggtttgag caaacatcag gtttttagcc agagaaccgt
       61 tagttaacgc atcggaagca aagattttgg ttgcggtaaa agtggttgcc gcatcagctt
      121 taatttcttc accgccccag gttactgtca gcggaacaat cttgctagca tccaggcttt
      181 gaaccagttg cgccggggaa actaactgca tttttacatc tttggtaacg tcgttagacc
      241 agattttggt catcaactgg taagattgca gaccctgccc cggcagatat tgcatttcta
      301 ctgctttcgg caacgcggtg ttatcggtct gtgtcatatc cagagcggca tcaacgttgg
      361 ccgttacagt aatatccttt tgtactgcca atgcggagcc tgcaacagaa aacatcgctg
      421 cgaccagaag agattttgca aatacctttt tcattactat tccttcgttt attacaatga
      481 taattaatgg accgccagtt caaactcgcc attatctttg gtgatccagt tattatattt
      541 gacaacgatt ttgtcgtagc cagatatgct ttcaagattt ctttcctgac cagggaagat
      601 gtttttattt acggtctgta tcttacaatt ttctttgcta ttatttttac aataagcgat
      661 cgttaaaatc tttaagcgtt gattccctgc attaactaat ttttgggcag catttacttc
      721 taacttagca ataggttgct gaggtggaac actaacaaca atcccccaaa ttaagctaac
      781 agttaactgc atagagaaat cttttttatc tgtggcaacg tcatcaatac tgggaaccgc
      841 ttcaaattta acgcgttaat ttttttcttt ttgctctggt tccattgcaa caaagcgcac
      901 agttttgcta ctaccggctg gtaaagcgaa tttaggtggc ataacaacca gatgatttgc
      961 atcaccggat ttaatctcaa cttcattttc ctgaggcgtc gatggattat cgatacggaa
     1021 tactgtcgcc ttgatgtact gaacttcatt acttttagat attacgcgaa cgttgccttc
     1081 atcctgacta ttaatggaaa cagccatagg ataaacagtc atatttgact gtgccaaaaa
     1141 aggagcgaac acgaataaaa gcggcgcaag ccgtgataag agattaattt tcgccaccat
     1201 ataaatttga tcttacaata ttgtttccaa aaagttggtg tattagagat gtagatttat
     1261 tgtaagtcaa tataaaaaag catttttagt taatattaaa tttaaaggct gggaattaac
     1321 ttgctgaaat ttagtagctt tcacaatgac gatacttatt ttttaggaat ttataaatat
     1381 tcacaatatt aattcaatac attaattcaa tcaccaaatt tatatatgac caatggcata
     1441 cccatgaata aagtggtatg ataacttcgt ccgcattcca ctccgggaga cgcatatgga
     1501 aatcactgaa ccacgccgtt tgtatcaaca acttgccgct gacctgaaag agcgcattga
     1561 acagggcgtc tatctggtgg gtgataaact gcctgccgaa cgctttattg ccgatgaaaa
     1621 gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg ctggaagttg aaggctatgt
     1681 ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac cagccgcgcc atcagcaggc
     1741 ggctgacaat aatatggaat tcgccaatta cggtccgttt gagttgcttc aggctcgcca
     1801 gctcatcgaa agtaatattg ccgagttcgc ggcaactcag gtaacgaaac aagacatcat
     1861 gaaactgatg gccattcagg aacaggcgcg cggcgaacaa tgcttccgtg attccgagtg
     1921 ggatttacag ttccacattc aggtagccct ggcgacgcag aactccgccc tggcggccat
     1981 cgttgaaaaa atgtggaccc agcgtagcca taacccgtac tggaaaaaac tacacgaaca
     2041 cattgattcc cgtaccgttg ataactggtg tgatgaccac gatcaaattc tcaaggcgtt
     2101 aattcgcaaa gatccccatg ctgctaagct ggcaatgtgg cagcatctgg aaaacaccaa
     2161 aatcatgtta tttaacgaaa ccagcgacga tttcgagttc aatgccgacc gctatctatt
     2221 cgccgaaaac ccggtagtgc atctcgatac ggccaccagt ggcagtaaat gaaactcctg
     2281 tccgacaggc gctgcgacgc gcctgttcgc ttcgtcggta agcgaaaagt ataaagtgtc
     2341 agcctgtgta aatcctctcg ccaccctccc ctgcattcag caaaatcaga ctccacggac
     2401 atgtaatttt gataacgaac aacgttgacc tttgttacaa ttagattcaa tttgaattta
     2461 tgtttttgaa tgctttctta tctcacgatt taacagggaa tagttcaggc tgtgttgatg
     2521 tatcaaaccc gcagaacata ccaaaacagc aataacattg cggtagtgca tcttttaaaa
     2581 ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg aacttttgac ccaattgctg
     2641 caagccctgt gggcgcagga ttttgaaacc ctggccaatc catcgatgat tggcatgttg
     2701 tattttgtct tgtttgtaat tttgttcctt gaaaacggct tgcttccagc ggccttttta
     2761 ccgggcgaca gtttactggt attggtcggc gtgttgattg cgaaaggcgc gatgggttac
     2821 ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg gctgctgggt cagctatatt
     2881 caggggcgat ggctgggcaa tacccgcacc gtacaaaact ggctatctca tttacccgcg
     2941 cattatcatc aacgcgcaca ccatcttttt cataaacacg gtttatcggc gctgttaatt
     3001 ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga ttgccgggtt atcagggctg
     3061 aataacgcgc gctttcagtt tttcaactgg atgagcggtc tgctgtgggt attgatcctg
     3121 acaactctgg gttacatgct cggcaaaacg ccggtatttt taaagtacga ggaccagctg
     3181 atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt ttggcctggc aggttctctg
     3241 gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat acctcgcatg
     3301 tcgcttcgcc agctagcctg gtccggcact gttttacttc tggtcggcac gctgttgctg
     3361 gcctggtccg cggttcgcca gcaagagtct acgctggcga ttcgtgccgt tcatcaaggc
     3421 acaacgatgc cagacggttt ttcgatctgg catcaccttg acgctcatgg cattcctttc
     3481 aaaagtatca cccccaaaaa cgacactctg ttaattacat ttgactccag cgaccagagc
     3541 gccgccgcaa aagcggtcct cgacagaaca ttgccccatg gctacatcat tgcgcagcag
     3601 gacaataaca gtcaggctat gcagtggctg acccggttac gggataactc tcatcgcttc
     3661 ggataacttc ctgttatccg aaacatatca ctcactttgg tgatttcacc gtaactgtct
     3721 atgattaatg aggcggtggt taacactacc gcttcgttgt ctggacaatc gttcctttgt
     3781 aacaggtccg aatcacaatg gaaggttcaa gaatgaaata ccgcatcgct ttagctgttt
     3841 ctctctttgc tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa aaggagcaaa
     3901 atatccttaa ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt attgacggtc
     3961 tgaataaagc cctgagtgaa gtccgggcca actgttcaga tagccagctg cgtgccgatc
     4021 atcacaagaa aatcgcaaag cagaaagatg aggtggcgga acgccagcaa gatttagccg
     4081 aggcgaagca aaaaggcgat gccgataaga ttgccaaacg cgaacggaaa ctggcagaag
     4141 cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta actcacaata gtcactactt
     4201 actcacctgg agaaaactat gtcgaaagaa cacactacgg aacatctgcg tgctgagttg
     4261 aaatcccttt ccgatacgct ggaagaggtg cttagctcat ctgacgagaa gtcgaaagaa
     4321 gagttgagta agattcgtag caaagcggag caggcactga aacagagccg ttatcgcctg
     4381 ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg cggcggcgcg tgctgatgag
     4441 tatgtgcgtg aaaatccgtg gaccggcgtg ggaattggcg ctgcaatcgg tgtagtgctc
     4501 ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca tcacgcgcaa gggcccggta
     4561 aaagcgttct gggcatcggg cagcgaattg tttccatcat ggttgaaatg gtggaaacac
     4621 gtctgcggct ggcggtggtg gagctggaag aggaaaaagc taatctcttt caacttttac
     4681 tgatgctggg cctgacgatg cttttcgctg catttggtct tatgagcctg atggtgctaa
     4741 ttatttgggc ggttgatccg caatatcgcc tgaatgcgat gattgccacc accgtggtgt
     4801 tgctgctact ggcactgatt ggcggtatct ggacgttacg taaatcgcgt aaatccacct
     4861 tgctgcgcca tacacgccat gagctggcaa acgatcggca actgctcgag gaggagtccc
     4921 gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac tgcttagcca gatccagcaa
     4981 caacggctgg atctttccgc cagtcgtcgt gaatggctgg aggcaacagg cgcttacgat
     5041 cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc tggttggcag tagcgtgatg
     5101 gcgatctgga cgattcgcca tcctaatatg ctggtccgct gggccagacg cggttttggc
     5161 gtatggagcg cctggcgtct ggttaaaacg accctcaagc agcaacagct tcgcggttaa
     5221 cttccctctg gccggagcca ttccggccct attcctcaaa ttttttgaag atttttgaca
     5281 gttttccttg ctaacaatca tcattcacca cgtttatgat tctctccatc gacagcaacg
     5341 acgctaatac cgcgcccttg cacaaaaaaa caatcagcag cctgagtggc gcagtggaga
     5401 gtatgatgaa aaaattagaa gatgttggtg tactggtagc gcgcatttta atgccgattc
     5461 tgtttattac cgcaggctgg ggaaaaatca ccgcttactc tggtacccaa caatatatgg
     5521 aagcaatggg tgtcccgggt tttatgctgc cgctggtgat tctgctcgag ttcggtggtg
     5581 gtctggcaat cctgttcggt ttcctgactc gcaccacagc cctgtttact gcgggcttta
     5641 cgctgctgac ggcattttta tttcacagca agtttgctga aggcgtcaac tcgctgatgt
     5701 tcatgaaaaa cctgacaatt tctggcggat tcctgctgct ggcaattacc ggtccgggcg
     5761 cgtatagcat cgaccgcctg ctgaataaaa agtggtaagc acgcgctata cttaacgata
     5821 aaaaacgagg aggaagctcc tcgtttttgc tattggagga gagaaaaatg gggcaactga
     5881 ttgacggcgt ctggcatgac acctggcccc actggcacag gcggttttat cgcagagaaa
     5941 gatcgttatc atctctatgt ttcactcgcc tgcccgtggg cgcaccgcac gctgatcatg
     6001 cgcaaactca aaggactgga accgtttatt tccgtttccg tagtgaaccc gctgatgctg
     6061 gaaaacggct ggacctttga tgacagtttt ccgggagcaa ccggagatac gctctatcaa
     6121 catgaatttc tgtatcagct ttatctccac gccgatccac actacagcgg acgagttact
     6181 gttcccgtgc tgtgggacaa aaagaaccac accattgtca gcaacgaatc agcagaaatc
     6241 atccgcatgt tcaataccgc gtttgatgcg ctgggcgcaa aagcgggtga ttactaccca
     6301 ccagccctgc aaacgaaaat tgacgaactt aacggctgga tttatgacac cgttaacaac
     6361 ggcgtgtata aagctggttt tgccaccagc cagcaagcct acgacgaggc ggtggcgaaa
     6421 gtgtttgaat cgctggcgcg actggagcag attttaggcc agcatcgtta cctgaccggc
     6481 aaccagctaa ccgaagccga tattcgcctg tggactacgc tggtgcgttt tgatccagtg
     6541 tatgtgaccc acttcaagtg tgataagcac cgcatcagcg attacctgaa tctgtatggc
     6601 ttcctgcgcg atatctacca gatgccgggg atcgccgaaa ctgtcaattt cgatcacatc
     6661 cgtaatcatt acttccgcag ccataagacc atcaacccta cggggattat ttcaattggt
     6721 ccgtggcagg atctcgatga accgcatgga cgagatgttc gcttcggtta aaaaaaggca
     6781 ccttacgagg tgcctctgat aattagctaa gtgcatgttg ttcatgtcgg atgcggcgtg
     6841 aacgccttat caaacctaca aaacgtttat attcaatatg ttaaaatgac cgcgtaggcc
     6901 agataagcgt agcgcatctg gcgattttgc gcttgtcatc agtcttagcg caccttcacg
     6961 gcactttatt aattccttga acgaatattt actgccattt cattcacgtc tattcttaat
     7021 ttgctgcttt aaaaacaagt gattgagcaa aattgaggca aaaatggact ggtatctgaa
     7081 agtactaaaa aattatgtcg gtttccgggg tcgtgcgcgg cgcaaagagt actggatgtt
     7141 tattctggtc aacatcatct ttacgttcgt gctggggctg ctggataaaa tgttaggctg
     7201 gcaacgcgcc ggtggcgaag gcatcctgac gacaatctac ggtattctgg tgtttttacc
     7261 gtggtgggcg gttcagttcc gccgcctgca cgacaccgac cgctcggcgt ggtgggcact
     7321 gttgttctta atcccgttta tcggctggct tattatcatc gtctttaact gccaggcggg
     7381 tacgccaggc gaaaaccgct ttggaccaga tccgaaactg gaaccttaaa acaaatctgc
     7441 tcgccggatg tctgactcat ccggctttta tcatcatgtt gatgaatgaa taatttacta
     7501 atgtttattt aaaatatttc aatgtcgtta tataaattac ctataaaaaa caaccatgta
     7561 tttatcttaa atatcaaaac ctttataaca tccgaaaaag aaagatacga atagcattta
     7621 cacaataccc ggaatagact ttcgtcactt ttaattaaag ggatgttttt atgcagtggt
     7681 atttagccgt attaaaaaat tatgttggtt tctctggtcg cgcacgtcgt aaagagtact
     7741 ggatgtttac tctgattaac gccatcgtcg gcgctattat caatgtcatt caattgattt
     7801 taggtttaga gtttccattc ctttctctca tttacctggc agcaacaatc attccggtca
     7861 ttgctctgtg tgttcgtcgt ctgcacgata ctgaccgttc aggtgtgtgg gcgcttctct
     7921 atctggtacc gatcattggc tggttagttc tgtttgtatt tgcctgttta gaaggcaatt
     7981 ctggcagcaa tcgttacgga aacgatccta agtttggttc aaattaatct tagaattggg
     8041 gcgatatttc gccccttttt attaacaata ataaatatta ttttccgcta aaaagtttgg
     8101 gaatttcccg cagacaccag gatttcgctt cccccatact gtcacgacgc caggccataa
     8161 taatatcgat ctcgctggtc gattccgggc tgacgacacg caaccgccct tccgcaatat
     8221 ctttttcgac catcggatac ggcatcgtcg ccacgccaag ccccgccagt aatgcctgac
     8281 gtttatcttc aatcgtgctc accgttaagc gcggctgttt gtccagcagc tgtacggtca
     8341 acaccgggcg ctcacgagcg gtatccgcta ccgcaattcc acgatatttc acgcgcgtca
     8401 cttcagataa cggttccggc tcctgatgaa tcgggtgatc aggcgcggca acgtagacgt
     8461 tcattaaggt atagagcttg cgcgagttga tctccgacga ggaacgaaaa tgcatatccg
     8521 gcgcgataac gatatccgcc cgcccctgct ccagtcgttc ccacgccccc gccagcactt
     8581 cggtgatgat tgccagttgg gtattggctt ttgccgccag tttgtcgatt aacgggaaaa
     8641 aggcaggtgt cggtaccagc gcctcggtca caatggtgag atgcgtttcc caaccgcgcg
     8701 cgagggcttc cgcatcggta gtcagtttat ctgctgcttc cagcaaaacg cgccctcgct
     8761 ccagcaacat cctcccgaca ttggtgaatt tggtgcgatg gcccgagcgg tcaaacagca
     8821 ccacatccag ctcttcttcc agtttttgca tggtgtagct aagtgcggaa ggcacgcgtc
     8881 ccagctcatc cgccgccgcc gcaaaactgc cccggcgatc gatcgcatcc ataacccgta
     8941 gtgcttccag cgttaatgcc ctttctttgg ccatttcgtt ctcattcagg aaatttgaac
     9001 ataccgggca gaatatctgg ctaacaatgt agcgtccagc cccttaccat aaaaggaagt
     9061 aaagagaggt caagaattat gattactacc cgaactgcca ggcagtgtgg acaagcagac
     9121 tacggatggt tgcaggcccg gtatactttt tcctttggac actacttcga cccgaaattg
     9181 ttaggctatg cctccctgcg tgtgcttaac caggaagtgc tggccccagg tgccgccttt
     9241 cagccgcgaa cctatcccaa agtcgatatt ttaaatgtga ttctggatgg agaagcagag
     9301 tatcgcgaca gcgaaggcaa tcatgttcag gccagcgccg gtgaggcgtt gctgctctct
     9361 acccagccgg gtgtcagcta cagcgaacac aatctcagca aagacaaacc gttaacacga
     9421 atgcagcttt ggctggacgc ctgcccgcag cgagagaatc cgctgattca aaaactggcg
     9481 cttaatatgg gcaagcagca attaatcgcc tcgccagagg gtacgatggg aagcctgcaa
     9541 ttacgccagc aagtgtggct gcaccatatc gtgctcgaca aaggcgaaag tgcgaatttc
     9601 cagttgcatg ggccacgcgc gtatttgcaa tcgattcacg ggaaatttca tgcgcttacg
     9661 catcatgaag agaaagcggc gctgacctgc ggtgatgggg cgtttattcg tgacgaggct
     9721 aacattacgc tggttgccga ttccccactg cgcgctttgc tgatagattt gcctgtctag
     9781 ttgtttttac agggagatga tgatgagtaa aaaatcggcc aaaaagcgcc agccagtgaa
     9841 gcccgtggtg gcgaaagaac ctgctcgcac cgccaaaaat tttggctatg aagagatgtt
     9901 gagcgagctg gaagccatcg tcgcggatgc tgaaacgcgt ttagccgagg atgaagctac
     9961 cgcgtaaaga caggttgccg gatgcggcga aaacgcctta tccggcctac aaaatccaac
    10021 aacattaacg aattaaacaa cccgtaggcc ggataaggtg cgccagcatc gcatccggca
    10081 caatactcaa aacctggcgt ttatctggcc ttgctcgcca taatctcgat aatctgccga
    10141 tccgtttgct gcatcgaatg gcttgctaac gcacacaggt tggcaatcga ctgctcaaca
    10201 tcatgcgcca cgatcccttc attgccggtc acggcggtat catccagcgc cattaacacc
    10261 gctttccacg cagccgaagc actggtcgaa accttcatcg cgcagctgtt cgacgcacca
    10321 tcgcaaatca tgccgctgac atcgccgatc atactgctga tcgccatcga gatagtttca
    10381 taacgcccat ccaccagcca tgccatcccg gcggcggccc ccattgctgc ggtcgttgcg
    10441 gcacaaagtg cagacaaacg cggtaactgg ttatggatgt aaattgcgct caaatgagaa
    10501 agcatcagcg cacgcgccag tcgttcatca tccgctccga agtgttctgc tacaaccacc
    10561 acaggcattg ttgcggtgat cccctggtta cccgagccgg agttactcat agccggaagc
    10621 gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac gaatcacaat ggatgaagag
    10681 agatctttcg ccagcaaacc gcgctcgcac tgtttttcca gcgtcgcgcc aatatgcagc
    10741 ccccacttac cgctcaaacc ttcctgcgat aacgcacaat ttagcttcgc ggaatcgaga
    10801 ataaagcgga tcgccgcaaa cgggacttca ttgacgaact tcaggatctc agccagcgtc
    10861 gttctggaaa gcaccgtcag cggagactct tgctcgccct ctgccacaca cgcctgctgg
    10921 gtaaacacca cactattgtg cgtctcaata tgcacaatgt tggtatgccc gccgacgatg
    10981 gtgacacacg cccacttctc accgttccag actttggcgc gtgagaagag gatttcattg
    11041 caaggttcct ggaacttaac ggagactttc cccgccgcca gcagtgcttt ggcatcggca
    11101 attgcctgcg cagttgcgtc tttcagcact tccagcccgg cgttggcatt tccacctaac
    11161 gcccccagcg ccgccgcaat cggcagcccc accattcccg tgccgggaac ggtgacgccc
    11221 agaccgttct tcatcagatt tggcgaaacc caggcttcta cacgttcaac cggaccttcc
    11281 agttctgccg cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt acatcccagc
    11341 gccggtttta cttcctcctg aacggcgagg atgtaacgct gccataacgg atttaaagtc
    11401 gaatcaaaca tattaaaaac cttaaaattt caggtaaatc aagaaaatgc caggaacgga
    11461 gaaacacaaa gcaacaaacc agtgacgata atcaggtaca gagacatccc tttgtatttg
    11521 tgcaatgccg gtactttgta aaccagccac gccgggatca ggcaccctac catgccgaaa
    11581 atcgggctac agatagaggt gaagctcaac accggtgcgt tcagtacgat ggcgctccag
    11641 gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa gattttcgtt aatcttctcg
    11701 gcaggcatct tgcgacgcag gatgttcatt acgatccctt gcgttgcttc gcgaaagcct
    11761 aaatagacgc caaagaatgc agtcatgaca gcaaagatat tgagaatgac gctgaccact
    11821 ttcacccacg ctgcgccgtc accgctaata aactgtgcgg caatcgccag cgcggaaata
    11881 ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg ccagcgtgaa cgacacggcg
    11941 tagaaaaaga cggtgacaaa taaaatgcca aacgcgatat tcattgcccg caatgcttta
    12001 tgccgcgcca cttcaattga tttttcccgc gagcgataag agatcaccat cgggcttaac
    12061 gtctggataa acagaatcga cgtcagggta aacggcagcg taataatggc gtttttcacc
    12121 agcagcccca gcggcggtag cgaaccgacg ttatacagat gccacattcc caccatcgac
    12181 acgcccagcg cagcgactac cagcagcttg gtcagcacca tgccggtcga aattttgaat
    12241 aacaatttct cgccgcgtga agagatcgcc accagaatgc aaatcagtac cagaccataa
    12301 aagggactgt ctgacagcac cccttccgtc acgccgaagg tatgcagata ggaagcacta
    12361 tcgttggtga tggcggtgga atagacgaac atccaaatca ccagcattac gaaatagagc
    12421 gcacctaaca ggatgcccca gtttttacct aaataaccgc taatgacact cgggtaatct
    12481 ttacattctg tgtgattctg ccagcgtatt aataaacaac cgctgaaaca gatacattgc
    12541 cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac cgacctgcac
    12601 cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca cccagccggt
    12661 gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc cagcacgacc
    12721 cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca taatttctcg
    12781 ctcaatattt tgtagggctt attatttttt tccgagccgc atcaaggcga tatgcggtct
    12841 acgtttttgc aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc agagaaaatc
    12901 ttcacaatca agactttgaa ataacggaga tggataagaa ttttctacta aattaatcgc
    12961 aagagaatat tcattctcta tttgcggcag atcacaaaaa aaggtgcaca tttgtgcacc
    13021 caaggatgaa agctgacagc aatgtcagcc gcagaccact ttaatggcca gtcctccgcg
    13081 tgatgtttcg cggtatttat cgttcatatc tttgccggtt tcatacatcg tctcgatcac
    13141 tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc gccatccgcg cggcgtttac
    13201 tgctttcacg gcattaatgg cattacgttc aatgcacggg atttgtacct gtccggcaac
    13261 cggatcgcag gtcagcccaa ggttatgctc catcgcgatt tccgccgcat tgcatacctg
    13321 cgccggactg ccgcccagta gttcagttaa ccctgccgcc gccattgaac aggccacgcc
    13381 aatctccccc tgacagccga cctccgcgcc agagatggag gcgttcattt tatacagcgc
    13441 gccaatagcc cccgctgcca gaaaatagcg ggcaattgac cgctcgttta ccggacgacg
    13501 gaacttatcg taataagcca gcactgccgg aataatgccg cacgcaccgt tagtcggtgc
    13561 cgttaccacg cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt acatgttgat
    13621 ccagtcgatg acattcatcg gatcgttaga gatgttatcg ctagaaacca gctgacgacg
    13681 cagcgctacg gcacgacgcg gcacattgag cggaccaggc agcacgcctt cagtgttcat
    13741 cccgcgttca ataccgtcat gcatcacttg ccagatacgg gcaaaccctg cgtcaatttc
    13801 cgctttgctg cgcagcgcca gctcgttgtg catcatcagg ccagatatag acaggccgtt
    13861 gtaatcacac attttcagca gttcacctgc tgagtggaaa tcgtaaggta cggacgtttc
    13921 gacatcgtgc gacaggccga agtgttcttc ttcgacaata aacccgccgc cgacagagta
    13981 ataggtttta cttaatagcg cttcctgtcc cttccaggca gtgatccgca ttccgttctc
    14041 atggcgaggc aacatttcgg gatggaagat aatgttcttt gctacaggaa aatcaacaat
    14101 atgcgcacca gatgccactg gcagccgccc gctgcgcgtt actaactcta taaatgcagg
    14161 gatctcatca atgacaacat cctgcggact gtttcctgcc agtcccatga taatggcgac
    14221 atccgtggca tggcctttgc ccgtcagtga caacgcccca tacagatcga ccacaatatg
    14281 gctcgtcgcg gttaataagc cgctactttc cagccgatca ataaaacttt ttccggcatt
    14341 cattggcccc acggtatgcg aactggaggg accaatccca attttgaaaa tatcgaatgc
    14401 actaatcata tccacaccct cggattgccg ttcagtgaag tggagcggaa cgaccttgcg
    14461 accgtcccgc tcacgaggct ttacgcacta cgtactgcga tagcttcaat ttccagcttc
    14521 acatctttcg gcaaacgcgc aacctgcaca cagctccggg tcgggtaggt cgcctgatgc
    14581 tcatcgaaga actgcttata gacttcattg atggtggcaa aatcattcag atcggtgatg
    14641 aacacggtca tcttgatgat atcgcccacg ctcagcccgg cagcaaccac gatcgctttg
    14701 acgttttcga ggcttaaacg cgcctgatct tgcacatcag ccgggatctc accggtctgc
    14761 gggcaaaccg gaatttgccc ggaggtgaag accatgctgc ctaaatcaac gccctgaaca
    14821 taagggccga ttgcgcctgg ggcacgttgc gtttcgataa tctttttcat acatcctccg
    14881 gcgtcagagc gcctgggtaa aagtacgtga aataacatcc tgttgctgtt cacgggtcag
    14941 tgcgttgaag cgcacggcgt agccagagac acggattgtc aggttaggat atttttccgg
    15001 gtgctcgatg gcatccagca gcatttcccg attcattatg ttgacgttga ggtgttgacc
    15061 gccttcgaca tccgcttcgt ggtggaaata cccatccagc aggccgacaa ggttggtttt
    15121 acgtactgga tcttctttgc ccagcgccgc aggaacgatt gagaaggtgt acgagatccc
    15181 atctttggcg taggtgaacg gcagtttcgc caccgacgtc aatgaggcca cggcaccttt
    15241 gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg cgcgacgacc
    15301 gtccggcgta ttaccggttt tctggccgta caccacgtta gaagtgatag tcagaatcga
    15361 ctgggtaggg acggcgttgc gataggttgg cagcgcttta attttcttca taaagcgttc
    15421 aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact gcggatattc
    15481 accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca cggattggtt tcacgcgggc
    15541 atatttgatg gcagacaggg agtccgtcgc caccgacagg cccgcgatgc cgcatgccat
    15601 agtgcgatag acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc tgtacttgtc
    15661 gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc accgccagcc agtccatgaa
    15721 gtgatcgagg ctgtccatca ctttgtcgta atccagcacg tcgtccatca gcggtgctgt
    15781 tttcggcccg acctgaatct tcagcttctc gtccaccccg ccgttaattg cgtagagcag
    15841 cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa tcaccattgg
    15901 gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag tcagtacgca tcagatcgtc
    15961 attttcatac tgcaaggaag aggtgacgat cgacacctgc gcggcatatt ttttgaaggc
    16021 aatcggtaat tcttccgacc aaagaatggt caggttaggt tccggtgccg gccccatagt
    16081 gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac cgtccagccc
    16141 catcccgccg atcacttccg tcgcccagat tgggtcgccg gagaacagcg aatcaaattc
    16201 cggtgtacgc aggaagcgta ccatacggat cttcatgatg aagtgatcga tcagttcctg
    16261 tgcctgctgc tcattgagta cgccagcttc aaagtcgcgc tcaatgtaga tatcgaggaa
    16321 cgatgccgtg cggcccagcg acatcgcgcc gccattttgc gatttcactg ccgccagata
    16381 agcgaagtag agccactgca ccgcttcttg cgcattctgc gccgggcgag agatatcaaa
    16441 gccatatttc gccgccattt cctgaatctg caacagcgca tgacgatgct ctgccagctc
    16501 ctcacgcaga cggatggtgg cttccagatc ctcgcctttt tccagacgag actggagatc
    16561 ggcaaattgc agttcgcgtt cacgtaccag ataactgatg ccatacagcg ctacgcggcg
    16621 atagtcaccg ataatgcgcc cacggccata gccatctggt aaaccggtca gcacgccaga
    16681 tttacggcag cgcagcatat ccggtgagta aacatcaaat acgccctggt tatgggtttt
    16741 acgcagatcg gtaaacagat attcaaattc actgtccatt tcacggccat aggcgtggaa
    16801 tgaactttta atcatattaa tgccaccgaa cgggtgtagc gcacgtttca acggcgcatc
    16861 cgtttgcagg ccaacaattt tttccagcgg ctggttaata tatcccgcat catgagcggt
    16921 aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt tttcgatacg
    16981 gataccttcc attacttttt cccacaattc cgtggtggca ggcgtcgctt cggcgaggaa
    17041 agattcatcg ccttcatacg gtgtatagtt atgttgaata aaatcgcgga cattaatttc
    17101 gtttttccag tccgtacctt taaagccaag ccatgcgtcg gcgtacagct tatcgctggt
    17161 atcaatatct accttcatga aaaataatct ctctacaata cttcaactaa attatgcaaa
    17221 ttctgcgggc gcgttaactt tgcctaaatg aatggcatcc aaagcaatca ttttttcttc
    17281 gttagtcgga ataacagcgc aaatgacacg cgcattttca ctggaaacaa ttcgctcacc
    17341 acaggagtta gagcgattat tcatttctgt atcaatctct acgcctaata cagccaaatg
    17401 ttccatgacc agacgacgaa ttaagcttga attctctcct attccgccgg tgaatataat
    17461 tccatccagg cgacgtaatg aagctgcgtg tccggcaata tgacgggcaa ttcggtgaac
    17521 aaaggtttta attgccagtt gcgcgcgttc gtgaccttca tgccaggctt tttccagaac
    17581 acgtaaatcc gaagaaagac cggaaatacc taataatccc gactctttat tcactacgcg
    17641 ttccaggtca cccaagctct ggttggtttg gctggcgacc caggacatcg caccaaaatc
    17701 gacatcgcca ctgcgggtac ccatcatcaa gccttccagc ggcgtcattc ccattgaggt
    17761 gtcaacactc tgaccgttgc gaactgcgca gattgacgcg ccattgccaa gatgcgccac
    17821 aaccaggccg gagtcatctt ccgctagatt cagcaaagaa tgtgcgcgct gggaaacata
    17881 gcggtgcgac gtgccgtgga aaccataacg gcgtacacct aactcttcat aatatttcca
    17941 cggcaggccg tataaataag cttccggagc catcgtctgg tggaaactgg tatcaaatac
    18001 cgccacctga gttacgcccg gaaataattg ctgcgccgat tcaataccac ttaaattggc
    18061 gtaattatgc aggggtgcca gtggagaaac gcgacggata ttatcaatga cctcatcggt
    18121 aataatggcg gactcggtaa aaatactgcc gccgtgagcg atgcggtggc caattaaggc
    18181 cacactgtca tttaaattcc gtttttccag ttcaaatgca attgccttca atgcaccttc
    18241 gtagctgtgg tgagccagcg gtgctggctc tcccccattt acggataaga atgcattttc
    18301 tgagttaata ccgtcggcaa tacctgacat taatacttca cagtcgctgg catcgagtac
    18361 ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa attcattcat
    18421 ctcttttctc atcctgagtt acggattaaa acagtttgta tacgatgttc aggatggtca
    18481 gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc gccagagacg
    18541 gtgctttacg gatggcatac atcggcaaca ggcacagcag ggatgcgata atcggtgcgc
    18601 ccatggcttc aataaggtcg aggatgttcg ggttggcgta ggcaacaacc caggtggagc
    18661 ccatgatgaa gatcatgctg atagtgttca gtttacccag cgacacttta gttttgtcgc
    18721 ctttataacc gaacttcaga atcagaccat tcaagccttc cagcgtcccc agatagtgac
    18781 cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat tccagtgtaa
    18841 tcgcgaacgt tgttttggta ccggtcatgg acgcaaagtg gttagccaga taagaaagca
    18901 ctggaatatt ctgcgctttg gcttccgcca tgttggccgg agacagcgta aacaggcagc
    18961 taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa atgatttggg
    19021 aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca cgtttagaaa
    19081 ccacgaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg gaaatcccca
    19141 gccagacagt gatcaggata ccgtcatgac cggttaacga cagcgaaccg aggtcaacct
    19201 ggtcgataac tgcagagttc cagtaaggga tcagcgacag agaaatcagc accaggctgg
    19261 cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta ccaaaccaga
    19321 tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc agcggtgcaa
    19381 atccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg ccataaatcc
    19441 acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg ccagttttac
    19501 caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg gttagagcca gaaagacaca
    19561 gacgcgccag cgcccggtgg caataaaatg cgatggggta tgccaatacc aacatcagaa
    19621 gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc ccggcgccga
    19681 ttgccgtacc aaacaagcct aacgtccatg tggtatctga tttacgccag gacgattgtt
    19741 ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa cgaattaatt
    19801 aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag atcgatattg ccgccggaaa
    19861 taatactgac ggtttttctg ttttgaatat attggtctaa tttaccgctt aataatgcag
    19921 cacatgccag agcacctgcg ccttcggtga cgactttatt gcgctgaatt aaggcaatca
    19981 tactgtttct gatttcgtct tcgctgacca gcacgatgtc atcgactaat tcacgaacga
    20041 tttcgtaagt taaattaccc gggcgggaga catcacaacc atccgccagg gtgccggtag
    20101 ttcggtgcgt ggttatttct ccggagtgga aagaagccgc catgccgtga acgttttctg
    20161 actgtacgcc aataacacga atggtcgggt taatagattt aattgccacc gcaataccag
    20221 caattaaacc accaccacca attggcacaa tcacgttatc gacatcatag agatcttcca
    20281 taatttccag accaatcgtt ccctggccag caatcacttt cggatcatcg taaggtggga
    20341 taaaaatacg accttccatt tcgacaattt cgctcacttt agcgatagtg tcgttgaagt
    20401 tatcaccatg cagaacgact tctgcggagt agtcgcacgt tgccgctact ttggattttg
    20461 gcgcaccttt tggcatcacc actttaccgt cgatacccag catcgcgcag gagagcgaaa
    20521 ccccttgcgc atggttgccc gcagaacagg ccaccacgcc tttgcgtttt tccgcatcgg
    20581 tcagtgaact taatttatta aatgcgccac gaattttaaa tgaacccgta cgctgcatat
    20641 tttcaaactt caggaatatt tcacctttgc aacgttcact aaaatagttg gagcgaggca
    20701 tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaataata tcatcaatag
    20761 caaccggcag atcgtatgta atatgcatta taaaacctct tcgcctgaat taaatatgta
    20821 gataaccgta accgacacct gcaaaacaga caggtggatt atttatgttt aaataaaaac
    20881 aaataactaa ccaacttcta ttaatagcct tcgtctacac ccattataag atgaatactc
    20941 tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt tcgaccatac
    21001 cgcggcatat tgtgccacag gtaatgtttc ttcaactgga atagtaataa attgatttga
    21061 accaaaaggt gacgtcatat cacaaggaat tacagttaag aaatcagcat tgagaacaag
    21121 attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga tgccatttct
    21181 ttgtaacgta gtaagaagct cgctgtagta ccccatattg gtttgtggca acacccactg
    21241 ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact tactggctac
    21301 cagcacaaac tcggactcga acagcggctc aacatgtaaa tcctgaagct tcatttctgc
    21361 acttaacgta ccaatcgcaa aatccagtcg accgtcgcgg attgccggta agaacgaaga
    21421 cagttgcgct tcatacatag aaacctgcgc tttcgggaac acctctttga acttgttgat
    21481 catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga cttccaccac
    21541 cgcctcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac gggtaatgga
    21601 ttcggaacgg gagagtaaca attgaccggc aggtgttaat gttacaccgg tatttttccg
    21661 caccactaat tccacaccaa aataatcttc aatatcgtta atgattttac tgacggccgg
    21721 ttgagttaac cctaattctt ttgcagccga gccgatagag ccacttctaa tgacttcctg
    21781 aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga cctacgttaa
    21841 ttacctcatt gacggcatga agtgtatcaa aatgaaatga acaggatatg tgcgaccact
    21901 cacaaattaa ctttcattac tttccagggt atcgttatta caattaaata tctttaaata
    21961 tcaacaagtt aaaacataaa aatcagcata aaccctgatt tttatcaaaa aaaatgacga
    22021 ggataaccat attttatgga gataacatca ttcgttatgt ggttaataca aaaaaggggc
    22081 tgagaccata tttcaagcaa ttaccggata attaccaggc aaaatttgag ttaaatttaa
    22141 tgagcaagtt ctcaaatttt attataaata aaccattttc agcgataaat actgcggcac
    22201 gtcacatttt ttcacgctat ttgttggaga acaaacattt attttatcaa tattttaaaa
    22261 tttcgaatac aggtattgat catctcgaac aattgattaa cgtcaacttt ttctcttctg
    22321 acaggacgtc attttgtgaa tgcaatcgtt ttccataaat tcttctcccc tcataggcga
    22381 cgaatagcat tttgtgttga ggatcacaaa acaaataatt gctggtcgcc gcgataaggt
    22441 cagacgaagt caacaagggg aattttcact gtgctttaca tcagcgtagg acacagaatg
    22501 ttgcttcgtt tactgtaacg ccgggtaaat gagcgttttt tgatagtgcg taagtgtcgt
    22561 gcgaggaaaa taagcatcta ttattgtttg tcatgacgac aaggcaggca tcaatgaaag
    22621 gcttcccaat tgcacatatt tttcaccctt caatcccgcc aatgcacgca gtggttaaca
    22681 atcacaatag aaatattgat tattggacgg taaaaagaaa gtttgcagaa attgtctcca
    22741 ccaatgacgt taaaaaaatt tacagtataa gtaatga
//
LOCUS       DADLTD010000048        22655 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLTD010000048 DADLTD010000000
VERSION     DADLTD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.048"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..146
                     /locus_tag="JN701_001482"
     CDS             <1..146
                     /locus_tag="JN701_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7363976.1"
                     /translation="QHKLLQTARLWLARHNGSFDTVDCRFDVVAFTGNEVEWIKDAFN
                     DHS"
     gene            166..756
                     /gene="diaA"
                     /locus_tag="JN701_001483"
     CDS             166..756
                     /gene="diaA"
                     /locus_tag="JN701_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY7363977.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            766..1341
                     /gene="yraP"
                     /locus_tag="JN701_001484"
     CDS             766..1341
                     /gene="yraP"
                     /locus_tag="JN701_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY7363978.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(1455..2495)
                     /locus_tag="JN701_001485"
     CDS             complement(1455..2495)
                     /locus_tag="JN701_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7363979.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQHYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAICLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEARSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(2568..3203)
                     /locus_tag="JN701_001486"
     CDS             complement(2568..3203)
                     /locus_tag="JN701_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY7363980.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            3331..3849
                     /gene="yhbO"
                     /locus_tag="JN701_001487"
     CDS             3331..3849
                     /gene="yhbO"
                     /locus_tag="JN701_001487"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY7363981.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(3829..4272)
                     /locus_tag="JN701_001488"
     CDS             complement(3829..4272)
                     /locus_tag="JN701_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY7363982.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            4323..4625
                     /gene="yhbQ"
                     /locus_tag="JN701_001489"
     CDS             4323..4625
                     /gene="yhbQ"
                     /locus_tag="JN701_001489"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY7363983.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(4612..5115)
                     /locus_tag="JN701_001490"
     CDS             complement(4612..5115)
                     /locus_tag="JN701_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7363984.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(5109..5633)
                     /locus_tag="JN701_001491"
     CDS             complement(5109..5633)
                     /locus_tag="JN701_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY7363985.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            5842..6822
                     /locus_tag="JN701_001492"
     CDS             5842..6822
                     /locus_tag="JN701_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7363986.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            6831..7709
                     /locus_tag="JN701_001493"
     CDS             6831..7709
                     /locus_tag="JN701_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7363987.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            7915..8922
                     /locus_tag="JN701_001494"
     CDS             7915..8922
                     /locus_tag="JN701_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAY7363988.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(9040..10284)
                     /gene="mtr"
                     /locus_tag="JN701_001495"
     CDS             complement(9040..10284)
                     /gene="mtr"
                     /locus_tag="JN701_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7363989.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(10438..12327)
                     /locus_tag="JN701_001496"
     CDS             complement(10438..12327)
                     /locus_tag="JN701_001496"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY7363990.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(12320..12400)
                     /gene="yrbN"
                     /locus_tag="JN701_001497"
     CDS             complement(12320..12400)
                     /gene="yrbN"
                     /locus_tag="JN701_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY7363991.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(12507..13391)
                     /gene="nlpI"
                     /locus_tag="JN701_001498"
     CDS             complement(12507..13391)
                     /gene="nlpI"
                     /locus_tag="JN701_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY7363992.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(13500..15635)
                     /gene="pnp"
                     /locus_tag="JN701_001499"
     CDS             complement(13500..15635)
                     /gene="pnp"
                     /locus_tag="JN701_001499"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY7363993.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(15883..16152)
                     /gene="rpsO"
                     /locus_tag="JN701_001500"
     CDS             complement(15883..16152)
                     /gene="rpsO"
                     /locus_tag="JN701_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY7363994.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(16301..17245)
                     /gene="truB"
                     /locus_tag="JN701_001501"
     CDS             complement(16301..17245)
                     /gene="truB"
                     /locus_tag="JN701_001501"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY7363995.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(17245..17646)
                     /gene="rbfA"
                     /locus_tag="JN701_001502"
     CDS             complement(17245..17646)
                     /gene="rbfA"
                     /locus_tag="JN701_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY7363996.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(17810..20458)
                     /gene="infB"
                     /locus_tag="JN701_001503"
     CDS             complement(17810..20458)
                     /gene="infB"
                     /locus_tag="JN701_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7363997.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(20483..21970)
                     /gene="nusA"
                     /locus_tag="JN701_001504"
     CDS             complement(20483..21970)
                     /gene="nusA"
                     /locus_tag="JN701_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY7363998.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(21998..22450)
                     /gene="rimP"
                     /locus_tag="JN701_001505"
     CDS             complement(21998..22450)
                     /gene="rimP"
                     /locus_tag="JN701_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY7363999.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         5336 a         6325 c         5649 g         5345 t
ORIGIN      
        1 aacaacacaa attattacag actgcccgct tgtggctcgc gcgtcataat gggagttttg
       61 atactgtgga ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta
      121 aggatgcctt taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt
      181 aaagcttgct tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat
      241 gccatctccc gtgcagccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc
      301 tgttgtggta atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac
      361 cgtttcgaaa cggagcggcc cagcttacct gccattgcac taaatactga taatgttgtc
      421 ttaacggcga ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg
      481 ctgggtcatg cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatt
      541 gttaaagcag ttgaagccgc cgttacgcgt gatatgacca ttgtggcatt gaccggctat
      601 gacggcggcg aacttgcagg tttgttaggg ccacaggatg tggagatccg cattccttcg
      661 catcgtagtg ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg
      721 atcgataaca cgcttttccc tcaccaggat gattaaggag aatacatgaa ggcattatcg
      781 ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg
      841 ggtaccgctg ctgtgggtac caaagccgca actgacccac gcagtgtcgg tacccaggtg
      901 gacgatggta ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga acagattaag
      961 aaagaaaccc gtattaatgt cacggcgtat cagggcaaag tgctgctggt tgggcagtca
     1021 ccaaatgctg aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac
     1081 gaagtgtata acgagattcg tcagggccag ccgattggtc tgggcgaagc atctaacgat
     1141 acgtggatca ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc
     1201 aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga tggggctggt gactgaacgt
     1261 gaagcgaaag cggcggcaga tattgccagc cgggtgagcg gcgtaaagcg ggtaaccaca
     1321 gcgtttacgt ttattaaata gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct
     1381 tatcaggcct acaacgatgc aaaccgtagg tcggataagg cgtttacgcc gcatccgaca
     1441 tgttttccct caaatcagaa caacagcgcc agcccgccga caatcacacc agacactgcc
     1501 accatcgccc ctgtcagcca taaggctttc gccgggaacg ctttgcgcag cataatcagt
     1561 gacggcaaac tcactgccgg gagcgtcatc aacaatgcca gcgccggagc ggttcccata
     1621 cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat gacaaacaag
     1681 catcctgcta ccgccatcgc caccacccac atcaggctgt tatcgacagc accatcggca
     1741 tgggggaata accagacgcg tgcggcaccc aacaccagta ctgcaaggat gtaaaccggg
     1801 atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc ggctaaaaaa accgctccgt
     1861 gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg cacccatttt
     1921 tgcaccagcg tcgcaatcag caacaccatc accagcccgg ccaccagaca aatcgccgca
     1981 aaaccccagc cgaggacaaa ccccataaac accagcgtcg ccgggtttaa caccggattg
     2041 cccatccaga atgccagcgc accgcccatc gatacctgtt ggcgacgcat ccccgccgcg
     2101 accggagccg cacagcaggt acacatcatg cccggcagcg aaaacagcgt tcccagcagc
     2161 gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg gatcagcacc
     2221 tgaatcaacg aaccgaggat cacccccagc accgccgctt tccatactgc gaggaaatag
     2281 atcatcgcgt aatccaacgc cgcttgccac ggattagcat ccgcctgcgc aaggatagat
     2341 ttaccgatac tgtgggtttc ggctgcagta aacgctttgc cgtagtaatg ttgccatttc
     2401 acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca ccactgaatg
     2461 ggtgttgccg cctgagatga agactgacca gtcatagcat tccctaggaa taagttatga
     2521 taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta ttcagctctt
     2581 ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat gctcaggccg catcgcctct
     2641 gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc tggtaacaaa
     2701 cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc gcccagtaac
     2761 atcgacgggc gagcaatggt caatttcggc cagttctggg caattaatgc ttcttccatc
     2821 tcccctttga cgcggttata gaaaaacggc gagtgggcat tggcccccat agcactgacc
     2881 accagcatat gctgcgcgcc cagccgccgc ccggttaatg cggtatccac taccagcgtg
     2941 taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt gccgagacaa
     3001 caaaacacaa tgtcgatggg ttcggtgacc tgcgccaacg cgtcactcag ttgcggatca
     3061 tggggattaa acaccccggg catatcgccc aacggacgtc gcgtcggcgc agcaatggcg
     3121 ttaactttcg gttcattaat caacatccgc agcaggtgac cgcccaccag ccccgttgcg
     3181 cctgtaatca gtacctgact catcttcgct cctttacaga attgtctgcc ttgcgctcca
     3241 cggctcatgc accagtctta atagaccatg aggtaattat ccccgattgt gggaaattcg
     3301 cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt tttaatcagt
     3361 gatgaatttg aggattcaga atttacttca cccgcagacg agttccgtaa agccggacac
     3421 gaagtaatta ccattgaaaa gcaagcgggt aaaacggtga aaggcaaaaa aggagaagcc
     3481 agcgtgacca tcgataaatc catcgatgaa gtgacgccag cggagtttga tgccctgctg
     3541 ctaccgggcg gccattcacc ggattatctg cgtggggaca accgttttgt cacctttacc
     3601 cgtgattttg tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc gcagctgctg
     3661 atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat cattattgat
     3721 gttaaaaatg cgggcgcgga attttacgat caggaagtcg tggttgataa agatcagctg
     3781 gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc
     3841 ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa
     3901 tttgatttca tcgagccgga tttcccacac cggggccgac agcattctgg caaccggaaa
     3961 gcgatgattg tacgccttgc gcgcgaggtc gctttcctca ccttccagcc tgcggatctc
     4021 acctttaaac tgcacaccgc gaattaacgc taccgttttc ggctgaccgt ttaccgttcc
     4081 ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat
     4141 gtagaaggcg actttctgcg catcaaacag ataaaaagca ttagcgcacc acagttcccc
     4201 ttcctgctgc acacaccagg tgacaacatg ttgttttgcc aaccaacggc tgatggtgat
     4261 gagtgtttcc attgctgttc tcttttatac tgtgggcctg accttaacat gcgacgcaga
     4321 cgatgacacc ctggtttctt tacttgatcc gtaccgccga caataagctt tataccggga
     4381 tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac
     4441 tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc agtgggcgac cgttcgctgg
     4501 cactacgggc ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag
     4561 cggaaggcgc agggtttgca gaactgttga gcagcctgca aaccccggag attaaaagcg
     4621 attgaaatgc tcgtgatact caaccagacc ggttacgcca ttcagcgcgt catccgctaa
     4681 acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc
     4741 cgccagttca aagccgaaac gactgtacag cgccggatcg cccagcgtca ccactgcggc
     4801 atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg ccagcccctg
     4861 tccacggtat ttttcatcaa ccgccaacgg tgccatgccg acccattgca ggtcttcgcc
     4921 ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt catctgtcgc
     4981 caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct tcgcttccgc
     5041 atcactttcg aatgagcgac gcagcagggc atcaataccc ggcgcatcaa tgggaatttc
     5101 tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc ggttttcatt
     5161 cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt cggcatttgc
     5221 tccagttcaa tggcgtccat caggtttttc acatgcagcc ccagctccgt atcgccttca
     5281 atcaccagcc gacgctggaa gaagagcgta tctggatctt gtttacgcgc cgcaatcatc
     5341 agcagatcgc tgacgtcggc actaaaactc acatcagctt gcgcgttctg gctgaccacc
     5401 agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacgcac atgaatactt
     5461 aaccagcggc cttcaagaaa ctccagctcg ccatcatcca gcgcctgacg gaattgccag
     5521 cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca
     5581 ctcaacagag atggccccaa atgcacaata cgggaacgaa gtttatccaa cacgagtttt
     5641 actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat agcggtataa
     5701 atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg ccttaaatca
     5761 aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg cgttttgata
     5821 gcgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct cccggcgctt
     5881 aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga tgataccaat
     5941 gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc ggtgagtttt
     6001 gtccatcaac atcgccgcaa acttcacatc gcgattaaca cttttgcgca tccggacggt
     6061 tacgcccgtt ggcagcgcgc cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc
     6121 ctcgccgacc tcgccatgct ggaatacgcc gccgagcgtt atccgaatat tgagcgccac
     6181 gtatcggtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca tcgccatttt
     6241 gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa acagctggca
     6301 cgggtcacac ctgtaccact ggaagtgttt gctttcggca gcctgtgcat tatgtcggaa
     6361 ggccgttgct atctgtcgtc gtatctgacg ggtgagtcgc ctaacaccgt gggcgcgtgt
     6421 tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc ccgcctgaac
     6481 gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag gttatccgac gctgtgtaaa
     6541 gggcgttatc tggtggacgg cgagcgctat cacgcgctgg aagaaccaac cagtctcaat
     6601 accctggaac tgctgccgga gttaatggcg gcgaatattg cttcggtgaa aattgaaggc
     6661 cgccagcgta gcccggcgta tgtcagccag gtggcgaaag tctggcgtca ggctatcgac
     6721 cgttgtaagg ccgatccgca aagcgcgtgg atggagacgc tcgggtcgat gtccgaaggc
     6781 acgcagacca cccttggcgc gtatcaccgt aaatggcagt gagaagagca atgaaatatt
     6841 ccttagggcc agtgctgtgg tactggccaa aagagacgct ggaagatttt tatcagcagg
     6901 ccgccaccag cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc aagcgccggg
     6961 caaccaaagt tggcgactgg ctggagatgg caaaatcgct cgccggaagt ggcaagcaga
     7021 ttgtgctctc cacgctggcg ctggtgcagg catcctctga actgggcgaa ctgaaacgct
     7081 atgttgagaa cggtgagttt ctgattgaag ccagcgatct cggcgtggtg aatatgtgcg
     7141 ctgaacgcaa actgccgttc gtcgccgggc acgcgctgaa ctgctacaac gcggtgacgc
     7201 tgaaaatatt gctcaaacag ggcatgatgc gctggtgtat gccggtggag ctttcccgcg
     7261 actggctggt gaatctgctt aatcagtgcg atgagctggg cattcgcaac cagtttgaag
     7321 tggaagtcct gagctacggt catctaccgc tggcctactc cgctcgctgc tttaccgcgc
     7381 gttcggaaga ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat ccgaacgggc
     7441 gcaacgtgct gtcgcaggaa aaccaacaag tgtttgtact caatggcatt cagaccatga
     7501 gcggctacgt ttacaacctc ggtaacgagc tgacatccat gcagggattg gttgatgtgg
     7561 tccgcctgtc accgcagggt actgacactt tcgcgatgct cgacgccttc cgcgctaatg
     7621 aaaatggcgc tgcgccacta ttgttgacgg caaacagcga ctgtaacggc tactggcggc
     7681 ggctggcagg actggagctg caagcctaag taaatatgct cgtcatactt caagttgcat
     7741 gtgctgcatc tgcattcgtt caccccagtc acttacttat gtaagctcct ggggattcac
     7801 tctcttgtcg ccttcctgca actcgaatta tttagagcac agctcacttt gttaacaact
     7861 ttaactactc tttaatgcag tattaaagat taatcggtaa caaagtgagc tgttatgact
     7921 gataaaacca ttgcgttttc gttactcgat ctggccccca ttcccgaagg ttcttcagcg
     7981 cgagaagcat tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg cggctatcat
     8041 cgctactggc tggcagaaca ccacaatatg actggcattg ccagtgctgc cacgtcggta
     8101 ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg cggcgtgatg
     8161 ttgcctaacc actcaccgtt ggtcattgcc gaacagttcg gcacgctcaa tacactctat
     8221 ccggggcgaa tcgatttggg gctgggtcgt gcgccaggta gtgaccaacg gacaatgatg
     8281 gcgctgcgtc gccatatgag cggcgatatt gataatttcc cccgcgatgt cgcggagctg
     8341 gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc aggctatggc
     8401 gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gcgcgcaact ggcggcgcag
     8461 cttggtctgc cgttcgcgtt tgcctcacac ttcgcgccgg atatgttgtt ccaggcgctg
     8521 catctttatc gcagcaactt caaaccgtcg gcacgactgg aaaaaccata cgcgatggtg
     8581 tgcatcaata ttatcgccgc cgacagcaac cgcgatgccg aattcctgtt tacctcaatg
     8641 cagcaagcct ttgtgaagct gcgccgcggc gaaaccgggc aactgccgcc accgattcaa
     8701 aatatggatc agttctggtc gccgtccgaa cagtatggtg tgcagcaggc gctgagtatg
     8761 tcgttggtag gtgataaagc gaaagtgcgt catggcttgc agtcgatcct gcgcgaaacc
     8821 gacgccgatg agattatggt caacgggcag attttcgacc accaggcgcg gctgcattcg
     8881 tttgagctgg cgatggatgt taaggaagag ttgttgggat agtgtgtctt aacgcgggaa
     8941 gccttatccg agctggcaac gctgtcctac gtagacctga taagcgaagc gcatcaggca
     9001 ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca ccggcagtaa
     9061 attaaagctc gataaaatat gtaccaatgc gttgccgacg ccaaacacca gaatcagcgt
     9121 aatcatcggc ttgccgcccc agacgcggaa tttcgggctg ccaaagcgtt tacgcgatgc
     9181 gcgggccagc agtgccggaa caattgccgc ccagatggta gccgctaaac cagcataacc
     9241 aatggcgtac aggaatccat tcgggaacag cagaccaccc acaacaggcg gtgcaaaggt
     9301 caacaatgct gttttcaagc ggcccatagc cgagtcatcg aaaccaaaca gatctgccag
     9361 atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa agtttgagaa
     9421 cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg cctgtaccag
     9481 cacatcaata ttaccgccct tctctgcaat accgataaac tccggacgcg ggatgttacc
     9541 catcgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac cgtacaccag
     9601 gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggcttg gcacgttacc
     9661 gtgataacca aacgacgcca ggcagaacgg caatgtcatc agcagatacg gcgcataaga
     9721 tgcattgctt tctgcaacgt tgaacaatgt cgcaggctgc acatgcccca acaggctgcc
     9781 aaaggtgagg aagaaggtaa tgactttcgc ccccagcaca atcgccgtca tgcggctgac
     9841 ggccttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa aacccgccgc
     9901 ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaattg aaccactggc
     9961 agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatac cgttgaccac
    10021 gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac cgattcggta
    10081 attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc aggtaaagat
    10141 cagcgccgcc attgaccaga aaaaccacgc cccggacatg accactggca gagaaaacat
    10201 ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg acggtgacgt
    10261 ttgggtggtg gttagtgttg ccatgagggc ttctctccag tgaaaaatag tgcgactgcg
    10321 ttgttatgca ttgcactgta ccagtacgcg agtacaaaag acagaaaaaa agccccgatg
    10381 gtaaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca gcgatgatta
    10441 cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggag cgcggccttc
    10501 acgacgttcg ccgctaaaac gacgaccatc accacggcca ccttcacggc gttcaccgct
    10561 gaagttacga ccgccttcac gacgttcgcc accgaaacca cgaccaccgc cacgacgctc
    10621 accgccagta tgcggctgtg catcgcccag taactgcatg ttcatcggct tgttgagaat
    10681 gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca gttcgatggt
    10741 ggagtgagaa gcaaacagct tgatgttacc aatgtaacgg ctgctgatgt cgccttcgtt
    10801 agcaatcgca ccaacgatat gacgaacttc aacaccatca tcacggccca cttcaatgcg
    10861 gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt cacggtcacc
    10921 acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac ggaattcacg
    10981 tttcggacgc atcggcgcat ctggcggtac gatcagagta cgttcaccct gtgccatttt
    11041 cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag tcggctgaat
    11101 tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct gctgtacttt
    11161 agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca gttctacttc
    11221 cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct cgcggttctc
    11281 aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga tacggtgaac
    11341 gtaagactca gaatccatcg ggatatcgta gttaactacc aggctgatac gctcaacgtc
    11401 caggccacgg gctgcaacgt cggtcgcaat caggatgtcc agacgaccat ctttcaggcg
    11461 ttccagtgtc tgttcacgca gcgcctggtt catgtcaccg ttcagcgcgg cgctgttgta
    11521 gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac gaacgaagat
    11581 aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt ttttgcgcat
    11641 accccagaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc tggactgaat
    11701 gcgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg cttccggcat
    11761 ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa tggtttcaac
    11821 gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca gaccgctcag
    11881 tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg gagtaccgac
    11941 aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct ggccgccgta
    12001 cagagcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg cttcagcaac
    12061 ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt tcagctcagg
    12121 atcaagattc tgcaacagag gtaaagagaa tgctgcagtt tttccgctcc ccgtctgggc
    12181 catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct gaattggaga
    12241 tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca ggcccagatc
    12301 tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta atggcggcca
    12361 gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt gaaccggctc
    12421 aaagtaggtg tattaacgaa caacaacgcc ctcacccctt aaggtgatgg caatcaaaaa
    12481 agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag gtcatcttgg
    12541 tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa gttatgaacg
    12601 ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccgaatcccc cagacttagg
    12661 tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga ggtgttatcc
    12721 gttgcgtccg ccttgagcct ttccattaac gtttgttcgc taatgttgcc caggtagaac
    12781 tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg tttcaacact
    12841 tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag actacggaaa
    12901 ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa cttgtcacga
    12961 ccgccgtaat ataatgcgat cccgcgattc aagtgcgcgt agttgtaagt tggatcaagc
    13021 tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg cgttaaatat
    13081 atgcctaagt aattgaacac ttcaggcata tccggtcgga ttgccagcgc ttgcgaaaaa
    13141 tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg ctcatataaa
    13201 agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc catacgtgcc
    13261 agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc acttttacgc
    13321 caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca ccagcgcaaa
    13381 aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt tcccggttgc
    13441 taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa atggcaacct
    13501 tactcgccct gttcagcagc cggagcttct ggtgctgcag caggttgaga ctgctcagtc
    13561 gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttccagaac tttcaccggt
    13621 acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc agcgatttga
    13681 gagatgtgga ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc aaagtcaacg
    13741 atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt gatctcttcg
    13801 atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcgatctt cacagtaccg
    13861 tcatcttcga tttcgatggt agtgccagtt tcttcggtca gggcacggat aacagagccg
    13921 cctttaccga taacgtcttt gatcttgtcc gggttgatct tgatggtatg gatacgcggt
    13981 gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac gcccaggata
    14041 tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt ggtgatacct
    14101 tcaattttga tatccatctg caatgcagag ataccctcgc gggaacccgc aactttgaag
    14161 tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac gtagttgtcg
    14221 ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgatcgg cacacctgcg
    14281 tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaggaacc gttggattca
    14341 gtgatttcag acacaacacg tacggtgtac gggaatttgt ccatatccgg catgactgcc
    14401 agcacgctgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg agaaccgacc
    14461 atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa ggtatcggta
    14521 cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cagtacccag cgttgcggta
    14581 accagcgcct gcgtttcacc acgggtgaac agcgcagaac cgtgagtacg cggcagcacg
    14641 ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat acgcggttcg
    14701 cctgccagta cgcggctacg aacaacgttt ttctcgatag cgtgcagaat ttcacccagt
    14761 tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc agatttgatg
    14821 acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc gctcaggcga
    14881 gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc cggctgccag
    14941 tcccaacgtg gtttaccggc ttctttcacc agttcattga tgttctgaat aacaacctgc
    15001 tgttgttcat gaccgaacac tactgcgccc agcatctggt cttcgctcag cagttcagct
    15061 tcagattcaa ccatcagaac agcggcttca gtaccggcaa caaccagatc cagtttgctc
    15121 tctttcagct cgtcctgagt cgggttcagt acgtactggt cattgatgta acctacgcga
    15181 gcagcaccaa tcggaccatt gaacggaata ccagacagag acagtgctgc ggaagcacca
    15241 atcatcgcga cgatatccgg gttaacttgc gggttaacag aaaccacggt ggcgataacc
    15301 tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa tcgggcggtc aatcagacgc
    15361 gcgatcaggg tttcgccttc gcttggacga ccttcacgac ggaagaagct acccgggata
    15421 cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa gtcctgacct
    15481 ggtttggctt ttttctggcc aacaacggta acaaataccg cggtgtcatc catgctaacc
    15541 ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac ggtgtgttgg
    15601 ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct ctttcttaga
    15661 cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc gactaatgac
    15721 aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac ctctgcaacg
    15781 gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt cagcttgaaa
    15841 aaaggggcca ctcaggcccc cttttctgaa actcggcagg aattagcgac gcagacccag
    15901 gcgctcgatg agctgggtgt aacgtgctac gtctttacgt ttcaggtagt cgagcagttt
    15961 acgacgctga gaaaccatgc gcagcagacc acgacggctg tggtgatctt ttttgtgctc
    16021 tgcaaagtgg ccctgcaggt ggttgatctg tgcagtcagc agtgctacct gaacttcggt
    16081 agaaccggtg tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg ttgcttcagt
    16141 acttagagac attttaaaac tccaaagtat atagaatgaa aggacgccga tctctaattc
    16201 agcgatccca gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt aagcggcagt
    16261 attctactcg tagcgacttg ttatcgcaag acggttaaca ttacgccggg tattcaacca
    16321 ccaggcgacg aggcgcgacg cggccttcat cgtcaatttc gcccataccg ataaacttgc
    16381 cgttctcacc ttctgtaacg cgaaccagtc cttccagcgg cgcaccggat gtacgaactg
    16441 ggttaccatt tttgaagtaa acagaagacg ttaacggaag attcaccacc gggtagtccg
    16501 aagctggact gtccattggc atcagtaatg gatcaagtaa ctccgcagcc ggaatatcct
    16561 gctgttcggc ttgttcgacc agttcacgca gatgttccag ggtcaccatc cgttcaaccg
    16621 gatatttact taccgccaga cgacgcaggt aaataacatg cgcgccacaa ccgagttttt
    16681 cacccaggtc atcaatgatg gtgcggatat aagtgccttt tgagcagtga atttccagct
    16741 ccagctcatt gccttcgtgg cgaataaaca gcaattcata aatggtaatc gggcgcgctt
    16801 cacgcggtac ttcaatgccc tgacgcgcat attcgtacag ttttttaccc tgatatttca
    16861 gtgctgaata catcgaaggg atctgttcga tatcgccacg gaaagtatcc agcgccgccg
    16921 ccagttgctc tgcgctaaag gttaccggac gttcttcaac gatttgtccg tcggcatcag
    16981 aagtatcggt acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg gagtccagca
    17041 gatactggga aaacttcgtc gcttccccga agcaaattgg caacatgcca gttgccagcg
    17101 ggtccagcgc accggtatgc ccggcacggt tggcgttata aatccgtttc actttttgca
    17161 gcgcatcatt gctggacata ccctgcggtt tatccagcaa caaaacgccg ttaatgtcgc
    17221 gaccgcgacg acgaggacga ctcattagtc ctccttgctg tcgtccgggt taacacgacg
    17281 ttcttcgtca tgtttgacca cgctggtcac caggtttgac atgcgcatcc cttcaaccag
    17341 agagttgtcg tagaagaagg tcagttccgg cacgatacgc aggcgcatcg ctttccccag
    17401 caggctgcgg atgaaaccag aagcttcttg caacgctttg atgcccgctt taaccgcgtc
    17461 ttcatctttg tcgttgagga acgtcacata tactttggca tacgccaggt cgcgagacat
    17521 ttcgacaccg gaaacggtgg tcatcatgcc caggcgagga tctttaattt cacgctgcag
    17581 gatgagagcg atctcttttt gcatttcctg cgctacgcgc tgcgggcgac caaattcttt
    17641 cgccataata aattctcctg acaaaaaagg gactgttagc ccctttttaa aattaatttc
    17701 aggtggaagg gctgttcacg ttggcctgat aagacgcgcc agcgtcacat caggcaatcc
    17761 atgccggatg cggcgtaaac gccttatccc gcatggaacc ctaaaaacct taagcgatgg
    17821 tacgttggat ctcgatgatt tcgaatactt cgatcacatc gccagtgcgg acgtcgttgt
    17881 agttcttaac gccgatacca cattccatac cgttacggac ttcgttaacg tcatctttga
    17941 agcggcgcag ggactccagc tcgccttcgt agataaccac gttgtcacgc agaacgcgga
    18001 tcgggttgtg acgtttaacc acaccttcgg taaccataca gcctgcgatg gcaccaaatt
    18061 tcggcgattt gaacacgtca cgaacttccg ccagaccgat aatctgctgt ttcagttccg
    18121 gagacagcat accgctcatc gccgctttca cttcgtcaat caggttatag atgacggagt
    18181 agtaacgcag atctaggctt tccgcttcaa tcactttacg tgcagaggca tcagcacgta
    18241 cgttgaagcc aaccaggatg gcgttggacg ccgcagccag ggtggcgtcg gtttcggtga
    18301 taccacctac gccagaaccg atgatcttca ctttaacttc gtcagtagac agtttcagca
    18361 aggagtcgga gatcgcttcc acagaaccct gtacgtctgc cttcaggacg atgttcactt
    18421 cgtgaacttc gccttcggtc atgttggcga acatgttctc gagtttagat ttctgctgac
    18481 gcgccagttt aacttcgcgg aatttaccct gacgatacag tgcaacttca cgcgctttct
    18541 tctcgtcacg tacaacggta acttcatcac ccgcagccgg tacgccggac aggccgagga
    18601 tttccaccgg aatggacgga cccgcttcca gcacttcctg acccagttcg ttacgcatcg
    18661 cacgaacacg accgtattcg aagccacaca gaacgatatc gcccttgtgc agagtacctt
    18721 cacgtaccag aacggtagca accggaccac gacctttatc gaggaaggat tcgataaccg
    18781 caccgctcgc cataccttta cgtaccgctt tcagctccag aacttccgcc tgcagcagga
    18841 tagcgtccag cagttcatcg atgccggtac ccgctttcgc agatacgtgt acgaactggc
    18901 tttcaccgcc ccactcttct ggcaggatgc catactggga gagttcgttc ttaacacgat
    18961 ccggatcggc ttctggttta tcaatcttgt tcactgcaac caccaccggt acctgcgccg
    19021 ctttcgcgtg ctggattgct tcgatggtct gcggcatcac gccgtcgtcg gcagcaacaa
    19081 ccaggactac gatgtccgtt gcctgcgcac cacgagcacg cattgaagta aacgcggcgt
    19141 gccctggggt gtccaggaag gtgatcatgc cgttttcagt ttcaacgtgg tatgcaccaa
    19201 tgtgctgggt aatgccgccc gcttcgccag aggccacttt cgttgaacga atgtagtcca
    19261 gcagagaggt tttaccgtgg tcaacgtgac ccatgatggt cacaaccggc gcgcgcggtt
    19321 cagccgcagc ccccgtgtca cggtcgctca ttaccgcctc ttccagctcg ttttcacgac
    19381 gcaggataac tttgtggccc atctcttcag caaccagctg tgcggtttcc tgatcgataa
    19441 cctggttgat ggttgccatt gcgcccagtt tcatcatcgc tttgatgacc tgagagcctt
    19501 taaccgccat cttgttcgcc agttcgccaa cggtgatggt ttcgccgatc acaacgtcac
    19561 ggttaacggc ctgagctggc ttctggaagc cttgctgcag ggaagaacct ttacgttttc
    19621 cgcctttacc tccacgtact gctgcgcgag cttcttcacg atcagctttt gattcagcgt
    19681 gtttgttgcc tttcttcgga cgcgctgctt tcgcgttacg accacggcca cggccgcctt
    19741 cgacttcacg atcgctttcg tcttctgcct ggcgagcatg ttgagaagta gtgacgtgat
    19801 aatcgctgga atcttcagtc ggttccgcgt tatcagtcca tttgttttct tccgccatac
    19861 gacgtgcttc ttcagcaacg cgacgtgctt cttcttcgag tttacgacgc gcttcttctt
    19921 cagctttacg cttgagctct gcagcttcct gctcacggcg ggctttttca gcctgggcgt
    19981 ttttagtcat atcgtcttgt tgattgctca ctttgtcttt ttccgcagct tcacgtttcg
    20041 cttgttcagc agcttcacgc ttagcttgtt ctgcggcctc acgttcagct ttttgttgcg
    20101 cctcgcgttt agccgattct tctgcctcac gacgggcttg ctcttccgct gcaaggcgtt
    20161 cagcctcttg cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg
    20221 atttgctttt tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg
    20281 tcaatttgtc cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt
    20341 gtgcggacac agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca
    20401 ggcgttccac ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc
    20461 tgttccttcc tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag
    20521 ccataatcag tgctccggct ttttcgtcgg tcaccccttc gatatcagcc agatcatcaa
    20581 tgccctgttc ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca
    20641 aatcacgatc tatcccttca aggttcagca gatcgtcagc cggtttgtta tcaccgaggc
    20701 tttcttcctg ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt
    20761 caacggtcgg ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca
    20821 gttcttccag cgtcgagaag ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt
    20881 cgagatattt ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca
    20941 ggtcgtcaac ggtcatcacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt
    21001 tctgaccgtt acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg
    21061 tgtgtttatc ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca
    21121 caaactgcgc cgggttatca tcccacagga cgatatcgat acgctcgccg cccagttcag
    21181 tagaaaccgc ctgaacacgc gcgccacgca tacctacaca ggcacctacc ggatcgatac
    21241 gtttatcgtt ggttttcacc gcgattttcg cacgagaacc cggatcgcga gccgctgctt
    21301 taatttcaat cacttcttcg ccgatttctg gcacttcaat acggaacagt tcgatcagca
    21361 tttccggctt ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg cgaacggaat
    21421 agagcacgcc acgaacgcgg tcgccagggc ggaagttttc acgcggcagc atatcttcgc
    21481 gcaggatcac ggcttcagca ttgttgccca gctccagaga gatgttgtcg cggtttactt
    21541 ttttcaccac gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg
    21601 cacgttcggc ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtagtgatac
    21661 ggtcaaaggt aacagactca atctgatctt caacgtaatc gcccaggttc aggctttcat
    21721 cttcataacg tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa
    21781 ctaaccagcg acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acgcggacgt
    21841 cgatctcttg ttcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga
    21901 aaatcttctc gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt
    21961 ctttgttcat cgcgggcttt tcacctcatc cagactatta aaagtgggga accaagttcg
    22021 ccttctggat attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca
    22081 tttcaccgtc taccgctttg ataacgccct gccatttacg acggttttgt accgccatac
    22141 ggagaaccag agtcacctct tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg
    22201 ggcgatcgag acccggtgag gagacttcca ggttataagc aacggtgatg ggatcttcaa
    22261 catccagcac agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat
    22321 cttcactatc aatatagatg cgcagtgtgg atgtgtgacc gcgaataaat tcgatgccaa
    22381 ccagttcaaa acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta
    22441 atgtggacaa gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag
    22501 tcagataaca aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa
    22561 ctgcggtctg gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc
    22621 ttcacagtat atttgaaaaa ggactctaag ggaaa
//
LOCUS       DADLTD010000049        22583 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLTD010000049 DADLTD010000000
VERSION     DADLTD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.049"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..257)
                     /locus_tag="JN701_001506"
     CDS             complement(<1..257)
                     /locus_tag="JN701_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7364000.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDC"
     gene            complement(248..718)
                     /locus_tag="JN701_001507"
     CDS             complement(248..718)
                     /locus_tag="JN701_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7364001.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(903..1697)
                     /gene="thyA"
                     /locus_tag="JN701_001508"
     CDS             complement(903..1697)
                     /gene="thyA"
                     /locus_tag="JN701_001508"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY7364002.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="JN701_001509"
     CDS             complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="JN701_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY7364003.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="JN701_001510"
     CDS             complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="JN701_001510"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7364004.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="JN701_001511"
     CDS             complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="JN701_001511"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY7364005.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            6204..6893
                     /gene="mutH"
                     /locus_tag="JN701_001512"
     CDS             6204..6893
                     /gene="mutH"
                     /locus_tag="JN701_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY7364006.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6962..7675
                     /locus_tag="JN701_001513"
     CDS             6962..7675
                     /locus_tag="JN701_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7364007.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7813..8031
                     /gene="ygdR"
                     /locus_tag="JN701_001514"
     CDS             7813..8031
                     /gene="ygdR"
                     /locus_tag="JN701_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY7364008.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8139..9179
                     /locus_tag="JN701_001515"
     CDS             8139..9179
                     /locus_tag="JN701_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY7364009.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPSIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="JN701_001516"
     CDS             complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="JN701_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY7364010.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNSTPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(10397..12556)
                     /gene="aas"
                     /locus_tag="JN701_001517"
     CDS             complement(10397..12556)
                     /gene="aas"
                     /locus_tag="JN701_001517"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY7364011.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            12945..13976
                     /gene="galR"
                     /locus_tag="JN701_001518"
     CDS             12945..13976
                     /gene="galR"
                     /locus_tag="JN701_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY7364012.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="JN701_001519"
     CDS             complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="JN701_001519"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY7364013.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            15367..16302
                     /gene="lysR"
                     /locus_tag="JN701_001520"
     CDS             15367..16302
                     /gene="lysR"
                     /locus_tag="JN701_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY7364014.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="JN701_001521"
     CDS             complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="JN701_001521"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7364015.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(17110..18525)
                     /locus_tag="JN701_001522"
                     /pseudo
     CDS             complement(17110..18525)
                     /locus_tag="JN701_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="JN701_001523"
     CDS             complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="JN701_001523"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY7364016.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="JN701_001524"
     CDS             complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="JN701_001524"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY7364017.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(20754..21935)
                     /locus_tag="JN701_001525"
     CDS             complement(20754..21935)
                     /locus_tag="JN701_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY7364018.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            22190..>22583
                     /locus_tag="JN701_001526"
     CDS             22190..>22583
                     /locus_tag="JN701_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364019.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVS"
BASE COUNT         5791 a         6047 c         5440 g         5305 t
ORIGIN      
        1 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc atgacaataa
       61 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
      121 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
      181 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
      241 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
      301 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
      361 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
      421 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
      481 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
      541 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
      601 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
      661 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
      721 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
      781 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggtg tctcgaagaa
      841 tttaatggag ggtaaaaaaa accgacgcac actagcgtcg gctctggcag gatgtttcgt
      901 aattagatag ccaccggcgc tttaatgccc ggatgcggag cgtagccttc aatctcaaaa
      961 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
     1021 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
     1081 gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg cgccatcata
     1141 tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag
     1201 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaacgcatgg
     1261 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
     1321 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
     1381 ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
     1441 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
     1501 tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca acgtttagtt
     1561 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
     1621 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
     1681 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
     1741 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg tcccatgctg
     1801 atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
     1861 atgcgaaacg cgccgtaacc aatcaggaac aaacctgaga cagctcccat tgggcgcggt
     1921 ttacgaatat acaggttgag gataataaac agcactacac cttccagcaa cagctcgtaa
     1981 agctgtgatg ggtggcgcgg cagcacaccg taagtatcga aaatggattg ccactgcggg
     2041 ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat ggcaaacggg
     2101 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
     2161 ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta
     2221 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
     2281 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaagttg
     2341 tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc cgcatagagt
     2401 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc
     2461 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
     2521 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
     2581 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
     2641 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
     2701 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
     2761 ccgcgacgct ccataaaggc tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc
     2821 agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt
     2881 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
     2941 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat accatgtatt
     3001 tccgcttccc gggcgatcat tgccagagct cgtaacattg caggatgaag gctgtcataa
     3061 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
     3121 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
     3181 ggtacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
     3241 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg
     3301 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
     3361 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
     3421 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
     3481 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
     3541 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
     3601 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
     3661 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
     3721 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtatc
     3781 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
     3841 agcaccgaag gctgaatatc cgcgcccatc acggtaggga gccccagcgc acgtaccatg
     3901 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
     3961 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
     4021 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
     4081 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
     4141 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
     4201 aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc
     4261 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
     4321 gcttcttcca gcgccccggt caatcgttcg cgttccagag ccggatccag cgttgatgcc
     4381 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
     4441 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
     4501 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
     4561 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
     4621 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
     4681 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
     4741 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
     4801 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
     4861 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
     4921 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
     4981 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
     5041 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
     5101 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
     5161 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
     5221 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
     5281 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
     5341 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
     5401 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
     5461 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
     5521 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
     5581 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
     5641 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
     5701 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
     5761 taggcatttt aaaatattca ttataagtca atgtattaca tgtaatttga gtaatgaaaa
     5821 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
     5881 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
     5941 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
     6001 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
     6061 cctattgcag tgctgcgcaa ctcgattatc ggcagcctaa aaagcaatat aaatcagaga
     6121 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggtacataa ttgctgtttg
     6181 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
     6241 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
     6301 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
     6361 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
     6421 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
     6481 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccacg
     6541 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc
     6601 tggcgcagcg tcgcgtagga tccccgttgc tgtggacccc gaatgaagag gaagaccgac
     6661 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
     6721 tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc
     6781 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
     6841 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
     6901 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
     6961 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
     7021 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
     7081 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
     7141 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
     7201 attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat
     7261 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
     7321 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatctttag
     7381 cctcgactcg gtgattacgg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc
     7441 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
     7501 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
     7561 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
     7621 ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc
     7681 cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc
     7741 ctgctttcaa gatatataac gtcgttttat aaacagacta ttatcatagg taggccaaat
     7801 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
     7861 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
     7921 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
     7981 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
     8041 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
     8101 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
     8161 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
     8221 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg
     8281 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
     8341 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
     8401 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
     8461 agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcgatc
     8521 tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct
     8581 ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
     8641 aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc
     8701 gctacctgca tctggcggac aaacacgatc tgccgagtat tgtcaccatt cagaaccctt
     8761 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
     8821 tcgaactgct ggcctattca tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg
     8881 gtgcaaaaac cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
     8941 agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggcctggacc
     9001 ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
     9061 gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg
     9121 aagacgtatt agctgaaatt gaagcggtgc atcaggttta tacttatccg gcaccataag
     9181 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
     9241 cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
     9301 taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
     9361 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
     9421 cgcattgagc ggaacgacaa aaaagccccc catcacgcca atcagcatca acaaggcata
     9481 ggctggcagc agctcgtgtt gcagggaaaa aatcagtacc accacgccaa tcaaaatccc
     9541 ggctggcata cagcgtgaca cggtttccag cgtaactaac ttcgctgccg cacctgcgcc
     9601 aaccacgata ccaatcgcta ccatcgcgtt gagataggtg ggcgtagagt tatcggtaat
     9661 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca
     9721 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca
     9781 ggcattcagg aaactgcggg tcatgttgat gagattccaa gactgccccg gacgcgccgc
     9841 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
     9901 ggccagggcg acgaggacat gccagtcagc cagcacacca ccggctacgg aaccgagcaa
     9961 aatcgccgct atggtagaag cttccattaa accgttagct ttcactaact tactacccgt
    10021 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacccccac
    10081 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    10141 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    10201 aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    10261 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtaggg cattatcgcc
    10321 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    10381 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    10441 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
    10501 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgagcg
    10561 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    10621 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    10681 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    10741 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
    10801 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
    10861 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg
    10921 acaacaggcg cgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
    10981 tcggtacgtt gatagaaacg acaggcgcgc actcggttac accatagcct tcaaggatgc
    11041 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    11101 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
    11161 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaac tccggcacaa
    11221 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
    11281 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
    11341 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
    11401 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    11461 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
    11521 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
    11581 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    11641 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttacccca gcagtgtagt
    11701 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa aatcactgcc gcactgatgc
    11761 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
    11821 gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta aagttgacgt
    11881 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
    11941 gagtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
    12001 cagcgatttt gcgacggtca cgggcacgcg acgcatccgg catcgccacc tgcgttggag
    12061 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
    12121 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
    12181 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc
    12241 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatag
    12301 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
    12361 accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc
    12421 caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagaacg cgctcgccct
    12481 tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
    12541 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
    12601 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
    12661 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
    12721 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
    12781 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
    12841 cgtttggctc acattcccac gatgaaaaca cgccactcct tgaaccaacg ggcgttttcc
    12901 gtaacactga aagaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
    12961 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    13021 ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
    13081 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
    13141 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    13201 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
    13261 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    13321 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    13381 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    13441 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    13501 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    13561 gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    13621 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    13681 attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    13741 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
    13801 ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg gctttggcgc
    13861 tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc
    13921 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    13981 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    14041 gcctgaccat tatcaaacag aacttctggt aacagcgaac ggctattgta gttggatgac
    14101 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc
    14161 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    14221 cataacggtc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    14281 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    14341 aacccggcat caaccagcac aaagtggcgg ctccccattt gtttgacgct ccgcacctga
    14401 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
    14461 gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc ataataatgt
    14521 tcggtatcaa ccgcctcttc accctgttga taaggaacag aaagcccacc gcccgcagaa
    14581 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgt
    14641 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaga
    14701 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    14761 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
    14821 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    14881 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    14941 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    15001 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
    15061 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc
    15121 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    15181 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    15241 ggcataacaa actccagata agtgcttttt tatgattacg ccacatcata aaaagaataa
    15301 aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga
    15361 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
    15421 ggaagcctga ctgaggcggc acacctgcta cacacctcac agccaaccgt cagccgcgaa
    15481 cttgcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tgggcgatta
    15541 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
    15601 gatcgaattg tcagcgctgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
    15661 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
    15721 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
    15781 ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc tgcgggaaca
    15841 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
    15901 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat cagcctttcc
    15961 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
    16021 cgcatgatcg tagaaaccca cagcgccgcg tcaatctgcg caatggtacg ggcgggggta
    16081 ggtgtttcgg tggttaaccc gctcaccgca ctggattatg cggcaagcgg tttagtggtg
    16141 cggcggttca gtattgcggt tccgttcaca gtcagcctga tccgccccct gcaccgcccg
    16201 tcatcagcgc tggtgcaggc gtttagtgag catttacaag cggggttacc gaaactggtc
    16261 acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg
    16321 catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctggc accagtaaac
    16381 caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca
    16441 cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa
    16501 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
    16561 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
    16621 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga
    16681 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
    16741 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
    16801 cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
    16861 gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
    16921 aacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca
    16981 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
    17041 caacggccaa gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
    17101 cccgtgaaat cagatgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
    17161 atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtgatgc ccacaaacgc
    17221 aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctgt cgagcagcgt
    17281 caggaaggtc gccgataatc atattcgaca cccagttcgt ggtggtcgaa caggtaatac
    17341 cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg
    17401 cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaatc
    17461 cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta
    17521 acgccatcac gctgaaacca attttcagag ccggtttacc cctgctttat ctaccgtaaa
    17581 caccgcaata aaggtggcga acataaaggt cagccctacg accagagtcg caatcatctg
    17641 ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcgtagt acatgatgat
    17701 gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca cagcacgacg
    17761 gacgttacgg ttgatcttaa acagtgccca accgccctgt tttaacttca ggctttcacg
    17821 aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac gcaatacttc
    17881 ttccgcctca atatgacgcc ccttttccgc caaccagcgc gggctatttg gcaggaagac
    17941 caccagaata atcagcagaa ctgctggtaa agcaagaacc cccaacattg cgcgccagtt
    18001 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    18061 caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt cagaaagata
    18121 cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agccccacac gagcggcgat
    18181 taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa acaggatggc
    18241 ccccgccatc aggctgtatt tacgccccag gcggaacgac agccaaccat taaacagcgc
    18301 accaattgct gcgccgagca tcatgctact aaccacccat tcctgcaaat gactggtcag
    18361 cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat caagaccaaa
    18421 taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc gcgtatcccg
    18481 caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt cctgccagca
    18541 gagagtaaga catagtgaaa aaatacgtga acaactcacg caggtgtcag gtcggaaaca
    18601 gcataaatat ggattaaatt gctgcgacat gtcgttatgt gatggatatt ccaattttca
    18661 aattaagttg aattattgag attattatta accacctaat tttacagcag ataaaattca
    18721 taaagttcat taattgataa ttaatatgga ttatttcata accatgatat ggattatgat
    18781 gatctacagg tataaaaaac cctgccatgc ggcagggtca taaaagtaag aagaatgaat
    18841 taacgcgcca gccaaccgcc atccacggca atggtataac cattcacata atctgaagcg
    18901 ctggaggcaa ggaacactat cggccccatc aggtcactcg gcagtcccca acgaccagct
    18961 ggaatgcggt cgagaatttc cgcgctacgt tgttcatctg cccgtagttg ttgagtattg
    19021 ttggtcgcca tgtaacccgg agctatcgca ttaacattaa tgttgtgttt agcccattcg
    19081 ttcgccatca atcgcgtcac acccatcacg ccgctttttg atgcggtata agaaggcaca
    19141 cggatcccgc cctggaagga gagcattgac gcgatattga taatcttgcc gccattgcct
    19201 tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga atacgctctt gatattcagg
    19261 ttcatgacat cgtcccagtc tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat
    19321 ccggcgttat tcaccaggat atcaatatga ccaaattcag ctaccgcgcg atccagcagt
    19381 ccaggaatgc catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc
    19441 gccgtgacct gctcgatggt ttcagtcggt tcaacgatgt taatgccaac aatgtcacag
    19501 cccgcttgcg ccagccccaa cgccatcccc tggcccagcc cagtatcaca accagtgacg
    19561 accgcaactt taccttcgag agaaaatgca cttaaaatca taacaatacc ttattcgttt
    19621 atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt
    19681 tttcaccaac catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg
    19741 accagctcgg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg
    19801 gttgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt
    19861 ccatccggcg ctcgtgggtg tgacacggca tggtgttcca caagttacct ggagccagct
    19921 ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa
    19981 tagtgcgacg gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccgggg
    20041 tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg
    20101 gagtggcggt atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg
    20161 cgtcgcggtg accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa
    20221 tattgataac acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa
    20281 cttccccgcc aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt
    20341 caatgtggct gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt
    20401 cattgtgcag cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta
    20461 cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac
    20521 tgtttcgttt tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat
    20581 aaaacaatgt tttattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat
    20641 gtcatgagaa tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat
    20701 tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg
    20761 tcacgttcaa tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct
    20821 ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa
    20881 gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc
    20941 cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata
    21001 agatcgacat cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc
    21061 ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc
    21121 agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg
    21181 ccatcgttta tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga
    21241 tttaaacggg ctaaaccttc tgcactggcg tcagtgcatg gctgttcatc ggtatcaaca
    21301 accaacgtct gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg
    21361 gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga
    21421 cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg
    21481 aaggcatccc acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt
    21541 gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg
    21601 gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta
    21661 agcccagaac cgcaaacgtc attaatagtg attgcagaaa cgctattagg aagaccacct
    21721 ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga
    21781 attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc
    21841 aaactaccaa gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt
    21901 gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg
    21961 caatagtaaa tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc
    22021 acaaaagaaa tatgcctgag cagcagtcag agacataact ggcacgtaag gtttgcaacc
    22081 actaacccac caatagaggg gtagataggg cgttaatctc ccatacttaa cctggtttat
    22141 ggtaaattgc cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat
    22201 ttggtctaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg
    22261 cacgctcttc cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc
    22321 tctggtcgcc tggcctttaa catattggcc acataaagct ttatgccagt tcatcctctc
    22381 atcgaaaaca tcagcaggtg aagggataac gggcgcggta acacactact atggcaagaa
    22441 gattggtaat ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata
    22501 tgcagtggca attaccaact cacttacaga acagctggca aagcatatgg ttattgatct
    22561 tcgcatccgt atgttggtga gtc
//
LOCUS       DADLTD010000050        22415 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLTD010000050 DADLTD010000000
VERSION     DADLTD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.050"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..745)
                     /locus_tag="JN701_001527"
     CDS             complement(<1..745)
                     /locus_tag="JN701_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7364020.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISS"
     gene            963..2615
                     /locus_tag="JN701_001528"
     CDS             963..2615
                     /locus_tag="JN701_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7364021.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="JN701_001529"
     CDS             complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="JN701_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY7364022.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3335..4129)
                     /locus_tag="JN701_001530"
     CDS             complement(3335..4129)
                     /locus_tag="JN701_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364023.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4267..4608
                     /locus_tag="JN701_001531"
     CDS             4267..4608
                     /locus_tag="JN701_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7364024.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4823..7327)
                     /gene="copA"
                     /locus_tag="JN701_001532"
     CDS             complement(4823..7327)
                     /gene="copA"
                     /locus_tag="JN701_001532"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY7364025.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARINLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7589..8521
                     /gene="glsA"
                     /locus_tag="JN701_001533"
     CDS             7589..8521
                     /gene="glsA"
                     /locus_tag="JN701_001533"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY7364026.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8524..9816
                     /locus_tag="JN701_001534"
     CDS             8524..9816
                     /locus_tag="JN701_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7364027.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYSYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9941..10348
                     /locus_tag="JN701_001535"
     CDS             9941..10348
                     /locus_tag="JN701_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY7364028.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10349..10807)
                     /locus_tag="JN701_001536"
     CDS             complement(10349..10807)
                     /locus_tag="JN701_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY7364029.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10804..11721)
                     /locus_tag="JN701_001537"
     CDS             complement(10804..11721)
                     /locus_tag="JN701_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7364030.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11867..12544
                     /gene="fetA"
                     /locus_tag="JN701_001538"
     CDS             11867..12544
                     /gene="fetA"
                     /locus_tag="JN701_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY7364031.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12531..13310
                     /gene="fetB"
                     /locus_tag="JN701_001539"
     CDS             12531..13310
                     /gene="fetB"
                     /locus_tag="JN701_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY7364032.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="JN701_001540"
     CDS             complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="JN701_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY7364033.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="JN701_001541"
     CDS             complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="JN701_001541"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7364034.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="JN701_001542"
     CDS             complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="JN701_001542"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAY7364035.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15681..16367
                     /locus_tag="JN701_001543"
     CDS             15681..16367
                     /locus_tag="JN701_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7364036.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16364..18376
                     /locus_tag="JN701_001544"
     CDS             16364..18376
                     /locus_tag="JN701_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7364037.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSMVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLCRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRCDLLDRWQQLPPESPNYFLINIATEQVAPLKAFL
                     AEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNWP
                     PKADEVSMEEGLAKRLNVALGGTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFPE
                     GALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVG"
     gene            18377..18775
                     /locus_tag="JN701_001545"
     CDS             18377..18775
                     /locus_tag="JN701_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAY7364038.1"
                     /translation="MLEQVSRALEVMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRT
                     LGAGKKLLRTTLWCEFAMLGFVSGLVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLP
                     CSGALLLSLFGGWLGARLVKGKALFRQFAG"
     gene            19204..22415
                     /locus_tag="JN701_001546"
                     /pseudo
     CDS             19204..22415
                     /locus_tag="JN701_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014860.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5160 a         5887 c         6308 g         5060 t
ORIGIN      
        1 agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac agaatgctga
       61 ctgccagtac cactttgttg cgcggcagtg gattgattat cgtcgctttg ggttttcctt
      121 tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc acgatcgcca
      181 gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg ataatcaccg
      241 ccgtcagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag atagattgcg
      301 ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc ggatgaaaga
      361 ccgatgaact ggtaccgacc agcgccgccg ccagcagaac tgcgccaaca ctgcccgcca
      421 gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac gaccacggca
      481 tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag gcgagctgga
      541 aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac tgaagcagag
      601 gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag aggctgatag
      661 cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg cccgccacag
      721 gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc gatgtaaaga
      781 atgatcttat ttgtgattat tacccgacta acatacctgt aagcgtcgcc tgaaggaagt
      841 ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc ggacgcagac
      901 ttgtctatta tgttgcgtca tactattttt caacacgttg aaatcaggtc agggagagaa
      961 gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt acactggcga
     1021 gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt ctgcatacca
     1081 atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg gcggcgcaaa
     1141 aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc gtgctgctac
     1201 tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat gccgaacctg
     1261 attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat catgaatttg
     1321 ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg ttgctttccg
     1381 cgaatatcta ccagaaaagt accggcgagc gcctgtttaa accatgggcg ctgtttaagc
     1441 gtcaggatct gaaaattgcc gtcattgggc tgacaaccga tgacacagca aaaattggta
     1501 acccggaata cttcactgat gtcgaatttc gtaagcccgc cgatgaagcg aagctggtga
     1561 ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc catatggggc
     1621 attacgataa tggtgagcac ggctctaacg caccgggcga tgtggagatg gcacgcgcgc
     1681 tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagctccg gtctgcatgg
     1741 cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa ccagatcaac
     1801 aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga cgggctgatt
     1861 ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg gtgaacctga
     1921 agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact cctgaaatcg
     1981 ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca gaacaaaggc aaagcgcagt
     2041 tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac aaagtgcgtt
     2101 ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc actggtgccg
     2161 actttgcggt gatgagtgga ggaggaattc gtgattctat cgaagcaggc gatatcagct
     2221 ataaaaacgt actgaaagtg cagccattcg gcaatgtggt ggtgtatgcc gacatgaccg
     2281 gtaaagaggt gattgattac ctgaccgccg tcgcacaaat gaagccagat tcaggtgcct
     2341 acccgcaatt tgccaacgtt agctttgtga cgaaagacgg caaactcaac gacctgaaaa
     2401 tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta aacttcaatg
     2461 ccaccggcgg tgatggctat ccgcgccttg ataacaaacc gggctatgtg aataccggct
     2521 ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg gatgtgagtg
     2581 tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga tatttgcatc
     2641 cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag gatcttcgcc
     2701 agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccagaaac ataaatagtg
     2761 gcaaattctt gtgcgggggc gtcgataatc gtcggcagac gttttttctg ccccagtggg
     2821 ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg ccatcggatc ggccatctca
     2881 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
     2941 gtaacggcca ctgccagatg tttcatatca ccgttcactg ccaccagtag cgttttgtag
     3001 acttgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagcc
     3061 ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag taatttaact
     3121 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
     3181 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
     3241 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
     3301 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc ggcaaattcc
     3361 cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc ggcatggcgc
     3421 gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac agcgactgac
     3481 tgaacgtatt gggcagcgtt atatcattat tttgcggagg tgcattcagc caccagctca
     3541 tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg tccagcagcg
     3601 ccagtccttt gtcagggagc tggagcaaca tggtaatctg gttttcagcc ccttccagtt
     3661 caatcacggg tttatgttgt tgcttcgccg cctgcaataa ctggtaatcg ataccgtatt
     3721 ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg atttgccaca
     3781 gcggttgagt agaaaagagc gacggagaaa tgcccatctc ctggctaatg tgctgcagat
     3841 tttggagttg ctcctcgtta atgcgctctt ccagcgcctc gcaagtaggc aaattagcaa
     3901 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
     3961 ttttgagcaa acgggtgggc aggggagtca tatcgtggct acccatatga atactgccaa
     4021 tcagatgaaa atggcgattg ccgggaaggg tgatatcgat ggcaggccag gtgtaatgat
     4081 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
     4141 ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg gggacttgtt
     4201 taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa gcaacagtta
     4261 aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct tctctatgaa
     4321 tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca tgttcatcgt
     4381 aatagcgtca gtacactgat caataataat cgtaaactca ccactgagat ggcattccgt
     4441 ctggcgaaag tttttgatac cacagtcgat ttttggctaa acctccaggc ggcagttgat
     4501 ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat tgaaacagtg
     4561 gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg ccaaaacatc
     4621 gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga gcaccattgc
     4681 ctgatgcgac gcttacccgt cttatcaggc ctacaaacct gtgcctgaac cgtaggccgg
     4741 ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc cgccctaaag
     4801 cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac cggttggcgt
     4861 tactcactac ggtaatcgac gagagcgcca ttgccgctcc ggcaactacc gggttaagca
     4921 gtgttccagt aaacggccac aaaataccag cggcgaccgg aataccaatg ctgttgtaga
     4981 taaacgcacc gagcaggttc tgcttcatat tgcgcagcgt tgcgcgggaa atcgcgagcg
     5041 catctgcaac acccatcagg ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa
     5101 catcactgcc gccacccatc gcaataccga catccgcctg agccagcgct ggcgcgtcgt
     5161 taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt ttgatcgctt
     5221 cggctttacc gtctggcagc accccggcga ttacctcatc aatccctgct tctttggcga
     5281 tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat cccgctttat
     5341 gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca gccagcaggg
     5401 ctactacttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat gcctgagctg
     5461 aaatatcggc ttcaatcgct ttagtatcga cttgctggtc atttaacagc gccggattac
     5521 ccagcaataa cgcatgacct tcagcttcac cgcttacgcc cagcccgcgc aatgtgcgga
     5581 aaccgttgac ctgcggcagc tgcatatcgc ctgctttatc gaggatcgct cgtgccagcg
     5641 gatggctgga accttgctcc agcgccgccg ccagacgcaa tgcttgcgct tcatcaacat
     5701 cagcaaatgt ttttactgcg acaacctgcg gcttcccttc ggtcagcgtc ccggttttat
     5761 cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca
     5821 cgccaaactc agccgcccgc ccgacgccgg aaataatcga cattggcgtc gccagcccca
     5881 gcgcacacgg acaggcaata atcagcaccg tggtggctat caccagggta tagacaatct
     5941 gcggtgccgg accaaagaaa taccagattg ctgcactgat cagcgcaatc accactacta
     6001 ccggcacaaa tacggctgag attttatccg ccagctgacc gatttctggc ttgctgctct
     6061 gggcctggcg caccatgcga ataatccttg acagcgtggt atggctgcca accgcactgg
     6121 cgcgaaatag cacgctaccg tcctgtacca ctgtcccggc atggacgctt tcgccttcgc
     6181 ctttttgctg cgggattggt tcgcccgtca gcatcgcttc atccagccat gcttcgccct
     6241 gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagt aacatccctg
     6301 gctgaacttc cgccagcggc acgctttttt caccctctgc ggtgaccact cgcgccgtcg
     6361 gtggggttaa atcgagtaat ttctccagcg ccttagaaga acgctgacgc gcgcgcgctt
     6421 ccagcatatg gccgagatta atcagaccga taatcatcgc actggcttca tagtaaagat
     6481 gccgcgcttc catcgggaac cactgcggcc acaggttgac gctcatcgaa tagagccacg
     6541 ccacacccgt acccagcgcc accagcgtat ccatcgtcgc cgcaccgttc agcaggcttt
     6601 tccacgcgct gcggtaaaaa tgcccgccgg cgaaaaccat taccgccagg gttatcaggc
     6661 cgataactaa ccacaggctg cggttgtcag cggtgaccat catgttatcg ccgatcatcc
     6721 cccagaccat taccggaata cccaccgcca gtgcgacaat cgcctgccag cggaagcgct
     6781 tcatcgtagc gacggcggtt tcctgctggc gctcgcggcg tttcgcgtca tcttcaatcg
     6841 cttccgcgcc gtagcccgct ttttccaccg cctgcactaa atcttgtggg gaggcactgc
     6901 ccatcaccag cgcagtacgc tccgccaggt ttatccgtgc ctgagtgacg cccggtacgc
     6961 tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg ctcagcagca
     7021 actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa accgctgtca
     7081 gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg cttacagatg
     7141 cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca gtcccggtaa
     7201 cgtgcgcttc agtgatagac acatccgctt gttccacatc cgggcgctgt tcgagacttt
     7261 ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc cagggtcagg tcgatagttt
     7321 gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt taaaccttcc
     7381 agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta aagattaaaa
     7441 aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg tagttaagtt
     7501 gctggtgcta agttaaatca ggatgcctga aaatcggcac cggggtgagg aattacctcc
     7561 cgcatctata aaaaggagtt aacaaaagat gttagatgca aacaaattac agcaggcagt
     7621 ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg attacattcc
     7681 ctttctggcg aatgtaccag gtcaactggc ggtagtggct atcgtgacct gcgatggcaa
     7741 cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct cgaaagtctg
     7801 tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca aaattggcgc
     7861 tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg gcggcaaacc
     7921 gctttcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta acgctgaaaa
     7981 tattgaacaa cgctggcagc gaattttaca tatccaacag caactggctg gtgagcaggt
     8041 agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata accgggccat
     8101 agcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag cctgtgacgt
     8161 gtacacccgt cagtgctcca cgctcatcaa tactgttgaa ctggcaacgc ttggcgcgac
     8221 gctggcggcg ggtggtctga atccgctgac gcataaacgc gttcttcagg ccgacaacgt
     8281 gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg gtgactgggc
     8341 gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc
     8401 tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggta acagtgttcg
     8461 cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt ttaagggctg
     8521 atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg gaatgtcgtt
     8581 tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg gcaggctgcg
     8641 ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt ggcgatgttt
     8701 tccggttatt cctatgcgcg tctgggggcg agctatccca gcaatggcgg cattatcgac
     8761 ttctttcgtc gcggattagg caacggcgtc ttttcgctgg cgctctcgtt actgtacctg
     8821 ttgacgctgg cggtgagcat cgccatggtc gcccatgctt ttggcgctta tgccgtgcag
     8881 tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt ggggagcatt
     8941 gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct ggaagtgatc
     9001 ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt ctggtcgctg
     9061 caaccagcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt ctcctgtatt
     9121 gggataactt tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc ggataaagtg
     9181 aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt taccacgttg
     9241 ctttatatct cgctggcact ggttttgctt agcgatgtat cggcattaga gttagaaaaa
     9301 tatgccgata ccgccgtagc gcaggctgct tctccgctgc tcggacatgt gggttatgtg
     9361 atcgtcgtca tcggcgcttt actggcgacg gcttcagcca ttaacgcgaa cctgttcgcc
     9421 gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct aatgaataaa
     9481 tccctgtggc ggcagagtac ctggggcaac atcattgtcg tggtgttgat tatgctgatg
     9541 acggcggcac tgaatttagg ctcactcgcc agcgttgcca gcgccacctt tttgatttgc
     9601 tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc ctcattgccg
     9661 attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat ctacagtctg
     9721 tggtcccagg gtagccgtgc gttgatatgg attattggct cactcttact cagccttatt
     9781 gtggcaatgg tcatgaagcg caataaaacc gtataacatc tctctgtgcg cagtacttcc
     9841 tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt gaatatctgt
     9901 tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca gcgatgtagc
     9961 aaaaattacc ggcctgacta gcaaagccat tcgcttctat gaagagaagg ggctggtgac
    10021 gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc tcaacgaact
    10081 gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg gcgagctggt
    10141 gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca cgctggagaa
    10201 ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc agctgctggc
    10261 actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg aaaatctctc
    10321 cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata tgtagggtta
    10381 tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg ctggcgctga
    10441 caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca
    10501 gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta tcgctgtgct
    10561 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
    10621 ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag accaccaggc
    10681 cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc agcatctcgg
    10741 ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg accattaact
    10801 ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agttcggcaa tcccggcaat
    10861 cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga
    10921 accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat
    10981 atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg cttcggcgga
    11041 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
    11101 ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
    11161 aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
    11221 ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt
    11281 ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat
    11341 ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat
    11401 cgccagctcc agattgctga cttcataggc agcgcgtggc gcgtcaatca cctgaataaa
    11461 acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
    11521 atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
    11581 cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg
    11641 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
    11701 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
    11761 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
    11821 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
    11881 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa
    11941 catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
    12001 caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
    12061 gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccaac aagtctctta
    12121 ctgcgcccag acaccgacgc tgtttggcga tacggtatac gataatctga tctttccctg
    12181 gcagatccgt aaccggcagc ctgacccagc catttttctc gattttctcg aacgcttcgc
    12241 cttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg aaaaacaacg
    12301 catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaatcac
    12361 cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc gttatgtgcg
    12421 cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
    12481 taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc
    12541 ataatattac taacgaatca ttggcactgg cattaatgct ggtggtggtg gcaatcttaa
    12601 ttagccataa agaaaaactg gcgctggaga aagatatttt ctggagcgtc gggcgcgcga
    12661 taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca
    12721 gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
    12781 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gttggggcgg
    12841 gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga
    12901 tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca
    12961 atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
    13021 cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
    13081 cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc
    13141 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
    13201 tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt
    13261 tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg
    13321 gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg aacaacggaa tatcaataca
    13381 acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
    13441 cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca
    13501 gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg
    13561 ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt
    13621 gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt
    13681 agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac
    13741 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca
    13801 actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt
    13861 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc
    13921 ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca
    13981 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
    14041 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt tccaggatcg
    14101 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
    14161 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
    14221 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
    14281 cttcaactca cccctgcaat attttgtcca tcacgcgccc aggcagcagg cgcttgagca
    14341 ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
    14401 aagcatggcg cactttgtcg accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc
    14461 cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac
    14521 gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc tccatgcgca
    14581 gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg
    14641 tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg tgcggtaaca
    14701 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct
    14761 gttccatctg cgcacggctg atggtggaaa gagggccata catgccgaat ccggcattgt
    14821 taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtctgct gcgcgatcaa
    14881 cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgttg ttcatacgct
    14941 caacatcatc cggttttcgg caacctgcca gcacatgaaa accctggcgt tttaattcga
    15001 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
    15061 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc
    15121 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
    15181 tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca
    15241 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
    15301 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
    15361 aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg
    15421 ccgcccagtt caaccagcac ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc
    15481 gccagtcctt gttgcgaggt gtcgccgctg atgctggcat taactaccga cgttttactc
    15541 tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg
    15601 ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa ggttaacagg
    15661 accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga
    15721 agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca
    15781 aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg
    15841 cgatcctcgc cgggcttgat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc
    15901 tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt
    15961 ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggctc
    16021 tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc gaaagcgttg ctcgaacagt
    16081 tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac
    16141 gagtggcgct ggcacgagcc tttaatggtc gacctgatgt gctgtttgcc gacgaaccca
    16201 ccggcaacct tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc
    16261 gtgaacatgg caccacgttg attatggtga cccacgacct gcaactggcg gcacgctgcg
    16321 accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg cacgttggtt
    16381 ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt
    16441 ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccagca
    16501 aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt tcacgcgaag tgccgcaagc
    16561 gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac
    16621 aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg atgatatcta
    16681 cccgatgtat ggcgatctgc aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt
    16741 attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt
    16801 gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa
    16861 ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc
    16921 cgtgcagccg gggagtcggg tcacctggcg ttataaattc ggcggcaacg agaaccagct
    16981 cgacggctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct
    17041 ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct
    17101 ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtagcga tgaatcatta
    17161 ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca
    17221 actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg
    17281 tggggccata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc
    17341 tgctgcacta ccgccagcca gcctctggcc gtggctgtgg gcgcttggca ccatgacggt
    17401 catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg
    17461 cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg aagttttatc tgccgattgt
    17521 cagtatggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc
    17581 ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt gtgctgggct ggatgcagct
    17641 gaatgtactt tgccgcatga cgctgaaatc gctacctctg cgcctggcgg ttagccgcct
    17701 gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct
    17761 gctggcgctg ttgctggtat tgcgttgcga tctgctcgac cgctggcagc agctaccgcc
    17821 agaaagcccg aactactttt taattaacat cgccacagaa caggtagcac cgctaaaagc
    17881 gttcctcgcg gaacatcata tagtcccgga atcattttat ccggtagtgc gggcgcggct
    17941 gacggcgatt aacgataagc cgacagaagg taatgaagat gaggcgctta atcgcgaact
    18001 caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg gtaactggcc
    18061 gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct
    18121 cggtggtacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    18181 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc
    18241 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    18301 gttgacgcaa ctcaaccgcc agttcccgac cattagcctg ttagatattg gcgcgatttt
    18361 aaaacaggtc ggttaggtgc tggagcaggt aagtcgggcg ctggaggtga tggtggtgct
    18421 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgccagcg
    18481 tcatcaggag ctggtggtat ggcgcacact cggtgcgggg aaaaaactgc tgcgcacgac
    18541 attgtggtgt gagttcgcca tgcttgggtt tgtttccggc ctggtggccg caattggtgc
    18601 ggaaacggca ctggcagtgt tgcaggcgaa agtgtttgat ttcccgtggg agccagactg
    18661 gcgattgtgg attgttctgc cgtgcagcgg tgcgctgctg ctgtcgcttt tcggcggctg
    18721 gctgggtgcg cgactggtta agggtaaggc gctgttcagg cagtttgcgg ggtgatgaga
    18781 gtaaataagg ggcgttctgg ttgaatggaa cgccttgttt aaattaattc tacaatatgt
    18841 ttcattgatg atgttattga attggtgatt tcctatcttt ctattgctga tattaatctg
    18901 aattggtgga gtttaataat tttaaatata gccctacaaa aataactcag actaataaaa
    18961 tcataaatca tatgcgttga atggatatta tccatatagt gaatttgttg atgatgaatt
    19021 catctgtgct aaaaatttta gtttaataaa atattgaaag tgacctgtaa taacagttat
    19081 tgttgattga gaacaaataa gtttatgtga aaaatatata aatacattag ctggtcatgt
    19141 gtgtcatttt atttttttgt tgctaacaca gggatatgaa caataactaa aagggcactt
    19201 tatatgagcg gaaaaccagc ggcgcgtcag ggagatatga ctcagtatgg cggtcccatt
    19261 gtccagggtt cggcaggtgt aagaattggc gcgccaactg gcgtggcgtg ctcggtgtgt
    19321 ccgggcggga tgacctcggg caacccggtg aacccgctgc tcggtgcaaa agtacagccc
    19381 ggcgagacgg accttgcgct gcccgacccg ctgccgttca ttctctcccg cacctacagc
    19441 agctaccgga cgaagacgcc tgcgccagtg ggaacttttt ggccccggct ggaaagcgcc
    19501 ttctgatatc cgcttacaga tacgcgatga tgcactggta ctcaacgaca acggcgggcg
    19561 gagcattcac tttgagccgc tgctgccggg gaaggctgtg tacagccgca gtgagtcaat
    19621 gtggctggtg cgtggcggta aggcagcgca gccggacggc cacacgctgg cacgactgtg
    19681 ggcgtcgctg ccgccggata tccggttaag cccgcatctt tacctggcga ccaacagcgc
    19741 acaggggccg tggtggatac tggggtgatc tgagcgggtg ccgggtgctg aggacgtact
    19801 gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggaccgct tcgggcggac
    19861 gctgacgtac cggcgtgagg ccgcaggcga cctggccggg gaaatcaccg gcgtgacgga
    19921 cggtgccggg cgggagttcc gtctggtgct gaccacacag gcgcagcagg cggaagaggc
    19981 ccggcagcag gcggtcgtcg ccggagcaaa acgaccggac attcctgatt cactgccgga
    20041 gagcacagag tacggccccg acaggggtat ccgcctttcg gcggtgtggc tgatgcacga
    20101 cccggcatac ccggagagcc tgcccggtgc gccactggcg cggtacacgt atacggaagc
    20161 cggtgaactg ctggcggtat atgaccgcag caatacgcag gtgcgcgctt tcacgtatga
    20221 cgcgcagcac ccgggccgga tggtggcgca ccgttacgcg ggaaggccgg agatgcgcta
    20281 ccgctacgac gatacggggc gggtggtgga gcaactgaac ccggcagggt taagctaccg
    20341 ctatctttat gagcaggacc gcatcaccgt caccgacagc ctgaaccggc gtgaggtgct
    20401 gcatacagaa ggcggggccg ggctgaaacg ggtggtgaaa aaagaactgg cggacggcag
    20461 cgtcacgcgc agcgggtatg acgcggcagg aaggctcacg gcgcagacgg acgcggcggg
    20521 acggcggaca gagtacggtc tgaatgtggt gtccggcgat atcacggaca tcaccacacc
    20581 ggacgggcgg gagacgaaat tttactataa cgacgggaac cagctgacgg cggtggtgta
    20641 cccggacggg ctggagagca gccgggaata tgatgaaccg ggcaggctgg tatcggagac
    20701 atcgcgcagc ggggagacag tacgctaccg ctacgatgac gcgcacagtg agttaccggc
    20761 gacgacaacg gatgcgacgg gcagcacccg gcagatgacg gcggttcacc gcgaggaagg
    20821 tatcagcctt taccgccact atgacaaccg tggccggtta acctcggtga aagacgcaca
    20881 gggccgtgaa acgcagtatg agtacaacgc cgcaggcgac ctgactgccg ttatcacccc
    20941 ggacggcaac cggagcgaga cacagtacga tgcgtgggga aaggcggtca gcaccacgca
    21001 gggcgggctg acgcgcagta tggagtacga tgctgccgga cgtgtcatca gtctgactaa
    21061 cgagaacggc agtcacagcg acttcagcta cgatgcgctg gaccggctgg tacagcaggg
    21121 cggctttgac gggcggacgc aacgttatca ttatgacctg accggaaaac tcacgcagag
    21181 tgaggatgag gggcttgtca ccctctggca ctacgatgcg tcggaccgca tcacgcaccg
    21241 gacggtgaac ggcgacccgg cagagcagtg gcagtatgac ggccacggct ggctgagaga
    21301 aatcagccac ctgagcgaag gccaccgtgt cgctgtccac tacggctacg acgataaggg
    21361 ccgcctgacg ggcgaacggc agacggtgga gaacccggag acgggggaac tgctgtggca
    21421 gcatgagacg aaacacgcct acaacgaaca ggggctggca aaccgcgtca cgccggacag
    21481 cctgccgccg gtggagtggc tgacctacgg cagcggttac ctggcgggca tgaagctggg
    21541 cgggacgccg ctggtcgagt atacgcggga caggctgcac cgtgagacgg tgcgcagctt
    21601 cggcagcatg gcaggcagta atgccgcata tgaactgacc agcacataca cccccgcagg
    21661 ccagttacag agccagcacc tgaacagcct ggtgtatgac cgtgactacg ggtggaatga
    21721 caacggcgac ctggtgcgca tcagcggtcc gcgacagacg cgggaatacg gctacagcgc
    21781 cacgggcagg ctggagagcg tgcgcaccct cgcaccggac ctggacatcc gcatcccgta
    21841 tgcgaccgac ccggcgggaa accggctgcc ggacccggag ctacacccgg acagcacgct
    21901 cacggcgtgg ccggataacc gcatcgcaga ggatgcgcac tatgtttacc actatgatga
    21961 atacggcaga ctgacggaga agacggaccg catcccggcg ggtgtgatac ggacggacga
    22021 cgagcggacc caccactacc actacgacag ccagcaccgc ctggtgttcc acacgcggat
    22081 acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctga gccgccggac
    22141 ggggaaacgg gtgtggcgtc gcgagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa
    22201 accggaggtg acgtggtacg gatgggacgg cgaccggctg acgacggtac agaccgacac
    22261 cacgcgtatc cagacggtat accagccggg gagcttcgcg ccgctcatcc gcatcgaaac
    22321 agacaacggt gaacgggaga aagcgcagtg ccgcagcctg gcggagaagt tacagcagga
    22381 agggagcgaa gacggtcacg gtgtggtgtt cccgg
//
LOCUS       DADLTD010000051        22386 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLTD010000051 DADLTD010000000
VERSION     DADLTD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.051"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            135..1241
                     /locus_tag="JN701_001547"
     CDS             135..1241
                     /locus_tag="JN701_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7364039.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1421..2215
                     /gene="potC"
                     /locus_tag="JN701_001548"
     CDS             1421..2215
                     /gene="potC"
                     /locus_tag="JN701_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY7364040.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2212..3258
                     /gene="potD"
                     /locus_tag="JN701_001549"
     CDS             2212..3258
                     /gene="potD"
                     /locus_tag="JN701_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY7364041.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3414..4235)
                     /gene="cobB"
                     /locus_tag="JN701_001550"
     CDS             complement(3414..4235)
                     /gene="cobB"
                     /locus_tag="JN701_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY7364042.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4251..5162)
                     /gene="nagK"
                     /locus_tag="JN701_001551"
     CDS             complement(4251..5162)
                     /gene="nagK"
                     /locus_tag="JN701_001551"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY7364043.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5191..6435)
                     /gene="lolE"
                     /locus_tag="JN701_001552"
     CDS             complement(5191..6435)
                     /gene="lolE"
                     /locus_tag="JN701_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY7364044.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6435..7136)
                     /gene="lolD"
                     /locus_tag="JN701_001553"
     CDS             complement(6435..7136)
                     /gene="lolD"
                     /locus_tag="JN701_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY7364045.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7129..8328)
                     /gene="lolC"
                     /locus_tag="JN701_001554"
     CDS             complement(7129..8328)
                     /gene="lolC"
                     /locus_tag="JN701_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY7364046.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8590..9663
                     /locus_tag="JN701_001555"
     CDS             8590..9663
                     /locus_tag="JN701_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7364047.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNATYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9791..13237
                     /gene="mfd"
                     /locus_tag="JN701_001556"
     CDS             9791..13237
                     /gene="mfd"
                     /locus_tag="JN701_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY7364048.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13381..14343
                     /gene="ldtC"
                     /locus_tag="JN701_001557"
     CDS             13381..14343
                     /gene="ldtC"
                     /locus_tag="JN701_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY7364049.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14424..14681)
                     /gene="bhsA"
                     /locus_tag="JN701_001558"
     CDS             complement(14424..14681)
                     /gene="bhsA"
                     /locus_tag="JN701_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY7364050.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14922..15554
                     /gene="comR"
                     /locus_tag="JN701_001559"
     CDS             14922..15554
                     /gene="comR"
                     /locus_tag="JN701_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY7364051.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15616..16155)
                     /locus_tag="JN701_001560"
     CDS             complement(15616..16155)
                     /locus_tag="JN701_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7364052.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16382..17686)
                     /locus_tag="JN701_001561"
     CDS             complement(16382..17686)
                     /locus_tag="JN701_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7364053.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18086..18628)
                     /locus_tag="JN701_001562"
     CDS             complement(18086..18628)
                     /locus_tag="JN701_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7364054.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18651..19676)
                     /gene="nagZ"
                     /locus_tag="JN701_001563"
     CDS             complement(18651..19676)
                     /gene="nagZ"
                     /locus_tag="JN701_001563"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY7364055.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19687..20511)
                     /gene="thiK"
                     /locus_tag="JN701_001564"
     CDS             complement(19687..20511)
                     /gene="thiK"
                     /locus_tag="JN701_001564"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY7364056.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20495..21133)
                     /gene="lpoB"
                     /locus_tag="JN701_001565"
     CDS             complement(20495..21133)
                     /gene="lpoB"
                     /locus_tag="JN701_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY7364057.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(21147..21524)
                     /locus_tag="JN701_001566"
     CDS             complement(21147..21524)
                     /locus_tag="JN701_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY7364058.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21527..21886)
                     /gene="hinT"
                     /locus_tag="JN701_001567"
     CDS             complement(21527..21886)
                     /gene="hinT"
                     /locus_tag="JN701_001567"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY7364059.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5570 a         5880 c         5558 g         5378 t
ORIGIN      
        1 cgttccaaga ccacaaaaaa ttcgtgggcg ttggctgata gatcgccgag cagtatttgt
       61 tgggccaggt gaaacgggaa ttgcgccgga aattcatact ggcgatgatg atgcactgaa
      121 ggagatttta actcatgtca ccgaggccac gaaaaaacag cactgacgta gccggtcttt
      181 acgaaaagtt tgatcgcaga actggcagag tttactacca gtataaaaat cctgtgactg
      241 gaaaatttca cggactcgga acagacaaag gtaaggcaga aaaaatcgct tccacagcca
      301 atcagcgaat agctgcagca gaagctgaat atttcatgcg caaaattgat gaaagtccgt
      361 cagcaacaaa acgtcggggt atcagattaa aggcatgggt tgatcgatat ctgaaaatac
      421 aggacacgcg actgaaaaat ggagatattg cagctacaac tcacaaagaa aaaactcgaa
      481 tggctgcata cctggtttcc cgtctgggaa accacccatt gaaagaactg gaagtaagag
      541 actttgcatt aatactggat gagtggctgg ataaagacat ggtcagcaca gcgagagtaa
      601 atcgtggatt atgggttgat atttataaag aagcacagca tgcaggggaa gttcctcctg
      661 gatggaatcc tccggaggct acccgtaaac cgatccctaa agtaaccaga gccaggctca
      721 ccatggaaga ctggcaaaaa atttacaatg caacgcctga aaaacacttt atccgtaacg
      781 caatgcttct tgcgattgtt actggtcagc gccgtgatga catttgccac atgcgttttt
      841 cagatgtgtg gaacgaacac ttgcatatca cccagggaaa aaccggaatg cgtctggcgt
      901 taccgcttac actacgctgt gatgccattg ggataacgtt aaaagaagtt attgatgggt
      961 gccgagacag aatattaagt ccatatctaa tccatagtcg gcaccagaaa caaccgaagc
     1021 cgatgagtaa agacaacctg agcgactact ttgccaaagc acgggatctg gctgggataa
     1081 ttccaccagc aggaaaaact ccgccaacat ttcatgaaca acgctctcta tcagaacggc
     1141 tgtaccgtgc acagggtatc gatacaaaaa cattactagg acataaagtc caggcaacca
     1201 ccgatcgcta taacgatact cgaggtcagg aatgggttta agctggttat ttgacaaaca
     1261 ccatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt
     1321 ttggggaaac gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc
     1381 tgaaagtgct ccaccttatg aataagaagg tggaactcga atgatcggtc gactgcttcg
     1441 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     1501 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     1561 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     1621 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     1681 ttatcgcttt cgcggtaagc cgttcgttag cggaatgctg tttgtggtaa tgatatcacc
     1741 agatatcgtg atggcaattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     1801 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt
     1861 gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc
     1921 cagcgaattt acaattctgc gaaaaatcat actgccactg gcaatgccag cggtggcagc
     1981 gggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtagtttctt cgttcgtcac
     2041 cggaccaagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     2101 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc
     2161 cagtcagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     2221 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     2281 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     2341 gaacagttca ccaaagaaac ctgtattaag gttatctatt cgacttacga gtcgaacgaa
     2401 accatgtacg ccaagctgaa aacgtacaaa gacggtgcgt atgacctggt ggttccttca
     2461 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     2521 ttaacgaact tcagcaatct cgatccagac atgctcaaca agccttttga cccgaataac
     2581 gactactcca ttccgtatat ctggggggcg acggcgattg gtgttaacgg tgatgcggtg
     2641 gatccgaaat ctgtcaccag ctgggccgat ctgtggaagc cagagtacaa aggcagcctg
     2701 ctgttgaccg acgatgcccg tgaagtgttc cagatggcgc tgcgtaagct gggctattcc
     2761 ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg
     2821 ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt
     2881 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt
     2941 gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca
     3001 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     3061 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     3121 ttaagtccag aagtggcgaa cgataaaacg ctctacccgg atgctgaaac cattaaaaat
     3181 ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag
     3241 ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttattta
     3301 gcaaggatgt ggctaatgag tcaggacagt agagttttat ccgactcttt cgtcggtatt
     3361 gtggtccgct cgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
     3421 cccttcagca acttttcaac aaactcaggc accacctggc ttgccgggcc gtaatatttc
     3481 tcggcaaatt cattaccgac ctgacttggt tcgagattca gttccacggt gtgcgcgcca
     3541 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
     3601 gcaatgaaaa tatcagccat cgacaacgcc atataaattt catccatgcc gagtggcatt
     3661 tcgccaaacc aaacgacgtg cgggcgcaag ggtgccggga actggcaaca atggcattta
     3721 tcttctggcg taacgtctcc ggtccagtcg agaacctgac cactttgcga acaacgaact
     3781 ttcagcaatt ccccatgcat atgaatgaca ttggtattac ctgcgcgttc atgcaggttg
     3841 tctatattct gcgttaccag caaaaagcga tcgccgagag cgtcttgcag tttagccagc
     3901 gcaagatgcg cggcgttagg ctgaattttt ggctgctgca gctgtcgacg acgggcgtta
     3961 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
     4021 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
     4081 gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     4141 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     4201 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
     4261 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
     4321 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
     4381 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     4441 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     4501 tgctcatcgc cttgattata aagcgcaata atttcgggag cctgcaactg ttgatgataa
     4561 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattctcaat gcagccatgc
     4621 tgaccacagc cgcagcggcg taacgggaaa tccaacccca tcatggttaa cgcatcaacc
     4681 ggcagacgca tatggccaaa ctcgccggta atgtagcttt taccggtaat cggtttgccg
     4741 ttgaaaatca gcccaccgcc aacgccggta ccaagaatca accccatcac cagtggatat
     4801 tgcgtaaatt catcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
     4861 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagcg
     4921 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg tataccaatg
     4981 ccgacagaac ctctacagcc aaaacgttga tcagcttcgg ctaccagctc gcacactgca
     5041 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     5101 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
     5161 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     5221 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     5281 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata
     5341 gatatcgctg gagaggaact gatgaccgat cagcttttca atccgctcaa taatcggggt
     5401 aagttgcagt gaaacaacta cgccgataat cagcccacac aggctgccga acagccctgc
     5461 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
     5521 taatactgcg atatcgccac tcttatcttt caccgccatc actaaggtgg agacgatgtt
     5581 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5641 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5701 ggtcatttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5761 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
     5821 catggcaaaa ctgtgatcga gttggccact caactgcaaa atacccgcaa cgtgcaaacg
     5881 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca tgatcgacac
     5941 ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
     6001 ttcgcccgct ttaaaattgc gccacgcatc cccctgaaca aacgagggta atgcgctcag
     6061 acgctgttcc tgttgtgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     6121 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacccg gcactttctg
     6181 cactttatcc agtgcttcct gccagttagt ccatggctga tttaccgctt cgatttcacc
     6241 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     6301 taagccgacg atcaataccg ccacaccgag ggcaatgcca atggtagaaa tgacggagat
     6361 cagcgacacc atgccgccgc gccgccgtcc gcgactaaaa cgcaggccaa ttaataacga
     6421 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgtccatca
     6481 cgcatttcta actggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     6541 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtcggcgtta
     6601 cgcgcatcga ggttaccggt aggctcatcc gccagtacca gacgcggatt attgaccagc
     6661 gcacgggcaa tcgctacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6721 gcacgatgct ccagccccac cgcttttaac atctccagtg cacggctgtt gatttcagcg
     6781 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcggt aaaatccggc
     6841 agcaggtggt gaaactgata aataaagccc agtttctggt tgcgcagttc tgcttttgct
     6901 gccgacgaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggcgta
     6961 tccagtccgc ccagcaggtg cagcaaggtg cttttaccgg aaccagagct accgacgatc
     7021 gccatcattt caccttcgcc gacgctgaaa ctgacattgt gcagtacatc ggtttgcaca
     7081 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
     7141 taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
     7201 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccatcgg
     7261 cagcgccgcg ccatcaagca ggacgccgat tattggcatc agattattta actggctggc
     7321 aagcagtgcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
     7381 caccataaag atcatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgctac
     7441 ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac
     7501 ggcgacaatc aggctcagca gtaaacccat catatttttt tccatgcgta cggcctggaa
     7561 caactcgcct ttacgatcac gccagtcctg ccatttgctg tcttcaggca gtttttgctg
     7621 acttaatgag tcgactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
     7681 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt cattgccatc
     7741 gacttcactg ttggcggcga aagtgccaat cacattgaac aggcgctgac ttggaatacg
     7801 ccccatcggc gtgaactggc tggcagatgg taccatcatg cggatttgat caccgcgatt
     7861 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
     7921 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccggatcgat
     7981 acccagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
     8041 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
     8101 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
     8161 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
     8221 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
     8281 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
     8341 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
     8401 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
     8461 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
     8521 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
     8581 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
     8641 ccggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac ttttccgcat
     8701 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcacccttt
     8761 cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta
     8821 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
     8881 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
     8941 ttaagcaatg cctccaatgc cacttatgcc gattccaccg gtgagttcct gcacgggatg
     9001 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
     9061 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
     9121 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgccttat
     9181 tacagccttg gcgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtaccgttg
     9241 cgccgccatc tgctgatggc ttctttgctt accgttctgg cggtcggtgc ctggttgttt
     9301 actatctcgc tgctgttgtc gctggtatcg attgtggtga tcatgaagct gttttatcag
     9361 tacgagcaac gattcggtat gcgctccacc agcctgctga atgtgattgg ctccaacacc
     9421 attgctatct acaccaccca tcgcattctg gttgaaatat tcagcttaac tctgcttgcg
     9481 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttatccc
     9541 tttgtcagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcgt
     9601 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ctgccgtcag ttactcccgc
     9661 taagccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
     9721 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
     9781 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
     9841 tctgctgggc gagttaaccg gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg
     9901 tcacgccggt ccggtggtac tcattgcacc agatatgcaa aatgctctgc gtttgcatga
     9961 tgaaatcagt cagttcaccg atcagatggt gatgaatctg gcggactggg aaactcttcc
    10021 ctacgacagc ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    10081 gctaccgacg atgcagcgtg gcgtactgat tgttccggtg aatacgctta tgcagcgcgt
    10141 ttgcccacac agttttctcc acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc
    10201 acgagatgca ttacgaaccc aactggacag cgccggttat cgccatgttg accaggtgat
    10261 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    10321 gctgccttat cgtcttgatt tctttgatga tgaaatcgac agcctgcggg tgtttgacgt
    10381 cgacagccag cgcacgctgg aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt
    10441 tccgaccgat aaagcggcaa ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    10501 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    10561 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    10621 tgccaatacc ttgctcgtga atactggcga tctggaaacc agtgccgaac gtttccaggc
    10681 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc
    10741 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgggt
    10801 gcagctaaaa actaaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    10861 actgccagac ctagccgttc aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    10921 cctcgagact ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc
    10981 gctgggtgaa ctgctcgcac gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    11041 agccagcgac cgtgggcgtt atctgatgat tggcgctgcc gaacatggtt ttgtcgataa
    11101 ggtgcgtaat ctggcgctga tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg
    11161 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc ttgcggagct
    11221 gcatattggt cagccggtgg tccatctgga gcacggtgtc gggcgctatg ccggaatgac
    11281 cacgctcgaa gcgggcggca ttactggcga gtatttgatg ctcacctatg ccaacgacgc
    11341 caaactgtat gttccggtgt cgtcactgca tctgattagc cgttacgcag gtggcgcgga
    11401 agaaaacgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    11461 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    11521 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    11581 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    11641 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    11701 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgttggtgcc
    11761 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    11821 ggtacgtatc gaaatgatct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    11881 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    11941 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    12001 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    12061 gccgatccca cgcacgctga atatggcaat gagcggaatg cgtgacctgt cgattatcgc
    12121 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    12181 ggtccgggag gcgatcctgc gtgaaatttt gcgcgggggg caggtttatt atctctacaa
    12241 tgatgtggaa aacatccaga aagctgccga acggctggca gaactggtgc cagaagcgcg
    12301 gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    12361 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    12421 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcagctgca
    12481 ccagttacgc ggtcgcgtcg gacgttcaca tcatcaggca tatgcatggc tgctgacgcc
    12541 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    12601 agatctcggt gcaggctttg cgctggcaac gcacgatctg gagatccgcg gcgcgggtga
    12661 actgcttggc gaagaacaaa gtggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    12721 gttgctggaa aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct
    12781 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    12841 tcctgacgtg aatacgcgtt tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    12901 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    12961 gcgtaccctg ctggatgttg cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa
    13021 gctggaaggt aatgagaaag gcggggtgat cgaatttgcc gagaagaatc acgttaatcc
    13081 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    13141 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    13201 gtttatgcgt gaactggaag agaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    13261 gcccccatcg gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    13321 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    13381 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    13441 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    13501 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    13561 aaaaaatata acgtcggctt tctcgctctg ttacaggcta accccggcgt tgatccttac
    13621 gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg
    13681 cgcgaaggca ttgtgatcaa cattgcggag ctgcgtctct attactaccc gccgggtaaa
    13741 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    13801 acaatggtga ccaccgtttc agacaaacgt gcaaacccaa cctggacgcc aacggcaaac
    13861 acccgcgccc gttataaagc acagggaatt gagttgcctg cggtagtgcc ggctggaccg
    13921 gataacccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    13981 catggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    14041 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    14101 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    14161 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    14221 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    14281 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaactctg
    14341 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    14401 agcaggctaa tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc
    14461 ggaccggtta cagaagtaat acggaaagat tttgcaccca tctcatccgc tttttgcgcc
    14521 agctgctctt ccagcgatcc cagatttgtc cccgctttag cactgattgt accgactttt
    14581 tgttggcctt ctggcgttga ctgaacttcg acagccgcaa agctggcaaa tgacatggag
    14641 cttaaaatcg ccgcagcgat gagggttttt acgtttttca taatagtggc cttatgcaga
    14701 tgaatgacgt cgcaagcaat tacttaacga tcgataacta aatgacagat gtgatctgga
    14761 tcacatacaa gatatttttt ataacaatca ttaattaatt taaaaatctt taaatttcag
    14821 ttaaatagaa agcaattatt tttatgggat ctggactggt gaacggaggt gattattttt
    14881 ataataatct ttcattcaca gtcaccaggt acaacggcat catggcaact gactcaacac
    14941 aatgtgtaaa aaaaagccgt ggccgcccaa aagtgttcga cagggatgcc gcgcttgata
    15001 aggccatgaa attgttctgg caacacggtt atgaagcgac ttctcttgcg gacctcgtcg
    15061 aagcgaccgg agccaaagcg cccacgctat acgcggaatt taccaacaaa gaggggttat
    15121 ttcgtgccgt tctcgaccgc tatatcgatc gttttgccgc taagcatgaa gcacagctgt
    15181 tttgtgaaga gaaaagcgtg gagtctgcgc tggctgacta ttttgctgcc atcgccaact
    15241 gctttaccag caaagacacc ccggcaggct gcttcatgat caacaactgc accaccctct
    15301 ccccagattc aggagatatc gccaatacgt tgaaatcacg ccatgcgatg caagagcgca
    15361 ctttgcagca gtttttatgt caacgacaag cgcgcgggga aatcccgacc cactgtgacg
    15421 tgacacatct ggcagaattc cttaattgta ttattcaggg gatgtcgatc agcgcacgcg
    15481 aaggtgcatc gctggaaaaa ctaatgcaga ttgccggaac gactttgcgt ttatggcccg
    15541 aactggtgaa ataagtcgca aaaaaagccc ctcagcctga gcgaatgagg ggccaaattg
    15601 cagagtacag ccttgttata ctttgttatt caaaatcagt tgcccattac tatctagcgg
    15661 gatctgcgta cccggatcgc ggtccatgcg gattttgccc tgctgatcgc caatcttata
    15721 ggtcacatca taaccgagca ttttttctga cttgtcatac accgttttac aacgctgttg
    15781 cgtagtcgtg taagtatcgc tttcctggag agagccctgg atctggttac cggcatatcc
    15841 accacccagc gcccccacaa cagtggcgac atctttaccg cgaccaccgc caaactgatg
    15901 ccctatcacg ccgccagcaa cagcgccgag caccgacccg gtaatgcgat tttcatcctg
    15961 caccggtcga cgatgggtca ctgtgacgtt gcgacactcc tgacgcggtg ttttaaccgt
    16021 ttccttgatt ggggttgcag aaacaacctg agcgtattgc gggccacgtt caaacacgtt
    16081 cagactggcc actgccgcta cgcccagcgc agctgcgaca ccaatcccga tacccgccaa
    16141 cattgattta ttcacgggaa cacctccttc tttgcagtta tgccaatcat gcaagcgaag
    16201 gagaaaagca gctatcagac agaagatgaa aagcgcggga atggctggaa atacttaatg
    16261 attcggagga gtctgaagga atgcctgatg cgacgcttaa cgcgtcttat caggcctacc
    16321 gctcgtacca tttgtccagg gcggatgagg cgtttatgcc acatccgcca gtgtacgtcg
    16381 attaatgcaa cttcaaacgc ggacggataa cgcggttaat actccccacc agcatcatta
    16441 atccggtttt aaagtaacca tgcagcgcaa tctgatgcat tcggtatagc gatatatata
    16501 caaagcgcgc aattcgtcct tcaatcatca ttgagccgcg cgtcaagtta cccatcaggc
    16561 taccaacggt ggagaagttc gacagcgata ccagtgaacc atgatcttta tactgataat
    16621 ttttcagcgg cttaccgttt atctgcgcca gaatattgtt cattgcgcaa gtcgccatct
    16681 ggtgtgcagc ctgagcacgc ggcggaacaa agcccccttc cggacgcgtg catgacgcgc
    16741 agtcgccaat agcgtaaatg tctggatcgt gggtggtttg cagcgtcggt tccaccacca
    16801 gctggttgat acggttagtt tcaagaccac cgatatcttt caggaagtct ggcgctttga
    16861 tcccggctgc ccacaccatc agatcagcct caatatattc accatcttta gtgtgcaggc
    16921 cgccttcatc agcactggtg accatggttt gcgtcagcac gcgaacgcca agtttcgtta
    16981 actcactatg ggccgcagca gagatgcgtg gcggtaatgc aggcaaaata cgttctcccg
    17041 cttctactag cgttacgttc agggcttcgt tggtcagacc tttataaccg tagctgtgca
    17101 gttgcttgac cgcgttatgc aactcagcgg agagttctac acccgtcgcg ccgccaccga
    17161 caatcgcaat gttcactttg ccattcgcgc ccaggttggc ggagtatttc aggaacagat
    17221 tcagcatctc ctggtggaag cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct
    17281 ctttgacgcc tggcgtattg aaatcgttag aggtgctacc cagcgccatg accagggtgt
    17341 cataggcgat tttacgttcc ggaaccagca gttcaccttt ctcgtcgcgt agttctgcaa
    17401 tagtgattgt tttcgcttca cgatcaatat caatgacgga acccaactgg aactggaaac
    17461 catgattgcg cgcatgggcc agatagctca acgcatcgac gccttcatca agcgagccag
    17521 tcgccacttc gtgcagcagc ggtttccaca ggtggctgtg gttacgatcg accagcgtaa
    17581 ttttggcttt tttcttacgt cccagcttat gccccagctg tgttgccatt tccagcccac
    17641 cagcaccgcc gccgacaatc acaatctttt tcaatggcgt agtcaacgtg acccccttaa
    17701 atttattaac caattgttaa ttaatggtta ttaacatagc tcttaattaa caacaggtta
    17761 cagcaatgaa aatgttcttc gctattgaga ataacatgaa tggtgcattg gtcataccaa
    17821 aattgatgtt aatcaagttt tgttgctgaa aagataggcg actttaccgg gtgagatcaa
    17881 aaaatagagt ttattgttta ttatttgagc gaatttgtga gagtgatata gagtgagggg
    17941 tgtatgcagg gcgttcggta ggcctgataa gaggcgcatc aggcattgat gctcgactgt
    18001 cggatgcggc gtaaacgcct tatccgactt acagaattcg gagcgcatca ggtaccggca
    18061 aggttacctg atgcgacgag ggcatttacc cgagggtttt gaacgcttta atgcgctgca
    18121 aatgcgggga gatattcttg aatttgtgcg tctgctcttc gtcccagaca atctcgtaat
    18181 aatgatgcaa ctcttcagat gtccgctggc tgttaagcgc ttcatcatta cgcgacaaaa
    18241 tcaccaggca acgatcgcga ttcttctcgc ggaagttagt cacacactta gtggcaatat
    18301 cggcatactc ttccgggcga tcaatcttgc cttccatgtt ctcataaggg aacaaattag
    18361 ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat ccgttccgcc cagtatccgc
    18421 ccagaccaac gccgcaaatc agcggacgct cgtcgacgtt cagttgcaac attttgtcca
    18481 cttctttaag cagatgctgc atatcatgtt tcggatgccg cgtactgtag cttatcaagc
    18541 gtacatccgg gtcaataaac tgcaattgta agactttctc gtggttaccc ggactgttag
    18601 agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca taagccaggg ttagtgacct
    18661 gctttctctt cctgccagcg ttcatgtaac tgattcagac gggtgctgct cgccttccag
    18721 cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt tatgatacaa acgtgtaaca
    18781 cgttctgcct tgatcggtga cagattatct aacacgctga cggccccttt acgattattg
    18841 cagaccagga tcatatcgca acctgcgtcc agtgatgcct gcccgcgttc ggcataactg
    18901 cccataatcg cggcaccttc catcgataaa tcgtcagaga aaattacacc gtcaaaacac
    18961 agttcctgac gcaaaacggt tttcagccag taagaagaac cgctcgccgg acgcggatca
    19021 acatcactgt agatcacatg cgcaggcata atggcgtcga gtttattttc gcggattaac
    19081 gtgctgaaaa ccgacatatc tttggcacga atttccgctt gcgggcgtgg gtcgcacggc
    19141 gtctctttgt gtgaatctgc agttactgca ccgtgtcctg ggaagtgttt cccggtcgtt
    19201 ttcattccgg cttcatgcat cccatcaata aaccgactag cgattgccag ggctttttct
    19261 gggtcggcat gataagaacg ctcgccaatc gccgcgctga tatgtcctac atccagcact
    19321 ggcgcaaagc tgatatcaat atccatagcg atcatttcgc tggccatcag ccaacccgcc
    19381 tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg cagcaaagga ttgtgctgcc
    19441 ggtaagcggg taaaaccttc gcgaaaacgc tgcacgcgtc caccttcctg atctaccgcc
    19501 accaccagat gattgcgcga tgctgcgcgg atctggcgca ccagttcacg taactgggca
    19561 ggatcatgat agttacgcgt aaagagaatc agccctccca ccagcggatg cgccagtatt
    19621 tcacgctctt ccgcgtccag ttcataacct tcgacatcca acattactgg acccacactg
    19681 ctctccttat tgttttatta atagctgccg ccaggtgtca tcggccagcc tgataaattg
    19741 ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct gctttgagca tcagcagcca
    19801 gggaaaccat cgcctgacct gacgccataa ttgcgccgga taaatcttcg cgcgagtggc
    19861 atagtcattg accaattgcc ggtgctgttc agtattttcc acccacaccg ccgccagttc
    19921 cagcgcggta tcaccatctc cggcatactc ccagtcgatg agttttaacc ctgatgcgct
    19981 atgcactaaa tttccggcgt ggacatccat atgcaatgga cttaagcgta aaggtcgtgg
    20041 ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa cccacagtcc gccgcgccgg
    20101 atcgctttgc tgccagtaca gttccagtaa cggcaacagc gttattcgcc agccaaaacg
    20161 tggttgttga tgtagataat acagcaagcc tgccagttcg ttggtatccg gcaaatacgt
    20221 ttttaccgcg ccgggcagat agtcgactac catccagtca cggagatata aatgcggctt
    20281 cggtgcaatg cttgcgggta gttgtgataa agcccgatac tggcgcaaga acgcggactg
    20341 cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc tggccctgat gttcaatgag
    20401 aaaactaccg ccactaagcc cactattaaa cgtcgtgacg ggatgaaact gcgggaaaaa
    20461 gcgcgacagc aattcgtcgc gcgtgatggg attattactg cgaaacggca cctttacctg
    20521 accagataat ttcgcccgtc tgcaccagca tcagctgcat ttgtagggtc ggagcgttaa
    20581 cgttgccaga ggcgctggag tacagcacgt aatgagcgcc gacattgcgg gcaatgccta
    20641 tggctttact acgggtgcct aaactgtcct gcggcgacaa acctaactgt tgcttagcca
    20701 tcgacagttg ctgggcggaa accagggtaa atttcccgtt attagccagc gcatttcgca
    20761 gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt acggttgtta acgctatcaa
    20821 ccagcaggac gctacccgca gtcaccccgt cagccccaag catcttactg actatcggct
    20881 gcattgcgcc attccagtca taatggcgaa tatgcggcgc aggcggtgcc gttcgatctt
    20941 cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac cgagggcact gtggggacag
    21001 gctgttgtgg ctcggctggt tgttccggcg ctggtttcac ttcttctacc ggtgcaggtt
    21061 cacgttgccc cacacacccg gcgagaaaca tcgccagcgc ggtaatcaag gcgtagcgac
    21121 tcatttttgt catcaagatt caccccttac aaatatagat aaagtctgac tttgtgcgcc
    21181 cccagaaaat tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg
    21241 cgtggccttt ccagaggatg catctccagc cctctggcgt cataccagta aaaacgataa
    21301 tgaacggtaa cgggtttttg cctttcgtta taaagcgttg aggatgctga aggttgaata
    21361 tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa agatgactcc
    21421 atcaccagcg attgctcatc attcaccgga atttccggat gtgaacgaca gcccaccagc
    21481 agtaacaaca ccagagacac cagcccaaag catccttttc tcatcgttac agacctttat
    21541 gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg tggtaaacct
    21601 cttgtccgcc atggcggttg gtattcatga tcagacgata gccatcttcg gcaatacctt
    21661 cttgctcagc aatttttgcc actacggtga tcatgcgtcc cagcgcctgc tcatgctcag
    21721 ctgagacgtc gttcacagtc ggaatgagga tattcggaat gatcagaata tgcgttggcg
    21781 cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg atatcggagg
    21841 ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca ttttcctttt
    21901 tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat agagtatggt
    21961 cgagttaaac ctgctctttc aaccttaact cacgatttct taagcaaaaa atcgttttgc
    22021 tgccgtatat atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc
    22081 tgtttggttt acacgacaaa tatgaatgcg tatatttctc atttgcattt acaaacaaaa
    22141 ttattcgcac actaaatata atcgctcatc accctcctcc ggcatgagcc tgacgacaac
    22201 ataaaccatg agatttcaaa tgctttcaac acaatttaac agggataatc aatatcaagc
    22261 catcaccaaa ccgtcactac ttgccggttg catagcactg gcactattac cttctgccgc
    22321 ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca cagctccaga
    22381 tgatgg
//
LOCUS       DADLTD010000052        22279 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLTD010000052 DADLTD010000000
VERSION     DADLTD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.052"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(19..94)
                     /locus_tag="JN701_001568"
     tRNA            complement(19..94)
                     /locus_tag="JN701_001568"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:His,seq:gtg)
     gene            complement(153..229)
                     /locus_tag="JN701_001569"
     tRNA            complement(153..229)
                     /locus_tag="JN701_001569"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Arg,seq:ccg)
     gene            complement(332..1717)
                     /locus_tag="JN701_001570"
     CDS             complement(332..1717)
                     /locus_tag="JN701_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7364060.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JN701_001571"
     CDS             complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JN701_001571"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY7364061.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JN701_001572"
     CDS             complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JN701_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY7364062.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JN701_001573"
                     /gene_synonym="wecF"
     CDS             complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JN701_001573"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY7364063.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JN701_001574"
     CDS             complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JN701_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY7364064.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="JN701_001575"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="JN701_001575"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAY7364065.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7463..8137)
                     /locus_tag="JN701_001576"
     CDS             complement(7463..8137)
                     /locus_tag="JN701_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-fucosamine acetyltransferase"
                     /protein_id="HAY7364066.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JN701_001577"
     CDS             complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JN701_001577"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7364067.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JN701_001578"
     CDS             complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JN701_001578"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7364068.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JN701_001579"
     CDS             complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JN701_001579"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY7364069.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JN701_001580"
     CDS             complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JN701_001580"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY7364070.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTDDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JN701_001581"
     CDS             complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JN701_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY7364071.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JN701_001582"
     CDS             complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JN701_001582"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY7364072.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTCLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JN701_001583"
     CDS             complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JN701_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY7364073.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JN701_001584"
     CDS             complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JN701_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY7364074.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JN701_001585"
     CDS             complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JN701_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY7364075.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16974..18239
                     /gene="rhlB"
                     /locus_tag="JN701_001586"
     CDS             16974..18239
                     /gene="rhlB"
                     /locus_tag="JN701_001586"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY7364076.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEVLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18373..19857
                     /gene="gppA"
                     /locus_tag="JN701_001587"
     CDS             18373..19857
                     /gene="gppA"
                     /locus_tag="JN701_001587"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY7364077.1"
                     /translation="MGSISSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JN701_001588"
     CDS             complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JN701_001588"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY7364078.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         5594 a         6107 c         5542 g         5036 t
ORIGIN      
        1 tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca
       61 caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt
      121 actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga
      181 gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt
      241 gcgggggtgg ataatacgga cttcccaccc ctctgtctag tcccttttta aataaattgc
      301 gcgtttggtt acggtttgtg cgttttgctg cttattcctc cagtttatgc gctttcccgt
      361 ggcgattaag gccaaaaact ttataaatcg ccgtcaccgc cagcataaag atgatgccaa
      421 caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg caaatcagga
      481 atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga tggctggcaa
      541 tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca aaccatggca
      601 ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc ggattgggaa
      661 tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc acacccgcaa
      721 ctggtacgcc gtgacgggaa actttcgcca tcgccgccgg taactgacgg tttttcgcca
      781 gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag agcgcagccg
      841 tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg gcaaaagtca
      901 gtacgaacgg gctgccgttg ctgccacttt cattccacgg gaagatggtg acgataacga
      961 aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac tttgcctacg gcactgcgca
     1021 acgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc acgccctggt
     1081 aggacgccac cacaatacac agcgcggtca ggaacccttt ccagccaccc gcaaagaaac
     1141 cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca aagccaaaga
     1201 aaatcacgcc caggccaatg acaatcatca cgataatcgt ggtgactttg atcatcgcga
     1261 accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac gccaccagcg
     1321 ccactgcgat caatgcgggt atccactgcg ccatctccgg gaaccagaac tggacataaa
     1381 cgccaacggc ggtgatttct gagatcccca ccgccatcca cataaaccaa taagaccagg
     1441 cggtgagata accaaagaac gggctcatat aacgatgcgc ataaacggcg aacgaaccgg
     1501 taaccggttc gaggaacaac atttcgccca ttgaacgcat gatgaaaaag acgaatagcc
     1561 cggcgatgat ataggccaac aatacggatg gcccggccca tttcaaggta ctggcggccc
     1621 ccataaacag gccgacgcca atggtgcccc ccagggcgat gagttcgata tgtcgagctt
     1681 ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat
     1741 gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa
     1801 ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca
     1861 gacaggcgac ggagtgacca ctccgtcgct ttacaaagag aggaaaatca gaggttgccg
     1921 gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc
     1981 gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt
     2041 acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc atctggatgc
     2101 accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc aacggtgacg
     2161 atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc gggtttaaaa
     2221 taaccatcct gactgccaac gatattcaca ttccactggt tgcgcagttt agcttcagtt
     2281 tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc tttgcctgcg
     2341 cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg cgcctgcggg
     2401 tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa ttcggcagcg
     2461 ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc agcattaatg
     2521 gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag ggcgtgctgc
     2581 atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg
     2641 ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga
     2701 gtccggcgct ttcaaaaagc cagtacaaca gttttgcgat catcagacat gcgccgaaga
     2761 ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgt gccagcacga
     2821 tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta tagcgattag
     2881 gctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcaga ccaaccacga
     2941 tcgccccgag cgggatgaac aacgcgccgc ccatcaccac cagtgagcct ataagcgtag
     3001 gcgagatcgc cagtccggag tggttattca gcacttccct ggtaaagtag ttggctgagt
     3061 tcagcaccat actcgggcga cccggccaca gccaggaagg gataaagaca tagaaatcgc
     3121 ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac agcaacgcca
     3181 gattctccca cggcgagaag gtgtcgcgag tgagatagag aaacgtatag aacgcttcat
     3241 cgccgctcac attcattcca tagcgtttta gcgccagcca gaacatgccc acaatcccca
     3301 gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg ccaataaaca
     3361 ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc atataagtca
     3421 gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgcta tcctggcgca
     3481 gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc
     3541 cggagacttc actggagaag atctgactgt aggagttaag ccggaacagc aaaaagccgt
     3601 tgtgcatgaa gaagatgccg acgcttacca gcgcgatgcc catcaggatc acccacgtaa
     3661 gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca gcaacgcgtt
     3721 tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc gcagaaagca
     3781 acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat accagcacgc
     3841 tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag aagacattga
     3901 agttaaagcg cacacggcga aactcaaacc aagtcagcgt ggcaataaac agcgtgcaga
     3961 gcagccagac cacaaacagg ccactgaatt gcagcagact catgcgacct ccccggcggc
     4021 aatcgccaac acccgctgcc agccttgtag atagttaggg ctaaagaagg caatggtgtt
     4081 tttatccacc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc
     4141 ggtagtaaac agcaccggca aatgctgttc cgtcatatcc tgccagaacg gattttcccg
     4201 gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg
     4261 gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat aggcgtcaaa
     4321 ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca gccccgcctg
     4381 acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca ccaccacttt
     4441 taccgtatcg ccaaattgct gatgaacggc gcgcaaggca gcaatatggt cattgctgcg
     4501 gtcaccggag ttccccacca gaatggtcat tttcccttca cgttgccgat cgttcgccat
     4561 cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc agttcgcccc gcacctttgg
     4621 atgcgttttg gcaaaaaagc tcaaatcacc gcgggtggca aatacacagc cgactcgctt
     4681 ttgcgccagg cgacgtagcg ggtaaaaaag cttatatctc aagccactgg aaagctcata
     4741 caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac cactcagcag
     4801 agccagccac agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct gacggttagc
     4861 ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa attgcacaga
     4921 aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa actcgcgcgc
     4981 gtgctcgctc gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg
     5041 agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa
     5101 aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc ctgcgccgcg
     5161 cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa agtgaactgg
     5221 ctgacttccg ccagaatata aaaccgcagt gacgctttgg cgatcaccag ataaccaaag
     5281 acataagcgc ccacttttag cacatcaccc actaactgcc aggcaaagag atcgcgcata
     5341 gcggtaaatt tattcgacaa cagcaaccag atagcaaaat cacgcagcaa ccagacggta
     5401 aaactcgccg ccgccactgc cggtaagacg aattttagcg atttaaccac ttcccgggtg
     5461 atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct gaacgatgcc
     5521 gtaatgaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat ccccacttca
     5581 tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac aggcaaggtc
     5641 accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag accgttatcc
     5701 cagctgggtt tcagatagct taacgggatg acaccacgtt tgatcaacat gatggcggca
     5761 ggaattacca ccagcgcggg aatcagcgcc agacccagca acgccccttc ataaccgccc
     5821 aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat cagcgataac
     5881 gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt gccccaggcg
     5941 atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt attaccaaac
     6001 aatccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag cgtagagaaa
     6061 ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg attatcatgg
     6121 tactgggcaa cgtatttggt tacaccgtta aagatgccag ccccggcaag cacgccgagc
     6181 acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggacc aaatgacacc
     6241 gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact ggccgccgtc
     6301 cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag tcgcaattac
     6361 cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca ggcgctcgct
     6421 ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagtgttccc ccgcagggca
     6481 accgtgcagc ggaatgtaat gaaacaccgc catgatttcc gcttctttca gaaagttaat
     6541 caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg cgttctgcac
     6601 gcagccatca ggaatcgacg gcagctcgat acgcccggct ttcgccagag gcgctaacgc
     6661 atcgtagtaa ttttgccaca gcgccagacg ttgctggttg atacgatccg ctgcttccag
     6721 ttgcgcccac aggtatgcag cttgcagatc ggacatcaaa tagctggagc caatatcgcg
     6781 ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc ccttttcacg
     6841 gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc
     6901 acccgccgtg tagtttttgg tttcatggaa gctaaagcag ccaatatgac caatggttcc
     6961 cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcca ccacaaacaa
     7021 attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac ccgcgtaatg
     7081 gaccggcacg ataacgcgcg ttttgtcggt gatcgctgct tcaatcaacg tttcgtcgat
     7141 gttcatggtg tccgggcgaa catccacaaa gacgattttc gcgccacgca gcacaaaggc
     7201 attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag gctggatatc
     7261 gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta acactttggc
     7321 gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc catcgccaca
     7381 cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca ccaccggcgg
     7441 tgcgttaaat ggaatcatgt gattacctgt ataaccagta cgcggtgctt tctacattcg
     7501 caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc gccacccgca
     7561 aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg
     7621 caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc cgtaaagaga
     7681 catagccgcg aatatcgccg gacgccgcac gtagaatcag acattgatga tcaaaggtgc
     7741 cgcgcacggc attttcaatc cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc
     7801 acggcgcacg aaaacggctt tgcgcaaatg cggcgctagc taactgacgt aatgcgggaa
     7861 tatcggtctc ttgtgccacg acagcaccgc tatcactgac attgttcacg ggtagcgcca
     7921 aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc agttcacccg
     7981 tatttgatgc cgcaattttg gcctgcaccc gtgaccacgg cgctaacgcg tctggcgtca
     8041 ggagcggtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt
     8101 cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag cagatattgg
     8161 ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc atcgagccag
     8221 ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg tttttctact
     8281 gtctgcacaa aggtactggc ttcaatcagg ctgtcgtgag tgccagtgtc cagccaggca
     8341 aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat ctggttgatg
     8401 gaggtaatct ccagttcacc acgctccgac ggcttcacct gctttgcgta ctccacgact
     8461 ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg ttttggcttt
     8521 tcttccagcg agatagcgcg gaaattgtcg tcaaactcca ccacgccaaa gcgttcctgg
     8581 tccatcacct gatagccaaa taccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc
     8641 ttcggactga agccctgacc gaagaagatg ttatcgccca gcaccagaca agaaggttca
     8701 ccgttgagga aggtttcacc gatgataaag gcctgcacca gaccgtccgg gctgggctgt
     8761 tcggcgtatt ccagctggat accgaactca ctaccatcgc ccagcaggcg ctggaaataa
     8821 cctttatctt ccggcgtagt gatgatgaga atttcgcgga taccggccaa catcagcacc
     8881 gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt cgatacgccg
     8941 cgcgtaatcg gatgcaatcg ggtgccggaa ccgcccgcca ggataatacc tttcatgttt
     9001 gcctccgctg aaaattagcc tttcagacct aaacgctcgc cctgatagct gccgtcctgc
     9061 acctgcttcc accagctttc attagccaga taccactgca ccgttttacg cattccactt
     9121 tcaaaggttt cctgtggcag ccagccaagt tcacgggcaa ttttcgaagc atcaatggca
     9181 taacgcagat catgccccgg acggtcagcg acaaaggtga tcaagtcacg atagtgcacc
     9241 acgccgtgcg gcttgttcgg agccagttct tccagcagct cgcaaatggt ttccacaaca
     9301 tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac tttcccggtg
     9361 gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc acggatttgc
     9421 tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat catcagcggg
     9481 atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt gataagcgtc
     9541 ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc ttttgacgcg
     9601 gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc
     9661 aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga ttttttatct
     9721 tccgtcagcg tattccagta agcccgcgcc gcttcaagca atgtataagt cccgacaatg
     9781 ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct ttcggctgcc
     9841 agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg
     9901 cagatatcaa ctttctcaaa ggcaaagcga tcgctttgcg cgaccggtgc cagcgacatc
     9961 aggtttccgg cgtaggtcag cttatcgacc actaccaccg cgtcgctcgt ttcgttgatg
    10021 atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag aatttttctc
    10081 atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg tcgccattga
    10141 taactttaaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt gccagcgcct
    10201 cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg tggatgttag
    10261 gctcaacaac cagagtttcg ccgctatgcc actgggcgat cagttcagcg atttccatcg
    10321 ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag caggcgattt
    10381 tcagttcact ggcgcgttta tcggtagccg ccaggcaatc agccaccgcc gctttcacct
    10441 gatcgataac ccagaacggt ttgtgatcgt tcacttcgcg cgcggtacgg ataagccgcg
    10501 cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag tgaccgccca
    10561 cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg cgaatcagtt
    10621 cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca aaagcgatat
    10681 tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc gagttagtga
    10741 cgacacactc accttcgagg aaaattttgt acagttcgct ggcgcgggtc gaacaaaccg
    10801 gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc tggcccggta
    10861 acacgcgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga
    10921 aagtgagatc cggacgcatc tctgctaacc attctgccat cttctcggtt gaccccaccg
    10981 gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca atggagcgag
    11041 cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc gtgggtacag
    11101 caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta
    11161 cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg ccacgattga
    11221 tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa
    11281 acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac
    11341 tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg cctgaccatc
    11401 accatacggg ttatgggcgc ggctcatagt ttgatattcg ttttcgtctt ttaaaagacg
    11461 cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg tacccgccgt
    11521 caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc ccagcgaagg
    11581 cgcttcttcc tgaatgccgc ctgagtcggt caaaatcagc caggcgtggt tcatcaacca
    11641 gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat gccccagaat
    11701 gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa tctggatgtc
    11761 ctggtgcgtg gtggcaatgt ctgccaacgc gtggcagatt tcttcaaaac cacgaccgaa
    11821 actctcacgc ctgtgaccgg tcaccagaat catcttttta ttggggtcga taaacgggta
    11881 atttgccacc agttctgaac gcagcgtgtc gctgctcatc acctgatcac gcacccataa
    11941 cagtgcatca atgactgtat taccagtaat gaagattcgg ctatccgcaa cgttttcacg
    12001 cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca gatgcccggt
    12061 caatgtacgg ttagcctctt ccggccacgg cgaatagaga tcgtccgtgc gtaaaccagc
    12121 ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg ccagcgtcgt
    12181 tgttgtgtcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa taggttttag
    12241 cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca ttatgttgag
    12301 atcgtagtca ggtacaatgg aaaagagttt cagcacctga tcgagcatct cccgatgctg
    12361 cgcagtgacg caaactttag cctcaaaaaa aggagctttt gccaacgcat gcaccaacgg
    12421 agccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca catcgattct
    12481 cttcgaataa gcggcgagcg cctttgcgct cgccgcagca gtgttgctat ttcgagcaac
    12541 ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac ataatcatca
    12601 ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat
    12661 aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac atggcccgat
    12721 tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc agtcgagcct
    12781 ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg gcaggcacat
    12841 ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt
    12901 tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc tgagctttca
    12961 tctggatggt gcgtgccgcc catgcgcctt tcagctcatc attcagatgg ctggctgcac
    13021 gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc gcggtttcgg
    13081 caataagctt cacgctgtca ttgaccgcgc gggtaaagtc tccggggata aactggatgt
    13141 tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc atccgctgtt
    13201 tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc agttgcataa
    13261 caaactcttt ataggcttcg tccatgaccg atggttgatc ggcagaagcc atgtttgaac
    13321 ggacatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc atattcaccg
    13381 ttggacgatc ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca
    13441 gcgcgattaa cgcaaacgcc agccccatgc caataatcca tagcttccca gcccacaagg
    13501 tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt ttcccaggca
    13561 ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac cacgatttct
    13621 acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac gcttaatgca
    13681 atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact ccgggacaaa
    13741 atgagaatat tctgccagca cgccaatgga agcgagcagt gctgcggcaa gggtaatcag
    13801 cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa tatgctgacg
    13861 gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg ccaccatatc
    13921 cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat gggttttgcc
    13981 ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac tgcccgcatc
    14041 acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga tgtatggcag
    14101 gatggcggcg atcatcgcaa agcaccagat tgcgaggcag gtttgcccgt cgaaccacaa
    14161 aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc catcaatgcc
    14221 atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca ggaagtaacc
    14281 aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac tgagataaag
    14341 tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac ggatttttac
    14401 gctgatatca aaacggtcat ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag
    14461 atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc aaatccctgc
    14521 gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc ggaagtttgg
    14581 tttatccact aaaccgactt tttttgccac cttacgagca aaaaacagaa acagtgtcgt
    14641 gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa tgcatgctct
    14701 cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag ggaaagtccg
    14761 ttccgaaccc accagtcctg tgcaaaaatt aaccatttga tatgcaaatt gcataacatg
    14821 ccagcaaatt ccaggaattt tcctaacatc gccagcgcgg cattaagatt acagatataa
    14881 aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta tgagcgtttc
    14941 atcatttcga agaaatcgtc attggtcttg gtcatggcca gtttattaat gaggaattcc
    15001 attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat tttctgcagt
    15061 tcttcctgag tcgtgagcag ctcttcttta cgggtaccgg aacggttgta gtcgatagcc
    15121 gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt gcctgtacct
    15181 ttaaactctt cgtaaataac ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg
    15241 ataatggtca ggctgccgcc ctcttccacg ttacgcgccg caccgaagaa gcgtttcgga
    15301 cgatgaaggg cattggcgtc cacaccaccg gtcaacactt tacctgacgc cggaacaaca
    15361 gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac gtctttcttg
    15421 tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac gtggcgagat
    15481 gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg catctcggtt
    15541 acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg gtggttgtaa
    15601 gcaatgctct gagcaatgtt ctgcagcagc atagttttac cggctttcgg cggtgccaca
    15661 atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg agcggttaaa
    15721 tcttccgtag aaccgttacc acgttccata cgcagacgag agtttgcgtg cagcggggtt
    15781 aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta
    15841 actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt accagagatg
    15901 gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta gatgtcatca
    15961 ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc ctgcaatatc
    16021 tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt caggatggca
    16081 aaaataatgt cctgcttacg catacgagcc aggttttcca gccccatatt ttcgccgaga
    16141 gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt ggtgtgagtt
    16201 cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg atcatccatg
    16261 cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga gtacgcagaa
    16321 ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg catttaagat
    16381 aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt taatacgaag
    16441 tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg gggcacccga
    16501 acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga cctttagaca
    16561 gtgcacccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac
    16621 cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg
    16681 tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg
    16741 gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg
    16801 tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac tccacaggaa
    16861 taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag gttgacttta
    16921 tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac actatgagca
    16981 aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag gttgtagaag
    17041 tccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc cttccgctga
    17101 cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt
    17161 ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat cgcaaggtga
    17221 atcaaccacg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag atccatgccg
    17281 acgcagaacc gctggcggaa gctaccggcc tgaagctggg tctggcttac ggtggtgatg
    17341 gctacgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt ggcactacgg
    17401 ggcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt caggtggtgg
    17461 tactggacga agccgatcgc atgtacgatc tgggctttat taaagatatc cgctggctgt
    17521 tccgccgtat gccgcctgca aaccagcgcc tcaacatgct gttctccgcc acgctttcgt
    17581 accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac
    17641 cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct aacgaagaaa
    17701 aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg attattttcg
    17761 ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat ggtcatcgtg
    17821 tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta
    17881 cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc
    17941 cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat tacgttcacc
    18001 gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag
    18061 agtatgcatt gaatttgcct gctattgaga cctatattgg tcactcaatt ccggtaagca
    18121 aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc acgcgcccac
    18181 gtacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa
    18241 taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa tgaatcccga
    18301 tgagcatact gtagtaagtg attcaggtga aagaatgcag ccaacacaga gacagattga
    18361 aggatgaaga gtatgggttc catctcgtcg ctgtatgcag ccattgatct cggttcgaat
    18421 agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct gacgcgaata
    18481 aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgccctgtc taatgaagca
    18541 atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga tatccctccc
    18601 tcgcaaattc gcgttgtcgc tacggcgaca ttacgtcttg ccgtcaatgc gggtgatttt
    18661 attgccaaag cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa
    18721 gcacgtctga tttatcaggg cgttgctcac acaactggcg gtgccgatca gcgcctggtg
    18781 gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca aaccacctcg
    18841 ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc cgatcgtaat
    18901 ctggggcagg aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt
    18961 gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag
    19021 gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct ggaaaagttg
    19081 cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga gattgacggg
    19141 ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat cgccattttt
    19201 accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc
    19261 tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacgct gcgtaatatt
    19321 cagcgccgct ttatgatcga tattgatcag gcgcagcgcg tagccaaagt tgcggctaac
    19381 ttcttcgatc aggtggaaaa ggaatggcat cttgaagcaa taagccgcga tttgctcatc
    19441 agcgcctgtc agcttcatga aatcggcctg agcgttgact ttaaacaagc gccgcaacac
    19501 gctgcttatc tggtgcgtaa tctggatctt cccggtttta cccccgcaca gaaaaaattg
    19561 ctggcgacgc tactgctcaa ccagactaat ccggtcgatc tctcatcgct gcatcagcaa
    19621 aatgccgtac cgccgcgcgt cgcagaacaa ctctgccgtt tactccgcct ggccatcatt
    19681 tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc taaccatgaa
    19741 ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg taaagagatt
    19801 attgctcagg aaagccagtg gcagagctat gtccactggc cgctggaagt gcattaatga
    19861 gtaagtgccg gatgcgatgc tgacgcatct tttccggcct tgattatttc cctcgttttg
    19921 ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc cgttcttcgg
    19981 cgctgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa
    20041 agcggctcgg ctccgggcgc accagctcgc cgtactgacg gcgttcttta cacagcgtaa
    20101 aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc tcctcatcga
    20161 tattatcttc atcgatgctg ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt
    20221 agacataagg aaactccagc ccttttgacg cgtgcagagt catcagttgc acctgatcca
    20281 gctcttcttc actctcacca cgctccatca tgtcgcgcaa agtaaagcgc gtcaccacct
    20341 gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc atccagctaa
    20401 acagttggtt gacgttcttc atgcgcattt cggcggcttt cgggctgggc gatgtttcgt
    20461 acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca atcggctccc
    20521 gctccgccag acgctggatt tctgccaacc agtgagtaaa gcgggtcaat gcttcataac
    20581 cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac atgcttttat
    20641 tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc tctctcttcg
    20701 gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc acgcgcagat
    20761 aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca gaaattttgt
    20821 acgggatgcg gttttgcatc aggaattttt caaacactcg cgactgatgg ttaccgcgat
    20881 aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca atcagttcac
    20941 cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaacttta agctctgtgc
    21001 cgtaacccag ttcggagaac agacgcttct caaagacgtg cgggttattg gcgatcagga
    21061 tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc ttaatcacct
    21121 tcagcgccgg aaaatcctga ctcagcagca ccaggttttg tggacgtgcg ccgcgccagg
    21181 agtagatcga ctggtcgtca tcgcccacca cggtaaagcg cgcgcggctg cccaccagca
    21241 gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ctcatccacc agcagatagc
    21301 gaattttgtt ctgccagcgc tcacggactt cttcattgcg ttgcagcagc aacgtcggca
    21361 ataaaatcag atcatcgaag tcgagaacat tgcacgcttt caggtgtgca tcatacagcc
    21421 cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc gcctgggacg
    21481 gtgttttgag atcgttcttc cagttagaga tggtcgaaat caactgttgc aggagaactt
    21541 tgtcatcttc aatcagccct tcggtcaact ctttgagcaa cgccagctga tcggtatcgt
    21601 caaacagcga aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg atgatatcca
    21661 gccccagcgt gtggaaggtt gagatcatca gcccacgcgc ctctttgcga cccagcgtct
    21721 gtccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat
    21781 gtcgcgcctg ataaccgcaa ccgcggatca gatgggcgat tttattggtg atcacacgag
    21841 ttttaccgga acccgcgccc gccagcacca ggcaggggcc ggtaacgaat tcgacagctt
    21901 gttgttggcc ggggtttaga cgcataggtg tattgctcaa tcttcgaacg gggggaggat
    21961 tgtagcagaa tcgcatttga atgtgaccgc gtgtaagccg ctaaccggtt ggctgcggat
    22021 gtttaaatca gagagggaag accgaaaccg gagttcatcg tgaatccatt agacttagaa
    22081 aatatcgggt cgtaacgttg tgttattact ttttcagagt ttactaagga gaggtgtact
    22141 cgtggcgatt aatttcagtc ctaaggttgg tgaaatactg gaatgcaact ttggtaacta
    22201 tcctgttagc cagaatggac agttcagcac cacgtattac gatggccgca ttcctcctga
    22261 aatgataaaa aacaggtta
//
LOCUS       DADLTD010000053        21346 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLTD010000053 DADLTD010000000
VERSION     DADLTD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.053"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            143..346
                     /locus_tag="JN701_001589"
     CDS             143..346
                     /locus_tag="JN701_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY7364079.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            354..1286
                     /gene="aaeA"
                     /locus_tag="JN701_001590"
                     /pseudo
     CDS             354..1286
                     /gene="aaeA"
                     /locus_tag="JN701_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            1292..3259
                     /gene="aaeB"
                     /locus_tag="JN701_001591"
     CDS             1292..3259
                     /gene="aaeB"
                     /locus_tag="JN701_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY7364080.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3351..3623
                     /locus_tag="JN701_001592"
     CDS             3351..3623
                     /locus_tag="JN701_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364081.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3679..3942)
                     /gene="yhcN"
                     /locus_tag="JN701_001593"
     CDS             complement(3679..3942)
                     /gene="yhcN"
                     /locus_tag="JN701_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY7364082.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(4307..4777)
                     /gene="argR"
                     /locus_tag="JN701_001594"
     CDS             complement(4307..4777)
                     /gene="argR"
                     /locus_tag="JN701_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY7364083.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            5212..6150
                     /gene="mdh"
                     /locus_tag="JN701_001595"
     CDS             5212..6150
                     /gene="mdh"
                     /locus_tag="JN701_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY7364084.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(6213..7280)
                     /gene="degS"
                     /locus_tag="JN701_001596"
     CDS             complement(6213..7280)
                     /gene="degS"
                     /locus_tag="JN701_001596"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY7364085.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(7370..8737)
                     /gene="degQ"
                     /locus_tag="JN701_001597"
     CDS             complement(7370..8737)
                     /gene="degQ"
                     /locus_tag="JN701_001597"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY7364086.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(8891..9289)
                     /locus_tag="JN701_001598"
     CDS             complement(8891..9289)
                     /locus_tag="JN701_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY7364087.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            9483..10610
                     /locus_tag="JN701_001599"
     CDS             9483..10610
                     /locus_tag="JN701_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY7364088.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            10828..11256
                     /gene="rplM"
                     /locus_tag="JN701_001600"
     CDS             10828..11256
                     /gene="rplM"
                     /locus_tag="JN701_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY7364089.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            11272..11664
                     /gene="rpsI"
                     /locus_tag="JN701_001601"
     CDS             11272..11664
                     /gene="rpsI"
                     /locus_tag="JN701_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY7364090.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            12059..12697
                     /gene="sspA"
                     /locus_tag="JN701_001602"
     CDS             12059..12697
                     /gene="sspA"
                     /locus_tag="JN701_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY7364091.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     IHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            12703..13200
                     /gene="sspB"
                     /locus_tag="JN701_001603"
     CDS             12703..13200
                     /gene="sspB"
                     /locus_tag="JN701_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY7364092.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(13243..14609)
                     /gene="dcuC"
                     /locus_tag="JN701_001604"
                     /pseudo
     CDS             complement(13243..14609)
                     /gene="dcuC"
                     /locus_tag="JN701_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            14989..15780
                     /gene="nanR"
                     /locus_tag="JN701_001605"
     CDS             14989..15780
                     /gene="nanR"
                     /locus_tag="JN701_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY7364093.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            15902..16795
                     /gene="nanA"
                     /locus_tag="JN701_001606"
     CDS             15902..16795
                     /gene="nanA"
                     /locus_tag="JN701_001606"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY7364094.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            16904..18394
                     /gene="nanT"
                     /locus_tag="JN701_001607"
     CDS             16904..18394
                     /gene="nanT"
                     /locus_tag="JN701_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY7364095.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            18442..19131
                     /locus_tag="JN701_001608"
     CDS             18442..19131
                     /locus_tag="JN701_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY7364096.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            19128..20003
                     /locus_tag="JN701_001609"
     CDS             19128..20003
                     /locus_tag="JN701_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY7364097.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            20000..20464
                     /locus_tag="JN701_001610"
     CDS             20000..20464
                     /locus_tag="JN701_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7364098.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(20540..20845)
                     /locus_tag="JN701_001611"
     CDS             complement(20540..20845)
                     /locus_tag="JN701_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7364099.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(20862..21341)
                     /locus_tag="JN701_001612"
     CDS             complement(20862..21341)
                     /locus_tag="JN701_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY7364100.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT         5149 a         5291 c         5524 g         5382 t
ORIGIN      
        1 agataacagc ataacagtta cgctaatata ttaaatatca atctacagca atgttgctct
       61 cgcccggctt tccatgccct tctctgcggc gacagatgct gaaaataata acgcctgctc
      121 tctctataca accaaggtca acatgagtct gtttcccgtt atcgtggtgt ttgggctgtc
      181 cttcccaccg atattttttg aattgctttt atcactggcg attttctggc tggtgcgccg
      241 ggtacttgtg ccaacaggta tctacgactt tgtctggcat ccggcgttgt tcaacaccgc
      301 gctctattgc tgcttgtttt atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa
      361 cactaataag aaaattctcc cgtacggcca tcacggtcgt attagtcatt ctggccttca
      421 tcgcaatttt taatgcctgg gtctattaca ccgaatcccc ctggacgcgt gacgcgcgct
      481 ttagcgctga cgtcattgcg atcgcgccgg acgtttctgg actcattacc caggtgaatg
      541 ttcatgataa ccagctggtg aaaaaaggac agatactgtt caccatcgac cagccgcgct
      601 atcaaaaggc gcttgaggaa gcgcaagccg atgttgctta ttatcaggta ctggcacagg
      661 agaaacgcca ggaggccgga cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag
      721 agatcgacta ggctaacaac gtactacaaa cggttctgca tcagttagcg aaagcgcagg
      781 cgacccgcga tctggcaaaa ctggatcttg aacgcacggt gatccgcgcg ccagcagatg
      841 gctgggtgac caacctcaac gtctataccg gtgagtttat tactcgagga tcaacggcgg
      901 ttgcgctggt gaaacagaac tccttctatg tactggccta tatggaagaa actaagctgg
      961 aaggggtgcg tccggggtat cgtgcagaga tcacgccgct tggcagtaac aaagtgctga
     1021 aagggactgt tgatagtgtt gccgcagggg tcaccaacgc cagcagcacg cgtgacgaca
     1081 aagggatggc gactatagac tctaaccttg aatgggtgcg tcttgcgcaa cgtgttccgg
     1141 ttcgtattcg tctcgacaac cagcaagaga acatctggcc tgcgggcacc actgctacag
     1201 tggtggtcac tggcaaacaa gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg
     1261 cccatcgcct gcgtgagttt ggttaatcac gatgggtatt ttctccattg ctaaccaaca
     1321 tattcgcttt gcggtaaaac tggcgaccgc cattgtactg gcgctgtttg ttggctttca
     1381 cttccagctg gaaacgccac gctgggcggt actgacagcg gcgattgttg ccgccggtcc
     1441 ggcctttgct gcgggaggtg aaccgtattc tggcgctatt cgttatcgtg gctttttgcg
     1501 catcatcggc acatttattg gctgtattgc cggactggtg atcatcattg cgatgatccg
     1561 cgcaccatta ttgatgattc tggtgtgctg tatctgggcc ggtttttgta cctggatatc
     1621 ctcgctggta cgaatagaaa actcgtacgc gtgggggctg gccggttata ccgcgctgat
     1681 cattgtgatc accattcagc cggaaccatt gcttacgccg cagtttgccg tcgaacgttg
     1741 tagcgagatc gttatcggta ttgtgtgtgc gattatagcg gatttgctct tttctccgcg
     1801 atcgatcaaa caagaagtgg atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt
     1861 aatgcaactc tgtatcaagc atggcgatgg tgaagttgtc gataaagcct ggggcgacct
     1921 ggtgcgacgc accacggcgc tacaaggcat gcgcagcaac ctgaatatgg aatcttcccg
     1981 ctgggcgcgg gccaatcgac gtttaaaagc gatcaatacg ctatcgctga cgctgattac
     2041 ccaatcctgc gaaacttatc ttattcagaa tacgcgcccg gaattgatca ctgatacttt
     2101 ccgcgaattt tttgacacgc cggtagaaac cgcgcaggac gtccacaagc agctcaaacg
     2161 cctgcggaga gttatcgcct ggaccgggga acgggaaacg cctgtcacca tttatagctg
     2221 ggtcgcggcg gcaacgcgtt atcagcttct caagcgcggc gttatcagta acacaaaaat
     2281 caacgccacc gaagaagaga tcctgcaagg cgaaccggaa gtcaaagtag agtcagccga
     2341 acgtcatcat gcgatggtta acttctggcg aaccacactt tcctgcattc tgggcacgct
     2401 tttctggctg tggacgggct ggacttccgg cagtggtgca atggtgatga ttgcggtagt
     2461 gacgtcactg gcaatgcgtt tgccgaatcc acgcatggtg gcgatcgact ttatctacgg
     2521 gacgctggcc gcgctgccgt tagggctgct ctactttttg gtgattatcc ctaataccca
     2581 acagagcatg ttgctgctgt gtattagcct ggcagtgctg ggatttttcc tcggtataga
     2641 agtacagcaa cggcgactgg gctcgatggg ggcgctggcc agcaccataa atattatcgt
     2701 gctggataac ccgatgactt tccatttcag tcagtttctc gacagcgcat tagggcaaat
     2761 cgtcggctgt gtgctcgcgt tcaccgttat tttgcttgtg cgggataaat cgcgcgacag
     2821 gaccggacgt gtactgctta atcagtttgt ttctgccgct gtttccgcga tgactaccaa
     2881 tgtggcacgt cgtaaagaga accacctccc ggcactttat cagcagctgt ttttgctgat
     2941 gaataagttc ccaggggatt tgccgaaatt tcgcctggcg ctgacgatga ttatcgcgca
     3001 ccagcgcctg cgtgatgcgc cgatcccggt taacgaggat ttatcggcgt ttcaccgaca
     3061 aatgcgccgc acagcagacc atgtgatatc tgcccgtagc gatgataaac gtcgtcggta
     3121 ctttggccag ttgctggaag aacttgaaat ctaccaggaa aagctacgca tctggcaagc
     3181 gccaccgcag gtgacggaac cggttcatcg gctggcgggt atgctccata agtatcaaca
     3241 tgcgttgacc gatagttagg tcaaaaccga cgccaaaagt gtcggttttt tcatggctat
     3301 acttagcgat gcacggcaga actcgccgcg aaacgtgacg gtggcaatag atgaatattt
     3361 atacctttga ttttgatgag attgagagtc aggaggattt ttatcgtgac tttagccaaa
     3421 cctttggtct ggcgaaagat aaggtacgcg atctcgactc actatgggat gtgttaatga
     3481 acgatgtcct gccgctacca cttgagattg aatttgttca tctgggagag aaaacgcgtc
     3541 gccgttttgg cgcgttaata ttgctgtttg atgaggcaga ggaagagctg gaagggcatt
     3601 tgcgttttaa tgttcgtcat tagcgcaaaa aaaagccccc gaaccggggg caatatcgtc
     3661 ggacaagacg atgagggttt atttgtacag ttcagccgta gcgtgccagg tgtcaccgct
     3721 acgagcttca gtaatctggt aggccgttgc gcctttctct tccgcttttt tgttcagcat
     3781 ttcacgcata tccattggcg aagacgccac accacttacg gatacggtcc cgattgcttc
     3841 acgattttgt gcttgtgcag catcaatgga gtcggcagcg aatgcaccga aagagagaac
     3901 agaaagtacg cttaatgcag ctacagtggt tttgattttc atgattttta cctcgacata
     3961 atcttttagc tgggcctttg ttttgtgacc catatcacaa aatcaagcat acactaatta
     4021 ttgaactaat taataccacg ctaactgttt cagttgatac taagtgttaa aaatgtgaat
     4081 tttaataaca aaaaagaatt aaaaacacgg tcatttattt ttaattctag tattttcaat
     4141 tagataatga attttgcata atgtgctttt tttaccgtat gtattcatta tgtgaatgac
     4201 atgtcgcagt aaaacgcact attcgttaaa ttaatcgcag gggaaagcag ggggttgaga
     4261 gggataagca acattttccc cgccgccaga agcgacgggg cagagattaa agctcctggt
     4321 cgaacagctc taaaatcgct tcgtacaggt ctttgacggt gaaaccgtta gcaggggtgg
     4381 taaagatggt gtcatcgcca gcgatggtgc ccagaatacc ttctgctttg cccagtgagt
     4441 ccagcaggcg agcaattaac tgcgccgcgc cagggctggt atgaatcacg acaactgcat
     4501 cgttgtagtc gatatccagc accagattct tcaatggact ggaggtggtt ggtacaccca
     4561 gttcagctgg caggcagtaa accatttcca ttttggcatt gcgtgtacgt acagcaccaa
     4621 acttggtcaa catccgcgag actttagact gattaatatt gtcaaagcct tgctcctgca
     4681 acgcggcgac gatttcgccc tgggagctaa atttctcttc tttaagtaat gctttaaatg
     4741 ctttaactag ttcttcttgc ttagccgagc ttcgcataag tcacccgata tggtggttga
     4801 tacaacatta ttgtgcatac agatgaattt ttatgcaaac agtcaaccct gaagaaggct
     4861 gaaataatgt tatgaaagag cgggatttta tcaaatttcg ttattgagaa acatgcctgc
     4921 gtcacggcat gcaaattctg cttaaaagta aattaattgt tatcaaatta atgttgtttt
     4981 ggctgaacgg tagggtatat tgtcaccacc tgttggaatg ttgcgctaat gcataagcga
     5041 ctgttaatta cgtaagttag gttcctgatt acggcaatta aatgcataaa cgctaaactt
     5101 gcgtgactac acattcttga tatgtggtca ttgtaaacgg caattttgtg gattaaggtc
     5161 gcggcagcgg agcaacatat cttagtttat caatataata aggagtttag gatgaaagtc
     5221 gcagtcctcg gcgctgctgg cggtattggc caggcgcttg cactactgtt aaaaacccaa
     5281 ctgccttcag gttcagaact ctctctgtat gatatcgctc cagtgactcc cggtgtggct
     5341 gtcgatctga gccatatccc tactgctgtg aaaatcaaag gtttttctgg tgaagatgcg
     5401 actccggcgc tggaaggcgc agatgtcgtt cttatctctg caggtgtagc gcgtaaaccg
     5461 ggtatggatc gttccgacct gtttaacgtt aacgccggca tcgtgaaaaa cctggtacag
     5521 caagttgcga aaacctgccc gaaagcgtgc attggtatta tcactaaccc ggttaacacc
     5581 acagttgcaa ttgctgctga agtgctgaaa aaagccggtg tttatgacaa aaacaaactg
     5641 ttcggcgtta ccacgctgga tatcattcgt tccaacacct ttgttgcgga actgaaaggc
     5701 aaacagccag gcgaagttga agtgccggtt attggcggtc actctggtgt taccattctg
     5761 ccgctgctgt cacaggttcc tggcgttagt tttaccgagc aggaagtggc tgatctgacc
     5821 aaacgtatcc agaacgcggg tactgaggtg gttgaagcga aagccggtgg cgggtctgca
     5881 accctgtcta tgggccaggc agctgcacgt tttggtctgt ctctggttcg tgcactgcag
     5941 ggcgaacaag gcgttgtcga atgtgcctac gttgaaggcg acggtcagta cgcccgtttc
     6001 ttctctcaac cgctgctgct gggtaaaaac ggcgtggaag agcgtaaatc tatcggtacc
     6061 ctgagcgcat ttgaacagaa cgcgctggaa ggtatgctgg atacgctcaa gaaagatatc
     6121 gccctgggcg aagagttcgt taataagtaa ttgattagcg aataataaaa aaccggagca
     6181 cagactccgg ttttttgttt tgagcactcg acttaattgg ttgccggata ttcctgaatg
     6241 gtgacctgca gcgttaactg cttatcatca cgcatcacta caaccgggat caccgaacca
     6301 gggcgaattt ctgccacctg atccatcgtc tccagagcag agatggccgg tttgttatcc
     6361 accgaaataa tcagatcgtt gacctgaata cccgcattcg ccgccgggcc gtcaggtgac
     6421 acttcattaa ccacgatccc ttgcagttga tctataccac cgctctgcgc gtgcagtggt
     6481 gcgatctcac gtccaccaat gccaatgtag ccgcggatca cgcgcccatc gcggatcagc
     6541 ttatccataa ttttggttgc taactggaaa ggaatcgcaa agccgatacc ttccggcgtt
     6601 tcgccatcgt tactcttatc aaacgacagc gtgttaatgc ccatcagttc tcccagcgag
     6661 ttcaccagcg cgccgccaga gttaccgtgg ttaatggaag catcggtttg taggaagttt
     6721 tgccgcccgg tagggttcag accgattcgc cccgtggcac taataatccc ttgggtaatg
     6781 gtctgcccga ggttgtacgg gttaccgatc gccagtacta cgtcgccaat gtgcggtacg
     6841 cgacgtgcat taattggaat ggtaggtaaa ccgccagtgg cattaatttt aagtaccgcc
     6901 agatcggtta gagagtcaga tcccaccagc aatgcttcaa atacacgtcc atcctgtaag
     6961 gcgacgatga tctgatcggc gtcgttgatg acgtgtttat tggtgatgat ataaccgcgt
     7021 tgatccatga ttacaccgga tcccagggtg cggatctcaa gctggttgtg agagttggtg
     7081 ttcacaccac ggttgtaaac gttaaccacc gctggcgcgg cgcggcgaac cgccagatta
     7141 tagctggcag gcgtctcatc ggtactgtca aattgcggag tggaaagcgg gttaaggctg
     7201 cgcagcgaag gcatggcaac cagcagaata gcgccgacaa ttaatccaat cgcaacggaa
     7261 cgtaagagct tcacaaacat gatggaggcg tcattaaaaa agggaacggc agcagcatac
     7321 cacgagttaa ccggacatca cacgtaagcc tgatgcccgg tttacgacat taacgcatca
     7381 gcagatagat gctttcattg ccgcgtacaa tttgcagggc gatgatggcc ggttttgccg
     7441 ccagcacttt acgcatttca gcaatcgagt tcacccgatc gcggttgacg ccaatgatca
     7501 catcgtcttt ttgcaagcct gcctgagcag ctgggcttcc tttgacaact tcatcgatct
     7561 taataccttt gccgcaatct tttagctgac catcgctcaa cgttgcacct tccagcgctg
     7621 gcgtgatcat ttcagcgctg gccgacgaag aggtgctggt atcaagcgtc acttctactt
     7681 ccagtggttt gccgttacgc agcaggccaa gcttcacttt cgtgcccggc tctgtagtcg
     7741 cgatacgaga gcgcaactca gcaaagctat tcagcggttt gccgttgagg ctggtaataa
     7801 tatcgcccgc tttgacgccc gcttttgccg agccagaacc tggcaacact tcgctgacaa
     7861 acgcgccacg ctgcacgtca aggttgaagg ctttggcgat atcggcactc atctcggtgc
     7921 ctttgatgcc taacaaaccg cgtttgattt caccaaagtc gataagctgc tgcgccagtg
     7981 ttcgcgccat attactgggg atggcaaatc caatcccgac gctcccgccg ccaggcgcaa
     8041 ggattgctgt gttgatgcca attaactcac cgttaaggtt taatagtgca ccgccggagt
     8101 taccgcggtt aatggaagca tctgtctgga taaagttttc cagaccttca agattcaacc
     8161 cgctgcggcc taatgcggaa acaatgccag aggtggcggt ttgcccaagg ccaaatgggt
     8221 taccgaccgc tacggcaaaa tcaccgacgc gcaatttatc ggagtcggca atagcgattt
     8281 gcgttaattt gctcgggttt tgaatttgta acagggcgat atcgctctgg tcatcgctac
     8341 caatcagttt tgcatcaaac tcgcgcccat cattgagctg aatactgatt ttctgtgcct
     8401 gattaatcac atggttgttg gtcagcacat agcctttact ggcgttgatg atgacaccgg
     8461 aacctaaacc ttcgaagggt tgtgcaggtt gatccggtaa atcatcacca aaaaactttt
     8521 tgaattcttc cgggattttc tgtccctgac tggccgttcc ttccacccgt acgctcacca
     8581 ctgccggaag cactttttcc agcattggag ccagactggg gagaggggcc tgatcggcaa
     8641 cctggcctgg aatcgacgcg acggcctgaa atgacgccga gagagttaac ccgacactta
     8701 acgctaatgc actcaacagc tgggtttgtt ttttcattat tcctgctctc gtacctgaat
     8761 gataagaaaa gagattcaaa acagtttgat gttattgaat tttcagacgg ctaacaatga
     8821 gacaccagat taaatgatag tcggggaggg agagaagagg agggcgcaat ggctgcgccc
     8881 gaaaaataaa ttagtcgcgc ttcgcgccag tacgcagcag gccggatgca ccttcagaat
     8941 agtcgcgagg catctgcacc ggtgcctgat cgttgctggc ttcagactct gccagacgat
     9001 tacggaacgg gtttgcttca gcagacagtt ccggcagcag gctgctggag cttttcgcca
     9061 tgtgctgata cagctggcga tagtcgtgcg ccatggtatc cagtaattcc gcgctgcggg
     9121 caaagtggct aaccagctct tcgcgatact cgtccagttc agctttattc ttttccagtt
     9181 cgtactgcaa cgcctgttgc tggcgtagtt tacgattacc aaaacgcatg gccacagcac
     9241 caataatgat gccgacgact aacccaatta gcgcatattc ccaggtcatg aacatctccc
     9301 gttgtctttt gtttccgtag ggtgttggct tcaggctcca tgcctacggc tgattatgcc
     9361 actataaccg ttaattccac agaagtggaa tcccgactgc atatcgcgta gtgtagaacg
     9421 gccttttttt cgtcaaccgt gaacaacggc gcaccgatta ttcaaggaat aataataaga
     9481 tcatgcaaag cgttacccca acatcgcaat acctgaaggc gctcaatgaa ggcagccatc
     9541 aacccgacga cgttcaaaaa gaggccgtca gccgcctgga aattatttat caagaactca
     9601 tcaatagcag gccaccagcc cccaggacga gtgggctaat ggcgcgggtc ggtaagctgt
     9661 ggggtaaacg cgaagacaca aagcatacgc cagtgcgtgg cttatatatg tggggcggtg
     9721 taggacgcgg gaaaacctgg ctgatggacc ttttctatca aagcctgccg ggagagcgga
     9781 aacagcgcct gcactttcac cgttttatgc tgcgggtgca cgaagagcta actgccttac
     9841 aggggcagac cgatccgctg gaaattattg ccgatcgctt taaagccgaa actgacgtgc
     9901 tctgttttga cgaatttttt gtttctgata ttaccgacgc catgctactt ggcggtctga
     9961 tgaaagccct gttcgcccgc ggtattaccc tggtagcaac gtcaaatatt ccgccggacg
    10021 aactttatcg aaatggcttg caacgtgcgc gttttctgcc tgcaatcgat gccattaaac
    10081 agcattgtga tgtaatgaac gtggacgctg gtgttgatta tcgactgcgt acactcactc
    10141 aggcgcatct gtggctttcg ccactccacg atgaaacccg ggcacaaatg gataaactat
    10201 ggttggcgct ggcgggagcg aaacgcgaaa attcaccgac gttagaaatc aaccatcgac
    10261 cgctggcgac aatgggcgct gagaatcaga cgcttgctgt ctcttttacc accttgtgtg
    10321 tcgatgcccg cagccagcat gactatattg cgctctcacg cctctttcat acggtcatgt
    10381 tgtttgatgt accagttatg acgcggttga tggagagcga agcgcggcgc tttattgcgc
    10441 tggtggatga gttttacgag cgccatgtca aattagtggt gagtgcagaa gtgccgctgt
    10501 atgaaattta tcagggcgat cggctgaagt ttgagttcca gcgttgcctg tcacgtctgc
    10561 aagagatgca aagcgaagag tatctgaagc gcgagcattt ggcgggttaa aacctgtcac
    10621 aaatcacaaa aaggggtcga tctttgaccc cgacttctct ataatcctgc gaccccacgt
    10681 tacaagaaag ttttttccca aaactttttg tgtgctggca taggctattc gaaggggtag
    10741 gtttgccgga ctttgtcgtg tgaacctcaa caattgaaga cgtttgggtg ttcaccaacg
    10801 tgtaactatt tattgggtaa gcttttaatg aaaactttta cagctaaacc agaaaccgta
    10861 aaacgcgact ggtatgttgt tgacgcgacc ggtaaaactc tgggccgtct ggctactgaa
    10921 ctggctcgtc gcctgcgcgg taagcacaaa gcggaataca ctccgcacgt agataccggt
    10981 gattacatca tcgttctgaa cgctgacaaa gttgctgtaa ccggcaacaa gtgtactgac
    11041 aaagtgtact atcaccacac cggccacatc ggtggtatca aacaagcgac ctttgaagag
    11101 atgattgctc gccgtcctga gcgtgtgatt gaaatcgcgg ttaaaggcat gttgccaaaa
    11161 ggcccgctgg gtcgtgctat gttccgtaaa ctgaaagttt acgcaggtaa cgagcacaac
    11221 cacgcggcac agcaaccgca agttcttgac atctaatcgg gattataggc aatggctgaa
    11281 aatcaatact acggcactgg tcgccgcaaa agttccgcag ctcgcgtttt catcaaaccg
    11341 ggcaacggta aaatcgtaat caaccaacgt tctctggaac agtacttcgg tcgtgaaact
    11401 gcccgcatgg tagttcgtca gccgctggaa ctggtcgaca tggttgagaa actggacctg
    11461 tacatcaccg ttaaaggtgg tggtatctct ggtcaggctg gtgcgatccg tcacggtatc
    11521 acccgcgctc tgatggaata cgacgagtcc ctgcgttctg aactgcgtaa agctggcttc
    11581 gttactcgtg acgctcgtca ggttgaacgt aagaaagtcg gtctgcgtaa agcacgtcgt
    11641 cgtccgcagt tctccaaacg ttaattggct tctgctccgg cagaaaacaa ttttcgaaaa
    11701 aacccgcttc ggcgggtttt tttatagcta aaatctgaat cagcgtaaaa actggaaagt
    11761 tgctttttgc tgccacctga cagacaggta aaacaaaaac cacatcgcca ataagggact
    11821 aagtcaacta tttcagacta aagcgcatct ctttttcccc atttccggca tcgactcacc
    11881 acaaaggtcg caaaatctgg tagactatca tccaattttc tgcccaaatg tcgggtattg
    11941 ctcatttttt gtttgatttt cgaacaaaga gagtcgttcc ttactgggta acacaacttc
    12001 tgactggcca cctggtggct ggtagcagta aaaattctga ctatacctgg aggttttcat
    12061 ggctgtcgct gccaacaaac gttcggtaat gacgctgttt tccggtccta ctgacatcta
    12121 tagccatcag gtccgcattg tgctggctga gaaaggtgta agtttcgaga tcgaacacgt
    12181 ggaaaaggac aatccgcctc aggatctgat tgacctcaac ccgaatcaga gcgttccgac
    12241 cctggtggat cgtgagctga ccctgtggga atctcgcatc attatggaat atctggatga
    12301 gcgtttcccg catccgccac tgatgcctgt ttacccggta gctcgcggtg aaagccgtct
    12361 gtacatacat cgcatcgaaa aagactggta cacgctgatg aacaccatca tcaacggttc
    12421 agcttctgaa gcagatgccg cacgtaagca actgcgcgaa gaactgctgg cgattgcgcc
    12481 ggtcttcggt cagaagccgt acttcctgag cgatgagttc agcctggtcg attgctatct
    12541 tgctccgctg ctgtggcgtc tgccgcaact gggcatcgag ttcagcggcc cgggtgcgaa
    12601 agagctgaaa ggctatatga cccgcgtctt tgagcgtgac tctttccttg cttctttaac
    12661 tgaagcagaa cgtgaaatgc gtctgggccg gagttaatct gtatggattt gtcacagcta
    12721 acaccacgtc gtccctatct gctgcgtgca ttctatgagt ggttgctgga taaccagctc
    12781 acgccgcacc tggtggtgga tgtgacgctc cctggcgtgc aggttcctat ggaatatgcg
    12841 cgtgacgggc aaatcgtact caacattgcg ccgcgtgctg tcggcaatct ggaactggcg
    12901 aatgatgagg tgcgctttaa cgcgcgcttt ggcggcattc cgcgtcaggt ttctgtgccg
    12961 ctggctgccg tgctggctat ctacgcccgt gaaaatggcg caggcacaat gtttgagcct
    13021 gaagctgcct acgatgaaga taccagcatc atgaatgatg aagaggcatc ggcagacaac
    13081 gaaaccgtta tgtcggttat tgatggcgac aagccagatc acgatgatga cactcatcct
    13141 gacgatgaac ctccgcagcc accacgcggt ggtcgaccgg cattacgcgt tgtgaagtaa
    13201 tacaaaaaca ggccctggtg gcctgttttg tcttttaatg gcttaataaa atagcgtgat
    13261 tgtggcaatc atattcacca cgaaaccgac tgccatgggg atcgctgttc gcttcaccac
    13321 cgcaaaaggc gaaacgcctg caattcctga cgtaacgacc accaccgcag taatcggcga
    13381 aaccgcgcgc gctagcgtcg tggcaaaatg catcggcata atcattacaa ccgctggtac
    13441 atgtagtccg gctgcgatat tcggaataag actggcaaat gacataaacg gcgcattgcc
    13501 agagcccatc acaatggcac aaatggcaat gaccagcgcc ataataatca tcacgccaat
    13561 accgcccaga ccagaatgct ccgcacccct gataaccgca tcgacagtgc caatcgtcgt
    13621 taagcctttc gcaaatattt cacccgcgac caccagcgtt accacgttgg caaactgcgt
    13681 acccatgccg tcaaaaaacg cctgcacatc gtccattgtt tcgcgcaagt tatgcttgcg
    13741 gaagaactca acaaacatcg tcaccgtcaa actcagtaac atcaccacca ccagatgcag
    13801 ttctgactgc atcagcccga cgtgggcgag gaacagcgag ccgagcatca ggattaacgg
    13861 catcacaggt aaaatggcgt aatagagcgg cgggacatta tcgagagctt tttgctctgc
    13921 ttgttcgtga ttgatatttt tatctttttt gtcaaaagcg cgttgcacga aaaagtggga
    13981 gatcgccacc gagataatga cgcaagaggc gaccggaagc tggtagtgga agaagtaagt
    14041 ggcaattttc attcccgcga cctgggctgc aaaaatggag ttcgtttcca gaattcccca
    14101 ctcaatggac attgtggttg cgataaccgc caccgcagag agacgactta ctcccagact
    14161 caccagtgtc ggaaataagg tgaccatcag caacataccc agaccggagg cgctggtaat
    14221 aaactgcgcc atgatttgac cgatgacgta agttgccgac agaataatat acggtgagcg
    14281 aatgagtttt aacgggcggc ttaacaagct caccatcgcg cgactggccc ctatgcgctc
    14341 catgtagcgg gcataccgcc caccgccata atcgatagcc ccaatccggc aatgcggttg
    14401 gacagcatgc gcaggatttc attataaata tcgacgatca agtagccgct gcttttggcg
    14461 ggctcgagta caccaccgaa ccctaaccag acaccgcaca tcatcaggaa gatacctgcg
    14521 gcagccagca ccacctgagg tttgtagttt ttcaggatca aatagcccat cgtaattaat
    14581 acgatgacag atataattat gccgaacatt gttgaactcc gtgtcaaaag aaaacggtca
    14641 atcccataaa cggcagattg aaaacaatga tgttatattt tttgcaagtc tatttatggt
    14701 gcggatgtcg tgtttttaat tgtaggtgag ataatttttc attaaaaaat atgcgcttat
    14761 gattattttg caagaacgca ttcataatat tcataatgct cgtgaatagt cttataaata
    14821 attcaaacgg gatgttttta tctgcgttac attaattttt cgcaatagtt aattattccg
    14881 ttaattatgg taatgatgag gcacaaagag aaaaacctgc cattttcccc tactttcaat
    14941 cctgtgatag gatgtcactg atgatgttaa tcacactgac cttacagaat gggccttatg
    15001 aacgcatttg attcgcaaac cgaagattct tcacctgcaa ttggtcgcaa cttgcgtagc
    15061 cgcccgctgg cgcgtaaaaa actctccgaa atggtggaag aagagctgga acagatgatc
    15121 cgccgtcgtg aatttggcga aggtgaacaa ttaccgtctg aacgcgaact gatggcgttc
    15181 tttaacgtcg ggcgtccttc ggtgcgtgaa gcgctggcag cgttaaaacg caaaggtctg
    15241 gtgcaaataa acaacggcga acgcgctcgc gtctcgcgtc cttctgcgga cactatcatc
    15301 ggtgagcttt ccggcatggc gaaagatttc ctttctcatc ccggtgggat tgcccatttc
    15361 gaacaattac gtctgttctt tgaatccagt ctggtgcgct atgcggctga acatgccacc
    15421 gatgagcaaa tcgatttgct ggcaaaagca ctggaaatca acagtcagtc gctggataac
    15481 aacgcggcat tcattcgttc agacgttgat ttccaccgcg tgctggcgga gatccccggt
    15541 aacccaatct tcatggcgat ccacgttgcc ctgctcgact ggcttattgc cgcacgccca
    15601 acggttaccg atcaggcact gcacgaacat aacaacgtta gttatcaaca gcatattgcg
    15661 atcgttgatg cgatccgccg tcatgatcct gacgaagccg atcgtgcgtt gcaatcgcat
    15721 ctcaacagcg tctctgctac ctggcacgct ttcggtcaga ccaccaacaa aaagaaataa
    15781 tgccacttta gtgaagcaga tcgcattata agctttctgt atggggtgtt gcttaattga
    15841 tctggtataa caggtataaa ggtatatcgt ttatcagaca agcatcactt cagaggtatt
    15901 tatggcaacg aatttacgtg gcgtaatggc tgcactcctg actccttttg accaacaaca
    15961 agcactggat aaagcgagtc tgcgtcgcct ggttcagttc aatattcagc agggcatcga
    16021 cggtttatac gtgggtggtt cgaccggcga ggcctttgta caaagccttt ccgagcgtga
    16081 acaggtactg gaaatcgtcg ccgaagaggc gaaaggtaag attaaactca tcgcccacgt
    16141 cggttgcgtc agcaccgccg aaagccaaca acttgcggca tcggctaaac gttatggctt
    16201 cgatgccgtc tccgccgtca cgccgttcta ctatcctttc agctttgaag aacactgcga
    16261 tcactatcgg gcaattattg attcggcgga tggtttgccg atggtggtgt acaacattcc
    16321 agccctgagt ggggtaaaac tgaccctgga tcagatcaac acacttgtta cattgcctgg
    16381 cgtaggtgcg ctgaaacaga cctctggcga tctctatcag atggagcaga tccgtcgtga
    16441 acatcctgat cttgtgctct ataacggtta cgacgaaatc ttcgcctctg gtctgctggc
    16501 gggcgctgat ggtggtatcg gtagtaccta caacatcatg ggctggcgct atcaggggat
    16561 cgttaaggcg ctgaaagaag gcgatatcca gaccgcgcag aaactgcaaa ctgaatgcaa
    16621 taaagtcatt gatttactga tcaaaacggg cgtattccgc ggcctgaaaa ctgtcctcca
    16681 ttatatggat gtcgtttctg tgccgctgtg ccgcaaaccg tttggaccgg tagatgaaaa
    16741 atatctgcca gaactgaagg cgctggccca gcagttgatg caagagcgcg ggtgagttgt
    16801 ttcccctcgc tcgcccctac cgggtgaggg gaaataaacg catctgtacc ctacaatttt
    16861 tataccaaag cgtgtgggca tcgcccaccg cgggagactc acaatgagta ctacaaccca
    16921 gaatatcccg tggtatcgcc atctcaaccg tgcacaatgg cgcgcatttt ccgctgcctg
    16981 gttgggatat ctgcttgacg gttttgattt cgttttaatc gccctggtac tcaccgaagt
    17041 acagggtgaa ttcgggctga cgacggtgca ggcggcaagt ctgatctccg cagcctttat
    17101 ctctcgctgg ttcggcggcc tgatgctcgg cgctatgggt gaccgctacg ggcgtcgtct
    17161 ggcaatggtc accagcatcg ttctcttctc ggccgggacg ctggcctgcg gctttgcgcc
    17221 aggctacatc accatgttta tcgcgcgtct ggtcatcggc atggggatgg cgggtgaata
    17281 cggttccagc gccacctatg tcattgaaag ctggccaaaa catctgcgta acaaagccag
    17341 tggttttctg atttcaggct tctctgtggg ggccgtcgtt gccgctcagg tctatagcct
    17401 ggtggttccg gtctggggct ggcgtgcgct gttctttatc ggcattttgc ctatcatctt
    17461 tgctctctgg ctgcgtaaaa acatcccgga agcggaagac tggaaagaga agcatgcagg
    17521 taaagcacca gtacgcacga tggtagatat tctctaccgt ggtgaacatc gcattgccaa
    17581 tatcgtaatg acactggcgg cggctactgc gctgtggttt tgcttcgccg gtaacctgca
    17641 aaatgccgcg atcgtcgctg ttcttgggct gttatgcgcc gcaatcttta tcagctttat
    17701 ggtgcaaagt acgggtaaac gctggccaac gggcgtaatg ctgatggtgg tcgtgttgtt
    17761 tgctttcctc tactcatggc cgattcaggc gctgctgcca acgtatctga aaaccgatct
    17821 ggcttataac ccgcatactg tcgccaatgt gctgttcttt agcggctttg gggctgcggt
    17881 ggggtgctgt gtcagcggct tcctcggtga ctggctggga acccgcaaag cctacgtttg
    17941 tagcctgttg gcctcgcagc tgttgattat tccggtattt gcgattggcg gcgcaaacgt
    18001 ctgggtgctc ggtctgttac tgttcttcca gcaaatgctt ggacaaggga tcgccgggat
    18061 cttaccgaaa ctgattggcg gttatttcga taccgaccag cgtgcagcgg gcctgggctt
    18121 tacctacaac gttggcgcat tgggcggcgc actggcccca atcatcggcg cgttgatcgc
    18181 tcaacgtctg gatctgggta ctgcgctggc atcgctatcg ttcagtctga catttgtggt
    18241 gatcctgctg attggtctgg atatgccttc ccgcgttcag cgttggctgc gcccggaagc
    18301 attgcgtact catgacgcta tcgacggcaa accatttagc ggtgccgtgc catttggcag
    18361 cgccaaaaac gatttagtca aaaccaaaag ttaatcctgt tgcccggtct ctgtaccggg
    18421 cctttcgcta agggaagatg tatgtcgtta cttgcacaac tggatcaaaa aatcgctgct
    18481 aacggtggcc tgattgtctc ctgccagccg gttccggaca gcccgctcga taaacccgaa
    18541 atcgtcgctg ccatggcatt agcggcagaa caggcgggcg cggttgccat tcgcattgaa
    18601 ggtgtggcaa atctgcaagc cacgcgtgcg gtggtgagcg tgccgattat tggaattgtg
    18661 aaacgcgatc tggaggattc tccggtacgc atcacggcct atattgaaga tgttgatgcg
    18721 ctggcgcagg cgggcgcgga cattatcgcc attgacggca ccgaccgccc gcgtccggtg
    18781 cctgttgaaa cgctgctggc acgtattcac catcacggtt tactggcgat gaccgactgc
    18841 tcaacgccgg aagacggcct ggcatgccaa aagctgggag ccgaaattat tggcactacg
    18901 ctttctggct ataccacgcc tgaaacgcca gaagagccgg atctggcgct ggtgaaaacg
    18961 ttgagcgacg ccggatgtcg ggtgattgcc gaagggcgtt acaacacgcc tgctcaggcg
    19021 gcagatgcga tgcgccacgg cgcgtgggcg gtgacggtcg gttctgccat tacgcgtctt
    19081 gaacacattt gtcagtggta caacacagcg atgaaaaagg cggtgctatg accacgttgg
    19141 cgattgatat cggcggtact aaacttgccg ccgcgctgat tggcgctgac gggcagatcc
    19201 gcgatcgccg agaacttccg acaccagcca gccagactcc gcaggcgttg cgtgatgcct
    19261 tagccgcgtt agtcgccccg ttgcaagctc atgcgcagca ggttgccatc gcttcaaccg
    19321 gaattattcg cgacggcagc ttgctggcgc tgaatccgca taatcttggt ggattgctac
    19381 actttccgtt agtcaaaacg ctggaacaac tttccaattt gccgaccatt gccattaacg
    19441 acgcgcaggc cgcagcatgg gcagagtatc aggcgctgga aggcgatata accgatatgg
    19501 tgtttatcac cgtttccact ggcgtcggcg gcggtgtagt gagcggcggt aaactgctta
    19561 ccggccctgg cggtctggcg gggcacatcg ggcatacgct tgccgatcca cacggcccag
    19621 tctgcggctg tggacgcaca ggttgcgtgg aagcgattgc ttctggtcgc ggtattgcag
    19681 cggcagcgca aggtgaactc atgggcgcgg atgcgagaac tattttcaca cgcgccgggc
    19741 agggcgacga gcaggcgcag caactgattc accgctccgc acatgtgctg gcaaggctga
    19801 tcgctgatat taaagccaca actgattgcc agtgcgtggt ggtcggtggc agcgttggtc
    19861 tggcagaagg gtatctggcg ttggtggaaa cgtatctggc gcaggagcca gcggcatttc
    19921 atgttgattt actggcggcg cactaccgcc acgatgcagg tttacttggg gctgcgctgt
    19981 tggcccaggg agaaaaatta tgatgatggg tgaagtacag tcattaccgt ctgctgggtt
    20041 acatcctgcg ttacaggacg cgttaacgct ggcattagct gccagaccgc aagaaaaagc
    20101 gcctggtcgt tacgaattac agggcgacaa tatctttatg aatgtcatga cgtttaacac
    20161 tcaatcgccc gtcgagaaaa aagcggaatt gcacgagcaa tacattgata tccagctgtt
    20221 attaaacggt gaggaacgga ttctgtttgg catggcaggc actgcgcgcc agtgtgaaga
    20281 gttccaccat gaggatgatt atcagctttg cagcaccatt gataacgagc aagccatcat
    20341 cttaaaaccg ggaatgttcg ccgtgtttat gccaagtgaa ccgcataaac caggatgcgt
    20401 tgtcggcgag ccgggtgaga ttaaaaaggt ggtggtgaag gttaaggctg atttaatggc
    20461 ttaaatttat tagatcaggt gtgacctgac ccatctttgg cacagagcca aacctgaact
    20521 gacggaccgc tctgtgccat tacctgacgt tgtattcttt tatgttaaca cccttgatac
    20581 tgtatatacc tgtccgctta attcgcttgc cctttttatt cgtaatagtt gctgtaagtg
    20641 tactgcagcg aatggaatga cgttcatcaa tcaccagccg aattgcttct gaagcatctt
    20701 ccacctctag aaggtcgtga gtcttattta agccgaatgg aacaagatgg aaggcagttt
    20761 ttcctgatga ttttctggtt atttttactg gatacaacac atcaccactt ttatggatta
    20821 attcaagctg ttcaatgtaa atcatggcaa ctttccttcg tttatttgca tgaatataca
    20881 aaccgaaaat aggagatgca atacaaaagc tgtggaaata tcaaacagcc tctcaatttg
    20941 gcagacagcg tctgccaaat ctttgccgat gggaattctg cagatggtgt ctgcggaatt
    21001 cccaatggtg ttaattcgcc agacactgtc tggcgaattt caacatgttg aaaaaagagg
    21061 taaaaaagcc tcatttggcg taagtttttt gcagagaacc cacgcttata ccttagactt
    21121 aaatcctttg atagtcgctt gattagctgc gcaccatacg cagcccttgc ctcgccacct
    21181 tgttcaaatt cgacaattcg tcggccaatt tcccagtatg tcgcggtcat cagcgcgtta
    21241 acgctacgta ccgtttccgt ccgggcgcta tcgaccaggt gaataatacc gtcgtggatt
    21301 tgctggtagc ctgttgaggt tctctctgag agcgattcca ttatct
//
LOCUS       DADLTD010000054        21074 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLTD010000054 DADLTD010000000
VERSION     DADLTD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.054"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..591)
                     /gene="wcaD"
                     /locus_tag="JN701_001613"
     CDS             complement(<1..591)
                     /gene="wcaD"
                     /locus_tag="JN701_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY7364101.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIK"
     gene            complement(566..1783)
                     /gene="wcaC"
                     /locus_tag="JN701_001614"
     CDS             complement(566..1783)
                     /gene="wcaC"
                     /locus_tag="JN701_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY7364102.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSYQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1780..2268)
                     /gene="wcaB"
                     /locus_tag="JN701_001615"
     CDS             complement(1780..2268)
                     /gene="wcaB"
                     /locus_tag="JN701_001615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY7364103.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2271..3110)
                     /gene="wcaA"
                     /locus_tag="JN701_001616"
     CDS             complement(2271..3110)
                     /gene="wcaA"
                     /locus_tag="JN701_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAY7364104.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGH"
     gene            complement(3288..5450)
                     /gene="wzc"
                     /locus_tag="JN701_001617"
                     /pseudo
     CDS             complement(3288..5450)
                     /gene="wzc"
                     /locus_tag="JN701_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
     gene            complement(5453..5896)
                     /gene="wzb"
                     /locus_tag="JN701_001618"
     CDS             complement(5453..5896)
                     /gene="wzb"
                     /locus_tag="JN701_001618"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY7364105.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSWETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5902..7041)
                     /locus_tag="JN701_001619"
     CDS             complement(5902..7041)
                     /locus_tag="JN701_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7364106.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKTYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQVLMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7700..9283
                     /locus_tag="JN701_001620"
     CDS             7700..9283
                     /locus_tag="JN701_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7364107.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(9557..11410)
                     /gene="asmA"
                     /locus_tag="JN701_001621"
     CDS             complement(9557..11410)
                     /gene="asmA"
                     /locus_tag="JN701_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY7364108.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11432..12013)
                     /gene="dcd"
                     /locus_tag="JN701_001622"
     CDS             complement(11432..12013)
                     /gene="dcd"
                     /locus_tag="JN701_001622"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7364109.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12105..12746)
                     /gene="udk"
                     /locus_tag="JN701_001623"
     CDS             complement(12105..12746)
                     /gene="udk"
                     /locus_tag="JN701_001623"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY7364110.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13064..16361
                     /locus_tag="JN701_001624"
                     /pseudo
     CDS             13064..16361
                     /locus_tag="JN701_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(16400..17257)
                     /gene="alkA"
                     /locus_tag="JN701_001625"
     CDS             complement(16400..17257)
                     /gene="alkA"
                     /locus_tag="JN701_001625"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY7364111.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            17391..18743
                     /gene="yegD"
                     /locus_tag="JN701_001626"
     CDS             17391..18743
                     /gene="yegD"
                     /locus_tag="JN701_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7364112.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(18755..20667)
                     /gene="yegI"
                     /locus_tag="JN701_001627"
                     /pseudo
     CDS             complement(18755..20667)
                     /gene="yegI"
                     /locus_tag="JN701_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(20664..>21074)
                     /gene="pphC"
                     /locus_tag="JN701_001628"
     CDS             complement(20664..>21074)
                     /gene="pphC"
                     /locus_tag="JN701_001628"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HAY7364113.1"
                     /translation="ISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFIT
                     DEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQEQ
                     LDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
BASE COUNT         4937 a         5559 c         5405 g         5173 t
ORIGIN      
        1 tttgatacca aactgtttga tgctgagcca aattgagatt aatgccagag cgaaaaatgc
       61 cggttcgaaa taaagtgctg tcgtgcgctt gccgccgaat ttaatgaaat tcagcacata
      121 gctgttgctg taaatcagat atttcgaaat cgactccata atactgctgc caccggtgag
      181 gataatttgc gccatctcca ctgccgccag cgccaccacc agccctacca ccagataaaa
      241 gaaacgtaat atcttgcgat ggttgtgtgg cgaaattgtt ttaaaacgaa tactccacac
      301 catgccaata atgatcacaa tatagacaaa cagcatggtt gaagtgacgt atttactggc
      361 atccagcgac tgaccaaaca ggtagttaaa cgccgtgagt cccgcaccaa tccctaaggc
      421 aatcatcaat tttttaacgc tgatgcgttc taaaaacagc agtaacaaga caggtaaaaa
      481 agtgacgata gtgatgggga aactttcacc aagctgggcg attttgacgt taaccagcag
      541 gtagatcagc ggcagcagca ggtagctaca gattctgata gaagttgaca tactcctcca
      601 gcatctgttg tccactgtag gcggcgcggc tgcgctggct gaacccggcc aacgtggtac
      661 caaatatcgc ctgcgcgatt tccggtttgc ttaattgcac tagttgcagc acctcttctt
      721 cgctgacggt tttaccgccg gatttttgca acacttcccg cgccgcgtcg ctatgggtgg
      781 caatcaccgg cacgccaatc gatagcgcct cacacaaaat cagcgggtag ttatcgacgc
      841 gagaactgaa caccagcgca tccatctgat tgagcgcgct catcaacttg cgcttgtcag
      901 tttcaaagcc gtgattaacc acgttgccag cggtgaacgg cgagaactta ccaaaggtat
      961 gcagttcgat tttgtcgccc agcgccatca tctcacgcac cagttgctgg ttagtcttgc
     1021 cgtcgtaacg caagtcatgc gccaccaccg cgattttcgg cttgccctgg gtttcgcgca
     1081 ccggaggcaa gtccgccaga atcgcttcgg ttgccatatc aataccatta ttgataatcc
     1141 ggcaacgccc tggaccgtac aggctattga aagcgtcagc cacatgctgg ctgggggaaa
     1201 taaactgaca gcccagcgcc agcatctcac ggaataactg gcgtttgccc gccaccagct
     1261 ggtgtgcgcg atcaatcttc accggcggat agttattcaa ggtcgggcat ttctggcagc
     1321 ccgttttcca gccttcgcaa ccgtcggtaa aggcgcagcg tccggtaaca ctccagtggt
     1381 cgtgcagcgt ccagaccaga gtgacgtccg gtttatggtt tttcactttt tcgcaaaagc
     1441 gcaccacgct cttaagattt agccagtagc tgtgcagcac atgaaaatgc aggaccaccg
     1501 gacccggtgt gcgagtaatg gtgcgatata actcattgaa attgccaaac agatcgcgat
     1561 taaacagacg aaacagggca atattcgcca tcgcggtcat ccgcggcgta tgtttgatga
     1621 cctgcggata gttctgatag ctgacgctct ctttgccgcc tttgccgtaa ccgtagacaa
     1681 aatgtgacgc cagcccctgt tgcagcgcac gctggtggag atctaacgcc acacctgctg
     1741 ccccgccttc cgccagtcgc acattaaatt gcagaatatt catttaatta ccttcactcg
     1801 cgctttttct cctaccacca gcgcgttgtc cgggacagag tcgagcacca cgctgcccgc
     1861 acccacggtg acgttgttac caagcgtgat atcaccaaga ataatgacgt tggcaccgag
     1921 ttcgacgccg ttgccaatgt gtggacatgc catgttatcg gcaccacgat tgccgatagt
     1981 gacgccgtgg cgaatggtga aatcatcccc cgctaccacg tttttattga tcactacggc
     2041 gtaaccgtga tggatagtaa agcggcggcc aatggtcgcg gcggcctgga tttcataacc
     2101 gaaaaagcat tcggtgataa tgcgatacag caccagcagc ggggccgccc acagattgtt
     2161 gaggacgttc tttttgcgcc acaccgaaca aaaatgagca acacgatagg caagaaccat
     2221 gcagcacggg cgtaaactcc agctgttggc acgcagatct tccagcatct ttagtgcccc
     2281 cgaataccat cagccagacg tttgccgtta cgcaccgaca gcagcgtcag caaagtgcgc
     2341 caggtcatac gtttattgcg gatctggtag agggtaaaca gctgatattt tttgcttgca
     2401 cggtcaaatt tatctttgtg ctttcggtaa aagtggaagt acccggagaa ttttttcggt
     2461 gacgaggtga tctgcatttc accgtgattg atatgcagga tctgcgtcgc ttcttccact
     2521 ttccacggtt cgccgtactc caccaccatc cgcaggaaga tgtcgtaatc ctgtgctgct
     2581 ttcagttcgg tatcgaacaa acactctttg aaacgccatg cccaagtaaa gacctgattg
     2641 ccaatgatat tgcgtttata gaacaggcgg cgtgaatacg gcgatttggg atatagcggc
     2701 aaacttgctg gttgggaata gacttcgccc tggcaaacgt agtcgttagc gtacaaaaag
     2761 gcgtgagtga ccagttgctg tttatgggcg aggaagacgc tcagacggtt gggtgtccat
     2821 tcgtcatcgt catcaatccc ggtgatgtat tccccttgcg ccagcataat cgcctggtta
     2881 cgtaccgcgc acgccccgct gttaatatcg ttgtggatgt aagtgatacg cggatcgttg
     2941 agggcggtga cgtactgttg cagttgttcc caggaggtgg agcaatcatc cacgatgatc
     3001 atctcccagt tgctgtagtc ctggcgcagg accgatttta tcgcccgaat cgccagttgt
     3061 tggcggttcc aggtcggcat atagattgag atcagcggat tgtctttcat aggttgttct
     3121 cccggagtgt cgagatgttc tgccggttta taagcttgtt tacgggttct gttgccggat
     3181 gcggcgtgag caccttatcc ggcctacgga gctggtttgc ttgccggatg cggcgtgaag
     3241 gtaggtcgga taagatgcgt cagcatcgca tccgacagcg gatgtcgtta tttcgcatcc
     3301 gacttatatt cgtattcgta atagccataa tcctgatacg cgctggcgcg gcggaagatg
     3361 gagttcagaa tcaccccttt caccggaata ccgttttgct caaagcggct cagactggtt
     3421 tccacttctt tcaaggtgtt gaccgcataa cgcgccacca ttaacgtggt tccgacatga
     3481 cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg gcgtatcaat caacaccagg
     3541 tcatagtttt tactcgccca gttcaccagt tcggcaaagc gttcgctcat caacagttca
     3601 gaaggatttg gcggcacctg accgcgcggg atcaggtcaa atttggcaat agaggtcggt
     3661 ttagcagcgg tagtaatatc gccctgacca atcagaattt ccgacaggcc attaacgtta
     3721 ttagtgcaca gcagctcgtg ggtgtagcct ttgcgcatat cgcagtcgat caacaacacc
     3781 cgtttattgg tctggctgat caccgccgcc aggttggcgc agacaaaggt tttaccgatt
     3841 gacgggctaa ccccggtcat catcaacaca ttgttctgtg cctgcatcat cgcgaagtgc
     3901 aaactggtac gcagactgcg gatagcttca atcgccagat cggttggatt ccccatcgcc
     3961 agtagctggc tctgtttata gcgtttaatc cctttgatgg ttttgacgct atcgcgcgct
     4021 ttctgccatt ccgacagcgg gatgctggca tagacgctga taccgtgttc ttccagcacc
     4081 tgcgggcttt cgatgccgcg attaaacaac gagcgcagca gcacacccac gatagaaagc
     4141 atcaggccaa ggataatcgc gccgaggata atcagccctt tcttcggttt tagcacgcca
     4201 ggctgggtga ttgccgggtc aacaatgcgc acatcgccga cggtgctggc ttcggtgatt
     4261 ttcagctcct gctctttatt cagcagttgc atatagacct gctgaccaga ctcgacatcg
     4321 cgggtcaggc gcacaatctc ctgctgggtt ttcggcatcg ccgttacgcg accgttaagt
     4381 ttggctttct cttcttccag cgcctgacgt ttctccagca gcgtgcggta cgccgggtga
     4441 actttggtgt acagcttgga gatttccgcc tctttaaagg tcagttcgtt caactgcgcg
     4501 tcgatgttca ccatcgaatc gagcaccgct ttcgcttcca gcggcagatc aacagaatct
     4561 ttatcctgac ggaaggcatt cagtttgttt tcggcaacat cgaggcggct acgtacttcc
     4621 ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt ccgccgattt gcgctcaata
     4681 ttttgttcct gatagttacg ggcgatgctg ttaagaatgt cgcggatctg ttcgcgatct
     4741 tcaccggtat aagtcaggct cagtacgcct gcgtctttgc cgttctccgt taccatcagg
     4801 ctgttttgca gttggttgat catccccaac gtggagtatt tggtgacggt aaactcactg
     4861 cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc cttctttttt cagcatctgg
     4921 cccgcttgcc cacgggcgct aaagccgcca tcactgctca gggtgtagtt tttattgtcc
     4981 agcacattaa gcgtaaacac ctagtccgcc atctctttcg ggcggttaaa ggtagtcact
     5041 ttcaccgtct cgttctggcg tcccatcagt cgatcccagc ccgccccgaa aatcgggaac
     5101 gtgtttttgc tcactgcaat atcgaggtcg agatcgtcca ccgttttacc aagcaccaga
     5161 cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg gtttgttggc taatgccgaa
     5221 ccaatgtcct gcactaacga attgccgctg ttttgctcga tttgtaccag tgcgtcggca
     5281 ctataaatcg gcgtggcgaa gaaggtgtaa accacggcac agaggacaaa tacagcggtg
     5341 atgccaatca cccaccagcg cgcttcaatg acggtgccga ccaggcgacc aatatcgatt
     5401 tcatcactgc ccgttaccgg agcggcatgt tgttttactt tttctgtcat tcttatacct
     5461 gctctgcgtt caatgcctgc gcccactggc gggcagaccg ttcaagtaat gtgtacaccg
     5521 ctgcaaacgt ttcccagctt ttgcgatacg gatcggggat ttcacattcg ttatcccagt
     5581 gaccaaacag catcactttg ccgcgcatct caggtgccat ctcgcataag cgttcgatat
     5641 ggcgcttttc catggtcaaa atcaggtcat agttgcgaca caggcggcgg ctgatttgtc
     5701 gtgcacagtg gccttccaga gacagttgat gttctgcggc gacgctgata gcggtaggat
     5761 cagcgccctt accgaccagc gcgccgagtc cagcggactc cactttcagt tccgggtgat
     5821 aacgttgcag taagcgttcc gccgtcgggg aacggcaaat attgccgaca cagacaacta
     5881 atatgttgtt aaacatgacg attaccagtt atgaatgtcg ctggctgtat ccgtcatgta
     5941 acggacaccg ctaatagttg gcagcaactg attgatcaga cggttccagc gggaaaccgg
     6001 ggcggtggtg acatacacca cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag
     6061 cgacgtggca tcggacatat ccagctggta gatattggca atcttgccgt tacgcccgcc
     6121 ctcgcctttc agcggacgaa tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat
     6181 accttcagca ttacccaggg cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt
     6241 gctctgtttc ttcacttcac ccatcacaaa tactttcaga tcatcattac gcggcacgta
     6301 gagaatatcg ccgggataaa gcagtcggtt ctgattgagg tcgccgtttt gcatcagcac
     6361 ctgcaaagaa atgcgttctt cacgaccatt gtgtgttagc accacgttgc gccagtcagc
     6421 ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga atggtcagtg gtacgttggt
     6481 gatcgcctgt tgaccggatt tattcacctg acctgagata taggtctttt gtgagcggaa
     6541 ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac gtcgctaagc gcccggtaat
     6601 atcactgcgg atttcagcga gcgttttccc gactacgtga accttgccga tatacgggta
     6661 aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg ctggagctgc ggtactgacc
     6721 agctggcgtg gtgagttccg ggtgatccca gacggtgaca ttaagaacgt cccccggccc
     6781 gacgcgatac tggtaattcg cgatctcgct ttccagcgtc atattggggc gcgctacatt
     6841 cgggcgtggg cgtaattggt caatcaggcg cggggtcagc ggataaacat tcaccatttt
     6901 gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct ttgcccatcg tcgacatatt
     6961 gctgcccgga agtactgtgc aaccgcttat caaggttact gacaccaata atggcatcaa
     7021 tttcattttg gatttcatca ttgtttattt atcactttgg cagagtaatt atcctgtgca
     7081 ctattaatag caatgtcgcc atgcacattt accttgcagt taattgaata caaatttaac
     7141 tagcatcagt cctaaaaaaa ttgatttcat ccgcaggcta ttgacagaat aattcagact
     7201 ggtctttcag gcatccagac acgctaccgc ccttggcttt ttagccacca atacactgat
     7261 ttagcttaat tttttaaacc ctctctgcat acaatcgttg atgagaaagg gttattgcgg
     7321 aaattaactt ccgaatataa ggtgacatta tggtaattga atattggctc tccaataatg
     7381 caggaggaag tgttacagct aacggaatag caggcaaggt aacaattcgg caattggcta
     7441 tttttaagaa ttatattaag tgtcattcaa tatggttttt aggagtttct ttaggttgac
     7501 aatatttaat attgcgcctc cgcatgcgat atttcttaaa taatgtttta ttattaccac
     7561 ttttaattca gggataatgt gaggttatta ccctcaaata atatgagata aatagtgctg
     7621 caacattgca tttttgcccc gatttatcca tgatcgaatt gtgacatttg tcatacaacg
     7681 aataggtttt gtacttacta tggaatggat tgccgatccg tctatctggg ccgggttaat
     7741 cacgctgatt gtgatcgaac tggtcctcgg cattgataac ctggtcttta ttgccatcct
     7801 cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc tgttgctggc
     7861 gatgttaatg cgcctgttac tgctggcgtc aatctcctgg ctggtcaccc tgacgcaacc
     7921 gctgttcagc ttccgctcgt ttacctttag cgcccgcgac ttaatcatgc tgtttggtgg
     7981 tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa cggctggaag ggaaagacag
     8041 caataatccc acacaacgca aaggtgcgaa gttctggggg gtggtgacac aaattgtggt
     8101 actggacgcc atcttctcac tcgactcagt gattaccgcc gtcgggatgg tcgaccattt
     8161 actggtcatg atggccgccg tggttatcgc tatcagcctg atgttgatgg ccagcaagcc
     8221 gttaacgcaa tttgttaaca gtcacccgac catcgttatt ctctgcttaa gcttcctgtt
     8281 gatgattggc tttagcctgg tggcagaagg tttcggcttc gtcattccga aaggctacct
     8341 gtacgctgcc attggtttct cggtaatgat cgaggcgctc aatcagttgg ctatctttaa
     8401 ccggcgacgt tttctttccg ctaaccagac gctacgccag cggactaccg aagcggtaat
     8461 gcgcctgctt agcgggcaaa aagaagatgc ggaactggac gccgaaaccg cgtccatgct
     8521 ggtggatcat ggtaaccagc agatctttaa tccgcaggaa cggcggatga ttgagcgggt
     8581 acttaatctt aaccagcgta ctgtcagcag cattatgacg tcgcgccacg atattgagca
     8641 tatcgatctc aacgcgccgg aagacgagat ccgccagttg ctggagcgaa atcagcatac
     8701 gcggctggtg gttaccgacg gtgatgacgc agaagatttg ctcggtgttg ttcacgttat
     8761 cgacctgcta caacagtcac tgcgtggcga accgctcaac ctgcgggtgt tgattcgcca
     8821 gccgctagtg ttcccggaaa ccttgccgtt gttacctgcc ctggagcagt tccgtaatgc
     8881 ccgcacacac tttgcttttg tggtggatga gtttggctcg gtggaaggga ttgtgacatt
     8941 aagtgacgtc actgaaacca ttgccggtaa cttaccgaac gaagtggaag agatcgacgc
     9001 ccgccatgat attcagaaga atgcggacgg ttcctggacg gcgaatggtc atatgccgct
     9061 ggaagatctg gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat atcacaccat
     9121 tgccgggctg ttgatggaat atttacagcg tattccaaaa cctggcgaag aagttcaggt
     9181 gggggattat ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga aggtgcagat
     9241 tattccgctg cataaggatg gcgagttgga gtacgaggtg tgatggtatc ggtcatttta
     9301 cccaatagcc gaaaggaata agatgaggga accgcgccag cggcgattta ccgccgggag
     9361 cccgggttgc cagggtggtg gcgattgagc ccccctggca cgttcaccgg cttgagcgtt
     9421 ccatagtagc gaggaacatc aagtgaacgg aacagccttc gatcttacat attcagaata
     9481 taatgatgcc gccacgaacg cctgatgcga cgctttacgc gtcttatcag gcctacagga
     9541 caggtcacca ggtaacctac atcttctcca gcaacttctt cacatctttg ccattgcggg
     9601 aatctttatt ccgctcggcc cagtcattca gacgacgttt cgcttcgtcc tgtagatgtt
     9661 tgcgcagtaa ctgatccact tgcagactgt aattgagttg ctgccagttg ccataaaccc
     9721 gcagcggtac tggcgtttct ttaaggaaat caatcagttt gctttccccg ttccagccac
     9781 ccacgacccg aatatcaaac tgggtgtcgc aggtttgctc cgccagattc aacgtaccag
     9841 ccccggaaag cgccagcaca ggcgattgac cttgcatgtc gtttaacgtc acgacgccat
     9901 ccttcaacgt caaatcggtg gtaaagcggt caagacgcgt tacgttatcg aagttttcag
     9961 cggccttcac atcaccacca ttacgttcta ccgcttgctg aatcatctgc tggaagttca
    10021 tcccttccat gcgcgtgtcg gtcatttcga catgtgcctg tccttgccag ttgtggcgga
    10081 atgcgtcggc atctatgtca gcaccggaga aatcaccagc cagtgacatc tttccggtca
    10141 acgaaatcgg atagttaaac gccttcagaa tggtgccaat ctcaacgttt tccagccgtg
    10201 gctggaagtt tatccgcgga tttattgatg tcgcgtccag cgtgcccggc agtgaaacct
    10261 gtccaccgtt aagcttgccc tgcagttgag taatttccag caaacccgac ttgttggtca
    10321 tttgcgtggc aacatctgta aaattcattc cgcgccagcg cacgttactg gcctgcaaca
    10381 aaatatcagc cgtaaagcct tgcagtccct gatatgccgg ttcatcaata cgcgaagaaa
    10441 tgaccgggcg cggcaacgtt gattggctct gcccctgctg cgcggcaccg ttttcaccat
    10501 tcgctgtttc attaagcggg atgaggttgt caagattcag ttgcgggaat tgcagtctca
    10561 gctgccattc cggtttctct gtcagcgtga cctgtgcttg cccgctcagc gtactgtcat
    10621 tggcggtcaa actaatttgg ttaaatgaaa ggcgtttatg tgactcctgc cactgggcct
    10681 ggaaactccc ctgcccatga ataccttgtt ttggtaaatc tgcaccctgc aactgccagt
    10741 taatttgttc aatagccgcc gttaaatcat gcggataatc agaagcatct accgtaccgt
    10801 taagggatat cgtgagatcg cgctgatcgc gattaacccg cccggagaac tcaaatgaac
    10861 cacgatgttg gggatcttgt tccatttgca ggcgaatatt gcggattgtc acctgctcgt
    10921 catcttcatg ctggaacacc agcacgctgt ccgccacctt aagactggat atatcaaacg
    10981 accatccgcg atcgtctgac agatccggca aggtattgtc gcgcggtgca accggagcgt
    11041 cttcactgcg caccacttcc gtctgcggcg tcagttggat cactgcccct tttagcatca
    11101 cctgcttaac gctcagttga tgactcagta gtggtaaaag cgccacgtcc agacgcatgt
    11161 tgtcggcgcg aaccagtggc tggctcgcgc cctgggcggt gagagacatt cgcccggaga
    11221 ggatactaag ctgcggccag acgtgccaac gcagtggccc gtcgagctgc aattgataac
    11281 cgctacgtgc agcaacttgc ttgaccatat agttgcggaa atcattcgga ttcaccagca
    11341 acactaacgc agataacccg gccaccagca cgaccaggag tatcatcagc gtcgtcagaa
    11401 atcgtctcat ggtatcctca atgggcctga attagtcttt atcgattcgg ctggctaccg
    11461 cgccctgctg gttgcgatat ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc
    11521 cggaaagcgg ctcaaagctc agcgcaccaa ttaacatgcc cggacgcagc gccagcggca
    11581 gcttaccgga gttgtagaac tccagcacaa tgcaaccaga ccagcccgga tcgatacggt
    11641 gcgcggtgac gtgcaccatc agccccagac gcgccaaaga ggaacgcccg tccagccagc
    11701 ccaccagatc ggctggcagc gtcaccgact ccagcgtcac cgccagcgcc agttctcctg
    11761 ggtgaagata gaacgcctcg ccctcgtcga gaacgatctc atcgctcatc acgcggtcaa
    11821 gcgcggcgct cacttcatct ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac
    11881 ggaaggtacg aaatttattg cccaggcgta catccaccgt cgcgccgtta atacgctcca
    11941 ctggcggacg tgggttgatc gacaaacggc cttcatcaag ccaggcttca atatctcggt
    12001 cacacagacg catggcattt tctcctttcg cgcatcactc ccttaacgcc aattacgtca
    12061 agggcatacc aggttatcac tgaacggtac acaatttatc aagcttattc aaagaactga
    12121 cttattttcg ctttcaatat atcgatcgcg atgcggtttt tcccgccgcg cggcacgata
    12181 atgtccgcat attgtttaga aggctcaatg aattgcagga acatcgggcg cacggttttt
    12241 tgatattgcg ccatcacgga atccattgaa cgcccacgct cgttaacgtc acgcttgatg
    12301 cggcgcatca ggcagatatc cagcggggta tcaacgaaaa tggagaagtt aagttcgtca
    12361 cgcaaacgcg catccgtcag caacaaaatg ccttcgagaa tgatgacctt cttcggctca
    12421 accgtcaccg tttctttcat acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt
    12481 gccgagccgc gtttcaacgc ttgtaaatgc tcaagcagca ggttgtgatc catcgcgctg
    12541 ggatggtcgt agttggtctt aacgcgttct tccatcgaca gatggctttg atctttgtaa
    12601 tagcagtctt cgggaattac accgatgtgt tcatcaccga cttgctcacg taattcacga
    12661 taaagggtac tggcaataag actcttgccg gaagccgatg cgccagcgat accgataatg
    12721 acgcactgat gagactgatc agtcatatat ttagcgacct gattaacctg gatgttagga
    12781 agggggcgac gaagcgccaa acgcggcaat tatagggatt tcatccgcct gataccagtc
    12841 gaatagcgtt gccgcgcgct cagagttaat tgttgacaaa gaattcccgg tggcaaatta
    12901 cgttgatcag ttttatgtaa ggtaaaaaat gttatacgca gttgcgcaaa ttatccgcct
    12961 ttacgtcact ttatgagcaa ttcacatata aaatgtaaaa cttttgtact agcataaaca
    13021 cagaaacgaa tactggcgac ctggtcttgc ggataaagcg gtaatgagca aacaatcaca
    13081 gcatgtatta attgccctgc cccacccact gcttcacctg gtcagtttag gtttagtctc
    13141 gtttatcttt acccttttct cgcttgagct ttcgcagttt ggcacccaac tcgccccact
    13201 gtggttcccg acgtccatca tgatggtggc gttttatcgc catgccgggc gcatgtggcc
    13261 gggaattgcg ctgagctgct cgctgggaaa tatcgccgca tccatcctgc ttttttccac
    13321 cagctcgctg aacatgacct ggacgaccat caatattgtt gaagccgtgg tcggggcagt
    13381 gctgctacgt aaattgctgc cgtggtataa ccccttgcaa aatctggctg actggctgcg
    13441 tctggcattc ggcagcgcca ttgttccgcc tctgttaggg ggtgttctgg ttgtcctgct
    13501 gacgcccgga gacgatcctc tcagggcatt tttgatatgg gtactgtcag aatccatcgg
    13561 cgctctggta ctggtgccgc tgggattgtt atttaagcca cactatctgc tgcgccatcg
    13621 caacccacgg ttactttttg agtcactgct cacattagcc atcacgctga cgttaagctg
    13681 gctttctatg ttgtacctgc cgtggccttt tactttcatt attgtgctgt tgatgtggag
    13741 cgccgtgcgc ctgccacgaa tggaagcctt tttgatcttc cttaccacgg tgatgatggt
    13801 gtcgctgatg atagccgcgg atccctccct gcttgctacg ccgcgtacgt acctgatgag
    13861 ccatatgccg tggctactgt ttttgctgat cctgctgccc gccaacatca tgaccatggt
    13921 gatgtatgcc tttcgtgcgg aacgcaaaca catttccgaa agcgaaaccc gctttcggaa
    13981 cgcgatggaa tattccgcta tcggtatggc gttagtgggc accgagggac aatggctgca
    14041 aaccaacaaa gcgctctgcc agtttctcgg gtacagtcag gaagagctgc gcggactcac
    14101 ctttcagcaa ctaacctggc cggaggatct caataaagat ctccaacagg ttgaaaagct
    14161 gataagcggt gaaataaaca cctattcaat ggaaaaacgc tactacaacc gtaatggcga
    14221 tgttgtctgg gcgttgcttg ccgtctcact ggtgcgccac acggatggca cgccgctcta
    14281 ttttatcgct cagattgaag acattaacga gctaaaacgc accgaacagg tgaatcagca
    14341 actgatggag cgcatcacgc tggctaacga agcgggcggg attggcatct gggagtggga
    14401 gctgaagccg aatatttttc gagctgtatg aaattcctcc gcatatcaaa ccgaactggc
    14461 aggtgtggta cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa gtgattcgtg
    14521 attcgttgca atcacgctcg ccctttaagc tggaatttcg cattaccgta aaagacggta
    14581 ttcgccatat ccgcgccctc gccaaccggg tactgaataa agaaggcgaa gtcgaacgcc
    14641 tgctcggcat taatatggat atgaccgagg ttaaacagct taacgaggca ttgtttcagg
    14701 aaaaagagcg cctgcacatt acgctggatt ccatcggtga agccgtggtc tgtattgata
    14761 tggcgatgaa aattaccttt atgaatccag tggcggagaa gatgagcggc tggacgcagg
    14821 aagaagcatt aggtgttccg ctcctgacgg tgttgcatat tacttttggc gacaacggac
    14881 cattaatgga gaacatttac agtgccgaca cctcacgttc cgcgattgaa caagatgtgg
    14941 tgttgcactg ccggaacggc ggcagttacg acgtgcatta cagtattacg ccgttaagta
    15001 ctctggacgg cagcaatata ggctcggtac tggtgattca ggacgtcacc gaatcacgca
    15061 aaatgctgcg ccagctgagc tacagcgcct cccatgatgc actgacgcat ctcgccaatc
    15121 gcgccagttt tgagaagcaa ctgcgtatcc tgctgcaaac ggtaaacagt acacatcagc
    15181 gacatgccct ggtgtttatc gatcttgatc gctttaaagc ggtgaatgac agcgccgggc
    15241 atgcggcggg tgacgcttta ctgcgcgaac tggcgtcgtt gatgctgagt atgctgcgct
    15301 ccagcgacgt gctggcgcga ctcggtggtg atgaatttgg tctactgttg ccagactgta
    15361 atatcgaaag tgcgcgtttt atcgctacac gcattatcag tgccgtgaat gattatcact
    15421 ttatatggga aggccgtgtg catcgggtag gtgccagtgc cgggattacc ttgattgatg
    15481 acaacaatca tcaggcggca gaagtgatgt cgcaggctga tatcgcctgt tatgcctcca
    15541 aaaatggtgg acggggccgg gtgacggttt acgaaccaca gcaagctgcc gcacatagcg
    15601 aacgggcggc gatgtcgctt gatgaacagt ggcggatgat taaagagaat cagttgatga
    15661 tgatcgccca cggtgtcgct tcgccgcgga tcccggaagc gcgtaatttg tggctgattt
    15721 cacttaagct ctggagttgc gaaggcgaga ttattgatga acaaaaattt cgtcgtagct
    15781 tcagcgatcc ggcacttagc catgctcttg accgacgggt attccacgaa tttttccagc
    15841 aggccgcaaa agcggttgcc agtaaaggca taagcatcgc cctccccctt tccgttgccg
    15901 gtttgagtag cgccacgctg gtgaatgatc tgcttgagca gctgggaaat agccctctac
    15961 caccacggtt attacatctg attattccgg ctgaagcgat tttagatcac gcagaaagcg
    16021 tgcaaaaact gcggctggtg ggatgtcgga tagtgctcag ccaggtgggc cgcgatctgc
    16081 aaatcttcaa ctcgctgaaa gcgaatatgg cagattacct gctacttgat ggtgagttat
    16141 gcgccaacgt gcagggtaat ttgatggatg agatgctgat tacgattatt caggggcacg
    16201 ctcagcgact cgggatgaaa accatcgccg ggccagtcgt tttaccctta gtgatggata
    16261 cgctttctgg catcggcgtc gatctgattt atggtgatgt gattgccgat gcccaaccgc
    16321 tggatttgct ggtgaatagc agttatttcg cgattaactg agagagcgtt tgcccattgc
    16381 ctgatgcgac gtatcaggcc tacaattcgt ccgttccgtc tggttgccag ccttccgtat
    16441 accagatatg caacagcgca taagaacgcc agggcttcca gcgctcggca taacggcgga
    16501 tttgtgccgg tgtcatcccc ggaaatcgct gtttaatcag ataatcatcc ggcagaaaaa
    16561 catctttcgc ctgccagcca cgcaaagcga aataattcgc cgtccagcgc ccgatacccg
    16621 gaaaagtttg cagcgttttc atcgcctgct ccacatcgcc cggaattgtc attggtaagc
    16681 tgccctccag cgccgcattt gccagatgaa tcagtgcctc tgcccgtttc aacggcatgc
    16741 ctaacgcttt taatgcctgc gggtcggctg ctgccagccg ctgaggcgtg gggaagcaga
    16801 tatactccgg aaaatcatcc agccgttcgc cataaagctg tacaactttg gcagtcaatt
    16861 ttgccgccat cgccacgctc accagttggc ctaaaatcgc ccgcacaccc tgctcaaaag
    16921 catcaataca gccgggtaaa cgcaatccgg gccgcgccgc gcctaacttg cccaacgcac
    16981 cgttaacaat ctgcgggtta cattgcagat caaacaggcg gctcattttc gccagacact
    17041 cagcggcaac gggttctaaa cctgcactta aatttatgtg cagagtatgg cgggctatat
    17101 ccggaatagc agtcaccacg ccgcgatatt cgcccaccgc cagactacgg gcataataat
    17161 cgtcagcgac cgtttccaca ccactcactg cacgggcggc gagaaatccc aacatccacg
    17221 accagtcata cggcggctgc cagttcaggg tatacatcgc atctccttat tcatccgctt
    17281 tcagcataaa cgttattcag acgctacgct ttgctttcat attccggttg tcgcgacggc
    17341 aacatttcgc taaagtcacg ccccttcttc accggcatgg ggattatttc gtgtttattg
    17401 gttttgatta cggtacagca aactgttcag tggcggtcat gcgtgacggt aaaccgcact
    17461 tgctaaaaat ggaaaacgac agcacgctgc tgccttcaat gctttgcgcg ccaacgcgtg
    17521 aagcggtaag cgaatggctg taccgccatc atgacgttcc ggcagacgac gatgaaacgc
    17581 aggcgttgtt acgacgggcg attcgttata accgcgaaga agatatcgat gttacagcga
    17641 aaagcgtgca gttcggtctt tcctcactgg cacagtacat tgatgatcca gaagaagtgt
    17701 ggtttgtgaa atcaccaaaa tcgttcctcg gtgccagcgg cttaaaaccg cagcaggtag
    17761 cgctgtttga ggatctggtc tgcgcaatga tgttgcacat tcgccagcag gcgcaggcgc
    17821 agctgccaga agcgattact caggcggtga ttggtcgccc gatcaacttc caggggctgg
    17881 gcggcgatga agcaaacgcc caggcgcaag ggattctgga acgcgcggcg aagcgtgccg
    17941 gattcaggga cgtggtattc cagtatgagc cggtcgcggc tgggctggat tacgaagcca
    18001 ccttgcagga agaaaaacgg gtgctggtgg tggatatcgg tggtggtacg actgactgtt
    18061 cattgctgct gatgggaccg cagtggcgtt cgcgtctcga tcgtgaagcc agcctgctgg
    18121 gtcacagtgg ttgtcgtatt ggcggtaacg atctggatat cgccctggcg tttaaaaacc
    18181 tgatgccact gctgggtatg ggtggcgaaa ccgaaaaagg catcgccctg ccgatcctgc
    18241 cgtggtggaa tgcggttgcc atcaacgacg tacctgcgca gagtgatttc tacagtagtg
    18301 ccaacggtcg tctgcttaac gatctggtac gcgatgcccg cgaaccggaa aaagtggccc
    18361 tgttacagaa agtctggcgt cagcgtttaa gctatcgcct ggtacgcagc gcagaagaga
    18421 gcaaaatcgc cctttcaaac gctgcggaaa cccgcgcctc actgccgttt atcagcgatg
    18481 aactggctac gctgattagc cagcaagggc tggaaagcgc cctcagccag ccactggcgc
    18541 ggattctgga acaggtgcaa ctggcgctgg ataacgccca ggaaaaaccg gacgttatat
    18601 acctgaccgg cggtagcgcc cgttcgccgc tgattaaaaa ggctctggca gaacagttgc
    18661 cgggcattcc gattgcaggc ggcgatgact ttggctccgt caccgccggg ctggcacgct
    18721 gggcggaagt ggtatttcgt taaaagaaaa tggctcagca gcggcttaaa tctgcctgcg
    18781 cctgagccag tttttctgcc gcctgacgta acggttccat caacggcatg gtccgtgctg
    18841 gagcataaag gcggaatcgc tgcaactctg tcgcgccgac agtcaacgcc gattccagcc
    18901 gatgtcgttt ggcggccatt accgcctgtc tgtctgctgg cgttaccgct tcgttcggcc
    18961 tgaacacaaa acggcggtca cagctcgctt tccagtcgat aaccgcctgg gtcagatggt
    19021 cgccaaaccc tttaacttgc ttaacgccac gacgggtaac atccgctgct gtttcaatac
    19081 caaaagaccg taacgccgct ttacgcgcag ggccaacgcc gggaaaaatc cctccagaaa
    19141 cttctgcttc tgccgttccc ttgcggtgtc ataaagtgct gccagagcgc gtttctcctc
    19201 ttccggtaac ccgagaattt cgtccttcat tttttcgagc atcgcccgtt tggcgataaa
    19261 accttccagc ccgcccaact gttggatctg gctgactaaa tgctcatagt ccattttcgc
    19321 gcgattaaat gcctcacggc gttgctggat ttctgctttg tacgctttgc ttgtcagact
    19381 gccaataatc cagatagccg acagcacagg aaccaaaata atataacgcg gttctgcctg
    19441 aaggccgcaa agcaacagcg ataacgctga cagtgcgatc tcaatcagaa tgatgtattc
    19501 gcgccgtaac aggcctaaag gaagcggcct gccagtcggt tggaaatgat cgggtaatgg
    19561 caattgcaat gctggcggtg ctactgacgc catcaccatc gcccagactt tcgccagcac
    19621 aaaatcaccg ctggtggtaa tgacctcttc accgagatca acaaaataga taacgccttg
    19681 attatccagc gtacaccacg ggcagtcggt caaatgagcg ggataaacat gcattgccga
    19741 aacgggacat ttctttaact gttggcgcag agaatcaagt gccgctaccc acgctttagc
    19801 cgtcggacgc ccggttgcca cgccactttc cgtgaatgcc tgctgaaaca tggcttcaac
    19861 atcactcggt aacatcgata gcggaatcga tcgtggcggc ggttttaaac cacgtcgctg
    19921 attatctgac gcgtaggcat aacggaaatg ggtaatatcc gtctccagcg cattacccgc
    19981 atcagagata agcggcacac cagaataagg atgccggcca ccaaacaaga cgtgaaaaat
    20041 cagcaacgca aggccaaaat tatcgtgatt ttctgtgcgt tcaaagccga caaatgacga
    20101 caaggtttgt agctctggcg gcgtaaaatg cgacacgccg acttcgcata aatgcagtgt
    20161 gccattgacg ttaatctgaa aggagtcgct atcaatcagt accactttgc tgtcgcgacc
    20221 taccataaaa ctgttctggt ttacgtcacc cacgacgtgc ccgtgctcgt gaaccgtagc
    20281 aaaagatgaa gcaatattgc gcgcaacata gagtagaaaa tcccacgcac aatgagggta
    20341 actctggcga cgatgtgccg ggctatagat catatgaatc ggttctttac cagaaacttt
    20401 tggcatcata aaaccgataa cttttccgcc tcgtccaccg tgaagcgttg cctgcggcca
    20461 ggcgacataa ttcaacaact gcgcgtcagc tgtcgcagcc ataaaggcaa gtttgtcctg
    20521 ttttgaggcg ggtggcggcg tgtgataaat cttggcgacg ctatcgacaa actcctcgat
    20581 atcataaacc gcgccttcac cgcctttgcc cagttcacgg cctggcgtaa cgcactcacc
    20641 agtagcagta tataaagtgg gtttcatggt aaccacagcg ccagtgccaa cgttttatca
    20701 tcatccgtac gctcgtttac cgccgggctg gacaaaaact gctttaacaa ttcaggtaat
    20761 aaatcgagtt gttcctgcgt tactgacgcc agtccattga aaaacggggt aaagaaaggc
    20821 acatgaggag aattatccag catatttaac gccagccgct gaataccatc cgtaaatgct
    20881 gcaactttat gcgcacgccc agtgctggtg aaagtttcca gtctggaaac ggcatcttcg
    20941 tcggtaatga agtgcgtcat gttggcatat tcacccacca tcggcgtgag aggcaattgc
    21001 agaccgtgac ccaaatctac caccacgccg ccatcgccaa tctgcataat caaggtgccg
    21061 tcaggcgatg aaat
//
LOCUS       DADLTD010000055        20767 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLTD010000055 DADLTD010000000
VERSION     DADLTD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.055"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(17..562)
                     /gene="hemG"
                     /locus_tag="JN701_001629"
     CDS             complement(17..562)
                     /gene="hemG"
                     /locus_tag="JN701_001629"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY7364114.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(574..2025)
                     /gene="trkH"
                     /locus_tag="JN701_001630"
     CDS             complement(574..2025)
                     /gene="trkH"
                     /locus_tag="JN701_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY7364115.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2064..2678)
                     /locus_tag="JN701_001631"
     CDS             complement(2064..2678)
                     /locus_tag="JN701_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY7364116.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2678..4009)
                     /gene="pepQ"
                     /locus_tag="JN701_001632"
     CDS             complement(2678..4009)
                     /gene="pepQ"
                     /locus_tag="JN701_001632"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY7364117.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4199..6395
                     /gene="fadB"
                     /locus_tag="JN701_001633"
                     /pseudo
     CDS             4199..6395
                     /gene="fadB"
                     /locus_tag="JN701_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
     gene            6405..7568
                     /gene="fadA"
                     /locus_tag="JN701_001634"
     CDS             6405..7568
                     /gene="fadA"
                     /locus_tag="JN701_001634"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY7364118.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMRHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7760..8461)
                     /gene="fre"
                     /locus_tag="JN701_001635"
     CDS             complement(7760..8461)
                     /gene="fre"
                     /locus_tag="JN701_001635"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY7364119.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8507..10000)
                     /gene="ubiD"
                     /locus_tag="JN701_001636"
     CDS             complement(8507..10000)
                     /gene="ubiD"
                     /locus_tag="JN701_001636"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY7364120.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10167..10655
                     /gene="rfaH"
                     /locus_tag="JN701_001637"
     CDS             10167..10655
                     /gene="rfaH"
                     /locus_tag="JN701_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY7364121.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10652..11434)
                     /gene="tatD"
                     /locus_tag="JN701_001638"
     CDS             complement(10652..11434)
                     /gene="tatD"
                     /locus_tag="JN701_001638"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY7364122.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAVQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11476..12252)
                     /gene="tatC"
                     /locus_tag="JN701_001639"
     CDS             complement(11476..12252)
                     /gene="tatC"
                     /locus_tag="JN701_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY7364123.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12255..12770)
                     /gene="tatB"
                     /locus_tag="JN701_001640"
     CDS             complement(12255..12770)
                     /gene="tatB"
                     /locus_tag="JN701_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY7364124.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12774..13043)
                     /gene="tatA"
                     /locus_tag="JN701_001641"
     CDS             complement(12774..13043)
                     /gene="tatA"
                     /locus_tag="JN701_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY7364125.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13122..14762)
                     /gene="ubiB"
                     /locus_tag="JN701_001642"
     CDS             complement(13122..14762)
                     /gene="ubiB"
                     /locus_tag="JN701_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY7364126.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14759..15364)
                     /gene="ubiJ"
                     /locus_tag="JN701_001643"
     CDS             complement(14759..15364)
                     /gene="ubiJ"
                     /locus_tag="JN701_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY7364127.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15378..16133)
                     /gene="ubiE"
                     /locus_tag="JN701_001644"
     CDS             complement(15378..16133)
                     /gene="ubiE"
                     /locus_tag="JN701_001644"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY7364128.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16228..17655)
                     /gene="rmuC"
                     /locus_tag="JN701_001645"
     CDS             complement(16228..17655)
                     /gene="rmuC"
                     /locus_tag="JN701_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY7364129.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17796..18557)
                     /gene="udp"
                     /locus_tag="JN701_001646"
     CDS             complement(17796..18557)
                     /gene="udp"
                     /locus_tag="JN701_001646"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY7364130.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18819..19631
                     /locus_tag="JN701_001647"
     CDS             18819..19631
                     /locus_tag="JN701_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7364131.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20025..20293
                     /gene="hcp"
                     /locus_tag="JN701_001648"
                     /pseudo
     CDS             20025..20293
                     /gene="hcp"
                     /locus_tag="JN701_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
     gene            20447..20767
                     /locus_tag="JN701_001649"
                     /pseudo
     CDS             20447..20767
                     /locus_tag="JN701_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356833.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5084 a         5605 c         5209 g         4869 t
ORIGIN      
        1 tttttattct ttatgcttat ttcagcgtcg gtttgttggt taactgagcg atttctcggg
       61 cgaaattcgc cacctgctcc caatcggtat agacaacttc tttgcgcgta tccgtttcac
      121 cgcctgacat cttcataatc agcttaatca taaaacggtc gtaccagcga taacgtgggt
      181 aacgcagcgc cccggcaatg accgcgcagc gatcgggacg ccattgtgaa ctcatcagaa
      241 acttgcgcgc gtagctgttg gtctgtggag tacgcttctc cggtttgcgc gccaccaggt
      301 ttacggaata aaaggcgctc ggcatcgaat tcagtcgcgt cgcatgtttt ttgacaaatt
      361 cctggaacgc agagtggtag tgaccatagc gaatagaagc accaatgacc acacggtcat
      421 agttttccca ttgtggttct tcaatgcggt gcacattggc gacatccgtc tggatcccca
      481 gttctttcag ttccgaagcc aggtaggagg caatctcgcg cgtttgtccg tcccttgttg
      541 agaaaagaat taatgttttc acgtattact ccatcattca cgccagaaag tcggggtaaa
      601 gagcaccagc aatgtaaaga cctcgagacg accaaacagc atgttggcaa tcaggatcca
      661 tttagccacc gggttcatac tggtaaagtt atcagcaacc acgccaagcc ctggccccag
      721 gttattcaat gtcgcaacaa ctgaggcaaa ggcagaaaaa tcatccacgc ccgtggcgat
      781 aatcgccagc atactgacaa taaacaccaa tgcataggcg gagaaaaatc cccaaacggc
      841 ttcgaggata cgttccggca gtgcgcgatt ccccagctta atgctataga cggcgttcgg
      901 atgcaccagt cgtttcagct cacggttccc ctgcttaaac agcagcagga tgcggatcac
      961 tttcaggcca ccgcccgttg acccggcgca accgccgata aaagctgaac ataaaagcag
     1021 taccggcaaa aagagcggcc agcgggcaat gctgtcagtc gtaaacccgg cggttgtcgc
     1081 catcgatacc acctggaaaa acgcctggtt aattgtcatc agcgccgaac tgtagacatt
     1141 atgaaaccac agtacgagtg tacaaataac caccagcgta aactgtacgc cgataaacat
     1201 gcgaaattcc ggatcgcgcc aataaacctt cagactacgc ccacttaaca gtgaaaagtg
     1261 cagaccgtag ttacaaccgg agatcagcag gaagatagca atgatagtgt taatagtcgg
     1321 actgtcgaaa taaccgatac tggcatcatg tgtcgagaag ccgccaatag cgatagtcgc
     1381 aaagctatgg ccgatggcat caaaggcatc cattccggca aaccacaacg ccagcgcaca
     1441 ggcgacggtc agcaagacat aaatcaacca cagggttttc gccgtttccg caattcgcgg
     1501 gcgcattttg ttatctttca gcgggccggg catttctgcg cgatagagct gcatcccacc
     1561 cacgccgagg ataggcagta tcgcaaccgc taacacgatg atccccatcc cgccaaacca
     1621 ttgcagcatc tggcgataaa agaggatggc atgagggagc gaatccagcc ccaccagcgt
     1681 agtggccccg gtggtggtca ggccagagaa agattcaaaa aacgcatcgg taatcgtgag
     1741 gttcgggctt tccgagaaga taaaagggag cgcaccgaca ctacccagca cggtccaaaa
     1801 cagcaccact atcagaaacc cctcacggga tttcagttcg cccttctctt tgcggttcgg
     1861 ccaccacagc atagagccaa tggcgagggc gacaaaaaag gtctgggtaa aagcgcggcc
     1921 cgctccatcc cggtagatga gtgctaccag cccagggata atcatggtcc ctgaaaataa
     1981 gatgaccagt agtccaacga ttcgggtaat ggcgcgaaaa tgcatctctg ccgcttcctt
     2041 agttctgcaa aatgaagtgg ggattattct tcaatcgcta acaattgcaa tgaaccacgg
     2101 ctaaaatccg ccagctttgc ggaaaattca gccactttcg ccgccggaag cgccacccgc
     2161 agcagaacga atgcctgata atcactgttg ataattttgc cgtcacactg ccccagcaac
     2221 gcttcaatgc cggttaactg actatattca cattgcaaag tatattcggt taatggcgtc
     2281 ttgcgttggg tcgttagctg gcgcagcgcc tgattcacgc cgccgccata cgctttcact
     2341 aacccaccgg tgccaagcaa tatgccgccg tagtagcgca ctaccacagc ggtaatttcc
     2401 ccgatgccgc tgcccattag ctgggcgagc atcggtttac ctgccgttcc cgccggctcc
     2461 ccgtcgtcag agaaacccag ctgttgagaa tcatccggcg cacccgccac ccacgccacg
     2521 caatggtggc gggcatcggg gtgttctgcc cgcaccgatt caacaaacgc tttcgccgcc
     2581 tcaacgccat cggtatgcgc caacatcgtt atgaagcggc tctttttgat ctcttcaacg
     2641 accgtgaccg gtgccgcagg aattaaccag ctttccatca cgccagtttc agatcccggg
     2701 tcatattttc cacgttattt tcgtggatca ccacgttgtc ttcgatacga atgccgctga
     2761 acggtttcag tgcttcaatt ttctgccagt tgaagtgctt gctgaactgc ccttcacgcc
     2821 acggtgccag tagcgattcg atgaagtaga tacccggttc gatggttaac accatgcccg
     2881 gctggagaat acgggtgcag cgcaggtacg gatattttgc cggtgccgcg aggtgtgtac
     2941 cgctatcatc ctgcataaaa ccggctacgt catgcacctg caggcccagc ggatggccga
     3001 taccgtgcgg cataaacggt ccggtgagat cgttttcgac catcgcttct tcactcatat
     3061 cggtgatgat ttgatgttta cgcagcaatt tggcgatgcg ctgatggaac tggaggtggt
     3121 aatccacata gctgacgcca gctttcatgg tagcgatcag cgcaagttgt tcatcattta
     3181 cgtctttcac cagctgtgcg tagtcgttgt cgctttttgc cgaccaggta cgagtcaggt
     3241 cagccgcata gccgttatat tcggccccgg catccagcag gaagctgcgc atctcttccg
     3301 gtgcctgatg atccagtttg gtgtaatgca gcaccgaagc gtgttcgtta agcgccacaa
     3361 tgttgctgta aggtacgtcg gtatcacgat gaccggtcgc ggtcagatag gcgatgttga
     3421 tatcgaactc gctcatgcca gaacggaacg cttcttctgc cgcacgatga ccgttgaccg
     3481 ccattttctg cgcttcacgc atacaggcca gttcgtactc ggttttgaag gagcggtagt
     3541 aatgcaggta gtcgatcacc cctttcgggt tgatgttgct ggcctcaata cccagttgca
     3601 gcgcacgttc cggcaccgga ccgatataac cgatattgcc gcgcgcagcg ggcagcaggc
     3661 taccaatgcc atcggctttc ggcagtgcga tcacttccac atcttcagtc cagaaggagg
     3721 tcggcagcgg ttcgacgttg tgccagtaat caaccggcag atagaaccac agtttcggct
     3781 tgttcacgcc atccaccagc aaccagcagt ttggcacctg agttaccggc acccacgctt
     3841 tgaattgcgg gttcacttta aacggatagg gatgatcgtc gagaaaaacg ttgaacagct
     3901 cgccggagtg aataagtaat gcatccagct tgaagcgcgc cagcgcatcg cgagtccgtt
     3961 cttgtaaggt agctatatga tttttataga gcgaggccag tgattccatt ttttaccctt
     4021 ctgttttttt gaccttaagt ctccgcatct tagcacatcg ttcatccaga gcgtgatttc
     4081 tgccgagcgt gatcagatcg gcatttcttt aatcttttat ttacatgttt ttaacacaaa
     4141 atacacactt cgatgcatct ggtacgacca gatcaccttg cggattcagg agactgacat
     4201 gctttacaaa ggcgacaccc tgtaccttga ctggctggaa gatggcattg ccgaactggt
     4261 atttgatgcc ccaggttcag ttaataaact cgacactgcg accgtcgcca gcctcggcga
     4321 ggccatcggc gtgctggaac agcaatcaga tctaaaaggg ctgctgctgc gttcgaacaa
     4381 agcagccttt atcgtcggtg ctgatatcac cgaatttttg tccctgttcc tcgttcctcg
     4441 ttcctgaaga acagttaagt cagtggctgc actttgccaa tagcgtgttt aatcgcctgg
     4501 aagatctgcc ggtgccgacc attgctgccg tcaacggcta tgcgctgggt ggtggctgcg
     4561 aatgcgtgct ggcgaccgat tatcgtctgg cgacgccgga tctgcgcatc ggtctgccgg
     4621 aaaccaaact gggcatcatg ccgggctttg gcggttctgt acgtatgcca cgtatgctgg
     4681 gcgctgacag tgcgctggaa atcattgccg ccggtaaaga tgtcggcgcg gatcaggcgc
     4741 tgaaaatcgg tctggtggat ggcgtagtca aagcagaaaa actggttgaa ggcgcaaagg
     4801 cggttttacg ccaggccatt aacggcgacc tcgactggaa agcaaaacgt cagccgaagc
     4861 tggaaccact aaaactgagc aagattgaag ccaccatgag cttcactatc gctaaaggga
     4921 tggtcgcaca aacggcgggg aaacattatc cggcgcccat caccgcagta aaaaccattg
     4981 aagctgcggc ccgttttggt cgtgaagaag cattaaatct ggaaaacaaa agctttgtcc
     5041 cgctggcgca caccaacgaa gcccgcgcac tggtcggcat tttccttaac gatcaatatg
     5101 taaaaggcaa agcgaagaaa ctcaccaaag acgttgaaac accgaaacag gccgctgtac
     5161 tgggcgcagg catcatgggt ggcggcattg cttaccagtc tgcgtggaaa ggcgtaccgg
     5221 ttgtcatgaa agatatcaac gacaagtcgt taaccctcgg catgaccgaa gccgcgaaac
     5281 tgctgaacaa gcagcttgaa cgcggcaaga tcgatggtct gaaactggct ggcgtgatct
     5341 ccacgatcca cccaacgctc gactacgccg ggtttgaccg cgtggatgtt gtggtagaag
     5401 cggttgtcga aaacccgaaa gtgaaaaaag ccgtactggc agaaaccgaa caaaaagtac
     5461 gcccggatac cgtactggcg tctaacactt caaccattcc tatcagcgaa ctggccaacg
     5521 cgctggaacg cccggaaaac ttctgcggaa tgcacttttt taacccggta caccggatgc
     5581 cgttggtaga aattattcgc ggcgagaaaa gctccgacga aaccatcgcg aaagttgtcg
     5641 cctgggcgag caagatgggc aagacgccga ttgtggttaa cgactgcccc ggcttctttg
     5701 ttaaccgcgt gctgttcccg tatttcgccg gtttcagcca gctgctgcgc gacggcgcgg
     5761 atttccgcaa gatcgacaaa gtgatggaaa aacagtttgg ctggcctatg ggtccggcat
     5821 atctgctgga tgtcgtgggc attgataccg cgcatcacgc tcaggctgtc atggcagcag
     5881 gcttcccgca gcggatgcag aaagattacc gcgatgccat cgacgcgctg tttgatgcca
     5941 accgctttgg tcagaagaac ggcctcggtt tctggcgtta taaagaagac agcaaaggta
     6001 agccgaagaa agaagaagac gccgccgttg aagacctgct ggcagaagtg agccagccga
     6061 agcgcgattt cagcgaagaa gagattatcg cccgcatgat gatcccgatg gtcaacgaag
     6121 tggtccgctg tctggaagag ggcattatcg ccactccggc ggaagcggat atggcgctgg
     6181 tctacggcct gggcttccct ccgttccacg gcggcgcgtt ccgctggctg gacaccctcg
     6241 gtagcgcaaa atacctcgat atggcacagc aatatcagca cctcggcccg ctgtatgaag
     6301 tgccggaagg tctgcgtaat aaagcgcgtc ataacgaacc gtactatcct ccggttgagc
     6361 cagcccgtcc ggttggcgac ctgaaaacgg cttaaggagt cacaatggaa caggttgtca
     6421 ttgtcgatgc aattcgcacc ccgatgggcc gttcgaaggg cggtgctttt cgtaacgtgc
     6481 gtgcagaaga tctctccgct catttaatgc gtagcctgct ggcgcgtaac ccggcgctgg
     6541 aagcggcggc cctcgacgat atttactggg gttgtgtgca gcagacgctg gagcagggtt
     6601 ttaatatcgc ccgtaacgcg gcgctgctgg cagaagtgcc gcactctgtc ccggcggtta
     6661 ccgtcaatcg cttgtgtggt tcatccatgc aggcactgca tgacgcagca cgaatgatca
     6721 tgactggcga tgcgcaggca tgtctggttg gcggcgtgga gcatatgggc catgtgccaa
     6781 tgaggcacgg cgtcgatttt caccccggcc tgagccgcaa tgtcgccaaa gcggcgggca
     6841 tgatgggctt aacggcagaa atgctggcgc gtatgcacgg tatcagccgt gaaatgcagg
     6901 atgcctttgc cgcgcggtca cacgcccgcg cctgggccgc cacgcagtcg ggcgcattta
     6961 aaaatgaaat catcccgacc ggtggtcacg atgccgacgg cgtcctgaag cagtttaatt
     7021 acgacgaggt gattcgaccg gaaaccaccg tggaagccct cgccacgctg cgtccggcgt
     7081 ttgatccagt aaacggtacg gtaacggcgg gcacatcttc tgcactttcc gatggcgcag
     7141 ctgccatgct ggtgatgagt gaaagccgcg cccatgaatt aggtcttaag ccgcgcgctc
     7201 gtgtgcgttc gatggcggtc gttggttgta acccatcgat tatgggttac ggcccggttc
     7261 cggcctcgaa gctggcgctg aaaaaagcgg ggctttctgc cagcgatatc ggcgtgtttg
     7321 agatgaacga agcctttgcc gcgcagatcc tgccgtgcat taaagatctc gggctaatgg
     7381 agcagattga cgagaagatc aacctcaatg gtggtgcgat cgcgctgggt catccgctgg
     7441 gctgttccgg tgcgcgtatc agcaccacgc tgctgaatct gatggaacgc aaagacgttc
     7501 agtttggtct ggcgacgatg tgtatcggtc tgggtcaggg tattgcgacg gtgtttgagc
     7561 gggtttaacc acttgccgga tgcggcgtga acgccttatc cggtctaccg atcctgcacc
     7621 aatgtaggcc tgataagacg cgccagcgtc gcatcaggca tcgtgcaccg attgccggat
     7681 gcgacataga tctaaatgcc actgaataaa gttttagttg ccgttcttcc cgcctgtcag
     7741 gggcgggttt ttttatatct cagataaatg caaacgcatc gccaaacaga cgatcttccc
     7801 gcgcattacg ctcactgcaa aacaggtcac gagcaatttt cgccatctca aaacgtccgg
     7861 caatatagat atcatgctct gccagcgtac catgatcctg caagaccgcc gttaacacgg
     7921 tgccagtacg cccacgccag cccgcttccg gttgttcaac caccggcacc acacgcaggc
     7981 caggatgctt cagcgaaagc gcctcaagct cggagagatc ataaagatgt tgctcctcac
     8041 gcccacccca gtaaatggtg atatcgcgat tgggattacg cgccaacgcc gtcaataaaa
     8101 tcgagcgcgc ataggagaac ccggtgccgc ccgcaatcaa aatcatcgga cgctcttcat
     8161 catcgcgcag ccacgcttct ccgtagggaa tatcgaccac gatttgataa tctttgagga
     8221 tgcggtccat gactgctttc gcgtaaaggt tgatttcaga agcgccaata tgcagctcta
     8281 taaacccttt ttcatccggc gtcgaagcca ttgaaaacgg acgtttgtcg cgctcatcca
     8341 tcactaccat caaatactga ccagcacgaa aagaaaaggc cgcgtctggc acgatgcgga
     8401 cacgatatac ggtatccgtg atagcttcta ccgaggtcac tttacagctt aaggttgtca
     8461 tgcgctttct ctgtcggatc gataaatagg gcaaaacaaa cgcgcatcag gcgcttttac
     8521 cgttgttaaa aatagccagt tcatcccaga tggcgtcaat atgcgcgaca acatctggat
     8581 cttttttgat gggacgtccc cattcacgct gggtttcccc cggccattta ttcgtggcat
     8641 ccagccccat ttttgaaccc agcccggaga caggcgaggc aaaatccaga taatcaatag
     8701 gcgtattttc taccagaaca gtatcccgcg ccgggtccat acgggtggta atcgcccaaa
     8761 tcacatcgtt ccagtcgcgt gcgttgacgt catcatcgca aacgatcaca aatttagtgt
     8821 acataaactg gcgtaagaac gaccagacgc ccatcatgac gcgcttcgcg tgtccggcgt
     8881 actgtttttt gatcgtcact accgccagac gataagaaca gccttccggc ggcaggtaaa
     8941 aatcgacaat ttccgggaac tgtttttgca gaatcggtac aaacacttcg ttcaacgcca
     9001 cgcccagtac cgcgggctca tctggcggac gcccggtata ggtggaatgg taaatcgcat
     9061 cttcacgctg ggtaatatgc gtcacggtaa ataccgggaa actatcgact tcgttatagt
     9121 aaccggtgtg gtcgccatac ggcccttccg gcgcagtttc gccttgttcg atataccctt
     9181 ccagcacaat ctccgcactg gcgggcactt caaggtcatt ggagatacac ttcaccactt
     9241 cggtcttggt accgcgcagc aatccggcaa acgcatactc tgaaagtgta tccggaacgg
     9301 gggtgactgc accgagaatc gtggcgggat cggcacccag cgccacagaa accgggaaac
     9361 gttcgcccgg atgcgccgca caccactcct gataatccag cgcgccgccg cgatgcgaca
     9421 gccagcgcat aatcagtttg tttttaccaa tcagctgctg gcgataaatg cccagattct
     9481 gccgctcttt atgcgggccg cgcgtcactg tcagccccca ggtaatcagc ggtgcggcat
     9541 cttccggcca gcaggtcata atgggaatgc gattgagatc gacgtcatcg ccagagacga
     9601 ttttttgttg gcagggcaca ccacgcagcc gctttgtcgg catgttcaat acttgcttaa
     9661 actgcggcag tttatcaaac aggtcgcgga aaccttttgg cggctccggc tctttcagaa
     9721 acgccaataa tttaccaact tcacgcagcg ccgaaacatc ttcctgtccc atgcccatcg
     9781 ccacgcgctt tggcgtaccg aacaggttgc acagcaccgg cattgagtag cctttagggt
     9841 tttcgaacaa cagcgcaggc ccaccggcac gcaaagtgcg gtcagcaatt tcagtgattt
     9901 ccagatgcgg atccaccggg agcgtgatac gttttagctc accctgctgt tcaagcagcg
     9961 tcaagaagtc gcgtaaatcg ttatatttca tggcgtccat tgtagcctct taatctacgc
    10021 ccattatacg gcgttcatct ttgcgatgct gtaaatttgt taaattagcg tgaactctga
    10081 cggtataacg caaaccgggg aatataatta acttagcgta aagcttttgc tatccttgcg
    10141 ccccgattaa acggataaga gtcattatgc aatcctggta tttactgtac tgcaagcgcg
    10201 ggcaacttca acgtgcccag gaacacctcg aaagacaggc tgtgaattgc ctggcaccga
    10261 tgatcaccct ggaaaaaatc gtgcgtggaa aacgtactgc agtcagtgag ccattgtttc
    10321 ccaactacct gtttgtcgaa tttgatccag aagtgattca taccacgact atcaacgcga
    10381 cccgcggtgt cagccacttc gtgcgctttg gcgcgtcgcc agcgatagtc ccatcggcgg
    10441 tgattcatca gctatcggta tataaaccga aagacattgt cgatccggca acaccttatc
    10501 cgggtgataa ggtgattatt accgaaggcg cgttcgaagg ctttcaggcc attttcaccg
    10561 aacccgatgg tgaggctcgt tccatgctat tgcttaatct tattaataaa gagattaagc
    10621 acagtgtgaa gaacaccgag ttccgcaaac tctaaaacgc aatcccaaac agtgttttga
    10681 cattggcatc cgtggtggca gccagccatg cggcatcttc tccacgccag tgcgcaatac
    10741 gttgcaaaat atggtgcaga tgggctggct cgttgcgccg ggatgatggc tttggcgtga
    10801 aatcgcgcgg gagcagatac ggcgcatcag tttcgatcag caatttctcc gccggaatca
    10861 acggtaataa ttcccgcagc tccagcccgc gtcgttcatc gcaaacccaa ccggtaatgc
    10921 cgatataaat tccacgcgcc acgcacgcct gcatctcttc gcgtgtgccg gtaaagcaat
    10981 gaagaaccgc accaggcagt ttatccagcc acggctccag caatgtcata aaccgctcgt
    11041 gggcatcgcg acagtgcata aataccggca tgtttaattc tgcggcaatg cgtagctgag
    11101 caacaaaagc gcgttcctgc tcttccggcg tcgaaaagtt gcggttaaag tcgagaccac
    11161 attcaccaat cgccaccact tctggctgca cggccagctc aataatcgct tcttcagtcg
    11221 cagcttgcca ctggctgctg tcgtgaggat gtacgcccgc cgttgaccaa caggacgaat
    11281 actgacgcgc cagcttttgc gcctgctggc tttcacgcag gttagtgccg gtgatgagta
    11341 gcccattaac tcccgcgtca aaagcgcgcg ctacaacatc atcacggtct ttcgcaaatt
    11401 gcgaactggt caaattaacg ccgatatcaa acatcctgta ctccatatga caaccgccct
    11461 gacgggcggt tgaatttatt cttcagtttt ttcgctttct gcttcagcgt cgttttcctc
    11521 ttcccgattt cgccctttac caacgtaaaa gcgtgagaag aagacaccga tttcaaacag
    11581 acagtacatc gggatcgcca atagcgtttg cgagaagaca tccggcggcg tcagcaacat
    11641 cccgacaacg aatgcaccaa ccagcacata cgggcgtttt ttgcgtaagt cttctggcga
    11701 ggtaatcccc atccagcaca gcagcacaat tgccaccggc acttcaaagg agacaccaaa
    11761 cgccataaac agcgccataa cgaagcttaa atagctggcg atgtcggtgg atacctgcac
    11821 cccttccggc gcggtattgg caaggaagcc aaatgccagc ggaaagacca caaagtaggc
    11881 gaatgccatg ccgatataaa acagcagaga gctggaaacc agcagcggca ccaccaggcg
    11941 acgttcatgc ttatacagcg ctggggcgat aaatgcccac acctgataga gaatcaccgg
    12001 cgctgacaga atcagcgaca ccataaaggt cagcttgatc ggcgtaaaga acggcgaggc
    12061 cacgtcggtg gcgatcatcg ttgaaccttg cggcaactgc ttgatcaatg gcgcggatac
    12121 caggtgatag atgtcattgg cgaaatagac cagacacagg aatatcacga tcaccgcgat
    12181 aatgcagttc agcagacgct tacgcagctc aattagatgc gtgataagcg gttgagtatc
    12241 ttctacagac atgtttacgg tttatcactc gacgaagggg aaggtgcagc ggttttcggt
    12301 tcagcgtccg cagcaggttt taccaccggc tctggcgtgg tttctggctt ctgttccggc
    12361 gaactggcct gcgtttgtgc agcggcaggc gttacgccct catgcgcagc ttcattatct
    12421 ttcaccaccg ggttatggat ggtgtgcgct tcatcgctcg ccttttctgg atcgtttgca
    12481 acgtaggaac gcttcatcga ctccgcggcc tggcgtagtt catccatcga cgctttcagt
    12541 tcgggcgtca ggttagtgag gctcgccttt tcaacctttt tcagactgtc ctgaaactcc
    12601 tggagtttta actcctggat cagttcgttc tgcaccgttg tcgccagtga acgcaacgcg
    12661 cgaatccagc ccgctaccgt ttttaccgcc acaggcagtc gttgcggccc cagaacgacg
    12721 aggccgatga tgaacaccaa tagcagttcg ctaaaaccga tatcaaacac ggattacacc
    12781 tgctctttat cgtggcgctt cgcgtcttct gttttagcct gttcctgatt cgtatccgcc
    12841 tgcttatcgg cgatagtttt cgcagtaaaa tcagcatcct gactggtttt atcctgcttt
    12901 ggttcatcat cgctcattgc ttttttaaag cctttgatcg acgcaccaag atcggaaccg
    12961 atggagccga gctttttggt gccaaaaagc agtacaacga tgacggcaat aatcaataac
    13021 tgccaaatac tgataccacc catacatgtt cctctgtggt agatgatgat taaacaaagc
    13081 cgcattatac gttacacggc ccgccttgag cgatgaaaaa atcagcgtgt tttgcgccaa
    13141 ccgacaaacc aggcgatcag accacctgcc attaaccagc cgggcatcag cccccactca
    13201 ggtcggctga ccaacaagaa tgtgccactt aatactaacg tagcgccaat tccgagaaaa
    13261 taacgcgatt gtccctgacg tacatgattt gactgaagct cgcgggcaat cttatcaaca
    13321 ctgtgctgta aatacttgcc ctggcgcaaa ctgtcgtaaa ccaattcagg cagttctggc
    13381 attttttcga cccagaacgg cgctttttct ttaaatgctc tcaccagcgc aggaataccg
    13441 acctgatctt taatccacga ctccaggaaa ggcttcgctg ttttccataa atcgagctgc
    13501 ggataaagct ggcgtcctac cccttcgaca tagagcaggg ttttctggag taacaccagt
    13561 tgcggctgca cttccatatt gaagcgacgc gccgtattaa acagatttaa cagtacatgt
    13621 ccaaacgaaa tttcggccag cggtttctca aagataggtt cacagaccgt acgaatggca
    13681 aattcgaact cttcaacgtt ggtatctggt ggcacccagc ctgaatcgac gtgtagctct
    13741 gccactttgc gatagtcgcg attaaagaag gcgataaagt tttctgccag atagcgttta
    13801 tcttctttgt ttagcgagcc aacaatcccg caatcaatgc cgatatattt cgggttttcc
    13861 gggtgttcat agcttacgaa gatgttgcca gggtgcatat cggcatggaa aaagctgtcg
    13921 cgaaagacct gagtgaagaa cacctgcacg ccgcgttccg ccagcaattt catgttagtg
    13981 ccgtttttct ccagcgccgc aacatcagac accggaatgc cgtaaatgcg ctccatcacc
    14041 atcatccctt cactacaata gtcagggtaa acttccggga tgtagagcat cgggctgtct
    14101 tcaaaattgc gccgaagctg aatggcgttg gcagattccc gcagcaaatt cagttcatca
    14161 atcaaggtct tttcgtactc gcgcaccact tcggttgggc gcagacggcg accatccggc
    14221 agcaaacgcg gcacccagcg agccagacgg tagataagtt tcagatccgc tttaatgacc
    14281 ggcaaaatat ccgggcggat gactttaatc accacctctt taccattcga tttcaatcgc
    14341 gcggtatgaa cctgggcgat agaagcagaa gccagcggct tgatttcaaa atcgtcaaac
    14401 cacgcttcta ccggcaagcc gcccattgca gcttcaatct gctgcttcgc cagcttgcca
    14461 tcaaacggag ctactttgtc ctgcaataac gccagctgat cggcaatatg cggtggaaaa
    14521 agatcgcggc gggttgataa catttgcccg aacttgatcc aaaccggccc cagttcttgc
    14581 agggccagtc gtagtcgctc acctaaaagt ttgtctttat gccgatttgg catccagaat
    14641 aatgagtatc gccatagccg tagcggcagg gtgatacgca ttttggggat cagttcatca
    14701 agtccgtagc ttaaaaaagt gcgaatgatg aaatataggc gccgtacttc acctggcgtc
    14761 atttagcctc cagtttttcc agccgtttgg tcagggcatc aacagcacgc tcgacggcag
    14821 ccgtctcttc cgcaaaccag gccacttcaa gcggaccggg tgccatacgc cactcttcag
    14881 taatggcttc cgccacataa cgttgctggc gcttaatgcc gtgatgcagg aactttgcgc
    14941 ctccgcgcat ggctttgctg attccttcag cggcgatatc accggtataa ggggccagca
    15001 gttccgcagg gtcgaactct gccagatctg ccagcgcaac gaagttttgc accacctgaa
    15061 tatcgccctg cacttccagc tcaccactgc gaatcagtgc ggtaagctgc tggcgatcgc
    15121 gaagtttcgg caacacactg gcgtaggcga taacggtgca gtcagcatcg cctgcccatt
    15181 cgcccagtac atcaacctgg cgttcgctga acaccagaat caatgacgtc gaaaagcctt
    15241 ttacctccac gcgcaatact ttacccagca gacgcgagcg ggctgttttc agcgcgggtg
    15301 agcgatacag gaaggtgttg agcagacttt caattcctgc cgtcactaaa ggtttaaaag
    15361 gcatttccgg tctcctgtca gaacttataa ccacgatgca gcgccacaac ccctgccgtc
    15421 agattgtagt agtcgacact ttcgaatccg gcatcctgca tcatggcttt cagggtatcc
    15481 tgatcgggat gcatacggat ggattctgcc agataacggt agctgtcggc gtcgttcgcg
    15541 accagtgagc caatacgcgg cagcacatgg aaggagtatg catcataggc tttgctcagc
    15601 ggctcgataa ttggcttcga gaactcaagc accagcaggc ggccgccggg tttcagcacg
    15661 cgatacattg aacgcagtgc tttatctttg tcggtgacgt tacgcagacc aaacgaaatg
    15721 gtgatgcaat caaaggtgtt atccgggaac ggcagcgcct cagcgttcgc ctgaacatac
    15781 tcaacgttgc caatcacacc gatattacgc agcttctcgc gtcccatttt gagcatggat
    15841 tcattgatat cagcaaggac cactttgcca gtttctccga ccaggcggga gaatttcgct
    15901 gtcaggtcgc cggtgccacc agccagatcc agcacggtct gcccacggcg tacgccgctg
    15961 caatcaatcg tgaatcgctt ccacaaacga tgaataccaa atgacatcaa atcattcatg
    16021 acatcgtatt ttgatgccac ggaatggaaa acgtgggcga ccatatccgc tttttgttcc
    16081 ttcgcgacgg tctgaaaacc aaagtgcgtc gtttcttgtg acttatccac catctcaatg
    16141 cctgctcatc aaaaaattgt tccagaagtg taacagattg ggcgtcgatg ccctagattt
    16201 ctacccggct taactacccc taatgggcta gcgcgactgc tgattatatt catcatcgcg
    16261 ttgataagct tcatcattcg gctgctccgg aaccgaccga agtcgatact cttcatcctg
    16321 gctcaccgcc tgttcagcca aatccggatt aatctcgcgt ttaatttcta ctcctaaacc
    16381 gcgaaacgct tctgcctgcg ccagcacatt tccgcgacct gaagagagtt ttttcattgc
    16441 ctggcggtag ttatcctgcg ctttgtcgag actttgacca atcgcggaca tgtcatcgat
    16501 gaacaaacgc atcttgtcgt acagcttgct ggcacgatcg gcgatttgct gggcgttgcg
    16561 gctttgatgc tcataacgcc acaggttggc aatagtacgc aacgccacca gcagcgtagt
    16621 cgggctaacc agcatgatat tgtttttcaa cgcttcggtg atcagctccg gctggcggtc
    16681 aagcgccagt aaaaaagcgg gttcaacggg aataaacatc agcacgtaat ccagagttcg
    16741 cagccccggc agctgttgat aatctttgcg tcccagcaaa cggatatggt tacgcaccga
    16801 cgcgatatgt tcctgtagcg cgctttcgcg ggtgtagtcg tcttcggcgt taaaatagcg
    16861 ttcataggcg accagcgtca ttttggcgtc gatcaccaca tcttttccct gcggcagccg
    16921 cacgatgaca tccggctgca tccgcgagcg ggcgtcattt tcgatgctga cctgggtttc
    16981 atattcatac ccttcacgca gaccggaagc ctccagcacc cgcgtcaata ctacctcgcc
    17041 ccagttgccc tgggttttat tgtcgccttt cagcgcgcgc gtcaggttga tcgcttcctg
    17101 ggccatttgc gcgttgagtt gctggagatt gcgaatttcg tgggtcaggg tatggcgttc
    17161 ttgtgcttct ttaccgaagc tgtcctgaac ctgacggcgg aaaccgtcca gttgttcacg
    17221 tagcggcgac aacaggctgt tcagactctg acggttttgc tcatcaaccc ggcgattgct
    17281 gtgctcaaaa atacggttgg cgaggttttc aaactgctca ctgaggcgct gctcgctgtt
    17341 aatcatctgg cgaattttat cgtcagcatg ttgctgtgcg gcttccatcc gcgtggttac
    17401 ttcacgcaga tcggcctcca gagaggtgtt aatactttgc aggctgcgca cttcgttatt
    17461 gagtaactcg cactctgcac gccagtgctc gctttgggta atttgttgtt ttgccgcgct
    17521 taactccgcg accatctctt cacgttcagc taattgctcg gctttttgct gcgcatgttg
    17581 ataactggca aacagccagc caattgccac acccaccaac gcaataactg cgtaaaccat
    17641 gattgagaaa tccacaacgc ctcctgacgc aacaacttac ccgcacaaaa tagaattgtg
    17701 ctgtacaaac gtccagttgg aaaggcattt cctgcgaggc gctacgcaaa aatacaaaag
    17761 gccgaacgcg tcggccttca gacaggagaa gagaattaca gcagacgacg cgccgcttcc
    17821 accacgattt tcaccgcttg gctttcagtt tgtttcatcg tctcagcatt cgggatctct
    17881 tgctgggtgc ggttaacgat aacacccgct accataccgg cacgcaggcc ctgacttgca
    17941 cacatggtca gcagggttgc agattccatt tcatagttca ttacgcccat cgcctgccac
    18001 tcttccatag aacctttaaa gtgacgaact acgcgaccag agtaagtatc gtaacgttcc
    18061 tggcctgggt agaaggtatc agaagaagct gtcacgccaa cgtgagttgt cgcgccaatg
    18121 gatttcgcag cttcaaccag cgcagtcgta cattcgaaat cagcgacagc cgggaattcc
    18181 agcggtgcga agtgcaggct cgcgccatcc agacggacag acgccgtggt aaccaggaca
    18241 tcacccacat taatatgcgg ctgaatagcg cccgttgtac cgatacgcag gaaggtgcga
    18301 atgcccagct gtgccagctc ttcaacagca atagaggtag acgggccgcc gataccggta
    18361 gagcagacga taacaggttt accatccagc tctgcacgcc aggtggtgaa ttcgcggtga
    18421 gatgccagct taaccggctt atccatcagc gcggcgatct tttccacacg atccgggtcg
    18481 ccagggacga tggcaagcgt agccccttgt aaatcgtttt tagtgaggcc gagatgaaaa
    18541 acatcagact tggacatata caactcctct gtgaatcggt ttagtcagaa gaagcaaaaa
    18601 gacactttac cgaagggttt aacatttttt cgtgatactc atcaccatga cgcaaatgcg
    18661 ttgcataaat ttacccacca aaagtgatgc aaatcacata aatcactctt cattctgcag
    18721 gactgcataa aagatgagcc gattcaggca atgtgatttg ttgcatgctg actatagtta
    18781 acattgcgca acatttttat aagggtacgg agaataccat ggcaacaaca caacaatctg
    18841 gatttgcacc tgctgcatcg cctctcgctt cgaccatcgt tcagaccccg gacgacgcga
    18901 ttgtggcggg cttcacctct atcccttcac aaggggataa catgcctgct taccatgcca
    18961 gaccaaagca aagcgatggc ccactgccag tggtcattgt agtgcaggaa atttttggca
    19021 tgcatgaaca tatccgcgac atttgtcgcc gtctggcgct ggaggggtat ctggctatcg
    19081 cacctgaact ttacttccgc gaaggcgatc cgaatgattt tgccgatatc cccacgttgc
    19141 ttagcggtct ggtagcaaaa gtgcctgact cgcaggtgct ggccgatctc gatcatgtcg
    19201 ccagttgggc ttcccgcaac ggcggcgatg ttcatcgttt aatgatcacc ggattctgct
    19261 ggggtggacg tatcacctgg ctgtatgccg cgcataatcc acagctaaaa gccgcagtgg
    19321 cgtggtacgg caaactgacg ggcgacaagt cgctaaattc accgaaacaa cctgttgata
    19381 tcgcaaccga tcttaacgcg ccggttctcg gcttatatgg tggtcaggat aacagcattc
    19441 cgcaggagag cgttgaaacc atgcgccagg cgctgcgggc tgctaatgcg aaagcagaga
    19501 ttatcgtgta cccggacgcc gggcatgcat tcaacgctga ttatcgcccg agctatcatg
    19561 ccgaatctgc gaaagatggc tggcagcgta tgctggaatg gtttaagcag tatggtggga
    19621 agcggagtta gtttcctctt agtctatatt tgagatggtg agtgaacgag tgcgttttgc
    19681 tgccatctct gtcccccaaa aaataatcag tatgaattga ttggagatta cgactccgct
    19741 atttgacctt aagaaagtcg gcaagtcata gtcataaatg agtctcttca atggaagaca
    19801 ccttagagat ttaccatcta ctcccattca caaacaaaca ttaattatta tcaacacatt
    19861 cacattaatt ataaatcatc tcagaccttc ctgataaaaa gatattaaag tgaaacattt
    19921 tgtttttcac attttagttc atcactttta ggcgtaaata aaggtaaaaa aaacaacaat
    19981 actatttctc tttcacaaaa gaattaacaa aaggtttttt aaggatgagt gatattattt
    20041 acttgaccat aaccggagaa caacaaggat gtatttcctc tctttgtgga acgagcgcat
    20101 caataggtaa tcgctggcag attggccatg aagatgaaat cttcgcgttc tcgctctcta
    20161 atagtattac gaatgcaggc aaagagtcac aattgcatgg ccttagtttc tgtaaattct
    20221 tattaacgct attaacaata acgagcagtt gttcatggcg tttgacttct atcggatcaa
    20281 tagatttggt cgatgggaga aatattacaa cattcaactc cgcggcgcgc tcttatcagc
    20341 aatcaatcac ctgtttactg agaacaatct ggatacagaa gccatcactg tcagttatga
    20401 atatatactc tccaggcatc tgattgctaa tactgaattt agttatttgg catttcccga
    20461 taactacaac cgtctgttta taccacggcc aaaaactaaa gctggtaatg gttcaaaaca
    20521 cttaatagta aagcggttgg cagactgctt gctgctggcg gcatatacaa tgggaatatc
    20581 gaaggattca gggaaacagc agacaagttg gggggagatg cacctgctgg ttacgatcaa
    20641 tttatggata acaaagggtt actcatcgct ggtgcttcaa tagttgctgg agcagttctg
    20701 gggagaatga gactccctga tttggaatca cttgagcatt tcggagccag aggagcagta
    20761 tcaggaa
//
LOCUS       DADLTD010000056        20659 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLTD010000056 DADLTD010000000
VERSION     DADLTD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.056"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..876
                     /locus_tag="JN701_001650"
     CDS             <1..876
                     /locus_tag="JN701_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7364132.1"
                     /translation="DQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQ
                     YGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKG
                     FSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTN
                     IINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAED
                     GGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQP
                     KLRGNVQTAQREVAP"
     gene            873..1238
                     /locus_tag="JN701_001651"
     CDS             873..1238
                     /locus_tag="JN701_001651"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAY7364133.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRVDYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGQRASDES"
     gene            1330..1962
                     /locus_tag="JN701_001652"
     CDS             1330..1962
                     /locus_tag="JN701_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7364134.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGDFSIYRQGGTSTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKSVRP
                     VVLPGIRYAVMCVPTPLALAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(2050..3348)
                     /locus_tag="JN701_001653"
     CDS             complement(2050..3348)
                     /locus_tag="JN701_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7364135.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3831..5243
                     /gene="uxaC"
                     /locus_tag="JN701_001654"
     CDS             3831..5243
                     /gene="uxaC"
                     /locus_tag="JN701_001654"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY7364136.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5258..6745
                     /locus_tag="JN701_001655"
                     /pseudo
     CDS             5258..6745
                     /locus_tag="JN701_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6828..7379
                     /locus_tag="JN701_001656"
     CDS             6828..7379
                     /locus_tag="JN701_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY7364137.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7384..8628)
                     /gene="sstT"
                     /locus_tag="JN701_001657"
     CDS             complement(7384..8628)
                     /gene="sstT"
                     /locus_tag="JN701_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY7364138.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9244..10209)
                     /gene="alx"
                     /locus_tag="JN701_001658"
     CDS             complement(9244..10209)
                     /gene="alx"
                     /locus_tag="JN701_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY7364139.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10493..11497)
                     /locus_tag="JN701_001659"
     CDS             complement(10493..11497)
                     /locus_tag="JN701_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7364140.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     "
     gene            complement(11558..12241)
                     /locus_tag="JN701_001660"
     CDS             complement(11558..12241)
                     /locus_tag="JN701_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY7364141.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12318..12821)
                     /locus_tag="JN701_001661"
     CDS             complement(12318..12821)
                     /locus_tag="JN701_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7364142.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12906..14042
                     /gene="rlmG"
                     /locus_tag="JN701_001662"
     CDS             12906..14042
                     /gene="rlmG"
                     /locus_tag="JN701_001662"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY7364143.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14327..14641
                     /locus_tag="JN701_001663"
     CDS             14327..14641
                     /locus_tag="JN701_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY7364144.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14638..15054
                     /gene="higA"
                     /locus_tag="JN701_001664"
     CDS             14638..15054
                     /gene="higA"
                     /locus_tag="JN701_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY7364145.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15099..17117)
                     /locus_tag="JN701_001665"
     CDS             complement(15099..17117)
                     /locus_tag="JN701_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY7364146.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17543..19893)
                     /gene="ygjK"
                     /locus_tag="JN701_001666"
                     /pseudo
     CDS             complement(17543..19893)
                     /gene="ygjK"
                     /locus_tag="JN701_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19910..>20659)
                     /gene="ygjJ"
                     /locus_tag="JN701_001667"
     CDS             complement(19910..>20659)
                     /gene="ygjJ"
                     /locus_tag="JN701_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HAY7364147.1"
                     /translation="VPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMMNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRAD
                     GLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGG
                     RFATAFDSRFYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         5040 a         5412 c         5428 g         4779 t
ORIGIN      
        1 gatcaaacgt catggcagtt atcgcatacc ttttataatg attcattcag ccataaagaa
       61 cttggcgtaa ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt
      121 tgggatgggc aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat
      181 catgatcatc tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat
      241 ggcgttaatt tgggagccag tggtacagac gatttgctgg gtgcggtatt ggtggatgtg
      301 aaaggcttct ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca
      361 ggcagccgaa ctttgcagct tggtcaaagc gacagtgtgt tgttccctta tcctggattt
      421 cagtctggtt ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac
      481 atcattaacg gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc
      541 gaagtgtctg ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg
      601 aaaaaattcc cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga
      661 tttacacttg aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc
      721 cttaagtgtc cactgagtgt tgaaaagaaa cgcgccagca ttcgttacag cggagatgtt
      781 acttgtagtg tggtgactta ttcacaatta ccggagttga aacagcctaa attacgtgga
      841 aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat
      901 tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta
      961 cgtatgattt ggcatctggt agagtggatt attacttctg gaaggatgaa gcgtcggcag
     1021 gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag
     1081 ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt
     1141 ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgctag
     1201 gatgcaaggg ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt
     1261 acagaagatg gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag
     1321 cagacagaga tgaagaattt gccgattggt ggtgtctgga aggggaaagt taaattacat
     1381 tcgaacagcc cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac
     1441 catattgacg tgttcttccc ggagttcgcc cacgccacgc cgagggtgca gttagacttg
     1501 catccaacag gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg
     1561 tgcctgtacg atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa
     1621 gggcgaccag ctgcagggcg cagagacggt gacttctcta tctatcgtca gggaggaacc
     1681 tctaccgacg agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt
     1741 gggcaaattg atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaaagt
     1801 gtgcgtccgg tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg
     1861 gctctggcag tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa
     1921 ttgtcagtaa tctttacgcc ttccttgcca accatcaatt aaccctgcaa agcggacaaa
     1981 cgcttttttc tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta
     2041 cgttaaacgt taatgttgcg gtgcgggatc gttatgtgat tcctgcgcta cctcaatcgc
     2101 cggtttgttc tgcaacacgg tccagataac cagcgcacct aacaggtcga acactgccag
     2161 tactgcgaac agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgaaaa
     2221 cagcgtactt gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt
     2281 acgaccgaac acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgagcaaa
     2341 accaccgata cacagcagca taattgcgac atacgggttg gtgaacaggc cgatcatacc
     2401 cgggccaatc atcagcactg cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt
     2461 cacaccaaac caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc
     2521 agcaaacagc atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac
     2581 tttaaacatg aacagcggga tccacgcgtt aaaagtaccc caggctggtt ctgccaggaa
     2641 acgcggcagc gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt
     2701 cttcgccgtg ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc
     2761 atcggtcaga tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca
     2821 gataaagctc aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc
     2881 ccataccacc agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc
     2941 taccgcgatg gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg
     3001 gatcatcgct gcttccgcgg caccgactgc accacgagca atcgccaggc cacccctgct
     3061 accagccagc gcggttgcac cacagaatac ggcccacagt acagcaaaca ttgcataacc
     3121 gattttcgta cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc
     3181 agcagaatag gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat
     3241 cagagttggc gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag
     3301 cgtcaccagt gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat
     3361 cgttttgtta tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt
     3421 tacgaccggg taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg
     3481 ctaattcggc ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt
     3541 tgcattccgt cgacttatga tttgcgacgg cagaaagatg acttgtcata caactttaaa
     3601 aggtgagagc catcataaat gtgcgaatat ttgtagggac attacctgac gacagcgagg
     3661 ccagtactgg cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg
     3721 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     3781 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     3841 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     3901 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     3961 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     4021 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcgtct gaccgtgaaa
     4081 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     4141 cgcacctcga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     4201 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     4261 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     4321 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     4381 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     4441 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     4501 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     4561 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     4621 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     4681 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     4741 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     4801 gtccgatggc agaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     4861 cgaaaaccat tctgtactgc ctgaacccgc gcgataacga agtgttgggc accatgatcg
     4921 gtaacttcca gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     4981 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtttgctga
     5041 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     5101 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     5161 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     5221 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     5281 gctggataac gtcgcggtcg ctttagcgga tttggctgaa ggcacagaag tcagtgtcga
     5341 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     5401 tatcgcaaaa ggggccaatg tcatcaaata tggcctgccg attggttatg cattggcgga
     5461 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     5521 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     5581 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     5641 aaccgtgggc tgtgtcaacg gcatcgcgcg gcagatccag aaccgttttc tgaaagagac
     5701 caacaacgct gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     5761 gctgggcgac gatcacatta atacccgcac catgctgcaa aacatggtgc gccacccgaa
     5821 cgcaggcgca gtgctggtga ttggtctggg ctgtgaaaac aactaggttg ccgcattccg
     5881 tgaaacgctg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     5941 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     6001 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     6061 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     6121 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     6181 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     6241 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     6301 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     6361 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt
     6421 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     6481 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     6541 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggctcgact ttgacgcggg
     6601 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     6661 tgagtttgcc aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat
     6721 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     6781 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     6841 ggctggccca gggcgtgggt ttcatcgcct ttctgattgg tatcacaaca tttttcaatc
     6901 gtgacgaccg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     6961 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     7021 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     7081 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     7141 tcagtaccag ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     7201 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     7261 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     7321 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     7381 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     7441 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     7501 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     7561 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     7621 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     7681 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     7741 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     7801 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     7861 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
     7921 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
     7981 cgcgtaaccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
     8041 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacag
     8101 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
     8161 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
     8221 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
     8281 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
     8341 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
     8401 cccgtgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
     8461 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
     8521 tgagatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
     8581 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
     8641 tcattgtgtt gtcgcacatc ccaggtgcgt tctgtcgacg catctttcag gatgtaacaa
     8701 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
     8761 tttaaagaga ggtcatattt tgcaacttaa ttgttacatt tgtttgacat aaacagaggg
     8821 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
     8881 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
     8941 ggggcagcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
     9001 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
     9061 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
     9121 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccaattaca
     9181 taattaatag ctcacctttg tctggaatga gttatctcta ggaatggctt aatatacagc
     9241 gaatcattct ccccgctgct tatcatgccg ataattcacc ctggcgttga taataaacgt
     9301 catcaccaga atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc
     9361 gacaatcagc atcttgatac cgataaacac caggatcacc gccaggccat atttgagcat
     9421 cgagaaacgc tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc
     9481 aaacaggttt gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat
     9541 gctatccacg gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg
     9601 tgtggcgtac agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt
     9661 catgcgcaaa tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc
     9721 ttcatgggcg agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag
     9781 gatccagtcg aactgcgaaa ttagccagct gccagtgaag atcatgatgg tacgcagaac
     9841 aatcgccccg agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa
     9901 atagctgaac aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag
     9961 ataacccgtg agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cggtttgcac
    10021 cagataccac cagaaagcgg cgttaaacag taacgacagc gtcacccaaa ccagcgacca
    10081 ggccgcagcc tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc
    10141 gatagccagc ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac
    10201 agtattcata gaagttcctt acacataaaa aaaacggcca acgtcggaag acgtcagccg
    10261 ctgcttttta tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaaggaat
    10321 acgtctgcgt attcctttcg ggttgcccgg tgaccggatg tggtttttca cacatcgtaa
    10381 tgacgatcga ccggcaatga agttactccc ctttgcaggt aacaaagtat gacagaccat
    10441 tcagccagtc aatggtctgt tacatacctt ttacactgtt ttactttggc aattatagtt
    10501 ttacgctatc tgccggaaaa atcaccccag tctggcggcg aatctcagtc agcagtttgg
    10561 cggtgatgcg actgaccgcc agccctggat gatccaccag atgctcatcc accagggtag
    10621 caaacagctc tgcttcgtag agcatggtat taatatgctg cggctgggtg agatcctgca
    10681 tctggctgcc acgcggcacg aagcacactt tctggcattc agacagtttt tcaatcacca
    10741 gcgatcccgc ttcgccctga atctcgcttt ccaggacaga atcactgacc tttgagtgca
    10801 gcaacgtaac gctgaaatca ccgtaatcca tcaccaccac gccgtgggca tctacgccgc
    10861 ttgccaacaa actggcggtt gcctgcacgc ttttcggttc accgaataat gccaccgccg
    10921 acgccaggca gtaaaagccg atatccataa ttgaaccgtt agagaatgcc ggattaaagg
    10981 tgttgggatt ctcaccatcc aggtaacgtt gataccgcga ggaatactgg caatagttga
    11041 aaaagacttt acgcaatttg ccgactttcg gcagcgcctg gcgcagcaaa tgaaagttcg
    11101 gcaggctggc ggttttaaat gcctcaaaca gcaccacctg attttcccgc gcacaggcaa
    11161 tggcggcatc cacttccgcc agattcgacg ccagtggttt ctcgcaaatc acatgaattt
    11221 tatggctaag gaaaagttgt gtctgggaaa agtgcagggc attcgggctg gcaatataca
    11281 ccgcgtcaat ggcatcgctt tccgccatcg cttccagcga ggtaaacaga tgctcgacag
    11341 aaaaatcatt ggcgaagtgc tgggcctgtt caaggctgcg ggaatatacg gcggttaact
    11401 tgtatttacc gctctcatgg gcggcctcga cgaactggcg agtgatccag ttcgtaccaa
    11461 tcacagcgaa acgtatcata acgcgtggat actccataaa gggattctgt cgtcaatgta
    11521 gcacgccttt ttcatcttcg acgtgcgcag atacgcatta ttcttttgcc ggacagccat
    11581 gctcactaaa cggcccaatc atgaccgatg gcccgagata tttcggctgg ctatggagga
    11641 ttttggcgtc aatcacggcg ctgacacgag cgagtttctc tcgcagttga gtataaaaac
    11701 cgtgcttcat attgaggtca ggcgcgttaa aaccgatagc gtcgatgctg tactgttttg
    11761 ccagccagat ggcgcgttgg ttatgaaatt cttgcgagat aatggtgata tggttttcac
    11821 caaaaacctt tttggcacgc actaccgaat ccagcgttga gaatccggca tagtcgcaga
    11881 agatcacttt cgccggtacg cctttagcga tcaaagcctt ctgcatacct gatgcttcat
    11941 cgtaattttt acgtccgtta tcgccactta ccagtagcca tttcactttt ccggcgtggt
    12001 acaacgctgc tgcggtatca atacgtcggg taaagtagcg attacccggc ctggccccca
    12061 gcaataagcc cacgttacgc gccggaactg cgttgacatc gctccaggtc agctgcttgc
    12121 tggcgttgac cattactcga tcggcgataa aaatggtcgc tcctgccaca agtaacagta
    12181 agcaggcaat ctttagagtc cggcgagaaa aacaaatacg gagaagaagg cggggctgca
    12241 tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaaga aagaaaagat
    12301 aacgcctgac atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc
    12361 cgggtgtcga actccagctg gtggtactgc ggctccatat ggcagcacaa ctggtaaaac
    12421 gctttgttat gctctttctc cttcaggtgc gccagttcgt gcaccacgat catacgtaaa
    12481 aacggttccg gcgcgttgcg aaacacagta gcgacgcgga tctctgcttt tgctttcagc
    12541 ttgccgccct gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg
    12601 atcttattgt catacatcac tttattgatc ggcggggcat tgcgcagaaa ctgatttttt
    12661 agatcctgag tatattgcca gagggcttta tcggtggcgt agtcgtgtgt tcccggatag
    12721 cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt
    12781 agctgctcgg gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag
    12841 aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag
    12901 ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga
    12961 cggatgacgt taacccgcta caggcgtggg aagcggcgga tgaatatttg ctgcaacagt
    13021 tggacgacac agaaatccgc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa
    13081 gctgcgcgct ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg
    13141 cgacgcgcga gaatttacgc ctcaacggga ttgatgagtc gagcgtgaag tttctcgaca
    13201 gcaccgccga ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg
    13261 cattgctgga acaacaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta
    13321 ttgccggtgc caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc
    13381 tcggcccgac caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca
    13441 atgaaccgcc gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact
    13501 ggactatcca taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct
    13561 tctttatgca acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta
    13621 atggcgttat tggtctgacg ctgcttgata aaaatccgca ggcgaaagtg gtgtttgtcg
    13681 atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg
    13741 cgttggatcg cagcgagttt atgatcaaca acgcactctc cggcgtggag cctttccgct
    13801 ttaatgctgt gctctgcaac ccgccgtttc accaacaaca tgcgctgacc gataacgtcg
    13861 cctgggagat gttccaccat gcccgccgct gcctgaaaat caacggcgag ctgtatattg
    13921 ttgccaaccg tcacctggat tacttccata aactgaagaa gattttcggc aactgcacca
    13981 ccatcgccac gaataataaa tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt
    14041 aagattggga gttacctgcc ggatgcggcg ttaacgcctt atccggccta cgttcagcac
    14101 gttactccca ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc
    14161 tggaatcgcg cttccataaa acaaaaaatc cctctcacca tcctctacat tctcttgttt
    14221 agcgtttttc tacgtttatt cttccgtcac acagataaat tcatacgttg ccaatctgtc
    14281 aacggatgtt atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac
    14341 tcaaaaagca ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc
    14401 tctggggaac acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt
    14461 cccatctctg gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa
    14521 tgaattacgt gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt
    14581 tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg
    14641 attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgcggtggt accttttctg
    14701 gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg
    14761 ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa
    14821 gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata
    14881 gccgtgattc gtacccttat ggatcaatat ggtttaaccc ttgccgatct gccggaaatt
    14941 ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac
    15001 gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac
    15061 gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt
    15121 gttccctggg caattgcccg tcgtgcgttc agctccatag ccacatcgca gccgccaatt
    15181 aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc
    15241 cctgcgcaga tcaccacatt gtccacagct aatacctgcg tttcgccgtt gatcaccaca
    15301 tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc
    15361 gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg
    15421 ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc
    15481 gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg
    15541 gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca
    15601 atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca
    15661 tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac gcgcggcacg
    15721 atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga
    15781 ttgagtttca gcgtcacgcc cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg
    15841 taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc gctctcgctg
    15901 tgggcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc
    15961 ccagcaggtc ccgcaccgac caccgccaga ttttttttct gcacggcagg aaggattggc
    16021 attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag
    16081 atctgatcga ggcaggcctg attgcagcca atgcaggtgt tgatctcatc ggctcgcccc
    16141 gattgcgctt ttgacagcag ttccgcatca gcaagaaacg gtcgtgccat cgataccata
    16201 tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg
    16261 gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg
    16321 cgcggcacgg gcgtggcaat ggtcggaata cgtgcttcat gccagccaat gccggtgttg
    16381 ataatggtcg cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc
    16441 ccgccgtctt cgaccaggtc gagcatcgac agtcggtaga taataatgaa gtcgttgccg
    16501 acgcgttcgc gcaccgcacg cactacttct acggcaaatc gcatccggtt gcggtaatcg
    16561 ccgccccact ggtcactacg ctgattggtg tgcagcgtca gaaattcgtt gatcaaatac
    16621 ccttcggacc ccattacctc tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag
    16681 tgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa
    16741 cggttgatgg gggcctgcaa tgcggaggga gcgaccagat gcggttggta gctgtagcgc
    16801 ccggtatgca aaatttgcag ggctattttg ccgccttcct gatgtaccgc ttcggtaatg
    16861 gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg
    16921 cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt
    16981 tcggcataaa acgctgccag ccgctcggca ccgtccgggt attcctccag cccggtgtgc
    17041 attgagccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac
    17101 agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga
    17161 gttctaattt agccgtcgcc ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga
    17221 ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc
    17281 ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg
    17341 tgcagatgcc ggatgcgacg cttgtcgcgt cttatccgac ctacgggaac gcacatgtag
    17401 ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg tgcagttgcc ggatgcaacg
    17461 cttgccgcgt tttatccggc caggaggatt gcacatgtag ggcggataag gcgtttacgc
    17521 cgcatccgcc agcagttgca aattattgct tacggaaaaa atcgttatac aacatataca
    17581 aatgcgcggc actccaggag aaatttggtg cgccttgctg cgcgccagtc agcgggttgt
    17641 aattctcctg aattgggcca tcggcggtta atcctttggc gtgccggaag aacgtatccg
    17701 ccagcttcag ggcatcatca cgataaccgt aacgctccat ccctttcaga gcaaaccaga
    17761 actgatccac ccatacgcgc ccgcccagta gatatcagcg ccaaaagccg gattggttaa
    17821 cgccgccgtt cccagcggga caaaggtgtt gaactcttta ggatcgagca tcaccttcac
    17881 caccgcgtcg gcattggcct gcgttgccgc accgttaaac agcggcgacc agccttccgg
    17941 ccctttaccg cgctcaacaa tcggtttgcc cgcgcagccg ttcgccagtg gtttatcttc
    18001 aatacgtacg tcatagtaga actgggtagt cgggtcgaac atacaggtgt tgatgtagtc
    18061 cgcgagctgc tgtgccaact ggcgatagcg tttggcctct tctggcttac cgaggatggt
    18121 tgccatctcc gccagataat ggttatcgct gtacatatag ctggcctgat ccaccgactc
    18181 ctgcaatagc gagtagccca gcaacgttcc gtcctgactg cggttttcgg cgaatttcac
    18241 cgtccagtcg ctacgtttgc caccattagc gacatattta tccaactgtt ctttgtcgat
    18301 aaaaccaaag acggcggcat catcacgccc cgattcccac gacgcagcga cctgtgccgg
    18361 aatttccaga ctgtcgtact ggcctttctc caccacgcgg gcgtagttgt tcagcccaga
    18421 ctgcgtctct tctttgttgc ctttcttcac cgcaaacagc atctcgccgc tctcagtgtt
    18481 gtgggctttg tcgcgggtcg cgccatattc cggcacgccg ttgccgttgt gatcgcggtt
    18541 acgtaaccac cagtcgtgat aggcgaccag tttcgggtac atctctgcca accaggcttt
    18601 atcctgggtg acgttgtaca cttctatcac cgatcaggcg gcaaggctgg gtttggtatt
    18661 acgttcgttc cagttgccgc catcgccacc acgctcgggg ctaaggttcc acgctatcag
    18721 gtcggggaca aagcccacat cctgcggacg cacgcggtcg ccaggctgga tctgccagga
    18781 gaagaccgcg cggatattct ctttggcgat gtccggattg aaatgcgcca tcgcaaacgc
    18841 ctgcttctag gtatcccacg gccaggtctg attgccggag aaccagcgcc cggtcaccga
    18901 cggtgtaacg gtgttaaatt tcaccgcacc gcccggtgag cgccagttac cgttgagcgt
    18961 ttcgatcgct ttcaccgcga cgcgcgtctg ttccggcgtc gcatccggat tggttaaccc
    19021 tttcttcagg tattcttccc agcgttgctg cgaggcggtg agataaaacg ccggacgcgc
    19081 cagaatatcg cggatctgca tttgctcttt gctaacttcc tgagcggtca gcagatggga
    19141 ataggtggtg tagagcgtgg tcgaaccgtt gatatgcgcc ttactggtaa agcgattacc
    19201 gttgatttca gtctgcaccg gcagggattt atgcacctga tattccgatt cgccggaggt
    19261 cagcagatcc caggtggcgc gcactttgcc aaaggtgact ttcaggccat cacgggtggc
    19321 gctgattttg cgctgatagt cagggtattc gccagcaatg gttttatcgg aaagcggttt
    19381 cccttctttc gcttccagtt tttccagcag ttcgccgtcc cacaccagat ccagcggttt
    19441 attgctggtg attttggttt ccagtagtga cgtgcgcggc gtggcgaagc gcagagtcat
    19501 ttcgacttgc acatcttttg ctgtcagttt ttgcaccagc gcaccgggaa tactgtatgc
    19561 ctccagcgta aagtcgactt tcttgccgtc ctgccagacg gtcaggcggt cgaaattgct
    19621 ggccataaag ttgatgtact cttccgtcag cagcgcaacg cccggaaagc cgcccatggt
    19681 gttagggccg tctggcaaca gatgaccatg ccaggctccg agatcgaaaa acggattaaa
    19741 acgctggtga tcgtcgtaat cgtaatcctt catgtactgc ggcgcgccag tacggttaat
    19801 cacgttttta taattgtcgg cgttagcggc atgggcggaa aaacttatca gcagagcgca
    19861 ggttactggc gttaaaatag tttttatttt catgaaatta cagtccttat taccagtaga
    19921 aatattgcat caggaagatt tggtcgctgg gatcgtgaga ggcatttttc atgtagaagc
    19981 ggctatcaaa agccgtggcg aaacgtccgc cgccgtattc gtaccagata ccaaactgca
    20041 tgaaagaggt ggtttcatcg tcagcgtggt cagggcgatg tttggcgtag gtgtaagcgg
    20101 tggagagata aacgcccttt aacgcttcat acatggcgct caccatcatc ccactttcac
    20161 ttcccggtac gtccagcccg tcggcgcgtc cggtatggtg ccaggcgcga gcggcaaagt
    20221 taggagttag caggcccgtg agatagagct gtcctgtgcc gtcggtgatt tccagaccgt
    20281 tcatccaggt gatgccttcg gcaaggtcat actggatgta accgttcatc attgccggat
    20341 aggtgtattt gtcgtcatag cgatcgtatt tgccaaaatg tagccaggct ttgctttcat
    20401 catgatgtcc ggcaggagtc gcggtgacgc tataacgaaa gttgccgctt aagttctgta
    20461 ccttcagcgc agtcataatg tcgcgggtat tgggaatgac gtagcccaga tcgggggtga
    20521 aatcgcccca ccattgcagg tcatccagcg aggaatcctg acgcagactg agcatgattt
    20581 cggtgccatc attggtacga taaccgccat agaaacggtt aattccgccc tcgaaaccac
    20641 cccagctatg gtctggcac
//
LOCUS       DADLTD010000057        20603 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLTD010000057 DADLTD010000000
VERSION     DADLTD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.057"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(56..481)
                     /gene="nudI"
                     /locus_tag="JN701_001668"
     CDS             complement(56..481)
                     /gene="nudI"
                     /locus_tag="JN701_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY7364148.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            760..1302
                     /locus_tag="JN701_001669"
     CDS             760..1302
                     /locus_tag="JN701_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364149.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1402..2604
                     /locus_tag="JN701_001670"
                     /pseudo
     CDS             1402..2604
                     /locus_tag="JN701_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2824..3606
                     /locus_tag="JN701_001671"
     CDS             2824..3606
                     /locus_tag="JN701_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAY7364150.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3621..4826
                     /gene="rhmD"
                     /locus_tag="JN701_001672"
     CDS             3621..4826
                     /gene="rhmD"
                     /locus_tag="JN701_001672"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HAY7364151.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4883..6172
                     /locus_tag="JN701_001673"
     CDS             4883..6172
                     /locus_tag="JN701_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7364152.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6190..6993
                     /gene="yfaU"
                     /locus_tag="JN701_001674"
     CDS             6190..6993
                     /gene="yfaU"
                     /locus_tag="JN701_001674"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HAY7364153.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVSSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVLVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7034..7936)
                     /locus_tag="JN701_001675"
     CDS             complement(7034..7936)
                     /locus_tag="JN701_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7364154.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="JN701_001676"
     CDS             complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="JN701_001676"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY7364155.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="JN701_001677"
     CDS             complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="JN701_001677"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY7364156.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="JN701_001678"
     CDS             complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="JN701_001678"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY7364157.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12466..13824
                     /gene="glpT"
                     /locus_tag="JN701_001679"
     CDS             12466..13824
                     /gene="glpT"
                     /locus_tag="JN701_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY7364158.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13829..14905
                     /gene="glpQ"
                     /locus_tag="JN701_001680"
     CDS             13829..14905
                     /gene="glpQ"
                     /locus_tag="JN701_001680"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7364159.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14947..15189)
                     /locus_tag="JN701_001681"
                     /pseudo
     CDS             complement(14947..15189)
                     /locus_tag="JN701_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15368..16018
                     /gene="inaA"
                     /locus_tag="JN701_001682"
                     /pseudo
     CDS             15368..16018
                     /gene="inaA"
                     /locus_tag="JN701_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="JN701_001683"
     CDS             complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="JN701_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY7364160.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16326..17456)
                     /locus_tag="JN701_001684"
     CDS             complement(16326..17456)
                     /locus_tag="JN701_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase 1 subunit
                     beta"
                     /protein_id="HAY7364161.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="JN701_001685"
     CDS             complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="JN701_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY7364162.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4844 a         4992 c         5525 g         5242 t
ORIGIN      
        1 aattcgttaa ccactaaatt ttcaatctga cagccagtga tggctgttat cattgttaaa
       61 gaagaccttt caaacgtaac gtttttcggg tggcaacatt caaatcataa tgcaccagat
      121 cttcaggttt tacccacgtg tagtcctgaa actcttcgtt tattttcact tctcggttgg
      181 cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga ccatctgcat
      241 acgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc aaaagcagct
      301 gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt tcgccatact
      361 ccacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg tcggccattt
      421 tgcacagcag ataagcacca tcattttgaa tcaaagggca tacaatagtc cgttgtcgca
      481 caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg
      541 aaagtgacag taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc
      601 ggtaaagtgt gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag
      661 tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca atcccgcagc
      721 tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca
      781 ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggtcag
      841 gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggcg
      901 ttaagcggta actggacaca taacgacgac gacggtgacg tcgctggcgt ggggctgggg
      961 ttgaatctgc ctctagggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac
     1021 ccgaattacg gcgatgaagg ttatgccgca gcggtaggag gtggtttgca gtggaaaatt
     1081 ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt
     1141 attcaaagtt atgaggaagc gaatgctggc gcgcgttaca ccattatgcg tccagtcagt
     1201 attgaggcgg gttatcgcta cctgaatttg tcgggtaaag atggtaaccg cgacaacgcc
     1261 gtggctgacg gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat
     1321 tctgtcgcgc cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga
     1381 taacaaaagc aggagataac catgttaaaa gtggaaatgt tatccaccgg ggatgaagtg
     1441 ttacacgggc aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag
     1501 gggctgccat tatctcgcct caatacggtg ggggataacc ttgatgactt agtcaccatt
     1561 cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc
     1621 agcgatgatt taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat
     1681 gaagcctggc tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg
     1741 agcaaccgta aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc
     1801 accgcctgtg gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta
     1861 ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc cgcgcctgcg cgagcgtttt
     1921 tctttaccac agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat
     1981 ctggcacaaa gcctggacac tctacaactg ccgccgggcg taacaatggg ctatcgctct
     2041 tcaatgccga ttatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa
     2101 aaactgtggc tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa
     2161 ggactgcccg tgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc
     2221 gagtaattca ccggtggttt attggctttg caactttctc gcgcaggtgc tccattgctg
     2281 gcgtgtgaag tggttccttc acaggaagaa aacctggcgc aaactgcgca ctggattaca
     2341 gaacggcggg ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgaa
     2401 catctcaact ttgcgctagc cacaccagac ggcactttcg ctctgcgtgt gcgtttcagc
     2461 actacgcgct acagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg
     2521 ctgcgccgtt ggttaaacgg ccaggatatc gccagtgagc atggctggat tgaggttatt
     2581 gagtccatga ccttatctgt ctgattccgc cactctgtgc cggtttactg caccggcaca
     2641 ttccccctcc ggcaaaactt gtccaacctg ttgcactcaa taagaaaaaa tccgtgcact
     2701 gattcacaaa tttgtgcgta atttctccag tccgctggac gaggagcggg atttgatgcg
     2761 cttcacaatt ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc
     2821 agtatgttgg aatcaagcaa agttcccgcc ctgacacggg ctattgatat tctcaatttg
     2881 attgcccgca ttggcccctg tagtgctgcg acgatcattg acacattggg aatccctaaa
     2941 agcacggcct atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac
     3001 caggaaaatt tttgtttgtg gaccaggctg gtggagcttt ctggtcatgc gttaagcaag
     3061 atggatctcc gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg
     3121 ttatgtcatc tggggattat tgataacggc agcgcttatt acattttaaa agtggagtca
     3181 tcagccacta tcagcgtgcg ttcccatgaa ggaaaaagcc tttcgcttta tcgctccgga
     3241 ataggtaaat gcctgctttc ctggcaacct gcagcggtac agcaaagtat tatcgaaggg
     3301 ctggtctggg agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa
     3361 ctggcgcgta ttcgccgtca gggctggagc tatgacaacg gcgaagatta ccccgatgtc
     3421 cgttgtgtcg ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg
     3481 gtcggtaccc gcttacaaat caatgaagaa taccgtgatt atctggcggg caagtccatt
     3541 gcctgcgcca gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc
     3601 tcataacaat ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta
     3661 ctggcggtgc gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga
     3721 atcactggat agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt
     3781 cacgccagtc atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa
     3841 acggccagac cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa
     3901 aacaccttaa ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccacgatc
     3961 aaatgctcaa tgccaccctg tattactccg gctctggtgg cttggtgatg aatacgattt
     4021 cctgtgtcga tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata
     4081 aacttttagg cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg
     4141 atctggcaaa agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg
     4201 atggcgatgc ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg
     4261 gtgaagattt ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca
     4321 aactagccca cgcttgcgcg ccctataacc tgaaatggat cgaagagtgc ctgccgccac
     4381 agcagtatga aggttatcgc gaactgaaac gcaacgcgcc agtcggaatg atggtcacca
     4441 gcggtgagca ccacggcaca ctgcaatctt ttcgtacgct ttcagaaacc ggtatcgaca
     4501 ttatgcagcc ggatgttggc tggtgcggtg gtttaaccac gctggtggaa attgccgcaa
     4561 tcgccaaatc cagggggcaa ctggtggtgc cgcacggttc gtctgtttac tctcaccatg
     4621 cggtgatcac cttcaccaat acgccattca gcgaattcct gatgaccagc ccggattgtt
     4681 caacgatgcg tccgcagttt gacccgattc tgctcaatga gccggttccg gtgaatggtc
     4741 gtattcataa atcagtgctt gataaacccg gttttggcgt cgaactcaat cgtgactgca
     4801 atttgaaacg cccctacagc cactaatcac tccgggcgtt gctgtgacaa cggtaacgcc
     4861 cacatattct aaggagcttt ttatgagcac cgctttgctt gacgccgtgg tgaagaaaaa
     4921 ccgtgtgcgt ttaattccgt ttatgttggc gctgtatgtg ctggcgtttc tcgaccgttc
     4981 gaatatcggt tttgccaaac agacctacca gattgatacc gggttgagta atgaagctta
     5041 tgcgctggga gcaggcattt tctttgtggt atatgcgttt ctgggtgttc cggcgaatct
     5101 tttgatgcgc aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt
     5161 tctttcggca gccatggcat gggccgatac tgaagcgaaa tttctgatag ttcgcactct
     5221 gcttggtgct gcggaggccg gatttttccc tggtatgatt tatctcacct cgcaatggtt
     5281 tccgcagcgt aatcgcgcca gcattatggg gctgttctat atgggcgcac cgctggcgtt
     5341 aacactggga tcaccgcttt ccggcgcgct gttggagatg catggattta tggggcatcc
     5401 cggctggttc tggatgtttg tgattgaagg attgttggca gtcggtgctg gggtattcac
     5461 attcttttgg cttgatgaca caccggagca ggcacgtttt ctgagtaaac aagaaaaaac
     5521 gttgcttatc aatcaactgg caagtgaaga acaacagaaa gtgacttctc ggctgagcga
     5581 tgcgctgcgt aatggccgag tctggcaact agcgattatc tacctgacca ttcaggtggc
     5641 ggtttacgga ttaattttct tcctgccgac ccaggttgcg gcattgctgg gaacaaaagt
     5701 gggctttaca gcgtcggtgg tcaccgccat tccgtgggtt gcggccttgt ttgggacctg
     5761 gcttattccg cgctattccg ataaaaccgg cgaacggcgt aatgtcgcag cgctgacatt
     5821 actggcggcg ggcattggta ttggtctgtc cgggctgctt tctccagtaa tggcgatcgt
     5881 agcgctgtgt gttgcagcta tcgggtttat tgccgtgcag ccggtgttct ggacgatgcc
     5941 gacacaactg ctttccggta cggcgctggc tgcggggatt ggttttgtaa acctgtttgg
     6001 tgcagtgggc gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tatttgccag
     6061 cgatgcggca ggattactga cactggcagc ggtggcggtc atcggttcgc tgattatttt
     6121 cactctgcgt gtaaatcgta ctgttgcgca gaccgacgtg gcacatcatt aaataggtta
     6181 aggaacacga tgaacgcatt attaaccaat ccctttaaag aacgtttacg caagggcgaa
     6241 gtgcaaattg gtctgtggtt aagctcgact acttcttata tggcagaaat tgccgctact
     6301 tccggttatg actggttgct gattgacggg gagcacgcgc caaacaccat tcaggatctt
     6361 tatcaccagc tacaggcggt agcgccctat gccagccaac ccgtgatccg tccggtggaa
     6421 ggcagtaaat cgctgattaa acaagttctg gatattggcg cgcaaactct attgatcccg
     6481 atggtcgata ctgccgatca ggctcgtcag gtgtcgtctg ccacgcgcta tcctccctac
     6541 ggtgagcgtg gtgttggggc cagtgtggca cgggctgcgc gctggggacg cattgagaat
     6601 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc tggtggaaag taaaacggca
     6661 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacca
     6721 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     6781 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     6841 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     6901 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     6961 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     7021 gggcctgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     7081 cgagcacctg gtcacgatca atgccctgtt ccagcatgcg caaagcaatt ttaatggctt
     7141 gttcatgcat accttcctgc cagccaattt gatgcccttc ctgccgtagc cgttcagcaa
     7201 tagtcattaa tctctccttt tgtagtggtg aacgttcggc aatctcctca ataaaacggg
     7261 tgaaacggga ggtatcgccg cattgcagca gataattaaa cagtgtttgt agctggctgt
     7321 cattagtgaa gcctctaacc aaaagcgtgg tgatcctgtc gaccatgccg attaaatcgc
     7381 ggtcgcgaat atgcttttga atcagttcca gcagagctat acgccgatgt tgcatgatct
     7441 catcgtcagg tacgatggtg atatccacca acggaaaagc ttcggtgtac aactgccggg
     7501 caagttgcgg atcgtcaaac tcatccagcc agttaagtga gtacgggtag ggcgatgttt
     7561 cgccatgata aaacagcaat ggcaccacca gcggaactct gtcatagcct ttatccaggt
     7621 gacgctgcat ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag
     7681 agctttgatg ttcaatcacg cagtagatat agccgtcacc gtcgctggtt tccacggacc
     7741 acaaaacatc cgagtagtaa gcgcgtaaac ttttttcaat aaaactagtg ggttccaggc
     7801 gtaaggtttg caggttgcaa agcttgcgca gtggttctgg taaatgtatt tcgagaaaat
     7861 cccgtgcggt ttcgggtgtg aacataaagg ttttaaatac cgcatcatgc ggggaggagg
     7921 ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt
     7981 atcctaggcc tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt
     8041 cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc
     8101 acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagt agcgtaatcg
     8161 gatgttcgca gcgaagactt gtggacatct caatctgcca tttacaggtt tcgcagtcgg
     8221 tgaccaccag atctgcgccg ctttcttcta tctggcggaa cagtggtgcg ccgatggctt
     8281 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg
     8341 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
     8401 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
     8461 tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga
     8521 tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg
     8581 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc
     8641 ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga
     8701 ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
     8761 tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt
     8821 gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc
     8881 gacggaacgt gccgaaggag tatttcggta gcgtgcgacg atgatcgatt tttaacgccg
     8941 catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg
     9001 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc
     9061 gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg
     9121 ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg
     9181 cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac
     9241 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
     9301 cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
     9361 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
     9421 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag
     9481 cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt
     9541 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
     9601 gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa
     9661 atgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt
     9721 tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa
     9781 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
     9841 aagcatcagt gaacaaagta gtttttcatt caaccaacgc cacagtttgt catcggccag
     9901 gccgaagcag gcaggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
     9961 aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca cagcgcgaaa
    10021 ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac
    10081 cgaaagatcc agttcgtgca acgaagccgc cgcaagatgc gcctgaaaat ccatcaggcc
    10141 gcttatcccg accacacata ttttcttcgc gggcaacggc cagacgggga cttctggcga
    10201 acttagccag gtagagcgca gagtgccgag cggcgtaatc cgctggtgag ccagttctac
    10261 gctgccctgc aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc
    10321 gagcacgcgt tgtggcccga gaagagagta aggatgtgca ggtgcctgct gacgcaaaga
    10381 ttccagtcca ctgtgaatgt ctgccacggg ttgaccatct ggcagatggc tcagcaaatc
    10441 cagcgaaccg gatgagaaat gcaacgcgct ttgaccacgc gtgacaatgg cacagcgcag
    10501 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
    10561 agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc
    10621 gggtaaattc gctttcgcgc agtgcgtctc cccaggcgat gggttgcacg cctttccagc
    10681 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaacc
    10741 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggtg cccatcccca
    10801 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
    10861 gcacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt
    10921 cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
    10981 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg
    11041 ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag
    11101 cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt
    11161 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc
    11221 tcggatcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg
    11281 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg
    11341 ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg gtaccaatca
    11401 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga
    11461 tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
    11521 gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca
    11581 cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc
    11641 acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
    11701 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
    11761 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
    11821 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg
    11881 cctggaatga gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc
    11941 agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg
    12001 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
    12061 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc
    12121 gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt
    12181 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
    12241 catgaattgt ttgatttcgc gcatgttcgc tcataattcg aaagtgaaac gtgatttcat
    12301 gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
    12361 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat
    12421 caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt
    12481 aaaccagcgc cacacaaagc gcgcttacct gctgcggaga tcgatccgac ttatcgtcga
    12541 ttgcgctggc aaattttctt ggggatattc tttggctatg cggcttacta tttggttcgt
    12601 aagaactttg cgcttgctat gccttatctg gtcgagcagg gattctctcg cggtgattta
    12661 ggtttcgcgc tttcggggat ctcgatcgct tatggatttt cgaaattcat aatgggttcg
    12721 gtatcagatc gctcgaatcc gcgcgttttc ctccccgcag gtttgattct ggcggcggca
    12781 gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta
    12841 cttttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg
    12901 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac
    12961 aacgttggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
    13021 tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt
    13081 gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat
    13141 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa gcaaatcttc
    13201 atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt
    13261 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat
    13321 ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc
    13381 actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc
    13441 gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    13501 aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg
    13561 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    13621 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    13681 tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    13741 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta
    13801 ctacaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    13861 gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    13921 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    13981 agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga
    14041 cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg
    14101 tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga
    14161 aattaagtcg ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac
    14221 ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga
    14281 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tttatccaga
    14341 aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    14401 agtgctgaag aaatatggtt acaccggtaa agacgataaa gtttatttgc aatgttttga
    14461 tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca tggagctcaa
    14521 tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg
    14581 aagctgggtt aattacaact acgactggat gtttaagccg ggtgccatga aacaggtggc
    14641 ggaatatgca gatggtattg gtccggatta ccatatgttg attgaggaga catcgcagcc
    14701 gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca
    14761 tccttatacc gtgcggtcag ataaactgcc tgaatacact cctgatgtga atcagttata
    14821 tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc
    14881 agtaaaattt cttaataaag agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt
    14941 ctcatatcaa atttttccaa tcgccactaa ttgttccatg cacatttgta gtaaaaacag
    15001 tggactgcct tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaagttgg
    15061 ttcgagagga acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa
    15121 ggcgatgcct aatccctgtc gaataaaatt catcgctgta tccgcctgat taaattcaca
    15181 ttttatctgc tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg
    15241 aagggtaaaa gcggtgtcaa gctagtagtg gcaaaagaaa ttcattaata cgacacgttt
    15301 cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa acgacaggcg
    15361 atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg
    15421 cgactggggt gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga
    15481 gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc attctgtacg
    15541 ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaat aactggaacg
    15601 ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg
    15661 gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc
    15721 ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc
    15781 gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca
    15841 tatctatgtg aaaacagaag gtaaggcaga agctggtttt cttgacctgg agaaaagtcg
    15901 tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct
    15961 ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc
    16021 gttgtaggcc agataagaca cataagcgtt gtatctggca ctacaaccaa ttcacatctc
    16081 gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa
    16141 ggctaacggt tcggcaatcc agtcaacttg acctgcaacc aggcgtgtgc gacaggagcc
    16201 gcagtaacct tcgcgacact ggtactcaac cgccacattg tgggattcca gcgctgccag
    16261 aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg
    16321 ggccatcaga gctggaagtt actcaaatcg tcggtgtcca cttccgagtc aatctgcccg
    16381 accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc
    16441 caagtgttga tccacgggat cgggttggag cgcgtctgga acggcagatc caaaccgact
    16501 gcctgcatac ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga
    16561 ccaatcatcg aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc
    16621 tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga
    16681 tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct
    16741 tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca
    16801 aaggcaaagg aacaagcaaa gctgacgtag aagcggatcg cttccagcgc gttaacgctc
    16861 atcaggcaaa gatacagttt tttcttcagc tcgcgcaggc taacggttac ggttttaccg
    16921 ttaacggtgt gggtgccttc gcccagaagg tgccagtagc tggtcatttc gatcagttca
    16981 tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg
    17041 tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa
    17101 tgaatcgttt ctgagaacgc ccaggtttcg acccaggttt ccagttccgg aatagaaata
    17161 agcggcaata gcgccacgtt cggactacga ccctgaatgg aatccagcag cgtctgatat
    17221 ttcaggttgc tgataaaaat gtgtttttcg tgctccggca gcgcctggta atcgatacgg
    17281 tcgcgggaga cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc
    17341 ttttcgaaga tgtcatattt ttgctgatcg tagcgggcca cgttgaccgg ctgaccaaag
    17401 aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt
    17461 gtgtcctgtt gggagtttaa tgccggataa ggcattttac gccgcatcct gtgcctgatg
    17521 cgacgctggc gcgtcttatc aggcctacaa atccgagccg taggccggat aaggcgttca
    17581 cgctgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag ccatcgtcct
    17641 ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat
    17701 acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac tgctgcatcg
    17761 gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag atcgactgat
    17821 cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc ggcatttccc
    17881 acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacttgg cgcaaaatac
    17941 cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt
    18001 tagagatctg cgaagaggtc tcggacggca tcagggcaga aagcgtggag ttacgcaggc
    18061 cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgcagcggc tcattagcga
    18121 tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg taagtggttt
    18181 cgttaaacca cgggcactcg ccttgctctt tagccagctc attagacgct ttcagcaggt
    18241 aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc
    18301 gtttaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc agcgtacgac
    18361 gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc agcagcgcgt
    18421 caagtgcacg aaccgccaga attgccagct cttccagttc atccaggtta ttaattgcgc
    18481 ccaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt
    18541 tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc actggcgcga
    18601 tggccggatc aaacgggcta tgggtattac agtggtcaac gttctgaata tagatacgac
    18661 cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc acacgctgct
    18721 tgcggatgct gtcgtctttc tcatatttgg tatacagacg ttcaaactct tcctgatcgg
    18781 cgaagaacgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg atatcttcac
    18841 ctttcagcag acgggtatac atcagtttgt tgatttgtac cccgtagtcc atatgacgca
    18901 cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc acttccagat
    18961 gccacatcgg gtagaacagc gttgccgcac cgccgcgcac accgccctga gagcaggatt
    19021 tcaccgctgt ctggaaatgt ttgtagaacg gaatgcagcc ggtatggaac gcttcaccac
    19081 cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc ccggcacgct
    19141 gggaaacgta tttaacaatc gcgctggagg tggcgttgat ggaatccagg ctgtcaccgc
    19201 actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg gacatgattg
    19261 gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc acatattgca
    19321 ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata taaaggaact
    19381 gggcgctctc atagatttcg ccggtcacgc ggttctgtac cagatatttg ccttccagct
    19441 gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac gtgtccatct
    19501 gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg cccatctcga
    19561 ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag gcttttttac
    19621 gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca tcacgggaga
    19681 tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg ataccgtcat
    19741 aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc agtccttctg
    19801 ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg ctaccgtcgc
    19861 gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga attctttccg
    19921 caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact atatataggg
    19981 ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa gtgcataact
    20041 ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc
    20101 cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa
    20161 cggcagaata ggttggataa attgatatag atggtttatt aatctttttg ctaatgattg
    20221 caaaaattga tctaagcacg agtcatcaca taatctgatg tatcagttgt ggtcaatata
    20281 taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg ttattgttaa
    20341 atgcgaagcc attttgttaa tttaccttat attataaaaa aattattcat tactttaatt
    20401 tagtttaaat ataactggta tcccttaagg ttattaactt gaattatgac gcactgatat
    20461 tattcatcaa ataataacaa aatagccatt gcatcgggtt ggacaattta caaaagaaag
    20521 ataacccata ttgtataatg cgtttaaata tgccgtctta tataaaatga tggtcagatt
    20581 aatatgcgga ttatctttct acg
//
LOCUS       DADLTD010000058        20399 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLTD010000058 DADLTD010000000
VERSION     DADLTD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.058"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            127..942
                     /locus_tag="JN701_001686"
     CDS             127..942
                     /locus_tag="JN701_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAY7364163.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1070..1651
                     /gene="sodB"
                     /locus_tag="JN701_001687"
     CDS             1070..1651
                     /gene="sodB"
                     /locus_tag="JN701_001687"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY7364164.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1797..2966)
                     /locus_tag="JN701_001688"
     CDS             complement(1797..2966)
                     /locus_tag="JN701_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7364165.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3132..3221)
                     /gene="ynhF"
                     /locus_tag="JN701_001689"
     CDS             complement(3132..3221)
                     /gene="ynhF"
                     /locus_tag="JN701_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY7364166.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3520..4545
                     /gene="purR"
                     /locus_tag="JN701_001690"
     CDS             3520..4545
                     /gene="purR"
                     /locus_tag="JN701_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY7364167.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4542..5474)
                     /locus_tag="JN701_001691"
     CDS             complement(4542..5474)
                     /locus_tag="JN701_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7364168.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5587..6798
                     /locus_tag="JN701_001692"
     CDS             5587..6798
                     /locus_tag="JN701_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY7364169.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQTDLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7089..8237
                     /gene="cfa"
                     /locus_tag="JN701_001693"
     CDS             7089..8237
                     /gene="cfa"
                     /locus_tag="JN701_001693"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY7364170.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8277..8918)
                     /gene="ribE"
                     /locus_tag="JN701_001694"
     CDS             complement(8277..8918)
                     /gene="ribE"
                     /locus_tag="JN701_001694"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY7364171.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9133..10506
                     /gene="mdtK"
                     /locus_tag="JN701_001695"
     CDS             9133..10506
                     /gene="mdtK"
                     /locus_tag="JN701_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY7364172.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10547..11803)
                     /locus_tag="JN701_001696"
     CDS             complement(10547..11803)
                     /locus_tag="JN701_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364173.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12111..12187
                     /locus_tag="JN701_001697"
     tRNA            12111..12187
                     /locus_tag="JN701_001697"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12145..12147,aa:Val,seq:gac)
     gene            12192..12268
                     /locus_tag="JN701_001698"
     tRNA            12192..12268
                     /locus_tag="JN701_001698"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12226..12228,aa:Val,seq:gac)
     gene            12376..12681
                     /locus_tag="JN701_001699"
     CDS             12376..12681
                     /locus_tag="JN701_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY7364174.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12807..14411
                     /locus_tag="JN701_001700"
     CDS             12807..14411
                     /locus_tag="JN701_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY7364175.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFVQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14423..15235)
                     /locus_tag="JN701_001701"
     CDS             complement(14423..15235)
                     /locus_tag="JN701_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364176.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFRRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIAIAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGSGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15239..16024)
                     /locus_tag="JN701_001702"
     CDS             complement(15239..16024)
                     /locus_tag="JN701_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY7364177.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16021..16689)
                     /locus_tag="JN701_001703"
     CDS             complement(16021..16689)
                     /locus_tag="JN701_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7364178.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16753..17391)
                     /locus_tag="JN701_001704"
     CDS             complement(16753..17391)
                     /locus_tag="JN701_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY7364179.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAELYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17404..19506)
                     /locus_tag="JN701_001705"
     CDS             complement(17404..19506)
                     /locus_tag="JN701_001705"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAY7364180.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKCVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGSPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(19476..20153)
                     /locus_tag="JN701_001706"
     CDS             complement(19476..20153)
                     /locus_tag="JN701_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY7364181.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSFLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITVRILQRKKSWL
                     TVGQVIY"
BASE COUNT         5110 a         5307 c         4761 g         5221 t
ORIGIN      
        1 tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat
       61 tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga
      121 cgctcagtgg cgcagataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc
      181 ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact
      241 catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa
      301 acagcgaaaa aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa
      361 aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa
      421 acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt
      481 gtgaaagtca aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa
      541 aaagctacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg
      601 ggtggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa
      661 gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca
      721 gcgccaatcg aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga
      781 cgcggcacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg
      841 cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca gcgccactat
      901 gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta
      961 cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc
     1021 acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga
     1081 attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat
     1141 cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca atctgattaa
     1201 aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt
     1261 attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa
     1321 cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt
     1381 tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac
     1441 ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac
     1501 tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta
     1561 catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg
     1621 ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga
     1681 tgcgcttcgt ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa
     1741 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
     1801 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
     1861 gccagtcccg cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa
     1921 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
     1981 actgatgaag acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc
     2041 ggtacgaccg caaaggctgc tgcgccccac accaccatgc taatagctgc gccgaactca
     2101 ttgcgggcca ggaacgggat tgccagcata atcaccatca acaacaacaa aaagcctttc
     2161 aacgtgccgt taactgaacg atctgccagt ttgccgccgg gatagttacc gatagagaat
     2221 ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt
     2281 tgcagtaccg gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc
     2341 gtcagcaatg cagacaacac ctgcggacgc attaataccg ccagctcttt tttcacttca
     2401 ggtcgtgccc ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
     2461 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
     2521 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
     2581 ctggcctgtt tatgttttgg ctccacgctt gcggccacga ctgaacccaa accaaaaaat
     2641 gctccgtgat tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg
     2701 atggcggaaa gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
     2761 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca
     2821 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
     2881 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc
     2941 gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat
     3001 attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag
     3061 aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc
     3121 tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ccacgatcaa accgaggacg
     3181 ataatcgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta
     3241 ttaccacaca aatagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc
     3301 atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc caaaattcca
     3361 cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc
     3421 ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt taccttgcga
     3481 ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta
     3541 gcgaaacgag caaacgtttc cactacaact gtgtcacacg taatcaacaa aacacgtttc
     3601 gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct
     3661 agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc
     3721 agcagcgaag cggcctattt tgccgagatc attgaagcgg ttgaaaaaaa ttgcttccag
     3781 aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat
     3841 ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca
     3901 gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg
     3961 ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga aggcggctac
     4021 atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg
     4081 ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagcg
     4141 atgatcaagg tgccggaaag ctggattgtt cagggtgact ttgaacccga atctggttat
     4201 cgcgccatgc agcaaatact gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc
     4261 gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggtctgcg cgtcccgcag
     4321 gatgtttcgc tgatcggtta tgataacgtg cgcaacgccc gctattttac gccggcgctg
     4381 accacgatcc accagccaaa agattcgctg ggtgaaacag cgttcaacat gctgttggat
     4441 cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg cttgattgaa
     4501 cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga
     4561 gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc gagatactca
     4621 agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa
     4681 tccgggaagg ggttttccaa ttcaagtgct acccacttcc cctcattaag ccacggtttg
     4741 gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat
     4801 tcccagtcag gcacgacaac tcttttttgg ttatccagta accaggtaat acgtttaggt
     4861 agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg
     4921 aatgggccat ccatcaacgc cagcgggtgg tggctggcaa caacgcaact ccagcttagc
     4981 atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc
     5041 gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag
     5101 acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt
     5161 tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc gttcgctatc
     5221 tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc ttctttgaga
     5281 aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa gagcggcacc
     5341 gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac gcgatgcagc
     5401 tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa
     5461 tattctgacc acatagtcag cctgcaaaat ttttgaaatc agtcatcaaa tattaccgtt
     5521 tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga
     5581 atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt
     5641 tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacagaccga cctgcaaacg
     5701 cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag
     5761 cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg
     5821 acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc tacgctgctg
     5881 gtattgcgtt ttgtacaggc tgtgggtgtc tgcgccgcgg cggttatctg gcaagcgtta
     5941 gtgacggatt attatccttc acagaaagtt aaccgaattt ttgcgaccat catgccgctg
     6001 gtgggtctat ctccggccct ggctcctctg ttaggaagct ggctgctggt ccatttttcc
     6061 tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct gcctattttc
     6121 tggctcaaac ccacgacgaa ggccggtaac aatagtcagg atggtctgac ctttaccgac
     6181 ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcggcctg ttcagccagt
     6241 ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca
     6301 gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt
     6361 cgcgccgcgc tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt
     6421 gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa
     6481 atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc tattgttgtc
     6541 gcccaggcgc tgcgtccctt cccacacgca actggtcgcg ccgcagcgtt gcagaacact
     6601 ctacaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat tagtatcagc
     6661 acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt
     6721 tacatgatgc aacgttgtga agaagctggc tgccagaatc atggcaatgc cgaagtcgct
     6781 catagcgaat cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt
     6841 gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata
     6901 ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat ttcggcaagg
     6961 gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt
     7021 tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg
     7081 gagaaactat gagttcatcg tgtatagaag aagtcagcgt accggatgac aactggtacc
     7141 gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg
     7201 atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaagaaggc tctttggggt
     7261 taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca
     7321 aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta
     7381 ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaaag
     7441 agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt
     7501 cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa
     7561 tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct
     7621 ggggcggact ggcacactac atggcatcta attatgacgt aagcatggtg ggcgtcacca
     7681 tttctgccga acagcaaaaa atggctcagg aacgctgtga aggcctggat gtcaccattt
     7741 tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt
     7801 tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga
     7861 aaccggaagg catattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg
     7921 atccctggat taataaatat atttttccga acggttgcct gccctctgta cgccagattg
     7981 ctcagtccag cgaaccccac tttgtgatgg aagactggca taacttcggt gctgattacg
     8041 atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa attgcggata
     8101 actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc gcaggtgctt
     8161 tccgcgcccg tgatattcag ctctggcagg tcgtgttctc acgcggtgtt gaaaacggcc
     8221 ttcgagtcgc tcgctaaagg ctattctatc gccccctctc cgggggcgat ttcagatcag
     8281 gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta
     8341 tctaccactg cctgagtttg tggatcaatt tcaatgttga cgcgtgcgcc gagttttttc
     8401 ttcccaagag tcgtgcgttc cagagtttcc ggaattaaat ggacgcaaaa acgcgttggc
     8461 gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacaggata
     8521 tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt
     8581 aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt
     8641 tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca
     8701 agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt
     8761 tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc caggccgtcc
     8821 agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac
     8881 accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt
     8941 taagacattc tgttcagcac aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag
     9001 aatgactatt ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc
     9061 tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata
     9121 aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc
     9181 ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg
     9241 ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg
     9301 gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat
     9361 ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt
     9421 tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat ccgctccatg
     9481 gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg
     9541 ggcgcgccgg gctatctgtt cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa
     9601 accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacattcc ggtgaactat
     9661 atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct
     9721 actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc
     9781 tccatgcgcg atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa
     9841 cgactgattc aactcggttt accgattgcg ctggcactat tctttgaagt gacactgttt
     9901 gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt
     9961 gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc agcggtaact
    10021 atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg
    10081 accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac ggtttcactc
    10141 cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat
    10201 ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat tggcagtggg
    10261 attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac agcttactgg
    10321 gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg
    10381 gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc cattatgatg
    10441 atgttgcgta tgcggttcct gcaacgtctg ccgtcagtta tcattctgca acgagcatcc
    10501 cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac gcaggctgga
    10561 gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttgcac agagtttgcc
    10621 gactggcatc tgttatctca acgtgtatat caccggtaac ttcccctttc acattgatga
    10681 agtcaggtga gatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc
    10741 gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat
    10801 tcagattgat gttaccaaca ataaaagtga caccgtcacc ttttaccgta ctgccgtcga
    10861 gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagatct gcgccatagc
    10921 caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc
    10981 gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc
    11041 caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccga ccacccacat
    11101 tcacggacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc gtccccagac
    11161 gataaatgga tccattctca acaatggtgt cagagtcttc accaccgaga ttattcatgg
    11221 tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg ccgccctgat
    11281 taatctcaca attgctggct tccccacctg gatacaccaa caattcacca ccttcatcta
    11341 ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg agaatgattt
    11401 tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc aacaaaccgc
    11461 aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga
    11521 gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca atggcaccgt
    11581 cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc ccaccggttg
    11641 ccactttcaa ccagccagcc cccgtgacta cggtattttc ggctcttccg ccagcttcaa
    11701 caatcaactc gccttcagta agttgcgtcg cgccaatgac ctcgccggtc ttaacaattt
    11761 gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg
    11821 catctgtgtg cctttattgc tacctaaatg taaaggctac ggaggattta tccacgacag
    11881 atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat
    11941 cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat
    12001 ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc
    12061 accttcccac tctgccgcta atattcgtcc ctgttgtcac ctacaacgtt gcgttcatag
    12121 ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa
    12181 cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc
    12241 ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtcttct cctttttccc
    12301 tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc
    12361 agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa tggcccattt
    12421 ggcgacgcaa tggctgagca gcttaaaccg cttgctgagt cgattaatca ggaacctggt
    12481 tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg gatctatctt
    12541 tttactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg acttaaaaat
    12601 ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact gagtcaaatc
    12661 aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc ttttcatccc
    12721 tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa cactaaattc
    12781 gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta
    12841 cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct atttctatct
    12901 tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa
    12961 tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg tatctcgaat
    13021 ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc
    13081 acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat caatttttac
    13141 gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg
    13201 ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag gatttaccca
    13261 gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat gaagaagaag
    13321 caaccagaac gtattttccc agtccgtggt caggcctgat ggaagcaaag gtcgatgcgt
    13381 gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg gcagtggcta
    13441 ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttaac cgcgataacg
    13501 caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc gaagccgatt
    13561 tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca ttaaatgctg
    13621 agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata gtagaggaaa
    13681 tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg aatgtcgatt
    13741 cctttgttca agcctggttt gccgagcgca aacaacgcga cccatttgac tgggcagaaa
    13801 aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc tggcgttatg
    13861 tcattctgcg cctgcatgaa gccgtacagg aaattgttcc acatctgaat gaacacgacc
    13921 ataaacggtt cagtaaaggc cttgcccggg tttttatcga taattatgcg gcaatccctt
    13981 cagagtctat tcgtcggctg ctggccttac gtgaagcggg gatcattcat attctcgccc
    14041 tcggtgaaga ctacgaaatg gaaattaatg agtcgcgcac cgtcctgaaa acggaagaca
    14101 acagctactc gtttgacgtt tttattgatg cccgcggaca gcgtccgctt aaagtgaaag
    14161 atattccttt ccctgggcta cgcgagcaat tacagaaaac aggggatgaa atccctgatg
    14221 ttggcgaaga ttatacgtta cagcaacccg aagatattcg tggacgcgta gcgttcggcg
    14281 cgttgccctg gttgatgcac gaccagcctt tcgttcaggg acttacggca tgtgcagaaa
    14341 ttggtgaggc gatggctcgg gcggtcgtaa aacctgcatc ccgtgcacgt cggcgtcttt
    14401 cgtttgatta aaggtaaagc tatcagcact caacaaactg atgctgttca ttaagacgat
    14461 agcggactcc ggttcgaata ttgttttcac cttcaatggc gacggcgaag cgaactcttt
    14521 cgccgtcatg atatgccagt gcggcgcttc cccctgaccc gagaataatt gagtcgacca
    14581 cgcccgtgct cactaccgtt gaattttcac cgctggcaat gatgtgtaca ttgtcgccgc
    14641 tgttggcaat tctggcgttt gcgccaaagc tggcgatttg caccaggtca ccattactgg
    14701 cgacatgaca ccgttcaccc aatgtacaaa cgcgtactcg cattccggta ttcgcaatcc
    14761 gactactatc tccagcgctg cttatccgcg aagaattacc ggttacagca acccgtgcgc
    14821 gttcaccgga gctgccaatg tgactgttaa agccgacact tccaatccgc acgctataac
    14881 ccatactggc aatttgcgcg gcatacccag cgattgcgat tcgggcgtta tcctcacaac
    14941 agccgatgcg agcatgatcg tcgctgcggg caaactgact gcaaaatggt atctctccgg
    15001 ggtctgtggc cagtttttgc atagaggaaa cttcctgatg cgcgaagttt tcatccgcca
    15061 gccatttttt ccacgcatac tcaaccagac tctccgccca gtccgtatat ccttcctcaa
    15121 tcagtgcatg atgaatatcg gcataactac cgccgcgtcg gaaatggcgc aggaaccagc
    15181 gatacatcac cgctccgata cgccattctc gcaggtctgc tcgtgtaatg atcattcgtt
    15241 agtgccggtg atagccatcg accatgcttt taaaggtttc gtgtggcgta cgccccgtgg
    15301 tgcaaagata aagatgtccg aagataaaaa agaggcttat aaatgccaga gcaaaatgcg
    15361 cctgcaataa ccagtatctt acgccaggaa acacatctcc cacggcttgc ggatagagac
    15421 acaacagccc cgtcaatagt aacaacggca gcaatccata catgacacca acataggcga
    15481 cctgctgtaa gggattaaat ttagactgtg ttgttgccgg gaaaggatgt tcttccccct
    15541 gcataatgcc aaacaaataa aatcgcgttt gttttgccgc tcgttccagc cacccctgac
    15601 gacgaatgcg atagtggtga ccattatccc caacggcatt gatcagcaca aagccgagcc
    15661 agcatgccag taacaaaaat ccgcaaactt catgcaccgc aaccagactt ttaaccgcag
    15721 ttgcgcccac catcgcaaag tgatttatca gcccacttgc cagcaacaat acaaagagta
    15781 acgcattcga ccagtgccat aatctgaccg ctttgctgta aagataaacc ttctcaccat
    15841 gatcggtggc tgatttcttc acgccacgag cacgaagcaa ggcgtgcagc cccagcacca
    15901 accacatccc aaccagcaat actccggcaa tgatcaacca caccggccaa aattcgggag
    15961 tgaactgtgg cacatagttc gctaactggc tctgaaactg ttcagcatgt tgcgacgggt
    16021 tcatacattt tcctttttaa tcaaatgttg accgaaccgg cgatacagat gcggtttccc
    16081 ggcaccaggt agctgatatt gatagtattt attatcctgc aaccacgcct gaatttctgg
    16141 gctatcttcg cgaccaaaga tcaatgcgtg ttccggacag gcgctgacgc aaatgggcgg
    16201 aaagcctttt gccagtcggg actcagcgca gaaatcacat ttatctgcca ctttagtgac
    16261 agggttaagg tagcgaacct ggtacggaca agcgccaatg caataactgc agccgatgca
    16321 ctgcgatttt tctacccgca cgatcccctg ctcatcacgc cacgatgccc ccgtcgggca
    16381 aacgtcgatg cacggagcat cttcgcagtg ctgacatgac tggcgaaaaa agtgatactg
    16441 cgtttcgttg tcgttatcgg tgactggaat gtgtgctatc gataaacggc ttccctgagc
    16501 ggggacgtgg ttagttttac ggcatgcgcg ggcgcaaata ttacagccat tgcaccgtga
    16561 ctcgtcatga atcatggcat accgaacttc cttttcttgc ctcgtgttcg ctaacagaga
    16621 agacgcagaa ccagtaaaaa agattactgt tcccatcccc agaacaaatt tgcgtcgagt
    16681 gaaggacatc tgattatccc ctaatgcagt ggctgcaact gtccacccta tccatgacat
    16741 ctccctgaaa tttcaggggt tctgaaactg ctctacctcg aaccattcgc ataagccata
    16801 agccttgcca taggtcataa atcgctcact aaacaagtac aactccgccg tcgatgactc
    16861 cacctgccgt atatcggcat attcaggtgc aacatccatc gcggcgatcg cggcttcaat
    16921 ctgcgcttcc tggaagtaat atggcgcttg catcagcatg gcgactttgt aaggacgagg
    16981 atatgtctgg cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt cctgctccac
    17041 aacttgcaaa gacatcgccg cgtagttttc actcatggcc tgcgttgagt aatagtaatc
    17101 atcctggctt cccgtcatac aagcgatatc agcgtagtct ccgttctgct taatgctttc
    17161 cagcaaaact gacagctcct cttctgcaac cgaatagggt ggcgagagaa acacggcgcg
    17221 cgccaccagc tgcccagccg cagaatgctg gcgaatgaac tctgccagga tttcagccgg
    17281 ttctggttcg gttaccgtat caacggggtg acgcatcccc tgtaaccatt gccgcttcgc
    17341 ctcgtcaact tcgctgactt tcgctaacgg caattcatcc cgatggttca tctcacccat
    17401 tccttacaca ggcagtagat tgtgtgctgc cagatcggcg gcaatatctt ccagccccag
    17461 acgctgcaat gtttcgcggg tgggactacc aagctgtggg tcccagccca tttctttgta
    17521 gaacatcgtg agcgaagcat gcatatcgtc acgatccatt ttgtcagtac cttcagtaaa
    17581 gaccgggatc tgcggatcct tgtcgaatac ccaggaacag ataagatcgt gttcattacg
    17641 catatctttg gtttgcataa gttttaccgt gtaggcacga tgcaacgtaa agatacgctc
    17701 tgcagctaaa tctaattttt cctgagtcat ctcttcgccg gtgatcgctt tgaagaattt
    17761 ggcttcaagc gccagatcgc cccggtaatt acggcttttc agcggcgaaa cggtcattgg
    17821 ccagacccag ttacacaggg tgacggcgtt atgcaaacag acccgcaaca gcgaccactt
    17881 agcatattta atttttgcgt cattgattgg cgtgtagttt ttggtttcat cgtaggcatc
    17941 ttcagaacca aaaagctctt tcgccacttc acgttgcagt ttcaatggca agccggaacc
    18001 aataaagttg atatgggtat gtgtcatgca gtcacggttg aacatacagt taacaatgga
    18061 accgacctgc gctgacgctt cattggcatg gtgaaccgga tagccaaacg gcgaccagag
    18121 tttatttttc gcgtagcccc agtactcttc acccaaattc cagcgttctg cgatggcata
    18181 tgaaccatca gccaggtgac tcagctcacc cacacgatgc gccagacggt agtaaaaatc
    18241 tttaatgaaa ttaacgtcac ccgcttccag ttgatcccag tgaatttccg catactcttc
    18301 agcaggcaga acacacttga acaccccttt gctgtaacaa taagtaaagt cgcgatgcaa
    18361 ctgcccgtag ttacaccata ggccgtagtc atcgaacaaa ttcagaccaa ccaagttacc
    18421 aatcacacgg ccatcgtctt tatcttcaaa atctttcgga ccgttcggga agatggtggt
    18481 atggacaaag ttggctacac aagtgttacc acctgtgctg ggcacgccaa actctttcac
    18541 ccgaggaata ttcatttggg tcatacaacg gatcgggcaa gagtggcagc cactcatttt
    18601 tactgtgtat ttctctgccg ccggacctaa gtcaaaaacg gatttatagg tacgaaagcc
    18661 gaccgtgttc tgattgcctg gcggaatttc ccccgtttca atcggaccac cttcagccgc
    18721 gccccaaaac agccctttac gtgctgtcca acgtgacttg ggatctgaat actctgccca
    18781 cgattgcggc gtacttggca cgacatggtt gttattcgca ccaataagtt cagtcatcat
    18841 gtaatcgttg agacgcttca tctcctgacg atcggcaatg ttgacccctt tcgtcccttc
    18901 caccgcaatc gctttcaggt ttttcgaacc cataattgcg ccagttcccg caccgccgct
    18961 gtggttacgg ctattcaaca tgccagagag aggaacaagg ttttccccag cctggccaat
    19021 agccgccaca caggtttccg gactggtcaa tcgacaaatt tcttccgtcg ttgcgcgcgt
    19081 cccttttccc cataagaaat cggctttttc caggctaact ttgtcatctt taatcttcag
    19141 ccataccggt gatttcgcct tcccttcgat aataatgacg tcgtatccag cgaatttcat
    19201 ttgcgctgca aaaaagccac ccatatgggc atcgacaact aaatttcctt tggtaaaagt
    19261 agaaagtgag gtgatattta cgcgagaact acagggggca ccagatccag ttaatgggcc
    19321 ggtagcaaag actaatttat tcgcttcatc gaaaggtttc gtgcctggcg gtacttcgtc
    19381 atacataatt ttgtagccga agcccatgcc accgacaaaa cttttaaact tactggaatc
    19441 ttcgagggta atatttcctg tcgtgagatt gactcttaat atattacctg tccaaccgtt
    19501 agccatgatt ttttcctttg caagattctc acagtaatat ctttccactc gataattttt
    19561 aacgcccctg ttgggcaggc gtttgcacat tcaccgcata acacacattt cgaggatttt
    19621 ttactttcgg tatttacggt ggccatcatc cacgggcatg ccgtagtaca ggcgctacag
    19681 ccaatacagc gtttatgatc gacggtaata cagccttctt tctgctgcca ggtaatcgca
    19741 ccaatcgggc agacgttcat gcattgcggc tctttgcatt gacggcaggt gtccgcggta
    19801 tagttgagat cgccatacag gccgccgcca gagccaaccc cgttgtcgcc aaagaaataa
    19861 ttgcgatgga ttttgatacg ggagaagaat gtccctactg agccatcgtt gaagttggta
    19921 caagagattt cacagcggtg gcaacccgtg catcgcgctc gctgggtcac cagcacccct
    19981 ttgggtgtat ttatcaaccc tactgtgccg ctatcaatat cttcctgttt gcaacccaaa
    20041 aatgacagta acgcaggggc aattgttaga ccagcaaggc cttttcctga tatacgcaag
    20101 aattctaatc gcgttaagcc aatatctaat agtggacgat caaccgggtt catttatttc
    20161 gcatcctctt cgcaaacaca gtgctgacaa ttcagcagcc tgatattaag cattcaatta
    20221 atacgctgct gactctgtcc ccaacaccag atattttccc taacccgata gtgtatgaga
    20281 aaagatttaa cgccgataaa ctaatccttt atggttagga gaaaaaataa tgtgattatt
    20341 ctgagccctt aacattgatc gttatcaatt aaaattacaa agcaagaaat tataagtga
//
LOCUS       DADLTD010000059        20025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLTD010000059 DADLTD010000000
VERSION     DADLTD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.059"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..309
                     /locus_tag="JN701_001707"
     CDS             <1..309
                     /locus_tag="JN701_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7364182.1"
                     /translation="IIVQWDANSNGIWDREPVKESDQIGFRLKEHVLETLRGATSCEG
                     KGWDKVTNPDAIIIDTFQVVRQDVSGFSPVLTVNMRAASKSEPQTVVNASYSVTGFNL
                     "
     gene            306..713
                     /locus_tag="JN701_001708"
     CDS             306..713
                     /locus_tag="JN701_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY7364183.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            698..1021
                     /locus_tag="JN701_001709"
     CDS             698..1021
                     /locus_tag="JN701_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7364184.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            1034..4402
                     /gene="recC"
                     /locus_tag="JN701_001710"
     CDS             1034..4402
                     /gene="recC"
                     /locus_tag="JN701_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY7364185.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            4578..7466
                     /gene="ptrA"
                     /locus_tag="JN701_001711"
     CDS             4578..7466
                     /gene="ptrA"
                     /locus_tag="JN701_001711"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY7364186.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFHYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            7459..11001
                     /gene="recB"
                     /locus_tag="JN701_001712"
     CDS             7459..11001
                     /gene="recB"
                     /locus_tag="JN701_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7364187.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRARIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQATQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSFVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            11001..12827
                     /locus_tag="JN701_001713"
     CDS             11001..12827
                     /locus_tag="JN701_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecBCD enzyme subunit RecD"
                     /protein_id="HAY7364188.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(12889..14220)
                     /gene="argA"
                     /locus_tag="JN701_001714"
     CDS             complement(12889..14220)
                     /gene="argA"
                     /locus_tag="JN701_001714"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY7364189.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            14452..15705
                     /locus_tag="JN701_001715"
     CDS             14452..15705
                     /locus_tag="JN701_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY7364190.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15964..16143
                     /locus_tag="JN701_001716"
     CDS             15964..16143
                     /locus_tag="JN701_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAY7364191.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFFMSEMSKKINSTN"
     gene            16140..16787
                     /locus_tag="JN701_001717"
     CDS             16140..16787
                     /locus_tag="JN701_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7364192.1"
                     /translation="MIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNAI
                     SRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNN
                     YENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEKI
                     KQKNIGDIYEQFLEKDFQLQRAIHKIERYLYATFKYDLILEEKLYLSIRIKVLMD"
     gene            16819..18399
                     /locus_tag="JN701_001718"
                     /pseudo
     CDS             16819..18399
                     /locus_tag="JN701_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031400.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            18399..19607
                     /locus_tag="JN701_001719"
     CDS             18399..19607
                     /locus_tag="JN701_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HAY7364193.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            19576..>20025
                     /locus_tag="JN701_001720"
     CDS             19576..>20025
                     /locus_tag="JN701_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAY7364194.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFIRSHHSSLAISCDLSVEIPVKSEADNL"
BASE COUNT         4966 a         4665 c         5341 g         5053 t
ORIGIN      
        1 atcattgtgc agtgggatgc gaacagtaac ggtatctggg atcgcgaacc ggtaaaagag
       61 tccgaccaga ttggatttcg tctgaaggag catgtgctgg aaacgctacg cggtgcgaca
      121 tcctgtgaag gtaagggctg ggataaagtc actaatccgg atgccatcat tatcgacact
      181 tttcaggtcg tacgtcagga tgtcagcggc ttctcgccgg tgttgacggt taatatgcgt
      241 gctgccagta agtctgaacc gcaaaccgtg gtgaatgcta gctatagcgt gacaggattc
      301 aacctgtgaa ccgcgaaaag ggtgtttcgt cactggctct ggtcctgatg ctgctggttt
      361 tgggtagttt gctattacaa ggaatgagcc aacaggatcg cagttttgcc tctcgcgtga
      421 gcatggaaag tcagtcattg cgccgccagg ccatcgttca gtcggcgctg gcgtggggaa
      481 aaatgcactc ctggcagacg cagccagcag ttcagtgctc gcagtacgct gaaaccgatg
      541 cccaggtttg tttgcgttta ctggcagata atgaagcctt attgattgcc ggttatgaag
      601 gcgtttcgtt gtggcgaaca ggcgaagtca ttgatggaaa cattgttttt tcgccaagcg
      661 gctggagcga tttttgtccg ctgaaagaga gggcgttatg tcagcttccc tgaagaatca
      721 acaaggcttt agcctgccgg aggtaatgtt ggcgatggtg ttgatggtga tgattgtcac
      781 tgcgttatcg ggtttccagc gaacattaat gaacagtctt gccagcagaa accagtacca
      841 acagctctgg cggcatggct ggcagcaaac gcaactgcgc gcgatttcgc caccagctaa
      901 ctggcaggtc aaccgattgc agacatcgca ggcgggatgt gtcagcatca gcgttacgct
      961 agtttcaccc gggggcagag aaggcgagat gacccgcctg cattgcccga atcgtcagta
     1021 gtcaggagcc gctatgttaa gggtctacca ttccaatcgt ctggacgtgc tggaagcgtt
     1081 gatggagttt attgtcgaac gcgaacggct ggacgatcct ttcgaaccag agatgattct
     1141 ggtgcaaagt accggtatgg cacagtggct gcaaatgacc ctgtcgcaaa agtttggtat
     1201 tgcggcaaac attgattttc cgctgcctgc gagctttatc tgggatatgt tcgtccgggt
     1261 gttaccggag atccccaaag agagcgcctt taacaaacag agcatgagct ggaaactgat
     1321 gactctgctg ccgcaactgt tggagcacga agactttacc ctgttgcggc attatctgac
     1381 tgacgatagc gacaagcgaa aactgttcca gctttcctca aaagcggcgg acctgtttga
     1441 ccagtatctg gtctatcgcc cggagtggct ggcacaatgg gaaacaggac atttggttga
     1501 agggctggga gaagcacagg cctggcaagc gccgttgtgg aaggcgctgg tggaatatac
     1561 ccatgaactc ggacaaccgc gctggcaccg cgcgaatctc tatcagcgct ttatcgaaac
     1621 gctggagtcc gcgacgacct gcccgccggg gttaccttcg cgcgtcttta tatgcggtat
     1681 ttccgcgtta ccgcctgttt atctccaggc gctccaggcg ctgggtaaac atattgaaat
     1741 ccatctcctg tttaccaacc cctgccgtta ttactggggc gatattaaag atcctgctta
     1801 tctggcgaaa ctgctgactc gtcagcgtcg acacagtttt gaagatcgcg aattgccgct
     1861 atttcgcgat agcgaaaatg ccggacagct ctttaacagc gatggtgaac aggatgtcgg
     1921 caacccgctg ctggcctcat gggggaagct tgggcgcgac tacatttatc tcctttctga
     1981 cctggagagc agccaggagc tggacgcctt tgtcgatgtc acgccagata acctgctgca
     2041 taacattcag tctgacattc tggaactgga aaaccgtgcc gttgctggtg tgaacatcga
     2101 agagttttcc cgtagcgata acaaacgccc gcttgatcca ctggatagca gtatcacctt
     2161 ccacgtttgc catagcccgc agcgtgaagt tgaagttttg cacgatcgcc tgctggcgat
     2221 gctggaagaa gacccgacac ttactccgcg cgacatcatc gtgatggtgg ctgatatcga
     2281 cagctacagt ccgtttattc aggctgtgtt tggtagcgca cctgcggatc gttacctgcc
     2341 ttacgccatt tccgaccgtc gggcgcggca gtcgcatccg gtactggaag cgtttatcag
     2401 cctgttatcg ttgccagaca gccgctttgt gtcggaagac gtgctggcat tactggatgt
     2461 gccggtgctg gcggcgcggt ttgacatcac cgaagaaggg ctgcgttatt tacgcctgtg
     2521 ggtcaacgaa tccggcattc gttgggggat agatgacgac aacgttcgcg agctggaact
     2581 ccccgccacc ggacaacaca cctggcgatt tggcctgacg cgtatgttgc tgggctacgc
     2641 gatggagagc gcgcagggcg agtggcaatc ggttctacct tatgatgagt cgagcggctt
     2701 aattgcagaa ctggtggggc atctggcttc actgctaatg cagctaaaca tctggcgtcg
     2761 cgggctggca caggagcgtc cgctagaaga gtggttgccg gtttgtcgcg atatgctcaa
     2821 cgccttcttc ctgccggatg cggaaaccga agcggcgatg acgctgatcg aacaacaatg
     2881 gcaggcgatt atcgccgaag gtttaggtgc gcagtatggc gacgcggtgc cgctgtcact
     2941 attgcgtgat gaactggcac agcgtctgga tcaagaacgt atcagccagc gttttctcgc
     3001 cggaccggtt aacatttgta ctctgatgcc aatgcgttca attccgttca aagtggtttg
     3061 cctgctggga atgaacgacg gcgtttatcc acgtcagctt gcgccattgg gctttgacct
     3121 gatgagccag aaaccgaagc gtggcgaccg tagccgtcgc gatgacgacc gctacctgtt
     3181 cctggaagcg ttaatttccg cgcagcaaaa actctatatc agttatatcg ggctttccat
     3241 tcaggataac agtgaacgtt tcccgtcggt actggtgcag gaactgatcg actacatcgg
     3301 gcaaagtcat tatctaccgg gcgatgaagc gctcaactgt gatgaaagcg aggcaagggt
     3361 aaaagcgcat cttacctgcc tccatacccg gatgccgttt gatccacaaa actaccagcc
     3421 aggcgaacga caaagctatg ctcgtgaatg gctacctgcg gccagccagg ctggtaaagc
     3481 acattctgaa tttgttcagc cgctgccgtt taccttaccg gaaaccgtgc cgctggaaac
     3541 gctacaacga ttctgggcac atccggtgcg ggcatttttc cagatgcgtt tgcaggtgaa
     3601 cttccgtacc gaagacagcg aaatccccga caccgagcca tttattctgg aagggcttag
     3661 ccgttatcaa atcaatcagc agttattgaa tgcactggtt gagcaggatg atgccgaacg
     3721 cttgttccgt cgcttccggg cggcagggga tttaccgtat ggcgcttttg gtgaaatttt
     3781 ctgggaaact cagtgccagg agatgcagca gcttgccgac agagtcattg cctgtcgcca
     3841 gccggggcag agtatggaaa ttgatctcgc ctgcaacggt gtgcagataa ctggctggtt
     3901 gccgcaggtg cagccggatg gcctgttgcg ctggcgtccc tctttattaa gtgtggcgca
     3961 gggaatgcaa ctttggctgg aacaccttgt ctactgtgcc agcggtggta atggtgaaag
     4021 tcgccttttt ctacgcaaag acggcgagtg gcgttttccg ccgcttgcag ccgaacaggc
     4081 tttgcattac ctctcacaac tgattgaggg gtatcgtgaa gggatgtccg cgccattgct
     4141 ggtgttacct gaaagtggcg gcgcgtggct aaaaacctgt tatgacgcgc aaaacgatgc
     4201 catgctggat gacgattcca cgttgcaaaa agcccgtacg aaattccttc aggcttacga
     4261 aggcaacatg atggtgcgtg gcgaaggtga taatatctgg tatcaaaggc tctggcggca
     4321 attaacacca gagacaatgg aggccatcgt tgaacagtcg caacgtttcc tgttaccgct
     4381 gtttcgcttt aatcagtcat gagtgctgta taaaaattgc gcaatctatc cgcttacttt
     4441 atgatgcgca ccagtcacgg actgatggtt atataaacat aggctgactc gtgcagcaca
     4501 agattaaatt ctggcagatg atttgcgtta acgtgttgaa tctggacaga aaattaagtt
     4561 gattatgagg tccgtgaatg ccccgcagca cttggttcaa agcattattg ttgttagttg
     4621 ccctttgggc acccttaagt caggcagaaa cgggatggca gccgattcag gaaaccatcc
     4681 gtaaaagtga taaagataac cgccagtatc aggctatacg tctggataac ggtatggtgg
     4741 tcttgctggt ttctgatccg caggcagtta aatcgctctc ggcgctggtg gtgcccgttg
     4801 ggtcgctgga agatcccgag gcgtaccagg ggctggcaca ttaccttgaa catatgagtc
     4861 tgatggggtc gaaaaagtac ccgcaggctg acagtctggc cgaatatctc aaaatgcacg
     4921 gcggtagtca caatgccagc actgcgccgt atcgcacggc tttctatctg gaagttgaga
     4981 acgacgcctt gcctggtgcg gtagaccgcc tggccgatgc tattgctgaa cctttgctcg
     5041 acaagaaata tgccgaacgt gagcgtaatg cggtgaacgc tgaattaacc atggcgcgta
     5101 cgcgtgacgg gatgcgcatg gcacaggtca gcgcagaaac cattaacccg gcacaccccg
     5161 gttcaaagtt ttctggtggt aacctcgaaa ctttaagcga taaacctggt aatccggtgc
     5221 agcaggcgct gaaagatttc cacgagaagt actattccgc caatctgatg aaggcggtta
     5281 tttacagcaa taaaccgctg ccggagttgg caaaaatggc ggcggacacc tttggtcgcg
     5341 tgccgaacaa agagagcaaa aaaccggaaa tcaccgtgcc ggtagtcacc gacgcgcaaa
     5401 agggcattat cattcattac gtcccggcac tgccgcgtaa agttctgcgc gttgagtttc
     5461 gcatcgataa caactcagcg aagttccgta gtaaaacgga tgaattgatt acctatctga
     5521 ttggtaatcg cagcccaggt acactttccg actggctgca aaagcaggga ttagttgagg
     5581 gcattagcgc caactccgat cctatcgtca acggcaacag cggcgtatta gcgatctctg
     5641 cgtctttaac cgataaaggc ctggctaatc gcgatcaggt tgtggcggca atttttagct
     5701 atctcaatct gttacgtgaa aaaggcattg ataaacaata cttcgatgaa ctggcgaatg
     5761 tgctggatat cgacttccat tatccgtcga tcacccgtga tatggattac gtcgaatggc
     5821 tggcagatac catgattcgc gttccggttg agcatacgct ggatgcagtc aatattgccg
     5881 atcggtacga tgctaaagca gtaaaagaac gtctggcgat gatgacgccg cagaatgcgc
     5941 gtatctggta tatcagcccg aaagagccgc acaataaaac ggcttatttt gtcgatgcgc
     6001 cgtatcaggt cgataaaatc agcgaacaaa ctttcgctga ctggcagcaa aaagctgcca
     6061 atattgcgct ctccttaccg gagcttaacc cctatattcc tgacgatttc tcgctgatta
     6121 agtcagagaa gaaatatgac catccagagc tgattgttga tgagtcgaat ctgcgcgtgg
     6181 tgtatgcgcc aagccgttat ttttccagcg aacccaaagc tgatgtcagc ctgattttgc
     6241 gtaatccgaa agccatggac agcgcccgca atcaggtgat gtttgcgctc aatgattatc
     6301 tcgcagggct ggcgcttgat cagttaagca accaggcgtc ggttggtggc ataagttttt
     6361 ccaccaacgc taacaacggc cttatggtta atgctaatgg ttacacccag cgtctgccgc
     6421 agctgttcca ggcattgctc gaggggtact ttagctatac cgctacggaa gatcagcttg
     6481 agcaggcgaa gtcttggtat aaccagatga tggattccgc agaaaagggt aaagcgtttg
     6541 agcaggcgat tatgcccgcg cagatgctct cgcaagtgcc gtacttctcg cgagatgaac
     6601 ggcgtaaaat tttgccctcc attacgttga aagaggtgct ggcctatcgc gacgccttaa
     6661 aatcaggggc tcgaccagag tttatggtta tcggcaacat gaccgaggcc caggcaacaa
     6721 cgcaggcacg cgatgtgcaa aaacagttgg gcgctgatgg ttcagagtgg tgtcgaaaca
     6781 aagatgtagt ggtcgataaa aaacaatccg tcatctttga aaaagccggt aacagcaccg
     6841 actccgcact ggcagcggta tttgtaccga ctggctacga tgaatacacc agctcagcct
     6901 atagctctct gttggggcag atcgtacagc cgtggttcta caatcagttg cgtaccgaag
     6961 aacaattggg ctatgccgtg tttgcgtttc caatgagcgt ggggcgtcag tggggcatgg
     7021 gcttcctttt gcaaagcaat gataaacagc cttcattctt gtgggagcgt tacaaggcgt
     7081 ttttcccaac cacagaggca aaattgcgag cgatgaagcc agatgagttt gcgcaaatcc
     7141 agcaggcggt aattacccag atgctgcagg caccgcaaac gctcggcgaa gaagcatcga
     7201 agttaagtaa agatttcgat cgcggcaata tgcgcttcga ttcgcgtgat aaaatcgtgg
     7261 cccagataaa actgctgacg ccgcaaaaac ttgctgattt cttccatcag gcggtggtcg
     7321 agccgcaagg catggctatt ctgtcgcaga tttccggcag ccagaacggg aaagccgaat
     7381 atgtacaccc tgaaggctgg aaagtgtggg agaacgtcag cgcgttgcag caaacaatgc
     7441 ccctgatgag tgaaaagaat gagtgatgtc gccgagacac tagatccttt gcgcttgccc
     7501 ttacagggtg agcgcctgat tgaagcctct gccggcacag gcaaaacctt tacgattgcg
     7561 gcgctctatt tgcgcctgtt acttggacta ggcggttccg ccgcctttcc ccgcccgctg
     7621 accgttgaag aactgctggt ggtcaccttt accgaggctg ccacggcaga attgcgtgct
     7681 cgtatccgta gcaatatcca tgagttgcgc atcgcctgtc tgcgtgaaac taccgacaat
     7741 ccactgtacg aacgtctgct ggaagagatc gacgataaag cgcaagccac gcagtggttg
     7801 ttgttagccg aacggcagat ggatgaagcg gcagtcttta ctattcacgg tttttgccag
     7861 cgcatgctca acctgaatgc ctttgaatcc ggcatgctgt ttgagcagca gctgattgaa
     7921 gatgagtctc tgctacgtta ccaggcctgc gccgatttct ggcgtcgcca ctgctacccg
     7981 ctgccgcgtg aaatagccca ggtcgtcttt gaaacctgga aagggccgca ggcgttgctg
     8041 cgcgatatta atcgttatct gcaaggcgaa gcgccggtta tcaaagcacc gccgcccgat
     8101 gatgaaacgc tggcttcccg ccacgcgcaa attgtggcgc gtattgctac ggtaaaacag
     8161 cagtggcgcg acgcagtggg tgaactggat gcgctgatcg aatcttctgg tattgatcga
     8221 cgcaagttta accgtagcaa tcaggctaaa tggatcgaca agatcagcgc ctgggcagaa
     8281 gaagagacca acagttatca gttgccggag tcgctggaaa aattctccca gcgtttctta
     8341 gaagatcgca cgaaagctgg gggggaaacc ccgcgacatc cactgtttga ggcgatcgat
     8401 caactgcttg cagaaccatt gtcgatccgc gatctggtga tcacccgcgc attggctgag
     8461 atccgcgaaa cagtagcgcg tgaaaaacgc cgccgtggcg aattgggttt tgatgacatg
     8521 ttaagtcggc tcgattccgc gctgcgtagc gaaagcggtg aggtgttggc agcggcgatc
     8581 cgtacgcgat tcccggtggc aatgatcgat gaatttcagg ataccgaccc ccagcagtac
     8641 cgaatttttc gccgtatctg gcaccatcag ccggaaaccg cattgttgct gattggcgac
     8701 ccgaagcagg ccatatatgc attccggggt gcggatatct tcacttatat gaaggcgcgt
     8761 agtgaagttc acgcccacta cactttagac accaactggc gctccgcacc tggaatggtg
     8821 aacagtgtga acaagctttt cagccagact gatgacgcgt tcatgtttcg cgaaataccg
     8881 tttattccag tgaaatcagc cgggaaaaat caggcgttac gttttgtatt taaaggtgaa
     8941 acgcagcctg cgatgaaaat gtggctgatg gaaggcgaaa gctgcggcgt tggcgactat
     9001 caaagtacca tggcgcaggt atgtgctgcg caaatccgcg actggctaca agccggacag
     9061 cggggcgaag cgttgctgat gaacggcgac gacgcgcgtc cggtgcgtgc ttcggacatc
     9121 agtgtgctgg tgcgcagccg ccaggaggcc gcccaggtgc gcgatgcctt aacgttgctg
     9181 gaaatccctt ccgtttacct ttcgaaccgc gacagtgttt ttgaaactct ggaagcgcag
     9241 gaaatgcttt ggttattgca ggcggtgatg acgcccgaac gtgagaacac cctgcgtagt
     9301 gcgctggcaa cgtcaatgat ggggctgaac gcgctggata tcgaaacgct gaataatgac
     9361 gaacatgcgt gggatgcggt agttgaagag ttcgatggtt atcggcaaat ctggcgcaaa
     9421 cgtggcgtta tgccgatgct gcgggcgctg atgtcggcgc gtaacattgc agaaaacttg
     9481 ctggcaacgg caggcggtga gcggcgtctt accgatatct tgcatatcag cgaactgcta
     9541 caagaagccg gaacgcagct ggaaagtgaa catgcgctgg tacgctggtt atcgcaacat
     9601 atcctcgagc cagacagtaa tgcctccagc caacaaatgc gcctcgaaag tgataaacat
     9661 ctggtgcaga ttgtcacgat ccataaatcg aaagggctgg aatatccgct ggtctggttg
     9721 ccatttatta ccaatttccg cgtccaggat caggcgtttt atcacgatcg ccactcgttt
     9781 gaggcagttt tggatcttaa tgctgcgcca aaaagcgtcg acctcgcgga ggtcgaacgt
     9841 ctggcggaag atctgcgttt gctttacgtg gcgctgacgc gttcggtttg gcattgcagt
     9901 ctcggcgttg caccgctggt gcgccgtcgt ggcgataaaa aaggtgacac cgacgtccac
     9961 caaagtgcgc tcgggcgttt gctgcaaaaa ggggaaccgc aagatgcggc agggcttcgc
    10021 acctgtattg aagcgttatg cgatgatgat attgcctggc aaacggcaca aactggtgat
    10081 aaccagccct ggcaggttaa tgatgcttta actgcagaac tgaatgcgag gacactacaa
    10141 cgattgcccg gcgataactg gcgtgtcacc agctactccg gcttgcagca gcgtggtcac
    10201 ggtatcgctc aggatctgat gccacggctg gatgttgatg ccgcaggcgt ggtcagcttc
    10261 gttgaagaac cgacgttaac accgcatcag ttcccgcgcg gtgcgtcacc ggggacattc
    10321 ttgcacagtt tgtttgaaga cctcgatttt acccagccgg ttgacccgaa ctgggtacag
    10381 gaaaaactgg aactcggcgg ctttgaatcg cagtgggaac cggtgttgac cgagtggatc
    10441 acggctgtcc tccaggcacc tctcaatgaa aagggggtta gcctgagtca gctttccgac
    10501 cgcgataaac aggtggagat ggagttttac ctgccgatta gcgaaccgct catcgccagc
    10561 cagcttgatg cactaattcg ccagtttgat ccgctatccg ctggctgccc gccgctggag
    10621 ttcatgcagg tacgcggcat gttaaaaggc tttatcgacc tagtattccg ccacgaaggg
    10681 cgttattacc tgctcgacta taaatccaac tggctgggtg aagacagttc ggcttacacc
    10741 caacaggcta tggcagcagc gatgcaggca caccgctatg atctgcaata tcagctttat
    10801 accctggcgc tgcatcgtta tctgcgccat cgcattgctg attacgacta tgatcgtcac
    10861 tttggcggcg ttatctatct gttcctgcgt ggcgttgata aagaacatcc gcaacaaggg
    10921 atttacacaa cccgacccaa cgccgggttg attgccctga tggatgagat gtttgccggt
    10981 atgaccctgg aggaggcgta atgaaattgc aaaagcaatt actggaagct gtggagcaca
    11041 aacagctacg cccgctggat gtgcaatttg ccctgaccgt ggcgggagat gaacatcctg
    11101 ccgtcaccct cgcggcggca ctgttaagtc atgatgccgg agagggacac gtctgtttgc
    11161 cgctttcacg actggaaaat aacgaggcgt cgcatccgct gttggcgacc tgtgtcagtg
    11221 aaatcggtga gctacaaaat tgggaagaat gcttgctggc ttctcaagcg gtcagcaggg
    11281 gagatgaacc cacgccgatg atcctctgtg gcgatcgtct ttatttgaat cgcatgtggt
    11341 gtaacgagcg gacggtagcg cgcttcttca atgaggtgaa tcatgccatt gaggttgatg
    11401 aagctctact ggcgcaaacc ctggacaaac tttttccagt aagcgatgaa attaactggc
    11461 aaaaagttgc agcggcagtg gcgctgacgc ggcggatctc ggtgatttcc ggcggcccag
    11521 gcaccggtaa aacgaccacc gtggcgaagt tgctggcagc gttaattcaa atggccgacg
    11581 gcgaacgctg ccgtatccgt ctggctgcac caacgggtaa agctgccgcg cgcttaaccg
    11641 aatctctcgg caaggctttg cgacagttac cgctgaccga tgaacaaaag aaacgcattc
    11701 cggaagatgc cagcactttg caccgattgc tgggcgcgca gccgggtagc cagcgtttac
    11761 gtcatcatgc cggtaacccg ctgcatcttg atgtgctggt ggtagatgaa gcgtcaatga
    11821 tcgatctgcc tatgatgtcg agactgatcg acgccttgcc cgatcatgcg cgagtgatct
    11881 ttctcggcga tcgtgatcaa ctggcctcgg ttgaggctgg ggctgtgctg ggcgatatct
    11941 gcgcttatgc caacgcgggc tttaccgccg agcgtgccag gcagctaagc cgcctgacgg
    12001 ggactcacgt tccggcagga accggcacag aagcggcatc tttgcgcgac agtctctgcc
    12061 tgctgcaaaa aagctatcgt ttcggcagcg attctggcat tggtcagtta gctgcggcga
    12121 tcaaccgtgg tgataaaacg gcagtgaaaa ccgtttttca gcaggatttt actgatatcg
    12181 aaaaacgact tttacagagc ggcgaagatt atattgcgat gcttgaggaa gctcttgcgg
    12241 gttatggacg ttatctggat ctgctgcaag cgcgtgccga gccggattta atcattcagg
    12301 cgttcaatga gtaccaactt ttgtgcgccc tgcgggaagg gccgtttggc gtggctggac
    12361 tgaatgagcg aattgagcag tttatgcaac agaagcgcaa aattcatcgt catccgcact
    12421 ctcgttggta cgaaggtcga ccggtgatga ttgcccgtaa tgacagcgcg cttgggttgt
    12481 ttaatggcga tatcggtatt gcgctggatc gcgggcaggg gacgcgcgtc tggtttgcga
    12541 tgccggacgg caatattaag tctgtgcaac cgagtcgctt gccagagcac gaaactacgt
    12601 gggcgatgac ggtacataaa tcgcagggat cggagttcga ccatgcggcg ttgattttac
    12661 cgagccaacg cacgccggta gtaacgcgag agctggtata caccgcggtg acccgcgcgc
    12721 gtcgccgtct gtcgctgtat gccgatgagc gtatattaag tgcggcaatc gccactcgta
    12781 ctgagcggcg cagtggtctg gcggcgttgt ttagttcgcg ggaataaacg taattgccgg
    12841 atgcgacgca cgagtgttac gcatgtcgca tccgacaatt ttcatcgctt accctaaatc
    12901 cgccatcaac actttggatt tacgctggta gttgtacaac tgctttttgc tctcgggcag
    12961 taaatcaata tccaccgggg taaatccacg ttcctggaac cagtgaatac tgcgcgtggt
    13021 cagcacaaac aatttgctta agccgctctg cttcgcctga gcggcaatgc gttccagcag
    13081 aacctcgccc cgtgatgaac tgcggtaatc cgggtgaact gccacacagg ccatttcccc
    13141 aatcttctct tccgggaacg gatagagcgc ggcgcaggca atagtcgtgt tatcgcgctg
    13201 aataatggtg aatttgtcga tttccatctc cagctgctcg cgagaacggc gcaccagaat
    13261 accttgctgc tccagcgggc gaatcaactc cagaataccg ccaatatcgt tgattgttgc
    13321 gcgacgaatc tgctcggcgc tttccatcac aatctgcgta ccgataccgt cgcgtgagaa
    13381 caactcttgc aacagcgcgc catcttcctg ataactgatt aaatgacagc gacgcacgcc
    13441 gctgcggcag gctttcactg cgccacgcaa aaagcgcacc gtaccggagt tgtaatcgcc
    13501 tttctcttcc tgggcttcta cccgcgcttg cgcttcgtta gggaaaagtt ctgagacaat
    13561 atcaccgtcg tcattagtga cgccctgtga agagcaaaaa ccaatcatct tctcagcttt
    13621 cagtttgatg gccagttgag tggcaatctc ttccgaggtc agattaaagc tctcgccagt
    13681 gactgaaaca gcgaccggcc ccattagcac tattgcaccg ctgtccagtt gacgatggat
    13741 cgcgtcttca tcaatccgcc ggatacgccc gctatggcag taatccacgc cgtcatcgac
    13801 gcccagcggc tgggcaataa taaaattgcc actgacgacg ttgatatgcg cgccctgtag
    13861 cggcgtgtta ttgagactca tcgacaggcg agcagtgatg tccagttgca atgttcccgc
    13921 agcctgcttc accagttcca gtgttttggc gtcggtcaca cgtatattct tgtgatacag
    13981 cggttcgtgg tgatgcgcgg ccagatttgc gtcgatctgc ggacgtgcgc catagaccac
    14041 caccagacgg atgccgaggc tgtgcaacaa cccgatatca ttaacgatac tggagaaatt
    14101 ctcatgctca atggcttcac cgccgagcat gatgacaaac gtttttcccc ggtgggtatt
    14161 gatataggga accgaatggc ggaatccctc gaccagctcg gttttacgtt cctttaccac
    14221 ggcacacccc tttgcatgat tattctaaat tagtgtattt ttattctgtt ttttcgcagg
    14281 gtgcaagtgt aaattttatg cgagagcgac ttttttatca gtaatgccgt gaatataaaa
    14341 agaatgttta ccgtttatta gatgacagat tatgcgtctt tcgctaaagt ttccggtcaa
    14401 attagtcgtt tacttgttac acagcttaga tttcttttgc tcgggagagg catgtcagga
    14461 tccaacactg caatcagccg tcgtcgttta ctgcaaggcg cgggtgccat gtggctattg
    14521 agcgtaagtc aggtcagtct ggctgcggtc agccaggtcg tggcggtgcg cgtctggcct
    14581 gcgtccagct acacccgtgt gacggtagaa tcaaatcgtc agctgaaata taagcagttc
    14641 gcgttgagta accctgaacg cgtggtggtg gatatcgaag atgtaaacct gaactcggta
    14701 ctcaagggga tggcggcaca aattcgtgca gacgacccgt tcatcaagtc ggcgcgcgtc
    14761 gggcaatttg acccgcagac cgtacggatg gtttttgaat taaagcaaaa cgtaaaaccg
    14821 cagctgtttg cccttgcgcc ggtcgccggg tttaaagagc gtctggtgat ggacctctat
    14881 ccggccaatg cacaggatat gcaggacccg ctgctggcgc tgctggagga ttacaacaaa
    14941 ggcgacctcg aaaagcaggt gccgccagca caaagtggtc cacaaccggg taaagcaggg
    15001 cgcgatcgtc ctattgttat tatgcttgac cctggccacg gcggcgaaga ctccggtgcg
    15061 gtggggaaat acaaaacgcg cgaaaaagac gtggtattgc aaatagctcg ccgtctgcgc
    15121 tctctgatcg agaaagaggg caatatgaag gtgtacatga cgcgcaatga agacatcttc
    15181 attccgttgc aagtgcgcgt agcaaaagcc cagaaacagc gtgctgacct gtttgtctct
    15241 atccatgccg acgcctttac cagtcgtcag ccgagcggtt cctctgtgtt tgcgctctca
    15301 accaaaggtg caaccagtac tgcggcaaaa tatctggcac aaacccagaa cgcctcggac
    15361 ttgattggtg gcgtgagcaa aagcggtgac cgctatgtcg accacaccat gttcgatatg
    15421 gtgcagtcgc tgaccattgc cgacagcctg aagtttggta aagcggtact gaataagctc
    15481 ggtaaaatca acaagctgca taaaaatcaa gttgaacagg ccgggtttgc cgtactaaag
    15541 gcaccagata ttccctccat tctggtcgaa acggcgttta ttagtaacgt tgaggaagag
    15601 cgtaaactga aaacggcgac tttccagcag gaagttgcgg agtctattct tgcggggatt
    15661 aaagcgtatt ttgccgatgg ggcgacgctg gcgagaaggg gatgatgttt ttaatttatt
    15721 caatgaattt atgtttcatt gaataaatat tatttacata aatgttgaac gatggttttg
    15781 tgactgctgt cgaatatttg aatatcggct taaaaaataa gtctgtcatc tcttttatta
    15841 agcacaattc cgtgtaaggt atacgcatta gttaattatc ttgcaactgt taaatcaggc
    15901 aaggcaatgt ttgaagtaat tattacttct gacatgcctt gctttttttt ggggagccat
    15961 ggaatgatca tcgaaaaagt catgaacaat aattgtgtac aggcatcggt gaacggacag
    16021 gaggttatca tttctgggcc tggcgttggt tacaataaaa atatggtatg tcggtccctg
    16081 agcatcctgc aaaccggatt ttttatgtca gaaatgagca aaaaaataaa ctctacaaat
    16141 tgattgagca tgtgggtatt gagtatattt ttgttgccga aaagatagtg cattatgcgg
    16201 agagtaatct cgaaaaaaaa ctcaatccat cgctactact gattcttgct gatcacattt
    16261 cgaatgcaat atcccgagtc gtctcaggca tacaaattaa taatgttttc cttgaggaaa
    16321 tcaaagcgtt gtacaaagca gagtatgcaa taagtcgtga tgcattaaca atcattaatg
    16381 agcaatttag cgttcaacta ccggatgatg agattggttt tatagcattg catattttaa
    16441 ataattatga aaattcagtt gattatgaat cagtaaggat tattgagctg tctcaaaaaa
    16501 tcacggagct tattgaagtt gtttataaca gaagggtgga cagaagttca tttaactact
    16561 ccagatttat gatgcacctt aaatattttt ctagtcgtgt gttatgtaat gaaaaaataa
    16621 aacagaaaaa tattggtgat atctatgagc agtttcttga aaaagatttt cagttacaac
    16681 gtgccattca taaaattgaa agatatctgt acgcaacttt taaatatgat ttgattctgg
    16741 aagagaaact atatctttct attcgcatca aagtattaat ggactaatta tacataacat
    16801 tttcatgaga taaccagtat gaaacaaaag aaatcctgga gtttttttca gagccagggg
    16861 aaggcattta tgtatcccat cgctctgtta agtgtatgtg gtatgatgtt agggctggga
    16921 agtggtttag ccagcgatga tatggcaaaa ttcattccag tgttggctat tccgttaatt
    16981 aaaaccatac ttgattttat tgttagcctt ggtttgtttg ccttcgttaa tttacctgta
    17041 ttgtttgcga tagctatacc tttgggatta ttaaaagaga aagatgataa agcctatggc
    17101 gctttttctg gcttaattgg ctttatggcg atgcatcttg gaacgaactt ttatcttaaa
    17161 cagcacgact tattggtagc tgctgaccaa atgtcgacac atgggcaaac catcatactc
    17221 gggatccagt cctacaatac cagcgtgttg ggtgggattg tggcagggtt attagtcgcc
    17281 agcatgtata aaaagatcgt taacttacgt attcctgaat aattagggtt ttatagtggc
    17341 ccacgccttg tgcctatcat tacactgatt gtgatgagtg gatttggtct gatcattcct
    17401 tttatctggc cgccattttt caatcttttc atgctcattg gtcactggat ttcaacttcc
    17461 ggtcctgttg gttatttctt ctatgctgtt gccgaacgcg tgacgatacc ttttggctta
    17521 aatcacttgg tgacatcagt tttccgcttt accccaatcg gcggatcggc agtgattggc
    17581 ggtgaagagt attacggcac cctgaacatg tttatggcat acgtcaaaga gaatgcggtc
    17641 attccgctgg atttggcggg gaaaatggag cagggcaaac tgatgattca gtatggtctg
    17701 gctggagccg cgctggcgat gtatcgcact gctcatgctc aaaacagaag ggctatcaaa
    17761 gcattgctga tttccggtgt gctcacggta attattggcg gcgtcagcga gccgattgaa
    17821 tttctgttct tatttgtcag cccgctgttg tttgtcttcc atgcctttat gaatggattg
    17881 gctaacatgg ttctgccata tatgggcgtg aagatgggct ttactggcga tctgattcaa
    17941 tttattagct ttggtgtctt acgtggcaca agaacgggat ggccattagc ggtatgtgtt
    18001 gaagtggctt atttcttcat ttattacttt gtgttccgtt ggaccattct caaatttaac
    18061 ctgatgactg taggccgtga agagtccagt cctgtcacgc tgaacgctca cgaagaaact
    18121 gcaatagcta acatcccagt tcatgataaa tcagagttgc aggcagcgga gcaaatggtt
    18181 aaggctctgg gtggtaaaga aaatattaaa tcactggata attgtgtaac tcgtttgcgt
    18241 ttaactatcg cggatatggg attgataaat gaggctgcaa taaaaagggc cggaggaatt
    18301 gcggttgtga aacttgatca aaatacctta caagtcatta tcggtacaaa agtcatcgcc
    18361 ctacgtcggg atatggataa ttacatgggg atatgttgat ggatattttt aatacctcaa
    18421 tttctcgtaa aggaacatat tgcacacaat gggacttttg tgaagacaga tttggtgtaa
    18481 aagatgtatt accattctct atttcagata tggatcttcc gataccggaa gctatcatca
    18541 gaacattaaa aaaacgtctg gaacacccga ttttagggta cagtcgttgg cagcatgatg
    18601 actatctgaa ttcgatagtc aattggtatc atcaacaata taaaacagag attcaaccag
    18661 aatggatcac ctatagccca agcgtgatgt attcaatatc taaggcaata gaaatattga
    18721 cattgaatgg agataatatt ctcattttca cgcctgttta taacgcattt tttgatgtga
    18781 ttaagcataa tggtcgaaac atgctaacaa cctctttgat gaaagaaaat gggagaggct
    18841 acgttatcaa ctggcaagac tttgagctta aaattaaaag cgcgaagatg gtgttattat
    18901 gtaatcctca taacccgaca ggaacggtct ggtcggaaga cgatcttcac agaatcgcag
    18961 catgctgtgc aaggcataat atttggttat gttcagatga aatacatagt gattttgttt
    19021 ttaatcatag tttcacgtca gcgctaacaa taaaagatga caaagtggtg gtgtttaatt
    19081 cgatttcgaa aacatttaat gttcctgcgc taaccggatc atatatgatt tcaactgacg
    19141 ataattttaa tcataagttc agaacgatat cgaggtatcg cgatttcgta aactccccat
    19201 cagtactgaa tgtaattgcg accattgttg cctataatga atgtgaagaa tggttaaagt
    19261 cattaaaggt acatattgcc tctaacattc aatttacctt gcagtattta aatgaaaata
    19321 ttccggaatt gatcgtatgg ccagctgagg gctgctattt ttcgtgggtt gattgctcgg
    19381 cccttggtta tccctttgat aaattttacc gtcgattaat taacgaaggg agagtaggta
    19441 ttatggcggg gcatgtttat ggtactgaag gagagggcta tctgcgtctt aatctggcct
    19501 gcagtcgaga aaaattatat atggggcttg cgcgccttgc gagtgtaatt aaaaatatta
    19561 atcaggaaaa agaaaatgaa caatacggat cttatccatc ttattaaacg ttttatgcgt
    19621 aacgaacata aagcagtaga agaggttatt gattcccccc tttcggaagt cgcgaattta
    19681 attaaaatat tacaatcttg ccagggaaaa gttgttttta ttggtgttgg gaaatctggc
    19741 attattgcca gaaaacttgc tgcaacattt gccagcaccg gaactccctc gttttttgtt
    19801 cacggtacgg aagcggtaca cggcgacctt gggatggtgg cgaaagacga tgttgttatt
    19861 cttatttcaa acagtggtga gacggcggaa atcctggcga cactgcctag tttgaaaaaa
    19921 atgggtaatt atttgatctc ttttatccgc agtcatcact catcgcttgc gataagttgt
    19981 gatttatctg ttgaaatccc cgtcaaaagt gaggcggata attta
//
LOCUS       DADLTD010000060        19890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLTD010000060 DADLTD010000000
VERSION     DADLTD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.060"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..482
                     /locus_tag="JN701_001721"
     CDS             <1..482
                     /locus_tag="JN701_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364195.1"
                     /translation="ADTANALGSFINARRRLELRGEANGVTVYDDFAHHPTAILATLA
                     ALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHIPWQVAEVA
                     EACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLAKKAEAAQ"
     gene            complement(565..1116)
                     /locus_tag="JN701_001722"
     CDS             complement(565..1116)
                     /locus_tag="JN701_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY7364196.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            1210..2562
                     /gene="pmbA"
                     /locus_tag="JN701_001723"
     CDS             1210..2562
                     /gene="pmbA"
                     /locus_tag="JN701_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY7364197.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            2746..3132
                     /gene="cybC"
                     /locus_tag="JN701_001724"
     CDS             2746..3132
                     /gene="cybC"
                     /locus_tag="JN701_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY7364198.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALGAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(3177..3641)
                     /gene="nrdG"
                     /locus_tag="JN701_001725"
     CDS             complement(3177..3641)
                     /gene="nrdG"
                     /locus_tag="JN701_001725"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY7364199.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(3831..5969)
                     /gene="nrdD"
                     /locus_tag="JN701_001726"
     CDS             complement(3831..5969)
                     /gene="nrdD"
                     /locus_tag="JN701_001726"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY7364200.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(6363..8018)
                     /gene="treC"
                     /locus_tag="JN701_001727"
     CDS             complement(6363..8018)
                     /gene="treC"
                     /locus_tag="JN701_001727"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY7364201.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(8068..9489)
                     /gene="treB"
                     /locus_tag="JN701_001728"
     CDS             complement(8068..9489)
                     /gene="treB"
                     /locus_tag="JN701_001728"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY7364202.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(9590..10555)
                     /gene="treR"
                     /locus_tag="JN701_001729"
     CDS             complement(9590..10555)
                     /gene="treR"
                     /locus_tag="JN701_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY7364203.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            10740..10793
                     /gene="mgtL"
                     /locus_tag="JN701_001730"
     CDS             10740..10793
                     /gene="mgtL"
                     /locus_tag="JN701_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY7364204.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            10934..13630
                     /gene="mgtA"
                     /locus_tag="JN701_001731"
     CDS             10934..13630
                     /gene="mgtA"
                     /locus_tag="JN701_001731"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY7364205.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(13836..14222)
                     /gene="ridA"
                     /locus_tag="JN701_001732"
     CDS             complement(13836..14222)
                     /gene="ridA"
                     /locus_tag="JN701_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY7364206.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(14295..14756)
                     /gene="pyrI"
                     /locus_tag="JN701_001733"
     CDS             complement(14295..14756)
                     /gene="pyrI"
                     /locus_tag="JN701_001733"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY7364207.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(14769..15704)
                     /gene="pyrB"
                     /locus_tag="JN701_001734"
     CDS             complement(14769..15704)
                     /gene="pyrB"
                     /locus_tag="JN701_001734"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY7364208.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(15708..15842)
                     /gene="pyrL"
                     /locus_tag="JN701_001735"
     CDS             complement(15708..15842)
                     /gene="pyrL"
                     /locus_tag="JN701_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY7364209.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(16122..16517)
                     /locus_tag="JN701_001736"
     CDS             complement(16122..16517)
                     /locus_tag="JN701_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7364210.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(16648..17361)
                     /gene="bdcA"
                     /locus_tag="JN701_001737"
     CDS             complement(16648..17361)
                     /gene="bdcA"
                     /locus_tag="JN701_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7364211.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            17432..18025
                     /locus_tag="JN701_001738"
     CDS             17432..18025
                     /locus_tag="JN701_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7364212.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            18170..18622
                     /locus_tag="JN701_001739"
     CDS             18170..18622
                     /locus_tag="JN701_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7364213.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            18745..19890
                     /locus_tag="JN701_001740"
                     /pseudo
     CDS             18745..19890
                     /locus_tag="JN701_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         4850 a         4897 c         5176 g         4967 t
ORIGIN      
        1 cggcagatac cgctaacgcg ctgggttcgt ttattaatgc tcgtcgccgt ctggagttgc
       61 gtggtgaagc gaatggcgtc acggtatatg acgattttgc ccatcacccg acggcgattc
      121 tggcaacgct ggcggcgctg cgtggcaaag ttggtggtac agcgcgcatt attgctgtgc
      181 tggaaccgcg ctcgaatacc atgaaaatgg ggatctgcaa agacgatttg gcaccttcat
      241 taggtcgtgc cgatgaagtc ttcctgctgc aaccggcgca tattccgtgg caggtggcag
      301 aagtggcaga agcctgcgtt cagcctgcac actggagtgg cgatgtggat acgctggcag
      361 atatggtggt gaaaaccgct cagcctggcg accatattct ggtgatgagc aacggcggtt
      421 ttggtgggat ccatcagaaa ctgctggatg gtctggcgaa gaaggcggaa gctgcgcagt
      481 aattcggcct cagcctgaga tagcattgcc ggataaggcg tttacgccgc atccggcaaa
      541 ccatactcac atcaacaacg aaaattactc ttcattctcc gccagttcgc gcagatactg
      601 gaaaatctgg cgtgcggatt tcggcggctt attcccttct ttctctttct tcgcgttgcg
      661 gatcagagta cgcagttgct gacgatcggc atcgggccac agatttaata cctcagcgat
      721 ggcgtcatca ccttgatcga tcagacgatc gcgcaggttt tccagtttat ggaacagcac
      781 cacctgctgg ttgtgacggt ttttcagctt atccagcgcc tgacggatcg gttctacatc
      841 gcgctggcgc agcatcttac cgatgagctg caactggcgg cggcgacctt ccatcttaat
      901 acgctgggcg agctcaatag ccgcgcgcag atccgcatct aacgggatct tatccagcgc
      961 gtttttcccc agatcaacaa tttccgcgcc aaggcgtttt agctcctcgg catcacgttt
     1021 aatttcactt ttactgaccc agataatttc atcgtcttcg tcttcgatgt catcaccggg
     1081 aacgtcgtcg agccagtctt cgggctgctt agtcatctca ggctccttaa aaaaagaggc
     1141 taatgttacc agttaagatg cgcactgaaa aacggttctc tgttagactt cagagaaact
     1201 ctctacatta tggcacttgc aatgaaagta atctctcaag ttgaagcgca gcgcaagatt
     1261 ctggaagaag cagtttccac tgcgctggag ttggcctcag gcaaatcgga cggtgcggaa
     1321 gttgccgtca gcaagaccac cggcattagc gtaagcacgc gttatggtga agtggagaat
     1381 gttgaattca atagcgatgg cgcgctgggg atcactgttt atcaccagaa ccgcaaaggt
     1441 agcgcatcat ccaccgattt aagcccgcag gccattgccc gtactgtaca ggcggcgctg
     1501 gatattgccc gttatacctc gccagatccc tgtgccggcg tggcagacaa agagctgctg
     1561 gcctttgacg caccagatct cgacttgttc caccctgcgg aagtttcccc ggatgaagcc
     1621 attgaactgg cggcccgcgc agaacaggcg gcattgcagg cggacaaacg catcaccaat
     1681 accgaaggtg gcagctttaa cagccactac ggtgtcaaag tttttggcaa cagccacggc
     1741 atgttgcagg gttactgctc aacgcgtcat tcgctctcca gctgtgtaat tgccgaagaa
     1801 aatggcgata tggagcgtga ttacgcctac accattggtc gtgcgatgag cgatctgcaa
     1861 acgccagagt gggttggggt cgactgtgct cgccgtactt tatcgcgcct gtcaccgcgt
     1921 aaactctcca ccatgaaagc gccggtcatt tttgccaatg aagtggcaac cgggcttttt
     1981 ggccatctgg tgggggcgat agcgggtgga tcggtttacc gtaaatctac cttcctgctg
     2041 gattcgctgg gtaaacaaat tctgccggac tggctgacca ttgaagagca tccgcatctg
     2101 ctgaaagggc tggcgtcgac gccattcgac agcgaaggtg tgcgcaccga gcgtcgcgat
     2161 attattaaag atggcatcct gactcagtgg ctgctgacca gctattcggc gcgaaaactg
     2221 gggctgaaaa gcaccggaca tgcgggcggt attcacaact ggcggattgc cggacaaggt
     2281 ctaagcttcg agcagatgct caaagagatg ggcaccgggc tggtggtgac ggaattgatg
     2341 ggccagggcg tgagtgcaat taccggtgat tattcccgtg gtgcggcggg cttctgggta
     2401 gaaaacggcg aaattcagta tccggttagc gaaatcacca tcgcaggtaa tttaaaagat
     2461 atgtggcgca atattgtcac cgtcggtaac gatattgaaa cacgcagtaa tatacagtgt
     2521 ggttctgtgc tgttgccgga gatgaaaatc gccggacagt aaaaaaactg gcgcgacctg
     2581 tcgcgctata aactaaaaaa ttacacaaaa tcattcgcac tgcatcgaga cggaaagtga
     2641 atgagggccg gggagcgtac tcgcagtacg tgacccggtc gaatgagcgt agtcgtcgat
     2701 gaggcagtgt gaatgatgaa gtgtaaataa aaaaggaagt gagcaatgcg aaaaagcctg
     2761 ttagctattc ttgcagtctc ctcgttggta ttcagttcgg cgtcgtttgc cgctgatctt
     2821 gaagacaata tggaaaccct caacgacaat ttaaaagtgg tcgaaaaagc cgataacgcg
     2881 gcgcaagtca aagacgcgtt aacgaagatg cgcgccgcag cgctgggtgc gcaaaaagca
     2941 acgccgccga agctcgaagg caaatcaccg gacagcccgg agatgaaaga cttccgtcac
     3001 ggtttcgata ttctggtcgg tcagattgac gacgcgctga agctggcaaa tgaaggtaaa
     3061 gtaaaagaag cgcaggctgc cgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag
     3121 aagtatcgtt aattcctcat ttccctgttg cctgcactca ggtaacaggg aacttatcat
     3181 cgcaaatgat gcaccacctg attgctgctg ccgcgccaga tcagggaagg gtcttttaaa
     3241 tcctgcacaa atttgccgtc gaccagcacg ttaatcaaat caacaacctg catttgcgct
     3301 gcgttgagtt cgtcgagttt atagcctgtc cacacccaga tgtctttacc cggacactcg
     3361 gcgcggatgc gttgtaccag tttcagaata tccggcacgt tttgcggatg cagcggatcg
     3421 ccgccggaga gggaaatacc ctggcgtttg atacgagtgt cattcagatc gttaatgatc
     3481 tggtcttcca ttgctttagt aaatggctga ccggaattta cccgccatgt gcttttgtta
     3541 tagcaaccgg ggcattcatg aacacacccg gagacaaaca gagtgcaacg agtgccgggg
     3601 ccgttgacga tgtcgacagg atagtactga tgataattca ttatttcgcc tggcttatca
     3661 gtccgcgtgt agactgcatc cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca
     3721 ggcctacagc cgttgccaaa tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca
     3781 acgcgggatg cgacgctttc acatcacatc cggcatttgc ttagtgcaga tcaacctatc
     3841 tgcccatttc ccaaatgttt aacgcggcgc ttaacttctt cctgcttgcc ggcgttaaac
     3901 ggacgtgcat ccgggctacc taaatatccg cacacgcggc gagttaccga cacacgggag
     3961 gcgtcatggt taccacattt cgggcaggtg aagcctttgc tggtgcactc gaactcaccg
     4021 gtaaagccac actcgtagca ctcatcaatc ggtgtattgg tgccgtaata cggtacatgc
     4081 tgatagctgt aatcccagac atcttccagc gccttcaggt tgtgctgaat gtttgaatac
     4141 tcgccgtagc aaatgaaacc accgttcgcc agcggcgggt aaggcgcttc aaagtcgatc
     4201 ttgtcgtacg ggttcacctt cttctccaca tcgaggtgga aactgttggt gtagtaacct
     4261 ttatcggtca cgcccggcac cacgccaaac tcagcagtat cgagacggca gaagcggtcg
     4321 cacaggtttt cactcggcgt gctgtagaga ctgaaaccat aacccgtttc ttccttccac
     4381 tgatccactg cctgacgcag acgttcaaca atggcgatac ctttcgcgcg aagctgctcg
     4441 ttgtcgtaaa catgctcgcc gccgaacagc gcgttaatgg tttcgtggat gccgatgtaa
     4501 cccagcgaaa tagacgcacg accgtttttg aagatttcag aaacatcatc gtcagcattg
     4561 agacgcacgc cacaagcacc ttccatatag aggatcgggg ccacgcgcgc tttcacgcct
     4621 tcgagacgag cgatacgggt catcagcgcc ttacgtgcca gcaccagacg ttcatccagc
     4681 agcttccaga aggtggcttc atcgcctttt gcttccagag caatacgcgg caggttcagg
     4741 ctgatcacgc cgaggttgtt acgaccatcg tggatctgct cgccgttttc attttcccac
     4801 acgccgagga agctgcggca gcccatcgga gttttaaacg aaccggtgac tttcactacc
     4861 tgatcgtagt tcaggatatc cggatacatg cgcttgcttg cgcactccag cgccagctgt
     4921 ttgatgtcgt agttcggatc gcctttttta tggttcaggc catcgcgaat cgcaaacacc
     4981 agtttcggga acaccgcagt tttacggttt ttaccgaggc ctgcgatacg gttacgcagg
     5041 atagattcct gaatcaggcg cgactcccag ctggtgccca gaccaaaacc aaaggttaca
     5101 aacggcgtct gaccgttggc ggtgtgcagg gtgtttactt cgtactccag cgactggaag
     5161 gcatcgtagc actctttaat ggtacgagag ttagcgtagc cttcggcgtc cgggatgttc
     5221 cactcttctg cggtcttgcg atgtttgttg tagctggcgg tgacaaacgg tgccagcact
     5281 tcatcgatac ggttaatggt ggtgccgcca taaatatggc tggcgacctg agcaataatc
     5341 tgcgcagtta cagcggttgc cgtagagatc gacttcggcg gttcaatctc ggcgttcccc
     5401 atcttaaagc cctgggtcag catgcctttc aggtcgatca acatgcagtt gaacatcggg
     5461 aagaacggcg agtaatcgag atcgtgatag tgaatatcgc cacgctcatg tgcctgcacc
     5521 acgtcacgcg gcagcaggtg ctgacgtgca tagtgtttag ccacgatccc ggccagcagg
     5581 tcgcgctggg ttggaatcac cttgctgtct ttgttggcgt tttcgttgag taacgaggcg
     5641 ttggtctgct cgaccagacc acggatctcc tgattcaggc gaccgcgttt ttcacgttca
     5701 atgtcgcgat cgtgacggta ctcgatgtaa gcacgagcca gttgtttgta tggacctgac
     5761 atcagctggt tttcaacagc ggtctggatc tcattgatat ccacctggtt gcggccctgc
     5821 atctgctcgc tgacaactgc agcaacagtg gcgcaataat cggcatcatc gacttccgct
     5881 gctttagctg cacgcagaat cgcttctttg atgcgctctg atttaaacgg cactttgcag
     5941 ccgtctcgtt tcatcacatg cggtgtcatg atcgctccat atttttaaga acaggttatc
     6001 cacagaaatt gggaaaggcg ttttccggtt ttttcgtttc gttttccggt gctttccgca
     6061 atcgccatcc gcttttccac aacgccgcac ctttttcgtg gggagctgta gtagcaatta
     6121 tagtcgatta atacaacata ttgggttggg acgcatttta aagtctatat atagtgcttt
     6181 gcatcaagga tgtttgaact ttttttgatg tagctcaaag taaaaagcag agcgtacgga
     6241 tgacgggcgc tacagcgtta tgtaaatttt ttaatgaatt tgctggttga aaaataaaca
     6301 aaaacaacat gctgacagac aaaaccccgg aatgaccggc attaccgggg cttagggaag
     6361 atttacttct gtaaccacca gacagcctca aaaggccgta aattcatggc acagggttgt
     6421 ggtgaggctt cttcgtagtt atgcatcaca agctgccagt tgccgcgcat ttgccctggc
     6481 tgccagggtt ggatctcacg gctaaggttg gcaatgacca gcaaggtttg ccccttccat
     6541 tcacggcgat agcaccacaa tacagggctg tttggcagca gatcctggta attgccccat
     6601 gtcaggatgg cttcctgctt acgcagtgcg attaactttt ggtaggtgta aaacaccgag
     6661 gaatcatcgg ccagcgcggc ttctacgttg atttgttgat agttatcacc caggccaatc
     6721 cacggttcgc cagccgtaaa tccggcatta tcgccgttgc tccattgcat gggcgtgcgg
     6781 ctgttgtcac gggatttact ggcgaggatt gccaataact cgtcggcatc acgaccatcg
     6841 ttgcgcagct cggcaaacat attgaggctc tctacgtcgc gatagtcagt aatgcgcgtg
     6901 aaatgcgggt tggtcatgcc aatctcttcg ccctggtaga tatacggcgt tccctgcatg
     6961 ccatgcagca ccatcgccag catttttgcc gcaggcacgc ggtattcacc ttcatcacca
     7021 aagcgagaaa caatgcgcgg ctgatcgtgg ttacaccaga acaaggcatt ccatgctacg
     7081 ttgtgcattc cttgttgcca gtggcggaac aatgttttca acgccacaaa gtcaggttta
     7141 gccagcgtcc atttttcacc accgggataa tcgaccttca ggtgatgaaa attaaaggtc
     7201 atcgacaatt cactgcctgt cagtgccgcg tatcgctggc aatgctcaag gctggtggag
     7261 gacatttcac ctacggtcat taacccgcgt ggcgtaaaca catcgcggtt catctcgtgc
     7321 aaaaactcgt gtgctcgtgg cccgtcggtg tagaagcgac gcccgtcgcc atccaggtca
     7381 tcagggaaac gcgggtcttt ggagatcaga ttcaccacat ccaggcgcaa cccgtcgacc
     7441 ccacgatcgg cccagaactc acagactttt ttcagcactg cgcgtaccgc tggattctcc
     7501 cagttgagat ccgcctgttc tggtgcaaag agatgcaaat agtactgttc gctttccgca
     7561 tgccagcgcc acgcactacc gccaaatttt gaacgccagt tgttcggtgg cgtttctggt
     7621 tctccatcgc gccagatata aaactggcgg taagggcttt ctttgttcag cgcctcgcga
     7681 aaccaggcat gttgggtaga ggtatggtta aacaccatat cgagaatgat acgaatcccg
     7741 cgcgattttg cctgcgtcac cagttcgtca aaatcgtcca gcgtgccgta ggtgggatca
     7801 atcgccgtat agttcgctac gtcgtaaccg ttatcgacct ggggagagac ataaaagggg
     7861 gttagccaga tggcatcaac gcccagttta tgcagatagt ccaggcgttg gataacgcca
     7921 cgtaaatcgc cggtaccgct acccgtggtg tcctgaaaac tctttggata aatctggtag
     7981 ataacgccgt tttgccacca gtggggagga ttagtcatta cgttattcct gcaaatgcga
     8041 gggggcgcaa ttgcgccccg aagaaaatta aacaatgtcc agcgtgccca ggcggtattt
     8101 ccgctgatag ataaacgagg tgagtacaat cgggatgatg atggcgatag ccattgccag
     8161 cgcaaacacc tgccagtagt tcggttgaat cgagagaatt cccggcaggc cgcctacgcc
     8221 gatgccattc gccataacgc cgttcaggcc gcatagcaat cctgccagac cagaaccaat
     8281 catcgcgcac agcatcggga agcgatattt caggttgatg ccgtacattg caggctcagt
     8341 gaccccaagc caggcggaga tcgccgcagg cacggagatc tcgcgttcat tgtgcttgcg
     8401 gctggaaatg ataatgccga tcacggcgga gccctgagcg atattcgaca gcgcaatcag
     8461 tggccacact ggcgtaccac ccatgctttg aatcatctgc aaatcaatag caagcgtggt
     8521 ctggtgtacc ccggtgatca ccagcggggc gtacaggaag ccaaacaatg ccgcgccaat
     8581 cggagcaaag ctgccggtca tcaggtgacg taccgcaaag gcaacgccat cgccaatcat
     8641 gcgaccaaac ggaccaatca gcgcatgggc gaggaacacc gcgaggatca gcgaacagac
     8701 gggcaccacc accagataga ggtaatctgg cacgatgcgt ttaaggcgag tttcaataac
     8761 gcccagcgcc agcccggcta acagtgccgg gatcacctgc gcctgatagc ccactttggc
     8821 gatgctgaac atgccaaagt cccacacttc cggcagctgc tgcccgagca gataagcgtt
     8881 catcagttgg ggggaaacca gtgtcacgcc aagcacgata ccaaggatcg gtgtgccgcc
     8941 catttttttc accgctgacc agcaaatccc gaccggcagg tagaagaaga tcgcttcacc
     9001 gatcaaccac agaaaatcgt agatcgtttg cagggaaggg tacatttgcg ccagcgtttg
     9061 accgttgctc atgggcaaat cgccgatcac attgcgaaaa ccgaggatca aaccgccgct
     9121 aatcaacgcg ggcagcaacg ggaagaaggt ctctgcgaaa tgagagatca actgctcatg
     9181 ccatttcata ttctgccggg cggctttttt tacctgctct ttatcaacct gcgcctgtcc
     9241 ggttgacgct atcagtgctt gatagtagtc acccacgttg gtgccaatca ccacctgaaa
     9301 ttgcctggca ttggtgaaac agcctttcac catggggagt tgctcaattt ctttcggtct
     9361 ggcattggcc ggttggttga ggacaaagcg taggcgagta atacagtggc tcaccgtcgc
     9421 aatattgccg cgcccgccga ccagttcaat caaccgatcg atatccgttt ggtttatttt
     9481 gctcatcata aagccccatg gcagatgaca tttttggttg gctgcagaat atattgcgcg
     9541 gaaaaattta aaaacgggaa cgttcccgaa acgcagcgaa gatcacaatt tatcgttcag
     9601 gaaacgatct ggacagggtg gcggggatga tgatttgttg cggttcgctg cgcccggtta
     9661 cctgtgcgat caactggcag gccgcctggc gtccagcttc ggcgtaaccg ggatctacgg
     9721 tgacgatctc cggatggagg aatttcatta acggcgtatt accgacgctc gccagttgca
     9781 aggtgtcgat gcgttgctct tgcaggtatt tacttgcgcc aagtgccagc gtgtcggttg
     9841 cgcacagtaa ggcggtagtt tcaggcgtaa tcacttttgc tacgttctca tagccttgct
     9901 tcatagcaag gcctggcaga gcggcaacgg gatgcagttt atgcgctttg cagaacgcca
     9961 ggtaggcttc gtgacgtcgc ttaccggtcg tcacgtcact gtgcggcacg ccgagataac
    10021 tgatattacg atgcccctgg tcatacagcc gttgcatcag gatcttgatt gccccttcgt
    10081 cgtcataaca gaccgaagca aagccttttg cgtcacgcgc cagcagaacc agcgatgact
    10141 gccagtgggc taacatttct tctgttatgc cggtaaaacc gaacagcact acgccgtcga
    10201 tattacgccg tttcagcacc cccaaatgtt cggcaactaa ttgcggggaa aactgacttt
    10261 ccatcatgat tgggtcgtaa ccttgttcat agaactctgg cagcatggtt tgaacggcga
    10321 gattttctga caacgaatcc aggcgggtaa caatgatggc gaccacttta tcgctttgcc
    10381 cacgcatagc gcgcgcagag cgggaagggg aaaatccatg ctgattcatc actgcttcaa
    10441 cacgctcgcg ggtgcgctgg ctcacgccgc tttcgttatt cagcacccgg gaaactgtag
    10501 atttccccac gccgcttaag cgcgcgatgt ctttgatggt cagccgattt tgcatcctgt
    10561 tgtcctgtaa cgtgttgttt aattatttga gcctaacttt acccgtgcat tcagcaatgg
    10621 gtaaagtctg gtttatcgtt ggtttagttg ccagcaggta ttatatcgcc atagatgcta
    10681 cgaatattat tggattctcc ttattatttg cggcgctttt ttcacttacc ggaggttata
    10741 tggaacctga tcccacgcct ctccctcgac ggagattaaa acttttccgg taagcccgtc
    10801 ttttcacggc gttaccggat gcgtaaggcc gtgacgtttt aacgtccctg ctcagcttta
    10861 ttaccttcag gtaaggcttt gccacgcctg aagacatttc tgtactgttt cagacagtgc
    10921 ggagggactc cttatgttta aagaaatttt tacccggctc attcgccatt taccttcccg
    10981 tctggttcat cgtgatccat tgcctggcgc gcagcagaca gtgaatacgg tggtcccgcc
    11041 gtccttaagt gcgcattgcc tgaaaatggc ggtgatgccc gaagaagaat tgtggaaaac
    11101 gttcgacacc catccggaag ggttaaatca ggcggaagtg gaatctgccc gcgaacaaca
    11161 tggtgaaaat aaattacccg cacaacaact gtcgccgtgg tgggtacatt tatgggtctg
    11221 ctatcgcaac ccctttaata ttttactcac cattctcggc gctatttctt acgccacgga
    11281 agatttattt gctgcgggcg ttatcgcgct aatggtcgct atttctacgt tgctgaactt
    11341 tattcaggaa gcacgttcca ctaaagcggc agatgccctg aaagcgatgg tcagcaatac
    11401 cgcgacggtg ctgcgcgtaa ttaacgacaa aggcgaaaat ggctggctgg agatcccgat
    11461 cgaccagctg gtgcccggcg atattataaa actggcggcg ggggatatga tcccggcaga
    11521 tttacgtatc ttgcaagcgc gggatctgtt cgtcgctcag gcgtcgttaa ccggtgagtc
    11581 tctgcccgtg gaaaaagccg ctaccactcg ccagccggag cacagcaatc cgctggagtg
    11641 cgacacgctg tgttttatgg gcaccaccgt ggtgagcggc acggcacaag caatggtgat
    11701 tgctacaggt gccaatacct ggtttggtca actggcgggg cgtgttagtg agcaggaaag
    11761 cgagccgaat gcctttcagc aagggatcag ccgcgtcagt atgctgctga ttcgctttat
    11821 gctggtgatg gcaccggtgg tgctgttaat caatggctac accaaaggcg actggtggga
    11881 agcggcgctg tttgcgcttt cggtcgcggt aggcctaacg ccggaaatgt tgccgatgat
    11941 tgttacctcg acgctggcgc gcggcgcagt aaagctgtcg aaacagaaag tgatcgtcaa
    12001 acatctggat gctattcaga actttggcgc aatggatatt ctgtgcactg ataaaaccgg
    12061 caccctgacg caggataaaa ttgtgctgga gaatcatacc gatatctccg gtaaaaccag
    12121 cgaacgcgtg ctgcatagcg cgtggttgaa cagtcattat cagaccggac ttaaaaacct
    12181 gctcgataca gcggtgctcg aaggtacgga tgaagagtca gcgcgctcgc tggccagtcg
    12241 ttggcaaaaa atcgatgaga ttccgtttga tttcgagcgt cgccggatgt cggtggtagt
    12301 ggcagaaaat accgagcacc atcagctggt ttgcaaaggt gcattgcagg aaatcctcaa
    12361 tgtgtgttcg caggtgcgtc acaatggcga gattgtgccg ctcgatgaca tcatgctgcg
    12421 taagattaag cgggttactg atacgctgaa tcgtcagggg ctgcgcgtgg ttgcggtggc
    12481 gacgaaatac ctgccagcgc gtgaaggaga ttaccagcgg gcggatgaat ccgacctgat
    12541 cctcgaagga tatattgctt ttcttgatcc gccaaaagag acaaccgctc cggcactgaa
    12601 ggcattaaaa gcgagtggaa ttaccgtaaa aatccttact ggcgacagtg agttagttgc
    12661 agcgaaagtg tgccatgaag tggggctgga cgcgggagag gtggtcattg gtagtgatat
    12721 tgaaacgcta tctgacgacg aactggcaaa tcttgcacag cgcaccacgc tgtttgcccg
    12781 cctgacgccg atgcataaag aacgcattgt gaccttactg aagcgcgaag ggcatgtggt
    12841 tggctttatg ggcgatggta ttaatgatgc gcccgcctta cgcgcggcgg atatcggcat
    12901 ttctgtggac ggcgcggtag atattgcccg tgaagcggct gatatcatcc tgctggaaaa
    12961 aagcctgatg gtgctggaag agggggttat tgagggacgt cgcactttcg ccaacatgct
    13021 gaaatacatc aaaatgacgg cgagttctaa ctttggtaat gtgttcagcg tgctggtggc
    13081 gagtgctttc ttgcccttcc tgccgatgtt gccgttacac ttgcttattc agaacctgct
    13141 gtacgatgtg tcacaggtgg cgatcccgtt tgataacgtc gacgacgagc aaattcaaaa
    13201 gccgcagcgt tggaatccgg cggatctggg gcgctttatg atcttcttcg gaccgatcag
    13261 ctcgatcttc gatattctga cgttttgcct gatgtggtgg gtattccatg ccaacacgcc
    13321 ggaaacgcaa acgctgttcc agtcgggatg gtttgtggtg ggcttactgt cgcaaacgct
    13381 gattgtgcat atgatccgca cccgccgtgt gccgtttatt cagagctgtg catcgtggcc
    13441 gttaatgatc atgaccgtga tcgtgatgat tgtcgggatc gcattgccgt tttcaccgct
    13501 ggccagttat ctgcaattac aggcgttgcc gttaagctat ttcccgtggc tggttgcgat
    13561 tctggcaggg tatatgacgt taacccagtt ggtgaaagga ttttatagcc gtcgttacgg
    13621 ctggcagtaa agaataaact gggcatggtg gcccggattc agattgcaga caaagtgagt
    13681 tttgttcatg ccggatgcgg cgtaaacgcc taatccggcc tacaaattca atcaattgca
    13741 ggaatcacgt aggcctgata agcgtagcgc atcaggcgat tttgcgtttg tcatcagact
    13801 taatccgggc atgatagccc ggatttccat caagattagc gacgaatagc gatcgcttcg
    13861 atctcaatct ttacgtcttt cggcagacgg gcaacttcaa cgcaagaacg tgccgggaag
    13921 gtggcgttgt gttcggtgaa gaaggcttcg taagtggcgt ttacggttgc gaagtcgttc
    13981 agatctttta caaacacggt agttttaacg atgtcgccca ctttcagacc agcggcttcg
    14041 acgatcgctt ttacgttatc cagcgactga cgtgcctgtg cagcgacgtc tgccggtact
    14101 tcgcccgttt tcggatttac cgggatctga ccggaggtga tgatcatatt tcccagatca
    14161 acgccctgta cgtaaggacc gatagctgct ggtgcatttt ccgtcgcgat agttttgctc
    14221 atgatttctc ctttattaca gcggtaaaaa gtctggctca ttatagggag ccagactttt
    14281 attaccaacc gcaattaatt ggccagcacc acattatggg aaaactcttt ttcacagtat
    14341 ttgcatttga gcgcgatgtc attggcgcgt tttcgcacgg caaagctgga tgaaaccggt
    14401 tcggcatggc tgatacagtt gctgttcggg cagaccaaca cattgtcgat gcgctctggc
    14461 agactggggc gcgatttacc caccacttca tagttgtcga tacggttaac cgtggcttgc
    14521 ggcgcataca atgccagttg atctacctga tcttcactca aaaaggtatt ttcgattttg
    14581 atcagatctt tgcggcccat ctcgccagaa ggcaggttca gaccaatggt gatgcgctga
    14641 tccgtttcgg tcagcttgaa cagactcaac agcttaaaac cgatctgggc ggggatatgg
    14701 tcaattaccg tgccgcgttt aatagcttca acctgcaatt tattatcgtg tgtcatctct
    14761 atttcccctt acagtaccag atcgcgattc agaaccagtg ccagtaacgc ctggcgagcg
    14821 aaaatcccgt tgcctgcctg ctggaagtac caggcgtgtg gcgttttatc aacatccgtc
    14881 gcaatttcat caacacgcgg cagcggatgc agtactttca tattggcttt ggcgttgtgg
    14941 agatcgctgg cgcgaagaac aaactgcgct ttcacgttgg cgtattcgga tgggtccagg
    15001 cgctcttttt gcacgcgggt catatacagg atgtctactt ccgccatcac ttcttcaata
    15061 gagctgtgca gactccatgc gatccctttt tcatcgagca tatccagaat gtattgcggc
    15121 attgccagcg cgtccggcgc gatgaagtaa aaacggttgc cgtcgaactt cgctaacgcc
    15181 tgagtcaggg agtgaacggt gcggccatat ttcaggtcac caaccattgc gacgtggaga
    15241 ttgtccagac gcccctgggt ttcctgaata gtgaataagt ccagcaaggt ttgcgtcgga
    15301 tgttggttgg agccatcacc ggcattcagt accggtacat tgccggaaaa ctcggtggcc
    15361 aggcgcgccg caccttcctg cggatgacgc atcactatcg catcgacgta agtgctgata
    15421 accgaaatgg tatcggccag cgtttcgccc tttttaccca gtgatgtatt ggcgctgtcg
    15481 gagaagccca ccacgctggc acccaggcgg tgcatggaag tttcgaaaga gaggcgggta
    15541 cgggtagagg cttcgaagaa gcagctggca atgactttgt gcttcaacag ctctggttgc
    15601 gggtttgctt tcagttttgc cgctgttgcc agcaccagat taaggtcatc gcgactaagg
    15661 tcgtttatgg aaatgatatg tttctgatat agcggattag ccatctttta tctcctgacg
    15721 cctgggcaaa aaaaagcccc tcgattgagg ggctgggaat gggtgatcaa cgggaagaaa
    15781 aacggcaggc cagcgtcttt tttcagacgc ggtaagacaa aatgtcgaac acactgaacc
    15841 atacatcctc ccggcaaatt gtccggcatt atactcatcg tcagaagcgg tgcaagcaat
    15901 tgatgcaata ttttgtcagc gcaaacggtt tatttgaatt taaagttaaa atatatgcat
    15961 ttttatttac tgtgattctg gggggaactg tcgttcggta tcaataattg cagacatttc
    16021 ctgctttttt ctttttcact ttcacgcaat cagatatgca ttttattcca ttcatccgac
    16081 ttatagggcg gagtgtgaaa acgaacggct agcactattg cttagagctc agggatgcgc
    16141 gctatcactt taatttcaaa atcaaagcct gccagccatg taacgcccac cgccgtccag
    16201 gttggataag atggcgcgct aaatatttca tttttcaccg tcatgatgtc ttcaaattgt
    16261 ttttctggat cggtatgaaa gctcgtaaca tcaatgatat cgtcaaaagt gcatcccgca
    16321 gctgccaggg tcgcatgcaa attatcaaag gccagtctga cttgttgctg aaaatcgggt
    16381 tctggtgttc cgtcctctcg acttcctact tgcccggaaa caaacagcaa atcgccggaa
    16441 cgaatagccg cagaataacg atgctcagca tacagtgaat gtcgtccagc agggaaaaca
    16501 gcggttcttt ctaccatttg gttatcctca agatttacga catgaacaga agatttatat
    16561 ttaccaggag ccgcttttag cggacgacgt gagtaaacaa aacccagaca tcatgggaaa
    16621 tggctgggct taattgagcg tagtcggtta tgcgccaaac gcgccatcaa tggtatgcat
    16681 cgcgccggta acaaaactgg cttctggccc tgctaaccat gcgaccatac cagcgacctc
    16741 ttccggttgc ccatgtcttt tgatggccat aaaactatgc gacatatcgc gcattggccc
    16801 gttggcggga ttagcgtcgg tatcaattgg ccctggctgg acgacgttga tggtgatccc
    16861 acgcggtccg aaatcacggg ccagcccgcg cgccatgcct tgcagggcag atttactggc
    16921 ggcataagca gccatgcctg caacaggcat acgatcgcca ttcacggagc cgatgattaa
    16981 gatgcgtccg ccttcgggca tctgccgggc ggcttcaaca gaggcatggt aaggagcatg
    17041 aatattgatt ttgaaaaggc gatcaatatc gtcggcattt aattccaggg catcgccaaa
    17101 gacgccaata cctgcattta ccaccaggat atccaatgcg ccgctcttac gaacgacatc
    17161 aataacagcg tctttgtcag cactatctgt gaatactgct gtcgctccag tctcttgtgc
    17221 caggcgttca gcggcatctt tcgaccccgc ataggtgaat cgtacattgg ccccatcggt
    17281 gacgaaacga cgtacgatag cggcaccgat tccacggcta ccaccgagga tgagaactgt
    17341 cttacctgta aaagcgccca taaggactcc ttgatttatt atgtaacatg cattacaaaa
    17401 ctgttttaac tttctgtcaa caagttttgt aatggtcact aaaaaacaat ctcgcgttcc
    17461 aggtcgtccc agacgtttcg ctcctgagca ggcagtctct gcggcaaaag tgctttttca
    17521 ccaaaaaggt ttcgatgctg tcagtgttgc tgaagttact gattatcttg gtattaaccc
    17581 cccgagcctc tacgcggctt ttggcaataa ggctgggtta tttagccgtg tactcaatga
    17641 atatgtcggt acggaagcta ttccgcttgc cgatattctt cgtgatgatc gtccggtagg
    17701 ggagtgcctg gctgaggtat taaaagaagc ggcgcgccga tatagccaaa acggcggctg
    17761 cgctggctgt atggttcttg aaggcattca tagtcatgat ccacaagcgc gtgatattgc
    17821 cgttcaatat tatcacgccg cagaaacgac catttatgac tatatcgcca ggcggcatcc
    17881 acaaagcgca caatgtgtga ctgattttat gagtaccgtg atgtcagggc tttctgcgaa
    17941 ggcacgggag gggcactcca tcgaacaact ctgtgcaaca gctgcactgg cgggggaagc
    18001 gataaaaact attctcaagg agtgatgcgg gccttgaccc gaaaggcggg aacgcgcctg
    18061 ccgataagtt gtgataagac aataattcac gcgttaaggc tagcggaatt gatcatcttt
    18121 tcgtataacg atggaaatga aacgttgttt taattaagga gtggaaaaga tgatcatcgg
    18181 aaatattcat aatcttcagc cgtggctacc ccaggagtta cgccaggcga ttgagcatat
    18241 caaagcacac gttacggcag aaacgccaaa gggcaagcac gatatcgaag gcaatcgtct
    18301 gttttatctt atctcggaag atatgaccga gccgcacgaa gctcgccgtg cggagtacca
    18361 tgcccgctat ctcgacattc agattgtgtt aaaaggtcag gaaggcatga ccttcagcac
    18421 gcaacctgca ggcaccccgg ataccgactg gttagctgat aaagacatcg catttttgcc
    18481 ggaaggcgtt gatgagaaaa cagttatcct taatgaaggt gattttgttg tgttttatcc
    18541 gggggaagtg cataaaccgc tgtgcgcagt gggcgcgcca gcccgggttc gcaaagcagt
    18601 agtgaagatg ctgatggcgt gatgactttt cgccgtaaat aactccaggt ttacggcgag
    18661 tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa aatttgtaat gcttagacgt
    18721 tcttattaac tcaaggagtt cgtcatgagc aaaatatcag atttgaatta ttctcaacac
    18781 attacattag ccgacaattt taaacaaaaa agtgaagttt taaatacctg gcgtgttgga
    18841 atgaataatt ttgcccgtaa tgccgagggg caggataaca caagaaatat ccttgatcct
    18901 aagacatttt tggaattttt ggtaaaaata tttactctag gttatgtgga ttttagcaaa
    18961 cgctccaacg aagcgggaag aaatatgatg gctcatattg agtcctcatc ttatatcaaa
    19021 aataatgatg gcagtgagat aataaagttt gttatgaata atcctgaagg ggaacgagcg
    19081 gatttatcta aggtggaaat agaaatcaca ctttccactt tcactactat ggggactcgt
    19141 caaggacata cggctattat atttcaacaa cctgatggtt cgactaatcg ttatgaagga
    19201 aagtcctttg aaagaaaaga tgagagttca ttacacctga ttactaacaa gattctggca
    19261 tgttaccaac gcgaagctaa caaggaaata gctcgtctat taaatatttc acaggagtta
    19321 aataattctc aggacttgaa taactcgcag gtgagttgta aagattcagt tgattctacg
    19381 attacggatt tattagaaaa accattgaat aatgcattat tagcaataag gaaagaacat
    19441 ctgctattaa tgccttatgt atgtaatgaa tcgatttcat acttgcttgg tgaaaaaggg
    19501 atactgaaag aaatagatga ccttaacgca gtaaataatt atcttcttaa taagaagaaa
    19561 gctactgaca atgaaattaa tgacatcaaa gttaacctgt ctcatattct tattgattcc
    19621 ttagatgatg caaaagttaa ccttacaccg gtcatcgatt ctggagactt ttttaaaatc
    19681 cccatatatt aatgatgtaa gaatactgga ttggtgtttt aataaaagaa tgcaatattt
    19741 tggtgattct gaaaagataa agtatgcatg ctccgtgata aatcatattg attttagccg
    19801 cgatcaatct aaagatttta gctgcgatca atctaaaatt aaaatagctg agaccttatt
    19861 tttcaatctc gataaagaac cctataaaaa
//
LOCUS       DADLTD010000061        19875 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLTD010000061 DADLTD010000000
VERSION     DADLTD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.061"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            54..830
                     /locus_tag="JN701_001741"
     CDS             54..830
                     /locus_tag="JN701_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY7364214.1"
                     /translation="MTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTG
                     RAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDM
                     GRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVG
                     TPFFVRYGRVKIEDQIGEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEAD
                     RTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            840..1499
                     /gene="eutL"
                     /locus_tag="JN701_001742"
     CDS             840..1499
                     /gene="eutL"
                     /locus_tag="JN701_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY7364215.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1512..2012
                     /gene="eutK"
                     /locus_tag="JN701_001743"
     CDS             1512..2012
                     /gene="eutK"
                     /locus_tag="JN701_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY7364216.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2058..3110
                     /gene="eutR"
                     /locus_tag="JN701_001744"
     CDS             2058..3110
                     /gene="eutR"
                     /locus_tag="JN701_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY7364217.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="JN701_001745"
     CDS             complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="JN701_001745"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY7364218.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="JN701_001746"
     CDS             complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="JN701_001746"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY7364219.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5102..5527
                     /locus_tag="JN701_001747"
     CDS             5102..5527
                     /locus_tag="JN701_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY7364220.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5514..5963
                     /locus_tag="JN701_001748"
     CDS             5514..5963
                     /locus_tag="JN701_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY7364221.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6170..6745
                     /locus_tag="JN701_001749"
     CDS             6170..6745
                     /locus_tag="JN701_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY7364222.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6841..7740
                     /gene="yfeX"
                     /locus_tag="JN701_001750"
     CDS             6841..7740
                     /gene="yfeX"
                     /locus_tag="JN701_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY7364223.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="JN701_001751"
                     /pseudo
     CDS             complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="JN701_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9107..10531)
                     /gene="murP"
                     /locus_tag="JN701_001752"
     CDS             complement(9107..10531)
                     /gene="murP"
                     /locus_tag="JN701_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY7364224.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="JN701_001753"
     CDS             complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="JN701_001753"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY7364225.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11595..12452
                     /gene="murR"
                     /locus_tag="JN701_001754"
     CDS             11595..12452
                     /gene="murR"
                     /locus_tag="JN701_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY7364226.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12581..13372
                     /gene="ucpA"
                     /locus_tag="JN701_001755"
     CDS             12581..13372
                     /gene="ucpA"
                     /locus_tag="JN701_001755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY7364227.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13676..14692
                     /locus_tag="JN701_001756"
     CDS             13676..14692
                     /locus_tag="JN701_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7364228.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14692..15525
                     /gene="cysT"
                     /locus_tag="JN701_001757"
     CDS             14692..15525
                     /gene="cysT"
                     /locus_tag="JN701_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY7364229.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15525..16400
                     /gene="cysW"
                     /locus_tag="JN701_001758"
     CDS             15525..16400
                     /gene="cysW"
                     /locus_tag="JN701_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY7364230.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16390..17487
                     /gene="cysA"
                     /locus_tag="JN701_001759"
     CDS             16390..17487
                     /gene="cysA"
                     /locus_tag="JN701_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY7364231.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17621..18532
                     /gene="cysM"
                     /locus_tag="JN701_001760"
     CDS             17621..18532
                     /gene="cysM"
                     /locus_tag="JN701_001760"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY7364232.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18535..18903)
                     /locus_tag="JN701_001761"
     CDS             complement(18535..18903)
                     /locus_tag="JN701_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY7364233.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            19008..19859
                     /gene="pdxK"
                     /locus_tag="JN701_001762"
                     /gene_synonym="thiJ"
     CDS             19008..19859
                     /gene="pdxK"
                     /locus_tag="JN701_001762"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY7364234.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4572 a         5044 c         5610 g         4649 t
ORIGIN      
        1 cggcccccgc gctgtcagaa gcaaagtgca ccaccaccac ctgtgcggca ccggtgacct
       61 cggaaagctg cgcgctggat ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa
      121 acccgcatcg cgcagacgta ttaacagaac tgcgtcgcag caccgtggcc cgcgtttgta
      181 ccggtcgtgc cggtccgcgt ccgcgtaccc aggcgttgct gcgtttcctg gccgatcact
      241 ctcgttcgaa agataccgta ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct
      301 tgctggaagt acgctccgaa attagcgaca aaaatctcta cctgactcgc ccggatatgg
      361 gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg
      421 acgtacaggt cgttatttct gatggcctgt caactgatgc catcaccgtg aactacgaag
      481 agatcctgcc gccgctgatg gcgggcctga aacaggccgg gctgaaagtc ggcacgccat
      541 tctttgttcg ttatggtcgc gtgaagattg aagatcagat cggtgagatc ctcggcgcga
      601 aagtggtgat cctgctggtg ggggaacgtc cggggttggg tcagtcagaa agcctctcct
      661 gttacgccgt ttactcgccg cgtatggcga ccaccgttga ggccgatcgc acctgcatct
      721 ctaacattca ccagggcggc acgccgccgg ttgaagccgc agccgtcatt gtggatttgg
      781 cgaaacgtat gctggagcag aaagcatccg gcatcaacat gacccgataa ggaggcatca
      841 tgccagcttt agatttgatt cgaccgtcgg taaccgccat gcgggtgatt gcctccgtta
      901 acgctgaatt tgcgcgtgaa ctaaaattgc cgccgcatat tcgtagcctc gggctgattt
      961 ctgctgattc cgatgacgtc acctacattg cggctgacga agcgaccaag caggcgatgg
     1021 tcgaagtggt atatggccgc tcgctgtatg cgggagcagc acacggaccg tcaccgactg
     1081 ccggtgaagt gctgattatg cttggtgggc caaacccggc ggaagtgcgt gctggtctgg
     1141 atgcgatggt tgcgcatatt gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa
     1201 acaccgcatt cctggcacat gtagtttcgc gtaccggttc ttatctctca tcaaccgccg
     1261 ggatcacgct tggcgatccg atggcgtatc tggtggcacc gccgctggaa gcgacctacg
     1321 gcattgatgc agcgttgaaa tctgccgacg tacagctggt gacctatgtc ccgccaccgt
     1381 ctgaaaccaa ctactcggca gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct
     1441 gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac
     1501 ggaggttgcc gatgatcaat gcactgggat tgctggaagt ggacggcatg gtcgccgcga
     1561 tagatgcagc ggatgccatg ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc
     1621 ttgaccctgg tcgcttaacg ctggtggtgg aaggcgatct ggcggcgggt cgtgcggcgc
     1681 tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg
     1741 gtcggccaga cgatgacacc cagtggctgg ttactggctt taaccgccag ccgaagcaac
     1801 ccgtaaggga acccgacgcg ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc
     1861 tgttaacatc agtacgtcag ggaatgacgg caggagaagt ggctgcccac tttggctggc
     1921 cgctggaaaa agccagaaat gcgctcgaac agctcttttc tgccgggacg ttacgtaaac
     1981 gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg aggtgctggg tgtcctacaa
     2041 cacccggcat taacatcatg aaaaagaccc gtacagccaa tttgcaccat ctttatcatg
     2101 aacccttacc cgaaaacctg aagctcacgc cgaaggtcga agtggataat gttcatcaac
     2161 gacagacaac ggatgtctat gaacatgctt taacgattac cgcctggcag cagatttacg
     2221 atcagctgca tccgggcaag tttcatggtg aatttacgga aattctactc gatgatattc
     2281 aggtttttcg tgaatacacc ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact
     2341 cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc
     2401 tgggaagcgc ggaaatcgcc acccgccctg gtggcactga atttgaactg agcacgccgg
     2461 atgattacac gatcctgggc gtggtgcttt ctgaagatgt catcacccgg caggctaact
     2521 ttttgcataa cccggatcgg gtattacata tgctgcgtag ccagtcggcg ctggaagtga
     2581 aagagcagca taaagccgcg ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg
     2641 agaacccgga aaatctccat cagcctgcag tgcgaaaagt gctgggggat aatttgctaa
     2701 tggcgatggg ggcgatgctg gaagacgcgc aaccaatggt gacggcggaa agcatcagtc
     2761 atcagagtta ccgtagatta ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg
     2821 agccggtgac ggtgctggac ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa
     2881 acgcgtttca cgctatttta ggcattggcc caaacgcgtg gctgaaacgc attcgcctga
     2941 acgccgtacg ccgcgaactg ataagcccgt ggtcgcaaag cacaacggta aaagacgccg
     3001 ccatgcagtg gggattctgg catctggggc aatttgtcac ggattaccag cagctgtttg
     3061 ccgagaagcc gtcgttgacg ttgcatcagc ggatgcggga atgggggtga gggagttaca
     3121 cccaatccct gaccttaata aactcactta acgccgcttc tgggctgcca tcttttggct
     3181 gataatcata ctcccagcgt accagtggcg gcattgacat caggatcgac tcggtgcgcc
     3241 cgccagtttg caggccaaac agagtgccgc gatcccagac cagattgaac tcgacataac
     3301 gaccgcgacg gtagagctga aaattgcgct cgcgctcgcc gtaggccatt gctttacgtc
     3361 gctctacaat tggtaaataa gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa
     3421 aacagtggtc gaaatctggc gtgttcagat catcaaagaa cagcccgcca ataccgcgct
     3481 gttcgttgcg atgtttgagg tagaagtaat cgtcgcacca ctttttgtaa cggggataaa
     3541 cgtcttcacc aaatggcagg cacaggtcac gggcggtgcg gtgccagtga atggcgtctt
     3601 cttcaaaacc atagaaaggg gttaaatcga agccgccgcc aaaccaccag acgggctctg
     3661 cacccggttt ttcggcaata aaaaaacgca cattcgcgtg gctggtggga acatacgggt
     3721 tatgcggatg cactaccagt gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg
     3781 ggcgatgagc ggtggcggaa gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc
     3841 ctgcctgttc gaaaacacca ccattacgca acacccgact acgcccgccg ccgccagctt
     3901 cgcgctgcca actatcttcg acaaattctg cgccatcgac ggcggtcagc tgctgacaaa
     3961 tcgtatcctg aaggttgagc agaaactgtt taacctggtg tgcgtcgggt ttcataactt
     4021 atcgcttttt cgaatgtgct ttctggttgt cgaaccagtg gaaataactg atcacgcctt
     4081 cagcaatcgc tgtggcgatt ttctgacgaa acgccgccgt gcctaacagc cgctcttctt
     4141 ccgggttggt gataaacgag gtttccacca gcaccgaagg aaccgacggc gatttcaaca
     4201 ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt
     4261 tcagaatatg cgagccaagc gtcaggctgt ttttaatagt atcggtttgc accagatcaa
     4321 acagcacttg ctgcaatagg tgatccttgt cagtcgcctt tttaccggca acttcatcgg
     4381 cgcggttttc gcgttcagac aggtatttcg ccattgcgct acttgcccca cggttagaga
     4441 gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa gccatcggca tgaattgaca
     4501 taaacagatc tgcgccatgt ttatgggcga tttcaacgcg atcgtaaagt gggataaacg
     4561 tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt
     4621 ttttagcaat cgccagcacc acatgttttt ctttcgaacc gttgcgtccg atcgctccgg
     4681 tatcaattcc gccgtggcct ggatcgagaa caacgacacg tttgccgcca gattttttgg
     4741 ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc gtctttggcg atggcttgcg
     4801 acattcctga caacgtcagg gcagccaaac cggctttcag cacctggcgg cgcgaagtga
     4861 gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt gtaataataa agttccagat
     4921 gttatatcgt atcgcgtaat ccgttacgac ggtttgatgt tgagaattgt tttacttttc
     4981 atttcaatag ttgacaactc cccattatgc cactttttca accgattttc gtcagatatt
     5041 ggctaattct accgttcacg ccataatcag ccttttaacc cgcaaaaagg taacgaatac
     5101 catggagata cgcgtatttc gccaggaaga tttcgaagag gtcatcaccc tttgggagcg
     5161 ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac atcgagcgta agatgaacca
     5221 tgacgtcagt ttgtttttgg tcgctgaggt gaacggtgaa gtggtcggaa cggtgatggg
     5281 cggttatgac gggcatcgcg ggtctgctta ttatcttggc gtgcatccag agtttcgtgg
     5341 gcgtgggatt gccaatgcgt tgcttaatcg gctggagaaa aagctgattg ctcgtggctg
     5401 cccgaaaatt cagatcaatg tgccggaaga taacgacatg gtgctcggaa tgtatgaacg
     5461 cctgggatat gaacacgccg acatgctgag tctgggtaag cgtttgattg aagatgaaga
     5521 gtactgagtt ccatcctgtc cattatgatg cgcacggacg cctgcgttta cccttgctct
     5581 tctggcttgt gctattgctt caggcgcgaa cctgggtgct gtttatcatt gccggtgcgt
     5641 cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc cgatcacgat aatttctggc
     5701 tggggttgat tcccggtatt ccggcggtgc tggcgttttt gttgagcggt cggcgggctt
     5761 cgtttcctcg cacctggcat gtgctctatt ttctgttgct gttggcgcag gtggttttac
     5821 tttgctggca accgtggctg tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc
     5881 tggtagtggc ggatattgtg gcgttaatct ggctgctgac caatcgacgt ttacgcgctt
     5941 gttttaatga agaaaaagaa taacgcagag gtgcgctttg tttatgccgg atgcggcgtg
     6001 aacgccttat ccggcctaca aaacatcgaa aattcaatat attgcaggag aaagtaggcc
     6061 tgataagcgt agcgcatcag gcaattttgt gtttgtcatc aacctttaac ggcacttttt
     6121 ggtgaatttg cactccaagc aacgttattg aataaccaaa ggaagtgaca tgaaatcgct
     6181 gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc tgttccacga tgtcgtcagt
     6241 taactggtcg gctgctaacc cgtggaactg gtttggatcg tccaccaaag tgagcgagca
     6301 gggtgtgggt gaattaacgg cgtccacacc actgcaagaa caagccattg ctgatgcgct
     6361 tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt
     6421 ttttgaagtg atgaaaggcg acaacgtggc gatggtgatt aacggcgatc aaggcacgat
     6481 cagtcgcatt gacgtgctgg atagcgatat tcctgctgac accggtgtta aaatcggtac
     6541 accgtttagc gacctttaca gcaaagcatt tggcaattgc caaaaagctg atggtgatga
     6601 taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat attagctacc agttcagagg
     6661 ggaatggcgt ggtcctgaag ggttaatgcc ttcggacgat accctgaaaa actggaaagt
     6721 cagtaaaatt atctggcggc gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca
     6781 cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat tcttattttc aggaggaaca
     6841 atgtctcagg ttcagagtgg cattttgcca gaacattgcc gcgcggcgat ttggatcgaa
     6901 gccaacgtga aaggggaagt tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa
     6961 ctggcaactt ttgaagcgaa attcccggac gcgcatcttg gtgcggtggt tgcctttggt
     7021 aacaacacct ggcgcgctct gagcggcggc gttggggcag aagagctgaa agattttccg
     7081 ggctacggta aaggccttgc gccgacgacc cagttcgatg tgttgatcca cattctttct
     7141 ctgcgtcacg acgtaaactt ctctgtcgct caggcggcga tggaagcctt tggtgactgc
     7201 attgaagtga aagaagagat ccacggcttc cgttgggtgg aagagcgtga cctgagcggc
     7261 tttgttgacg gtacagaaaa cccggcgggt gaagagacgc gccgcgaagt ggcggttatc
     7321 aaagacggcg tggatgccgg cggcagctat gtgtttgtgc agcgttggga gcacaacctg
     7381 aagcagctca accggatgag cgttcacgat caggagatga tgatcgggcg caccaaagag
     7441 gccaacgaag agattgacgg cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat
     7501 ctgaaagaag atggcaaagg gctgaagatt gttcgccaga gcctgccgta cggcaccgcc
     7561 agtggcactc acggtctgta cttctgcgcc tactgcgcgc gtctgcataa cattgagcag
     7621 caactgctga gcatgtttgg cgataccgat ggtaagcgtg atgcgatgtt gcgcttcacc
     7681 aaaccggtaa ccggcggcta ttacttcgcg ccgtcgctgg acaagctgat ggcgctgtaa
     7741 gcgtttctga ttctatgatt aaaggccagc cagtaacaaa actgactggc ctttttatta
     7801 cttttgcttc aacgccgcat acacctgatc aactacccaa ccataagttg caatcggtaa
     7861 ctgaccgctt tcgaacatat tgggattttt ttgtggatcg gcaactggcg aatggggctt
     7921 gttgcttaac atcacaatcg ccatatgatt gaccggatcg ataacggtta ctgtcccggt
     7981 ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt
     8041 ggcattacca ttcacgcgcc agccgaggcc aaaagtggca tcttccttag agctggtggt
     8101 gaacatcttc accgtttccg cactgaacag ctgcacatcg ccatagcccc cgccgttcag
     8161 catcgtttgc attaacaccg caatatcgcc ggtattggaa aacaaacctg cgtgcccgga
     8221 aacgccgccc atcgaataaa aggctttttc atcgtgcacc tgaccccaga gagtggaggt
     8281 gcggatattc gggaaatgga tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc
     8341 aatttgttgc ggtttaaagc ctttcagcag cgggttaaat accgtatgtg tcaggccgag
     8401 cgggcgataa atcgactctt caacatagcg gtcaagcggc tgaccggtaa ccgactcgac
     8461 gataaatcca agcagcatat aatcgacatc gctgtagata tgtttgctgc cgggctgata
     8521 ttccagcggc gtgcgcttga tcatctccag cgtctggcct ttatcctagg aatataacgc
     8581 gcccgcgacc gctttattcg ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag
     8641 caggtcagaa atccgcaggg tgtttttgcc tttaatggtg tcatttggac tatcggcaaa
     8701 ccccggaata tatttcgcaa tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt
     8761 ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag gccaggtcat acagcgtccc
     8821 ggtggtggct ttgacaggct gttccatcaa cacgctgcca tcgtactttt tcgccgctcc
     8881 ccaggctttg cgatacacaa tctgattatc tttaatgatc agcaggttta cgccgggata
     8941 accggcatca acttgctggc taatccagcg atccatctgg ttaagccgtt tgacgttaaa
     9001 ccctgctttc tctggcgagc tttctgtcag aacagggtat tttgcggcgc tgacgctaca
     9061 actaacagcc agcagagaaa gatagagcat tgtccgtttc atgatatcag tccagattga
     9121 cgttacggca gccaaagagc gtggtgaaaa taaacccgca aacccatgcc accagaatcc
     9181 cgccagcata aaccgccatg gccggaagga tgccttgtgc ggaagtcatc agcggcagcg
     9241 ctaccagacc agacggccca aaggcgctgt ttaagcccat cggcagaccc caccaggcta
     9301 tcaggccgat aaacaaaccg cccgccgcac cgcctaaaca ggcggtgaca aacggcttca
     9361 tgcggggcag ggtgacaccg taaatcagcg gttcaccaac gcccagcagg ccgggaataa
     9421 tcgccccgcg tacctgactg cgtagcgcac tgtgcggttg cgaccgccag tagagtgcca
     9481 gcgccgcgcc cacctggccc gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc
     9541 cctggctgtc cattaacgcg aggtaaacag gaataaagcc ctgatgcacg ccaaatacca
     9601 cggcgatcag gaacagcccg gctaaaaccg cacaaccgaa cggattactg ttcaggtgca
     9661 taaacagcca tgacatacct tcgaataacc agccacccag cggcataatg atcaggtacg
     9721 caagcgtagc ggtgatcagc agggtgatta acgaggtcag cagcatgtcg agatcgtccg
     9781 gcataaagcg gcgcaccatg ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc
     9841 cgataatatt gccgcgcgga tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt
     9901 aaccggtggt tgcagcgggg ttataaccga gcaaaaagag tgcggcaata attgcgccat
     9961 tgacgcccgt gccgccaaat gcctgggcgg cgttatagcc caccagaatc accaggaagg
    10021 tgaacaaacc tttgctgaac accttcataa aattcagcgc gtcgggtagc gtgccctgag
    10081 catctgccgg aacgtgcatc accgtggcaa ttaacgtcgc tatccccagc agcagaccgg
    10141 cggcaataaa accggggatc agcggcgtga agatggtggc gaatttggcg agaaattgtt
    10201 gcacgccgga ggtttgtttg gcttttaact gacgtttgtt ctgggcggcg atttctgcgg
    10261 catcctgtac cggtgcctcg cccagcagct cgctcatcgc ttttgcggca cgatgcgctt
    10321 taccaggtcc aaaaacgacc tgcacctggt cgctggtcaa aatgacgctc ttcacgcctt
    10381 ccagcgtttt gatattggga tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg
    10441 tcatacagtt accacaactg gcgatatttc ccggtccgcc gacacgggta agaatggtgt
    10501 tcagaagttc actgctgatc tctttggcca tgggttattc cttgtctaaa acctgacgaa
    10561 taaagccgcc gtgttgatcc aggcgttttt tagcttctgc ggcatcgaga tttttcagca
    10621 ccatcacaat ggccgtttta cagttgcgct cgcaggcaat taacgccgct tccgcttgtt
    10681 cggcattaca tccggtggcg tttttaacga tattgacctg gcgcacatgc agtttttcgt
    10741 tggtggcgac cacatcgacc ataaggttgc cgaacacttt gccggattta atcatcagcc
    10801 cggtggaaag catattgagc accagtttct gcgctgtacc tgctttcatc cgcgaagaac
    10861 cggtaacaac ttcggcacct acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg
    10921 cgctccccgg attacaggaa attcccactg tgcggcagcc gagctggcgt gcgtattcca
    10981 gtccggcaat cacatacggc gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg
    11041 ccgttaaatt aatatttttc agatcattaa caccgccttc ccggctatct tccgcgcctt
    11101 ccaccgcgtg ctgaatggca tattcgccgc cagcaatcaa accaaccacc agacccggtt
    11161 tcacgccgta ggtgggcgga cattcgctgg catccagaat ccccagacga ccggatgttc
    11221 ccgcaccgag gtaaatcaga cgcccgccgc cgcttacctg ggcgtggata acatcgatcg
    11281 ccgcggcgat atccggcagt acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt
    11341 tgataatccg gcacatttcc agcgttgata cgcggtcaat ttcagccgag gcggcgttcg
    11401 agccttcagt aatcatcttt tcaagttgca tcggtggtga ccttactatt tacgatcaaa
    11461 taattgcaga tgtatggaat catatatcct gaattgacag acttaaagga atcattgagt
    11521 ctacactcgc agagtgtgaa gagcgtcata agaacttaag cggaatttat gctgccagcc
    11581 caggagagtg gaagatgttg tacctgacga aaataagcaa tgcggggagt gaattcaccg
    11641 aaaacgagca aaaaatcgct gactttctgc aggccaacgt gagtgagctg caatccgttt
    11701 cttcacggca gatggcgaaa cagctcggca ttagccagtc gagcatagtg aaatttgccc
    11761 aaaaactcgg cgcgcaagga tttactgaat tgcgtatggc gttaataggc gaatacagtg
    11821 ccagccggga gaaaacgaat accacggcgc tgcacctgca cagttcgatt accagtgatg
    11881 attcgctgga agtgattgcc cgcaaattga atcgcgaaaa agagctggcg ctggagcaaa
    11941 cctgtgcgtt gttcgactac gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac
    12001 cgtttatcca gataacgggc ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca
    12061 aactgatgaa aattggctat cgcgtggcct gcgaagccga cacgcacgta caagccaccg
    12121 tttcccaggc gttgaaaaaa ggcgatgtac aaatcgctat ctcttacagc ggtagtaaaa
    12181 aagagattgt cttgtgcgta gaagcggcac gaaagcaggg ggcaacggtg attgccatta
    12241 cttctctggc tgactctccg ctgcggcggc tggcgcattt cacgctcgat accgtttccg
    12301 gtgaaaccga atggcgtagc tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg
    12361 atttactgtt tgttggtctt gtgcaactca atgatgtggc gtcattgaaa atgattcagc
    12421 gcagcagtga gctaacacaa cgcctgaaat gatgcataaa gcagcgactg gattgagatt
    12481 ttcctgaatt agtgagctga tccgcagcaa tattttgttt atcctgtatt ttcagaggga
    12541 atggagtgta acgctctgta ttaacaagga gagcattaaa atgggtaaac tcacgggcaa
    12601 gacagcactg attacgggcg cattgcaggg aattggcgaa ggaattgcca gaacttttgc
    12661 acgtcatggc gcgaacctaa tcttgctgga tatctcccct gagatcgaaa agctggcgga
    12721 cgaactgtgt ggtcgtggtc atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc
    12781 gtcggtagcc gcagctatca aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt
    12841 gaataacgca ggcgtttgtc gtctgggcag tttcctcgat atgagcgatg acgatcgcga
    12901 tttccatatt gatatcaata ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga
    12961 gatgattgcg cgcaaagatg gtcgcattgt gatgatgtct tcagtcactg gtgatatggt
    13021 ggccgatcct ggcgaaacgg cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa
    13081 atcgctggcg gtggagtacg cgcagtctgg tattcgcgtt aacgccattt gcccgggata
    13141 cgtgcgcaca ccaatggcgg aaagcattgc ccgccagtcg aacccggaag atccagagtc
    13201 ggtgctgact gaaatggcga aagcaatccc gatgcgtcgc ctcgccgatc cgctggaagt
    13261 cggcgaactg gcggccttcc tcgcatcgga tgaatccagc tatttaaccg gtacacagaa
    13321 tgtgattgat ggcggcagca cactgccgga gacggttagc gtcggtatct gattcacctc
    13381 tgtttcctcc ctgcatgtgt ggggaggatt tcgtcttgaa ctaagttcac caggctattt
    13441 tatttgtcat tttggccccg ggcagtgctc gaaatactca cgtactatgt gtacgctccg
    13501 gtttctccgc gctgtccgtg tccaaactgc ctgcaacaat tacgcctgtt gaaccaagtt
    13561 cttattccct ttttaacttc caaatcacca aacggtatat aaaaccgtta ctcctttcac
    13621 gtccgttata aatatgatgg ctattagaaa gtcattaaat ttataagggt gcgcaatggc
    13681 cgttaactta ctgaaaaaga actcactcgc gctggtcgct tctctgctgc tggcgggcca
    13741 tgtacaggca acggaactgc tgaacagttc ttatgacgtc tcccgcgagc tgtttgccgc
    13801 cctgaatccg ccgtttgaac agcaatgggc aaaagataac ggcggcgaca aactgacgat
    13861 aaaacaatct catgccgggt catcaaaaca ggcgctggca attttgcagg gcttaaaaac
    13921 cgacgttgtc acttataacc aggtgaccga cgtacaaatt ctgcacgaca aaggcaagct
    13981 gatcccggct gactggcagt cgcgcctgcc gaataatagc tcgccgttct actccaccat
    14041 gggcttcctg gtgcgtaagg gcaacccgaa gaatatccac gactggaacg acctggtgcg
    14101 ctccgacgtg aagctgattt tcccgaaccc gaaaacgtcg ggtaacgcgc gttataccta
    14161 tctggcggca tggggcgcag cggataaagc tgacggtggc gacaaagcca aaaccgaaca
    14221 gtttatgacc cagttcctga aaaacgttga agtgttcgat actggcggtc gtggcgcgac
    14281 caccactttt gccgagcgcg gcctgggcga tgtactgatc agcttcgagt cggaagtgaa
    14341 caacatccgt aaacagtatg aagcgcaagg ctttgaagtg gtgattccga aaaccaacat
    14401 tctggcggaa ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg gtacggaaaa
    14461 agcagcaaaa gcctacctga actggctcta cagcccgcag gcgcagacca tcatcaccga
    14521 ctattactac cgcgtaaata acccggaagt catggacaaa ctgaaagata aattcccgca
    14581 gaccgagctg ttccgcgtgg aagacaaatt tggctcctgg ccggaagtga tgaaaaccca
    14641 cttcaccagc ggcggcgagt tagacaagct gttagcggcg gggcgtaact gatgtttgca
    14701 gtttcatcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt
    14761 gtgtgcctga ttttgctgct gccgctctcc gcgctggtga tgcagctggc tcagatgagc
    14821 tgggcgcagt actgggaggt gatcaccaac ccacaagtgg tcgcggccta caaagtgacg
    14881 ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg
    14941 attttaacac gttatcgctt tccgggccgc acgctgcttg atgcgctgat ggatttaccc
    15001 tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc cgtaaacggt
    15061 ttttacggtg aatggctggc gaagtttgat atcaaagtca cctatacctg gcttggtatt
    15121 gcggtggcga tggcctttac cagcattccg tttgtggtgc gtaccgtgca gccggtgctg
    15181 gaagagttag gcccagaata tgaagaagcg gcggaaacgc ttggtgcaac gcgctggcag
    15241 agtttctgca aagtggtgct gccggaactt tctccagcgc tggtggcggg cgtggcgttg
    15301 tcgtttaccc gtagtcttgg tgagtttggc gcggtgatat ttatcgccgg gaacatcgct
    15361 tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt tgattacccg
    15421 gcggcgagcg cgattgcttc ggtgatcctc gcggcatccc tgctgctgct gttctcaatt
    15481 aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagttaccc
    15541 aattgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg
    15601 ggatgctggt atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt
    15661 tcagcaaagg gctgatgccg gttttacaga atctggccga tccggacatg ctgcacgcca
    15721 tttggctgac ggtgatgatt gcgctgattg ccgtaccggt gaacctggtg ttcggcattc
    15781 tgctggcgtg gctggtaacg cgctttaact tccccggacg ccagttactg ctgacgctac
    15841 tggacattcc gtttgccgta tcgccggtgg ttgccggtct ggtgtatctg ctgttctacg
    15901 gctctaacgg tccgctgggc ggctggctgg atgaacataa tttgcagatt atgttctcct
    15961 ggccgggaat ggtgctggtc accatctttg tgacctgtcc ttttgtggtg cgcgagctgg
    16021 tgccggtgat gttaagccag ggcagccagg aagacgaagc ggcgattttg cttggcgcgt
    16081 ctggctggca gatgttccgt cgcgtcacat tgccgaacat tcgctgggcg ctgctgtatg
    16141 gtgtggtgct aaccaacgcc cgcgcgattg gcgagtttgg tgcggtgtcg gtggtttccg
    16201 gctcgattcg cggcgaaaca ctttcgctgc cgttacagat tgaattgctg gagcaggact
    16261 acaacaccgt cggctccttt accgccgccg cgctgttgac gctgatggcg attatcaccc
    16321 tgtttttaaa gagtatgttg cagtggcgtc tggagaatca ggaaaaacgc gcgcagcagg
    16381 aggagcatca tgagcattga gattgccaat attaagaagt cgtttggtcg cacccaggtg
    16441 ctgaacgata tctcactgga tattccttct ggtcagatgg tcgcgctgct ggggccgtcc
    16501 ggttccggaa aaaccacgct gctgcgcatt attgccgggc tggagcatca aaccagcggg
    16561 catattcgct tccacggtac cgacgtgagc cgcctgcacg cacgcgatcg taaagtcggt
    16621 ttcgtgttcc agcattacgc gctgttccgt catatgacgg tgttcgacaa tatcgctttt
    16681 ggcctgacgg tgctgccacg tcgcgagcgc ccgaatgccg cagccatcaa agcgaaagtg
    16741 acaaaattgc tggagatggt ccagcttgcg catctggcgg atcgttatcc ggcacagctt
    16801 tccggcggcc agaaacagcg cgtggcgctg gcgcgcgcgc tggctgttga accgcaaatt
    16861 ctcctgcttg atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga gctgcgtcgc
    16921 tggctgcgtc aactgcatga agagctgaaa ttcaccagcg tgttcgtgac ccacgaccag
    16981 gaagaagcga ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa tatcgagcag
    17041 gctgatgcgc cggaccaggt atggcgcgaa ccagcgaccc gatttgtgct cgaatttatg
    17101 ggcgaagtga accgcctgca gggaaccatt cgcggcgggc agttccatgt tggcgcacat
    17161 cgctggccgc tggggtatac gcctgcgtat caggggccgg tggatctctt cctgcgcccg
    17221 tgggaagtgg atatcagccg ccgtaccagt ctcgattcgc cgctgccggt acaggtactg
    17281 gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc agccgctggg gtggtacaac
    17341 gaaccgctga cggtcgtgat gcatggcgac gatgccccgc agcgtggcga gcgtttattc
    17401 gttggtctgc aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac ccgagatgag
    17461 gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc ccggaggcgc
    17521 ttcccgcgat ccgggctttt taatggcaag gtttgtaacc tgtagacctg ataagacgcg
    17581 caagcgtcgc atcaggcaac accacgtatg gatagagatc gtgagtacat tagaacaaac
    17641 aataggcaat acgcctctgg taaagttgca gcgaatgggg ccgaataacg gcagtgaagt
    17701 ctggttgaaa ctggaaggca ataacccggc aggttcggtg aaagatcgtg cggcactttc
    17761 gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt taatcgaagc
    17821 caccagtggt aacaccggca ttgcgctggc aatgattgcc gcgcttaaag gctatcgcat
    17881 gaaattgctg atgcccgaca acatgagcca ggaacgccgt gcggcgatgc gtgcttatgg
    17941 tgcggaactg attcttgtca ccaaagagca gggcatggaa ggtgcgcgcg atctggcgct
    18001 ggagatggcg aatcgtggcg aaggaaagct gctcgatcag ttcaataatc ccgataaccc
    18061 ttacgcccat tacaccacta ccgggccgga aatctggcag caaaccggcg ggcgcattac
    18121 ccattttgtc tccagcatgg gaacaaccgg cactatcacc ggcgtttcac gctttatgcg
    18181 cgaacaatcc aaaccggtga ccattgtcgg cctgcagccg gaagaaggca gcagtattcc
    18241 cggcattcgc cgctggcctg cggaatatct gccggggatt ttcaacgctt ctctggtgga
    18301 tgaggtgctg gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac tggcggtgcg
    18361 ggaaggaata ttctgtggcg tcagctcagg cggcgcggtt gctggagcac tgcgggtggc
    18421 aaaagctaac cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg atcgctacct
    18481 ctccaccgga gtgtttgggg aagagcattt tagccagggg gcggggattt aagattattg
    18541 cgcaggcacc gttttatccg tctgcgcaat tagcgcatgt aaaaaaggct gtgcgttctc
    18601 atcgctctta ccggggatct tcacaatata cggcttcccg gtaatcgatg acgacgaagc
    18661 aaccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc cgagcttcag
    18721 acgcagatga gaaaccgctt catcgcaggt atgttcatca ccgttgcgca caaatatcaa
    18781 atcctttttc tgtgataatc cctccagcat ggcgttgatg cgggcttctt catgggcagt
    18841 taacttcgcg taagcgggga gtgtcattag tagtgtaatg accagacaaa tgattttctt
    18901 cattgaatta ccttatcctt gttttgtaat agccgatttg actgccagaa tcaggtaaag
    18961 tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg agtagtttgt
    19021 tgttgtttaa cgataagagc agggcgctgc aggcggatat cgtcgccgtg cagtcgcagg
    19081 tggtttacgg cagcgtgggc aacagcattg ccgtgcctgc tatcaaacag aacggtctga
    19141 atgtttttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac actttctacg
    19201 gtggtgcgat tccggatgaa tggtttagcg gttatttgcg tgcgcttcag gagcgtgatg
    19261 cgctgcgcca gctgcgcgct gtaaccacgg gctatatggg aacggcatcg caaatcaaaa
    19321 tccttgccga gtggctgact gcgctacgca aagaccatcc tgacctattg atcatggtcg
    19381 atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt cccgaggcgt
    19441 atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt gagctggaaa
    19501 tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca aaaagtctgc
    19561 tttcagacac attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa gaaaatcagg
    19621 agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt catttcccat tcacgggtaa
    19681 aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt ggtttgctga
    19741 aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg gaagtgatgc
    19801 gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg gaagcataaa
    19861 aaatggcgcc gatgg
//
LOCUS       DADLTD010000062        19593 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLTD010000062 DADLTD010000000
VERSION     DADLTD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.062"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(188..529)
                     /locus_tag="JN701_001763"
     CDS             complement(188..529)
                     /locus_tag="JN701_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7364235.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(532..4311)
                     /gene="tamB"
                     /locus_tag="JN701_001764"
     CDS             complement(532..4311)
                     /gene="tamB"
                     /locus_tag="JN701_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY7364236.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4308..6041)
                     /gene="tamA"
                     /locus_tag="JN701_001765"
     CDS             complement(4308..6041)
                     /gene="tamA"
                     /locus_tag="JN701_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY7364237.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6247..6885
                     /gene="msrA"
                     /locus_tag="JN701_001766"
     CDS             6247..6885
                     /gene="msrA"
                     /locus_tag="JN701_001766"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY7364238.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7208..8551
                     /locus_tag="JN701_001767"
     CDS             7208..8551
                     /locus_tag="JN701_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7364239.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8613..8819)
                     /locus_tag="JN701_001768"
     CDS             complement(8613..8819)
                     /locus_tag="JN701_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY7364240.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9144..9698
                     /locus_tag="JN701_001769"
     CDS             9144..9698
                     /locus_tag="JN701_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7364241.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(9788..10699)
                     /locus_tag="JN701_001770"
     CDS             complement(9788..10699)
                     /locus_tag="JN701_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364242.1"
                     /translation="MLPRIRHNNFIGAVVLFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKDYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLALPV"
     gene            complement(10911..11651)
                     /gene="cysQ"
                     /locus_tag="JN701_001771"
     CDS             complement(10911..11651)
                     /gene="cysQ"
                     /locus_tag="JN701_001771"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY7364243.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            11841..13784
                     /gene="cpdB"
                     /locus_tag="JN701_001772"
     CDS             11841..13784
                     /gene="cpdB"
                     /locus_tag="JN701_001772"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY7364244.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(13913..14293)
                     /locus_tag="JN701_001773"
     CDS             complement(13913..14293)
                     /locus_tag="JN701_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7364245.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSITPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            14382..15242
                     /gene="qorB"
                     /locus_tag="JN701_001774"
     CDS             14382..15242
                     /gene="qorB"
                     /locus_tag="JN701_001774"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7364246.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            15350..16315
                     /locus_tag="JN701_001775"
     CDS             15350..16315
                     /locus_tag="JN701_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7364247.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            16423..17085
                     /gene="ytfE"
                     /locus_tag="JN701_001776"
     CDS             16423..17085
                     /gene="ytfE"
                     /locus_tag="JN701_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY7364248.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(17130..18542)
                     /gene="cycA"
                     /locus_tag="JN701_001777"
                     /gene_synonym="dagA"
     CDS             complement(17130..18542)
                     /gene="cycA"
                     /locus_tag="JN701_001777"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY7364249.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(18851..19471)
                     /locus_tag="JN701_001778"
     CDS             complement(18851..19471)
                     /locus_tag="JN701_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7364250.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         4801 a         5189 c         4771 g         4832 t
ORIGIN      
        1 tactcaccgt tcaaagcgtg cccccgtttt acccctgcac atttgtgaac gccagcagag
       61 ttttttatgc gattgcagat atggaaagcg gctcggagag aaaagcaaaa gatggaaaag
      121 ttacaaaaaa aaatcgcgag atacatcgca taaaacaacg ccacccgaag gtggcgtgct
      181 tttattatta cttatccctg tctaaccagt cgccgctttc aattagcttt aatccctcga
      241 cgggtcgttg ataaacgtac atccatgcac tcccgtacgg cgtctgaatc aactggcgcg
      301 cgtattcacc gcccctggtg cgcaaggcat caagttcggc cagcgtggcg ttgtcaatac
      361 gataaacttc accgtgtacc gttccgttcc ccggaactgc gcctggatag tggcccaggc
      421 tatacaactg gtagttatcg atactgaaat cgcccagtaa ctgggcattg gtcatccagt
      481 gactgttgcc ttgtttgtgg cgtaaactgc cgtagacaaa tattcgcatt gctaaaactc
      541 gaactgatag agcaaatcca gtgcctggtc tacaccagac acggcttcca gatatagctt
      601 aggcatcagg cgataacgta acgtgagtgt tgctatagag tcaaatatac ccacgccgta
      661 tttcacttgc aggcctggca atacatagcc gctgaccact acctgggagg agtcgcctac
      721 tccctgggtg tcgagcgcta aattgcttac gccaaacgtc tcgccgattt tacccacaat
      781 ctggccactt tgcgcaaccc ccagaccaat cagcatcgag gtcattgccg cactgtcgct
      841 ctgatcgctc tccagccctt gtccacgtag caaataagac aatgcagctt gttgcgacat
      901 cgccgggtca gagaagatct ccgctttcgg ttcgtccgcc agaccagtga cgcgaactcc
      961 ggcgattacg tcgtcttctg tagcatccgg gttacgaata gcttcaatat taagatacgg
     1021 ttgatctggc ggaccagaga acagtagctc acccttacgc acaatcagat cctgaccata
     1081 ggcatggaag cgcccttcag ggatgttgat ctgcccgttc aggcccagcc cttgtttgtc
     1141 ctgaacgacg ttaagatcgc ccgtcagccg cgctttcagg ccaaaggcgt caatgcgcac
     1201 attgttgcca acgtggacaa tcaggttact gttaatcgga atcgacgccg ttttcggctc
     1261 ttccggttgc aggttatcgt taagcatcac cacatcgctg gagacgccta ctgcgctttc
     1321 cggcagatcg tgcaccacga tgcgcgccca cgggacatcc acgcgaccat cgagggtaaa
     1381 caggtttggt gtagcctcga atacaacatc tggcgataca tccattcgta ccatcggcgg
     1441 cacggtgatc cgcactttac tgcctttcgc cgttactcgt gcccgccagt tttcaatttg
     1501 gctccagtcg gcatcaccgt tcaggtagat ttcaccctgc tgggtccgta ctgtaccggc
     1561 aagcgtcgag cgcataccgt taaagttgac cgcaagctgg ctcggctgca tatcaaacgg
     1621 cataaagttg ccgtcgatat ccacacccgt aacctgaagc tgaccaaaca actgcgggct
     1681 ttgcacatca ccacccagac gcaagttggc actcaccatc cccgctgctt tttccccacg
     1741 agtaaagatg gggtttatca tcgcaaggtt gaagttacgg atattgacgt tgccaccaag
     1801 attacggcgg ccttgcggat cggtcaccag cacctgtcca tcaaactggc cgttattggt
     1861 caggcggatg gtccagccca attcggcacg gttgttacgc aattccgccg tcagattcag
     1921 tgtctgaaac gccaccggca gcgccgcatc gttgacggtt tgcgttacct gcacgttacg
     1981 ccccgaaagg gtgatactgc cctgcggcag cccctctttc gtggtgtccc aggcaacatc
     2041 cgctttaccc gtgaagatac cgctggcctg agtggtttct ggcataaacg gtttcagcat
     2101 ggcgaagtcg aagcggttga gattcaccac cgcacgccct tcggcccccg catcgatagt
     2161 ttgcggcacg cacagttccg cattcgggtt aagccagcag tgtggcccga tgctgatttt
     2221 ttgctcctta ttgcggtaat ccagcgcaat atcgcgggtc agcgaccacg ggccgaccag
     2281 cgtctggaag cgggtattgc taagagttcc cttccagcgt tcttctttgc gatcaaaact
     2341 tcctgccaga ttaagctgcc cggagacagg ctcgccctga atccgcaact gtagctcgtg
     2401 ctgcttttcg ctgcctttgg cattcagggt gacgaggttg atatttacat ccggctgcga
     2461 aatttgctca acgcgtacgt cgagtttccc ggcgatctga tcggtggatt taatgtcgcc
     2521 ttccacgcga acctgcgtca cggaaagttc ctgccagcgc aggccgcgcg cggtgatatc
     2581 tgccagtagt tgtggcgctt ccaccgtgcc gcgtactttc accagccctt tcgctgtacc
     2641 gccaagcccc ggcagcgcgt tatccagccc cggcgcgttg atggtggcat caagattgag
     2701 atcttttacc cccagctcgc ctttcacttc ggcactgttt ggccccagtt ccagatgaag
     2761 cccagggatc atccactgca tataactgtt gcctttcagc gtgccgtcaa cgttcacttt
     2821 gttctgtttg acgttaccgg tcagcttcag ttctggcacc tccatctgcc aggtgccgcc
     2881 gtacaggcta ccgcgggttt taatcaagcc attgagtttc gacggccact ccgggaactc
     2941 tttggcggtg ttaatgccat taagcgttag ctcaccgcgc caactaatgg cctgctgcca
     3001 gtcgagcaac gccttgagtt cagttttccc ttccagcgcc gcgacggtga gtttgtcgag
     3061 attgacctgc tgttcattac ctttggcatc aagggtaatg gtggctggcg ggatctcctg
     3121 tcccttcact gccgtacgca tagagagcgt gtaatcggtc attttgccgg taagtttcag
     3181 tttcagatca tccgcctgat actgcttctc accagtgaac ggccagtaaa gctgtttgct
     3241 gttcacttcc acgttgagcg gcaatccggc ttccgccagt cgcgtctggg cgcgtaaatc
     3301 catatccacc ggaccggaaa gattaacgcc aatctccagc tgttcgcgca gcgcgccgcc
     3361 cacttttagc ttcacttttt cacctttcaa cggctccacg ttcagtgtgc tgttgagggt
     3421 gatatccacc ggccagttgt ctgacagctg cgccgtgccg ctggcgttga cgatcccctg
     3481 gttggaatcg atatccaggg cgtccagttt agtattgccg tcaatgctgc tcactttcag
     3541 cagcatggtg cgcacggtga tgtccgtgtc gcccgtcacg cgtaactgct cgcctttaaa
     3601 ctcttcaatg ttcaggttaa gcggcaaatg caagtcggtc atttccggca ataccgggcg
     3661 agaaaagaga tctttcagcg tttcgccgag cggcttttca tccggctgcg gattttcaat
     3721 tttcggttcg accacttctt cctgcgccac ttccgccact ttcggcagag caatcagcag
     3781 gcctttcagc gacgtcggtt tcagggtcag ggttttctcc tgccagttca ggccggaggt
     3841 gaagtccatc accgacaccg tggtgtcatc aatcttgatg ttgacgttgt ccagcgccac
     3901 ccgtgtcagg gtgatgggat acggcgtgga gagatccagc ggaccgctat cttcctcttc
     3961 ttcaacctgt tcagaaggag gcattttttt actgtcgatg ttgacctgaa tgtctttcag
     4021 cgccaggtca ttaatacaaa cgctactgtt ccacaggcac tcaagaccga ccgccagatg
     4081 cagattaccc gcttttaccg ccacgcctgg ctgctcataa cgaacgtcag acaaggtgag
     4141 atcgcgccag ccgccggtga ccttgccaat atccagtcct ggcacccagc gatccgccgc
     4201 tttaaataca agatgcaggc cgctggtggt gcccaccaga aacgccaccg atcccagcaa
     4261 cagtaagata acgataacca cgccgaggct gatttttttc cataaactca taattctggc
     4321 cccagaccga tgtaaaactg taacccgtgt tcgtctttat ccgcgaccgg tacggcaaaa
     4381 tcgagtttga ttggcccgac cggcgattcc cagcgcacgc cgaccccggt accggtttta
     4441 aagtcgctgc ggcgaatatc gcttaccgct tcgccactat cgacaaacac cgcgccccac
     4501 cattttccgg tcacgttgta ctggtattcc agcgatccgg ttatcaactt cgaggcccct
     4561 ttcaggtcac cgttggcgta tttcggggcg atagatttgt atttgtagcc gcgaatactg
     4621 cgatcgcccc cggcgaaaaa acgcagatcc ggcggtactt tgtcgaaatc accggtttca
     4681 atccagccca gcgtgccgcg tgtaacgaaa cgatggcgat cgtacagtgt gcggatccag
     4741 acgttctgcg cctggaaaac ggagaaatcg acatctgagc cccaggccgt gttggagtag
     4801 tcgatagagt agcgttgcga gtcgccccag gttggcatca ggccaccaca agaacgcgtg
     4861 cggctaatca tcaccccagg ataaaacagc atcgtggtgt tggtaatttc accctgagta
     4921 aagtggtcga gactccagcg caggttaatg gcacgctgcc aaccgctgga gagatcccag
     4981 tagcgagaag ccaccagcgt agtggagtca gattcggtat cgttcaggtc agtgcgctta
     5041 aaaccgccct gcaccaaata atattgttcc agtggattct tcagcagcgg cattttatag
     5101 ctgaagtcga gggtctgttc cggcgcggaa atactggtac tggtggtcaa actgtgaccg
     5161 tatgagttca tccacggctt tttccacgtc gctttcacgc gcggtcccac gtccgtagag
     5221 taaccgaccc cggtttcgat ggtgttttct gttcgcggcg aaaccacgcc cgtcaatggt
     5281 aatactttcg tttcgcgcgc tttatcaaat tgtggagcca ccaccaccga gttaaaccag
     5341 ccggtagcag aaagtcggcg gttcagttcc gccagatctt tcgattcata ctcatcgccc
     5401 tctttaaacg gcaccagatt ttgcaggtat tcatcgcgga tttgtgatcc ttcaaaggtc
     5461 acatgcccaa agcggtaacg ttcgccactg ttataatcaa tatcccagaa ggctttatgc
     5521 aggccgagcg caatgcccag ctgcgctttg gtaaattcgc tatcgaaata acctttacgc
     5581 aacgcaatac tggttaagga ctttttgaaa ttttcataat cgccctggtt cagtaccgta
     5641 ccaatagccg ggcgagtatc gagcaatttc aaatagtctt tatcggtccg cgcgccgccg
     5701 cgcaatacca catcggtgcc gccaattaac accggcacac ctggcgtgac tttggcgatc
     5761 aatacctgcc gccctttctt tggcggtgga cggagatcaa attcaatggt cggctggtaa
     5821 taacccagcg ctttcagacc ttcacggatg gcatcatcga cgcgtgcgcg aaagcggcgg
     5881 tctggcgtca cttcatcact ttcaatcgta gaaagctgcg cacgaacgtt cttttccagc
     5941 tgtcccgata acccctcgac ctgtagacgg acgttcgcgg cgacggcaga tccgcttaag
     6001 cagagtaagc ttacacagca taactgtcgg atatagcgca cattttctcc tgaatatcct
     6061 tttttcctgc ccctggaacg ccgttaaacg gcttaacaaa aatccagtaa tatggattaa
     6121 aaagcagact aaaccccaaa tatttcttat gttttacttt agacctattc acggtggtta
     6181 ttgtgtgcaa atacgcctct tgttacaacc ttaaccccaa tgaccgattt tcgggagagc
     6241 gacaccatga gtttatttga taaaaagcat ctggtttccc ccgccgatgc cctgcctgga
     6301 cgtaacaccc cgatgcccgt agccacgctg catgcggtca acggtcactc aatgaccaat
     6361 gaacctgacg gaatggagat tgccattttt gcgatgggtt gtttctgggg tgtggagcgt
     6421 ctgttctggc agttacccgg cgtttacagc accgccgcag gctataccgg cggctatacg
     6481 ccaaatccga cttatcggga agtgtgctcc ggtgatacgg gtcatgccga agcggtacgc
     6541 attgtttacg atccttccgt catcagctat gagcagttgc tacaggtatt ttgggagaat
     6601 cacgatcccg cccagggaat gcgtcagggc aatgaccacg gcacgcagta tcgttcagcg
     6661 atttatccgc tgaccccaga acaggatgcc gcagctcgcg ccagtctgga acgttttcag
     6721 gcggcgatgc ttgccgccga tgacgatcgt cacatcacca cggaaatcgc taacgccaca
     6781 ccgttttatt atgccgaaga tgaccaccag caatatctgc ataaaaaccc atatggttac
     6841 tgtggaattg gcggaattgg cgtctgtctg ccgccggaag catagcgtta cgggtacaaa
     6901 tgtagtttgt tgataaagtg ctctttgtct atgcctgatg cggcttaaac gacttaccag
     6961 gcctacaaaa tcgtccaaat tcaatatatt gcaaggactg cgtaggcctg atcggcatag
     7021 cgcatcaggc agtattgcat ttatcagcag tctgaatttt aaccctctgg cgactttaca
     7081 gtaccttacg ctatactagc cactgaaaat tccggttcac tttcttcgaa tcggctttca
     7141 atgtgtattt cacacaaatt aatcaacttt cccttccgag gatctggcct gaaaggtcgg
     7201 ataagatatg ttaaacagta ttttagtcat actctgcttg atcgctgtaa gtgcgttctt
     7261 ctcgatgtcc gagatctcgc ttgccgcctc acgcaaaatc aaacttaaac tgctggctga
     7321 tgaaggcaat ataaatgccc aacgcgttct gaatatgcag gaaaatcccg gcatgttctt
     7381 taccgtggtc caaatcggtc tgaacgcagt ggcgattctc ggcggtatcg tcggtgatgc
     7441 ggcattttct ccagcttttc acagcctgtt ctcccgctat atgtcggcag agctctctga
     7501 gcaactgagc tttattctct ctttctcgtt agtaactggc atgtttatcc tgtttgcgga
     7561 tttaaccccg aaacgcatcg gtatgattgc gccagaagcg gtggctttgc gtatcatcaa
     7621 cccgatgcgc ttctgcctgt acgtttgcac cccgctggtg tggttcttca acggcctggc
     7681 gaacatgatc ttccgtattt tcaaactgcc aatggtacgt aaagatgaca tcacttctga
     7741 tgacatctac gcggtagtgg aagccggtgc gctggcgggc gtgttacgta aacaggaaca
     7801 cgagctgatt gaaaacgtct ttgagctgga atcccgtacc gttccgtctt caatgacacc
     7861 gcgtgaaaac gtgatttggt ttgatctcca cgaagatgag caaagcctga agaataaggt
     7921 ggcggaacat ccgcactcta agttcctcgt ctgtaatgaa gatattgacc acatcatcgg
     7981 ttatgtcgat tctaaagacc tgctgaaccg cgtgctggct aaccaaagcc tggcactgaa
     8041 cagcggcgta caaattcgca acacgctgat tgtgccggat acgttaaccc tttcagaggc
     8101 gttggaaagt tttaaaaccg caggtgaaga cttcgcggtg atcatgaacg agtacgcgct
     8161 ggtggtgggg atcatcaccc tcaacgacgt gatgaccacg ctgatgggcg atctggtcgg
     8221 tcaggggctg gaagagcaga ttgtcgcccg tgatgagaac tcatggctga ttgacggcgg
     8281 caccccaatt gacgacgtca tgcgcgtgct ggatattgac gagttcccgc agtcgggcaa
     8341 ctacgaaacc atcggcggct ttatgatgtt tatgctgcgt aagatcccga aacgcaccga
     8401 ttcggtgaaa ttcgccggct acaaatttga agtggtggat atcgataact accgcatcga
     8461 ccagctgctg gtgacccgga tcgacagcaa ggccaccgcc ctttcgccaa aactgcctga
     8521 cgctaaagat aaagaagaaa gcgtcgcgta acccccagaa acatcaacgg ctcctgaatc
     8581 aggagccgtc ttattactgc atagcacttt ggttaagcca tctctgtttg cagacgcata
     8641 acctgacggt taacttcgga catcactgac aaatgcagct tatccttcac ttttgggata
     8701 agaatcttac ccttatcaaa ttcaaaagcg ccaacgtcct tgatgtataa ccgtccacgg
     8761 aacaggatct ttacgtactt cgccacctga agtgggttgt accgttggaa aattttcatt
     8821 gttatctcct gctgagtatt acgcctgtgc ggtgccacaa tcggcccaac tattatgaga
     8881 cgcaaatttt aatgcttagt gactatagac tatccgggca atgtttccac cgcgtataac
     8941 tttttttacc taaaggttac aattattcag aattatcttt ttaccgaagc gcggtcttca
     9001 gtataagcat tcatttttca tatgatttgt gcgcttgacc gcaaactggc atcacacttg
     9061 cgggaaattc gataaatagc acatatgatt aaaactcaga tccaagtggt cggatcacct
     9121 gcatatcata agaaggaaac accatgaccc tacgcaagat tctggcactc acctgcctgc
     9181 tgttgccgat gatggcttcc gcacatcagt tcgaaaccgg tcagcgagtg ccgccgattg
     9241 gcattaccga tcggggcgag ttggtgcttg ataaagatca gtttagctac aaaacctgga
     9301 acagcgcgca gttagtggga aaagtgcgag tactgcaaca tattgctggt cgcacctctg
     9361 caaaagagaa aaacgcgacg ctgattgaag cgattaaatc agcaaagtta ccgcacgatc
     9421 gttaccagac caccaccatt gttaacaccg acgacgcaat tccgggttcc ggcatgtttg
     9481 tgcgcagcag tctggagagt aataaaaagc tttatccctg gtcgcagttt attgtcgata
     9541 gcaatggcgt cgcgcgcggt gcctggcagc tggatgaaga aagttccgct gtagtggtgc
     9601 tggataagga tggtcgcgtg caatgggcca aagacggggc gctcacacag gaagaggtgc
     9661 agcaagtgat ggacctgctg caaaagttgc tcaaataatg gcaataacat aaattctgca
     9721 aaaataattt tcccctatcg ttatctatgg gataggggaa ttaggtctct gagctcttac
     9781 accgaactca tactggtaac gctagttgct cttttttaaa cgcctcaatg gttgatttat
     9841 atttctcgca aatctcctct tcggtaatat cactatctac ggccagattc gcacttacta
     9901 tcgcatccac ttgttcggct aattcgactt gttggcaacc tgcattaacc agaaaattat
     9961 aaataagaga gtaatcttca tcctccaaaa caacagagga accattacga aaacgttcat
    10021 cccctccata gattttaagc gcaaagccca aagattctat gactatggca aaattatcat
    10081 caattatttt ttcgtaaata taccatccat cgcattcttt aattccacct cctggtattt
    10141 ggagttcaaa cttaccccgt tttaatttgc ttactatatc ctccaataag gcataaccta
    10201 aagacacgtc accaatcgcc tggtgaatat aatctttaat ccttttcggt atgtcagact
    10261 gttttttctg cagaatatcc caagcataca atcttctgtt ttgttctaac ggaaattcac
    10321 ggtttttgaa atccatgaat gagttatact catttattgt tatggggatt tcttcgtatt
    10381 caaatccact gcctcgcaat gtcattgtat attcggcacc gttatcttta aggctgaaac
    10441 tgataagaaa cttatccatg gctttaacag tttcattttt tgatgaaagc acttgataac
    10501 tatctttatc aatctgagtt gcgcaacgaa aaaactccca caaagttaac aggctatcat
    10561 aattcgaaac ccaatctttt ttcttaaatg catgctgaat attattaaaa aaattgtttg
    10621 aatgtgtttt tgtatgcgat gattttacga ataacaccac cgcaccaata aaattattgt
    10681 gtctgatcct gggtaacata tggctacctt attttatttg gttattttat gtcgccatta
    10741 aatatctcat tatagaagta aactatcaaa aaacgctcat tattaaaatg taacttatta
    10801 gcaattgaat ttttgcactt taaatacaat ctctttttat ttataacttt ttaggttggg
    10861 agaaatcagg atggcagaat caggaaatac actgtttctg ccatctgaat ttagtaaata
    10921 gacactctga acccaggatt cagaaacgac tcgcgcggag tgtaatccag cggtttaccc
    10981 tgccagtcgt gaacgtgcgc tccggcagct gcggccacag catgtccagc ggcggtgtcc
    11041 caaatattcg ttggtccgaa gcgcgggtac agctgcgcct gtccttccgc caccaggcag
    11101 aatttcagcg aagagccgat ggacgtggtc tgatgttcgc caagctgttg cagatactct
    11161 ttcagctccg catctgcatg ggaacggctg ataaccacca gcggcgggcg cgcatcgcgg
    11221 acctgaatct gcttgcgcac accgcactct tctttccagg ccttgccttc tgccgcgctg
    11281 tacattacgt tcattaccgg cgcatacacc acacctaaaa tcggtttgcc atggtcaatg
    11341 agcgcaatgt taacggtgaa ttcgccattg cgtttaataa actctttagt accatccagc
    11401 gggtctacca gccagtaacg ctgccagtgc tgacggactt cccaaccggg aggatcttct
    11461 tcagaaagga ccggaatatc cggtgtcagc gtacgtaaac cgtccatgat aacggtgtgt
    11521 gcggcaatat ctgctgccgt taccggagaa ttgtccgctt tgctgacgac gtccatcggt
    11581 ttcgtcccgt cgtagacctg cataatggca tcgcctgcat tccgtgcaag ctggcatact
    11641 tgatctaaca tttctccacc tcgtctctgt gagcggtgtt aacttattgt tttacttata
    11701 ccctatcgtt aatgaatgcg ccaactgtga tagtgtcatc attttcaaag cgtaaaattg
    11761 tggcattctt cactgttcta taagtaagac gtttattctc ccttttcttt cgtattcccg
    11821 atgataaaag gatgtccctg atgattaagt ttagcgcaac gctcctggcc acgctgattg
    11881 ccgccagtgt gaatgcagcg acggtcgatc tgcgtatcat ggaaaccact gatctgcata
    11941 gcaacatgat ggatttcgat tattacaaag acaccgccac ggaaaaattc ggactggtac
    12001 gtacggcaag cctgattaac gatgcccgca atgaagtgaa aaacagcgta ctggtcgata
    12061 acggcgattt gattcagggg agtccgctgg ccgattacat gtcggcgaaa ggattaaaag
    12121 caggtgatgt tcatccggtt tataaggcgc tgaatacgct ggattatacg gtcggtacac
    12181 tcggcaatca tgaatttaac tacggtctgg attacctgaa aaatgcgctg gcgggagcga
    12241 aattccctta tgtaaatgcc aacgtcattg acgccagaac caaacagcca atgtttacac
    12301 cgtatttaat taaagatacc gaagtggtcg ataaagacgg aaaaaaacag acgctgaaga
    12361 ttggctatat tggcgtcgtg ccgccgcaaa tcatgggctg ggataaagct aatttatccg
    12421 gaaaagtgac ggtgaatgat attaccgaaa ccgtgcgcaa atacgtgcct gaaatgcgcg
    12481 agaaaggtgc cgatgtcgtt gtcgttctgg cgcattccgg gctgtctgcc gatccgtata
    12541 aagtaatggc ggaaaactca gtttattacc tcagtgaaat tccgggcgtt aacgccatta
    12601 tgtttggcca tgctcacgcc gttttcccgg gtaaagattt tgctgatatc gaagaggctg
    12661 atatcaccaa aggcacgctg aatggtgttc cggcggtaat gccgggcatg tggggcgatc
    12721 atcttggggt ggtcgactta caactcagta atgacagcgg taaatggcag gtgacgcagg
    12781 cgaaagcgga agcacggccg atttacgaca tcgccaataa aaaatccctc gcggcggaag
    12841 acagcaagct ggtagaaaca ctcaaagccg atcacgatgc cacacgccag ttcgtcagca
    12901 agccaatcgg taaatctgcc gacaatatgt atagctatct ggcgctggtg caggacgatc
    12961 cgaccgtgca agtcgtgaac aacgcgcaaa aagcgtatgt cgaacattac attcagggcg
    13021 atccggatct ggcaaaactg ccggtgcttt cagctgccgc accgtttaaa gttggtggtc
    13081 gcaaaaatga cccggcaagc tatgtggagg tggaaaaagg ccagttgacc ttccgtaatg
    13141 ccgccgatct ttatctctac cccaatacgc tgattgtagt gaaagccagc ggtaaagagg
    13201 tgaaagagtg gctggagtgc tccgccggac agtttaacca gattgatcct aacagcacga
    13261 aaccacagtc actcatcaac tgggatggtt tccgcactta taactttgat gttattgatg
    13321 gtgtgaatta tcagattgat gttacccaac ccgcccgcta tgacggcgag tgccagatga
    13381 ttaatgccaa tgcagagcgg attaagaacc tgacctttaa tggcaagccg attgatccga
    13441 acgctatgtt cctcgttgcc accaataact atcgcgctta cggcggcaaa tttgccggta
    13501 cgggcgacag acatatcgct tttgcttcac cggatgagaa ccgctcggtg ctgacagcgt
    13561 ggattgctga tgagtcgaaa cgtgcgggag aaattcaccc ggcggcagat aacaactggc
    13621 gtttagcacc gatagctggc gataagaaac tggatatccg tttcgaaact tccccgtcag
    13681 ataaagccgc agcgtttatt aaagagaaag ggcaatatcc gatgaataaa gtcgcgaccg
    13741 atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa gtaaaacact tctttttcgg
    13801 tctataaatt atcaaccgca tccggcattt attggcggat gcgatgctgg cgcatcttat
    13861 ccgccctacc agccatgcac cgtagaccag ataagctcag cgcatccggc agttatgccg
    13921 cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag tccgccagtg
    13981 cagcaacctt ttcgctcacc tgctcgccca gcggcgtgat gctatattcc acatgcggtg
    14041 gcaccaccgg atacgcgata cggttaagaa aaccatcctg ttctaacgcc tgtaacgact
    14101 gcgcaagcat cttttcgctc accccgccga ttttgcgccg caggtcgcta aagcgatgag
    14161 taccttcgcg tagcgccacc agaatcaaca ccccccaacg gctggtgacg tgtttcaaca
    14221 cctcgcgcga cgggcactgt tccgcaaaga gattaccctc tttcagttgt tgcgacaggc
    14281 taacctgact catttcatac ttaccttttt gtacgtactt actaaaagta agtttaggtg
    14341 ttagcgtatt taaacacaag acaaaacgat ggagacttcc catgatcgct attactggtg
    14401 ccactggcca acttggtcac tatattattg aatccttgat gaaaacggtt cctgccagcc
    14461 aaatagtggc tatcgttcgt aatccggcaa aagcccaggc tctggcagca caaggcatta
    14521 ccgtgcgtca ggctgactac ggcgatgaag ccgcactgac atctgcactt cagggagtgg
    14581 aaaaactact gctgatctct tccagcgaag tgggtcaacg tgccccgcag catcgtaatg
    14641 ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc ttataccagc ctgctacatg
    14701 cagatacctc cccgctcggc ctcgccgatg agcacattga gacggagaaa atgttggctg
    14761 attctggcat cgtttacacc ctgctgcgca acggctggta cagcgaaaac tacctcgcca
    14821 gcgccccggc agcactggaa cacggcgtat ttatcggtgc ggcgggcgat ggcaaaatcg
    14881 cctcagcaac gcgggcagat tatgcggcag ctgcggcacg cgtgattagc gaagccggtc
    14941 acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca cagttagcgg
    15001 cagagctgac caaacagagc ggcaaacagg ttacctatca aaatctgagc gaagccgatt
    15061 tcgccgcggc actgaaaagc gtcggactgc ccgacggact ggcggatatg ctggcggatt
    15121 ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag caaaacgctt agcaaattga
    15181 ttggccgccc aacgacaacg ttagccgaaa gcgtaagcca tctttttaat gttaataact
    15241 agttaattaa agtggcatcc tcccgcatcc tctctaataa tgacgggatg ccgggagcaa
    15301 tcatgtctgc ttcctgaact ttcttctgac agaccaatgg atgccagtaa tgattagcgg
    15361 cgtgctgtac gccctgttag cagggttgat gtgggggctt atttttgtcg ggccgttgat
    15421 cgtaccggaa tacccggcga tgttgcagtc gatggggcgt tatctggcgt tagggttaat
    15481 tgcgctgccc attgcctggc tgggacgcgt gcgtctgcgt cagttggcgc gtcgcgactg
    15541 gcttaccgcc ttgatgctca ctatgatggg caacctcatc tattacttct gccttgccag
    15601 tgccattcaa cgtactggtg cgcctgtttc cacgatgatt atcggcaccc tgccggtggt
    15661 gattcccgtt tttgccaatc tgctttatag ccagcgcgac ggcaaactcg cgtggggaaa
    15721 actcgccccg gcactggttt gtattggcat cggcctggcg tgtgtgaata ttgctgagtt
    15781 aaaccacgga ctccccgatt ttgactgggc acgttatacc tcaggcatcg tgctagcgtt
    15841 agtttccgtg gtctgctggg catggtatgc cctgcgcaac gcccgctggc tgcgggagaa
    15901 tcccgacaaa catccgatga tgtgggcgac ggcgcaggcg ctggtcacac tgccggtttc
    15961 tctcatcggc tatctcgtcg cctgttactg gctgaatacg caaacgccgg acttctccct
    16021 accttttggc ccccgtccgc tggtgtttat tagtctgatg gttgcgatag ccgtgctttg
    16081 ctcatgggtt ggcgcactct gctggaacgt cgccagccag cgattaccga cagtgattct
    16141 cgggccgctg atcgttttcg aaacactggc aggtttgctg tacacctttt tgatacgcca
    16201 gcaaatgccg ccgctgatga cgctgagcgg tatcgcgctg ttagtggttg gcgtggtcat
    16261 tgtagtcaga gcaaaaccgg aaaagccttt aactgaatct gtctcagaaa gttgacatac
    16321 tggctgtgag ttaaataagc ctctgctacg taagggttat agcttttgcc ttaaagatgt
    16381 atttaaaata catcttatct tattaagaat gaggtatcag ctatggctta tcgcgaccaa
    16441 cctttaggtg aactggcgct ctctattcct cgcgcttcag ctctgtttcg taaatataat
    16501 atggattact gctgtggcgg taagcagacg ctggcgcgcg cggcggcacg taaagaactg
    16561 gatgttgagg tcattgaagc tgaactggca aagcttgctg aacaaccgat tgagaaagac
    16621 tggcgtagcg ccccgctggc agaaatcatt gaccatatca tcgtgcgcta ccacgatcgt
    16681 caccgcgagc aactgccgga gctgatcctg caagcgacta aagtcgagcg cgttcacgcc
    16741 gacaaaccga gcgtgccaaa agggctgaca aaatacctga ccatgctgca tgaagagctt
    16801 tccagccaca tgatgaaaga agagcagatc ctcttcccga tgatcaaaca aggcatgggc
    16861 agccaggcaa tggggccaat cagcgtaatg gaaagcgagc acgatgaagc gggcgaactg
    16921 ctggaagtga tcaaacacac taccaataac gtcacaccgc cgccagaagc ctgcaccacc
    16981 tggaaagcga tgtataacgg cattaatgaa ctgattgatg acctgatgga tcacatcagt
    17041 ctggaaaaca atgtactgtt cccacgcgcg ctggcgggtg agtgatctaa aagctggatg
    17101 gcattgcgcc atccagcatg ataatgcggt tatttccgca gttcagcagc ccgcttctta
    17161 ccaataaaca accagcccag ccccagtgcg ataaaccaca gcggggtaac cagcagcgcc
    17221 tggcgagtgt catcttccag tgtcagcaac acgaccacga acacaaagaa cgccatacat
    17281 acccagcaca tcagcttgcc gagcggcatc ttgtagatcg acttctcatg cagatgagga
    17341 cgctgtttgc ggtacaccag gtacgagcaa aggataatcg tccagacgaa cataaacaga
    17401 atcgcggaaa cggttgtaat catcgtgaac gcaccaatca cgctaggatt cacatacagc
    17461 atcaccacgc caccgagcag gcagatgcag gagaaggtca gccctttcgc tggtactgcg
    17521 cgcttagaaa gtttagcgaa cgctttcggt gccacacctt cctgcgccag accaaacagc
    17581 atacggctgg tagagaagac gccgctgtta gcggaagacg ccgcagaggt cagcaccaca
    17641 aagttgatca cgctggcagc cgcaggcaaa cctaccaaca caaacagctc aacgaacggg
    17701 cttttctccg ggactaccga actccacggt gtcacggaca taatcacaat cagcgcgaag
    17761 acgtagaaca taatgatacg gatcggaatg gagttaatcg cgcgtggcag tgatttctcc
    17821 ggatctttgg tttccgcagc tgttgtacct accagctcaa tccccacgaa agcgaaaacc
    17881 gctatctgga atccggcaaa gaagccactt aaacctttcg ggaaccagcc gccgtcattc
    17941 cacaaatgcg cgaatgacgc ttccacacca gtcggtgact gaaagtgcat cgccaccatg
    18001 accaggccga cgacaatcag cgacacgatg gcgacgattt tgatcatcgc aaaccagaac
    18061 tccatctcac cgaacatttt cacggtggca aggttgagcg tcagcagcag cactatcacc
    18121 gccagcgagg cgacccagtc ggagagatcg gggaaccaga actgagcata agccgtgatc
    18181 gccaccacgt ctgccatacc ggttacaacc cagcagaacc agtaagtcca gccagtgaaa
    18241 tatcctgccc acggcccgag taaatcggaa gcgaagtcac taaaagattt gtattccaga
    18301 ttcgaaagca gcaattcccc cattgcccgc atcacgaaaa agagcataaa accaatgatc
    18361 atataaacga aaatgatcga cggcccggca aggctaatcg ttttgccaga ccccagaaac
    18421 aaccccgtac caatggcacc gccaatggca ataagctgaa tatgtcggtt tgtgagattg
    18481 cgccgtagcg actgttcagc cggagcctga tcatcggcaa cgacttttac ctgatctacc
    18541 atgttttttt cttcctgtac ctgtctgtgt tgttcaggct ctacggcctt tagtcagtct
    18601 atgatacgac gaatctgtat cgggactcat cgatattagg taacaatacg cgggatgaat
    18661 actaagattt agatctaatg ttaattttat gtttaaagtg agtgtcatat cacggtgata
    18721 acgcgaaaca tctcacaaaa atacacgctt acggggtctt tgcaacacaa aatagcgccg
    18781 tttggcaaac tgaagggttt attgctgaat gcctgctccc ctctggttcg aggggaacag
    18841 aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa caccagggtg ctgaacggag
    18901 ggatggatgc acctgcgccg cgctcgccat atgccagttc ctgcgggata gtcagttccc
    18961 atttagaacc taccggcatc agagtcagtg cttcaatcca gccagggatc acgccgttaa
    19021 ccgggaattc agcgggttca ccacgagcaa cggagctgtc aaacacggtg ccgtcgatca
    19081 gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc cggaattgcg ccttcaccct
    19141 ggttgatcac gcggaattgc aggccagatt cggtgctatt cacaccttct tttttggcgt
    19201 tttcttccag gtatttcaca ccttcagcag ccatcgcctg gaaacgctga cgacgaacgg
    19261 catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac cacatcaacc ggaacagccg
    19321 gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag tgcttctggc agcagccctt
    19381 ccaggccaga ttcactcagt tgttgcccta cctgcaaacc aatgccgtag cttgcttgcg
    19441 cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc tttcttaggg tataaaagta
    19501 gcgggcagca tatcagccac acttctttgg gtaaaacttt gtcacaggaa tgatgacaaa
    19561 tcccggggaa aaagaaacaa tagcaatatc ccg
//
LOCUS       DADLTD010000063        19411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLTD010000063 DADLTD010000000
VERSION     DADLTD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.063"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..183)
                     /locus_tag="JN701_001779"
     CDS             complement(<1..183)
                     /locus_tag="JN701_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364251.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSV"
     gene            complement(219..1073)
                     /locus_tag="JN701_001780"
     CDS             complement(219..1073)
                     /locus_tag="JN701_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HAY7364252.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1417..2247
                     /locus_tag="JN701_001781"
     CDS             1417..2247
                     /locus_tag="JN701_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7364253.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2279..3214
                     /locus_tag="JN701_001782"
     CDS             2279..3214
                     /locus_tag="JN701_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7364254.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            3307..3549
                     /locus_tag="JN701_001783"
     CDS             3307..3549
                     /locus_tag="JN701_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY7364255.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3539..4990
                     /gene="panF"
                     /locus_tag="JN701_001784"
     CDS             3539..4990
                     /gene="panF"
                     /locus_tag="JN701_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY7364256.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            5002..5883
                     /gene="prmA"
                     /locus_tag="JN701_001785"
     CDS             5002..5883
                     /gene="prmA"
                     /locus_tag="JN701_001785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY7364257.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            6212..7177
                     /gene="dusB"
                     /locus_tag="JN701_001786"
     CDS             6212..7177
                     /gene="dusB"
                     /locus_tag="JN701_001786"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY7364258.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            7203..7499
                     /gene="fis"
                     /locus_tag="JN701_001787"
     CDS             7203..7499
                     /gene="fis"
                     /locus_tag="JN701_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY7364259.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7585..8469
                     /gene="yhdJ"
                     /locus_tag="JN701_001788"
     CDS             7585..8469
                     /gene="yhdJ"
                     /locus_tag="JN701_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7364260.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            8553..8732
                     /locus_tag="JN701_001789"
     CDS             8553..8732
                     /locus_tag="JN701_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY7364261.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8735..9397)
                     /gene="acrS"
                     /locus_tag="JN701_001790"
     CDS             complement(8735..9397)
                     /gene="acrS"
                     /locus_tag="JN701_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HAY7364262.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            9796..10953
                     /gene="acrE"
                     /locus_tag="JN701_001791"
     CDS             9796..10953
                     /gene="acrE"
                     /locus_tag="JN701_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HAY7364263.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10965..14069
                     /gene="acrF"
                     /locus_tag="JN701_001792"
     CDS             10965..14069
                     /gene="acrF"
                     /locus_tag="JN701_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HAY7364264.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSMKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            14322..14543
                     /locus_tag="JN701_001793"
     CDS             14322..14543
                     /locus_tag="JN701_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364265.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            14974..16035
                     /locus_tag="JN701_001794"
     CDS             14974..16035
                     /locus_tag="JN701_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7364266.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERF
                     TALQSGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGAT
                     VCIQAGTDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALR
                     IKLSNPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDE
                     KAANPATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIK
                     RGQNNLWNNGGIQYAPPVR"
     gene            16103..17273
                     /locus_tag="JN701_001795"
                     /pseudo
     CDS             16103..17273
                     /locus_tag="JN701_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            17283..18386
                     /locus_tag="JN701_001796"
     CDS             17283..18386
                     /locus_tag="JN701_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7364267.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            18394..19152
                     /locus_tag="JN701_001797"
     CDS             18394..19152
                     /locus_tag="JN701_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY7364268.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
BASE COUNT         4762 a         4619 c         4863 g         5167 t
ORIGIN      
        1 gacacttaaa ttgacattgt ctttttttgc ctcttctttc atggcattcg caagatcaat
       61 atagaaagga tgttgctgtg ttaataatga cgcaccaatt ttatactctt ttgccaacga
      121 attttgcgag aacaatgcca ggctcaccaa catagctgaa ccaagtaatt taatattttt
      181 cataaacaat cctcattgtt tttgtagggt acaacgtctt aaatttttcc ttcactacca
      241 cagacaccaa taatacgagt tacgacttct ttcatggcgg cctttgcggg ctgcatatag
      301 tggcgtggat catttgccgc aggattgttg tagaaatatc cttttaacgc atcggcaaac
      361 gcaattttca attctgtcgc gacattcact ttacaaatac cgcggcgaat acagtgctga
      421 acgtcctgtt ctgataagcc cgaagcacca tgcaacacca gtggaatctc cactcgctga
      481 cgaatctgtt ccagccgcac aaaatccagt cgtggttcat gcgcatataa accatgtgct
      541 gtgccgattg ctacggccag ggagtcaatt tgggtacgtt gaacaaactc tgcagctgcg
      601 gctggtgagg tataagcgga atctttttca tccaccagca gatcgtcctc ctggccgcca
      661 agacgcccta gctcggcctc aacgctaaca tcaaagcgat ggcaaaaggt ggtaacttct
      721 tgcactcgcg caatgttgtc ttcaaatggc aaatgggagc catcaatcat gacagaacgg
      781 atccctgcgc gaaccttgtt aaaaatgtca tcactctctt catgatggtc gagatgcagg
      841 actaacggca ggttgtactc gcgagccagc gattgtacta acgaaattaa acttcccacg
      901 ccaccataac gataagtacc gggtgttccc gccagcataa cgggagagcg aagttgtgca
      961 gcgctctcaa caactacctg taaagtttca aggttatgaa tgttgaaagc ggggacggca
     1021 tatccctctg actgggcttt tttcagcatg tttttgcttg aaatgatgta catcgcagct
     1081 cctgttattt gtcgttcgcc ttacaaaatg aagaaagaga atattcactt tcattttgaa
     1141 agcaaactag cacagcaaaa gcggcaaaaa gcgtgataca tgtcgaatat catcaaaata
     1201 acttacaaaa aaaataactt aatttgtttt tcactttaca agtgaaacca ataacatctc
     1261 tgcataaata tgaataaatt catttatttt tcaatgaatt aatctatttt tgttgcgatt
     1321 tttgacccat gattttcttt tcgaattgtc gatattattg aaggataact ttcaaagaaa
     1381 aatacaaggg gctatagtgt tacgcattaa aggccaatga agagtgttaa cgtgccgaga
     1441 aaatcgtcta acctactgaa acgcagatta aaaattgcag aaattgttgc cgcccaaggt
     1501 gaaatcaaag ttgatgactt atctgcgcaa cttggtgtct ctggcgtgac gatccgcggc
     1561 gatctcagtt atctggagca acagggatat ttaaagcgat catttggcgg tgctattgcg
     1621 acaccactct cctctgaacc agaagcgata caaccgatcg cgccattgtc tctggcacaa
     1681 caaatggaaa tagcacgaca ttgcgcacgg ttaattcgcg atcgtgacac gctatttctt
     1741 ggacatggga caatctgccg taagatcatt ccattgctca gcgaagtgaa aaaactgcgt
     1801 ctgatcacca acgatcccga acatgcgctg ctggcaaatc agtttattga tggcgaaata
     1861 atactggctg gcagtgaaat gctgcgcccc gacaccgcac tggccggaaa accgcttgaa
     1921 caggcacttc agcatttcac cattacacat agcattctgg aaatcaacca tctggatgct
     1981 gatggtactt taaatatcaa cgttcccaga ctggccgctg cctggcagct ctgtttcgat
     2041 catgcccaga ataagacggt tatcgttgcc gccgatcctg cgccatcaac ggctgctatc
     2101 gggcatttgc atcaggcaga aaatgtgatc gtcagccgca gcattaacga acaatatcag
     2161 caacaattgc agtcggctga tttcgttatc ctttatacca gtaatgagtg ctttacctgg
     2221 tccaatcgcg aaaggcttca ggaatgagag gggttttgtg acaataacta tctgcgctat
     2281 gggcgaattg ctggccgagt ttttgtcccg caacccacat caaaaattca ctcagcctgg
     2341 ggagtttatc gggccatttc ccagcggtgc gccagcaatt tttgctgctc aggtggcaaa
     2401 actgtcccat cgggccatct tcttcggatg tgttggtaat gatgattttg gccgactcat
     2461 tatagagcgc ctccgtcatg aaggtgtcat taccgatggg atccatgtta tgaacaatgc
     2521 cgtcacaggt acggcgttcg tgagttatca aaatccccag cagcgcgatt tcgtctttaa
     2581 tatccctaac agcgcctgcg gcttgtttac tgctgagcac attgataagg atctgctaaa
     2641 acagtgtaac catctccata ttgtgggctc atcattgttc tcatttcgca tgatcgatgt
     2701 catgcgtaaa gcaataacga cgatcaaatc ggctggcggc accgtttctt tcgatcccaa
     2761 tattcgcaaa gagatgctga gcattcctga aatggcgcag gctctcgatt atttgattga
     2821 atatacggat attttcatcc ccagcgaaag cgaactccct ttcttcgcgc gtcacaaaaa
     2881 tctgtcagaa gaacagattg ttagcgatct tctccacggc ggcgtaaaac atgtggcgat
     2941 aaaacgcgcc cagcgtgggg ccagctatta caagcttaaa aacggtacat tacacgccca
     3001 gcatgttaca ggccacgata tcgaaattat cgatccaacg ggtgcaggcg actgctttgg
     3061 cgcaacgttt atcactcttt tcttatccgg tttcccggca cataaggcac tgcaatatgc
     3121 aaatgccagc ggcgcgctcg ccgtaatgcg gcaaggtccg atggaaggga tatcctcact
     3181 ggctgacatt gaagactttt tgcagcagca ctgaacggca attttctgtc agattatttt
     3241 ccctgcctga agagttgccg tagaattgcc cgtttctcgt atccccccat gaggaggaac
     3301 ccttccgtgg acactcgttt tgttcaggcc cataaagagg cgcgctgggc gctggggctg
     3361 acccttttgt atctggcagt ttggttagca gccgcttact tacctgacgt tgcccccggt
     3421 tttaccggct ttccgcgctg gtttgagatg gcctgcatcc tgacgccgct gctgtttatt
     3481 ggactgtgct gggcgatggt gaaatttatc tatcgcgata tcccactgga ggatgacgat
     3541 gcagcttgaa gtgattctac cgctggtcgc ctatctggtg gtggtgttcg gtatctctgt
     3601 ttatgcgatg cgtaaacgga gcaccggtac cttccttaat gagtatttcc tcggcagccg
     3661 ctctatgggc ggtattgtgc tggcgatgac gctcaccgcg acctatatca gtgcaagttc
     3721 gtttatcggc gggcctggag ctgcttataa atacggcctg ggttgggtat tgctggcgat
     3781 gattcagctt cctgcagtct ggctttcgct cggtattctc ggcaagaagt tcgcgattct
     3841 tgcgcgccgc tacaatgcag tgacgctgaa cgacatgctg tttgcccgct accagagtcg
     3901 tcttctggtg tggctggcga gtttgagttt gctggttgcg ttcgttgggg caatgaccgt
     3961 gcagtttatc ggcggtgcgc gcctgctgga aaccgcggcg ggtattcctt atgaaaccgg
     4021 gctgctgatt ttcggtatca gcattgcgtt gtataccgcc tttggcggct ttcgcgccag
     4081 cgtgctgaac gacaccatgc aagggcttgt gatgctgatt ggcaccgttg tgctgcttat
     4141 tggcgtggta catgccgcag gtggtttaag taatgcggta gagacgctgc aaaccatcga
     4201 tccgcaactg gttacgccac aaggcgctga cgatattctg tcgcctgcct ttatgacgtc
     4261 gttctgggta ctggtgtgct ttggcgtgat tggcctgccg catactgcgg tgcgctgtat
     4321 ctcttataaa gacagcaaag cggtacaccg ggggatcatc atcggtacga ttgtagtcgc
     4381 aatcctgatg ttcggtatgc acctggccgg agcgttaggt cgtgcagtga tccccgatct
     4441 caccgtaccg gatctggtga tcccgacgtt aatggtaaaa gtgctgccac cgtttgctgc
     4501 cgggatcttt ctggctgcac ctatggctgc gatcatgtcg acaattaacg cccaattgct
     4561 acaaagttcc gctacgatca ttaaagatct ctatctgaat atccgtccgg atcaaatgca
     4621 aaacgagacg cgtctgaagc ggatgtcggc ggtaattacg ttagttctcg gcgcgttgct
     4681 gctgcttgcc gcctggaagc cgccagaaat gatcatctgg ctgaatttgc tggccttcgg
     4741 tgggttggaa gccgttttcc tgtggccgct ggtgctgggt ctttactggg aacgcgccaa
     4801 cgccaaaggc gcgctaagtg cgatgatcgt tggcggcgtg ctgtatgccg tactcgcgac
     4861 gctgaatatt cagtacctgg gcttccaccc tatcgtgccc tcgttactac taagtttgct
     4921 ggctttcctg gtcggaaacc gtttcggtac atccgtcccg caagctaccg ttttgactac
     4981 tgataaataa agagttttgc catgccttgg atccaactga aactgaacac caccggcgcg
     5041 aacgctgaag atcttagcga tgcgctgatg gaagcgggtg ccgtttctat cacttttcag
     5101 gatacccacg atacgccagt atttgagccg ctgccgggcg aaacgcgcct gtggggcgac
     5161 accgatgtga ttggtctgtt cgacgctgaa accgatatga acgacgtggt ggcgattctg
     5221 gaaaaccatc cgctgctcgg cgcaggcttc gcgcataaaa tcgaacaact ggaagataaa
     5281 gactgggagc gcgaatggat ggataatttc cacccgatgc gctttggtga acgactgtgg
     5341 atctgcccaa gctggcgtga tgtgccggac gaaaacgccg tcaacgtgat gttagaccca
     5401 gggctggcgt ttggtacggg tacccatcca actacctctc tgtgcctaca atggctcgac
     5461 agcctcgatt taactggtaa aacagtcatc gactttggct gcggttccgg cattctggct
     5521 atcgcggcgc tgaaactggg tgcagcaaaa gccattggta ttgatatcga tccgcaggcg
     5581 attcaggcta gccgcgacaa cgccgaacgt aatggcgttt ctgaccgtct ggaactctac
     5641 ttaccgaaag atcagccaga agaaatgaaa gccgacgtgg tggtcgcgaa catccttgca
     5701 ggcccattac gtgaactggc accgttaatc agcgtcctgc cggtttcagg cggtttgctg
     5761 ggcctttccg gtattctggc aagccaggca gagagcgttt gtgaagctta tgccgatagc
     5821 ttcgcactgg acccggtcgt ggaaaaagaa gagtggtgcc gtattaccgg tcgtaagaat
     5881 taaccttcgc atcgccgtag ggtgacgcgg ggcgaagtgc gagcaagctc acaaaaggca
     5941 cgtaaatttg ccggttattt acgcaaattt gcgtgccaaa attttcattc ataaagaaaa
     6001 attgagaact tactcaaatt tctttgaatg taaattttag tcactatttt ctaatatgat
     6061 gatttttatg agtaattatc gcaccacgct cattttaaat gcaattcttt gatccatctc
     6121 agaggattgg tcaaagtttg gcctttcatc tcgtgcaaaa aatgcgtaat atacgccgcc
     6181 ttgcagtcac agtatggtca tttcttaact catgcgcatc ggacaatatc agctcagaaa
     6241 tcgcctgatc gcagcgccca tggctggcat tacagacaga ccttttcgga cgttgtgcta
     6301 cgagatggga gccggattga cagtatccga gatgatgtct tctaacccac aggtttggga
     6361 aagcgacaaa tctcgtttac ggatggtgca cattgatgaa cccggtattc gcaccgtgca
     6421 aattgctggt agcgatccga aagaaatggc agatgcagca cgtattaacg tggaaagcgg
     6481 tgcccagatt attgatatca atatgggttg cccggctaaa aaagtgaatc gcaagctcgc
     6541 aggttcagcc ctcttgcagt acccggatgt cgttaaatcg atccttaccg aggtcgtcaa
     6601 tgcagtggac gttcctgtta ccctgaagat tcgcactggc tgggcaccgg aacaccgtaa
     6661 ctgcgaagag attgcccaac tggctgaaga ctgtggcatt caggctctga ccattcatgg
     6721 ccgtacacgc gcctgtttgt tcaatggaga agctgagtac gacagtattc gggcagttaa
     6781 gcagaaagtt tccattccgg ttatcgcgaa tggcgacatt actgacccgc ttaaagccag
     6841 agctgtgctc gactatacag gggcggatgc cctgatgata ggccgcgcag ctcagggaag
     6901 accctggatc tttcgggaaa tccagcatta tctggacact ggggagttgc tgcccccact
     6961 gcctttggca gaggttaagc gcttgctttg cgcgcacgtt cgggaactgc atgactttta
     7021 tggtccggca aaagggtacc gaattgcacg taaacacgtt tcctggtatc tccaggaaca
     7081 cgctccaaat gaccagtttc ggcgcacatt caacgccatt gaggatgcca gcgaacagct
     7141 ggaggcgttg gaggcatact tcgaaaattt tgcgtaaaca gaaataaaga gctgacagaa
     7201 ctatgttcga acaacgcgta aattctgacg tactgaccgt ttctaccgtt aactctcagg
     7261 atcaggtaac ccaaaaaccc ctgcgtgact cggttaaaca ggcactgaag aactattttg
     7321 ctcaactgaa tggtcaggat gtgaatgacc tctatgagct ggtactggct gaagtagaac
     7381 agcccctgtt ggacatggtg atgcaataca cccgtggtaa ccagacccgt gctgcgctga
     7441 tgatgggcat caaccgtggt acgctgcgta aaaaattgaa aaaatacggc atgaactaat
     7501 tcaggttagc taaatgcttg attaaaaagg cgctactcgg catggggaag cgcctttttt
     7561 ataggtgtca caaagggagt gaccatgaga acaggatgtg aaccgacccg gtttggtaat
     7621 gaagctaaga ccattattca cggtgatgcg cttgccgaac ttaaaaagat ccccgccgaa
     7681 agtgtcgatc tgatctttgc cgacccacca tataacatcg gtaaaaattt tgatggtctg
     7741 atcgaagcct ggaaagaaga tctgtttatc gactggctgt ttgaagtgat tgcagagtgc
     7801 caccgcgttc tgaaaaagca gggcagcatg tacatcatga acagcacgga aaacatgccc
     7861 tttatcgatc tccagtgccg caagcttttt accatcaaaa gtcgcatcgt ctggtcatat
     7921 gacagttctg gagtacaggc gaaaaaacac tacggctcca tgtacgaccc catcctgatg
     7981 atggtgaaag acgcaaagaa ctacacattc aacggtgatg ctattctggt agaagccaaa
     8041 accggatcgc agcgcgcgtt gatcgattat cgcaaaaatc ctccacagcc atacaatcat
     8101 caaaaagtac cgggtaacgt ctgggatttt ccgcgcgtgc gttatttgat ggatgaatat
     8161 gagaaccacc cgacgcaaaa accggaagcc ttactgaaac gcattattct cgcctcttcc
     8221 aacccaggcg atatcgttct cgacccgttt gccggtagct ttactaccgg tgccgtagcc
     8281 gtctccagcg ggcgaaaatt cattggtatt gagatcaaca gcgagtacat caaaatgggg
     8341 cttcgacggc tggatgtcgc gtcgcattac tctgcggaag aactggcgaa agtgaaaaaa
     8401 agaaagaccg gcaacctgtc aaaacgaagc cggttaagcg aagttgaccc cgatctcatt
     8461 gcaaagtaaa ggaagtgtaa gcctggtttt cagattattc atttcgtgta tatttcctgc
     8521 cagacttggt taaacatgca caggctcagg taatgattcg caagtattgg tggctcgtcg
     8581 ttttcgctgt cttcgttttt ctgtttgata ctttactgat gcagtggatt gaactgctga
     8641 caacagaaac agacaaatgc cgtaatatga actcagttaa tccactaaaa ctggttaact
     8701 gtgacgaact gaattttcag gacagaatgt gaatttacat gacacttaat tcattcgttt
     8761 gatgaattaa tttcgttatg ttttcatctg gcatgaacat tcttaatacg ttatcgacca
     8821 gcgcgggtgc ttgtttataa agatcataac ccgccatatt cattaaccag ttttgaacaa
     8881 ttccgctgaa ggcaccatta ataataatca tcacaacatc taaatcgagg ttatttgcta
     8941 cacaaccttg ttgctgacac gcctgcaata cttcgcggag agtctgcgga ttaaagccca
     9001 tcttttcgcg tatcactccc tcggccagca tctcatcatt aaattcacat ttgtgatata
     9061 agattttcag caacgcctgc tggcggggaa ttttggcaat atattgcaag ccgacaatca
     9121 atttttcacg caattgttga aacgggtcat gctctaatcc agccgtcaag tgttcctgga
     9181 ttaactcccg caatgaaggc tgttgcaacc acatctcatt aaacagttga gtcttgtttt
     9241 cgaagtgcca gtagatagcg ccacgcgtaa cgttagcggc gtcggcaatg tcgttgagcg
     9301 tcgtcttgct tacgccatgc tgcgcaaact gggcgatggc agtttcaatc agttcttgcc
     9361 gggtcttcag agcttcggct ttggttcttt ttgccatgat taattattca ggaaataaat
     9421 atattcgaca cagggtgaga aaatagcgaa ggttaatcta tcatctaatg tgtatttata
     9481 cgagagggta acattgagtt gctataaatc gtcaaataaa caatgtatat tatttaccta
     9541 aaatacattc gccacatgaa tatatatttc aatttacgag gttttaattc tgcctctttc
     9601 aacccgcgtc aaaataaaac agtagaatat taatcttttt ttgtgtttat gtgccttgag
     9661 acgccagtat tcataactat tccttacatc gacgaatgat aatttgtagg atagcaaact
     9721 gtatttttct ttctgcgagt taacgcgttg cctttctggg taaataacgc gcttttggtt
     9781 ttttgaggaa tagtaatgac gaaacatgcc aggtttttcc tcctgccctc ctttattctg
     9841 atctccgcgg ctttaatcgc cggttgtaac gataagggag aagagaaagc tcacgtcggt
     9901 gaaccgcagg ttaccgttca tattgtgaaa acggccccgt tagaagttaa gactgaatta
     9961 ccaggccgca ccaatgctta ccgtatagcc gaagttcgcc cacaggttag cgggatcgta
    10021 ctgaatcgta atttcactga aggcagcgat gtacaagcag gccagtccct gtaccagatc
    10081 gatcccgcga cctatcaggc aaattataac agcgcgaaag gcgaactggc gaaaagtgaa
    10141 gccgccgccg ccatcgcgca tttgacggta aaacgttacg ttccgctcgt gggtacgaaa
    10201 tacatcagcc agcaggagta cgaccaggcc attgctgatg ctcgccaggc cgatgccgcc
    10261 gtgattgccg caaaaggcac agtcgaaagc gctcgcatca atctcgctta taccaaagtg
    10321 actgcgccaa ttagcggacg tatcggcaaa tcgaccgtga ccgaaggcgc tcttgtcact
    10381 aatgggcaaa cgactgaact ggcgactgtc cagcagctcg atcctatcta cgttgatgtg
    10441 acccaatcca gcaacgattt tatgaggctg aagcaatccg tagagcaagg aaatttgcat
    10501 aaggaaaacg ccaccagcaa cgtagagttg gtcatggaaa acggtcaaac ctatcccctg
    10561 aaaggtacgc tgcaattctc tgatgtgacc gttgatgaaa gcaccggctc cataacccta
    10621 cgtgctgtct tccctaaccc gcaacatacg cttttgccgg gtatgtatgt gcgtgcacgg
    10681 attgatgaag gcgtccaacc tgacgccatt cttatcccgc aacaaggcgt tagccgcaca
    10741 ccgcgtggtg atgcaaccgt gctgattgtt aacgataaaa gtcaggtgga agcgcgccct
    10801 gtcgttgcca gtcaggcgat tggcgataaa tggttgatta gcgaaggtct gaaatctggc
    10861 gatcaagtca ttgtcagcgg cctgcaaaaa gcgcgtccgg gagagcaggt taaagccact
    10921 accgataccc ccgcagatac tgcatcgaag taaggtaatc tgacatggca aactttttta
    10981 ttcgacgacc gatatttgca tgggtgctgg ccattattct gatgatggcg ggcgcactgg
    11041 cgatcctaca attgcccgtc gctcagtatc caaccattgc accgcctgcg gtttctgttt
    11101 cggcaaacta tccaggtgct gatgcgcaga ccgtgcagga tacggtgacg caggttatcg
    11161 aacagaatat gaatggtatc gataacctga tgtatatgtc ctccaccaac gattccgccg
    11221 gtagcgtgac aattaccctt accttccagt ccgggaccga tcctgatatc gcgcaagtgc
    11281 aggtgcagaa caaactccag ctcgccacgc cgttgctgcc gcaggaggtt cagcagcagg
    11341 ggatcagtgt tgaaaagtcc agtagcagct atttgatggt ggcgggtttt gtctctgata
    11401 acccaggcac cacacaggac gatatctcgg actatgtggc ctctaacgtt aaagatacgc
    11461 ttagccgtct gaatggcgtc ggtgacgtac agcttttcgg cgcacagtat gcgatgcgta
    11521 tctggctgga cgccgatctg ctaaacaaat ataaactgac accggttgat gtgattaacc
    11581 agttgaaggt acagaacgat cagatcgctg ccggacagtt gggcggaacg ccagcgttac
    11641 cagggcaaca gttgaatgcc tcgattattg ctcaaacgcg gttaaaaaat ccggaagaat
    11701 tcggcaaagt gaccctgcgc gtaaacagtg acggctcggt ggtacgcctg aaagatgtcg
    11761 cacgggttga acttggcggt gaaaactata acgttatcgc tcgtatcaac ggaaaaccgg
    11821 cggcgggcct ggggattaag ctggcaaccg gcgcgaatgc tctcgatacc gcgaaagcca
    11881 ttaaggcaaa actggcggaa ttacagccat tcttcccgca gggaatgaag gttctctacc
    11941 cttatgacac cacgccattc gtccagcttt ctattcacga agtggtaaaa acgctgttcg
    12001 aagccattat gctggtgttc ctggtgatgt atctgttctt gcagaatatg cgagcaacgc
    12061 tgatccccac cattgcggta cccgtggtgt tgttagggac gtttgccatc ctcgccgctt
    12121 ttggttactc catcaacaca ctaacgatgt tcgggatggt gcttgccatc gggctgctcg
    12181 tcgatgatgc gatagtggtg gtggagaacg tcgagcgcgt gatgatggag gataagctcc
    12241 cgccaaaaga agcgacggaa aaatcgatgt cgcaaattca gggcgcactg gtgggtatcg
    12301 cgatggtgct gtcagcggta tttattccga tggcattctt cggcggttct actggggcaa
    12361 tttatcgcca gttctctatc accatcgttt cggcaatggc actttctgtt ctggtggcat
    12421 tgattcttac ccctgcgtta tgtgcaacgc tgcttaaacc cgtctctgct gagcatcacg
    12481 aaaataaggg cggtttcttc ggctggttta ataccacctt cgatcatagc gttaaccact
    12541 acaccaacag cgtcggcaaa atcctcggtt ccacaggacg atatttactg atctatgcgc
    12601 tgattgttgc aggaatggtg gtgttgtttt tacgtcttcc gtcttccttc ttacctgaag
    12661 aggatcaggg tgtctttctg accatgattc agttacccgc tggcgcgacg caagagcgga
    12721 cgcaaaaagt gttggatcaa gtgacggatt actatctgaa gaacgagaaa gcgaacgttg
    12781 aaagtgtctt tacggttaac ggctttagct tcagcggcca ggcacaaaac gccggtatgg
    12841 ccttcgtcag tatgaaaccg tgggaagagc gtaatggtga tgaaaacagt gcggaagcgg
    12901 taatccatcg tgccaaaatg gaattgggca agatccgcga cggttttgtc attccattca
    12961 atatgccagc cattgttgaa ctgggcacgg caacgggttt cgactttgag ttaattgatc
    13021 aggctgggct gggtcacgat gccctaaccc aggcccgtaa ccagttgctt ggtatggcgg
    13081 cgcaacatcc tgccagctta gtcagcgtgc gtcctaatgg cctggaagac accgcgcagt
    13141 ttaaactgga agttgaccag gaaaaggcgc aggcattaag tgtttcactt tctgacatca
    13201 atcagaccat ttcaacggcg ttgggtggga cttacgttaa cgacttcatc gaccgtggtc
    13261 gcgtgaaaaa ggtgtatgtt caggcggatg ccaaattccg tatgctgcca gaagatgtcg
    13321 ataaacttta tgtccgcagc gccaacggcg aaatggtgcc attctcggcc tttaccactt
    13381 cacattgggt gtatggctct ccgcgactgg aacgctacaa cggtctgccg tcaatggaga
    13441 ttcaggggga agccgcgcca ggaaccagtt ccggcgatgc catggcgttg atggaaaacc
    13501 ttgcgtcaaa attacctgcg ggcattggtt atgactggac gggtatgtcg tatcaggaac
    13561 gcttatcggg aaaccaggct cccgctctag tagcaatttc ctttgtggtt gttttcctgt
    13621 gccttgctgc actctatgaa agctggtcaa ttcctgtctc ggttatgttg gtcgtgccgt
    13681 tagggattgt cggcgtgctg ctggcggcga cactctttaa tcaaaaaaat gacgtctact
    13741 ttatggtggg cttgctaacg acaattggct tgtcggccaa aaacgctatt ttgattgttg
    13801 agttcgctaa agatctcatg gagaaagagg gtaaaggtgt tgttgaagcg acactaatgg
    13861 cagtacgtat gcgtctgcgt cctatcctga tgacctctct cgcctttatt ctcggcgtat
    13921 taccgctagc tatcagtaac ggtgcaggca gtggcgcgca gaacgcagtg ggtatcgggg
    13981 taatgggcgg aatggtctct gcaacgttgc tggcaatctt cttcctaccg gtgttctttg
    14041 tggtgatccg ccgttgcttt aaaggataaa tcagaaacat aaaggcactt tcgggtgcct
    14101 ttattatttt cagtgaaacc cataaaaatt aataagatat tcttccactc actctttaaa
    14161 aactttctat agttcccgct cccttcacta tttttacaat tcacataatt tgtcattatt
    14221 cattccgcaa tatttataac ctccggtgat aaaatggcat tcagctcgtt aataagagag
    14281 ttaacttatt aagcgttagc gtttattact gaggtaacac catgaaaaga ttaattcctg
    14341 tggcattgct caccgcattg ctggcgggct gtgctcacga ttcgccctgc gttccggttt
    14401 atgacgatca gggttgtctg gttcacacca atacctgtat gaaaggcacg actcaggata
    14461 actgggaaac ggctggggct attgccggcg gggcagcggc tgttgctggc ctgacgatgg
    14521 ggatcatcgc tctgtcgaaa taactgaaca gggcgcggag tttcgcgctc tgtttaacga
    14581 tacagtatct ttacataaga taaaatattg ctatattctg ctggctaatt ctctgctgat
    14641 ttagcatgtt atatcgcgtt atttcgctgc tttcgaaata acgcatcaat ttaactttca
    14701 cacttcccac cagaaatata ctcacgctaa caatcattat tctgcttagt aaaacacgcg
    14761 taaatatttt gcgccaaaat gtggcgcatg tttcattttc gcaccattgc ggggcgctgt
    14821 ttttattatt tcctgtctac actcagctta ttgcgacgcg aaatcgtgcg caaaagctgg
    14881 cactactttt gcttataaga ggatggccac agacaggtaa aagacgtttc ccaaacgtcc
    14941 tataacgata attttcgcct cacaggaagc attatgaaaa agatgatgat agccacactg
    15001 gctgccgcca gcgtgctgct tgccgttgca aatcaggcgc atgctggcgc gacgcttgat
    15061 gccgttcaga aaaaaggttt tgtgcaatgc gggatcagtg atggattact tgggttctct
    15121 tatgccgatg cagacggtaa gttttcaggt attgatgttg atgttgatgt tgatgttgat
    15181 gttgatgttg atgttgatgt ttgtcgtagt gttgccgctg ctgtatttgg tgacgacacg
    15241 aaagtgaaat ataccccact cacagcaaaa gaacgcttca cggctttaca gtcaggggaa
    15301 gtggatttgc tctcccgtaa tacgacctgg acttcatctc gcgacgccgg gatgggaatg
    15361 gcatttactg gcgtcactta ttacgacggc attggcttcc tgacgcacga taaagcgggg
    15421 ctaaaaagcg cgaaagaact ggatggcgct accgtctgta ttcaggcggg tactgatacc
    15481 gaactcaacg ttgccgacta tttcaaggca aacaatatga agtacacacc ggtgactttc
    15541 gatcgctctg acgaatcagc gaaggcactg gaatctggtc gctgcgatac gctggcctcg
    15601 gatcagtcgc aactgtatgc cctgcgcatc aaattaagca acccagctga atggattgtc
    15661 ttaccggagg ttatctctaa agaaccgctt gggccggtag ttcgtcgtgg cgatgatgaa
    15721 tggttctcga ttgtacgctg gacgcttttc gccatgctga atgctgaaga gatgggcatc
    15781 aattcccaga acgtcgatga aaaagcggct aatccagcaa cgcctgatat ggcacatctg
    15841 ctgggtaaag aaggcgatta cggcaaggat ctgaagttgg ataataaatg ggcctataac
    15901 atcatcaaac aggtgggtaa ctactcggaa attttcgagc gtaacgtagg ttcagaaagc
    15961 ccgctgaaaa ttaaacgtgg gcaaaataat ctctggaata acggcggtat tcagtacgcg
    16021 ccgcccgtgc gttaagtcgg tgtgaggtaa cgagcgtcgc tacggcggcg ctctcttcag
    16081 cgttatgctt tccgaggttt ctatgtctca tcgccgctca accgttaaag gctcactctc
    16141 ctttgccaac cctacggttc gtgcctggtt attccagatc cttgccgttg ttgctgttgt
    16201 cggaattgtt ggttggttat ttcacaacac tgtaacgaat ctcagtaatc gtggcattac
    16261 ttcaggtttt gcctttctgg atcgcggcgc tggcttcggt attgtccagc atttgatcga
    16321 ttaccagcag ggcgacacct acggacgcgt ttttattgtc ggcttactca atacgctact
    16381 ggtttctgca ttgtgtattg tgttcgcttc tgttttaggc ttctttatcg gtctgacgag
    16441 actttcggat aactgactgc tacgaaagct atccacaatt tatattgaga tcttccgtaa
    16501 tattcccccg ctgctgcaaa tcttcttctg gtactttgcc gtgttgcgca atttgcccgg
    16561 accacgcgcc aggctgtgag cgcgtttgat ctggcctttt tgagcaatcg tgggctttat
    16621 attccgtcac cgcagctggg agacggattt attgcgttta tcctggctgt tgttatggct
    16681 atagtccttt ctgttgggct attccgcttt aataaaacat accagataaa gaccggacaa
    16741 ctgcgccgca cctggccgtt cgccgcagtg ttgatcatcg gtttgccttt actggcgcaa
    16801 tggctttttg gcgcagcact acactgggat gtcccagccc tacgaggctt taacttccgc
    16861 ggcggaatgg ttttaattcc tgaactggca gccttaacgc tggcactttc ggtttatacc
    16921 tctgcattta tcgccgagat tatccgcgct gggatccagg cagtgcctta tggtcaacat
    16981 gaagcggctc ggtcactggg gttacgctac gccaggtcat tattccccag gcactgcgag
    17041 tgattattcc gccgttaacc agccagtatc tcaacatcgt caaaaactcc tctcttgccg
    17101 ccgctattgg ctatcccgat atggtttcgc tgtttgctgg caccgtgctg aaccagacgg
    17161 ggcaagccat cgagacgata gccatgacca tgtcggtcta tctgattatc agcctgacta
    17221 tctcgctgct gatgaatatc tataaccgcc gcatcgcgat cgttgaacgc taaggagtca
    17281 tgatgacaaa agtattgctg tctcagccct cgcgcccagc gagccataac tcaagccgcg
    17341 cgatggtgtg ggtgcgaaaa aatctgttct ccagctggag caatagcctg ctgactattg
    17401 gctgcatatg gttgatgtgg gaactgatcc caccgttgct gaactgggca tttttgcagg
    17461 ctaactgggt tggctcaacg cgtgccgact gcacaaaagc cggtgcctgt tgggtcttca
    17521 tccacgaacg atttggtcag tttatgtatg gactttaccc acacgaccaa cgctggcgaa
    17581 ttaacctggt attactgatt ggccttgtgt caatcgcacc aatgttctgg aaaatactcc
    17641 cgcatcgcgg tcgctatatt gcggtctggg cggtgattta cccactgatt gtctggtggc
    17701 tgatgtatgg cggttttttt ggtcttgagc gggttgaaac ccggcaatgg ggcgggctga
    17761 cgctaacttt aattattgca tccgttggga ttgcgggggc gctaccgtgg gggatcttac
    17821 tggcgttagg tcgccgctcc catatgccga ttgtgcgtat cttatcggtc atttttatcg
    17881 agttctggcg cggcgtaccg ctgattaccg tattgtttat gtcttcggtc atgctgccgt
    17941 tgtttatggc agaaggcacc agtatcgaca aactgatccg cgcgctggtt ggcgtgatcc
    18001 tgtttcagtc agcatatgtt gcggaagtcg tgcgaggcgg attacaggcc ctacctaaag
    18061 ggcaatatga agcggcagag tcgctggcgt tggggtactg gaaaactcag gggctggtta
    18121 ttctgccaca ggcgttgaag ctggtaattc ctgggctggt aaataccatc atcgcactct
    18181 tcaaagatac cagcctggtg atcatcatcg gcttgttcga tcttttcagt agcgttcagc
    18241 aggcaaccgt tgatcccgcc tggttgggta tgtcgacgga agggtatgtt ttcgccgcgc
    18301 tgatctactg gatcttttgt ttcagcatgt cacgttatag ccaacatctg gaaaaacgtt
    18361 ttaacaccgg gcgtacaccg cattgaggac actatgagcc aaattttact gcaacctgct
    18421 aacgcgatga ttacgctgga aaacgtccat aaatggtatg gacaattcca tgttttgaaa
    18481 aatataaatt taaccgtgca accgggagaa cggatcgttc tgtgtggccc ttctggttcc
    18541 ggtaaatcga caaccattcg ttgtattaat catctggaag aacatcaaca gggacggatt
    18601 gtcgtagatg gcatcgaact taatgaagat atccgcaata ttgagcgcgt caggcaagaa
    18661 gtgggaatgg tatttcagca tttcaatctc ttccctcatc tgaccgtttt acagaactgt
    18721 accctggcac cgatttgggt acgcaagatg cctaagaaag aggctgaagc tctggcgatg
    18781 cattaccttg agcgggtgag aattgccgaa catgcgcata agtttcccgg acagatttca
    18841 ggtggtcagc agcaacgcgt tgccattgcg cgttcgctgt gtatgaagcc gaaaattatg
    18901 ttgtttgatg aaccaacgtc ggcgctcgat ccggagatgg tgaaagaggt gctggatacg
    18961 atgattgggc tggcgcagtc gggtatgaca atgttgtgtg taacacatga gatggggttt
    19021 gcacgtaccg tcgctgaccg ggtaattttt atggatcgtg gggagatagt ggaacaagct
    19081 gcacctgatg aattttttac gcatcctaaa tcagagcgta cgagggcatt tttatcgcag
    19141 gtaatccatt aattgaatgt tagttcgaaa agcaaaaagg ccatcctttc ggatggcctt
    19201 tttgcttgat ttgatgtctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
    19261 gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcgag agagtgttca
    19321 ccgacaaaca acagataaaa caaaaggctc agtcggaaga ctgggccttt tgttttattt
    19381 gatgtctggc agttccctac tctcgcatgg g
//
LOCUS       DADLTD010000064        19332 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLTD010000064 DADLTD010000000
VERSION     DADLTD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.064"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(459..2108)
                     /gene="pgi"
                     /locus_tag="JN701_001798"
     CDS             complement(459..2108)
                     /gene="pgi"
                     /locus_tag="JN701_001798"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY7364269.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2633..3982
                     /gene="lysC"
                     /locus_tag="JN701_001799"
     CDS             2633..3982
                     /gene="lysC"
                     /locus_tag="JN701_001799"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY7364270.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4234..4506
                     /locus_tag="JN701_001800"
     CDS             4234..4506
                     /locus_tag="JN701_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY7364271.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4639..5511)
                     /gene="rluF"
                     /locus_tag="JN701_001801"
     CDS             complement(4639..5511)
                     /gene="rluF"
                     /locus_tag="JN701_001801"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY7364272.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5859..6806
                     /locus_tag="JN701_001802"
     CDS             5859..6806
                     /locus_tag="JN701_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7364273.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6876..7682
                     /locus_tag="JN701_001803"
     CDS             6876..7682
                     /locus_tag="JN701_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7364274.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7692..8099
                     /locus_tag="JN701_001804"
     CDS             7692..8099
                     /locus_tag="JN701_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY7364275.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            8099..8593
                     /locus_tag="JN701_001805"
     CDS             8099..8593
                     /locus_tag="JN701_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY7364276.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8659..9456
                     /locus_tag="JN701_001806"
     CDS             8659..9456
                     /locus_tag="JN701_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7364277.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVISLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9467..10309
                     /locus_tag="JN701_001807"
     CDS             9467..10309
                     /locus_tag="JN701_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY7364278.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            10343..11572
                     /locus_tag="JN701_001808"
     CDS             10343..11572
                     /locus_tag="JN701_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7364279.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11955..12644
                     /gene="pepE"
                     /locus_tag="JN701_001809"
     CDS             11955..12644
                     /gene="pepE"
                     /locus_tag="JN701_001809"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY7364280.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12735..14366)
                     /locus_tag="JN701_001810"
     CDS             complement(12735..14366)
                     /locus_tag="JN701_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY7364281.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(14586..18269)
                     /gene="metH"
                     /locus_tag="JN701_001811"
     CDS             complement(14586..18269)
                     /gene="metH"
                     /locus_tag="JN701_001811"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY7364282.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEAQDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTDNAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNCIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18469..19293
                     /gene="iclR"
                     /locus_tag="JN701_001812"
     CDS             18469..19293
                     /gene="iclR"
                     /locus_tag="JN701_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY7364283.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4590 a         5035 c         5054 g         4653 t
ORIGIN      
        1 aatttccctt cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta
       61 tacacaacta aagcatgcga attacggcgc ggggaattag cgatggcgtt ttaggataat
      121 tggaatgaat atcaaatata agcttcaact tatatttaaa taacaataaa aatcataaac
      181 ttaaaataaa aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa
      241 tctattttag gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat
      301 ctgataaaac aatgccgact tattgtcggc attgttttta aggttattta actcctcata
      361 ctatgcatta atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct
      421 tatccggcct acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag
      481 cggttaatca gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc
      541 tctggcagaa tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta
      601 aagatgttca ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgcc
      661 cccaggctga acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg
      721 aacactttga acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat
      781 tcctgctcaa ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag
      841 ttagacagca gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg
      901 aaatcgcacg gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg
      961 ttagtgcctg gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta
     1021 cggtcaacat acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg
     1081 tgcatatact ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac
     1141 cagatgccaa tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa
     1201 tgcttgtcca tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag
     1261 agaacaatcg acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag
     1321 aactcgaaca tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa
     1381 agcgccgcaa agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca
     1441 cgcgcgctat gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccaag
     1501 aacagcgtgg tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg
     1561 ttagaaacaa agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg
     1621 tacgggccga ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg
     1681 gtataaccct tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc
     1741 accgcgttga cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg
     1801 ttacgcagcg ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag
     1861 aacatcgact taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc
     1921 agcgtctctt cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt
     1981 gcggagaact tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct
     2041 ttcatttcat cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg
     2101 tttttcatta gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc
     2161 agtcacagaa aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta
     2221 gcgcagtgaa gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca
     2281 tgaccccgtc gttgacaacc gccctgctca ccctttattt ataaatgtac tacctgcgct
     2341 agcgcaggcc agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga
     2401 taacacctga gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc
     2461 tacaggggct gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag
     2521 tggtggagca cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc
     2581 cgctcttccc ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga
     2641 aattgttgtc tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag
     2701 cgctgatatt gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg
     2761 tatcactaat ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa
     2821 actcgacgct attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt
     2881 tatccgtgaa gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc
     2941 gctggcaacg tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac
     3001 cctgctgttt gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg
     3061 taaagtgatg cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc
     3121 ggaactggcc gcgctgcagc tgctcccacg cctcaatgac ggcttagtga tcacccaggg
     3181 atttatcggt agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta
     3241 tacggcagcc ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt
     3301 cccgggcatc tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat
     3361 cgcgtttgcc gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac
     3421 gttgctaccc gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg
     3481 cgcaggtggt acgctggtgt gcaataaaac tgaaaatcct ccgctgttcc gcgcgctggc
     3541 gcttcgtcgc aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg
     3601 cttcctcgcg gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac
     3661 cacgtcagaa gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga
     3721 tacgttgctg acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga
     3781 agaaggtctg gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg
     3841 caaagaggta ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc
     3901 cagccataac ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact
     3961 gcatagtaat ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt
     4021 gattttcttg tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt
     4081 atcgagaata attgccttta atttttttat ctgcatctct aattaattat cgaaagagat
     4141 aaatagttaa gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta
     4201 cactgaactc tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa
     4261 gagatgactg aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg
     4321 catggtcgcg tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa
     4381 ttaatggtcg agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct
     4441 tataaaccgg atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg
     4501 cgctaatgaa caatcaacgc ctgagatttt cactcgtctg tgtggacaca atcttcccct
     4561 caccctaacc ctctccccga aggggcgagg ggactgtccg agctcttttt ggacgttttc
     4621 atcagtctga ggcgttaatc agcgtccctt ctttttacgc cccggcgagg taaaacgctt
     4681 accgttggac gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg
     4741 tttgatgccc gctgttttcg gtttggcctt cgctttaggt tttgcctcgg aagaggaatt
     4801 ttcgatgagc ttaaagaggt cgattaactc atcgtcggtt aaatcgcgcc actcccccag
     4861 cggaatgccg cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata
     4921 gccgaaatgc tcgcacatgc gccggatctg acggttcagc ccctgcacca gggtaatgcg
     4981 gaagacaaac ggcgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg
     5041 cacccccgcc cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag
     5101 atactctttc tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt
     5161 ggtgaggaaa atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt
     5221 gctgtggtta acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat
     5281 accgacgggc ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa
     5341 ctgaccgttt actttcacaa tgtcgccggg tttcacctga tcgccaatgg tggctcgctt
     5401 gccattaagg aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat
     5461 tccgctttcg ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc
     5521 tgtaaaacgc ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt
     5581 cgaaattaaa tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc
     5641 aaatttgcct ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg
     5701 atgactacta cgcagagaaa caaatgtgca agagaaagcg ctatatctcc ccatgcaaag
     5761 agtgaaaaat cgctttgaaa ggccctcatg atggggttat atcagatcaa gcggtgagaa
     5821 agcgtatttg cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc
     5881 gtttgattgt gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg
     5941 cgcgcgaact gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc
     6001 agggaattgt caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc
     6061 aactgaagca aaagtttggc ctgaaagacg ttgtggtggt gtcgggaaat gatgaggatg
     6121 aagagactca actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg
     6181 agcctggcga tattgtcggt ttttcctggg gtcgcgcggt gagcgcactg gttgaaaact
     6241 tgccgcaggc ggggcaatcg cggcagttaa tctgcgtacc aattattggc ggaccgtccg
     6301 gtaaactcga aagccgctat catgtaaaca cattaaccta cagcgcggca gcgaagctga
     6361 aaggggaatc gcatctcgcg gattttccgg ctctgctgga taacccatta attcgtaatg
     6421 ggatcatgca gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg
     6481 ccctggtggg aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg
     6541 gtggtgaaga gagtgacgac ctgaatgccc gccaggttgc tggcgatatt tgctcgcgct
     6601 tttttgatat tcacggcgca atggttgaaa cgaatatgag cgaaaaaaca ctctctatcg
     6661 aaatgaataa attaaagcag gcacgatatt ccattggcat tgccatgagt gaagaaaaat
     6721 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
     6781 gcacagctga actattactg aaataaaaat cttcccgatg tttagcgtaa taaaaattac
     6841 ggcggggccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
     6901 ataaaatcat tattgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
     6961 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
     7021 aaggtcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
     7081 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
     7141 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
     7201 acgaagctgc attcgaaaaa atggtcaata tcaaccagaa aggcgttttt ctgatgtcgc
     7261 aggcggtggc gcgacagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
     7321 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctta
     7381 atagcttcac gcgatcctgg tcgaaagaat tgggtaaaca cggtatccgt gtggtcggta
     7441 tcgcgccggg gattctggaa aaaacaggac tgcgcacgcc ggaatatgaa gaagcgctgg
     7501 tgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
     7561 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
     7621 aacgcgccag ctatatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggct
     7681 aaggaggctt tatggtcaat gccatttttt gcgcccacgg caaattagct tgcgccatgc
     7741 tggaatcggt tcagatggtg tacggcgacg ccagggtcga ggcagttgaa tttgtgcccg
     7801 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
     7861 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
     7921 ttgcgatgcg caatcctcgt ctgcgggtaa tcagcggcct ttctctgccg ctggcgctcg
     7981 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
     8041 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggag gatttctgat
     8101 gaacattacg ctcgcccgca ttgatgaccg cctgatccac ggtcaggtca ctaccgtctg
     8161 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
     8221 agttcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
     8281 tattgaaaaa gccgtcgctg tttatcacaa cccgcaatat caggacgaaa ccgtttttta
     8341 tcttttcact cgcccacagg atgccttagc gatggttcgc caaggggtaa aaatagacac
     8401 cctaaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
     8461 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcatactgga
     8521 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
     8581 aatcgcaaat taaataatag cgcttgtgta catatgcttt aacaggcagg gatgacgaca
     8641 cttaaaaggt gaaaaatcat ggaaataagt accctacaaa tcattgctat atttcttttt
     8701 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
     8761 gcctgtacgg tgattagttt aattctcggt gatttaaaaa ccggaataat actcggtggt
     8821 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
     8881 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
     8941 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccagg tgctgactgt gtttgcccgt
     9001 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaagc acggtttcgc
     9061 actctcgata ttctgcatgt ctccgcgttt ggcgtgcagg cgctgcgcgt tgctattccg
     9121 gcactgattg tctcactgtt cgtcagcgcc gatatggtga gcaatatgct gagcgcgata
     9181 ccggaattcg tgacgcgcgg tctgcaaatt gctggcggtt ttatcgtggt ggtcggttac
     9241 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
     9301 ctcgcaggtg gctacctcga tctcagtctg ctggcgttcg gtggcgtcgg cgtgatcatg
     9361 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
     9421 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
     9481 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
     9541 taactttgaa cgtattcacg ggctgggttt ttgctacgac atgatccccg ccatcaaacg
     9601 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
     9661 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
     9721 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
     9781 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccaccgc
     9841 gctcggcgca tctctggcac tttcgggcaa cattctcggc ccgctgctgt tcttctttat
     9901 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
     9961 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    10021 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    10081 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcaccatga ccgtgcagaa
    10141 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctgc ttatggttcg
    10201 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    10261 tatcggtaat gcgctgggct tcctgtccag attcttcgcc ccggcatgac tgccggggcc
    10321 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    10381 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    10441 ggtcactgac agcctgtgcc tctcttcctg gaaagaggcc aatctgggtg aaaaccataa
    10501 aaaagtaccc gacgatgtgg cgaccaaccc catcatcatc ggccacgagt tttgcggcga
    10561 tattctggcc gtgggtaaaa agtggcagca caaattccag ccgggtcagc gttatgtgat
    10621 tcaggccaac ctgcaactcc ccgaccgccc ggactgcccc ggctactcct tcccgtgggt
    10681 gggcggcgaa gccacgcatg tggttattcc caacgaggtc atggaacaag attgcctgct
    10741 ggcatacgac ggcgaaacct attttgaagg ctcactggtt gaaccgcttt cctgcgtgat
    10801 tggcgcgttc aacgccaact atcatcttca ggaaggtagt tataaccaca cgatggggat
    10861 tcgcccgcaa gggcgcatgc tgatcctcgg cggcaccgga ccaatgggac tgttggcgat
    10921 tgattatgcg ctacatggac ccgttaaccc gtcgcagctc gtcattaccg ataccgacaa
    10981 cgataaattg agttatgcgc gcaagcacta tccatcagaa ccgcaaacac tgattcatta
    11041 tctcaatgcc gccgatgcag catttgatac gctaatggcg ctgagtggcg gtcacggctt
    11101 cgatgatatt ttcgtctttg tgcctaatga aggactggtg actctcgcct cttccttgct
    11161 ggcgacagat ggttgcctga atttcttcgc cggaccgcag gataaacatt tcagcgcgcc
    11221 aattaatttc tacgatgtgc attatgcatt tacccactac gtgggcacgt caggcggcaa
    11281 taccgacgac atgcgcgcgg cggtcaaact gattgaagag aaaaaagtgc aggccgcaaa
    11341 agtggtaaca catattcttg ggctgaatgc cgcgggcgaa accacgcttg aattgcctgc
    11401 cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac ctgccgctga cgtcactcac
    11461 gcagattcag gatcaagcac tggcggcgat tctggcgcgt catcagggga tctggtccgg
    11521 tgaggcggag caatatctgc tcactcatgc agaggcaatt tcccatgatt aatcgcgcta
    11581 cttaactctg catgtcgctg gctggtcgcc gcggcaactt cgcgacctga tgtgcggctg
    11641 attgctgaag cggcccaatg tgcacgagaa ggataaaaaa acatcagcgc cttaaggggc
    11701 gctcaaattg gtggcaaagt acacattgtt tatgccggat gcggaataaa gccttattcg
    11761 gcctacataa tctcgaaaat tcaatatatt gcagttatct ggtaggcctg gtaagcgtag
    11821 cgcatcaggc aatgctgtgt ttgtcatcag tctcagctcc cggtgcaata cgtgacatcg
    11881 tgagccatgt cagtaagtgg ttaaattcgc agtggtagca tgccttccgt gatttcttac
    11941 taaccggaga agtaatggaa ctgcttttat tgagtaactc gacgctgccg ggtaaagcct
    12001 ggctggaaca tgcactgccg ctaattgctg aacagttgca gggtcgccgc tcagcggtgt
    12061 ttatcccttt cgctggcgta acgcagacct gggatgatta cacagcgaaa acggctgcgg
    12121 ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat cccgttgccg
    12181 cgattgaaaa tgctgagatc gtgattgtcg gcggcgggaa tactttccag ttgctgaaac
    12241 agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt ggtgcgctgt
    12301 atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata
    12361 tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgttg caaatcaacc
    12421 cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc
    12481 gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa ggtaactgga
    12541 tcacagtgag taaaggtcac gctacgctgg gtggcccgaa caccacctat gtgtttaagg
    12601 ccggtgaaga agcggttccg ctggaagctg gtcaccgttt ttaagtgaat tgccggatgc
    12661 atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta
    12721 caggattcac aatattaata ctcgtcccgt ccttcatctt catccgactg ttccagcaca
    12781 ctgtaagcca ccgaacaaaa cagcgagttc aggcgctgca tatctcccag taagcctaaa
    12841 tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg
    12901 tgggaatagc ggcgattaag aatgcgaaag cgatgcttac tacggcgcaa acgacgagcg
    12961 ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc
    13021 tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagt
    13081 gatttgtcag caatttcgct gcccatgcgc tcgacgatat cggaggcctg ttcaaggttg
    13141 agcgacattt caataatctc cgcccagcgg cgcgactctt cctccgccag ctcttctttt
    13201 ggcatccgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc
    13261 ttacgcagct ctttctcctg ccgtggctca ccgtgcatca ctttattcaa cccttccatc
    13321 atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc
    13381 gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc
    13441 ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg
    13501 accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc
    13561 aatgacagct tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta
    13621 cccaccagct taaacagcag actacccagc gcgacacggc gggctgcggc attggcggca
    13681 ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc
    13741 gccacgggga aggagataat gcctgcggcg gtcagagccg cggtcagcag tacagcagca
    13801 aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg
    13861 ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc
    13921 tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt
    13981 tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac
    14041 ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct
    14101 gcccccagca caatgaccag agccggtgcg agggctacca gatcctgagc gacgaacgag
    14161 gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg
    14221 caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg
    14281 aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca
    14341 gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg
    14401 aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt
    14461 gacattcgtt tttccggcta agattattcc agcctgattc cagtataagt gtttcatatt
    14521 aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt
    14581 gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atctgagacg
    14641 ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccacggcg
    14701 tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc gggccacatg
    14761 gcgaaagatt ctgtgagttt catgccggta tgtttttcca cttccagcag ctcccagatg
    14821 gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg
    14881 tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag
    14941 actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc
    15001 gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg
    15061 tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc
    15121 ttcggcgcaa caaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga
    15181 cgcagatggt ggctgacatt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg
    15241 tcatcgccca cacggtttgc cgggaacagg cccaccacgc cacgcggatt cagcgttttc
    15301 tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg
    15361 cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag
    15421 aacggtgtcc agtcgatgca attacgcaga gtttcgatgc tggcttcaac ttcctgcacg
    15481 ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatcg
    15541 cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg ctgaatacgt
    15601 acggtttcgt actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc
    15661 agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg
    15721 tagttctgct caattttcac cgccgtgtgc gcttttgagg tggtcgcgcc accgatcagt
    15781 aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc
    15841 gacggcgtga taagccccga aaggccaatc aaatcagcat tcacttcttt agcagtacgg
    15901 agaatttttt ccgcaggcac catcacgccg agatcgacaa tttcgtagtt gttacattgc
    15961 agcaccacgc caacgatatt tttaccgata tcgtggacgt cgcccttcac ggtggcgatc
    16021 accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgagatac
    16081 gccaccgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttgcct
    16141 tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca
    16201 atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcggtaatg
    16261 cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc
    16321 tgggcgttat cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa
    16381 cgctcagtgc catcgtcacg acgattaaga atcacatctt ccaccgcgtc gcgcagttca
    16441 gcgggtaggt cgtcgtaaat cgccagttgc cccgcgttga cgatccccat atccatgcca
    16501 ttgcgaatag cgtagtagag gaacactgcg tgaatggctt cgcgcaccgg atcgttgcca
    16561 cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt
    16621 ttgatgtctt cacacgcgcc gataaagtcc tgcgcgtagt tgttgtgctc ttcaataccg
    16681 gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct
    16741 tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg agtatcggcc
    16801 tgtccctgtt catcaaaggc catcaccacc actgccgcac cgtagcgacg caacaatttc
    16861 gcgtgatgga taaaggcgtc gacgccctct ttcatcgaga ttgagttaac aatgcctttg
    16921 ccctgaatac acttcagccc tttttcgata acgtcccatt ttgaggagtc gatcataatc
    16981 ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct
    17041 tcggcatcga gcatcccttc atccatgttg atatcgataa tctgcgcgcc gttttccacc
    17101 tgctgacgcg ccacatcctg cgcctcgctg tatttctctt ctttgatcag gcgcttgaac
    17161 ttagcagagc cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcgtcgcca
    17221 atgttcagcg gctccaggcc agacaaacgg caggctacgg gaatttccgg cagtttgcgc
    17281 ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgcggcgt ggtcccgcag
    17341 caaccaccga cgatattgag aaagcccgct tgcgcccatt cacgtatctg ttttgccatc
    17401 gtgtcggcgt cgagatcgta ttcaccaaag gcgttaggca gcccggcgtt cgggtgggcg
    17461 gtgacgtagc attccgcaat ccgtgatagt tcctgcacgt actggcgcag ttcatcgggc
    17521 cccagcgcac agttcaggcc aaaggttagc gcttcggcgt ggcgcaatga gttgtaaaat
    17581 gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccggagatc
    17641 ataatcggca gctcaacgcc cagcgcttca aactccgttt tcaccgcaaa aaccgccgct
    17701 ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc
    17761 agcgctttgg tggattcgcg ataggccgcc accagctggt caaaagtgat gttgcggaat
    17821 gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc gagaacaccg
    17881 gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc
    17941 agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc
    18001 gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag
    18061 gcgttgtgga tagcggcgat cacttccggt ttgctgagca ccagcaggtc gttgttgcct
    18121 ttgagatcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga
    18181 taactctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc
    18241 gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta
    18301 ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc
    18361 aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattagcta
    18421 acaataaaaa tgaaaatgat tcccacgata cagaaaaagg agactgtcat ggtcgcaccc
    18481 attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt
    18541 cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg
    18601 gcactcacgg aactggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta
    18661 accacaatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc
    18721 ggcgcacatg cctttatggt cggcagcagc tttctccaga gtcgtaattt gttagcaatt
    18781 gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg
    18841 cttgatcaaa gcgatcacga agcgattatt atcgaccagg tacagtgtac gcatctgatg
    18901 cgaatgtccg cgcctatcgg cggtaaattg ccgatgcacg cttccggtgc aggtaaagcc
    18961 tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat
    19021 gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg
    19081 cgcaaacggg gttattcatt tgacgatgag gaacatgcgc tggggctacg ttgccttgca
    19141 gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt
    19201 tcacgtatta ccgatgaccg cgtgaccgag tttggcgcaa tggtgattaa agcggcgaag
    19261 gaagtgacac tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt
    19321 ctgcccatca ta
//
LOCUS       DADLTD010000065        19223 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLTD010000065 DADLTD010000000
VERSION     DADLTD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.065"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..731
                     /gene="rihB"
                     /locus_tag="JN701_001813"
     CDS             <1..731
                     /gene="rihB"
                     /locus_tag="JN701_001813"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY7364284.1"
                     /translation="MRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMAS
                     DGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPEA
                     ARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENY
                     GLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKV
                     GITIDTDWFWGLVEECVRGYIKTH"
     gene            831..2081
                     /locus_tag="JN701_001814"
     CDS             831..2081
                     /locus_tag="JN701_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7364285.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVFFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2137..3225)
                     /locus_tag="JN701_001815"
     CDS             complement(2137..3225)
                     /locus_tag="JN701_001815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7364286.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3228..4085)
                     /gene="nfo"
                     /locus_tag="JN701_001816"
     CDS             complement(3228..4085)
                     /gene="nfo"
                     /locus_tag="JN701_001816"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY7364287.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4159..5208)
                     /locus_tag="JN701_001817"
     CDS             complement(4159..5208)
                     /locus_tag="JN701_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY7364288.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5308..6189
                     /locus_tag="JN701_001818"
     CDS             5308..6189
                     /locus_tag="JN701_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7364289.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6394..7863
                     /gene="lysP"
                     /locus_tag="JN701_001819"
     CDS             6394..7863
                     /gene="lysP"
                     /locus_tag="JN701_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY7364290.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8070..10050
                     /gene="cirA"
                     /locus_tag="JN701_001820"
                     /pseudo
     CDS             8070..10050
                     /gene="cirA"
                     /locus_tag="JN701_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10082..10918)
                     /gene="yeiG"
                     /locus_tag="JN701_001821"
     CDS             complement(10082..10918)
                     /gene="yeiG"
                     /locus_tag="JN701_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAY7364291.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11176..11844
                     /gene="folE"
                     /locus_tag="JN701_001822"
     CDS             11176..11844
                     /gene="folE"
                     /locus_tag="JN701_001822"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY7364292.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11861..13018
                     /locus_tag="JN701_001823"
     CDS             11861..13018
                     /locus_tag="JN701_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY7364293.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13160..14200
                     /gene="galS"
                     /locus_tag="JN701_001824"
     CDS             13160..14200
                     /gene="galS"
                     /locus_tag="JN701_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY7364294.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14480..15478
                     /gene="mglB"
                     /locus_tag="JN701_001825"
     CDS             14480..15478
                     /gene="mglB"
                     /locus_tag="JN701_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY7364295.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15539..17059
                     /gene="mglA"
                     /locus_tag="JN701_001826"
     CDS             15539..17059
                     /gene="mglA"
                     /locus_tag="JN701_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY7364296.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVEAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17075..18085
                     /gene="mglC"
                     /locus_tag="JN701_001827"
     CDS             17075..18085
                     /gene="mglC"
                     /locus_tag="JN701_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY7364297.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18328..19223)
                     /gene="preA"
                     /locus_tag="JN701_001828"
                     /pseudo
     CDS             complement(18328..19223)
                     /gene="preA"
                     /locus_tag="JN701_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
BASE COUNT         4570 a         4760 c         5103 g         4790 t
ORIGIN      
        1 ttatgcgtaa acaaatcgtt gccgataata ttcacggtga aaccggactg gatggtccgg
       61 tattcgagtc gctgacccgc caggcagaaa gtacccacgc ggtgaaatac atcattgata
      121 ccctgatggc aagcgatggc gatatcactc tggtgccggt tggtccgctt tcaaatatcg
      181 cggtggcaat gcgtatgcaa cccgcgatcc tgcccaaaat ccgtgaaatt gtgctaatgg
      241 gcggcgctta cggtacaggc aacttcacgc catctgccga gttcaacatc tttgctgatc
      301 cggaagcagc ccgcgtggtg ttcacctccg gcgttccatt agtgatgatg ggcctcgatc
      361 tcaccaacca gactgtttgc accccggacg tgattgcacg gatggaaagg gcaggcggcc
      421 ctgccggaga gctgttcagc gacatcatga acttcactct caaaacgcag ttcgaaaact
      481 acggcctggc tggcggcccg gtgcacgacg ccacctgcat cggttatctg attaaccctg
      541 atggcattaa aacccaggag atgtacgtcg aagtggacgt caacagtggc ccttgctatg
      601 ggcgtaccgt ctgcgacgag ctgggcgttc ttggcaagcc cgctaatacc aaagtcggca
      661 tcactattga taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc ggctacatca
      721 aaacccatta agtgttgata acaagccggg tcagtatcgc aagatccaag cccggctatt
      781 tctgatgggc agaaagccgt cgcccaatat gacatgaaga gataataact atggatgtca
      841 tgagaagtgt tctgggaatg gtggtattgc tgacgattgc gtttttgctg tcagtaaaca
      901 agaagaagat cagcctgcgt accgttggcg cggcgttagt gttacaggtc gtgattggcg
      961 ggattatgct ttggttaccg gcagggcgtt gggtcgctga aaaagtcgct tttggcgtgc
     1021 ataaagtgat ggcgtacagc gacgcgggta gcgcatttat cttcggttcg ctggtcgggc
     1081 cgaaaatgga cacgctgttt gatggcgcag gatttatctt tggtttcagg gtattaccgg
     1141 caattatctt cgtcactgca ctggtgagta ttctctacta catcggtgtg atggggattt
     1201 taattcgcat tctcggcggt atattccaga aagcattaaa tatcagcaag attgagtcat
     1261 tcgtcgcggt caccaccatt ttcctcgggc aaaacgaaat tccggcgatc gtcaaaccct
     1321 ttatcgatca tctgaatcgc aatgaattat ttacagcgat ttgtagtggc atggcctcga
     1381 ttgctggttc gacaatgatt ggttacgccg ccctgggcgt acctgtggaa tatttgctgg
     1441 cggcatcgtt aatggcgatc ccaggcggga tcttgtttgc tcgcctgtta agcccggcta
     1501 cggaatcttc gcaggttttc tttaataacc tctctttcac cgaaacaccg ccaaaaagca
     1561 ttattgaagc cgctgcgaca ggggcaatga ccgggctgaa aatcgccgcc ggtgtagcga
     1621 cagttgttat ggcatttgtc gccatcattg cgttaattaa tggtattatc ggcggcgttg
     1681 gcggctggtt tggttttgca catgcctcgc tggagtccat tttaggttac ctgttggccc
     1741 cattggcgtg ggtgatgggg gttgactgga gtgatgcaaa tcttgccggg agtttgattg
     1801 ggcagaagct ggcgatcaat gaatttgtcg cttatctcaa tttctcgcca tatctgcaaa
     1861 cgggtggcac tctggatgct aaaaccgtgg cgattatttc tttcgcgttg tgcggtttcg
     1921 ctaactttgg ttctatcggg gtggtggtgg gggcgttttc tgcggttgcg ccacaccgtg
     1981 cgccggaaat cgcccaactt ggtttacgcg cgctggcggc ggcgacactt tctaacctga
     2041 tgagtgctac tattgcagga ttctttattg gtttagcgta gtgcgcaatt tatgagccgg
     2101 ataagatgcg gtcaagcgtc gaatccggca acattgttac accattggca ctaatgaaag
     2161 cgcgttatcg gcagacaggg tagggttgtt taagctgccg ctggcgcgcg aaattgccgc
     2221 gcaggctacc gcaaaacggg cgctgtcgcg gaaactgtat ccttcaagaa agctatatac
     2281 caggcccgcc ataaaaccat cgccagcacc aaaactgtct actgtcgtat gcgctggcgc
     2341 ggtcagcaaa aattgttctc catccttttc gctgcaataa actgactcat cgggcaaata
     2401 aacaaacagt tgctgaacac cttgctgatg caatgcattc actgcggcat tacggtcagc
     2461 atcgctggtg atcgcctgtc cccataaaat ttccagctcc ggtaaagtgg gtttcagggt
     2521 gtgaatatgc gccagccagt gtttgatttt gcccgctttg aattctgaaa cggtatcgac
     2581 aaacaccggg atttcatcag cgagggtaaa gacccattcc agcgcctcgg ctgtcaggtt
     2641 gcaatcagcc agtaccacgc ccgcatgacg aagtaaatcg cgcgacccgt tcagtagttg
     2701 cggtgtcaac tgttccagca gatgggtatc gttaatcgcc agcacggttt gatcgtctcg
     2761 attggcaatt gccagatacg tcgatgtgct ttgaccatgc aaacgaacgc agccggagac
     2821 attcacgccg gcacggcgcg tttcttccag gagcatttcg ccataaaagt catcgccaat
     2881 cactgaaagc aaatggacgt cacggcctaa cagcgccaga ttgtgggcga tgttgcgtcc
     2941 cacgccgccc gccgagcaat gaattgtacc gggatgagaa gccgcttgcg ggtaacggat
     3001 atccgcgatc ccgcgaatat ccatattgat tgtccccacc actacgcagt attcctgctc
     3061 ggtgagaatg taacctttgc ctttaatccg ccctttgcgc attaaatcca taatatgcgc
     3121 cgcaacgcgc gaacgactga tttgcagcat gtccgcaatt tcgttctgct gaatcagcgg
     3181 gttacgccgt aaaattgcaa ggatctcctt ttcccggtta ttcatcttca ggctaccgct
     3241 ttttcagttt gttgcgcttt cagccaggcg atctcttctg cccagatatc cgggttgatg
     3301 gtttcaagga tcagcggaat gccgtcgaaa cggtcgtcct gcatgatcca gcggaacgca
     3361 tcatgaccga tattgccttc accgaggcta tgatggcggt caacgcggct gccaaaggtg
     3421 cttttcgcat cgttaaggtg catcccgcgc agatacttaa agccaacaat acgggcaaaa
     3481 tccgcgaacg ttttctcgca ttcggctgga gtacgcaaat catacccggc agcgaaagca
     3541 tggcaggtat caatgcagac gccgacacgg gatttatctt ccacgccgtc gataatcgcc
     3601 gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac cggcggtgtt ttcaatcacc
     3661 gctgttacac cttgagtttt atccagcgca atgttgatgg attcggcaat acgcgcaagg
     3721 caatcctctt ctgaaatctg catcagatgg ctgccagggt ggaagttgag caaagaaagc
     3781 cccagttgtt cgcaacgctg catttcatct ataaaggcat cgcgcgattt ttccagagct
     3841 tcagtgaccg gatgtccgag gttaatcaga tagctgtcgt ggggaagaat ttgcgccgat
     3901 gtgtagtgat atttttcaca ggctgctttg aattcatcga tggtttgcgt cgtgagcggt
     3961 gcggcacgcc actgacgttg gtttttggtg aacaaggcaa acgcggttgc gtcgatttcg
     4021 gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac taacgtgcgc tccaatgtat
     4081 ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg gtaatgatag cgggttaaca
     4141 ggagcaagat gtagtggttt atgcgatgac gctttgaatc acatagttaa tcgcaccacc
     4201 accaacaatc agccaggcaa acagtaccag tgccatcagc agaggtttcg ccccggcttt
     4261 tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc atcgccattg ccagcaggaa
     4321 ggtatccagc gttaccagca tgttcaccac gctctgcggt aacaggtgga acgagttaaa
     4381 gatggcaacg acgatgaaca agatggcaaa ccacggaata gtgattttgc ttttctcgcc
     4441 gctgttcgcc ccagacagct gtttaacacg cgccgccagc aggatgagga acggagccag
     4501 catcatcacg cgcagcattt tggaaataac tgctgcgttt tccgcatccg ggctgatggc
     4561 atgacctgcc gccaccacct gcgccacttc gtgcacagta gaaccaatgt agataccgaa
     4621 agtttccgga ctaaaccatt gagacatcag cggatatatc gccgggtaga ggaaaatcgc
     4681 gacggtcccg aagataacaa cggttgcaac agccacggtt actttactgg cttccgcttt
     4741 cactaccggc tcagtcgcca gtaccgcggc agcaccacag atactgctac cggcaccgat
     4801 caaccagctg gtgtgcttat ccagaccaaa cactttctgc cccaggaagc aagccagcag
     4861 gaaggtactg gacagcgtca acacgtcaat gatgatccca ctgataccga catcggcaat
     4921 ttgcgagaac gtcagacgga agccataaag aatgataccc agacgtaata aatattgctt
     4981 ggcaaacagc acaccaccgt cacagctttt ccagatgtgc ggatagatgg tgttgcctaa
     5041 aaccatcccc aacaagattg cgagggtgag ggcactaaac ccggcacccg caaccgcggg
     5101 aatggaacca ccccacaggg cgaccccggt gataactgca ctcagggcta accccggaat
     5161 aaaatgccac agtgtacgat gttgtttctg taaggtgata ttcgtcataa ccctctcctt
     5221 ataaccgggc ataaggttac gactaactgg tttaaaaata aaattgatta tatatttata
     5281 attaatcttt ataagtggta agcgactatg cacatcaccc tccggcagtt ggaagttttt
     5341 gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa
     5401 tcagcagtga gcgcagcctt gaccgacctg gaagggcagc ttggcgtgca actgtttgat
     5461 cgcgtgggga aaagactggt tgttaatgaa cacgggcggc tgctctatcc gcgtgcgttg
     5521 gcattgcttg aacaggcggt tgaaatcgaa caactgtttc gcgaagacaa cggcgcgatt
     5581 cgtatctatg ccagtagtac catcggtaac tacattctgc ctgcagttat cgcccgttat
     5641 cgccatgatt atccgcagtt gccgattgaa cttagcgttg ggaacagcca ggacgtgatg
     5701 caagcggtgc tggatttccg cgttgatatt ggctttattg aaggaccgtg ccacagcact
     5761 gaaatcattt ctgaaccgtg gctggaagac gagctggtgg ttttcgccgc gccgacttcg
     5821 ccgttggctc gtggtccggt caccttagaa cagctggccg ctgcgccgtg gatcctgcgt
     5881 gaacgcggtt ccggcacgcg ggagattgtc gattatctat tgctgtcaca tttaccgaag
     5941 tttgagatgg cgatggaatt aggtaactcc gaggcaatca aacatgcggt gcgtcatggg
     6001 ttgggaatta gttgcctgtc gcgacgggtg attgaagatc aattgcaggc aggcacatta
     6061 agtgaagttg cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg
     6121 caaaaacacc tttccaacgc gctacggcgc tttctggact attgcgatcc cgcgaatata
     6181 ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc
     6241 ctcagcatgc attcgccaga aaagagattg gctgctttac ttataatccc agggcgatca
     6301 tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt gccaatcgtc tgctacaatc
     6361 gcgcctcatt tttaagatgg atagcatttt tgtatggttt ccgaaactaa aaccacagaa
     6421 gcgccgggct tacgccgtga attaaaggcg cgtcacctga cgatgattgc cattggcggt
     6481 tccatcggta caggtctttt tgttgcctct ggcgcaacga tttctcaggc aggtccgggc
     6541 ggggcattgc tctcgtatat gctgattggc ctgatggttt acttcctgat gaccagtctc
     6601 ggtgaactgg ctgcatatat gccggtttcc ggttcgtttg ccacttacgg tcagaactat
     6661 gttgaagaag gctttggctt cgcgctgggc tggaactact ggtacaactg ggcggtgact
     6721 atcgccgttg acctggttgc agctcagctg gtcatgagct ggtggttccc ggatacaccg
     6781 ggctggatct ggagtgcgtt gttcctcggc gttatcttcc tgctgaacta catctcagtt
     6841 cgtggctttg gtgaagcgga atactggttc tcactgatca aagtcacgac agttattgtc
     6901 tttatcatcg ttggcgtgct gatgattatc ggtatcttca aaggcgcgca gcctgcgggc
     6961 tggagcaact ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc gatgatcggc
     7021 gtagctatga ttgtcggctt ctctttccag ggaaccgagc tgatcggtat tgctgcaggc
     7081 gagtccgaag atccggcgaa aaacattcca cgcgcggtac gtcaggtgtt ctggcgaatc
     7141 ctgttgttct atgtgttcgc gatcctgatt atcagcctga ttattccgta caccgatccg
     7201 agcctgctgc gtaacgatgt taaagacatc agcgttagtc cgttcaccct ggtgttccag
     7261 cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag ttattctgac ggcggtgctg
     7321 tcagccggta actccggtat gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac
     7381 ggtaaagcgc cgcgcatttt cgctaaactg tcgcgtggtg gcgtgccgcg taatgcgctg
     7441 tatgcgacga cggtgattgc cggtctgtgc ttcctgacct ccatgtttgg caaccagacg
     7501 gtatacctgt ggctgctgaa cacctccggg atgacgggtt ttatcgcctg gctggggatt
     7561 gccattagcc actatcgctt ccgtcgcggt tacgtattgc agggacacga cattaacgat
     7621 ctgccgtacc gttcaggttt cttcccactg gggccgatct tcgcattcat tctgtgtctg
     7681 attatcactt tgggccagaa ctacgaagcg ttcctgaaag ataccattga ctggggcggc
     7741 gtagcggcaa cgtatattgg tatcccgctg ttcctgatta tttggttcgg ctacaagctg
     7801 attaaaggaa ctcacttcgt acgctacagc gaaatgaagt tcccgcagaa cgataagaaa
     7861 taagtttcct cccttccttg ctaagccctc tcaaccgaga gggctttttc aattccattt
     7921 ccctgacaaa tcagaggctg ttccggcttt ctgggatgat cacctgcata caaataagtc
     7981 caccgcgatg ctgccgtacg caaggggacg tgaagaaggt gtgagcgata acccatttta
     8041 ttttcgtagt tacctcatgg agatatggaa tgtttaggtt gaaccctttc gtacgggtca
     8101 ggctgtgttt gtccgctatt tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg
     8161 aaacgatggt tgtcactgca tcttccgttg aacaaaacct caaagatgca cccgccagta
     8221 tcagcgtcat tacccaggaa gacctgcagc gaaaaccggt acagaatctg aaggatgtcc
     8281 tcaaagaagt gcctggcgta caactgacga acgaagggga taaccgtaag ggcgttagta
     8341 ttcgtggtct ggacagcagc tacaccctga ttctcgtcga cggtaaacgc gttaactccc
     8401 gcaatgccgt cttccgccac aatgatttcg atctgaactg gatcccggtc gattccatcg
     8461 aacgtattga agtggttcgt ggcccgatgt cgtcgctata cggctccgat gcgctcggcg
     8521 gtgtagtgaa tatcatcacc aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg
     8581 ataccaccat tcaggaacat cgcgatcgcg gtgataccta taacggtcag ttctttacca
     8641 gtggaccatt aattgatggt gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg
     8701 aaaaggatga cccgcaaaac tcaacgacca ccgataccgg agaaatgccg cgtattgaag
     8761 gattctccag ccgcgacggc aatgtcgaat ttgcctggac accgaatcaa aatcacgatt
     8821 ttactgccgg atacggtttc gaccgtcagg aactcgctgg acaaaaaccg cctggaacgc
     8881 cagaactact ccgtcagcca taatgggcgt tgggattacg gcaccagcga actgaaatac
     8941 tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac ttccgaaagc
     9001 aatacggtcg acggcaaata cacgttgccg ctgacggcga ttaatcagtt tctcacggtt
     9061 ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg gggaaccagc
     9121 tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg gatcttcgag
     9181 ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg tgaacactgg
     9241 agtccgcgtg cctacctagt ttataacgcc accgacaccg taacggtgaa agggggctgg
     9301 gcgacggcat ttaaagcacc ttctctgttg caacttagcc ctgactggac gagcaattcc
     9361 tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac cagcgaaagt
     9421 tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggg tgaatccagc
     9481 gttaccgttt tccgtaacga tgtgaaagat cgtatcagca tcagccgtac gtctgacgtc
     9541 aacgctgcac cgggctacca aaactttgtt ggttttgaga cgggcgctaa cggacggcgc
     9601 ataccggtat ttagctacta caacgttaac aaagctcgta ttcagggcgt ggaaaccgaa
     9661 ctgaaaattc cgttcaacga tgaatggaaa ctgtcgatca actacaccta caacgatggt
     9721 cgtgatgtca gcaacggcga aaacaaaccg ctatccgatc tgccgttcca tactgctaac
     9781 ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt ttctggtcac
     9841 tataccgggc agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg ttataccatc
     9901 tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg
     9961 aaccttggcg acaaggatct cagtcgtgac gactacagct ataacgaaga cggacgtcgt
    10021 tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac cgggcggact
    10081 ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa gaggcgatga
    10141 agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac gctttctggc
    10201 gcgcggcttc tgccagtacc gcaggttgca actggtcggc aagaaactga tcattatcgc
    10261 cctgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc agcgcgcaac
    10321 tgtcccattc cagccatgca tttttgtcct cacctaaata gctgctaaac gctttgattc
    10381 cccacgggac gctgcacgga ttcacaattg gcgcaaaggc cgaaacgctg gtgtatttac
    10441 ccggattttt cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg
    10501 cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat
    10561 aatcatacat ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg
    10621 cgccctggcc taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt
    10681 ctggcatcac cagtacaatc cccagttctg ccgccacgcg ctgggcaccc gccttggtgg
    10741 tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt
    10801 gatcacgagg gggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt
    10861 cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc
    10921 gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg
    10981 agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc
    11041 ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata
    11101 atcaggctgt aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg
    11161 caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta
    11221 gttgcgcgag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg
    11281 cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg
    11341 gctgatgaca gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt
    11401 ttctccggtc tggattacgc caacttcccg aaaatcaccc tcattgaaaa caaaatgaag
    11461 gtcgatgaaa tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt
    11521 gttaccatcg atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg
    11581 tcaaaaatta accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg
    11641 acgcagcaaa ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcc
    11701 attgatgcgg tgcattactg cgtgaaagcg cgtggcatcc gcgatgcaac cagtgccaca
    11761 acaacgacct ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg
    11821 cgcgctgtgc gtcatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtaacgct
    11881 cgattttgtt cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg
    11941 gctaccaaag gcggcttatc tcaatcccgc ctggtacggc gctattacgc cgcaggatgc
    12001 atggacctgg gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct
    12061 gctgtttggt gcgggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg
    12121 gttaacgctg ttagtcttgc tgggttttat tcacggttta ttgttctggg acggcgatat
    12181 tctgctggct tacgggctgg tgggcttaat ctgctggcgg ctggtgcgcg atgcgccatc
    12241 ggtaaaaagc ctgtttaata caggcgtcat gctttatctg gtggggcttg gcgttttgct
    12301 gttattgggg ctgatttccg acagccagac cagccgcgcc tggacgccgg atgcatcggc
    12361 tattttgtat gaaaaatact ggaagcttca cggcggcgtt gaagcgatca gtaatcgtgc
    12421 cgatggtgtt ggcaacagtt tactggcact gggcgcacag tatggctggc aactggcagg
    12481 gatgatgctc attggtgcgg cgctgatgcg cagcggctgg ctgaaagggc agttcagctt
    12541 acgtcactat cgtcgtactg gttttgtgct ggtggcgatt ggggtgacca ttaaccttcc
    12601 tgccatcgcc ctgcaatggc agctggactg ggcgtatcgc tggtgtgcct tcttacttca
    12661 aatgccgcgg gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc tgttttatgg
    12721 cttctggccg caattgagcc gctttaagct ggtgcttgcg atcgcctgcg tcggacggat
    12781 ggcgctgacc aactatctat tgcaaacgct gatttgtacc atgctttttt accacctcgg
    12841 tttgtttatg cattttgacc gcctggagct gctggcgttt gttattccgg tatggctggc
    12901 gaatatcctc ttctctgtta tctggctgcg ttacttccgc caggggccgg tggaatggct
    12961 ctggcgtcag ttaactttgc gtgctgccgg aacggcaata tctaaaacat caagataacg
    13021 atctggatca cattcgttaa caaaacggct gtaaccgttt ccattgctgt gactcgattc
    13081 acgaagtcct gtattcagtg ctgacaaaat agccgccagc aagcagtcat ttactgcaat
    13141 ctcataacag gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg ctggcgtctc
    13201 tgtggcaacg gtttcccggg tgctcaataa cagcacgctg gtcagtgccg acacgcgtga
    13261 agcagtaatg aaagccgtga gtgagctgga ttatcggcca aacgccaatg cccaggcgct
    13321 ggcaactcag gttagcgaca ccattggcgt ggtggtgatg gacgtttctg atgcgttttt
    13381 cggcgcgctg gtaaaagcgg tggatctagt cgctcagcag catcagaaat acgtgctaat
    13441 cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt taattcgcca
    13501 gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac tggcgcaatt
    13561 tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt acgcccatcg
    13621 ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt tgctgaataa
    13681 cggtcatcaa cgtattggtt atctttcttc cagtcacggc attgaagatg acgccatgcg
    13741 taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg aaagctggat
    13801 tggcactggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac tgctggggcg
    13861 caatctacaa cttaccgcta tatttgctta taacgacaat atggccgctg gcgcactgac
    13921 agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg gtttcgatga
    13981 tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc ccattgcttc
    14041 aatggctaaa ttagccaccg aactggcctt gcagggggca gcaggcaata ttgatcctcg
    14101 tgccagccac tgttttatgc cgacgttagt gcgtcgccat tctgtcgcaa cgcgccagaa
    14161 tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa tctgtgagtg
    14221 atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaacg ttgtaacccg
    14281 tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac aattaaagcc
    14341 gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc ttcggcgttc
    14401 agtaacactt cattaactct actgccctgc cgagcattta tctcaagcac taccctgcat
    14461 aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg tgatggccag
    14521 catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa caatctataa
    14581 gtacgacgat aactttatgt ctgtagtgcg caaggctatt gagcaagatg cgaaagccgc
    14641 gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc agaacgatca
    14701 gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg ttgacccggc
    14761 agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg ttttcttcaa
    14821 caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg ttggcactga
    14881 ctccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg cggcgaatca
    14941 gggttgggat ctgaacaaag acggtcagat tcagtccgta ctgctgaaag gtgaaccggg
    15001 ccatccggat gcagaagcac gtaccactta cgtgattaaa gaattgaacg ataaaggcat
    15061 caaaactgaa cagttacagt tagataccgc aatgtgggac accgctcagg cgaaagataa
    15121 gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta tcgccaacaa
    15181 cgatgcgatg gcaatgggcg cggtagaagc actgaaagca cacaacaagt ccagcattcc
    15241 ggtgtttggc gtcgatgcgc tgccagaagc gctggcgctg gtgaaatccg gtgcactggc
    15301 gggcacagta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc tggcgaaaaa
    15361 cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca acaaagtggt
    15421 ccgcgtacct tatgttggcg tagataaaga caacctggct gaattcagca agaaataaga
    15481 ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt aaaattatat
    15541 ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg gtatcaacaa
    15601 gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc cacattctat
    15661 ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat gcctgtttgg
    15721 tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg atttccattc
    15781 cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa acctggtatt
    15841 acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag gcatgtttgt
    15901 cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg atattgatat
    15961 cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga tcgaaatcgc
    16021 caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt cttcgttaac
    16081 cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc gcggctgcgg
    16141 tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg aagttaccgt
    16201 attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga tggacaagat
    16261 catcgccatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag aaaacaagcc
    16321 gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt cgattcgcga
    16381 tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg tgggggcgaa
    16441 acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg gcaccattac
    16501 gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc acggatttgc
    16561 actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata ttggttttaa
    16621 ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg ataaatcgcg
    16681 gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacac cggggcatcg
    16741 gacgcaaatt ggttcgctct caggtggtaa tcagcaaaag gtgattattg ggcgctggct
    16801 actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg atgtcgaggc
    16861 gaagtttgag atctatcagt taattgccga actggcgaag aaaggcaaag ggattattat
    16921 tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg tcatgagcaa
    16981 tggtctcgtt tccggaattg tcgacacaaa aacaacaacg caaaacgaaa ttctgcgtct
    17041 tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata agaaaagttt
    17101 tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc tggcgattat
    17161 tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc tcacccagtc
    17221 atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc aggggaccga
    17281 cctttctgcg gggcgtcaag tagggctggc agcagtggtg gcagcgacat tattgcagtc
    17341 catggataac gccaataaag tgttcccgga aatggcgacg atgccgattg cgctggttat
    17401 tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga ttatcgctta
    17461 tctcaacgtt acgccgttca ttaccacgct cggcacgatg atcatcgtct atggcatcaa
    17521 ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca gtggcttctc
    17581 tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca tcaccttcta
    17641 cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct tcggtaagaa
    17701 catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca acgtcggcct
    17761 gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg ggatgttaga
    17821 agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc tggatgctat
    17881 cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg tgattggcgt
    17941 ggtgaccggg gtaattattt ttactgtcat caactatggc ctgacgtata tcggcgtaaa
    18001 cccatgctgg cagtacatca tcaaaggggc gattattatc ttcgccgtag cgctggattc
    18061 actgaaatac gcgcgtaaga aatgatgtga atgtggtccg cattcggtca taaacgcaca
    18121 taacatcatg atctgtgtgg ttttgtaggc cggataaggc gttcacgccg catccggcaa
    18181 caggagccaa atgccggatg caacgctggc gcgttttatc cggcctacaa taagctcaac
    18241 atactgattt gtatggtttt gtaggccgga taaggcgttc aagccgcatc cggcgacagg
    18301 aaccaaatgc cggatgtgac gcgggttcta caacgttacc gggtgttctt tctcgccttt
    18361 cttaaacttc acttccccga gctcaataca acccaccggg cagacgtgac cacaaagcag
    18421 acaacccaca catttctcgg tattacaatg cggtgtgcgg gttttctcgc tccattccat
    18481 cgcctggtga ccgccgtcgt aacaggaaat ataacagcgt ccacagccaa cacatttgtc
    18541 aagattgata cggggataga caatataact gcggtctaaa tcttccgccg ggacaatatt
    18601 gttattcgcc agacctacca tctcctgcaa cgaatcaaat ccctgatcgg cgagataatg
    18661 cgataacccg ctcgccatat cttctacgat gcgattgccg tactgcataa tgccggtggt
    18721 gacctgtaac gttgctgcgc cgagcaataa aaactccgcc gcatcctccc aggtttcaat
    18781 gccgccgata ccgctgattg ggaaatcgcg cagttctgga tgggtgcgca tttgctgaat
    18841 gaagcgcagg gcgatcggtt ttaccgcttt accggaatat ccggaaatac tcgatttacc
    18901 gttaacgatc ggcataccga ttttctgatt aagatcgata ttggtgatgg atttaacggt
    18961 gttaattgcc gcaatgccat ctgcgccgcc gcgcttcgcc gccagcgcca cttcgcacat
    19021 atcaccgata ttcggcgtca tcttcgccag cattggcagc gtggaaccgc gtttcaccgc
    19081 ccgacaatat ttttctacca gcaccggact ttgcccgaca tcgctaccca tcgcatgaga
    19141 agtcatttgc ggacaggaga agttacactc gatcatatcc gcgccagctc ctcccattgc
    19201 tgctcatttt cccccatgat cga
//
LOCUS       DADLTD010000066        19140 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLTD010000066 DADLTD010000000
VERSION     DADLTD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.066"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..909)
                     /locus_tag="JN701_001829"
     CDS             complement(<1..909)
                     /locus_tag="JN701_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7364298.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQAL"
     gene            1376..2608
                     /gene="mdtM"
                     /locus_tag="JN701_001830"
     CDS             1376..2608
                     /gene="mdtM"
                     /locus_tag="JN701_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HAY7364299.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2649..3929
                     /locus_tag="JN701_001831"
     CDS             2649..3929
                     /locus_tag="JN701_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HAY7364300.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNATLVGGCIGLILYLLSQLPALFPLSNF"
     gene            4045..5197
                     /locus_tag="JN701_001832"
                     /pseudo
     CDS             4045..5197
                     /locus_tag="JN701_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5207..5975
                     /locus_tag="JN701_001833"
                     /pseudo
     CDS             5207..5975
                     /locus_tag="JN701_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5972..6229
                     /locus_tag="JN701_001834"
     CDS             5972..6229
                     /locus_tag="JN701_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAY7364301.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            6183..7154
                     /locus_tag="JN701_001835"
     CDS             6183..7154
                     /locus_tag="JN701_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HAY7364302.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7222..8394
                     /locus_tag="JN701_001836"
     CDS             7222..8394
                     /locus_tag="JN701_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7364303.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8407..9063)
                     /gene="kptA"
                     /locus_tag="JN701_001837"
     CDS             complement(8407..9063)
                     /gene="kptA"
                     /locus_tag="JN701_001837"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY7364304.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENEMAKHNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADIDKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     "
     gene            9211..9883
                     /locus_tag="JN701_001838"
                     /pseudo
     CDS             9211..9883
                     /locus_tag="JN701_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9880..10341
                     /locus_tag="JN701_001839"
     CDS             9880..10341
                     /locus_tag="JN701_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364305.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10354..11526
                     /gene="iadA"
                     /locus_tag="JN701_001840"
     CDS             10354..11526
                     /gene="iadA"
                     /locus_tag="JN701_001840"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7364306.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11591..12502
                     /gene="hypT"
                     /locus_tag="JN701_001841"
                     /gene_synonym="qseD"
     CDS             11591..12502
                     /gene="hypT"
                     /locus_tag="JN701_001841"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7364307.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12495..12887)
                     /gene="iraD"
                     /locus_tag="JN701_001842"
     CDS             complement(12495..12887)
                     /gene="iraD"
                     /locus_tag="JN701_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY7364308.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12884..12967)
                     /locus_tag="JN701_001843"
     CDS             complement(12884..12967)
                     /locus_tag="JN701_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY7364309.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13559..14389
                     /locus_tag="JN701_001844"
     CDS             13559..14389
                     /locus_tag="JN701_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7364310.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWSFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14530..15303)
                     /gene="uxuR"
                     /locus_tag="JN701_001845"
     CDS             complement(14530..15303)
                     /gene="uxuR"
                     /locus_tag="JN701_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY7364311.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15518..16978)
                     /locus_tag="JN701_001846"
     CDS             complement(15518..16978)
                     /locus_tag="JN701_001846"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY7364312.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(17059..18243)
                     /gene="uxuA"
                     /locus_tag="JN701_001847"
     CDS             complement(17059..18243)
                     /gene="uxuA"
                     /locus_tag="JN701_001847"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY7364313.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKHKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18583..18787
                     /locus_tag="JN701_001848"
                     /pseudo
     CDS             18583..18787
                     /locus_tag="JN701_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19030..>19140)
                     /locus_tag="JN701_001849"
     CDS             complement(19030..>19140)
                     /locus_tag="JN701_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7364314.1"
                     /translation="GTSAVSLGLTANYARTGGQVTAGNVQSIIGVTFVYQ"
BASE COUNT         4473 a         4868 c         4800 g         4999 t
ORIGIN      
        1 taatgcctgc atttgctctg ccgaaagtcc ggtatatcgt tgtatccatt caggatcggc
       61 cccattctgc aacaacaatc ggaggaggct ccgttcccct tctactttcc cttctttcct
      121 ccccctctcc cttcccaaca gccatccatc attatgaatt cgctctgcaa ttgtcattat
      181 tcgctccctg tggtgtggca ttcgcctggt aagttcgctg ataaacgcct taaaacgcgc
      241 ttcatcgcca gtcagtaaaa tgtaatttaa cagcgcagtt atctggctgt cattagcaca
      301 ctctgtaacc agtaatacta ccagttggtc aataagcccc atcaaatcgc gctggcgaat
      361 atgcttttgg atcaactcca acagggcgac tctgcgatgc tgcacaatct cgtcgtctgg
      421 cacgacagtg acatccacca gcgggaacgc tgcgctataa agtttccgtg cggtagtcgg
      481 ggcggcaaat tcgtccagcc agcacaggga ccaggggtaa ggactacggc taccgtgata
      541 aaacagcatc gggataacca gcggtagcgg ccggcgttta tcatgctcga tatggcgctg
      601 catcaccgcc atggaatatc gcatcaggcg aaatgccata tgaatatcct cgcggctctg
      661 atgttcaatc actacgtaaa tataaccatc accttcacgg gtctttaccg accacagaat
      721 atcggagtgt agcgcccgca atttttcatc gacaaagctg gcggattcca gttttaagct
      781 gtcgagatcg cacagttcac gtaaatcttt gggtaagtgg atctccataa aatcccgcgc
      841 ggtgtcaggg tgagtgagaa aggatttaaa taatgcgtca tgtggcgtgc tggtcgtgaa
      901 gtttgtcatg gcgatccgtc acctgtagat aagatggccc gactttagcg actaacaaaa
      961 ccattctcac ccggcaattt ctcgttcccc gaggtgcttt ccaggcgtct gcaaacgata
     1021 catttttccg gaaacaggct cgcgttttac atttcctgta cccggcagat attccccttt
     1081 ccaccgcgct cttttgatta accgcaaatt ttaattacac ttaaggtgta tattttctat
     1141 gcaacccatc aattaaagag gtgtaatgtg ctgatgacta tttgtaatcg ttatacattc
     1201 tgacctggag tcagaaggta tttctctgtc tgtgtgttca caggcagtgt ggttgattac
     1261 atgaaattca gtacatttgc agtctcgttg cccttctcgc ctgcctttcg tcattaccga
     1321 cggtattgaa tttcgttttc cccgttgggg ttctccgaac aaggagttgt ttgttatgcc
     1381 acgttttttt gcccgccatg ccgccacgct gtttttcccg atggcgttga ttttgtatga
     1441 ctttgccgcg tacctgtcga cggatctgat ccagccgggg atcattaatg tggtccgtga
     1501 ttttaatgcc gatgtcagtc tggccccggc agccgtcagt ctctatctcg cgggcggtat
     1561 ggcgttacag tggctgctgg ggccgctttc cgacaggatt ggccgcaggc cggtgctgat
     1621 taccggggcg ctaatattta cccttgcctg cgctgcgaca atgttcacaa cgtctatgac
     1681 acagtttctt atcgcgcgtg caattcaggg caccagtatc tgttttattg ccaccgttgg
     1741 ttatgtcacg gtgcaggagg cgttcggcca gacaaaaggg atcaagttga tggcgattat
     1801 cacctccatc gtactgattg cgccgattat tggcccgctt tccggcgcag ctctgatgca
     1861 ctttgtgcac tggaaagtcc tttttgccat cattgcagtt atgggtttta tctcatttgt
     1921 tggcttactg ctggcgatgc cagagacggt gaagcgcggc gcggttccat ttagcgccaa
     1981 aagcgtcttt cgcgattttc gtaatgtctt ttgcaatcgg ctgttcctct ttggcgcagc
     2041 aaccatctct ttaagctata tcccaatgat gagttgggtg gctgtctcgc cggtgatcct
     2101 gatcgacgcg ggcagcttaa caacttcgca gttcgcctgg acacaggttc cggtgttcgg
     2161 cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt aaagatccga ccgaaccgcg
     2221 ctttatctgg cgtgctgtac ccattcaact ggtcggcctc gcgctgttga ttatcggcaa
     2281 tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc accagtttgt atgctttcgg
     2341 gattggtttg atcttcccga ccttattccg ctttacgctt ttttccaata acttaccgaa
     2401 agggaccgtc tccgcatcgc tgaatatggt gatcctgatg gtgatgtctg tctcggtcga
     2461 aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg tttcatctgt tagccgtcgt
     2521 ggcgggcgtt atcgtcgttt tcaccctggc aggattgctt aatcgcgtgc gccaacatca
     2581 ggctgccgag ctggcggagg agcggtgatt tttgcgcgat cctgccgtca ggctctattc
     2641 ttaacgttat gaataaactc attgaactca gacgcgccaa aatgttggcg ctctctttac
     2701 tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt gccgcccaat ttttgggtga
     2761 gcggcgtgaa ggcgattgct gaagcggcga tggtcggcgc gctggcggac tggtttgcgg
     2821 tggtggcgct gtttcgccgc gtgccgattc cgatcatttc tcgtcatacg gcgattatcc
     2881 cgcgtaataa agaccggatt ggcgaaaatc tcggtcagtt cgtgcaggaa aaatttctcg
     2941 atacccagtc gctggtggca ttgattcgac gtcacgaacc ggcgttgttg attggcaact
     3001 ggtttagtca gccggagaac gcccgccgcg ttggtcagca tctgttgcag atcatgagcg
     3061 gttttcttga actgaccgat gatgcgcgta ttcagcgcct gcttaagcgc gcggtccatc
     3121 gggcgattga taaggtcgat ctttccggaa ccagtgcgtt gatgctggag agtatgacca
     3181 aaaacgatcg tcatcaggtg ctgctcgata cgctgatcgc acagttgatc gcccttctcc
     3241 agcgcgataa atcgcgcaag tttattgccc agcaaattgt tcgctggctg gagagcgagc
     3301 atccgctgaa agccaaaatt ttgcctactg aatggctggg cgaacatagc gcggagttgg
     3361 tttctgacgc ggtgaattct ttgcttgatg atatcagccg cgatcgtgcg catcagatcc
     3421 gtcatgcgtt tgatcgcgcc acttttgccc tgatcgacaa gctgaaaaac gatccagaaa
     3481 tggcagcgcg agccgatgcc gtaaaaagtt atctgaaaga agatgaagct tttaaccgct
     3541 atctcagtga attgtggggg gatttacggg aatggctgaa agcggatatc aacagtgaag
     3601 attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg gtttggcgaa acgttaattg
     3661 ccgatgatgc cttgcgggca tcgttaaatg gtcacctgga acaagccgcg caccgcgtcg
     3721 cgcctgagtt ttccgcattc ctgacgcgcc acatcagcga tacagtaaaa agctgggatg
     3781 ctcgggatat gtcgcggcaa atcgagttaa atatcggcaa agatctgcag tttatccgtg
     3841 tcaacgctac gctggttggc ggttgtattg ggctaatttt atatttgttg tcgcagctcc
     3901 cggccttgtt ccccctcagc aatttttaga aatccatgaa aaactaagtg gctaaccgcg
     3961 agagagatca aataaaccac actaaaagaa tggtaataat gtggcctctt ttcattatct
     4021 cccgtggtac ggggaaggaa aatcatgtca cttatcaccg atctacccgc cattttcgat
     4081 cagttctctg aagctcgcca gaaaggcttt ctcaccgtca tggatctcaa ggagcgcggc
     4141 attccgctgg ttggcactta ctgcaccttt atgccgcaag agatcccgat ggcagccggt
     4201 gcggttgtgg tttcgctctg ttctacctct gatgaaacca ttgaagaagc ggagaaagat
     4261 ctgccgcgca acctctgccc gctgattaaa agcagctacg gcttcggcaa aaccgataaa
     4321 tgcccctact tctacttttc ggatctggtg gtcggtgaaa ccacctgcga cggcaaaaag
     4381 aaaatgtatg aatacatggc ggagtttaag cccgttcatg tgatgcagtt gccgaacagc
     4441 gttaaggacg atgcctcgcg tgcgttatgg aaagccgaga tgctgcgctt gcaaaaaacg
     4501 gtagaagaac gttttgggca cgagattagc gaagatgctc tgcgcgatgc cattgcgctg
     4561 aaaaaccgcg aacgtcgcgc actggccaat ttttatcatc ttgggcagtt aaatcctccg
     4621 gcgcttagcg gcagcgacat tctgaaagtg gtctacggcg caaccttccg gttcgataaa
     4681 gaggcgttga tcaatgaact ggatgcaatg accgcccgcg ttcgtcagca gtgggaagaa
     4741 ggccagcgac tggacccgcg tccacgcatt ttaatcaccg gctgcccgat tggcgacgca
     4801 gcagaaaaag tggtgcgggc gattgaagag aatggcggct gggttgtcgg ttatgaaaac
     4861 tgcactgggg cgaaagcgac cgagctaatg cgtggcggaa acgggcgatg tctacgacgc
     4921 gctggcggat aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg atcagcgcct
     4981 gcaaatgctc agccagatgg tggaagaata tcaggtcgat ggcgtagttg atgtgatttt
     5041 gcaggcgtgc catacctacg cggtggaatc gctggcgatt aaacgtcatg tgcgtcagca
     5101 gcacaacatt ccttatatcg ctattgaaac agactactcc acctcagatg tcgggcagct
     5161 cagtacccgt gtcgcggcct ttattgagat gctgtaagga gtggcagtga catattcgat
     5221 tggcattgat tctggttcta ccgccaccaa agggatctta ctggcagacg gcgtgattac
     5281 gcgccgtttc ctcgttccaa caccctttcg tccggcaaca gcaattactg aagcctggga
     5341 aactctgcgc gaagggttag agacaacgcc gtttctgacg ctcaccggct atggacggca
     5401 actggtggat tttgccgata aacaagtgac ggaaatctcc tgtcacgggc tgggcgcacg
     5461 gtttcttgcg ccagcaacgc tgcgcggtaa tcgacatcgg tggtcaggac agcaaagtga
     5521 ttcagcttga tgatgacggt aacctgtgcg atttcctgat gaatgacaaa tgcgcggcgg
     5581 gcaccgggcg tttcctggaa gtgatctcgc gcacgcttgg caccagcgtc gagcaactcg
     5641 acagcattac cgaaaatgtc acgccgcatg ccatcacgag tatgtgcaca gtgtttgccg
     5701 aatcagaagt gatcagcctg cgctcagcgg gcgtcgcgcc agaagcgatt ctcgccggag
     5761 tgattaacgc gatggcgcgg aggagtgcca atttcattgc tcgtctctcc tgtgaagcgc
     5821 cgattctgtt tactggtggc gttagtcatt gccagacgtt tgcccggatg ctggaatctc
     5881 acctgggaat gccggtaaat acccatcctg atgcgcaatt tgctggcgca attggcgcgg
     5941 cggtaattgg tcaacgagtg aggacacgcc gatgaaagag tttttatttt tatttcactc
     6001 cacagtcggc gtcatacaaa ctcgcaaagc gttgcaggca gcgggcatga cttttcgcgt
     6061 cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta tgcatttggc tgacctgtcc
     6121 ccccggcgaa gaaatacaat gggtgatccc tgggctgact gagtcaattt attgccagca
     6181 ggatggtgtc tggcgctgca ttgcacatta ccgggtttct ccgcgttaag gattcattaa
     6241 ggtcgctacg tatacttctc gatatcttat tgatttgcct taaagggatt accgtgacaa
     6301 aaagtatttc gttaagtaaa cgcatctccg ttattgtgat tctttttgcc attgtggcag
     6361 tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa tcatgcagca tcatttcaga
     6421 attaccacgc caccattgac ggcaaagaaa ttgcaggcat aacgaataat atctcttcgc
     6481 tgacctggtc ggcacaaagc aatactcttt ttagcactat caacaaacct gccgccattg
     6541 ttgaaatgac caccaatggt gattttatcc gcacaattcc tctggatttt gttaaggatc
     6601 tcgagacgat cgaatacatt ggcgataatc agtttgtcat tagcgatgag cgcgattacg
     6661 ccatttatgt gatttcactg accccaaact cggaagtaaa aatcctcaaa aaaataaaaa
     6721 tcccgctgca agactcccca accaattgcg gttttgaagg gttagcttat tctcgccagg
     6781 atcacacttt ctggtttttt aaagagaaaa atccgattga ggtctacaaa gttaacgggc
     6841 tgttaagtag caatgaatta cacatcagca aagacgaggc actacaacgg caatttactt
     6901 tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa tacgcttctg gtgctgtctc
     6961 atgaatctcg cgcactccag gaagtaaccc tggttggcga ggttatcggt gaaatatcac
     7021 taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca ggccgaaggg attgcgatgg
     7081 atgcctctgg taatatttat atcgttagcg aaccaaatcg tttttatcgc tttaccccta
     7141 aatcatcgca ttaacaggct ggcatatttc acctttttgt tattaacttg ttaacaacaa
     7201 aaatgacata caaggttaaa catgccttcg tcctcgcatc ccgtagaacg cttttctttc
     7261 agcaccgcgc ttttcgggat gctggttctg accctaggta tgggattagg tcgcttcctt
     7321 tatacgccta tgttgcccgt cattatggcg gaaggggcgt tttcatttag ccagctctcg
     7381 tggattgcca gcggcaacta tgcggggtat ctggctggca gtctgctatt ttcgtttggc
     7441 gcatttcacc agccgtcgcg cctacgccca tttctgttgg tttctgccct ggcgagaggg
     7501 ttgttgatcc tcgcaatggc atggttgccg ccatttattc tggtgttatt gattcgcttc
     7561 ctggcgggtg tcgccagcgc cggtatgctg atttttggtt cgacgctgat tatgcagcac
     7621 acgcgccatc cttttgtgct ggcagctttg ttttctggcg ttggcattgc actgggcaac
     7681 gaatatgttc tggcaggcct gcattttgcc ttctcctcgc aaacgttatg gcaaggcgcc
     7741 ggggcgcttt ctggcatgat gctgattgca cttacgcttt taatgccctc gaaaaaacac
     7801 gccatcgcac caatgccatt ggcaaaaaca gagcaacaga taatgagctg gtggttactg
     7861 gctattctgt acggccttgc gggatttggt tatatcatcg tcgcgactta cctgccgctc
     7921 atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt agtcggctta
     7981 tcaattgtgc caggctgctt tggctggcta tgggcagcaa aacgttgggg ggctttgcct
     8041 tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc tgcttactct cgccagcgac
     8101 tctcctctct tgcttatcat tagtagtatc ggttttggcg gtacctttat gggcacgaca
     8161 tctctggtga tgactatcgc ccgccaactg agcgttccgg gaaatctcaa tcttttaggc
     8221 tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac cagtatgctc
     8281 ggcaatggaa catcggcgct tgccggcgcc actctctgcg gcgcggcggc gttgtttatt
     8341 gctgcgctta tcagcacagt acaattattt aagctgcaag tggtcacttc atgaggagtg
     8401 tttcccttac cactcaagaa attcgaccgc aaccgttgat gtcagccaga ctccgttttc
     8461 cgcttgccag aaaggaatgc ctcgtttcgc catttcctgt gctttgacgg ttaagacaac
     8521 cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag agagatgcac
     8581 gtaatgacga tcccctgcaa tcagcccctg tttttttatc tcgtcaagga aacggcttgc
     8641 cgtaccgtgg tagagaaact gtggcggtgt tttttcggca aaggaaatag cgacctgcga
     8701 agtcgaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac taaaacgctt
     8761 tttatcgctg gttgcgacca cggtatccag caatgcacgg gttaatcgct tgcccgcttt
     8821 ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcca gtacgatacc
     8881 aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat ccgccagctc
     8941 tttttcgttg tgctttgcca tctcgttttc gtccctggat tttctgtttt tatttaccaa
     9001 tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgcaga accttttcca
     9061 cacagcgtaa tgtttcccct atgcaaaaaa cgcatagcca gaaacatact tgcattttcc
     9121 ccatacccac atggctgata aggtttttca gttccaggaa ttttcctggc tcaagcaaca
     9181 cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc tgtagccggg
     9241 gagctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct caccataatc
     9301 tttttttccg gtgtttttca gctggtggcg cgtttttgat ttttccgttc tgaacggatc
     9361 gtttgggcaa ctgcctggtg cgaacggagc caccacctca tttcgcggcg cgggtggtgc
     9421 gggggctaaa gatggctttc tctttgcgct ggagctggca ccgtcagtca ttctctctct
     9481 ggggattatt tcaattaccg atggtcttgg tggattacgc gccgcgcagc agttaatgac
     9541 acctgtgcta aaaccgttgt tgggcattcc agggatctgc tcgctggcgc tgatcgctaa
     9601 cttgcaaaac actgatgccg ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat
     9661 taccgaacgc gataaagtga ttttcgccgc atatcaaacc agcggtagcg ccatcatcac
     9721 caactacttc tcttccggcg tcgcggtgtt cgcctttctg ggcacgtccg taatcgtacc
     9781 tttagccgtc atcctggtgt ttaaatttgt cggagccaac attttgcgcg tctggcttaa
     9841 cttcgaagaa cgtcgcaacc caacgcaagg agcgcaagca tgacaactca ggtccgcaaa
     9901 aacgtcatgg atatgtttat cgatggcgcg cgccgtggct ttactatcgc cacgaccaac
     9961 ctgctgccaa acgtggtgat ggcgtttgtc atcattcagg cgctgaaaat taccggcctg
    10021 ctcgactggg tggggcatat ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa
    10081 gcggcaaccg tccttttagc cgcattaatg agtatggggg gcgcagtcgg tgttgccgcc
    10141 agtctggcaa ccgctggcgc attaaccggt cacgatgtca ccgttttact gcccgcgatg
    10201 tatttaatgg gcaacccggt gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat
    10261 gccaaatatt acccgcatat cattacggtc tgcgtgatta acgcattgct gtcgatctgg
    10321 gttatgcagc ttattgttta acaaggagtt accatgattg attataccgc agccggtttt
    10381 accctgctgc agggagcgca tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc
    10441 gtcgctaacg gcaaaattat cgccgttgcc agcaatatcc cttctgacat tgtaccgaac
    10501 tgcacggttg tcgatctcag tgggcagatc ctctgcccag gttttattga tcaacacgtc
    10561 catttgattg gcggtggcgg cgaagcaggt cccacgacgc gcacgccgga agtggcgcta
    10621 agtcgcctga cggaagcggg cgtcacgtca gtggttggtc tgctgggcac cgactctatc
    10681 tctcgccacc cggaatccct gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc
    10741 gcctggatgc tgaccggcgc ttatcatgtc ccttcccgca ctattacggg ttccgtggaa
    10801 aaagacgtgg cgattatcga tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt
    10861 tctgccgcac cggacgttta tcacctggct aatatggcgg cagaatcccg cgttggcggt
    10921 ttgctcggca gtaaacctgg cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta
    10981 cagcctattt atgacctgct ggaaaactgc gatgtgccga tcagcaagct gctgccgacc
    11041 cacgttaacc gcaacgtacc gttgtttgag caggcgctgg agttcgcgcg caaaggcggc
    11101 accatcgata tcaccagcag cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc
    11161 gccgttcagg cgggtattcc gctggcacgc gtcaccctca gctccgacgg caacggtagc
    11221 cagccgttct tcgatgacga agggaattta acccatatcg gtgttgcagg ttttgaaacg
    11281 ttgctggaaa ccgtgcaggt gctggtcaaa gactatgatt tcagtatcag cgatgccctg
    11341 cgcccgctca ccagtagcgt agccggtttc cttaacctga gcgggaaagg cgaaattctg
    11401 cctggcaatg acgcagactt actagtcatg acgccagaat tgcgcattga gcaggtatat
    11461 gctcgcggca aactgatggt caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg
    11521 gcttaaacgg ttgtcagccg ccctaatgga cgtagaatgc ccgcaagggc ggctgacaga
    11581 gtaaaacgta atggatgact gttgtgcggt tttgcataat attgaaacca aatggcttta
    11641 tgattttctg accctggaaa aatgccgcaa tttttcccag gcggcagtca gtcgtaacgt
    11701 ctcgcaaccg gcattcagcc gccgcatccg cgcgctggaa caggcgattg gcgttgaatt
    11761 gtttaaccgc caggtgacgc cgctgcaact ctcagaacaa gggaaaatct tccattcgca
    11821 gatccgccat ctgttgcaac agttagaaag taaccttgca gagctgcgtg gcggcagcga
    11881 ttacgcgcaa cgtaaaatca agatagccgc tgcacactct ctttccctcg gtctgttacc
    11941 gtccattatc agccagatgc cgccgctctt tacctgggcg attgaagcta ttgatgtcga
    12001 tgaagcagtc gataaactgc gtgaagggca aagtgactgt attttttcct ttcacgacga
    12061 agatttgctg gaagcaccgt ttgaccacat tcgcttattt gaatctcaat tgttccccgt
    12121 ttgcgccagt gacgaatacg gagaagcaat ttttaacctc gcgcagccac actttccgtt
    12181 actgaattac agccgcaact cctacatggg gcgattgatt aatcgcaccc tgacgcgcca
    12241 cagcgagtta agtttcagca ccttttttgt ctcttcgatg agcgagctat taaagcaggt
    12301 tgccctcgac ggctgtggga ttgcctggct gccggagtat gccatccaac aagaaattcg
    12361 cagcgggcaa ctcgttgtgc tgaatcggga cgaactggtg atcccgattc aggcctacgc
    12421 ataccgtatg aatacccgca tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga
    12481 gctggagatt gtgcttagct gacattctca agcgtcgcac tgcgccaacc gccattatcc
    12541 cagaccagat caaacgttag cggttcatcg tcgtggtaca gcattatctc aaggctgatc
    12601 gccagagtgt ggcagtacgc ttctttcaaa ttaacctgca acgccgcaat ccgtggctca
    12661 tgaaataaca gtgcagaacg cagctgacgg caataccagc tctggagatg tttgtcgctc
    12721 gggttatgca cttgccactc gggcatgcct gaaacaagta atgacggtaa tgccgaatga
    12781 ggagaattaa aaagagtgag gagatcgctg cataccgatt tacgcagcaa tgacgtttta
    12841 ttgcctgaaa tttcaggtaa aacagcctga agtgattggc gcatcatttt gcgcactcct
    12901 gacgtttagc aacatatgcg gcttgccctg aacaccgggc accactgtat tgatgctcat
    12961 tttccatagc cattcccttt tgttcattac atccatgtca gtctgagaca ggcctgaatc
    13021 ctgtcagcaa agtataaaac cttacttgcc tatgaatatc tactgacttt tcaggaaaaa
    13081 agtcatgatt tgataaaaat catcaaactc aaaaaaaaac cgctagtata ttctgaatat
    13141 tccgaatagt tacgtagatg aaaatacttg tcaccgccgc tacttaattc cggtgagaaa
    13201 ttatttattt ccgcataatc agccatgtat tatttgaaac aattagtttg taaataatat
    13261 aaattgataa catatcaaat acatcaaaaa taccattcat aggcattatt aatatagaca
    13321 tcaatatagc aacatcaaat tccaattagt aataagatat gctttttaat tgcatgaaaa
    13381 taatttcaaa tgagcgtttt ttgatacttt agagtgtttc ctacccttta atatcaccgt
    13441 gtagagttga tatcactaca acagaaacat gcatgtcatt tttttacttc ttttttacta
    13501 cagaaaaatt tatctatttt tctaaacatc ggtgaaatac tcataaaagg taactattat
    13561 gtctatgcca ttaagcaatg cccttcagag tcaaattatt acagataatc acttccttca
    13621 tcatccaaaa gtcgacaata agttaacacg tgaatatgaa tatacacgca tggatacgga
    13681 aaatatctat cttctgcctc ttactcgtgg aaataaccat aattacgatg gaaaaagcgt
    13741 tgtcgaaatt agaaaactta atattggcaa acagtcctgg tcatttaatt atgcgacaga
    13801 tacatgcaga gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa
    13861 aatcacctca gctttagacg aaatatatgg tggcatatgt aaaaaggcgc atgctgctac
    13921 agagttagca gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga
    13981 ggactataca gttcatgaaa taacattagg ccctggttac aatgttcctg gttatgccgg
    14041 aaccacgata ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga
    14101 gcagccaaga attgatatct atattgatca gataataact gtctcagggg tggcaaattc
    14161 ttccggattc gcccttgccg cattacttaa tgctaatatt ggaatgggaa atgacccaat
    14221 aattggtata gaagcctatc ctggtaccgc ggagatccat gcaaaaatgg gatataacgt
    14281 tatccccggt gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc
    14341 agaactgttt gaattaaaaa atggtgaatg gaattatatt ggcaaataac cactgagaag
    14401 aatgagtact aacattcagg ttatatagat atttaaacaa taccctgcaa tttacaaagt
    14461 aatactcatt gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat
    14521 cataatcagt caggcgtcga ctttatccag cggccatgaa tcaaacagat agccgtcaaa
    14581 ataaatatcg tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg
    14641 ccacattgcc agcttcgccg ctcgggcatc ttttttgatt aacgcggcaa ggatctgttt
    14701 gtgatcgccc aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat
    14761 ccacattgga ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct
    14821 gttatgcgtt gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc
    14881 cggcgcgctg gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat
    14941 atcttcgcgg gtagcctgca gggcggcaaa ttccgcgatg ttgctctcaa gaagctgtcg
    15001 cgcctgcaac aattcaaagg ggccggcatc attgcagacg ttggcatccg gactgtctgt
    15061 gttgtggctg cctgagctat caagaacata gataccggca ccccggcgca cttccaccag
    15121 ccctttgatc tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat
    15181 ttctgcaatt tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat
    15241 cagatcgcgg atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tggcagattt
    15301 catggtgttg tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc
    15361 cttatccggc ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag
    15421 cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag
    15481 tttgattgac cagtaagtct atagggtaat cagttagtta cagcgcagcc acacactcgc
    15541 gcgcaccgcg ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat
    15601 tctgcggcag atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg
    15661 cgccctgata ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa
    15721 tggcattacc ctgctcatcc acgccctggg tgtaacgcat ccagccagcc acgcccagcg
    15781 ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agcagacgct
    15841 gcggtaactt ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt
    15901 tgctgaaacg ctcgatcagc agcgtcgcat aggccttcag gtccgtaccc tccggcatag
    15961 acagcgttgg cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt
    16021 tagtcatggt gtcggcaata gtttcatagc cgccgaggta accgaggtat gccaggaaag
    16081 agtggctgcc gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga
    16141 actgtgcgcc cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc
    16201 actgacggaa cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa
    16261 tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga
    16321 aggtgacgtt ctcttcaatc catgccgcca gctgcgggtc acgtgcctga gccagcccca
    16381 gtaccgcgac cttcgccaca tgaccgtttt cacgcacgtt atcgcaggac atcaccgtaa
    16441 atgctttcag ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg
    16501 acttcggcgc agtcgggttt tctagatcgt gcttgatcag cgggttattg agatccagtt
    16561 gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg
    16621 tttgcggacg cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg
    16681 cttctttcat cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg
    16741 tgtacagcag ttgctgtttt ttcaggtttt cgatcagcac gcggtcgttg cctggcatca
    16801 ggttaacttc gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca
    16861 gcgcctgatg tgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac
    16921 gagaatgatc ccatgacgga cgggcaaccg gcagattgct gtcaacaata gtagtcatcg
    16981 taaactccag aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg
    17041 cagccatgcc ggtggatatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc
    17101 gcgaacttcg gccaggcctt tcagacgacc aattgcggag taacctgggt tggttttctt
    17161 cttcaggtcg tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt
    17221 gccttccgct ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc
    17281 aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt
    17341 ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc
    17401 gttgtcagca cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt
    17461 tacggtatca accatccact gcatatcttc aatggtggaa acaatgcgcg gcaggccgag
    17521 gatcgggcgc ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac
    17581 tggaataatc gctttcagga agacggcaaa gttttcgcgc agcttcgctt tatcgatatc
    17641 tttgtacagc tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg
    17701 cagaccggca atgatgttac gcgtcagacg cgctttgtct tcatcgctca tagtggcgaa
    17761 gcgttcagct gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt
    17821 caggatatgc atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc
    17881 gtctggcagc acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta
    17941 gcacacggtg cgaatgccgc actgcgccag gttgcgcaga gtctgctgat agttagcgat
    18001 ccactgctca tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac
    18061 gacagaccac accaggcctg cgtcttcaac gatcgccttg tgtttgagga tctcttctac
    18121 ggaccatact tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc
    18181 ctgacggaca tcagctaaag aaaccagatc gtttgggccg taccagcgcc aggtctgttc
    18241 catgattttt cctctttcac atagtgggtt ccacagcaaa gagacgcgga accctggagt
    18301 tgtttaggta tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg
    18361 cgtataaatt ctaccttttg gtaagatata tcaacccaaa tcctgaaatt ggttaaccaa
    18421 atcacaagaa tttcactctc atggctgttg ctgcaaattg cagcaaaagg taatcgatgg
    18481 ttattgatca gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt
    18541 cgcccaaaat aacaacggtt caaccaggta aggtaaaacg ttatgcatgt gcttaacatt
    18601 ctctgggtag tattcggcat tggtctgatg ctggtactga caacgaccgc aggttcagac
    18661 acattcactg cattagcaat gcctcttaga ttattttgac ctgtcagtaa aagacacgtt
    18721 gaaaacctgg ggtctgctgg aactggtcaa ctccgtggtt gggctgatta ttagcaaggt
    18781 agcgtaaaag aacaccagag cctgccaatg gcaggctctg actgatgaca aagtctaaaa
    18841 atgtgcccgg acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc
    18901 agcaaagtgc gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa
    18961 gggctaacgt gcaggttttt agcttcaggt aatattgcgt aactgcatta gcaatgccct
    19021 gtgatttctt tattgataaa caaaagtcac gccaataatc gattgcacat tccctgcagt
    19081 cacctgccct ccggtacgtg cgtaatttgc cgttaatccc agacttaccg ccgaagttcc
//
LOCUS       DADLTD010000067        18979 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLTD010000067 DADLTD010000000
VERSION     DADLTD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.067"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(151..699)
                     /gene="pgsA"
                     /locus_tag="JN701_001850"
     CDS             complement(151..699)
                     /gene="pgsA"
                     /locus_tag="JN701_001850"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY7364315.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(756..2588)
                     /gene="uvrC"
                     /locus_tag="JN701_001851"
     CDS             complement(756..2588)
                     /gene="uvrC"
                     /locus_tag="JN701_001851"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY7364316.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(2585..3241)
                     /gene="uvrY"
                     /locus_tag="JN701_001852"
     CDS             complement(2585..3241)
                     /gene="uvrY"
                     /locus_tag="JN701_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY7364317.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            3536..3712
                     /locus_tag="JN701_001853"
     CDS             3536..3712
                     /locus_tag="JN701_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364318.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            3699..3923
                     /locus_tag="JN701_001854"
     CDS             3699..3923
                     /locus_tag="JN701_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY7364319.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3990..4712)
                     /gene="sdiA"
                     /locus_tag="JN701_001855"
     CDS             complement(3990..4712)
                     /gene="sdiA"
                     /locus_tag="JN701_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HAY7364320.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(4942..5694)
                     /gene="yecC"
                     /locus_tag="JN701_001856"
     CDS             complement(4942..5694)
                     /gene="yecC"
                     /locus_tag="JN701_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY7364321.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(5691..6359)
                     /gene="tcyL"
                     /locus_tag="JN701_001857"
     CDS             complement(5691..6359)
                     /gene="tcyL"
                     /locus_tag="JN701_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY7364322.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(6374..7360)
                     /gene="dcyD"
                     /locus_tag="JN701_001858"
     CDS             complement(6374..7360)
                     /gene="dcyD"
                     /locus_tag="JN701_001858"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY7364323.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(7465..8265)
                     /gene="tcyJ"
                     /locus_tag="JN701_001859"
     CDS             complement(7465..8265)
                     /gene="tcyJ"
                     /locus_tag="JN701_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7364324.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQGLVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(8353..8904)
                     /gene="fliZ"
                     /locus_tag="JN701_001860"
     CDS             complement(8353..8904)
                     /gene="fliZ"
                     /locus_tag="JN701_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY7364325.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(8950..9669)
                     /locus_tag="JN701_001861"
     CDS             complement(8950..9669)
                     /locus_tag="JN701_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY7364326.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(9989..11695)
                     /locus_tag="JN701_001862"
     CDS             complement(9989..11695)
                     /locus_tag="JN701_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY7364327.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            11961..13367
                     /gene="fliD"
                     /locus_tag="JN701_001863"
     CDS             11961..13367
                     /gene="fliD"
                     /locus_tag="JN701_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY7364328.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            13392..13802
                     /gene="fliS"
                     /locus_tag="JN701_001864"
     CDS             13392..13802
                     /gene="fliS"
                     /locus_tag="JN701_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY7364329.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            13802..14168
                     /gene="fliT"
                     /locus_tag="JN701_001865"
                     /pseudo
     CDS             13802..14168
                     /gene="fliT"
                     /locus_tag="JN701_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            14246..15733
                     /gene="amyA"
                     /locus_tag="JN701_001866"
     CDS             14246..15733
                     /gene="amyA"
                     /locus_tag="JN701_001866"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7364330.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(15767..16180)
                     /locus_tag="JN701_001867"
     CDS             complement(15767..16180)
                     /locus_tag="JN701_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7364331.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            16367..17572
                     /gene="yedE"
                     /locus_tag="JN701_001868"
     CDS             16367..17572
                     /gene="yedE"
                     /locus_tag="JN701_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY7364332.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            17569..17802
                     /gene="yedF"
                     /locus_tag="JN701_001869"
     CDS             17569..17802
                     /gene="yedF"
                     /locus_tag="JN701_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY7364333.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            17909..18580
                     /locus_tag="JN701_001870"
     CDS             17909..18580
                     /locus_tag="JN701_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAY7364334.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(18620..>18979)
                     /locus_tag="JN701_001871"
     CDS             complement(18620..>18979)
                     /locus_tag="JN701_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7364335.1"
                     /translation="QPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVFAVPP
                     ACTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQ
                     SGRPLKQEPTEMIQATA"
BASE COUNT         4686 a         4921 c         4638 g         4734 t
ORIGIN      
        1 ttcattaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca gtgccgccgt
       61 tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt caccactttt
      121 gattgtttgc tgaaaattac gccgaaacga tcactgatca agcaaatctg cacgcgcagc
      181 gctcaaatat tgcaacattg accacagagt cagcaccgca gccacaaaga aaagtgcaat
      241 accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggccaacg ccaccatctg
      301 ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt tacccaactc
      361 cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg ttgccgccgg
      421 tagggtcacc caccagctgt gataatgctc ggttaccagc accatggcga tagccacgag
      481 aactttatct gctacagggt caaggaaagc accaaaccgg gtactctggt tccagcggcg
      541 tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc
      601 aaacggcgac caggtgacag gcagataaaa gaccaataca aagaatggga taaggatgac
      661 acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta actatctgtt
      721 gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga ccagaagatc
      781 ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac gctggcgtta
      841 cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccgacgttt tggcccgacg
      901 ccttcaatgg tttccaggga actggtattt ttgaccttcg cccgtttttt acggtgcccg
      961 ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga taacatgcag cgcgggtgaa
     1021 tctggcggca aactaaatcc ctcatcttcc ggctcaaaga acagcgtttc cagtccagcc
     1081 ttacgatctg ctcctttggc aacgccaagt agcagcggat gatttttatc ccatgagaca
     1141 tccagttcgg cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg
     1201 atcacatccg ggatcttact gtcgtcaatg gctttaccat aacgccgacg cagcacctga
     1261 ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa tgttatagcg ccgatactcc
     1321 gcacgcagcg ggccgttagc atcaaacacc acacaggaag cgacggtttg ttcgcccatg
     1381 gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa cacgctggca
     1441 agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa gtttgctggt taaggccgtc
     1501 gccgcattgg tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg tttggtttga
     1561 acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt tttatcgcta
     1621 agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag
     1681 aactgaccta cgaaggtttc taccacctcg ctcagttccg taccgccagg cactttcggg
     1741 aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg gacacaagcc
     1801 atgcccgcat cgaacgccac accaataacg tcaaggtcgt cgccggtatt ggaaacaaac
     1861 tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc cgcttcttca
     1921 aactccagat tctgactggc agtttccata cggctaatga gttgcgtaag cacctgatca
     1981 tctttgccag acaaaaacag gcgcacatac tcgacctgct gagcgtattc ttcttcactc
     2041 accagtcctt caacgcacgg tcccagacag cgccctatct gatattgcag acacggacgc
     2101 gagcgattgc gataaacact attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc
     2161 gccagagttt cacgtacggc atagccattc gggaacgggc cgaaatattc acctttggca
     2221 tgcttcgcac cacgatgcat cgccagacgc gggtgggtat cgccactcag gaagataaaa
     2281 ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag
     2341 ttgtgttcca gcaacagcgc ttcggtttct gtatgagtaa ccgttacatc aatttgctga
     2401 atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa atagctggaa
     2461 agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc agcatcgtac
     2521 atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc gtcaaactga
     2581 tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt gccaggtgag
     2641 tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg tagctgttca
     2701 ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg cccttggtga
     2761 tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt tctgtttttt
     2821 ctggttcgat ctggcttaac gccatttgtt gagcgatgtc agaagcaatg taacgctgcc
     2881 ctgaatagac agaacgaatc gcactcacga cttcctgcgg agccgcgcct ttgctgaggt
     2941 agcccgcagc accggcctgc atgactttcg ctggtaaagg gttttctgta tgaacggtaa
     3001 gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc
     3061 caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgtc cggcaccact
     3121 taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt atatcttcca
     3181 gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga acgttgatca
     3241 aagaaatatc tccagaaata gggataacgc tactgatagt tagtcattcg tatattagcg
     3301 gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc tctcgttttt
     3361 gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttac cggatcgttt
     3421 cacagtaagc cctacgatga aatgtgacaa aaattgactt tattcagcaa aaatgaaaat
     3481 cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa cattcatgat
     3541 aaaaatattt attggtcatt atattaacgt attttatagc actgccgata tcacgctcaa
     3601 aaaacaacca ctgctatttt tagcaaagct tatggtatac tccgccgcct taacattttt
     3661 caccgcaaat tttcattgca acatgacgag gaaaataaat gagtacgcct gatttttcta
     3721 ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc
     3781 tgatgctgca ggcgatggga caagctgacg cgggccgcgt gatgttaaag atggaaaaac
     3841 agcttgcgct gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa
     3901 ttaaacaagc ctaccgtcag taatataaaa ccggctgaga gcgtgccttt cagccggttt
     3961 ttgcatctgg cacgcagaaa gaaaagcgat caaattaagc cagtagcggc cgcgtaacag
     4021 gccacctggg tcttatttgg tgcattgatt tttttctgca tgtttttctg atggaaattg
     4081 accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc
     4141 gtccatttca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt
     4201 tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat
     4261 tgcagttcat cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag
     4321 cccagcgccc gatttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg
     4381 tgcgcggctt cccataatgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg
     4441 ccctgactaa agttttcagg gttcagcaca ggatcaattg cgagaaaatt ttttgcctga
     4501 taataactaa cccacgactc agggtaattg gtgtagaaag ccactttagg tcgcgtgaat
     4561 ggtaccgggt ggcgaacaca taacgaatag taatcgtact ccagctgctg agcctgaagc
     4621 tctatttcat ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg
     4681 cgacgccaac tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag
     4741 tatcatttat aatgataatt caaactatca cataaataag atttatatat aatttatatt
     4801 attcaggcaa tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat
     4861 gctgatttgt taagcagttt tatcacgctt gaaatggcgt cccgccccga caggtgaatc
     4921 gtcggggctg attttttctt attattgcag cagaaacttc tcgaggaact gtcgggtgcg
     4981 aggctgctgg ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgccccag
     5041 gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat
     5101 caccatcgtg cgcttttcct gcgccagctg gcggattgtg ttcaggactt cgcccaccag
     5161 ctctggatcc agcgctgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag
     5221 cgcacgcgca atcgctacac gctgctgttg accgccagac aaacgacgtg gatagctggt
     5281 ttctttaccg gccagcccaa ctttcgccag cagctcgcga gcgcgcgccg tggcctcttc
     5341 tttcggttcg cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg
     5401 cggaaacaaa ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag
     5461 cgatttttgc tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt
     5521 tcccgcttcc ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc
     5581 actcggaccg ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag
     5641 caccgtctga ccttggaatt ttttcaccag gttcttaact tcaatggcac tcatttgggt
     5701 tctctctcct ggcgattaag ttgattctca aaatggttct gcaacgtcga taacacagtc
     5761 gccatgatcc agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc
     5821 gaggtaatca actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac
     5881 gtatctttta ccagactgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc
     5941 tgtggcaaaa tcgcgcgacg catggtctgc cacggcgtca taccaatact ggcagcggct
     6001 tcccactgac ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc ggcataggcg
     6061 gcagtgttca gcgacagacc aatcattgct gacagaatgg gatctaattc gataccaaac
     6121 tgcggcaggc cgtaatagat cataaacagt tgtgcgatga gcggcgtacc acgaaaaata
     6181 gagatataaa aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg
     6241 agaataaacc cgagtagtaa gccaaaaaac atgccgccga tactgagttg cagggtatac
     6301 ccagcccctt tcaacaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg
     6361 agcgttcccg gagctaaacg tggggatgat aggcgaacag cgcaggcgcg ccgccggtat
     6421 gaataaacag aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagcc
     6481 ccgccatcgc ttttccggta tacacaggat caagcagaat gccttccaga cgcgccagca
     6541 atttcactgc ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat
     6601 cccagagtaa aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta
     6661 ggttaaccac tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt
     6721 cgctttcagg catcaggtgt tccagcccaa cagccaatcc ggcgtgagtt ccggcactgc
     6781 ccgatgcgac caccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca
     6841 gcgcactctc cacataaccc agcgcgccca gagcattaga accgccaacc ggaatgacat
     6901 acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat
     6961 cggtcagtgc gtcgcacatt tcaatctggg tattgaacag attcagcaac aaacgattgc
     7021 cgttggttaa atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt
     7081 gcaggccgag tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgcgc
     7141 cggcagtaat cagcgtatcg gcaccttcgc gcagggcatc tgccgcgaga aattccagct
     7201 tacgtaattt attgccgccc attgccatgg gtgtgacgtc atcccgtttg atgaaaattt
     7261 cccgtccaag ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa
     7321 actccagccg tggaaaacgg gttaaattat gcagtggcat aatagcctcc gatgtgtgtt
     7381 gttgtgattt tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag
     7441 cgcccttttt tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga
     7501 aagggcttgc agagtgccat ctttttgcat ttccgcaatt gcatcgttca ctgctttcag
     7561 caggtcttca tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc
     7621 ggttactgcc agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac
     7681 gaggatcgca tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata
     7741 ggtacgcaca tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag
     7801 accgacaccc acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc
     7861 ttttttcacc agcccctgaa taccagaaat ggtgtacggg gtagagaaat cgtatttttt
     7921 cttgcgctca tcagaaatgg tgacctgatt aatcaccaca tcaatacgtt tagagtccag
     7981 cgacgctagc ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt
     8041 tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa
     8101 actgaacggc ggataagttc cttccagccc taccagtagc gtaccgcgct ctttgacttt
     8161 attaagcaga ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgccacggc
     8221 catcacaccc atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt
     8281 tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca
     8341 tcttattctt atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc
     8401 ggtacacgtt tgacgctgat agtgttgata cttccgtaat gcgatgcggt aattgttggt
     8461 gctggtggtg ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc
     8521 gagggaaata ttttgctcat gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc
     8581 acgcaccgta cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat
     8641 atcgaaaaaa tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc
     8701 ccatgactgt tgttccactt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat
     8761 ctcgatttta ttcactattt tgccgtcgcg aactaaggta atgcgatcga gtaatttacg
     8821 gcaatgcgcg caatgggcct ggctgtgttt aaagtcttta agatagcggc ttaatggccg
     8881 tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat
     8941 ttactgacgt tataacttac ccagtttagt gcttaaccgt ttaatggcct ggctgtgtaa
     9001 ctgactgacc cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc
     9061 ttcctggtaa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatagcttc
     9121 catcacccgc tggcgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg
     9181 atcatcagta accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa
     9241 aagctggctg ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa
     9301 acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat
     9361 tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg
     9421 aagttcatcc agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc
     9481 ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag
     9541 atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac
     9601 cagcgggaca taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag
     9661 tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc
     9721 agggttttta atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa
     9781 attccatgca gaaaaaaccc cgccggtggc ggggaagcac gttgctgaca aattgcgctt
     9841 tatgttgccg gatgcggcgt aaacgcctta tccggcctac aaaaatgtgc aaattcaata
     9901 aattgcaatt caccttgtag gcctgataag tgcagcgcat caggcaattt ggcgttgccg
     9961 tcagtctcag ttaatcaggt tacgacgatt aaccctgcag cagagacaga acctgctgcg
    10021 gtacctggtt ggcttttgcc agcacggagt taccggcctg ctggatgatc tgcgctttcg
    10081 acatgttgga cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcagaca
    10141 ggttggtagt ggtgttgttc aggttggtga ctgcagaatc cagacggttc tgaatagcac
    10201 ccagggatga acggaatttg tcgatggagc tgatagcttc atccagggca gccagaggat
    10261 ctttggttgc agtgcctgca ctggtggttt cagtggtcag tttgcctgta gagttaacat
    10321 aagcctgtgc cccaacagta gctgcaaatt taccagttgc acctgcgcca tttgaagcta
    10381 cagttactgt accggtatcc ttatcgacgt tgtagttgat agtgtaagat gcggttgtgg
    10441 tgagctcttt gtctgcatca gtataggtgt cattcgaatt accagcacca ttagtattcg
    10501 caccaccgaa ggacgccgtt gcagactgaa tacctgtatt atacttaatt gtcgcaccag
    10561 taactgctgt gttagccgtt gcagcactct gcactgtgct cagtacagta tcagcgctta
    10621 cagttttggt gtaggttacg ccagaagttg ccgctgcacc aaagttataa gttgtgccag
    10681 acaactggat agaaccagtt gtcgctaagg tactaccgct agcaaacaga tcggacaatt
    10741 tcgctgaaga tgcaatgccg gcgttgtttg tagataggtt accagtggcg tccaggtacg
    10801 cggctttacc accgatggtc acatcgccat tagcatctac atcaaacttc acatcaccag
    10861 tactacgagt ataaacacct gatgcagtag tgccagccgc tggtttcaga gtattcgcaa
    10921 agttaacaac atcgccatct gcaactgttg cttgagtggt gaagttccct ttagccgcat
    10981 cataagtata gatcgcagca ctcgagccag tagtagtaac tgtatctccg gttttcaggc
    11041 gagacagtgc atcgctagcg ctgagtgctg tattgtttgt ggtcacagca taaggacctg
    11101 ttcccgttgc accagcagcg gtcagatcgc tgactgtagc agctttgttc gcaatagtgc
    11161 ctttgccatt cacgttaaaa ccattcagcc ccagcgtatc tgagtcaatt ttcttcagat
    11221 caatcgtgat agtctggccg tcattcgcac caacctgaat tttcattgaa ccgtctttcg
    11281 ccagtacgtt cacgccgttg aactgggtct ggccagatac gcggtcaatt tcgtccagac
    11341 gggatttgat ttcgtcctga atggagtcca gatccgaatc ggagttagtc ccggtagaag
    11401 cctgaaccgt cagttcaaga atacgctgta agttgttgtt gatttcggac agcgcgcctt
    11461 cagtggtctg cgcaacggag ataccgtcgt tggcgttacg ggccgcctga gtcaggcctt
    11521 taatattaga agtaaaacgg ttagcaatcg cctgacctgc ggcgtcatcc ttcgcgctgt
    11581 taatacgcaa gccagaagac agacgctcga tagaactcga cagcgcagac tggttcttgt
    11641 tgatattatt ttgagtgatc agcgagaggc tgttggtatt aatgacttgt gccatgattc
    11701 gttatcctat attgcaagtc gttgattacg tattgggttt ccacccgtcg gctcaatcgc
    11761 cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga atttttttca caaacagcca
    11821 ttttttgtta gtcgccgaaa tactcttttc tctgcccctt attcccgcta ttaaaaaaaa
    11881 caattaaacg taaactttgc gcaattcagg ccaataaccc cggtattcgt tttacgtgtc
    11941 gaaagataaa aggaaatcgc atggcaagta tttcatcgct gggagtcggg tcaggtctgg
    12001 atttaagttc aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta acccctattt
    12061 caaatcagca atcgtcgttt accgctaaac ttagcgccta cggtacgctg aaaagcgcgc
    12121 tgacgacttt ccagaccgcc aatactgcat tgtctaaagc cgatcttttt tccgccacca
    12181 gcactaccag cagcaccacc gcgttcagtg ccaccactgc gggtaacgcc atcgccggga
    12241 aatacaccat cagcgtcacc catctggcgc aggcgcaaac cctgaccacg cgcaccacca
    12301 gagatgatac gaaaacggcg atcgccacca gcgacagcaa actcaccatt caacaaggcg
    12361 gcgacaaaga tccgattacc attgatatca gcgcggctaa ctcatcgtta agcgggatcc
    12421 gtgatgctat caacaacgca aaagcgggcg taagtgcgag catcattaac gtgggtaacg
    12481 gtgaatatcg tctgtcagtc acatcaaatg acaccggcct tgataatgcg atgacactct
    12541 cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt gccagcagca
    12601 acggtatgga ggtctcggtc gccgccaaga atgcgcagct gacggtcaac aacgtcgcca
    12661 ttgagaacag cagcaacacc atcagcgacg cgctggaaaa catcaccctg aacctgaacg
    12721 atgtcaccac gggcaaccag acgctaacca tcactcagga cacctccaaa gcgcaaacgg
    12781 cgattaaaga ctgggtgaat gcctataact cgttaataga taccttcagt agcctgacca
    12841 aatacaccgc cgtagatgcg ggagctgata gccagagttc tagcaatggc gcactgctcg
    12901 gcgactccac gctgcggacg attcagacgc agttgaagtc gatgctgagt aataccgtca
    12961 gttcttccag ctataaaacg ctggcgcaga ttggtatcac gaccgatccc agcgatggca
    13021 aactggaact ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag
    13081 gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac gaccaccatc ggcagcaacc
    13141 tgaccagttg gctttcgaca acgggcatta ttaaagccgc taccgatggc gttagtaaga
    13201 ccctgaataa gttaactaaa gactacaacg ccgccagcga tcgcattgat gcgcaggtcg
    13261 cgcgctacaa aaaacaattt acccaactgg acgttttaat gacctcgtta aacagcacca
    13321 gcagctactt aacgcagcag ttcgaaaaca acagtaattc caagtaatca ttattcatcg
    13381 ggagacaggt catgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa
    13441 gcgccgtaat gagcgccagc cagcagcagc tggtcaccat gctatttgat ggagtgctga
    13501 gcgcactggt tagagcgcgc ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct
    13561 ctttgtcaaa agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga
    13621 gcaaagacga actaacccaa aacttgattg ctctttatag ctatatggtc aggcgcttgc
    13681 tgcaagccaa tttacgcaac gatgtctccg cagtcgaaga agtggaagca ttaatgcgca
    13741 atattgccga tgcctggaaa gagtcgttac tctccccttc tttgattcag gacccagtct
    13801 gatgaaccat gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct
    13861 catgttacgc ctggcaacgg aagaacaatg ggacgaactc atcgccagcg aaatggcgta
    13921 tgtgaatgcg gtgcaggagt atagcacacc tgactgaaga ggttgccccg tccaccacga
    13981 tgcaggagca gctccgcccg atgctgcgcc tgattctcga caacgaaagc aaggtaaagc
    14041 agttattaca gattcgtatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa
    14101 aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg caagataact
    14161 tctcgtgaat cttaacgagc taatggttcg cgaataatcc gattacggct acgcttctaa
    14221 tgttcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg
    14281 gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgccg acggttttaa
    14341 tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcgggta
    14401 ttcggtcggc tacgactcct ataatttatt tgatttaggc gagtttgatc agaaaggcag
    14461 catccctact aaatatggcg ataaagtaca attgctggcc gccattgacg ccctgaaacg
    14521 taatgacatt gcggtgctgt tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa
    14581 agaagctatt cgcgtgcagc gtgtaaatgc tgacgaccgt acgcaaattg atgaagaaat
    14641 cattgagtgc gaaggctgga cgcgttacac cttccccgcc cgtgccgggc aatactcaca
    14701 gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga
    14761 tggcatattt aaaattgtta acgactacac cggcgaaggc tggaacgatc aggttgatga
    14821 tgaattaggt aatttcgatt atctgatggg cgagaatatc gattttcgca atcatgccgt
    14881 gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggttt
    14941 tcgtcttgat gcggtcaaac atattccggc gtggttttat aaagagtgga tcgaacacgt
    15001 acaggaagtt gcgccaaagc cgctgtttat tgtggcggag tactggtcgc atgaagtcga
    15061 taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgttcg atgcgccgct
    15121 gcagatgaaa ttccatgaag catcgcgcat ggggcgcgac tacgacatga cgcagatttt
    15181 cacgggtaca ttagtggaag ccgatccttt ccacgccgtg acgctcgttg ccaatcacga
    15241 cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg tggtttaaac cgctggcgta
    15301 tgccttaatt ttgttgcggg aaaatggcgt tccttcggta ttctatccgg acctctacgg
    15361 tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata tgccaataat
    15421 cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt
    15481 atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg aatatcccgg
    15541 ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa accattcatc tgggagagaa
    15601 ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg taataaccga
    15661 cgaacacggc gaagcaacct tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga
    15721 agaggtgatt taaattcatc cccggcggca agccggggag atttcattac ggcagtggcg
    15781 tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct
    15841 ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac
    15901 gttttacggt aacgttagtg agatcatcag acatcaatgc caacttaccc ggcacagcaa
    15961 tcacccgctg ccactgacgg caatccagag tatcaccctc tttagtgaca atcaggctgc
    16021 ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag tagcccgcca
    16081 gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg
    16141 ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct gcatgagaag
    16201 aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggtt cagagagaaa
    16261 tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc atgatgtatg
    16321 attcgcgact cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca
    16381 attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca tcgcggcggg
    16441 tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca cgggtgaatt
    16501 tacccgttgg ggcggccagc tcctgcagct gtttggcgtc catgctgaag agtggggtta
    16561 ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggga tgatgatcct
    16621 cggcatgttt ggcggctgct ttgccgcagc gctgtgggcc aacaatgtca aactgcgcat
    16681 gccacgcagc cgtatccgca ttatgcaggc cattattggc ggtattatcg ctggttttgg
    16741 cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggtattc ctcagttctc
    16801 gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcacgctt
    16861 taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc
    16921 gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt
    16981 cggtatgctg ggctgggcgc tgctcacggc gatgaaccaa ccaaaactgg ggctggcaat
    17041 gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct ttacttcggc
    17101 gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca ttatcggtat
    17161 ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat
    17221 gtgggcggga ccaaacgcgg taattggtgg tttactgttt ggttttggca tcgtgctggc
    17281 aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg
    17341 ggtcggtctg ggcaatgtga tcggctcgac gattctggcg tattactggg atgatttcgc
    17401 tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacctttg gcccgatggg
    17461 tggcctgctg gtgacatatt tgctgttgtt tactgcgctg atgttgatta tcggctggga
    17521 aaaacgcttc ttccgccgtg cggcaccgca gactgctaag gagatcgcat gaaaaatatc
    17581 gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc agtcgcaacc
    17641 cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg aagtggtgag cgactgtcca
    17701 cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt gcgggatatt
    17761 cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg
    17821 gcccacagcg ggaacatcgt tgcctctgac acatagattt tacccgcccc ctctcctatt
    17881 cttttcagta tgatctcagg aggtttctat gtgtggacgc tttgcccaat cccaaacgcg
    17941 tgaagattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct acgatcccga
    18001 acccattggc agatacaacg tcgcgccggg aaccaaagtt cttctgctca gtgaacgtga
    18061 tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt gggataaacc
    18121 gccgctgatt aacgcccgcg tagaaactgc cgcaaccagt cgcatgttta aaccgctctg
    18181 gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa aagaaggcga
    18241 caaaaaacag ccttttttta tctatcgtgc tgatggacaa cctattttta tggccgcgat
    18301 aggtagcaca ccatttgagc atggtgacga agccgaagga tttttaattg tcactgctgc
    18361 agcagaccag ggtctggtag acattcatga ccgccgcccg ctggttctgt cgccagaagc
    18421 tgcacaggaa tggatgcggc aagagattgg cggtaaagaa gcatcagaaa ttgctaccaa
    18481 cggctgtgtg ccagcaaacc agttcacctg gcacccggta tcgcgcgcgg taggtaatgt
    18541 caaaaaccag ggcgcggagt taattcaacc tgtttgttga catcctccac gccctgaagg
    18601 acgaggctcc ctgctacgtt caggctgtcg cctgaatcat ttcggtgggt tcctgcttca
    18661 acgggcggcc tgactgcacc atctctccac aggcaagaac ggcgtgcccc gccgctaaaa
    18721 tgttacgagc gccatttaca tcggcgttcg ctgtatatcc acatacctgg catctgaatt
    18781 tactttgtga caggcggttt tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg
    18841 tgcacgctgg cggaacagca aacacctgac cgccacgcca gagctgcttg tactcaagct
    18901 ggcggcgcat ttcataccag ccctgatcca gtatcgaacg gtttaaacct gattttgccc
    18961 ggacattgcg ccccggctg
//
LOCUS       DADLTD010000068        18909 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLTD010000068 DADLTD010000000
VERSION     DADLTD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.068"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JN701_001872"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JN701_001872"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY7364336.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JN701_001873"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JN701_001873"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY7364337.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JN701_001874"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JN701_001874"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY7364338.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JN701_001875"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JN701_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY7364339.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JN701_001876"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JN701_001876"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY7364340.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JN701_001877"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JN701_001877"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7364341.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="JN701_001878"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="JN701_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY7364342.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JN701_001879"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JN701_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7364343.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     DMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JN701_001880"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JN701_001880"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY7364344.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JN701_001881"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JN701_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY7364345.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /locus_tag="JN701_001882"
     CDS             complement(14846..15541)
                     /locus_tag="JN701_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7364346.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="JN701_001883"
     CDS             complement(15664..16701)
                     /locus_tag="JN701_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7364347.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17033..17521
                     /gene="yhhY"
                     /locus_tag="JN701_001884"
     CDS             17033..17521
                     /gene="yhhY"
                     /locus_tag="JN701_001884"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7364348.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17758..>18909
                     /gene="hcp"
                     /locus_tag="JN701_001885"
     CDS             17758..>18909
                     /gene="hcp"
                     /locus_tag="JN701_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAY7364349.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPM"
BASE COUNT         4826 a         5012 c         4602 g         4469 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gctttcagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
     5041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaattc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccacct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacaccg ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcaca cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaattat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gaacccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 ggagtagttc agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgtcggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa
    12361 attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg
    12481 caccagaaat gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag
    13261 catagcaaac gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gcaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatacgac accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat
    14101 ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt
    15061 ccatatcctg atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctaggat
    15601 gatagagggt gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcacg ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg
    16201 cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc
    16321 aaagcgacga ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag
    16381 ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag
    16501 cttaacatcg ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca
    16861 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
    16921 ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca
    16981 tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga
    17041 gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca
    17101 gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga
    17161 gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt
    17221 gggccatctc accattgacg tgcaacagcg cccacgccgc agtcatgttg ccgattttgg
    17281 tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat
    17341 gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga
    17401 taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa
    17461 gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta
    17521 agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct
    17581 ttaaggtatt tatttaccca atacatatat tgagatttaa ctcataatta cttcctaaag
    17641 tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat
    17701 taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg
    17761 agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt
    17821 gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca
    17881 ttctcactct taaataatat taataatacg gggcttggtt cacagttcca tggtataaca
    17941 ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa caataatgaa
    18001 caattattta tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat
    18061 tattatatac aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac
    18121 caactggata cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc
    18181 gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgatt gtttttacca
    18241 aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa acagcaaagc tattggcagg
    18301 ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa
    18361 aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc
    18421 atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca
    18481 gaaataaata gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac
    18541 ataatagaaa taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag
    18601 tttaataaaa ctgtaagaaa aaattttctt atcaaacttg caaaaacctc cgaagcatct
    18661 ggaagattca acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat
    18721 aatgttcacc ataaactatc tctagatgat ggtggtacta atgatttcga aaatttagta
    18781 ttaatcgaaa acgaaccata tcataaagtt tttactaaca tgcaatcacg aatagctaag
    18841 ggaatattag taggtgaaag caaaatcact ccctgggcca ttccatctgg ctcaatttat
    18901 cctcccatg
//
LOCUS       DADLTD010000069        18853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLTD010000069 DADLTD010000000
VERSION     DADLTD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.069"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(353..1300)
                     /locus_tag="JN701_001886"
     CDS             complement(353..1300)
                     /locus_tag="JN701_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364350.1"
                     /translation="MPRYKKGIRNNCFHQNYTHDVLFPGATFRTRHNGECAILGRSDD
                     KSRRGYYVVEFKDSGIIKEAYGSHIKTGSVSDEAFPSSEEERRKLLMTPKYYGVGYIG
                     NGCHSTIENTRTHQRTRAFILWHNMLARCYMTTKGKLYFKGYKGVTVCERWHNFQNFC
                     NDLPKLHGYNKWKDNPGEYELDKDYSHRRIYSADTVAFISTEENAKEAGLRRVAMKIP
                     SGHYHEINKIRDEILMEAEDELKNNQINYEVVLDGNMKVILCETPYGTVLFWPLTKKI
                     QRNCYMIDGDVQVYVYYLRWLILQWENRNPDINCIATTC"
     gene            complement(2149..2811)
                     /locus_tag="JN701_001887"
     CDS             complement(2149..2811)
                     /locus_tag="JN701_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364351.1"
                     /translation="MKKLITLPLLISMTGLLFANQSLAITQSQMQMRATATCDSYASM
                     SRIASPGVVYEVCLNGAKESMQGRNSACESKIRQFNKQADTLHGMERAEYIEISQAYR
                     MGCNVGKQTPKINSENKQAIAKPLSQKEIEERQEIQNYSMEIRNAFSQIRSGAPDLTG
                     QKCSIKVWFSQSGNVLSIKVESGDNDACSYVLKRTQKIKLSAMNDSQYKVFRNTPIDF
                     SF"
     gene            complement(2808..3212)
                     /locus_tag="JN701_001888"
     CDS             complement(2808..3212)
                     /locus_tag="JN701_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364352.1"
                     /translation="MKRLIPILASAASMTIPVAAMADNIVCHGGWSIERHVTGSASSG
                     FMSYMKDVGVIKLLHNGKLISQKRIQDIQQSVYQTSGAPLVNYEFDVDKNSFYILALD
                     MSTQGASGGEPDIKLWGTDVMGIDCILQVGAE"
     gene            3646..3825
                     /locus_tag="JN701_001889"
                     /pseudo
     CDS             3646..3825
                     /locus_tag="JN701_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimH domain protein"
     gene            4159..6438
                     /gene="ydeP"
                     /locus_tag="JN701_001890"
     CDS             4159..6438
                     /gene="ydeP"
                     /locus_tag="JN701_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY7364353.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            6686..6883
                     /gene="safA"
                     /locus_tag="JN701_001891"
     CDS             6686..6883
                     /gene="safA"
                     /locus_tag="JN701_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY7364354.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            6958..7719
                     /gene="ydeO"
                     /locus_tag="JN701_001892"
     CDS             6958..7719
                     /gene="ydeO"
                     /locus_tag="JN701_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY7364355.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            8121..9803
                     /locus_tag="JN701_001893"
     CDS             8121..9803
                     /locus_tag="JN701_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7364356.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAIDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            9855..11012
                     /locus_tag="JN701_001894"
     CDS             9855..11012
                     /locus_tag="JN701_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7364357.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(10978..11100)
                     /locus_tag="JN701_001895"
     CDS             complement(10978..11100)
                     /locus_tag="JN701_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364358.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            11315..13000
                     /locus_tag="JN701_001896"
     CDS             11315..13000
                     /locus_tag="JN701_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY7364359.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            13038..15410
                     /locus_tag="JN701_001897"
     CDS             13038..15410
                     /locus_tag="JN701_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7364360.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            15455..18250
                     /locus_tag="JN701_001898"
     CDS             15455..18250
                     /locus_tag="JN701_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7364361.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIISDK
                     ENRVNGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIV
                     QFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            18612..>18853
                     /locus_tag="JN701_001899"
     CDS             18612..>18853
                     /locus_tag="JN701_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7364362.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         5425 a         4081 c         4072 g         5275 t
ORIGIN      
        1 gtggtgacgg cagttgaaac caatgtgttg aggttgcatg gacggttaag ccgagaatac
       61 ggtagtaagt gagatatggg gtttggtcgt tgttaatgag caaccatgac cgccaacgac
      121 tggattctcc cttggtttat gttggtgcgc ataatgtgtt gaacatgtta aatgactgca
      181 cgaagtgctg gcatttagcc ttctgcaatg aactgccaga cgttgtcact tgttttctgg
      241 catagaaaat cactgcaaca gcggaattac agatacatcc tgaatgttcg tttttcttat
      301 taatagatag cattgtgaaa tgccggttat tttatgacta tttatagcat catcaacagg
      361 ttgtagcaat acagtttatg tcgggatttc tgttttccca ctgaagtatg agccagcgca
      421 ggtaataaac ataaacctgt acatcaccat caatcatgta gcaattacgc tgaatctttt
      481 ttgtgagagg ccagaacaac actgtgccat aaggagtttc acacaggatt actttcatat
      541 tcccgtcaag aacaacttca taattaattt gattgttttt taattcatct tcagcctcca
      601 ttaatatctc atctcgaatt ttatttattt catgataatg cccggaagga atcttcatgg
      661 caacgcgtcg taatccagct tccttggcat tttcttctgt gctgataaat gccaccgtgt
      721 cagcagaata aatccggcga tgtgagtaat ccttatcgag ctcatactct cccggattat
      781 ccttccattt gttgtagcca tgcaattttg gaaggtcatt gcagaagttc tgaaagttat
      841 gccatcgctc gcatacagtg acacctttgt agcctttaaa atacagcttc cctttggttg
      901 tcatgtagca ccttgccagc atgttatgcc agagtatgaa tgctctcgtt ctttggtgtg
      961 ttcgggtgtt ttcaatagtt gaatgacatc cattaccaat atatccgacg ccgtaatatt
     1021 ttggtgtcat cagtaacttt cgtcgttctt cctccgatga tggaaaagct tcgtctgata
     1081 cagaaccagt tttaatatgc gagccatagg cttccttgat gatgccactg tctttgaatt
     1141 ccacgacata atagcctcgc cttgatttat catcagagcg gccaagaatg gcgcattctc
     1201 cattatgtcg ggttctgaat gttgcgccgg ggaacaatac gtcatgagta taattttgat
     1261 gaaagcaatt attacggata ccttttttgt agcggggcat gatatttctc ctgcgtgatt
     1321 taactgaaag ggaagggagt aaaaagaatc ggggcgggtc cttctttttc gattcacctt
     1381 cagtcaggca ctcatgccgg agagatggat tatatttatg taatcacttc aaaaaataaa
     1441 atataaaatt tttcaaacaa tgaattgtga cagatgatgg tacaaaccgt caggcgctct
     1501 gtgtggcatc attaatacgg cacatgcaaa tgcgtcaggt tgcagccgga tgcgctcaga
     1561 tggctgtaca gatgcaataa acagggcgca acaggggggg ggttgccagt gtgagagggc
     1621 gcatatgcct gacaattgca ggactgttta tttatacagt atttggtatt ttgcttacat
     1681 cgtgaccgct gactgcctgt atgcggtttt ggggtgattt tggccttaga catggctttg
     1741 cattgctact gactctggcg ttgcgcatgg tgcattacag ggagcgcact cagggtcagg
     1801 actgcgccgg aatcacccga tagggtgggt aaataaaaaa ataaatataa tcgttttaca
     1861 gatttaaata tctgtgctac catcgccaaa tcgccaaccg gggttaattc ttttatatgt
     1921 gttctgttat tctcttatat agacttgctg tttaattgtt attatcggtt ttctgtactt
     1981 atctgcatct gtctttgatg tttcaatatt atcccaatat taagccctcc gcaggagagc
     2041 catgcggctt gagctgacaa taataagaaa agaaaaataa gaatctccac aaaagagaag
     2101 actcctatca gtaataaaaa tcagaaactt atcttatgag tagtgttttt aaaaactaaa
     2161 atcaataggt gtatttctaa acactttgta ctggctatca ttcattgcac tgagcttgat
     2221 tttttgggtt ctctttaaaa cataagagca tgcatcatta tcaccggact ctacttttat
     2281 tgacaacaca ttaccacttt gagagaacca tactttaata gagcactttt gtcctgttaa
     2341 gtcaggtgcg ccactcctga tttggctaaa tgcattacga atttccatgc tataattttg
     2401 tatttcttgc ctttcctcta tttccttttg tgataagggt ttcgctattg cttgcttgtt
     2461 ttcactattt atcttgggtg tttgctttcc aacattgcaa cccatgcgat atgcctgaga
     2521 aatttcaata tactcagcgc gttccattcc atgcaatgta tccgcctgtt tattgaattg
     2581 tcgtattttg ctttcgcatg ctgagtttcg accctgcatc gattctttag ctccattcag
     2641 acacacttca taaaccacgc ctggactggc aattctggac atacttgcgt aagaatcaca
     2701 tgtagcagtc gctctcatct gcatctgtga ttgcgtaatc gccagtgact gatttgcaaa
     2761 taacaaaccg gtcattgata tcaataaggg aagggtgatt aattttttca ttcagcacct
     2821 acctgtaaga tgcaatcaat tcccattacg tcagtacccc aaagtttgat atccggctcg
     2881 ccacctgatg caccctgagt gctcatatcc agagcaagga tatagaaact gtttttatca
     2941 acatcaaact cgtagttaac aagtggagca ccactggtct gatacaccga ttgctgaatg
     3001 tcctgaatgc gtttttgtga aatgagcttg ccgttatgca ataactttat gacgccaaca
     3061 tctttcatgt agctcatgaa tccactactg gcactaccgg taacgtgtcg ctcaatgctc
     3121 cagccgccat gacaaacaat attgtctgcc attgcagcaa ccggaatggt catgcttgcg
     3181 gcagaggcaa ggattggaat caatcgtttc atttgaatca gttcctgaag cgaacattca
     3241 gaacactcaa caagcccagt gaaggccgca agacatttca accgttaggc gaaccaacca
     3301 gactcagcac atccatattc tgcgagaaca aggatgccca caaatcactg gattctccct
     3361 tggtttatgt tggtgcgcat aatgtatatt atgttaaatc atatctgagt gatgcgaagt
     3421 gcttttctgg tagtcgttat tcgttcaggg ttccagaact taagcctctc ctctttcata
     3481 attggcctgt gcatgacgga tataggctgg ctggtatgca gatttgccgt ttgccgcatc
     3541 ttcccagcgt tgaagactca acgcgtgcaa atgagcacgc cctctttcca tcaacacttc
     3601 aggcagcttg cgggaatgag tccactacgg tatcagaaat gggaagtggg tgtctctctg
     3661 atgcgtaatg gaaaaatact ggctacggga gaaaatgtct ccctggggac agtaaataaa
     3721 agcaaagtat ctctgggttt aagcgcaacc tatggacaaa caggaaacaa ggttgcagcc
     3781 ggaaccgtcc agtcagtcat tggtgtcact tttatctatg aataattgtg attttgatct
     3841 tagtctatgc gctccattca cgaaaattgc gcggataatt attttgtgaa ggctattatc
     3901 ctacacctgt aagaaaatcc gcgcacaatt gaagccagag tcttattttt tatattgaaa
     3961 aatatgttaa tttaaacact atgagagatg atgcttaact tatcgtattt aatctattgt
     4021 ttaacgataa tagattaaaa atagaaagag agtagtcccc tactcttata tatccatgtt
     4081 ggcgataatc cctttgtatg tactgtgcat catcgctatt acaaatccta ataattcatt
     4141 tccacacagg ataagtagat gaagaaaaaa attgaatcct accagggtgc tgcaggtggt
     4201 tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc agatggatat acgccaggat
     4261 gttattgcca tgtttgacat gaataagcca gagggctttg actgtccggg ttgtgcatgg
     4321 ccagatccta agcacactgc gtcattcgac atttgtgaaa acggcgcaaa agcaatcgcc
     4381 tgggaagtca cggataagca ggtaaatgcc tctttctttg ctgagaatac ggttcaatca
     4441 ttacttacct ggggagatca cgagcttgag gctgcgggac gtctcactca gcctttgaaa
     4501 tatgatgccg tcagcgactg ttacaagcca ttaagctggc aacaagcttt cgacgaaatt
     4561 ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg aattctatac ttcgggccgc
     4621 acttccaatg aagctgcctt tctttatcag ctttttgccc gtgaatacgg gagcaataac
     4681 tttcccgact gctccaacat gtgccatgaa ccgaccagtg tgggtttggc agcgagtatc
     4741 ggcgtgggta aagggactgt gttgctggaa gactttgaga agtgcgattt agtcatttgc
     4801 attgggcata accctggtac aaaccacccc cgcatgctga cttcgttgcg cgctttagtg
     4861 aaacggggag cgaaaatgat cgccatcaat cctttacagg aacgtggcct ggagcgattt
     4921 accgcaccgc aaaacccgtt tgaaatgctg acgaactctg agactcagtt ggccagtgcg
     4981 tactacaacg tgcgcattgg tggcgatatg gcgttgctca aggggatgat gcgcctgtta
     5041 attgagcgcg atgatgctgc aagtgccgca ggtcggccct cattgcttga tgacgaattt
     5101 attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg acgttctcaa ttccgagtgg
     5161 aaagatatcg aacatatttc tggactaagt cagacacaaa tcgccgaact ggctgacgca
     5221 tatgccgctg ccgatcgcac cattatctgt tacggaatgg ggatcactca gcacgaacat
     5281 ggtacccaga atgtacagca actggtcaat ctgctgttga tgaaaggtaa cattggcaag
     5341 cctggtgcgg gtatctgccc actacgaggt cactctaatg tacagggcga ccgaaccgtc
     5401 ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc tgggtgagcg ctatggcttc
     5461 accccacctc atgcacctgg acatgctgca attgccagca tgcaagcaat atgcacgggg
     5521 caggctcgag cattgatctg catggggggc aactttgcac tggcaatgcc agatcgggaa
     5581 gcgagcgctg taccgttaac gcaattagat ttggcggtac acgtagccac taagcttaac
     5641 cgctctcatc tgctgaccgc acggcatagc tatattctgc cggttctggg gcgtagcgag
     5701 attgacatgc aaaaaagcgg tgcgcaggcg gtaaccgttg aggattcaat gtcgatgatt
     5761 catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc tgaaatcaga gtgtgcagtg
     5821 gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg tagcctggga gtatctggtg
     5881 gaagattatg atcgcattcg caatgacatt gaagctgtgt taccagagtt cgccgactat
     5941 aaccagcgca tccgacatcc tggaggtttt cacctgataa atgcagctgc tgaaaggcgc
     6001 tggatgacgc cgtcaggtaa ggctaatttc attaccagca aagggctgtt agaagatccc
     6061 tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac gcagccacga tcagtacaac
     6121 acgacgattt atggtatgga tgatcgctat cgaggggtat tcggtcaacg agatgtggtc
     6181 tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa acggcgaaag agttaatctt
     6241 attgcgctta cgccagacgg taagcgcagc tcacgccgca tggatagatt aaaagtggtc
     6301 atttacccta tggctgaccg ctcactggtg acctattttc cagaatcgaa tcacatgcta
     6361 actcttgata accacgatcc attaagtggc attcctggtt ataaaagtat tccggttgaa
     6421 ttagaaccat caaattaatt cgcctcctca tttcttctgc tgtcatccgc acagcagaag
     6481 aatttcttat tgactattat cgcgcaattt gctcacattg atcaaattaa accacacact
     6541 ataagatata aatctctgcc tacagctgta agaaactccg ctcagtactg aagtgccagt
     6601 cctatttcct cttttctcca gcctgttata ttaagcatac tgattaacaa tttttaacgt
     6661 tatccgctaa ataaacatat ttgaaatgca tgcgaccaca gtgaaaaaca aaatcacgca
     6721 aagagacaac tataaagaaa tcatgtctgt aattgtggtt gtcttattac tgacccttac
     6781 gttgatagcc attttttcgg caattgatca gctgggtatt tcagaaatgg gtcgcattgc
     6841 aagagatctt acacatttca ttatcaatag tttgcaagac tgaaaataaa cagcaaatta
     6901 taaatatgaa atgttaaaaa agtatcgata aaaactttat tgttttaagg agataaaatg
     6961 tcgctcgttt gttctgttat atttattcat catgccttca acgctaacat tttagataaa
     7021 gattacgcct tctctgacgg cgagatcctg atggtagata acgctgttcg tacgcatttt
     7081 gaaccttatg agcggcattt taaagagatc ggatttactg aaaataccat taaaaaatat
     7141 ctacaatgca ctaacatcca gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct
     7201 tcaaatgtac cgactggatt gcttaatgaa atgattgctt atctcaactc ggaagaacgc
     7261 aatcatcata atttttcaga acttttgctt ttttcttgcc tgtctatttt tgccgcatgc
     7321 aaaggtttca ttacactatt aactaacggt gtgctatccg tttccggaaa agtgagaaat
     7381 attgtcaaca tgaagctggc gcacccatgg aagctgaaag atatttgtga ctgcctgtac
     7441 atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc aaacgacatt ctcacagatt
     7501 ctattagatg ccagaatgca gcacgccaaa aatttgatac gcgtagaagg ttcagtcaat
     7561 aaaattgccg aacaatgtgg ttatgccagt acttcttatt ttatttatgc gttccgcaaa
     7621 catttcggca acagtccgaa gagagtttct aaggagtacc gttgtcaaag tcacacgggt
     7681 atgaatacgg gcaacacgat gagtgcttta gctatttgat tatttgctaa cgagtagtca
     7741 accacgcacg ctacgtaaga attatatggg gcagccattc cctgccccgc gttgttttta
     7801 ggcgatatat ttattgaaat aaataagtga catccatcac atatttatgc acttgcataa
     7861 cctgttgcat gattatttat gttctcaatt cagcattttg tcagtaaaat gcaataattt
     7921 attaaatatc aataaattag ttgtttatcg gcgagaaatt acttaataga acagaaagta
     7981 atgtcaacgc tttatggact gttatttccc tttttttagc taaatctgct atctctttat
     8041 gtgactaact tcacttacat ccacttattt ctcttcgtaa aattacttcg gaattaagta
     8101 caataagaag aggaacattt atgaagtctg cattaaagaa aagtgtcgta agtacctcga
     8161 tatctttgat actggcatct ggtatggctg catttgctgc tcatgcggca gatgatgtaa
     8221 agctgaaagc aaccaaaaca aacgttgctt tctcagactt tacgccgact gaatacagta
     8281 ccaaaggaaa gccaaatatt atcgtactaa ccatggatga tcttggttat ggacaacttc
     8341 cttttgataa gggatctttt gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct
     8401 acaaaatagg gatagataaa gccattgaag ctgcacaaaa atcaacgccg acgctccttt
     8461 cattaatgga tgaaggcgta cgttttacta acggctatgt ggcacacggt gtttccggcc
     8521 cctcccgtgc cgcaataatg accggtcgag ctcccgcccg ctttggtgtc tattccaata
     8581 ccgatgctca ggatggtatt ccgctaacag aaactttctt gcctgaatta ttccagaatc
     8641 atggttatta cactgcagca gtaggtaaat ggcacttgtc aaaaatcagt aatgtgccgg
     8701 taccggaaga taaacaaacg cgtgactatc atgacaactt caccacattt tctgcggaag
     8761 aatggcaacc tcaaaaccgt ggatttgatt actttatggg attccacgct gcaggaacgg
     8821 catattacaa ctccccttca ctgttcaaaa atcgtgaacg tgtccccgca aaaggttata
     8881 tcagcgatca gttaaccgat gaggcaattg gcgttgttga tcgtgccaaa acacttgacc
     8941 agccttttat gctttacctg gcttataatg ctccgcacct gccaaatgat aatcctgcac
     9001 cggatcaata tcagaagcaa tttaataccg gtagtcaaac agcagataac tactacgctt
     9061 ccgtttattc tgttgatcag ggtgtaaaac gcattctcga acaactgaag aaaaacggac
     9121 agtatgataa tactattatt ctctttacct ccgataatgg tgcggttatc gatggtcctc
     9181 tgccgctgaa cggggcgcaa aaaggctata agagtcagac ctatcctggc ggtactcaca
     9241 ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg taattatgac aagctgattt
     9301 ccgcaataga tttctacccg acagctcttg atgcagccga tatcagcatt ccaaaagacc
     9361 ttaagctgga tggcgtttcc ttgctgccct ggttgcaaga taagaaacaa ggcgagccac
     9421 ataaaaatct gacctggata acctcttatt ctcactggtt tgacgaggaa aatattccat
     9481 tctgggataa ttaccacaaa tttgttcgcc atcagtcaga cgattacccg cataacccca
     9541 acactgagga cttaagccaa ttctcttata cggtgagaaa taacgattat tcgcttgtct
     9601 atacagtaga aaacaatcag ttaggtctct acaatctgac ggatctacag caaaaagata
     9661 accttgccgc cgccaatccg caggtcgtta aagagatgca aggcgtggta agagagttta
     9721 tcgacagcag ccagccaccg cttagcgagg taaatcagga gaagtttaac aatatcaaga
     9781 aagcactaag cgaagcgaaa taactaaacc ttcatgcggc ggaattttcc gccgccttat
     9841 tgagcgagat agcgatgcac gttacagcca agccctccag ttttcaatgt aatctcaaat
     9901 gtgattactg tttttacctt gaaaaagagt cgcagtttac tcatgaaaaa tggatggatg
     9961 acagcactct gaaagagttc atcaaacaat atatcgcagc gtctggcaat caggtctatt
    10021 ttacctggca aggcggtgaa cccactctgg ctggcctgga ttttttccgt aaagttattc
    10081 actatcaaca acgctatgca ggccaaaaac gtatttttaa tgcattacaa acgaatggca
    10141 ttttattgaa taatgaatgg tgttcctttc tcaaagaaca tgaatttctg gttggtatct
    10201 cgatcgatgg cccccaggag ttacatgacc gttacagacg cagtaattca ggtaacggta
    10261 cttttgcaaa agtgatagca gccatcgagc gtctgaaatc atatcaagtt gagtttaata
    10321 cgttaaccgt cattaataac gttaatgtcc attaccctct tgaggtttat cattttttaa
    10381 aatctatcgg cagtaaacat atgcaattta tcgaattgct cgaaaccggg acgccgaata
    10441 ttgatttcag tggtcatagt gagaacacat tccgtatcat tgatttttct gtgcctccca
    10501 cggcttatgg caagtttatg tcaaccattt ttatgcaatg ggttaaaaac gatgtgggtg
    10561 aaattttcat tcgtcagttt gaaagctttg tcagccgttt tttggggaat gggcatacaa
    10621 gttgtatttt ccaggagtcc tgcaaggata atctggttgt tgaaagtaat ggagacattt
    10681 acgaatgcga ccattttgtc tatccacagt acaaaattgg aaacattaat aaatctgaac
    10741 tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa acggatttca gcgaaatgtc
    10801 agcaatgtgc atataaacct atctgcaatg gcggttgtcc taagcatcgt attactaaag
    10861 taaacaatga gactgtttct tatttttgcg aaggttataa aatccttttt tcaaccatgg
    10921 taccttatat gaacgccatg gttgagttag ctaagaacag agtaccgctt taccacatta
    10981 tggatgttgc aaaacaaatg gagaataatt aataacgctc tcttttaact ccattcttag
    11041 cctccttctt atgaccttaa caaggcaccc gacataaatt cctgtcgggt gtttcgtcat
    11101 catcatcttc ccttgcgaaa atcctggctt acgtggtatg cgtcagggca ttagcaatac
    11161 ccttgattat caatagcata accataacca taaccataac cactgaaaat taaaaatttc
    11221 tatatatttc atattgttaa atgatactta aataaatttc atcagtaaat cattgccata
    11281 gataatcatt ggatggcaca atcaattttc gctaatgata accattccca ttacgttttg
    11341 tatgttgata gcaaagtatc tctgtctgct gaagcccttt tggctgcgta agaataataa
    11401 aacttcagtg ttgttaatca ttattatcct cgcaatgatc cttggcgtgg ttaaaattca
    11461 ggtctggtta aacgactgga ataatgattt tttcaatgcg ctaagccaga aagaaaccga
    11521 taagctttgg cagcttgttc tttggttccc tgccctgctg gggatctttg tattaatatc
    11581 cgtaaacaaa acatggttaa ttaaactact gactattcgc tggcgcgaat ggttaacaga
    11641 ttactacctt aatcgctggt ttgcagataa aaactactac ttcacgcaga tctacggcga
    11701 gcataaaaat acagataacc ctgaccaacg tatcgctgaa gacattctct tactgatcag
    11761 caaaacgctc agtctttcat ttggcttcat ccagtcactt agcatgctga tcacctttac
    11821 cgttattctt tggcaaagcg cgggtacgct ctcattcact gttggtggaa cagaatggaa
    11881 tatccagggg tatatggtct ataccgtcgt gctcattgtg atcggtggaa ctttatttac
    11941 ccataaggtt ggtaagcgta ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac
    12001 atttcgaact aatcttgtgc agcataataa gcaggcagaa ctgattgcgc taagcaatgc
    12061 tgagtctcta caacgccagg agttgcgcga caattttcat accattaaag agaactggca
    12121 tcgtttaatg aatcgtcagc ggtggcttga ttactggcaa aatatttatt cgcgctcgct
    12181 tagcgttctt ccctactttc tgttattgcc gcagtttatt agcgggcaga tcaatctggg
    12241 cggactgatg aaatcgcgcc aggcatttat gctagtatcg aacaatttaa gctggtttat
    12301 ttataaatat gacgaacttg ctgaactggc tgcggttatc gatcgcttgt atgagttcca
    12361 tcaactcact gaacagcgcc ctacgaataa gcctaaaaat tgccaacatg cggtacaagt
    12421 ggctaatgcg agtattcgta cgcctgataa taagatcata ttagagaact tgaactttca
    12481 tgtttcgcca ggcaaatggc tattactgaa aggctactct ggtgcgggaa aaaccacact
    12541 gcttaaaaca ttatcccact gctggccgtg gtttaaaggt gatatttttt ctcctgctga
    12601 cagttggtat gtgtcacaaa caccgttaat caaaaccggc ttactgaaag agattatttg
    12661 taaagcactt cccctgcccg tagacgataa atcgttgagc gaagtactgc atcaggttgg
    12721 tcttgggaaa ttggctgcgc gtattcatga ccacgatcgc tggggagata ttctttccag
    12781 cggcgaaaaa caacgtatcg ccctggcacg attaatttta cgacgtccga aatggatatt
    12841 tcttgacgaa actacctctc atcttgagga acaagaggct atccgcttac tgcgtttagt
    12901 gcgtgaaaaa ctacccacaa gcggcgtcat tatggttaca catcaacccg gtgtctggaa
    12961 cctggccgat gatatttgtg acattagcgc ggttttataa aaacaataaa agccctgctg
    13021 tacggagata taaataaatg aagcgagttc ttattcctgg cgtcatttta tgtggcgctg
    13081 atgtggcgca ggccgtcgat gacaaaaaca tgtacatgca tttttttgaa gagatgacgg
    13141 tctatgctcc tgtccctgta cccgtaaacg gcaacacgca ttacaccagt gaaagcatcg
    13201 agcgtttacc gaccgggaat ggcaatatca gcgatctgct gagaaccaac cctgcggtac
    13261 gcatggattc aacgcaaagt acctcgttga accagggaga tattcgccct gagaaaatct
    13321 ctattcacgg tgcgtcgccc taccagaatg cctatttgat tgacggtatt agtgcaacta
    13381 ataacctgaa cccagcgaat gagtccgatg ccagtagtgc aaccaatatt agcgggatgt
    13441 cacaggggta ttatcttgat gtcagcttac tggacaatgt gacgctttat gacagttttg
    13501 tgccggttga atttggtcgc ttcaatggcg gggtaattga tgcaaagatc aaacgcttca
    13561 acgctgatga tagcaaggtg aaattgggtt atcgcactac gcgttcggac tggttaacat
    13621 cgcatatcga tgagaataac aagagcgcat ttaatcaagg ttcttcagga agtacttatt
    13681 tctctccaga ttttaaaaag aacttttata ccttgtcgtt taatcaggag ctggctgata
    13741 actttggcgt taccgccggt ttatcgcgcc gccagtctga tatcacccgc gcggattatg
    13801 tttcgaatga cggcattgtc gtcggtcggg cacagtataa aaacgttatc gatactgcat
    13861 tgagcaaatt tacctggttt gccagcgacc gctttaccca cgatttaacc ttaaaatata
    13921 ccggctccag ccgtgattat aataccagca ccttcccgca gtctgatcgc gaaatgggta
    13981 ataaatccta tggtcttgca tgggatatgg atacgcaact cgcatgggcc aaactacgta
    14041 ccaccgttgg ttgggatcat attagtgatt atacccgtca cgatcatgac atctggtaca
    14101 ccgaactttc atgtacatat ggtgatatta cagggcgttg tacccgtggc ggattaggac
    14161 acatttccca ggctgtagat aattacacct tcaaaacacg cctggactgg caaaaattcg
    14221 ccgtgggtga tgtttcgcat caaccctact ccggcgcgga atacatctat tccgatgcat
    14281 ggactgaacg ccataaccag tctgaatcct atgtgattaa tgctgcagga aagaaaacta
    14341 accataccat ttaccataaa ggtaaaggca gcctaggaat tgacaactac acgctgtata
    14401 tggcggatca cattagctgg cggaatgtgt cgttaatgcc cggtgtgcgt tatgactatg
    14461 acaactatct gtcaaaccac aatatctccc cgcgctttat gacggaatgg gatatttttg
    14521 ctgatcaaac ctcaatgatt accgccggtt ataaccgtta ctatggcggg aatattcttg
    14581 atatgggatt acgtgatatc cgcaatagct ggacggaatc ggtatcaggt aataaaaccc
    14641 tgacgcgtta tcagaatttg aaaacgcctt ataacgatga actggcaatg ggattgcagc
    14701 aaaaaatcgg taagaacgtt attgcacgcg caaactatgt ttaccgtgaa gcgcatgatc
    14761 aaatcagcaa aagcagtcgt accgacagcg cgactaaaac caccattact gaatataaca
    14821 acgatggcaa aaccaaaacg cattcgttta acctcagttt tgaactggcc gaacccctgc
    14881 atatccgcca ggtagatatt aacccgcaaa ttgtctttag ctatatcaag agcaagggca
    14941 acttgtcgtt aaacaatggt tatgaggaga gcaataccgg tgataaccag gtggtttata
    15001 acggtaatct ggtctcttac gatagcgttc cagtggcaga ttttaataac ccattaaaga
    15061 tctccttaaa catggatttc acgcatcaac cgagcgggtt agtgtgggcg aatacgctgg
    15121 cgtggcaaga agcgcgtaaa gctcgcatta tccttggtaa gacaaatgcg caatacatca
    15181 gcgaatattc agattacaag cagtatgttg acgaaaaact ggatagcagc ctgacctggg
    15241 acacccgctt gtcctggacg ccacaatttc tgaaacaaca aaacctgacg atcagtgccg
    15301 atattctcaa tgtactggat agcaaaaccg ctgttgatac aacgaatacc ggtgtggcga
    15361 cctacgccag tggccgtact ttctggcttg atgtcagcat gaaattttaa tagttcggat
    15421 tttacttaac cgggtaaatt cgcccggttt tcgcatggag ataattatga gaaacctctg
    15481 tttcttactg acgttagtgg caactctgtt gctccccggg cggctgattg ccgccgcctt
    15541 accgcaggat gaaaagttaa ttaccgggca actggacaat ggcttgcgat atatgattta
    15601 tccgcatgct caaccaaagg atcaggtaaa tttatggctg caaattcata ccggttcatt
    15661 gcaggaagaa gacaatgagc gcggcgtggc tcattttgta gaacatatga tgtttaacgg
    15721 cacaaaaaca tggccgggta ataaagtcat cgaaacattt gagtcaatgg gcctgcgttt
    15781 tggtcgcgat gttaatgcct ataccagcta tgacgaaacg gtgtatcagg tgagtttgcc
    15841 gactacgcag aaacaaaatc tgcaacaagt gatggcaatc ttcagtgaat ggagtaatgc
    15901 cgcaaccttt gaaaaactcg aagtagacgc tgaacgtggc gtaattactg aggaatggcg
    15961 tgcccatcag gatgcgaaat ggcgcacctc tcaggcgcgc cgccctttcc tgctggctaa
    16021 tacccgtaat ttagaccgtg aacctatcgg cctgatggat actgtcgcca cggtcacacc
    16081 ggcacaattg cgccaatttt atcaacgctg gtatcaacca aataatatga cctttatcgt
    16141 ggtcggcgat atcgacagta aaaaagcgct ggcgctgata aaggataatt taagtaagct
    16201 tccggctaac aaagcagctg aaaatcgcgt ctggccgaca aaagccgaaa accacctgcg
    16261 ctttaatatc attagtgata aagaaaaccg ggtgaacggc atcgcactct attatcgcct
    16321 gccaatggta caagtgaacg atgagcaaag ctttatcgaa caagctgaat ggagcatgtt
    16381 agttcagctg ttcaatcaac gtctgcagga acgcatacag tcgggcgagt tgaagactat
    16441 ttctggcggc actgcgcgca gcgttaaaat tgcacccgat tatcagtcgc tgtttttccg
    16501 tgtaaatgca cgagacgata atatgcagga tgctgcgaat gcattaatgg cagagttggc
    16561 aaccattgat cagcatggtt tttctgctga agaactcgat gatgtcaaat ctacccgcct
    16621 cacctggctg aaaaatgcgg ttgatcagca agctgaacgt gatttacgta tgctgaccag
    16681 tcgcctggca tccagctcat taaataatac gccgttcttg tcgccggaag agacatatca
    16741 actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt ctggcggaaa aatggcagca
    16801 gttaagaaag aaccaggacg cattttggga gcaaatggta aacaatgagc ttgccgccaa
    16861 aaaagcattg tctcctgcgg ctatcctggc gctggaaaaa gagtacgcca acaaaaagct
    16921 ggcggcttac atcttcccag gcagaaattt atcgttaaca gtagacgctg acccacaggc
    16981 ggaaattagc agcaaagaaa cgctggctga gaatctgaca tcattaacac tttccaatgg
    17041 tgccagggtt attctggcaa aatccgcggg tgaagagcaa aagctacaaa ttactgccgt
    17101 atcgaataaa ggcgatttaa gtttccctgc gcagcaaaaa tcacttatcg cgctggcaaa
    17161 taaagcagta agcggaagcg gcgttggtga gctctcctct tccagcctga aacgctggag
    17221 tgcggaaaat tcggtaacca tgagcagtaa agtcagtggc atgaatacgt tgctctccgt
    17281 tagcgcgcgg actaataacc ctgaacctgg ttttcagttg attaaccagc gaatcaccca
    17341 cagcacgatt aacgacaata tttgggcatc gctacaaaat gctcaaattc aggcgttgaa
    17401 aacgctcgac cagcgtccag cggagaaatt cgcccagcag atgtatgaga cgcgctatgc
    17461 tgatgaccgc acgaaattac tgcaagaaaa tcagattgta cagtttactg ccgcagatgc
    17521 gctggctgcc gatcgccagt tgttttcatc tccagcggat atcacgtttg tcattgtcgg
    17581 taatgtctca gaagacaaac tcgtggcgtt aattacgcgt tacttaggat caatcaaaca
    17641 ctctgattcg ccattagccg caggtaaacc attaactcgc gcgacggaca acgcatcggt
    17701 tactgtaaaa gaacaaaatg aacctgtggc acaggtttca cagtggaagc gttatgattc
    17761 ccggacacct gttaatctgg cgacgcgtat ggcgctcgat gcttttaacg tcgcactggc
    17821 aaaagatcta cgtgttaata ttcgtgaaca ggcatctgga gcatacagcg tttcttctcg
    17881 cctctcggtt gatcctcagg ccaaagatat cagtcatttg ctggctttta cttgtcaacc
    17941 agaacgacat gatgaactgt taacgttagc gaatgaagtg atggttaagc gtctggctaa
    18001 agggatcagt gagcaagaac tgaatgaata ccagcaaaac gttcagcgca gcctcgatat
    18061 ccaacagcgt agcgttcaac aattagcgaa cactattgta aatagtctta ttcaatatga
    18121 cgatcctgca gcatggactg agcaggagca attgttgaaa caaatgacgg tagagaatgt
    18181 taacactgcc gttaaacaat atctttctca tccggtaaat acttataccg gagtattatt
    18241 gccaaaataa taacagcccc gtcaacacat cgttggcggg gattttagca atattcgcta
    18301 tttttatgta ataattttat aaatgcgttc aaaataataa tcaagtacta atagtgatat
    18361 tttaaggtct gatttttacg tgataattca ggagacacag aatgcgcata aaaataacag
    18421 cataaaacac cttaccacca cccaagaatt tcatattgta ttgtttttca atgaaaaaac
    18481 attattcgcg taatatctca cgataaataa cattaggatt ttgttattta aacacgagtc
    18541 ctttgcactt gcttacttta tcgataaatc ctactttttt aatgcgatcc aatcatttta
    18601 aggagtttaa aatggataag aagcaagtaa cggatttaag gtcggaacta ctcgattcac
    18661 gttttggtgc gaagtctatt tccactatcg cagaatcaaa acgttttccg ctgcacgaaa
    18721 tgcgcgacga tgtcgcattc cagattatca atgacgaatt atatcttgat ggcaacgctc
    18781 gtcagaacct ggccactttc tgccagacct gggacgacga aaatgtccac aaattgatgg
    18841 atttatccat taa
//
LOCUS       DADLTD010000070        18732 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLTD010000070 DADLTD010000000
VERSION     DADLTD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.070"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            226..1608
                     /locus_tag="JN701_001900"
     CDS             226..1608
                     /locus_tag="JN701_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7364363.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            1645..2367
                     /gene="folM"
                     /locus_tag="JN701_001901"
     CDS             1645..2367
                     /gene="folM"
                     /locus_tag="JN701_001901"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY7364364.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(2364..2687)
                     /locus_tag="JN701_001902"
     CDS             complement(2364..2687)
                     /locus_tag="JN701_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY7364365.1"
                     /translation="MKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHYIVAS
                     ERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWVLIIC
                     WIKLH"
     gene            2813..3532
                     /gene="rstA"
                     /locus_tag="JN701_001903"
     CDS             2813..3532
                     /gene="rstA"
                     /locus_tag="JN701_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY7364366.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDSITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            3536..4837
                     /gene="rstB"
                     /locus_tag="JN701_001904"
     CDS             3536..4837
                     /gene="rstB"
                     /locus_tag="JN701_001904"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY7364367.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            4913..5842
                     /gene="tus"
                     /locus_tag="JN701_001905"
     CDS             4913..5842
                     /gene="tus"
                     /locus_tag="JN701_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY7364368.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(5839..7242)
                     /gene="fumC"
                     /locus_tag="JN701_001906"
     CDS             complement(5839..7242)
                     /gene="fumC"
                     /locus_tag="JN701_001906"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY7364369.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(7385..9031)
                     /locus_tag="JN701_001907"
     CDS             complement(7385..9031)
                     /locus_tag="JN701_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY7364370.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            9230..10405
                     /gene="manA"
                     /locus_tag="JN701_001908"
     CDS             9230..10405
                     /gene="manA"
                     /locus_tag="JN701_001908"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY7364371.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            10506..12014
                     /locus_tag="JN701_001909"
     CDS             10506..12014
                     /locus_tag="JN701_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7364372.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(12240..13505)
                     /gene="uidC"
                     /locus_tag="JN701_001910"
     CDS             complement(12240..13505)
                     /gene="uidC"
                     /locus_tag="JN701_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY7364373.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHCARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(13544..14917)
                     /gene="uidB"
                     /locus_tag="JN701_001911"
     CDS             complement(13544..14917)
                     /gene="uidB"
                     /locus_tag="JN701_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAY7364374.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVACVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(14914..16714)
                     /gene="uidA"
                     /locus_tag="JN701_001912"
                     /pseudo
     CDS             complement(14914..16714)
                     /gene="uidA"
                     /locus_tag="JN701_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(17102..17692)
                     /locus_tag="JN701_001913"
     CDS             complement(17102..17692)
                     /locus_tag="JN701_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7364375.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(17921..18688)
                     /gene="hdhA"
                     /locus_tag="JN701_001914"
     CDS             complement(17921..18688)
                     /gene="hdhA"
                     /locus_tag="JN701_001914"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY7364376.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         4643 a         4831 c         4497 g         4761 t
ORIGIN      
        1 tggtaataac ctgaccgtca gcgcagatct gtataaataa ctattcagtt actcctataa
       61 ggcggcttga tgccgccttt cgcattttta gtcacaaatt tccccgcgac cattgcatgc
      121 agacacatct cttcgtaaaa tcccgcgctt cagtggcaaa ctccattttt atgcgttttg
      181 ccctaatatt tattcttctt atcacgtttt aatcactgga tatcgatgga aaagaaactg
      241 ggactgagcg cactcaccgc gctggtatta agctcaatgc tgggcgcggg tgttttcagt
      301 ctgccgcaaa atatggcggc agttgccagc ccggcagcac tgctcatcgg ctggggtatt
      361 actggcgctg gcattttatt gctggccttt gccatgctga tcctcacgcg cattcgtccc
      421 gaacttgacg gcggtatctt tacctatgcc cgcgaagggt ttggcgagct aatcggcttt
      481 tgttccgcat ggggatactg gctgtgcgca gtcatcgcca acgtctccta tctggtgatc
      541 gttttttccg cgttaagctt ttttacggac acgccggaat tgcgcctgtt cggcgatggt
      601 aatacctggc aatcgatcgt cggtgcatcg gctttattgt ggattgttca ctttttgatc
      661 ctgcgcgggg tgcaaaccgc tgccagcatt aacctggtgg cgacactggc aaaattgttg
      721 ccgctgggtc tgtttgttgt gctggcgatg atgatgttca aactggatac cttcaagctc
      781 gacttcaccg gacttgtact tggcgtaccc gtttgggaac aggtgaaaaa caccatgctg
      841 atcaccctgt gggttttcat tggtgtggaa ggtgccgtgg ttgtttctgc gcgtgcgcgt
      901 aataaacgtg atgttggcaa agcgacactg ctggcggttc tctccgctct gggcgtttac
      961 ttgctggtaa cgctgctttc gctgggtgtg gtggcacgtc ctgaactggc tgaaattcgt
     1021 aacccgtcaa tggccggtct gatggtggaa atgatgggac catggggcga aatcatcatc
     1081 gctgccagtt tgatcgtttc cgtttgcggc gcgtacttaa gctggaccat catggcggca
     1141 gaagttccgt tcctggctgc cactcataaa gcattcccac gcatttttgc gcgccagaac
     1201 gcacaagctg cgccatccgc ctcactgtgg ttgaccaaca tctgcgtgca aatttgtctg
     1261 gtactcatct ggctgaccgg ttccgattac aacacgttgc tgaccatcgc ctcagaaatg
     1321 attctggtgc cctatttcct ggtcggcgcg ttcttgctga aaatcgcaac ccgtccactg
     1381 cataaagcgg taggtgtcgg tgcctgcatt tatggcttat ggttattgta cgcctctggt
     1441 ccaatgcact tgctgttatc cgttgttctg tatgcgccgg ggctgctggt tttcctgtat
     1501 gcgcgcaaaa cgcatacaca tgataatgta ctgaaccgtc aggaaatggt gctgattggc
     1561 atgctgttaa ttgcctcagt tccggcgacc tggatgctgg tgggataacg ttcatcccat
     1621 cgttggatag caaaggagat aacgatgggt aaagcccagc ccttgccaat attaattact
     1681 ggcggaggtc gtcgcatcgg cctcgccctc gcctggcatt tcattaatca aaagcaaccg
     1741 gtgattgtca gctatcggac gcactatcca gccattgatg gactgattaa ggcaggtgcg
     1801 cagtgtattc aggctgattt ttcgaccaac gacggtgtga tggcgtttgc cgatgaagta
     1861 ctaaaaagca cccatggtct gcgtgctatt ttgcataacg ccagtgcgtg gatggcggaa
     1921 aaactgggtg cgccactggc cgacgtactg gcttgcatga tgcagatcca cgttaatacc
     1981 ccatacctgc tcaaccatgc gctggaaaga ttactgcgtg ggcacggaca cgccgccagc
     2041 gatatcattc actttaccga ttatgtggtg gagcgcggta gcgacaagca tattgcgtat
     2101 gctgcaagca aagcggcact ggataatatg acccgctcgt ttgcccgcaa gctggcaccg
     2161 gaagtgaaag tgaattctat tgctccatcg ctgatcctgt ttaatgaaca tgatgatgcc
     2221 gaatatcgac aacaggcgct gaataaatca ctgatgaaaa ccgcgcctgg cgagaaagaa
     2281 gtgatcgacc tggtcgatta cttacttacc agttgctttg tcaccggacg cagtttccca
     2341 cttgatggcg gtcgtcatct gcgttaatgc agctttatcc agcatataat caacacccat
     2401 gcgcttatcc cccaacacgc aacagaacca acaaaagcgg cgggcaagcg catcattccg
     2461 gtgaaatacc acagcgacac caggtaaaca aaatagggaa taatcgacca catactaaaa
     2521 atgatggttg cgcgtaaggc ttcaatgccg cgttcgctgg caacaatata atgcgcgata
     2581 agcgcaaagg tcgggaaaag tggaatcagc ccggcgatat aataattttt cgtttttgct
     2641 aaaacaccaa tcaacagcac taccagcgca ccgagcgcgg ctttgatttt accttaacac
     2701 ttccataaca agtcatcagt agaatacctg atgaaaactt gtttagaaac gattgatagt
     2761 aagtaaaaac agcgcggtgt attgtgacgt ttttatatct accgtgaatg ttatgaacac
     2821 tatcgtattt gtggaagatg atgcggaagt cggttcactg attgccgcgt acctggcaaa
     2881 acatgatatg caggttaccg tagagccgcg cggcgaccag gccgaagaaa ccattttgcg
     2941 agaaaatccg gatttggtgt tactcgacat catgctacca ggcaaggaca gcattaccat
     3001 ttgtcgtgat ttacgcgcaa agtggtctgg accgattgtt cttctaacct ctctcgatag
     3061 cgatatgaac cacatcctgg cactggaaat gggtgcctgc gactatattc tcaaaacgat
     3121 gccccctgct gttttgctag cgcgtttacg tttgcatttg cgtcagaatg agcaagccac
     3181 actgaccaaa ggtcttcagg aaacgtctct gactccctac aaagccctgc atttcggcac
     3241 gttgaccatc gatcccatca accgcgtagt caccctggct aacactgaaa tctcgctctc
     3301 gacagctgat ttcgaattat tgtgggaatt agctacccat gccgggcaaa tcatggaccg
     3361 cgatgcattg ctgaaaaatt tacgcggcgt cagttatgac ggactggatc gtaacgtgga
     3421 cgtggctatt tcgcggttaa gaaaaaaact gctcgataac gccgcagaac cttatcgcat
     3481 taaaactgtg cgtaacaaag gctatctttt tgcgcctcat gcatgggaat aagcgatgaa
     3541 aaaactgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
     3601 ggttgggttg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
     3661 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcgt gagatccccc cacacgactg
     3721 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
     3781 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
     3841 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
     3901 tggtcctgtt ccttatcttt attatctcca tcagatgcga ttgctggata tcgccctgat
     3961 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
     4021 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tctatgagcg
     4081 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
     4141 ggacaatatc aacgccttga ttgccagcaa aaaacagctt attgacggta tcgctcacga
     4201 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
     4261 cgccgagtcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
     4321 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
     4381 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     4441 acggataaaa acgctcgtgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     4501 cgtgctggat aatttgctca ataacgccct gcggtactgc cattcaacgg ttgaaaccag
     4561 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     4621 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     4681 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     4741 cggtacggtt aattgtgaca ccagcgaact gggtggtgct cgcttctcgt ttagctggcc
     4801 gttatggcat aacatcccgc aatttacctc tgcctgacac tatgcgcacg atggtcaagt
     4861 caccaccatt gtgctataaa ttaaggatgt tgtaactaaa gtggttaata ttatggcgcg
     4921 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctgc
     4981 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     5041 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtgaaac aacatctcgg
     5101 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     5161 cgaaaatcgc agcagcaaag ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     5221 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     5281 gttcgagcat atcgtcacgg ttgagtcaga actccccacc gcggcacgtt ttgaatgggt
     5341 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgctcgctca ccgttctgca
     5401 cgaccccgcc actttacgtt ttggctgggc taataaacat atcattaaaa atttgcatcg
     5461 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
     5521 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     5581 acagaacgcg aagttaaaaa tcaaacgtcc ggtgaaggtg cagccgattg cccgcgtctg
     5641 gtacaaagga gatcaaaaac aagtgcaaca cgcctgccct acaccgctga ttgcactgat
     5701 taatcgggat aatggtgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     5761 caatgtgcag caccgttata aacctcaggc gcagccgctt cgtttgatca ttccacggct
     5821 gcacctgtat gttgcagatt aacgcccggc tttcatactg ccgaccatct gttctggccg
     5881 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     5941 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     6001 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     6061 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     6121 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     6181 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     6241 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     6301 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     6361 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     6421 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     6481 ggtaatgact gccagttcat ctgctacgcg acgcgcatac tccggatggg tatttagtcc
     6541 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     6601 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     6661 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     6721 tttttcactc agtgtctgcg tcagggtttt aagctgcgga atgagttgct tgcgcagcgc
     6781 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     6841 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acgccgccga gtaattcact
     6901 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     6961 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     7021 ccgaatggcg ctcgctttct cttcagacaa caagcctaaa tcttcattaa cttttgccgc
     7081 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     7141 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     7201 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctgt
     7261 ttaatttttt ctttctgttt tgctttcgct aagcaacttt tagctcactt attatttact
     7321 atttgataac aagtgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     7381 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     7441 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     7501 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     7561 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     7621 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     7681 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     7741 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtactgcg
     7801 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccga
     7861 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     7921 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     7981 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     8041 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     8101 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcgtat gtcgtgagcg aagtatttac
     8161 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagcaattct ttttccagtt
     8221 ccacatcgcg gaatgcctga ccgtgctcat tcccttccgt tggcagttcg tcatagtatt
     8281 tagcggaagc cagtttcact gttttaaggt tagtttctgc agaagttcca ccaattacga
     8341 acgcaatatg atacggagga caggccgccg tacccagcgt gcgcatcttc tcaaccaggt
     8401 aatttttcag tttccccggc gttagtaacg ctttggtttc ctgatagaga tacgtcttgt
     8461 ttgccgaacc accacctttg gcgatacaga ggaatttgta ctcgtcgcca tcaacggcat
     8521 aaagatcgat ctgcgctggc agattggtgc cggtattcac ctctttatac atatccagcg
     8581 gcgcgttttg cgagtagcgc aaattatctt caatataagt gttatagaca ccgcgcgcca
     8641 gcgccgcttc atcacagcca ccggtccata cacgctgccc ttttttacca acaataatcg
     8701 cagtgccggt atcctgacag gttggcagaa cacctttcgc cgcgatgtcg gagttgcgca
     8761 ggaattgcag cgccacatat ttatcatttt cgctggcttc cgggtcacgc agaatgtcgg
     8821 ccacctgttg ttggtgagcc ggacgcagca taaatgacgc atcatgaaac gcctgacgcg
     8881 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     8941 cgctaacgtg ttcgctggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     9001 gagcctgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     9061 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacactt
     9121 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     9181 tcttgcttta atagtgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     9241 cattaactca gtgcaaaact atgcctgggg cagcaaaacg gcgttgactg aactttatgg
     9301 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     9361 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     9421 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     9481 gttcaaagta ttatgcgcag cacagccgct ctccattcag gttcatccaa acaaacgcaa
     9541 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     9601 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     9661 gatgaacgcg tttcgtgagt tttccgagat tgtctcccta ctccagccag tcgcaggtgc
     9721 acatccggca attgctcact ttttacaaca gcctaatgcc gaacgtttaa gcgaactgtt
     9781 cgccagcctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     9841 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     9901 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     9961 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
    10021 agtgatggcg aactccgata acgtgctgcg tgcgggtctg acgcctaagt acattgatat
    10081 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
    10141 gccggtgaaa caaggtgcag aactggactt cccgattcca gtggatgatt ttgccttctc
    10201 gctgcatgac cttagtgata aagaaaccac cattagccag cagagtgccg ccattttgtt
    10261 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
    10321 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
    10381 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
    10441 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
    10501 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
    10561 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
    10621 aggcgaacgc gcaactaaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
    10681 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
    10741 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
    10801 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
    10861 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
    10921 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
    10981 tcaagccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
    11041 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
    11101 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
    11161 ccgacggttc cagcacgctg gcaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
    11221 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
    11281 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
    11341 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
    11401 tcggccagat tgatggtgaa gcctggcatc agttcagcca gcaatataac gcgcaaactc
    11461 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
    11521 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactgtcg
    11581 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
    11641 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
    11701 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag tttatgactc
    11761 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
    11821 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
    11881 tcaccaccag cctgcaatat actaacggtc agataacgtt aaatgggcag aaaatgtcgt
    11941 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
    12001 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcacttcggc gttttcatct
    12061 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaat
    12121 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
    12181 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
    12241 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
    12301 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
    12361 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgtgccttca
    12421 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagt
    12481 gtaaattgcg gcgttaaacg atagttgacg cccaattgcc aggactgcat tccttcacgg
    12541 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
    12601 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
    12661 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
    12721 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
    12781 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
    12841 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcaatgttta
    12901 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
    12961 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
    13021 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
    13081 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
    13141 agcaggctat tttcacaggg ttttattttt actgccccca gggcaaagcc aaaactttta
    13201 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
    13261 tatgccccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgaacaat
    13321 cctctctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atccgccctg
    13381 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccgca
    13441 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    13501 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    13561 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    13621 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    13681 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttgc acctgattgg
    13741 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    13801 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    13861 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    13921 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    13981 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    14041 taccgctcct cgcgaccatc cccggcacca gcggtgccga tgccacagta ccaaccaagt
    14101 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgctcgtaga
    14161 acaacgacga ggcgctgacc gcaaaggtcg aaatcagcac acacagcgca ccgatgcaca
    14221 acataaacag cgggcgattc cgtttcaggg tttgcagact gatcttcaat gacggctgcg
    14281 caacgatacg taccacattc tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    14341 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    14401 agttcttaat gctcggccct atcagaaatg ccaggcagac aaaggtcaat gaagcggcaa
    14461 tcccacgagc cgcgcccaga cgggcgcggg attgtggttg ttgggtcatc gcggtagcaa
    14521 gtgaaccata aggaatattc accaggctat agcaaagccc gaggcccatg taggtcaaat
    14581 atgcatacac tactttgctg ctatggctcc agtcggtcgg tacccagaat accagcacgc
    14641 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    14701 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatcg aatacccgca
    14761 ccaataacag catggtgccc gccgcagcgg caccgacgca agcgacgtcg gtgtagtaac
    14821 tcaacaggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    14881 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    14941 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    15001 gcggtcgcgg gtgaagatcc ccttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    15061 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    15121 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    15181 tgagtgcagc ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    15241 tttctcctgc caggctagaa gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    15301 gctttggaca taccatccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    15361 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    15421 gagtttacgc gttgcttccg ccagtggcgc gaaatattct cgtgcacctt gcggacgggt
    15481 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    15541 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    15601 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    15661 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccact cgagcatctc
    15721 ttcagcgtaa gggtaatgcg aggttcggta ggagttggcc ccaatccagt ccattaatgc
    15781 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    15841 acgaccaaag ccagtaaagt agaacggttt gtggttgttc gtccttcact gccactgacc
    15901 ggatgccgac gcgcagcggg tagatatcac actctgtctg gcttttggct gtgacgcaca
    15961 gttcatagag ataaccttca cccggttgcc agaggtgggg attcaccact tgcaaagtcc
    16021 cgctggtacc ttgtccagtt gcaaccacct gctgatccgc atcacgcagt tcaacgctga
    16081 catcaccatt tgctaccacc tgccagtcaa cagacgcgtg gttacagtct tgcgcaacat
    16141 gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagacc attacgctgc
    16201 gatggatccc ggcatagtta aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt
    16261 catcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg ttcacacaaa
    16321 cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct tcaaatggcg
    16381 tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg ccgtaatgag
    16441 tgaccgcatc gaaacgcagc acgatacgct ggcccgccca acctttcggt ataaagactt
    16501 cgcgctgata ccagacgttg cccacataat tacgaatatc tgcatcggcg aactgatcgt
    16561 taaaactgcc tggcacagca attgcccggc tttcttgtaa cgcgctttcc caccaacgct
    16621 gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg agttttttga
    16681 tttcacgggt tggggtttct acaggacgta acataaggga ctcctcattc aaataaaaat
    16741 actggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag ttatctgtga
    16801 gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa aaaaccaaaa
    16861 tctgcaatac gcacagcgtg atatagatcg cattaatctt taaaacagcg ttgacagaag
    16921 ccataaaata agcaatcgaa cgttcgctcg ggcatcttga gaacgataaa ctctcatgac
    16981 atatggctac ggtgaatatt ttggggggga attctccgat cccggctaga ttgtattttg
    17041 tacagggaaa ttaaagtatg aaacgcgggc acagttatca ccagcccgcg aaatgagagg
    17101 atcaggatgc ggttaagata ccgccaatca tcgcgcgtaa tccctgagca aacgacgctt
    17161 cacgcgccag atcttccgct tcgatatcgg ccagtacacc gtaggtcaga tccagcagta
    17221 aacgtgaagt cattgacgtg ttgatgtctg ggcttatctc gcctttttgc tgggcatcac
    17281 gcatccgctg ggcaacaaat tcagtgatcg tcatatgctt atttttaagc atggcggcga
    17341 cctgtgggtt acgcatccct tccgccataa tttcgaccac cagcgcccgt tgcccaaagg
    17401 cttcatgggt gagagagaca atagactcga ccatatagtc aacaaaatga atcccttcaa
    17461 tcggttcccg gaaacgggcc agcgccctct cctggtcctg taagataatc gcctgaatca
    17521 aggcttcttt ggagatgaaa tggtgataaa gcgtcccggg actaatggcg caggatttac
    17581 agatggcttt catcgaggca ctgtgaaatc cattttctga aaaaatctct ctggcagcat
    17641 tgaggatccg ggtccgtgtc ggttgtgctt cagtctgcat gttatccatc attaaaccat
    17701 tatagttagc gtgaataact agtttaccgc gttgtcaggt gagcgcctat cattgctgtt
    17761 gaatagtgcg aaggcacact ctattcgtat gcttttcatc cagctattgg ttaaccaata
    17821 gccgtgattt ggctgccagt tgtttagcag agacgttgat gaattgcaca cgctatcccg
    17881 gtaaacaaca catacggctg tttaccggtt cgttagtgta ttaattgagc tcctgtaccc
    17941 caccaccgga gacggtgaga atttgtccgc ttacccagct ggctgcaggc gagcaaagga
    18001 acagcgccgc gttagcaata tcttgcggtt gacccagacg tctgatcggc gtgtgctgta
    18061 acattttttg ttcaatttct ggtgtaataa cggatttcag ggcatcggtt aatattgccc
    18121 ccggcgcaat gccattcacc cgaatatttt tttcacccag gtcaaatgcc atatttctga
    18181 ccagatgact ggccgcagct ttagatgatg cataggaagt catgtttata tttttatttt
    18241 ctgccgccat agaagtgatg gtcagaataa cgccaccgcc atttttttcc atttctggcg
    18301 caacaagttg tgacagatgg aaaaaagaaa acacattcag ttcataagca cggcgaaaat
    18361 ccgccattgg catatcaaac ggtttaggtc caccgccacc ggcgttgtta accagaatat
    18421 caactttacc cagcttactg atagcaaagt ctgccagtgc agagagttcc tgttcggaag
    18481 taatatcaca acggcaggca aatgcctgcc cccccagttg ttgaatttcg tctacaacat
    18541 ggttagctgc gtcggcgtta atatcactga ccaccacaga tgcgccagct gtcgcgaatg
    18601 taatggcgat ttctttacca atacctgcac ccgcacctgt gatgatggcg cattttccgt
    18661 cgagtctcag gttgtcagaa ttaaacacat aaacctcctg tgaacttcag taaaaataaa
    18721 aggcaccgcc tg
//
LOCUS       DADLTD010000071        18503 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLTD010000071 DADLTD010000000
VERSION     DADLTD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.071"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..891
                     /locus_tag="JN701_001915"
     CDS             <1..891
                     /locus_tag="JN701_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7364377.1"
                     /translation="WFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFIT
                     IGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTM
                     WGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVT
                     DWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTS
                     CRGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTA
                     AGALAYIFLVRQPINDPRKD"
     gene            complement(940..2595)
                     /gene="tsr"
                     /locus_tag="JN701_001916"
     CDS             complement(940..2595)
                     /gene="tsr"
                     /locus_tag="JN701_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7364378.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2714..3160)
                     /gene="hpaR"
                     /locus_tag="JN701_001917"
     CDS             complement(2714..3160)
                     /gene="hpaR"
                     /locus_tag="JN701_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY7364379.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3432..4721
                     /locus_tag="JN701_001918"
     CDS             3432..4721
                     /locus_tag="JN701_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY7364380.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4718..6184
                     /gene="hpaE"
                     /locus_tag="JN701_001919"
     CDS             4718..6184
                     /gene="hpaE"
                     /locus_tag="JN701_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7364381.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRDHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6186..7037
                     /gene="hpaD"
                     /locus_tag="JN701_001920"
     CDS             6186..7037
                     /gene="hpaD"
                     /locus_tag="JN701_001920"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY7364382.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7047..7427
                     /locus_tag="JN701_001921"
     CDS             7047..7427
                     /locus_tag="JN701_001921"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY7364383.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7538..8341
                     /gene="hpaH"
                     /locus_tag="JN701_001922"
     CDS             7538..8341
                     /gene="hpaH"
                     /locus_tag="JN701_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY7364384.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8352..9140
                     /gene="hpaI"
                     /locus_tag="JN701_001923"
     CDS             8352..9140
                     /gene="hpaI"
                     /locus_tag="JN701_001923"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY7364385.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVCSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9315..10691
                     /gene="hpaX"
                     /locus_tag="JN701_001924"
     CDS             9315..10691
                     /gene="hpaX"
                     /locus_tag="JN701_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY7364386.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10701..11587
                     /gene="hpaA"
                     /locus_tag="JN701_001925"
                     /pseudo
     CDS             10701..11587
                     /gene="hpaA"
                     /locus_tag="JN701_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11838..13400
                     /gene="hpaB"
                     /locus_tag="JN701_001926"
     CDS             11838..13400
                     /gene="hpaB"
                     /locus_tag="JN701_001926"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HAY7364387.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13418..13930
                     /locus_tag="JN701_001927"
     CDS             13418..13930
                     /locus_tag="JN701_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY7364388.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14323..16473
                     /gene="btsT"
                     /locus_tag="JN701_001928"
     CDS             14323..16473
                     /gene="btsT"
                     /locus_tag="JN701_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY7364389.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKHTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16604..16807
                     /locus_tag="JN701_001929"
     CDS             16604..16807
                     /locus_tag="JN701_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7364390.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16818..17774
                     /gene="yjiA"
                     /locus_tag="JN701_001930"
     CDS             16818..17774
                     /gene="yjiA"
                     /locus_tag="JN701_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY7364391.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17876..>18503)
                     /locus_tag="JN701_001931"
     CDS             complement(17876..>18503)
                     /locus_tag="JN701_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase"
                     /protein_id="HAY7364392.1"
                     /translation="PGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTG
                     SLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVS
                     NWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWD
                     VVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWLTKQDVEAHKES"
BASE COUNT         4225 a         5125 c         4936 g         4217 t
ORIGIN      
        1 tggttcaaca tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt
       61 ggcgttgccg taagcccacc gattctggcg gcgatgatgc tggtgatggg ctggcgcggg
      121 atgtttatta ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat
      181 cgcaaccgcg agcacgtaga actgactgcc gttgagcaag cttatctcaa tgcaggtagc
      241 gtcaatgccc gccgagatcc gctcagtttt gccgaatggc gcagcctgtt ccgtaaccgt
      301 acaatgtggg gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg
      361 gcctggcttc ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg
      421 atgacggcta tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttacc
      481 gactggctgg tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattgcc
      541 gggatgttct gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgacg
      601 gcggttctgc tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgccgg
      661 ggcttgatcc acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac
      721 tttgccagct tcatctgcgc ctcttttgcg ccgatcatta ctggttttat tgttgatacc
      781 acccactcat tccgtctggc actaatcatc tgcggttgcg tcaccgcagc gggggcactg
      841 gcgtacatct tcctggttcg tcagccgatc aacgacccac gtaaagatta atctccttat
      901 gcccgataac attttgctta tcgggcattt tcatggcgat taaaatgttt cccagttctc
      961 ctcgctatct gccacggcca ttttacgcgg cgcagctggc gtcacggttt ttaccacagc
     1021 cgatgtttca cgctgctgtt gctgaatccg gaacactgcc actgcttcgg tcaggcgact
     1081 ggcctgctct tccagcgcgg cggcggcagc ggcagactct tccaccagcg cggcgttctg
     1141 ttgagttacc cggtccatct cagcaaccgc taagccaacc tgatcgatac cacggctctg
     1201 ctcatcagaa gcagaagcaa tttcgcccat aatgtccgtc acgcgggtca cggcgctgac
     1261 aatctccgcc attgtttccc cggcgctttc gaccagcgta gagccaacat ccactttccc
     1321 caccgagtct tcaatcaggc ttttaatttc acgagccgcc tgggcgctgc gctgggccag
     1381 attacgcact tctcccgcga ccaccgcaaa accgcgccct tgctcacccg cacgcgcagc
     1441 ctcaaccgcc gcgttcaaag ccagaatatt ggtctggaag gcaatgccgt caattacgct
     1501 gataatatcg gcgattttct gcgaactggt ggagatatcg cgcatagtct gcaccacgtt
     1561 atctaccact ttgccgccgc gttgcgccgt ttcagaagca cttaacgcca gatggctggc
     1621 ctggcgcgca ttttcggcgt tctgcttcac cgttgcggtc agttgctcca tgctggctgc
     1681 cgtctcttcc agcgaagcgg cctgttgctc ggtgcgcgaa gagagatcgt tattgccggt
     1741 ggcgatttcg ctggcaccgc tatagatggc attggccccg ttgcgcacat caccgacggt
     1801 acgcatcagc tctccctgca tatggcgcaa actctctgcc agttgcccca tctcattaga
     1861 gccatccacc tcaatcggtt tcaccagatc gccgcctgca atatgacgaa tgctgtcaat
     1921 caggcgattc attggcgcta ccagcgaggc tttaataccg aaccagacgg cgaagatgac
     1981 cgccagtacg acgatcatca cgcccaccag aatccacatc gcctggctgt aggaggcatt
     2041 gttatcgctg acggcgatat catagagccg atcgttttgc tccatgtaag ccacatactg
     2101 cttctcgaaa ccgtcctgat atccctgggt cggctgatca aagaactcgt tgattttgcc
     2161 tgcacctaac agttggatca gctccgccag cgcattgtga taaatatcgt aattacgttt
     2221 gatctctgcc gctgcggcgg tgctctgacg cgggtcacgc ggcaacgctt cgtaatccgc
     2281 ccagtttttt tccgcctgtt tcagcgaaat actggcactc tgcatcagct cagcaacggt
     2341 tgaaccgcta ccaatattat tctgatccat catgtagcgg atacccgcgc ggttgagggt
     2401 gttacgcgtc tgcaacaacg cgacccagct gccattcagc gtggattgct gctggcgaat
     2461 ggtttgtaaa acagtgaaat tttctttgtc attctttaag gcattaaaga acagaccgcc
     2521 tgatgtcagt tgtaaaaggc caaaaacggc caaaaccagc agtaagctgg tcacgatctt
     2581 gatacgtttt aacatggttt ctctttcctg tggaccgatg caagattttc ggcctggaaa
     2641 gcaaaaactt tatgaaaagg tgtaacccgc catccatgga aaaaccaggc gctggttatc
     2701 agatactaaa aagttattca ttatcgcccg ggatggcttc ttgtcgggaa ttgcccagag
     2761 caataaattc ctctaacaga tgggttaact gttgcatttt ctcggcagta aactgagctt
     2821 caatctgccg ataggcttct tcaatctgtg tctgagcgcg gttatacagc gcctgcccct
     2881 ctttggttag cgagatatag agcttccgct gatcattgat cggctttaat cgcaacacta
     2941 aaccgtcgcg ttccatccgc gtgagaatcc cggtcaggct tgggcgcaaa atgcaggcgc
     3001 gatacgccag atcgtgaaaa tccattgatg ggctttccgc cagaatacgc acgatgcgcc
     3061 actgttgctc agtcaaatta tgccgtttaa cgattgggcg aaaataactc atcgccgctt
     3121 ctcgcgcctg gagcaacgca atggttagtg agtcgtgcat tatctttccc ctgaaaaatt
     3181 caaaatcatt aatatagaaa cagttaatcg tcacgttacc gggactgcgc tgcccctgca
     3241 attcctgatt ttatcaaaat tgatgatatt ttattaactt actgatattt aatattttat
     3301 tatctttaaa tgtaaatagt ttgttaatta gatcacattt acatcactta cttttgccaa
     3361 actacgcaat cgggaataaa agcaatcatt aacatattaa tgattaagat aatcgaatcc
     3421 gaggagtggt aatgaaaggc actatcttcg ccgtagcgtt gaaccatcgc agccaacttg
     3481 atgcatggca ggaagcgttc cagcaatccc cctacaaagc cccgcctaaa actgcggtct
     3541 ggtttattaa accgcgcaat acggtgattg gttgcggtga accgattccc tttccacagg
     3601 gtgaaaaggt actgagcggt gcgactgttg cgctgattgt gggaaaaacg gcgacgaaag
     3661 tacgtgaaga agatgcggca gagtacatcg ccggatatgc gctggctaac gacgtcagcc
     3721 tgccggaaga gagcttttac cgcccggcaa tcaaagcaaa atgtcgtgat ggattctgcc
     3781 ccattggcga aaccgtggct ctcagcaatg tcgataatct gaccatctat accgagatca
     3841 acgggcgtcc tgccgatcac tggaataccg ccgatttaca acgtaacgcc gcgcagttgc
     3901 tgagcgccct gagcgaattt gccacgctga atccaggcga cgccattctg ctcggcacgc
     3961 cacaggcgcg cgtggaaata cagccaggcg atcgcgttcg tgttctcgca gaaggtttcc
     4021 cgccgctgga aaatccggta gtggacgaac gtgaagtgac cacgcgcaag agcttcccaa
     4081 cgcagccaca cccgcacggc acgctgtttg ccctcggcct gaactacgcc gaccacgcca
     4141 gcgaactgga atttaagcca ccggaagaac cgctggtatt cctgaaagcg ccaaataccc
     4201 tcactggcga taaccagacc tccgtgcgtc caaacaatat tgaatacatg cactatgaag
     4261 cggagctggt ggtagttatt ggcaaacagg cgcgtaacgt cagcgaagcc gatgccatgg
     4321 attatgtcgc gggctacacc gtgtgtaacg actacgccat tcgtgactat ctggaaaact
     4381 actaccgccc taacctgcgg gtaaaaagcc gcgacggact gacgccgatg ctttcaacca
     4441 tcgtgccgaa agaggcgatc ccggacccgc ataatctgac ccttcgcacc ttcgtcaacg
     4501 gcgagttacg ccagcaaggc accaccgccg atctgatctt cagcgtgccc ttcctgatcg
     4561 cctacttaag tgaatttatg accctgaatc cgggcgacat gatcgccacc ggcacaccca
     4621 aaggcttatc tgacgtggtg cctggcgatg aagtagtggt ggaagtagaa ggcgtgggcc
     4681 gcctggtgaa ccgaattgtg agtgaggata cagcgaaatg aaaaaagtaa atcattggat
     4741 caacggcaaa aatgttgcag gtaacgacta cttccagacc accaatccgg caacgggtga
     4801 agtgctggcg gatgtggcct ctggcggtga agcggagatc aatcaggcgg tagcggcagc
     4861 gaaagaggcg ttcccgaaat gggccaatct gccgatgaaa gagcgtgcgc gcctgatgcg
     4921 ccgtctgggc gatctgatcg accagaacgt gccagagatc gccgcgatgg aaaccgcgga
     4981 caccggcctg ccgatccatc agaccaaaaa tgtgttgatc ccacgcgctt cccacaactt
     5041 tgaatttttc gcggaagtct gccagcagat gaacggcaag acctatccgg ttgacgacaa
     5101 gatgctcaac tacacgctgg tgcagccggt gggcgtttgt gcgctggtat cgccgtggaa
     5161 cgtaccgttt atgaccgcca catggaaggt cgcgccgtgt ctggcgctgg gcaataccgc
     5221 ggtactgaaa atgtcggaac tctccccgct gaccgctgac cgcctgggtg agctggcgct
     5281 ggaagccggt attccggcag gcgtgctgaa cgtggtacag ggctacggcg caaccgcagg
     5341 ggatgcgctg gttcgtgatc atgacgtacg tgccgtgtcg ttcaccggcg gtacggccac
     5401 cgggcgcaac atcatgaaaa acgccgggct gaaaaaatac tccatggaac tgggcggtaa
     5461 atcgccggtg ctgatttttg aagatgccga tattgaacgc gcgctggacg ccgccctgtt
     5521 caccatcttc tcgatcaacg gcgaacgctg caccgccggt tcacgcatct ttattcagca
     5581 aagcatctac ccggaattcg ttaaacgctt tgccgaacgc gccaaccgtc tgcgcgtggg
     5641 cgatccgaac gatccgaata cccaggttgg cgcgcttatc agccagcagc actgggaaaa
     5701 agtctccggc tatatccgtc tcggcattga agaaggcgcc accctgctgg cgggcggccc
     5761 ggataaaccg tctgacctgc ctgcacacct gaaaggcggc aacttcctgc gcccaacggt
     5821 gctggcagac gttgataacc gtatgcgagt cgcccaagaa gagattttcg ggccggtcgc
     5881 ctgcctgctg ccgtttaaag acgaagctga aggcttacgc ctggcaaacg acgtggagta
     5941 cggcctcgcg tcgtacatct ggacacagga tgtcagcaaa gtgttacgcc tggcgcgtgg
     6001 cattgaagct ggcatggtgt tcgtcaacac ccagaacgtg cgtgacctgc gccagccatt
     6061 tggcggcgta aaagcctccg gcaccgggcg tgaaggcggt gagtacagct tcgaagtgtt
     6121 cgcggaaatg aagaacgtct gcatttccat gggcgaccat ccaattccga aatggggagt
     6181 ctgatatggg taagttagcg ttagccgcca agatcacgca cgtaccgtcg atgtatctct
     6241 ctgaactgcc agggaaaaac cacggttgcc gccagggcgc gatcgacggg cataaagaga
     6301 tcagcaagcg ttgccgggaa atgggcgtcg ataccattat cgttttcgat acccactggc
     6361 tggtcaacag tgcttatcac atcaactgtg cagaccattt tgaaggcgtc tacaccagca
     6421 acgagctgcc gcattttatc cgcgatatga cctacaacta cgagggcaac ccggagttgg
     6481 ggcagcttat tgccgatgaa gccttaaagc tcggcgtgcg ggcaaaagcg cacaacattc
     6541 ccagcctgaa actggaatac ggcacgctgg tgccgatgcg ctacatgaat gaagacaagc
     6601 acttcaaagt ggtctccatt tcggctttct gcacggttca cgattttgcc gacagccgca
     6661 agctgggcga agcgattctg aaagcgatcg aacagtacga cggcaccgtg gcggtccttg
     6721 ccagcggttc gttatcgcac cgctttattg acgatcagcg tgcagaagaa gggatgaaca
     6781 gctacacccg cgagttcgac cgccagatgg acgagcgcgt ggtgaagttg tggcgcgaag
     6841 gccagttcaa agagttctgc aatatgctgc cggagtacgc cgactactgc tacggcgaag
     6901 gcaatatgca cgacacggtg atgctgctgg ggatgctcgg ctgggataaa tacgacggca
     6961 aggtggagtt tatcaccgag ctattcccaa gctctggcac cggtcaggtt aacgctgttt
     7021 tcccgcttcc cgcgtaagga cgttttatgc cgcactttat cgttgaatgc agtgacaaca
     7081 tccgcgaaga agccgacctg ccggggttgt tcgccaaagt gaatccgacg ctggcagcca
     7141 cgggtatttt tccgctggcg ggtattcgca gccgcgtgca ttgggtcgat acctggcaga
     7201 tggccgacgg gcagcgtgat tatgccttcg tgcatatgac gttgaaaatc ggcgcaggtc
     7261 gcagcctgga aagccgtcag caggcgggtg aaatgctgtt tgaactgatt aaaacgcact
     7321 tcgccgccct gatggagagc cgcctgctgg cgttgtcgtt tgagattgaa gagctgcatc
     7381 cgacgctgaa ttttaaacaa aacaacgtgc acgcattgtt taagtagcgc gcagattgcc
     7441 cggtggcgct gcgcttaccg ggcctacaaa accccaaacc gtacaccgta ggccggataa
     7501 ggcgcagccg catccggcaa tgccacagga tatcgctatg ttcgataaac acacccacac
     7561 cctgatcgcc cagcgtctgg atcaggcaga aaaacagcgc gaacagatcc gcgcgatctc
     7621 gctggattac ccagagatca ccatcgaaga cgcttacgcg gtgcagcgcg aatgggttcg
     7681 cctgaaaatc gccgaaggcc gtacgctgaa aggccacaaa atcggtctga cctcaaaagc
     7741 aatgcaggcc agctcgcaga tcagcgaacc agattacggt gccctgctgg atgacatgtt
     7801 cttccacgat ggcagcgata tcccgaccga tcgctttatc gtgccgcgca ttgaagtgga
     7861 gctggctttc gtgctggcaa aaccgctgcg tgggccaaac tgcacgctgt tcgacgtcta
     7921 caacgccacg gactacgtga tcccggcgct ggagctgatc gacgctcgct gccacaacat
     7981 cgatccggaa acccagcgcc cgcgcaaagt gttcgacacc atttctgata acgccgccaa
     8041 cgccggggtg atcctcggtg gtcgtccgat caaaccagac gaactggatc tgcgttggat
     8101 ctccgccctg atgtatcgca atggcgtgat tgaagaaacc ggcgtcgccg ctggcgtgct
     8161 gaatcatccg gcaaacggcg tggcctggct ggcgaacaaa ctcgcaccgt atgacgtaca
     8221 actggaagcc gggcaaatca ttctcggtgg ttcgttcacc cgcccggttc cggcgcgtaa
     8281 gggcgacacc ttccacgtcg attacggcaa catgggttcc attagctgcc gctttgttta
     8341 aggagagaac gatggaaaac agctttaaag cggcgctgaa agcaggccgt ccgcagatcg
     8401 gattatggct ggggctgagt agcagctaca gcgcagagtt actggccgga gcaggattcg
     8461 actggttgtt gatcgacggt gagcacgcgc cgaataacgt gcaaaccgtg ctcacccagc
     8521 tacaggcgat tgcgccctac cccagccagc cggtagtacg tccgtcgtgg aacgatccgg
     8581 tgcaaatcaa acaactgctg gacgtcggca cacaaacctt gctggtgccg atggtacaaa
     8641 acgctgacga agcccgtgaa gcggtacgcg ccacccgtta tccccccgcc ggtattcgcg
     8701 gtgtgtgcag tgcgctggcc cgcgcctcgc gctggaatcg tattcctgat tacctgcaaa
     8761 aagccaacga tcaaatgtgc gtgctggtgc agatcgaaac gcgtgaggca atgaagaact
     8821 taccgcagat tctggacgtg gaaggcgtcg acggcgtgtt tatcggcccg gcggatctga
     8881 gcgccgatat gggttatgcc ggtaatccgc agcacccgga agtacaggcc gccattgagc
     8941 aggcgatcgt gcagatccgt gaatcgggca aagcgccggg gatcctgatc gccaatgagc
     9001 aactggcaaa acgctatctg gaactgggcg cgctgtttgt cgccgtcggc gttgacacca
     9061 ccctgctcgc ccgcgccgct gaagcgctgg cagcacggtt tggtgcgcag gccaccgccg
     9121 tgaagcccgg cgtgtattaa tgccggatgc gctgcactta tccggcctac actcgcacca
     9181 aaccgtaggc cggataaggc gtttacgccg catccggcaa aaagccgtac caaatcgcgg
     9241 accggataag gcgttacgcc gcatccgcca taaaccttgt cgtaccctac aaaaatccca
     9301 ttagaggaag aaaaatgagc gacacctcac ctgccatacc ggagagtatc gatccggcga
     9361 atcagcataa agcgctgact gccggacaac aggcggttat taagaagcta tttcgccgcc
     9421 tgatcgtctt tctgttcgtg ctgtttatct tctcgttcct tgatcgcatc aacatcggct
     9481 ttgccggact cacgatggga cgcgacctcg gtctgagcgc caccatgttt ggcctcgcta
     9541 ccaccctgtt ctatgccgct tatgtcatct tcggcattcc cagcaacatt atgctgagta
     9601 ttgtcggtgc gcggcgctgg atcgccacca tcatggtgct ctggggcatc gcctctactg
     9661 ccaccatgtt tgccactggc cccaccagct tgtacgtact gcgtatactg gttggcatta
     9721 ccgaagccgg ctttctgcct ggtattctgc tgtatttaac cttctggttt ccggcctatt
     9781 tccgcgcccg tgccaacgcc ttgtttatgg tagcaatgcc ggtaacgaca gcgttgggct
     9841 cgatcgtttc cggctacatt ttgtcgctgg atggcgtcat ggcattaaaa ggctggcagt
     9901 ggctgttttt gctggaaggc ttcccgtcgg tattactcgg cgtcatggtg tggttctggc
     9961 ttgatgactc accggacaaa gctaagtggc tgacgaaaga agacaaaaaa tgcctgcaag
    10021 agatgatgga taacgatcgt ctgacgctgg ttcagccaga gggagccatc agccaccacg
    10081 ccatgcaaca acgcagtatg tggcgggaga tcttcactcc ggtggtgatg atgtataccc
    10141 tggcgtattt ctgcctgacc aacacactta gtgcgatcag catctggaca ccgcagatcc
    10201 tgcaaagttt taatcagggc agcagtaata tcaccatcgg cctgctggcc gccgtaccgc
    10261 agatttgtac cattctcggg atggtctact ggagccgtca ctcagatcgc cgccaggaac
    10321 gaaggcatca caccgccctt ccttatttgt tcgctgccgc aggttggtta ctggcttcgg
    10381 caactgatca caacatgatc cagatgctgg ggatcattat ggcttcgacc ggatcattca
    10441 gcgcaatggc gattttctgg acaacaccgg atcagtccat cagcctgcgg gcacgagcga
    10501 tcggtattgc ggtgatcaac gccactggca acattggttc agcgttaagt ccgtttatga
    10561 tcggctggtt gaaagatttg accggcagct ttaacagtgg attgtggttt gttgccgcgc
    10621 tgctggtgat tggtgcgggg attatctggg cgattccaat gcagtcctcc cgtccgcgag
    10681 cgaccccgta aggaacgacg atgtgtgacc gtcagattgc caatattgat atcagcaaag
    10741 agtacgatga aagcctgggc acggacgatg tgcattatca gtcgttcgcc cgcatggcgg
    10801 cattttttgg ccgccatatg ctgccacatc gccacgaaca gtactttcag atgcatttcc
    10861 tcaatagcgg acagattgag ctacagcttg acgatcatcg ctactcggtg gaagcgcccc
    10921 tgtttgtcct gacgccgccg tcagtacctc atgcgtttat tacggagtct gacgccgacg
    10981 gtcatgtgtt gacggtacgg gaagatctga tctggcccct gctggaagtt ctttatccgg
    11041 gcactcggga aaccttcggc ctgccggggg cctgtcactg gcagataaac ccgacgaact
    11101 ggcggcgctg gaacactatt ggcaactgat agagcgggaa tcggtggaac aactgcctgg
    11161 acgggaacac accctgacgt tactggcaca ggcagtgttc accctactgc tgcgtaacgc
    11221 aaaactcgac gaccatgccg ccagcgggat gcgcggagaa ttaaaactgt tccagcgttt
    11281 taatatgctg attgaaagcc attttcatca gccctggaca gtaccggatt acgctaacga
    11341 actgcatatc accgaatcac gactcacgga catctgccgt cgctttgcca accgtccgcc
    11401 aaaacggttg attttcgaca ggcagctgcg agaagccaag cggctgctgc tgttttctga
    11461 taacgccgtg aacaatattg cctggcaact cggttttaag gatccggctt attttgcgcg
    11521 cttttttaat cgcttagtcg gttgctcgcc cagtgcttat cgcgccaaaa aagtacctgt
    11581 gacgtgaaaa tcccttagcc ttaacgggaa accaggcacc acctgctatt cccctttctc
    11641 cgcctggagc aaggggattt agtaacgtca aaaatcgcta aaagcgacct cgatcacaaa
    11701 accgtacccg acctgaaaag taccagccat tgtcccaaaa gtctcttccc gtaatcgccc
    11761 ctgcaagctt taattgaata acaaaaacaa cacacaaaat taacaatcgt attccgatta
    11821 atactgtaga ggtcgacatg aaaccagaag atttccgcgc cagtacccaa cgtccgttca
    11881 ccggggaaga gtatctgaaa agcctgcagg atggtcgcga gatctatatc tatggcgagc
    11941 gagtgaaaga cgtcactact catccggcat ttcgtaatgc ggcagcgtct gttgcccaac
    12001 tgtacgacgc gctacacaaa ccggagatgc aggactctct gtgctggaac accgacaccg
    12061 gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa aagtgccaac gacctgcgcc
    12121 agcaacgcga tgccatcgct gagtggtcac gcctgagcta tggctggatg ggccgtaccc
    12181 cagactacaa agctgctttc ggttgcgcac tgggcgcgaa tccgggcttt tacggtcagt
    12241 tcgagcagaa cgcccgtaac tggtacaccc gtattcagga aactggcctc tactttaacc
    12301 acgcgattgt taacccaccg atcgatcgtc atttgccgac cgataaagta aaagacgttt
    12361 acatcaagct ggaaaaagag actgacgccg ggattatcgt cagcggtgcg aaagtggttg
    12421 ccaccaactc ggcgctgact cactacaaca tgattggctt cggctcggca caagtgatgg
    12481 gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat ggatgccgat ggcgtaaaat
    12541 taatctctcg cgcctcttat gagatggtcg cgggtgctac cggctcgcca tacgactacc
    12601 cgctctccag ccgcttcgat gagaacgatg cgattctggt gatggataac gtgctgatcc
    12661 catgggaaaa cgtactgatc taccgcgatt tcgatcgctg ccgtcgctgg acaatggaag
    12721 gcggttttgc ccgtatgtat ccgctgcaag cctgtgtgcg cctggcagtg aaattagact
    12781 tcattacagc actgctgaaa aaatcactcg aatgtaccgg caccctggag ttccgtggtg
    12841 tacaggccga tctcggtgaa gtggtggcgt ggcgcaacac cttctgggca ttgagtgact
    12901 cgatgtgttc tgaagcgacg ccgtgggtca acggggctta tttaccggat catgccgcac
    12961 tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa gatcaaaaac attatcgaac
    13021 gcaacgttac cagtggcctg atctatctcc cttccagtgc ccgtgacctg aacaatccgc
    13081 agatcgacca gtatctggcg aagtatgtgc gcggttcgaa tggtatggat cacgtccagc
    13141 acatcaagat cctcaaactg atgtgggacg ccattggcag cgagtttggt ggtcgccacg
    13201 aactgtatga aatcaactac tccggtagcc aggatgagat tcgcctgcag tgtctgcgcc
    13261 aggcacaaag ctccggcaat atggacaaga tgatggcgat ggttgatcgc tgcctgtcgg
    13321 aatacgacca gaacggctgg actgtgccgc acttgcacaa caacgacgat atcaacatgc
    13381 tggataagct gctgaaataa cgcagcagga ggttaagatg caattagatg aacagcgcct
    13441 gcgctttcgt gacgcgatgg ccagcctgtc ggcagcggta aatattatca ccaccgaggg
    13501 cgacgccgga caatgcggga ttacggcaac ggccgtctgc tcggtcacgg atacaccacc
    13561 atcgctgatg gtgtgcatta acgccaacag tgcgatgaac ccggtttttc agggcaacgg
    13621 taagttgtgc gtcaacgtcc tcaaccatga gcaggaactg atggcacgcc acttcgcggg
    13681 catgacaggc atggcgatgg aagagcgttt tagcctctca tgctggcaaa aaggtccgct
    13741 ggcgcagccg gtgctgaaag gttcgctggc cagtcttgaa ggtgagatcc gcgatgtgca
    13801 ggcaattggc acacatctgg tgtatctggt ggagattaaa aacatcatcc tcagtgcaga
    13861 aggtcacgga cttatctact ttaaacgccg tttccatcca gtgatgctgg aaatggaagc
    13921 tgcgatttaa gctccagccc cttcacgtat tcgtcgaagg ggcattttta ttgtgatctc
    13981 aatcacatat ccacccgatg aataattctt gcccaaccgc tcaccgccat tcctgaccgc
    14041 ttacgtccct aaaataacca ctcagttatt taccttactt tacgcgcgcg taactctggc
    14101 aacatcactg caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa cctagaacgg
    14161 cttcggccta tgattaacaa attactctat gaattttggt ttgcaggaag gcagcaagtg
    14221 agtgaatccc cgggagctta caacagtaag tgacagaggt gaacgaacgc agctgccgca
    14281 cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta ctatggatac gaaaaaacta
    14341 ttcaagcaca taccctgggt gattctcgga atcatcggtg cattctgcct cgcggtagtt
    14401 gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc tgtgtcggtg
    14461 tatctggtgg cgtatcgcta ctacagtctg tacatcgccc agaaggtgat gaaactcgac
    14521 cccacgcgcg cgacgcctgc ggttattaac aacgacggtc tgaactacgt tccgaccaac
    14581 cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg gtgccggtcc gctggttggt
    14641 ccggtgctcg ccgcgcagat gggctacctg cctggcacgc tgtggctgct ggcgggggta
    14701 gtactggccg gtgcggttca ggactttatg gtgctgttta tctcctctcg ccgtaacggc
    14761 gcatctctcg gtgagatgat caaagaagag atgggaccag taccggggac tatcgcgctg
    14821 tttggctgtt tcttaatcat gatcatcatc ctcgccgttc tggcgctgat tgtggttaaa
    14881 gccctggccg aaagtccgtg gggtgtcttc accgtttgct caaccgtacc gattgcgctg
    14941 tttatgggta tctacatgcg ctttatccgt ccggggcgtg taggtgaagt ttctgttatt
    15001 ggtatcgtgc tgctggttgc ctctatctat ttcggtggcg tgattgctca cgatccgtac
    15061 tggggtccgg cactgacctt taaagacacc accattacct tcgcgctgat tggctatgcg
    15121 tttgtttccg cactgctgcc ggtgtggctg atcctcgcac cgcgcgacta tctggcaacc
    15181 ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta tcgtggtact gaacccggaa
    15241 ctgaaaatgc ctgcaatgac ccagtacatt gacggtactg gcccgctgtg gaaaggcgct
    15301 ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg tatctggctt ccacgcgctg
    15361 atctcttccg gtacgacgcc gaaattgctg gctaacgaaa ccgacgcgcg tttcattggc
    15421 tacggcgcaa tgctgatgga atccttcgtg gcgattatgg cgctggttgc tgcgtccatc
    15481 atcgaaccgg gtctttactt cgcgatgaat accccgcctg ctggccttgg catcaccatg
    15541 cctaacctgc atgaaatggg tggcgagaac gcgccgatca tcatggcgca gctgaaagac
    15601 gttaccgcac acgcggcggc gaccgtcagc tcctggggct tcgtgatttc gccagagcag
    15661 atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc aggtggcgcg
    15721 ccgacgctgg cggtaggtat cgctcacgtg ttccacaaag tgctgccgat ggctgacatg
    15781 ggcttctggt atcacttcgg tattctgttc gaagccctgt tcatcctgac cgcgctggat
    15841 gcgggtactc gttctggccg ctttatgctg caagacctgc tgggtaactt catcccgttc
    15901 ctgaaaaaaa ccgactctct ggttgccggt atcatcggta ctgcgggctg tgtgggtctg
    15961 tggggctacc tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa gagcctgtgg
    16021 ccgctgttcg gtatttccaa ccagatgctg gcagccgtag cgctggtact gggcaccgtt
    16081 gtgctgatta agatgaagca cacccaatac atctgggtaa ctgttgttcc ggctgtatgg
    16141 ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt tcagcaccaa cccgcagatg
    16201 gaaggcttct tctacatggc aagccagtac aaagagaaga ttgctaacgg tactgacctg
    16261 acggcgcagc agattgccaa catgaaccac atcgttgtga acaactacac caacgcaggc
    16321 ctgagtattc tgttcctgat tgtggtgtac agcatcatct tctacggttt caaaacctgg
    16381 cttgcggtgc gtaacagcga caaacgtact gacaaagaaa ctccgtacgt tccaatcccg
    16441 gaaggcggcg tgaagatctc ttcgcaccac taaccggttg ccggatgcgc tgcgcgtatc
    16501 cggcctacaa agtgtctatc gtaggccgga taaggcgttt acgccgcatc cggcaaatcc
    16561 ctagcccgga ccttccgggc tttacgcata tctggaaggc aaaatgtttg gtaacttagg
    16621 acaggcaaaa aaatatctcg gccaggcggc gaagatgttg attggcattc cagactacga
    16681 caactacgtc gagcatatga agaccaacca tcccgacaag ccgtacatga gctatgaaga
    16741 attcttccgc gaacgccaga acgcgcgcta cggcggcgat ggtaaaggcg gtatgcgctg
    16801 ttgttaatgg agagagcatg aacccgattg cagttaccct actcaccggt tttttaggcg
    16861 cgggcaaaac caccctgctg cgccatattc ttaacgaaca acacggctac aagattgccg
    16921 tgattgaaaa cgaattcggc gaagtctccg ttgatgatca actgattggc gaccgcgcca
    16981 cgcagatcaa aacgctgacc aacggctgca tctgctgttc gcgctccaac gagctggaag
    17041 acgcactact cgacctgctg gataatctcg acaagggcaa tattcagttc gaccgtctgg
    17101 tcattgaatg caccggcatg gccgatcccg gcccgattat tcagaccttc ttctcccatg
    17161 agattttatg ccagcgctac ctgctggacg gggtgattgc gctggtggat gcggtacatg
    17221 ccgatgagca gatgaaccag ttcaccatcg cccagtccca ggttggctac gccgaccgta
    17281 ttctgctgac gaaaaccgac gtcgcaggcg aagcagagaa actgcgtgaa cgcctggcgc
    17341 gcatcaacgc ccgcgcaccg gtctacaccg ttacccacgg cgatatcgac ctgggccttc
    17401 tgttcaacac caacggtttt atgctggaag aaaacgtcgt cagcaccaaa ccgcgtttcc
    17461 actttatcgc ggacaaacaa aacgatattt cgtcgattgt ggtggaactg gattacccgg
    17521 tagatatcag cgaagtttcc cgggtgatgg aaaacctgct gctggaatcg gcggataaac
    17581 tgctgcgtta caaagggatg ctgtggattg acggcgaacc taaccgcctg ctgttccagg
    17641 gcgtccagcg cctctacagc gccgactggg accgcccgtg gggcgatgaa aaaccgcata
    17701 gcacgatggt gtttatcggg attcagttgc cggaggatga gattcgggcg gcgtttgcgg
    17761 gactcaaaaa gtaaccagcc ctccaacatc acaatacctg agactttatc accctcatat
    17821 tcctccagaa tatgagggca ttcgggctta attatctctg aacgtgcaac acttcctacg
    17881 actccttatg cgcctccaca tcctgcttcg ttaaccagag cgacttacga ccaaaatttt
    17941 cgtacatttt cagctgatct tcatagtgct gatcaactgt tccatcggga gcaataaacc
    18001 cactctgacc gggtgcgacc acatcccagg caagcaccgg acgatcgctt gtcgttggtg
    18061 agaaaacaat catatcgttt tctgttccac ggttttgata ctccgcctga tgacgcgttt
    18121 cttccgctgc ggcctgcggt acatcaaaga aattatttgc ccggaacgtt aaggccattg
    18181 caggtgtttt ccagttactc acattattgc catagcgttt ggaaagagtc tcccaggtat
    18241 cttccagcgc agccaacaca acctcctgct gtggtttccc agcaaacaga tcaaccgcct
    18301 gtgggattgg tgatttgtct ccctgcaccg cctcatacaa aatttttgct ccaacactta
    18361 tattcagcga accagttggg ccgtcctggg ttgtttcgta gccactggcg ctgtaccact
    18421 tatcaaatgg cataggtacg gcagccacta cggtacgctt caacatactg gtcagccaaa
    18481 cgttcaggat ggcagagcct ggc
//
LOCUS       DADLTD010000072        17926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLTD010000072 DADLTD010000000
VERSION     DADLTD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.072"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..118)
                     /locus_tag="JN701_001932"
     CDS             complement(<1..118)
                     /locus_tag="JN701_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY7364393.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMV"
     gene            679..1044
                     /locus_tag="JN701_001933"
     CDS             679..1044
                     /locus_tag="JN701_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7364394.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1084..1779
                     /locus_tag="JN701_001934"
     CDS             1084..1779
                     /locus_tag="JN701_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAY7364395.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1846..3264
                     /locus_tag="JN701_001935"
     CDS             1846..3264
                     /locus_tag="JN701_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY7364396.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3245..3715
                     /locus_tag="JN701_001936"
     CDS             3245..3715
                     /locus_tag="JN701_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY7364397.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3704..4624)
                     /gene="yedA"
                     /locus_tag="JN701_001937"
     CDS             complement(3704..4624)
                     /gene="yedA"
                     /locus_tag="JN701_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY7364398.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4797..5714
                     /locus_tag="JN701_001938"
     CDS             4797..5714
                     /locus_tag="JN701_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY7364399.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5793..5975
                     /locus_tag="JN701_001939"
     CDS             5793..5975
                     /locus_tag="JN701_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY7364400.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6146..7840
                     /gene="yedQ"
                     /locus_tag="JN701_001940"
                     /pseudo
     CDS             6146..7840
                     /gene="yedQ"
                     /locus_tag="JN701_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(7837..8652)
                     /locus_tag="JN701_001941"
     CDS             complement(7837..8652)
                     /locus_tag="JN701_001941"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HAY7364401.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGVPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8950..9166)
                     /gene="yodD"
                     /locus_tag="JN701_001942"
                     /pseudo
     CDS             complement(8950..9166)
                     /gene="yodD"
                     /locus_tag="JN701_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9275..9517
                     /gene="dsrB"
                     /locus_tag="JN701_001943"
     CDS             9275..9517
                     /gene="dsrB"
                     /locus_tag="JN701_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY7364402.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            complement(9561..10184)
                     /gene="rcsA"
                     /locus_tag="JN701_001944"
     CDS             complement(9561..10184)
                     /gene="rcsA"
                     /locus_tag="JN701_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY7364403.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10469..11254)
                     /gene="fliR"
                     /locus_tag="JN701_001945"
     CDS             complement(10469..11254)
                     /gene="fliR"
                     /locus_tag="JN701_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAY7364404.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11263..11532)
                     /gene="fliQ"
                     /locus_tag="JN701_001946"
     CDS             complement(11263..11532)
                     /gene="fliQ"
                     /locus_tag="JN701_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY7364405.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11542..12278)
                     /gene="fliP"
                     /locus_tag="JN701_001947"
                     /pseudo
     CDS             complement(11542..12278)
                     /gene="fliP"
                     /locus_tag="JN701_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(12278..12643)
                     /gene="fliO"
                     /locus_tag="JN701_001948"
     CDS             complement(12278..12643)
                     /gene="fliO"
                     /locus_tag="JN701_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY7364406.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12646..13059)
                     /gene="fliN"
                     /locus_tag="JN701_001949"
     CDS             complement(12646..13059)
                     /gene="fliN"
                     /locus_tag="JN701_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY7364407.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(13056..14060)
                     /gene="fliM"
                     /locus_tag="JN701_001950"
     CDS             complement(13056..14060)
                     /gene="fliM"
                     /locus_tag="JN701_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY7364408.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14065..14529)
                     /gene="fliL"
                     /locus_tag="JN701_001951"
     CDS             complement(14065..14529)
                     /gene="fliL"
                     /locus_tag="JN701_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY7364409.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14634..15761)
                     /gene="fliK"
                     /locus_tag="JN701_001952"
     CDS             complement(14634..15761)
                     /gene="fliK"
                     /locus_tag="JN701_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY7364410.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15758..16201)
                     /gene="fliJ"
                     /locus_tag="JN701_001953"
     CDS             complement(15758..16201)
                     /gene="fliJ"
                     /locus_tag="JN701_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY7364411.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16220..17588)
                     /gene="fliI"
                     /locus_tag="JN701_001954"
                     /pseudo
     CDS             complement(16220..17588)
                     /gene="fliI"
                     /locus_tag="JN701_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            complement(17588..>17926)
                     /gene="fliH"
                     /locus_tag="JN701_001955"
     CDS             complement(17588..>17926)
                     /gene="fliH"
                     /locus_tag="JN701_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY7364412.1"
                     /translation="ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
BASE COUNT         4398 a         4488 c         4716 g         4324 t
ORIGIN      
        1 caaccatctt gccgtagaaa tcaactttat tgccatcttt attgtaaatt tcagccgcat
       61 ttgctgcgcc agcaactaat aacgccggga ccagcattgc cagaactttt cttttcatta
      121 tgtattccct tgtgattata atcttcatga atatatcaat aagtgccgtt atccaaaaaa
      181 aagcacattt ggatactatt ctatgaagtt catttcattt taagtattac atgcaacaaa
      241 tgtatttaaa agatttcaaa atgtttctaa tctatttaat ataattcaga gatatgaatt
      301 ataaatattc atgaaaatta taaatgtata tcaatatgtt atgagttgag cggaaacata
      361 gggggtatca atgattgttg cgccaaacgg ctggtgtgat caggcgcaca ttaatgaagg
      421 cataaaaaaa agccggcatc atgccggccc aaaaaatata aacaagacga taaaaggcta
      481 ataacggaag catcatgaca cagaataata aaaaggtata attgtaatca gtgctaatat
      541 attgattaaa tgaattaata aagccgatat caaagataaa taaaatcgct caaaacgatt
      601 tttcctgtat tcactgccgt tgcgcaaaat ttatctattt gttcaaaaaa tgattgagtc
      661 ttgactggct catccaatgt ggaaaaatgt gacttttatc acataatagt actaagtctg
      721 aattttccgg gttatctcaa aatggaatac ggttcgacaa agatggaaga gagactctct
      781 cgcagccctg gcggcaaact ggctttgtgg gctttctata catggtgtgg ctatttcgtc
      841 tgggcaatgg cgcgttacat ctgggtgatg agccggatcc ccgatgcccc cgtgtcggga
      901 tttgaaagcg atttaggctc taccgcggga aagtggttag gggcactggt cggattttta
      961 tttatggctc tggtcggggc attgttaggc agcattgcct ggtacacccg acctcgtccc
     1021 gcacgtagca gacgctatga ataaaaactg ctaaggtggc gactctttgg caacggagcg
     1081 cgtatggact tacaacactg gcaggcacag ttcgaaaact ggttgaaaaa ccatcatcag
     1141 caccaggacg cggcccatga cgtgtgtcat tttcgccgcg tctgggctac ggcacagaag
     1201 ctggcggcag atgacgatgt cgatatgctg gtgattttaa ccgcctgtta ttttcacgat
     1261 attgtgagcc tggcgaaaaa tcatccgcaa aggcagcgtt cttcaatcct ggcggcagaa
     1321 gagacgcgtc gcctgctccg tgaagagttc gagcaatttc cggcggagaa aatcgaggcc
     1381 gtttgtcatg tcattgccgc tcacagtttc agcgcgcaaa tcgccccctt aacaacggaa
     1441 gctaaaatcg tccaggatgc ggatcggctg gaagccctgg gggcgattgg cctggcgcgc
     1501 gtgtttgccg tttcaggggc attgggcgtg gcgctgtttg atggtgaaga tccgttcgca
     1561 cagcatcgcc cgcttgatga taaacgctac gcgctggatc atttccagac taagttgctg
     1621 aagctaccgc aaaccatgca aaccgcaaga ggcaagcagc tggcgcagca caacgcgcat
     1681 tttttagtcg agtttatggc gaagctcagt gccgaactgg cgggggagaa tgaaggtgtc
     1741 gatcacaagg tgatagatgc gttttcaccc gcgggataag cgcgtggccc taaatggctg
     1801 taattatgtt aacctgtcgg ccatctcaga tggccggtta aatctatgca ggaaaatata
     1861 tcagtaaccg attcatacag caccgggaat gccgcacagg caatgctgga gaaactgctg
     1921 caaatttatg atgttaaaac gctggtggcg cagcttaatg gtgtgggtga gaatcactgg
     1981 agcgcggcaa ttttaaaacg tgcgctggcg aatgactcgg catggcaccg tttaagtgag
     2041 aaagagttcg cccatctgca aacgttgtta cccaaaccac cggcacatca tccgcattat
     2101 gcgtttcgct ttatcgatct atttgccgga attggcggca tccgtcgcgg ttttgaatcg
     2161 attggcggac aatgcgtgtt taccagcgaa tggaacaaac atgcggtacg cacttataaa
     2221 gccaaccatt attgcgatcc ggcgacgcat cattttaatg aagatatccg cgatatcacc
     2281 ctcagccata aagaaggcgt gagtgatgag gcggcggcgg aacatattcg tcaacacatt
     2341 cctgaacacg atgttttact ggccggtttc ccttgtcagc cattttcgct ggctggcgta
     2401 tcgaaaaaga actcgctcgg gcgggcgcac ggttttgcct gcgataccca gggcacgctg
     2461 ttttttgatg tggtacgcat tatcgacgcg cgtcgtccgg cgatgtttgt gctcgaaaac
     2521 gtcaaaaacc tgaaaagtca cgaccagggt aaaacgttcc gcatcatcat gcagacgctg
     2581 gacgaactgg gctatgacgt ggctgatgca gaagataacg ggccggacga tccgaaaatc
     2641 atcgatggta aacattttct gccgcagcac cgtgaacgca tcgtgctggt gggttttcgt
     2701 cgcgatctta atctgaaagc cgattttact ctgcgtgata tcagcgaatg tttccctgca
     2761 cagcgagtga cgctggcgca gctgttggac ccgatggtcg aggcgaaata tatcctgacg
     2821 ccggtgctgt ggaagtacct ctatcgatat gcgaaaaaac atcaggcgcg cggtaacggc
     2881 ttcggttatg gaatggttta tccgaacaat ccgcaaagcg tcacgcgtac gctgtctgcg
     2941 cgttattaca aagatggcgc ggaaatttta atcgatcgcg gctgggatat ggccaaaggt
     3001 gagaaagact ttgacgatcc gctgaatcag caacatcgtc cacgtcggtt aacgcctcgg
     3061 gaatgcgcgc gcttaatggg ttttgaagcg ccgggagaag cgaaattccg cattccggtt
     3121 tcggacactc aggcctatcg ccagttcggt aactcggtgg tcgtgccggt ctttgccgcg
     3181 gtggcaaaac tgcttgagcc aaaaatcaaa caggcggtgg cgttgcgtca gcaagaggca
     3241 caacatggcc gacgttcacg ataaggccac tcgcagcaaa aatatgcgcg cgattgccac
     3301 gcgtgatacg gcgattgaga agcgcctcgc cagtctgtta accgggcagg gcctggcgtt
     3361 tcgcgttcag gacgccagtc tgcccggaag cccggatttt gtcgttgatg aatatcgctg
     3421 cgtgatattt acccatggct gcttctggca tcatcatcac tgctatctgt ttaaagtgcc
     3481 tgcgactcga accgagttct ggctggagaa gataggtaaa aatgttgaac gcgatcgccg
     3541 cgatatcagt cgtttgcagg aactcggctg gcgcgtactg attgtctggg agtgtgcatt
     3601 acgtgggcgc gagaagctga cggatgaagc gcttaccgag cgtctggaag agtggatctg
     3661 cggcgaaggt gccagcgcgc agatcgacac gcaggggatt catttactcg cttgatgcgt
     3721 cctgaataac tggcgcaact acgggttttg ccgggaagag atattttccc aacgtgacca
     3781 gtaccaccgc gaagacaatt acgccgagcg ccagccattc aatcttcgac agcgtctctc
     3841 cgcccagtcc cgtacccagc aagaccgcga ccaccgggtt aacgtaggcg tagctggtgg
     3901 cgagagccgg actgacatta cggattaaat acatataagc gttgatggcg ataatcgaac
     3961 caaacagcgc cagatagccg accgcaagga agcctgaaag ggaagggagc gccgtcagtt
     4021 tttcacctgc aatcatcgac gcgatcatta acaccacgcc tgccgccagc atctcaatcg
     4081 cacccgccat catccctaca ggtaaggtaa tgcgcgagcc ataaaccgag ccaaacgccc
     4141 agctaatcga gccgattaaa atcagaatcg cgccccacgg attgccgctt aaatttccac
     4201 cgctattgag catgatgatt ccggcaagcc caatggcgat acccacccat tccaatttgc
     4261 gcgttttaat gccaaacagg cggctgaagc acagggtaaa gaggggcacg gttgcaacca
     4321 caacggcggc gatgccggaa ggaacatttt gatgctcggc aaccgtcacc atgccattac
     4381 cgacagccag caataacagg ccaatcagcg cggcattgag cagcggacgt agcgggggga
     4441 gcttgtgtcc gcgcagtagc aaaaatgcca tcaataaaat accggctgcc aggaatcgaa
     4501 cgcctgccat cattaacgga ggccagcttt ccacgccaat ccgaatgaca aaataggttg
     4561 agccccaaat gatatacaac gcaaacagcg cgccaaaaag cggtaacaac tggcggaaac
     4621 gcataatccc tcacggtgga aataaaaagg tggttcatag taaacgtgaa aatcattctg
     4681 ctggcgagag atataattgc acttgattgt taaaaaaatg ttgacctgtg aagcaagtat
     4741 cagagctgcc gtttttgctt catacttaca cccttcaaca ataaaaatga gagggaatgc
     4801 ttttggccgg gagtagttta ctgacgttgc tcgatgatat cgccacacta ctggacgata
     4861 tctccgtgat gggcaaactg gcggcgaaga aaaccgccgg tgtattaggg gatgacttat
     4921 cgctcaatgc gcaacaagtt tcaggcgtgc gggccaaccg ggaacttccc gtggtctggg
     4981 gcgtggcgaa aggatcgctg attaataaag tgattctggt gccgctggcg ctgatcatca
     5041 gtgcgtttat cccgtgggcg attacgcctc tgttgatgat tggtggcgcg tttctctgct
     5101 ttgaaggagt agagaaagtg ctgcatatgc tggaggcgcg taaacataaa gaagatccgg
     5161 cgcagagcca gcagcgtctg gagaagctgg cggcgcagga tccgctgaag tttgaaaagg
     5221 acaaaataaa aggggcgatt cgtaccgatt ttatattgtc tgccgaaatc gtcgccatca
     5281 cgctggggat tgtggccgaa gcgccgttgc ttaatcaggt gctggtgctt tcaggcatcg
     5341 cgctggtagt gaccgtgggc gtctacggtc tggtaggggt tatcgttaag attgatgacc
     5401 tggggtattg gctggcggaa aaatccagcg cgctgatgca ggcattaggt aaagggttat
     5461 tgatcattgc gccctggctg atgaaagcgt tatcgattgt cggcacgctg gcgatgttcc
     5521 tcgtcggcgg cgggattgtg gtacatggta ttgcgccgct gcatcacgcc attgaacatt
     5581 tcgccgggca gcaaagtgca gtggtggcga tgatattacc gactgtttta aatctgattc
     5641 ttggatttat catcggcggc atcgtggtgc tgggagtgaa agccgtagcg aaaatacgcg
     5701 gtcaggcaca ttaatactcg cacaggctat tatgcaaaat tcgccatctt caactaaggt
     5761 gaacaagttt cactcgaaaa aaggaggcga gtatgagctt tatggttagt gaggaagtta
     5821 cggttaaaga gggcggcccg cggatgattg tcaccggata ctccagcggt atggttgagt
     5881 gtcgttggta tgacggttac ggggtcaagc gggaagcttt tcatgaaacc gagcttgttc
     5941 cgggggaggg gagtcgttct gcggaagaag tttgaaaggg tatcattaac accaggccgg
     6001 atcacttccg gcctggtgtg ccaatgcctg caatgaatga tagtcgcgtc ttatcatgcc
     6061 accattataa aattatcgct gttttgcggt acgctatcgc caggttatgc cagaatcata
     6121 aaaaagcagg ttgggagtcg tcagggtgca gcacgagaca aaaatggaaa accagagctg
     6181 gttgaaaaaa atcgcacgcc gcctggggcc tggtcatgtc gttaatctct gctttatcgt
     6241 ggtattgctt ttttccacct tgctgacctg gcgtgaagtg gtagtgctgg aagatgccta
     6301 tatctccagt cagcgtaatc atctggaaaa cgtagccaac gcgctcgata agcatttgca
     6361 gtataacgtc gacaaactga tctttttgcg taatggcatg cgcgaagctc tcgtagcgcc
     6421 actggatttc acttcactgc gtaatgctgt aaccgagttc gaacagcatc gcgacgagca
     6481 cgcctggcaa attgaactca accgacgacg caccctgtca gtcaatggcg tatcggatgc
     6541 attagtcagc gaggggaatc tcctgtctcg cgaaaatgaa agcctcgaca atgaaattac
     6601 cgctgcactg gaagttggtt acttgctgcg actggcgcac aacacctcgt cgatggttga
     6661 acaggcgatg tatgtctcgc gtgccggatt ttacgtttcg acgcagccga ccttgtttac
     6721 gcgcaatgta ccaacgcgtt attacggcta tgtcacccaa ccctggttta tcggccattc
     6781 gcaatgagaa aatcgtcacc gcgcggtacg ctggtttact tcgcaaccgg aacacgccag
     6841 caatactgaa ccgcaggtta ccgtcagtgt tccggtagac agtaataact actggtatgg
     6901 cgtgctgggg atgagtattc ccgtgcgtac catgcagcaa tttttaagaa acgccatcga
     6961 taaaaacctc gatggtgagt atcagctcta tgacagtaag ctgagatttt tgacctcttc
     7021 caatcctgac catccaacag ggaatatttt tgatcctcgt gaactggcct tgctggcgca
     7081 ggcgatggaa catgacacgc ggggcggcat tcgtatggac agtcgctatg ttagctggga
     7141 acgtctggac catttcgacg gtgtgctggt gcgcgtccat acgctaagcg aaggcgtgcg
     7201 cggcgatttc ggcagtatca gcattgcatt aaccctgctg tgggcgctct ttaccaccat
     7261 gttactcatc tcctggtatg tgattcgccg gatggttagc aacatgtatg ttctgcaaag
     7321 ctcgttgcag tggcaggcgt ggcacgacac cttaacccgt ttatataatc gtggcgcact
     7381 gtttgaaaaa gctcgtccgc tcgcgaaatt gtgtcagacg caccaacatc ctttttctgt
     7441 cattcaggtc gaccttgacc attttaaagc gattaatgac cgctttagtc atcaggcggg
     7501 cgaccgtgtt ctttctcatg ctgccggatt aattagcagt tccttgcgtg cgcaggacgt
     7561 tgccgggcgg gtcggtggtg aggagttttg tgtgattctg ccaggcgcga gtctgacgga
     7621 ggctgcggaa gtcgcagaac gtattcgcct gaagttaaat gaaaaagaga tgttgattgc
     7681 taagagtacg acgatacgca tcagtgcctc gttgggggta agtagcagcg aggaagccgg
     7741 tgattatgat tttgaacaac tccagtcact ggctgaccgt cggctttatc tcgctaaaca
     7801 ggccgggcgt aatcgggtat gcgcgagcga taacgcttaa cgagcggaga aaaaatggtc
     7861 cagcccttca cgccatcctt ccggtccttc acgctgcgtt cgccagacgc gggccggatc
     7921 ctcatcatgc agatgcaccc cttcacggtt tagccctttc acaatcaccg cgtaatccat
     7981 tacctccagt aagggcgcat cgtttggccc atcgcccagg ccaagtgtgg ttgggcgttt
     8041 gcctgataat tgttgatagg tcgcgataat ccagttggcg gcctgatctt ttccggcaga
     8101 ggcatccagg acgtgccaga agcgcgcacc ctgcataaac tgtaagccca gttcgttcag
     8161 acgagcggta aattgtgcca tacgctcgtc actgtcgcgc cagattagcg ttaccgacgc
     8221 ctcatgcagc tgcgtcagcg ccgcctggct acggcttaat cccgtccatt cggcgatggt
     8281 tgcatcgtcg acatcatcga aagtcgtgaa tttaaaatgt tctttctcgc gtagcgtatt
     8341 taaaacctgg ctgatttcgc catggctaat acctgagatg atgcgtggaa aaccgtctat
     8401 atcctgccat tgttcagcaa gctggatcac tgcgccattt tcggcaatca gtggtacccc
     8461 ttgtaacccc aacgtttttt gcaagtacag catttccgct gaagttttac tgctacagag
     8521 aatgacggga acattcgctt cgcgtaaacg gctgagccag ggggcagccg gttgccagtc
     8581 ataactatga ctgtccagca gggtgccatc aagatcgcta aaaaccagta gtggttgttg
     8641 aattgaaaac atggtcgcgc agttctcccc ttaccaggat gattctcaaa acagcacaaa
     8701 taatcaaaga tgattttttc gggagcgatc ccgttgacgc cagagccacc aaggggtgac
     8761 gcacgtcaca tttctattca taagtagcgt taatcattca tatcgcgaat attttcttgt
     8821 cagcgaaaaa aattgcggat aaggtgatga acacatcaga tttcctggtg taacgaattt
     8881 tttaagtgct tcttgcttaa gcaagtttca tcccgacccc ctcagggtcg ggattttttt
     8941 attgtgcatt caacgattca cttcagagac gctgaagtca tgaatatcca gttcgaaggc
     9001 atccgccaat tcacgccatg tatgatattc acgtccatcg acgctaacgt gttcagaatc
     9061 gttctggctg cgatgaactt atttcattga tcgccgccag cagggcatcg acatcgacgc
     9121 tgacctcacg ttttgcggta tcgctgtact cttttgcggt tttcatcatc tttcctcgca
     9181 accgttactc tgttgttgcc gccagtcgtt aagtccgcct ggcatctctt aagtatagac
     9241 cgtcgagaaa atcagcactc gcgcgcgcct ggcgctgcca gaaaaacgaa attgttctac
     9301 actggcacaa agccacagga ggaaaacgat gaaggtgaat gatcgggtaa cagtcaaaac
     9361 ggatggcggt ccgcgtcgtc ctggcgtggt actagcagtt gaggagttta gtgaaggcac
     9421 aatgtacctg gtttcgctgg aagactaccc gctcggcatc tggttcttta atgaagcagg
     9481 gcatcaggac ggtatctttg tggagaaagc agagtaatct tattcagcct gactggtggg
     9541 aaaccaccag tcagaatgtg ttagcgcatg ttgacaaaaa taccattagt cacattatcc
     9601 gtcagtcgga cgacatggta gataacctgt ttattatgcg ttttgatctt acgtttaata
     9661 ttacctttat gcgatgaaac ggtcttggct ttgatattca tttggtcaga gatttgaatg
     9721 gttccctgac ctgccatcca cattcgcaac atactcgatt cggttcggct caatgataac
     9781 gtcggcatat ttaaaaaaga ggttatcgtt gtctcttttt tcagaatatc gccaaggata
     9841 tcgtcgagag attccggttt aatcgattta gaacttatca ataaattttt tctgaccaat
     9901 agatattcat caaaatgaac attggcaatt gccataaaaa cgataaataa cgtattggga
     9961 tgttgattaa tgatgtgctt gatgtgctga ctgttagaag catcatggat gaaacagtcc
    10021 tcattaataa acaccactga agggcgctgt gaatcacaag ctatggcaag gtcatcaacg
    10081 gtttcaatgt cgttgatttc tcttttttta acccctctac tcaacagata cccggttaaa
    10141 cctagtcggg tgtaactaca taaatccata ataatcgttg acatggcata ccctcactca
    10201 atgcgtaacg ataattcccc ttacctgaat atttcatcat gactaaacgg aacaacatgg
    10261 gtcacctaat gcgccactct cgcgattttt caggcggact tactatcccg taaagtgttg
    10321 tataatttgc ctggaattgt cttaaagtaa atgttgcgat atgtgagtga gcttaaaaca
    10381 aatatttcgc cgcaggagta tcctggaaga tgttcgtagg tgttattttt cggataatcc
    10441 ttaggataac atgataaacg ttacggaatt atattaatgg caattcacta ataatatcag
    10501 ccagcaaatt aaacatctca ctgaataaat gttcgcaaaa aggtgcaatt aacggcatta
    10561 atgccgccat taaagagata ccgacagtca gagttaatgg aaatccaata acaaaaatgg
    10621 ataattgcgg ggccatacga ttcagtaaac ctaatgccag attcagggtc agcagcagag
    10681 taatgagcgg taacgccagc atcagcccgt taaggaaaat caaactcccc gctttggtga
    10741 gtgccagaaa cgcattgctg ttcaacggtt cgccaccaat tggcagggtg tgaaaggtat
    10801 cgaccagcag tgaaatcaac cataaatgac cgttaaatgt caggaacagc agtaacgcca
    10861 gcatatccat gatacgcgct aaaacgggca tattgagatg gctggccgga tcgacaaacg
    10921 tcgcaaatga cagccccatt tgcagaccga taatttcgcc agcggttcgc acagcggcaa
    10981 aggcaaattg catggtaaaa ccaagcgcaa tgccgatcag gatctgctgc acggccagcc
    11041 acagagcaaa gaacgaaaaa acaggaacat cgttggcagg taatgatggg gcaatgacga
    11101 acgtgatcat cattgccagc cccagtttta cccgtttcgg cacgctgcgt tcgctcagaa
    11161 ttggtgcggt agaaatgagc gccagcacgc gcagtaacgg ccagaagtac aggcttaacc
    11221 aggaaagcca ttgatcgctt gtcacctgca tcattgtacg gcctacccga tgatatacgg
    11281 caggttagtg aacaaggtgc ggacgtaatc cagcaacaga ttgagcatcc acggtccggc
    11341 aataataatg gcaataaata cggcgatgat ttttggaata aacgacagcg tcatttcgtt
    11401 gatctgcgtg gcggcctgca aaatactgat gataaggccc gtgaccagcg ccaccagcaa
    11461 cagcggggca gccagtgcca gcgcgacttt catcgcttca gtccccatca tcatgaccga
    11521 ttcaggtgtc attttgcctc tctagctgta aaagctctgc gccagcgaac cgaccagcaa
    11581 ttgccaacca tccaccagta caaacagcat cagtttaaag ggcagagcaa tggtggcggg
    11641 gggaccatca tcatccccaa tgccatcaac acgctggcaa tcaccaggtc gataatcaaa
    11701 aaaggaatga aaatcgtgaa gcctatctga aatgcggttt tcaactcgct ggtcacgtag
    11761 gccgggcgca aaatgcgcat cggcacggct tcaggtccct gcaacgggcc ggtattcgct
    11821 agtctggcaa acaaccctaa atctgcctca cgggtctgac gcagcataaa ctcacgtagc
    11881 ggctgcgccc ctttttccag cgcctcctgc attgatattt tctcttcgct gaatggctgg
    11941 tacgcatcga cataaatttt gtcgatcacc ggtgacataa taaaaaaggt caaaaatagt
    12001 gccagcccca gcaatacctg gttaggtggc gcggagggcg ttcccagcgc gttacgcaat
    12061 aaaccaaaaa caatgatgat gcgggtgaaa ctggtcatca tcagtaaaat tgccggaata
    12121 aacgtcaacg aggtgatgaa caccagcgtc tgcaccggga gcgaccagct ttgtccaccg
    12181 ccaggcagcg gctggctggt gatacccggc agttgcgcga aggcgagggg cgtaaccagc
    12241 cagagaagga caggtgcgac agacaacaaa cgacgcatca ggatctccca ctacgcttaa
    12301 gcaaattttt catgaccgac tgaaaatcgg tctgcggtat ctcttctgtt ggtgctgaag
    12361 ggggaagttt atgcagcaga ttgatttgcc ctgcggtaac gccgagcacc agccgtgcat
    12421 cttccacatc gaccaccaca acccgttcac gcgcgcccag cgaagcactg gcgctaattt
    12481 tcagaccgtt aacgccagtg cgtttcgggg caaatcccaa ccgttttacc tgccaggcag
    12541 cagcgaggat cagggcaata atggcgatga gtgcgccgct gacctgcagc agtggcgcag
    12601 cagaaaccgg cgtggaagat tgcacagtag cgtggttatt catcactaac ggctcaggcg
    12661 atgcattcgc tcagacggag taatgatatc ggtgatccgc acgccatatt tatcggcaac
    12721 gaccaccact tcgccctggg cgattaaata accgttgatc agaatatcca gtggttcgcc
    12781 cgccagaccg tccagcgcca cgacggaccc ttgcgtcaga cgcaacagct ctttgatggt
    12841 catccgcgta cggcccagct cgacggtcag cttgaccgga atatccatta tcaggtcgat
    12901 atcctgcaac gttccgctga catcaccacc gccaaattgc tggaacaccg cgtcggcagc
    12961 gcttttgctg ctggttgatt tttgttcgct caacgcttca gcccacagat cgtccattgc
    13021 gccgttgttg tcatcggccg gattattcat gtcactcatt tgggctgttc ctcgttcaga
    13081 gaatttaaaa tcgggttaat caaatgttct atccgtaacg catactgacc gttgagagtg
    13141 ccgtactgac tggtcagcac cgggacgccg tcaacatggg cgatgatgcg atcaggtttt
    13201 tctatcggca ggacgtcgcc gggtttcagt tttaaaatct gcgacaggcg tagcgagata
    13261 tcggcaaagt tggcgaccag ctccagctgt gaatgctgca cctggcgcac caggttatcg
    13321 cgccagttct gatcttcatt acgcgagttt tccagcggcg ggttaaccaa caattcccgt
    13381 agcggctcga tcatgctgaa tggcagacag atattaaatt cgccggtcag gttgccaatc
    13441 tccacatgga acggcgtgtt aaccacaatg tcgttcggcg aggtggtgat attggtaaat
    13501 ttcacctgca tttccgaacg cacgtactca acttccagcg gattaatcgc cttccaggcg
    13561 tcgctatagc cttcaagcgc cagctttaac atgcggttga tgacgcgctg ttcggtatgg
    13621 gtaaactcgc gaccttccac tttggtcggg aagcgtccat cgccgccaaa caggttatcc
    13681 acggcgataa acaccagact cggtgaaaac accaccagcc ctgtgccgcg cagcggtttc
    13741 agatggataa ggttcaggtt ggtcggcacc ggcaggttgc gggcaaattc atggtacggc
    13801 tgaatgcgga tggccccgac ggttatatcc gggctacgac gcagcaggtt gaacagcccc
    13861 atacgaaaat ggcgggcaaa gcgctcatta atgatttcca gcgcctgcaa acgttcgcgc
    13921 acaacccgtc gttgggtatt cggatcgtac ggacgaatgt cactttcgcc gctaacactg
    13981 gctgtcggtt cgtctttgac ttcgctgtca ccattcaaca gcgcatcaat ttcagcttga
    14041 gaaagaatac tatcgcccat gtcgttatcg cagaataaaa gcggtataca gcacgtcggt
    14101 gacatcctgt ttcggttgcc cggcaacaag cggggtggaa agcgtggttt taatctcggc
    14161 aatcaggttt ttcttgcctt cttctgtcgc cagtacggca gcatcctgac gcgaaaacag
    14221 taacagcaaa cggctacgga cttccggcaa atactcactc agccgcgagc gggtagcttc
    14281 atctttcagg cgcagggtta tgccgatata aagcacgcga tccgcatcgc ccaaattgac
    14341 cgtgaaggta ttcagcgcgt agaagaccgg ggagggtacg acgcgttgct gcgctttgtc
    14401 gtcagcggca acctgatgcg aatgccagta gctgtaacct gcgctggcac aggccgcgag
    14461 ggtaatgaat accagaatcg ggatccaaag cgatcgcttg cttttcttgc ttatcgcgta
    14521 atcagtcatg tgttgcggtc ttcctgtgtc gctactgctt atcggctaat tatcctgcgt
    14581 tttgagcgca acaaagcggg gaaaagacgc ggattacggt gctacctctg acgttaggcg
    14641 aaaatatcaa cgccgctgtt gcctgttaca cgcccttgta aagagacggg aaccggaagc
    14701 gtatcgtcgt cttcccccgc cagaggttca tggtttactg tgcgttggct ttgctgttgt
    14761 tgggaagcgg cctgctgctg accactaaag ctttcgccac tgatgttgct ttgccctaac
    14821 tgaatgccac tttcggccag ctgcgtacgc agtaccggaa gcgctgcttc cagcgcggcg
    14881 cgtacatgct gatgcggtga aaccatctgg atttgcgcct ggttatcatc cactttgagg
    14941 gagatttgca cttcacctaa atcctgcggg tgcagacgca actctgcact ttgttgcccc
    15001 tggcgggtga acagcgaaat atgctggctt aatgattgtt gccattcgtg agaacccagc
    15061 ggtgcactca aaacaggcgc ggcgacggtg ggcagtggct gtgtctggtg tggagtaatt
    15121 agcgggctgg cggcagcggt caccggtgac ggtgtgctga tgacttccgc tttactctgg
    15181 gcttctgcta ccagcggtgt taatggctga gctggcgtgc cgggggcatc atcaggctgt
    15241 gctgttgtga gttgcgcaga agtcagtttt gtgaagagcg ttggtttctc tgccggtaac
    15301 acggttgacg gcacatcagt cactttgggc gtattgtcaa aacccggcaa catcgcaaaa
    15361 agggcgctca ggcttgcggt gacgtcttca ttcagatcat ccgctttttc gtcttttgtc
    15421 gtattttggt cagccaccgc agccaacgtc atcgtctgag cggtggttaa cggtgttgat
    15481 gtggattgtt cgtcgttgat gacaggcagt gtttcatcca cggggatcag taaatccgct
    15541 tgttgcgcgt cggcaagaat atcgctgatc agcggctcgc cttttgtcgt gggcttatct
    15601 gttgccacca gcaactgggg ggccgctttg tcggtagttg tctcgcctgc taatgcttcg
    15661 ctcaacaacg cgagaaaatc ttgtgcagca tcgctggctt tgccgccagg caatgtggtg
    15721 gtgtcaacgt cggcggtaat taagggcgct aagcgaatca ttcaggtttc ctcatggcgg
    15781 cgcgctgggc gaactcatcc atctttttct gatcgaggcg gttttctgca agcagtgccg
    15841 ccgtggatag ccgttcctgc agtgtctgcc aggcctgcaa acgttgtttc ttttctcgcc
    15901 agctgttcag ggcaatgtca actttctgcg tccactgatt aagttgctgg cgatgctgag
    15961 taatggcttt ttccagcgtc tggataaact gctgatagtt gatccagcgg ttgctggtca
    16021 tcccggcact catatcgctg ttgaggttat tgcgatattc attctgataa tcgatcagca
    16081 ttttgagctg ttcttccgcc tgctgacacc cgcgacgcat ttcacccagc aggcgcgcgg
    16141 catcctctac ctctttttct gccagatctt tcagggtcgc cagcgcgcca tgttctgcca
    16201 tctgccgtta tctcctgggt tatgacactg tcgggaaaat acgctccagc ccctggagag
    16261 acgcttccca gtccgcgcgt tcaaaaatgc cttgttgcaa atagccctcc agctgcggcc
    16321 acagggcgat ggctttgtcg agcatcggat cgctgccttt ggcatacgcg ccgacgctaa
    16381 ccagatcgcg gttacgctga aaactcgaca acagctgttt gaaggtgcgc actcgcgcgt
    16441 aatgttgctc actgatcaac gccgtcattg cgcggctgat cgacgcttca atatcgatag
    16501 ccggatagtg cccggcttcc gccagtcggc gagacagcac aatgtgaccg tcgaggattg
    16561 cccgcgcaga gtcggcaatg gggtcctgct ggtcatcgcc ttcagtgagc acggtataaa
    16621 acgcggtaat cgatccgccg ccgctaatgc catttccggc acgctcgacc agtgccggta
    16681 atttggcaaa caccgacggg ggataacctt tagtggcggg tggttcgcca atcgccagcg
    16741 caatttcacg ctgggccatc gcgtagcggg tgagggagtc cataatcagc aacacatgct
    16801 gaccacgatc gcgaaaatct tcggcaatgc gcgtggcata ggcggcaccc tgcattcgca
    16861 ggagaggaga aacatccgcc ggagcggcaa tcaccacgga gcgtgcacgc ccttcggcac
    16921 cgaggatatt ctcaataaaa tcttttactt cgcgtccgcg ttcaccaatc aaacccacga
    16981 caatgacatc ggcgcgggtg taacgtgcca tcatccccag cagcacactt ttaccaacgc
    17041 cggacccggc aaacagcccc atacgctgcc cacgcccaac ggtaagcagg gcattgattg
    17101 ggcgcacgcc ggtgtccagc acatgttcaa tcggtgtacg ttgcaacggg ttaaatggcg
    17161 gggtaatcag cgcaccggtt tccgtcgtat cgggggaggc catcgagcgg tttaccgctg
    17221 ccgtccagaa cacgacctaa taacgccgga ccgagcggta actgtttgcc gctttgcagc
    17281 ccttctgccg aaatgttttt ggcataaaca cgcgcgccgg gcaggacacc ttcgacttcc
    17341 tccagcggca ttaaaaacag ccgttgaccg ttaaagccaa cgacttcact ttctacttcg
    17401 tgcgtttcag tgccgttctg gcgctcaatc acacaggttg cgccgagcgg caattgtaat
    17461 ccggtggctt ccagcaccag cccggtagcg cgggttaatc gcccgtagcg acgtaccgca
    17521 ggcaactgcg ccattttggc ttcaaagtta tccagcgtgg ttagccagcg agtcaggcgc
    17581 gtggtcatta caccactcct ggtgctgcca gacggcagag ttcttgccag cgagtggcga
    17641 cactggcgtc gagatcgcct tcatcggcgg agactttaca gccgccagga tggagggtgg
    17701 gatcgccccg caagcgccag ccatgcaaac ttaaggtagc gccgagcata tcatccacac
    17761 gttgcagatc atccgggtgc acgcgcagct gtggtttacc gctgaataac ggttcttgct
    17821 gcaacaactg ttggatctgt ttgatgagtg ccgagttatc cactgttggc gtctgaccga
    17881 tgacctgacg tgctgcctcc agcgccatct gcatcaggcg cgaagc
//
LOCUS       DADLTD010000073        17663 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLTD010000073 DADLTD010000000
VERSION     DADLTD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.073"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..2017
                     /gene="fhuE"
                     /locus_tag="JN701_001956"
     CDS             <1..2017
                     /gene="fhuE"
                     /locus_tag="JN701_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAY7364413.1"
                     /translation="DYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLEISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="JN701_001957"
     CDS             complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="JN701_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY7364414.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3805..4602)
                     /locus_tag="JN701_001958"
     CDS             complement(3805..4602)
                     /locus_tag="JN701_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7364415.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4613..5617)
                     /gene="holB"
                     /locus_tag="JN701_001959"
     CDS             complement(4613..5617)
                     /gene="holB"
                     /locus_tag="JN701_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY7364416.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5614..6255)
                     /locus_tag="JN701_001960"
     CDS             complement(5614..6255)
                     /locus_tag="JN701_001960"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY7364417.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="JN701_001961"
     CDS             complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="JN701_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY7364418.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="JN701_001962"
     CDS             complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="JN701_001962"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY7364419.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="JN701_001963"
     CDS             complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="JN701_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY7364420.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="JN701_001964"
     CDS             complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="JN701_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7364421.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="JN701_001965"
     CDS             complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="JN701_001965"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY7364422.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDIMVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="JN701_001966"
     CDS             complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="JN701_001966"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY7364423.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEI"
     gene            complement(11667..12620)
                     /locus_tag="JN701_001967"
     CDS             complement(11667..12620)
                     /locus_tag="JN701_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY7364424.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="JN701_001968"
     CDS             complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="JN701_001968"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY7364425.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="JN701_001969"
     CDS             complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="JN701_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY7364426.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="JN701_001970"
     CDS             complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="JN701_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY7364427.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14784..15368
                     /gene="yceF"
                     /locus_tag="JN701_001971"
     CDS             14784..15368
                     /gene="yceF"
                     /locus_tag="JN701_001971"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY7364428.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="JN701_001972"
     CDS             complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="JN701_001972"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY7364429.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            16725..>17663
                     /locus_tag="JN701_001973"
     CDS             16725..>17663
                     /locus_tag="JN701_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HAY7364430.1"
                     /translation="MKYTPVGVGIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMAN"
BASE COUNT         4404 a         4752 c         4312 g         4195 t
ORIGIN      
        1 tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat
       61 tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct
      121 gggcgaagtg atggaaaaca cgctggagat cagcaaaagt caggcggatt ccgatcgtgc
      181 tctttattat tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta
      241 ttttgaatcg cgctggaatc tgggcgatgc actttctgat atggcactgt ttgaacgcgt
      301 agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat
      361 tgatatggtt cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata
      421 cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg
      481 taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta
      541 caacagtgaa aagaccttct tctccggcat tgtcgatgct gatttaggcg atcttacgac
      601 gctttcagcc ggttacgaat atcagcgcat tgatgttaat agccctacct ggggcggttt
      661 accgcgctgg aatactgatg gcagcagcaa cagttacgat cgtgcacgca gtaccgcacc
      721 tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt
      781 tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa
      841 aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag
      901 taattatgga cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa
      961 agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa
     1021 tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt catgggccaa
     1081 catcttcccg gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga
     1141 ctggtcacca cagagcctgg cacaggacga taccacacat atgaaatcgt tatatgctgc
     1201 cactcgagtc acccttgccg atccgctgca tctgatcctc ggcgcacgtt ataccaactg
     1261 gcgggttgat acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct
     1321 ggtgtttgac atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc
     1381 gcaaaatgat cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga
     1441 gctgggtctg aaatcgggct ggatgaatag ccgtctgacc accacgttag ccatcttccg
     1501 tattgagcag gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga
     1561 aaccgcctat aaagcggtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg
     1621 cgcaattacc gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa
     1681 cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta
     1741 tcggttgcct gtcatgccag agttgacagt cggcggtggt gtgaactggc aaaatcgcgt
     1801 gtataccgac accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct
     1861 ggtggatctc ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa
     1921 taacctgttc gacaaaacct acgataccaa catggaaggt tctatcgtct acggcgcacc
     1981 gcgtaatttc agcattaccg gcacgtatca attctgaata acacctgtaa aaaaggcagc
     2041 catctggctg ccttagtctc cccaacgtct tacggattag tggttacgga tgtactcatc
     2101 catctcggtt ttcaggttat cggatttagt accgaaaatc gcctgaacac cagaacccgc
     2161 aaccactacg cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc
     2221 agcaacgctg acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc
     2281 accaaatgca gcaaccagag ccggtgccat ttcgctggta cctgttgctt ttgcatcttc
     2341 agtcgcgtct tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat
     2401 ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct
     2461 gttaccagac agaacgataa agtcgatcag accgtgcgag aacgacgtac cgtcatgcat
     2521 ccccagaaga atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag
     2581 gatcggcgca acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga
     2641 ggtcagcgcc gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga
     2701 gtgccagata gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga
     2761 cagtttaccc gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg
     2821 acctgctgcg ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg
     2881 cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg
     2941 gttctggtaa gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat
     3001 gaaggacagc acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg
     3061 tttaccggca aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat
     3121 gtacgctgcg atcgcaccgg agataatccc tccgagtacg ccagtatccg ccaggtgttt
     3181 agaggcgatt tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt
     3241 aaccatgatg ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa
     3301 gccgagggcg acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc
     3361 ttctgccata acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc
     3421 cagcagaata cctgcgatag gtagtacgga taccggcagc atcagcgatt taccgacctt
     3481 ttgcaggtta gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt
     3541 tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc
     3601 gccctttatt tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac
     3661 gtttcaactt cagttcattg gagaagttgc acattttgac aaaacatatg tcacaatatt
     3721 tcaaacgcgg ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta
     3781 attacgagct ttaaaaaaac tcattcaacg gatggattga aggcgggaag cgtcgatgtg
     3841 aaacagacgg gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt
     3901 caacacagcc atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg
     3961 atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat ccagggggac
     4021 ataacgcgca gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga
     4081 gatgtaaaat ccgagatcca gtaatttacc cgccgtttct ctgtcctctg taaaacagtg
     4141 tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcacg
     4201 ggcgtcacgg gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg
     4261 gatgaaggac tcttgctgac gtactttagt ttccggtgtg taataataat ccagcccggt
     4321 ttcacccagc gctacaacac cctcttctgc cgccagacgg cgtaaatctt ctacatcgta
     4381 gggatcattc tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc
     4441 taccagatcc cgcatatgta aataacccgg taatgttgtg gcgactgcca gacaaaattt
     4501 cacatcgcgt gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata
     4561 atccagacca tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat
     4621 gaggaaccgg tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg
     4681 tgatgagaag ctcgcggttg atgcctgtga cagacattaa ctgttcacga atgtggcaaa
     4741 catcccccag tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcggcgacca
     4801 ggcccggcac atcaacattg gtcacctgtg cagcgccatg atggcgtttt agcgcatcca
     4861 tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcca
     4921 gcagcgaata ccagtcgccc gatggtacgc tatatgccaa cgcctgacac aatgtttcac
     4981 gagcctgcca gttatctccc tgaaacaacg ccagtgccgc gctaggcgaa ccggcactta
     5041 agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca
     5101 cggcgtactg ttccggcggc ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg
     5161 ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt
     5221 caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca tcggttaccc
     5281 agacaacttt cgcaccacct aggcgtgcgt gctcattcag cttttcggtg acctcacgta
     5341 ccgcatcaat gcccagcgta tttttccctt tttcgggagc cagggtgtag taatcgggat
     5401 gcgtgccagc ctgcatcaac tgacatccac gacagtgacc gcaacttttg tggccctgcg
     5461 gttgttggca gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg
     5521 gtaacgcctg aatgagtagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt
     5581 tttcgaaatc aggtcgtaac catggatacc atctcatgcg tccaactcct tcacccagtg
     5641 ggtcacggta gtgcggattg catccatcac ggcctccagc ggctgggtgg catcaatggt
     5701 atgaatactt ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc gattaaagaa
     5761 atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc
     5821 aacttccggg gtaacatcga gatagagcgt taagtcaggg cgaaaatccc cgagaacagc
     5881 atcacgcagt gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg
     5941 agtggagaga tcgtggcgat cgccaatcac ccaggtgccg ttagccagag ctggtttgat
     6001 gaccgtttct accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt
     6061 aatgacttca tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag
     6121 ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt
     6181 ctcaaccacc acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat
     6241 atacttactg cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat
     6301 ggctggcaag attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat
     6361 acggcgtttt tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg
     6421 cacctggcgg caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg
     6481 cacgagaaag tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct
     6541 gcaggcgcat accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac
     6601 tggcaacggc ggtttctttt tcgataattg atgccatcgt caccagctgg tttttatctt
     6661 tataaggcag accgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct
     6721 tgtgcgctcg cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg
     6781 gccagaacca accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg
     6841 cgtacttatc gtcactcagc gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt
     6901 agtcgctcag acgcatccct tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt
     6961 ccagcaattt cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt
     7021 taaagtgaga aagatccggt tcgatacgca gcaaccattg aaaaacccgt gggcgattga
     7081 tgatcttatc ggcataaagc tgttcaccaa gcgccagacg tccggtccct ggcttcaggg
     7141 taaatatcgt ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga
     7201 cgcccacacc agcggcgata cccagtacca ccagcaataa caagataatc aataacactt
     7261 ttttcatgac taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga
     7321 cgaaaaggag acatcgccac aggcacgtac gggcatcact ggcattaacg cattacaaat
     7381 aaccatctca tctgcctgca acgcctcttc cagagaggct tgcacttcga caagctgata
     7441 agaggattgt gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg
     7501 atccagtcgc ggcgtataaa ctacattgcc cttccgccag aacaaattag ccgcacagca
     7561 ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag
     7621 atgagagcgg atcaatactt gctcaagccg attaagatgt ttaatacctg caagatgggg
     7681 attgcgcccc agccgcaccg ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc
     7741 gtaatgtgca ggataaaccg taacggagag aatccgcgtt gccggtccac tgttcaatgt
     7801 gctgtaccct cgcccgccac taccgcgact gatcacgact ttcagcacac cattttgctg
     7861 ttctgctgcc agcgttttca tctcttgttc aagctgaggc cagaagtcac agaaaatcat
     7921 caaccgctga caagcatcct gtagtcgctg gatatgcgcc gataacaaac tgactttacc
     7981 gtcgataact ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac
     8041 tgccagcgat tcctgcttat gaccgttaat taagaacata ccggctcctt atcagagaat
     8101 tgttagtgtg gcagcatgct cactacggaa caagtcggaa taaaagctaa gaaaaaaggc
     8161 ccgcaagcgg accttttata tgggtgggaa atgacaactt agatcttttt aaagatcaaa
     8221 gaaccattag tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc
     8281 tgacgtgctt catgcggtac gaaatccaga tcgcaacctt catccaggtt atccaggttg
     8341 atggttggcg gaacagcctg atcgcgcagc gccaggatgg agtagataga ctctactgcc
     8401 cccgccgcac ctaacaggtg accggtcata gatttcgtgg agcttaccaa cacacggctt
     8461 gcagcttcac cgaagatggc tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta
     8521 gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga
     8581 gcatttgcca ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa
     8641 gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc
     8701 gcgtgttcgt actcttctag taccagcata ccggcaccat cgcccagtac gaagccatca
     8761 cgctctttat cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca
     8821 cgtgccgcgc caaaaccacc aacgcccagc ggcgtactgg ctttctctgc gccacctgca
     8881 accatcacgt cagcatcgcc atacgcgata atacgcgcag catgaccaat gttgtgcacg
     8941 ccggaagtac aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc
     9001 agatgacctg ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt
     9061 ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg
     9121 gagccaattg cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc
     9181 tgcatggcct gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt
     9241 tctttgcgcg agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt
     9301 gcataggcgc tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga
     9361 gctttccagg tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca
     9421 actacacgac gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac
     9481 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
     9541 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
     9601 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
     9661 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
     9721 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
     9781 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
     9841 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
     9901 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
     9961 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    10021 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    10081 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    10141 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    10201 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    10261 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    10321 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    10381 ttggtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    10441 gtgataccgg cattattgac catgatatcc acttcaccaa attctgcgcg aattttttcc
    10501 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttagca
    10561 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    10621 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    10681 accagtgcga tttttccttc aaaattcatg attttcctct tttaaatctc gagcgccgct
    10741 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    10801 aggccagtta gcactttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    10861 atgtactcaa cagacttcgt ccactggacc gggttataca actgacgtac cagtgcgtca
    10921 cgaatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac aggaactgtt
    10981 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    11041 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gcccgcagct
    11101 ttacaggcag cgccagcacg ctcaaccgcc tctttatgac cggcaataac cacctgtccc
    11161 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    11221 gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc ttctggtacg
    11281 gcttcttgca tgaacttgcc gcgcatctca accagacgca ccgcatcagc gaaatcaatc
    11341 acgccagcgc aaaccaacgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    11401 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    11461 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    11521 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    11581 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    11641 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat
    11701 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    11761 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccaacgtc
    11821 accacgacat tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    11881 ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt cgccgccagc
    11941 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    12001 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    12061 tcaccataac taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca
    12121 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    12181 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    12241 ttcacgtatt gattggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg
    12301 tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg
    12361 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    12421 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    12481 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    12541 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    12601 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    12661 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    12721 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    12781 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    12841 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    12901 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    12961 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    13021 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    13081 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    13141 gattgtttta agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc
    13201 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    13261 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    13321 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    13381 ctcaatcccc tccaggggcc tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    13441 accggcactg acacaggctt gcgctcgacc ttctttaacc agctccaggg ccatacgcat
    13501 tgaactccca cgactagcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    13561 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    13621 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    13681 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    13741 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    13801 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    13861 ccgcggtagt aaccgtcggc agtgatatgg tgacgcaggt gtttttcacc agaagttttg
    13921 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    13981 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    14041 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    14101 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    14161 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    14221 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    14281 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    14341 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    14401 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    14461 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    14521 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt
    14581 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    14641 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    14701 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    14761 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    14821 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg
    14881 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    14941 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    15001 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    15061 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    15121 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgtgcgagg
    15181 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    15241 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    15301 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    15361 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc
    15421 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    15481 tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    15541 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    15601 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    15661 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    15721 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    15781 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    15841 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgactgcgc
    15901 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    15961 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    16021 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    16081 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    16141 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    16201 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    16261 agtttatatt caggcttaat acgttttttg ttcacccgca cttcgccttt acgcaaaata
    16321 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt gtcgatacgt
    16381 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    16441 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    16501 gttttacacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    16561 cagcaagaag tgaaaaatgt aatgaacgca tcccacacaa ttaaacacgg agcagtatga
    16621 tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag atttccggca tcatagtgcc
    16681 caatacggga tgcataaagc tccctcagac tggagactcc acaaatgaaa tatacaccgg
    16741 ttggcgttgg tatcgcaaaa catgtcattc agattcactt catcaatgag cacacaggtg
    16801 aagtggttga taaacagttg cgtagacagg attttctgac gttcttcggc aaccgtgagc
    16861 catgcctgat tggtatggag gcctgtggag gttctcagca ctgggcacgg gaactgacaa
    16921 aacttggtca taaagtccgg ttgttgcagg cccgcttcgt taaggcattc gtcatgggca
    16981 ataagaatga tgtgatggat gcccgggcta tctggatggc ggttcagcag ccgggtaaag
    17041 aaatcgccgt aaaaacagaa gaacagcagt cggtactggt tctgcaccgt acccgcatgc
    17101 aactggtgaa gttccggacc gcacaaatta atgccctgca cgggacgtta ctggagtttg
    17161 gtgaaaccat ccacaaaggc cgggcagcga tggagcggga gttccccgaa gcactggaac
    17221 ggatgaaaga gagactgcca ccgtatctca ttatggttct ggaaaaccag tacaaccgac
    17281 tgaatgagct ggactcactg atagaggata ttgaaaaaca gcttaccagc gtggcgaggc
    17341 agaatgaaac ctgtaagcgg ttgctggata ttcctggcgt tggaccactt attgcgacgg
    17401 cagcggtggc caccatgggg gaagcatcag cgtttaaatc ggggcgagag ttcgccgcat
    17461 atgttggtct ggttccaaaa caaactggct ccggagggaa agtacgtctg ctggggataa
    17521 gcaaacgtgg tgacacttat ctcaggacat tatttatcca cggtgcaaga gcggtggcat
    17581 tagtagctaa agagcctggc ccgtggataa ccgaactgaa aaaacgtcgt ccagccagtg
    17641 tggcaatcgt cgccatggca aac
//
LOCUS       DADLTD010000074        17578 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLTD010000074 DADLTD010000000
VERSION     DADLTD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.074"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            5..1138
                     /gene="blaEC"
                     /locus_tag="JN701_001974"
     CDS             5..1138
                     /gene="blaEC"
                     /locus_tag="JN701_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7364431.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1227..1760
                     /locus_tag="JN701_001975"
     CDS             1227..1760
                     /locus_tag="JN701_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7364432.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..2074)
                     /locus_tag="JN701_001976"
     CDS             complement(1757..2074)
                     /locus_tag="JN701_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7364433.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2256..2402)
                     /gene="ecnB"
                     /locus_tag="JN701_001977"
     CDS             complement(2256..2402)
                     /gene="ecnB"
                     /locus_tag="JN701_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7364434.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2513..2638)
                     /gene="ecnA"
                     /locus_tag="JN701_001978"
     CDS             complement(2513..2638)
                     /gene="ecnA"
                     /locus_tag="JN701_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7364435.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2690..3256)
                     /gene="efp"
                     /locus_tag="JN701_001979"
     CDS             complement(2690..3256)
                     /gene="efp"
                     /locus_tag="JN701_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7364436.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3298..4347
                     /gene="epmB"
                     /locus_tag="JN701_001980"
     CDS             3298..4347
                     /gene="epmB"
                     /locus_tag="JN701_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7364437.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            4721..5590
                     /locus_tag="JN701_001981"
     CDS             4721..5590
                     /locus_tag="JN701_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364438.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(5793..6146)
                     /locus_tag="JN701_001982"
     CDS             complement(5793..6146)
                     /locus_tag="JN701_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY7364439.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(6284..7930)
                     /gene="groL"
                     /locus_tag="JN701_001983"
     CDS             complement(6284..7930)
                     /gene="groL"
                     /locus_tag="JN701_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY7364440.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(7974..8267)
                     /locus_tag="JN701_001984"
     CDS             complement(7974..8267)
                     /locus_tag="JN701_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY7364441.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8543..9799
                     /gene="yjeH"
                     /locus_tag="JN701_001985"
     CDS             8543..9799
                     /gene="yjeH"
                     /locus_tag="JN701_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY7364442.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(9815..10291)
                     /gene="fxsA"
                     /locus_tag="JN701_001986"
     CDS             complement(9815..10291)
                     /gene="fxsA"
                     /locus_tag="JN701_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY7364443.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            10628..12064
                     /gene="aspA"
                     /locus_tag="JN701_001987"
     CDS             10628..12064
                     /gene="aspA"
                     /locus_tag="JN701_001987"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY7364444.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            12182..13483
                     /locus_tag="JN701_001988"
     CDS             12182..13483
                     /locus_tag="JN701_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7364445.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            13599..13937
                     /gene="cutA"
                     /locus_tag="JN701_001989"
     CDS             13599..13937
                     /gene="cutA"
                     /locus_tag="JN701_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY7364446.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            13913..15610
                     /locus_tag="JN701_001990"
     CDS             13913..15610
                     /locus_tag="JN701_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY7364447.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            15647..16222
                     /locus_tag="JN701_001991"
     CDS             15647..16222
                     /locus_tag="JN701_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7364448.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            16329..16404
                     /locus_tag="JN701_001992"
     tRNA            16329..16404
                     /locus_tag="JN701_001992"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16362..16364,aa:Phe,seq:gaa)
     gene            16602..>17578
                     /locus_tag="JN701_001993"
     CDS             16602..>17578
                     /locus_tag="JN701_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7364449.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         4344 a         4529 c         4297 g         4408 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgat gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc agatgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtaccgcc cgcagaagaa aagaattacg cctggggata
      661 tcgcgaaggc aaggcagttc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgcagttctc ctacgccgcc gcgtggcgtg acagtagtga
     1321 ataatttcga cgctaaacgc tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag tctgcgtgat ggcggcggcc
     1441 tgaatgtcat caataaaggc tataaccctg acagagaaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaacacgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtc attgcacttg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccaggcgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tagccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct actggtaacg
     1921 atttcatcgc ccaggcaagt agcgccatac tgacaatcat cgccgtcacg gtaataacac
     1981 tcggcgtcaa acgactaaag ccgtgggtat atttcaggcc aacggcccat accacttcca
     2041 gcagaccagc aataactaag ataatccagg acatatcagg ctccggagaa ttggggccgt
     2101 ccccggtgaa aagacgcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt
     2161 tgccaggtgg caggggaatt tcaactatta ccttagtgac tatcgcagaa aaaacaaacg
     2221 gcacgacaca gctgtcgtac cgttattgct tattgctatt gctgcgcttt cgttgctgcg
     2281 ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag
     2341 gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt cttcttcacc
     2401 ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt aataatagac
     2461 aacatcgcca aaaatgacgg aaatctgatg atttttcgga agagaagaaa aattagctgg
     2521 cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg cgagcggtgt
     2581 tacagcccgt gagcagcgta ctggcaagca aaacaagaac gataagtcgt ttcatcatct
     2641 ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat tacttcacgc
     2701 gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca
     2761 gcggaacctt aaccacagcg ccagtagaca gggtagccgg tttgccgcca gtacctgcgg
     2821 tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag ttcggcggag
     2881 taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga tccagcagcc
     2941 atttagcgtt atcgccaatt gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt
     3001 tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata tcgacaacat
     3061 cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta ccggtcagca
     3121 gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg aattcactcg
     3181 cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga aaatcgttgc
     3241 tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg
     3301 gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca acttgccgat
     3361 gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga cgaaaaactg
     3421 ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc atttatcgat
     3481 cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac ctcgcaagat
     3541 gagtttgtcg tcgcgtccgg attctccacc gacccactgg aagaacagca cagcgtagtg
     3601 cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg
     3661 gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag
     3721 cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga cgagatgatt
     3781 ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt gctcacacaa
     3841 ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc gattgtgatc
     3901 ccggcacgca tcacagatgc gctggttgaa cgcttttcac attctacgct gcaaatcctg
     3961 ctggtgaatc acatcaacca tgccaatgag atagatgaaa cattccgtca ggcgatggct
     4021 aagttgcgcc gggtaggcgt tactttgctg aaccagagcg ttctgttacg tgatgtgaac
     4081 gataacgcac aaacgctggc aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg
     4141 tattacctgc atgtgctcga taaagtacag ggcgcagcgc attttatggt gagtgatgac
     4201 gaagcacggc agattatgcg tgagttgctg acattggtgt cgggatatct ggtgccgaaa
     4261 ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc tggatctcca gctacgccag
     4321 cagttactga ttaaacaggg aagttaaaca ttaaaattgc ctgatgcgct acgcttatca
     4381 ggcctacatc gtgcattgaa attgaatgga tttgcaatga actgtaggcc ggataaggcg
     4441 tttacgccgc atccggcaag agcaacaaac gcaagtacag caacctgtca ttaaactgag
     4501 aaatttaatc tttgcccgtt gcataacctt taattattca tatgcattgc tgttgttact
     4561 ttttatatag ctaacaccaa taattaacct ttcttttctg tttatataga gtgagcacgg
     4621 ctatttatgt tattttaaca actattacta ccaagcccat ttatctggct tataaattaa
     4681 aattaattat ttaattgaga aatggttagg gagaacctac atggccataa gcatcaaggg
     4741 tgttaatacc ggtgttattc gcaagagtaa taatttcatt gcactggcgc tgaaaattaa
     4801 agaacctcgc aataaagaat cgttgttctt tatgtctgtc atggaactgc gcgacctgct
     4861 gattgcgctg gaaagtcgtc tgcatcaaaa gcataaactg gatgctgctg cacgccttca
     4921 gtatgagcaa gctcgcgata aagtcattaa gaaaatggca gaaaacatcc cagaaattct
     4981 ggttgatgag cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga
     5041 taatcaaggc gaaaatctta ccttcgtatt aacgctacat gacggtagca aatgtgagtt
     5101 agtcgtcaat gagttgcaga ttgaaatgct agcgcgagca atcattcatg caattaataa
     5161 tgcggaaatg cgtgaactgg cattacgtat tacctcactg ttagatttcc tgccgttata
     5221 tgacgttgac tgccaggaaa atggcaatct ggaatatgac acctattccc aaccggaatg
     5281 gaaacataac ttgttcgacc attatctggc ggtgctttac cgctttaaag atgaaagtgg
     5341 caaagaacaa tttagcggcg cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaagt
     5401 agaggcgatc actcgccgaa tgcttgattt cagcccacga ctgaaaaaac tcgcgggtgt
     5461 gccatgtcag gtctatgttc gcaccgtagc ggcgaataac gcccaaccgc taacccagga
     5521 tcaatgctta cgcgcgctgc atcacttacg tgttcaatcc accagcaaaa ctgcaccaca
     5581 ggcgaaataa tctcatcttt gctaatgcga ggctggagcc atccagcctg aaatgactga
     5641 taacgtgatc gttggtcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct
     5701 caagcattca atcagttgca atgcctgctg taggcctgat aagcgaagcg catcaggcaa
     5761 taatacgttt gtcacccagc ctctggatca catcagttcg ggcacttata aacctgaccg
     5821 ataatctggc tatccgtcgg gacaaaactg gacaacattc cctgcgacgg gctgctgatg
     5881 ccataaatca cgttaccgcc cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg
     5941 cgcatagaac cgccctcttc tccgtgttgc ccggaaagcc agttgctttg cttacctgtc
     6001 gcagtaccaa tcagctggca ctctgcgcct ggctgctcgt ccacaatgcg tacactctga
     6061 ccggcagcac tcaattcgtt gctggagcta caacccgcca tcagtagcgc ggcaccgaca
     6121 atccctgcta agtattttac gtgcatgtta ttccccataa tcaatgagct ggacgccact
     6181 cgcgtcgtcc gtgtctgaat cttatactag aaagatgacc aaaagaaaaa cccccagaca
     6241 tttctgcccg ggggtttgtt tatttctgcg aggtgcaggg caattacatc atgccgccca
     6301 tgccacccat gccgcccata ccgccagcag cgcctaagtc agctgcatcg tttttcggca
     6361 ggtcggtaac catgcactcg gtggtgatca tcaggccagc cacagaagcc gcgtactgca
     6421 gagcagaacg ggttactttg gttggatcca agatacccat gtcgatcatg ttgccgtatt
     6481 cttcggttgc tgcgttgtaa ccgtagttgc cgtcgccgcc tttaacagtg ttagcaacaa
     6541 cagacggttc ttcgccgcag ttcaatacga tctgacgcag cggagcttcc attgcacgca
     6601 gtgcaacttt gatacccacg ttctggtctt cgttctgacc acgcaggtca gccagtttag
     6661 acgctacgcg gatcagcgca acaccaccac cagcaaccac gccttcttct accgcagcac
     6721 gggtcgcgtg cagggcatct tcaacgcgtg cttttttctc tttcatttca acttcggtag
     6781 cagcacctac tttgataact gcaacgccgc ctgccagttt cgctacgcgc tcctgcagtt
     6841 tttcacggtc gtagtcagaa gttgcttctt caatctgctg acggatctga gcaacacggc
     6901 cctggattgc agcttcttca cccacgccat caatgatggt ggtggtgtct ttgttgatca
     6961 caacacgttt agcctgaccc aggtcttcca gggttgcttt ttccagctcc ataccgatct
     7021 cttcagagat tacggtaccg ccagtcaggg ttgcgatatc ctgcagcata gctttacggc
     7081 gatcgccgaa gcccggagct ttaaccgcag caactttcac gatgccacgc atggtgttaa
     7141 caaccagagt tgccagcgct tcgccttcaa catcttcagc gatgatcagc agcggtttac
     7201 ctgctttagc aacggcttcc agaaccggca gcatttcgcg gatgttggag attttcttgt
     7261 cagccagcag gatgaacggg ctttccagtt ctactgcgcc agtttccggc ttgttgatga
     7321 agtaaggaga caggtagcca cggtcgaact gcataccttc aaccacgtcc agttcgtcct
     7381 gcagaccggt accgtcttca acggtgataa cgccttcttt accgactttg tccatcgctt
     7441 cagcgatcag tttacctacg gtttcgtcgg agttagcgga gatggtacca acctgagcaa
     7501 tcgctttaga gtcagagcat ggtacggaca gcgctttcag ttcttcaact gcagcggtaa
     7561 cagctttgtc gataccacgt ttcaggtcca tcgggttcat gcccgcagca actgctttca
     7621 gaccctcagt gatgatagcc tgagccagta cggttgcagt ggtggtaccg tcgcctgcag
     7681 cgtcgttcgc tttagaggca acttctttca ccatctgcgc acccatattt tcgaacttgt
     7741 cttccagttc gatttcacga gcaacggaaa caccatcttt ggtgatggtc ggtgcaccga
     7801 aagatttatc cagaactacg ttacggcctt tcggaccgag ggtaactttc actgcatctg
     7861 ccagtacgtt tacgccgcgc agcattttca cacgagcgtc gttaccgaat tttacgtctt
     7921 tagctgccat tatctttatt ccttaaattc gtatgttcag tgtcgtgcga ggattacgct
     7981 tcaacaattg ccagaatgtc gctttcggac atgatcaaca cttcttcatt gtcgatcttc
     8041 tcagatttca caccgtagcc atcgttgaaa ataacgatgt cgccaacttt cacatccagc
     8101 ggcttcactt cgccattttc aaggatacgg ccattgccga cagccagcac ttcgccgcgg
     8161 gtggatttag ccgctgcaga gccggtcaga acgatgccgc cagcagattt agtttcaact
     8221 tctttacgct tgacgatcac gcgatcatgc aatggacgaa tattcattga taactctcct
     8281 ttgagaaagt ccgtatctgt tatgggtgac gccggagctt acgtggtttc ccggctggtg
     8341 accagagaaa tggggatgag gattcgcccc ttcaaggggg aaaaagaaaa aaaattctga
     8401 tcagcacaaa atcgggtgaa aaccctgatt cacctcacat ttcatcgcaa tttcttcatc
     8461 gccgtcataa gcgaatctga ttgtgctacc atcgaaaatc tacgcagttg cccaaaattt
     8521 gggcgcaatc ggatatcaac ccatgagtgg actcaaacaa gaactggggc tggcccaggg
     8581 cattggcctg ctatcgacgt cattattagg caccggcgtg tttgccgttc ctgcgttagc
     8641 tgcacaggta gcaggcaata acagcctgtg ggcgtggccc gttttgatta tcttagtgtt
     8701 cccgattgcg attgtgtttg cgattctggg tcgccactat ccctgcgcag gcggtgtcgc
     8761 gcacttcgtc ggtatggcgt ttggttcgcg gcttgagcga gtcaccggct ggttgttttt
     8821 atcggtcatt cccgtgggtt tgccagccgc gctacaaatt gccgccgggt tcggccaggc
     8881 aatgtttggc tggcatagct ggcaactgtt gttggcagaa ctcggtacgc tggcgctggt
     8941 gtggtatatc ggtactcgca gtgccagttc cagtgctaat ctacaaaccg ttattgccgg
     9001 acttatcgtc gcgctgattg tcgctatctg gtgggcgggc gatatcaaac ctgcgaatat
     9061 ccccttcccg gcacctggta atatcgaact taccgggtta tttgctgcgt tatcagtgat
     9121 gttctggtgt tttgtcggtc tggaagcatt tgcccatctt gcctcggaat ttaaaaatcc
     9181 agagcgtgat tttcctcgcg cattgatgat tggtctgctg ctggcaggat tagtctactg
     9241 gggctgtacg gtagtcgtct tacacttcga cgcctatggt gaaaaaatgg cggcggcagc
     9301 atcgcttcca aaaattgtag tgcaactgtt cggtgtagga gcgttatgga ttgcctgcgt
     9361 gattggctat ctggcctgct ttgccagtct caacatttat atacagagct tcgcccgcct
     9421 ggtctggtcg caggcgcaac ataatcctga ccactacctg gcacgcctct cttctcgcca
     9481 tatcccgaat aatgccctca atgcggtgct cggctgctgt gtggtgagca ctttggtgat
     9541 tcatgcttta gagatcaatc tggacgctct tattatttat gccaatggca tctttattat
     9601 gatttatctg ttatgcatgc tggcaggctg taaattattg caaggacgtt atcgactact
     9661 ggcggtggtt ggcgggctgt tatgcgttct gttactggca atggtcggct ggaaaagtct
     9721 ctatgcgctg atcgtgctgg cggggttatg gctgttgctg ccaaaacgaa aaacgccgga
     9781 aaatggcata atcacataat ccggcgtttc gacattaatc ctggcgatcg tctttatgat
     9841 caaggcggtc gcgctcatca tcctttcgct ggtactcacc atcaaaagta ttaccgccac
     9901 cggtcccggc gctaaaaccg ccgcctggca tacgagaaaa gcgcaaatgc ggcatcaact
     9961 tcactgtcag atgcttttgc accggcggta ataaaagcag aagaccgagg aagtcggtga
    10021 aaaagcccgg taataaaagc agcaaaccgg caatgatcag cgaaacactt ttaatcatct
    10081 ccgccgctgg gttttcaccc gccgccattt tttgctgcat cagcacgaaa ttcttaaagc
    10141 cctggttacg taccagtgac atcccgataa ctgacgtgaa tataaccagc acgagggtca
    10201 gcaatacccc caatacatgg gcaacctgaa taaagattga aatctcaata tagacataaa
    10261 ggaaaatggc aataaaaggt aaccagcgca aaggtttctc ctgtaatagc agccggtaaa
    10321 acccggctac ctgaatgggt tgcgaatcgc gttcagctta tattgtggtc attagcaaaa
    10381 tttcaagatg tttgcgcaac tatttttggt agtaatccca aagcggtgat ctatttcaca
    10441 aattaataat taaggggtaa aaaccgacac ttaaagtgat ccagattacg gtagaaatcc
    10501 tcaagcagca tatgatctcg ggtattcggt cgatgcaggg gataatcgtc ggtcgaaaaa
    10561 cattcgaaac cacatatatt ctgtgtgttt aaagcaaatc attggcagct tgaaaaagaa
    10621 ggttcacatg tcaaacaaca ttcgtatcga agaagatctg ttgggtacca gggaagttcc
    10681 agctgatgcc tactatggtg ttcacactct gagagcgatt gaaaacttct atatcagcaa
    10741 caacaaaatc agtgatattc ctgaatttgt tcgcggtatg gtaatggtta aaaaagccgc
    10801 agctatggca aacaaagagc tgcaaaccat tcctaaaagt gtagcgaatg ccatcattgc
    10861 cgcatgtgat gaagtcctga acaacggaaa atgcatggat cagttcccgg tagacgtcta
    10921 ccagggcggc gcaggtactt ccgtaaacat gaacaccaac gaagtgctgg ccaatatcgg
    10981 tctggaactg atgggtcacc agaaaggtga atatcagtac ctgaacccga acgaccatgt
    11041 taacaaatgt cagtccacta acgacgccta cccgaccggt ttccgtatcg cagtttactc
    11101 ttccctgatt aagctggtag atgcgattaa ccaactgcgt gaaggctttg aacgtaaagc
    11161 tgtcgaattc caggacatcc tgaaaatggg tcgtacccag ctgcaggacg cagtaccgat
    11221 gaccctcggt caggaattcc gcgctttcag catcctgctg aaagaagaag tgaaaaatat
    11281 ccaacgtacc gctgaactgc tgctggaagt taaccttggc gcaacagcaa tcggtactgg
    11341 tctgaacacg ccgaaagagt actctccgct ggcagtgaaa aaactggctg aagtcactgg
    11401 cttcccatgc gtaccggctg aagacctgat cgaagcgacc tctgactgcg gcgcttatgt
    11461 tatggttcac ggcgcgctga aacgcctggc tgtgaagatg tccaaaatct gtaacgacct
    11521 gcgcttgctc tcttctggcc cacgtgccgg cctgaacgag atcaacctgc cggaactgca
    11581 ggcgggctct tccatcatgc cagctaaagt aaacccggtt gttccggaag tggttaacca
    11641 ggtatgcttc aaagtcatcg gtaacgacac cactgttacc atggcagcag aagcaggtca
    11701 gctgcagttg aacgttatgg agccggtcat tggccaggcc atgttcgaat ccgttcacat
    11761 tctgaccaac gcttgctaca acctgctgga aaaatgcatt aacggcatca ctgctaacaa
    11821 agaagtgtgc gaaggttacg tttacaactc tatcggtatc gttacttacc tgaacccgtt
    11881 catcggtcac cacaacggtg acatcgtggg taaaatctgt gccgaaaccg gtaagagtgt
    11941 acgtgaagtc gttctggaac gcggtctgtt gactgaagcg gaacttgacg atattttctc
    12001 cgtacagaat ctgatgcacc cggcttacaa agcaaaacgc tatactgatg aaagcgaaca
    12061 gtaatcgtac agggtagtac aaataaagaa ggcacgtcag atgacgtgcc ttttttcttg
    12121 tgaacagtaa cttaaaaaca acaatctaat atcaacttgt taaaaaacaa ggaaggctaa
    12181 tatgctagtt gtagaactca tcatagtttt gctggcgatc ttcttgggcg ccagattggg
    12241 gggaataggt attggttttg caggcggatt gggggtgctg gttcttgccg ctattggcgt
    12301 taaacccggt aacatcccgt tcgatgtcat ttccattatc atggcggtta tcgccgctat
    12361 ttctgccatg caggttgctg gcggtctgga ctatctggtt catcagacag aaaagctgct
    12421 gcgccgtaac ccgaaataca tcacgatcct cgcacctatc gtgacctatt tcctgacaat
    12481 ctttgctggt actggcaaca tctctctggc gacactgcca gttatcgctg aagttgcgaa
    12541 ggaacaaggc gttaaacctt gccgtccgct ctctactgca gtggtatccg cgcagattgc
    12601 gatcaccgca tcgccaatct cagcggcagt ggtttacatg tcttccgtga tggaaggtca
    12661 tggcatcagc tacctccatc tgctctccgt ggtcatcccg tccaccctgc tggcggttct
    12721 ggtgatgtcc tttctggtca ctatgctgtt caactccaaa ctctctgacg atccgattta
    12781 tcgcaagcgt ctggaagagg gcctggttga actgcgcggt gaaaagcaga ttgaaatcaa
    12841 atccggtgca aaaacgtccg tctggctgtt cctgctgggc gtagttggcg tggttatcta
    12901 tgcaatcatc aacagcccaa gcatgggtct ggttgaaaaa ccgctgatga acaccaccaa
    12961 cgcaatcctg atcatcatgc tcagcgttgc aactctgacc accgttatct gtaaagtcga
    13021 taccgacaac atcctcaact ccagcacctt caaagcaggt atgagcgcct gtatttgtat
    13081 cctgggtgtt gcgtggctgg gcgatacttt cgtttccaac aacatcgact ggatcaaaga
    13141 taccgctggt gaagtgattc agggtcatcc gtggctgctg gccgtcatct tcttctttgc
    13201 ttctgctctg ctgtactctc aggctgcaac cgcaaaagca ctgatgccga tggctctggc
    13261 actgaacgtt tcaccgctga ccgctgttgc ttctttcgct gcggtgtctg gtctgttcat
    13321 tctgccgacc tacccgacgc tggttgctgc ggtacagatg gatgacacgg gtactacccg
    13381 tatcggtaaa ttcgtcttca accatccgtt cttcatcccg ggtactctgg gtgttgccct
    13441 ggccgtttgc ttcggcttcg tgctgggtag cttcatgctg taatgatcaa tcgcggggcg
    13501 ttcacgcccc gctttctttc ccgccgacta acatccttcc cccgtacgtt gtatagtgac
    13561 ctctctcttg cggttccatc tgttcttgcg aggtgtttat gcttgatgaa aaaagttcga
    13621 ataccgcgtc tgtcgtggtg ctatgtacgg caccagatga agcgacagcc caggatttag
    13681 ctgccaaagt gctggcggaa aaactggcgg cctgcgcgac cttgatcccc ggcgctacct
    13741 ctctctatta ctgggaaggt aagctggagc aagaatacga agtgcagatg attttaaaaa
    13801 ctaccgtatc tcaccagcag gcactgctgg aatgcctgaa gtctcatcat ccatatcaaa
    13861 ccccggaact tctggtttta cctgttacac acggagacac agattacctc tcatggctca
    13921 acgcatcttt acgctgatcc tgctactttg cagcacttcc gtttttgccg gattattcga
    13981 cgcgccggga cgttcacaat ttgtccccgc ggatcaagcc tttacttttg attttcagca
    14041 aaaccaacat gaccttaatc tgacctggca gatcaaagac ggttactacc tctaccgtaa
    14101 acagatccgc attacgccgg aacacgcgaa aattgccgac gtgcagctgc cgcaaggcgt
    14161 ctggcatgaa gatgagtttt acggcaaaag cgagatttac cgcgatcggc tgacgcttcc
    14221 cgtaaccatc aaccaggcga gtgcgggagc gacgttaact gtcacctacc agggctgtgc
    14281 tgatgccggt ttctgttatc cgccagaaac caaaaccgtt ccgttaagcg aagtggtcgc
    14341 caacaacgca gcgtcacagc ctgtgtctgt ttcgcagcaa gagcagcaca ccgcgcaatt
    14401 gcccttttcc gcgctctggg cgttgttgat cggtattggt atcgccttta cgccatgcgt
    14461 gctgccaatg tacccactga tttctggcat cgtgctgggt ggtaaacagc ggctctccac
    14521 tgccagagca ttgttgctga cctttattta tgtgcagggg atggcactga cttacacggc
    14581 gctgggtctg gtagttgccg ccgcagggtt acagttccag gcggcgctac agcacccata
    14641 cgtgctcatt ggcctcgcca tcgtctttac cttgctggcg atgtcaatgt ttggcttgtt
    14701 tactctacaa ctcccctctt cgctgcaaac acgtctcacg ttgatgagca atcgccaaca
    14761 gggcggctca cctggcggtg tgtttgttat gggggcgatt gccggactta tctgttcacc
    14821 atgcaccacc gcaccgctta gcgcgattct gctgtatatc gcccaaagcg ggaacatgtg
    14881 gctgggcggc ggcacgcttt atctctatgc gttgggcatg ggcctgccgc tgatgctaat
    14941 taccgtcttt ggtaaccgct tgctgccgaa aagcggcccg tggatggaac aagtcaaaac
    15001 cgcgtttggt tttgtgatcc tcgcactgcc ggtcttcctg ctggagcgag tgattggtga
    15061 tgtatgggga ttacgcttgt ggtcggcgct gggtgtcgca ttctttggct gggcctttat
    15121 caccagccta caggctaaac gcggctggat gcgcgtggtg caaataatcc tgctggcagc
    15181 ggcattggtt agcgtgcgcc cacttcagga ttgggcattt ggtgcgacgc ataccgcgca
    15241 aactcagacg catctcaact ttacacaaat caaaacggta gatgagttaa atcatgcgct
    15301 cgttgaagcc aaaggcaaac cggtgatgtt agatctttat gccgactggt gcgtcgcctg
    15361 taaagagttt gagaaataca ccttcagcga cccgcaggtg caaaaagcgt tagcagacac
    15421 ggtcttactt caggccaaca tcacggccaa cgacgcacaa gatgtggcgc tgttaaagca
    15481 tcttaatgtc cttggcctac cgacaattct cttttttgac ggacaaggcc aggagcatcc
    15541 acaagcacgc gtcacgggct ttatggatgc tgaaaccttc agcgcacatt tgcgcgatcg
    15601 ccaaccgtga acgacacttg cagcgggaaa gacggaggaa aatagcgtgc aacgtgaaga
    15661 tgtactggga gaagccctga aattattaga attacaaggg attgccaaca ccacgctgga
    15721 gatggttgct gaacgtgtgg attatccact ggacgagcta cgccgcttct ggccagacaa
    15781 agaggcaatc ctctacgatg cgctgcgtta tcttagccaa cagatagatg tctggcgtcg
    15841 tcagctgatg ctggacgaaa cgcaaaccgc cgaacaaaag cagctggcac gttatcaggc
    15901 gttatcggag tgcgttaaaa acaaccgcta tccgggctgt ctatttatcg ctgcctgtac
    15961 gttttatccc gatcctggcc accctattca tcaactggcc gatcagcaaa aaagcgcggc
    16021 ctacgatttc acccacgaac tgttaaccac actggaagtt gacgatccgg cgatggtagc
    16081 aaagcagatg gaactggtgc tggaaggctg tttaagccga atgctggtga atcgtagcca
    16141 ggcggatgtc gacactgcac atcggctggc ggaagatatc ctgcgcttcg cccgctgccg
    16201 ccagggtggt gcattgacct gacagaaaca cagaaaagaa gcgatttgcc gcaatcttaa
    16261 gcagttgaat cgcttttact gaaattaggt tgacgagatg tgcagattac ggtttaatgc
    16321 gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc cgtgtccttg
    16381 gttcgattcc gagtccgggc accaaattca tatcaacgga cctctacgga ggttcgtttt
    16441 tcgtttcaga acgccacgat ttaagcgttc tgcctacaaa tcaattctac cgaactcaac
    16501 cagattcccc ccacatcaac cccattgtgt gggtataatt gcgggcatac cccagttcga
    16561 cagaatttgt accctcttta acgcattcag aaggctgaac tatggcactg actgacgcaa
    16621 aaatccgggc tgcaaagccc actgacaagg cttataaact cactgacggg actggcatgt
    16681 tcctgctggt acatcctaat ggctcccgtt actggcgtct ccgttatcgt attctgggta
    16741 aggagaagac tctggcactt ggtgtgtatc cagaagtttc tctctccgaa gctcgtacaa
    16801 aacgggatga ggcccgaaaa ctgatttcgg agggggttga cccttgcgaa cagaaaagag
    16861 ctaaaaaagt agtccctgat ttacagctct cttttgaaca tattgcgcga cgctggcatg
    16921 ccagtaataa acaatgggca caatcacaca gcgataaagt actcaaaagc ctcgagactc
    16981 acgttttccc ctttatcggc aaccgggata tcacaacact caataccccg gatctgctta
    17041 tccctgttcg tgctgcagaa gctaaacaaa tttatgaaat cgccagtcgt ctgcagcaaa
    17101 gaatatctgc cgtaatgcgt tatgccgtac agtctggcat catcagatat aatcctgctc
    17161 tggatatggc tggcgcattg actacggtaa aacgccagca tcgccccgct cttgatcttt
    17221 ctcgcctgcc tgaacttttg tcgcgtattg gcagttacaa agggcaaact gtcacccagc
    17281 ttgccgttat gctgaattta ctggttttta ttcgttccag tgaactcaga tacgcccgct
    17341 ggtctgaaat tgatattgac aatgccatgt ggactattcc agccgaacgc gaacccctgc
    17401 caggcgtaaa attctcacac cggggctcca agatgcgaac accacatctt gtgccactca
    17461 gcaaacaggc tgtagccata ctgacagaac tacagacatg ggctggtgaa aatggtctga
    17521 tctttacggg agcacatgac ccgcgtaaac caatcagtga aaatgtaatg aacgcatc
//
LOCUS       DADLTD010000075        17501 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLTD010000075 DADLTD010000000
VERSION     DADLTD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.075"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..744
                     /locus_tag="JN701_001994"
     CDS             <1..744
                     /locus_tag="JN701_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7364450.1"
                     /translation="SSTDKPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSD
                     WQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLL
                     QDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYI
                     DQLHTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRD
                     IRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(815..1264)
                     /gene="rplI"
                     /locus_tag="JN701_001995"
     CDS             complement(815..1264)
                     /gene="rplI"
                     /locus_tag="JN701_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY7364451.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="JN701_001996"
     CDS             complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="JN701_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY7364452.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1538..1852)
                     /gene="priB"
                     /locus_tag="JN701_001997"
     CDS             complement(1538..1852)
                     /gene="priB"
                     /locus_tag="JN701_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY7364453.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="JN701_001998"
     CDS             complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="JN701_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY7364454.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2581..2856
                     /locus_tag="JN701_001999"
     CDS             2581..2856
                     /locus_tag="JN701_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7364455.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2931..3482)
                     /locus_tag="JN701_002000"
     CDS             complement(2931..3482)
                     /locus_tag="JN701_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HAY7364456.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3579..4265)
                     /locus_tag="JN701_002001"
     CDS             complement(3579..4265)
                     /locus_tag="JN701_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7364457.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4265..5119)
                     /locus_tag="JN701_002002"
     CDS             complement(4265..5119)
                     /locus_tag="JN701_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY7364458.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNYPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="JN701_002003"
     CDS             complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="JN701_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY7364459.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="JN701_002004"
     CDS             complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="JN701_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY7364460.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="JN701_002005"
     CDS             complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="JN701_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY7364461.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="JN701_002006"
     CDS             complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="JN701_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY7364462.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8340..9404
                     /gene="ulaG"
                     /locus_tag="JN701_002007"
     CDS             8340..9404
                     /gene="ulaG"
                     /locus_tag="JN701_002007"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY7364463.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTPPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9512..10267
                     /gene="ulaR"
                     /locus_tag="JN701_002008"
     CDS             9512..10267
                     /gene="ulaR"
                     /locus_tag="JN701_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY7364464.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="JN701_002009"
     CDS             complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="JN701_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7364465.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11195..11524
                     /gene="bsmA"
                     /locus_tag="JN701_002010"
     CDS             11195..11524
                     /gene="bsmA"
                     /locus_tag="JN701_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY7364466.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11673..11948
                     /gene="yjfN"
                     /locus_tag="JN701_002011"
     CDS             11673..11948
                     /gene="yjfN"
                     /locus_tag="JN701_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY7364467.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12065..13690)
                     /locus_tag="JN701_002012"
     CDS             complement(12065..13690)
                     /locus_tag="JN701_002012"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY7364468.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPATKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13774..14832)
                     /locus_tag="JN701_002013"
     CDS             complement(13774..14832)
                     /locus_tag="JN701_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7364469.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14835..15473)
                     /locus_tag="JN701_002014"
     CDS             complement(14835..15473)
                     /locus_tag="JN701_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY7364470.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15483..15881)
                     /locus_tag="JN701_002015"
     CDS             complement(15483..15881)
                     /locus_tag="JN701_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY7364471.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15899..16558)
                     /locus_tag="JN701_002016"
     CDS             complement(15899..16558)
                     /locus_tag="JN701_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HAY7364472.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16609..17307)
                     /locus_tag="JN701_002017"
     CDS             complement(16609..17307)
                     /locus_tag="JN701_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HAY7364473.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17380..>17501)
                     /locus_tag="JN701_002018"
     CDS             complement(17380..>17501)
                     /locus_tag="JN701_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HAY7364474.1"
                     /translation="KMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4467 a         4673 c         4186 g         4175 t
ORIGIN      
        1 tcgtccaccg acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg
       61 tcaaatcgtg gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta
      121 gatctctctg actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga
      181 gtaacaatga atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt
      241 cacgcccaga aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat
      301 ttattgcaag atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt
      361 ttgggacctg gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc
      421 cttcctgccc aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc
      481 gaccaacttc acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg
      541 ctaaacgcgt attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat
      601 tcagcagcaa tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg
      661 agtgatcgta tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt
      721 gaatggatct acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa
      781 tggtcggcgt ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca
      841 ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca
      901 gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag
      961 cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag
     1021 acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca
     1081 gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt
     1141 tcttggtagc tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt
     1201 taacgtttac ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt
     1261 gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc
     1321 agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg
     1381 gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta
     1441 gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg
     1501 gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt
     1561 caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa
     1621 tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt
     1681 cgtgtccgct aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct
     1741 cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct
     1801 ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt
     1861 actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac
     1921 gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg
     1981 tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat
     2041 cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt
     2101 tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg
     2161 caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag
     2221 gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag
     2281 cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa
     2341 aaatgacgcg ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat
     2401 ctcatctttc gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag
     2461 tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatt attgattaaa
     2521 attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct
     2581 atgttcagtc gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca
     2641 gccattgaaa ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg
     2701 atttatatca atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag
     2761 acagatgcgc agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac
     2821 ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat
     2881 tgccatagtt tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt
     2941 gcgacttccg gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct
     3001 attatccaga accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat
     3061 tgagtgagcg gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc
     3121 acaataagcc ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca
     3181 atacaatacc ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg
     3241 ataatatgtt ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt
     3301 agaaagatat acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag
     3361 gggacttccg ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt
     3421 aacgcaacgc catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc
     3481 ataaaaacac ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat
     3541 tacatcccgc aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg
     3601 ttaggaccgt gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt
     3661 tgtgggttaa tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg
     3721 ttatgcaccg cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca
     3781 attcccggcg tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg
     3841 ttcagttcat actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg
     3901 ccaaagaagt agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc
     3961 catgcggtgg catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg
     4021 tagagttcaa gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg
     4081 ccgctcatat cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg
     4141 ggcttgatca ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag
     4201 gtcaccagcc catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt
     4261 tgcattatgc cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg
     4321 ctttcaccac ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg
     4381 gcccgcaata gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt
     4441 cgccaaatgg cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga
     4501 tatgcccgat tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc
     4561 cgatatctgg gtagagctgg aaccacgggt agttgagata gtgcgcgtaa cccagcgcct
     4621 tgctgatgga gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc
     4681 ggctcgccat ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat
     4741 tggcttcctg atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct
     4801 gggcgaactg gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt
     4861 cgctgcccaa cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg
     4921 tttcaacaat cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca
     4981 ggcgctcgtc agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca
     5041 gtcgcaggcg ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg
     5101 ggatttgttt ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga
     5161 actgacgcgc ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca
     5221 catgaattgg aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg
     5281 tgactttgaa gcccatatcg gaaagacgtt tgattgcagt gatatcggct tcaccccacg
     5341 ccacgccagc ggcctgcgcg tcacggctgc ggtgataaac cacctgctga atacctgcgt
     5401 cgcgccactg ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc
     5461 cgttaaactc ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa
     5521 tcacggtcac ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat
     5581 cggcaatttt ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa
     5641 ccgcacgcac gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt
     5701 cggcaatcag gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt
     5761 gcaacatcgg taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg
     5821 agatccagta cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct
     5881 tcaaacaggt tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc
     5941 gccatggtga tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt
     6001 ttcagcgtca ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc
     6061 attgccaggc ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc
     6121 gcctggtagt aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt
     6181 actgcgtcct gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc
     6241 gaatcacgta atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc
     6301 acttccagca gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg
     6361 tatttgttgc cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc
     6421 gcgccgctca actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg
     6481 tttgattggg ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg
     6541 ttgccacaca cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt
     6601 tctgccagtt gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata
     6661 taagccagtg caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc
     6721 tgcatcatcg gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg
     6781 gtgagtttca ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc
     6841 ttcagcgccg cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag
     6901 aagaacatcg ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg
     6961 ccaaccgcaa tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc
     7021 gcgaagctat agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg
     7081 gagataccgt taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg
     7141 atgaagatcg ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact
     7201 ttgcctgcca tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag
     7261 atggtcatca caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc
     7321 agtttgaggt cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca
     7381 atccatgatg caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc
     7441 tgagtcggct tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc
     7501 gtgcagataa tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc
     7561 cccgcctgct ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga
     7621 cgcagcagca cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag
     7681 ctataggcat cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt
     7741 gcgccgttaa tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg
     7801 aggatgccgg accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta
     7861 ataataacgc tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc
     7921 aacaacgggg cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga
     7981 tctcctgaag aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta
     8041 attttcaaaa gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata
     8101 attccatacg gatagagtgg gattcacaca acgaaacaat tatttattta aatttagtta
     8161 aattcattta gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca
     8221 acaacttccc tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag
     8281 gattaatcaa aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga
     8341 tgagtaaagt gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg
     8401 gtagctggct gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt
     8461 ggtggctagg ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg
     8521 atttctggtg cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc
     8581 agatgcagcg catggctggc gtgaaaaaac tgcagccaaa cctacgcacc cccccgtttg
     8641 ttcttgatcc gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg
     8701 atcatatcga cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat
     8761 tcattggacc gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt
     8821 gcatcgtggt caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg
     8881 atgctttcga ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac
     8941 tgccggacgg catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc
     9001 tgtatcacag cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc
     9061 agatcgacgt ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga
     9121 ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc
     9181 accacgatat ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga
     9241 tgaaaaaaga tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat
     9301 ttacctggcc actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt
     9361 gcttcactat tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg
     9421 gatagcgtgg agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat
     9481 ctaaaaaaat caaattttat cggaatagct catgactgaa gcacaaagac atcaaattct
     9541 cctggaaatg ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg
     9601 aatttcgcct gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa
     9661 aaaggtgcgc aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa
     9721 tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct
     9781 ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg
     9841 ggaaatgtgt ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct
     9901 gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc
     9961 catcacttta agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac
    10021 cagcggaaaa gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc
    10081 agagcagaag atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat
    10141 tggcgaacgc gcgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg
    10201 caaaaatgct aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg
    10261 tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg
    10321 gtgacgcacg cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa
    10381 ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca
    10441 cagcagtaga ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag
    10501 cggcgcgata atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg
    10561 aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt
    10621 caccgtgggg tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc
    10681 gactgccaga cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt
    10741 gaattcctgc atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc
    10801 gctactaaaa cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg
    10861 cgccagcgca acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa
    10921 aattacgcac ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat
    10981 atctgccagc tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac
    11041 atgaagaaat ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg
    11101 ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca
    11161 ttcccccaaa gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt
    11221 tatttaccgg tttgccagtt tattattggt gttgatgtta agtgcctgta gcgcactgca
    11281 aggtacgcca cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca
    11341 gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga
    11401 cgcattagca gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt
    11461 catggtggac gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa
    11521 ataaatcaga caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct
    11581 gtgcacaata aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc
    11641 aacatgtggg gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc
    11701 tgcaattagt gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg
    11761 actgcgtgac gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc
    11821 cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc
    11881 gcattattac catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt
    11941 atgcatgagt gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc
    12001 ggcctacaat tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat
    12061 acgcttacac acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg
    12121 acaaccgtac gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg
    12181 gagaagcata tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct
    12241 ctcgccccag ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat
    12301 caaaatagcg atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc
    12361 ccgcttgctt attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc
    12421 aaatactgtt taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac
    12481 caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca
    12541 caaattttgt cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc
    12601 ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca
    12661 tgcgacttaa gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc
    12721 gttgatgtgc atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca
    12781 gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt
    12841 cctgccccaa caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc
    12901 gattacccag cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca
    12961 aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg
    13021 catcgctttg cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt
    13081 ccagacgctc tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca
    13141 ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc
    13201 gatcgctcag cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca
    13261 atggcgtggc ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat
    13321 gtaacataaa acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg
    13381 caagattgtg cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa
    13441 aacgcacatc gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca
    13501 cattcgccag ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg
    13561 ctagaaaatc gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat
    13621 cagacaggta taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt
    13681 gccagtgcac tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg
    13741 gattcctgac agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa
    13801 acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc
    13861 atcatcgaca atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg
    13921 aaacgcctgg tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt
    13981 attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac
    14041 atagctttcg ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt
    14101 aggatgacca ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact
    14161 tttccataac ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc
    14221 acgcatcatc cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag
    14281 atcggtcagt acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc
    14341 ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt
    14401 gtcctgacag cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat
    14461 caggcgttcc tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc
    14521 aaaatccatc cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc
    14581 ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc
    14641 caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat
    14701 ctggcgcaga gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat
    14761 atgaaagtcg aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt
    14821 gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg
    14881 ccgcgcgaaa cggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt
    14941 ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgagccagac ggggcgggaa
    15001 gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc
    15061 cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag
    15121 caattttcgt acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa
    15181 aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca
    15241 atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta
    15301 tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat
    15361 ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg
    15421 atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc
    15481 cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata
    15541 aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt
    15601 ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag
    15661 tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat
    15721 gtaccgctga atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg
    15781 tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac accaataaaa
    15841 aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt
    15901 aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc
    15961 actctggctc gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag
    16021 tatcttcggt tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat
    16081 ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc
    16141 tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgca cccatcgttt
    16201 tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga
    16261 caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca
    16321 gaaattcatc gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc
    16381 ggctgacagc ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca
    16441 gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac
    16501 cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat
    16561 tttatcctta aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg
    16621 ttggcgttgc agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc
    16681 ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc
    16741 agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc
    16801 cgctgtcgaa acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg
    16861 ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg
    16921 cgctgtcgct gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac
    16981 ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat
    17041 caacgacgga ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt
    17101 gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac
    17161 gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat
    17221 gcgcacgccc tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag
    17281 cgtaaataaa ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc
    17341 ttctgtaatt tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat
    17401 atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg
    17461 cacgctggag attcctaccg atgacaacgg catcatcttc t
//
LOCUS       DADLTD010000076        17483 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLTD010000076 DADLTD010000000
VERSION     DADLTD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.076"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(34..2631)
                     /gene="topA"
                     /locus_tag="JN701_002019"
     CDS             complement(34..2631)
                     /gene="topA"
                     /locus_tag="JN701_002019"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY7364475.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3011..3262
                     /locus_tag="JN701_002020"
     CDS             3011..3262
                     /locus_tag="JN701_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY7364476.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3298..4347)
                     /gene="sohB"
                     /locus_tag="JN701_002021"
     CDS             complement(3298..4347)
                     /gene="sohB"
                     /locus_tag="JN701_002021"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY7364477.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4567..5325
                     /locus_tag="JN701_002022"
     CDS             4567..5325
                     /locus_tag="JN701_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY7364478.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5322..5913
                     /gene="cobO"
                     /locus_tag="JN701_002023"
                     /pseudo
     CDS             5322..5913
                     /gene="cobO"
                     /locus_tag="JN701_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5953..6825)
                     /gene="rluB"
                     /locus_tag="JN701_002024"
     CDS             complement(5953..6825)
                     /gene="rluB"
                     /locus_tag="JN701_002024"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY7364479.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNSG"
     gene            complement(7036..8931)
                     /locus_tag="JN701_002025"
     CDS             complement(7036..8931)
                     /locus_tag="JN701_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HAY7364480.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8959..9579)
                     /locus_tag="JN701_002026"
     CDS             complement(8959..9579)
                     /locus_tag="JN701_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY7364481.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9576..10457)
                     /gene="yciV"
                     /locus_tag="JN701_002027"
     CDS             complement(9576..10457)
                     /gene="yciV"
                     /locus_tag="JN701_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY7364482.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10595..10639
                     /gene="trpL"
                     /locus_tag="JN701_002028"
     CDS             10595..10639
                     /gene="trpL"
                     /locus_tag="JN701_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY7364483.1"
                     /translation="MKAIFVLKAWWRTS"
     gene            10731..12293
                     /gene="trpE"
                     /locus_tag="JN701_002029"
     CDS             10731..12293
                     /gene="trpE"
                     /locus_tag="JN701_002029"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY7364484.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLNSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12293..13888
                     /gene="trpD"
                     /locus_tag="JN701_002030"
     CDS             12293..13888
                     /gene="trpD"
                     /locus_tag="JN701_002030"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY7364485.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13892..15250
                     /gene="trpCF"
                     /locus_tag="JN701_002031"
     CDS             13892..15250
                     /gene="trpCF"
                     /locus_tag="JN701_002031"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY7364486.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGG
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            15262..16455
                     /gene="trpB"
                     /locus_tag="JN701_002032"
     CDS             15262..16455
                     /gene="trpB"
                     /locus_tag="JN701_002032"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY7364487.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16455..17261
                     /gene="trpA"
                     /locus_tag="JN701_002033"
     CDS             16455..17261
                     /gene="trpA"
                     /locus_tag="JN701_002033"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY7364488.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4222 a         4672 c         4345 g         4244 t
ORIGIN      
        1 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
       61 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
      121 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
      181 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
      241 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaaaacacc
      301 cgcagcgccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
      361 tggcaccgga tcttctttcg gtggtgccac ttcgccgtta cgtaaaatct tacgtgtgtt
      421 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
      481 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
      541 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
      601 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
      661 gcgcagcgcg ttggtttcag cgtcttcgcc ttccagtacg ttcagcactt cgttttccgg
      721 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcataac cagaacagcc
      781 aaggaatacc ccggtgctcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
      841 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
      901 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
      961 ttcgtgattt gccacctggt cgaggctgtt ttccatctgt gcggtgaagt cgtagttcat
     1021 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
     1081 gaaacgacga ttttctactc gcacgtagcc acgatcctga atggtcgaaa tgatcgacgc
     1141 ataggtagac ggacgaccga taccgcgttt ttccagctct ttaaccagcg atgcttcact
     1201 gaaacgggct ggcggcttgg taaagtgctg ggctggtgta agttcaacga gcgtcagagc
     1261 atcgccttta ttaactgctg gtaagatgcg atcttcatcg cctttacgca acgcaggcat
     1321 cacttttgtc cagccatcaa aacgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
     1381 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggctacgaa
     1441 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
     1501 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
     1561 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
     1621 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
     1681 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaatcc
     1741 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
     1801 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
     1861 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcgttct gatgagtcac
     1921 ctgtaacgcc aacgcttcac cagatggcgt ggtcgtgctg gcatcgactt cccagaactc
     1981 ttccggcacg aacgctttaa tttcacgctc acgctcgacc accaggcgaa ccgccaccga
     2041 ctgcacacga ccggcagaca gaccacgagc gatctttttc catagcagcg gcgaaaccat
     2101 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
     2161 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
     2221 cactcggcta tagcgcgcat catcaccccc aatcacttcc cgcaggtgcc atgcaatggc
     2281 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
     2341 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
     2401 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
     2461 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
     2521 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcactaccca gatacttgtt
     2581 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
     2641 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
     2701 ttaccgatat ggactcgttc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
     2761 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggaatttt
     2821 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
     2881 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
     2941 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttatttaa
     3001 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
     3061 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
     3121 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
     3181 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
     3241 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
     3301 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
     3361 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
     3421 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
     3481 gcctttctct accgcctgtt gtccgtacca gtgttcacct gttgccacct gttcaatatc
     3541 cagagacgga cgcatacgct tcacaaaatc tttaaacaac tgatgcgttt cgttcaactc
     3601 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaacgtca gagtacgctt
     3661 atactgcccg gcagtgtgca gttcgatatc aatatatttg cttttcagga agcggttaaa
     3721 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
     3781 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
     3841 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
     3901 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
     3961 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
     4021 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
     4081 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
     4141 tttttgctga tgtgagccca tcagcgccgc ggccagttct tctttcatct ccttatactg
     4201 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
     4261 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tcgccaaaaa
     4321 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
     4381 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
     4441 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
     4501 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
     4561 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
     4621 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
     4681 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
     4741 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
     4801 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
     4861 aatgccggat tgctcggcga tatttgccca atgagcgaac aaaatccgca ggtctggcag
     4921 gacgtcatgc aggtcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
     4981 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
     5041 cgagctaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
     5101 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
     5161 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
     5221 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
     5281 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
     5341 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcacag gccccaggat
     5401 gaacgcggta ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac cgcggcattt
     5461 ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca gtttattaaa
     5521 ggcacctggc ctaatggcga acgcaatctg ctggaaccac atggcgttga gtttcaggtg
     5581 atggcaacgg gctttacctg ggatacacaa aaccgcgagt ctgataccgc cgcctgccgc
     5641 gaagtctggc aacatgcaaa gcggatgctt gctgattcct cactggatat ggttttgctt
     5701 gatgaactga cgtatatggt ggcgtatgac tatttgccac tggaagaagt ggtgcaggcg
     5761 ttaaatgaac gtccacatca acagacggtg attatcacgg gtcgaggctg tcatcgggat
     5821 attcttgaac tggcggatac ggtaagtgag ctgcgcccca tcaaacatgc gtttgatgcc
     5881 ggtgttaagg cgcaaatagg gattgattat taatctgtaa aaatgccgga tatgacgtcc
     5941 ggcatacttt gattaaccgc tattacgccc gccagaacga cgaccgccgc tcacctgact
     6001 gtgacgtttc accgcacgac gaatctgatt agccttcatg cgacgacggt ctttttctac
     6061 agcgacttta gagctggttt ctggcggaag ctccaccagt tcgcgcagat agttagtctg
     6121 ggcgaggtcc agctccgtcc agccaccgcg tggcagacct tttggcagtg ggatatcacc
     6181 gtaacgaaca cggatcaggc ggctcacctg cacaccaacc gcttcccaca gacgacgaac
     6241 ttcacggtta cggccttcgg tcagagtcac gttgtaccac tggttgatcc cttcgccgcc
     6301 gctgaacttg atggttttaa aagctgccgg accatcttcc agttgcacgc cacgactcaa
     6361 atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt cacgttcaac
     6421 ttcacggctt ggatgcatta aacggtttgc cagttcacca tcggtggtga acagcagcag
     6481 accacaggtg ttaacgtcca gacgacctac ggcaatccag cgtgcgccac gcagctttgg
     6541 cagacggtca aacaccgtcg gacgcccttc cggatcgtta cgggtacaca actcaccttc
     6601 cggtttgtaa taggccagaa cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag
     6661 atgaccatcg atacgaattt tcagcccagg ggtaacttca acgcgatcgc cgagtttagc
     6721 aattttgcca tcaacactca cacgaccggc ttcaataata gattcgattt cacggcggga
     6781 gccgtggcca gcgcgggcca gcactttctg taatttttcg ctcatagagc ttccttttgg
     6841 tgtcgccttc ccaggcgtct gatatgcttt tgtgcaacag tgagtcagaa cttacatctg
     6901 actcactgat agtaaagtct gtttgaactc ttcaggcctt aagtaaacga ctcgacgttt
     6961 atcggcctag ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     7021 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc cgccagcacc
     7081 accaccggaa taacttcgcc ccaacaaact ggtattggcc ccgcaactta ggtgcgtctg
     7141 taatgagaat aaaacgccgt tacgggcaat ctctggagcg cgaatcgccg aagaaagcga
     7201 aggggcaatg cgtaacgccc attccctccc caaatttgcc gatatagccc aacctagcaa
     7261 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgt ccactgtgtg ttgctgcctc
     7321 tttacgcgca agatagttaa cgatagcttc accgcgggca taacgttgct gcccagtttg
     7381 ggtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggcaaacagc
     7441 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatatagag
     7501 agaatagaag gccacgcctg aacaaatcag tggtaaaaac aaagtgatga ttgcacgatt
     7561 aacgcaaggc ttgtcatcat cgcgggcaaa caaaagctca agggaacaaa ataaaaaagg
     7621 aataatccag cagatcataa taaacatatc gcctaccaga ctcattcctg cccaacctac
     7681 gggactcagg ttaccgtaga gaatgatcgc caatattgcc agtccacccc attgcaaaaa
     7741 cgtatcaggc cggtaaaaca aagacttttt acgcggcaga taaaatttct gcatccgtaa
     7801 aaatgccctg cgtaagactc tgttgcgtct ccccgtaaga acgaccttat cgcctttacg
     7861 aaataacgct tccagtaata actgctctgc gggcagtaat acctcattat ttctggtcgc
     7921 gccttcatta acacaaatca ggatttgctg gttcttatca tactgatctt ccagatgaat
     7981 ttttcccttc acgatcagat ttacgatatc ggcacagaaa cctttatcgt cgtaaaccag
     8041 cttcgcatcg agacgtaaca tgccgggcgt gtaatctggc ggaatgacat cggtttcaat
     8101 cacatctacc ggcgtaaatt gcggcctgcg cttccagaga tataaccatc cacaaaccag
     8161 gaatagacac ggaaaccaaa ttaacaacga tgacgtggag ggtaaaagaa gatggctgaa
     8221 aatattcgtc gtttgtggag ccggggcatt gctaagtaac gcgtgaggcc agcggtagag
     8281 taccgtgaaa tcttcacgat aaaacggatc gcgggattca attcttccgt cctcaaggat
     8341 gcggccattt cgatatgtgt cgccctcttc tccagtaaag agatcgattt cgctaaatgg
     8401 attacccgcg atatcaggga acttgagtga aaatcgggtc ttatagattt caaacggcca
     8461 gtggttacca gtcacgttcc agattaacag atctgaatct ccctcacgca ggaaagcatt
     8521 tttaacctga taatgaattt gataatgata gtcgccttga ggcaagaaac gttgtttatc
     8581 gcccacgaca atactcatta acccgatatg ccagtcaaga tgccagggtt caggaatacc
     8641 atcacgagtg acgccaacaa tatgatagtt cagcagtgca gcgtccccat caggctgcat
     8701 ccagagtcgt ggtaaatcac gataaaatcc gtgcttaatc tgctgatttc gcgcctgaaa
     8761 atgaatattt tctttgatgt cgagcacccc atccggttgc agagtggctt caacatcaaa
     8821 gagcgagatc ccttcttcat aagaagggat ctccgggttt tctgcataac ttagagatga
     8881 tacaaacagc cctgctatca acagcaaaat gcagcgaaac ttccctgcca tagtcttcca
     8941 tccgtggaac gcaatttgtt ataagaaagg cttcacatca cccacgcctt cacgcaccac
     9001 gaccggcgta tcatcggtaa gatcaatgac cgttgtcggt ttctgtccga gatagccgcc
     9061 gtggataatc aaatccacct gtttttccag acggtcttta atttcttctg gatcggattc
     9121 ggtaaattcg ctgcctggca gcattagcga agtggaaagc atcggttcgc ccagtgcttc
     9181 aagcaacgcc tgcgcaatcg ggttagaagg cacacgcata ccgatggttt tgcgtttttc
     9241 ctgcaacaga cggcgtggca cctccttcgt ccccttcagg atgaaggtat agttgcccgg
     9301 cgtgttgttt ttcattaaac gaaacgcaac gttatccacg aatgaatagg tcgaaagttc
     9361 agaaagatcg cgacacatca gggtaaagtt gtgaccgtcc ggcagctggc gaatacgaca
     9421 aatacgctcc atcgcgtttt tatcttcaat tttacagccg agcgcatagc cggaatcagt
     9481 tggataaaca atcaccccgc ctttacgcac gatctccacc gcctggttga tcagacgttg
     9541 ctgcgggtta tcaggatgaa tataaaaaaa ctggctcata attccctctc tgtggtgttc
     9601 tgcggctgtt cccatagctg ccagacgcct tcaacgcctg cgggcagcca gagtttacga
     9661 cccagttcga tccacgggca tggctgatga aaatcagatc cttgtgatgc ccataaatga
     9721 tgctgacgcg caagtgtcgt caactgggtg cgttcattgg gcgattgctg gcactgcgcg
     9781 acttccatcg cgtcgccgtg gtgttcggca aaatgcgcaa ccaatctttt cagccattta
     9841 gcactaagat tgtaccgccc aggatgagct agcaccgcct taccgccaga atgatgaatg
     9901 acatcaatag cttgttctat tgtacaccac tgtggcggaa cgtatccggt tttcccgcgc
     9961 gccagatact ttttaaagac atctgccatt gaactggctt tgccgcactc aacaaggaaa
    10021 cgcgcaaaat gaccgcgcgt cactgcgccg ccctgcgcca gtcgttgtgc gccttccagc
    10081 gccccaggta tttgcgcttt ttcaagtctt tcggcaattt gctgcgcccg ctgattgcgc
    10141 cgttctgtct gctgcgcgag gaactcacac attagcggat gagtaatatc aatattcagc
    10201 ccgacaatat gaatttcatg attttcccag accgtggaaa tttccacgcc gggaataaga
    10261 ttcagcgcca gtcccgaacg tgaaatttcc tctcttgctg gcgcgattgc agctgtggtg
    10321 tcatggtcgg tgatcgccag ggtgccgacg cgcatctcga ctgcacggtg caccaatgct
    10381 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgcg
    10441 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
    10501 aacggttctg gcaaatattc tgaaatcagc tgttgacaat taatcatcga actagttaac
    10561 tagtacgcaa gttcacgtaa aaagggtatc gacaatgaaa gcaattttcg tactgaaagc
    10621 ttggtggcgc acttcctgaa acgggcagtg tattcaccat gcgtaaagca atcagatacc
    10681 cagcccgcct aatgagcggg cttttttttg aacaaaatta gagaataaca atgcaaacac
    10741 aaaaaccgac tctcgaactg ctaacctgcg aaggcgctta tcgcgacaac ccgactgcgc
    10801 tttttcacca gttgtgtggg gatcgtccgg caacgctgct gctggaatcc gcagatatcg
    10861 acagcaaaga tgatttaaaa agcctgctac tggtagacag tgcgctgcgc attacagcat
    10921 taggtgacac tgtcacaatt caggcacttt ccggcaacgg cgaagccctg ctgacactac
    10981 tggataacgc cctgcctgcg ggtgtggaaa atgaacaatc accaaactgc cgtgtgctgc
    11041 gcttcccccc tgtcagtcca ctgctggatg aagacgctcg cttatgctcc ctttcggttt
    11101 ttgacgcttt ccgtttattg cagaatctgt tgaatgtacc gaaggaagaa cgagaagcaa
    11161 tgttcttcgg cggcctgttc tcttatgacc ttgtggcggg atttgaagat ttaccgcaac
    11221 tgtcagcgga aaataactgc cctgatttct gtttttatct cgctgaaacg ctgatggtga
    11281 ttgaccatca gaaaaaaaac acccgcattc aggccagcct gtttgctccg aatgaagaag
    11341 aaaaacaacg tctcactgct cgcctgaacg atcttcgcca gcagctgacc gaagccgcgc
    11401 cgccgctgcc ggtggtttcc gtgccgcata tgcgttgtga atgtaaccag agcgatgaag
    11461 agttcggtgg cgtggtgcgt ttgttgcaaa aagcgattcg caccggagaa attttccagg
    11521 tggtgccgtc tcgccgtttc tctctgccct gcccgtcacc gctggcggcc tattacgtgc
    11581 tgaaaaagag taatcccagc ccgtacatgt tttttatgca ggataatgat ttcaccctgt
    11641 ttggcgcgtc gccggaaagt tcgctcaagt atgacgccac cagccgccag attgagatct
    11701 acccgattgc cggaacacgc ccgcgcggtc gtcgcgccga tggttcactg gacagagacc
    11761 tcgacagccg catcgaactg gaaatgcgta ccgatcataa agagctttct gaacatctga
    11821 tgctggtgga tctcgcccgt aatgatctgg cacgcatttg cacccccggc agccgctacg
    11881 tcgccgatct taccaaagtt gaccgttact cttacgtgat gcacctggtc tcccgcgtgg
    11941 tcggtgagct gcgccacgat ctcgacgccc tgcacgctta ccgcgcctgt atgaatatgg
    12001 ggacgttaag cggtgcgccg aaagtacgcg ccatgcagtt aattgccgag gcggaaggtc
    12061 gtcgccgcgg cagctacggc ggcgcggtag gttatttcac cgcgcatggc gatctcgaca
    12121 cctgcattgt gatccgctcg gcgctggtgg aaaacggtat cgccaccgtg caagccggtg
    12181 ctggcatagt ccttaattct gttccgcagt cggaagccga cgaaacccgt aataaagccc
    12241 gcgctgtact gcgcgctatt gccaccgcgc atcatgcaca ggagactttc tgatggctga
    12301 cattctgctg ctcgataata tcgactcttt tacgtacaac ctggcagatc agttgcgcag
    12361 caatgggcat aatgtggtga tttaccgcaa ccatattccg gcgcagacct taattgaacg
    12421 cctggcgacg atgagcaatc cggtgctgat gctttctcct ggccccggtg tgccgagcga
    12481 agccggttgt atgccggaac tcctcacccg cttgcgtggc aagctgccca ttattggcat
    12541 ttgcctcgga catcaggcga ttgtcgaagc ttacgggggc tatgtcggtc aggcgggcga
    12601 aattctccac ggtaaagcct ccagcattga acacgacggt caggcgatgt ttgccggatt
    12661 aacaaacccg ctgccagtgg cgcgttatca ctcgctggtt ggcagtaaca ttccggccgg
    12721 tttaaccatc aacgcccatt ttaatggcat ggtgatggca gtacgtcacg atgcggatcg
    12781 cgtttgtgga ttccagttcc atccggaatc cattcttact acccagggcg ctcgcctgct
    12841 ggaacaaacg ctggcctggg cgcagcggaa actagagcca accaacacgc tgcaaccgat
    12901 tctggaaaaa ctgtatcagg cgcagacgct tagccagcaa gaaagccacc agctgttttc
    12961 agcagtggta cgtggcgaac tgaaaccgga acaactggcg gcggcgctgg tgagcatgaa
    13021 aattcgcggc gagcacccga acgagatcgc cggagcagca accgcgttac tggaaaacgc
    13081 cgcgccgttc ccgcgcccgg attatctgtt tgccgatatc gtcggcaccg gtggtgacgg
    13141 cagtaacagc atcaatattt ccaccgccag tgcgtttgtc gccgcggcct gtgggctgaa
    13201 agtggcgaaa cacggcaacc gtagcgtctc cagtaaatct ggttcgtccg atctgctggc
    13261 ggcgttcggt attaatcttg atatgaatgc cgataaatcg cgccaagcgc tggatgagtt
    13321 aggcgtatgt ttcctctttg cgccgaaata tcacaccgga tttcgccatg cgatgccggt
    13381 tcgccagcaa ctgaaaaccc gcactctgtt caacgtgctg ggaccattga ttaacccggc
    13441 gcatccgccg ctggcgttaa ttggtgttta tagtccggaa ctggtgctgc cgattgccga
    13501 aaccttgcgc gtgctggggt atcaacgcgc ggcggtagtg cacagcggcg gtatggatga
    13561 agtttcatta cacgcgccga caatcgttgc cgaactgcat gacggtgaaa ttaagagcta
    13621 tcagctaact gcagaagatt ttggcctgac accctaccac caggagcaac tggcaggcgg
    13681 aacaccggaa gaaaaccgtg acattttaac acgcttgtta caaggtaaag gcgacgccgc
    13741 tcacgaagca gccgtcgcgg cgaatgtcgc catgttaatg cgcctgcatg gccatgaaga
    13801 tctgcaagcc aatgcgcaaa ccgttcttga ggtactgcgc agtggttccg cttacgacag
    13861 agttaccgca ctggcggcac gagggtaaat gatgcaaacc gttttagcga aaatcgtcgc
    13921 agacaaggcg atttgggtag aaacccgcaa acagcagcaa ccgctggcca gttttcagaa
    13981 tgaggttcag ccgagcacgc gacattttta tgatgcgcta cagggtgcac gcacggcgtt
    14041 tattctggag tgcaagaaag cgtcgccgtc aaaaggcgtg atccgtgatg atttcgatcc
    14101 ggcacgcatt gccgccattt ataaacatta cgcttcggca atttcggtgc tgactgatga
    14161 gaaatatttt caggggagct ttgatttcct ccccatcgtc agccaaatcg ccccgcagcc
    14221 gattttatgt aaagacttca ttatcgaccc ttaccagatc tatctggcgc gctattacca
    14281 ggccgatgcc tgcttattaa tgctttcagt actggatgac gaccaatatc gccagcttgc
    14341 agccgtcgct cacagtctgg agatgggggg gctgaccgaa gtcagtaatg aagaggaact
    14401 ggagcgcgcc attgcattag gggcaaaggt cgttggcatc aacaaccgcg atctgcgtga
    14461 tttgtcgatt gatctcaacc gtacccgcga gcttgcgccg aaactggggc acaacgtaac
    14521 ggtaatcagc gaatccggca tcaatactta cgctcaggtg cgcgagttaa gccacttcgc
    14581 taacggtttt ctgattggtt cggcgttgat ggcccatgac gatttgcacg ccgccgtgcg
    14641 ccgggtgttg ctgggtgaga ataaagtatg tggcctgacg cgtgggcaag atgctaaagc
    14701 agcttatgac gcgggcgcga tttacggtgg gttgattttt gtcgcgacat caccgcgttg
    14761 cgtcaacgtt gaacaggcgc aggaagtgat ggctgcggca ccgttgcagt atgttggcgt
    14821 gttccgcaat cacgatattg ccgatgtggt ggacaaagct aaggtgttat cgctggcggc
    14881 agtgcaactg catggtaatg aagatcagct gtatatcgat acgctgcgtg aagctctgcc
    14941 aacacatgtt gccatctgga aagcattaag cgtcggtgaa accctgcccg cccgcgagtt
    15001 tcagcacgtt gataaatatg ttttagacaa cggccagggt ggaagcgggc aacgttttga
    15061 ctggtcacta ttaaatggtc aatcgcttgg caacgttctg ctggcggggg gcttaggcgc
    15121 agataactgc gtggaagcgg cacaaaccgg ctgcgccgga cttgatttta attctgctgt
    15181 agagtcgcaa ccgggcatca aagacgcacg tcttttggcc tcggttttcc agacgctgcg
    15241 cgcatattaa ggaaaggaac aatgacaaca ttacttaacc cctattttgg tgagtttggc
    15301 ggcatgtacg tgccacaaat cctgatgcct gctctgcgcc agctggaaga agcttttgtc
    15361 agcgcgcaaa aagatcctga atttcaggct cagttcaacg acctgctgaa aaactatgcc
    15421 gggcgtccaa ccgcgctgac caaatgccag aacattacgg ctgggacgaa caccacgctg
    15481 tatctgaagc gcgaagattt gctgcacggc ggcgcgcata aaactaacca ggtgctcggt
    15541 caggctttac tggcgaagcg gatgggtaaa accgaaatca tcgccgaaac cggtgccggt
    15601 cagcatggcg tggcgtcggc ccttgccagc gccctgctcg gcctgaaatg ccgtatttat
    15661 atgggtgcca aagacgttga acgccagtcg cctaacgttt ttcgtatgcg cttaatgggt
    15721 gcggaagtga tcccggtaca tagcggttcc gcgacgctga aagatgcctg taacgaggcg
    15781 ctgcgcgact ggtccggcag ttatgaaacc gcgcactata tgctgggcac cgcagctggc
    15841 ccacatcctt atccgaccat tgtgtgtgag tttcagcgga tgattggcga agaaacgaaa
    15901 gcgcagattc tggaaagaga aggtcgcctg ccggatgccg ttatcgcctg tgttggcggc
    15961 ggttcgaatg ccatcggcat gtttgcagat ttcatcaacg aaaccaacgt cggcctgatt
    16021 ggtgtggagc cgggtggtca cggtatcgaa actggcgagc acggcgcacc gctaaaacat
    16081 ggtcgcgtgg gcatttattt cggtatgaaa gcgccgatga tgcaaaccga agacgggcag
    16141 attgaagaat cttactccat ctccgccgga ctggatttcc cgtctgtcgg cccacaacac
    16201 gcgtatctta acagcactgg acgcgctgat tacgtgtcta ttaccgatga tgaagccctt
    16261 gaagccttca aaacgctgtg cctgcacgaa gggattatcc cggcgctgga gtcttcccac
    16321 gccctggccc atgcgctgaa aatgatgcgc gaaaccccgg aaaaagagca gctactggtg
    16381 gttaaccttt ccggtcgcgg cgataaagac atcttcaccg ttcacgatat tttgaaagca
    16441 cgaggggaaa tctgatggaa cgctacgaat ctctgtttgc ccagttgaag gagcgcaaag
    16501 aaggcgcatt cgttcctttc gtcacgctca gggatccggg cattgagcag tcattgaaaa
    16561 ttatcgatac gctaattgaa gccggtgctg acgcgctgga gttaggtatc cccttctccg
    16621 acccactggc ggatggcccg accattcaaa acgccactct gcgcgccttt gcggcaggtg
    16681 tgactccggc acaatgtttt gaaatgctgg cactgattcg ccagaaacac ccgaccattc
    16741 ccattggcct gttgatgtat gccaatctgg tgtttaacaa aggcattgat gagttttatg
    16801 cccagtgcga gaaagtcggc gtcgattcgg tgctggttgc cgatgtgcca gttgaagagt
    16861 ccgcgccctt ccgccaggcc gcgttgcgcc ataatgtcgc acctatcttc atctgcccgc
    16921 caaatgccga tgacgacctg ctgcgccaga tagcctctta cggtcgtggt tacacctatt
    16981 tgctgtcacg agcgggcgtg accggcgcag aaaaccgcgc cgcgttaccc ctcaatcatc
    17041 tggttgcgaa gctgaaagag tacaacgctg cacctccatt gcaggggttt ggtatttccg
    17101 ccccggatca ggtaaaagca gcgattgatg caggagctgc gggcgcgatt tctggttcgg
    17161 ccattgttaa aatcatcgag caacatatta atgagccaga gaaaatgctg gcggcactga
    17221 aagtttttgt acaaccgatg aaagcggcga cgcgcagtta atcccacagc cgccagttcc
    17281 gttggcggca ttttaacttt ctttaatgaa gccggaaaaa tcctaaattc atttaatatt
    17341 tatcttttta ccgcttcgct tacccctgtc gaacgtcaac ttacgtcatt tttccgccca
    17401 acagtaatat aatcaaacaa attaatcccg caacataaca ccagtaaaat caataatttt
    17461 ctctaagtca cttattcctc aga
//
LOCUS       DADLTD010000077        17397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLTD010000077 DADLTD010000000
VERSION     DADLTD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.077"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            162..605
                     /locus_tag="JN701_002034"
     CDS             162..605
                     /locus_tag="JN701_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7364489.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            841..1659
                     /locus_tag="JN701_002035"
     CDS             841..1659
                     /locus_tag="JN701_002035"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY7364490.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1660..2718)
                     /gene="modC"
                     /locus_tag="JN701_002036"
     CDS             complement(1660..2718)
                     /gene="modC"
                     /locus_tag="JN701_002036"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY7364491.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2721..3410)
                     /gene="modB"
                     /locus_tag="JN701_002037"
     CDS             complement(2721..3410)
                     /gene="modB"
                     /locus_tag="JN701_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY7364492.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3410..4183)
                     /gene="modA"
                     /locus_tag="JN701_002038"
     CDS             complement(3410..4183)
                     /gene="modA"
                     /locus_tag="JN701_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7364493.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4349..4498)
                     /locus_tag="JN701_002039"
     CDS             complement(4349..4498)
                     /locus_tag="JN701_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY7364494.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4627..5415
                     /gene="modE"
                     /locus_tag="JN701_002040"
     CDS             4627..5415
                     /gene="modE"
                     /locus_tag="JN701_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY7364495.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5483..6955
                     /gene="modF"
                     /locus_tag="JN701_002041"
     CDS             5483..6955
                     /gene="modF"
                     /locus_tag="JN701_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY7364496.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7216..8232
                     /gene="galE"
                     /locus_tag="JN701_002042"
     CDS             7216..8232
                     /gene="galE"
                     /locus_tag="JN701_002042"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY7364497.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8242..9288
                     /gene="galT"
                     /locus_tag="JN701_002043"
     CDS             8242..9288
                     /gene="galT"
                     /locus_tag="JN701_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY7364498.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAYDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9292..10440
                     /gene="galK"
                     /locus_tag="JN701_002044"
     CDS             9292..10440
                     /gene="galK"
                     /locus_tag="JN701_002044"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY7364499.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10434..11474
                     /gene="galM"
                     /locus_tag="JN701_002045"
     CDS             10434..11474
                     /gene="galM"
                     /locus_tag="JN701_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY7364500.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11676..12428
                     /gene="gpmA"
                     /locus_tag="JN701_002046"
     CDS             11676..12428
                     /gene="gpmA"
                     /locus_tag="JN701_002046"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY7364501.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="JN701_002047"
     CDS             complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="JN701_002047"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY7364502.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13954..14334
                     /locus_tag="JN701_002048"
     CDS             13954..14334
                     /locus_tag="JN701_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364503.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14448..15389
                     /gene="zitB"
                     /locus_tag="JN701_002049"
     CDS             14448..15389
                     /gene="zitB"
                     /locus_tag="JN701_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY7364504.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15386..16105)
                     /locus_tag="JN701_002050"
     CDS             complement(15386..16105)
                     /locus_tag="JN701_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY7364505.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="JN701_002051"
     CDS             complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="JN701_002051"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY7364506.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4415 a         4529 c         4348 g         4105 t
ORIGIN      
        1 acatttttaa aacagctata gcttctggtc ttgccgaaga agatccagct tctgcaacca
       61 ggccattcag aactgaagtg aagcgcagtc gactgttaat tgacgagtat ctgttaatca
      121 ggaaaatagc agacgtccag aacgaatggt tcggcctttg catggatctt gcactggtaa
      181 caggacagcg cgaaggagat ctggcggcta tgagatggga ggatataaga gatggcagac
      241 tatacgtgga acaacaaaaa acaggggcaa aaattcgcat ttctctcccc accacgattt
      301 ccagattgaa tttaacactt accgatgtac tggataactt gaaaaaaata aacggtaaaa
      361 acgaaaagtt actcggaggg aagacagcca ggacaatcgc agcacagttc aggattgccc
      421 gagacacatc cggattgaaa tgggaaggtg acccaccgcc atttcatgaa atccgaagtt
      481 tatccggtcg cctgcactca gcagaaaaag gcagtgattt cactcaggcg ctccttggac
      541 atcgttcatc cagcatgacg gataaatacc gtgacggacg agggagggaa tggaaagaca
      601 tttagaatta atttcgaatg atttcgaaac accacttgta attaattgat tataaaggat
      661 taattcctaa gaggcatttg cttcatgaat gctcctttgc atttagcaac tgactgtaac
      721 agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat
      781 agtggtatcg gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc
      841 atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc
      901 ctgcttcctt catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc
      961 atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat
     1021 acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg
     1081 gaagcggacc caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac
     1141 cacattcacg gtctgatgta tgtggatgat gcaatggtct atgagcaccc gaccgggcat
     1201 gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca
     1261 caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg
     1321 acgcacgatg acatgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga
     1381 ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa
     1441 ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa cgtcgtggcg
     1501 ttcggcgata actttaatga tatcagtatg ctggaggccg ctggtacagg cgtggcgatg
     1561 ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
     1621 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
     1681 tttaatttgc gcgtacagcc acaggcctgg tttgatcgcc agttcatccc tggcccacgg
     1741 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctgaccgtt
     1801 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
     1861 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1921 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1981 gtaatgcgga tgatgctcaa gcaccgtcac tttcagaatg ctactttgtt gctctttcgg
     2041 cagccacgga ttcatcacgc tactacccca gacttcttcc agtgcgccaa aggctttcac
     2101 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     2161 gctgacatac aacatcggga tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag
     2221 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattctgg
     2281 tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg acaggctgcc
     2341 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     2401 gaccatgctt ttcgccatgc cgtagcgcag attgccacgc actttgtaat gcggaaacag
     2461 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc agataccttt
     2521 ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag
     2581 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     2641 gatgccattg gcgggcagcg tttcattaat agtcaggcaa tggttgccca acgtctggga
     2701 aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     2761 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     2821 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     2881 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2941 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     3001 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     3061 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     3121 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     3181 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     3241 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     3301 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     3361 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     3421 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     3481 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     3541 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     3601 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     3661 cttccgccgg agccaatttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     3721 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     3781 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     3841 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3901 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3961 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     4021 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     4081 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     4141 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     4201 aacgttatat aggtgacaat ataacggcta actcagaatt ttcccagcag ttattgctaa
     4261 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     4321 atgcccgact aagtgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     4381 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     4441 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     4501 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     4561 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     4621 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     4681 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     4741 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     4801 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     4861 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4921 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4981 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     5041 gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     5101 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     5161 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     5221 gccaacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     5281 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     5341 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     5401 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     5461 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     5521 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     5581 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     5641 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     5701 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     5761 tggcgaagat gacaccggac gcactacggc tgagatcatt caggatgaag taaaggatgc
     5821 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     5881 taaatacctt tccactggcg agacgcgaaa aatcctgctg tgtcaggcgc tgatgtcgga
     5941 gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca
     6001 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     6061 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     6121 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcaca
     6181 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     6241 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     6301 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     6361 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     6421 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     6481 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     6541 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     6601 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     6661 tacggctgac gctccgttcc atagtctttc ctggggacag cagcatctgg cgctgatcgt
     6721 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     6781 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     6841 gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6901 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6961 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     7021 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     7081 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     7141 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     7201 ctaatggagc gaattatgag agttctgctt accggtggta gcggttacat tggaagtcat
     7261 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     7321 agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt
     7381 gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca cgctatcgac
     7441 accgtgatcc acttcgccgg gctgaaagcc gttggcgaat cggtacaaaa accgctggaa
     7501 tattacgaca acaatgtcaa cggtactctg cgcctgatta gcgccatgcg cgccgctaac
     7561 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     7621 tacgttgaaa gcttcccgac cggcacaccg caaagccctt acggcaaaag caagctgatg
     7681 gtggaacaga tcctcaccga tctgcaaaaa gcccagccgg actggagcat tgccctgctg
     7741 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     7801 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     7861 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7921 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7981 gtacacatct acaacctcgg cgctggcata ggcagcagcg tgctggacgt ggttaatgcc
     8041 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgagggcgac
     8101 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     8161 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     8221 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     8281 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     8341 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgtacgatcc agattgcttc
     8401 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc acgattacac cgggacttac
     8461 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     8521 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     8581 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     8641 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     8701 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     8761 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     8821 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     8881 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8941 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     9001 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     9061 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     9121 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     9181 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     9241 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     9301 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     9361 caggcgcctg gccgcgtgaa tttgattggt gaacacacgg actacaacga cggtttcgtt
     9421 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     9481 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     9541 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     9601 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     9661 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     9721 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     9781 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     9841 gatcatgcct tgctgattga ctgtcgttca ctggggacca aagcagtttc catgccgaaa
     9901 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9961 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agccctgcgc
    10021 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
    10081 cgcgtgcggc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
    10141 cagggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
    10201 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agctgtgatt
    10261 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgttg
    10321 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
    10381 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
    10441 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
    10501 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gcccgtattc
    10561 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
    10621 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
    10681 gccgttatac ctttgacggt gaaaccgtga ccctttcgcc aagtcagggc gttaaccagc
    10741 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
    10801 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
    10861 gcgcaacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10921 cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10981 agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat ctgccggttg
    11041 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacgtct tttgatttcc
    11101 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    11161 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    11221 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg gctctgcaat
    11281 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aacagaacct tacgccgact
    11341 ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    11401 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    11461 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atctcatatt gctcaaatca
    11521 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    11581 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cgcggcaata taatgagaat
    11641 tattatcatt aaaagatgat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    11701 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    11761 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    11821 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    11881 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11941 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg
    12001 acgagcaggt gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag
    12061 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagaactgc
    12121 cgctgacgga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    12181 ttctaccgcg tatgaagagc ggtgagcgcg taatcatcgc cgcacacggt aactccctgc
    12241 gtgcgctggt gaaatatctt gataacatga gcgaagaaga gattcttgag ctgaatatcc
    12301 cgactggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    12361 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgcaaac cagggtaaag
    12421 cgaagtaaac gtcattcgtt taaaatgaga aagccgactg caagtgagtc ggcttttttg
    12481 ttgctaacaa tggagcacat tgcctgatgc gacgcttcgc gacttatcag gcctgtggtg
    12541 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    12601 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc cgatgcaggc
    12661 atcggtgatg ctcttaccgt aggccagcgg ctccccgctc tcgaggctct gattgccttc
    12721 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    12781 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    12841 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    12901 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcgcc
    12961 gttaccgctg gtattcacaa tcgccgaatg cccccatttc gttacggaca ggaagcagtg
    13021 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt cggtgccatt
    13081 tttgaagccg actggacaag aaagccctga tgccagttcg cggtgcacct gcgattcggt
    13141 ggtacgtgcg ccaattgcgc cccagctcat caggtcagcg agatattgtg gggtgatcat
    13201 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    13261 ggctatacga agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcag
    13321 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    13381 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    13441 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    13501 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagtagcggg
    13561 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    13621 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    13681 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacattat gaacggatta
    13741 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    13801 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    13861 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13921 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13981 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    14041 caaactaata acggccaggc aaacgccgca actgatgcgg gccaggtagc ccctgacgcc
    14101 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    14161 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    14221 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaacc
    14281 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    14341 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    14401 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    14461 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    14521 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    14581 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    14641 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    14701 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    14761 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga
    14821 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    14881 gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt
    14941 tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg
    15001 ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    15061 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    15121 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    15181 ggatggtagg tgagaagccg gtgatgacgc tgcatgtgca ggtgatcccg ccacacgctc
    15241 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcacg
    15301 ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg
    15361 tgtctggcca ttcacatcat caccattaat gggacagcgc gcatgagcct ctttcacgtg
    15421 cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    15481 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    15541 cccacaacag ccagttttcc acatacttac gcgtcatcag aagcattgcc acgatagata
    15601 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    15661 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    15721 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg
    15781 ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    15841 accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15901 catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga
    15961 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    16021 ctaccgcttc gatccatgag agatcataac cgcctgctcc tattggtata tgtaccagga
    16081 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    16141 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    16201 tctcgcagcc tttcatcaac atgaacctcg tgattgcttc cttcctgttc taatgcctct
    16261 gcgatggcct gaaggccatt catggccatc cacggacaat gcgcgcagct gcggcaggtt
    16321 gcaccctcac ctgcggttgg tgcttccagt aactctttat ctggcaccgc ctgctgcatt
    16381 ttgtagaaaa taccccgatc ggttgccaca ataagcctct gatgtggcaa tgttttcgca
    16441 gcagcgatca gttgactggt ggaaccgacc gcatccgcca tatcgacaat agcttgtggt
    16501 gattctggat gcaccagtat ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc
    16561 tgagtcttaa attcatcatg cacaatacag gcaccctgcc agcatagaat gtctccaccc
    16621 gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat tttttcaccc
    16681 aaactatcaa gatgatcaat aagttcgacg gcaatgcttg aagttaccac ccaatctgcg
    16741 cgcgctttta ccgcagcaga agtgttggcg tagacgacga cagtacgatc gggatgggca
    16801 tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat ccagtgaaca ttcagcctga
    16861 agtgtcggca tcagaattgt tttttccgga ctgagaattt tggcggtttc tcccataaat
    16921 ctcaccccag cgactaacaa agtagaagcg ggatgctttg caccgaagcg cgccatttcc
    16981 agagaatcag aaatacagcc accggtttct tctgccagtt gttgaatttc gggatcggta
    17041 tagtagtggg caaccatcac cgcattacgt tcttttagca gacgttttat cttctcgcgg
    17101 taatacgctt tttcatcaat gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc
    17161 gtgtctggat caaacattac gctcatctta ccatctcgtt ttacaggctt aacgttaaaa
    17221 ccgacattag cgtaatattc gctgttatcg gtgtaatttg ttttatatgc taaacaagat
    17281 agtcgaatgg tggggaaaag tcactcgaat tacgcctaat tccaccgacg tagagttgct
    17341 tgcacagtaa gctctgacga tgtcatcagg tttacaaagt gaaattgagg atgcctg
//
LOCUS       DADLTD010000078        17373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLTD010000078 DADLTD010000000
VERSION     DADLTD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.078"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            42..118
                     /locus_tag="JN701_002052"
     tRNA            42..118
                     /locus_tag="JN701_002052"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Pro,seq:tgg)
     gene            265..1500
                     /locus_tag="JN701_002053"
     CDS             265..1500
                     /locus_tag="JN701_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7364507.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(2424..3620)
                     /gene="hemY"
                     /locus_tag="JN701_002054"
     CDS             complement(2424..3620)
                     /gene="hemY"
                     /locus_tag="JN701_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY7364508.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(3623..4828)
                     /gene="hemX"
                     /locus_tag="JN701_002055"
     CDS             complement(3623..4828)
                     /gene="hemX"
                     /locus_tag="JN701_002055"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7364509.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(4850..5590)
                     /gene="hemD"
                     /locus_tag="JN701_002056"
     CDS             complement(4850..5590)
                     /gene="hemD"
                     /locus_tag="JN701_002056"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY7364510.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(5587..6528)
                     /gene="hemC"
                     /locus_tag="JN701_002057"
     CDS             complement(5587..6528)
                     /gene="hemC"
                     /locus_tag="JN701_002057"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY7364511.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            6915..9461
                     /gene="cyaA"
                     /locus_tag="JN701_002058"
     CDS             6915..9461
                     /gene="cyaA"
                     /locus_tag="JN701_002058"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY7364512.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(9501..9821)
                     /gene="cyaY"
                     /locus_tag="JN701_002059"
     CDS             complement(9501..9821)
                     /gene="cyaY"
                     /locus_tag="JN701_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY7364513.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            10284..10478
                     /locus_tag="JN701_002060"
     CDS             10284..10478
                     /locus_tag="JN701_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7364514.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            10523..11347
                     /gene="dapF"
                     /locus_tag="JN701_002061"
     CDS             10523..11347
                     /gene="dapF"
                     /locus_tag="JN701_002061"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY7364515.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11344..12051
                     /locus_tag="JN701_002062"
     CDS             11344..12051
                     /locus_tag="JN701_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY7364516.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12048..12944
                     /gene="xerC"
                     /locus_tag="JN701_002063"
     CDS             12048..12944
                     /gene="xerC"
                     /locus_tag="JN701_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY7364517.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            12944..13660
                     /gene="yigB"
                     /locus_tag="JN701_002064"
     CDS             12944..13660
                     /gene="yigB"
                     /locus_tag="JN701_002064"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY7364518.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            13744..15906
                     /gene="uvrD"
                     /locus_tag="JN701_002065"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             13744..15906
                     /gene="uvrD"
                     /locus_tag="JN701_002065"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY7364519.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(15951..16853)
                     /locus_tag="JN701_002066"
     CDS             complement(15951..16853)
                     /locus_tag="JN701_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HAY7364520.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(16936..17367)
                     /locus_tag="JN701_002067"
     CDS             complement(16936..17367)
                     /locus_tag="JN701_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364521.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFA
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3983 a         4482 c         4657 g         4251 t
ORIGIN      
        1 gactttaaat aattgaacta aaaattcaaa aagcagtatt tcggcgagta gcgcagcttg
       61 gtagcgcaac tggtttggga ccagtgggtc ggaggttcga atcctctctc gccgaccaat
      121 tttgaacccc gcttcggcgg ggttttttgt tttctgtgca tttcgtcacc ctctcttcgc
      181 aataaacgcc cgtaataact cattgcccca cggtatgatt tcgcccttaa cgtattgaag
      241 gatgacttca ggcaaggagt gaccatgctg caacaggttc caacgcgtgc ttttcatgtg
      301 atggcgaaac cgagtggttc cgattgtaat ctgaactgtg actactgttt ttatctcgaa
      361 aaacaatccc tttaccgcga aaagccagtc acgcatatgg acgatgacac gctggaagcg
      421 tatgtccgtc actatatcgc tgccagcgaa ccgcaaaacg aagtggcttt tacctggcag
      481 ggcggcgaac caacgttact cgggctggat ttttaccgcc gtgccgtaaa gttacaggcg
      541 aaatacggtg ctggcaggaa gataagtaac agcttccaga ctaacggcgt gctgctcgat
      601 gataaatggt gtgcatttct ggcagaaaat cattttcttg ttgggttatc gctggacggt
      661 ccggctgaga tccacaatca atatcgcgtg accaaaggtg gcagaccaac gcataagctg
      721 gtgatgcgtg ccctgacgct gctgcaaaaa catcatgtcg actataacgt gctggtctgc
      781 gtcaaccgca ccagcgcgca gcaaccgttg caggtttatg attttttgtg cgatgcggga
      841 gtcgaattca tccagtttat tccggtggtc gagcgcctgg ctgatgaaac agctgccagc
      901 gatggactga aactacatgc gcctggtgat attcaggggg aactgacgga atggtctgtg
      961 cgccccgatg aatttggtga atttctggtg gcgatttttg accactggat caaacgcgac
     1021 gtcggcaaga ttttcgtgat gaatatcgaa tgggcgtttg ccaattttgt cggtgcgccg
     1081 ggtgcggttt gccatcatca gccaacctgt gggcgctcgg tgattgttga gcacaacggc
     1141 gacgtttacg cctgcgatca ctatgtttat ccgcaatatc ggctggggaa tatgcatcag
     1201 caaacaattg cagaaatgat cgattccccg caacagcagg tgtttggtga agataaattt
     1261 aagcaattac cggcgcagtg tcgcagttgt aacgtgttaa aagcgtgctg gggaggctgc
     1321 ccgaaacacc gcttcatgct cgatgccagc ggcaaaccgg ggctgaatta tttgtgtgcc
     1381 gggtatcagc gttatttccg ccatctaccg ccatatctta aagcaatggc tgatttgctg
     1441 gcgcacggtc gcccggccag tgacattatg caggcacatt tgctggtggt gaataagtaa
     1501 aatagcggcc attttgtggc cgcctgcggt tgattgccgg atgcggcgta aacgccttat
     1561 ccggcctaca tgatcgtgca aattcaataa attgcagcgt tctgtggata ctggtttggg
     1621 tgatcgtatc cggcaaagcc tttgcgtgcg gtggcagttg acggggtatc tgctgcgctg
     1681 gccatgagag gaagagcggc ggcgacagca acaacaagaa gtttgggtga aaacgaaaat
     1741 tccatgcaaa atgctccggt ttcatgtcat caaaatgatg acgtaattaa gcattgataa
     1801 ttgagatccc tctccctgac aggatgatta cataaataat agtgacaaaa ataaattatt
     1861 tatttatcca gaaaatgaat tggaaaatca ggagagcgtt ttcaatccta cctctggcgc
     1921 agttgatatg taaggcaggt ttattatatc gcgttgatta ttgatgcttt ttttagtttt
     1981 aacggcaatt aatatatgtg ttattaattg aatgaatttt atcattcata ataagtatgt
     2041 gtaggatcaa gctcaggtta aatattcact caggaagtta ttactcagga agcaaagagg
     2101 attacagaat tatctcataa caagtgttaa gggatgttat ttcccgattc tctgtggcat
     2161 aataaacgag tagatgctca ttccatctct tatgttcgcc ttagtgcctc ataaactccg
     2221 gaatgacgca gagccgttta cggtgcttat cgtccactga cagatgtcgc ttatgcctca
     2281 tcagacacca tggacacaac gttgagtgaa gcacccactt gttgtcatac agacctgttt
     2341 taacgcctgc tccgtaataa gagcaggcgt ttttttatgc atcagaaagg tcccggaggt
     2401 gcttgcctcc gggtgagaag gaactactgt ggtgggttgt tctgcaacgt taacatcaaa
     2461 ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca gtctgtccag cgcgtcggca
     2521 agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg ctgcgcggaa ggcgagcgat
     2581 gcttcctgcc attctccgtg cttcatcagt gactggccca gtgtgctcca caacagcggg
     2641 cgatcgccga cgtttttgat ctgctggcgc agcacttttt ccagctgttc cggattgttg
     2701 gttttcagtc gaggaatcgg cagcagtagg cgatcgtcgt actggcgttt caggccatcg
     2761 atgataattt gctgggcagt atcatgatcg tcacattcaa taagatgttc cgccattgcc
     2821 acctgcaacg ctacttgatg acgcgttttc cggctttggt ttttccacca gttacgcaaa
     2881 ccttcactac cgttatcggc acgcgcctga tccatcaggc caatccatgc ctgttgttcc
     2941 agcattgcac gatgttcttc atcaccaaca tgggctttcg ccattgatgg gatgatatcc
     3001 agcagagaac tccatgcacc tgtgcggata tacgcctgtt ccgccagacg taatacttcc
     3061 ggatggcgtg gcgtaacttc cagcagctta tccacgccgt ggcgtgcagc atggttttca
     3121 ttacgggcca gttgcagacg tacacgggtg atttctaccg gaatggtgtc attgccggcc
     3181 agctccgctg cgcgttccag atgttggttg gcgcgcgctt catcaccacg ttgttgcgcg
     3241 gcttcggcag ccagtagata gttcaccacc ggttgttcag cgtgatcggc atttttcgcc
     3301 atcagctttt caacttgctg ataatcgcct tccgccagtt tcagcagcgc ctgttcggtc
     3361 tgcttacgtg cacggcgacg cttacgcccg acaaaccacc cacgggtgtg cgcgccagtg
     3421 cggaagatcc gccgcagtag ccactcaatg gcaaacagca ctaccatcgc cagaatcaat
     3481 atgatcgcca ggcccgtgac gctggtttcg atattgtagt tgtcggtctg gatcagcaca
     3541 taaccctgat gaccggcaat catcgggcca accactatcc ccgcaatcag caaaacaaag
     3601 agtaataaca cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcacctt
     3661 gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tttagcttcc gttgtccctg
     3721 ctgccggttg tgccagcagg ttacgcacgc gagtctgcat cagtttttcc agcatcgcct
     3781 ggctttgcag ggtttccgga agatccatcg agatattttg ctggcttaac tggtccacct
     3841 cgtcgaggaa cgctttggtg gtggcatcat cagtatcgta gtaagcacgt acccaggtgg
     3901 agacgttctc cagcgcctgg cgataagtct cttcctggtg acgcggtaca gcttgtgctg
     3961 cgaccagcag gcgagagcga atattttcgc gcagatagat atcctgattt ggcgctaaca
     4021 gcggtacggc ggtgtcatca cgacggcgaa tcgtaatgaa gttgtccata aagttctgcc
     4081 agcttttttg cagattgata cgccattcgc tgatggaact ggaaagctct tcaccatcgg
     4141 aatccatcgg cgaaccatcg ctgtcattat cggccagacg caggttatct acctgatttg
     4201 aaagctgatt aagcttaagg atgatgccgt cataatccac ctgcgaaact gcagaaaggc
     4261 tggcgatatc atcggtaatt gcccgacgaa cggtaatcag actcgggtca ttcatatccg
     4321 ccaggctggc atctgcactt ttcagcaacg ctgcagcggt cgtgacgtcc tgatcgctcc
     4381 acagcttccg tccggcgagt ttcaccagaa aatcggcctg agccagcagc caggttttag
     4441 catcgctgcc ggaaatggtg gcgacctttt gttggacttc atccaactgt tttgccagcg
     4501 tttcttgctg acgattcgcc tgcttaagct gtgcagcttg ttgcttaata atgccttcca
     4561 gctcggcttt ttggctctcc tgggcttttt gcaatgccgt cagttggtta gccagggcat
     4621 cgctggtggc ggtctgattg acggcctgtt gtttacccca gccatacaaa ccgacgcccg
     4681 ccgccagagc aatagcgata gccaccgcgc tgagaatcaa tgcggtattg ttcttactct
     4741 ttttttctgt tgcgacaggt tgtgacgtgg tgtccacggc ctccctggtc tcttcaacca
     4801 cggcggaggt tttttcttgt tccgtcatta tggcttcctg ttatgagagt tattgtaatg
     4861 cccgtaaaag cgcatcgttg tcagcgttat cggcgacctt aatgtcttgc cagcccagtt
     4921 cccgggcgag tttcgccaaa cgctcactga cgaccaatag tcgacagcgt agtaaccagt
     4981 gctcacgata ccattgtggg atcagcgacc agagttgctg caacatttca ccgctggtaa
     5041 caacgaccat cgtcacctcg cgggattgcc agcgcatcgc ttcttctgca ccatcgtaat
     5101 ggattgcgca tcgttgataa cattcacaaa aagtgacctc agcaccgcgc gctgtcaggg
     5161 tatccccaat tagctcacga ccgccattgc cacgtaatat cagcgcacgt ttgcccgcaa
     5221 tattttgtaa ttcaggtaat tgtagcaaga cttcgctgat ttcccgatcc tgcgggtaga
     5281 gaatcttctg tccgcttacg gtatgtagcg ccagtgcggt ggtgcgtcca atggcgaaat
     5341 aatcaggtag tcggggccat ttacgatctt gctgatgcag ctgtgattgg gcaaaagcaa
     5401 ccgcgtgttg cgagagggca aacaacagat cgctctcccc cagcgccgcc agttgatcag
     5461 caagttgcgg taattgtcga cccggagaaa actcaatcag cggaaaatgc caggccacct
     5521 gccccagtgt gcgcagacgg ctcactaact cttctccagc gggagacggg cgggtgacca
     5581 ggatactcat gccggggcgt ctccgttata gacttcagcg aggatctcgc gcgcgccgtt
     5641 attcagtagc tcttctgcca gcgaaatccc catttgttcg gcatcttgcg gcgcaccgcg
     5701 gcgttcaccg cgaataatct gcgaaccgtc cggcgcgccg accagcgcac gcagccagat
     5761 ttcaccatca ataagctcgg cgtagctacc aattggcacc tgacatccgc cttcgagacg
     5821 ggtattcatg gcgcgttctg cggtaacgcg cagtgcagtt tcgtggtgat tcagcgcggc
     5881 aagcagctcg cgagtgcgtg catcatcaag gcggcattca ataccgaccg caccttgtcc
     5941 taccgctgga agagaaatct cgggtggtaa cgcggcgcga atgcgggact ccagacctaa
     6001 acgttttagt ccggctacgg caagaatgat ggcatcgtat tcgccgttat ctagtttgct
     6061 caggcgagtg ccgacgttgc cgcgcaggga gcggataatc agatccgggc ggcgttcagc
     6121 cagttggcac tggcgacgta aactggacgt cccgacgata ctgcctgctg gtaacgcatc
     6181 cagactgtca tagttattgg acacaaaggc atcgcgagga tcttcacgct cacaaatagt
     6241 gaccagtccc agaccttgcg ggaattcaac cggcacatct ttcattgaat gtacggcgat
     6301 atcggcgcga ttttcgagga gcgcgacttc cagctctttt acaaataagc cttttccgcc
     6361 tactttcgcc agcggcgtat caagaatcac atcgccgcgc gtcaccatcg gtaccagttc
     6421 aacgaccagg cccggatggc tcgccatcaa cttgtctttg acatagtgtg cctgccagag
     6481 tgcaagtggg ctttggcgtg tggcaattct taaaacattg tctaacatgc ttgttaccgt
     6541 cattatcatc cgtggtccat cctaacatcc ttgccagagt gatgtcagtg ttgtggtgaa
     6601 acgtagacgc ctgcgcaaac cgtaaaatga ggtctggcag tggatcctga caggcgtttc
     6661 acgccgtcgt aataaggaat ttacagagaa taaacggtgc tacacttgta tgtagcgcat
     6721 ctttctttac ggtcaatcag caaggtgtta aattgatcac gttttagacc attttttcgt
     6781 cgtgaaacta aaaaaaccag gcgcgaaaag tggtaacggt tacctttgac atacgaaata
     6841 tcccgaatgc cgcgtgttac cgttgatgtt ggcggaatca cagtcatgac gggtagcaaa
     6901 tcaggcgata cgtcttgtac ctctatattg agactctgaa acagagactg gatgccataa
     6961 atcaattgcg tgtggatcgc gcgcttgctg ctatggggcc tgcattccaa caggtctaca
     7021 gtctactgcc gacattgttg cactatcacc atccgctaat gccgggttac cttgatggta
     7081 acgttcccaa aggcatttgc ctttacacgc ctgatgaaac tcaacgccac tacctgaacg
     7141 agcttgaact gtatcgtgga atgtcagtac aggatccacc gaaaggtgag cttccaatta
     7201 ctggtgtata caccatgggc agcacctcgt ccgtagggca aagttgttcc tctgacctgg
     7261 atatctgggt ctgtcatcaa tcttggctcg atagcgaaga gcgccagtta ctacaacgta
     7321 aatgtagtct gctggaaagc tgggccgcct cgctgggtgt ggaagtcagc ttcttcctga
     7381 ttgatgaaaa ccgcttccgt cataatgaaa gcggcagctt ggggggcgaa gattgtggct
     7441 ccacccagca tatactgctg cttgacgaat tttatcgtac cgccgtgcgt ctcgccggta
     7501 agcgtattct gtggaatatg gtgccgtgcg acgaagaaga gcattacgac gactatgtga
     7561 tgacgcttta cgcgcagggc gtgctgacgc caaatgaatg gctggatctc ggtggcttaa
     7621 gctcgctttc tgctgaagag tactttggtg ccagcctttg gcagctctac aagagtatcg
     7681 attccccata caaagcggta ctgaaaacac tgctgctgga agcctattcc tgggaatacc
     7741 cgaacccacg tctgctggcg aaagatatca aacagcgttt gcacgacggt gagattgtat
     7801 cgtttggtct cgatccatac tgcatgatgc tggagcgtgt tactgaatac ctgacggcga
     7861 ttgaagattt tacccgtctg gatttagtac gtcgctgctt ctatttaaaa gtgtgcgaaa
     7921 agctcagccg tgaacgcgcc tgcgtaggct ggcgtcgcgc agtgttgagc cagttagtga
     7981 gcgagtgggg ttgggacgaa gctcgtctgg caatgctcga taaccgcgct aactggaaga
     8041 ttgatcaggt gcgtgaggcg cacaacgagt tgctcgacgc gatgatgcag agctaccgta
     8101 atctgatccg ctttgcgcgt cgcaataacc ttagcgtctc cgccagtccg caggatatcg
     8161 gcgtgctgac gcgtaagctg tatgccgcgt ttgaagcatt accaggtaaa gtgacgctgg
     8221 taaacccgca gatttcaccc gatctctcgg aaccgaatct gacctttatt tatgtgccgc
     8281 cgggccgtgc taaccgttca ggttggtatc tgtataaccg cgcgccaaat attgagtcga
     8341 tcatcagcca tcagccgctg gaatataacc gttacctgaa caaactggtg gcgtgggcat
     8401 ggtttaacgg cctgctgacc tcgcgcaccc gtttgtatat taaaggtaac ggcattgtcg
     8461 atttgcctaa gttgcaggag atggtcgccg acgtgtcgca ccatttcccg ctgcgcttac
     8521 ctgcaccgac accgaaggcg ctctacagcc cgtgtgagat ccgccatctg gcgattatcg
     8581 ttaacctgga atatgacccg acagcggcgt tccgcaatca ggtggtgcat tttgatttcc
     8641 gtaagctgga tgtcttcagc tttggcgaga atcaaaattg cctggtaggt agcgttgacc
     8701 tgctgtaccg caactcgtgg aacgaagtgc gtacgctgca cttcaacggc gagcaatcga
     8761 tgatcgaagc cctgaaaact attctcggca aaatgcatca ggacgccgca ccgccagata
     8821 gcgtggaagt cttctgttat agccagcatc tgcgcgggtt aatccgtact cgcgtgcagc
     8881 aactggtttc tgagtgtatt gaactgcgcc tttccagcac ccgccaggaa accgggcgtt
     8941 tcaaggcgct gcgcgtttct ggtcaaacct ggggcttgtt cttcgaacgc ctgaatgtat
     9001 cggtacagaa gctggagaac gccatcgagt tttatggcgc tatttcgcat aacaaactgc
     9061 acggcctgtc agtgcaggtt gaaaccaatc acgtcaaatt accggcggtg gtggacggct
     9121 ttgctagtga agggatcatc cagttcttct tcgaagaaac gcaagacgag aatggcttta
     9181 atatctacat tcttgacgaa agcaaccggg ttgaggtgta tcaccactgc gaaggcagca
     9241 aagaggaact ggtgcgtgac gtcagtcgct tctactcgtc atcgcacgac cgcttcacct
     9301 acggctcaag cttcatcaac ttcaacctgc cgcagttcta tcagattgtg aaggttgatg
     9361 gtcgtgaaca ggtgattccg ttccgcacaa aatctatcgg taacatgccg cctgccaatc
     9421 aggatcacga tacgccgcta ttacagcaat atttttcgtg atgaacgtgc cggaaagcga
     9481 ggcttatccg gcatgcaatc ttagcggaaa ctgactgttt cacccgcctg ctgcgtcgcc
     9541 gcctgttcca gcaaatccca gaaggtttcg ccgctgcgat cgcaaatcca ctcatcgcct
     9601 ttcagatcaa aatggtagcc gccttgtttg gttgccagcc atacctggtg cagcggttcc
     9661 tggcggttga taatgatttt gctgccgttt tcaaaggtaa tggtcagcac gccgccgttg
     9721 atttcgcagt cgatatcgct gtcgccatcc cagtcgtcca gatgctcttc aatggtcagc
     9781 cacagttgat cagccaggcg atgaaattca ctgtcgttca ttgttgtatc ctgtttttaa
     9841 gtgatggcgg cagtatagcg gcatggggtc agggcttcaa agtttgcacc tctgctgctg
     9901 cgttccggca tgattcatcc gtcaccggaa taatgatgtc tctgtgtggc gaaagatttg
     9961 tctcttcatt agggcgcagt tacaccacgc ctttccctgt ttctggtaaa cattatgatc
    10021 aggtttaccg agcgagcatc ctcacgctga cggaactaaa aaagccaaca aacaaaccac
    10081 attgcgatag tgcataaagc catcctggcg cgaggtgccg atcacgaaac tgccagcaga
    10141 acataaatcc tcacgagtaa gcgttatact cgcagcattt cctcactttt cagacttcat
    10201 aaagagtcgc taaacgcttg cttttacgcc ttctcctgcg atgatagaaa gcagaaagcg
    10261 atgaacttta caggcaatcc ataatgaaaa acgtgtttaa ggtactcact gtattactta
    10321 ctctcttcag cctgacgggc tgcggtctga aaggtccgct ctatttcccg cctgcagata
    10381 aaaatgcacc gccgccgacc aaaccggtag agacgcaaac gcaatccacg gtgccggata
    10441 aaaacgatcg cgccactggg atggtccatc ccaggtgaat tactaaaagt cagtttctgt
    10501 acccgcgtga ttggagtaaa tgatgcagtt ctcgaaaatg catggccttg gcaacgattt
    10561 tatggtcgtc gacgcggtaa cgcagaatgt ctttttttca ccggagctga ttcgtcggct
    10621 ggcggatcgg cacctggggg tagggtttga ccagctgctg gtggttgagc cgccgtatga
    10681 tcctgaactg gattttcact atcgcatttt caatgctgat ggcagtgaag tggcgcagtg
    10741 cggcaacggt gcgcgctgct ttgcccgttt tgtgcgcctg aaagggctga ccaataagcg
    10801 cgatatccgc gtcagcaccg ccaacgggcg gatggttctg accgtcaccg atgatgatct
    10861 ggtccgcgta aatatgggcg aacccaactt cgaaccttcc gctgtgccgt ttcgcgctaa
    10921 caaagcggaa aagacctata ttatgcgcgc cgccgagcag acaatcttat gcggcgtggt
    10981 gtcgatggga aatccgcatt gcgtgattca ggtcgatgat gtcgataccg cggcggtaga
    11041 aacgcttggt cctgttctgg aaagccacga gcgttttccg gagcgcgcca atatcggttt
    11101 tatgcaagtg gttaagcgcg agcatattcg tttacgcgtt tatgagcgtg gggcaggaga
    11161 aacccaggcc tgcggcagcg gcgcgtgtgc ggcggttgca gtagggattc agcaaggttt
    11221 gctggccgaa gaagtacgcg tggaactccc cggcggtcgt cttgatatcg cctggaaagg
    11281 tccgggtcac ccgttatata tgactggccc ggcggtacat gtctacgacg gatttattca
    11341 tctatgaagc aaccagggga agaactgcag gaaacactca cggagcttga tgaccgggcg
    11401 gttgtcgatt atctgattaa aaatcctgag ttttttatcc gtaatgcgcg cgcagtagaa
    11461 gcgatacgtg tgccacatcc ggtacgcggc accgtttcgt tggtcgagtg gcacatggcc
    11521 cgcgcacgta atcatattca tgttctggaa gagaacatgg cgctgttgat ggaacaggct
    11581 atcgccaacg aaggcctgtt ttatcgccta ctctacctgc agcgcagtct caccgccgcc
    11641 agcagtctcg acgatatgct gatgcgcttt caccgctggg cgcgcgatct cggcctggca
    11701 ggtgcgagtc tgcgcctgtt tccggatcgc tggcgcttag gtgcgccgtc gaaccacact
    11761 catctggcat taagccgtca gtctttcgaa ccgctgcgta ttcagcgttt ggggcaggaa
    11821 cagcactatc ttgggccgct taacggacca gagctgctgg tggtgctacc ggaagcgaaa
    11881 gcggtgggat cggtggcgat gtcgatgctg ggaagcgatg ctgatttggg tgtcgtgctg
    11941 tttaccagtc gcgatgccag tcactatcaa caagggcaag gaacgcagtt acttcatgaa
    12001 attgcgctga tgttgccgga gcttctggag cgttggattg aacgcgtatg accgatttac
    12061 acaccgatgt agaacgctac ctacgttatc tgagcgtgga gcgccagctt agcccgataa
    12121 ccctgcttaa ctaccagcgt cagcttgagg cgatcatcaa ttttgccagc gaaaacggcc
    12181 tgcaaagctg gcagcaatgt gatgtgacga tggtgcgcaa ttttgctgta cgcagtcgcc
    12241 gtaaagggct gggagcagca agtctggcgt tacggctttc tgcgctacgt agcttttttg
    12301 actggctggt cagccagaac gaactcaaag ctaacccggc gaaaggtgtt tcggcaccga
    12361 aagcgccgcg tcatctgccg aaaaatatcg acgtcgacga tatgaatcgg ctgctggata
    12421 ttgatatcaa cgatcccctc gctgtacgcg accgtgcaat gctggaagtg atgtacggcg
    12481 ctggtctgcg tctttccgag ctggtggggc tggatatcaa acacctcgac ctggagtctg
    12541 gtgaagtgtg ggtaatgggg aaaggcagca aagagcgccg cctgccgatt ggtcgcaacg
    12601 ctgtggcgtg gattgagcac tggcttgatt tgcgcgacct gtttggtagc gaagacgacg
    12661 cgctttttct gtcgaaactg ggcaagcgta tctccgcgcg taatgtgcag aaacgctttg
    12721 ccgaatgggg cataaaacaa gggctgaata atcacgttca cccacataaa ttacgtcact
    12781 cgttcgctac gcatatgctg gagtcgagcg gcgatcttcg tggtgtgcag gagctactgg
    12841 gtcatgccaa cctctccacc acgcaaatct atactcatct tgattttcaa caccttgcct
    12901 cggtgtacga tgcggcgcat ccacgcgcca aacgggggaa ataatgcgtt tttaccggcc
    12961 tttggggcgc atctcggcgc tcacctttga cctggatgat accctttacg ataaccgtcc
    13021 ggtgattttg cgcaccgagc gagaggcgct tacctttgtg caaaattatc atccggcgct
    13081 gcgcagcttc cagaatgaag atctgcaacg cctgcgccag gcggtacggg aagcggaacc
    13141 cgagatttat cacgacgtga cgcgctggcg ttttcgttcg attgaacaag cgatgctcga
    13201 cgccgggctg agtgccgaag aagccagtgc aggcgcacac gcagcaatga tcaactttgc
    13261 caaatggcgc agccgaatcg acgtcccgca gcaaactcac gacaccttaa aacagctggc
    13321 gaagaaatgg ccgctggtgg cgatcaccaa cggtaacgcc cagccggaac tgtttggttt
    13381 gggggattat tttgagtttg tgctgcgcgc tggcccgcac gggcgctcaa aaccgttcag
    13441 cgatatgtac tttctggctg cggaaaaact caacgtgccg atcggcgaga tcttacatgt
    13501 tggggacgat ctcaccactg acgtgggtgg ggcaattcgc agcggaatgc aggcctgttg
    13561 gatcagaccg gaaaatggcg atctgatgca aacctgggac agccgtttac tgccgcatct
    13621 ggaaatttcc cggttggcat ctctgacctc gctgatataa tcagcaaatc tgtatatata
    13681 cccagctttt tggcggaggg cgttgcgctt ctccgcccaa cctattttta cgcggcggtg
    13741 ccaatggacg tttcttacct gctcgacagc cttaatgaca aacagcgcga agcggtggcc
    13801 gcgccacgca gcaaccttct ggtgctggcg ggcgcgggca gtggtaagac gcgcgtactg
    13861 gtgcatcgta tcgcctggct gatgagcgtg gaaaactgct tgccatactc gattatggcg
    13921 gtgacgttta ccaacaaagc ggcggcggag atgcgtcatc gtatcgggca gctaatgggc
    13981 accagccagg gcggcatgtg ggtcggcacc ttccacgggc tggcgcaccg tctgctgcgt
    14041 gcgcaccata tggacgccaa tctgccgcag gattttcaga tcctcgacag cgaggaccag
    14101 ctacgcctgc tcaaacgcct aatcaaggcg atgaacctcg acgagaagca gtggccgccg
    14161 cgccaggcaa tgtggtacat caacagccag aaagatgaag gcctgcgtcc acatcatatt
    14221 caaagctacg gtaatccggt ggagcagacc tggcagaagg tgtatcaggc gtatcaggaa
    14281 gcgtgtgacc gcgcgggcct ggtggacttc gccgagctgc tgctgcgcgc tcacgagttg
    14341 tggcttaaca agccgcatat cctgcaacac taccgcgaac gttttaccaa tatcctggtg
    14401 gacgaattcc aggataccaa caacattcag tacgcgtgga tccgcctgct ggcgggcgac
    14461 accggcaaag tgatgatcgt cggtgatgac gaccagtcaa tctacggctg gcgcggggcg
    14521 caggtggaga atattcagcg tttccttaat gatttccccg gtgccgaaac tattcgtctg
    14581 gagcaaaact accgctctac cagcaatatt ctgagcgccg ctaacgccct gattgaaaac
    14641 aataacgggc gtctgggtaa aaaactgtgg accgatggcg cggacggtga gcctatttcc
    14701 ctctattgcg cttttaacga actcgatgaa gcgcgttttg tggttaaccg catcaaaacc
    14761 tggcaggaca acggcggggc gcttgccgag tgcgccattc tctaccgcag taacgcccag
    14821 tcgcgtgtgc tggaagaagc actgttgcag gcgagtatgc cgtaccgtat ttacggcggg
    14881 atgcgcttct tcgaacgcca ggaaatcaaa gatgcgctct cgtatctgcg cctgattgcc
    14941 aaccgtaacg acgacgcggc ctttgagcgt gtggtgaata cgccaacgcg gggtattggt
    15001 gaccggacgc tggacgtggt acgtcagaca tcgcgcgatc gccagttaac actctggcag
    15061 gcatgtcgtg aactgttgca ggaaaaagcc ctcgccggac gtgctgccag cgccttacag
    15121 cggtttatgg aactgatcga cgccttagcg caggaaactg ccgatatgcc gctgcatgta
    15181 cagactgacc gggtaattaa agactccggc ctgcgtacca tgtacgagca ggagaagggc
    15241 gaaaaaggtc agacgcgtat cgaaaactta gaggaactgg tgacggcaac gcgccagttc
    15301 agctacaacg aagaagacga agatttaatg ccgctgcagg cgttcctatc ccatgcggcg
    15361 ctggaagcag gcgaagggca ggcggatacc tggcaggatg cggtacaact gatgaccatg
    15421 cactcggcga aaggtctgga gttcccgcag gtgtttatcg tcggtatgga agagggcatg
    15481 ttcccaagcc agatgtcgct ggatgaaggc gggcgtctgg aagaagaacg ccgtctggcc
    15541 tacgttggcg taacccgcgc gatgcagaaa ctgacgctga cctacgcgga aactcgccgt
    15601 ctgtatggca aagaggttta ccatcgcccg tcgcgcttta ttggcgagct gccggaagag
    15661 tgtgtggaag aggtgcgcct gcgcgccacg gtaagccgcc cggtcagcca tcagcggatg
    15721 ggtacgccga tggtcgagaa cgacagcggc tacaagctcg gccagcgtgt acgccacgct
    15781 aagtttggtg aaggcaccat cgtcaatatg gaaggcagcg gtgaacatag ccgtttgcag
    15841 gtggcattcc agggccaggg tattaaatgg ctggtggcgg cttatgcccg gttggagacg
    15901 gtgtgacgtt gcgggatgcg gtgcgccaca ccgcattccg catcagcacc ttacttagcg
    15961 cacttcttca catcaatatt ccagtcagca ccagacgcaa cctgatgttt ctgcgcgaaa
    16021 ttatccatat tcagcgccac ggaaacattc aataagctgt tcagatacac catcggctgg
    16081 ccttccggca catcgccgaa gcttgcgaca tacggcattt tccctgcgta tttttgctgg
    16141 gaaccttcgg aaatggtcac acacaacgtg tcgttgagtt tgatttctgc ctgattcagc
    16201 aactcgtcgc tgatattgct ccagacgttg ccgtactgaa tatcgagaat cgggatattg
    16261 cccttcactt caccttttgt tgcttttgct ttctgataag aaagttcgac aacttttgct
    16321 ggaagctctg gccccacctg ttcgaacgta atcgccccgg aagccagacg tgcaccggtg
    16381 taggcataca cgtcacgacc gtggaaggta tatgactttt cagaaccttt caggcggtta
    16441 gctttttcgt cgatttcccg gacgctatca atacccagag tttgtgccac caaagtgagc
    16501 gtaccgttat ctggtgaaac gaagtattga ccgcttttgg ttttcagcac taccgattta
    16561 cggtcagtgc ctacgcccgg atcgaccacg ctaacaaaca ccgaaccttg cggccagtaa
    16621 ctggcagtct gatacaggcg ataagcgcct tcccagatgt tatacggtgg aatttcgtgt
    16681 gttaaatcaa agattttaag attatggtca acgccgaatg cgaccccttt catcgccgag
    16741 acggcaccat ctttcaggct aaaatcagtt tgtaaaatca aagcgttatc cgcataggcg
    16801 gatgatgatc ccagacacgt taataaaaat aacgttgcga gcgttttttt catttttgtt
    16861 tcccttcatt tatctgatga ttttccagct gtgtagccag tcccagtttg tctggcctcc
    16921 tcgagtgtac aatgcttaac ctttccgctc gacggaaatc atggtgacaa aaggatagcg
    16981 ttgccacggg attgccccgc ctttcatata catatgtgaa attgtgccat caagataaag
    17041 caactgctca acgttcagtt tcgctttggc ataacaggca aaatcataaa aatttgtcgc
    17101 ctgctggctc aacaaaaaca cggcgttccc ttgtttatta atccctacac cgttacgaat
    17161 tttacgcgag gcaacattcg ggtgaatgcg tggattaatt acgccgtttt ctaataacat
    17221 tggccctgac tgcaccgcaa actgaatctc tttactggtt ttaaaggcat ccagacgaat
    17281 gatgccgact ttatctcccg caacataaaa cacgccgcca ggacggataa agaaattccc
    17341 ttcacctgaa gcgagattta acgccacctt ctg
//
LOCUS       DADLTD010000079        17150 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLTD010000079 DADLTD010000000
VERSION     DADLTD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.079"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..216)
                     /locus_tag="JN701_002068"
     CDS             complement(<1..216)
                     /locus_tag="JN701_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708138.2"
                     /note="catalyzes the of heptose(II) of the outer membrane
                     lipopolysaccharide core; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY7364522.1"
                     /translation="MKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNKTLARLAQQ
                     HPVVVLFRHAERCDRSTNQCLSDKTGIT"
     gene            548..1687
                     /gene="arnB"
                     /locus_tag="JN701_002069"
     CDS             548..1687
                     /gene="arnB"
                     /locus_tag="JN701_002069"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY7364523.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1691..2659
                     /gene="arnC"
                     /locus_tag="JN701_002070"
     CDS             1691..2659
                     /gene="arnC"
                     /locus_tag="JN701_002070"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY7364524.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2659..4641
                     /gene="arnA"
                     /locus_tag="JN701_002071"
     CDS             2659..4641
                     /gene="arnA"
                     /locus_tag="JN701_002071"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY7364525.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4638..5528
                     /locus_tag="JN701_002072"
     CDS             4638..5528
                     /locus_tag="JN701_002072"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY7364526.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5528..7180
                     /gene="arnT"
                     /locus_tag="JN701_002073"
     CDS             5528..7180
                     /gene="arnT"
                     /locus_tag="JN701_002073"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY7364527.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            7177..7512
                     /gene="arnE"
                     /locus_tag="JN701_002074"
     CDS             7177..7512
                     /gene="arnE"
                     /locus_tag="JN701_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY7364528.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7512..7898
                     /gene="arnF"
                     /locus_tag="JN701_002075"
     CDS             7512..7898
                     /gene="arnF"
                     /locus_tag="JN701_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY7364529.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7892..8158)
                     /gene="pmrD"
                     /locus_tag="JN701_002076"
     CDS             complement(7892..8158)
                     /gene="pmrD"
                     /locus_tag="JN701_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY7364530.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(8268..9623)
                     /gene="menE"
                     /locus_tag="JN701_002077"
     CDS             complement(8268..9623)
                     /gene="menE"
                     /locus_tag="JN701_002077"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY7364531.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9620..10582)
                     /gene="menC"
                     /locus_tag="JN701_002078"
     CDS             complement(9620..10582)
                     /gene="menC"
                     /locus_tag="JN701_002078"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY7364532.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10582..11439)
                     /gene="menB"
                     /locus_tag="JN701_002079"
     CDS             complement(10582..11439)
                     /gene="menB"
                     /locus_tag="JN701_002079"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY7364533.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11454..12212)
                     /gene="menH"
                     /locus_tag="JN701_002080"
     CDS             complement(11454..12212)
                     /gene="menH"
                     /locus_tag="JN701_002080"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY7364534.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(12209..13879)
                     /gene="menD"
                     /locus_tag="JN701_002081"
     CDS             complement(12209..13879)
                     /gene="menD"
                     /locus_tag="JN701_002081"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY7364535.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13968..15263)
                     /gene="menF"
                     /locus_tag="JN701_002082"
     CDS             complement(13968..15263)
                     /gene="menF"
                     /locus_tag="JN701_002082"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY7364536.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(15342..15647)
                     /gene="elaB"
                     /locus_tag="JN701_002083"
     CDS             complement(15342..15647)
                     /gene="elaB"
                     /locus_tag="JN701_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY7364537.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15702..16163)
                     /locus_tag="JN701_002084"
     CDS             complement(15702..16163)
                     /locus_tag="JN701_002084"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7364538.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            16228..17145
                     /gene="rbn"
                     /locus_tag="JN701_002085"
     CDS             16228..17145
                     /gene="rbn"
                     /locus_tag="JN701_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY7364539.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         4016 a         4620 c         4369 g         4145 t
ORIGIN      
        1 cgtaatacct gttttatctg acaagcattg attggttgaa cgatcgcaac gttcagcatg
       61 gcgaaacaaa acgacaaccg ggtgctgctg tgccagtctg gccaatgttt tattgtcgat
      121 acgtggcaaa ccattgctac tccaggcggc atgagtaccc agtccggcaa ttgcagcgag
      181 cgccagtaaa atgatgaaat atttttttga cttcaatgat gagcggcaaa aagctaactc
      241 gtaaactcca cctatagaca agcgcaacca gacaattacc gtgaaattga gcgacatttc
      301 tggcgataat tagcagttgg tgtaatatta aaaatcctac gatgtcggca aaatgcctca
      361 aaattttgcc aaatgcaaag cctaaataag aaaaaatata aaaatttcaa tatttacgtc
      421 taatattagt ttcttaaggt taagttaata ttctatcctt aaaatttcgt tccaaatggc
      481 aaaatataca caacactctt tatagcaaat ataagtggac aggtattcaa tggcggaagg
      541 aaaagcaatg tcagaatttt tgcctttttc gcgaccagca atgggcgtgg aggaactcgc
      601 tgcagttaaa gaagttctcg aatccggttg gatcacaacc ggtccgaaga atcaggcgct
      661 tgagcaagcc ttttgccagt tgacgggaaa tcagcatgcc atcgcggtca gttcagccac
      721 cgccggaatg catatcacgc taatggcgtt ggaaattggc aagggcgatg aagtgattac
      781 gccttccctg acctgggttt caaccctcaa tatgatttcc ttgttgggtg caacgccggt
      841 aatggtggat gtcgaccgcg atacgctgat ggtcacgcct gaagcgatcg agtcagccat
      901 tacgccacgc actaaagcca tcattccggt gcattatgcc ggtgcgccag cagatattga
      961 cgccattcgc gccattggcg aacgttacgg catcgcagtt atcgaagatg ctgcccatgc
     1021 cgtcggtacg tattacaaag ggcgacatat tggcgcaaaa ggtaccgcta ttttttcatt
     1081 tcatgccatt aaaaatatta cctgtgcgga aggtggcctg attgtaactg ataatgaaaa
     1141 ccttgcccgc cagctacgga tgctgaaatt tcacggcctg ggtgtcgatg cctatgacag
     1201 acaaacctgg ggccgagcac cgcaggctga agtcttaaca ccgggctata agtacaatct
     1261 gaccgatatt aacgccgcaa ttgccctgac acagttagca aaattagagc acctcaacac
     1321 ccgtcggcgc gaaattgccc agcaatatca gcaagcactg gctgctctcc cctttcagcc
     1381 attaagcctt cccgcctggc cgcacgttca cgcctggcat ctgtttatta ttcgtgtcga
     1441 tgaacaacgt tgtggtatca gtcgcgatgc gttgatggaa gcgttaaaag aaagaggcat
     1501 tggtaccggg ttacatttcc gcgccgctca cacacaaaaa tattatcgcg agcgttttcc
     1561 cacgctgtcg ttaccgaata ccgaatggaa tagcgaacgc atctgttctt tgccgctgtt
     1621 cccggatatg actaccgccg atgccgaccg tgtcatcaca gcccttcagc aactcgcagg
     1681 acaataagcc atgtttgaaa tccaccctgt taagaaagtc tcggtggtta ttcccgttta
     1741 taacgagcag gaaagcttac cggaattaat caggcgcacc accacagcct gtgaatcgtt
     1801 ggggaaagag tatgagatcc tgctgattga tgacggcagt agcgataatt ccgcgcatat
     1861 gctggtcgaa gcctcacaag cggagaacag ccatattgtg tctattttgc ttaaccgcaa
     1921 ttacgggcaa cattcagcga ttatggcggg attcagtcac gttactggcg acttaattat
     1981 tacccttgat gccgatctcc agaatccgcc agaagaaatc ccccgcctag tggcaaaagc
     2041 cgatgaaggt tacgacgtgg tagggactgt acgccagaac cgccaggaca gctggtttcg
     2101 taaaacagct tcgaagatga ttaaccggct tattcagcgc accaccggca aagcgatggg
     2161 tgactacggt tgtatgctgc gcgcctatcg ccgtcatatt gtcgatgcga tgttgcactg
     2221 ccatgaacgt agcaccttta tcccgattct ggcgaatatc ttcgcccgcc gtgccattga
     2281 aattccagta catcatgccg agcgtgagtt tggtgaatcc aaatacagtt ttatgcgcct
     2341 gattaatttg atgtacgacc tggtgacctg ccttaccaca acgccgctac gtatgctgag
     2401 tctgctcggc agcattattg cgattggagg ttttagcatt gcggtgctgc tggtgatttt
     2461 acgcctgacc ttcggaccac aatgggcggc agaaggcgtc tttatgctat ttgccgtgct
     2521 gtttactttt attggcgctc agtttatcgg catgggatta ctcggtgaat atatcggcag
     2581 gatctacacc gatgtccgcg cccgcccccg ctattttgtt cagcaagtta tccgtccatc
     2641 cagcaaggaa aatgaataat gaaaaccgtc gtttttgcct accacgatat gggatgcctc
     2701 ggtattgaag ctctgctggc tgccggttac gaaattagcg ccatttttac ccataccgat
     2761 aatcccggtg aaaaagcctt ttatggttcg gtggctcatc tggcggcgga aagaggcatt
     2821 ccggtttatg cgccggataa cgttaatcat ccgctgtggg tggaacgcat tgcccaactg
     2881 tcaccagagg tgattttctc tttttattat cgccatctta tttgcgacga gattttgcag
     2941 ctcgctcccg caggtgcatt taatctgcac ggttcgctgt taccaaaata tcgtggtcgc
     3001 gcgccgctga actgggtgct ggtgaacggt gaaacggaaa cgggcgttac attgcaccga
     3061 atggtaaaac gtgccgatgc tggggccatt gtggcgcaac tgcgcgttgc cattgcgcca
     3121 gacgatatcg ctattacgct gcatcataaa ttgtgccatg ccacgcgcca gctactggaa
     3181 cagacattac ccgccattaa acacggtaat attctggaaa tcgcccagcg cgaaaacgaa
     3241 gccacctgtt ttggtcgcag aacgccggat gacagtttcc ttgaatggca taaaccggca
     3301 tccgtactgc acaacatggt acgtgccgtt gccgatccgt ggccgggtgc cttcagctat
     3361 gttggcaatc agaaattcac cgtctggtcg tcgcgtgttc atcctcatgc cagcaaagca
     3421 cagccgggga gcgtgatttc tattgcaccg ctgttgattg cctgtggcga tggcgcgctg
     3481 gaaatcgtca ccggacaggc gggcgacggc attactatgc agggctcgca attagcgcag
     3541 acgctgggcc tggtgcaagg ttcacgcttg aatagccagc ctgcctgcac cgcccgacgc
     3601 cgtacccggg tactcatcct cggggtgaat ggctttattg gcaaccatct gacagaacgc
     3661 ctgctgcgcg aagatcatta tgaagtttac ggtctggata ttggcagcga tgcgataagc
     3721 cgttttctga atcatccgca ttttcacttt gttgaaggcg atatcagtat tcattccgaa
     3781 tggattgagt atcatgtcaa aaaatgtgat gtcgtcttgc cgctggtggc gatagccacg
     3841 ccgattgaat atacccgcaa cccgctgcgc gtatttgaac tcgattttga agagaatctg
     3901 cgcattatcc gctactgcgt gaagtaccgt aagcgaatca tcttcccgtc aacttcagaa
     3961 gtttatggga tgtgtagcga taaatacttc gatgaggacc attctaattt aatcgtcggc
     4021 ccggtgaata aaccacgctg gatttattcg gtatcaaaac aattacttga tcgggtgatc
     4081 tgggcctatg gcgaaaaaga gggtttacag ttcaccctct tccgcccgtt taactggatg
     4141 ggaccacgac tggataacct taatgcggca cgaatcggca gctcccgcgc tattacgcaa
     4201 ctcattctca atctggtaga aggttcaccg attaagctga ttgatggcgg aaaacaaaaa
     4261 cgctgcttta ctgatattcg cgatggtatc gaggcgttat accgcattat cgaaaatgcg
     4321 ggaaatcgct gcgacggtga aattatcaac attggcaatc ctgagaacga agcgagcatt
     4381 gaggaactgg gcgagatgct gctggcgagc ttcgaaaaac atccgctgcg ccatcatttc
     4441 ccaccgtttg cgggctttcg cgttgtcgaa agtagcagct actacggcaa aggatatcag
     4501 gacgtagagc atcgtaaacc gagcatccgc aatgcccacc gctgcctgga ctgggagccg
     4561 aaaattgata tgcaggaaac catcgacgaa acgctggatt tcttcctgcg caccgttgat
     4621 cttacggata aaccatcatg accaaagtag gcttacgcat tgatgtcgat acctttcgtg
     4681 gcacccgtga aggcgtgccg cgtctgctgg aaatcttgag taagcataat attcaggcca
     4741 gcattttttt cagcgtcggc ccggacaata tgggccgcca tctctggcga ctggtgaagc
     4801 cacagttttt gtggaagatg ctgcgctcaa acgcggcatc gctttatggc tgggatattt
     4861 tactggcagg tacggcctgg ccaggtaaag agattggtca tgccaatgcc gatatcattc
     4921 gtgaagcggc taaacatcac gaagtcggcc tgcacgcctg ggatcaccat gcctggcaag
     4981 cccgtagcgg taactgggat cggcaaacaa tgatcgacga tattgcacgc ggtcttcgca
     5041 ctctggaaga gattatcggt caaccggtaa cctgttctgc cgctgcgggc tggcgtgccg
     5101 accagaaggt gatcgaagca aaagaagcgt tccatttgcg ctacaacagc gattgtcgtg
     5161 gggccatgcc gttccgtcca ttgctcgaat caggaaaccc tggcactgcg caaattccgg
     5221 tgaccttacc cacctgggat gaagtgattg gtcgggatgt gaaaacagaa gattttaacg
     5281 gttggttact caaccgcatc ctgcgagata aaggcacgcc ggtttatacc attcatgcag
     5341 aagttgaagg ctgcgcttat cagcataatt ttgtggatct cctcaaacgc gcagctcagg
     5401 aaggcgtgac attttgccct ttaagcgaac tgttatcaga gacgttgccg ctcggacaag
     5461 ttgttcgcgg aaatattgcc ggacgtgaag gctggctggg ttgccaacaa attgcgggta
     5521 gtcgctgatg aaatcggtac gttaccttat cggcctcttc gcgtttattg cctgctatta
     5581 cctgttaccg atcagcacgc gtctgctctg gcaacccgat gaaacgcgtt atgcggaaat
     5641 cagtcgagaa atgctggcat ccggcgactg gattgtgccc catctgttag ggctacgtta
     5701 tttcgaaaaa cccattgccg gatactggat taacagcatt gggcaatggc tatttggcgc
     5761 gaataacttt ggtgtgcggg caggcgttat ctttgcgacc ctgttaactg ccgcgctggt
     5821 gacctggttt actctgcgct tatggcgcga taaacgtctg gctctactcg ccacagtaat
     5881 ttatctctca ttgtttattg tctatgccat cggcacttat gccgtgctcg atccgtttat
     5941 tgcattctgg ctggtggcgg gaatgtgcag cttctggctg gcaatgcagg cacagacgtg
     6001 gaaaggcaaa agcgcaggat ttttactgct gggaatcacc tgcggcatgg gggtgatgac
     6061 caaaggtttt ctcgcccttg ccgtgccggt attaagcgtg ctgccatggg tagcgacgca
     6121 aaaacgctgg aaagatctct ttatttacgg ctggctggcg gttatcagtt gcgtactgac
     6181 ggttctccct tggggactgg cgatagcgca gcgggagcct gacttctggc actatttttt
     6241 ctgggttgag catattcaac gctttgcact ggatgatgcc caacatagag ctccgttctg
     6301 gtactacgtg ccggtcatca ttgccggtag cctgccgtgg ctgggattac tccccggtgc
     6361 actgtacaca ggctggaaaa accgcaagca ttccgcaacc gtctatttgt tgagctggac
     6421 gataatgccg ctgctgtttt tctccgtcgc taaaggtaaa ttgcccacct atattctttc
     6481 ctgctttgca tctctggcaa tgctgatggc gcattacgct ttgctggcag caaaaaataa
     6541 tcctctggcg ctgcggatta atggctggat taacatcgct tttggcgtca ctggcattat
     6601 tgccacattt gtggtctccc cgtggggacc aatgaacacg ccggtgtggc aaaccttcga
     6661 gagctataaa gtcttttgtg cctggtcgat tttttcgcta tgggcatttt tcggctggta
     6721 caccttaaca aacgtcgaaa agacctggtc tttcgccgcg ctttgcccgc tggggctggc
     6781 gttgctggta ggattttcaa ttcctgacag agtcatggaa ggaaaacatc cgcaattttt
     6841 tgtcgagatg acacaagaat cacttcaacc aagccgctat attcttactg atagcgtcgg
     6901 tgttgccgca ggtctggcat ggagcctgca acgcgatgac atcatcatgt atcgccagac
     6961 aggtgagttg aaatacggcc ttaattatcc ggatgcgaaa gggagatttg tcagcggtga
     7021 tgagttcgca aactggctta atcaacatcg tcaggagggg attattacac tcgtgctttc
     7081 ggttgaccgc gatgaagata tcaacagtct cgccattccg cccgcagatg ccatcgatcg
     7141 tcaggagcgt ctggtgctga ttcagtatcg tcccaaatga tctggctaac attagtcttt
     7201 gccagcttgc ttagcgttgc cgggcagttg tgtcagaaac aggcaacctg ctttgtggcg
     7261 ataaacaaac ggcgcaaaca tatcgtgctg tggctgggac tggcgctggc ttgtcttggt
     7321 cttgccatgg tgctctggct gctggtcttg cagaacgtac cggtaggcat tgcctacccg
     7381 atgttaagtc tgaattttgt ctgggtgacg ctggctgcag taaaactgtg gcacgaaccg
     7441 gtatcgccgc gtcactggtg tggggtggcg ttcattattg gtggcattgt gatcctcggg
     7501 agtacggtgt aatgggcctg atatggggat tattcagcgt cattattgcc tcagttgcgc
     7561 aattaagcct gggttttgcg gcaagtcatc tgccgccgat gacgcactta tgggatttta
     7621 tcgcggctct gttagctttt ggcctggatg cccggatact gttactcggg ctgctgggtt
     7681 atctgctttc cgttttttgt tggtataaaa cgctacataa acttgcgcta agcaaagcct
     7741 atgccttgct cagtatgagt tatgtgctgg tatggattgc ttcaatggta cttcccggct
     7801 gggaagggac tttttcgctg aaagcactac tgggagtagc ctgtattatg agcgggttga
     7861 tgctgatttt cctgcccacg acaaaacaac gttactgagt tttccctgcc gctgtacact
     7921 gccgttccca ctcatcgggg ctataactgc ttgccgagag gactttcacc gtatgcagct
     7981 tttcacgatt aatacaataa agcgcattct gcaaaggcga gagtaaatcc cccactttca
     8041 cggcgaagtc actcttcact tcggcgatca tttttatcgc gccgccagca tcgcagagca
     8101 taagtacatg tctgttatct tgtttattgc agcacgattt tttgaccagc cattccattg
     8161 cattatcctg tttgctaaga gttttcacat caatttacag attaacagat aaactcttta
     8221 tcaacggcaa tggaatggca gaaatgagaa gtgaaacgga gggttaatta ttgctgacgt
     8281 tgcacccact cttttagcgc ctgacgtgaa attttaatac cgccgttttt cagctccggc
     8341 ggtaaagtca gccagcgcac cggttgttga aaacgagcca gcttatcttt cacccattcg
     8401 ccaagatcaa cggtttgctg gtcatactcc accaccgcta ccggccgatg accaaactcc
     8461 ttgtcggcaa cgggaacgat aaacacctgc aaaaccgcag gatgtgcagc aataacacgc
     8521 tcgacttctt ccggctgaat accctcaccg ccactgaata acagattgtc taaacgtccg
     8581 acaatggtca gcttgccatt atgcatctca ccgcgatcgc gcgtagcgta ccagccttcg
     8641 tcattaacca gtgaaaccag ttgcccgtta cgccagtaac cttctgccat actggcagcc
     8701 cgcagccaca cttcattatt aacgattttc acttcccgac ccggcagcgg cgaaccaacg
     8761 tctgccaggc cgtcggcttc tttcgcacat accgtggagg caaactcggt cagaccatag
     8821 ccgcaaaagc aacgaatccc ctgctcgcgc gcctgttccg tcaactcgac cgggatagcc
     8881 gcgccgccaa gtaacaccgc tttcagggaa acggaactgc ggttaaccag caaacgccag
     8941 agttgtgttg gcaccagtga agcgtgagta cagcctgcca gcatttgctc caatggctgt
     9001 ttatcacgca ccgtcatccg cgcaccagcg tataaccagc gccacataat tccctgaccg
     9061 gagacgtgaa acagcggtaa agagagcaac cagtcatcgt gatcgccaaa cggaatcagc
     9121 gataacacac cttgcgcact ggcaagatgg gcctgataag tatggacagc ggctttcggt
     9181 aaaccggtgg agccggaggt caatgtcatt gaacaaagac gtgttggttg ccacagagct
     9241 gaatgtgtgc cttcaaccat ctgaatatgc agtgaagtta acgccgggaa agtattttct
     9301 ccttccagca ccagcgcata ttgcagtgta agatttggca gtaattcctc aagcaacggt
     9361 tgcggaagct gtggatttac gggcagcacc cgcgccccgc attgcagtaa cgccagccag
     9421 gcgagcagcg tttgcggcgt attccacgcc cgcaacatca cgccgctgcc ctcaaccaca
     9481 ccctgcgccg caaagcccgt agctaactca tcgacgcgag cacaaagctc gcgccagttg
     9541 agttgctcgt cattaagacg taaggcgatg gcttctcccc gcacttgccg ccagtgacgc
     9601 cacggccagt cagagaagat cataacaacc gctccagtgc atcaacttcc acgacaggca
     9661 gcgtgctacc cggccagcga cgtacctgct gcgcctgcat cagatccagc gtgtccagcc
     9721 ctggaatggt gtccggcgtt aaccaggcgg caatccgcgc cagttgcgtt aagcctaagc
     9781 tcgactcaat agaagaactg atcaccgccg tcagcctcaa cgcgtgcgcc gcctgtacct
     9841 gctcgcgtac tttttccaga ctgcccgtga gcgtgggttt gataactacc gcgcgcacgc
     9901 cctcttcagc cacaaaggca aaatccggct cgcgcaggct ttcatcccag gcaatggcaa
     9961 tgccggtttc acgggcaaac gctcgcgaat catcgcgggt tttgcacggc tcttcgagaa
    10021 acgcgatgcg gtggcgataa tccgggttaa catatttggc aaactgctga cctttcagcg
    10081 gtgtccaggc gcgatttgcg tcaagacgca aatgcagatc cggaattgcc tccagcaaca
    10141 gattaaccac catgccgtcg cgcacggctt cgtacaatcc cactttgacc ttcgccactt
    10201 tctcgcctgg catatcagca agtttgagga tcagatcgtc aggatcgcca ttacacagtg
    10261 gtgccgcacg gtaattggcc gcctgcggga gcgtatctgc cagctccgcc aatgcacagc
    10321 ttacgccaaa ggcaacggaa ggcatctgca gtagctcgca atcgcctgcc agccagttat
    10381 ttacccaggc aagcagcacg ctttgcgcct cttcccaggt ttcctggctg aagcccggca
    10441 gtggggagat ctccccccac ccttcgcgct cgccctcacg caggcaaacg tacagcccgt
    10501 cgcgggtttt taaccgcctg tcgcgcagaa ccacccccgc gtccatgggg atctgccagc
    10561 ggtatacctg cgcgctacgc attacggatt ccgtttaaat ttgctgaaat caggctgacg
    10621 tttctggttg aaagcgttgc gaccttcctg accttcttcc gtcatgtaga acagcatggt
    10681 ggcgttgccc gccagctcct gcagccccgc ctgcccgtca cagtcggcgt tcagtgcagc
    10741 tttcaggcag cgcagcgcca tcggactgtt ttgcagcatt tcgcggcacc agcgaacggt
    10801 ctctttttcc agatccgcca gcggtaccac ggtgttgaca aggcccatat ccagcgcctg
    10861 tttcgcgtcg tactgacggc acaggaacca gatttcccgc gcttttttct gtccgacgat
    10921 gcgagccatg taggaagcgc cccagccgcc gtcgaaggaa ccgactttcg ggccagtctg
    10981 accgaagatg gcattatctg ccgcgatagt caggtcgcac atcatgtgca gaacgtgacc
    11041 gccgccgatg gagtagccag ccaccatcgc gacaaccggt ttcggacagg tacggatctg
    11101 acgctggaag tccagcacgt tcaggtgatg tacgccggaa tcatctttat agccgccgta
    11161 atcaccacgc actttctggt caccgccgga gcagaacgct ttatcacctg cgccagtcag
    11221 aatgatcacg ccgacgttgt cgtcataacg cgcatctgcc agtgcctgga tcatctcttt
    11281 taccgtcaga ggacggaagg cattgcgcac ctgcggacga ttaatggtga tttttgcgat
    11341 accatcggtg gatttttcat aacgaatgtc ctcgaaacct tcggagcagt cgtgccattc
    11401 aaccggtgcg taaagcattg cttcatcagg ataaatcata ttgtgtcctt tagtcagaaa
    11461 cgcaagatct gcgccagact tgcgattacg ccagcgggat tttcccgatg cgcgttatgt
    11521 ccggcgcgag gaatgacatg gcagtcggca gccagttccg ccgccagggc gcggaatttg
    11581 ctgtcacgtt caccacataa ataataaaac gcaaatgtgc gggcgctaag gttagcacgt
    11641 aaatcaggct ggacggcgag agaagtcgcc tccagcatgg cggcaagggt tgcgccatta
    11701 ttgttgctgc gcagcgccac cagctcccgg cgttgatcgt cattgagcga ggcaaaaaca
    11761 ggctgttgat accagtcggc aaataccgcc gttaacggtt ctgtgcgaaa acgctgcgcc
    11821 cattggcgat cggaacgctg acgttccgca cgttgttcag cattttgcag ccccggatgc
    11881 ccgccttcga caataacccc acaaagcccc gccagcccct ggcaagccgc catcatcgcc
    11941 acccgtccgc caagcgagta ccccaccagc cagaagtcaa ggatgttgta actaaccaag
    12001 gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc cgccgaacca
    12061 ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc gcccacttct
    12121 tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt
    12181 tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc agaagttgct
    12241 ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg
    12301 tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga cgatgatatt
    12361 tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac
    12421 gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca
    12481 ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt
    12541 aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc
    12601 ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg tacaccgggt
    12661 aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccgacaa
    12721 acaattgccc ctgttcaggc agatagtcgc tgatgcgatg cgccagttgc gcttcgccaa
    12781 acgcatcgcg acgggcaata accgcctgca ttgcctgttc cgccaggcgc gggatttcaa
    12841 cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca atattggcaa
    12901 ttaagcggcg tccacgatgg tgcgccggat caagccgccc ttcgatgtca tccacaatcc
    12961 agtactcttc gggttcacag cttgcctgcc attgcaggag ccgtttgccc gtcaggctgc
    13021 ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg gcattgccta
    13081 accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg ccaatcagcg
    13141 gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc
    13201 cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt
    13261 ccagactagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca cccagacgct
    13321 gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca aacgggcagt
    13381 tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc
    13441 aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagg
    13501 cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc tccggcgggc
    13561 gatcggcggt taagagaatc agtttttctc cggttaaccc ggcttcaatc agtgccggat
    13621 agagatttgc caccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt
    13681 tcgccagccc cagcgccaga tgccccaacc cacgctcatc gaaatgggtg tggtgaatga
    13741 atgcggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcct ggggcgatac
    13801 agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt
    13861 taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc
    13921 tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta ttccatttgt
    13981 aataaagtac gcaaccctgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg
    14041 gaaccacgga caatgcccgc accagcatat aatcgcacga cattgccgct aatttttgct
    14101 gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg
    14161 taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt
    14221 aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg
    14281 ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc
    14341 ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat atcttccacc
    14401 accagcatgt tctcgcgctg gtttttatca tccgccatca gccactcttc taactgctgc
    14461 gcctgcttat catcaggatg atttgctact gttcccgcca gcgcttcagt acgcagcgct
    14521 ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt ttcgccatca
    14581 aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc catcatcgcc
    14641 gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac cactttgtcg
    14701 agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca gcccgttttg
    14761 tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg cttgatactc
    14821 accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga gctttcgctg
    14881 aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag gcggggtaaa
    14941 agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa gtcggcgtgt
    15001 tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg
    15061 cccaggacgg cagcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt
    15121 tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatcg
    15181 ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc
    15241 agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg
    15301 ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac
    15361 agtcctagta ccagcccaac ggccgcgccc acaccaattc cttgccaagg tttttcgtgg
    15421 acgtagtcat cagcacgata aacagcctgc ttcgcccgat agtaataact gtctgaaacc
    15481 tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca
    15541 tatttctgat cagcgggatc gccagaggag cggagcacct cttccagtgt ctcactaagc
    15601 agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat
    15661 ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg aattacctcg
    15721 cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaaa
    15781 ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca
    15841 ggccagtgat gcgtgcaggt gtcgagggtt ttactcatca gttgctgccc cactttttcg
    15901 ccacgcaacg cttcactgac aatcacccga ccaataacga ccggctcaag atcatcatca
    15961 cttttcagaa tcctcgcata tgccaccagt tcatcatttt tccaaccgag aatatggcga
    16021 ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg ctcgacaaca
    16081 aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga
    16141 tgcagatctt gccattcaat cataatgctc tccttttaga tgtatacctt atactagccg
    16201 gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcagccggt
    16261 gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca tccgacccag
    16321 agcggacttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc
    16381 tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga tcatcttttt
    16441 ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc cttaacgatt
    16501 tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg
    16561 accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg
    16621 cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta tcgtattgaa
    16681 gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtgccgcct
    16741 ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga tggaaggcag
    16801 attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc tattttcggc
    16861 gataccggcc cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt catggtgcac
    16921 gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca tagctctaca
    16981 cgccaggctg cgacactagc ccgtgaggct ggagtcggta agctaatcat tacccacgtc
    17041 agctcgcgct atgatgacaa aggttgtcag cacctgttac gtgaatgcag gtcaattttc
    17101 ccggcgactg aactagcgaa tgatttcacc gtgtttaacg tttaactctg
//
LOCUS       DADLTD010000080        17104 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLTD010000080 DADLTD010000000
VERSION     DADLTD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.080"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..303)
                     /gene="hlyE"
                     /locus_tag="JN701_002086"
     CDS             complement(<1..303)
                     /gene="hlyE"
                     /locus_tag="JN701_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HAY7364540.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLF"
     gene            676..1095
                     /gene="umuD"
                     /locus_tag="JN701_002087"
     CDS             676..1095
                     /gene="umuD"
                     /locus_tag="JN701_002087"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7364541.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1095..2362
                     /locus_tag="JN701_002088"
                     /pseudo
     CDS             1095..2362
                     /locus_tag="JN701_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(2408..2938)
                     /gene="dsbB"
                     /locus_tag="JN701_002089"
     CDS             complement(2408..2938)
                     /gene="dsbB"
                     /locus_tag="JN701_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY7364542.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3084..4625)
                     /gene="nhaB"
                     /locus_tag="JN701_002090"
     CDS             complement(3084..4625)
                     /gene="nhaB"
                     /locus_tag="JN701_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY7364543.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKVAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4847..5566
                     /gene="fadR"
                     /locus_tag="JN701_002091"
     CDS             4847..5566
                     /gene="fadR"
                     /locus_tag="JN701_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY7364544.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5618..7150)
                     /locus_tag="JN701_002092"
     CDS             complement(5618..7150)
                     /locus_tag="JN701_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY7364545.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7480..8778
                     /locus_tag="JN701_002093"
     CDS             7480..8778
                     /locus_tag="JN701_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY7364546.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8846..9847
                     /gene="dadX"
                     /locus_tag="JN701_002094"
     CDS             8846..9847
                     /gene="dadX"
                     /locus_tag="JN701_002094"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY7364547.1"
                     /translation="MSARPRRTRVVKANAYGHGIERIWSALGATDGFALLNLEEAITL
                     RERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVN
                     SGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISSAMARIEQAAEGL
                     ECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEIIG
                     VQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTV
                     SMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV"
     gene            complement(10234..11970)
                     /locus_tag="JN701_002095"
     CDS             complement(10234..11970)
                     /locus_tag="JN701_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY7364548.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12065..12979)
                     /gene="ldcA"
                     /locus_tag="JN701_002096"
     CDS             complement(12065..12979)
                     /gene="ldcA"
                     /locus_tag="JN701_002096"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY7364549.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13079..13690
                     /gene="emtA"
                     /locus_tag="JN701_002097"
     CDS             13079..13690
                     /gene="emtA"
                     /locus_tag="JN701_002097"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY7364550.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13692..14426)
                     /locus_tag="JN701_002098"
     CDS             complement(13692..14426)
                     /locus_tag="JN701_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY7364551.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14627..14881
                     /locus_tag="JN701_002099"
     CDS             14627..14881
                     /locus_tag="JN701_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7364552.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15060..15500
                     /locus_tag="JN701_002100"
     CDS             15060..15500
                     /locus_tag="JN701_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364553.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIISLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15579..16924)
                     /gene="treA"
                     /locus_tag="JN701_002101"
                     /pseudo
     CDS             complement(15579..16924)
                     /gene="treA"
                     /locus_tag="JN701_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
BASE COUNT         4308 a         4224 c         4424 g         4148 t
ORIGIN      
        1 aaatagcaaa atatacgctg cgagcaattg cgtcgcaaca ccacaccatt catacactgt
       61 ttgggttgct tcaaaatact tatcctggct atccataagt aaggttttaa tatcgccgac
      121 taaaacggag gctgcctgtg aatactcctg tttaaagcga cttaactctt ttatggtttc
      181 atcaaaggtc tgccagggga tgacctgatc gagatattta ttataaagat ctaatgctcc
      241 atctgcggtt tcgattgcgt ttttaactac ttctaccatt ttatctgcaa cgatttcagt
      301 cataatcatt cgcctcttta aatatataaa ttgtaatgaa actcctgttt tacaactatt
      361 aataaatttt acttcatcta attcatagtt agccgggtgg gatgcgtcaa tgtctttatt
      421 tctattaata tgataaatat caaacaatgt ttaatgtcat tatggcgaat gcttctattc
      481 tatttttttg ccgggtgata tttttcattt ctgctggatg agcgtcgtcg ccagaaggcc
      541 acgtgagcac aggataagag aacgaaaaat cagcagtcta tacagcgaca aatattgata
      601 gcctgaatca gtattgatct gctggcaaga atagactact gtatataaaa acagtataac
      661 ttcaggcaga ttattatgtt gtttatcaag cctgcggatc tccgcgaaat tgtgactttt
      721 ccgctattta gcgatcttgt tcagtgtggc tttccttcac cggcagcaga ttacgtcgaa
      781 cagcgcatcg atctgaatca actgttgatc cagcatccca gcgcgactta cttcgtcaaa
      841 gcaagtggtg attctatgat tgatggtgga attagtgacg gtgatttact gattgtcgat
      901 agcgctatta ccgccagcca tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg
      961 gtgaaaaaat tgcaactacg cccgacggta cagcttattc ccatgaacag tgcgtactcg
     1021 cccattacca tcagtagcga agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt
     1081 aaggcgatgc gctgatgttt gctctctgtg atgtaaacgc gttttatgcc agctgtgaga
     1141 cggtgtttcg ccctgattta tggggtaaac cggtggttgt actatcgaat aatgacggtt
     1201 gcgttatcgc ccgaaacgct gaggcaaagg cgctgggtgt taaaatgggc gatccctggt
     1261 tcaaacaaaa agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc
     1321 tttacgcaga catgagcaat cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg
     1381 agatttacag tattgatgag gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc
     1441 tgactgattt tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg
     1501 gtgtggggat cgcccagacc aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat
     1561 ggcagcggca gacggggggg tggtggattt atcaaatctg gaacgccagc gtaaattaat
     1621 gtctgctctc cccgtggatg acgtctgggg gattggacgg cggatcagca aaaaactgga
     1681 cgcgatgggg atcaaaaccg ttcttgattt ggcggataca gatatccggt ttatccgtaa
     1741 acattttaat gtcgtgctcg aaagaacggt gcgtgaactg cgcggtgaac cctgtttgca
     1801 actggaagaa tttgcaccga caaagcagga aattatctgt tcccgctcgt ttggtgaacg
     1861 tatcacggat tatacgtcga tgcggcaggc catttgtagt tacgctgccc gggcggcgga
     1921 aaaacttcgc agtgagcatc aatattgtcg gtttatctcc acatttatta agacgtcacc
     1981 atttgcgctc aatgaacctt attacggcaa tagcgcgtcg gtaaaactgc tgacgcccac
     2041 tcaggacagc agggatatca ttaacgccgc tacgcgatct ctggatgcca tctggcaagc
     2101 gggccatcgc taccaaaaag cgggcgtgat gctgggggat ttcttcagtc agggagtcgc
     2161 gcagctcaat ttattcgatg acaacgcacc gcgccccggg agtgagcaat tgatgacggt
     2221 aatggataca ctgaatgcta aagagggcag aggaacactc tattttgccg ggcaggggat
     2281 ccaacaacaa tggcagatga agcgagccat gctttcacca cgttatacaa cgcgaagttc
     2341 tgatttactg cgggtcaaat aaatatagcg gcaggaaaaa agtgctcccg caggagcgcc
     2401 gaatggatta gcgaccgaac agatcacgtt ttttcgcttt aaacggctgg gaaatcatca
     2461 ccagcaccgc gacaatcagg taagcgataa aaataccgag cagccactgc ggcatttcca
     2521 gacctaaaaa atcccactga cgctcggcgc aatcgccaga ggcgacaaac acttgcggca
     2581 cccatttatc cagcggcagc cattccggga aacgaaccat aaaatcacag gtggcaaacg
     2641 gcgaaggata gagctgaagc atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac
     2701 tatacaacca gataaccatc gctacataac gcagcggagt tttcggggcg atcgcgccaa
     2761 tcagcgcagc acccagaacg ccgaataacg cgcagcgttc ataaatacag agcacgcaag
     2821 gtttcagtaa catcacatgc tggaaccaca gcgccgtcag ttccagtgcc agagcagtaa
     2881 acgccatcaa cagccacgcg ccccggcctt gtgaacattg gttcaaaaat cgcaacataa
     2941 tcatttccct gcaatatgca tagagtgcgc agtttaaacc aattcattcg ctgcgccacc
     3001 aggaggcagc aaaaatagga cgcaattgga caattatcgg gcgaaaaggc aaaccgggca
     3061 tttatatgcc cggtaagttg ttatcaaagc gttgctatcc agcccatttg cataaaccat
     3121 tcggttacag gggcaagcgt aaactcgacg cagagcagcc cgacgagtgt caggacgagg
     3181 gtgtaaggca gggccatcca caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt
     3241 gcagaggtca gcaggaacag gaacgcagcc tgaccgttcg gcgtagcgac agagggcaga
     3301 ttggtaccgg tattaatggc gaccgccagc agctcgtatt gcttcaacgt gatagcgcca
     3361 ctttccattg ccactttcgc ttcgttgata taaatcgtcc ccacgaagac gttatccgaa
     3421 atggatgaca gcagaccgtt gaaaatatag aacaacgaca gctgagcatg ttccgatgcc
     3481 tgcaacacaa actgaataat cggcgaaaac agttgttggt cgataatcac cgcgactacc
     3541 gagaaaaaca ccgtcaacag tgcggtgaat ggcagagatt cggtgaaggc tttaccgata
     3601 gcatgttcat cggtgacacc ggtcaatgat gtcgccagaa taatgaccga caaaccaatc
     3661 aagccaacct ccgccagatg caacgccagc gcagtcacca gccagacgcc gataatcgcc
     3721 tggacaatca gacggatttt atcctgacgg gtacgctggt ggcggctttg atcgtcaaac
     3781 tgttgcaaca cttcgcggac tttctccggc agcgtttcac cgtagccaaa ccaacgcagc
     3841 ttctctacca gcaggcaggt taacaggcca caaatcagta ccggaacggt caccggcgac
     3901 atgcgcagga agaaatcgcc aaaatgccag ccagccgctt tagcgatgat caggttctgt
     3961 ggttcgccca ccatggtcat tacgccgcct aatgcggtgc cgacaccggc atgcatcatc
     4021 aggctacgca gaaagccacg gaactgttcc agaaccactt tgtaatgctt gtcgatatga
     4081 ctatcgtctt gcaggtcggt atcttcagta cgggaagagg ctacgcggtg ataaatacca
     4141 taaaaaccga ctgcaacgct gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg
     4201 gagaggaacg ccgccgccac gcaaaaagag agcgacagca gcattttgga acgaatgctt
     4261 aacagcaaac gggtaaatat gaacagcaat agctgtttca taaaatagat acccgccacc
     4321 ataaacatca gtaacagcaa gacttcaaga tttgccgcca cctcttcacg gacgtgttcc
     4381 gcgctggtca tgccgatgaa taccgcttca atagccaaca gaccaccggg gagtagcggg
     4441 tagcatttca gggccatcgc cagagtgaaa ataaattccg ctaccagcaa ccagccagcg
     4501 acgaaagggc tgatgaggaa aattaacgga tttacgatta agaaaatgat gagggcgagt
     4561 ttgtaccagt cgggggactg gcccaaaaaa ttgcgccata gcgcgcggcc ccaggagatc
     4621 tccatgatgg tttcccttac cttacaaata atcaatgatg tttttatgtt taaacgcaaa
     4681 gcttaacggt caggcaggag tgaggcaagt cttgatagtc aaggggaaag agatgcggaa
     4741 aatgaagcct tgatcccttt tttcttcttt ttgtctgcta tcagcgtagt tagccctctg
     4801 gtatgatgag tccaactttg ttttgctgtg ttatggaaat ctcactatgg tcattaaggc
     4861 gcaaagcccg gcgggtttcg cggaagagta cattattgaa agtatctgga ataaccgctt
     4921 ccctcccggg actattttgc ccgcagaacg tgaactttca gaattaattg gcgtaacgcg
     4981 tactacgtta cgtgaagtgt tacagcgtct ggcacgagat ggctggttga ccattcaaca
     5041 tggcaagccg acgaaggtga ataatttctg ggaaacctct ggtttaaata tccttgaaac
     5101 actggcgcga ctggatcacg aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg
     5161 taccaatatt tccactattt ttattcgcac cgcgtttcgt cagcatcccg ataaagcgca
     5221 ggaagtgctg gctaccgcta atgaagtggc cgatcacgcc gatgcctttg ccgagctgga
     5281 ttacaacata ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg gtctgatcct
     5341 taacgggatg aaagggctgt atacgcgcat tggtcgtcac tatttcgcca atccggaagc
     5401 gcgcagtctg gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg aaggcgcgca
     5461 cgatcaggtg tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg
     5521 gatgcagaaa aatctgccgg gtgatttagc cattcagggg cgataatccc ttccgtttaa
     5581 aaagcaaacc cctcaaacga ggggtttttt gttgttttta cagatttccc attcttggcg
     5641 ggcaacgttc cagcaactcg acgctgccat cttcgttttg ctgttccagc atcacatcaa
     5701 atccccacag gcgatgcaca tgcttcagca cttctttgcg cccccgatcc agcggtgcgc
     5761 gattatgtgg aatgtaacgc agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga
     5821 tctgaatatt cggctccaga ttacttaagt tatattgcga cgataaccgg ttacggatct
     5881 cccgataacc ttcttcatta tgaatagcgg aaatctccag ataattatgc cgatcgtcat
     5941 ccagcacggt gaagaagcgg aaatcacgca tcactttcgg tgacagaaac tggctgataa
     6001 aactctcatc tttgaaatca cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg
     6061 cgatatccgg gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat
     6121 cctggaacat ggcgaacccg agggcatacg ggttgatgcc gctgtaccac gggctgttat
     6181 aggggggctg gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg
     6241 ttactttccc ttcatcatac agatggttaa ggatggtgta gtgccagaag gtggcccagc
     6301 cttcgttcat cacctgagtc tgtttttgcg gataaaaata ctggctcacc ttacgcacaa
     6361 tacgcaggat ttcacgctgc catgattcca gcagtggcgc atttttctcc ataaaataga
     6421 gcaggttttc ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct
     6481 cgcgcttcgg cagggtacgc cagagcatat tgacctgact ttgtagatac tcttcgcgac
     6541 ttttctgccg ggctttctct tcttgcagcg agattttttg cgggcgtttg taccggtcca
     6601 cgccgtagtt catcagcgcg tggcacgagt ccagaagccg ttctacttca tccacgccat
     6661 aacgttcttc gcactcggta atatatttac gggcaaaaat cagataatcg acaatcgaac
     6721 tggcgtcggt ccaactgcgg aataagtaat tgtttttaaa gaaagagtta tgcccatagc
     6781 aggcgtgtgc catcaccagc gcttgcatgg taatggtgtt ctcttccatc aggtaagcga
     6841 tacacgggtt agagttaatg acgatttcat aggccagtcc ttgctgaccg tgcttataca
     6901 gccgttcagt ctcgataaac tttttaccga atgaccagtg cggatagtta attggcatgc
     6961 cgacgctgga gtaggcatcc atcatctgtt ctgaggttat cacttcaatc tggtgcgggt
     7021 aggtatccag ccggtagagt ttcgccaccc ggtctatctc tgccagataa acatccagca
     7081 ggtcgaacgt ccagtcgggt ccatcgctca accgtgtggt gtccttattc atagaatcga
     7141 tcgtcgccat acgcgcacct cattgttgtc ggcgctctct gtgtggagca cctcatttca
     7201 agcatagaac acctgttaaa aaccgcgtcg ccggagaatt tttttctttg cgatttctta
     7261 ttatcagagt gccactaatc cgcttctgaa cggaatttta tgctggataa aaagggcgtt
     7321 cagcaggaga tactaaagac gccatattgc cgcagagtca gggagatgtg agccagctca
     7381 ccataaaaaa gctgtatgtt gaataatatt ttcaactgag ttatcaagat gtaattagat
     7441 tattattctt ttactgtatc taccgttatc ggagtggcta tgcgagttgt catactggga
     7501 agtggtgtgg taggcgttgc cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc
     7561 gtcattgatc gggagccggg ggccgccctg gaaaccagcg ccgcgaatgc cgggcaaatc
     7621 tcccccggat atgctgcacc gtgggcggca ccaggtgtgc ctttaaaagc gatcaaatgg
     7681 atgttccagc gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt tcagttgaaa
     7741 tggatgtggc aaatgttacg taactgcgac accagccact acatggaaaa caaagggcgg
     7801 atggtgcgtc tggcggaata cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat
     7861 attcagtatg aagggcgtca gggtgggacg ctgcaactgt tccgtaccga acaacagtat
     7921 gaaaatgcga cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg
     7981 gaatccagcc gcctggcgga agtggagcca gcgctggcag aagtagcgca caaactgacg
     8041 ggtggtctgc aactgcccaa tgatgaaacc ggagactgcc agctgtttac ccagaatctg
     8101 gcgcggatgg cagagcaggc gggggttaaa ttccgcttta atacgcccgt tgaccaactg
     8161 ctttgcgacg gcgagcaaat ctacggcgtg aagtgtggcg atgaagtgat taaggccgat
     8221 gcgtatgtga tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat tgttgatatt
     8281 ccggtgtatc cgctgaaagg ctactcgctg actattccaa ttgcgcagga agatggtgcg
     8341 ccagtatcca ccattcttga tgaaacctac aaaatcgcca ttacccgttt cgataaccgc
     8401 attcgtgttg gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt
     8461 cgtgaaacgc tggaaatggt ggttcgcgat ctctatccac gcggcggtca tgtcgagcag
     8521 gcgactttct ggacgggttt gcgcccgatg acgccagacg gtacgccggt tgtcgggcgt
     8581 acacgcttta aaaatctgtg gctgaatacc ggtcacggca cgctcggctg gacgatggct
     8641 tgcggttccg gtcagttgtt aagcgatctg ctctctggtc gcacgccagc gatcccatat
     8701 gaggatctaa gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc gggccattta
     8761 catggcgcac acagctaagg aaacgagatg acccgtccga tacaggccag cctcgatctg
     8821 caggcattaa aacagaatct gtccattgtc cgccaggccg cgccgcacgc gggtggtaaa
     8881 agcgaacgct tacgggcatg gtattgagcg tatctggagc gcgctcgggg ccaccgatgg
     8941 ctttgcatta cttaacctgg aagaggcaat aacgttacgt gagcgcggct ggaaggggcc
     9001 gatcctgatg ctggaaggat ttttccatgc tcaggatctg gagatttatg accagcaccg
     9061 cctgaccacc tgcgtacaca gcaactggca gctcaaagca ctgcaaaatg cgcggctaaa
     9121 agcaccgttg gatatttatc ttaaagtgaa cagtgggatg aatcggttgg gcttccagcc
     9181 cgatcgcgtg cttaccgtct ggcagcagtt gcgggcaatg gcgaatgttg gcgagatgac
     9241 cctgatgtcg cattttgccg aagcggaaca tcctgatgga atttccagcg cgatggcgcg
     9301 tattgagcag gcggcggaag ggctggagtg tcggcgttcg ttgtccaatt cggcggcgac
     9361 tctgtggcac ccggaagcgc attttgactg ggttcggcct ggcattattt tgtatggcgc
     9421 ttcgccgtcc ggtcagtggc gtgatatcgc caataccgga ttacgtccgg tgatgacgct
     9481 aagcagtgag attattggtg tccagacgct aaaagcgggt gagcgtgtgg gctacggcgg
     9541 tcgctatact gcgcgcgatg aacagcgaat cggcattgtc gccgcagggt acgccgacgg
     9601 ttatccgcgc cacgcgccta ccggtacccc tgttttagtg gacggcgtgc gcaccatgac
     9661 ggtggggacc gtctcgatgg atatgctggc ggtcgattta acgccttgcc cgcaggcggg
     9721 tattggtacg ccggttgagc tgtggggcaa ggagatcaaa attgatgatg tcgccgccgc
     9781 tgccggaacg gtgggctatg agttgatgtg cgcgctggcg ctacgcgtcc cggttgtgac
     9841 ggtgtaactt gttgtaagcc ggatcggagg caacgtcttc tgggtgcaaa aaaaatcatc
     9901 catccggctg gtcagcaact gtagttgtta atgtgacaga gccattgccc atgatagtgt
     9961 ccattaaaag gatggacact atttccccgg aacctgaact caccgcacag gcgttctaca
    10021 taaaacgctt acgcttcatt gttgactcgg agtcgacttc agataaatca cgctttaccc
    10081 ttgatggagc ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt caattcaaca
    10141 tcaaatgaag gttaaattat ggacgacaaa caattgcagg ctcaggctgc gttcagcaaa
    10201 gcatcgcaac cggcgataga tgcttcatta aatttaagat tcagcttcct cttcagccac
    10261 ccgtacgcca atcttcaaca cttcattatc ttctttctcg gccaccgtcc agatcatccc
    10321 ggcaaactct acctggtcac cgacaaccgg tgccgtgcct aacaactgct ggacaatttc
    10381 acccagcgtt tgctgcttat cacgatattc tcgcccgtct tccaggccat atatcagcgc
    10441 aacatcagca tatttggcgc tggcttcgag aatgaagtca ccaaagaagc gttgatccag
    10501 cgcgaccggc ggcgactggc tgaacagttt accgagcgcc ggaagatcgc gttcccgacc
    10561 aattacacac aacacatcgc cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg
    10621 gttatcacga aacagtgccg caatacgcgt ctcttttggc atatgcaaat cgcgcagtgc
    10681 cgcgcccacg caccatttat cggcactcaa ttgataaaca aactgctccc acggattttc
    10741 cggatgaata tccaggccaa cgcgtgacac cggacgtccc actggcggaa ccaccacttt
    10801 ggctttttta gccgcccacg agagtgatgt tccctgcaag agcagtgaaa ccagaaccac
    10861 aaagaaggcg acattaaaga acagacgtgc attctccagc cccgccatca tcgggaacac
    10921 ggccaggatg attggcaccg cgccgcgtaa tcctacccag ctgataaaca cgcgctcacg
    10981 cagattgaaa ccgcggaagg ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa
    11041 tatcatccat gccgacaaaa ttagcgccgg aatggcaata ggcagcagat cgcttgggtt
    11101 aaccaatagc cccagcacca ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa
    11161 attttgcagg atgccgtagc ggttgcgaat cggccgatta cccagcagaa aaccgcacag
    11221 ataaacagcc agaataccgc tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc
    11281 gccgcttaat gccagcaatg gatataatcc ggcaggcagg gcgatgcgat taatcatttg
    11341 cagcagtaaa taaccaccgc caagtccgat gacaatcccg aggccaaatt gctgcagaat
    11401 atcgaccacg aacatccaac tgacactgct ttcatgttgc tggatcatcg caatcagggt
    11461 aatcgtcaga aagaccgcca ttggatcatt actgccggat tcaatttcca gcgtcgagcc
    11521 aacacgttcg ttaagcccct taccacccag cagagaaaag accgctgcag catcggtaga
    11581 gccgacgata gcgccgatta ataagccttc aatcaaatca agattaaaca gccacgccgc
    11641 catcatgccg gttaaaccag aggtgataag cacgcccagc gtcgccagcg acagtgccgg
    11701 tcctaacgcc acacgaaagg aactggcctg agtgcgcatc ccgccgtcga gcaaaataat
    11761 cgccagtgcc aggttactta ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc
    11821 gacgccatcg actcctgcca gcatgccgat cgccagaaaa ataaccagaa taggaatgcc
    11881 aagacgggaa gaaaatgaac taagtaatat actgctggtg acaaggatgg atcctaagat
    11941 gaaaaggcta attattgttg tggcatccaa cggtcggtta ctcctgatta cgctgtctct
    12001 tatataaacc ctaccatatt agcggcagag acagcgtttt acttagccct gaagcgtgat
    12061 ttttttacat tttaagaaca ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt
    12121 tattcagaat ggcatgtgcg cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat
    12181 cgaggccggt aatgagtgga atcgacaggc gggaacgcaa aaacgcggac actgactcca
    12241 ggttgtaacc tgcgtcataa tcattgggcg tgctgccgct aaagctaccg agaataatcg
    12301 ccttctgacg tggcaaaatt cccgcatgat agagctgcaa cagcatacgt tcgacgcgga
    12361 aagggtgctc gttaatatct tccagtacca ggataccgtt ctcaattttt ggcatccacg
    12421 gtgtaccaat cagtgaaatc agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct
    12481 gacatgtcgg gccttcccct tgccattcaa tggtgaaggt tttattgcgt aacgccagcc
    12541 agaaatggtg ctcggtaaag gcgttcagtt catccgcacc aaagtttgct accagcatgg
    12601 ggccgctaaa ggtgatgaca ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat
    12661 cgctatgtcc gcaaatgagt aacgggtcat gttgttggcg agccaccagt gcctgccagt
    12721 caatatctgc cagtaaacga ctggcaccgt aaccgccgcg tacagccagc acgatggtgt
    12781 tgggtgtagt cagtctggca agggaattga gatcttccag acgctccgtt tccgtacccg
    12841 caaaacgctc acaacgacgg gcaataacct cgacgttatt gacttgatgc cccgcgtcgg
    12901 taaggcgctg gataccacgc agcgcggcgt gctgtttaat gctgtaaccc gatggggcaa
    12961 ttaagtgaaa cagagacatg gcaattcctt gctgacaaca gaaacgaaat gtatatcatg
    13021 ccgcttaggt gtgccgttgt cacctcaacg gcgattccag gctataagga tagaagaagt
    13081 gaaattgaga tggtttgcct ttttgattgt gttattagcg ggttgttcat caaagcatga
    13141 ctatacgaac ccgccgtgga acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc
    13201 aataagccag aaagccggtg cagcctgggg cgtcgatcca caattgatca cggcgattat
    13261 cgctatcgaa tcgggtggta atccgaacgc ggtgagtaaa tcgaatgcca ttggtttgat
    13321 gcagttaaaa gcttcaacct ccggacgtga tgtttatcgc cgtatgggct ggagtggtga
    13381 gccgacgacc agcgagctga aaaatccaga gcgtaatatt tcaatggggg cggcttacct
    13441 gaatattctg gaaaccggcc cgctggcagg cattgaagat ccgaaggtac tgcaatatgc
    13501 gctggtggtg tcatacgcta acggggcagg tgcgctgcta cggactttct cgtcagatcg
    13561 gaaaaaggcg atcagcaaaa tcaacgattt ggatgctgac gagttcctcg atcacgtagc
    13621 gcgcaatcat cctgcgccgc aggctccgcg ctatatctac aaacttgagc aggcactgga
    13681 cgcgatgtaa atcagtcgcg tactttgtcc gctttctccc gggcttcccg ctcgagagag
    13741 aaaataatcc gttgtaattg ccgctccacc gtcgggctga cgttaagaaa acggaagctc
    13801 agacggggag tggtgatggt ttcattcttg ccatcaatca ctttgcgctc gctgatggag
    13861 attaactggg cgtcaaagtg aaaaacaccc cattgcccca tgttgacttc aatctgagcg
    13921 aagcgcatgc cttcttgtaa ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg
    13981 cctaacgaca aatcatacag gcggaaacgt aacgtactgt tatccgccag tttggtctgg
    14041 caaaaataag gcggatggag tggggcggag atgcggaaat atcggcgtcg ttgtacaaac
    14101 cataaggtgg gaggcggtac ggtaataaat gccggaagct gcaagtattc actctgctgt
    14161 agttgttcaa cagtaaactc gactttcgca ccctgagttt cggcggtaat ggtaatatgc
    14221 tgcgccttta gcacggcgat gttgtcttcg gcttgactgc cgaaatccag caccagttta
    14281 tccggggtta ttgccagtat tttgctgatc agctgcccgc cattccaact gagacgcaaa
    14341 ggaattgcgg ctttgtgcaa atcgcgtaac acgcccagga cggctaacgg attttgtttc
    14401 aggaactgct catggtaatg actcacgcgg aaaaactcct gactgacaaa ctgtctttgg
    14461 tttatcggtc acagttaaca aaacttaata caaatgcgtg aatatttttt acatgttgtt
    14521 cttaaatcag ccgacatacg cccagcatcg atcccgccct tgcctatact tagagcgttg
    14581 acgtaagcat ttcttgcgtc cgattcatcg aaacgagggc ttgaacatgg gaattattgc
    14641 ctggattatt tttggcctga tagccggcat tatcgccaag ctaatcatgc cggggcgtga
    14701 tggtggtgga tttttccaga cctgtattct cgggatagtc ggtgcggtgg tcggcggctg
    14761 gctggcgacc atgtttggca ttggcggctc catcagcggt tttaatttgc acagcttcct
    14821 ggtggcggtg gtgggagcta ttctcgttct gggcttattc cgcctgctgc gaagagaata
    14881 agattttcat aaggcggata gcgatacaga tgccgctatc cgcttttcac atcagaacgt
    14941 atattcgaca cctgctttac gggtgaaaaa aatcaatata gcacttcgat atccgataaa
    15001 gtcggttccc ggtaacaaca acagaatcct tttttaatta ttgtttcgtt gtttttggtg
    15061 tgattcagaa aataaatatt ttagacagta gccagtcagg cgttaaaaat ggggcggata
    15121 gtgaggcggt tttggctgcg cttattgaac atttagaatt gatcaatcct tctggcagat
    15181 taagccaaaa tacccgttcg gcaatgcttc agttacgcga agaggaatgg tctgaattct
    15241 ttttttggtt gctaaattct ctggagtgtc tggattatgt gattattagt ttaactccag
    15301 aaagcaaaaa aacgttgatg agtgaacacc gtaacaatat tcaggtagca atcgatgctt
    15361 tatataggca acgtcggtgt aaatcaccgg gggatgatag tgaaacactt acccgtagaa
    15421 atgacgctat cttcggcaac cacgtatggc aaacatttgc gcaatacttc ccgccaggac
    15481 ttgaaaaacc ggctgtgtag aaaacaccat agctatggtg ttatctgccg acaacgccat
    15541 ccggagaagc cgaatgacgt tgtcggagta agcgctggtt aaggtgtggg ttgtgcctct
    15601 ttggttgagg gttgcgtcgt tgctgactta acggtcggac gcgtcgccgg aacattgtca
    15661 cacggttgct ctttcgggca gatcaaatcc agcattttca gcgtcacgcc attggtccag
    15721 ccaaagccat cctgtaatgg atattcgcca cctctttttg cccgtagttt tgtaatcctt
    15781 ctgtcgcgac ccactgtaac ggtgcccagc catttggcgc atcccattgt tgcccacttt
    15841 tcaccgacgt ggtgttcagg ccgccgggtt gcagcagatg tgttttcgtc gccgtcgcca
    15901 ttttgttggc gcgatctttc gctgccgcat tgacgtacag cgggaacagg gcggccgcgg
    15961 ttaactgatt gcgcacttta tgacttttca ggtcgtaatc ggcataccag ccttgttgat
    16021 cgttccacag gtatttttcg attccttttt gacgggcatt cgccagcgtt tcgtactggt
    16081 ttgccatcgc gttatctcct gcagctttgc tggcgcgggc gaggattttt tccattttaa
    16141 acatcaggct gttcagatcg accggtacga tgctggtggt gcgtaaggta tttaactgct
    16201 gcgggttgtc catccagcgc gagctgaaat cccagccaga cgcagcggca gagcgcaggt
    16261 cgcggtaaat ttcagtggca ggtcgattcg gattgctttt ggcggtggca atatcttcca
    16321 cccatgactc tggtcgtggc gtatcgcgat cgtcccagta gcggttgaga agggtaccat
    16381 cctgaagttt gacaacgcgt ttttcctgtt gtccggcttg caggttttca acaccgtcca
    16441 tccagtaagc atattctttt tgcatttgcg gcaggtattg cttcaacgcg gcatcgcctt
    16501 catgctgcgc cagtaactct accatcaggg caaagaaggg cggttgcgag cggcttaaat
    16561 agtaactgcg gttgccgttg ggaatatgac cgtaagtgtc tatttcatga gcaaaattgg
    16621 ccaccatatc cgcgacttta tcccagtgac cgctttcggc aagtcctaac atggtgaagt
    16681 aactgtccca gtaatatacc tcgcgaaaac gtccgcccgg cacgacataa ggttccggca
    16741 gcggtaacag agaatcccat ttttcggtgt tttcggtaga acgcgttaat accggccaaa
    16801 gtccgtcaat atgttcgcgc agtgactgcc cctctggcgg aacatatttc tcgccttctt
    16861 tcggcagggt gaaattgacg ttaacgaaat ggcgcagatc aaatccgctc tggttttgct
    16921 gcatccgata atcagcggat cgctgttcgg cacggcatcg gcaaaggttt tttggtccgg
    16981 aaaaagtttg gcgttttgca catcattaaa cagcggccct aataaaatat caggcggctg
    17041 tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca gcgaaacaca aaaagataca
    17101 ggct
//
LOCUS       DADLTD010000081        17030 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLTD010000081 DADLTD010000000
VERSION     DADLTD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.081"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..154
                     /locus_tag="JN701_002102"
     CDS             <1..154
                     /locus_tag="JN701_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HAY7364554.1"
                     /translation="NYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTL
                     VKSFDW"
     gene            429..3278
                     /gene="rcsC"
                     /locus_tag="JN701_002103"
     CDS             429..3278
                     /gene="rcsC"
                     /locus_tag="JN701_002103"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY7364555.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="JN701_002104"
     CDS             complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="JN701_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY7364556.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="JN701_002105"
     CDS             complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="JN701_002105"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY7364557.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLINKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7556..8686
                     /gene="ompC"
                     /locus_tag="JN701_002106"
     CDS             7556..8686
                     /gene="ompC"
                     /locus_tag="JN701_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7364558.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8798..9853
                     /gene="apbE"
                     /locus_tag="JN701_002107"
     CDS             8798..9853
                     /gene="apbE"
                     /locus_tag="JN701_002107"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY7364559.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9927..10979
                     /gene="ada"
                     /locus_tag="JN701_002108"
     CDS             9927..10979
                     /gene="ada"
                     /locus_tag="JN701_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY7364560.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10979..11629
                     /gene="alkB"
                     /locus_tag="JN701_002109"
     CDS             10979..11629
                     /gene="alkB"
                     /locus_tag="JN701_002109"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY7364561.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11705..13348
                     /gene="yojI"
                     /locus_tag="JN701_002110"
     CDS             11705..13348
                     /gene="yojI"
                     /locus_tag="JN701_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY7364562.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13566..15212
                     /gene="mqo"
                     /locus_tag="JN701_002111"
     CDS             13566..15212
                     /gene="mqo"
                     /locus_tag="JN701_002111"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAY7364563.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15362..15850)
                     /gene="eco"
                     /locus_tag="JN701_002112"
     CDS             complement(15362..15850)
                     /gene="eco"
                     /locus_tag="JN701_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY7364564.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15985..16149)
                     /locus_tag="JN701_002113"
     CDS             complement(15985..16149)
                     /locus_tag="JN701_002113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364565.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHVYIAINSPEI
                     FLLVCSLIRI"
     gene            16258..16752
                     /gene="napF"
                     /locus_tag="JN701_002114"
     CDS             16258..16752
                     /gene="napF"
                     /locus_tag="JN701_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY7364566.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16742..17005
                     /gene="napD"
                     /locus_tag="JN701_002115"
     CDS             16742..17005
                     /gene="napD"
                     /locus_tag="JN701_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY7364567.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
BASE COUNT         4147 a         4117 c         4435 g         4331 t
ORIGIN      
        1 caactactat ggtgggcgca gcgaaacgga attaaccacc gcccaactga cgctgatcac
       61 cgatgaagga tcggtcaatg agaaacagga aacatttatt gtaccgatgc gtaatgctgg
      121 cgagctgacg ctggtgaaaa gttttgactg gtgatccatc ccttcacaca acctggctct
      181 ctcttttttc agataagact gctgggcgat ctgccgacgc caggatataa ccattaacaa
      241 gcgtcggcag attggtcatt tcttattttt cgtactgaga actgtccatc tactccggct
      301 ttcctggtta aggcaatggt ttctaagcga tacgcattat tgcaataatg acagaacatt
      361 gcggtaacac gcttttaccg ctaccttaac tacactccat cggtcacctg aggcggagct
      421 tcgccccttt gaaatacctt gcttcttttc gtacaaccct gaaagcctcg cgctacatgt
      481 tcagagcatt ggcgttagtg ctctggctgt tgattgcttt ttcatccgtt ttttacatcg
      541 ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga atttaatctg agttccgatc
      601 aggctcagcg ctttattcaa cgcacctctg atgtgatgaa agagctgaag tacatcgccg
      661 aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg
      721 atgtgcctgc gtttgaaccg ctgtttgccg actccgattg ttccgcaatg agtaacacct
      781 ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg
      841 cggcttacga tctaaaccgg gtatttttaa tcggcagcga taacctctgc atggccaatt
      901 tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt gaaagctttg catgaacgca
      961 tcaataaata tcgaaatgca ccacaagatg atagcggcag taacctctac tggatcagcg
     1021 aaggtccgcg ccctggcgtc gggtattttt acgcgttgac gccagtttat ctggcgaacc
     1081 ggttgcaggc gcttttgggg gtcgagcaga ccatccggat ggagaacttt ttcttaccgg
     1141 gtacgttgcc gatgggggtt accattcttg atgaaaatgg tcataccctg atttcgctta
     1201 ccggaccaga aagtaaaatt aagggcgagc ctcgctggat gcaggaacgc tcctggtttg
     1261 gctatacgga agggttccgg gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa
     1321 gcatcgtgta ttcggtgccg gttgataagg tgctggaacg cattcgcatg ttgatcctta
     1381 acgcaatttt gctgaatgtg cttgccggag ctgcattgtt tactctcgca cggatgtacg
     1441 agcgacgtat tttcattccg gcggaaagcg acgccctgcg actggaagaa catgagcagt
     1501 tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg cattttgcgt accgctgatg
     1561 gcgtcaatat tttaagtaac gaactggcgc atacctatct caatatgctt acgcatgagg
     1621 accgccaacg actgacgcaa attatctgtg ggcagcaggt caattttgtt gatgtcctga
     1681 ccagcaacaa taccaatctg caaatcagct tcgtccattc gcgctatcgt aatgaaaacg
     1741 tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg
     1801 agatggcaca agcagcggaa caggcgagcc agtcaaaatc gatgttcctt gccaccgtca
     1861 gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa cctggatctg ttgcaaacca
     1921 aagagttacc gaaaggcgtc gatcggctgg tgacggcaat gaacaactct tccagcctgt
     1981 tgttgaaaat tatcagcgat attctcgatt tctcgaagat tgaatcggaa cagttgaaga
     2041 tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca catcaccgcc aactatttac
     2101 cgctggtggt acgcaagcag ttaggcttgt actgctttat tgaaccggat gtgccagtgg
     2161 ccttaaatgg cgacccgatg cgtttacagc aggtcatctc caacctgttg agtaacgcca
     2221 taaaattcac cgataccggc tgtatagttt tgcatgtccg cgctgatggc gattatctct
     2281 ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg
     2341 atcccttctt ccaggtcgga acgggcgtac agcgaaactt ccaggggacc ggtctgggtc
     2401 tggcgatttg tgaaaaactg atcagcatga tggacggcga tatctcggta gattcagaac
     2461 cgggaatggg cagccagttt accgtgcgta ttccgttgta cggcgctcag tacccgcaga
     2521 aaaaaggcgt ggaagggttg agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc
     2581 tctgtcagtt cctggaaact agtttgcagc gcagcggcat cgtcgttaca acatacgaag
     2641 ggcaggaacc gactcccgaa gatgtgttaa ttactgacga ggtagtgagt aaaaaatggc
     2701 agggtagagc ggtagtgacc ttctgtcgtc gccatattgg tattccgctg gagaaagcgc
     2761 caggggagtg ggtacacagt gtggctgccc cgcatgagct accggcattg ttggcgcgta
     2821 tttatttgat cgagatggag agcgacgatc ctgctaacgc tctgccgtcg acggacaaag
     2881 cggtcagcga taatgacgat atgatgattc tggtcgtgga tgatcatccg attaaccgac
     2941 gtctgctggc agatcagttg ggatcgttgg gctatcaatg taaaaccgcg aatgatggtg
     3001 tcgatgcgct taatgtactt agcaagaatc atattgatat cgtgcttagc gacgtcaaca
     3061 tgccaaatat ggatggttac cgcttgacgc aacgcattcg tcagttggga ctgacgttgc
     3121 cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg
     3181 gtatggacag ctgcctgtcg aagccggtaa cgctggatgt gataaaacag acgctgacgg
     3241 tatatgccga gagggtcagg aaatcgcggg aatcgtagga cggataaggt gtttatgtcg
     3301 catctggcat tcagtgcaaa tgccagatgc gacgctgacg cgtcttatct ggcctacttt
     3361 gatgtaatct gttgaaataa tgggaatcgt aggacgcata aggcgtttac gccgcatccg
     3421 gcaatcggtg caaatgccag ataagacact aacgcgtctt atctggccta caggtgatta
     3481 gtctttatct gccggactta acgtcactga agagagataa ttcagcaggg cgatatcgtt
     3541 ctcgacaccc agcttcatca tcgcagattt cttctggcta ctgatggttt taatactgcg
     3601 gttcagcttt ttagcgatct cggtcaccaa gaagccttcc gcaaacaggc gcagaacttc
     3661 actctctttt ggcgagagac gcttgtcacc gtaaccacca gcactgattt tttccaacag
     3721 gcgagaaacg ctttccgggg taaatttctt ccctttctgc agcgcggcga gagctttcgg
     3781 cagatcggtc ggtgcacctt gtttcagcac gatcccttcg atatccagat ccaataccgc
     3841 actaagaatc gccgggttgt tgttcatagt cagaacaatg atcgacaggc ttgggaaatg
     3901 gcgcttgatg tacttgatta aggtaatgcc atcgccgtac ttatcgccag gcatggagag
     3961 atcggtaatc aacacatgcg catccagttt cggcaggttg ttgatcagtg ctgtagagtc
     4021 ttcaaattcg ccgacaacat tcacccactc aatttgctca agtgatttgc gaataccgaa
     4081 caagactatc ggatggtcat cggcaataat tacgttcata ttgttcatgt aataggctac
     4141 cttgctacag caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg
     4201 gaacatcctt ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca
     4261 gatttagcat ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt
     4321 cactggttgc tgcttcagta tacagcctct taacatcatc cggtactgtg tctacaaaga
     4381 gcgcataata gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg
     4441 tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt
     4501 tgctcatatt gaagttgacg cacaattgac caggcccaat ttcccgtacg ccagactcat
     4561 catcgcttaa aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat
     4621 cataatcttg actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta
     4681 actggcgagt gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat
     4741 catccagtaa acgctcttct tcctcttcaa cttccgggtc agcggcgagc attttgatat
     4801 gcacagagta gcgtgtacca agcccatccc gcgttttgat gtttaaatga ccgcccagtt
     4861 tacgcgccag ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt
     4921 tttggggctg gttgataaac gggaagtgca aattatccat ttcatgaata cttacgcctt
     4981 ctcccgtgtc cagaatgcgg aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct
     5041 caagggtgat ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa
     5101 tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa
     5161 tcagcagttg cagaccttta cgcttgatgg caggcaacac tgaaggcaca acttcatcaa
     5221 ttaaatcctg cacggagaac agcaccgtct cacttttcca gctatcgtcc gcaagcatgt
     5281 tcgctaactg tatttcatcg accagccgca ccagcacatc tgcctgattc gccagttgtt
     5341 tgctttccgg ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg ggttctttca
     5401 gcgcatcgcc aatgtttttc ataaaggtca tccgcccctg ctggtttttc tcatacagac
     5461 gctgcgcctg cttgagtttc ttgtttatca gcacttcgcg atcctgatcg cgaataatga
     5521 aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg gatctcatac agctcgttat
     5581 tgatcgtcgc ctgaataatc ccctgatgct gttccgccat ggtggtgatg ttttgcagat
     5641 tcaaatgcgg cagcaaatga tcggcaattt tgttacttat gacagtgcgg ttcgattcct
     5701 gatcgtgaac cagcaggccg agcggcagca gtgagactat ctcttcatta attgcccgta
     5761 aaatgcgcaa ttcgttattg accgccgtgc tgggtagact ttctgtactg cggctggaga
     5821 aatggcggaa tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca
     5881 gtggcagcag aatgttttgc aacgtatcca gcaataaggt gccataagga acctgccaga
     5941 ccaggcgcat atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt
     6001 ggatactgac gctatccgtc ccttctttct cattatcatt gtctcccgtt gccgtcgcgt
     6061 ctggctcaag gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggta
     6121 gatcaaaagc tacgaccgtt gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa
     6181 agtaatgacc gttctgccag gccaggcggc gcatgttaga aaaactttcg cgttcatcga
     6241 gggcgttggc ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg ctgatggtcg
     6301 attctttaaa tccggaggtg agatctttga ggggcagggt tgagatcagc accagactgt
     6361 tatcctgacc attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtgt
     6421 ccagataggt ggacatccgc tgagtcatct caagcgttga gttgtcgtga gagccaaaga
     6481 ttaacgcttc cgttttgcgg cgcggtttct ccagatagta gacatcctgt ttcaggcgcg
     6541 tctcttgtaa accttcgccg gaggaggggg aggttgtcgc ggcaatgttg tcgtagatct
     6601 gccaggtcac gtaacgccag gtattgacgc gcttttggat agcgtgggta atgtcgacaa
     6661 tctggtaact tttatctttc agccatgcgt taacggcgct ttgtaccatt acacccatcg
     6721 tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg
     6781 aaaagcgggt cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac
     6841 ttcgcgaagt gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat
     6901 tgatgattct ctattctccc gtgatattta tcatcctgca ttgggggaaa tagatgtaca
     6961 agcgccattt tgctgtacaa ataacctaca aaaagcccaa cgaaattaat tgcaaataaa
     7021 cgatgattgc tggaaattat gctgatgtta attatttgtg aaatagttaa caagcgttat
     7081 agtttttctg tggtagcaca gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccgg
     7141 atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata
     7201 aataaagtta atgatgatag cgggagttat tctagttgcg agtgaaggtt ttgttttgac
     7261 attcagtgct gtcaaatact taagaataag ttattgattt taaccttgaa ttattattgc
     7321 ttgatgttag gtgcttattt cgccattcca caataatctt aaaaagttcc cttgcattta
     7381 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt
     7441 gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat
     7501 cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta ataacatgaa
     7561 agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc
     7621 tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcttgca
     7681 ctatttctct gacaacaagt cagaagacgg cgaccagacc tatgtacgtc ttggtttcaa
     7741 aggcgaaact caggttactg accagctgac cggttacggc cagtgggaat atcagatcca
     7801 gggcaatacc tctgaagaca acaaagaaaa ctcctggacc cgtgtggcat tcgcaggtct
     7861 gaaattccag gatgtaggtt ctttcgacta cggtcgtaac tacggcgttg tttacgacgt
     7921 aacttcctgg accgacgtac tgccagaatt cggtggcgac acctacggtt ctgacaactt
     7981 catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac accgacttct tcggtctggt
     8041 tgacggtctg aactttgctg ttcagtacca gggcaaaaac ggtagcgtaa gcggcgaagg
     8101 catgaccaac aacggtcgtg gcgctctgcg tcagaacggc gacggtgtcg gcggttctat
     8161 cacttatgat tacgaaggct tcggtatcgg tgctgcagtt tccagctcca aacgtactga
     8221 tgatcaaaat ggtagctaca tcagcaatgg tgtagttcgt aactacatcg gtactggcga
     8281 ccgtgctgaa acctacactg gtggtctgaa atacgacgct aacaacatct acctggctgc
     8341 tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc
     8401 acagaacttc gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc cgtctgtagc
     8461 atacctgcag tctaaaggta aaaacctggg tgtcatcaat agtcgtaact acgacgacga
     8521 agatatcctg aaatatgttg atgttggcgc gacctactac ttcaacaaaa acatgtccac
     8581 ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg acgctggcat
     8641 caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc gattgatatc
     8701 gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaactc agttttttgg
     8761 tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct ttacccgcgt
     8821 ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac aacccgccaa
     8881 aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct tctggcgtgc
     8941 cagcatcccg ggcattgatg ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca
     9001 gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt
     9061 taacgactcg caaagtttgt cgccgtggcc agtaagtgaa gcgatggccg aaatcgtcac
     9121 tacctcgctg cgcattggcg cgaagaccga tggtgcgatg gatattaccg tcgggccgct
     9181 ggtaaatctg tggggctttg gcccggaaca acagccggtt caaattccga gccaggaaca
     9241 gatcgacgag atgaaagcca aaactggttt acagcacctt acggtcatta atcagtcgca
     9301 tcagcaatat ctgcaaaaag acctgccgga tttatatgtc gatctctcta ccgtcggcga
     9361 aggttatgcg gcggatcact tggcacgctt gatggagcag gaagggattt cccgctatct
     9421 ggtgtcggtg ggcggcgcgc tgaacagccg tggtatcaac ggtgaaggcc tgccgtggcg
     9481 ggtagcgatt caaaaaccaa ccgataaaga aaacgcagtt caggctgtgg tggatatcaa
     9541 cggtcatggg attagcacct ctggcagcta ccgtaactat tacgaactgg acggcaaacg
     9601 tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt
     9661 gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct tgatggtact
     9721 cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt atatgatcac
     9781 caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct tccttgtcag
     9841 cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg gcagactaaa
     9901 ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact gacgatcaac
     9961 gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc
    10021 gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgccttg cgggaaaatg
    10081 tctccttcta cgcaaatgcc agcgaggcac tcgccgccgg atttcgcccc tgcaaacgct
    10141 gccaacctga taaagccaat gcccagcaac atcggttgga taaaatcacc cacgcgtgtc
    10201 gactgctgga acaggaaacg cctgtaacgt tggaagcctt agccgaccag gtggcgatga
    10261 gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct aaagcctggc
    10321 aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga
    10381 cgtctattct taacgccgga ttccccgaca gcagcagtta ctaccgcaaa gccgatgaaa
    10441 cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg gtgcgttacg
    10501 cgctggctga ttgtgcgctg ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg
    10561 cgatattgct gggcgatgat gacgcgacat taatcagcga gttgcagcag atgtttcccg
    10621 ctgccgacaa cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa gtgatcgcca
    10681 gcctcaatca acgcgatacg ccgctgacgt taccgctgga tattcgcggc actgcttttc
    10741 agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgta ctggctaacg
    10801 ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg
    10861 ctatcgtaat accctgtcat cgagtggtcg gtggtgatgg cacactttcc ggttaccgct
    10921 ggggcgtgtc tcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat
    10981 gttggatctg tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat
    11041 tttacggcgt tttgctttta acgctgcgga gcaactgatc cgcgatatta atgacgttgc
    11101 cagccagtcg ccgtttcgcc agatggtcac ccccggggga tataccatgt cggtggcgat
    11161 gaccaactgt gggcatctgg gctggacgat ccatcggcaa ggttatctct attcgcccat
    11221 tgatccgcaa acaaataaac cgtggcccgc catgccacaa agctttcatg atttatgtca
    11281 acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg
    11341 ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc
    11401 gccaattgtt tctgtttctc tgggcttacc cgcgattttt caatttggtg gcctgaaacg
    11461 taatgatccg ctcaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga
    11521 atcgcggctg ttttatcacg gtattcaacc gttgaaagcg gggttttatc cactcaccac
    11581 cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt
    11641 attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt
    11701 ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat
    11761 ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg
    11821 ccttatcgaa atggcggata ccagtctgct ggtattgccg gagtttctgg gattattgct
    11881 gctgttgatg gcagtcactc tcggatcgca actggcgctc accactttgg ggcatcactt
    11941 cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccacg tcgagcgcat
    12001 tgaacaactc ggtagcgcct cgttgttggc gggattaacc agcgatgtgc gcaatatcac
    12061 cattgctttt gtgcgtcttc cggaactggt gcaggggatc attctcacta tcggttccgc
    12121 ggcgtatctg tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcgat
    12181 caccatctgg ggtggttttg tactggtggc gcgggtgtac aaacatatgg cgaccctacg
    12241 tgaaaccgaa gacaagctgt acacggattt tcaaactgta cttgaagggc gcaaagagct
    12301 gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacatcc ccgatgcgca
    12361 agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg tgaactggtc
    12421 aaacatcatg atgctgggcg ctatcggcct ggtgttctgg atggcgaaca gtctcggttg
    12481 ggctgatacc aacgttgctg cgacctattc gttgacgctt ttattcctgc gtacgccgct
    12541 gctttcggcg gttggcgcat tgccgacgct gctgatggcg caggtggcgt ttaacaagct
    12601 gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa
    12661 ctggcaaacg ctggagctgc gtaatgtgac gtttagctat caggataacg cgttttccgt
    12721 tggtccgatt aatctcacca tcaaacgtgg cgagctactg tttctgattg gtggtaacgg
    12781 tagtggaaaa tcgacgctgg cgatgttgtt gacgggcttg tatcagccac aaagcggcga
    12841 aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt
    12901 ttcggcagtg tttaccgatg tctggctttt tgatcaactg ctggggccgg agggtaaacc
    12961 cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga
    13021 gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc
    13081 gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga
    13141 tcaggatcca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat
    13201 gggtaaaact attttcgcta tcagtcatga tgatcattac tttatccacg ccgaccgcct
    13261 gctggaaatg cgcaatgggc aacttagcga gctgacgggc gaagagcgcg atgccgcttc
    13321 gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg
    13381 tcaggtcaat atgctaaaaa tcccttcatt tctaataacc ctataattaa tcaacgaaat
    13441 tataatgttt ctaaaattag aatataattt ataaacatta tttaaatgtt gttacttaag
    13501 tgttaccttt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
    13561 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
    13621 cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg
    13681 gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga
    13741 tgaccatgct ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaataacg
    13801 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
    13861 gtatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
    13921 gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
    13981 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
    14041 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
    14101 aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc
    14161 gtacggaaat tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct
    14221 tgcagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta
    14281 atgacgataa cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca
    14341 ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat
    14401 cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt
    14461 cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg
    14521 gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag
    14581 tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg
    14641 atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata
    14701 atttcgatct ggtgaaatat ctggtgagtc aggtgatgtt gagtgaagag gatcgttttg
    14761 aagcgttgaa agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg
    14821 ggcagcgtgt gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta
    14881 ctgaagtggt cagtgaccag caagggacca ttgccgcgct cctgggagca tcgccagggg
    14941 cgtcaaccgc cgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt
    15001 ccagcccgca atggcaggct acgttgaaag cgatcgttcc gtcttatgga cgcaagctga
    15061 acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctggggctga
    15121 aatacgacaa gccgcaggca gcagatagta cgccaaaacc gcagttgaaa ccgcaacccg
    15181 ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc
    15241 ggatgtggcg tatactgata agacgcgcca gcgtcgcatc aggcaaccgg ctcgggcgtt
    15301 agatgttgga tgcggcgtaa ccgccttatc caaccaacgg ggtgccgtac ttcacaacag
    15361 tttagcgaac ttctgcgttg tcaattttct cttccgcctt ccagacgcgg tatttcacat
    15421 ctacattgtc tggcgtatac accacgatcg gcagcttgct gttgtaacgc agcattccag
    15481 catcgcccag atacgcggtg acaaatttct tctctttctt gccatccggg caggccatca
    15541 tcgtggaaac cggcgagcta actttatcaa agacataata atcatagccc cagccttcca
    15601 gcgttttgct ttccagcttc ccgccgagac gatgcaaatt gcaatcgact tccagcgtct
    15661 gaccgattaa cagttctact ttcagggtag attcatcttc ttgcggggtt aactgaatca
    15721 cctgacgctt catccctttt tcagcttgtg gataaggcgc gattttttcc agtggctgga
    15781 cgctttctgc cgcccaggcg gaagtggtag cgaaagcggc aaacaatact gcaggtagaa
    15841 tggtcttcat ttttttgctt tccttcttca tttataggtt ttcaccgatg atgtcgctga
    15901 aggttaacat attcataaga tttattaatt tgctttatga ataacttaaa gaatattcag
    15961 acaattttac ctgctctttt agtgttaaat tctgatgaga gaacaaacca gcagaaatat
    16021 ctctggtgaa ttaatcgcta tataaacatg tttataacca tttgaaatgt gagcaaaagc
    16081 ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta ccccgatcgg
    16141 ggtatgcatc tttgacacat cctttaatat cttagcggtt ataaaaatgg cttattaatt
    16201 atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa ggtcaatgtg
    16261 aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc cagtaacggt
    16321 atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac ccgttgcgac
    16381 gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg ctatccgagc
    16441 gttaatttca aaaataacga gtgcagcttc tgttacgctt gcgcgcaggc ttgccccgaa
    16501 tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat tggggacgcg
    16561 tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga gccaatggca
    16621 attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag ccaactttgc
    16681 aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc ggagtatctc
    16741 catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg aacgaatttc
    16801 agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca gcgacgcgcc
    16861 gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga tccaaaccat
    16921 tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc accagcagga
    16981 agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa
//
LOCUS       DADLTD010000082        17000 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.082, whole genome shotgun sequence.
ACCESSION   DADLTD010000082 DADLTD010000000
VERSION     DADLTD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.082"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(62..784)
                     /locus_tag="JN701_002116"
                     /pseudo
     CDS             complement(62..784)
                     /locus_tag="JN701_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(729..1108)
                     /locus_tag="JN701_002117"
                     /pseudo
     CDS             complement(729..1108)
                     /locus_tag="JN701_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(1244..1351)
                     /gene="rprA"
                     /locus_tag="JN701_002118"
     ncRNA           complement(1244..1351)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JN701_002118"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(1537..2649)
                     /gene="ydiK"
                     /locus_tag="JN701_002119"
     CDS             complement(1537..2649)
                     /gene="ydiK"
                     /locus_tag="JN701_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY7364568.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            3038..6094
                     /locus_tag="JN701_002120"
     CDS             3038..6094
                     /locus_tag="JN701_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7364569.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            6091..6501
                     /gene="menI"
                     /locus_tag="JN701_002121"
     CDS             6091..6501
                     /gene="menI"
                     /locus_tag="JN701_002121"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY7364570.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            6601..6789
                     /locus_tag="JN701_002122"
     CDS             6601..6789
                     /locus_tag="JN701_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364571.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            7336..7704
                     /gene="sufA"
                     /locus_tag="JN701_002123"
     CDS             7336..7704
                     /gene="sufA"
                     /locus_tag="JN701_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY7364572.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            7713..9200
                     /gene="sufB"
                     /locus_tag="JN701_002124"
     CDS             7713..9200
                     /gene="sufB"
                     /locus_tag="JN701_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY7364573.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            9210..9956
                     /gene="sufC"
                     /locus_tag="JN701_002125"
     CDS             9210..9956
                     /gene="sufC"
                     /locus_tag="JN701_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY7364574.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            9931..11202
                     /gene="sufD"
                     /locus_tag="JN701_002126"
     CDS             9931..11202
                     /gene="sufD"
                     /locus_tag="JN701_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY7364575.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            11199..12419
                     /gene="sufS"
                     /locus_tag="JN701_002127"
     CDS             11199..12419
                     /gene="sufS"
                     /locus_tag="JN701_002127"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY7364576.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            12432..12848
                     /gene="sufE"
                     /locus_tag="JN701_002128"
     CDS             12432..12848
                     /gene="sufE"
                     /locus_tag="JN701_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY7364577.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            12997..14001
                     /gene="ldtE"
                     /locus_tag="JN701_002129"
     CDS             12997..14001
                     /gene="ldtE"
                     /locus_tag="JN701_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY7364578.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(14065..14301)
                     /locus_tag="JN701_002130"
     CDS             complement(14065..14301)
                     /locus_tag="JN701_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY7364579.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(14612..16024)
                     /locus_tag="JN701_002131"
     CDS             complement(14612..16024)
                     /locus_tag="JN701_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HAY7364580.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            16581..16790
                     /gene="fumD"
                     /locus_tag="JN701_002132"
     CDS             16581..16790
                     /gene="fumD"
                     /locus_tag="JN701_002132"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY7364581.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELDKQAMETVINALVK"
BASE COUNT         4381 a         4054 c         4428 g         4137 t
ORIGIN      
        1 ataggatagg ccatcagccc aatcaattcg tattttgcgg taacatccat aattaactcc
       61 tttaacctct atgcttaatt gtgtttaacc gggtgctaaa tttacgctcg ccaaaccgca
      121 catcattctc aggaataata aaaacgcggt aatagcggat aaaaacaata attgcggtaa
      181 tcagggccag cagcgcgaaa gtaaaatcga gaacaatgat atattgaagg ccgatgttcg
      241 acagataacc ggtaatcagc agaataacaa agttagccag tccacccatc atcatataaa
      301 tactggtgac tttggctttg ctttttggga aaaactctga catgaccgaa acaccgagct
      361 gtaaaatgcc gccagctgct gagaaaccga taacaaaggc gccggcatta cacaccagtg
      421 gagaagggta cagatagata atggctgctg ttattgttgc cagtgcagag ttaaatacat
      481 tagcccagat gggccggacc atttttttca gtagtgcggc aaaaataaag acacagacca
      541 acgagcccat actgtaataa gagatggttt ttaatgcctc agcttctgac ataccagcaa
      601 aagccatcgc atatttgggc atccacacca caatcacata aaaggtcgag aatgcggcta
      661 caccgaacag taccgatgaa acaccttcca gccagactaa cggtttgctg ttcatttgcg
      721 gtaattcatt agctacgctg gcgtccacca actggctggg gaatttgctt ttcaacagca
      781 ttaccttcag cggctaccga ttgatagatc ttccagtcaa taaaattgcc atcagggtag
      841 actcgttgaa atgcggttaa gtcggggaaa aaacgcatgg tgttgttgcc gatgcggtta
      901 tttattttct ctatgatggc atgaagatgt ttttttcttt gctgacgcat agccaacagt
      961 tgctcgacta cacgatcagg aatggcacac ttgccatttt cccactgacg ccagctttca
     1021 ctgtcaccgg tctgggcaat ccaggtgcgc attcatcaat agtcatggca aaaatatgtc
     1081 ggagtgcctg gagttcataa gcgttcatcg tgtaatggct gcatgaaata aacaataaaa
     1141 tataaataac attatcagcc tgctgacggc ttgaagagag tcacagtatc ttgtgcaaca
     1201 ttattggaaa gagtgagggg cgaggtagcg aagtggaaaa atgttaaaaa aaagcccatc
     1261 gtgggagatg ggcaaagact acacacagca attcgttgtt tcactcaggg gatttccatg
     1321 cttataaatc aatatgttga tttataaccg tgagctaata gtaggcatac ggactttttg
     1381 cgtcgatcag attcgtctca ttagtttaaa aactgtaaag aatttgcgac aggtactacc
     1441 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcgggg
     1501 gttaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
     1561 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
     1621 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
     1681 cccgaaagca atcaaaccac caataacacc agagaggatc aggatcagcg gtaaatcggc
     1741 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
     1801 cactaacaat accgttcccc aggtggtatc gccagtccag tagagccaga taatcgccgg
     1861 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
     1921 caacgttgca taaggtacgc cggatacggc gaggccgata ccgccaagca ctgcctgtac
     1981 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagtac
     2041 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
     2101 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
     2161 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
     2221 gcggactttc gccatgatcg ccgtcccccc catatccagc aagttgtgcc agcctgcata
     2281 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
     2341 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
     2401 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     2461 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     2521 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     2581 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     2641 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     2701 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     2761 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     2821 cgatttactc gtcgtatttc aagttgcatg tgctgcggct acgttcgttc accccagtca
     2881 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     2941 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     3001 cgacctacat cacagaccgc aggaaagggg caatataatg attccacaga tttctcaggc
     3061 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     3121 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     3181 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     3241 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     3301 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     3361 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     3421 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcgccgg aactttcgac
     3481 cagcaacagg gcaacgctcg gtgggatgat caataccgat gcatccggtc agggatcgct
     3541 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     3601 tgttctcgat acgcaacctt tacccgtcga attggcggaa acgctgggta aatccaatac
     3661 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtcagcaac gccagttaat
     3721 tatcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     3781 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     3841 ggcctttatt accgaagcgc ggctggatat tacgcgcttg cctaaagtgc gccgtctggt
     3901 gaacgtcaaa tatgactctt ttgactctgc gctgcgtaac gcgccgttta tggttgaggc
     3961 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     4021 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gaccaagaga tgctcgggct
     4081 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gatgagcggg taaatgcact
     4141 ctgtgcgcgg ttggatgagc tgatcgccag tcatcaagca ggtgtcatcg gctggcaggt
     4201 gtgccgtgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     4261 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     4321 gccggaacac ctggcggatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     4381 aagctacggt atgttcggtc acgtcgacgc aggtgtcttg cacgtccgtc cagagctgga
     4441 tatgtgcgat cctcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     4501 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     4561 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     4621 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     4681 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     4741 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     4801 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     4861 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     4921 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttgcggacgt taattgcccg
     4981 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     5041 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc ctgtgcccca tcaaaattga
     5101 tgtgccggag tttcgctctc gttttctgca gctctatcac acccgttatt tacgcccgct
     5161 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcgccg ctgatggcac gcgcgccgaa
     5221 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     5281 catggttgat ttgccgctgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     5341 ctcggcaaac atgacgctgg aacagcttga atctctcaat gcagagcaga aagcgcgcac
     5401 agtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     5461 tgtccgtctg gtcgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     5521 caaagcccag catattaaag gttttcttaa tcgttttgcg aagacggcga aaaagacggc
     5581 ggatttcctc aaccgtatgg cgaagctggg tatgccaatg gtgggcgtcg atccggcgct
     5641 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggagcgtg gcgagtttaa
     5701 cgtcttactg gcgaatgaat ggctggcaag cgcacttgag tcacagcctg tggctacagt
     5761 cagcggtgaa tcatggtatt tctttggtca ctgtaccgaa gttaccgcct tgccgggtgc
     5821 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     5881 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     5941 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     6001 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     6061 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     6121 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     6181 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     6241 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     6301 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     6361 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     6421 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     6481 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     6541 cattatctgc acagtaaacg gtggtatact gtacacgtct tgtctaaaag cgaggatgct
     6601 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     6661 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     6721 ctgcttactc tctcttctgc tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     6781 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     6841 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     6901 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     6961 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     7021 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     7081 ctcttaaata gtcattttaa tacaactttt tctttttcat cacagcctta atgctcctta
     7141 gataataacc attatctaac aatgagatac ctaattctta gcgtgcctgt taacccacac
     7201 atcagggtct atgcttatta aaagcaggca aaaagcatgt tttacggtaa agcccctgcg
     7261 tttgctgggt tgaactgata atcattatca ccaacatgct gttatacgct gaaagcgatg
     7321 aagtgaggta aatcgatgga catgcattca ggaaccttta acccacaaga tttcgcctgg
     7381 caaggcttaa cgctgacacc cgcagcggcg atacacatcc gtgagctggt ggcaaagcag
     7441 ccgggtatgg tcggcgtgcg tttaggcgtg aagcaaacgg gctgcgcggg ctttggctat
     7501 gtgctcgaca gtgttagcga gccggacaaa gacgatctgc tgtttgaaca cgacggcgcg
     7561 aagctgtttg tcccgctgca agcgatgccg tttattgatg gcacggaagt cgatttcgtt
     7621 cgtgaaggac ttaatcagat attcaaattt cacaacccta aagcccagaa tgaatgtggc
     7681 tgtggcgaaa gctttggggt ataggcggta ctatgtctcg taatactgaa gcaactgacg
     7741 atgtcaaaac ctggaccggc ggcccgctga attataaaga aggattcttc acccagttag
     7801 ccaccgatga gctggcaaag gggataaacg aagaggtggt gcgcgcaatt tcggcgaagc
     7861 gtaatgagcc ggagtggatg ctggagtttc gtctaaacgc ctatcgcgca tggctggaga
     7921 tggaagaacc gcactggttg aaagcgcact acgacaagct gaattatcag gattacagct
     7981 actactcagc accatcgtgc ggtaattgtg acgacacttg cgcgtctgaa cctggcgcgg
     8041 tgcagcaaac tggcgcgaac gcctttttaa gtaaagaggt ggaggcggcg tttgagcagt
     8101 tgggcgttcc cgtgcgggaa ggcaaagagg tggcggtgga tgccattttc gactctgttt
     8161 cggttgccac tacttatcgc gaaaaactgg cggagcaggg aattattttc tgttcctttg
     8221 gtgaggcgat ccacgatcac ccggaactgg tgcgtaaata tctcggcacc gtggtgccgg
     8281 ggaatgacaa cttctttgcc gcgcttaatg cggcggtagc ctctgacggt acgtttattt
     8341 atgtgcctaa aggcgtgcgc tgcccgatgg aactttccac ctattttcgc attaacgcgg
     8401 aaaaaaccgg gcagtttgag cgcaccattc tggtggccga cgaagacagc tacgtcagct
     8461 acattgaagg ctgttccgct ccggtgcgtg acagctatca gttacacgcg gcagtggtgg
     8521 aagtcatcat ccataaaaac gccgaggtga aatattccac ggtacaaaac tggttccctg
     8581 gcgataacaa caccggcggt attctcaact tcgtcaccaa gcgtgctttg tgcgaaggcg
     8641 aaaacagcaa aatgtcatgg acgcaatcag aaaccgggtc cgcgattacg tggaaatatc
     8701 ccagctgcat tttgcgcggc gataactcca ttggtgagtt ttactcagtg gcgctgacca
     8761 gcggtcatca gcaagcggat accggcacca agatgatcca catcggtaaa aacaccaaat
     8821 cgaccattat ctcgaaaggg atctctgccg gacatagtca gaacagttat cgcggcttag
     8881 tgaaaatcat gccgacggca accaatgcgc gcaatttcac tcagtgcgac tcaatgctga
     8941 ttggcgctaa ttgtggggcg cataccttcc cgtatgtcga atgtcgcaat aacagcgcac
     9001 aactggagca cgaggcaacg acatcacgta ttggtgaaga tcaactgttt tactgcctgc
     9061 aacgcgggat cagcgaagaa gacgccatct cgatgattgt taacggtttc tgcaaagacg
     9121 tgttctcaga actgccgctg gaatttgccg ttgaagcaca aaaactcctc gcgatcagtc
     9181 ttgaacacag cgtcggataa ggaataaaca tgttaagtat taaagattta cacgtcagcg
     9241 tggaagataa agctatcctg cgtggattaa gcctcgccgt tcgtcccggc gaagttcacg
     9301 ccattatggg gccaaacggt tcgggcaaaa gtaccttatc ggcaacgctt gccgggcgcg
     9361 aagattatga agtgacgggc ggcacggttg agttcaaagg caaagatttg cttgcactgt
     9421 cgccggaaga tcgtgcaggc gaaggcatct ttatggcctt ccagtatccg gtggagattc
     9481 caggcgtcag taaccagttt ttcctgcaaa cggcacttaa tgcagtgcgc agctatcgcg
     9541 gccaggaaac gctcgaccgc tttgattttc aggatttgat ggaagagaaa atcgctctcc
     9601 tgaagatgcc ggaagattta ttaacccgtt cggtaaacgt tggtttttcc ggcggcgaga
     9661 aaaagcgcaa cgatattttg caaatggcgg tgctggaacc ggagttatgc attcttgatg
     9721 agtcggactc cgggctggat attgacgcat taaaagtggt cgccgatggc gtgaactccc
     9781 tgcgtgatgg caagcgctca ttcatcattg ttacgcacta ccaacgcatt ctcgactaca
     9841 tcaagcctga ttacgtccat gtgctatatc agggacgaat tgtgaaatcc ggcgatttca
     9901 cgttggtcaa acaactggag gagcagggtt atggctggct taccgaacag cagtaacgcg
     9961 ctgcaacagt ggcatcactt gtttgaagct gaagggacaa aacgctcccc gcaagcacag
    10021 cagcatttac aacaattgct gcgtaccgga ctgccgacac gtaaacatga aaactggaaa
    10081 tatacgtcgc tggaagggct gaccaatagc cagtttgtca gcattgcggg ggagatatcc
    10141 ccacagcagc gtgatgcctt agcgttaacg ttagacgccg tgcggctggt gtttgtcgat
    10201 ggacgttacg tgcccgcgct gagcgatgca attgaaggca gcggatatga agtgagcatt
    10261 aacgacgacc gtcaggggtt acccgacgct attcaggcgg aagtgtttct gcatttgacg
    10321 gaaagcctgg cacaaagcgt gacgcatatc gccgtgaagc gcggtcaacg gccggcaaag
    10381 ccattgctgt taatgtatat cacccagggc gtggcaggtg aagaggtgaa cactgcccat
    10441 taccggcatc atctggatct ggcggaaggt gccgaagcaa cggtgatcga acattttgtc
    10501 agccttaatg atgctcgtca ttttaccggg gcacggttca ctatcaatgt cgcagcgaac
    10561 gcccacttgc agcatatcaa gctggcgttt gaaaacccgc tcagtcacca ctttgctcat
    10621 aacgatttgt tgctggctga ggatgccacc gcatttagcc atagtttcct gctgggtggc
    10681 gcagtgttac gacacaacac cagtacgcaa ctcaatggcg aaaacagcac gctgcggatc
    10741 aatagcctgg cgatgccggt gaaaaacgag gtgtgtgata cccgtacctg gctggaacac
    10801 aataaaggtt tttgtaacag ccgacagttg cacaaaacta tcgtcagcga caaaggccgc
    10861 gcggtattta acggtttgat caacgtcgcg cagcacgcca tcaaaacgga tggtcagatg
    10921 accaacaata atctgctgat gggcaaactg gcggaagtgg atacgaaacc gcagctggaa
    10981 atctatgcag atgatgtgaa atgcagccac ggcgcgacgg tggggcgtat tgatgatgaa
    11041 cagatgttct atctgcgctc gcgcgggatc aatcagcagg atgcccagca gatgatcatt
    11101 tacgcctttg ctgccgaact gacggaagca ctgcgtgatg aggggcttaa acagcaggtg
    11161 ctggcccgaa tcggtcaacg gctgccagga ggtgcaagat gactttttcc gtcgacaaag
    11221 tgcgggccga ctttccggtg ctttcgcgtg aggtaaacgg tttgccgctg gcttatctcg
    11281 acagcgccgc cagtgcacag aaaccgagcc aggtgattga cgccgaggcc gagttttatc
    11341 gtcatggcta cgcggcggtg catcgcggta ttcatacctt aagcgcccag gcgaccgaga
    11401 aaatggagaa cgtgcgcaag cgggcatcgc tgtttattaa tgcccgttcg gcggaagagc
    11461 tggtgttcgt ccgtggcacg acggaaggga tcaatctggt cgccaatagc tggggcaaca
    11521 gcaatgtgcg ggcgggcgat aacatcatca tcagtcagat ggagcaccac gctaacattg
    11581 tcccctggca gatgctttgc gcacgcgttg gcgcagagct gcgtgtgatc cctctcaacc
    11641 ccgacggtac gctgcaactg gagacgctgc atacgctgtt tgatgagaaa actcgcctgc
    11701 tggcaattac tcatgtctcc aacgtgcttg gcacagaaaa tccactggcg ggaatgatca
    11761 cgcttgcgca ccagcatggc gcaaaagtgc tggtggatgg cgctcaggcg gtgatgcatc
    11821 atccggtgga tgttcaggcg ctggattgcg atttttacgt gttttccggg cataaactgt
    11881 atggccccac cgggattggc attctttatg tcaaagaagc cttgttgcag gagatgccgc
    11941 cgtgggaagg gggcggttct atgatcgcca ccgtcagcct gagtgaaggc actacctgga
    12001 ccaaagcacc atggcggttt gaagccggta cacccaatac cgggggcatc attggtcttg
    12061 gcgcggcgct ggaatatgtt tcggtgctgg ggcttaataa catagccgag tatgaactga
    12121 atctgatgca ttacgcgcta tcacagctgg aatctgtacc gaatctcact ctctatggcc
    12181 cacaaaacag gcttggcgtt attgctttta atctcggtaa acaccacgcc tatgatgttg
    12241 gcagttttct cgataattac ggcattgctg tgcgtaccgg acatcactgc gctatgccat
    12301 taatggccta ttacaacgtc cctgcgatgt gtcgggcgtc gctggccatg tataacactc
    12361 atgaagaagt ggatcgtctg gtgaccggcc tgcaacgtat tcaccgtctg ctgggataac
    12421 agggaggcac tatggcttta ttgccggata aagaaaaatt gctgcgtaat tttttacgct
    12481 gcgccaactg ggaagagaaa tatctctaca ttattgagct gggccagcgt ctgccagaat
    12541 tacgcgacga agaccgaagc ccacaaaata gcattcaggg ctgccagaat caggtgtgga
    12601 ttgtcatgcg ccagaatgcg cagggaatta ttgaattgca gggcgacagc gatgcggcga
    12661 ttgtgaaagg gcttattgcg gtcgtattta ttctctacga tcagatgacg ccgcaggata
    12721 ttgtcaattt cgatgtgcgt ccgtggtttg aaaaaatggc gctcacccaa catctcaccc
    12781 catctcgttc acaaggtctg gaagcgatga ttcgcgcaat tcgcgccaaa gccgctgcac
    12841 ttagctaaac taaagggaca gctttcatcc ggttggctca cattgccgga tggtcaatgt
    12901 ttacactgac ctgcacagga gtgttttggt ttcaggtgaa cataagcgcc atccgacgtt
    12961 tcagcgtgag tctccggcaa atacaggagg tttacaatga aacgcgcgtc tttgcttaca
    13021 ctcacgctta tcggcgcttt tagcgccatc caggctgcct gggcggttga ttatccgcta
    13081 ccaccaaccg gaagccgact ggttgggcaa aatcaaacgt atacggtgca agaaggggat
    13141 aaaaaccttc aggctattgc ccgacgtttt gatactgcgg caatgttgat ccttgaagcc
    13201 aataacacta tcgccccggt gccaaaacct ggtacgacga taactattcc ttcacaactg
    13261 ttattacctg atgcaccgcg tcaggggatt atcgttaacc ttgcagagct gcgcctttat
    13321 tattatccgc cgggagaaaa tatcgtgcag gtttatccga taggtattgg cttgcagggg
    13381 ctggaaacgc cggtgatgga aacgcgtgtg gggcagaaaa tccctaatcc aacctggacg
    13441 cctacggcag gcattcgtca acgttcgctg gagcgtggca ttaaattact gccagtcgtt
    13501 cctgccggac caaataaccc gctaggacgt tacgcactgc gcttcgcgca tggtaatggc
    13561 gaatacctca ttcatggtac cagtgcgccg gacagcgtcg gtttacgcgt cagttcgggc
    13621 tgtattcgca tgaatgcacc ggatattaaa gccttattct ccagcgtgcg gacgggaacg
    13681 ccggtgaaag tgatcaacga accggtgaaa tattccgtgg agcctaacgg gatgcgttat
    13741 gttgaagtac atcgaccact atcggcagaa gaacagcaga acgttcagac aatgccatac
    13801 acactgccag caggctttac gcaatttaaa gacaataagg ctgtagatca gaagttagtc
    13861 gataaagcgt tgtatcgtcg ggcagggtat ccggtttcgg tgagcagtgg agcaactccc
    13921 gcagccagca atgcgccttc agtagagtca gcgcagaatg gtgaaccaga gcaagggaat
    13981 atgttacgcg cgaggcagta gtggtaaata gcagacaaaa aaaatggcgc acaatgtgcg
    14041 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    14101 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    14161 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    14221 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    14281 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    14341 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    14401 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaattgagag
    14461 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    14521 atttgatttt ttattgcttc tggttatcga ttaaataaaa aaagcgccca tcagggcgct
    14581 tcgatataca aattaattca caaaagcaat attacaggac gtgaacagat gcggtgttag
    14641 tagtgccgct cggtaccagt gcaccagaaa ccataactac aacgtcacct ttgtgtgcca
    14701 gaccgctctg cagagccagt tctttaccca gacggtagaa atcatcagta gaagtgatct
    14761 ctttaaccag ctgcggcaca acgcctttgc tcagtaccaa ctgatgagcc gttttttcgt
    14821 tggtggtcag tgccaggatg gtggcatccg ggaagtattt acgtactgcg cgagcagatt
    14881 taccgccctg agtagcaacc acgatcagcg gagcatccag tttttcagca gtttcaacgg
    14941 caccacggca taccgcttcg gtaatgcgca gtttacggtt gtcattgttg aactcgagac
    15001 ggctgttcat cacgcggtcg gtacgttcgc agatggtcgc catgatagaa accgcttcca
    15061 gcgggtattt accttttgcg gattcaccag acagcatcac tgcatcagta ccgtcgagga
    15121 tggcgtttgc aacgtcaccg gcttctgcgc gagtcgggcg tgggtttttg atcatggaat
    15181 ccagcatctg ggtcgcagtg ataacgactt tacgtgcacg gatacatttt tcgatcatca
    15241 tcttctgggc gaagataact tcttctaccg ggatttctac acccaggtcg ccacgcgcaa
    15301 ccatgatgcc gtcagaggct tcgaggattt cgtcgaagtt gttgaggcct tcctggtttt
    15361 cgattttgga gatgatgtgg atgttttcgc cgccgtgcgc tttcaggtgc tcacggattt
    15421 cgataacgtc agaacgctta cgaataaagg aagcagcaac aaagtctacg ccttgttcgc
    15481 aaccaaagat caggtcctgt ttgtcttttt cagccagtgc tggcagagca atggaaacgc
    15541 caggcaggtt cacacctttg ttttcgccca ggtcaccgtt gttcagcact ttacagataa
    15601 ctttgttacc ttcaatggcg gtaacttcca taccgatcag accatcgtca accagtacgg
    15661 tgttgccaac tgacaggtca gtagtgaaac cttcatacgt taccgcaacc atttcgctgt
    15721 tgccgataac agatttatca gtggtgaaag taaaggtctg accagctttc agagaaacgt
    15781 cgttaccgcc ttccagtttc atggtgcgga tttccggacc tttggtatca agcaggatag
    15841 cggcggtttt accagttttg ctcatcacgt tgcgcagatt ctgaatgcgc tgaccgtgtt
    15901 ctgcatagtc accatgagag aagttcagac gcataacgtt catgccagcg tccagcattt
    15961 tagctaacat ctcttcagat tcggttttcg gtccgatggt gcaaacaatt ttggtctttt
    16021 tcatgacagt cttagtcttt aagttgagaa ggatgggaga aacttgcttt ctgggcgcac
    16081 tgccggaaag cgaatccggt attacgaaag tggtggtgcc tcgctctaag gataggtgac
    16141 atcgaaaaag cgtgcagagg aatgtgtgct tgtgtctcag cccaacgtga gtgcctgttt
    16201 ttgagtcgtt gagttctaca gtccaaagtg ctgaaaccat tcaagagtca attggcgcgc
    16261 attatacgct gaaattaatc caaaaggaaa acaaaaacag cgtttcaaaa aatatacaac
    16321 ttgttagaca ataaattgtt atcaaagatt taacgttcca gggaaaaggt tacgcaaaaa
    16381 cttcgctgtt tgcgcaacgt ctggtgccaa agttgtcagt gtgaggataa gtgaaagttc
    16441 atccggtgta taaatatatc atcgctcaat tgctacagtt aaaataattt atcgcgttat
    16501 ttgtctgatt ttgtttgatt tttgtttaat tggttagatt gatatatgct gatagttggc
    16561 atgataaata ggtataaaga atgggaaatc ggacaaaaga ggatgaactg taccgagaaa
    16621 tgtgcagagt tgtcggtaaa gttgtgctgg aaatgcgcga tctggggcag gaaccaaagc
    16681 atattgttat cgcaggcgta ttacgtacag cattagccaa taaacgcatt cagcgcagtg
    16741 agctggataa gcaggcgatg gaaaccgtca ttaacgcact ggtaaaatga ccattcagga
    16801 tgaggcggta agcttcatcc tgatattctt gcttcagttg cggtccagtt aatcattcta
    16861 aataacaact gctgaaatta tacgaattgc ggtatgtatt ttaatttata gcgataatat
    16921 tatttttatt ttctgcaatg aacgtcacaa ataattgtcg cttaagttaa atttatttca
    16981 aatgataata aatatcagtt
//
LOCUS       DADLTD010000083        16904 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLTD010000083 DADLTD010000000
VERSION     DADLTD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.083"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            2..649
                     /locus_tag="JN701_002133"
     CDS             2..649
                     /locus_tag="JN701_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HAY7364582.1"
                     /translation="MMDETVTAIERAEQEKNALAQADGNDADDWRTAFRAAGGVLSDE
                     LKQRHIERVARRELVQEYDNLAVVLNFERERLKGACDSTATAYRKAHHHLLSLYAEHE
                     LEHALNETCEALVRAMHLSILVQENPLANTTGHQGYVAPEKAVMQQVKSSLEQKIKQM
                     QISLTGEPVLRLTGLSAATLPHMDYEVAGTPAQRKVWQDKIDQQGAELKARGLLS"
     gene            646..1146
                     /locus_tag="JN701_002134"
     CDS             646..1146
                     /locus_tag="JN701_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transactivation protein"
                     /protein_id="HAY7364583.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            1220..1792
                     /locus_tag="JN701_002135"
     CDS             1220..1792
                     /locus_tag="JN701_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HAY7364584.1"
                     /translation="MESTALQQVFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAT
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            2190..3269
                     /locus_tag="JN701_002136"
     CDS             2190..3269
                     /locus_tag="JN701_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364585.1"
                     /translation="MNVGHLNFFKVNKCGLYKVNDDNTYGLELSETFDLIQDWVGTKS
                     LALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDTDSTGSLLGASEDGEIGS
                     SSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSVCDSELFQDYVHSSITNR
                     VKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLETTHTELGKLIPKITHIV
                     RRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKY
                     SAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKE
                     FISPILKELKALQSQPRIKKASGED"
     gene            3270..3806
                     /locus_tag="JN701_002137"
     CDS             3270..3806
                     /locus_tag="JN701_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364586.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            3757..4767
                     /locus_tag="JN701_002138"
     CDS             3757..4767
                     /locus_tag="JN701_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364587.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(4822..6087)
                     /locus_tag="JN701_002139"
     CDS             complement(4822..6087)
                     /locus_tag="JN701_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7364588.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(6251..6335)
                     /locus_tag="JN701_002140"
     tRNA            complement(6251..6335)
                     /locus_tag="JN701_002140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6299..6301),aa:Leu,seq:caa)
     gene            6531..7550
                     /gene="ahr"
                     /locus_tag="JN701_002141"
     CDS             6531..7550
                     /gene="ahr"
                     /locus_tag="JN701_002141"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY7364589.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGVVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            7680..9182
                     /locus_tag="JN701_002142"
     CDS             7680..9182
                     /locus_tag="JN701_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY7364590.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9343..10425)
                     /gene="lptG"
                     /locus_tag="JN701_002143"
     CDS             complement(9343..10425)
                     /gene="lptG"
                     /locus_tag="JN701_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7364591.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(10425..11525)
                     /gene="lptF"
                     /locus_tag="JN701_002144"
     CDS             complement(10425..11525)
                     /gene="lptF"
                     /locus_tag="JN701_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7364592.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAPGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11792..13303
                     /gene="pepA"
                     /locus_tag="JN701_002145"
     CDS             11792..13303
                     /gene="pepA"
                     /locus_tag="JN701_002145"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY7364593.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            13463..13906
                     /gene="holC"
                     /locus_tag="JN701_002146"
     CDS             13463..13906
                     /gene="holC"
                     /locus_tag="JN701_002146"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY7364594.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13906..16761
                     /gene="valS"
                     /locus_tag="JN701_002147"
     CDS             13906..16761
                     /gene="valS"
                     /locus_tag="JN701_002147"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY7364595.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16817..>16904)
                     /locus_tag="JN701_002148"
     CDS             complement(16817..>16904)
                     /locus_tag="JN701_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY7364596.1"
                     /translation="PLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         4476 a         4071 c         4385 g         3972 t
ORIGIN      
        1 cctgatggat gagaccgtca cggccattga acgggcagag caggaaaaaa atgcgctggc
       61 gcaggccgac ggaaatgacg ctgacgactg gcgcacggcc tttcgtgcag ccggtggtgt
      121 cctgagcgac gagctgaaac agcgccacat tgagcgcgtg gcacgccggg agctggtaca
      181 ggaatatgac aatctggccg tggtgctgaa ttttgaacgt gaacgcctga aaggggcgtg
      241 tgacagcacg gccaccgcct accggaaggc acatcatcac ctgctgagtc tgtatgcaga
      301 gcatgagctg gaacacgccc tgaatgaaac ctgtgaggcg cttgtccggg caatgcatct
      361 gagtatcctg gtacaggaaa atccgctcgc caacaccacc ggccatcagg gctacgtcgc
      421 accggaaaag gctgtcatgc agcaggtgaa atcatcgctg gaacagaaaa ttaaacagat
      481 gcaaatcagc ctcaccggcg agccggttct ccggctgacc ggactgtcag cggcaacact
      541 cccgcacatg gattatgagg tggcaggcac accggcacag cgcaaggtgt ggcaggacaa
      601 aatagaccag cagggagcag agcttaaggc cagaggactg ctgtcatgat ttactgcccg
      661 tcgtgtggac atgttgctca cacccgtcgc gcacatttca tggacgatgg caccaagata
      721 atgattgcac agtgccggaa tatttattgc tctgcgacat ttgaagcgag tgaaagcttt
      781 ttctctgact gtaaagattc aggaatggaa tacatttcag gcaaacagag ataccgcgat
      841 tcactgacgt cagcctccgg cagtatgaaa cgcccgaaaa gaatgcttgt taccggatat
      901 tgttgtcgga gatgtaaagg ccttgcactg tcaagaacat cgcggcgtct gtctcaggaa
      961 gtcaccgagc gtttttatgt gtgcacggat ccgggctgtg gtctggtgtt taaaacgctt
     1021 cagaccatca accgttttat tgtccgcccg gtcacgccgg acgaactggc agaaagcctg
     1081 catgaaaaac aggaactgcc gccagtacgc ttaaaaacac aatcatattc gctgcgtctg
     1141 gaatgagggc tgccggttaa caccggccgt cgccgcacac cgtattttta ttcttcagca
     1201 tgatgagaaa gagataacga tggaaagcac agccttacag caggtctttg acacctgtca
     1261 gaataacaaa gcagcatggc tgcaacgcaa aaatgagctg gctgcggccg aacaggaata
     1321 tctgcggctt ctgtccgggg aaggcagaaa cgtcagtcgc ctggacgaat tacgcaatat
     1381 tatcgaagtc agaaaatggc aggtgaatca ggctgccggt cgttatattc gttcgcatga
     1441 agccgttcag cacatcagca tccgcgaccg gctgaatgat tttatgcagc agcacggcac
     1501 agcactggcg gcggcactgg caccggagct gatgggctac agtgagctga cggccattgc
     1561 ccgaaactgt gccatacagc gtgccacaga tgccctgcgt gaagcccttc tgtcctggct
     1621 tgcgaagggg gaaaaaatta attattccgc acaggatagt gacattttaa cgaccatcgg
     1681 attcaggcct gacgcgactt cggtggatga cagccgtgaa aaattcaccc ctgcgcagaa
     1741 catgattttt tcgcgtaaaa gtgcgcaact ggcatcacgt cagtctgtgt aaaattcccc
     1801 gaaaatccgc ccgtttttac tgaaaaaagc catgcatcga taaggtgcat ggctttgcat
     1861 gcgttttcct gcctcatttt ctgcagaccg cgccattccc ggcgcagcct gagcgtgact
     1921 gtgcaactgc attaaaaccg ccctgcaaag cgggcgggcg tggcggggag agcattgcgc
     1981 gccatgccgt gaggataatt attttattaa tattcagctc atgacgtctg taattgggaa
     2041 gaagtgaaaa atgctcatgt aacttatgaa tatttaacgt taaaccgatc aaattctagg
     2101 gtgaaaacat tgcccgcacg aagtcaatgg ggttatgctt aatttttgat catgatacat
     2161 atcccaagat aagaagctaa ggaaagagca tgaatgtagg gcatctcaat ttcttcaagg
     2221 ttaataagtg cggtttatac aaggtaaatg atgacaatac ctatggttta gaacttagcg
     2281 aaacatttga cctcatacag gattgggtgg gaactaaatc tcttgcactc acgataccat
     2341 gggatccaaa agaaaaacca aacagttcaa agtgttattg caaggatatt tataaggatg
     2401 aaaatactgg tgatttctta gtcatgttat ggaaatctga tactgatagt acgggctctt
     2461 tattaggagc tagtgaagac ggtgaaatcg gaagctcatc ggtggttaaa tatactaata
     2521 gctacagagg gaaaaaagtt atctgggggc ggccatgctt ttattgggtt atacctgaac
     2581 ttgaaactat agtatccatt aaattcgacc attctgtttg tgattctgaa ctatttcaag
     2641 actatgtaca ctcatctatt actaatagag ttaaacatag caaaagggtt aaaaataaaa
     2701 ccgagaaagg ttatataagg ctatctaata ccgatgacga agacttatat aaatatatgt
     2761 acaggtttga tatgaagctc agaagcttag aaaccactca tactgagtta ggtaaactaa
     2821 taccaaaaat aacacacata gttcgcaggg aaactattat tataaatcca aatgatgcac
     2881 gagctgattg gttaaagacc tttagcactc tcgttccatt tgtgtccgga aagaaaaatg
     2941 ccaaaacaag acaaattgaa ataaaagcag aagcaaaacc atccttgaat gaagttcaag
     3001 aaatcattga aaagtattcg gctgaggata gagaaaaacg tctttgggac aatgttggat
     3061 ttgctacaga caagggtata acttgggtgg ataaatatag aatgcgtgat attctcaatg
     3121 tcccttctga cgactattca acatattccg ccgcatatat ttacgaacaa atatcacaca
     3181 aaagaaaaga atttatttcc cctatattaa aagaattaaa agcattacaa tctcaaccaa
     3241 gaataaagaa agcatctgga gaggattgaa tattgaaagc tatagcaggc atagccaaat
     3301 acacatggat atacatggcg atattcatta tatcgctgat gattatttcg tcatccgata
     3361 ttaactatgt gtataacgat tttgccaact attgcactct tctcgtatca gtttctggaa
     3421 tggtttttac tattatggga atttggttag cgtttcttta cccaaatgct ttgcagcgta
     3481 ttgtgaaccc caaaactata gaaaccgttg atttcaccga acaactggaa gatacaagaa
     3541 gattagaagc aatagttgga agtgttctaa aatcatcttt cgttatgatt actttacttg
     3601 ttgtatactt agttaaacta gtgtttttca aaagtcatct ttatttagag agccaagatg
     3661 taataaaaaa cacatcttta gcattaatta ttgctatttc gtacatgcag attgaggcag
     3721 taattagcgt aatacttgca aatgttatgt ttatcaatga tttacataac aagcgagagc
     3781 aaagagacat tgatgatcaa gtatgaggga aaaatgaaaa agaatatacg tgttatacct
     3841 aaaaatattc atagcaagtt aagaaggctt ggtaatacag ttgttgctgg cacttctatt
     3901 gcatttacgg aaaaccaatt aaaaagtgga gcattagagc accttggaat atattttgat
     3961 aatggtgtta atgcttatga gacgtcggtc ataccagatc cattgcaggg taaatattca
     4021 cttaaaaatg tttttggtga agaaattgtc agaaaagacc tcccaaaaga aactcattac
     4081 actgaaattg agtcacctaa ctggggtgat agctctaatg gtacacatac tgttagatta
     4141 ccttacgaga aatatcccag agacattata ccaccaacac tcatagccat tgaaattaat
     4201 cacaaacagc cctcggacag ccatttcata tttaacttca gagtaactag aattttagac
     4261 aaacaatcaa ataagtttga tgatgaatta ttatttgatt taaatcttct tcaagaaaat
     4321 cttggtaaat gtggaattga ggatgctgat aaacccatat cgacttatgc tgatacactt
     4381 atagtctcat gggaaatatt tccaccaggt agtaaagaag aaactttagc aagaattttt
     4441 agaggtaaaa atatcacttc cgacaagaaa aatgtcgctg aaaatagata tgacttcttc
     4501 atgagtcttg aacctaaaaa aatcgtcaca ggaaacagca cgttcagtaa ttatattgga
     4561 gctatgctgg aggacgattt ggttgtattt gaaaacatag aatatggtaa tgctatatat
     4621 atcctctatg ataactggga tgatatcagc aagctatctc gaattgactt attatcagga
     4681 agagcaggaa gtaatttcga tagaataatc catagtggca attggaaaga tgaagtacga
     4741 aaaaaagttg ctgctggaag attatgatca aagtgtttaa tacagaaata aacgcctata
     4801 tattataggc gtttattttc actatcgact tttgtttatt ttagcaaaat cataaggtgt
     4861 tattgttttg cccttatttg cttggagata atcagcccac cattgaacca tcaatcgacg
     4921 ttcatccaaa tgttcagagg tgtgaatata agcagcacgt acattattac gctctgaatg
     4981 gctcaactgt cgttctatag catcttcact ccataatccg gactcgccca acgcaccacg
     5041 ggccatcgtt ctaaacccgt gcccgcaaac ttcggttttc gtgtcatagc ccatcgcacg
     5101 caatgcgcta tttaccgtgt tttcgctcat aaccttagtt gcgtcatgat cacccggaaa
     5161 aagcagctct ttatctccac taatctgctt taactggttt agcaaaatca tcgcctgccg
     5221 actaagctga acgatatgct cctctttcat cttcatgcca cggtacgagt aacgcacacc
     5281 tttaatttct tctcgttttg caggtactcg ccagagagat ttatcgaagt cgaactcatc
     5341 ccaacgcgcg aaacgtaact cactggaacg cacaaaagtt agtaaggaaa gctcaaccgc
     5401 gatccgcgtc attacacggc cacgatatgc agcaagacga gcaagaaact cagggaaccg
     5461 gctggagggc aaagcggggt aatgtcgcgc tttggttgtc gatagcgcac cggccatatc
     5521 actggctgga tttgagtcga tgtaatcgtt ctgtactgca taacgcataa tagccgtgac
     5581 acgctgctga aggcgctgtg caacgtcatg cttaccactg gcatcaactt ttttaatcgg
     5641 ggctaatagg tggctagttt tgagctgacg gatgtcagat gaaccaatat gaggaaagat
     5701 ataaagctca agataacgta gaacgcgtga tcggtgatct tcactccagc gcttattact
     5761 agcatgccat tcacgagcga tagtttcgaa agaatatgcc cccgaattct cggcctgagc
     5821 ttctttctgt tcggcttttg gatcaatgcc ctgcgctaac agctttttag cttgatcgcg
     5881 ctttgctctt gcctgagcaa gcgtcacagt aggccaaaca ccaaacgcga gacgatcctc
     5941 ttttttgtca gagggacgtc tgtatttcat gcgccagtat ttggaacctt tggccgaaac
     6001 ctcaagatac aaaccaccac catcggccat tttgtaggtt ttgtcttttg gctttgcggt
     6061 ctcgacctgt ctggcgttga gcttcatttg ggggcacatt tctaatcgaa gttaagatgc
     6121 ccccaattat gcccccaatg atatccggat ttcaacggac aacctcggaa gacgcaggac
     6181 gaaaaaatcg ctgcaagcat tgattttaaa gggatatttg gactttctcg gatggtcttg
     6241 gaagtaagaa tggtgccgaa ggccggactc gaaccggcac gtatttctac ggttgatttt
     6301 gaatcaactg cgtctaccga tttcgccact tcggcactga agaggtatgc ggaaaacgtt
     6361 gtggattata cctgtcgcac gccaccatgc aagcgccagc ttgctcgcac ctcgctaagt
     6421 gctgaaattt tcagcattgc acacagcgat gtgtaacctt tgtcacactc caggcacccc
     6481 gccctgccat gctctacact tcccaaacaa caccagagaa ggaccaaaaa atgtcgatga
     6541 taaaaagcta tgccgcaaaa gaagcgggcg gcgaactgga agtttatgag tacgatcccg
     6601 gtgagctgag gccacaagat gttgaagtgc aggtggatta ttgcgggatc tgccattccg
     6661 atctatcgat gatcgacaat gaatggggat tttcacaata tccgctggtt gccgggcatg
     6721 aggtgattgg gcgcgtggtg gcactcggga gcgccgcgca ggataaaggt ttgcaggtcg
     6781 gtcagcgtgt cgggattggc tggacggcgc gtagctgtgg tcactgcgac gcctgtatta
     6841 gcggtaatca gatcaactgc gagcaaggtg cggtgccgac gattatgaat cgcggtggct
     6901 ttgccgagaa gttgcgtgcg gactggcaat gggtgattcc actgccagaa aatattgata
     6961 tcgagtccgc cgggccgctg ttgtgcggcg gtatcacggt ctttaaacca ctgttgatgc
     7021 accatatcac tgctaccagc cgcgttgggg taattggtat tggcgggctg gggcatatcg
     7081 ctataaaact tctgcacgca atgggatgcg aggtgacggc ctttagttct aatccggcga
     7141 aagagcagga agtactggcg atgggtgccg ataaagtggt gaatagccgc gatccgcagg
     7201 cactgaaagc cctgtcgggg cagtttgatc tcattatcaa tactgtgaac gtcagcctcg
     7261 actggcagcc ttattttgag gcgctgacct atggcggtaa tttccatacg gtcggtgtgg
     7321 ttctcacgcc gctgtctgtt ccggccttta cgttaattgc gggcgaccgc agcatctctg
     7381 gttctgctac cggcacgcct tatgagctgc gaaagctgat gcgctttgcc gcccgtagca
     7441 aggttgcgcc gaccaccgaa ctgttcccga tgtcgaaaat taacgatgcc atcaagcatg
     7501 tgcgcgacgg taaggcgcgt taccgcgtgg ttctgaaagc cgacttctga tccctttgcc
     7561 gggtgcctgt cgcccggcac ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt
     7621 agcggtgaag agtgcgcagc cgttgcctat actccaggca ggaaactgga ggaaatctca
     7681 tgagcgaacc cctattaatt gcccgcacgc cggacacaga acttttttta ctgccgggaa
     7741 tggctaaccg tcacgggctg attactggcg caacggggac gggtaaaacc gttacgctgc
     7801 aaaaactggc agagtcattg tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag
     7861 gcgatctgac cggtatcgcg caggcaggaa cggcgtcgga aaaactgctc acaaggctta
     7921 aaaatatcgg cgtcaatgac tggcaaccgc atgctaatcc ggtggtggtg tgggatatct
     7981 ttggcgagaa aggccatccg gtgcgggcga cggtttcaga cctggggccg ctgttgctgg
     8041 cgcggctgtt gaatctcaac gatgtgcaat ctggcgtgct gaatatcatc ttccgtattg
     8101 ctgacgatca gggattgttg ctgctcgact ttaaagatct gcgggcgatt acccagtaca
     8161 tcggcgataa cgccaaatct ttccagaatc agtacggtaa tatcagtagc gcatcggttg
     8221 gtgccatcca gcgcggttta ctgtcgctgg aaaagcaagg tgcggcgcat ttctttggcg
     8281 agccgatgct ggatatcaaa gactggatgc gcaccgatac caacggtaaa ggcgttatca
     8341 atatcctcag cgccgagaag ctttatcaga tgccgaaact gtacgccgcc agcctgctgt
     8401 ggatgctttc agagttgtat gaacaattgc cggaagcggg cgatctggag aaaccaaaac
     8461 tggtgttttt cttcgacgag gcacatctgc tgtttaacga tgcaccgcag gtactgctgg
     8521 ataagattga gcaggtaata aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt
     8581 cgcaaaaccc gtctgatatt ccggataatg tgctcgggca gctcggtaat cgcgttcaac
     8641 acgctttgcg ggcttttacg cccaaagatc agaaagcggt aaaagctgcg gcgcaaacca
     8701 tgcgggccaa tccggcgttt gataccgaaa aggcaatcca ggaactgggg accggcgagg
     8761 cgttaatctc gtttctcgat gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga
     8821 ttgcgccttg ttcgcggatg ggaccggtga cggaagatga gcgcaatggc ttgattaacc
     8881 actccccggt gtatggcaag tacgaggatg acgtggaccg ggaatccgcc tatgagatgt
     8941 tgcaaaaagg ctttcaggcc agtaccaagc agcaaaataa tcctcccgcg aaagggaaag
     9001 cggtggcggt ggatgacggt attcttggtg gattgaagga tattttgttt ggcactaccg
     9061 gaccacgcgg cgggaagaaa gatggtgtgg tgcaaacaat ggcgaaaagc gccgcccgcc
     9121 aggtgacgaa tcagattgta cgtgggatgt tggggagttt gctgggggga agaagaaggt
     9181 aagtcaggcg agatttgtct ttgcctgatg cgacgctggc gcgtcttatc atgcctacac
     9241 cactgcaata tattggattt tcatgatttt tcaggcatga acgaagcaca ctttgctaac
     9301 aataataaag ggagctttcg ctccctttat tcgttcattc ggttacgatt ttctcattaa
     9361 cagccacagg ctgattaaga agaagctggc gcttggcaac agtgcgccga tgatcggcgg
     9421 gatgccataa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     9481 gaagctgata ccggtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     9541 gaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     9601 catattgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     9661 gtgcaaaccg ctgattgaga gtgcatccgg gtccagcgcc accacgccca gtttgtccgg
     9721 cgtgaggttg gttttccagg tgccgctcac cgtctgcgaa ccggtgatct gtttcggatt
     9781 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     9841 cgcagtagcg gcatagcgta cggattgcag acgacgattc tcgttaaagg cataaatact
     9901 gatgccacct aactcttcgt cacctttaac ccgctcaatg taaacgaagt tgttgccatc
     9961 tttcgcccat aagccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
    10021 gtagttacgc gccatctgct cgccctgcgg cgcgacccat tcaccaatcg ccatcgtcag
    10081 caagaccagc ggaatggcgg ttttcatcac cgacagcgca acctgcatac gggtgaaacc
    10141 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
    10201 cccaagcaga gccgccatcg ggaagaatgt ttgcacatct ttcggcacgc tcagcaaggt
    10261 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
    10321 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
    10381 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
    10441 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
    10501 gccaaagcca gataaatcag gttaacggtc cacatccaca gcgtcgggtc cagcttacct
    10561 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
    10621 gctggcagca tcgatagtac gcgtccctga cgcgggttaa ccacgctcag cggtacgacc
    10681 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcgg
    10741 gcacgatcgg tgtcagtgtt ccacaatgta cgcatgtcca tctggtcggt atcgttcggg
    10801 tcgagcgcta ccgcctggtg accaatgatc gcctgataat cctggaagtc cgtaatgcgg
    10861 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
    10921 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
    10981 ttaccttttg gtcgaatttg cgcgaggaac acatctttga aatcgctgcc gtcaacgctt
    11041 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgccctgg cgccagcgcc
    11101 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
    11161 ggtcccgccc acatcacgtt aaccgccgcg acgattgcag tgaataccgc aaggatcatt
    11221 gccgctttca ccagaaccgc tttgctcagg ccgcaggcat gcattaccgt aatttcactt
    11281 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
    11341 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
    11401 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
    11461 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
    11521 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
    11581 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
    11641 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
    11701 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
    11761 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
    11821 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
    11881 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgtcgggg
    11941 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
    12001 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
    12061 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcaatgg aagcggtctg
    12121 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
    12181 cgagacggca aaagagacgc tctacagttt cgatcagctg aaaacgaaca agagcgaacc
    12241 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
    12301 tgagcgcgcg atcctgcacg gtctggcgat tgccgccggg attaaagcag caaaagatct
    12361 cggcaatatg ccgccgaata tctgtaacgc cgcttacctc gcttcacaag cgcgccagct
    12421 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
    12481 gctggggatg cattcctatc tggcggtcgg tcagggttcg caaaacgaat cgctgatgtc
    12541 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
    12601 aggtttaacc ttcgactccg gcggtatctc gatcaagcct tcagaaggca tggatgagat
    12661 gaagtacgat atgtgcggtg cggcagcggt ttacggcgtg atgcggatgg tcgcggagct
    12721 acaactgccg attaacgtta tcggcgtgtt ggcaggctgc gaaaacatgc ctggcggacg
    12781 agcctatcgt ccgggcgatg tgttaaccac catgtccggt caaactgttg aagtgctgaa
    12841 taccgacgct gaaggccgcc tggtactgtg cgacgtgtta acttacgttg agcgttttga
    12901 gccggaagcg gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
    12961 tcacattacc ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
    13021 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
    13081 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
    13141 aggttgcttc ctgtcgcgct ttacccgtaa gtacaactgg gcgcacctgg atatcgccgg
    13201 aaccgcctgg cgttctggta aagcaaaagg cgccaccggt cgtccggtgg cgttgctggc
    13261 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taattgcgtc aggcaaggct
    13321 gttattgccg gatgcggcgt gaacgcctta tccgacctac acagcactga actcgtaggc
    13381 ctgataagac acaacagcgt cgcatcaggc gctgcggtgt atacctgatg cgtatttaaa
    13441 tccaccacaa gaagccccat atatgaaaaa cgcgacgttc taccttctgg acaatgacac
    13501 caccgtcgat ggcttaagcg ccgttgagca actggtgtgt gaaattgccg cagaacgttg
    13561 gcgcagcggt aagcgcgtgc tcatcgcctg tgaagatgaa aagcaggctt accggctgga
    13621 tgaagccctg tgggcgcgtc cggcagaaag ctttgttccg cataatttag cgggagaagg
    13681 accgcgcggc ggtgcgccgg tggagatcgc ctggccgcaa aaacgcagca gcagcccgcg
    13741 cgatatattg attagtctgc gaacaagctt tgcagatttt gccaccgctt tcacagaagt
    13801 ggtagacttc gttccttatg aagattctct gaaacaactg gcgcgcgaac gctataaagc
    13861 ctaccgcgtg gctggtttca acctgaatac ggcaacctgg aaataatgga aaagacatat
    13921 aacccacaag atatcgaaca gccgctttac gagcactggg aaaagcaggg ctactttaag
    13981 cctaatggcg atgaaagcca ggaaagtttc tgcatcatga tcccgccgcc gaacgtcacc
    14041 ggcagtttgc atatgggtca cgccttccag caaaccatca tggataccat gatccgctat
    14101 cagcgcatgc agggcaaaaa caccctgtgg caggtcggta ctgaccacgc cgggatcgct
    14161 acccagatgg tcgttgagcg caagattgcc gcagaagaag gtaaaacccg tcacgactac
    14221 ggccgcgaag ctttcatcga caaaatttgg gaatggaaag cggaatccgg cggcacgatt
    14281 acccgtcaga tgcgccgtct cggcaactcc gtcgactggg agcgtgaacg cttcacgatg
    14341 gacgaaggcc tgtccaatgc ggtgaaagaa gttttcgttc gtctgtataa agaagacctg
    14401 atttaccgtg gcaaacgcct ggtaaactgg gatccgaaac tgcgcaccgc tatctctgac
    14461 ctggaagtgg aaaaccgcga atcgaaaggt tcgatgtggc acatccgcta tccgctggct
    14521 gacggcgcga aaaccgcaga cggtaaagat tatctggtgg ttgcgactac ccgtccggaa
    14581 accctgttgg gcgataccgg cgtggccgtt aacccggaag atccgcgtta caaagacctg
    14641 attggcaaat atgtcattct accgctggtt aaccgtcgta ttccgatcgt tggcgacgaa
    14701 cacgccgaca tggaaaaagg caccggctgc gtgaaaatca ctccggcgca cgactttaac
    14761 gactatgaag tgggtaaacg tcacgcgctg ccgatgatca acatcctgac ctttgacggc
    14821 gatatccgtg aaagcgccca ggtgttcgat accaaaggta acgaatctga cgtttattcc
    14881 agcgaaatcc cggcagagtt ccagaaactg gagcgttttg ctgcacgtaa agcagtcgtt
    14941 gctgcggttg acgcgctcgg cctgctggaa gaaattaaac cgcacgacct gaccgtgcct
    15001 tacggcgacc gtggcggcgt agttatcgaa ccgatgctca ccgaccagtg gtacgtgcgt
    15061 gccgatgtac tggcgaaacc ggcggttgag gcggttgaga acggcgacat tcagttcgta
    15121 ccgaagcagt acgaaaacat gtacttctcc tggatgcgcg atattcagga ctggtgtatc
    15181 tctcgtcagc tgtggtgggg tcaccgtatc cctgcatggt acgacgaagc aggtaacgtt
    15241 tatgttggcc gcaacgaaga agaagtgcgc aaggaaaata acctcggtgc cgacgttgcc
    15301 ctgcgtcagg acgaagacgt tctcgacacc tggttctctt ctgcgctgtg gaccttctct
    15361 accctaggct ggccggaaaa caccgacgca ctgcgccagt tccacccaac cagcgtgatg
    15421 gtatctggtt tcgacatcat tttcttctgg attgcccgca tgatcatgat gaccatgcac
    15481 ttcatcaaag atgaaaatgg caaaccgcag gtgccgttcc acaccgttta catgaccggc
    15541 ctgattcgtg acgacgaagg ccagaagatg tccaaatcca agggtaacgt tattgacccg
    15601 ctggatatgg ttgacggtat ttcgctgccg gaactgctgg agaaacgtac cggcaatatg
    15661 atgcagccgc agctggcgga caaaatccgt aagcgcaccg agaaacagtt cccgaacggc
    15721 attgagccgc acggcaccga tgccctgcgc ttcaccctgg cggcgctggc ttctaccggt
    15781 cgtgacatca actgggatat gaaacgcctg gaaggctacc gtaacttctg taacaagctg
    15841 tggaacgcca gccgctttgt gctgatgaac acggaaggtc aggattgcgg cttcaacggt
    15901 ggtgaaatga cgctgtcgct ggcggaccgc tggattctgg cagagttcaa ccagaccatc
    15961 aaagcgtacc gcgaagcgct ggacagcttc cgcttcgata tcgctgcagg cattctgtat
    16021 gagttcacct ggaaccagtt ctgcgactgg tatctggagc tgactaagcc ggtaatgaac
    16081 ggtggaaccg aagccgaact gcgcggtact cgccatacgc tggtgactgt actggaaggt
    16141 ctgctgcgcc tcgcgcatcc gatcattccg ttcatcaccg aaaccatctg gcagcgtgtg
    16201 aaagtacttt gcggtatcac tgccgacacc atcatgctgc agccgttccc gcagtacgat
    16261 gcatctcagg ttgatgaagc cgcactggct gacaccgagt ggctgaaaca ggcgatcgtt
    16321 gcggtacgta acatccgtgc agaaatgaac atcgcgccgg gcaaaccgct ggagctgctg
    16381 ctgcgtggtt gcagcgcgga tgcagaacgt cgcgtaaatg aaaaccgtgg cttcctgcaa
    16441 accctggcgc gtctggaaag catcaccgtg ctgcctgccg atgacaaagg tccggtttcc
    16501 gttacgaaga tcatcgacgg cgcagagctg ttgatcccga tggctggcct catcaacaaa
    16561 gaagatgagc tggcgcgtct ggcgaaagaa gtggcgaaga tcgaaggcga aatcagccgt
    16621 atcgagaata agctggcgaa tgaaggcttt gtcgcccgcg caccggaagc ggtcatcgcg
    16681 aaagagcgtg agaagctgga aggctatgca gaagcgaaag cgaagctgat tgaacagcag
    16741 gctgttatcg ccgcgctgta atcaaaacac agtgatgaaa acgaaggccg gagcatgctc
    16801 cggccttttt tatctcttac agaaatggta aagaaggcat aattccccgt gaaatactgg
    16861 ctaagaagcc tttatctggt tgttcttctt tattgattaa agga
//
LOCUS       DADLTD010000084        16618 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLTD010000084 DADLTD010000000
VERSION     DADLTD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.084"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..243)
                     /locus_tag="JN701_002149"
     CDS             complement(<1..243)
                     /locus_tag="JN701_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7364597.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGL"
     gene            350..646
                     /locus_tag="JN701_002150"
     CDS             350..646
                     /locus_tag="JN701_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7364598.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(780..1205)
                     /gene="arsC"
                     /locus_tag="JN701_002151"
     CDS             complement(780..1205)
                     /gene="arsC"
                     /locus_tag="JN701_002151"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY7364599.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1218..2507)
                     /gene="arsB"
                     /locus_tag="JN701_002152"
     CDS             complement(1218..2507)
                     /gene="arsB"
                     /locus_tag="JN701_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY7364600.1"
                     /translation="MLLAGAIFVPTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTLIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2562..2915)
                     /gene="arsR"
                     /locus_tag="JN701_002153"
     CDS             complement(2562..2915)
                     /gene="arsR"
                     /locus_tag="JN701_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY7364601.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3654..3737
                     /locus_tag="JN701_002154"
     CDS             3654..3737
                     /locus_tag="JN701_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY7364602.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3791..5143)
                     /gene="gorA"
                     /locus_tag="JN701_002155"
     CDS             complement(3791..5143)
                     /gene="gorA"
                     /locus_tag="JN701_002155"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY7364603.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5215..6057)
                     /locus_tag="JN701_002156"
     CDS             complement(5215..6057)
                     /locus_tag="JN701_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY7364604.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6260..8302
                     /gene="prlC"
                     /locus_tag="JN701_002157"
     CDS             6260..8302
                     /gene="prlC"
                     /locus_tag="JN701_002157"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY7364605.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8310..9062
                     /gene="rsmJ"
                     /locus_tag="JN701_002158"
     CDS             8310..9062
                     /gene="rsmJ"
                     /locus_tag="JN701_002158"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY7364606.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9111..10580)
                     /gene="dtpB"
                     /locus_tag="JN701_002159"
     CDS             complement(9111..10580)
                     /gene="dtpB"
                     /locus_tag="JN701_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAY7364607.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLANPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10898..11332)
                     /gene="uspA"
                     /locus_tag="JN701_002160"
     CDS             complement(10898..11332)
                     /gene="uspA"
                     /locus_tag="JN701_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY7364608.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11723..12058
                     /gene="uspB"
                     /locus_tag="JN701_002161"
     CDS             11723..12058
                     /gene="uspB"
                     /locus_tag="JN701_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY7364609.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12302..13801)
                     /gene="pitA"
                     /locus_tag="JN701_002162"
     CDS             complement(12302..13801)
                     /gene="pitA"
                     /locus_tag="JN701_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY7364610.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14033..15235
                     /locus_tag="JN701_002163"
     CDS             14033..15235
                     /locus_tag="JN701_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7364611.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15550..16602)
                     /locus_tag="JN701_002164"
     CDS             complement(15550..16602)
                     /locus_tag="JN701_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HAY7364612.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGYVIFGIGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILIYLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4267 a         4235 c         4314 g         3802 t
ORIGIN      
        1 caatccacgt aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag
       61 agcagccagg agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc
      121 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
      181 ctcgacggcg agaaacacaa acatctctgc ggcatcttgc agcatcgcaa gccaactact
      241 catacgtaac ctcattggat aaatctatat ttccagaata atcgaaatga aattcagatc
      301 aagatatttc tggtattatc gaaatatcaa ccagacagag gggtgtcata tgcagttgga
      361 agaagtcgct aaagcgctga aagagctagg tcacccgacc cgtttgttca tatttaaaca
      421 tcttgtcaaa gccggggaac aagggttgcc ggtcggtgag ttgcaaaagc agcttgggat
      481 acctggctcc acactcagcc accatatatc agcactggta tcggtaggac tggtcaaaca
      541 aaaccgtgag agtcggacgc tgatgtgtgt ttcgcaatac gcaatgctgg aagcgataat
      601 tgaattctta cgcgaggagt gctgcgtaaa tagcaaaatt gcctgatgcg ctacgctcta
      661 catatttcat gccatatatt gaatttgcac ggttttgtag gtcggataag acgcgttagc
      721 gtcgcatccg acataaacaa agcgcacttt accaactatc ggcagtggat atcgccgctt
      781 tatttcaggc gcttacccgc ttcatcaacc actttctcgc catcttcctt ggagaatgcg
      841 cctttttgcg catctggcag aatttccagc accacttctg aagggcggca caggcgagtt
      901 cccagcggcg tcaccacaat cgggcgatta atcagaatcg ggtgctgaag cataaagtcg
      961 attaaccgat cgtcagtaaa tttatcttcc gcaaggccca gctcctcata cggttcgacg
     1021 tttttacgca gcagcgcgcg tacggaaatc cccatatcgg caatgagttt gaccagttca
     1081 tcgcgcgttg gcggagtttc cagataatgg ataatggtcg gttctgtgcc gctgttgcgg
     1141 atcatctcca gcgtattacg cgacgtgccg caggccgggt tgtgataaat ggtaatgttg
     1201 ctcatatcag tatctcatta caaagtgaaa gagagacgta gcgccagcgc agccagcgtc
     1261 acaaacagca caggcagggt catgataatc cctgtacgga agtaatatcc ccagctgata
     1321 gtcatattct tctgcgaaag tacgtgcagc cagagtagtg tagccaggct accaattggg
     1381 gtaattttcg gtcccaaatc gcagccaatc acattggcat aaaccatcgc ttctttgata
     1441 acgccagatg ccgtgctgcc atcaatggac aacgcgccaa ccagtaccgt cggcatattg
     1501 ttcataatag aagagaggaa ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg
     1561 ccgttatccg ccagcacgtt gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc
     1621 aggccataaa ccaccagata catgccgagc gagaagatga caatttgcca gggggcaccg
     1681 cgcaggactt tacccgtatt aatcgcatga ccgcgtttag cgacgacaaa taatatcagc
     1741 gcgcccacag ctgcaatggc gctcaccgga atgccgagcg gttccaggac gaaaaatccc
     1801 accagcagaa gcagtaaaac aacccagcca gttttgaacg tagcaggatc tttgatcgct
     1861 tctgcgggag atttcagcag cgccatatcg tagttctgcg gaatatcttt gcgaaaatag
     1921 agatgtagca tcaccagcgt ggcaacaatc gcggcgatat ccaccggcac catcaccgag
     1981 gcgtattcgc gaaagccgag gccaaagaaa tcagcggaaa cgatattcac caggttggag
     2041 acaataagcg gcaggctggc ggtatcggca atgaatccgg ccgccatcac gaacgccagc
     2101 gtcgtgcctt tactgaatcc taaagccagc agcatggcga tgacaatcag cgtcaaaata
     2161 agcgctgcgc catcattggc aaacagggcg gcaacggcag caccgagcag gacaatccag
     2221 gtaaacagca agcgaccacg accattaccc cagcgtgaga cgtgcagcgc cgcccattca
     2281 aaaaagccgg actcatccag cagcaggctg atgataatga cggcgataaa cgcagccgtc
     2341 gcgttccaga cgatattcca caccaccgga atatcacccg gatggaccac gcccgtaact
     2401 aacgccagta ctgcgccgag cgttgcactc cagccgatgc ctaaaccttt cggctgccag
     2461 ataaccaata caatggtcgg gacaaagata gcgcctgcca gtaacataat gcctcctgga
     2521 taaaacacat gcgaaaatac atatgtgttt agctaaattt tttaactgca aatgttctta
     2581 ctgtctgcgg aacagttttg tcgagccagg ttgcggacaa ttgcctgaat cttttcctgt
     2641 tcacatcgcc aggcttcatc aattattttc gccgcccatg ctggaatatg cggcgataag
     2701 cggtaatgaa cccatttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2761 gccaggtggc gggagatctt gggctgcgac tggtcgagag cagtgcagag atcgcagacg
     2821 cataactctc ccagttcgct gagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2881 attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt gttgcaggta
     2941 gtgtctctct tcgaagcgga taagtcaaaa acatatatga tttaacgaat gtgtaagtgc
     3001 agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaagga ggtctgaatc
     3061 aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggct
     3121 ctggctggag tgagagagtt cttatctaac agctcaatac tattagccag tggctaacat
     3181 tgcaagaatt agcgatttct tcagctggcg gagggagcac ttcggtatat atcgcagtga
     3241 cgatttatat actttcactg ggtcatcgtc atattaagcc ttgtccgaat ccggatgtaa
     3301 aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggct ctgactggag
     3361 tgagatagcc atcatctaac gcatcaatgc taataccata ctgaaactat tgcaaggacg
     3421 tgctggtttt ataacctgca tgtactgtat gattatccag ttagctctga ggcattttca
     3481 ctctggcaat gcgcataaac gctttcaaag tcctggtcag aagtacgggt ggtgccgtta
     3541 actgatgctc tggccggagt gagagagttc ttatctaaca atgagacatg cgccgtgaca
     3601 ggcagtggat gagtaagcgg atgcattctc actccatcgc atggagaaaa cgggtgattg
     3661 ataaagcaat catcgttcta ggggcgttaa ttgcgctgct ggaactgatc cgctttctgc
     3721 ttcagcttct gaactgatag cggaaacgta attaagggct aagagcacac tactcttagc
     3781 cctttaacat ttaacgcatt gtcacgaact cttctgccgc cgttgggtga atggcgacgg
     3841 tattgtcgaa gtcttttttg gttgccccca tcttcagcgc caccgcgaag ccctgcaaca
     3901 tttcgtccat accaaagcca atgccgtgaa taccgacaat cttctcttcc gatccaacgc
     3961 acaccagctt catgcggcac ggctggcggt gagtggtgac ggcggtatac atcgcggtga
     4021 aagaggattt atacactttc acctgatcgt cgccatactg ctcgcgcgcc tgcggttccg
     4081 ttaaaccaac agtaccaatc ggcggatggc tgaagaccac ggtcggaatg ttgctgtaat
     4141 ccagatgctc atccggcttg ttattaaaca ggtgttcaga gagacggcga cccgctgcaa
     4201 ctgccaccgg tgtcagctcc actgcacccg tgttatcgcc caccgcgtaa ataccttcaa
     4261 tgttggtgtt ttgatattta tcgacgacga tatagccttt ttcgttggtt ttaacgccag
     4321 cggcttcgag gttgatgttg tcattggcag gctcgcgacc aatcgcccaa atcaggcaat
     4381 ccaccgtttc actgcgacca tcttccagct ccagcgtcag gctaccatcg gcatttttca
     4441 ctaccgcttt cgggatggcg ttggtgtgca gctgcgggcc ttcggcgttc atcacttcga
     4501 ccagcgtttc ggaaatcatc gggtcgaagc tgcgcagcgg cgcatgttta cgcacaaaca
     4561 gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag ctcaacggcg atgtaacccg
     4621 cgccaacaac cgccacgcgc tctggcaaag caggaagggc gaagaagcca tcagaatcaa
     4681 taccgtattc cacgcccgga atatccgggt ggctcggacg accgcctgtg gcgatcagaa
     4741 tatgatcggc cgtgatggtt tcgccgttta cctccagcat tttggcatca acgaagcggg
     4801 caaagccttt gattacatca acgttatttt taccgagcac gttttcatag gaagtatgaa
     4861 tacggtcgat ataggcggta cggctggcga tcaacgtttc ccagttgaac ttgttgatgg
     4921 tggtatcgaa accataatca gggccgtaca tatggatcgc ttcacggatt tgcgccgcgt
     4981 gccacatcac ttttttcggc acgcagccga cgttcacgca ggtgccgccc agctctttgg
     5041 cttcaatcag tgcgcatttc tggccgtaca tagccgcgcg gttgatggag gctataccgc
     5101 cgctgccgcc gccgatggcg atgtaatcat agtgtttagt catgacagag tgtccttatc
     5161 gttgattacc gcgattgtag cgcgagacgt aataggtgcc agcaatggct gcaattactc
     5221 cggcacgatc cagcttacgg tggcgtgccc ggtgcctgcc ggaaccagtt tgctgtgcag
     5281 ccacggcagc acgttattca tctgctgttc cagtttccac ggcgggttaa tcacaatcat
     5341 gccggaagcg gtcatgccac ggcgatcgct gtctggcagt accgccagtt caatttgcag
     5401 aattttgcga ataccggtcg cttccagatc gtggatcatg cgcttaattt gctgacgcag
     5461 caccaccgga taccacagtg cgtaagtacc agtggcgaaa cgtttgtaac cttctgctat
     5521 cccgctgacc accgcctgat agtcggtttt catttcatac ggcgggtcga taaggattaa
     5581 accacggcgg gaaaccggcg gcagtttggc cttaagctgc tggaaaccat cggctttttc
     5641 gacgcgcgca cggctgtctt tctgaaattc agaacgcagc aacgggtaat cgctcgggtg
     5701 cagttcggtc agttgcaggc tgtcttgttc gcgcagtagc tggcgagcaa tcaatggcga
     5761 accggggtag taacgcaact gaccgctacg gttgaagtgt tttaccacat tgatgtacgc
     5821 ctccagttct gcgggcaaat cgtcctgctg ccagatacgg gcgatgcctt cgagatattc
     5881 gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc ccggcccctg cgtgggtgtc
     5941 gagatagaga aacggtttat ctttctcttt cagcgactcg atgatcaggc tctgaacggt
     6001 atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg tggcgataac tgagcatggg
     6061 taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg gcgcacaaaa gcgtatcagg
     6121 acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac gtttctcatt gaaattcact
     6181 acacttaacc ccatgctaca cacattatgt aaagcgcctg ttgagcgctt ccttaacctc
     6241 tttaaccagg actgcgcgaa tgacgaatcc gttactgact ccctttgaat tgcctccgtt
     6301 ttctaaaatt ctcccggaac atgtcgttcc agccgtgact caggcgctga acgattgccg
     6361 cgaaaatgtg gagcgcgtag tagcgcaagg ggcaccgtac acctgggaaa atctctgcca
     6421 gccgttggcg gaagtggacg atgtgttggg gcgtatcttc tccccggtca gccacctgaa
     6481 ctcggtgaaa aatagcccgg aactgcgtga agcctacgag caaaccctgc cgctgctgtc
     6541 ggaatacagc acctgggtag ggcaacatga agggctgtat aaagcgtacc gcgacctgcg
     6601 cgatggcgat cattacgcca cgctgaacac ggcgcagaag aaagcggttg ataacgccct
     6661 gcgtgatttt gaactctctg gcataggtct gccgaaagag aaacagcagc gttacggcga
     6721 aattgcgacc cgtctttctg aactgggcaa ccagtacagc aacaacgtcc tcgatgcgac
     6781 aatgggctgg accaaactcg ttaccgacga agcggagctg gcggggatgc ctgaaagcgc
     6841 gctggctgcg gcaaaagccc aggccgaagc gaaagagctg gaaggctacc tgctgacgct
     6901 ggatatccca agctacctgc cggtaatgac ctactgcgac aaccaggcct tgcgtgaaga
     6961 gatgtatcgc gcttacagca cccgcgcctc cgatcaaggc ccgaacgccg gtaaatggga
     7021 taacagcaag gtgatggaag agatcctcgc tctgcgtcac gaactggcgc aactgctggg
     7081 ctttgaaaac tacgccttta aatcccttgc cactaaaatg gcagaaaacc cgcagcaggt
     7141 gctggatttc ttaaccgatc tggcaaaacg cgcgcgtccg caaggcgaaa aagagctggc
     7201 acaactgcgc gccttcgcca aagcggaatt tggcgtcgat gagttgcagc cgtgggatat
     7261 cgcgtactac agcgaaaaac aaaaacagca cctctacagc atcagtgacg aacagctgcg
     7321 tccgtacttc ccggaaaaca aagcggttaa cggcctgttt gaagtggtga aacgtattta
     7381 cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg catccggatg tacgtttctt
     7441 cgaactgtat gacgagaaca acgaactgcg cggcagcttc tacctcgatc tgtatgcccg
     7501 tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta ggccagatgc gtaaagctga
     7561 cggttctctg caaaaaccgg tcgcgtattt gacttgtaac ttcaaccgcc cggtaaatgg
     7621 taaaccggcg ctgttcactc acgacgaagt gatcaccctg ttccacgagt tcggtcacgg
     7681 cctgcaccat atgctgaccc gcatcgaaac cgctggtgtt tccggtatca gcggtgtgcc
     7741 gtgggatgcg gtcgaactgc cgagtcagtt tatggaaaac tggtgctggg agccggaggc
     7801 gctggcgttt atctctggtc actatgaaac cggcgaaccg ctgccgaaag agttgctgga
     7861 taaaatgctg gcggcgaaga actaccaggc ggcgctgttt attctgcgcc agctggagtt
     7921 cggcctgttc gatttccgcc tccatgccga gttccgcccg gatcagggtg cgaaaatcct
     7981 cgaaactctg gcagaaatca agaaactggt tgccgtagta ccgtctccat cctggggccg
     8041 tttcccgcac gctttcagcc atattttcgc cggtggttat gccgcaggtt actacagcta
     8101 cctgtgggct gacgtactgg cggcagatgc cttctcgcgc tttgaggaag agggcatttt
     8161 caaccgtgaa accgggcagt cgttcctcga caacattctg agccgtggcg gttcagaaga
     8221 gccgatggat ctgttcaaac gcttccgtgg tcgtgaaccg cagctggatg cgatgctgga
     8281 gcattacggc attaagggct gatcattcag tgaaaatctg cttaattgat gaaacaggcg
     8341 ccggagacgg cgccttatct gttctggcgg cccgctgggg gctggagcac gatgaagaca
     8401 acctgatggc gctggtgtta acgccggaac atctggaatt gcgcaagcgt gatgagccaa
     8461 aacttggcgg catctttgtt gattttgttg gcggagcgat ggcgcaccga cgcaaattcg
     8521 gcggtggtcg cggtgaggcg gtggcgaaag cggtgggcat taaaggcgat tatttgccgg
     8581 atgtggtgga cgccaccgca gggctggggc gcgatgcctt tgtactggct tcagtcggct
     8641 gccgcgtgcg gatgctggag cgtaatccag tggttgccgc gctactcgac gacggcctgg
     8701 cgcgtggtta tgcggatgcg gaaatcggcg gctggttgca ggagcggttg cagttaattc
     8761 acgcctccag cctgacggcg ctaaccgata ttaccccacg cccgcaggtg gtctatctcg
     8821 atccgatgtt cccgcataag cagaaaagcg cgctagtgaa aaaagagatg cgtgtctttc
     8881 agtcgctggt gggaccggat cttgatgccg atggattact ggagcctgca cgcttgctgg
     8941 cgaccaaacg cgtggtggta aagcgcccgg actacgcgcc accgctagcg aatgtcacca
     9001 cgccaaacgc ggttgtgact aaagggcatc gctttgatat ctatgcaggt acgcccgtgt
     9061 aaaagtaaag cagggtagcg ataacgctac cctgctttct gcaagaataa ttaatggctt
     9121 tccggcgtcg caatcatgcg tttcagccac ggcaccatca acagcatcac tactgcaacg
     9181 cccagcgtga ccagaccaat cttaccaaac acgttggtat agacgggcaa cgtctcaagc
     9241 ggatcggtaa tgttgtccgg caccgcggta aatgttgcca catagccgcc cagcaagaac
     9301 gcggcagcct gcgtcaggaa ccacatcccg agaataaagc ccatcaaatg ctgcggcacc
     9361 agggcagcaa tcatcgccag gccaagggcg ctaataaaca gttcacctaa gctctggaat
     9421 aagtacacca gcacgataaa ccatggcgat gtcagccctt gtgcatccgc aaaccacatt
     9481 cccgcagctg ccgccgtcaa aaagcccagt gagcacataa acatgccgag agtaaatttc
     9541 atcggcatcg agaggtcttt gcctttgtta cccagatgcg tgtaaatgcc tgccagtatt
     9601 gggttggcga gtaccaccca gaacgggtta agcgcctgga agctgaccga gttgatggaa
     9661 aaaccgagaa tttcatgatg cacgttgttg atggcaaaga agttcagcga tgttggcatc
     9721 tgggcgtaga gaatgtaaaa caccaccgct tcgagcatca ggacaaaggc gacaaacatt
     9781 ttattgcgcc cggttttatc cagcttgaat gcctgacgaa agaagatgat ggtgacgacg
     9841 atggagagaa caatcagcac cagattggcg acttctacgt tgtgcatcag ccatgcacat
     9901 acgaagatca tcaccacgct gccaagtaat acgtacaaca gtttgctgaa gctcatcggc
     9961 ttgaagtcgg gttcagaacc aatgtctttc accattccac gacaggcgat gtaaaccagt
    10021 aatgcgataa ttaaccccgc cccgcacagg ttgtaggtga ctgaataacc gaatctatca
    10081 gcgatcacag gggccagcga taacgctatc aacgagccga tattgatcga catatagaac
    10141 agggtgaatg cgccatcaag ccgcggatct ttcggcggat agcacttcga aagcaagctg
    10201 gctgggttag ctttaaacag gccgttaccg acagcgatag tccccagggc gatgaaaatc
    10261 aggtcaggct taagtagcga catgccggtc atgaagtagc caatcgccag cacaagtgct
    10321 ccgagaacaa tggtgcgttt ggtccccagc aggtggtcgc cgacatagcc gccaatggaa
    10381 atgaggccat agaccagcgc agcaaaagca ccaaaagtga caaaagcctg ctcttgcgag
    10441 aatccaagct gtttaacgaa gaaaaccgcc agtacgccct gcacgccgta gtagccgaat
    10501 cgctcccata actcgacaaa aaagatcatg aaaaatgggc gaggttgctg cagcatcccc
    10561 atgggtgttg ttgtattcat atttattaac cttccaatac catcctgaaa agtatgaacg
    10621 cgtaagggat aacgcgcgaa aaagtttaca tctgtcagag attcttaata ttgatgcggt
    10681 gaatggatta atacactatt acaacagaaa ataaccagct aatcacacta atgatggaaa
    10741 atatgatggt gttaaatgtt gtattggcag gagattttga ttgcgtacgc agatcgtagg
    10801 ccagataagg ggtttacgct gcatcaggta acaaggcgcg ccaaaaagca aaacgcccgc
    10861 atatgcgggc gttcaggaac acgtcgtgga gggaagatta ttcttcttcg tcgcgcagcg
    10921 gaacaatcag catatcaacg tgaacggtgt tgatcagctg acgtgcggaa gacatcagtt
    10981 tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc catatcgtat ttcttgattg
    11041 catcgaccag aacctggccc aggtcgccgc tgccgctcag ggtttcagtg attgggtagc
    11101 ctgcattagt ggaaagctcg gtcagcgcat gatgtgtctc ttcagagatg cgtttctgca
    11161 tatcacccag attcacatca ataagcccgg tgtataggtc agagtagttt acatctacgt
    11221 ggatcagaga aactttcgca ttgtaggggc gagccataga gactgctttc tctaccagaa
    11281 ctttgctttc cggggagaga tcgaccgcga tgagaatgtg tttataagcc atagtgttac
    11341 tccttccata aagttgtcga tgactggcca gctagcgttt cttgtgccgt tacgtcaccc
    11401 gcgtcctgcg aactacgtgc gcggggctaa cccagccgta aagattcagt gttaagacca
    11461 tccttacctt atagcgaccc ggatgatccg tcaatccgcc ttgcttaccc gttagataaa
    11521 caaattttcg acatgaatat attgatagtg gttaaccttc tggaaaaaaa acaacctgat
    11581 ctcctacact atctatagag ccgctcggat gttggtcacc acaaaagcgg tttctccagg
    11641 acctggtgtt aactgattgg cagcgtatcg ggagcggtag ccccggggtg gaactccgcg
    11701 ggcaggttgc cggggaggag gtatgataag caccgtcgca ttattttggg ctttatgtgt
    11761 cgtttgcatt gttaacatgg cgcgctattt ctcatcacta cgcgcgttgt tagtggtact
    11821 gcgtaactgc gatccattgc tctatcaata tgttgatgga gggggctttt ttacctcaca
    11881 tggccaaccc aacaaacagg tgcgtctcgt ttggtatatc tatgcccaac gttatcgcga
    11941 tcatcacgat gatgagttta ttcgccgctg tgagcgggtg cgtcggcagt ttattctgac
    12001 tagcgcattg tgtggtctgg tggtggtcag cctgattgca ttgatgattt ggcattaagc
    12061 acttcagcaa taataacgca taaaaaaagc tggccagtaa actgacccgc tcgagactga
    12121 ttacaaacgc aaaattgcct gatgcgctac gcttatcagg cctgcataat ctctgcaatc
    12181 tattgaattt gcatgttttt gtaggccgaa taaggcgttc acgccgcatc tggcatgaac
    12241 aaagcgcact atgtcacaat ctgaagcggg ccagctgacc cgctcgtttt ggtgcgtacg
    12301 attacaggaa ctgcaaggag agccagtaca gcccgccgga aagcagtacc gcagccggaa
    12361 gggtaaacac ccaggccatc agaatgctgg tcacggtttt acgctgtaag ccgccaccat
    12421 ctaccaccat cgtccccgcg acagaagagg agagtacgtg agtagtggaa accggcatcc
    12481 cggtataact cgccaggccg atagacactg ccgccgtcat ctgggcagac atcccctgag
    12541 cgtaggtcat gcctttctta ccgattttct caccgatagt cgttgccaca cgacgccagc
    12601 caatcatcgt accgatacct aacgccagcg cgaccgccat gatgatccac accggtgcat
    12661 actcgatggt gctaagcatg tcggacttca gtttcttcaa caggcgctga tcgtcagcac
    12721 tcacgccagg tatcttcact actttgtcga tagtgtcaga aacgcacagc ataatgcggc
    12781 gcatctggct acgttgatca agcgacagct tgtcgtagct ttccacatcg gtagttaaca
    12841 tacctttcag gcggttgagc gcgttaatgg tattcgacgg atggcaatgg aactccgcgg
    12901 gttgcgttgc gccagcttcc ggagccggta ctaactgatc agcaccggta gcctgtttca
    12961 gcagcgcagg atgctgctca aagtaagctt cgacgttgtt gatggcatca cgggtacggg
    13021 tgatttcgta gccagtggca ttcatattca ccacgaagcc tgctggtgcg acaccaatca
    13081 ataccaacat aaccagacca atgcctttct gaccatcgtt cgcgccgtgc gaaaacgcca
    13141 cgccgatagc ggaaaggatc agcgcaatac gcgtccagaa cggcggcttt ttcttgccgt
    13201 ctttcttttc acgctccgct ggggtcaggt ggatacgggc gcgtttcttg gtgccgctcc
    13261 agtagcgacg cagcaagaaa atcagaccgc cagcaaacac caggccgaca ataggggaaa
    13321 caatcagaga accgaaaata cttaatactt tcgggatatt gagtgcatcc accactgacg
    13381 tcccggtcat caacgcattg gttaaaccaa tcccgatgat cgcgccaatc agtgtatgag
    13441 agctggatgc aggtaaacca aagtaccagg tacccaggtt ccagataatc gccgccagca
    13501 acatagagaa caccatggca aggccatgag acgatcccat attaagcagc agatccgtcg
    13561 gcagcatatg cacaatggca taggcaacac tcagaccacc cagcaaaaca cccaaaaagt
    13621 tgaacaccgc cgccataacc acggcgagct gagaacgcat cgcgcgggta tagataacgg
    13681 ttgccacggc gttggctgtg tcatggaaac cattgatggc ttcgtagaac agcacaaaag
    13741 ccagtgcaag caataataac agcccggtat gcaaatccag gccagcaaac aaatgtagca
    13801 taggacgtta cgccattttg aggacatgaa cgcggcgcat tatcagtgac tttcgcggcg
    13861 cgggcaaagt gaaatataga ctttttttga tttgcctcct gtatggattt cactcaaaaa
    13921 ataattatct tatataattc aggcaaatac ttccttttag taatattgat gctggtgtga
    13981 ccactgagga atctttacaa ttcacgcccg ttttttctaa gaggagcgca acgtggaaag
    14041 gtttgatgcc attattatag gcgctggtgc ggcgggtatg ttctgttctg cgctggcagg
    14101 tcaggcagga cgccgggttc tgctgatcga taatggtaaa aaaccagggc gcaaaatcct
    14161 tatgtctggc ggtgggcgct gcaactttac caacctttat gtcgaaccag gcgcttatct
    14221 gagccagaat ccgcattttt gtaagtctgc actcgcgcgt tttacccagt gggatttcat
    14281 tgatctggtc aataaacacg gcatcgcctg gcacgagaaa acgttaggac aactcttctg
    14341 cgatgactcc gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga agggcaatgt
    14401 aaccttcaga ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag gcttcacgct
    14461 tgaactgaac ggcatgactg tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc
    14521 aatgccgggg ctgggcgcat cgccgtttgg ttataagatt gccgaacaat ttggcctcaa
    14581 cgtgctgccg acccgtgcgg gtctggtgcc attcactctg cataaaccgt tgctcgaaga
    14641 gttacaggtg ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa acggcaccgt
    14701 tttccgtgag aacttactct tcacccaccg cggcttatct ggaccggcgg tgttgcagat
    14761 ttccagctac tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg atgtggacct
    14821 cgaaaccttc ctgaatgagc agcgtaacgc acatccgaat cagagcctga aaaacacact
    14881 ggcggttcat ctaccgaagc ggttggttga acgcttacag caactcgggc aaatcccgga
    14941 tgtttcgcta aaacagctca acgtgcgtga ccaacaggca ctgattagca cattgaccga
    15001 ctggcgcgta caacccaacg gcactgaagg ctatcgcact gccgaagtga cgctcggcgg
    15061 cgtggacacc aacgaactct cttcacggac gatggaagcg cgcaaagtgc ctgggctgta
    15121 cttcatcggc gaagtgatgg acgttaccgg ctggctgggg ggctataact tccagtgggc
    15181 gtggtcgagt gcgtgggctt gtgcgcagga tttgattgca gcgaagtcgt cctgacctgt
    15241 tacctaaaga tgaaaattat tagagccgca ttaaaaatga gctaattttg atagtggcta
    15301 tcttgtgatt attttctaat gagcccgtga actgaaaccc tccaggctta atataaggtg
    15361 gaaggaaaga tgattgaaaa ctcacccagt ggccagatct ttataaaata tgaacaggct
    15421 gaaaaagaca gtaactttgt tgttttttat ccttaaccac tatgcattaa tgctgcgtta
    15481 tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt tttatccggt
    15541 acgttgcaat tattttttag cagaacctgc ttctaatatt gaaacgattg agaacaacgt
    15601 aaagcatacc gctcccaaac cgaccaaaac ccgcgaagga ataaaatatc ccatgtcggt
    15661 ctgcgccatt tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg tgaagacggg
    15721 aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cataccagtg ccagtagcca
    15781 gacttttgag aatatgctgt aacagatcat tccgaggtaa ataagaataa tgccgggagc
    15841 cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta ccgtgattga
    15901 gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa acgtattacg
    15961 tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg cggttaaccc
    16021 aagcaatacg tgacccgcga cataatgcgg agttatgtca gcactacgta acagcgtaat
    16081 agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc agttaccgat
    16141 taatgaagaa taagcctgta ccggtgttcc gtcgctcttg ctccccgctg catttttggg
    16201 aatgagcaga aagtgaccgg acgacgctgc caccgtcgat acacatgcgg caatcatacc
    16261 aatgccgaaa ataacatagc cggcgacaaa ctcgtctgcc atcacatcat tgcctgctaa
    16321 cagtgcccaa ccccatatca tggtgataat tgaccctgca tagccaataa tggggaacaa
    16381 tatattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa atgcggtagt
    16441 gaataatgcc aggcatatgg ctgccagaga aatcagcaca tgacccgcga cgaaatactc
    16501 gctttgccct gaactttcca gcacaaagcc acctaacgcg atacaaatta agcccataat
    16561 caaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact cctcaggt
//
LOCUS       DADLTD010000085        16528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLTD010000085 DADLTD010000000
VERSION     DADLTD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.085"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(45..190)
                     /locus_tag="JN701_002165"
                     /pseudo
     CDS             complement(45..190)
                     /locus_tag="JN701_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            complement(299..393)
                     /locus_tag="JN701_002166"
     tRNA            complement(299..393)
                     /locus_tag="JN701_002166"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Sec,seq:tca)
     gene            686..2068
                     /locus_tag="JN701_002167"
     CDS             686..2068
                     /locus_tag="JN701_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HAY7364613.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGELPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2078..4369
                     /gene="yicI"
                     /locus_tag="JN701_002168"
     CDS             2078..4369
                     /gene="yicI"
                     /locus_tag="JN701_002168"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY7364614.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVGETVYGLGERF
                     TALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEKVSK
                     VQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEAT
                     VNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKIC
                     VWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADK
                     LKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTVGEE
                     EAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGGFEN
                     TAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYR
                     EAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEG
                     RWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAFHLF
                     NLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQD
                     GSQAESEQGLVVKPQGNALTITL"
     gene            complement(4422..6131)
                     /locus_tag="JN701_002169"
     CDS             complement(4422..6131)
                     /locus_tag="JN701_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7364615.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6252..7643)
                     /gene="xanP"
                     /locus_tag="JN701_002170"
     CDS             complement(6252..7643)
                     /gene="xanP"
                     /locus_tag="JN701_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7364616.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7923..9128
                     /gene="gltS"
                     /locus_tag="JN701_002171"
     CDS             7923..9128
                     /gene="gltS"
                     /locus_tag="JN701_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY7364617.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9162..11243)
                     /gene="recG"
                     /locus_tag="JN701_002172"
     CDS             complement(9162..11243)
                     /gene="recG"
                     /locus_tag="JN701_002172"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY7364618.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11249..11938)
                     /gene="trmH"
                     /locus_tag="JN701_002173"
     CDS             complement(11249..11938)
                     /gene="trmH"
                     /locus_tag="JN701_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY7364619.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11945..14053)
                     /gene="spoT"
                     /locus_tag="JN701_002174"
     CDS             complement(11945..14053)
                     /gene="spoT"
                     /locus_tag="JN701_002174"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY7364620.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14072..14347)
                     /gene="rpoZ"
                     /locus_tag="JN701_002175"
     CDS             complement(14072..14347)
                     /gene="rpoZ"
                     /locus_tag="JN701_002175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY7364621.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14402..15025)
                     /gene="gmk"
                     /locus_tag="JN701_002176"
     CDS             complement(14402..15025)
                     /gene="gmk"
                     /locus_tag="JN701_002176"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY7364622.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15283..>16528
                     /gene="ligB"
                     /locus_tag="JN701_002177"
     CDS             15283..>16528
                     /gene="ligB"
                     /locus_tag="JN701_002177"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY7364623.1"
                     /translation="MKVWMAILISILCWQSSVWAAWSPARAQEEIFRQQQQIKQWDDD
                     YWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRKMAD
                     KNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISA
                     VPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVF
                     VWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVTDGV
                     VVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLASVM
                     LDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPEN
                     RFNSLTCYFASDVCQEQFISRLV"
BASE COUNT         3754 a         4224 c         4493 g         4057 t
ORIGIN      
        1 agtcgtataa aaaagggtgt tatggagcgt gtaaaacaac atcttcacct taatggcgat
       61 acagttggct gagagccagc agggggcaaa tcacctgcaa ataaacacgg cacatgctgg
      121 cgcgcatatc gcggatgcgt tcgaggacag aagaaccgac aggcggattc ttcagccgct
      181 catcgtccat cacaaaacct tagaccaggt attcctgaag ccgattgaat attgagcagt
      241 agaaaatatc tggattgaca gtattacaag gggttggaga acgtcagaag aggcattttg
      301 gcggaagatc acaggagtcg aacctgtccg ggaccgctgg cggccccaac tggatttgaa
      361 gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca tggaggcggg
      421 gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt tccgcctaat
      481 acacgcagcg tattatcacg aaagcattat agttcatata aaacccatat tttacaatga
      541 catatgagcg gaattaacgc taccacgatc acataaaaca agaatcataa attaataacc
      601 agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc ggtctcagca
      661 cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta aagagaaaat
      721 tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa tgttatatat
      781 gatgttcttt tataccgata tttttggcat tcctgccggt tttgtcggaa ccatgttttt
      841 ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg ccgatcgaac
      901 gcgctctcgc tggggtaaat ttcgtccgtg ggtactgttt ggcgaactgc cattcgggat
      961 cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa tgatctatgc
     1021 agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc cttactgcgc
     1081 attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat cctggcgttt
     1141 tgtgctggcg accgcgggag gcatgctttc tactgttctg atgatgccac tggttaattt
     1201 aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc tttccgtggt
     1261 ggcattcatg atgctggcat tttgtttctt caccactaaa gaacgcgttg aagcaccacc
     1321 tacaacaacg tctatgcggg aagatttacg tgatatctgg caaaacgacc agtggcggat
     1381 tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtactcggtg gggcgatgat
     1441 gtattacgtc acatggattt tgggcacgcc ggaagtgttt gtcgcttttc tcaccactta
     1501 ttgcgtgggt aacctgattg gttccgcact ggcaaaacct ctgaccgact ggaaatgtaa
     1561 agtcactatc ttctggtgga cgaacgccct gctggcagtg attagcctcg cgatgttctt
     1621 tgttcccatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg gtgtgttgca
     1681 tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact acggcgagtg
     1741 gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg tgctcaaact
     1801 ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg gatatgatgc
     1861 ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat tcacgattgt
     1921 tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact cactcacgac
     1981 gcacaatctg aaaaccgtta tggaacagct ggctcagggt aaacgccgtt gccagcaaca
     2041 attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg atggaaactg
     2101 gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg ttgaacagca
     2161 ggataatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgta cctggcagct
     2221 tgatacgcct ttatttacgc tgcgcttttt ctccccacag gaaggtattg tcggtgtgcg
     2281 gattgagcat tttcagggag cgctgaataa cggtcctcat tatccgctca atattttgca
     2341 ggacgtgaag gtcacaatcg aaaacacaga acgttatgct gagtttaaaa gtggcaactt
     2401 aagcgcgcgt gtcagcaaag gtgagttctg gtcactcgat tttctgcgca acggtgaacg
     2461 tattaccggt caggacacga ataatcaacg aaattatatg tttgagcggc ttgatcttgg
     2521 cgttggcgaa acagtttatg gtctgggaga gcgttttact gccctggtgc gcaatggtca
     2581 gacggtagag acctggaacc gggacggcgg cacaagtact gaacaggcgt ataaaaatat
     2641 cccgttctat atgactaacc gtggctatgg ggtgctggtc aatcaccccc agagtgtctc
     2701 ttttgaagtg ggatcggaga aagtctccaa agtgcagttc agcgttgaga gcgaatatct
     2761 cgaatacttt gttatcgacg gtccgacgcc gaaagcggta cttgatcgtt atacccgttt
     2821 tactggtcgt ccggcgctgc cgcccgcatg gtccttcggc ctgtggctaa ccacttcatt
     2881 taccaccaac tacgacgaag cgacggtaaa cagctttatc gatggtatgg cggaacgcaa
     2941 tctgccgctg catgttttcc actttgactg tttctggatg aaagccttcc agtggtgcga
     3001 ttttgagtgg gacccgctga ctttcccgga cccggaaggg atgatccgcc gcctgaaagc
     3061 gaaagggctg aaaatctgcg tctggattaa cccctatatc ggtcaaaaat cccccgtctt
     3121 taaagagtta caagagaaag gctatttact caaacgcccg gacggttcgc tatggcagtg
     3181 ggataaatgg cagccaggtc tggcgattta tgactttacc aatccggatg cctgcaaatg
     3241 gtacgccgac aaactgaaag gtctggtcgc gatgggcgtt gattgcttta agaccgactt
     3301 tggcgaacgt atcccaactg atgttcagtg gtttgacggt tccgatccgc agaaaatgca
     3361 taaccattat gcgcacatct acaacgaact ggtgtggaac gtgctcaagg acaccgttgg
     3421 tgaggaagaa gctgtcttgt ttgcccgctc ggcctccgtc ggtgcgcaga aattcccggt
     3481 acactggggt ggcgattgtt acgctaacta cgaatcaatg gcggaaagcc tgcgcggtgg
     3541 tttgtctatt ggcctttcag gttttggctt ctggagccac tatatcggcg gctttgaaaa
     3601 taccgctccg gcgcacgttt acaaatgctg gtgcgcgttt ggtttgctct ccagccatag
     3661 ccgtttacac ggtagcaaat cttatcgtgt gccgtgggcc tacgatgatg agtcctgtga
     3721 tgtggtgcgc ttcttcacgc aactgaaatg ccgcatgatg ccgtatctgt atcgtgaagc
     3781 tgcgcgtgca aacgcgcggg gtacgccgat gatgcgggcc atgatgatgg agttcccgga
     3841 cgatccggct tgtgattacc ttgaccgtca atacatgtta ggcgacaacg tgatggttgc
     3901 gccggtgttc actgaagcgg gcgatgtgca gttctacctg ccggaaggtc gctggacaca
     3961 cctgtggcac aacgatgagc tcgacggtcg ttgctggcat aaacagcagc acagcttcct
     4021 gagtctgccc atttatgtgc gtgataacac cctactggcg ctgggcaaca acgatcaacg
     4081 tcccgattac gcgtggcacg aaggcacggc attccacctc tttaatctgc aagacgggca
     4141 tgaagccgtc tgtgaagtgc ccgctgctga cggatcggtg atctttactt taaaagcagc
     4201 acgtactggc aacacgatta ctgtgactgg tgcgggcgag gcgaagaact ggacactgtg
     4261 cctgcgcaat gttgtgaaag taaatggtct gcaagacggt tcgcaggctg aaagtgagca
     4321 ggggctggtg gtgaagcctc aagggaatgc gctgacaatt acgttgtaat ctattgatgg
     4381 aatgccgggt acgtctgagt cgtacccggc gagattgatg tttactgcac cagctctgcc
     4441 gacgtaactt caccgctgga aacgatgcca gcattcatgg tttgagtcac ttgctgcttt
     4501 gcggcgttca cggcatgcaa ttgcccgcta accgtaggtt tcagcggggc attggcctga
     4561 atatctccac tggcggtaag ctgaatattg ccgtcgccag tcaacggtaa ttcaggccat
     4621 ccccattgtt gcaaaatatt caccggcaca ccgcgtccat tcaggctgat atgggtctga
     4681 cgttgtggcg tttgtgaaac actggcagtg gcttccagaa tgcctttttc agtaaatgca
     4741 ctcagttcgc tgatattcac cgtgctgctg ttggcggtca gcgccagcga cgggcgacga
     4801 acatcaacac gattgaatgt cgcggcggcg gcattcagat tcgccgagcc actccagacg
     4861 ccccatttat gatcggtaac cagcgtcagg ttggcaccgt aaccatcgag cgtggtgagc
     4921 tgccacggga agtcagggtc gatatcaatg atcagattgc ggctggcgct aaatctcttc
     4981 agttgcaggc tgtttaacca accgggtgtc gtttccatcc acaactgttg ccagtttttc
     5041 ggcaaggtat attccagccc ggcaattgcc gcatcatcaa ggatcaacgt tttcccgtca
     5101 cgcagccagt tccctgacgt tctgaccata cccccttccc agcggctggt gaactggcgc
     5161 agcgctacgc cctgcgggga aaattccgcg tttataatcg ggtcaaataa atgcagcgaa
     5221 ccataaatga actcgctagc gttcatcgac agtttgcctt cttgtgtctg ccagtcatct
     5281 ttactgaagg tcatgttgcg caagctgaga tcgaggtcgg tcaccgccca gtccggacct
     5341 tgcaaatgag catcgatcac ttccaggcga ccaatctgca acgacgggac agagcgtaat
     5401 ggcgcaaaga agtcggttag cgatttttcg ctttgtagac gtatatccgc catgcgcagg
     5461 ttttccactt gccagctgcc gtcggcgtta cgctgcgcgt ttccggttaa tgtcccgcgg
     5521 gcgatgtcgg cacccaggtt agtcagcgta acgcgatcgt tatcaatact gccttcaatc
     5581 agtacattgg tggcaggaac atcattgagc gaaagcgatc cggcactaaa ctgaatctgc
     5641 gccttcgtac ccagcacttt accggctttt ggtgaccacg gaactacgcc gccatttacc
     5701 cgctgcgcgc tcagtttcca ttcgctattc gggctattaa acgccatatc acgcagttgc
     5761 agacgatcgg ctttgaacgg tagcggcgcg gtctggtcgg tgagattcag cgtcccgttt
     5821 tccagcagga tggtatcgac atggcgtggt tcggtcagtt gccgactgct tagcgcaatg
     5881 tcgacacttt tggccaccag ggtcgcgggc tgaccatcac gaccaaacgt gacattctcc
     5941 agcacgatat gagatggcgc ggaaaaacgg tgatccatcg ccccgaaggc cagatgatag
     6001 tcgctattct cggaaaccca tgcgctgata tgttctgctc cccagcgggt ttgcagaaga
     6061 aaatagaggc cagcgatcac cactaacaga gcgatgagaa tgtagagaag cagcttccca
     6121 ataaatttca tggtcttcca ttccgtgaaa tgcacataag agagttatgc acgatttacg
     6181 cgcaatcctc aaggcgggaa tggtgaaaga ggtgagactg cggcggtaat tatcttaccg
     6241 ccgccagtga attactgttt ttctggtggg aaaatcagat tcagcacgat ggcagtaata
     6301 ccgcccgcgg cgatcccgga ggagagcagg tttttcagcc attcaggggc aaactgcaaa
     6361 atcagcggct gctgagacac gcccagacca accgccagcg acagcgcgat aatcagaatc
     6421 gcccgacggt tcagcggctc acgagaaacg atacgcacac cggaggcggc gatggtgcca
     6481 aacattacaa gcgttgcgcc gcccagaacc ggttctggaa tgtgttgtac aaaaccgctc
     6541 actgccggga acagacccag cacgatcaac atcagcgcga cgacaaaacc gacatagcgg
     6601 ctggcaacac cagtcaactg gatcactccg ttgttttgcc cgaagcagga gttcgggaag
     6661 gtgttaaaca ccgccgaaac aaacgagttc aggccgtttg ccagcacgcc gcctttcagg
     6721 cgtttcatgt acagcggacc ggacactggc tgttcggaaa cgtcagaggt cgacgtgata
     6781 tcgccaatgg tttccagcga agtgatcata aagaccagca tcagcggcag cagcagactc
     6841 cattcaatgc caagaccgta atagagcggc gttggcacca taatcagttc ttgcgtcatc
     6901 ggttcgttgc tttctggcaa catgcccata aaccacgcca gcgcatatcc ggccgccatc
     6961 gcaatcacca gtgaggccac gcgtaagtaa gggttacgtt gacggttaag caggataatt
     7021 aaggctaaga ccacgcctgc cagcagcaga tttttcggtg cgccgaaggt gttatcgctc
     7081 atggctgcgt aaccgccgcc aatggacgtt aacccaacct gaattagcga caggccgata
     7141 atcatcacca caacgccaga aaccagcggc gtaataatgc ggcgcgccag atgcagaacg
     7201 cgggagatca ccatctcggt gcaacttgcc agcatcaacg tgccgaacaa agccgccatc
     7261 atggtaggaa catcagcacc accggttttc agcgcggtac cgcccataat cagcggggca
     7321 acaaagttga agctggtgcc ctgaatagac aacagcccgg agccaaccgg accccaggtc
     7381 ttaatttgaa taatcgatgc cacaccggag gcaaacagcg acatactaat aatgtgttgc
     7441 gtgtcttgtg ccggtaaacc cagcgcctgg cagattaata gcgctggcgt gatcaccgca
     7501 acgaacatcg ccagcagatg ctgacaggcg gcaaacaggg tttgaggaag cggcggacga
     7561 tcttcaagac ggtaaatcag ttcgctgttt tgagtctgcg caaccggttg cgcattttct
     7621 gactcgaggg tggaaacaga catcgaaaac aatcccgtgg tagcaaagcg ggcattttag
     7681 ctgaccgaaa gagaaaagca aacgtttgcc acatcctcat ctaaatattt aaatgagata
     7741 aatgcacttt tttatataac ttttggtatt tttctgctta aaatccatgc catttgttca
     7801 ttaacagctg agaatttgac ccaggaaagc ataactcgat acccgtcata gttcatgaag
     7861 ttgccactgc gcgatgaagt atgacgagta tgaaagagtg atgcggatac aaaggagtaa
     7921 ctatgtttca tctcgatact ttagcaacgc ttgttgccgc aacgctgacg ttgctgctcg
     7981 ggcgtaagtt ggtccattcc gtctcctttt tgaagaaata caccataccg gaacctgttg
     8041 cgggtggttt gttggtggcg ctggcgctac tagtactgaa aaaaagcatg ggctgggaag
     8101 tcaactttga tatgtccctg cgcgatccgt taatgctggc tttcttcgcc accattggcc
     8161 tgaacgccaa cattgccagt ttgcgtgccg gtgggcgtgt ggttggcatc ttcttgattg
     8221 tggttgttgg tctgttggtg atgcaaaatg ccattggcat tggtatggct agcctgttag
     8281 ggcttgatcc gctgatgggg ctgttggccg gttctattac gctttccggc ggtcacggta
     8341 cgggcgctgc gtggagtaaa ttgttcattg aacgttatgg cttcaccaat gcgacggaag
     8401 tggcgatggc ctgtgcaacg ttcggtctgg tgctgggcgg cttgattggc ggtccggtgg
     8461 cgcgctatct ggtgaaacac tccaccacgc cgaacggtat tccggatgac caggaagtcc
     8521 cgacggcgtt tgaaaagccg gatgtgggac gcatgatcac ctcgttggtg ctgattgaaa
     8581 ctatcgcgct gattgctatc tgcctgacgg tggggaaaat tgttgcgcaa cttttggctg
     8641 gcactgcttt tgaactgccg accttcgtct gtgtactgtt tgttggcgtg attctgagca
     8701 acggtctgtc aataatgggc ttttaccgcg tctttgagcg tgcggtatcc gtgctgggta
     8761 acgtaagctt gtcgttgttc ctggcgatgg cgttgatggg gctgaaactg tgggagctgg
     8821 cttcgctggc gctgccgatg ctggcgattc tggtggtaca gaccatcttc atggcgttgt
     8881 atgccatctt cgttacctgg cgcatgatgg gcaaaaacta cgatgcggca gtgctggctg
     8941 cgggtcactg tggttttggc ctcggtgcaa cgccaacggc aatcgccaac atgcaggcga
     9001 tcactgaacg ctttggcccg tcgcacatgg cgtttttggt ggtgccgatg gtcggtgcgt
     9061 tctttatcga tatcgtcaat gcgctggtaa ttaagttgta tttgatgttg ccgatttttg
     9121 ccggttaacc gatgaagcgg cggtcttctc accgccgcct tttacgcatt cgagtaacgt
     9181 tctgtctccg gcatccagcg ttctatcaag gcttttgcct gttgtgggta acgttcgtga
     9241 atatgtcgcg ccaggcgctg aacttccggg atcatcgcct gatcgcgcag taaatccgcc
     9301 actttaaatt cagcattacc cgtctgacgc gtgcctaaca attcgccagg gccgcgaatc
     9361 tccagatctt tttgcgcaat cacaaaaccg tcgttactgt cgcgcagcac ttgcaggcga
     9421 atttgcgccg ttttagaaag cggcgttttg tagagcagca cgcagtgaga agccaccgcg
     9481 ccacgaccta cgcgcccgcg cagctggtgt aactgcgcca gacccagacg ctcagggttt
     9541 tcgataatca tcaggctggc gttaggcaca tcaacaccga cttcaataac ggtagtggca
     9601 accagcagat gtagctcacc ttgtttaaac gacgccatca ccgcctgttt ctcggctggt
     9661 ttcatccgcc cgtgtaccag gccaacattc aattctggta gcgccagttt caactcttcc
     9721 caggtagctt ccgccgcctg cgcttccagt aactctgact cttcaatcaa cgtacaaacc
     9781 cagtacgcct gacgaccttc agttatgcag gcatggcgca cgcggtcaat gatgtcggta
     9841 cggcgggtat caggaatagc gaccgtagtc actggcgtgc ggcctggcgg cagctcatct
     9901 atcaccgagg tatcgagatc ggcatacgcc gtcattgcca gcgtgcgggg gatcggcgtg
     9961 gcggtcatga tcaactgatg cggatggaag ccttgctgct ggcctttctc ccataacgcc
    10021 agacgctgat gcacgccaaa gcgatgttgt tcgtcgataa tcaccagcgc cagaccgtta
    10081 aactgcacct gttcctggaa gatggcgtgc gtaccaacaa tcatctgcac ctgaccgctg
    10141 gcgatggctt cctgctgaga cagtcgcgct ttgcctttct gcttaccggc gagccagccc
    10201 acttcgatac cgagcggttc aaaccagttg cggaagttat tggcgtgctg ttcggcgagt
    10261 aattcggttg gtgccatcaa tgccacctgt ttgccgtggg caatcgcacg cagcgcggcg
    10321 agggcggcga ccagcgtttt accggaacct acatcgccct gtaccaggcg catcatcggc
    10381 acatccagcg ccatatcgcg ctcgatctcc gccactacgc gtgcctgtgc gcccgttggc
    10441 ttgaacggta aggctgcgag gagtttattt ttcagcgcat cattggcgct cagcggctgg
    10501 gcatgaaaac gctgtgctcc ggcacgtaag gctaacatgc tgaggttgtg cgccagcagt
    10561 tcttccagaa tcagacgacg ttgcgccgga tgctgcccgg tttccagatc gctaagctgt
    10621 agcgtcggtg gcgggcggtg caaagtgcgc aacgcttccg gtagcgtcat cattccttgt
    10681 gacagttccg gcggcaggag ttcttcaatg gcgcaggtgt cgagcagatc cagcgcctgg
    10741 tcggttaatt tacgcagcgt ggcctgcttt acgccttccg ttgttggata aaccggcgtg
    10801 agcgtttcct gtaattctgg cgtgctgaga tcgccctgca cgcggtattc cgggtggatc
    10861 atctccgcac catatttacc gcgctttgct tcgccataag ccagtacacg gcggcccgtc
    10921 gccaggctat ttttcattgc cgcgctgaaa ttgaaaaagc gcatggtgag gatgccggaa
    10981 ccgtcgctga tctggcaggt catcatccgc cgaccgccga aggagatatt gcagttcagc
    11041 acttcgcctt ccaccgtggc ataaacgccc ggcagtagtt ctccgatggg gtagagatgg
    11101 gtgcgatctt cgtagcgcag gggaaggtgt aagagtagat cctgcacggt atgcaggttg
    11161 attttcgcca gtttgttact aagtgctgcg ccaacgcccg ttagggaact gagtgggaca
    11221 gcatctaaca ggcgacctgt catggcactt accctgcagc ctgcatagta gcccaccagt
    11281 cggcatcagc ttcgatctcg ccttgctgat tgacgtgggg ataaggcagg cctttgcgtt
    11341 ttgcgacttt cgccagcacc ggatagccgc cttcaaacaa caggcgttgt tgctctgctt
    11401 ccggcaacat gctgttttca cgcaggtaca tgcctgcatt ttgccgctga cgctgggctt
    11461 cgtaaagaat gagggctgag gcaacggaaa cattcagcga ctgcaccatg ccgatcatcg
    11521 gaatgatgat gtcctgatcc gccagggcca atgcttcctg cgtgatgccc gttttctcct
    11581 gtcccatcaa aatgcaggtc gggcgagtgt aatcaatttc gcggaaatcg acagcgttat
    11641 cagaaagatg ggttgccaga atctgcatgc cctggccttt gagatgagcg acggcatcgc
    11701 caatggtgcg gtgtgttttc acctgtaccc agctgttact acccgccgct gccgaagcca
    11761 tggtgcgcat gcggctacca ggccagacgg cgtgaacttc atgtacgcca acggcatctg
    11821 cggtacgaat aatcgcagaa acgttatgag gtttgtggac ctgctccatg cagacggtca
    11881 gatcaggctg ccgcctggcg agcatttcgc agatgcgtgc ataacgtgtt gggttcataa
    11941 aacattaatt tcggtttcgg gtgactttaa tcacgtctgg catcacgcgg attttgcgca
    12001 tgatattcgc cagatgcaca cggtcacgag cggtcagacg aataaaggcg ctgtagacgc
    12061 gaccatcttt ctcttccgta ttcaaacttt gaatattcga agtcgtggtg ttaattgccg
    12121 ccgtcaggtt tgccagcgca ccctgatgat tgaacatctc caccttgatt tcggtgatga
    12181 actcctgcgc cgtctcttta tcccattcca cagccataaa cttctctggc tctttctggt
    12241 agccacggat gttacggcag gattcatggt ggatcaccag acctttaccg gggctgacgt
    12301 gggcgataat cgggtcgcca ggaatagggc ggcagcattt cgcaaaggtg atcagcacgc
    12361 catccgcgcc tttgatcggc agatgcccgt ggctttgagt cgccggtgga atggaggcgt
    12421 ccccatgttg tagatttttc gcgaccacca cgctcattgc gttaccaagg ccgatttctg
    12481 ccagcagatc gtcaagcgtt gccagcttca tgcgatccag ctcgcgctga atattttcct
    12541 gcgggatttc attgagctta cggctaccac ccaaagcatg gttgagcaga cgacggccca
    12601 ggcttacaga atcatcacgc ttgaggtttt tcagcaactg acgaattttg gcgcgcgctt
    12661 tcgagctaac gacaaagttc agccaagcgg cattcgggcg agcgcccgga gcggtaatga
    12721 tttcaacggt ctgaccgcta gtaagcggct gcgacagcgg gtaaggctgg cggtcgacgc
    12781 gtgcgcccac gcaggcatgg ccgatatcgg tatgcactgc ataagcgaag tcgacgggcg
    12841 ttgcaccggc aggcagctcg acaatgcgcc cttccggtgt gaaaacgtaa atctcatccg
    12901 ggaagagatc ggatttaacg ctctcgataa attcaaacga actaccggcg ctctgttgca
    12961 gctccagcag gctttgcatc cagcgctggg cgcgtatttg tgcggtagta ctggtttcgc
    13021 cgtgctcttt ataagcccag tgcgcggcaa cacccatctc cgccatctga tccatatctt
    13081 cggtacggat ctggacctca accggcacgc cgtgcgggcc aatcatcgag gtgtgcaacg
    13141 actgatagcc gttcgctttt ggaatggcga tatagtcttt cacgcggccc ggacgcggct
    13201 tgtacaggct gtgcatctgg cccagcacgc gataacaggt gtcagaatca ttgacgatca
    13261 cgcggaaagc gtagatgtcc atgattgagt gaaaacgctg ctctttgagc accattttgc
    13321 agtaaatcga ataaagatgc ttttcgcgac cactgacgcg gcacggtatt cccgcttcct
    13381 gcaaacgccc ttcgatttca gaaagaatct tctggatcat ctctttacgg ttgccgcgcg
    13441 cggctttcac cacttctttg attacgcgat aacggttggg atacagcgcc tcaaaaccca
    13501 gctcttcgag ttcggtttta atgtggtgga tacctaaacg gtgcgccagc gggctgtaaa
    13561 tttcgagagt ttcacgggcg atgcggcgac gtttgtccgg gcgaagtgag cccagcgtgc
    13621 gcatgttgtg ggtacggtcg gcaagtttga tgaggatgac gcggatatcc tgcaccatcg
    13681 ccataatcat cttgcgaaag ttttcggcct gcgcctcttt cttatcgcgg aacttgagtt
    13741 tatcaagttt cgacaccccc tctaccagct cggcgacgct tttaccaaaa agctgttcca
    13801 tatcctggta ggtggcggga gtatcttcaa tcacgtcatg cagcagcgcc gccatcagcg
    13861 tttcatagtc gagtttcatc tcggccagaa tgcaggcaac cgctaccggg tgcgtgatat
    13921 agggttcacc gcttgaacgt gtttgcccct cgtgagcatc acgtgcaacg agatacgcct
    13981 gccgcagacg cttgatttgg tcttccggca ggtaggtttg aatcagttga ttcaggcttt
    14041 caaacagata caagggcgac ccgctttgtg attaacgacg accttcagca atagcggtaa
    14101 cggcttgtaa ttcagcggct tcctgctctt gctgttcctg gcgttcgcga acgtcgagga
    14161 tctggttgtt gatcagacct tcttcgattt cgcgcagcgc gattacagtg gttttatcgt
    14221 tttcttccgg taccagcgga tcctttccgc ctacctgcat ctgacgagcg cgacgcgcgg
    14281 cgaccagtac caggtcaaaa cggttaccaa ttttctctac agcgtcctga acagttacgc
    14341 gtgccatact taaaaagctc cacaggtgaa gaaatgactg ggcatgatac tgaaatcagg
    14401 ttcagtctgc caacaatttg ctgattaaag cgtcatgacg ctgcttttgg cggctcatgc
    14461 gcagacgttc ggcgcgaata atggtcttca aatcggtcaa cgcggtatcg aagtcatcat
    14521 tcacaatcag gtaatcatat tcgtcgtaat ggctcatttc tgcaacagct tgcgccatac
    14581 gctttgcaat gacctcttcg ctgtcctgac cgcgaccgcg tagacggcgg tccagttcaa
    14641 ttttagacgg cggtaaaata aagatactcc gcgcgtgcgg catcttctgg cgaatttgct
    14701 gcgcgccctg ccagtcgata tcgagaaaaa catcgacacc ggtcgccagt acttgctcaa
    14761 tggcctcacg cgaagtgcca tagtaattac caaaaacttc tgcgtgttcg aggaacgcat
    14821 ctctgctaat catttcttta aattcatcat gattaacaaa gaaataatgt tcaccgtgga
    14881 cttcaccagg acgcggttgg cgtgtggtgt gtgaaacaga aacctgggtg tcatacaacg
    14941 gttgggtttt taataaagcc tgaatcaggc tggatttacc cgcgccactg ggggcagaaa
    15001 caatataaag cgtgccttga gccatgagta tctttcgtat gtgattagcg aaataaagcc
    15061 tacatacgag cttattatac acggcgcggc catgtgacgt agcccttgct cacacttttt
    15121 gcgcaaagtt gttgcgtttt gcgtagctgc ttccgcattt gcctgtgatt tactgcaata
    15181 aagacgattc tcaggaagcg tcctcgcaga ctccgttttt gccactcggc tttgccttca
    15241 ccacgttgta tatctgccag cctgactaca gcggaggtga agatgaaagt atggatggcg
    15301 atattaataa gtatcttgtg ctggcaatca tctgtgtggg cggcctggtc gccagccaga
    15361 gcacaggaag aaattttccg ccagcaacag caaataaaac agtgggacga tgactactgg
    15421 aaggaaggaa aaagtgaggt ggaagacggt gtttacgatc agttaagcgc ccgtcttacg
    15481 cagtggcaac gctgctttgt gagcgagccc cgcgacgtga tgatgccgcc tttaaatggt
    15541 gcggttatgc atccggttgc ccatacgggt gtacgcaaaa tggcggataa aaacgcatta
    15601 agtctgtgga tgcgagaacg tagcgatctt tgggtgcagc caaaagttga tggcgttgcg
    15661 gtaaccctgg tttatcggga cgggaaactg aacaaagcaa tcagtcgcgg taatggcctg
    15721 aaaggcgagg actggacgca gaaagttagc ttaatttccg ctgtgccgca aaccgttagc
    15781 gggcctttag ccaacagtac gcttcagggg gaaatatttc tccagcgcga ggggcatatc
    15841 caacaacaaa tggggggaat aaatgcccgc gcaaaagttg ctggcttgat gatgcgccag
    15901 gacgatagcg acacgctgaa ttctctgggc gtttttgtct gggcatggcc ggatggaccg
    15961 cagttaatgt ctgatcgttt aaaagagctg gctaccgcag gttttactct aacgcagacg
    16021 tatacccgtg cggtgaaaaa tgctgatgaa gttgcgcgcg tacgcaatga gtggtggaaa
    16081 gcggaattac ccttcgtcac cgatggcgta gttgtacgag cggcgaaaga gccagaatcc
    16141 cgccattggc taccgggcca ggcagagtgg ctggtggcct ggaaatatca acctgtagct
    16201 caggttgccg aagtgaaggc gattcagttt gcggtgggta agagcggtaa aatatcggtg
    16261 gttgcgtcac tcgcatctgt catgctggat gataaaaaag tccagcgggt gaatattggt
    16321 tccgtcaggc gctggcagga gtgggatatt gcgcctggtg atcagattct cgtcagcctt
    16381 gccggtcagg gtattcctcg cattgatgat gtggtgtggc gcggtgcaga acgtacaaaa
    16441 ccgacaccgc cagaaaaccg ctttaactcg ttgacctgct actttgcttc tgatgtttgt
    16501 caggaacagt tcatttcacg cttagtct
//
LOCUS       DADLTD010000086        16472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLTD010000086 DADLTD010000000
VERSION     DADLTD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.086"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(118..1401)
                     /gene="gltA"
                     /locus_tag="JN701_002178"
     CDS             complement(118..1401)
                     /gene="gltA"
                     /locus_tag="JN701_002178"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY7364624.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1607..1714)
                     /locus_tag="JN701_002179"
     CDS             complement(1607..1714)
                     /locus_tag="JN701_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364625.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2095..2499
                     /gene="sdhC"
                     /locus_tag="JN701_002180"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2095..2499
                     /gene="sdhC"
                     /locus_tag="JN701_002180"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7364626.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2493..2840
                     /gene="sdhD"
                     /locus_tag="JN701_002181"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2493..2840
                     /gene="sdhD"
                     /locus_tag="JN701_002181"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY7364627.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2840..4606
                     /gene="sdhA"
                     /locus_tag="JN701_002182"
     CDS             2840..4606
                     /gene="sdhA"
                     /locus_tag="JN701_002182"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY7364628.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4622..5338
                     /locus_tag="JN701_002183"
     CDS             4622..5338
                     /locus_tag="JN701_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY7364629.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5639..8440
                     /gene="sucA"
                     /locus_tag="JN701_002184"
     CDS             5639..8440
                     /gene="sucA"
                     /locus_tag="JN701_002184"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY7364630.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8455..9672
                     /gene="odhB"
                     /locus_tag="JN701_002185"
     CDS             8455..9672
                     /gene="odhB"
                     /locus_tag="JN701_002185"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY7364631.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9948..11114
                     /gene="sucC"
                     /locus_tag="JN701_002186"
     CDS             9948..11114
                     /gene="sucC"
                     /locus_tag="JN701_002186"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY7364632.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11114..11983
                     /gene="sucD"
                     /locus_tag="JN701_002187"
     CDS             11114..11983
                     /gene="sucD"
                     /locus_tag="JN701_002187"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY7364633.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12087..12809)
                     /gene="mngR"
                     /locus_tag="JN701_002188"
     CDS             complement(12087..12809)
                     /gene="mngR"
                     /locus_tag="JN701_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY7364634.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12918..14894
                     /gene="mngA"
                     /locus_tag="JN701_002189"
     CDS             12918..14894
                     /gene="mngA"
                     /locus_tag="JN701_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY7364635.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALRLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWEGVDGPEAVDLVVLLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQALTLKRSDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALALGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMV
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMP"
     gene            14912..>16472
                     /locus_tag="JN701_002190"
     CDS             14912..>16472
                     /locus_tag="JN701_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY7364636.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVL"
BASE COUNT         3951 a         4134 c         4489 g         3898 t
ORIGIN      
        1 aatatatgaa tgtccgatag tggattttat ttttacaatt ttttgtgttt aggcatataa
       61 aaattaaccc gccatttgaa cggcgggtta aaatatttac aacttagcaa tcaaccatta
      121 acgcttgata tcgcttttaa agtcgcgttt ttcatatcct gtatacagct gacgcggacg
      181 ggcaatcttc ataccgtcac tgtgcatttc gctccagtgg gcgatccagc caacggtacg
      241 tgccattgcg aaaatgacgg tgaacatgga agacggaata cccatcgctt tcaggatgat
      301 accagagtag aaatcgacgt tcgggtacag tttcttctcg ataaagtacg ggtcgttcag
      361 cgcgatgttt tccagctcca tagccacttc cagcaggtca tctttggtgc ccagctcttt
      421 cagaacttca tggcaggttt cacgcattac ggtggcgcgc gggtcgtaat ttttgtacac
      481 gcggtgaccg aagcccatca ggcggaaaga atcattttta tctttcgcac gacgaacaaa
      541 ttccggaatg tgtttaacgg agctaatttc ttccagcatt ttcagcgccg cttcgttagc
      601 accaccgtgc gcaggtcccc acagtgaagc aatacctgct gcgatacagg caaacgggtt
      661 cgcacccgaa gagccagcgg tacgcacggt ggaggtagag gcgttctgtt catggtcagc
      721 gtgcaggatc agaatacggt ccatagcacg ttccagaatc ggattaactt catacggttc
      781 gcacggcgtg gagaacatca tattcaggaa gttaccggca taggagagat cgttgcgcgg
      841 ataaacaaat ggctgaccaa tggaatactt gtaacacatc gcggccatgg tcggcatttt
      901 cgacagcagg cggaacgcgg caatttcacg atgacgagga ttgttaacat ccagcgagtc
      961 gtgatagaac gccgccagcg cgccggtaat accacacatg actgccattg gatgtgagtc
     1021 gcgacggaaa gcgtggaaca gacgggtaat ctgctcgtgg atcatggtat gacgggtcac
     1081 cgtagtttta aattcgtcat actgttcctg agtcggtttt tcaccattca gcaggatgta
     1141 acaaacttcc aggtagttag aatcggtcgc cagctgatcg atcgggaaac cgcggtgcag
     1201 caaaatacct tcatcaccat caataaaagt aattttagat tcgcaggatg cggttgaagt
     1261 gaagcctggg tcaaaggtga acacaccttt tgaaccgaga gtacggatat caataacatc
     1321 ttgacccagc gtgcctttca gcacatccag ttcaacagct gtgtccccgt tgagggtgag
     1381 ttttgctttt gtatcagcca tttaaggtct ccttagcgcc ttattgcgta agactgccgg
     1441 aacttaaatt tgccttcgca catcaacctg gctttacccg tttgttattt ggctcgccgc
     1501 tctgtgaaag aggggaaaac ctgggtacag agctctgggc gcttgcaggt aaaggatcca
     1561 ttgatgacga ataaatggcg aatcaagtac ttagcaatcc gaattattaa acttgtctac
     1621 cactaataac tgtcccgaat gaattggtca atacttccac actgttacat aagttaatct
     1681 taggtgaaat accgacttca taacttttac gcattatatg cttttcctgg taatgtttgt
     1741 aacaactttg ttgagtgatt gtcaaattag atgattaaaa attaaataaa tgttgttgtc
     1801 gtgacctgga tcactgttca ggataaaacc cgacaaacta tatgtaggtt aattgtaatg
     1861 attttgtgaa cagcctatac tgccgccagg tctccggaac accctgcaat cccgagccac
     1921 ccagcgttgt aacgtgtcgt tttcgcatct ggaagcagtg ttttgcatga cgcgcagtta
     1981 tagaaaggac gctgtctgac ccgcaagcag accggaggaa ggaaatcccg acgtctccag
     2041 gtaacagaaa gttaacctct gtgcccgtag tccccaggga ataataagaa cagcatgtgg
     2101 gcgttattca tgataagaaa tgtgaaaaaa caaagacctg ttaatctgga cctacagacc
     2161 atccggttcc ccatcacggc gatagcgtcc attctccatc gcgtttccgg tgtgatcacc
     2221 tttgttgcag tgggcatcct gctgtggctt ctgggtacca gcctctcttc ccctgaaggt
     2281 ttcgagcaag cttccgcgat tatgggcagc ttcttcgtca aatttatcat gtggggcatc
     2341 cttaccgctc tggcgtatca cgtcgtcgta ggtattcgcc acatgatgat ggattttggc
     2401 tatctggaag aaacattcga agcgggtaaa cgctccgcca aaatctcctt tgttattact
     2461 gtcgtgcttt cacttctcgc aggagtcctc gtatggtaag caacgcctcc gcattaggac
     2521 gcaatggcgt acatgatttc atcctcgttc gcgctaccgc tatcgtcctg acgctctaca
     2581 tcatttatat ggtcggtttt ttcgccacca gtggcgaact gacatatgaa gtctggatcg
     2641 gtttcttcgc ctctgcgttc accaaagtgt tcaccctgct ggcgctgttt tctatcttga
     2701 ttcatgcctg gatcggcatg tggcaggtgt tgaccgacta cgttaaaccg ctggcattgc
     2761 gcctgatgct gcaactggtg attgtcgttg cactggtggt ttacgtgatt tatggattcg
     2821 ttgtggtgtg gggtgtgtga tgaaattgcc agtcagagaa tttgatgcag ttgtgattgg
     2881 tgccggtggc gcaggtatgc gcgcggcgct gcaaatttcc cagagcggcc agacctgtgc
     2941 gctgctctct aaagtcttcc cgacccgttc ccataccgtt tctgcgcaag gcggcattac
     3001 cgttgcgctg ggtaataccc atgaagataa ctgggaatgg catatgtacg acaccgtaaa
     3061 agggtcggac tatatcggtg accaggacgc gattgaatat atgtgtaaaa ccgggccgga
     3121 agcgattctg gaactcgaac acatgggcct gccgttctcg cgtcttgatg atggtcgtat
     3181 ctatcaacgt ccgtttggcg gtcagtcgaa aaacttcggc ggcgagcagg cggcacgcac
     3241 tgcggcggcg gctgaccgta ccggtcacgc actgttgcac acgctttatc agcagaacct
     3301 gaaaaaccac accaccattt tctccgagtg gtatgcgctg gatctggtga agaaccagga
     3361 tggcgcggtg gtgggttgta ctgcactgtg catcgaaact ggtgaagtgg tttatttcaa
     3421 agcccgcgct accgtgctgg cgactggcgg ggcagggcgt atttatcagt ccaccactaa
     3481 cgcccacatt aacactggcg acggcgtcgg catggctatc cgcgcaggcg taccggtgca
     3541 ggatatggaa atgtggcagt tccacccgac cggtattgcc ggtgcgggcg tactggtcac
     3601 cgaaggttgc cgtggtgaag gcggttatct gctgaacaaa catggcgaac gctttatgga
     3661 gcgttatgcg ccgaacgcca aagacctggc gggccgtgat gtggtggcgc gttccatcat
     3721 gatcgaaatc cgtgaaggtc gcggctgtga tggtccgtgg gggccacacg cgaaactgaa
     3781 actcgaccac ctgggtaaag aagttctcga atcccgtctg ccgggcatcc tggaactctc
     3841 ccgtaccttc gctcacgtcg atccggtgaa agagccgatc ccggttatcc caacctgtca
     3901 ttacatgatg ggcggtattc cgaccaaagt gaccggtcag gcgctgactg tgaatgagaa
     3961 aggcgaagat gtggttgttc cgggactgtt tgccgttggt gaaatcgctt gtgtatcggt
     4021 acacggcgct aaccgtctgg gcggcaactc gctgctggac ctggtggtct ttggtcgtgc
     4081 ggcaggtctg catctgcaag agtctatcgc cgagcagggc gcattgcgcg atgccagcga
     4141 gtctgatgtt gaagcatctc tggatcgtct gaaccgctgg aacaataatc gtaacggtga
     4201 agatccggtg gcgattcgta aagcgctgca agaatgtatg cagcataact tctcggtctt
     4261 ccgtgaaggt gatgcgatgg cgaaagggct tgagcagttg aaagttatcc gcgagcgtct
     4321 gaaaaatgcc cgtctggatg acacttccag cgagttcaac acccagcgcg ttgagtgcct
     4381 ggaactggat aacctgatgg aaacggcgta tgcaacggct gtttctgcca acttccgtac
     4441 cgaaagccgt ggcgcgcata gccgcttcga cttcccggat cgtgatgatg aaaactggct
     4501 gtgccactcc ctgtatctgc cagagtcgga atccatgacg cgccgaagcg tcaacatgga
     4561 accgaaactg cgcccggcat tcccgccgaa gattcgtact tactaatgcg gagacaggaa
     4621 aatgagactc gagttttcaa tttatcgcta taacccggat gttgatgatg ctccgcgtat
     4681 gcaggattac accctggaag cggaagaagg tcgcgacatg atgctgctgg atgcgcttat
     4741 ccagctgaaa gagaaagatc ccagcctgtc gttccgccgc tcctgtcgtg aaggtgtgtg
     4801 cggttccgac ggtctgaaca tgaacggcaa gaatggtctg gcctgtatta ccccgatttc
     4861 ggcactcaac cagccgggca agaagattgt gattcgcccg ctgccaggtt taccggtgat
     4921 ccgcgatttg gtggtagaca tgggacaatt ctatgcgcaa tatgagaaaa ttaagcctta
     4981 cctgttgaat aatggacaaa atccgccagc tcgcgagcat ttacagatgc cagagcagcg
     5041 cgaaaaactc gacgggctgt atgaatgtat tctctgcgca tgttgttcaa cctcttgtcc
     5101 gtctttctgg tggaatcccg ataagtttat cggcccggca ggcttgttag cggcatatcg
     5161 tttcctgatc gatagccgtg ataccgagac tgacagccgc ctcgacggtt tgagcgatgc
     5221 attcagcgta ttccgctgtc acagcatcat gaactgcgtc agtgtatgtc cgaaggggct
     5281 gaacccgacg cgcgccatcg gccatatcaa gtcgatgttg ttgcaacgta atgcgtaaac
     5341 cgtaggcctg ataagacgcg caagcgtcgc atcaggcaac cagtgccgga tgcggcgtga
     5401 acgccttatc cggcctacaa gtcattaccc gtaggcctga taagcgcagc gcatcaggcg
     5461 taacaaagaa atgcaggaaa tctttaaaaa ctgcccctga cactaagaca gtttttaaag
     5521 gttccttcgc gagccactac gtagacaaga gctcgcaagt gaaccccggc acgcacatca
     5581 ctgtgcgtgg tagtatccac ggcgaagtaa gcataaaaaa gatgcttaag ggatcacgat
     5641 gcagaacagc gctttgaaag cctggttgga ctcttcttac ctctctggcg caaaccagag
     5701 ctggatagaa cagctctatg aagacttctt aaccgatcct gactcggttg acgctaactg
     5761 gcgttcgacg ttccagcagt tacctggtac gggagtcaaa ccggatcaat tccactctca
     5821 aacgcgtgaa tatttccgcc gcctggcgaa agacgcttca cgttactctt caacgatctc
     5881 cgaccctgac accaatgtga agcaggttaa agtcctgcag ctcattaacg cataccgctt
     5941 ccgtggtcac cagcatgcga atctcgatcc gctgggactg tggcagcaag ataaagtggc
     6001 cgatctggat ccgtctttcc acgatctgac cgaagcagac ttccaggaaa ccttcaacgt
     6061 cggttcattt gccagcggca aagaaaccat gaaactcggc gagctgctgg aagccctcaa
     6121 gcaaacctac tgcggcccga ttggtgccga gtatatgcac atcaccagca ccgaagaaaa
     6181 acgctggatc caacagcgta tcgagtctgg tcgcgcgact ttcaatagcg aagagaaaaa
     6241 acgcttctta agcgaactga ccgccgctga aggccttgaa cgttacctcg gcgcaaaatt
     6301 ccctggcgca aaacgcttct cgctggaagg cggtgacgcg ttaatcccga tgcttaaaga
     6361 gatgatccgc cacgctggca acagcggcac ccgcgaagtg gttctcggga tggcgcaccg
     6421 tggtcgtctg aacgtgctgg tgaacgtgct gggtaaaaaa ccgcaagact tgttcgacga
     6481 gttcgccggt aaacataaag aacacctcgg cacgggtgac gtgaaatacc acatgggctt
     6541 ctcgtctgac ttccagaccg atggcggcct ggtgcacctg gcgctggcgt ttaacccgtc
     6601 tcaccttgag attgtaagcc cggtagttat cggttctgtt cgtgcccgtc tggacagact
     6661 tgatgagccg agcagcaaca aagtgctgcc aatcaccatc cacggtgacg ccgcagtgac
     6721 cgggcagggc gtggttcagg aaaccctgaa catgtcgaaa gcgcgtggtt atgaagttgg
     6781 cggtacggta cgtatcgtta tcaacaacca ggttggtttc accacctcta atccgctgga
     6841 tgcccgttct acgccgtact gtactgatat cggtaagatg gttcaggccc cgattttcca
     6901 cgttaacgcg gacgatccgg aagccgttgc ctttgtgacc cgtctggcgc tcgatttccg
     6961 taacaccttt aaacgtgatg tcttcatcga cctggtgtgc taccgccgtc acggccacaa
     7021 cgaagccgac gagccgagcg caacccagcc gctgatgtat cagaaaatca aaaaacatcc
     7081 gacaccgcgc aaaatctacg ctgacaagct ggagcaggaa aaagtggcga cgctggaaga
     7141 tgccaccgag atggttaacc tgtaccgcga tgcgctggat gctggcgatt gcgtagtggc
     7201 agagtggcgt ccgatgaaca tgcactcttt cacctggtcg ccgtacctca accacgaatg
     7261 ggacgaagag tacccgaaca aagttgagat gaagcgcctg caggagctgg cgaaacgcat
     7321 cagcacggtg ccggaagcag ttgaaatgca gtctcgcgtt gccaagattt atggcgatcg
     7381 ccaggcgatg gctgccggtg agaaactgtt cgactggggc ggtgcggaaa acctcgctta
     7441 cgccacgctg gttgacgaag gcattccggt tcgcctgtcg ggtgaagact ccggtcgcgg
     7501 taccttcttc caccgccacg cggtgatcca caaccagtct aacggttcca cttacacgcc
     7561 gctgcaacac atccataacg ggcagggcgc gttccgcgtc tgggactccg tactgtctga
     7621 agaagcagtg ctggcgtttg aatacggtta tgccaccgca gaaccacgca ctctgaccat
     7681 ctgggaagcg cagttcggtg acttcgctaa cggtgctcag gtggttatcg accagttcat
     7741 ctcctctggc gaacagaaat ggggccggat gtgtggtctg gtgatgttgc tgccgcacgg
     7801 ttatgaaggg caggggccgg agcactcctc cgcgcgtctg gaacgttatc tgcaactttg
     7861 tgctgagcaa aacatgcagg tgtgcgtacc gtctaccccg gcacaggttt accacatgct
     7921 gcgtcgtcag gcgctgcgcg ggatgcgtcg tccgctggtc gtgatgtcgc cgaaatccct
     7981 gctgcgtcat ccgctggcgg tttccagcct cgaagaactg gcgaacggca ccttcctgcc
     8041 agccatcggt gaaatcgacg agcttgatcc gaagggcgtg aagcgcgtag tgatgtgttc
     8101 tggtaaggtt tattacgacc tgctggaaca gcgtcgtaag aacaatcaac acgatgtcgc
     8161 cattgtgcgt atcgagcaac tctacccgtt cccgcataaa gcgatgcagg aagtgttgca
     8221 gcagtttgct cacgtcaagg attttgtctg gtgccaggaa gagccgctca accagggcgc
     8281 atggtactgc agccagcatc atttccgtga agtgattccg tttggggctt ctctgcgtta
     8341 tgcaggccgc ccggcctccg cctctccggc ggtagggtat atgtccgttc accagaaaca
     8401 gcaacaagat ctggttaatg acgcgctgaa cgtcgaataa ataaaggata cacaatgagt
     8461 agcgtagata ttctggtccc tgacctgcct gaatccgtag ccgatgccac cgtcgcaacc
     8521 tggcataaaa aacccggcga cgcagtcgta cgtgatgaag tgctggtaga aatcgaaact
     8581 gacaaagtgg tactggaagt accggcatca gcagacggca ttctggatgc ggttctggaa
     8641 gatgaaggta caaccgtaac gtctcgtcag atccttggtc gcctgcgtga aggcaacagc
     8701 gccggtaaag aaaccagcgc caaatctgaa gagaaagcgt ccactccggc gcaacgccag
     8761 caggcgtctc tggaagagca aaacaacgat gcgttaagcc cggcgatccg tcgcttgctg
     8821 gctgaacata acctcgacgc cagcgctatt aaaggcaccg gcgtgggtgg tcgtctgacc
     8881 cgtgaagatg tggaaaaaca tctggcgaaa tccccggcga aagagtctgc accggcagcg
     8941 gctgctccgg cggcgcaacc ggctctggct gcacgtagtg aaaaacgtgt cccgatgact
     9001 cgcctgcgta agcgtgtggc agagcgtctg ctggaagcga aaaactccac cgccatgctg
     9061 accacgttca acgaagtcaa catgaagccg attatggatc tgcgtaagca gtacggtgaa
     9121 gcgtttgaaa aacgccacgg catccgtctg ggctttatgt ccttctacgt gaaagcggtg
     9181 gttgaagccc tgaaacgtta cccggaagtg aatgcttcta tcgacggcga tgacgtggtt
     9241 taccacaact atttcgacgt cagcatggcg gtttctacgc cgcgcggcct ggtgacgccg
     9301 gttctgcgtg atgtcgatac cctcggcatg gcagacatcg agaagaaaat caaagagctg
     9361 gcagtcaaag gccgtgatgg caagctgacc gttgaagatc tgaccggtgg taacttcacc
     9421 atcaccaacg gtggtgtgtt cggttccctg atgtctacgc cgatcatcaa cccgccgcag
     9481 agcgcaattc tgggtatgca cgctatcaaa gatcgtccga tggcggtgaa tggtcaggtt
     9541 gagatcctgc cgatgatgta cctggcgctg tcctacgatc accgtctgat cgatggtcgc
     9601 gaatccgtgg gcttcctggt aacgatcaaa gagttgctgg aagatccgac gcgtctgctg
     9661 ctggacgtgt agtagtttaa gtttcacctg cactgtagac aggataaggc attatcgcct
     9721 tctccggcaa ttgaagcctg atgcgacgct taacgcgtct tatcaggcct acgggaagca
     9781 ccaatgtagg tcggataagg cgcttgcgcc gcatccgaca agcgatgcct gatgcgacac
     9841 ttacgcgtct tatcaggcct acaggtgacc gacaatgccc ggaggcgata cgaaatattc
     9901 ggtctacggt ttaaaagata acgattactg aaggatggac agaacacatg aacttacatg
     9961 aatatcaggc aaaacaactt tttgcccgct atggcttacc agcaccggtg ggttatgcct
    10021 gtactactcc gcgcgaagca gaagaagccg cttcaaaaat cggtgccggt ccgtgggtag
    10081 tgaaatgtca ggttcacgct ggtggccgcg gtaaagcggg cggtgtgaaa gttgtaaaca
    10141 gcaaagaaga catccgtgct tttgcagaaa attggctcgg caagcgtctg gtaacgtatc
    10201 aaacagatgc caatggccaa ccggttaacc agattctggt tgaagcagcg accgatatcg
    10261 ctaaagagct gtatctcggt gccgttgtag accgtagttc ccgtcgtgtg gtctttatgg
    10321 cctccactga aggcggcgtg gaaatcgaaa aagtggcgga agaaactccg cagctgatcc
    10381 ataaagttgc gcttgatccg ctgacaggcc cgatgccgta tcagggacgc gagctggcgt
    10441 tcaaactggg tctggaaggt aaactggttc agcagttcac caaaatcttc atgggtctgg
    10501 cgaccatttt cctggagcgc gacctggcgt tgatcgaaat caacccgctg gtcatcacca
    10561 aacagggtga tctgatttgc ctcgacggca aactgggcgc tgatggcaac gcactgttcc
    10621 gccagcttga tctgcgcgaa atgcgtgacc agtcgcagga agatccgcgt gaagcacagg
    10681 ctgcacagtg ggaactgaac tacgttgcgc tggacggtaa catcggttgt atggttaacg
    10741 gcgcaggtct ggcgatgggt acgatggaca tcgttaaact gcacggcggc gaaccggcta
    10801 acttccttga cgttggcggc ggtgcaacca aagaacgtgt aaccgaagcg ttcaaaatca
    10861 tcctctctga cgacaaagtg aaagtcgttc tggttaacat cttcggcggt atcgttcgtt
    10921 gcgacctgat cgctgacggt atcatcggcg cggtagcaga agtgggtgtt aacgtaccgg
    10981 tcgtggtacg tctggaaggt aacaacgccg aactcggcgc gaagaaactg gctgacagcg
    11041 gcctgaatat tattgcagca aaaggtctga cggatgcagc tcagcaggtt gttgccgcag
    11101 tggaggggaa ataatgtcca ttttaattga taaaaacacc aaggttatct gccagggctt
    11161 tactggtagc caggggactt tccactcaga acaggccatt gcatacggca ctaaaatggt
    11221 tggcggcgta accccaggta aaggcggtac tacccacctc ggcctgccgg tgttcaacac
    11281 cgtgcgtgaa gctgttgctg ctactggcgc taccgcttct gttatctacg taccagcacc
    11341 gttctgcaaa gactccattc tggaagctat tgatgcaggc atcaaactga ttatcaccat
    11401 cactgaaggc atcccgacgc tggatatgct gactgtgaaa gtgaaactgg acgaagccgg
    11461 cgttcgtatg atcggcccga actgcccggg cgttatcact ccgggtgaat gcaaaatcgg
    11521 tatccagccg ggtcacattc acaaaccggg taaagtgggt atcgtttccc gttccggtac
    11581 actgacctat gaagcggtta aacagaccac ggattacggt ttcggtcagt cgacctgtgt
    11641 tggtatcggc ggtgacccga tcccaggctc taactttatc gacatcctcg aaatgttcga
    11701 aaaagatccg cagaccgaag cgatcgtgat gatcggtgag atcggcggta gcgctgaaga
    11761 agaagcggca gcgtacatca aagagcacgt taccaagcca gttgtaggtt acatcgctgg
    11821 tgtgactgcg ccgaaaggca aacgtatggg ccacgcgggt gcgatcattg ccggtgggaa
    11881 agggactgcg gatgagaaat tcgctgctct ggaagccgca ggcgtgaaaa ccgttcgcag
    11941 cctggcggat atcggtgaag cactgaaaac tgttctgaaa taaatatctg taataagaaa
    12001 tagccctcgc cgcttccctc tacaggaatg gcgaagggct gtcggtttcg acatggttgg
    12061 ccatcgtatg atggcctttt ttgtgcttat cgcgatgatt ttcgctgcgc tatcagggta
    12121 aatttatagt catcggtatt aaaagcgttg cggctatatt caaacacccg accatcaact
    12181 aaatatccac gcgatacttt ttcaagaatc ggctttgtct ggctgatatt aagcagacgg
    12241 ctcatctctt cggttggcat cagaggaatg atttcctgtt cgctacgatc gataaccatc
    12301 ttcttcactt cttcaataaa gtgatatttc gaattttcca tgacctgcca ggtgagatcc
    12361 gggaacaacg caagcggcat ccaggtttct tccagcgcca ttggcttttg cttgcgatag
    12421 cgcacgcgct tcacatgcca cacacgatcc tgcgcggtga tttgtagctg ttgctgaaga
    12481 aaatcgtcag ccggaatcac ttcaaatatc aggacttcac tgtgtgtatc gacgtgacgg
    12541 tccgacagtt tttcatcaaa actggttaac tgaaaaatat cgtaattgac ccgctcttct
    12601 ttgacgtaag tcccgctgcc ctgaatgctt tcgaggatct gctgctcgac tagctggcgc
    12661 aaagcctgac gcaccgtaac ccggctgacg ccaaactctg tttgtagcgc tgattcagtg
    12721 ggtaacgcat cgccaggttt aagctcgcca cgcgcaattt gttcacgaat gcgatcggca
    12781 atctgccggt ataagggctt gtgtcccatt tttagtatct cattaatacg aatttagcca
    12841 ttatgcccga taaattcatc ctgtaaataa tgcaaataca atacaaataa tttcaatcaa
    12901 gtgaaattga tcatatagtg gtattgtttt atcgggcaca ctggcgcgac tataaaaacg
    12961 atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg ttgcgtctga
    13021 atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt cttgctgctc
    13081 tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt gaaagccttg
    13141 gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
    13201 aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc gttgatggcc
    13261 cggaagcagt tgatttagtg gtgctgctgg cgattccccc caatgaagcg ggtactatgc
    13321 atatgcaact gctgacagcg ctgaccacgc gccttgcgga tgatgagatt cgggcgcgta
    13381 tacagtcggc gacgacgcct gatgagttgc tctcggcgct ggatgacaag ggaggcacgc
    13441 aaccttctgc ctctttttcc aacgcgccaa ctatcgtctg cgtaccggcc tgtccggcgg
    13501 gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc aaactcggcg
    13561 taaatgttta cgttgaaaaa caaggcgcta acggcattga agggcgttta acggcggatc
    13621 aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
    13681 gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
    13741 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
    13801 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
    13861 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg gtattactgt
    13921 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
    13981 gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg gcctataccg
    14041 cctattctct ggcagataaa ccggcgttag cgctaggctt tgcggctgga cttgccgcca
    14101 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
    14161 tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt caatggattc ctgacttttt
    14221 atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt gtggtgggag
    14281 aacctgtcgc atggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
    14341 acgcgctgtt gctgggtgcc attttcggtt ttatgtgttc ctttgatctt ggagggccag
    14401 tgaacaaagc cgcttatgca ttctgcctgg gcgcaatggt gaacggcgtt tacggcccgt
    14461 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
    14521 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
    14581 ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
    14641 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
    14701 gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
    14761 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
    14821 ctgcaattct cctgatctgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
    14881 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
    14941 cccgcatatg cactgggatc gagagtggta tttcaccacc gaagagtcac gtattctgct
    15001 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
    15061 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
    15121 agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
    15181 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
    15241 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccagatt cctttggcat
    15301 gtccgggcaa ctgccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
    15361 cggatgttcg gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
    15421 tagcgaagtg acggcgcagg tgctgccgct gggctacgcc ataggtaagt acttacctac
    15481 cgacgaaaac ggattacgta aacgcctcga cagttatttt gacgtgctgg aaaaagcgtc
    15541 tgtaaccaaa gagattttgc tgccgaatgg tcatgaccag atgccattgc agcaaaatat
    15601 cttcgaagtg atggataagc tacgtgagat ctaccctcaa cgtaagtttg tgatgagccg
    15661 ctttgaagag gtatttgaga agatcgaagc gcagcgtgaa agtctggcca ccctgaaagg
    15721 tgaatttatc gatggcaaat atatgcgcgt acatcgcacc atcggttcca cgcgcatgga
    15781 tatcaaaatt gcccacgcac gtattgaaaa taagattgtt aatctgctgg aaccgctggc
    15841 aacgctggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
    15901 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
    15961 tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgctgcgttt
    16021 ctacatgcgc aaaattgccg ataacatgcc acagagtgat gccgacaaac tcgtgctgtt
    16081 taacctgatg ccctggccgc gggaagaagt tattaacacc actgtgcggc tgcgcgccag
    16141 ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc gccatgcccg
    16201 tgagatcgat ccaggcctaa tcgatcggca aatagttcat tacggtaatt acgatccctt
    16261 tatggagttt gatatacaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
    16321 tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggatact
    16381 ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatggt tctctgcaac tggtagataa
    16441 agacagcggt gtgcgctatg accgagtatt gc
//
LOCUS       DADLTD010000087        16381 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLTD010000087 DADLTD010000000
VERSION     DADLTD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.087"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..190
                     /locus_tag="JN701_002191"
     CDS             <1..190
                     /locus_tag="JN701_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364637.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="JN701_002192"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="JN701_002192"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY7364638.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="JN701_002193"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="JN701_002193"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY7364639.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="JN701_002194"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="JN701_002194"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY7364640.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /locus_tag="JN701_002195"
     CDS             4544..4912
                     /locus_tag="JN701_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY7364641.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="JN701_002196"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="JN701_002196"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY7364642.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5649..7028
                     /gene="dinF"
                     /locus_tag="JN701_002197"
     CDS             5649..7028
                     /gene="dinF"
                     /locus_tag="JN701_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY7364643.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            7144..7353
                     /locus_tag="JN701_002198"
     CDS             7144..7353
                     /locus_tag="JN701_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY7364644.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="JN701_002199"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="JN701_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY7364645.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..8482
                     /locus_tag="JN701_002200"
     CDS             8228..8482
                     /locus_tag="JN701_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364646.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8506..9213
                     /locus_tag="JN701_002201"
     CDS             8506..9213
                     /locus_tag="JN701_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY7364647.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQTIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9576..10613
                     /gene="dusA"
                     /locus_tag="JN701_002202"
     CDS             9576..10613
                     /gene="dusA"
                     /locus_tag="JN701_002202"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY7364648.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10747..10989
                     /gene="pspG"
                     /locus_tag="JN701_002203"
     CDS             10747..10989
                     /gene="pspG"
                     /locus_tag="JN701_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY7364649.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11154..12137)
                     /locus_tag="JN701_002204"
     CDS             complement(11154..12137)
                     /locus_tag="JN701_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY7364650.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12220..13635
                     /locus_tag="JN701_002205"
     CDS             12220..13635
                     /locus_tag="JN701_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY7364651.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13688..14767
                     /gene="alr"
                     /locus_tag="JN701_002206"
     CDS             13688..14767
                     /gene="alr"
                     /locus_tag="JN701_002206"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY7364652.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15020..16213
                     /gene="tyrB"
                     /locus_tag="JN701_002207"
     CDS             15020..16213
                     /gene="tyrB"
                     /locus_tag="JN701_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY7364653.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         3914 a         3904 c         4336 g         4227 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat agtatatgta atcctgggga taataaagat
     8641 gcgactccca ttgaacaatg tacttttgat gtagaaacta agaatggcct tccctccacg
     8701 gttcagctat tttatgagga atctaaggat aatgaacctt tagcgaatat acattttcaa
     8761 acaataggtt ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc
     8821 ttaaaattat ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta
     8881 tcagaaacag tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa
     8941 ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc tgatggggga
     9001 gaaatgaatt ttgatggcta tctggataag atgtcacatc tggtgaatga agggacgctc
     9061 gatcctgata ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag
     9121 tcgtttgacc caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt
     9181 tatgatttgt tcggggttaa atcggagaaa taatatgtga agttcttcga tagtatggaa
     9241 ggcattatat aaaaggaccc aatatttatt gggtcctttt ttctctatca atgctattag
     9301 cagggagata tatcaccaaa gtttaatgtg tgatttttta tttatcgtcg aacctggatt
     9361 gtttatcatt ggccttaaca aagttaacgg ctaataagat tatttccatc acttcgtgag
     9421 agcttcatgc cttgagagga tctcaatttt cttttgcaat gagacaggcg cttcctgttg
     9481 ttatggtata gtaccccgct attgagcctc ctgaatagtg atgctgaata acataacccc
     9541 atgatatatc gataaaataa tctctacatt tgaaaatgca cggtaattct gaaatgcaaa
     9601 aaatcaacca aaccagcgca atgcctgaaa aaactgacgt tcactggagt ggtcggttta
     9661 gcgttgcacc aatgctcgac tggacggaca gacattgccg ctatttcttg cgtctgcttt
     9721 cccgcaatac gttgctgtat accgaaatgg tgaccacagg ggcgattatt cacggtaaag
     9781 gtgattacct ggcgtacagt gaagaagaac atccggtagc gttgcaactc ggcggtagcg
     9841 atccggcggc gctggcacag tgtgcgaagc tggcagaagc gcgtggatat gatgagatca
     9901 acctgaatgt cggctgcccg tctgaccggg tgcagaacgg catgtttggt gcgtgtctga
     9961 tgggtaatgc gcagctggtt gccgattgcg tgaaagcgat gcgcgatgtg gtgtcgattc
    10021 cggtgacggt gaaaacgcgt attggcatcg atgaccagga cagctatgaa tttctctgcg
    10081 atttcatcaa caccgtttcc ggcaaaggcg agtgtgggat gttcatcatc catgcacgta
    10141 aagcctggct ttcggggtta agcccgaaag aaaaccgtga aatcccgccg ctcgattatc
    10201 cgcgtgtgta tcaactgaag cgtgactttc cgcatctgac aatgtcgatt aacggtggta
    10261 tcaagtcgct ggaagaggcc aaagcacacc tgcaacatat ggatggcgtg atggtcgggc
    10321 gcgaggcgta tcagaatccg ggtattctgg cggcggtaga ccgggagatc tttggttcct
    10381 cggataccga tgcctatccg gtggcggtag tgcgcgccat gtatccgtac attgagcgtg
    10441 aactcagcca ggggacgtat ctcggccata ttacccggca tatgttgggc ttgttccagg
    10501 gtattcctgg cgcgcggcag tggcggcgtt atttaagtga aaatgcccat aaagcgggtg
    10561 cagacattaa tgtgctggaa cacgcgctca aactggtggc ggataagcgt taacttttca
    10621 ccaaaaagta gtcaaattca ccacgccctg cgcaccgtcg cggggcgttt tgctgttaaa
    10681 tcaatagatt atttttggca tgattcttgt aatgccagca agagatttca tatttgggag
    10741 agcatcatgc tggaactact ttttgtgatt ggcttttttg tcatgctgat ggtcaccggc
    10801 gtttcgttgc tgggcattat cgccgcgctg gttgtggcga cggccattat gttcctcggc
    10861 ggtatgctgg cattgatgat taagttgctg ccgtggttac tactggcgat tgcggtggtg
    10921 tgggttatta aggcgattaa agcaccaaaa gtgcctaaat atcagcgtta tgaccgctgg
    10981 cgttactaag ggattgtgcg gatgatcaca acctaaggtt ttatccttag aacaaaatag
    11041 gaattgataa tcaaatctgt cactattgcg cctctaacag attcatcgtg ctgtacccta
    11101 catacagccg actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga
    11161 atcagcaggc tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca
    11221 tccttcagcg gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc
    11281 aaagagaaca gttcattact ggcctcggtt aattcctccc gtgtggtgat atagccttgc
    11341 agggaagggc gtgtcacata caacgagcct ttttgattga gaatgcctaa gttcacaccg
    11401 gtaaccgcac ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc
    11461 agcgaccgtt cccaggtgtc tctgcccacg gaatcgtaca ctacgcgcac tttcttaccg
    11521 ccggtgatct ctttcaatcg ctcgaccaga ttctcttcac gatagttaat aacctgccac
    11581 gcgccggctt ttagcgcgct ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc
    11641 gcgcccagag cttttgccca ctggcaggca atcaagccaa cgccgccagc cgctgcgtgg
    11701 aacaggaact gttcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt
    11761 aagcctttca ggaaagatgc tgcagcttgc tcaaaagaaa tagccgcagg cagaatcgcc
    11821 gctttatccg cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag
    11881 actacacgat cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct
    11941 gccgcttcgg tacctgatcc gctgggtagt gatggcggcg ggtaaaggcc gctgcggata
    12001 tacgtgtcga taaaattgat gccgatggct ttattttcga cctggatttc attctctgcc
    12061 ggatcggcag gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat
    12121 tcaattcgtg ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa
    12181 agtttcgacc cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc
    12241 aacaaacagc aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct
    12301 ccgcactcga tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc
    12361 tgggacgatg tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgccat
    12421 atctttactg aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt
    12481 gcggaatcgc tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca
    12541 gagctgtcaa aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt
    12601 gaacgtgccg ttgtccgtga gatgatctcg gttgcgaatg agattgctga agccggtttt
    12661 gatccgcagg ggcgtaccag cgaagatctg ctggaccttg ctgaatcccg cgtctttaaa
    12721 attgccgaaa gtcgtgcaaa caaagacgaa gggccgaaga acatcgccga tgtgctcgac
    12781 gcaaccgtgg cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta
    12841 aacaccggtt atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc
    12901 atcgtcgccg cgcgtccgtc gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac
    12961 gcggcgatgt tgcaggataa accagtactt atcttctcgc tggagatgcc ttcagaacag
    13021 attatgatgc gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg
    13081 cagctcgatg atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa
    13141 cgcaatatct atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca
    13201 cgccgtattg cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg
    13261 atgcgcgtac cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctctcgctcg
    13321 ctgaaagcac tggcgaaaga actgaacgtg ccggtggtgg cgctgtccca gttgaaccgt
    13381 tctctggaac aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct
    13441 atcgagcagg atgcggactt gatcatgttt atctatcgtg atgaggtgta tcacgaaaac
    13501 agtgatttaa aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg
    13561 acggtacgcc tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag
    13621 tacgacgacg aataataatt attttatgaa ttaggcaatt aaagcaaaca cttatcaagg
    13681 aacacaaatg caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca
    13741 acgtcttcgt gaactggccc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta
    13801 tggtcacggt cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc
    13861 ccgtctcgaa gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact
    13921 cgaaggcttt tttgatgcca gagatctgcc gacgatttct gcgcaacatt ttcataccgc
    13981 cgtgcataac gaagaacagc tggctgcgct ggaagaggct agcctggacg agccggttac
    14041 cgtctggatg aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctgg
    14101 ggcgttttat catcgcctga cacagtgcaa aaacgttcgt cagccggtga atatcgtcag
    14161 ccattttgcg cgcgcggatg aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt
    14221 taataccttt tgcgaaggca aacctggtca acgttccatt gccgcgtcgg gtggcattct
    14281 gctgtggcca caatcgcatt ttgactgggt gcgcccgggc atcattcttt atggcgtctc
    14341 gccgctggaa gatcgctcca ccggtgccga ttttggctgt cagccagtga tgtcactaac
    14401 ctccagcctg attgccgtgc gtgagcataa agccggagag cctgttggtt atggtggaac
    14461 ctgggtaagc gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta
    14521 tccgcgcgcc gctccatccg gtacgccagt gctggtgaac ggtcgcgaag taccgattgt
    14581 cgggcgcgtg gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc
    14641 cggggatccg gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac
    14701 aaaagtgagc gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt
    14761 ggattaatcg ttctgtaata tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc
    14821 ggccaataaa atcctaaaaa ttcaataagt tgatgttctt tcatgctctt ataaaggtcg
    14881 tgcctctggc ggatgttcgt ttgtcatgag tctcactctg ttgctaattg ccgttcgctc
    14941 ctgaacatcc actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt
    15001 aaacctggag aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct
    15061 tacgcttatg gagcgtttta aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg
    15121 tctgtactac aacgaagacg gaattattcc acaactgaaa gccgtggcgg aggcggaagc
    15181 gcgcctgaat gcgcagcccc atggcgcttc gctttattta ccgatggaag ggcttaacag
    15241 ctatcgccat gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca
    15301 gcgcgtagca accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcggattt
    15361 cctgaaacgc tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca
    15421 cgtagcaata ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc
    15481 gactaacagc gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag
    15541 tattgtgttg ctgcatccat gttgccacaa cccaacaggt gccgatctca ctaatgatca
    15601 gtgggatgcg gtgattgaaa ttctcaaagc ccgcgagctt atcccattcc tcgatattgc
    15661 ctatcaagga tttagtgccg gtatggaaga ggatgcctac gctattcgcg ccattgccag
    15721 cgctggatta cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga
    15781 gcgcgtcggc ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg
    15841 gcaattgaaa gcaacagttc gccgcaacta ctccagcccg ccgaatttgg gtgcgcaggt
    15901 ggtggctgca gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga
    15961 gatgcgtact cgcattctgg caatgcgtca ggaattggtg aaggtattaa gcacagagat
    16021 gccagaacgc aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt
    16081 aagtgccgct caggttgacc gactacgtga agaatttggt gtctatctca tcgccagcgg
    16141 tcgcatgtgt gtcgccgggt taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc
    16201 tgcggtgatg taatgcagga aagcaggctg gagttaccca gcctgcagtg aaattaaact
    16261 gtcgtcgctt tcactatttc tttgtagatg atttttttga tgccgttgtt ctacgtgaga
    16321 gataataaaa cgttgtcagt tcttttattg ttaagtttat cccaattaac tggaattcct
    16381 t
//
LOCUS       DADLTD010000088        16161 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.088, whole genome shotgun sequence.
ACCESSION   DADLTD010000088 DADLTD010000000
VERSION     DADLTD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.088"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1101
                     /locus_tag="JN701_002208"
     CDS             <1..1101
                     /locus_tag="JN701_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAY7364654.1"
                     /translation="EKVVGLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAI
                     KGVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRA
                     ALMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGER
                     ETLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGN
                     RIGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREV
                     MDIDEAREKYASGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARL
                     RFGATWRVSPSDRLLNDLRGLIGSEQVELEFD"
     gene            1114..2073
                     /gene="accA"
                     /locus_tag="JN701_002209"
     CDS             1114..2073
                     /gene="accA"
                     /locus_tag="JN701_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY7364655.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSSYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2172..4281
                     /gene="ldcC"
                     /locus_tag="JN701_002210"
                     /pseudo
     CDS             2172..4281
                     /gene="ldcC"
                     /locus_tag="JN701_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            4338..4727
                     /locus_tag="JN701_002211"
     CDS             4338..4727
                     /locus_tag="JN701_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7364656.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4792..6090
                     /gene="tilS"
                     /locus_tag="JN701_002212"
     CDS             4792..6090
                     /gene="tilS"
                     /locus_tag="JN701_002212"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY7364657.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6139..6399)
                     /gene="rof"
                     /locus_tag="JN701_002213"
     CDS             complement(6139..6399)
                     /gene="rof"
                     /locus_tag="JN701_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY7364658.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6386..6586)
                     /locus_tag="JN701_002214"
     CDS             complement(6386..6586)
                     /locus_tag="JN701_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY7364659.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6752..7297
                     /locus_tag="JN701_002215"
     CDS             6752..7297
                     /locus_tag="JN701_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY7364660.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            7294..7716
                     /gene="arfB"
                     /locus_tag="JN701_002216"
     CDS             7294..7716
                     /gene="arfB"
                     /locus_tag="JN701_002216"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7364661.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            7730..8440
                     /gene="nlpE"
                     /locus_tag="JN701_002217"
                     /gene_synonym="cutF"
     CDS             7730..8440
                     /gene="nlpE"
                     /locus_tag="JN701_002217"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY7364662.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8640..9460)
                     /locus_tag="JN701_002218"
                     /pseudo
     CDS             complement(8640..9460)
                     /locus_tag="JN701_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(9513..11231)
                     /gene="proS"
                     /locus_tag="JN701_002219"
     CDS             complement(9513..11231)
                     /gene="proS"
                     /locus_tag="JN701_002219"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY7364663.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11342..12049)
                     /gene="tsaA"
                     /locus_tag="JN701_002220"
     CDS             complement(11342..12049)
                     /gene="tsaA"
                     /locus_tag="JN701_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY7364664.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(12046..12450)
                     /gene="rcsF"
                     /locus_tag="JN701_002221"
     CDS             complement(12046..12450)
                     /gene="rcsF"
                     /locus_tag="JN701_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY7364665.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12568..13383)
                     /gene="metQ"
                     /locus_tag="JN701_002222"
     CDS             complement(12568..13383)
                     /gene="metQ"
                     /locus_tag="JN701_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY7364666.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13423..14076)
                     /gene="metI"
                     /locus_tag="JN701_002223"
     CDS             complement(13423..14076)
                     /gene="metI"
                     /locus_tag="JN701_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY7364667.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(14069..15100)
                     /gene="metN"
                     /locus_tag="JN701_002224"
     CDS             complement(14069..15100)
                     /gene="metN"
                     /locus_tag="JN701_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY7364668.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15288..15860
                     /gene="gmhB"
                     /locus_tag="JN701_002225"
     CDS             15288..15860
                     /gene="gmhB"
                     /locus_tag="JN701_002225"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY7364669.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         3938 a         4073 c         4326 g         3824 t
ORIGIN      
        1 gagaaggtgg tgggcctggt ggatgagtgc tggcggatgg ggctgaaaat cctgccacca
       61 gatataaact ccggtcttta ccatttccac gtcaacgacg acggcgaaat cgtgtatggt
      121 attggcgcca tcaaaggggt aggtgaaggt ccgattgagg ccatcatcga agcccgtaat
      181 aaaggcggct acttccgcga actgtttgat ctctgcgccc gtaccgacac caaaaagtta
      241 aaccggcggg tgctggaaaa actgatcatg tccggggcgt ttgaccgtct tgggccacac
      301 cgcgcggcgc tgatgaactc gctgggtgat gcgttaaaag cggcagatca acacgcgaaa
      361 gcggaagcta tcggtcaggc cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt
      421 gaacaatcct acgccagctg ccaaccgtgg ccggagcagg tggtgttaga tggggaacgt
      481 gaaacgttag gtctgtacct tacgggacac cctatcaacc agtatttaaa agagattgag
      541 cgttatgtcg gaggcgtaag gctgaaagac atgcacccga cagaacgtgg taaagtcatc
      601 acggctgcgg ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc
      661 ggtatctgca cgctggatga ccgttccggg cggctggaag tgatgttgtt tactgacgcc
      721 ctggataaat accagcaatt gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc
      781 agctttgatg acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat ggatattgac
      841 gaagcccggg aaaaatatgc tagcgggctt gctatctcgc tgacggacag gcaaattgat
      901 gaccagcttt taaaccgact ccgtcagtct ctggaacccc accgctctgg gacaattcca
      961 gtacatctct actatcagag ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg
     1021 cgtgtctctc cgagcgatcg tttattaaac gatctccgtg gcctcattgg ttcggagcag
     1081 gtggaactgg agtttgacta atacaggaat actatgagtc tgaatttcct tgattttgaa
     1141 cagccgattg cagagctgga agcgaaaatc gattctctga ctgcggttag ccgtcaggat
     1201 gagaaactgg atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa
     1261 ctgacacgta aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact ggcacgccat
     1321 ccacagcgtc cttataccct ggattacgtt cgcctggcat ttgatgaatt tgacgaactg
     1381 gctggcgacc gcgcgtatgc agacgataaa gctatcgtcg gtggtatcgc ccgtcttgac
     1441 ggtcgtccgg tgatgatcat tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc
     1501 cgtaactttg gtatgccagc gccagaaggt taccgcaaag cactgcgtct gatgcaaatg
     1561 gctgaacgct ttaagatgcc tatcatcacc tttatcgaca ccccgggggc ttatcctggc
     1621 gtgggcgcag aagagcgtgg tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct
     1681 cgcctcggcg taccggtagt ttgtacggtt atcggtgaag gtggttctgg cggtgcgctg
     1741 gcgattggcg tgggcgataa agtgaatatg ctgcaataca gctcctattc cgttatctcg
     1801 ccggaaggtt gtgcgtccat tctgtggaag agcgccgaca aagcgccgct ggcggctgaa
     1861 gcgatgggta tcatcgctcc gcgtctgaaa gagctgaaac tgatcgactc catcatcccg
     1921 gaaccgctgg gcggcgctca ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa
     1981 ctgctggcgg atctggccga tctcgatgtg ttaagcactg aagatttaaa aaatcgtcgt
     2041 tatcagcgcc tgatgagcta cggttacgcg taattcgcaa aagttctgaa aaagggtcac
     2101 ttcggtggcc cttttttatc gccacggttt gagcaggcta tgattaagga aggattttcc
     2161 aggaggaaca catgaacatc attgccatta tgggaccgca tggcgtcttt tataaagatg
     2221 agcccatcaa agaactggag tcggcgctgg tggcgcaagg ctttcagatt atctggccac
     2281 aaaacagtgt tgatttgctg aaatttatcg aacataaccc tcgaatttgc ggcgtgattt
     2341 ttgactggga tgagtacagt ctcgatttat gcagcgatat caatcaactt aatgaatatc
     2401 tcccgcttta tgccttcatc aacacccact cgacgatgga tgtcagcgtg caggatatgc
     2461 ggatggcgct ctggtttttt gaatatgcgc tggggcaggc ggaagatatc gccattcgta
     2521 tgcgtcagta caccaacgaa tatcttgata acattacgcc gccgttcacg aaagccttgt
     2581 ttacctacgt taaagagcgg aagtacacct tttgtacgcc ggggcatatg ggcggcaccg
     2641 catatcaaaa aagcccggtt ggctgcctgt tttatgattt tttcggcggg aataccctca
     2701 aggctgatgt ctctatttcg gtcaccgagc ttggttcgtt gctcgaccac accgggccac
     2761 acctggaagc ggaagagtac atcgcgcgga cttttggcgc ggaacagagt tatatcgtta
     2821 ccaacggaac atcgacgtcg aacaaaattg tgggtatgta cgccgcgcca tccggcagta
     2881 cgctgttgat cgaccgcaat tgtcataaat cgctggcgca tctgttgatg atgaacgatg
     2941 tagtgccagt ctggctgaaa ccgacgcgta atgcgttggg gattcttggt gggatcccgc
     3001 accgtgaatt tactcgcgac agcatcgaag agaaagtcgc tgccaccacg caggcacaat
     3061 ggccggttca tgcggtgatt accaactcca cctatgatgg cttgctctac aacaccgact
     3121 ggatcaaaca gacgctggat gtcccgtcga tccacttcga ttccgcctgg gtgccgtaca
     3181 cccattttca tccgatctac caggggaaaa gtgatcttcg aaacgcaatc gacccacaaa
     3241 atgctggcgg cgttatcgca ggcttcgctg atccacatta aaggcgagta tgacgaagag
     3301 gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc cattgttgct
     3361 tcggttgaga cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt
     3421 tcggtggagc gggcgctgca ttttcgcaaa gaggtccagc ggttgcggga agagtctgac
     3481 ggttggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg ctggcccgtt
     3541 gcgccaggcg aacagtggca cggctttaac gatgcggatg ccgatcatat gtttctcgat
     3601 ccggttaaag tcactatttt gataccgggg atggacgaac agggcaatat gagcgaggag
     3661 gggatcccgg cggcgctggt ggcaaaattc ctcgacgaac gtgggatcgt agtagagaaa
     3721 accggccctt ataacctgct gtttctcttt agtattggta tcgataaaac caaagcaatg
     3781 ggattattgc gtgggttgac ggaatttaaa cgctcttacg atctcaacct gcggatcaaa
     3841 aatatgctac ccgatctcta tgctgaagat cccgatttct accgcaatat gcgtattcag
     3901 gatctggcgc aagggatcca taagctgatt cgtaaacacg atcttcccgg tttgatgttg
     3961 tgggcattcg atactttgcc ggaaattatc atgacgccac atcaggcatg gcagagacag
     4021 attaaaggtg aagtagaaac cattgcgctg gaacaactgg tcggtagagt atcggcaaat
     4081 atgatcctgc cttatccacc gggcgtaccg ctgttgatgc caggagaaat gctgaccaaa
     4141 gagagccgca cggtactcga ttttctactg atgctttgtt ccgtagggca acattacccc
     4201 ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggcgttta ccgcgtacga
     4261 gtcctaaaaa tggcgggata acttgccaga gcggcttacg ggcgagtaac gtgctgttaa
     4321 caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg attattgcga
     4381 cggattatgc ggtgagcaaa gctttctact gcgatattct tggtttcacg ctgcaaagcg
     4441 aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat gggcaatatg
     4501 tgattgagct tttctctttt ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg
     4561 gcctgcgtca tctggctttt agcgttgatg acatcgatgc ggcagtggcg caccttgaaa
     4621 gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa cgcttcacct
     4681 tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc
     4741 cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat catgacactc
     4801 acgctcaata gacaacttct cacctcacgc cagattttgg tcgcctttag cggcgggctt
     4861 gactccaccg ttctgctgca tcagttggtg cagtggcgga cggaaaatcc gggcgtcact
     4921 ctgcgcgcta ttcatgtgca tcacggttta agtgccaatg ccgatgcctg ggttacgcat
     4981 tgcgaaaacg tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt acaacttgcg
     5041 caagaggggc tggggattga ggcccaggcg cggcaggcgc gttatcaggc atttgcccgt
     5101 accctattgc ccggcgaagt gctggtcacc gcgcaacatc tcgacgatca atgtgaaacc
     5161 tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat ggcggaagtt
     5221 tcggagtttg ccggaacacg gcttatccgc ccgttgcttg cccgcacgcg gggggaactg
     5281 gcgcagtggg cattggcgca tggtttacgc tggattgaag acgaaagtaa tcaggacgac
     5341 tcatacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca gcgttggccg
     5401 cattttgcgg aagcaacggc ccgcagcgcc gcactttgtg ctgaacaaga gagtctgttg
     5461 gatgaactgc tggcggatga tttagcacac tgccaaacgt cgcaggggac gctgcagatt
     5521 gcgccaatgc tggcgatgag tgatgcccgc cgcgcggcga ttatccgtcg ctggctggca
     5581 gggcagaatg caccaatgcc ttcccgcgac gcgctggtgc ggatctggca ggaagtggcg
     5641 ctggcgcggg aagatgcctc cccctgttta cgcttgggcg cgtttgaaat ccgccgctat
     5701 cagtcgcaac tgtggtggat taaatccgtc accgggcaaa gtgaaaccat tgtgccgtgg
     5761 cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaagcgtaca gcttactgcg
     5821 ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt caaatcggca
     5881 ggattgctgc atattgtcgg gcgcaacggc ggacgtaagc tgaagaaaat ctggcaagag
     5941 ttaggcgtgc cgccgtggct acgtgacacc acgccactgc tgttttatgg cgaaacgctg
     6001 attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgaaggtga gaatggcgta
     6061 agttttgtct ggcagaaaac gcttagttaa gtgaaagccg gataagacgc atcaaacgtc
     6121 gcatccggcg aaagtcaatc aggactcgct caccaccacc gtaccgattt ccgggtggct
     6181 aaagctggta attttatcca gacgtagctc acgggtttcg ccagcggcct cgacgaccag
     6241 atactccaca tttttgcggg agactaaatc gctggctttt gcctgcaatt tttcgccatc
     6301 tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc
     6361 atcacaattg attggttgat acgtatcatt cattaacata atcgctcacc agtaagtttg
     6421 ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg gcagccattt
     6481 cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag tctccgctgg
     6541 cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc cctcctgtca
     6601 gcacactgac ttaaccgtgg atgcaagtct aagcctacga agataaactc tgtttcgcaa
     6661 ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt attccgttac
     6721 aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa tttataaagc
     6781 gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc
     6841 gcgccaccct tcagaaaccc aggagcgtat gatgctgcgc ctgctggcgt ggctgaaata
     6901 tgctgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg
     6961 gctgcgtaac gatcacctgg gcattgattt gtggattgag ctggggctgc cggatgagcg
     7021 gcggattaag aaagcctgca ctcaggccgc agaagtggcg ctgtttacct ataatagtcg
     7081 ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gtgcagtttg ccaatctttc
     7141 cgtctggtat ctggacgatg aacaactggc gaaagtaagc gcctttgccg atcgtaccat
     7201 gacgctgcag gcaacgattc aggatggcgt gatctggtta tcggacgata agaataatct
     7261 ggaagttaac ttaaccgcct ggcaacaacc ttcatgattg tgatttcccg acatgttgct
     7321 attcccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat
     7381 gttaataaga cctcaacggc cattcatctg cgttttgaca ttcgggcgtc cagcctgcca
     7441 gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag tgatggcgtg
     7501 attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc cgcgctagcc
     7561 cggctggtgg cagtgattaa agatttaaca acagaacaaa aagcccgacg acccacgcgg
     7621 cccacccgtg catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc aagcgcgaag
     7681 gcgatgcgcg gcaaagtacg cagcggtcgg gaataaaaag aaggaataga tggtgaaaaa
     7741 agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat gtaataaccg
     7801 ggccgaagtc gatacgcttt ctccagcaca ggctgccgaa ctgaaaccga tgccgcaaag
     7861 ttggcgcggc gtgctgccgt gtgctgattg tgaaggaatc gaaacctctc tgttcctcga
     7921 aaaagacgga acatgggtga tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc
     7981 cttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa
     8041 aggtgaaaag tcatattatc gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg
     8101 caatccgatt gaatcgcagt tcaactatac gctggaaccg gcacaatcca gtttacctat
     8161 gacgccgatg accctgcgag gcatgtattt ttatatggct gatgcggcga ccttcactga
     8221 ttgcgcgacc ggaaaacgtt tcatggtagc gaataacgca gagctggagc gtggctacct
     8281 ggctgcgcgc ggtcacagtg aaaaaccggt gttactgtca gtagaaggtc actttacgct
     8341 tgaggctaat ccggatactg gtgcgccaac caaagtatta gcgcccgata cggcgggtaa
     8401 attttacccg aataaggatt gcagtagttt ggggcagtaa acctgcttaa ggcagaaaca
     8461 aacgcaaaac aggccagaag gatatatttc aacattttga atttgcacgt tttttgtagg
     8521 ccggataagg cgtttacgcc gcatccggca atggtgctca acgcctgatg cgacgctggc
     8581 gcgtcttatc atgcctacaa cccccctcat accttaaggc ggataaggca tttactttat
     8641 caccgggtca aaccaacaaa gcgacccgcc ttgatataaa tccctggctt caggtgccca
     8701 acatactgta actgcgtttc tggcttaaac gcagagacat caccaatacg catatgcatc
     8761 agatcggcag ggctaatcca gcccgattgc gccagtgtaa gtggatgtcc ggctttggca
     8821 aatgcatcag tgacaaattc cgaacaaaac cacgactttt tgtctccttc gcccacactg
     8881 cttaactgcg ctttcgccag gccgctgacg cactgctggc gaaaatcctc ggagaactga
     8941 ttcagtgagc acatctggcg agtcaccata aagggaataa attcgacaat gccgcgatag
     9001 ttataaccgc tatctttgat tttattggca aacgcggtga tatctgtggc ttgttgcggg
     9061 gtaagatccg ggactcgtaa gacgaaaagc ttatcactat gcttcatcgc ttttttaagg
     9121 gaaacaatct ggacgccagc gcctgtcgct tctgcaacgt tattatcacc cagaaagatt
     9181 gcaacgtgac tcacagagga agtgctgaag acgcggattc caaatgaggt taccccaagg
     9241 cttgaggaga acagcaaatc gccgggtttg agatcaggtg ccgttatttc ttttattgat
     9301 tgttcggtga aagagctttg atgctggaat ttgacagccc atgtttttgc ctcggcatct
     9361 actgctgtgg ctgatggggc tggctgatat caacggtaca agccgaaagt aaaagaaagc
     9421 tggggagaag caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa
     9481 aagggccctg aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat
     9541 tcgacgatgt caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg
     9601 atatcgtcgt tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt
     9661 tccatatcag caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata
     9721 ccttgtgcac gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg
     9781 tgcatgttca tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg
     9841 ccgcgttcgt cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg
     9901 tagcaaccca tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt
     9961 gcttcggagt acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc
    10021 agcagcgtac cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca
    10081 acttccgggg tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg
    10141 ttagcaccag cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc
    10201 ggcatgttta ccggacccag tgaacccgga ccggctttaa ccacagcacg aatttcttct
    10261 tcggtcgcga aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc
    10321 agctcgtgat caccgcgcac cagcagcgca accagcggga agctgctgcc ttcaaccgct
    10381 ttaaccagca gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc
    10441 gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc
    10501 ggttctttcg gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg
    10561 gagaagacca catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg
    10621 ccgccgatag aaccggtgtc ggcttgcacg gcgcggaaat ccagccccat gcggctgaag
    10681 attttgctgt aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta
    10741 tggaaagagt aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga
    10801 cgcacttcgt cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa
    10861 gagctaagct cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg
    10921 aacggacgct cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga
    10981 ccactctctt gccacaaatc ggctggctga accaccggca tcgacacctc gattgcaccg
    11041 gcgttgttca tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc
    11101 ggcagccagg tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc
    11161 tgatggctga tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg
    11221 ctagtacgca tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg
    11281 gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa
    11341 tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt
    11401 taaaatcatg tagccagacg gcataggttt tgcccgtttc ctcaccttta tgataggccg
    11461 ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct
    11521 ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgcagctg
    11581 gcgcactttg cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg
    11641 gtttgatatc cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca
    11701 cgctgtcttt atggcaaaca acctctttca gctctaccag cgacatacca attgggttag
    11761 ggcggaaagt agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg
    11821 gacgcacagt cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata
    11881 aatggctgaa cgcttccagg ccgcgaacgg cgtcggcctg gttgtaggga gcaatgagat
    11941 gcagttcgcc gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt
    12001 tatagggcga gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat
    12061 gttaagcgca gagccgatac atacagcctg acgatagcag cctggcgtac cgctggtgac
    12121 ttcgcagcta tgcagtaata ccgcattggc tttcatttta gaggcgttga tttgcatccg
    12181 cttacgcgcg gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc
    12241 actgacttca ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat
    12301 tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc
    12361 agtgctttga acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag
    12421 tgctactaaa cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc
    12481 aatattgaat caggagcttg taaaaatgac aagacgggca agtgcccatc ctgaatgata
    12541 ttacaaattg tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac
    12601 tttgtttgct gcttcgtaaa cttcatcaga ctgataagcc tggacgaatt tcttcacgtt
    12661 ctcggcgtct ttgttatctt cacgcgttac gatcaggttt acgtacgggg agtctttatc
    12721 ttcgacaaag ataccgtctt tcgctggagt caggccaatc tggctggcat aggtggtatt
    12781 gataactgcc agagcgattt gtgcgtcgtc cagagatcga ggcagctgcg gtgcttccag
    12841 ctcaacaatt ttcagatttt ttgggttctc aacaacatca agtacggtcg gcagcaggcc
    12901 aacgccatct ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag
    12961 gttagttgga tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt
    13021 gattttcttg gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt
    13081 gtagccacga tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc
    13141 gatatcgcct ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc
    13201 aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg acttctgcaa cctgctgttc
    13261 ggcaccaaca atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc
    13321 taccagtgcc agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc
    13381 cataccttat tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc
    13441 ccggacgatg cggtcgcctg cgaactgaat taaataaacc agaatgacca gcaataccag
    13501 taccgtattc atcaccgtcg cgttatagcc gatgtagcca tactgatagc caatctgacc
    13561 taaaccaccg gcaccgacag caccacccat cgcggaataa ccgactaggg taatcagggt
    13621 gatagttgcc gcattcacca gacccggcaa tgcttccggt aacagcactt tacggacgat
    13681 ctgcatcggc gtggcaccca ttgcacggga agcttcaatt aacccggttg ggatctccag
    13741 cagagcgttc tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat
    13801 cgctgcctgc aaaccaatcg atgtaccaac aataacgcgg gtaaacggaa tcatccatac
    13861 cagcaagata atgaacggga tagaacggaa aatgttcaca atcgcagaaa cggtacgata
    13921 cagcttcgcg ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg
    13981 cagaccaatc acaaagccaa aaaaaccgga tacgaaggtc attgccagcg tttcccatac
    14041 gccacgaacc agcagccaca tcatcggctc agacataacc cagtacctct acttttacat
    14101 ggtgttcctg cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag
    14161 tcagcatgat gccgaactta acgccaccgg cgtagtccat ctgcgcgcta ataatgttgt
    14221 tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg
    14281 taaactccag acgcagcatc ggcacgcagt cagtaaatgg ctccgcttgc agacgttcct
    14341 ggtaatcttc cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg
    14401 ttttcggatg cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga
    14461 tgaccgccac gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa
    14521 tcgtcaaacc cagacggcgg ttaatgtctt tcagcagttc gagaatagaa cgtgtcgttg
    14581 ccgggtccag cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg
    14641 cacgggcaat cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat
    14701 gcttatcgcc aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt
    14761 tcggtgtgtt gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga
    14821 gcaggttaaa atgctggaaa atcataccaa tctggcggcg agctttggtc aactcggatt
    14881 ctgacagcgt ggtcagttcc tggccatcga ccagcacgct accctcggtt gggcgctcca
    14941 gcaggtttac acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat
    15001 aaatttgtcc agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc
    15061 cctggtggaa cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat
    15121 taagttagtc gtggcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt
    15181 taaggcatcc agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg
    15241 aaacatgcga tactagcgtc acatgcctta ttaaggagct ataaaaggtg gcaaagagcg
    15301 tacccgcaat ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg
    15361 agatcgacaa ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa
    15421 tgggctttgt gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg
    15481 aagcacagtt tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg
    15541 atctggatgg tatctattat tgcccgcatc atccgcaggg tagtgttgaa gagtttcgcc
    15601 aggtctgcga ctgccgtaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc
    15661 atattgatat ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg
    15721 ctgcggcgaa cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acgcctgaag
    15781 cagaaaacgc ggcggattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa
    15841 agcagcaaaa accggcataa tgattaaaag atgagcgatt gaaataaaaa tgcatttttc
    15901 cacttgtctt cctgagacga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat
    15961 cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca gggttgactc
    16021 tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagtg cgtcgcaact
    16081 gctctttaac aatttatcag gcaatctgtg tgggcactcg aagatacgga ttcttaacgt
    16141 cgcaagacga aaaatggata c
//
LOCUS       DADLTD010000089        16129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLTD010000089 DADLTD010000000
VERSION     DADLTD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.089"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..856)
                     /locus_tag="JN701_002226"
     CDS             complement(<1..856)
                     /locus_tag="JN701_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7364670.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVD
                     KIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDS
                     VDTEYIAKT"
     gene            complement(956..1867)
                     /gene="gsiD"
                     /locus_tag="JN701_002227"
     CDS             complement(956..1867)
                     /gene="gsiD"
                     /locus_tag="JN701_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY7364671.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILFIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1870..2790)
                     /gene="gsiC"
                     /locus_tag="JN701_002228"
     CDS             complement(1870..2790)
                     /gene="gsiC"
                     /locus_tag="JN701_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY7364672.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2808..4346)
                     /gene="gsiB"
                     /locus_tag="JN701_002229"
     CDS             complement(2808..4346)
                     /gene="gsiB"
                     /locus_tag="JN701_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY7364673.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKETKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASQSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4366..6237)
                     /gene="gsiA"
                     /locus_tag="JN701_002230"
     CDS             complement(4366..6237)
                     /gene="gsiA"
                     /locus_tag="JN701_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY7364674.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6224..7189)
                     /gene="iaaA"
                     /locus_tag="JN701_002231"
     CDS             complement(6224..7189)
                     /gene="iaaA"
                     /locus_tag="JN701_002231"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7364675.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7393..8628
                     /gene="moeA"
                     /locus_tag="JN701_002232"
     CDS             7393..8628
                     /gene="moeA"
                     /locus_tag="JN701_002232"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY7364676.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNHLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8628..9377
                     /gene="moeB"
                     /locus_tag="JN701_002233"
     CDS             8628..9377
                     /gene="moeB"
                     /locus_tag="JN701_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY7364677.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9453..10115)
                     /gene="fsa"
                     /locus_tag="JN701_002234"
     CDS             complement(9453..10115)
                     /gene="fsa"
                     /locus_tag="JN701_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7364678.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10246..11145
                     /locus_tag="JN701_002235"
     CDS             10246..11145
                     /locus_tag="JN701_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY7364679.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            11151..13583
                     /locus_tag="JN701_002236"
     CDS             11151..13583
                     /locus_tag="JN701_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY7364680.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13729..14544
                     /gene="ybiV"
                     /locus_tag="JN701_002237"
     CDS             13729..14544
                     /gene="ybiV"
                     /locus_tag="JN701_002237"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY7364681.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14696..15961
                     /locus_tag="JN701_002238"
     CDS             14696..15961
                     /locus_tag="JN701_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY7364682.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWIALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3951 a         4412 c         4052 g         3714 t
ORIGIN      
        1 atgtctttgc aatatactcc gtatcgacag aatcaataat attataatta ccttcgctat
       61 tataaataat cagtttttca cgagcgagtt gattcttggc aatgatctca tgaccttgtt
      121 tcaaaaatag aacttcatca ttagcgctga tttgatcaag atactcgttc aaataccgcc
      181 agtttaaatc gacatgaatt ttatctaccg ctaacaaatc gtgatctttg ccgttgatac
      241 gtagcgtaaa agagatacat tttttacccg gacgatcaaa gcaattgtat gccggattcc
      301 agctgttgtt agagtcatct gaagccaccg accacggtcg tttcgatggg ttaaaatcgg
      361 caaaaataac ccgccctgac tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa
      421 caacgatttt atcattcgcc ctccgtgaaa agagataata tttattatca tcaagtacca
      481 gtcccaccga gtctaaaaat ggcatcattt ccattttcag aatcacggcg cgttgaatct
      541 cctctgtatt ttttaataaa tacagtgagt cctttaatgt cagggccgaa cgttcaatat
      601 ctttaaagaa atagccaacg tttttctgaa taagttcaaa gcggctatcg gcacgtttta
      661 ccgcgcgttc cgataccagt tgatagttaa tgatgaaaaa aagaccaata aatgacagtg
      721 ctgccaataa aaataaaatt atcagcctta tctttatctt ttcgtataaa ctcagcatgt
      781 taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct
      841 gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac aacacttgct
      901 tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa cgtaactatc
      961 ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg aacgccagta
     1021 cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata accatatccg
     1081 ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca ccgaggccga
     1141 gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg aaaaacacca
     1201 cgatagacga gacggtccca ggcaagatat gacgcaataa aatggtcata tcgctggcac
     1261 caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca
     1321 ccagtcgggc aaacgcgggg atggaaaaaa tggcgactgc gataatcacg ttagcaatgc
     1381 cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag gcaaacagca
     1441 catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca gccaacaagc
     1501 ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc gccagcgaga
     1561 tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc
     1621 agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt tcggcgtcat
     1681 agggagcgat ccagcgtgca aaaatggcca ccacaataaa taaaataacg aataatgcgg
     1741 cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgca
     1801 cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata
     1861 gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca catccaccac
     1921 taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta
     1981 atcgcgcatt tctacggagt caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt
     2041 ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca tggtcactac
     2101 cgggatcatc gcgttacgta gcccgtgttt gaggacaacc caggtttcgc tcaccccttt
     2161 cgccctcgcg gtacgcatat aatcttcgct taaaacatcg acaaacgacg cgcgggtaaa
     2221 gcgcgccatc acggctgcca ctgccgcgcc gagcgtcagg gaggataaaa tgtagtgctg
     2281 ccaactgtct gctcccacgg taggcagcca gcccagttca acggagaata cctgaattaa
     2341 aagcatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg tcatactcaa
     2401 tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca taccaaatat
     2461 caccgcccag accatactgg ttatggtcag ccacagcgtt ggcataaagc ggctggcaat
     2521 ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct gcacagcatt
     2581 gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct gctgacgcac
     2641 cagttctata acctgcgcat cagcttcggg cccggcaatc aatcgcgccg gatcgccggg
     2701 cagcatatgc acaaataaaa acaccagcac cgagacgata aacagcgtcg gaatcaaccc
     2761 cagtaagcgt ttgataacgt aattaagcat tccactccct gcgttgctta ttgcaaatcc
     2821 gcgtcttcaa agctgaagcc ggtgtctggc atgatccaaa aaccggtcag gtttttactg
     2881 tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgactcttg ccagatgata
     2941 tcctgcgccg ccttatacaa gcgggtcttt tccgccggat cattagtttt cagtgcctga
     3001 gccaggaagt cgtccacctg tttattgctg taaaatgcgg tattaaacag cgtcggtggc
     3061 cagttttgcg aggcgaacag tggcgatagc gcccagtccg cttcgccggt tgaagccgac
     3121 cagccagtgt agaacatccg cacgccgctc tctttttgcc ctttaccttc aacttctgcc
     3181 gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg cgctaactgc
     3241 tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg
     3301 gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg
     3361 taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac accagtagct
     3421 ggcgttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat ggcgtaattc
     3481 agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat actgatataa
     3541 cgctgcataa ttgactgact ggccatcaac tcgatatttt tgtttttctc cagcagtgcg
     3601 gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg
     3661 cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg caatcctggc
     3721 tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca ggtatccagt
     3781 tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt ttccagcgct
     3841 gccggtgaaa tcatcgcggt cgccggatgg acaagaatat taataaacgc tgagaacggc
     3901 tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc aatattctta
     3961 tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag attcgctttc
     4021 accgccgcgg cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg caatttcacg
     4081 gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt tttcagtttc
     4141 gtctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc ctgagataac
     4201 gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc caccaccaca
     4261 tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag cgccactaac
     4321 ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc tacgcatgaa
     4381 tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt gcaacgagac
     4441 ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg acagcagtac
     4501 acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta atttacgcgt
     4561 ataaggatgc tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa caatttgccc
     4621 gagatacatc accgccacac gatgactaat ccgctctacc acggccatat cgtgggagat
     4681 aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga taatctgccc
     4741 gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt ttggattcaa
     4801 tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact catgcgggta
     4861 acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc
     4921 cgcgtcttta cctggcaata aaccgtgtac acacagcggt tcgataatcg aatcaccgat
     4981 ggtctgacgt gggtccagtg aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg
     5041 caatgcctga agtttgccag gtgacaaggt atcgattcgc tgaccgttaa agataatttc
     5101 gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc
     5161 agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga ctttctcaac
     5221 ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac
     5281 aagattacgc actcgtaaaa caggttcgcc atccaccacc gttttctgct cgatgggggg
     5341 ttcctgtttc gctggatgtt caagcgatat caatgggaaa cgtcggggat aatctaaccc
     5401 tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag gatgttgcgg
     5461 tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat acatcaccag
     5521 tacccgatcg gctatctctg ccaccacgcc catatcgtgc gtgataaaga taacgcccat
     5581 cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc
     5641 cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca gcgccatcgc
     5701 aatcatcact cgctggcgca tcccgccaga gagttgatgc ggataacgtg aaagaatggt
     5761 ttgtgcctca ggaatgcgta cctgatccag catccgcttc gcctcgacca tcgcttcttc
     5821 acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca cagtaaatac
     5881 cgggttcagc gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac cgcgaacatg
     5941 gcgcatttgt gcagcgctct gctcgctaag ttcaatcact tcgcgactgc gccgctgcaa
     6001 cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca tcaatgccaa
     6061 cgcagtcact gacttaccgg agccggattc gccaacaatt gccagcgtct caccgcgttg
     6121 cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca taaaggcaat
     6181 attcagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt gtggcaacgg
     6241 tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc
     6301 gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggccg atagcgatta
     6361 agccaccgct accgccaagc gcagggagtt tttccattac tacccgctcg caggcttccg
     6421 cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc agcgcgcgga
     6481 tgaagacttc gcccgtgccg gtacaagaaa ccgccacact ggcgttattg gcgtagcatc
     6541 cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc attccgcctg
     6601 tggacgtggc tgccgccaaa ttgccgtcta aatccaacgc cacggccccc acggtgccca
     6661 ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc ccttccttgc
     6721 gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatcgccggc gagacgcgct
     6781 ccatgccacg agcaaacgca aaattttctg ccccttcgcc aatcatcatc acatgcgggc
     6841 tttgctccat caccagccgg gcggcaagaa ccggattacg cagatgacta acgcccgcca
     6901 ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca tgggtttcat
     6961 cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc agcagacgca
     7021 ccgcttccgt caccacatcc agcgcacttt cgcccgcttc cagcattttc tgcccggttt
     7081 caacaatggc agacaacgcc tcaatgtagc gtaattcctg ttgcagactc atctgcgcgc
     7141 ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata gtattcgtcc
     7201 attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat tactcattca
     7261 atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg ctacgcccga
     7321 ggtggcatac agtctgtggc gtttttctga cataataggc aaattcgatt ttgcctccgc
     7381 aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag
     7441 atgctttctc gcgtcacccc actgaccgcc caggaaacgc tgccactggt acagtgtttt
     7501 ggtcgtattc tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt tgataactcc
     7561 gcaatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt
     7621 gccggtaaat cctttgccgg tcagccgtat catggtgaat ggcctgcggg cacctgcatt
     7681 cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag
     7741 actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt
     7801 cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt tcccggcggg aactcgcctg
     7861 actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc ggtgattcgt
     7921 aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg tcagccgctg
     7981 ggtgacggcc aaatctacga caccaaccat ctcgccgtac acctgatgtt agaacagctg
     8041 ggttgcgagg taatcaactt agggattatc cgcgacgatc cccatgccct gcgcgccgca
     8101 tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt
     8161 gaagcggatt acaccaaaac gattcttgaa gagctggggg agatcgcctt ctggaagctg
     8221 gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg gttctgcggc
     8281 ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca gcctttgctg
     8341 gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg
     8401 tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct gcaacgcaac
     8461 gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat atttagctcc
     8521 tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc
     8581 gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg gcggaactca
     8641 gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt gattttgacg
     8701 gtcaggaggc gctaaaagat tctcgtgtgc tgatcgtcgg cctcggtgga ctcggctgcg
     8761 cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc gacttcgaca
     8821 cggtttcgct ctcgaatctg caacgccaga cactgcacaa tgatgccacg gtcgggcaac
     8881 cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc gcgattacgc
     8941 cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa tacgatctgg
     9001 tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc tgttttgccg
     9061 cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca
     9121 cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc gagaacgcgt
     9181 taacctgcgt agaagcaggc gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg
     9241 caatggaagc gatcaaactg ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca
     9301 tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgtaat ccggggtgtg
     9361 aggtgtgcgg gcagtaattg ccacctccag gccagataag acgcggcact ccgccacgtc
     9421 atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc
     9481 agccagtcct gttcaaactt cgccacagcg gcatcaaccg ccggatagct aatcatctgt
     9541 tgtgccacat ccagtggcag cgtaattgat tcacatcctg ccagtaagca gtccagcgcc
     9601 tgacgcgggt ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat
     9661 aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta
     9721 acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcagcccttg tgctgcgcca
     9781 tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat agctgccagc
     9841 ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg caatttacgt
     9901 gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga
     9961 ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg
    10021 ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcact
    10081 gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg
    10141 tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatccaactt
    10201 tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt
    10261 cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt
    10321 tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg
    10381 tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggcagc gccggaagtg
    10441 attgagcgcg cattgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg
    10501 acggcgctaa ccgactgctg cccgacacag gcgttaaccg tgtgtgggga agtgaaaagc
    10561 gttgaggaga tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc
    10621 ggcttaacgc tttcgggtgg tgaacccttt atgcagccgg aaatagcgac ggcgctactg
    10681 caagcctgcc acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg
    10741 aaatatatca ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacatt
    10801 gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccaaggtgct ggataacctg
    10861 aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt
    10921 aatgccgatg aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt
    10981 ggcgaaattc attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat
    11041 ctgccctatg acgccccgga aaaaccgctt gatgcgccag acctgctcga ctttgcccag
    11101 cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac
    11161 tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg
    11221 tgaaaccgcc ggtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc
    11281 tcgataagcc gatcccggta cgtcgcgcgc tggctctggc gcatcacctg gcgaatcgca
    11341 ccatctggat caagcacgat gagttgatca tcggcaacca ggcaagcgag gttcgcgctg
    11401 cgccgatctt cccggaatat accgtctcgt ggatcgaaaa agagattgat gatctggcag
    11461 atcgtcccgg tgctggtttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt
    11521 gcccgtggtg gcgcggtcag acggtacagg atcgctgcta cggcatgttt accgatgagc
    11581 aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc tccggcgatg
    11641 cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt ctgcgcgaga
    11701 aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg cacggcgaac
    11761 agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt gaacgttttg
    11821 cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc
    11881 tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg
    11941 cgctgcaact gtgttacttc atccagttga tattgcagat cgaatctaac ggtcactcgg
    12001 tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgagac gttgaactca
    12061 accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ctgaaactgc
    12121 tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga agtcctctgt
    12181 atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg gacgcggtga
    12241 atccactctc ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc
    12301 tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc gtacaggtga
    12361 tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta
    12421 ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt tgtatcgaaa
    12481 ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc
    12541 gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac aagcggcaac gtgttcctgc
    12601 cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta atggacgcgt
    12661 gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca
    12721 ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg
    12781 agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg
    12841 ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta tttgaacagg
    12901 gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc
    12961 atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata
    13021 ctgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc
    13081 ataatccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcatcta
    13141 tctccgctaa cgtaccgttt ggtgcgcaaa ctatggcaac gccggacggg cgtaaagccc
    13201 acaccccgct ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg
    13261 cggtcattgg ctcggtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca
    13321 accagaaact gaatccggca acgctggaga acgaatctga caagcagaag ctgatgatcc
    13381 tgctgcgcac cttcttcgag gtgcataaag gctggcatat tcagtacaac atcgtttccc
    13441 gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg
    13501 tcgcgggtta ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg
    13561 ctcgtactga acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc
    13621 attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac
    13681 attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa
    13741 gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca
    13801 cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt tgtcgccagc
    13861 ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt
    13921 gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg
    13981 acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt
    14041 gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca
    14101 ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga gattgacgac
    14161 gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt gatcgacaaa
    14221 ctgcacatag cgttcgatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac
    14281 ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat
    14341 ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa
    14401 atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt
    14461 tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat
    14521 aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg gttttccctt
    14581 ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt
    14641 atattgtcac atgcaaatca taatggtttc gaatgaaagt taatgagagg tggttatggc
    14701 ttctactttt accagcgaca cattgccagc cgatcacaaa gcagctatcc gtcagatgaa
    14761 gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc taagcgatga
    14821 cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc
    14881 ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat
    14941 taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg
    15001 ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc
    15061 cggcgataat ttcttcggga cgctcagcac ttcacgtccc gagatttacc ccatctactg
    15121 gtcgcagacg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct
    15181 caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt
    15241 catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag gtcttggagc
    15301 gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc gcgttttcgc
    15361 caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac atcgtacgga
    15421 agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg
    15481 ctggatagcg ctctctgata tgctgcctgg tcaggggttg ctgcacgttg tgcccattcc
    15541 tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact
    15601 gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat
    15661 tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga
    15721 cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat
    15781 tcctgcggca ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact
    15841 ggaaaaaggc gcatcgccgg gcgacttccc gcacgaggac tatgaaacaa actgggaagg
    15901 acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg
    15961 agagttaatg gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt
    16021 gctgacaagt gcgctttctt aatgcctgat gcggcgttaa cgccttatct ggcctacaaa
    16081 gtcctgcaga ttcaatatat tacaggtact gcgtaggcct gataagcgt
//
LOCUS       DADLTD010000090        16104 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLTD010000090 DADLTD010000000
VERSION     DADLTD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.090"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..224)
                     /locus_tag="JN701_002239"
     CDS             complement(<1..224)
                     /locus_tag="JN701_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HAY7364683.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLR"
     gene            complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="JN701_002240"
     CDS             complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="JN701_002240"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY7364684.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="JN701_002241"
     CDS             complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="JN701_002241"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY7364685.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="JN701_002242"
     CDS             complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="JN701_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY7364686.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4373..5671)
                     /gene="purA"
                     /locus_tag="JN701_002243"
     CDS             complement(4373..5671)
                     /gene="purA"
                     /locus_tag="JN701_002243"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY7364687.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIISNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5775..5972)
                     /locus_tag="JN701_002244"
     CDS             complement(5775..5972)
                     /locus_tag="JN701_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY7364688.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="JN701_002245"
     CDS             complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="JN701_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY7364689.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="JN701_002246"
     CDS             complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="JN701_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY7364690.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="JN701_002247"
     CDS             complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="JN701_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY7364691.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="JN701_002248"
     CDS             complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="JN701_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY7364692.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="JN701_002249"
     CDS             complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="JN701_002249"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY7364693.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="JN701_002250"
     CDS             complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="JN701_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY7364694.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="JN701_002251"
     CDS             complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="JN701_002251"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY7364695.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="JN701_002252"
     CDS             complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="JN701_002252"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY7364696.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14746..>16104)
                     /gene="nnr"
                     /locus_tag="JN701_002253"
     CDS             complement(14746..>16104)
                     /gene="nnr"
                     /locus_tag="JN701_002253"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7364697.1"
                     /translation="LVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLPEEAAL
                     AREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSHPAPIV
                     AVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLGLDSWL
                     AGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAG
                     AGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQEWGKKA
                     LQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIESDRLHC
                     AKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGALLGQK
                     LSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHDESSNS
                     AP"
BASE COUNT         3661 a         4618 c         3981 g         3844 t
ORIGIN      
        1 tttcttaata agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata
       61 aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa
      121 tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt
      181 acagtttgca gcgccgtcgc cagcgccaac agattccatg tcatatatta ttccatatag
      241 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
      301 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
      361 tgacgcaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag agaagaaaca
      421 cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata
      481 ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt
      541 gtatgatccg cctcacctgc cgtaccgacg atccagatat tctcttcctg caacatacgc
      601 atggtacgca ccaggttagt cacccgaatc agcggaacgc tttctgccgc gccacaggcc
      661 acttttttcg ccgtggcgtt gagctgggcg gagcgatctt tcggcacaat caccgcatga
      721 acaccagcgg catctgcgct acgcaggcac gcgccgaggt tatgcggatc ggttacaccg
      781 tcaaggatca gcaggaacgg ctgatcgagc gaagcgatca gatccggcaa atcattttcc
      841 tggtactgac gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt
      901 ttctcgtcga gatattggcg gtttgccagc tggataacca cgccctgggc ttcgagggcg
      961 tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa
     1021 cgttcagggg cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc
     1081 attaatgtac tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat
     1141 ctctgcaata tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc
     1201 cggcatgaac aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga agagggtaat
     1261 gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc
     1321 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc
     1381 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgaccaga
     1441 cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1501 ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc
     1561 atattaaccg cttcgacgcg aacttccacg cgatcgccca gacgataagt ctggccgctg
     1621 gattcaccca tcagccgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1681 gagacatgga ccagaccatc aatgaataag tcgtccagac ggacaaagaa tccaaagcca
     1741 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1801 ttcagccagt cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1861 tgctgaccca gttgcagcat ttcttccatc gaataatggt agccgccggt ttcagtggtg
     1921 ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg
     1981 tctggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     2041 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     2101 agcatttctg catcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt
     2161 tccggcttgt taccgcccgg cagttccagc cccagctccg ccagtactga acggaaagag
     2221 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca atgctggttc tttggctttc
     2281 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt
     2341 gcgtcgttac gctgagtctg ttcgatacgc tcaatacggc attcagcgtt gaaaataaac
     2401 ttcgcttctt cgctctcaaa tgagatcccg ccacgttctt cacgggcttt atccagcact
     2461 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gagcgtactg ctcgcgcaga
     2521 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2581 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2641 cacaccatac acaggcggtc tacctgcgga ttgagcgagc acagaccgtt agagagcact
     2701 tccggcagca tagggataac ctgcgaaggg aagtacaccg acgtaccacg attacgcgct
     2761 tctctatcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2821 aaacgccagc cgccgccgcg ttttttctcg cagtaaacag catcgtcaaa gtcacgggcg
     2881 tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct
     2941 tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg
     3001 tacggaattt catgggtacg caaagcgata tcaaccgcca tgccggtgcc catattgtcg
     3061 cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt
     3121 tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     3181 tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     3241 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     3301 cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat gcaggttttc
     3361 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3421 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3481 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3541 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3601 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3661 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3721 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3781 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3841 tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3901 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3961 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     4021 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     4081 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     4141 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     4201 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     4261 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca
     4321 atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     4381 cgaacgggtc gcgcagaatc atagtttcag tacgatccgg accggtagag atgatatcga
     4441 tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcagcc
     4501 cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4561 gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg
     4621 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag
     4681 tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4741 gccagccggt acgacgacga cgacccgtcg ttgcgccgaa ttcgttaccc tgcttgcaga
     4801 ggaactcgcc agtttcatca aacagttcgg tcgggaaagg acctgcaccc acacgagtgg
     4861 agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg
     4921 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4981 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     5041 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     5101 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     5161 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     5221 cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac
     5281 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     5341 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     5401 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5461 tactgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5521 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5581 catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5641 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5701 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5761 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5821 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5881 atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc
     5941 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     6001 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     6061 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     6121 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     6181 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     6241 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     6301 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     6361 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6421 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6481 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6541 ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6601 tcacgtactt cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg
     6661 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6721 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6781 atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6841 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6901 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6961 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     7021 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     7081 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     7141 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcaaacgc agcagattgc
     7201 tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg
     7261 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     7321 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     7381 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7441 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7501 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7561 catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca
     7621 gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgggtatcgc
     7681 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataaccc
     7741 cgcgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7801 gatatttttc cggataggta acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7861 cgtccgacgt cagcatcaca ccggaagcgg ccagttcacg cacggcttcc acgtttaccg
     7921 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7981 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     8041 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgtg
     8101 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     8161 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     8221 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     8281 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     8341 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     8401 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8461 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8521 attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat
     8581 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8641 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8701 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8761 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc
     8821 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8881 gctttaaatg ccgccaccag atcgtgcggc agatggcgaa taaaccctac ggtatctgca
     8941 agtacggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca
     9001 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     9061 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     9121 cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga
     9181 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     9241 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     9301 agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     9361 tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc caggttacgc
     9421 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9481 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9541 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagaaaccag agattcaaac
     9601 tcctggaggt cttccatatc tttgtctttc gtaaaataga tgtgtaccag taccgcctgc
     9661 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9721 caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9781 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9841 gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag tagaaatcgc
     9901 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9961 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    10021 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    10081 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    10141 tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg
    10201 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    10261 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    10321 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    10381 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    10441 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    10501 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    10561 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    10621 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    10681 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10741 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10801 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10861 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10921 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    10981 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    11041 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    11101 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    11161 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    11221 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgagcaata
    11281 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    11341 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    11401 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    11461 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca aactggcgct
    11521 tcacccgggg tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt
    11581 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    11641 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    11701 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11761 cctggctgcg cattcggcca aggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    11821 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11881 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    11941 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    12001 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    12061 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    12121 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    12181 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    12241 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    12301 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    12361 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    12421 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    12481 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    12541 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    12601 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    12661 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12721 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12781 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12841 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12901 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12961 tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc
    13021 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    13081 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    13141 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    13201 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    13261 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    13321 aaatagggat cagactgact gttcgccagc acatcgcccg ccccacccag cagctccgaa
    13381 tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc
    13441 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    13501 aggaaattag cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga aataaagtaa
    13561 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat
    13621 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    13681 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13741 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13801 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13861 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    13921 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    13981 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    14041 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    14101 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    14161 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    14221 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    14281 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    14341 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    14401 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    14461 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    14521 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    14581 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaagccccgg
    14641 ctaaaggtgg tcttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    14701 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14761 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14821 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14881 cgcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt tttgcccaag
    14941 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    15001 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    15061 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    15121 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    15181 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    15241 caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc actcttgctg
    15301 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    15361 catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc
    15421 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    15481 acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc cgtgatcgcc
    15541 tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg ctgaaaaccg
    15601 ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt caaaatgcag
    15661 ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    15721 gatggtgtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag ccagcaggcc
    15781 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15841 gctaatggat tcgcggggcg cttgccgcaa accggtaccg agcagcgcat caacaatcag
    15901 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15961 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggcaaacaa
    16021 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    16081 attaccatga ccgcacagca ccag
//
LOCUS       DADLTD010000091        15701 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLTD010000091 DADLTD010000000
VERSION     DADLTD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.091"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..881)
                     /locus_tag="JN701_002254"
     CDS             complement(<1..881)
                     /locus_tag="JN701_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7364698.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYY"
     gene            1405..2937
                     /gene="pntA"
                     /locus_tag="JN701_002255"
     CDS             1405..2937
                     /gene="pntA"
                     /locus_tag="JN701_002255"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY7364699.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            2948..4336
                     /gene="pntB"
                     /locus_tag="JN701_002256"
     CDS             2948..4336
                     /gene="pntB"
                     /locus_tag="JN701_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY7364700.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(4361..5395)
                     /gene="tqsA"
                     /locus_tag="JN701_002257"
     CDS             complement(4361..5395)
                     /gene="tqsA"
                     /locus_tag="JN701_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7364701.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            5807..6172
                     /gene="mdtJ"
                     /locus_tag="JN701_002258"
     CDS             5807..6172
                     /gene="mdtJ"
                     /locus_tag="JN701_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY7364702.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            6159..6488
                     /gene="mdtI"
                     /locus_tag="JN701_002259"
     CDS             6159..6488
                     /gene="mdtI"
                     /locus_tag="JN701_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY7364703.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(6527..7348)
                     /locus_tag="JN701_002260"
     CDS             complement(6527..7348)
                     /locus_tag="JN701_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY7364704.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTATLKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(7448..7531)
                     /locus_tag="JN701_002261"
     CDS             complement(7448..7531)
                     /locus_tag="JN701_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364705.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(7624..7938)
                     /locus_tag="JN701_002262"
     CDS             complement(7624..7938)
                     /locus_tag="JN701_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY7364706.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            complement(8335..9587)
                     /locus_tag="JN701_002263"
                     /pseudo
     CDS             complement(8335..9587)
                     /locus_tag="JN701_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9694..10587
                     /locus_tag="JN701_002264"
     CDS             9694..10587
                     /locus_tag="JN701_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7364707.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            10722..11942
                     /gene="mlc"
                     /locus_tag="JN701_002265"
                     /gene_synonym="dgsA"
     CDS             10722..11942
                     /gene="mlc"
                     /locus_tag="JN701_002265"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY7364708.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            12067..12762
                     /gene="bioD"
                     /locus_tag="JN701_002266"
     CDS             12067..12762
                     /gene="bioD"
                     /locus_tag="JN701_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7364709.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(12715..14007)
                     /gene="clcB"
                     /locus_tag="JN701_002267"
     CDS             complement(12715..14007)
                     /gene="clcB"
                     /locus_tag="JN701_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY7364710.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(14166..14768)
                     /gene="dmsD"
                     /locus_tag="JN701_002268"
     CDS             complement(14166..14768)
                     /gene="dmsD"
                     /locus_tag="JN701_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY7364711.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPPLFR"
     gene            complement(14811..15665)
                     /locus_tag="JN701_002269"
     CDS             complement(14811..15665)
                     /locus_tag="JN701_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7364712.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCASLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
BASE COUNT         4012 a         3862 c         4062 g         3765 t
ORIGIN      
        1 tggtaatact tagcaccttt cttcgcggca cgtttcgcaa cctgatagga gacttcggtc
       61 atgttgccgt agttgccaga gaatttgatg ctatcgaacg ggaccatcat cgctgcggtc
      121 gctttgttca gttcttcgac tttagtgcca tccgggagcg tgacggtgta acgcccgcct
      181 tttgatgact gggtttcaaa gaaacgaccg acttcagaac ttggtgatgc ggtagtcgca
      241 acacctggga tctcaacttt cttcgcggct tcgccaccgg cagccagagc tgcacgtcct
      301 gcttcggaat ctgccgggat cacatccggg ctctggacga tacgtttctt agcatctttt
      361 ttatagatga atgcagtaat acgctggttg ccgccctggt tggcatcgat ttgacgaacg
      421 atgtagaaag agtaggcacc tttcgctttt gccgctttgg tgatggcatc attgacttct
      481 ggctggctac gatagaagcc ctggacggtg accgtgtcaa acggttcaat cagaacagcc
      541 tgatctttcg gcagttcgac aacaccgtta attacgcgat tacttgtttc ttctgctttt
      601 tcagcatcgg ctttatagag gtcagcgacc acacgccagt taccgctgtt accaaaatca
      661 gaagtgtcga caacataaaa agaggcggca ccttctttat ctgcgcgacg agaaacggct
      721 ttcaccgctt cgccaatagc attaaaacga ccggtaacca ctacacggtc aaaaggttta
      781 accgctgccg cttgctccgg tgtcagttct gttgctgcgt taacggagaa tgccgtagca
      841 gaaagcagtg ccgacgccag gagggtgttc ttaagcttca taaaaataat ccttcgcctt
      901 gcgcaaacca ggtactggta ttgttattaa cgagaaacgt ggctgattat tgcatttaaa
      961 cggtgtaact gtctgcgtca tttttcatat cacattcctt aagccaattt taatcctgct
     1021 caaatgaccg tctatgctta aaaaacagcc gtatcagcat cattactact gaagcaactg
     1081 aattgtataa gttaatttaa tgttaagtag tgaatcgtgc cggggcgatg tctcgtttta
     1141 cccgaccgtc gaagacaatt atcagtcttt atccggcgtt ctaaggtgtt tatcccacta
     1201 tcacggctga atcgttaata ttttgcgagt tcacgccgaa atactgattt ttggcgctag
     1261 atcacaggta taattttcag tacgttatag ggcgtttgtt actaatttat tttaacggag
     1321 taacatttag ctcgtacatg agcggcttgt gtggctcctg acacaggcaa accatcatca
     1381 ataaaaccga tggaagggaa tatcatgcga attggcatac caagagaacg gttaaccaat
     1441 gaaacccgtg ttgcagcaac gccaaaaaca gtggaacagc tgctgaaact gggttttacc
     1501 gtcgcggtag agagcggcgc gggtcaactg gcaagttttg acgataaagc gtttgtgcaa
     1561 gcgggcgctg aaattgtaga agggaatagc gtctggcagt cagagatcat tctgaaggtc
     1621 aatgcgccgt tagatgatga aattgcgtta ctgaatcctg ggacaacgct ggtgagtttt
     1681 atctggcctg cgcagaatcc ggaattaatg caaaaacttg cggaacgtaa cgtgaccgtg
     1741 atggcgatgg attctgtgcc gcgtatctca cgcgcacaat cgctggacgc actaagctcg
     1801 atggcgaaca tcgccggtta tcgcgccatt gttgaagcgg cacatgaatt tgggcgcttc
     1861 tttaccgggc aaattactgc ggccgggaaa gtgccaccgg caaaagtgat ggtgattggt
     1921 gcgggtgttg caggtctggc cgccattggc gcagcaaaca gtctcggcgc gattgtgcgt
     1981 gcattcgaca cccgtccgga agtgaaagaa caagttcaaa gtatgggcgc ggaattcctc
     2041 gagctggatt ttaaagagga agcgggcagc ggcgatggct atgccaaagt gatgtcggac
     2101 gcgttcatta aagcagaaat ggaactcttt gccgcccagg caaaagaggt cgatatcatt
     2161 gtcaccaccg cgcttattcc aggcaaacca gcgccgaagc taattacccg tgaaatggtt
     2221 gactccatga agacgggcag tgtgattgtc gacctggcag cccaaaacgg tggcaactgt
     2281 gaatacaccg tgccgggtga aatcttcact acggaaaatg gtgtcaaagt gattggttat
     2341 accgatcttc cgggccgtct gccgacgcaa tcctcgcagc tttacggtac taacctcgtt
     2401 aatctgctga aactgttgtg caaagagaaa gacggcaata tcactgttga ttttgatgat
     2461 gtggtgattc gccgcgtgac cgtgatccgt gcgggcgaaa ttacctggcc ggcaccgccg
     2521 attcaggtat cagctcagcc gcaggcggca caaaaagcgg caccggaagt gaaaactgag
     2581 gaaaaatgtg cctgctcacc gtggcgtaaa tacgcgttga tggcgctggc aatcatcctt
     2641 ttcggttggc tggcaagcgt tgcgccgaaa gagtttcttg ggcacttcac cgttttcgcg
     2701 ctggcctgcg ttgtcggtta ttacgtggtg tggaatgtat cgcacgcgct gcatacaccg
     2761 ttgatgtcgg tcaccaacgc gatttcaggg attattgttg tcggagcact gttgcagatt
     2821 ggccagggcg gctgggttag cttccttagt tttatcgcgg tgcttatagc cagcattaat
     2881 attttcggtg gcttcaccgt gactcagcgc atgctgaaaa tgttccgcaa aaattaaggg
     2941 gtaacatatg tctggaggat tagttacagc tgcatacatt gttgccgcga tcctgtttat
     3001 cttcagtctg gccggtcttt cgaaacatga aacgtctcgc cagggtaaca acttcggtat
     3061 cgcagggatg gcgattgcgt taatcgcaac catttttgga ccggatacgg gtaatgttgg
     3121 ctggatcttg ctggcgatgg tcattggtgg ggcaattggt atccgtctgg cgaagaaagt
     3181 tgaaatgacc gaaatgccag aactggtggc gatcctgcac agcttcgtgg gtctggcggc
     3241 agtgctggtt ggctttaaca gctatctgca tcatgacgcg ggaatggcac cgattctggt
     3301 caatattcac ctgacggaag tgttcctcgg tatcttcatc ggggcggtaa cgttcacggg
     3361 ttcggtggtg gcgttcggca aactgtgtgg caagatttcg tctaaaccgt tgatgctgcc
     3421 aaaccgtcac aaaatgaacc tggcggctct ggtcgtttcc ttcctgctgc tgattgtatt
     3481 tgttcgcacg gacagcgtcg gcctgcaagt gctggcattg ctaataatga ccgccattgc
     3541 gctggtattc ggctggcatt tagtcgcctc catcggtggt gcagatatgc cagtggtggt
     3601 gtcgatgctg aactcgtact ccggctgggc ggctgcggct gcgggcttta tgctcagcaa
     3661 cgacctgctg attgtgaccg gtgcgctggt cggttcttcg ggggctatcc tttcttacat
     3721 tatgtgtaag gcgatgaacc gttcctttat cagcgttatt gcgggtggtt tcggcaccga
     3781 cggctcttct actggcgatg atcaggaagt gggcgagcac cgcgaaatca ccgcagaaga
     3841 gacagcggaa ctgctgaaaa actcccattc agtgatcatt actccggggt acggcatggc
     3901 agtcgcgcag gcgcaatatc ctgtcgctga aattaccgag aaactgcgcg ctcgtggtat
     3961 caacgtgcgt ttcggtatcc acccggttgc ggggcgtttg cctggacata tgaacgtatt
     4021 gctggctgaa gcaaaagtac cgtatgacat cgtgctggaa atggacgaga tcaacgatga
     4081 ctttgctgat accgataccg tactggtgat tggtgctaac gatacggtta acccggcggc
     4141 gcaggatgat ccgaagagtc ctattgctgg tatgcctgtg ctggaagtgt ggaaagcgca
     4201 ggacgtgatt gtctttaaac gttcgatgaa cactggctat gctggtgtgc aaaacccgct
     4261 gttcttcaag gaaaacaccc acatgctgtt tggtgacgcc aaagccagcg tggatgcaat
     4321 cctgaaagct ctgtaaccct gacggcctct gcggaggccg tcactcttta ttgagatcgc
     4381 ttaacagaac ggcgatgctt tgacctcccg ctgtttgttc aagcgcaatt ttgacaataa
     4441 ttgttaacgg cacggaaagc agcataccca ccggtcctaa caaccatccc caaaaaatca
     4501 acgacaaaaa taccaccaat gtggaaagcc ccagcccacg tcccatgatg cgcggctcca
     4561 gaatattgcc gaagaccaga ttaatcagca gatatcccgc cagcaccagc aacgcttcgt
     4621 agaagccatt aaacaccagt acctgagcga taggggggat tgccgcgaga actgaaccaa
     4681 tattagggat gtaattaagc gcaaaggcca gcaatcccca gacaaaagcg aagcgaacat
     4741 cgagtgcggc gagcatcgcc caggcgacca ggccggtgat gatgctgatg gctgttttca
     4801 gcaccagata atgagaaaca ctgtcaattg cacgttgaat cgccgccatc ccttcaaccg
     4861 gacgcgccat catttgctga aattttccgg gcaattgtgg cacttcgagc agcataaaca
     4921 gcaccgtcag cagcaataaa aatattgatg acatggcatt agataactgc gtcaataagt
     4981 tggtgagcaa cgtcatcgct gcgttcggat caatgtaatg cgccagctgg tcaactgaga
     5041 cgtcaatccc tacgcgttgc aacaacggct caagagcttg cagcggcgtc ataatagagt
     5101 tgcgatattg cggtaacgtc cgcgtcaact cgttgagcgc ggaacccaga taagctaata
     5161 gcaacaccat cgccatcacg atgatggtca tcaaaatcga caccgccagt acacgcggca
     5221 ctcgccagcg gaccatgtgt tgcaccagcg ggttaagaat aacagcaata aataatgcga
     5281 gaataaacgg cacgatgatc tcggcggcaa aacggatacc gcagagaata atgaccagca
     5341 ttcccaacat aatgacgatt tttaggccat tgagcgtgat gatcggcttt gccatgcttg
     5401 tccccgtttt ttcttccttt gctaataata atgtcttttt cgctccgtca tcataacgaa
     5461 aatcaaaccg ctcaggtaaa gaagtgaaaa tattttgagt taattcttaa gctatgatac
     5521 aaatcaggcg tgttcaacta ccgaggacaa ttatcatccg cgatgacgag aagcaacact
     5581 gcggataatt gtaatattat ggacaatttg ttcaggacct tattttcaac atttacccat
     5641 ctttgtacct catccactat cttgctcgta ggtgagcagc actggcgcaa cgcgctttag
     5701 tcctttcttc tgcctaagct tgccctatgc gcggggcttg accactttac tttgatttcg
     5761 ctgaattaag cgaaaattaa aataattctc ttgcaggaga aggacaatgt atatttattg
     5821 gattttatta ggcctggcta ttgctacaga aattaccggt acgctgtcaa tgaaatgggc
     5881 gagcgtcagt gagggaaatg gcggctttat tttaatgctg gtgatgattt ctctgtcgta
     5941 tatatttctc tctttcgccg ttaaaaagat cgccttaggc gtagcttatg cgctgtggga
     6001 aggtatcggt attttattta ttaccttgtt tagcgttttg ttattcgacg aaagtttatc
     6061 gctgatgaaa attgccgggt taaccaccct ggtcgccggg attgtgttga taaaatcagg
     6121 tacccgtaaa gcgcgtaaac ctgaactgga ggtaaaccat ggcgcagttt gaatgggttc
     6181 acgccgcctg gctggcattg gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat
     6241 tttctgacgg ctttcgtcgc aaaatatttg gcttgctctc cctggcggcg gtgctggctg
     6301 cctttagtgc gctttctcag gccgttaaag ggatcgactt gtctgtcgct tatgcattgt
     6361 ggggcgggtt tggtattgcc gccacgttag ccgcaggttg gatcttgttt ggtcaacggt
     6421 taaatcgtaa aggctggatt ggcctggtct tgctgttggc tggaatgatc atggtgaaac
     6481 ttgcctgatg aacacgctgc ccgcacagtc gggcagcgtt tgaacattat ttttgcgaca
     6541 gttgatccag cttgtcgcga aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc
     6601 gatctttcgc ggcaggagcc gaactttgca ccccaattaa ttgccagccg tcattggtat
     6661 gcaacatcag aggcgaaccg ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg
     6721 acgtttgcgc ccagccagtc acttcacagt tttgatgact gtacaacgta tcgagatgat
     6781 cttcagggta tcccgcctga gttactttac ggccagccga ttttaatgtg gcagtaagcg
     6841 cggctttatc tccctcaaat aacggcaacg gtgtaatgcc agaaggggga ttacgtagca
     6901 caatcaatcc gaagtcccac ggcgcggctg cgggaggtac aatccaacca tccccatctg
     6961 cttttaaccg ctttcccagt gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc
     7021 gccaaagacc tttatttgac acaaaacgca gcaccactgc tttatcggct ttaccctttg
     7081 gaggtgtcaa taaacagtgt cctgccgtta atgccagatt gggtgcaatc agcgtcgccg
     7141 tacataaatt gccgctggcc gtttccagtt gcccaaccgc atcccacggt gattgggccg
     7201 tgtcattcac tggcacacga tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg
     7261 ccgatttggc aacgtctggt ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac
     7321 ccaacactac agcaatggtt gtacgcatat cacactctgg tgggtaatta tgattattaa
     7381 aagcaaaccc tcataaatac tatagacggg acgggatgaa agtgggagta aaatcagata
     7441 actacagtca ggaaagataa aaacgggcgg caatcagtgc gcataaaata aggatgataa
     7501 gaatgaactc aaagcgataa cggcccacca tactaccctc cgctaaaggc ggtgctaaac
     7561 gttacccgct tagcaccgta attttcaggc gcgagggggc gcgccagcat tactgttgaa
     7621 aacttacgct gcgggttgtg cagcaggttt tgccggttgc tgatgactgt gtttcccggc
     7681 gtgtttcttc gctgcctgcg ctttttgttc aggggctttc tgttcagctt tcgtattttt
     7741 atgatgcttt ttagctgcct gcgctttctg ggcaggtgct gctttatgct gttttttatg
     7801 atgtgtcgtt ttcgccggcg ctgctttggt ggtcgtcgca gtctctgcag caaaggcggc
     7861 agaagacaga cccatagcag cggcaacaac cagagctaat acttttttca ttgtcatacc
     7921 ctcaatttgt tttttcattt aaccccactg cggggccgtt gaaataacta tatccctggg
     7981 aatggcagac ttccgtgagt ggttggtttc agcgtgtaac gatatgtaca aacgctgaat
     8041 aaattacagc attgataata acgtattgtg acttaaggga aatttagcta ccaataatag
     8101 tagtcttgat cgggttaact attttactac ttacaaagcg gtagaataac tgcgcatcag
     8161 taataaatga cacacagcaa aatgaatccg tttatttggg tacttataat cctgatgacg
     8221 ctagacgcac tgcgggaatt ggctggcgct tcgtctattt taggatggct attaacgttg
     8281 gtttgagcaa gcaataagtc cggacgtgta gttaccgcag tccgggctta tttttcaggc
     8341 gtgcagacga cgatgcaaac gcgtcccgac cagcagagca atgaccagca tcagagcaat
     8401 aaatgcgccg acgccgttcc agccatagtt atgccagaaa acatcaccca gcgtcccggc
     8461 aatactcgac cccagatagt aactgaacag atacagcgag gaggcctggc ctttagcgcg
     8521 ttttgcgcgg gggccgatcc agctgctggc aactgagtgg gctgcgaaga atcctgctga
     8581 gaagagtaac attccggcaa agatcagcca cagcgagctg aataaggtca tcagtaaacc
     8641 aaacagcata acccccgtcg aaaacaacat caccggaccg cgcccgtagc gggtggtcat
     8701 ggttccggct ttgggtgagc tccgtgtacc ggtcaaataa gccagcgata ataagccaac
     8761 cacggcctga ctgacatgcc agggggaaag catcaaccga tagccgatgt aattaaacag
     8821 cgtgacgaac gaccccatca gcaaaaagcc ttctgcgaac aataacggta atccccggtc
     8881 acgccagtgc agacgaaagt tgataaacaa cgtcttaggt cgcagcgaag tcgggcgaaa
     8941 atggcgtgat tccggaagga ttttccagaa catcaacgcc gaggctagcg cgaaacaacc
     9001 gattgccgcc agagcaattc gccagttgaa aaagtccgtg aagacaccac taattaagcg
     9061 tccgctcatg ccgccaattg agttgccgct gatatacaac cccatcgaaa aggccacgaa
     9121 actggggtgg atttcctcgc taagataagt catgccaaca gctgccacgc cacttaacga
     9181 aagcccaatc aaggcgcgca taatcaaaat gccgtgccag ctggtcatca ttgtcgaaat
     9241 aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca ctggtttgcg accaatggca
     9301 tcggataggg ggccagtaaa cagcaaacca atagccaaca tcgccgtgga aatggacagt
     9361 gaaatactac tgttcgcggg ggttaagcca aactcctgcg aaagcaccgg aaggataggc
     9421 tgcacacaat agaggagtgc aaaggttgcc agtccggcag agaacagcgc cagggtgacg
     9481 cgcataaatt gcggcgtacc gcgtttaata aattgatttg gctgagaaat gctttgcttg
     9541 tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac ggctcacttg aaatccttgc
     9601 taaatatgcc tgtggatcag gcttatacat aggttaggaa aatcgaattg ttctgtctaa
     9661 tatattaata atctcaaata agatgtttta aatatgaata ttgaacttcg tcatctgcgt
     9721 tactttgttg ctgttgcgga agagctgcat ttcgggcgcg ccgctgcccg cctgaatatt
     9781 tcgcaaccac cgctaagtca gcagattcag gcactggagc aacaaattgg tgcccgactg
     9841 ctggcacgaa ctaatcgcag tgtattgctg acggcagcag gaaaacagtt tcttgcagat
     9901 agtcggcaaa tcctgtctat ggtagatgac gctgccgctc gcgctgaaag actgcatcag
     9961 ggtgaagcgg gggagttgcg cattggtttt acttcgtccg ctccttttat tcgggcggtg
    10021 tccgatacgt tatcgctgtt tcgccgtgat tatcccggtg tccatttaca aacccgcgaa
    10081 atgaacactc gtgagcaaat cgctccgctc attgaaggaa cgctggatat gggattgctg
    10141 cgtaacacag cgttaccgga gacgcttgaa cacgcagtca tcgtccatga accgcttatg
    10201 gcgatgatcc cgcacgatca tcccctggca aataacccga atgtaacgtt ggctgaactg
    10261 gcgaaagaac ccttcgtctt ttttgatccg cacgtcggga cagggctgta tgacgatatt
    10321 ctcgggctga tgcgacgtta ccatttgacg cccgtcatca ctcaggaggt gggcgaggca
    10381 atgaccatca tcggtctggt ttccgccggt ctgggtgttt caattttgcc tgcttcattt
    10441 aaacgtgctc agctcaacga aatgcgctgg gtgccgattg ctgaagagga tgcggtttct
    10501 gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc cagcttcgcg taactttcgt
    10561 attcatctgc tgaatgctct caggtgaggg aaatttcagc gaaaaagccc gaaaaatgtg
    10621 ctgttaatca catgtctaag taaaaatttg acgacacgta ttgaagtgct tcaccatagc
    10681 ctacagatta tttcggagcg cgaaaatata gggagtatgc ggtggttgct gaaaaccagc
    10741 ctgggcacat tgatcaaata aagcagacca acgcgggcgc ggtttatcgc ctgattgatc
    10801 agcttggtcc agtctcgcgt atcgatcttt cccgtctggc gcagctggct cctgccagta
    10861 tcactaaaat tgtccgtgag atgctcgaag cacacttggt gcaagagctg gaaatcaaag
    10921 aagcggggaa ccgtggccgt ccggcggtgg gactggtggt tgaaactgaa gcctggcact
    10981 atctttctct gcgcattagt cgcggtgaga ttttccttgc tctgcgcgat ctgagcagca
    11041 aactggtggt ggaagagtcg caggaactgg cgttaaaaga tgacttgcca ttgctggatc
    11101 gtatcatttc ccatatcgat cagtttttta tccgccacca gaaaaaactt gagcgtctaa
    11161 cttcgattgc cataaccctg ccgggaatta ttgatacgga aaatggtatt gtacatcgca
    11221 tgccgttcta cgaggatgta aaagagatgc cgctcggcga ggcgctggag cagcataccg
    11281 gcgtaccggt ttatattcag catgatatca gcgcatggac gatggcagag gccttgtttg
    11341 gtgcctcacg cggggcgcgc gatgtgattc aggtggttat cgatcacaac gtgggggcgg
    11401 gcgtcattac cgatggtcat ctgctacacg caggcagcag tagtctcgtg gaaataggcc
    11461 acacacaggt cgacccatat ggtaaacgct gttattgcgg gaacctcggc tgcctcgaaa
    11521 ccatcgccag tgtggacagt attcttgagc tggcacagct gcgtctcaat caatccatga
    11581 gctcgatgtt acatgggcaa ccgttaaccg tggactcatt gtgtcaggcg gcattgcgcg
    11641 gcgatctact ggcaaaagac atcattaccg gggtgggcgc gcatgtcggg cgcattcttg
    11701 ccatcatggt gaatttattt aacccacaaa aaatactgat tggctcaccg ttaagtaaag
    11761 cggcagatat cctcttccct gtcatctcgg acagcatacg tcagcaggcc cttcctacgt
    11821 atagtcagca cattagcgtt gagagtactc agttttctaa ccagggcacg atggcaggcg
    11881 ctgcgctggt aaaagacgcg atgtataacg gttctttgtt gattcgtctg ttgcatggtt
    11941 aacatttttt aactgttcta ccaaaatttg cgctatctca atttgggcca ggaaagcata
    12001 acttagactt tcaaggttaa ttattttcct agtttatatt tgtgaagcat aacggtggag
    12061 ttagtgatgc tgaagcgttt ctttattacc ggtacagaca cttctgtagg gaaaacggtg
    12121 gtttcccgcg cattgctaca agcgttagcc tcccagggaa aaacagttgc gggatataaa
    12181 cccgtagcga aggggagcaa agagacaccc gaagggctgc gtaataaaga tgccctggtg
    12241 ttgcagagtg tttcaaccat cgaactgcct tatgaagcag ttaatcctat cgcgttaagc
    12301 gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt acaccctcat ttcaaacggc
    12361 ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag aagggactgg cggctggcgc
    12421 agtctgatga atgatttgcg tccactctct gaatgggtgg tgcaggaaca gttgccggtg
    12481 ttgatggttg tcggtattca ggaaggttgc attaaccatg cactgctaac agctcaggcg
    12541 attgccaacg acgggctgcc gctcattggc tgggtggcta accgaatcaa cccaggactg
    12601 gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac ttccggcacc gctcattggt
    12661 gaactgcctt atctgccgcg cgccgaacag cgagaactgg ggcaatacat ccgcttagct
    12721 atgctgcgca gtgtgctggc ggtagataga gtcacggtgt aacgtccgcg aaattaccga
    12781 cgcaatgacg caggcaatca ataaaccggg gagtagctga tactccccgg tcatttcaca
    12841 tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc gccaacagtg tcgccatctc
    12901 ggtcaatccg agtaaaagcg taatttcttc gccatcaggg aaccataatc ccaggctacg
    12961 accatacaac atgccaatgg caagaccgat aaatagcgtc ggtgtaaaga caccaccggg
    13021 tgcgccggaa ccgctactcg ccagcacggc acacagttta caaaggaaga tcccggcaat
    13081 gatcattaac agtggtgggg cggttaaaaa ggattgtacg gtgctatagc cgttgcccca
    13141 cactgcaggt gtgaacaggg aaagcagacc cacgatcaac ccgcctagtg ccagttgcca
    13201 gggcggcgca agtttgagac tcacaaatcc acgatgacag gcgttcatta acgttaacaa
    13261 cagtggtccg cacagacctg ccagcacacc tgtactgata atcagcgcat agtcacgagc
    13321 ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg tcgctatgat taatcagatt
    13381 gctaaccagc aatgccacga cggcggaaat aatcaccggg ccgagagagg ccaacatcat
    13441 agtgccaaac agcacttcgg ctataaataa actgccagca agcggggcac gataggccgc
    13501 agccattccc gccgcggcgc cacaggcgat ccataatttc cactcctggc gtggcgtaaa
    13561 acgttgggca aaacaggagg cggcaagggc agctaaaaga atcatcgcac cttcgcgacc
    13621 aattgcactg ccgctggtta ctaccagcag cgaggcaagc gatttaacca ggcttgctgc
    13681 gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa tcggtcggcg catgagggcg
    13741 ttgttgggta aatttctgcc agcccatcag caacaaaccc gccgccagtc cgccgagcgc
    13801 cggagttagc aaccgtcgcc agggggaaag gtttgtcgct gcattgacca gactgccgga
    13861 gtcattattg aggaacaacc actccagtag cagcatcgca tgacgaaacc cggcaacggc
    13921 aaaggccgcg agaataccga cgactgttgc gataagcaga cggcggaaca tagtgcgcag
    13981 atccggatag atgtgtagat gatgcatgag cagaaatggg caatgaaaca cgggcgacta
    14041 ttttgcggca aaagaggcca gccgcaaaag cagtgcgtgc tttaagagat aaaaaaacgt
    14101 gacactgtaa cctattattg ccggattgcg cgtaatcgtc accatccggc aatattacgg
    14161 tgatcctatc gaaacagcgg aggaattaac agttgtgatt gccattgcgc cagcgttaag
    14221 cgagacagtt cacccagtgc acggtagaaa gggtgttctg ctttttcgat aaaaacatca
    14281 agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca attcttcgca ttctgtctgg
    14341 cgaccattct ccgccaacca cgcagccatc aacagcagtg aaccaaaatg atcttccggt
    14401 tcgtattgct tcatttcaaa ctgaatgcct ttctcgcgca tccactgacg aagtgccaat
    14461 gttgaatcgc caaacagcac agattcgcga tccagccaga ccgaccccca cggcggagac
    14521 ggcagtgccc acgggccgac aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac
    14581 tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt ctggtagagg ccactgagtt
    14641 tcccagccgt cactggtgag taccgcaacc agaggtgcgg cttccgcact ctctggagcg
    14701 taataaaaca gcgcgcccaa cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa
    14761 tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg caccaatttg ttaacctgca
    14821 atagccattc ctactgtcat gtgcaggcca taaaagagca cacgcccgat catctcaccg
    14881 ccaagtatga ggattagccc cagaataagt ccggcaacgt gaggttcacg gcgacgaatg
    14941 agcgggcaaa gccagcatcc aagacttgcg cataacaata ccacacgcca gacctgtaag
    15001 gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg atgagtgaat agacgccaga
    15061 gacaaacctt gcagcacaat caccccggca caagcgatca gcgccagaac gctaatgatg
    15121 gcaaatggtg tggtattaaa tgtaacgcga gctgcccgca gaattgcggc agcaagtatt
    15181 ggaccgctca acaataccgt caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt
    15241 ggcacggtgt cgatttgata cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc
    15301 atgctgacaa gtaaccagag tttccccaac gcttgcggca ttttaccgat gaccgccacc
    15361 agccaccata gtccaccaac ggcaaaaaat atggaaccgg cagcaatctc gttactcaga
    15421 ccagaagctc cgattcgatt cagcgagtta aacgctcgca gtggcgatcc caggtgcatg
    15481 acagaggcaa taaacccgat gcccatcaac agccagagga aaaacatgcc acggacaata
    15541 cgctgacggt ctgcgtcatt tttggcggcg aaccagccaa ttccgctaac aatcagcgcg
    15601 cctactacgc attgacccaa tacggtaaag ataaccagtg gccattcatg ccatccattt
    15661 cccattttac acttcctccg gattagccag ataaccagtg g
//
LOCUS       DADLTD010000092        15470 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLTD010000092 DADLTD010000000
VERSION     DADLTD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.092"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..766
                     /locus_tag="JN701_002270"
     CDS             <1..766
                     /locus_tag="JN701_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7364713.1"
                     /translation="VRTVDSYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDP
                     LSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTIL
                     DMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFV
                     FMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFI
                     ASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
     gene            818..1753
                     /gene="ttcA"
                     /locus_tag="JN701_002271"
     CDS             818..1753
                     /gene="ttcA"
                     /locus_tag="JN701_002271"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY7364714.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLDVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1882..3241)
                     /gene="dbpA"
                     /locus_tag="JN701_002272"
                     /pseudo
     CDS             complement(1882..3241)
                     /gene="dbpA"
                     /locus_tag="JN701_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
     gene            complement(3687..4670)
                     /gene="zntB"
                     /locus_tag="JN701_002273"
     CDS             complement(3687..4670)
                     /gene="zntB"
                     /locus_tag="JN701_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY7364715.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4925..6157
                     /gene="dgcM"
                     /locus_tag="JN701_002274"
     CDS             4925..6157
                     /gene="dgcM"
                     /locus_tag="JN701_002274"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAY7364716.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6178..6741)
                     /gene="smrA"
                     /locus_tag="JN701_002275"
     CDS             complement(6178..6741)
                     /gene="smrA"
                     /locus_tag="JN701_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY7364717.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRNTAQAKQENWERHAKRSR"
     gene            complement(7071..7979)
                     /locus_tag="JN701_002276"
     CDS             complement(7071..7979)
                     /locus_tag="JN701_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7364718.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFHRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACIRLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8155..9455
                     /locus_tag="JN701_002277"
                     /pseudo
     CDS             8155..9455
                     /locus_tag="JN701_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
     gene            9455..10900
                     /locus_tag="JN701_002278"
     CDS             9455..10900
                     /locus_tag="JN701_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAY7364719.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            10937..12463
                     /gene="abgT"
                     /locus_tag="JN701_002279"
                     /pseudo
     CDS             10937..12463
                     /gene="abgT"
                     /locus_tag="JN701_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12474..12989
                     /gene="ogt"
                     /locus_tag="JN701_002280"
     CDS             12474..12989
                     /gene="ogt"
                     /locus_tag="JN701_002280"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY7364720.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13184..13936
                     /locus_tag="JN701_002281"
     CDS             13184..13936
                     /locus_tag="JN701_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY7364721.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14088..15038
                     /gene="uspE"
                     /locus_tag="JN701_002282"
     CDS             14088..15038
                     /gene="uspE"
                     /locus_tag="JN701_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY7364722.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15088..15346)
                     /locus_tag="JN701_002283"
                     /pseudo
     CDS             complement(15088..15346)
                     /locus_tag="JN701_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         3951 a         3863 c         3809 g         3847 t
ORIGIN      
        1 cgtgcgcaca gttgatagct atatcgccct gctctgttcg ttgttacgtt ttgcgtatca
       61 gtcgggattt atatcaacca aaccatttga aggagtaaaa aaattacagc gaaacagaat
      121 aaaacctgac ccgttatcta aaacagaatt caatgcatta atggaaagtg aaaaaggaca
      181 gagccaaaac ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg aactggcagc
      241 tctggcgtgg gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa gaaacctgac
      301 gatacttgat atgttcggtc ccccaaaaac aaatgccggg atccggacgg taacattact
      361 gcagccggct cttgaagcac tgaaggagca atacaaactg accgggcatc atcgcaaaag
      421 cgaaatcact ttttatcatc gggagtacgg cagaactgaa aagcaaaaac tgcattttgt
      481 tttcatgccc agggtgtgta acgaaaaaca gaaaccttat tactcggtaa gcagtttggg
      541 tgcaagatgg aatgcagcag taaaacgtgc tggtattcgc cgccgtaatc cgtaccatac
      601 gcgacatact tttgcctgct ggctgttgac ggcaggagcg aacccggcat ttatagccag
      661 ccagatgggg catgaaactg cgcaaatggt gtatgaaatt tacggtatgt ggattgatga
      721 catgaacgac gaacagatag ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc
      781 cccaatttgc cccatttagt accagagaac tgaaataatg caagaaaatc aacaaattac
      841 aaagaaagaa caatacagcc tgaacaaatt acaaaaacgt ctacgtcgta acgtgggcga
      901 agccattgct gacttcaata tgattgaaga aggcgatcgc atcatggttt gcctctcagg
      961 gggtaaagac agctatacca tgctggagat tctgcgcaat ttgcagcaaa gcgcgccaat
     1021 caatttttcg ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc cagaacacgt
     1081 tctgcccgtg tatcttgaaa agctggacgt tgagtacaag attgtcgaag agaacactta
     1141 cggtatcgtg aaagagaaga ttccagaggg caaaaccact tgctcactgt gttctcgcct
     1201 tcgtcgcggt atcctttatc gtaccgcaac ggaactgggg gcgacgaaga tcgcgctggg
     1261 tcaccatcgt gacgatatcc tgcagacgtt gttcttaaat atgttctacg gcggcaagat
     1321 gaaaggtatg cctccgaaac tgatgagcga tgatggcaaa catatcgtta ttcgtccgct
     1381 ggcctactgc cgcgagaaag atattcagcg atttgccgat gcaaaagcgt tcccgattat
     1441 tccgtgcaac ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg ctgacatgtt
     1501 gcgtgactgg gataaacgtt atccagggcg tatcgagacg atgttcagcg cgatgcagaa
     1561 tgtggtgccg tcgcatctgt gcgataccaa cctgttcgat ttcaaaggca tcacccacgg
     1621 ttctgaagtg gttaacgggg gtgatctggc gtttgatcgc gaagagatcc cactacaacc
     1681 ggcgggctgg cagccagaag aagatgaaaa tcagttggat gagttacggc tgaatgtggt
     1741 tgaagtgaaa taaccaggat agcgcccgat gcgcaagcgt atcgggctac tcttatggag
     1801 gccggataag acgcggccaa cgtcgcatcc ggcaatgccg aagaagatgt ttactcttgc
     1861 acccggcaat tcaacatttc attattttaa taaccgcacc cggcacgttt ttcctttaat
     1921 cttcccgccc tgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata
     1981 gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatatctcc
     2041 tgtcagtgca cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca
     2101 caacgttgcc atttctgctt ccagcggcac aatggaacta ttagctggcg gcgtttgcca
     2161 gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggagc
     2221 acagaaactg atcgccagac cgctatttcc tgcacgagct gtacggccga tgcgatgtac
     2281 atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatcag
     2341 caacatcagt cgcaaccagt acacgggcgc taccgttagc aaaacgtacc agggtctgat
     2401 cgcgatcgcg ttgctccaaa tcgccgtgta atgacaatgc actttgccct acttcattca
     2461 gcgcgtcgca gacagcctgg caatcttttt tggtattgca aaacaccacg caagaggatg
     2521 gctgatgcaa gcttaataac cgttgcaaca gaggaatttt gcctttgctg gatgtctcat
     2581 aaaattgttg ttcaatgggt ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat
     2641 cgcgttgcac tcgtccgctg attacggcga tggcttccgg ccaggttgcc gaaaacagaa
     2701 gcgtctgtcg agatgcaggc gcaaaacgga tgacatcatc aatggcatcg ctaaatccca
     2761 tatccagcat gcggtcggcc tcatccatca ccaaagtatt cagcgcatcc agtgataccg
     2821 tgcctttttg caagtgatcc agcaaacgac ccggcgttgc cacgataata tgcggcgcat
     2881 gttgcaacga atcacgctgc ataccgaacg gttgaccacc gcacaacgtc aaaattttgg
     2941 tatttggcag aaaacgcgcc agccgacgca attcacctgc cacctgatcc gccagttcac
     3001 gcgtaggaca cagcactaaa gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca
     3061 agccgaggcc aaaagccgcc gttttgccgc tgccggtttt cgcctgcacg cgaacatctt
     3121 ttccggcaag gatcgccgga agcgcggcgg cctgcaccgg cgtcatagtt aaataaccca
     3181 actcattaag gttcgtgagt tgggcgggag gcaaaacatt cagggtagaa aaagcggtca
     3241 caatctattc tcgtggtcat cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg
     3301 cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact ggaacaggat
     3361 cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc atatttccct
     3421 ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca aaaaaaagcc
     3481 gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa
     3541 gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg caagggagat
     3601 attgccctga atgggtagag agtttattga cttcgctcaa actttgcggc gtttttgtat
     3661 acagacagcc ggaaaaattg cttttgttac aaccatttac tacgatgcaa ccataaagca
     3721 acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccgaa ttgccacccg
     3781 ccgccaggga tcccaccaag gttgacgcca aataaccctg tcagaaaggt actgggtaaa
     3841 aagaccattg ccatcaatga cattgtatag gtacgacgag ctaaattttc ctgcatcacc
     3901 tgagcgattt catccgccat cacgccagtc cgtgctatac aggcgtcgat ttcgtcaaga
     3961 ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc gctcatccac
     4021 ggcaaacgtt cactggcaag acgagcataa acatcacgtt gcggtgccat atagcgacgc
     4081 atcacgatta attgtttgcg cagcagagcc aggaatccac gcggtggaat ttgctgatca
     4141 aggagattat cttcaaggtc gataatttta tcgtgcagct gctcgataaa ttcgctggaa
     4201 tgatcggtca acgcatcgca cacatccacc agccaccctc cgcaatcggt cggacccgtg
     4261 ccctcttcca gatcgctcac cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa
     4321 acaattaacc gcccgtccat atatacacgc atggcgacca gttgatcggg gcgttcatcg
     4381 gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgagacg gctgactcgg
     4441 ggccgtgtgc tctcgcctgc cagcgcatca cgtacgttat tgggaagcag cggtgttgtc
     4501 gccagccatt gggcgctatc atggtgtaca taattaaggt ggagccagca gggatgcgct
     4561 tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc cagcatccag
     4621 gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac agtgcctcca
     4681 tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca tctcatcacc
     4741 ccattaccct gaaatgatta ataaaattct gtctaaattg aatacaaaaa gcaaaatgct
     4801 tttccgtata caaaccgtgt gaagtgttaa atagcgtcta tcattatcag aattatctga
     4861 tcatatgacg tggctttttt gcgatcggat agcaacaaaa attgataaaa ataacgggat
     4921 ctcaatgatt acgcacaact tcaataccct ggacttactc accagtcctg tctggattgt
     4981 ttcgcccttt gaggaacagt taatttatgc caatagcgcg gcgcgactgt tgatgcaaga
     5041 cctcacgttt agtcagctac gaaccggacc ctattccgtc tcctcacaaa aagaactgcc
     5101 gaaatacctc tccgatctgc aaaaccaaca cgatattatc gaaatcctca ctgttcagcg
     5161 taaagaagag gaaacagcat taagctgtcg gcttgttttg cgagagctga cagaaacaga
     5221 accggtgatt attttcgaag gtatcgaagc gccggcaacg ctgggtttaa aagccagtcg
     5281 ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg cgcttttttc tgactaactc
     5341 tgcaccaatg ttgttgattg acccctcacg agatggccaa atcgtcgatg ctaacctcgc
     5401 cgcgctcaat ttctacggtt ataaccatga aacgatgtgc cagaaacata cctgggaaat
     5461 taatatgctc gggcgtcgcg tcatgcctat catgcatgaa atttcgcatt tacccggcgg
     5521 tcataaacct ttgaattttg ttcataaact ggcggatgga tctactcgtc atgtgcagac
     5581 ctatgccggg ccgattgaaa tttatggcga caagctcatg ttatgtattg tgcatgatat
     5641 tactgagcaa aaacggctgg aggagcagct ggaacatgct gctcaccatg acgcgatgac
     5701 cggattactg aatcggcgac agttttatca cattacggaa ccaggccaaa tgcagcacct
     5761 cgccatcgcc caggattaca gcttgttgct catcgacacc gatcgtttta aacacattaa
     5821 cgatctctat gggcattcta aaggtgatga ggtgttatgc gccctcgccc gcaccctcga
     5881 aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga ggcgaagagt ttgtcttatt
     5941 gctaccaaga accccactgg ataccgcgct ttcgctggct gaaactatcc gtgtaagcgt
     6001 ggccaaagtg agtatttcgg gcttaccacg ttttaccgtc agcattggtg tggcgcatca
     6061 cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt gatgatgctt tgtatcgggc
     6121 gaaaaatgat ggacgcaacc gcgtgctggc ggcataagcc gcggatgcgt ctcgagatca
     6181 acggctgcgc ttagcgtggc gctcccagtt ttcttgcttc gcctgcgccg tgttacgtag
     6241 tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc gcggtgcaat atgcctgaac
     6301 atcatcaaat tcggtcagcc agcgcgccac atagctgcgg acaatattgg catgcgattt
     6361 atcttcccgc cctttaccat gaataatcag cacattacgc aaaccatccg ccagggcttg
     6421 gtgaatgaaa ctgaacacca ttttgcggca ctcttccacc ggctggcgca aaagattcag
     6481 gctcgcctgt tgcggatatt taccactgcg cagcttatcc agcaccccat gttgcaaccc
     6541 ttcccgccga aactccagcg gctgacttag cgggatgatg tcgagaaatc cggtggtgag
     6601 gaaattatca agctgcagcg tgtcgatacg ctgcggcgca cgttggttac gcgttggatg
     6661 ccagtggaca tctgtagcac gtttcagcgg ctggacatct tccatggcgt caagaaacag
     6721 cgatttgtcg tcaaggttca tgttacatcc tcccgcaatt aagagcgcga tatgataacc
     6781 agaccgggtc ggtccaacaa cgtattacca aaaattccag taataagttc caaatattgc
     6841 cgatatttta agcaaaatgc ttatgcatga ttattcattc acgatattaa taatgtaact
     6901 tatattttcg tgaaatctgt cactgaagaa aattggcaac taaaggttaa aaccgttata
     6961 acacagtcac cggcgcagag gagacaatgc cggacttaag acgcggatgc actgctgtgt
     7021 gtactgtaga gtctggcgga tgtcgacaga ctctattttt ttatgcagtt ttaactttgc
     7081 agatagccgc attctcgccg gaattgcgtg attaatgacg gggtcagtgg tgtctggcga
     7141 ctatcacgcc gctgaatcaa ataataggcc gctttcggta aaatttcgct aaccggcaac
     7201 atcaccagcc cctgtccgtg caagggatcg cagcccattt cttcaggcag tatgctgaga
     7261 aaatcgcttt ttgccaccag cctgatacag gctgagaacg tctcgcagac tacaccgacc
     7321 tgtggcgttt gcgcctgatc gtcaagcaat tcactcaact gtttgtagta actgccgtgt
     7381 ggcgtcggca ttgtccagct gtaatccagt aactgtttga tcgaacgggc accaatggcg
     7441 gggtgtcccg ggcggcaaaa gatcgcgaat tgcttttcca gtaatttctc aaaagtaaat
     7501 tcgtggtcgt acggtccctg ataataggta ttgatggtga aatccaattc tccctgacgc
     7561 aattcattaa tcatcgacac cagttgccct tccataatgc gtacttttac ctgcggatgc
     7621 tgctgatgaa aacgagatat gacagctggc atcagactgc gggaaatact ggcccccatg
     7681 ccgatattaa tctgccctgc cagttgccct tgtcgttggc gaatatcctc ttgggctgcg
     7741 cgcagctctt caagaataag actggcgtgc tgataaaaac tttcaccggc atcagttaac
     7801 gtcacgcctt tactacggtg aaaaaagagt tgcgccgcta atccttcttc caactcctga
     7861 atagatttac tcagtgccgg ttgcgacata ttcaacattc ggctcgctcc gcgaatgctg
     7921 ccctgacgag ccacttcaac aaaagcacga atttgatgaa tttttacctg aaaagccatg
     7981 acgccaccga taaccgttat ttatcagacc aaagaaactg gcatctactt taatgcagat
     8041 gattgtgtca gggtaattta tgaacggtta aaactgtgaa aaatcagtta gtgataagta
     8101 aaaactatcg ctacgtgaac cgggtcacac tttttactga tgacgggaaa ggttatggag
     8161 tctttgaatc aatttgttaa ttcgcttgcc ccaaaattat cgcactggcg acgtgatttt
     8221 catcactatg cagagtctgg ctgggtggaa ttccgcactg ccacccttgt tgcggaagaa
     8281 ttgcagcagc tcggctattc actggcgctg ggccgcgaag tagttaatga aagtagccgg
     8341 atgggactac ctgatgaact cactctacaa cgcgaattcg agcgcgctcg tcaacagggt
     8401 gcgctagcac aatggattgc ggtttttgaa ggtggtttca ctggtatcgt cgccaccctg
     8461 gataccggtc gccccggtcc ggtgatggct ttccgtgtcg atatggacgc gctggatctc
     8521 agtgaagagc aggatgtcag ccatcgcccc taccgcgacg gttttgcgtc atgtaacgcc
     8581 ggaatgatgc atgcctgtgg tcatgatgga cataccgcca ttgggcttgg gctagcgcat
     8641 acccttaaac aattcgagtc cggactacat ggcgtcatca aactgatttt tcagcctgca
     8701 gaggaaggta cgcgtggcgc gcgggcgatg gtcgatgcag gtgtcgtaga tgatgttgat
     8761 tattttactg ccgtgcacat tggcactggc gtacctgcgg gcaccgtggt gtgcggcagt
     8821 gataatttta tggcaaccac caaatttgac gcgcacttca ccggtaccgc cgctcacgca
     8881 ggcgcaaaac cagaagacgg tcacaatgcc ttgttggcgg cagcacaagc cactcttgca
     8941 ctgcatgcaa tcgccccgga gcttccagag taaacgtggg cgttatgcag gcaggaagcg
     9001 gtcgtaacgt tgttcctgcc tcggcgttgc tgaaagtgga aacacgcggg gccagcgacg
     9061 tcattaatca atatgttttt gaccgtgcac aacaagcgat tcagggcgca gcaaccatgt
     9121 atggtgtcgg cgttgaaact cgtctgatgg gtgcagctac cgccagttct ccttcgccgc
     9181 aatgggtcgc atggttgcaa agtcaggcgg ctcaggtcgc ggggggcaat caggccattg
     9241 aacgtgttga agcgcctgcg ggttccgaag atgccacatt aatgatggcc cgcgtgcagc
     9301 aacatcaagg gcaagcctcc tacgtggtgt ttggcacaca gctggcggca ggtcatcaca
     9361 acgaaaaatt cgattttgac gagcaggttc tcgctattgc cgtcgaaacg ctggcgcgca
     9421 ccgcgctcaa ttttccctgg acgcgaggta tctgatgcag gaaatctatc gttttatcga
     9481 cgatgcgatt gaagccgatc gccaacgtta taccgatatt gccgatcaaa tctgggatca
     9541 tccagaaaca cgttttgaag agttctggtc agcggagcat ctggcttcgg cgctggaatc
     9601 tgcaggcttc accgttaccc gcaacgtagg ctatatccca aatgccttta ttgcttcgtt
     9661 tggtcaaggc aaaccggtta tcgccctgct gggagaatat gacgccctgg caggtttaag
     9721 tcagcaagca ggttgcgcgc aacctacatc cgtgacgccc ggtgaaaatg gtcacggttg
     9781 cggacacaat ttgctgggaa ccgccgcctt tgccgctgca atagccgtca agaaatggct
     9841 ggaacaatat gggcaaggcg gcacggtgcg cttttatggt tgtcctggcg aagaaggcgg
     9901 ctcgggtaaa acgttcatgg ttcgcgaggg ggtatttgat gatgtggatg cggcactcac
     9961 ttggcacccg gaagcctttg ccggtatgtt caatacccgc acgctggcaa acattcaggc
    10021 atcatggcgc tttaaaggga tcgcagcaca tgccgcgaat ttccctcatt tgggacgcag
    10081 cgcccttgat gccgtaacgt tgatgaccac tggcaccaac ttcctcaacg aacatattat
    10141 tgaaaaagcg cgcgtacact atgccatcac aaatagcggc gggatctcgc ccaacgtggt
    10201 ccaggcgcag gcagaagtgc tttatcttat ccgcgccccc gaaatgaccg acgtgcagca
    10261 tatttatgat cgggtcgcca aaatcgccga aggtgcggca ttgatgaccg aaaccacggt
    10321 tgaatgccgc ttcgacaaag cctgttccag ttatctcccg aatcgcacct tagaaaatgc
    10381 catgtaccat gccctatccc attttggtac cccggaatgg aactccgaag aactggcttt
    10441 tacgaaacaa attcaggcta cgctcccccc caacgatcgg caaaacagtc tgaataatat
    10501 cgccgcaacc ggtggcgaaa acggcaaggc ttttgcacta cgtcatcgtg aaacggtact
    10561 ggcgaatgag gtcgctccat atgccgccac cgataacgtg cttgcggcat cgactgatgt
    10621 cggcgacgtc agttggaaac tgcctgttgc ccagtgtttc agcccctgtt ttgccgtcgg
    10681 tacaccgcta catacgtggc aactggttag ccaggggcga acatctattg ctcataaagg
    10741 aatgctgctg gcggcgaaaa ctatggcagc aaccacactc aatctcttca ttgattcagg
    10801 gctattgcaa gaatgccaac aagagcatca gcaagttacg gacacgcaac cgtatcactg
    10861 ccctatccca aaaaacgtga cactgtcatc tttaaaataa caacaacaac gcaaacacaa
    10921 caaccgagga atgcccatga gtatgtcatc cataccgtcg tcctcctaat ccgggaagct
    10981 ctatggctgg gtcgaaagaa ttggtaacaa ggttccccat ccttttttgc tctttatcta
    11041 tttgattatc gtactcatgg tgacgacggc aattttgtcg gcctttggcg tcagtgcgaa
    11101 aaacccgacc gatggaacgc cggtggtggt gaaaaacctg ctcagtgtgg aaggattaca
    11161 ctggttttta cccaatgtta ttaaaaactt tagcggtttt gctccacttg gtgcgatcct
    11221 ggcgctggtt ttaggtgccg gtctggcgga gcgcgtcggc ttactgccag cgctaatggt
    11281 taaaatggca tcgcatgtta atgtccgcta cgccagttat atggtgctgt ttattgcttt
    11341 tttcagccac atttcttccg atgcggcgtt agtgatcatg ccaccgatgg gtgcgctgat
    11401 ttttctggcg gtgggcaggc atccagttgc aggtttactg gctgccattg caggcgtagg
    11461 ttgcggcttt acggctaatt tactgattgt cacaaccgac gtgttgctgt cggggatccg
    11521 cacggaagcg gcggctgcgt tcaatccgca aatgcacgtc agtgtaattg ataactggta
    11581 ttttatggcc agctccgtag tcgtactgac gattgttggc ggcctgataa ccgacaaaat
    11641 catcgagcca cggttaggtc aatggcaggg aaacagcgat gagaaactgc agacattgac
    11701 cgaaagtcag cgttttggtt tacgcatagc aggtgtcgta tcgctacttt ttattgctgc
    11761 gatcgcgctg atggtgatcc cggaaaacgg gatattgcgc gatccgatta atcacaccgt
    11821 gatgccatca ccctttatta aaggtatcgt gccactaatc attctttttt tctttgtggt
    11881 ttcgctggct tatggcatcg ctacccgcac aattcgacat caggcggatt taccgcattt
    11941 aatgattgaa ccgatgaaag agatggcggg atttatcgtg atggtttttc ccctcgccca
    12001 gtttgtcgcc atgtttaact ggagcaacat ggggaaattc atcgccgtgg ggctgaccga
    12061 tatactggaa agttcagggc ttagcggcat cccggcgttt gtcggtctgg cgttgctttc
    12121 ctctttctta tgcatgttta ttgccagtgg ttccgcaatc tggtcgattc tggcccccat
    12181 tttcgtacca atgtttatgc tacttggctt tcacccggca tttgcgcaaa tcctctttcg
    12241 tattgccgac tcatccgtat tgcctttagc gccggtatct ccttttgttc cactgtttct
    12301 tggattcctg caacgctaca aaccagacgc gaaactgggt acttactatt cgttagtctt
    12361 gccctatccg cttatctttt tggtggtatg gctgctgatg ttgctggcgt ggtatcttgt
    12421 tggtctgccg ataggtccgg ggatttaccc acgtttgtct taagagagaa cggatgctga
    12481 gattacttga agaaaaaatt gccacgccac tgggtccact gtgggtgatt tgcgatgagc
    12541 aatttcgcct gcgggcggtt gaatgggaag agtacagcga acgcatggtg cagctgctgg
    12601 acatccatta tcgcaaagaa ggctatgagc gcatttctgc caccaaccca ggcggtttaa
    12661 gcgacaagct tagtgattat tttgccggta atcttagcat tattgatacg cttcccactg
    12721 ccacaggggg gacgccattt cagcgcgaag tctggaaaac tctacgcact atcccctgcg
    12781 ggcaggtaat gcattacggc caactggctg aacaattggg ccgccctggc gcggcgcgtg
    12841 ccgttggtgc ggcaaacgga tcgaatccca tcagcatcgt cgtaccttgc catcgggtta
    12901 ttggccgaaa cggcaccatg accggatatg caggcggagt tcagcgaaaa gagtggttat
    12961 tgcgccacga aggttatctt ttgctgtaaa cattaaacaa tttgtgccag cttgttcaca
    13021 cttttatgta aagttaccct taacaactta atggttttca aatagataga catatattta
    13081 catctaatat cggaattctc tgctgttaag gtttgcttag acttacttgc tccctaaaaa
    13141 gatgttaaaa ttgacaaata tcaattacgg cttgagcaga cctatgatcc cggaaaagcg
    13201 aattatacgg cgcattcagt ctggcggttg tgctatccat tgccaggatt gcagcatcag
    13261 ccagctttgc atcccgttca cactcaacga acatgagctt gatcagcttg ataatatcat
    13321 tgagcggaag aagcctattc agaaaggcca gacgctgttt aaggctggtg atgaacttaa
    13381 atcgctttat gccatccgct ccggtacgat taaaagttat accatcactg agcaaggcga
    13441 cgagcaaatc actggtttcc atttagctgg cgacctggtg ggatttgacg ccatcggcag
    13501 cggccatcac ccgagcttcg ctcaggcgct ggaaacctcg atggtatgtg aaatcccgtt
    13561 cgaaacgctg gacgatttgt ccggtaaaat gccgaatctg cgtcagcaga tgatgcgtct
    13621 gatgagcggt gaaatcaaag gcgatcagga catgatcctg ctgttgtcga agaaaaatgc
    13681 cgaggaacgt ctggctgcat tcatctacaa cctgtcccgt cgttttgccc aacgcggctt
    13741 ctcccctcgt gaattccgcc tgacgatgac tcgtggcgat atcggtaact atctgggcct
    13801 gacggtagaa accatcagcc gtctgctagg tcgcttccag aaaagcggta tgctggcagt
    13861 caaaggtaaa tacatcacca tcgaaaataa cgatgcgctg gcccagcttg ctggtcatac
    13921 gcgtaatgtt gcctgatttt tccgcataac tcactatcct tctgtcatat cattaaattt
    13981 ttctgattta ttgatctggc agaaggttca tcactgtttc attcaccaga tatgggttaa
    14041 tcttttaatt acaaactgcg ttgacagttg ttgtaaggag accctgtatg gctatgtatc
    14101 agaacatgct cgttgttatc gatcctaacc aggacgacca accagcattg cggcgagctg
    14161 tttatttaca tcaacggatt ggtggcaaaa ttaaagcctt tttgccgatc tatgacttct
    14221 catacgaaat gaccaccctg ctctccccgg acgaacgtac cgctatgcgt cagggcgtca
    14281 tcagccagcg tacagcctgg atccacgagc aggcaaaata ttatctcaat gctggcgttc
    14341 ccattgaaat taaagtggtc tggcataacc gtcctttcga agccatcatt caggaagtga
    14401 tcagcggcgg gcacgatttg gtgctaaaaa tggcgcacca acatgaccgt ctggaagcgg
    14461 tgatttttac gccaacggac tggcatctgt tacgcaaatg cccaagcccg gtgtggatgg
    14521 tgaaagacca gccgtggccg gaaggaggta aggcgctggt ggcggtgaat ctcgccagtg
    14581 aagagccgta tcataatgcg ctcaatgaaa aactggtcaa agagacgatc gaactggcag
    14641 aacaagtcaa ccataccgaa gttcatctgg ttggcgctta cccagtaacg ccaattaata
    14701 tcgcgattga actgccggaa tttgagccga gcgtttataa cgatgccatt cgtgggcaac
    14761 atttgctggc aatgaaagcc ctgcggcaga aattcggcat taatgaaaac atgacgcacg
    14821 tagaaaaagg tctgcccgaa gaggttattc ctgatttggc ggagcactta caggcgggga
    14881 ttgtggttct gggcacggta ggacgcaccg gtatttcagc agcattcctc ggcaacacgg
    14941 cggaacaggt gattgatcat cttcgctgcg atctgttggt cattaaacct gaccagtatc
    15001 agacacccgt tgaactggat gacgaagaag acgattaacg actcttcaat aactgacaag
    15061 gcccgagtta tcgggcctat tttttactta ctgctcttcg ccgccaagga aataaattcc
    15121 caacgggatt gccagcaaca cagtaaagac caggctataa acaaaaatca tcgatgactg
    15181 aatcacatac attgatgtcg tccactcaga cagcggaata ttgtactgtt ctataacgcc
    15241 gccgatagtc gcgcgagtca caaccgacgc cgcaataatg acaaaccgcc aacaaaccaa
    15301 agagaaattt cttccctttc gctgacttca gtttcgccat aatcatgatg taatccttgt
    15361 gcagacaaat ttcttgttac ctaaacgtaa caataccatg acatttcacc gttgtctgct
    15421 ctgaaaacct cacctcttag ctggatttac aatcaacgca ttgattagtt
//
LOCUS       DADLTD010000093        15302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLTD010000093 DADLTD010000000
VERSION     DADLTD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.093"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..321)
                     /locus_tag="JN701_002284"
     CDS             complement(<1..321)
                     /locus_tag="JN701_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY7364723.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVR"
     gene            complement(326..511)
                     /locus_tag="JN701_002285"
     CDS             complement(326..511)
                     /locus_tag="JN701_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY7364724.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(508..3147)
                     /locus_tag="JN701_002286"
     CDS             complement(508..3147)
                     /locus_tag="JN701_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY7364725.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3355..4344
                     /gene="ldhA"
                     /locus_tag="JN701_002287"
     CDS             3355..4344
                     /gene="ldhA"
                     /locus_tag="JN701_002287"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7364726.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4455..4877
                     /gene="hslJ"
                     /locus_tag="JN701_002288"
     CDS             4455..4877
                     /gene="hslJ"
                     /locus_tag="JN701_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY7364727.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4874..5140)
                     /locus_tag="JN701_002289"
     CDS             complement(4874..5140)
                     /locus_tag="JN701_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY7364728.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5414..8938
                     /gene="nifJ"
                     /locus_tag="JN701_002290"
     CDS             5414..8938
                     /gene="nifJ"
                     /locus_tag="JN701_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY7364729.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9304..10437
                     /gene="ompN"
                     /locus_tag="JN701_002291"
     CDS             9304..10437
                     /gene="ompN"
                     /locus_tag="JN701_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAY7364730.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGTAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10578..11012
                     /gene="uspF"
                     /locus_tag="JN701_002292"
     CDS             10578..11012
                     /gene="uspF"
                     /locus_tag="JN701_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY7364731.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11593..12483
                     /gene="sitA"
                     /locus_tag="JN701_002293"
     CDS             11593..12483
                     /gene="sitA"
                     /locus_tag="JN701_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY7364732.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            12483..13310
                     /gene="sitB"
                     /locus_tag="JN701_002294"
     CDS             12483..13310
                     /gene="sitB"
                     /locus_tag="JN701_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY7364733.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13307..14164
                     /gene="sitC"
                     /locus_tag="JN701_002295"
     CDS             13307..14164
                     /gene="sitC"
                     /locus_tag="JN701_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY7364734.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14161..15018
                     /gene="sitD"
                     /locus_tag="JN701_002296"
     CDS             14161..15018
                     /gene="sitD"
                     /locus_tag="JN701_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY7364735.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         3845 a         3848 c         3701 g         3908 t
ORIGIN      
        1 tcgtacccat ttaccgttta tcccttgtac atattccccc ggtttggccc gagctacgag
       61 cttttgcccg gcgagtttcg cgatatcatc tacagacaca ttattttgct ttgccagttg
      121 ttggtaactc gctttacgtt cggcattaat gtcggttact aatttctctg tttcagcatc
      181 cgttttcagc gcaaccagat aaccataaaa tgtttccccg acccgcccct gcgttctggc
      241 ttcatccaga gtcaatgcca ttacattgct gcttaccagc ccaacaagaa acgcacaaag
      301 aagtaatgtt ttcttcatca tcgcctcagt aaagatcgct acgagtttca agcagctctt
      361 cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc atattgatag
      421 tgatcggttc cttgggcgca gcgacttcaa tacgcggcgt acaggcagcc agcataaaag
      481 atgacgtcaa cgcagccagt aaaattttca ttgtttttcc tcacactctt ttccttgcgg
      541 acagtcattt cccggcagac gtgcgttctg ctccaaccat gcctggagat tatcgccaaa
      601 gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa ggtttaccgt
      661 accgctttta ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg ttaataaacc
      721 gagattatct aaattaatat ttgtcgatga acggctaatt tccatatagc gcaaccagtt
      781 aattgctgaa cccgcagtca tattgtcttt caccaccgca tctgcggtgt ctttatccag
      841 ccgcaaggtc attggcccgc tatttgccag ccaaccatct ttcactatcc atttttcgtt
      901 gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt gtttcggatt
      961 gacggcgcta accagttcgc tggatgagag attattcagc cgcaacagcg ccgggtcatg
     1021 ttgcggcata cgtaattgtt tcatcagtac gttaccgcct aacacatcga cgctaacatc
     1081 agtcaacagc aagggttctt cttcagtcca cggataacgc ccttgcaaat cagccgtaat
     1141 atttttcgct gtcaccagat taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt
     1201 ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga caccgttaac
     1261 ctgattatct ggcatccagg cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa
     1321 tccttgttca ggcgcagcag aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt
     1381 catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt ttggccacca
     1441 ggcgttaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg ggccaatctc
     1501 accagcatgt aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat cgacgctaaa
     1561 ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc cggcgtccag
     1621 tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt acccagacga cgggcttgtc
     1681 cagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt
     1741 ggagaggtcg gtcagcgtaa tcgtgctgtc atgccattca ccttttcctg cgacatccca
     1801 ggtggcattc atcggtgtaa aactgccttt tccccagtag cgccactgcc agcggcccgc
     1861 gtcagggaga aaatcattcg ccagcccatc catatgcagc acgaaatcgc ccagttcatt
     1921 ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg tgacttttac
     1981 acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc ttccctttga
     2041 acgaagtaac gcgcctggct caaaggccag cgttgggtca gtcagacttc cacttagctg
     2101 cgcgggtaag cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag
     2161 aggcagctgg ctgttatcca tgcttaattt tcctgggcca aaatttagca ccgcgttgcc
     2221 tttacccgct tgcccctggg tcagcacact cagtcgtccg ctgatcagag cgttctcaag
     2281 ccctgcctgc cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa aacctgcata
     2341 cggccaactc cagcgaccat cgcttacggt caattgttga cgagtaattt gccacggcaa
     2401 atcgagcaac ggatcgccgt tatcacgtgc cagcacaatc aattgcccgc tattttcctg
     2461 ccagtccagc tctgcatcca ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc
     2521 atgcccactt acaggaagtc catccggcac gagcggcata gtaaattccc ccaccagttt
     2581 aaccggcggc tgatattcaa acgcgatgac atccagttcg ctgactgtaa gttgttgccc
     2641 tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct ggatatcaga
     2701 ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc
     2761 gatattgatc caggtgttag gcagcatgga ctgccactgc gcgagggttt ttggagcagc
     2821 tggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc
     2881 gacgttcaat aaccagcggc tgggatgtga aaggctggcg ttggtgatat gcgcaagctg
     2941 acaatctccc accagataac ggagatcggg aatgattaaa cctttacgcg taatgcgtgg
     3001 gctttcatct aatgcaatac gtgtcccgag cggtagccag atgcccgcca gcgtgggaac
     3061 ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga ataatcagta ataacagcgc
     3121 gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag cgaaaaatta
     3181 agcattcaat acgggtattg tggcatgttt aaccgttcag ttgaaggttg cgcctacact
     3241 aagcatagtt gttgatgaat ttttcaatat cgccatggtt ttcaattata tttgaaattt
     3301 tgtaaaatat ttttagtggc ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa
     3361 ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc
     3421 tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc
     3481 aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc ggtgctggaa
     3541 gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc
     3601 gaccttgatg cggcaaaaga actggggctg aaagtagtcc gtgttccagc ctatgatcca
     3661 gaggccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc
     3721 gcgtatcagc gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg
     3781 tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt
     3841 ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc agcggcgctg
     3901 gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct
     3961 ctgcactgcc cgctgacacc ggcaaactac catctgttga acgaagccgc cttcgatcag
     4021 atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggca
     4081 gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt gtatgagaac
     4141 gaacgcgatc tgttctttga agataaatcc aacgacgtga tccaggatga cgtattccgt
     4201 cgcctgtctg cctgccacaa cgtgctgttt accgggcacc aggcattcct gacagcagaa
     4261 gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggcgaa
     4321 acctgcccga acgaactggt ttaatgttgc agctcccctg caatccaggg gagctgattc
     4381 agataatccc caatgacctt tcattctcta ttcttaaaat agtcctgagt cagaaactgt
     4441 aattgagaac cacaatgaag aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg
     4501 gatgtgtaag taatgacaaa attgctgtta cgccagaaca gctacagcat catcgctttg
     4561 tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca gaaatcagtt
     4621 ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac
     4681 tgtctaatgg tgaactgaca gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc
     4741 cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg
     4801 atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact tataagctgg
     4861 cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt tcgctacagc
     4921 gtttgccgtt gggtaatgca cacatcccaa tcgccgtgcc atccagttga cgggcaacag
     4981 aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc gacgctttta
     5041 aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta cacgccgaca
     5101 acaataaagc ggcacaccct acccaaaacg ctgctcgcat cttttcttcc tctgatcttc
     5161 aagccaaacg acaccgccat aaataatagg cagcacagag ggcgacatcg agagccgtcc
     5221 tgcgcgttgc cccgcatttt tactttttta tggctatttt tttgccctct gtttgatcaa
     5281 aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa agccatttgc
     5341 taaaattgaa agattactac tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat
     5401 gtaagggtgt catatgatta ctattgacgg taatggcgcg gttgcttcgg tggcgtttcg
     5461 caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg cagaacaggc
     5521 tgatgcctgg gccggaaacg gcttgaagaa cgtttgggga gacacaccac gcgtggttga
     5581 aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga cgggtgccct
     5641 ttcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccgacac tgtacaaact
     5701 ggcaggcgaa ctcacaccgt ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc
     5761 actctctatt tttggcgatc attccgacgt catggcggtg cgccagacgg gttgcgcgat
     5821 gttgtgtgcg gcaaacgtcc aggaagcgca agactttgct ctcatttcgc acatcgcgac
     5881 gctgaaaagc cgcgtgccat ttattcattt ctttgatggt ttccgcacgt cccacgaaat
     5941 caataaaatt gtcccgctgg ccgatgacac gattcttgat ctcatgccgc aggccgaaat
     6001 tgatgctcat cgcgcccggg cgctcaaccc ggaacatccg gtgatccgtg gtacgtccgc
     6061 caatcctgac tcttatttcc agtctcgcga agccaccaac ccctggtaca acgcagtcta
     6121 tgaccatgtt gaacaggcga tgaatgattt ctctgccgcg acaggtcgtc agtatcagcc
     6181 gtttgaatat tacgggcatc cgcaagctga acgggtgatt atcctgatgg gctcagccat
     6241 tggcacctgt gaagaagtgg ttgatgaatt gctaacccgt ggcgaaaaag tcggcgtgct
     6301 gaaagttcgc ctgtaccgcc ccttctcagc caaacacttg ctacaaactc taccgggatc
     6361 cgtacgcagc gtggcggtac tggacagaac caaagaaccc ggtgcccagg cagaaccgct
     6421 ctatctggat gtgatgaccg cactggcaga agcctttaat aatggcaagc gcgaaactct
     6481 gccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc cggactgtgt
     6541 actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta cggttggtat
     6601 ttacgatgat gtaaccaatc tgtccctgcc gttgccggaa aacacccagc caaactcggc
     6661 gaaacttgaa gccttgtttt atggccttgg tagtgatggc agcgtttccg cgaccaaaaa
     6721 caatatcaag attatcggta attccacgcc gtggtacgca cagggctatt ttgtttacga
     6781 ctccaaaaaa gcgggcggcc tgacggtttc tcaccttcgc gtgagcgaac agccgattcg
     6841 ttccgcttat ctcatttccc aggctgattt tgttggctgc caccagttgc agtttatcga
     6901 taaatatcag atggctgagc gtttaaaacc tggcggcatt ttcctgctca acacgccgta
     6961 cagcgcagat gaagtgtggt cgcgcttgcc gcaagaagtt caggccgtgt taaaccagaa
     7021 aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc
     7081 ccgtattaat accgtcatgc agatggcttt tttccatctg acgcaaattc tgcctggcga
     7141 tagcgccctc gcagaattgc agggtgcgat tgccaaaagt tacagtagca aaggccagga
     7201 tctggtggaa ggcaactggc aggctctggc gctggcgcgt gaatccgtag aagaagttcc
     7261 gttgcaaccg gtaaatccgc acagcgccaa tcgaccgcca gtggtttccg atgccgcccc
     7321 tgatttcgtg aaaaccgtaa ccgcagcgat gctcgcaggg cttggtgatg ccctccccgt
     7381 ttcggcgctg ccgccagacg gcacctggcc gatggggacc actcgctggg aaaaacgcaa
     7441 tatcgccgaa gagatcccca tctggaaaga ggaactctgt acccaatgta accactgcgt
     7501 cgccgcttgc ccacactcag ctattcgcgc aaaagtggta ccacccgaag cgatggaaaa
     7561 cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg ggcagaaata
     7621 tgtcttgcag gtggctccgg aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc
     7681 ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtcgc gcctggaaca
     7741 tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctgccagaaa tcgaccgtag
     7801 caaactggaa cgtattgata ttcgtacatc gcagctgatt acaccgctgt ttgaatattc
     7861 cggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc agctctatgg
     7921 cgaccggatg ttgatcgcta acgccaccgg ctgttcttca atttatggcg gtaacctgcc
     7981 ctctacaccg tataccaccg atgccaacgg tcgtgggccg gcatgggcga actctctatt
     8041 tgaagataat gccgaatttg gccttggttt ccgcctgacg gtcgatcaac accgtgtccg
     8101 cgtgctgcgt ctgctggatc aatttgccga taaaatcccg gcggaattac tgacggcgtt
     8161 gaaatcagac gctacgccag aggttcgtcg agaacaggtc gcagctttac gccagcaact
     8221 caacgatgtt gccgaagcac atgaactgct acgtgatgca gatgcactgg tggaaaaatc
     8281 aatctggctg attggtggtg atggctgggc ttacgatatc ggctttggcg gtctggatca
     8341 tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat gctattccaa
     8401 caccggtggt caggcgtcga aagcgacacc gctgggtgca gtaactaaat ttggcgagca
     8461 cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg gtcatgttta
     8521 tgtggcgcag atttctctcg gcgcgcagct gaatcagacg gtgaaagcga ttcaggaagc
     8581 ggaagcgtat ccgggaccat cgctgatcat tgcttatagc ccgtgtgaag agcatggtta
     8641 cgatctggca ctcagccatg accagatgcg ccaactcaca gctaccggct tctggccgct
     8701 atatcgcttc gatcctcgtc gtgccgatga aggcaaactg ccgctggcgc tggattcacg
     8761 cccgccgtca gaagcactgg aagaaacgtt acttcacgag caacgtttcc gtcgtctgaa
     8821 ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg atttgcaaaa
     8881 acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat
     8941 gctctggata aggattatcc aattctaaaa aaaagcccgg acgactgttc gggcttgtct
     9001 ttttatatgt tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac
     9061 aataaaggca tataacccgc gcagaataac gtatagaaaa ttaaaagtat aatttttatt
     9121 ttgtatgtat tatcttcgat acattcacca gacttattat catttcagaa gaattatttg
     9181 attaaggttt tacttaaggc gtaacaaatg atatttatcg gctaactgaa cttctccttt
     9241 aggatgtttt cactccccgg aatggggata aaaggcaaat aaaataacta aaggatttat
     9301 tcaatgaaaa gcaaagtact ggcactttta attcctgccc tgctcgccgc aggtgctgca
     9361 catgcagccg aagtttataa taaagacggc aacaaattag atctgtatgg caaagttgat
     9421 ggcctgcatt atttttctga taattcagcg aaagatggcg atcagagcta tgcgcgtctg
     9481 ggttttaaag gcgaaaccca aattaacgat caactcactg gctacggtca atgggaatac
     9541 aatattcaag caaacaacac tgaatcttca aaaaaccagt catggacccg tctggcattt
     9601 gccgggctga aatttgcaga ttacggttct ttcgattacg gacgtaatta tggcgtaatg
     9661 tacgacatcg aaggctggac cgatatgctg cctgaatttg gcggtgactc ttataccaat
     9721 gcagacaact ttatgactgg tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc
     9781 ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc
     9841 agtaatggtc aggaaggcac caacaacgga cgtgatgttc gccatgaaaa cggtgacggc
     9901 tggggttttt ccacaacata tgatttaggc atgggcttta gcgctggtac ggcatacacc
     9961 tcttctgacc gcaccaatga ccaggttaac catactgcgg cgggtggtga taaagcagac
    10021 gcgtggactg ctgggctaaa atacgatgct aacaatattt acctggcaac catgtattca
    10081 gaaacgcgta atatgacccc gtttggcgac agcgattatg ctgtggcaaa caaaacccag
    10141 aattttgaag tcactgccca gtaccagttt gattttggtc tgcgtccggc agtctctttc
    10201 ctgatgtcta aaggccgtga cctgcacgct gccggtggtg cagacaaccc ggcaggtgtt
    10261 gatgataaag atctggttaa atacgccgat gttggcgcga cttactattt caataaaaac
    10321 atgtccacct acgttgacta taaaatcaac ctgttggatg aagatgacag cttctacgct
    10381 gccaatggca tctctaccga tgatattgtc gctttaggtc tggtttatca gttctaaatc
    10441 ctcctgcccg ctgttatggc gggctttttc tgcttattct tcctcctttg atctaaatta
    10501 aaaatgtgaa ctccatcatt acacaaaaag tgtcatctgg cgttacactt tatgcggata
    10561 ctaaaacagg aggttttatg aacagaacga ttcttgtccc tatcgatatt tccgattcag
    10621 aattaactca acgcgtgatt agccacgttg aggcagaggc aaagattgac gatgcagagg
    10681 ttcatttcct gacggtaata ccttcactgc cctactatgc ctctctgggt ttagcgtatt
    10741 ccgcagaatt accggcaatg gatgacctga aagcggaagc caaatcgcaa ctggaagaga
    10801 tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt ccatgttgag gaaggctcgc
    10861 ccaaagaccg cattctggaa ttggcgaaga agatccccgc tcatatgatc atcattgctt
    10921 cccatcgacc ggatatcacc acttatctgc tcggttccaa cgccgcagct gtagtgcgtc
    10981 acgcagagtg ctccgtgctg gttgtgcgct gacactaacg cccgcacatt gctgcgggct
    11041 ttttgattca tttcgcaaat gtgctgacat tttcccctct aatccgtacc atacacgcca
    11101 ctgtttttat atcagatttc ttatgctggg cattccggca tgacgcatac tcttctgatg
    11161 ccatataacg aattgagtcg cttttaaatg tcgcaaaatc aagaaattag taagaaagaa
    11221 caatacaacc tgaacaagta agggcaaaaa tcacaactat ctgactcata agtattttat
    11281 ttatttttca atatgttaaa cattatcagc gacaacaata agctacgata acccactctt
    11341 tttttgcccc atttttgcca tcaacaattt tcctcataaa gtcatttcaa agcctaatag
    11401 ctgctctact tttttcctca atatcatgtc gccagcagga aacaacctaa tccctgtttt
    11461 aggcctcaaa gctgttaatg ataattattt tcattttgta taggttatgc tataaaacat
    11521 agccgttgct atgtttgttc taattttgct cactataggt actaaattat gcactcaata
    11581 aaaaaagtaa ccatgctctt ggggtgcctc gccctcacct gctcgatcgc atttcaggca
    11641 agtgcaactg aaaaattcaa ggtcattaca acatttacca tcatcgcgga tatggccaaa
    11701 aacgtggctg gagatgctgc agaagtctca tccataacca agcctggtgc agaaattcat
    11761 gagtatcagc ctacccctgg cgatattaaa cgtgcacagg gggcacaact gattctcgcc
    11821 aatggtatga atctggaatt gtggttccaa cgcttttacc agcatctcaa tggggttcca
    11881 gaagtaattg tctcttcggg tgtgacgcca gtagggatca ccgaaggacc ctatgagggc
    11941 aaacctaatc cccatgcatg gatgtcgcca gataatgctc tgatttacgt cgataatatt
    12001 cgtgatgcgt tgataaaata cgatccggca aatgcacaaa cctaccaacg taatgccgat
    12061 acttataaag ccaagattac ccaaaccctt gcccccctgc gtaagcagat tacggaactc
    12121 cctgagaatc agcgatggat ggtcaccagt gaaggggctt tttcttatct cgcacgcgat
    12181 ttggggctaa aagagcttta tctgtggccg attaatgccg atcaacaagg aacaccgcag
    12241 caggtacgta aggttgttga tatagttaag aaaaatcata tcccggcagt ctttagcgag
    12301 agtacgattt ccgataaacc agcgcgtcag gttgcgcgtg aaaccggcgc acactacggc
    12361 ggtgtgctct atgtcgattc cctgagcaca gaaaacggcc cggtacctac gtatatcgac
    12421 cttcttaagg ttactaccag tacgctggta cagggaatta aagccgggaa aagggagaaa
    12481 taatgatgca atctgcaggc attgtcgtta atgatgttac tgtcacctgg cgtaacgggc
    12541 acacagcact gcgcgatgca tccttcacag taccaagtgg atctattgcc gctctggttg
    12601 gggtaaacgg ttccggaaaa tcgacgttgt ttaaagcgat tatgggattt gtgcgtctga
    12661 ccagcggaaa gatatctgtt ctgggtattc ccacgcgaca ggcgctacag aaaaacctgg
    12721 ttgcctacgt ccctcagtca gaagaggttg actggtcatt tcctgtactg gtggaagatg
    12781 tggtgatgat gggccgctat gggtatatgg gtatgttgcg tatagccaaa aagagggatc
    12841 gtcagattgt caccgatgcg ctggaacgtg tcgatatggt ggattttcgc catcgtcaaa
    12901 tcggcgagct ttccggtgga caaaagaaac gcgtctttct ggcgagagcg attgcacagc
    12961 agggtgatgt gatcctgctg gatgaacctt ttaccggcgt tgatgtgaaa accgaagcga
    13021 aaatcatcag cctgttgagg gagttacgcg cggaaggcaa aacgatgctt gtctcaacgc
    13081 acaatctcgg ctcagtcacg acattttgcg attatacggt catggtcaaa ggcaccgtcc
    13141 tggcaagcgg gccgacagac accactttta cagccgagaa cctggagctg gcttttagcg
    13201 gcgtactgcg ccatgtcaca cttaacggct cggaagaaag cattattact gacgatgaac
    13261 gcccttttgt ggcacatcga ccgtcagcgg tgcagaggga agcaagatga acgtgctact
    13321 ggaacccttc agctatgaat atatgctcaa tgcgatgtgg gtctcggcaa tggtcggcgg
    13381 tctctgcgct ttcctgtcat gctatctgat gctaaaaggc tggtcgctga ttggcgatgc
    13441 gctgtcgcac tctattgtcc cgggtgttgc gggggcatat atgctggggc ttccgttttc
    13501 tctgggagcc ttcttttccg gcggactggc agcgggcagc atgctgtttc ttaaccaacg
    13561 cacccgctta aaagaagatg ccatcatcgg cctgatcttc tcgtcctttt ttggcctggg
    13621 gctgtttatg gtgtcattga acccgacatc cgtgaacatt cagaccatcg tcctgggcaa
    13681 tattctggca atcgatccgg cggatatcct gcaactcact atcattggca ttctctcgat
    13741 catcgtgctt tttttcaaat ggaaagatct tatggtgacc tttttcgacg agaatcacgc
    13801 ccgtgctatt ggattacatc cgggcagatt aaaaatcctc ttcttcaccc tgctgtcggt
    13861 ttcgaccgtt gcggcgctgc aaacggttgg tgcctttctg gtgatctgtc tggtggtgac
    13921 tcccggtgca acagcatggt tgttaaccga tcgttttccc cgcctgctca taattgctgt
    13981 aactattggc agtgtaacca gtttcctcgg cgcgtgggtc agttactttc tggacggcgc
    14041 gacaggtgga atcatcgtgg tggcgcagac actgctgttt cttttagcat ttgtatttgc
    14101 gcccacccat ggacttttgg caaatcgtcg gcgtgcgcat aaagcgctgg aggatcgctc
    14161 atgatggcat tattactgga gccactgcag ttcaccttta tgagtcacgc actgctgatt
    14221 tcactggtag tttccatacc ctgtgcgctg ctgtcggtct ttctggtcct gaaaggttgg
    14281 gcactgatgg gtgacgcgat gagtcatgcg gtttttcctg gcatcgtgct tacctggatt
    14341 ctggggttac ctctggctac aggggcattc gtcgccggag tattctgcgc ggtggcgacg
    14401 ggatacctga aagacaacag ccgaatcaag caagatacag ttatggggat tgttttttct
    14461 ggcatgtttg ccgcaggtct gatcttgtat attgcaatca aaccagacgt acatcttgac
    14521 catattcttt tcggcgacat gctcgggata accatcggcg atataatcca gacggtgatt
    14581 attgccgggc tggttacact tgttattagc gtaaaatggc gggatttttt gctgttcagc
    14641 tttgattatc aacaggcgca ggtaagcggt ttgcatacga gatggctgca ttatgggctg
    14701 ctgtgcatgg tttccctgac cattgtggcg acgctgaaag cagtaggtat cattctctct
    14761 atttctctgc tcattgcgcc tggcgccatt gcagtactcc tgacacaacg ctttcatatt
    14821 gcgctcctac tggcgactgg catatcggta atagtgtcaa tgactggtgt ctggctgtcc
    14881 ttttttatcg acagcgcacc agctccgacg attgtcgtct tattcgcggt cgtgttcatc
    14941 atgacgttta ccgttaccag catcaacgca cgcacaaagg gaaacgccca tacacaggat
    15001 ctgttatcac ccaattagca caatatccga ggacagcatc gtccgccagc ctacagaagt
    15061 tcggaaagcc atggaaggtc gttgggagga ggtacgaatt ctatcatgca aaaaatacgt
    15121 aaaatcgata acgcctagaa atcattcaat actcgcacta tcgaaagttt accagccaac
    15181 cgcggcacgt tcctgcatac gacgtgccgc agcttctgtt attccacgat aaaattccta
    15241 accgaattca aatccttcag actattcagc tcttccttca gctcccgctg acggcgataa
    15301 at
//
LOCUS       DADLTD010000094        15063 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLTD010000094 DADLTD010000000
VERSION     DADLTD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.094"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            49..993
                     /locus_tag="JN701_002297"
     CDS             49..993
                     /locus_tag="JN701_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7364736.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1035..1472)
                     /locus_tag="JN701_002298"
     CDS             complement(1035..1472)
                     /locus_tag="JN701_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY7364737.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1518..1859)
                     /locus_tag="JN701_002299"
     CDS             complement(1518..1859)
                     /locus_tag="JN701_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364738.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2024..2215)
                     /locus_tag="JN701_002300"
     CDS             complement(2024..2215)
                     /locus_tag="JN701_002300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364739.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2148..3476)
                     /gene="xylB"
                     /locus_tag="JN701_002301"
     CDS             complement(2148..3476)
                     /gene="xylB"
                     /locus_tag="JN701_002301"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY7364740.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3593..4870)
                     /gene="xylA"
                     /locus_tag="JN701_002302"
     CDS             complement(3593..4870)
                     /gene="xylA"
                     /locus_tag="JN701_002302"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAY7364741.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5236..6207
                     /gene="xylF"
                     /locus_tag="JN701_002303"
     CDS             5236..6207
                     /gene="xylF"
                     /locus_tag="JN701_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7364742.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6285..7826
                     /gene="xylG"
                     /locus_tag="JN701_002304"
     CDS             6285..7826
                     /gene="xylG"
                     /locus_tag="JN701_002304"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY7364743.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7804..8985
                     /gene="xylH"
                     /locus_tag="JN701_002305"
     CDS             7804..8985
                     /gene="xylH"
                     /locus_tag="JN701_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY7364744.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            9063..10218
                     /locus_tag="JN701_002306"
                     /pseudo
     CDS             9063..10218
                     /locus_tag="JN701_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(10326..11150)
                     /locus_tag="JN701_002307"
     CDS             complement(10326..11150)
                     /locus_tag="JN701_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY7364745.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11470..13500
                     /gene="malS"
                     /locus_tag="JN701_002308"
     CDS             11470..13500
                     /gene="malS"
                     /locus_tag="JN701_002308"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7364746.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            13678..14931
                     /gene="avtA"
                     /locus_tag="JN701_002309"
     CDS             13678..14931
                     /gene="avtA"
                     /locus_tag="JN701_002309"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY7364747.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
BASE COUNT         3892 a         3740 c         3641 g         3790 t
ORIGIN      
        1 cagcccaaaa tttactggat tgataacctg cgagggatag cgtgtttaat ggtggtgatg
       61 attcacacca ctacctggta tgtgaccaat gctcatagtg ttagccccgt cacatgggat
      121 atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc cgctattttt catgatttcc
      181 ggctatctct tttttggcga acgcagcgcc cagccgcgcc atttcttgcg tatcggctta
      241 tgtctgattt tttatagcgc aatcgcactg ctctacattg cgctgtttac ctccatcaat
      301 atggagttag cgctgaaaaa cctgctgcaa aagccagtgt tttaccactt gtggtttttc
      361 ttcgcgattg cggtgattta tctggtttca ccgctgattc aggtgaagaa cgtcggcgga
      421 aaaatgttgc tggtgctaat ggcggtgatt ggcattatcg ctaacccaaa cacagtgccg
      481 cagaaaattg acggttttga atggctgcca attaacttat atatcaatgg cgatactttt
      541 tactacattc tgtatggcat gttgggccgc gctataggga tgatggacac acagcataaa
      601 gcactgtcgt gggtgagcgc cgcgctgttt gcgacggggg tttttattat ctctcgcggg
      661 acattatatg aattgcagtg gcgcggaaat tttgccgata cctggtatct ttactgtggg
      721 ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg ttaaaaacac gctggatacg
      781 cgtaccattc gcggacttgg cttaatctcc cgccattcat tgggtatata cggattccac
      841 gccttgatta tccatgcgct gcgcacccgg ggaattgagc ttaaaaattg gccaatactg
      901 gatattattt ggatcttttg cgcgacgttg gcagcgagtt tgttactttc tatgctggta
      961 caacgaatcg acagaaacag attagtgagt taagtaaaag cccgttcaca ttggactgac
     1021 cgggcttacg tgaattattc gctgtattct tcctgggtaa cctgtgtttc ttttggcggg
     1081 tttattccgg catcacgaat attcttctgc accgctactg caccaaacaa gcttaagaag
     1141 aaagccagtc cataaaaacc tttttcactg agtaataatg tcgcattcca cagacctacc
     1201 atcagtaatg caacagagat aataaagaca gtcaggcagg tcatataata aatggaagtg
     1261 gtcggtatgc cttcatactt gtcccgaacg gtcttttgat aagacgcagc agaaaacagt
     1321 cctaacacca gtacggcaaa ataataccct ttttcattta actgcatctc tgcattccat
     1381 agccctaaca gataggtaac gataccacca acgagagcta tccatgacac aatactaaag
     1441 gccggtgaat aggttgatat tttgttgtcc atcacagtat tcctttcatt cctgaatgtg
     1501 taagagcttt atgttgctca taccgagcga aacaaacgca tttgaccaaa caagcagaca
     1561 aaaaaggcga tggggtagat caccatttca taagcattga taggtgcatt taaaacaccg
     1621 ataaacaaga gaccgatagt gattaacgca actaactggc agatatgggt aaaaaaatta
     1681 tccagttgcc cgagtttttc tgcgcgaaga tatgcatagt taccaaaagt tgctgtcatt
     1741 aacacgccaa gaaaatagcc ttttccactt agcaatgggc atgagatcca tagcccaacc
     1801 agataaacca gcgccccgac aacaaataat agttttgcca cagtttttga ggttttcata
     1861 acgatctcca tatctaccag cgatacatta cgagtaacca acgaaagaca aaactgaaaa
     1921 atgccattaa caaatgattt tcagaataaa ttcatactaa atattaatta gtgagatata
     1981 tagatgtgaa ttatccccca cccggtcagg caggggataa cgtttacgcc attaatggca
     2041 gaagttgctg atagaggcga cggaacgttt cacgtcgtgg ctgataagcg gcataacgct
     2101 gcgcatctgg tagatgcgac tgttctaacg gtagctgcgg caacaactca atgagcgatt
     2161 tctctggatt cgccgcaatc tgcgccagcc ttgctgcgcc cagtgctggc cccacatccc
     2221 cccgtacggt aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca
     2281 ctacgcgccc cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg
     2341 acatccatgc catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca
     2401 ttggggccat gttgatgagt caaaccaaag aaaaccccct tcgcctgggg attattgtgt
     2461 ggtgtacgct cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc
     2521 tgttgagctg cagcgattaa agctgggaca ttgcacaggc cggttaattt cgcgacccaa
     2581 tccagacacg atgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca
     2641 tggcaaaagc tgtgtacagc gctttctggc ttgcttaaga acccttcgct gacagcaaaa
     2701 tagacccccg acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca
     2761 actgcaccag ctgcattgtc gccaccgcct gcgacaactg gcaccgtcgc cataccccac
     2821 gctttcgcaa cttcaggtaa caaagcacca gtaatttcgc tgccttcgta taatgcgggc
     2881 atctggtcac gagataagtc gcaagcctgc agcatgacgt cactccagtc acgctttgcg
     2941 acatccagcc acatggtgcc agctgcgtca gacatatcgc tggcaaactc ccccgtcata
     3001 cgcagacgca agtaatcttt cggtaataat actttgtcga tttgacggaa tatctccggc
     3061 tcatgccgct gaacccatag caatttaggc gcagtaaatc cgggcatcat caggttgccg
     3121 gtaatcactc gtgattgcgg aactctcgct tccagcaaag tgcactcttg cgcacagcgc
     3181 ccgtcgttcc acaaaatggc agggcgcaat acccgttgtt gagcatccag taaggttgct
     3241 ccatgcatct ggccggcaat acccaatgct ttaacgtcct gcagagaatg ctgatcgccc
     3301 agagctttca ttgcgcgatc agttgcctgc caccactgtt ccgggtcttg ttccgaccag
     3361 agtggatgcg ggcgcgaaac ggtcagcttt tccgtttgcg aagcaaccac ctcaccctgc
     3421 tcgttgagca aaataacttt tacgcccgag gtgccaagat ctatcccgat atacatatcg
     3481 atcgttcctt aaaaaaatgc ccggtatcgc taccgataac cgggccaacg gactgcacag
     3541 ttagccgtta tttgtcgaac agataatgat ttaccagatt ttccagttgc tcctagcgac
     3601 cactctgatg caccggagac aaattatgtt cctgagcata tttggctaaa tctgccagtg
     3661 acatttggcc tttcaggatt tgctggccca attcgctatt ccagccggaa taacgctgcg
     3721 cgatgcgttt atccagctcg ccatcttcaa tcatgcgcgc tgcaattttc agcgccagtg
     3781 ccatcgtatc catcgcgccg atatgaccgt aaaacagatc atatttatca gtactttgac
     3841 gacgtacttt ggcatcgaag ttcagaccac cggtggtgaa accgcctgct ttgagaattt
     3901 catacatcac cagcgcattc tcttccacac tgttcgggaa ctggtcggtg tcccagccca
     3961 gttgcgcatc gccacggttg gcgtcgacag aaccgaacag gccaagcgca atggcggtgg
     4021 ctatttcatg atggaaagag tgacctgcca gcgtcgcgtg gttagcttca atgttcagtt
     4081 taatctcttt ttccagacca aactgtttca ggaagccata gaccgtcgcg gcatcgtaat
     4141 catattgatg tttagtcggt tcttgcggtt tcggttcgat aagcaacgtg ccctggaagc
     4201 cgattttatg tttatgctca accaccatct gcataaagcg gcccagttgt tcacgctcct
     4261 gacgcaagtc ggtatttaac agcgtttcgt aaccttcacg accaccccac aggacatagt
     4321 tttcaccgcc caatttatgg gttgcttcca tcgctgtaac aacttgcgtt gccgcccagc
     4381 tgaagacttc aggatctggg ttcgtcgccg cacccgcgcc atagcgaggg tttgtaaagc
     4441 agttagcggt tccccacagc agcttcacgc cgctctcttc ttgcttggct gccaggacat
     4501 caaccatttg cgcaaaatta ttgatgtact cttttaacga cgcgccctca ggggaaacat
     4561 ccacatcgtg gaagcaataa aatgacacat gtaacttgtg gaaaaactca aatgcgacat
     4621 ctgctttacg cttcgccaac gccagtgcct caccaggctg ctgccacgga cgattaaacg
     4681 cccccacacc aaacatgtcc gccccgttcc agcagaaggt gtgccagtaa caggcggcaa
     4741 aacgcaagtg ctcttccata cgcttaccca acaccagttc gtcgggattg tagtgacgga
     4801 atgctaacgg gtttgaggat tttgagcctt cataacgaac gcgatcgagc tggtcaaaat
     4861 aggcttgcat attgaactcc ataatcaggt aatgccgcgg gtgatggatg atgtcgtaat
     4921 attgggcact ccctttcagt tgctcaatta tgttatttca cactgctatt gagataattc
     4981 acaagtgtgc gctcgctcgc aaaataaaat gggatgatga aactgggtaa ttcctcgaag
     5041 ataaaaatgc aataagtaca attgcgcaac aaaagtaaga tctcggtcat aaatcaagaa
     5101 ataaaccaaa aatcgtaatc gaaagataaa aatctgtaat tgttttcccc tgtttagttg
     5161 ctaaaaattg gttacgttta tcgcggtgat tgttacttat taaagctgtc ctctaactac
     5221 agaaggccct acaccatgaa aataaagaac attctactca ccctttgcac ctcacttctg
     5281 cttaccaacg ttgcggcaca cgccaaagaa gtcaaaatag gtatggcgat tgatgatcta
     5341 cgtcttgaac gctggcaaaa agatcgagat atttttgtga aaaaggctga atctctcggt
     5401 gcgaaagtat ttgtacagtc tgcaaatggc aatggagaaa cacaaatgtc gcagattgaa
     5461 aacatgatta accggggtgt cgatgttctt gtcattattc cgtataacgg ccaggtatta
     5521 agtaacgttg taaaagaagc caaacaagaa ggtattaaag tattagctta cgaccgtatg
     5581 attaacgatg cggatatcga tttttatatt tctttcgata acgaaaaagt cggtgaactg
     5641 caggcaaaag ccctggtcga tattgttccg caaggtaatt acttcctgat gggcggctcg
     5701 ccggtagata acaacgccaa gctgttccgc gccggacaaa tgaaagtgtt aaaaccttac
     5761 gttgattccg gaaaaattaa agtcgttggt gaccaatggg ttgatggctg gttaccggaa
     5821 aacgcgctaa ccgccaataa taacaaaatt gatgctgtag ttgcctcaaa cgatgccacc
     5881 gcaggtgggg caattcaggc attaagcgcg caaggtttat cagggaaagt agcaatttcc
     5941 ggccaggatg cggatctcgc aggtattaaa cgtattgctg ccggtacgca aactatgacg
     6001 gtgtataaac ctattacatt gctggcaaat actgccgcag aaattgccgt tgaattgggc
     6061 aatggtcagg agccaaaagc agatacctca ctgaataatg gcctgaaaga tgtcccctcc
     6121 cgcctcctga caccgatcga tgtgaataaa aacaacatca aagatacggt aattaaagac
     6181 ggattccaca aagagagcga gctgtaagcg ttacgccccg gcgcggagcg ggggcgtgat
     6241 ttctctccat gccgcgtgaa tgaattggct taggtggagt cgttatgcct tatctacttg
     6301 aaatgaagaa cattaccaaa accttcggca gtgtgaaggc gattgataac gtcagcttgc
     6361 ggttgaatgc tggcgaaatc gtctcactct gtggggaaaa tgggtctggt aaatcaacgc
     6421 tgatgaaagt gctgtgtggt atttatcccc atggctccta cgaaggcgaa attatttttg
     6481 cgggagaaga gattcaggcg agtcacatcc gcgataccga acgcaaaggt atcgccatca
     6541 ttcaccagga attggccctg gtgaaagaat tgaccgtgct ggaaaatatc ttcctgggta
     6601 acgaaataac ccacaatggc attatggatt atgacctgat gacgctacgc tgtcagaagc
     6661 tgctcgcaca ggtcagttta tccatttcac ctgatacccg cgttggcgat ttagggcttg
     6721 ggcaacaaca actggttgaa attgccaagg cacttaataa acaggtgcgc ttgttaattc
     6781 tcgatgaacc gacagcctca ttaactgagc aggaaacgtc ggttttactg gatattattc
     6841 gcgatctaca acagcacggt atcgcctgta tttatatttc gcacaaactc aacgaagtca
     6901 aagcgatttc cgatacgatt tgcgttattc gcgacggtca gcacattggt acgcgtgatg
     6961 ctgccggaat gagtgaagac gatattatca ccatgatggt cgggcgagag ttaaccgcgc
     7021 tttaccctaa tgaaccacat accaccggag atgaaatatt acgtattgaa catctgacgg
     7081 catggcatcc ggttaatcgt catattaaac gagttaatga tgtctcgttt tccctgaaac
     7141 gtggcgaaat actgggtatt gccggactcg ttggtgccgg acgtaccgag accattcagt
     7201 gcctgtttgg cgtgtggccc ggacaatggg aaggaaaaat ttatattgat ggcaaacagg
     7261 tagatattcg taactgtcag caagccatcg cccaggggat tgcgatggta cccgaagaca
     7321 gaaagcgcga cggcatcgtt ccggtaatgg cggttggtaa aaatattacc ctcgccgcac
     7381 tcaataaatt taccggtggc attagccagc ttgatgacgc ggcagagcaa aaatgtattc
     7441 tggaatcaat ccagcaactc aaagttaaaa cgtcgtcccc cgatcttgct attggacgtt
     7501 tgagcggcgg caatcagcaa aaagcgatcc tcgcccgctg tctgttactc aacccgcgca
     7561 ttctcattct tgatgaacct accaggggta ttgatattgg cgcgaaatat gagatctaca
     7621 aattaattaa ccaactcgtc cagcagggta ttgccgttat tgtcatctct tccgaattac
     7681 ctgaagtgct cggccttagc gatcgtgtac tggtgatgca tgaagggaaa ctaaaagcca
     7741 acctgataaa tcataacctg actcaggagc aggtgatgga agccgcattg aggagcgaac
     7801 atcatgtcga aaagcaatcc gtctgaagtg aaattggccg taccgacatc cggtggcttt
     7861 tccgggctga aatcactgaa tttgcaggtc ttcgtgatga ttgcagctat catcgcaatc
     7921 atgctgttct ttacctggac caccgatggt gcctacttaa gcgcccgtaa cgtctccaac
     7981 ctgttacgcc agaccgcgat taccggcatc ctcgcggtag gaatggtgtt cgtcataatt
     8041 tctgctgaaa tcgacctttc cgtcggctca atgatggggc tattaggtgg cgtcgcggcg
     8101 atttgtgacg tctggttagg ctggcctttg ccacttacca tcattgtgac gttggttctg
     8161 ggactgcttc tcggtgcgtg gaacggatgg tgggtcgcgt accgtaaagt cccttcattt
     8221 attgtcaccc tcgcgggcat gttggcattt cgcggcatac tcattggcat caccaacggc
     8281 acgactgtat cccccaccag cgccgcgatg tcacaaattg ggcaaagcta tctccccgcc
     8341 agtaccggct tcatcattgg cgcgcttggc ttaatggctt ttgttggttg gcaatggcgc
     8401 ggaagaatgc gccgtcaggc tttgggttta cagtctccgg cctctaccgc agtagtcggt
     8461 cgccaggctt taaccgctat catcgtatta ggcgcaatct ggctgttgaa tgattaccgt
     8521 ggcgttccca ctcctgttct gctgctgacg ttgctgttac tcggcggaat gtttatggca
     8581 acgcggacgg catttggacg acgcatttat gccatcggcg gcaatctgga agcagcacgt
     8641 ctctccggga ttaacgttga acgcaccaaa cttgccgtgt tcgcgattaa cggattaatg
     8701 gtagccatcg ccggattaat ccttagttct cgacttggcg ctggttcacc ttctgcggga
     8761 aatatcgccg aactggacgc tattgcagca tgcgtgattg gcggcaccag cctggctggc
     8821 ggtgtgggaa gcgttgccgg agcagtaatg ggggcattta tcatggcttc actggataac
     8881 ggcatgagta tgatggatgt accgaccttc tggcagtata tcgttaaagg tgcgattctg
     8941 ttgctggcag tatggatgga ctccgcaacc aaacgccgtt cttgattttg ataaaaattt
     9001 tctcaaagcc ggttacgtat taccggtttt gagtttttgc atgattcagc aggaaaagaa
     9061 ccatgtttac taaacgtcac cgcatcacat tactgttcaa tgccaataaa gcctatgacc
     9121 ggcaggtagt agaaggcgta ggggaatatt tacaggcgtc acaatcagaa tgggatattt
     9181 tcattgaaga agatttccgc gcccgcattg ataaaatcaa ggactggtta ggagatggcg
     9241 tcattgccga cttcgacgac aaacaaatcg agcaagcgct ggctgatgtc gacgtcccca
     9301 ttgttggggt tggcggctcg tatcaccttg cagaaagtta cccacccgtt cattacattg
     9361 ccaccgataa ctatgcgctg gttgaaagcg catttttgca tttaaaagag aaaggcgtta
     9421 accgctttgc tttttatggt cttccggaat caagcggcaa acgttgggcc actgaacgcg
     9481 aatatgcatt tcgtcagctt gtcgccgaag aaaagtatcg cggagtggtt tatcaggggt
     9541 tagaaaccgc gccagagaac tggcaacacg cgcaaaatcg gctggcagac tggctacaaa
     9601 cgctgccacc gcaaaccggg attattgccg ttactgacgc ccgggcgcgg catattctgc
     9661 aagtatgtga acatctacac attcccgtac cggaaaaatt atgcgtgatt ggcatcgaca
     9721 acgaagaact gacccgctat ctgtcgcgtg tcgccctttc ttcggtcgct cagggcgcgc
     9781 ggcaaatggg ctatcaggcg gcaaaactgt tgcatcgatt attagataaa gaagaaatgc
     9841 cgctacagcg aattttggtc ccaccagttc gcgtcattga acggcgctca acagattatc
     9901 gctcgctgac cgatcccgcc gttattcagg ccatgcatta cattcgtaat cacgcctgta
     9961 aagggattaa agtggatcag gtactggatg cttgagaagc gttttaaaga agaggtgggt
    10021 gaaaccatcc atgccatgat tcatgccgag aagctggaga aagcgcgcag tctgctgatt
    10081 tcaaccacct tgtcgatcaa tgagatatcg caaatgtgcg gttatccatc gctgcaatat
    10141 ttctactctg tttttaaaaa agcatatgac acaacgccaa aagagtatcg cgatgtaaat
    10201 cgcgaggtca tgttgtagcg atgatgagaa ttgtcggcgt cacatcaggg aactcagcac
    10261 agccacctga tgcgagcaaa agccttatcc gaccgacaaa tcagataagg ctcaaggaaa
    10321 tgcaattaca tatgcgccgc gattaaccgt tggttatcct ggtacattgc gaacaggtag
    10381 ttattataac tcttcccttt ggtcgagtag cccttcagct tgtgaatcat cgctgtggca
    10441 gtcacttcct gatccgcttt acgcagctgc gcacgcgatt tacggaacga agagtaagcc
    10501 gggtgcgtat tcaggttagt gacataggcg ctcaccgatt ctttgacaga actaaactgt
    10561 gagtaccctt tcactttacc cggcgcattg gtacaacgtc ctttcatgca tttcatgccg
    10621 aacagattgt tattgttgcg cgccagcttc gacgttcccc aaccgctttc tgctgcagcc
    10681 atcgtcgcca ccatactggt agggataatg tctacgcgtt caagcaaggt attccacggg
    10741 atttttcgcg tattaccaga ccacttcacc ttgtagcgtt tggcgatgtc tttcagacgc
    10801 gcacgctcag caggtgacca ttggccctga tactgttttg aaatcagcca gttacgttct
    10861 gcagtaatgg ctgcattttg gctggtaatg taaggcatta cggtccggag aaacgccttt
    10921 ttccttggtg ttccggaagg gtattttcgc aaatcaggaa gtgaactact ctttgcacta
    10981 ttgcgagaat actcttgttt actgcttacc tgtttattac tagctttagt taagtgggac
    11041 ttttgactcg ctgttgttgt gtgcgtcttc gctaacacct cactcgaaaa caccagagtg
    11101 agtaacataa gaatcatcgc cccatatcgt cgtatgggag tcaaaatcat caggtctcct
    11161 ggtcggattt aatcattcca acaccttata tttttcacaa atttgagagt tgaatctcaa
    11221 atcatatcaa aaatagctgt caagagcacc ccaaggaata gtccaaatct gaaactatgt
    11281 cacgagttaa cgattcagat tggcgctaaa tcgcagaaaa tgtggggttt atcgcaaaat
    11341 tcagccgttt tttgcgcgcg atcgctcacc cttgcttctc atcctgtgga cttaccgctc
    11401 agggatgagt tttgtctggc ttatcgctgg caaactgtct gaaatcgcag caataaggac
    11461 tcatccacca tgaaactcgc ctcctgtttt ctgacactcc ttcctggctt cgccgttgcc
    11521 gccagctgga cttctccggg gttccctgcc tttagcgaac agggaacggg aacatttgtc
    11581 agccacgcgc agttgcccaa aggtacgcgt ccactcacgc taaattttga ccaacagtgc
    11641 tggcagcctg cagatgcgat aaaactcaat cagatgcttt ccctgcaacc ttgtagcaac
    11701 acgccgcctc aatggcgatt gttcagggac ggcaaatata cgctgcaaat agacacccgc
    11761 tccggtacgc caacattgat gatttccatc cagaacgccg ccgaaccggt agcaaacctg
    11821 gtccgtgaat gcccgaaatg ggatggatta ccgctcacgc tggatgtcag cgccactttc
    11881 ccggaaggag ccgccgtacg ggattattac agccagcaaa ttgcgatagt gaagaacggt
    11941 caaataacgt tacaacccgc tgctaccagc aacggtttac tcctgctgga acgggcagaa
    12001 actgacacat ccgccccttt cgactggcat aacgccacgg tttactttgt gctgacagat
    12061 cgtttcgaaa acggcgatcc cagtaatgac cagagttacg gacgtcataa agacggtatg
    12121 gcggaaattg gcacttttca cggcggcgat ttacgcggcc tgatcaacaa actggattac
    12181 ctccagcagt tgggcgttaa tgctttatgg ataagcgccc catttgagca aattcacggc
    12241 tgggtcggcg gcggtacaaa aggcgatttc ccgcattatg cctaccacgg ttattacaca
    12301 caggactgga cgaatcttga tgccaatatg ggcaacgaag ccgatctacg gacgctggtt
    12361 gatagcgcac atcagcgcgg tattcgtatt ctctttgatg tcgtgatgaa ccacaccggc
    12421 tatgccacgc tggcggatat gcaggagtat cagtttggcg cgttatatct ttctggtgac
    12481 gaagtgaaaa aaacgctggg tgaacgctgg agcgactgga aacctgccgc cgggcaaacc
    12541 tggcatagct ttaacgatta cattaatttc agcgacaaaa caggctggga taaatggtgg
    12601 ggaaaaaact ggatcagaac ggatatcggc gattacgaca atcctggatt cgacgatctc
    12661 actatgtcgc tggccttttt gccggatatc aaaaccgaat caactaccgc ttctggtctg
    12721 ccggtgttct ataaaaacaa aacggatact cacgctaaag tcatcgaagg ctttacacct
    12781 cgcgattact taacccactg gttaagtcag tgggtccgcg actatgggat tgatggtttt
    12841 cgggtcgata ccgccaaaca tgttgagttg cccgcctggc agcaactgaa aaccgaagcc
    12901 agctccgcgc ttcgcgaatg gaaaaaaact aaccccgaca aagcattaga tgacaaacct
    12961 ttctggatga ccggtgaagc ctggggccac ggcgtgatgc aaagtgacta ctatcgccac
    13021 ggcttcgatg cgatgatcaa tttcgattat caggagcagg cggcgaaagc agtcgattgt
    13081 ctggcgcaga tggatacgac ctggcagcaa atggcggaga aattgcaggg tttcaacgtg
    13141 ttgagctacc tctcgtcgca tgatacccgt ctgttccgtg aagggggcga caaagcagca
    13201 gagttattac tattagcgcc aggcgcggta caaatctttt atggcgatga atcctcgcgt
    13261 ccgttcggtc ctacaggttc tgatccgctg caaggtacac gttcggatat gaactggcag
    13321 gatgttagcg gtaaatctgc cgccaacgtc gcgcactggc agaaaatcag ccagttccgc
    13381 gcccgccatc ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa gcagggctac
    13441 ggctttgttc gtgagcatgg cgacgataaa gtgctggtca tctgggctgg gcaacagtga
    13501 cttttccggc ttcccgttca tcagtacctc gggaagccgc caaccaggat aaaatgtcag
    13561 ccctaatcag cgttgcagga taaagcaccg ctcactcttc aacagaccga tttgcacccc
    13621 agcaaatgta gcgttattgt taccttcctt gctacagagt tcgacagata tcccgctatg
    13681 acattctccc tttttggtga caaatttacc cgccactccg gcattacgct gctgatggaa
    13741 gatctgaacg acggtttacg cacgcctggc gcgattatgc tcggcggcgg taatccggcg
    13801 cagatcccgg aaatgcagga ctacttccag acgctactga ccgacatgct ggaaagtggc
    13861 aaagcgactg atgcactgtg taactacgac ggtccacagg ggaaaacgga gctactcaca
    13921 ctgcttgccg gaatgctgcg cgagaagttg ggttgggata tcgaaccaca gaatattgca
    13981 ctaacaaacg gcagccagag cgcgtttttc tacttattta acctgtttgc cggacgccgt
    14041 gccgatggtc gggtcaaaaa agtgctgttc ccgctggcac cggaatacat tggctacgct
    14101 gacgccggac tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga actgctgccg
    14161 gaaggccagt ttaaatacca cgtcgatttt gagcatctgc atattggcga agaaaccggg
    14221 atgatttgcg tctcccggcc gacgaatcca acaggcaatg tgattactga cgaagagttg
    14281 ctgaagcttg acgcgctggc gaatcaacac ggcattccgc tggtgattga taacgcttat
    14341 ggcgtcccgt tcccgggtat catcttcagt gaagcgcgcc cgctatggaa tccgaatatc
    14401 gtgctgtgca tgagtctttc caagctgggg ctgcctggct cccgctgcgg cattatcatc
    14461 gccaatgaaa aaatcatcac tgccatcacc aatatgaacg gcattatcag cctggcacct
    14521 ggcggtattg gtccggcgat gatgtgtgaa atgattaagc gtaacgatct gctgcgcctg
    14581 tctgaaacag tcatcaaacc gttttactac cagcgtgttc aggaaactat cgccatcatt
    14641 cgccgctatt taccggaaaa tcgctgcctg attcataaac cggaaggagc cattttcctc
    14701 tggctatggt ttaaggattt gcccattacg accgagcagc tctatcagcg cctgaaagca
    14761 cgcggcgtgc tgatggtgcc ggggcacaac gtcttcccgg gactggataa accgtggccg
    14821 catacgcatc aatgtatgcg catgaactac gtaccagagc cggagaaaat tgaggcgggg
    14881 gtgaagattc tggcggaaga gatagaaaga gcctgggctg aaagtcacta aaattgcctg
    14941 atgcgctacg cttatcaggc ctacatgatt tctgcaatat attgaatttg gaagaatttg
    15001 taggccagat aaggcgttta cgccgcatct ggcataaaca aagcgcacta ttccggcgtt
    15061 gag
//
LOCUS       DADLTD010000095        14937 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLTD010000095 DADLTD010000000
VERSION     DADLTD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.095"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..450
                     /locus_tag="JN701_002310"
     CDS             <1..450
                     /locus_tag="JN701_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HAY7364748.1"
                     /translation="QEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIA
                     DPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMT
                     LGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(491..1423)
                     /locus_tag="JN701_002311"
     CDS             complement(491..1423)
                     /locus_tag="JN701_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7364749.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1543..1785)
                     /locus_tag="JN701_002312"
     CDS             complement(1543..1785)
                     /locus_tag="JN701_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364750.1"
                     /translation="MSVPVYQIMGKGCHRQDKNMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHVVKNKAIPNN"
     gene            1787..4573
                     /gene="polA"
                     /locus_tag="JN701_002313"
     CDS             1787..4573
                     /gene="polA"
                     /locus_tag="JN701_002313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY7364751.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4955..5587)
                     /locus_tag="JN701_002314"
     CDS             complement(4955..5587)
                     /locus_tag="JN701_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY7364752.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6169..6678
                     /gene="yihI"
                     /locus_tag="JN701_002315"
     CDS             6169..6678
                     /gene="yihI"
                     /locus_tag="JN701_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY7364753.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6867..8240
                     /gene="hemN"
                     /locus_tag="JN701_002316"
     CDS             6867..8240
                     /gene="hemN"
                     /locus_tag="JN701_002316"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY7364754.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8430..8540)
                     /gene="yshB"
                     /locus_tag="JN701_002317"
     CDS             complement(8430..8540)
                     /gene="yshB"
                     /locus_tag="JN701_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY7364755.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8651..10060)
                     /gene="glnG"
                     /locus_tag="JN701_002318"
     CDS             complement(8651..10060)
                     /gene="glnG"
                     /locus_tag="JN701_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY7364756.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10072..11121)
                     /gene="glnL"
                     /locus_tag="JN701_002319"
     CDS             complement(10072..11121)
                     /gene="glnL"
                     /locus_tag="JN701_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY7364757.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11295..12704)
                     /gene="glnA"
                     /locus_tag="JN701_002320"
     CDS             complement(11295..12704)
                     /gene="glnA"
                     /locus_tag="JN701_002320"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY7364758.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13077..14900
                     /gene="typA"
                     /locus_tag="JN701_002321"
     CDS             13077..14900
                     /gene="typA"
                     /locus_tag="JN701_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY7364759.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3864 a         3745 c         3852 g         3476 t
ORIGIN      
        1 caagaagtga atgctgcatt gttcaaagag tatttaccgt tattacaaca aagtgagccg
       61 accattaaac aaccggtaag atggaagaac gcactcggcg aactaaatgc caatctggat
      121 atcagtattg ccgacccagc caaatcttca tcatccacaa acaaagatat caaatcgctc
      181 aattttgatg tgaagttacc gcttaatgtc gtcacagaaa ccgcaaaaca gcttaattta
      241 tctgaaggaa tggatgcgga aaaagcgcaa aagcgggctg ataaacaaat cagcgggatg
      301 atgaccttag gtcagatgtt tcagttaatc acgattgaca acaataccgc ctcgctgcaa
      361 ctgcgttata caccgggtaa agttgttttt aacggacagg agatgagcga agaagaattt
      421 atgtctcgtg ccggacgttt tgttcattaa aatactcact cgcctctgct cattcagagg
      481 cgagcaaaat ttacttgtcc tgccgctgtg atgacatcaa cgacgtcaga agtcggtctt
      541 tttcctgcca cagtgagttc agccattgct gaaaatggcg cttaaagctt ttgtcattga
      601 tgtagtcgcc atgtaactca tcggcaatag gctgtaaatc cacatgaacg acaattcgcg
      661 tcagtttacc gcttaacata tcgaagaacg gctgacggtt attgtccgga taacacagcg
      721 taacattaag cagtttatcg aattgtttac ccagtacatt tagcgccatc gcaatgcctg
      781 ccgcctttgg tggcaacaag ttttgaaaag tggagtgggt ttgctgatgt ttttcttgcg
      841 tgaaacggga gccttcaacg aaattgacaa tggtggtggg atgcaggcga aacttttcac
      901 aagaacgccg agtggtttca acatctttac cacgtcgctc cggatgacgt aataaataag
      961 cgcgggaata acgcttcata aacggcatat ccagcgccca gcacgccagg ccaagaaatg
     1021 gcacccaggc cagctgttgc ttgaggaaat atttattcat cggaatgtgc ttacgaaaca
     1081 gcacgcacag tacgacaata tctgcccaac tacggtggtt acaaataagc agataccagt
     1141 tcttcttact taacccttcc agcccgtgaa cttcccattg caggtgtggg ttcagatgca
     1201 gtaataccgc cagaccttca caccagcaat acatcataaa atcacaaaaa cgcgaaacct
     1261 ttcgccaaat gactggcaca ggtagcaata gttttacaat cccggcaatg atgatcggga
     1321 cggaacaaaa aatagtgacc aaaatggtca atacgatact cagcaaaagg gttatcgcag
     1381 cgagtattct cgtcataatg aatttattca aaaggttagc catagacagt gcgctaaaga
     1441 agcgcaaggc gcagatttta gcagaaaacg acccaaataa cggatgatcc ttaaggagaa
     1501 aaataattca tatctatcca cattagaaaa aatcccatta tctcaattat tagggatggc
     1561 tttattttta actacatgaa aaacaaagac aaacatcata ctgtaaaaag catgataata
     1621 aattaaaagc gatgtaaata atttatgcac aaagttatcc acatgacgat ttgcgagcga
     1681 tccagaagat ctacaaaaga ttttcacgaa aagcggtgaa aaactcatgt ttttatcctg
     1741 tctgtggcat cctttaccca taatctgata aacaggcacg gacattatgg ttcagatccc
     1801 ccaaaatcca cttatccttg tagatggttc atcttatctt tatcgcgcat atcacgcgtt
     1861 tcccccgctg actaacagcg caggcgagcc gaccggtgcg atgtatggtg tcctcaacat
     1921 gctgcgcagt ctgatcatgc aatataaacc gacgcatgca gcggtggtct ttgacgccaa
     1981 gggaaaaacc tttcgtgatg aactgtttga acattacaaa tcacatcgcc cgccaatgcc
     2041 ggacgatctg cgagcacaaa tcgaacctct gcacgcgatg gttaaagcga tgggactgcc
     2101 gctgctggcg gtttctggcg tagaagcgga cgacgttatc ggtactctgg cgcgcgaagc
     2161 cgaaaaagcc gggcgtccgg tgctgatcag cactggcgat aaagatatgg cgcagctggt
     2221 gactccaaat attacgctta tcaataccat gacgaatacc atcctcggac cggaagaggt
     2281 ggtgaataag tacggcgtgc cgccagaact gatcatcgat ttcctggcgc tgatgggtga
     2341 ctcctccgat aacatccctg gcgtaccggg cgtcggtgaa aaaaccgcgc aggcattgct
     2401 gcaaggtctg ggcgggctgg atacgctgta tgccgagcca gaaaaaattg ctgggttgag
     2461 cttccgtggc gcgaaaacaa tggcagcgaa gctcgagcaa aacaaagaag ttgcttatct
     2521 ctcataccag ctggcgacga ttaaaaccga cgttgaactg gagctgacct gtgaacaact
     2581 ggaagtgcag caaccggcag cggaagagtt gttggggctg ttcaaaaagt atgagttcaa
     2641 acgctggact gctgatgtcg aagcgggcaa atggttacag gccaaagggg caaaaccagc
     2701 cgcgaagccg caggaaacca gtgttgcaga cgaagcgcca gaagtgacgg caacggtgat
     2761 ttcttatgac aactacgtca ccatccttga tgaagaaaca ctgaaagcgt ggattgcgaa
     2821 gctggaaaaa gcgccggtat ttgcatttga taccgaaacc gacagccttg ataacatctc
     2881 tgctaacctg gtcgggcttt cttttgctat cgagccaggc gtagcggcat atattccggt
     2941 tgctcatgat tatcttgatg cgcccgatca aatctctcgc gagcgtgcac tcgagttgct
     3001 aaaaccgctg ctggaagatg aaaaggcgct gaaggtcggg caaaacctga aatacgatcg
     3061 cggtattctg gcgaattacg gcattgagct gcgtgggatt gcgtttgata ccatgctgga
     3121 gtcctacatt ctcaatagcg ttgccgggcg tcacgatatg gacagcctcg cggaacgttg
     3181 gttgaagcac aaaaccatca cttttgaaga gattgcgggt aaaggcaaaa atccactgac
     3241 ctttaaccag attgccctcg aagaggccgg acgttacgcc gccgaagatg ctgatgtcac
     3301 cttgcagttg catctgaaaa tgtggccgga cctgcaaaaa cacaaagggc cgttgaacgt
     3361 cttcgagaat atcgaaatgc cgctggtgcc ggtgctttca cgcattgaac gtaacggtgt
     3421 gaagatcgat ccgaaagtgc tgcacaatca ttctgaagag ctcaccttgc gtctggctga
     3481 gctggaaaag aaagcgcatg aaattgcagg tgaagaattt aacctttctt ccaccaagca
     3541 gttgcaaacc attctgtttg aaaaacaggg tattaaaccg ctgaagaaaa cgccgggtgg
     3601 cgcgccgtca acgtcggaag aggtactgga agaactggcg ctggactatc cgttgccaaa
     3661 agtgattctg gagtatcgtg gtctggcgaa gctgaaatcg acctacaccg acaagctgcc
     3721 gctgatgatc aacccgaaaa ccgggcgtgt gcatacctct tatcaccagg cagtaactgc
     3781 aacgggacgt ttatcgtcaa ccgatcctaa cctgcaaaac attccggtgc gtaacgaaga
     3841 aggtcgtcgt atccgccagg cgtttattgc gccagaggat tatgtgattg tctcagcgga
     3901 ctactcgcag attgaactgc gcattatggc gcatctttcg cgtgacaaag gcttgctgac
     3961 cgcattcgcg gaaggaaaag atatccaccg ggcaacggcg gcagaagtgt ttggtttgcc
     4021 actggaaaca gtcaccagcg agcaacgccg tagcgcgaaa gcgatcaact ttggtctgat
     4081 ttatggcatg agtgctttcg gtctggcgcg gcaattgaac attccacgta aagaagcgca
     4141 gaagtacatg gacctttact tcgaacgcta ccctggcgtg ctggagtata tggaacgcac
     4201 ccgtgctcag gcgaaagagc agggctacgt tgaaacgctg gacggacgcc gtctgtatct
     4261 gccggatatc aaatccagca atggtgctcg tcgtgcagcg gctgaacgtg cagccattaa
     4321 cgcgccaatg cagggaaccg ccgccgacat tatcaaacgg gcgatgattg ccgttgatgc
     4381 gtggttacag gctgagcaac cgcgtgtacg tatgatcatg caggtacacg atgaactggt
     4441 atttgaagtt cataaagatg atgttgatgc cgtcgcgaag cagattcatc aactgatgga
     4501 aaactgtacc cgtctggatg tgccgttgct ggtggaagtg gggagtggcg aaaactggga
     4561 tcaggcgcac taagattcgc ctgaacatgc ccttttttcg taagtaagca acataagctg
     4621 tcacgttttg tgatggctat tagaaattcc tatgcaacaa ctgaaaaaaa attacaaaaa
     4681 gtgctttctg aactgaacaa aaaagagtaa agttagtcgc gtagggtaca gaggtaagat
     4741 gttctatctt tcagaccttt tacttcacgt aatcggattt ggctgaatat tttagccgcc
     4801 ccagtcagta atgactgggg cgttttttat tgggcgaaag aaaagatccg taatgcctga
     4861 tgcgctatgt ttatcaggcc aacggtagaa ttgtaatcta ttgaatttac gggccggata
     4921 cgccacatcc ggcacaagca ttaaggcaag aaaattattc gccgtcctgc gtttcttcta
     4981 caggctgcat ctcgctaaac caggtatcca gtttctgccg cagcttgtcc acgccttgtt
     5041 tcttcaacga agaaaacgtt tcaacctgca catcaccgtt aaacgccagt acagcttcac
     5101 gcaccatatt caattgcgct ttacgtgcgc cgcttgccag tttgtccgct ttggtcagca
     5161 gcaccagaac ggcgatattg ctgtctaccg cccactcaat catctgctga tccaaatctt
     5221 tcagcggatg gcgaatatcc attagcacca ccagaccttg caggctatga cgtttttcga
     5281 ggtattcgcc gagcgcacgc tgccatttgc gcttcatctc ttccgggact tccgcataac
     5341 cgtacccagg caagtcaacc agacgcttgc cgtcagccac ttcaaacagg ttgataagct
     5401 gggtgcgccc tggggttttt gaggtacgag ccaggctttt ctggttagtc agcgtgttca
     5461 gcgcgctgga tttacctgcg ttggaacggc ctgcaaaagc cacttcaatt ccggtatcgg
     5521 aaggtaggtg gcgaatatca ggcgcactca tcacaaaatg cgtctgttga taattcaaat
     5581 tagtcaaagc ggtcgtctcc gtcagtcaaa gctttgtggc gattatacct gaaccataat
     5641 aaaaggcgga tttttcggcg tgagcgttgt aagtaaaagc catacgcttt gtgagacatt
     5701 gccgatagtc tttatgcgaa atagcagaga aaattctgcg atgcatgtca aataagctat
     5761 ataaatcagt gaattgcttt tatataaaac tctgaaaatg tgcaaatact gatggcggtt
     5821 gattgtttgt ttaaagcgaa ggcgtaaagt agcacccata gagcgaggac gctaacagga
     5881 acaatgactc aggatgaggg tcaggagcgc caggaggcga agacagagga ttgtcaggaa
     5941 gacaaacgtc cggagacgta attaaacgga aatggaatca acacggattg ttccggctaa
     6001 aggaaaaaca gggtgtgttg gcggcctgca aggattgtaa gacccgttaa gggttatgag
     6061 tcaggaaaaa aggcgacaga gtaatctgtc gccttttttc tttgcttgct ttctgttaga
     6121 ttccgccgca aatcgatact gaataaaacg gctaaaagac gaaccattat gaaaccatca
     6181 tcttcaaact cacgcagcaa aggtcatgca aaagcgcgtc gaaaaacacg cgaggagctg
     6241 gatcaggaag ctcgtgatcg caagcgtcag aaaaaacgtc gtggtcatgc gccgggcagc
     6301 cgcgcagcgg gcggaaacac cacgtcaggc agcaaaggcc agaacgcacc aaaagatcca
     6361 cgtattggca gtaaaacccc tattccattg ggcgtgactg aaaaagtcac caaacagcac
     6421 aaaccgaaga gtgagaaacc tatgctttca ccgcaggcgg agttggaatt actggaaacg
     6481 gatgagcgtc tggatgcgct gctggaacgt ctggaagcag gcgaaaccct gagtgccgaa
     6541 gagcaatcct gggtggatgc taaactggat cgtattgatg agttgatgca gaaactcggc
     6601 ctctcttatg acgatgacga agaagaggaa gaagacgaga agcaagaaga catgatgcgt
     6661 ctgctgcggg gcaactaacg gattgccgcc gtgggccttc ccgttctgct gataaccctt
     6721 ccggttatat gttatctggt gtggttattc gttaaactac agcggttgtc gcggcgacaa
     6781 aagtggctgc gcaaccggct gatgacccga aacgggcatc cgccggtacg ccgtagccgc
     6841 cagagacgcc atcggaagga gtgagcatgt ctgtacagca aatcgactgg gatctggccc
     6901 tgatccagaa atataactat tccgggccac gatacacctc gtacccgacc gcgctggagt
     6961 tttcagaaga cttcggcgaa caggcgtttt tacaagccgt ggcgcgctat cctgagcgtc
     7021 cattatctct ctacgtacat atcccgttct gccataagct ttgttacttc tgcggttgca
     7081 ataagattgt tactcgccag cagcacaagg ccgatcagta tctggacgcg ctggagcaag
     7141 aaatcgtcca tcgtgcaccg ctgttcgccg ggcgtcacgt cagccaattg cactggggcg
     7201 gcggaacgcc gacgtatctg aataaagcgc aaatcagccg cctgatgaag ctgctgcgcg
     7261 aaaacttcca gttcaatgcc gatgcggaga tttcgatcga agtcgatccg cgggaaatcg
     7321 aactggatgt actcgatcat ttacgcgccg aaggctttaa tcgcctgagc atgggcgtgc
     7381 aggacttcaa caaagaagtg caacgtctgg ttaaccgcga gcaggatgaa gagttcatct
     7441 ttgcactgct taaccatgcg cgtgagattg gttttacctc caccaacatc gacctgattt
     7501 acggcctgcc gaaacagacg ccggagagtt tcgcctttac cctgaaacgt gtggcggagc
     7561 tgaaccccga tcgtctgagt gtctttaact acgcgcatct gccgaccatt tttgctgctc
     7621 agcgcaaaat caaagatgct gacctgccga gtccgcagca aaaactcgat atcctgcagg
     7681 aaaccatcgc cttcctgacg caatcgggct atcagtttat cggtatggat cactttgccc
     7741 gtccggatga cgagctggcg gtggcccagc gtgaaggcgt gctgcatcgt aacttccagg
     7801 gctacaccac tcagggcgat accgatctgc tggggatggg cgtttccgcc atcagcatga
     7861 ttggcgactg ctacgcgcag aaccagaaag agttgaagca gtactatcag caagtggatg
     7921 aacaaggcaa tgcgctgtgg cgtggtattg cgctaacgcg tgatgactgt attcgccgcg
     7981 atgtgattaa gtcgctcatc tgcaacttcc gtctggatta cgcccctatt gagaaacagt
     8041 gggatttgca cttcgctgat tactttgcgg aagatctcaa gctgctcgcc ccgttagcaa
     8101 aagatgggct ggtggatgtg gatgagaagg gaatacaggt gacggcgaaa ggtcgcttgc
     8161 tgatccgcaa catttgcatg tgctttgata cctatctgcg ccagaaagcg cggatgcagc
     8221 agttctcacg ggtgatttga atagtgaatg gcgcttcgtt tacaaagtag aaaacgaaag
     8281 cgccatcaat gtgctgagaa caagattgcc tgatgcgcta cgcttatcag gcctacaaaa
     8341 cctgttgaaa atattgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     8401 caaagagcac ttcatcagca atctgaaact caactaaaca gcccaatcat tgcggcacac
     8461 agcacggcag caacggcagt ttgcggtgtg tggctgtcaa ctgcgccact cgataccaga
     8521 ttaattattg attccagcat aatgactctc cccgttttcc gggcagatca tactgaactt
     8581 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8641 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     8701 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgcaac gcggtcgtca
     8761 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     8821 gcgctctgtc tgcccactgc gcaagaagcg tcgcccagct gtccggttgc atttgcgaag
     8881 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     8941 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9001 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9061 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9121 gttcgcgcag cggcggtaga tgaacgcgga taacgtttag gcggtggaac agatcctcac
     9181 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatccgca
     9241 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9301 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9361 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9421 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9481 cgataaacgg tgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9541 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9601 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9661 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
     9721 gcgccacggc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
     9781 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
     9841 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
     9901 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
     9961 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10021 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10081 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10141 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10201 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10261 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10321 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10381 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10441 gctgggatcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10501 taccacgcgt tcagccactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10561 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10621 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10681 accgccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    10741 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    10801 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    10861 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    10921 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    10981 caatttgcgg gagctttggg cgagcagttg ttgcgcggca gggttggcgt aatggatctc
    11041 caggttgtca tcgattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11101 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11161 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11221 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11281 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11341 cgcacgcggt catcttcttc gcgacgcaga gcgatgtacg catcaattgc ttcgtcagtg
    11401 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11461 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11521 ttgtccatgg cttcacccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11581 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11641 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    11701 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    11761 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    11821 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    11881 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    11941 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12001 tcgtcagctt ttttggtcat ggtattgaag cgggtagcca cttcgttctg accagcagtc
    12061 gctacttcgt ggtgatgggc ttcaaccacc agccccatct gttccatcac cagacacatt
    12121 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12181 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12241 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12301 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12361 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12421 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12481 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12541 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12601 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12661 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    12721 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    12781 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    12841 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    12901 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    12961 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13021 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13081 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13141 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13201 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13261 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13321 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13381 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13441 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13501 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13561 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13621 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13681 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    13741 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    13801 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    13861 gtatcgaaac cgacctggcg gaagctggcg atatcgttgc gatcacgggc cttggcgaac
    13921 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    13981 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14041 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14101 acgttgcgct gcgcgtagaa gaaaccgaag acgctgatgc gttccgcgtt tctggtcgtg
    14161 gcgaactaca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14221 tatcccgtcc gaaagttatc ttccgtgaaa tcgacggtcg taaacaagag ccatatgaaa
    14281 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14341 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14401 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14461 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14521 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcgctgt
    14581 tcggtctgca ggatcgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14641 agattatcgg tattcatagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    14701 aactgaccaa catgcgtgct tccggtactg acgaagccgt tgttctggtt ccgcctatcc
    14761 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    14821 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    14881 gcgcaccgaa agactattaa tttcgttttt tagttataaa aaaacctgcc atcgctg
//
LOCUS       DADLTD010000096        14643 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLTD010000096 DADLTD010000000
VERSION     DADLTD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.096"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN701_002322"
     CDS             complement(<1..209)
                     /locus_tag="JN701_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7364760.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN701_002323"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN701_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY7364761.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN701_002324"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN701_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY7364762.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN701_002325"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN701_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY7364763.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN701_002326"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN701_002326"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY7364764.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN701_002327"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN701_002327"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY7364765.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN701_002328"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN701_002328"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY7364766.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JN701_002329"
     CDS             complement(4598..5320)
                     /locus_tag="JN701_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7364767.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN701_002330"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN701_002330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7364768.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JN701_002331"
     CDS             6520..6738
                     /locus_tag="JN701_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY7364769.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN701_002332"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN701_002332"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7364770.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JN701_002333"
     CDS             complement(7472..7672)
                     /locus_tag="JN701_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY7364771.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN701_002334"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN701_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY7364772.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN701_002335"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN701_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY7364773.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="JN701_002336"
     CDS             10169..12082
                     /locus_tag="JN701_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7364774.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="JN701_002337"
     CDS             12082..13104
                     /locus_tag="JN701_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7364775.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="JN701_002338"
     CDS             13098..13316
                     /locus_tag="JN701_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY7364776.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="JN701_002339"
     CDS             13370..14239
                     /locus_tag="JN701_002339"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY7364777.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14557)
                     /locus_tag="JN701_002340"
                     /pseudo
     CDS             complement(14294..14557)
                     /locus_tag="JN701_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
BASE COUNT         3614 a         3997 c         3604 g         3428 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggttcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttatcgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcaaggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcaa caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caagcccaga gccgcattaa gatgcttgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggctcg gcataaaccg
    12301 cgtttagtga tatttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgcc ccgttacttt attcgtaccc
    14581 cttataatgg ggtgttagcc agccagaccc ggcatgatta ctgcccccag tcgtccatga
    14641 tcc
//
LOCUS       DADLTD010000097        14516 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLTD010000097 DADLTD010000000
VERSION     DADLTD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.097"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1264)
                     /locus_tag="JN701_002341"
     CDS             complement(<1..1264)
                     /locus_tag="JN701_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7364778.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAA"
     gene            complement(1267..1797)
                     /locus_tag="JN701_002342"
     CDS             complement(1267..1797)
                     /locus_tag="JN701_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAY7364779.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(1790..2686)
                     /locus_tag="JN701_002343"
     CDS             complement(1790..2686)
                     /locus_tag="JN701_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAY7364780.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(2690..3040)
                     /locus_tag="JN701_002344"
     CDS             complement(2690..3040)
                     /locus_tag="JN701_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAY7364781.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(3037..3618)
                     /locus_tag="JN701_002345"
                     /pseudo
     CDS             complement(3037..3618)
                     /locus_tag="JN701_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
     gene            complement(3615..4250)
                     /locus_tag="JN701_002346"
                     /pseudo
     CDS             complement(3615..4250)
                     /locus_tag="JN701_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
     gene            complement(4243..4710)
                     /locus_tag="JN701_002347"
     CDS             complement(4243..4710)
                     /locus_tag="JN701_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7364782.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(4848..5255)
                     /locus_tag="JN701_002348"
     CDS             complement(4848..5255)
                     /locus_tag="JN701_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HAY7364783.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(5252..5644)
                     /locus_tag="JN701_002349"
     CDS             complement(5252..5644)
                     /locus_tag="JN701_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAY7364784.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(5641..5964)
                     /locus_tag="JN701_002350"
     CDS             complement(5641..5964)
                     /locus_tag="JN701_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7364785.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(5967..6167)
                     /locus_tag="JN701_002351"
     CDS             complement(5967..6167)
                     /locus_tag="JN701_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7364786.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(6167..6661)
                     /locus_tag="JN701_002352"
     CDS             complement(6167..6661)
                     /locus_tag="JN701_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HAY7364787.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(6829..7575)
                     /locus_tag="JN701_002353"
     CDS             complement(6829..7575)
                     /locus_tag="JN701_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY7364788.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(7621..8493)
                     /locus_tag="JN701_002354"
     CDS             complement(7621..8493)
                     /locus_tag="JN701_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HAY7364789.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(8490..8672)
                     /locus_tag="JN701_002355"
     CDS             complement(8490..8672)
                     /locus_tag="JN701_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAY7364790.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(8696..9532)
                     /locus_tag="JN701_002356"
     CDS             complement(8696..9532)
                     /locus_tag="JN701_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HAY7364791.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            9687..11426
                     /locus_tag="JN701_002357"
     CDS             9687..11426
                     /locus_tag="JN701_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7364792.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDQKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVVNHGW
                     KHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKPFAPR
                     PMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQARVI
                     EEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQIMRA
                     GRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVINEPIG
                     GEGENERFTVMEF"
     gene            11426..12472
                     /locus_tag="JN701_002358"
                     /pseudo
     CDS             11426..12472
                     /locus_tag="JN701_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(12946..13536)
                     /locus_tag="JN701_002359"
     CDS             complement(12946..13536)
                     /locus_tag="JN701_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7364793.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(13600..13911)
                     /locus_tag="JN701_002360"
     CDS             complement(13600..13911)
                     /locus_tag="JN701_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein"
                     /protein_id="HAY7364794.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(13916..>14516)
                     /locus_tag="JN701_002361"
     CDS             complement(13916..>14516)
                     /locus_tag="JN701_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stability/partitioning protein"
                     /protein_id="HAY7364795.1"
                     /translation="KKANVMRPVEYQNGEAFTIRNVQVMPESIPAGFKALADMSPFES
                     LLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSHHIAN
                     TIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVMVVGG
                     GAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
BASE COUNT         2945 a         4041 c         4004 g         3526 t
ORIGIN      
        1 cggcagcaac ggtcgatttc gcaagaatat cccgcccgac ttttgtcagg gttgccaggc
       61 tggcgacatc attccccgta aaatacggaa acctgtctgc cgcagtagca agcccggcca
      121 gcgccgtcag ggtggcatct ttcggttgct tacccgcaag cgcgttagtc atggtggtcg
      181 caaaattcgg ggcgtttccc agcgccgccg ctaactcgtt cagcgtattc agtgcgtcag
      241 gtgacgaatc tacaagggcg gcaatcgcag ccataacgaa agccgtgctt gcgatttggg
      301 tattattcgt tccctgtcgc gcagctggcg ttgttggcgt tccggtcagc gcaggactgt
      361 ttaagggcgc tttcttgttc gtttcatcca ttaccgcctt aaccgctttc gatgtcgcgg
      421 ccagtgtttc agacgtgctg ttggtggcac tactgagctg gactatccct ttttgtgccg
      481 tcgtggcgtc ctgagcggta tattttgcgt tagcaaggtc atacgcggcc tttactgctt
      541 tcggcgttgc agcctgcgtt tcagacgtgc tgttggtggc gctactgagc tggacaaggc
      601 cttttcgcgt cgtgctggca tcctgcgcag tatatttacc gttagcaaga tcatacgcgg
      661 cctttactgc tttcggcgtt gcagcctgcg tttcagacac gctgttagtg gcgctactga
      721 gttgaacaaa gccttttgcg gtcagcgagg cgtccgggtg acgtcgtgac tgttcatgct
      781 ctttcagttt gtcatccacg taatccactg tggccatcac catggtgttg tccacggtaa
      841 gcgccacggt ggcagtgctg gatacggtca gaatggtgcg aaatgtttgt gcacgaccgg
      901 acccttcggc aacggctggc ttgtaacttt cggcagtatt tcccaccgcg attaaatcgc
      961 cgtgctcatc aaatacacca atttcccgga tccagaatcc gcccgtttca ggaggaataa
     1021 ccagctccgc aataatgcgg ttctgatgtg ttgcgtccag gatgacgcga ttaacagtat
     1081 gtcgccacac ctcatgcacc agacgggtct gcttactgtc tggtgtgggc aacgtgccgc
     1141 caccgtcgcc cacggccata tgagtcaggc ggacaggctt accatctggc gcggctacct
     1201 gagctaattt ttttgcaccc gtatcggtga taatggtttt aaattttcgt gttgtggtac
     1261 tcatgcttaa tcgcctggat aaatggtaat aacttcaccg tcataagttg ccgccgctgc
     1321 gaaaatatcc cccggaattt cctgaatgat attcagccct gtcatgtggc ggctgaccgg
     1381 gcgggcatca gcaatcaacc gctccatttc cagatacatt tcctccgtca cgccactgtc
     1441 cagcgtgccg acttcaacgg taaatgttcc cggttctccg ccgaactccc accactcaga
     1501 cacgcgaatg aggtatccca gcggctcaat ggcccggcgc agtgcgctga tggtcccttt
     1561 gtgtcggtgt atcagccatg catcacgaat aacctgtcgc tttgtctctt ccggccagtt
     1621 gcgatcccag cggtcaacgg aaaacgccca ggcgagataa ggcagcagat gcaccgggca
     1681 ggtgtccggc gaccacagcg tgttgaggtc taccggaatg tctgtaatgc gtgttccgac
     1741 ggcttcggca caacgcatga aattgctggc tgatggtggt aacaacgaat tactcattgc
     1801 gtccaccttc gctgatggtg aatgactcac agcgcgccgc ctgtatgtcg ctgatggcca
     1861 tattctgtgt gggttcgatt atctccacgc gttgcacacc gtgcacatgc agtgcggcag
     1921 caatggcgga caacgccacg tcctgaccga taagcccctg ctcagccagc cacttcctga
     1981 acgacgattc agccgcggcc agaataggtt cggattccgg gccggggtaa aagtacagtt
     2041 ttgcattcag ccgccatgtc acgattctgg cactctgtac ggtcaggcgg tcggccaccg
     2101 ggcgggtatc ctctgcattc agaacggcgc gaacggtatt aagcaacgcc tccgttgctg
     2161 tgccgtcgcc ctcagtggac aggatggaaa ccgtcacgtt ggccggagac gggctgatgg
     2221 cccgcgcatc gcgtaccaga ccgctggcgc tgcgtgcaaa atactcgtat gcacctgacg
     2281 ggccagcaac actcagaccg tcatatgccc gttgcgcccg tagtctcagc gaggtgtcac
     2341 tttccatcac tgcatcggtg gtatccgttg ccggagtgat aaccaggcgc tttgtgttca
     2401 tattgcccgc gaggttgtcc aggtctgtcc cggcgctgtg gcttagcatg caggcgcgtg
     2461 caccctcatt gacccgctgg cgtaacagca tttcacgaaa cgacatggtt tgagcgataa
     2521 cgttaagggg ttccgattcc agctccagcg cggcggaaac ggcttcacgc tgttcggcgg
     2581 gataggacgc aatcatcatg gcctttgtgt cagccagaat tgcctcaaag tcaggctccg
     2641 cgatgatggc gggttccggt aactgtgaaa ggtcaacggc aggcatgatt tactccctca
     2701 gcgtgatggt taattcaaca ttctgcatgg tctgcatgac agtgcccgac agcgtcaccc
     2761 cggcgcggcc tcctgccttc cagacaacgt cgatagcgtc cagggcaatg cggggttccc
     2821 atcgtgtcag cgcaatcacg gcagcactca tgcattgcag acgcgtggtg ttattcatgg
     2881 gttcgtcaat caaatcaggc acaaggctgc catattcccg tcgcataacc cggcttgcca
     2941 gcggggtggt caggatgtcc ctgactgact gtttcaggtg ctccatatcg ttcaggtttc
     3001 ccgttccgtc cgggttcatt cctgtgtagc gggttgtcac tgcgggcctc ctgtcgaatc
     3061 gctgccacct ttaacgccac cgtgtttatg cgtatgcact gtgatgccgt ttgaggtgaa
     3121 attgccgccg ctgtgcgtga tattgccgct catctttccc ccttttgtga cgtcaagcgt
     3181 cgccgttctc agaaggtctg tgcattccac gacgggcgta tccagtgtca cgctgacgga
     3241 tgcctgcagg gtggctgttt tcatgccgct ggcgctcagt gcgcctgcgt ccgcgtcgta
     3301 gcggaacacc gcgccatccg gcgcgctgac cacgatttct ttcaggcttt tgccgggggc
     3361 cggaatggca tcactccaca ggctgccaat tatcatggcg gtttccgggt tgccgccaat
     3421 gcaggcaatt accacctgtt cgcctggtga tggcggcagc cacacattga aggctcccgc
     3481 gcgagaggtg ttccagcgca gccaacctgt ttccagttcg ccgctgcgaa cgcgcacgcg
     3541 ctaggacttc tcatcaactt cagagatgat cccggtgcgg atgatattgc tcagtagccg
     3601 catgagttct gcgctcaccg tacagcctcc gcaatccggc ccagcaccgt gttataaatc
     3661 aggcgctcat ctgcctggct gatacccagc agctaacgta ccgggtaatc ggtgaaaatg
     3721 cccggcgcaa cctgatcgcg ctcaccgaac tgatgaacgc gtgcaatacg tgcggccacg
     3781 ccgctgtaac ccaccgtcac accggaagca tctgcacggg ctttcaggta gcgggcggtg
     3841 cgcagtttta cgaacatggg gacgcgcttt gtgctgtcct ggttgatgcg ccgggtgcgt
     3901 atttccagaa agcggtcgat gtcatcccgg taaaacgtgc ggatgctgtt tttatcctca
     3961 tcccacccgg tgatggttcg cccgtatttc cccgtgtcgt gatgccagtt tttcagcgtg
     4021 cgtgcttcgt tattccagat aaagcgaatg cgctcctgta tccgggttac gtggcgtctg
     4081 cgtggtgtcc atgcggtccc gtccggcgct ttctgtggcc ggatacgtgc ctgctgggcg
     4141 cggcgtaaat cctgtgccag ctttctggcg atgttattga tggcctgctg attcaggctg
     4201 tcgcggatgg cctcaaaggt ttcatccacg cgagtgaatg ccttatccat cgctttcacc
     4261 ccacgtcaca tcctggaata catgcgacca gtcgccttcg gaagatggca ggcggggttt
     4321 tggctccagc aggtgttctg cctgcggtgt gccctgactg ctgcgcgtga tgcgaacgcg
     4381 ttcccgcagg gggagcgtaa acaggagatc ggcgctgtca tcgtcattga taacggcgga
     4441 aaatttgatg tcctgattac gctcagggtt gagcaacaac tgtggctgat tttcggataa
     4501 ccacgccagc agcggcagcg tgaggtcgtc cagctccccg gcgtaatcca tgacaaacat
     4561 caccatctga tagcggtaaa caaacgatgg ggtttctccg gtcgtttcaa tgttgccgct
     4621 ctccacgaaa atggtgaatt tttccgggtt agcctgacac catcggcatg aacgggtcat
     4681 ggcttcacgc agggaatcag ttttcagcat ggttgttatc ctcgttgttc agtcgttgca
     4741 gcctgcgctg ttccagtaat tcaatggccc gtttatccgc gttacaggtt tccagtgcat
     4801 ccagaaggcg gtcgccccat ataccgagat ttccccatgt gggagtgtca gggaaggggg
     4861 gaggcgttac cggtatggtc agcgtctgcg gtataagccg gactgacggc gctggcagtg
     4921 gcgcgttctg cgtgcctgcg cagcctgtca gtaaaacgag cgtcaggcaa agcgtgggcg
     4981 cattcatctt ttgcaatatc gttgcgtagc tgttcacgtc ttacctctcc gtcctgattg
     5041 cgttgctgat tttccacgcg gagttgcgcc agcacctgct gcatatcctg taccccggcg
     5101 ctgatgatat tcagggtgtc ggcggtactt ttcagggtgc tggcctgcgc ttcgtttctg
     5161 gcgttctccc ggcccagcga ccacgacaga cgcatggatg ttccccatcc ggcagtcaga
     5221 aggaaagcga cgccaagcgt gggccagagc ttcatgccgg ataggctccg tgtggtaact
     5281 gaaaatgcgg tccgtctttc agggtcttcc agtcgccgcc ccattccacc ggaatattca
     5341 gttcccggct ggcctgtctg aatgctgctg cgattttttc gtacagcggc cattcccatg
     5401 acatctggct gccgatataa gccacaacat ccacggcatg ccccgtaagg tggcggctgt
     5461 tcatggtctg gctcttaccc gtggccacca gttgcttctg gcggtaacgg ctgcgcaacc
     5521 cttcggtgat accaaaatcc acttccgaga tttccagtgc ccgtcgggtc actttcacca
     5581 gatcaggatt tacaccctgc aaattctttt cgctccggct gctgaattta aatgtgttgc
     5641 tcattcgtcc ttctccttca ccctgcgatt aaaggccgca ataaccttgt cgcgtgcttt
     5701 ctctgcaccc ataaaaccga ttgatgcgcc gataaacgtc acggcatctt caggaaaccc
     5761 gaagaagcgc aacgacccgg ccacggccat ggcaagaacg ccgcacgcca gcgatcccgt
     5821 tacggtctga accagtgttc gtccgtcata aaggctcatc agcgcggaaa tgctgaccgc
     5881 cgcgcctact gcatacaccg ttggcaggtg gtcaaagagc cacgcaataa cctgctctgt
     5941 gatccctgtt tgaatggtgc tcactgctac tccccccaca actgaatcat ttctcgtttc
     6001 ttcttctccg gctccggcat ctccacttcc tgcccggcgt ccagaaatac ctgctgacag
     6061 agtccggggt tggcatccag caccttttcg gtgacgccct gcgtcgtgcc gtagtaccgg
     6121 aaacagagcg aatccacggt gtcgccttcc agtgccttca ctttcatcag cacaactccg
     6181 caaagattcg cgggcggcac agaatgtcag agatggccca gctcacatca cgccacaaat
     6241 ccgatgtctg tatatccaga gcgtctgccc ggcggtcgcc cttgtccgta gtgtctgcat
     6301 cacgataacg ctccagaatc agggcgcgcg tggcggtata aacagcattg cgccagtgcc
     6361 agagattgac gctttctccg ttaattacgg gtgccggaac atcggccagc gtctgatggc
     6421 cagctgcctg ctgttcctgc tgccatgctt ccagctcgcg ggtaacgtgt gccacagccc
     6481 cggtggcagt atgcagcagg cgggaggtgg tcacgcgccc cggcagtcgt accgccagac
     6541 gcagctcgcg cagcacaata tccggccaga atgcacccgc tgaaatgcgg gtatcgccat
     6601 catcggtatc ggtgatgtcg tcctctgcgg gtccgggttc agttctggca actatagtca
     6661 tggggttcac tcctgaaaaa aaatcgggcg gtgggtgcgc ggtgtaaacg gtcacggagt
     6721 caaactggaa caccgcgcac gccgcccgct gacggggtca gtcgttaacc gcgcttcgcc
     6781 ttctgcgtcg cggtggtttt tcgtgttgca ggcttccgcg ttgtcttttt acttttgctg
     6841 ctttttgctg ctttcgtcct gcgcctgctg tgcgctggcg tcttctggtt cggctgcgga
     6901 atcggctttt ttcagggcgc gggaaagggt tgcaatctcg cgtttcacac ctgcgttcgg
     6961 gttcaggtgc attgcttcgc gcagcagctt cagtgacagg gccatgctgt ccgcatcact
     7021 caggccacgg cgggcaaagg cgcacgcttt gcataatttg gcgcgcactt cgtccggcat
     7081 gtcctggtcg gtgacaatct cccggagggt gtccagtggt tcgataaagg cggacaaatc
     7141 cgcgtcggca tccgtcccgg cctgcgtcag taccggatta cagatttctt cggtcagtac
     7201 cgtggcagca gtacggccaa agttatccgg catgatgagg ttgtgacgga ctacatacgc
     7261 gccgatacgc agcgcaagcg gaagatcgcc acagtcaatc gcccacacca tcagcgtggc
     7321 aatcacttca tcctgctgcc cgccgtcagc ctccagcgtt ccctcaatcc agccggaaaa
     7381 atccggcaac aactcttttt tgatggcggc tttcgcgctt ctggcctgta cgcccttaag
     7441 ccgggcctgt gccagacgca gacgatacag cacctcttca tgcgcggtac gcgcggcgtg
     7501 gtccacgcct tcattcgccc ggcctgcgcg ctgtgccatc acgttctgcc agtgttgttg
     7561 tgcaggagta atcatttttg ctctccgtta caggcgggca tgatgcccgc cgtgagttga
     7621 ttagctgtcg gcgaacttca ggccagtaac catcgcgcac ttgccatagt cttcaacgac
     7681 ataagcgtca ttgatggact ggtaggtggc gatgcggttg tattccggct cgtctttcat
     7741 caggcgacgc attgaacctt tctgccagta aatcgacagg ttgttgaacg aggtgatcag
     7801 catcgttgca tccgggaaga acggcgcaag gaacacgccc agcccgccaa tggtgcgcga
     7861 tgacaggatg agctgcccgg cgagtaattc cgcattggga ttctggccgc tgatgctgtt
     7921 cagcacgggc agacgcagcg agttaaacag gttgcgcccc ataatcacca cgaggtcgtc
     7981 agcttccttg tgccattcat ccagcaggga tgaacgcgcg tcctgtacca gtgcatcagc
     8041 gttcgcatac ttacccgcgt gcgccacggt gttgtccatg ttgcgggagg tcagcgtcac
     8101 gtcattcata acgcgttcgc tggcgtcggt tctgatgtgc tccagccagc ccacgttaac
     8161 gtcctgaagc agcttgttgg tgctgaagtt ggactcatcc gcgtgagacg tgccgttgaa
     8221 accgatcatg atgcggtcaa gcgccacctg tcgggcaatc tgtgcgctga tgcgggactg
     8281 aaaatcaggg tgtgccgacc aggcatcaag ctgcggatac gaaataaacg tgtcgtagtt
     8341 cacctgttca cactgatatt tgcggttttt cagatcaacc acgttattcg ggttacggcg
     8401 ttttgtgccg tcataactgg tattcgtgcg cgcaatcggt cctgtggtat ccaggaggat
     8461 tttttcgcct ttctggtcgg tcacgccgat cacgttattt ttttcgtaaa ttcggtactt
     8521 tcctttgagg cgttttcaaa acgctgctgc accgcgggtt ccacggtaaa tcgcgacacc
     8581 agtgcagata ccgggatatt attaagcgac acctgctgcg ccatatagca acccagcttg
     8641 ttgcgggtaa tatctgacat caccagattc ataaaaaatt tgctcctttg tctaatcaga
     8701 agtcggccag ctggtcggag gctgcgcccg ttgcggtgaa gcggttctgc ggatcgccgt
     8761 cctgcgtgcg cagtttttcc ttcagtgctg ccagctctgt ggtcagtgac gtaattttct
     8821 ggcggtcctg ctgatggcgg gtttccagca cattaaaacg gtcgataatg tcggcctgtg
     8881 acgttgcgac gccttccacc gcttcctgaa tacgggaaaa actggcgtca tccgctttgc
     8941 ggccacggcc aataatcccc attacgcggt taaaccactg ggtgccttct tcctggcgtt
     9001 gttctgccat ttcgatgatt tcagactcga tggcttcgga gataagcggc gcttcaccct
     9061 ggatactgtt aaacgtcatc accgcctgac gttgctgtgc cgtgaatttc aggcgctcag
     9121 tgcccaggct tgccggggtg tcggtcatcg ccagcccgac cagataggcg cgcccgttaa
     9181 cggagaactg cgggtgcagt tcgatactgg aatagatttt cttgccgtcc gcaacaagct
     9241 gcttcatgcg ctcggtcggt tcgatttctg catacagcgc agtacgtccg gccagcggac
     9301 cttccgtaat gtcttccgta ctcagtgcgg tgacatcgcc cattgcggaa aattcgcttg
     9361 acgggcatgg cgagagatag tgctcaacgt tcacgcgggc agcgtaaaca tccgggttga
     9421 agttctcggc ggcttcacgc agatgcaccg gactgatttc acggccatca acagttgatc
     9481 cggagacagc cacgcgaaac tttttgcggg atgtcttttt ttcattagcc atagtttttg
     9541 cccctctgac tggttcttca gtcatgatgg caaagcgtaa caggctgata caaagggctt
     9601 ttgttgtaag aaaacggcca gaacagaggg ttaaggagaa cagtttcgcg cgcgggtaat
     9661 cttcctgtaa ttactcaggg ggagcaatga ttcaggacgc ttttgtgcgc cagcgtgcgc
     9721 ggcaacttta ctggcagggt tatccgcccg cagaaatatc acgtctgatg ggaataaacc
     9781 cgaacacgat ttatgcgtgg aaaaaacgtg accagtggga tgaaacgcca cccgggcagc
     9841 gtgtcacgca gtccatcgat gcgcgcctca tccagcttac tgaaaaacag aataaaacag
     9901 gcggtgactt taaggaaata gacctgctga cccggcagct taaaaagctg catgatggcc
     9961 agccggatgt gatggccgca ggaaagaaag gccgggcgaa aaaactcaaa aatcatttca
    10021 cgccggaaca gattgccgca ctgcgggaaa aaatcatcag caggctggag tggcatcagc
    10081 ggggctggtt tgactccctg accctttgca gggaagccgg gatacgtaac aggatgatcc
    10141 tgaaatcccg acagattggg gcgacctggt attttgcaca ggaagcactg ctgatggcgc
    10201 tgcgtgacga tgtggcgcaa ccttaccagc gtaaccagat ttttttgtct gcgtcgcgtc
    10261 gtcaggcgtt ccagtttaaa agcattattc agaaggccgc ggctgaagtt gatgtggagc
    10321 tgaaaggggg cgataaaatc atcctctcca acggcgcaga gctgcatttt ctcggcactt
    10381 ctgctgcgtc ggcacagtcc tatacgggca atttttattt tgatgaattt ttctgggtca
    10441 gtcgctttgc tgaactgcgc aaggtggctg gcgctatggc aaccctcagc ggactgcggc
    10501 gcacctactt ctccacgcca tccaccgaaa cgcacgaggc atacgcctac tggaacggcg
    10561 accaaaaggc ctcgcataaa cgccagcgtt tttctgtgga ctggaaaacg ctgcataacg
    10621 ggcttatctg ccctgaccgg acgtggcggc aaattgtcac gctggaagat gtggttaatc
    10681 acggctggaa acacaccgat atcgacgaaa ttcgtgatga aaacaccgaa gacgagttcc
    10741 tcaatctcta tatgtgtgag tttgtccgcg aaggggaatc ggcatttaac ctgaatatcc
    10801 tgattggctg cggcgttgac ggatacgacg actggaaaga ctggaaacct tttgctcccc
    10861 gcccgatggg gaatcgtccg gtatggattg ggtatgacgc aaacggcagc agtggcaacg
    10921 gcgacagcgg cgctgtgtcc gtggtggttc ctccgtctgt tcctggtggc cgttttcgaa
    10981 cggtggagac gcgacgcgtt caggggctgg agtttgaaga acaggccaga gtcattgaag
    11041 agttcacgtg tcgctacaac gtggaacaca tcggcattga tgtgacgggc gggaacgggg
    11101 aggctgttta tcagatagtg aaacggtttt tccctgccgc tattccgtac accttcacgc
    11161 tgtcatcaaa acggtcgctg gtactgaaaa tgctgcaaat aatgcgtgcc gggcggtggg
    11221 aatacgatcg cgccgaacgc gagctggtcg cggcctttaa cgccgtgcgt aaggtgaaaa
    11281 caccgggcgg ctttatcact tacgaaacgg accgagcgag ggggatcagc cacggcgacc
    11341 ttgcgtgggc aaccatgctt gctgtcatta acgaaccgat tggcggcgaa ggagaaaacg
    11401 agcgtttcac ggttatggag ttctgatgag cagaaaaaat aaaaaagtgc gcatgagttc
    11461 acgcattgat ctcgctgatg cgctcaggaa agaatcatcg ctcagtgcat tcacatttga
    11521 tggtccttat cgcctgaccg ggcatgacct gctggacaat atgtactgtg ctgataacgg
    11581 gcggtggtat gaaaccccgg tggactggta cggtctggca agagctgccc ggcaaacgtc
    11641 ctagcatcag tctgcgcttt attttaagcg caatgtattg ctcggttgct acatcccgca
    11701 cccgctgctt tcccggcagg atttctcggc gctggcgctg gactggtttg tgttcggtaa
    11761 cgcattcctt gagcttcgaa gcaatatgct cggcgaaccg cttaaattac ggcacgccct
    11821 ggcgaaatac atgcgacgcg gaagcgatct tgaatcatgg aggtatgtgc aggatggcaa
    11881 ggatgcgttt cagtttcgcc caggcaaagt gtgtcacctg atgaatcctg acattaacca
    11941 ggaaatctac ggcatgccgg aatatctcgg cgcattactc tcggccagcc tgtctcattc
    12001 ggcggacatg ttcagaaaac tgtattacga caacggatcc cacgccgggt gcatcatcta
    12061 catcggtgcc gcgcaggtaa accgcgaaag catggactcc ctgaaagaaa cgctacaggg
    12121 tgcgcgtggt ggtggtgcgt ttaaaaacgt gctcattcat gcgcccaacg ggggcaaaga
    12181 gggggtgcaa attttgccgt tccagcagat caccgcaaag gatgagttca tgaatgttaa
    12241 ggcggcatcc cgtgatgatg tgctggctgc gcaccgcgtt ccgccgcaac tgatgggggc
    12301 gatgccgggt gaaaaaagtg cgtttggtga tgtggagaag gccgcgcggg tttacgcaat
    12361 taacgagctg atgcccgtca tggaggccat gaagcacatc aatgactggc ttggcgaaga
    12421 ggtgatccgc tttaaccctt acgcactgtt agacatccag cccacatcct gacgcgcttc
    12481 gcttgtctgc tgcttcgccg gggcataaaa aatttatgcc ccgactctcc agctcctgta
    12541 tcagtcagat aatttcacga cgccttccag tttatcgcca ccatcgacgg tcagactctt
    12601 acgcaatccc accgcgttga ctgcatgttc tcgccgcctc agtgcgattt tgacggcctt
    12661 atcagaatcc ctcacgtatt tttcacgctc agcgtgagaa atacggccat tctgtcgtgt
    12721 cgctgcgaca tcgttaaggg aacgctattt accccctgaa acgcgggctg ttcccccgtc
    12781 acctgcgcgc agaaaaagcg cgtttttttg tgcacgcacg gatccttgac ggatccagcc
    12841 gccacgcggg ctggaagggc aaaaattcgt tcaaaaaaat tgtgcaaatt tgtgcactat
    12901 tgtgcagcag ggcgatgtgc gattatcgcc ctggtttgag ggggatcagg tgttattttt
    12961 atctgtcgtc agtgggctaa accatccaca atttacatcg catgactgat gcgttctgtc
    13021 ctcgtgtttc tcctgccaca atgcttttcc gccagccttt atgatggctc tttcaacatc
    13081 ccgctcgtat cgcaagggct gaaatacgtc attaatgaaa tattcgctta tgtccggaag
    13141 cgatactgca aaattatttg tttgttgcgc cgtcctgcta tgcagcaaag cggcatgctt
    13201 taatgcgtcc tgtgctttct ggaatttaag tgcatagtca gtggcgattt tttccagttc
    13261 agcgattcgg ttgtctttgg tttccagcca atcaagtagc gccaatactt caggatcccc
    13321 gacatccaac actgttacgc gtgatttttc ataatgttcg tcagcaagag ttcggccaat
    13381 tttgaaatct ccatcatcac cataaccgga gcaggcataa acaacctgtg ctccagatat
    13441 tcgctgaatt gacatttcct cgccgcaaat agggcatttc ggtactggca caggtgaata
    13501 acgttcacgt aatgcctgat agttgatttc gctcattaag tcgttctcct tgtttatgtt
    13561 ctgaggtgga gagcagggcg aactcgccct gatgttgtgt tattcaggaa ataacgcccg
    13621 gatatttccg gccatctgac tggttatctg tgcggttgat actggctgtg atgcggggcg
    13681 ttctgtcctg gtttgtgtca ctgataacgc ttcatcacca gcccatgcag ccagtcgata
    13741 agcctctgcc ggattcattt tcagaagtgc cagcccggcc agaaaagcca cgcgttggcc
    13801 gcttttgcgg gcttctggtg taaggctgtc cagccaggcg catgcttctc cttcgttctt
    13861 gacggcggtg ggcttcagat agaaacttat ccgtctggtt ggagtcgtca ttggtttact
    13921 ccttgtccat tgcgtacagc ccattaacca gagcaaactg tggcaccccg tccgcgatga
    13981 aagtcgcatt aactccgcag gcttcgcgga tagcgggtgc cacaatctcc gcccctccac
    14041 cgacaaccat cacccgcccg taacccgaaa aaaccgccag cgcgcggatc acgcgttgtt
    14101 tcagtgtttc ttccttttca cgaattaccg ccatcaggct ggcgtaatgc gcgtcattgt
    14161 ggatgtgctg gcgcagccag gcttcatcat ggcgatgttc gataatggta ttggcgatgt
    14221 ggtgactggt gcgcataccg ttagtggcca tcaccgacag tacggcatcg gccatcagag
    14281 aaacgcccac gtgtggatcg caaaacacct ggctgatacc tgccagttgc ccctgaacct
    14341 ttgccacatc cagcgtggtt ccgcccaaat ccacaatcag cagggattca aacggactca
    14401 tgtcagccag tgccttaaag ccagccggaa tggattcagg cataacctgt acgttacgga
    14461 tagtgaatgc ttcgccgttc tggtactcca ccgggcgcat gacgttcgct tttttg
//
LOCUS       DADLTD010000098        14492 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLTD010000098 DADLTD010000000
VERSION     DADLTD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.098"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..249)
                     /gene="grxB"
                     /locus_tag="JN701_002362"
     CDS             complement(<1..249)
                     /gene="grxB"
                     /locus_tag="JN701_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7364796.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRS"
     gene            complement(313..1521)
                     /gene="mdtH"
                     /locus_tag="JN701_002363"
     CDS             complement(313..1521)
                     /gene="mdtH"
                     /locus_tag="JN701_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY7364797.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            1757..2341
                     /gene="rimJ"
                     /locus_tag="JN701_002364"
     CDS             1757..2341
                     /gene="rimJ"
                     /locus_tag="JN701_002364"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7364798.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            2352..2999
                     /locus_tag="JN701_002365"
     CDS             2352..2999
                     /locus_tag="JN701_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY7364799.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            3001..3867
                     /locus_tag="JN701_002366"
     CDS             3001..3867
                     /locus_tag="JN701_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7364800.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            4034..5569
                     /gene="murJ"
                     /locus_tag="JN701_002367"
     CDS             4034..5569
                     /gene="murJ"
                     /locus_tag="JN701_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY7364801.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(5609..6025)
                     /gene="flgN"
                     /locus_tag="JN701_002368"
     CDS             complement(5609..6025)
                     /gene="flgN"
                     /locus_tag="JN701_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY7364802.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(6030..6329)
                     /gene="flgM"
                     /locus_tag="JN701_002369"
     CDS             complement(6030..6329)
                     /gene="flgM"
                     /locus_tag="JN701_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY7364803.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSVT
                     LSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEAQQDLQSN"
     gene            complement(6405..7064)
                     /gene="flgA"
                     /locus_tag="JN701_002370"
     CDS             complement(6405..7064)
                     /gene="flgA"
                     /locus_tag="JN701_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY7364804.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            7220..7549
                     /gene="flgB"
                     /locus_tag="JN701_002371"
     CDS             7220..7549
                     /gene="flgB"
                     /locus_tag="JN701_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY7364805.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            7553..7957
                     /gene="flgC"
                     /locus_tag="JN701_002372"
     CDS             7553..7957
                     /gene="flgC"
                     /locus_tag="JN701_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAY7364806.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            7969..8664
                     /gene="flgD"
                     /locus_tag="JN701_002373"
     CDS             7969..8664
                     /gene="flgD"
                     /locus_tag="JN701_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HAY7364807.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            8689..9897
                     /locus_tag="JN701_002374"
     CDS             8689..9897
                     /locus_tag="JN701_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HAY7364808.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGTNNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            9917..10672
                     /locus_tag="JN701_002375"
     CDS             9917..10672
                     /locus_tag="JN701_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HAY7364809.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            10810..11592
                     /gene="flgG"
                     /locus_tag="JN701_002376"
     CDS             10810..11592
                     /gene="flgG"
                     /locus_tag="JN701_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY7364810.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFLVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            11645..12343
                     /gene="flgH"
                     /locus_tag="JN701_002377"
     CDS             11645..12343
                     /gene="flgH"
                     /locus_tag="JN701_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY7364811.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            12355..13452
                     /locus_tag="JN701_002378"
     CDS             12355..13452
                     /locus_tag="JN701_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY7364812.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            13452..14393
                     /gene="flgJ"
                     /locus_tag="JN701_002379"
                     /pseudo
     CDS             13452..14393
                     /gene="flgJ"
                     /locus_tag="JN701_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
BASE COUNT         3457 a         3721 c         3950 g         3364 t
ORIGIN      
        1 ggaacgtttg ccggtcagta acggtttgcc gtcgagttta tcgacatagt gaacgatatc
       61 catgctttct ggcatatagc ggctgtcatc tttttgcaga atgggaacct gtttttgacc
      121 gaccatccgg gtgggtgttt ctgcgtcgtc gttgagcaga acatgtaatt cgacggggat
      181 atttttcagg ccgaaaatca tgcgggcttt gaggcagtaa gggcagtgat cgtaaatgta
      241 tagcttcacg tgactcctcc atttggctgt cagttcactt tcagtatgga ggagtcaggg
      301 gggagtatca aatcaggcgt cgcgttccag caaacgacgc gcggcgcgtt tctgactaaa
      361 ctgccaaccc agcgcaagga aagtgaagat gccaataatt cccagcatca tccacggaag
      421 ctctggctgg tgcgccgatt tgcccaggtc aaacagccag ccaccaccga tataaccaat
      481 agcgccgcca atcgccagac ccagacggct aaaccccata tagctgccgc gagctcttgc
      541 gtccgccagc gaagcactta aggtttcacg cgcaggctcg gcaatgatcg acccgatata
      601 aaacagacaa atcagggtga aaagttgttg caggccgctg accatgccca ccggcatcat
      661 gcttaatgac attatcaaca gcccagccat caaccggtgt tccagacgaa aatgcttttc
      721 actccagcgg gcgatagggt agagcaacgt taacgacaga cacgcttcaa tggcatacat
      781 ccatttaacg gcagagggcg cgccagccac gtcgttgacc ataattggca gcatcagcat
      841 cacttgtaca gccagcatgt agtaacccgc cagcgtcagg acataggtga caaaacgctt
      901 gtcacgcatc acgcgggtca tgccttcgcg aacgggcgtg cgtacggtgg agagtttcca
      961 tgccggtaac aaccacgcat tgaacgccgc acacagcaca aacaaaacgg cccctgtggc
     1021 gcagaccagg cgaaagtcgt attgcaacag ccagctcccc aacaatgcgc caatgaccgc
     1081 accggcactg tcctgcatca tcaacagcga gaaaaaacga ccacgttgtt gtggacggat
     1141 tagtttcacc accagcgccg aacgcggcgg atcaaacaac gtgccgccga gtcccgagag
     1201 caggcatgaa aaccacaata gccacggttc gtgggcgata cccattgtgg cgaatccggc
     1261 ggcgcgcatc agcataccgg taacaatcat cggtttggca ccaaagcggt cggcaattgc
     1321 gccgccgaaa atacccagac cttgctgaat aaactggcgt agaccgagag caataccgac
     1381 catgacggcg gcccagccca tttgatcaac gaagcggata gagatcagcg ggaagacaac
     1441 aaagaacccc agcacgacca gcatattatc gatgagcagg aaatatttac ccaggttcct
     1501 cgcctgcgat acgcgggaca tttcccctcc cgggaaataa aagatgagca ctttctattc
     1561 tgcattgccg ctaagacttt tcccatcact tcggggacaa tattttttta tcaaaacgtc
     1621 gctttgatag agagttttta tcaaatatgt gaataatgca gaaaatggca ttttggactt
     1681 ttcacagggt ctggttgcgc aggtatagta atacttacag cgtattaaag acgttacggg
     1741 aaggagtagg tatagaatgt ttggctatcg cagtaacgtg ccaaaagtgc gcttaaccac
     1801 agaccgactg gtcgtgcgtc tggtgcatga tcgtgatgcc tggcgtcttg cggattatta
     1861 cgcagagaat cgccatttcc tcaagccctg ggagccagtg cgcgacgaaa gccactgtta
     1921 tccatcaggc tggcaggcca ggctggggat gattaacgaa tttcataaac aaggttcagc
     1981 tttctacttt ggcttattcg acccggacga aaaagagatt attggcgttg ccaatttttc
     2041 caatgttgtt cgtggctctt ttcatgcctg ctatctcggt tattcgattg ggcaaaaatg
     2101 gcagggcaaa ggactcatgt ttgaagccct gaccgcagcc attcgttata tgcagcgcac
     2161 ccaacatatt catcgcatta tggctaatta tatgccgcac aataaacgca gcggtgattt
     2221 actggcgcga ctgggttttg aaaaagaagg ctatgcgaaa gactatctgt tgattgatgg
     2281 acaatggcgc gatcacgtac tgacggcatt aactacccca gactggacgc ccggccgcta
     2341 aggagaatct catgaaatat caacttactg cccttgaagc tcgcgtgatt ggttgcttgc
     2401 tggaaaagca ggtgaccacg ccggagcaat acccgctctc agtcaatggt gtagtcacgg
     2461 cctgtaatca gaaaacgaac cgtgaaccgg tcatgaatct gagtgaatct gaagtgcagg
     2521 agcaactgga taatctggtc aaacgccatt atctgcgcac ggtgagtggc tttggtaatc
     2581 gggtcaccaa atatgaacaa cgtttttgta attcagaatt tggcgatctg aaactgagcg
     2641 cagcggaagt ggcgttaatc accacgttgt tattgcgtgg tgcccagacg ccgggtgaac
     2701 tgcgcagccg cgccgcgcga atgtatgaat tcagcgatat ggcggaattg gagtcgacgc
     2761 tggaacaact ggcaaatcgc gaagatggtc cttttgtggt gcgtctggcc cgcgaaccgg
     2821 gtaaacgcga aagccgctac atgcatcttt tcagtggtga ggtagaggat cagccggcgg
     2881 taacggatat gtcgaacgcg gttgatggtg atttacaggc ccgcgtcgaa gccctggaaa
     2941 tcgaagtggc agaactgaaa cagcgtcttg attcgttgct ggcccatctg ggagattaaa
     3001 gtgaaaaaat tacgtatcgg cgttgtggga ttaggcggca ttgcgcaaaa agcgtggtta
     3061 ccggtgctgg cggcagcgtc tgactggatg ttacaaggag cctggtcgcc tacgcgcgcg
     3121 aaagccctgc caatttgtga aagctggcgc attccttatg ccgattcgtt atccagcctt
     3181 gccgccagtt gcgatgcggt ttttgtgcat tccagcaccg ccagccactt tgacgtggtc
     3241 agtacgttac tcaatgcggg tgtacatgtc tgtgtcgata aaccgctggc agaaaatctg
     3301 cgcgatgctg aacggctggt ggaactggcg gcgcggaaaa aactgacgtt gatggtcggt
     3361 tttaaccgtc gtttcgcacc actctacggt gagttaaaaa cgcaactcgc taccgcagct
     3421 tcgctaagaa tggataaaca tcgtagcaat agtgtcgggc cacacgatct ttatttcacg
     3481 ttgctggatg attatctgca tgtggtggat accgcgctgt ggttgtcggg cggcaaagcc
     3541 tctctggatg gcggtacgct actgactaac gacgctggcg aaatgctgtt tgccgagcac
     3601 catttttcgg ccggtccttt gcagatcacc acctgtatgc atcgccgtgc cggaagtcag
     3661 cgtgaaaccg tgcaggccgt gactgacggt gcgctcatcg acattacgga tatgcgcgaa
     3721 tggcgtgagg agcgcgggca gggcgtagtg cataaaccga ttcctggttg gcagagcact
     3781 cttgaacaac gtggatttgt cggctgtgcg cggcacttca ttgaatgtgt gcaaaatcag
     3841 acagttccgc aaaccgccgg cgaataggcc gtgctggcgc aacgtatcgt tgacaagatc
     3901 tggcgcgatg cgatgagtga ataaaccctg taacatctgg cggtagcaat tcaccataat
     3961 ccaggtagac tattcgcctc tttcagcgcc tgtcttgcag gcgttttgcc cgttggtctg
     4021 gattagaaca ccgatgaatt tattaaaatc gctggccgcc gtcagctcga tgaccatgtt
     4081 ttcgcgtgtg cttggcttcg cacgagacgc aattgtcgcc agaatctttg gcgcagggat
     4141 ggcaaccgac gcctttttcg tcgcttttaa acttcctaac ttgttacgcc gtatctttgc
     4201 cgaaggggca ttttcccagg catttgtacc gattctggcg gaatataaaa gtaagcaggg
     4261 tgaagacgcc acgcgggtct ttgtctctta tgtttctggc ctgctgacac ttgcgctggc
     4321 ggttgtgacg gtcgctggca tgctcgccgc accgtgggtg atcatggtga ccgcgccagg
     4381 cttcgctgat acagctgaca aatttgccct gaccagccag ctactaaaga ttacctttcc
     4441 ctatatcttg ctgatctccc tggcgtcgct ggtgggagcg attctgaata cgtggaaccg
     4501 cttctcgatt ccggcgtttg cgccaacact gcttaacatc agcatgattg gtttcgcgct
     4561 gtttgccgca ccgtacttta acccaccggt gctggcgctg gcgtgggctg ttacggtcgg
     4621 cggcatcctg cagctggtgt atcagctacc gcacctgaag aagatcggca tgctggtcct
     4681 gccgcgcatt aacttccacg atgccggagc aatgcgcgtg gtgaaacaga tgggaccggc
     4741 gatccttggc gtctctgtga gccagatctc cttaatcatc aacaccattt ttgcctcgtt
     4801 tcttgcttcc ggttcggtgt cttggatgta ttacgccgac cgcttaatgg agtttccgtc
     4861 cggtgtgctg ggcgtggcgc ttggcaccat tttgctgccg tcgctgtcga aaagttttgc
     4921 cagtggcaat catgatgaat acaaccgttt gatggactgg gggttgcgtc tttgtttcct
     4981 gttggcgctg ccgagtgcgg ttgcgttggg cattctttca ggtccgttaa ccgtttcgct
     5041 gttccagtac ggtaaattta ccgcgtttga tgcgctgatg acccagcggg cgttaattgc
     5101 ctactcggtg ggtttgattg gcctgattgt agtgaaagtg ttggctcctg gcttttattc
     5161 ccgccaggac attaaaacgc cagtgaaaat tgccatcgtt acgctgattt taacgcaatt
     5221 gatgaacctg gcgtttattg gtccgttgaa acatgccggg ctgtcacttt ctattggtct
     5281 ggcggcgtgt ctgaatgctt cgctgcttta ctggcagttg cgtaagcaga aaatctttac
     5341 tccgcaaccc ggctggatgg cgtttctgtt gcgtctggtg gtggcggtac tggtgatgtc
     5401 tggcgtgctt ttaggtatgt tacatatcat gccggagtgg tcattgggta ccatgccctg
     5461 gcgcttactg cgtttaatgg cggtcgtgct ggcggggatt gccgcgtact tcgctgcact
     5521 ggcggtactg ggcttcaaag ttaaagaatt tgcccgccgg acggtgtaac aatgcattcc
     5581 ggcctgcagt gcaggccgga gataatcttc agatcgaaat ctttttaccg ccgcgatggg
     5641 ttgttgaggt ctgaccgttc gccccatata gcgtcggttc ctgatgcggt ttcaacattt
     5701 ccagcgcctg ttgattgcgc tcaatctgtc cttccagtaa ccaaccgtta tgttgattca
     5761 tttggcgtag ttgctgcgtt ttcacagtaa tttcctgcca gcgttgacta atatcaacgc
     5821 tatttgctgt attgggttct ttcttgcgta actgttcgag gtaatccagc gtcgccagca
     5881 gtgagctttt ttgttctgta atccattgca actggctgcc gttgatctgc cccatagaga
     5941 gatgttgctg ctcttgatcc attaccgttt tgagatcgtt aagcacagcg gacatctggt
     6001 caaggatctc tgcaagacgt gtcatacgat cagttactct gcaagtcttg ctgagcttcg
     6061 ttgatcagcg catcggcaat tttgccgatg tccattttta gttcaccgtt acgaatcgcc
     6121 agttttaacg cttcgacacg ttcaagattg atatcactgc tgccgggttg catcagtttt
     6181 gcttgcgcgt cgcttaacgt cacactggtg ctggtgctgg tggagtcggt tgtttttgcc
     6241 gcccgggtgt tcgttaccgg cgcgtcagtg gtttcgcgcg gttgaacggt gcttacggga
     6301 ttcagaggcg aagtgcgatc aatactcatg gtttattcct cattgagggc gcttttatca
     6361 tgtgttgctt atttatcggc aagggacggg taatctttaa cagcttacag gtttataaga
     6421 atattcccat ctgcatcaac aacgccgctg accaccagtc ccgataccat gcgcatccgc
     6481 gcattctgtg cgacggctgc attgttcagc gcctgacctt ctgcgttggc gctaaacccg
     6541 tcaccgctgg cgatcacgtt gacgcgttgt cccgctttta cccgccatgc ctggcgaaaa
     6601 tgggttaatt ggattggttg atcgggtgat agatcgcgta ggctaatggc atcaacaagt
     6661 tgattgatat ccagcaccgt acgcggtggc agggtatcca gtcgtccgcg ttttagtttg
     6721 acattgccag cttccagctt tcctccccgc acaatgggca tcgcggcaac cacataattt
     6781 cctgtggcct gcacattaac ctgtaaatat cgtttgtcgt taccgcagcg tgccaacaca
     6841 ttcacattgc cccacaggcg ggaattattg ctcatcgaaa gcaatggctg ctcgcacggc
     6901 ggtagtagat tgggtgctgt acgaatagaa acacgaacct catcacttac ccccacgagc
     6961 tgggcgctaa aaaaggtgtg caattgcgat gtgagattgc tcgccgcact taacggactg
     7021 aacagtatcg cgatgatcgc cacgctacgt tttattgcca gcattttcgc cccccagcta
     7081 tttctacaac gtgaattgta cccgtcccca atggtcatca acggcataaa tagcgaccca
     7141 ttttgcgttt attccgccga taacgcgcgc gtaaaggcat ttaagctgat ggcagaattt
     7201 tgatacctgc ggaggagata tgctcgataa gctcgacgcc gccttacgtt ttcaacaaga
     7261 ggcgctcaat ctgcgcgccc agcgtcagga agtgctggca gcaaacatcg ccaatgccga
     7321 tacccttggt tatcaggcgc gcgatatcga ttttgccagt gaacttaaaa aagtcatgca
     7381 acgtggacgg gatgcaacca gtgtggttgc tctgacgatg acctcaacgc aacacattcc
     7441 ggcgcaggcg ctgacgcctc ctaccgcaga actgcaatac cagatgagcc ttagtgcgtt
     7501 gagcgggcaa atcaaaggca tgatgaacgt tttacagagc ggaaattaac ggatggcact
     7561 gctgaatatt tttgatatcg ccgggtcggc gttaactgcc cagtcccagc gcctgaacgt
     7621 ggcggccagt aatctggcga atgctgatag cgtgaccggt cccgatggac agccatatcg
     7681 ggcaaaacag gtggtattcc aggttaacgc tgcaccaggt gctgcgacag gcggcgtaaa
     7741 ggttgccgat gttatagaaa gtcaggcccc ggacaaactg gtttatgaac cgggtaatcc
     7801 gctggcggat gcaaagggct acgtaaaaat gccgaacgtt gatgttgtcg gagagatggt
     7861 taacaccatg tcggcgtcac gcagctatca ggccaatgtt gaagtgctca acacggtgaa
     7921 aagcatgatg ctgaaaaccc ttacgctcgg tcaataaagg agaaagctat gtccattgcg
     7981 gtaaccacca ccgatccgac aaataccggc gtcagtacca ccagcagtag ttcgctcacg
     8041 ggcagcaacg ccgcagattt acaaagcagt tttctgactt tgctggtggc gcagctgaaa
     8101 aaccaggacc cgaccaatcc aatggaaaac aacgagctga cgtcgcaatt ggcacaaatc
     8161 agcacggtca gcgggattga aaaactcaat accatgctcg gatctatttc cggacagatt
     8221 gataacagcc agtcgttaca ggccagtaac ctgatcggtc acggcgtgat gatccccggc
     8281 acaactgttc ttgcgggaac cggcagtgaa gaaggggctg tgaccacgac cacgccgttt
     8341 ggtgttgagc tgcaacaggc ggcagacaaa gttacggcca ccatcaccga taaaaatggc
     8401 gcggttgtgc gcaccattga tattggtgaa ctgaccgccg gagttcacag tttcacctgg
     8461 gacggtacgt tgactgatgg cagcactgcg ccgaacggtt cttacaatgt agcgattacc
     8521 gccagtaacg gtggtacaca actggttgcc cagccgctgc agtttgctct ggtgcagggt
     8581 gtgatccgcg gcaacagcgg taatacgctg gatctcggca cttacggcac caccaccctc
     8641 gacgaagtac ggcagataat ttaagccttc acatttcagg agtcagtcat ggccttttct
     8701 caagcggtta gcggattaaa cgctgccgcc actaacctcg atgttattgg caacaatatt
     8761 gccaactccg ccacctacgg ctttaaatca ggcacggcct cttttgccga tatgtttgcc
     8821 ggttcgaaag tgggactggg ggtaaaagtt gccggtatca ctcaggactt tacagatggc
     8881 acgaccacca acaccgggcg aggtctggac gttgctatca gccagaacgg ttttttccgt
     8941 ctggtagaca gcaacggttc ggtgttctac agccgtaacg gacaatttaa gctggatgaa
     9001 aaccgtaacc tggtgaatat gcaaggttta cagctgacgg gttacccggc aaccggtacg
     9061 ccgccgacta ttcagcaagg ggcgaatccg accaatattt cgatcccgaa taccctgatg
     9121 gcagcgaaaa ctaccaccac ggcgtcgatg cagatcaacc tgaattccag tgatccgctt
     9181 cctactgtta cgccattcag cgccagcaat gcggatagct ataacaaaaa aggttcggtg
     9241 actgttttcg acagtcaggg taatgctcat gacatgagcg tctactttgt gaagaccggg
     9301 gataataact ggcaggtcta cacccaggat agcagtgatc caaacagcat tgcgaagaca
     9361 gcgacaacac tggaatttaa tgctaatggc acattagtgg atggtgcgat ggcgaataat
     9421 atcgcaaccg gcgcaattaa cggtgcagaa cccgccacgt ttagtctgag cttcctcaac
     9481 tccatgcagc aaaataccgg cactaacaat attgtggcaa ccccccagaa cggctacaaa
     9541 ccgggcgatc tggtgagtta tcaaatcaat gatgacggta cggttgtcgg caactattcc
     9601 aacgaacaaa ctcaactgct ggggcagatt gtactggcga actttgccaa caacgaaggt
     9661 ctggcatccg aaggcgacaa cgtctggtct gcgacgcaat cttctggcgt ggcgctgttg
     9721 gggacagccg ggacgggaaa ctttggcacc ctgaccaacg gtgcgctgga agcgtccaac
     9781 gtcgatctca gtaaagaact ggtcaatatg atcgttgccc agcgtaacta tcagtctaac
     9841 gcccagacca tcaaaaccca ggaccagatc ctcaacacgc tggttaactt acgctaatcg
     9901 ctgacgggat agctcaatgg atcacgcaat ttatactgcg atgggagcag ccagccagac
     9961 gctgaatcaa caggcggtaa ccgccagtaa tctggccaat gcctctacgc ccggttttcg
    10021 cgcgcagtta aacgccttgc gcgcagtgcc ggtggaaggg ctttcactgc ccacgcgcac
    10081 gctggtaacg gcgtcaacac cgggtgcaga catgacgccc ggcaagatag attacacctc
    10141 gcgcccgctg gacgttgcgt tgcagcagga tggctggctg gccgtgcaga ccgctgacgg
    10201 cagcgaaggg tatacgcgta atggcagcat tcaggttgat cccaccgggc aactgacaat
    10261 tcaggggcat caggtgatag gcgaggcggg gccaattgct gtgccggaag gagcggaaat
    10321 cactattgct gccgatggca caatctcggc gctcaatccg ggcgatccgg caaatacagt
    10381 tgcgccagta gggcgtctga aactggtgaa agccacgggc agcgaagtgc agcgcggtga
    10441 cgacggcatt tttcgtttaa gcgcagaaac ccaggccacg cgagggccgg tactgcaggc
    10501 agatccaacc ttgcgtgtga tgtcgggggt tctggaaggc agtaacgtca atgccgttgc
    10561 ggcaatgagc gacatgattg ccagcgcgcg gcgttttgaa atgcagatga aggtgatcag
    10621 cagcgtcgat gataacgcag gccgtgccaa ccaactgctg tcgatgagtt aattgaacga
    10681 ttgatgacat tgcaataaaa gcgcgcggac tattccctcg cccctttggg gagagggtta
    10741 gagtgagggg gaattcacac tgtcgcgtgc ttctgctctg cttgtcacta atccactaca
    10801 ggacatttta tgatcagttc attatggatc gccaaaacgg gccttgacgc ccagcaaacc
    10861 aatatggacg tcattgccaa caacctggca aacgtcagta ctaacggttt taagcgtcag
    10921 cgcgcggtgt ttgaagatct gctttatcaa accattcgcc agccgggggc acagtcttcc
    10981 gaacaaacca ccttaccctc cggattacaa atcggcacgg gggtacgccc ggtcgccact
    11041 gaacgcttac acagccaggg aaatctgtcg cagaccaaca acagcaaaga tgtcgcgatt
    11101 aaagggcagg gctttttcca ggtgatgttg ccagatggtt catcagccta tacccgtgac
    11161 ggctctttcc tggtggatca gaacgggcag ctggtgacgg ctggtggttt tcaggtgcag
    11221 ccagcgatca ccattccggc gaatgcgtta agtatcacca tcggtcgtga tggcgtggtc
    11281 agcgtaaccc aacaaggcca ggcagctccg gttcaggttg ggcagctcaa tctcaccacc
    11341 tttatgaatg acaccgggct ggagagcatt ggcgaaaacc tctacaccga aacgcaatcc
    11401 tctggtgcac cgaacgaaag cacgccgggc ctgaacggcg cgggattgct gtatcaaggg
    11461 tatgttgaaa cgtctaacgt caacgtggcg gaagaactgg tcaatatgat tcaggtgcaa
    11521 cgcgcttacg aaatcaacag taaagcggtg tccaccaccg atcagatgca gcaaaaactg
    11581 acgcaactct aaggcttaac cggtggcagg ttcaccggtt tactgatttt tgaagatgat
    11641 agccatgcaa aaaaacgctg cgcatactta tgccatttcc agcttgttgg tgctttcact
    11701 aaccggctgc gcctggatac cctccacgcc gctggtgcag ggggcgacca gtgcacaacc
    11761 ggttcccggt ccgacgcccg tcgccaacgg ttctattttc cagtctgctc agccgattaa
    11821 ctatggctat caaccgctgt ttgaagatcg tcgaccacgc aatattggcg atacgctgac
    11881 catcgtgttg caggagaacg tcagcgccag caaaagctcc tctgcgaatg ccagccgtga
    11941 cggtaaaact aattttggct ttgatactgt gccgcgctat ttgcaggggc tgtttggtaa
    12001 cgctcgtgcc gatgtcgaag cctccggtgg taacacgttc aacggaaagg gcggggccaa
    12061 tgccagcaat acctttagcg gcacgttgac ggtgacggtt gaccaggtac tggtcaacgg
    12121 caacctgcat gtggtgggtg aaaaacagat tgccattaat cagggtaccg aatttattcg
    12181 cttctctggc gtggttaatc cacgcactat cagcggcagc aataccgtac cgtctactca
    12241 ggtggtggat gcgcgcattg aatacgtagg caatggctac attaacgaag cgcaaaatat
    12301 gggctggttg cagcgtttct tccttaacct gtcgccaatg taagtgaggc tgtggtgatt
    12361 aaatttctct ctgcattaat tcttctactg gtcacgacgg cggctcaggc tgagcgtatt
    12421 cgcgatctca ccagtgttca gggggtaagg caaaactcac tgattggcta tggtctggtg
    12481 gtggggctgg atggcaccgg tgaccagaca acccagacgc cgtttaccac acaaacgctt
    12541 aataacatgc tctcacagct gggaattacc gttccgacgg gcaccaatat gcagctaaaa
    12601 aacgtcgctg cggtaatggt gacagcgtca cttcctccgt ttggacgtca ggggcaaacc
    12661 atcgatgtgg tggtttcttc catgggtaat gccaaaagct tgcgtggagg tacgttgttg
    12721 atgacaccgc ttaagggcgt tgacagtcag gtgtatgcgc tggcgcaggg caatattctg
    12781 gttggcggcg caggagcctc cgctggcggt agcagtgttc aggttaacca actgaacggt
    12841 ggacggatca ccaatggtgc ggttattgaa cgtgaattgc ccagccagtt tggcgtcggg
    12901 aataccctta atttgcaact taacgacgaa gatttcagca tggcgcagca aatcgctgac
    12961 accatcaacc gcgtgcgtgg atatggcagc gccaccgcgt tagatgcgcg gactattcag
    13021 gtgcgcgtac cgagtggcaa cagttcccag gtccgcttcc ttgccgatat ccagaatatg
    13081 caggttaatg tcaccccgca ggacgctaaa gtagtgatta actcgcgcac cggttcggtg
    13141 gtgatgaatc gcgaagtgac cctcgacagc tgcgcggtag cgcaggggaa tctctcagta
    13201 acagttaatc gtcaggccaa tgtcagccag ccagatacac cgtttggtgg tggacagact
    13261 gtggttactc cacaaacgca gatcgattta cgccagagcg gcggttcgct gcaaagcgta
    13321 cgttccagcg ccaggctcaa taacgtggtg cgcgcgctca atgcgctggg cgctacgccg
    13381 atggatctga tgtccatact gcaatcaatg caaagtgcgg gatgtctgcg ggcaaaactg
    13441 gaaatcatct gatgatcagc gatagcaaac tactggcaag tgcggcctgg gatgcgcaat
    13501 cactcaacga actaaaggcg aaagcgggcg aagatccggc ggcaaatatc cgtccggtgg
    13561 cccgtcaggt ggaagggatg ttcgtgcaga tgatgttgaa aagcatgcgc gacgctttac
    13621 caaaagatgg cctgttcagc agcgagcaca ctcgcctgta taccagtatg tatgaccagc
    13681 agattgccca acagatgacg gcgggcaaag gtctggggct tgcagagatg atggttaaac
    13741 agatgacgcc agaacaacca ttgccagagg agtccacgcc agcagcaccg atgaaattcc
    13801 cgctcgaaac tgtggtgcgt tatcaaaatc aggcgctttc gcagctggtg caaaaggccg
    13861 tgccacgtaa ctacgatgat tcgctgccgg gtgacagtaa agcattcctc gcgcaactct
    13921 cgctgcccgc ccaactggca agccagcaaa gcggtgtgcc acatcatttg atcctcgctc
    13981 aggcggcgct ggaatctggt tgggggcaac ggcaaatccg ccgcgaaaac ggcgagccga
    14041 gctataacct gtttggtgtc aaagcctctg gcaactggaa agggccagtt actgaaatca
    14101 ccacgactga atatgaaaac ggcgaagcga agaaagtaaa agcgaagttt cgggtctaca
    14161 gctcgtatct ggaagcatag tcggattacg ttgggctgtt aacgcgtaac ccgcgctacg
    14221 ccgccgtgac caccgccacg agtgcggagc agggggcgca ggccctacag gacgcgggct
    14281 atgccaccga tcctcactat gcccgcaaac tcaccaacat gattcagcag atgaaatcga
    14341 taagcgacaa ggtgagcaaa acctacagta tgaacattga taatctgttt tgaataactc
    14401 aagtccggcg ggtcgctgcc gataatactc tgtaattgaa ggcttataag gaacctccat
    14461 gtccagcttg attaataacg ccatgagcgg ac
//
LOCUS       DADLTD010000099        14435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLTD010000099 DADLTD010000000
VERSION     DADLTD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.099"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..789)
                     /gene="fimH"
                     /locus_tag="JN701_002380"
     CDS             complement(<1..789)
                     /gene="fimH"
                     /locus_tag="JN701_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY7364813.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGA"
     gene            complement(809..1312)
                     /gene="fimG"
                     /locus_tag="JN701_002381"
     CDS             complement(809..1312)
                     /gene="fimG"
                     /locus_tag="JN701_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY7364814.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1325..1855)
                     /gene="fimF"
                     /locus_tag="JN701_002382"
     CDS             complement(1325..1855)
                     /gene="fimF"
                     /locus_tag="JN701_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY7364815.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1865..4501)
                     /gene="fimD"
                     /locus_tag="JN701_002383"
                     /pseudo
     CDS             complement(1865..4501)
                     /gene="fimD"
                     /locus_tag="JN701_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
     gene            complement(4568..5293)
                     /gene="fimC"
                     /locus_tag="JN701_002384"
     CDS             complement(4568..5293)
                     /gene="fimC"
                     /locus_tag="JN701_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7364816.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5330..5869)
                     /locus_tag="JN701_002385"
     CDS             complement(5330..5869)
                     /locus_tag="JN701_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7364817.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(5934..6482)
                     /gene="fimA"
                     /locus_tag="JN701_002386"
     CDS             complement(5934..6482)
                     /gene="fimA"
                     /locus_tag="JN701_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/fibrin-binding adhesin FimA"
                     /protein_id="HAY7364818.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(6963..7559)
                     /gene="fimE"
                     /locus_tag="JN701_002387"
     CDS             complement(6963..7559)
                     /gene="fimE"
                     /locus_tag="JN701_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY7364819.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(8037..8639)
                     /gene="fimB"
                     /locus_tag="JN701_002388"
     CDS             complement(8037..8639)
                     /gene="fimB"
                     /locus_tag="JN701_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HAY7364820.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10095..10811
                     /gene="nanC"
                     /locus_tag="JN701_002389"
     CDS             10095..10811
                     /gene="nanC"
                     /locus_tag="JN701_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY7364821.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10831..11937
                     /locus_tag="JN701_002390"
     CDS             10831..11937
                     /locus_tag="JN701_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY7364822.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYTPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            12002..12982
                     /locus_tag="JN701_002391"
     CDS             12002..12982
                     /locus_tag="JN701_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY7364823.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAKEDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWREK"
     gene            complement(12990..13112)
                     /locus_tag="JN701_002392"
     CDS             complement(12990..13112)
                     /locus_tag="JN701_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7364824.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13194..14066
                     /locus_tag="JN701_002393"
     CDS             13194..14066
                     /locus_tag="JN701_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7364825.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            14111..14290
                     /locus_tag="JN701_002394"
     CDS             14111..14290
                     /locus_tag="JN701_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364826.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         4045 a         3329 c         3151 g         3910 t
ORIGIN      
        1 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
       61 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
      121 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
      181 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
      241 atcgcagccg ccagtaggca ccaccacatc attattggcg taaatattcc acacaaactg
      301 gaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgagcc
      361 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
      421 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
      481 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccataagc
      541 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
      601 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
      661 tacataaaca ttagcgctgc caccgccaat agggatagca gtaccattgg cggttttaca
      721 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaataac
      781 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
      841 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
      901 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggtttcgcca gtattcaatg
      961 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     1021 aatatccggt actgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     1081 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacatca
     1141 gactgaaaga ataaagatcg ccgagatcaa ccgtggcatt ggtggtggaa actgtgcacg
     1201 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt attgtcgcac
     1261 ttgcgagcgc caatattgcc gccaataaat acccacgttt gcgccatttc atgagcatct
     1321 ccagttactg atattcaaga gtgaaggtag ccgtggcatt gatatgcccc gcagtgacag
     1381 gcacctgtgt cgccattagc cgggcgtaaa aattcaacgt atttggttta cccggcgtca
     1441 gggtcgtcca cgaaatcgcg gacgatggag cattaagggg tatctgattt tgctgctcat
     1501 tcagaagctg tattcccagt cccgaagccg ctgacaccgt attttcaagt gcaagcaggt
     1561 tggcattgtg gctatctgca acgccggtaa acccaacctt tacggcagaa acggcgttac
     1621 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgttgcgcca atgttgttaa
     1681 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     1741 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     1801 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     1861 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     1921 gttgataatt ggcgatacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     1981 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     2041 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgggttagcg
     2101 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     2161 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccagg gtattggtat
     2221 ccagcgccac tctgttttcc cgatattcgg tggcataagg cagcacggca taaccgcgcc
     2281 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     2341 caagtaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccagta
     2401 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     2461 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     2521 catcgcctcc cccggcatag ccggtttgca cgctatagct gaggttgttg tcttccagca
     2581 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacacgctgt
     2641 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     2701 gaatattgac gttaagcgct aacatctgat cccgcccttt ttgccaggcg tttttcgtca
     2761 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctagaatt
     2821 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
     2881 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
     2941 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
     3001 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
     3061 aacggtaacc cactaactga atattcgtgc ctgactcatt gagcgatttg ttatagagaa
     3121 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
     3181 tatccacaga cagagcgccc agtgccccca tgtttttccc gataccgaaa ttaaaagcac
     3241 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
     3301 gtaatgtact ttggaaaaag cggggttttt cctgctgcgt atttccacta cggtattctc
     3361 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
     3421 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
     3481 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
     3541 aaatgtcata cccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
     3601 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
     3661 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccagcgtca
     3721 gccgggaacg taacggtatt atgtctcgct caagccaggt attgatatgc tgccatttat
     3781 ttttgctacc tgatgatctg tcgctactgt tataactcca gctggtattg tcgcgtaaac
     3841 accacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
     3901 caatctgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
     3961 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
     4021 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcatggatca
     4081 ttgaggttaa tggcacgcag gcatcatctg ccagcagatt cataccggag acagaagccg
     4141 cattcagccc catactggca agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
     4201 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
     4261 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
     4321 cagcctgggg atcatccgct aaaaagcgcg gattaaaata gagttcggca gatgacaaag
     4381 gtgcctgtgc ggcaaaagca caggcgacaa cgagccggac aaaaaaacca gccaaacgat
     4441 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
     4501 ttattcctcc ggtttaaaat tacccggcag tcaattcttt ttattcaggc gagaaattcc
     4561 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
     4621 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
     4681 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcagat
     4741 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
     4801 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
     4861 tgataattgc gaactgtagc gtattttcag tcaattttga tttatccatt gacggaatcg
     4921 ctttaacatt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
     4981 caagaatacg caaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
     5041 taaaacgacc atcctttaca ccatcggcat tttccatcca tgattgaatt aaataggtac
     5101 tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta
     5161 cgcgagtcgc acctaaggcc actcccgctt cagcgcgtcc ggcaaccatc attgccatga
     5221 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattggcgt
     5281 ttttattact cactgtcctg ttcctgttat cacattatct gctgaacaat tactgatagg
     5341 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccaacgc ccggtagcac
     5401 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttactg
     5461 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctacg ccaatattgg
     5521 tggctatccc tggcccctct cccacggaaa gcacatccgg atttttacca tccgcgacac
     5581 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
     5641 caaaaggcac cggtgcgcta tcttccccta ccgcatgaaa ccggttactg ctgatttgcc
     5701 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
     5761 cgccctgaaa ttgcatattt ccgccgggca gcgtagtatt ccatttattt ccggccagag
     5821 caaacattgg cagcaacgac gctaatagaa ataatctctt ccttttcatc gttattttta
     5881 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtccct gaacctgggt aggttattga
     5941 tactgaacct tgaaggtcgc atccgcatta gctgcgccag gggttgcgac accggttgca
     6001 taataacgcg cctggaacgg aatggtgtta gtaccgttat tcagggttgt ttgctcactg
     6061 aatgtcgcac catccagcgt cagcgcagca cccgttctgt ccaggatctg tacaccaacg
     6121 tttgttgcgc tacccgcagc tgaactctgc agagccagaa cgttggtatg acccgcatca
     6181 atcgccgtac ctaaaaaggc aactgcggct ttagatgcaa cattggtatc gcaatcattc
     6241 agctgaatgt taaaaccgac agcagaactg gttgctcctt cctgtgccag cgatgcggta
     6301 cgaacctgtc ctaactgaac gatttgatca acagagcctg catcaactgc gcaagcggcg
     6361 ttaacaactt cccctttaaa gtgaacggtc ccaccattaa ccgtcgtggc agcggccaga
     6421 gccgctgtag aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
     6481 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
     6541 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
     6601 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
     6661 ttggcttaat attctattgt tatctttatt tatagatttt tatattgcat gaggtggttt
     6721 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
     6781 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaacag ttagatgatt
     6841 tttgtcgttt tttaaaattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
     6901 acaatttggc cccaattgtc ttgtatttat ttatttttta actttattat caataagtta
     6961 attcaaacct cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg
     7021 gcaaaacgag cagcattact ggcggtataa cgtacagtat ggcgaatatt tcgatgcccg
     7081 agataatcct gaattaaacg agtatctgca ccacgctccg ccagttcata accgcaagca
     7141 tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcacga
     7201 ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatggcg
     7261 tcggtccggt cagcgccttt ccagttagca cgttccaggg tccagcgttc cacagcttca
     7321 cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt
     7381 attcgacctt cattaaggtc aagatcctga taatgcagat caagcagttc actaatacgc
     7441 atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa
     7501 caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca
     7561 atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt
     7621 caaaattagc ccaatagata aaaagataac cacacacgaa gtgatgtggc tatcagtcaa
     7681 ttacattaaa tatcatacaa aaataaaata tcactgatgt gacaacatct attttcattg
     7741 atttgattca atataacaat agattgtagt agattagctt ccagttgctt aaaaaataaa
     7801 gaaaaaagtg cgaaaacagg agaatttaag tgatgtgtaa atttatgaat gattttgttt
     7861 atttattttg aagaacataa accattgtat tttgtatgga tatggttaac ttaatgctta
     7921 ttatgataat taagaattat acttacaata acatcaatta ataaatggtt aatcattttt
     7981 gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat
     8041 aaaacagcgt gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca
     8101 ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata atcctggata
     8161 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatggcg taacatgtgc
     8221 ggatgaatct ccagtgacaa cccggcatta ccaccggaag tcgagataat atggtaaaac
     8281 tgttgccgag aaagcggatt ccccttacgc gataaaaata cccactcgct ctcagcatgc
     8341 gggtacgaag tacggatact caaccagttt tttaaagcct gaacttcttt attcaatagc
     8401 gggggcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgccttaaga
     8461 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
     8521 agcaaagtca gacaataatt acgtgctgca tgaggcccgg tatttgctgc tttaaggagt
     8581 gattcgattt cactatgggt caggaagttc ctttttttgt tatcagcctt attcttcatc
     8641 gtttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat aatccaacat
     8701 ttttatcctg attatgttta taaaagcgaa cgtttgctta ataactaacg taagtggacc
     8761 agttcttctg agtgaactta aatggggtag caacagttaa ttataataac gttgccatac
     8821 aaacgctatg ctcttgcatg ctatgtacct ttatatattt atcaatcgga tacgaatcta
     8881 ataatacccc tcatcattaa tgcaactaat cttatctata tatcatgtga tatgttctgt
     8941 aacaagtaaa atcagcataa aaaattcagc aaaatttaat attcgttcaa ctagccgtct
     9001 cacatatgac attattagtc aactccattt gccacaaaat ggctatttca tggaacgcag
     9061 taatttctta gtggctatta tcatgctaat tcttaggtcc ctagcgatta ttcctggctg
     9121 attcgtcagc ttacgccatg agattgttac attccttttt atcctggaga tatgggggaa
     9181 cgcaggagat gattttttca tctacattcg ttggtaaata tagctttggt cactacgact
     9241 taaaccttcc tcaatcacag ccattcgccg ggcgatacgg caaacaaact aagtgaaaca
     9301 tcatcctgaa aacaccaaca tcaacaagcc tctccagatc gacttcagaa gtgaccagtt
     9361 acaagccaca aaccaggaaa catatttcat gatgagaatt atgctcagcc tgatggcggg
     9421 taaacactga ttttcaccaa gctcactgag ttagcttgca aagcttccct tgtttagact
     9481 ccaaataaat tatttataaa acaaacattt aaacaaagat ctttagtttt tgaagaccac
     9541 cgcaagtgtt cgtctggctt cacatggcat cttcctctta gaaaaagatc gacatatttt
     9601 gtgacacgaa ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata
     9661 acaggtataa aggtgcacca agatagtcaa tgagacaggg catctcgcaa tctatggcaa
     9721 acatcacttc agttctttct catcgggtga tgaaaacgca cttcagtctg aaaggaatat
     9781 gaaaatgaga tcaacagaca ctctatttta tgactctggg taaaatggat tgagtaagtg
     9841 atatagctta cgaaaattca aatcaattaa acatcagaag agattttata ctcaggtatt
     9901 taatctggat ctctgtttat ttaaataatg tgaaaagaga tttttcacag gagaccttat
     9961 acaaaaaaat ataaaataca gctaccggtt gccaaagaca ctataagcct ggcaaaaaat
    10021 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
    10081 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
    10141 cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa
    10201 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
    10261 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
    10321 atgatgttca ggttgaagtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc
    10381 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
    10441 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
    10501 accgacaaca agacttatcc agtgatatgt ctcgtgataa cgttcatcgt tgggatggat
    10561 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
    10621 aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc
    10681 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgtc
    10741 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
    10801 ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc
    10861 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
    10921 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg
    10981 gcatggtaca agctggatac acaggccaaa gataaaaaat ggacagcgtt agctgcattc
    11041 cctggtggac caagagatca agcaacctct gcatttattg atggcaatct gtatgtgttt
    11101 ggcggcattg gcaaaaacag agagggcttg actcaggtat ttaatgacgt acacaaatac
    11161 acccccaaaa ccaatagctg ggttaaattg atgtcgcacg caccgatggg catggcgggt
    11221 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
    11281 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tgtagataaa
    11341 atcaatgcct actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
    11401 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
    11461 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
    11521 cccggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
    11581 aataagcttg ctcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
    11641 aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag
    11701 cacggaaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
    11761 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
    11821 tcattgccct ggaataatag tctattgatt attggcggtg aaactgcagg cggcaaagcg
    11881 gtgacggatt cagttttgat ctctgtgaag gataataaag tcacagtaca aaactaacgc
    11941 ttcagggccc cagtaaaggg gccctgctat caatttatca tttcaattac gcgaaaatta
    12001 tatggacgca ataatatcgc ccgattatta ctatgttctt accattgctg gtcagtctaa
    12061 tgctatggcg tatggcgaag gactgccatt accggccaag gaagatgcgc ctcatcccag
    12121 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa
    12181 cgacattatt ccactaactc actgcccaca cgatgttcag gatatgcagg gttatcacca
    12241 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
    12301 acggaaatta ctgtccttta ttcctgataa cgcaggggtt ctcatcgttc cgtgttgccg
    12361 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
    12421 ccatgatgct tgtcgttggg gaacggatac tccgctatac caggatttag tcagcagaac
    12481 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg
    12541 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt
    12601 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
    12661 gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga
    12721 agctatttat ggcaattatc aaaataatgt tttagctaat attattttcg tcgacttcca
    12781 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaatcat
    12841 gggatattac ggttcagcgt accgctcacc ggagaactgg acgacggcac tgcgaagcag
    12901 tcatttcagc gcggcagccc gtcgggggat tatttctgac aggtttgtag aagcaatttt
    12961 gcagttttgg cgcgaaaagt gagcgttcat taatttgatt tattcgcatc catcaacgct
    13021 tttaactcct ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg gcaattaggg
    13081 cacacaagca gcaaaaaaac aaatgagatc aaggtaatat atcttcactt gcaggcttga
    13141 acagcagaaa atttgcgagc atgatcgcaa ttatttcact gctagggata tcaatggctt
    13201 ccagtaaatc gctacagcag gcaattgcca atataaaaat ctggcataag ggtgaacagc
    13261 gcgcaccgca taaaccattg ttattgctat acgtattagc gggatacctg aatggacatc
    13321 cacgcctttt cgattatgga tcggaaatct acgaacctct gcacagttta ctggaacgtt
    13381 ttgggccaca gcgttcacag taccgacctg atatgccgtt ctggcgatta caaggcgacg
    13441 gattctggca attacacaat gccgagcttt gctcaacagc agggagcagc cgacaaccgc
    13501 cagtgaagga actgaacgaa taccacgttg caggcggttt cgatgaacag cactacgccc
    13561 tggtaaccgg caataaaaaa ctcatcaata ctctagcgca acagatactc gaagcgcact
    13621 tcaccgagag tattcaggaa gaaattgctg acgagctggg ttttgatctc cagcaaatcc
    13681 gtaaacagcg cgacccgtta tttcgcaaaa atgtattacg cgcttataac taccagtgcg
    13741 cgatatgtgg ctttaatatg cgccacgacg acaccaccgt cgcgctggaa gcggcgcata
    13801 taaaatggaa acagcacggc ggtccttgtg aaattcctaa tggcctggcg ctttgcgcaa
    13861 ttcaccataa agcctttgat aaaggatcga ttggcctgga cgaaaatatg cgggtactgg
    13921 tatcagatgc agtaaacggc ggagggagcg ttgagagatt attctgggat tttgacggga
    13981 aaattattgc gttgccgcag gtgcgtaaaa attaccctta tgaggggttt gtggagtggc
    14041 atcggaatga ggtttttagg gggtgaggtt gataactatc gcagtatgtt caatattgct
    14101 gacataatca gtgcaggatc ttgccgctag tttacaactc gccttcgata gcggcgcaaa
    14161 acgagttctg ttgccgatat cctgggctat ggatattcca acagttccgg cagagttgtt
    14221 taccaagttt caggtaagtt tttactcaga accggttgat gcggtttata aggcgctgga
    14281 tgtgaattaa cacattcctc agcttattta caaaaggatt attcccattt gatataatat
    14341 cagtgccaat ttactttttt caatggaata tatctcggct aaataacaat aaaacttatc
    14401 gctaatggat taaataaaca attttctaat tcatt
//
LOCUS       DADLTD010000100        14412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLTD010000100 DADLTD010000000
VERSION     DADLTD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.100"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..421
                     /locus_tag="JN701_002395"
     CDS             <1..421
                     /locus_tag="JN701_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7364827.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            437..919
                     /locus_tag="JN701_002396"
     CDS             437..919
                     /locus_tag="JN701_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY7364828.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(996..1343)
                     /gene="rplS"
                     /locus_tag="JN701_002397"
     CDS             complement(996..1343)
                     /gene="rplS"
                     /locus_tag="JN701_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY7364829.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1385..2152)
                     /gene="trmD"
                     /locus_tag="JN701_002398"
     CDS             complement(1385..2152)
                     /gene="trmD"
                     /locus_tag="JN701_002398"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY7364830.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2183..2731)
                     /gene="rimM"
                     /locus_tag="JN701_002399"
     CDS             complement(2183..2731)
                     /gene="rimM"
                     /locus_tag="JN701_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY7364831.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2750..2998)
                     /gene="rpsP"
                     /locus_tag="JN701_002400"
     CDS             complement(2750..2998)
                     /gene="rpsP"
                     /locus_tag="JN701_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY7364832.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3135..4496)
                     /gene="ffh"
                     /locus_tag="JN701_002401"
     CDS             complement(3135..4496)
                     /gene="ffh"
                     /locus_tag="JN701_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY7364833.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4588..5454
                     /locus_tag="JN701_002402"
     CDS             4588..5454
                     /locus_tag="JN701_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY7364834.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            5475..6761
                     /locus_tag="JN701_002403"
     CDS             5475..6761
                     /locus_tag="JN701_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7364835.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6816..7409)
                     /gene="grpE"
                     /locus_tag="JN701_002404"
     CDS             complement(6816..7409)
                     /gene="grpE"
                     /locus_tag="JN701_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY7364836.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7532..8410
                     /gene="nadK"
                     /locus_tag="JN701_002405"
     CDS             7532..8410
                     /gene="nadK"
                     /locus_tag="JN701_002405"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY7364837.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8496..10157
                     /gene="recN"
                     /locus_tag="JN701_002406"
     CDS             8496..10157
                     /gene="recN"
                     /locus_tag="JN701_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY7364838.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10306..10647
                     /gene="bamE"
                     /locus_tag="JN701_002407"
     CDS             10306..10647
                     /gene="bamE"
                     /locus_tag="JN701_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY7364839.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10709..10999)
                     /locus_tag="JN701_002408"
     CDS             complement(10709..10999)
                     /locus_tag="JN701_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY7364840.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10989..11465)
                     /gene="ratA"
                     /locus_tag="JN701_002409"
     CDS             complement(10989..11465)
                     /gene="ratA"
                     /locus_tag="JN701_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY7364841.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11597..12079
                     /gene="smpB"
                     /locus_tag="JN701_002410"
     CDS             11597..12079
                     /gene="smpB"
                     /locus_tag="JN701_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY7364842.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12294..12656
                     /gene="ssrA"
                     /locus_tag="JN701_002411"
     tmRNA           12294..12656
                     /gene="ssrA"
                     /locus_tag="JN701_002411"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12817..14412)
                     /locus_tag="JN701_002412"
                     /pseudo
     CDS             complement(12817..14412)
                     /locus_tag="JN701_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         3558 a         3866 c         3394 g         3594 t
ORIGIN      
        1 gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg atggcgataa
       61 tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagggtct tgattgaaat
      121 cgcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgct tgggcggcga
      181 tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcaac agatatgctc
      241 agcactgaca caaatcttta atctcccgtt tgatcttcat aatggccatc agaccaccat
      301 gacattaagc attggttacg cgatgaccat tgagcacgcc tctgcggaaa aattacaaga
      361 gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc tggtgagata
      421 acaaggatat atcgatatga taaagcacct ggtagccccc ctgattttca cctcactaat
      481 actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg ccgctatgca
      541 atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag atgccattct
      601 gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc aaaccatggc
      661 agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca ccgataacta
      721 tggtgaagac agttacaacg aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc
      781 atgggctatt ggtggacaaa ttccacgcag caatctcacc acacagggtt taggaaaaaa
      841 atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt
      901 ggtgattaca accccttaaa acacgagcaa acagacaaaa aaagggccag ccaattggcc
      961 agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc tttgatacga
     1021 gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac agcaccacga
     1081 cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg ctcaacgcct
     1141 tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt acgaatagcg
     1201 ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac ccatactttc
     1261 acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg ctcttgttca
     1321 agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact gatatctcgg
     1381 gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg
     1441 ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca ggtcttctaa
     1501 gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca tggttgcccg
     1561 acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta tagtgcgggc
     1621 aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt cccagtaccc
     1681 ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca ccaccactga
     1741 gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg cgctcatcta
     1801 taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccaat tcgctgacgc
     1861 ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg ccttcacccg
     1921 ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt aacatccccg
     1981 gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag
     2041 gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt accccgtaat
     2101 cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac atagcgccgt
     2161 cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg attgaacgag
     2221 tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga cgttccttga
     2281 taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat ccggtttcca
     2341 tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg cagcccatca
     2401 ggtctttcca gtagtagtca ccctcttcaa gctgaggcaa ctgcgatgaa tccacgacaa
     2461 tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc agcttgatga
     2521 tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac tgacccgcct
     2581 tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca
     2641 ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg atgggatcaa
     2701 caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat taagctgctt
     2761 tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc tggccaaccc
     2821 agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta gcgattgggt
     2881 tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg tcagcgacaa
     2941 caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata gttaccataa
     3001 catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc ggtgtcatat
     3061 taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt aataactctg
     3121 tcgcataagg tcatttagcg accaggaaat cccggcggca tcatcccctt catgcttctc
     3181 atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
     3241 tgtttcagaa gacggttaac gtcctgcacc tgcataccgc aaccggcagc aatacggcgt
     3301 ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat cgagttgatg
     3361 atggcttcca tacgcaccag cactttatcg tccatctgtg acttgacgtt atccgggatc
     3421 tgccccatgc ccggcagctt gcccatcagg ctggccatgc cgcccatatt tttcatctgg
     3481 cgcagctgct caagaaagtc gttgagatcg aagccgtcac cttttttcag cttgctggct
     3541 aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
     3601 tcgcccatgc cgagaatacg cgacgcgatg cggtccggat ggaacggctc cagcgcctca
     3661 gttttctcgc caacaccgag gaacttgatc ggtttgccag tgatgtgacg aatagagagc
     3721 gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac
     3781 gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca
     3841 aacagggttt caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
     3901 tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg
     3961 gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc agaagggaag
     4021 aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc cgggcgataa
     4081 acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt
     4141 ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagcac gaccgcaggc
     4201 ggttgcgccg ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
     4261 cggacgattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
     4321 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac atccgcctcc
     4381 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt taggcgtcca
     4441 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
     4501 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
     4561 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
     4621 atactgcttc tctttcttat tgctcaaact gttgacatca ctatgcccgt ttttgctctg
     4681 ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
     4741 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
     4801 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
     4861 aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc
     4921 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
     4981 gcgaccttca tgcccaatga atacatcacc catctggaag caacgcctgg gatgctggtg
     5041 cacattggct tatcgctctt ttcctatgcc acgctaatta tcgccgccct gtacgcgctg
     5101 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcatttaa ccaggaaatg
     5161 ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg
     5221 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acctatttag catggaaaat
     5281 atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
     5341 cattaccatg aaggatggcg tggacgccgc gtcgtctggt ttaacgttgc gggcgcggta
     5401 attctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
     5461 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
     5521 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
     5581 atcgtctgcg acatatggcg aaacagggta atcgctcggc caaacgcgtc gaaaaattgc
     5641 tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg caataacctg gtcaatattc
     5701 ttgcctccgc gctcggcact attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa
     5761 ttgcgactgg tgtgctgact tttgtcgtac tggtatttgc tgaggtattg ccgaaaacca
     5821 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc
     5881 aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca
     5941 tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca
     6001 ctatcgtgca cgaatcacgc tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg
     6061 tgctcgatct ggaaaaaatg accgttgatg acatcatggt gccgcgcagt gaaattatcg
     6121 gtattgatat caacgatgac tggaaatcga ttctgcgcca actctcccac tcacctcacg
     6181 ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg
     6241 aagcctggcg attgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg
     6301 cggacgagat ctattttgtg ccggaaggta cgccgctcag cacgcagttg gtaaagtttc
     6361 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta tggagacatt caggggctgg
     6421 tgacggttga agatattctg gaagagattg tcggcgattt cactacgtcg atgtcgccaa
     6481 cacttgccga agaggtcacg ccgcaaaacg acggttcggt gattatcgat ggcaccgcca
     6541 acgtgcggga aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg
     6601 ttaatggcgt cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta
     6661 ttggcgagta cgatatagat attctcgacg tacaggacaa tatgattaag caggtaaaag
     6721 tttttcctgt gaaaccgctg cgcgagagtg tggcggagta acgaaaacgg ccctgcattc
     6781 gcatgcaggg ccgtgaatta ttacgaaaac agaaattacg cttttgcttt cgctacagtt
     6841 accatcgccg cacgaatcgt acgaccattc agcgtataac ccttctgcat aatgcccagt
     6901 acgttacctg gcgcaacgtc atcagattcc accattgcga tagcctgatg cacattcggg
     6961 tccagcggca cgttagtttc ggcgatcact tcaacaccaa acttacgcac aacatccagc
     7021 atcgacttca gcgtcagctc aatgccttca accatcgcag acatatccgg gttagcttta
     7081 tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc gttgatgaat
     7141 ttctccagcg caaatttgtg ggctttttca atatccagtt cagtacgtcg acgcaggttt
     7201 tccatttcgg cttttacacg caaaatgccg tcacgttcac gggtctgggc ttcagccagc
     7261 tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc agaagcttct
     7321 ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg ggcttgcccc
     7381 tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc gcattcatct
     7441 cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca ctcacattgt
     7501 catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt gtattggcat
     7561 tgtgggacac ccacggcacc ccactgcact gacaacacat gaaatgctct accgctggct
     7621 gtgcaaaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa
     7681 gaatgtgaaa actggcacgc tcgcggagat tgggcaacta gctgatctcg cggtagtcgt
     7741 tggtggcgac ggtaatatgc tgggcgcggc acgcacactc gcccgttacg atattaaagt
     7801 tattggaatc aaccgtggca acctgggttt cctgactgac cttgaccccg ataacgccca
     7861 gcaacagtta gccgatgtac tggaaggcca ctacatcagc gagaaacgtt ttttgctgga
     7921 agcgcaagtc tgtcagcaag attgccagaa acgcatcagc accgcgataa atgaagtggt
     7981 gcttcatcca ggcaaagtgg cacatatgat tgagttcgaa gtgtatatcg acgagatctt
     8041 tgcgttttct cagcgatctg atggactaat tatttcgacg ccaacaggct ccaccgccta
     8101 ttccctctct gcaggcggtc ctattctgac cccctctctg gatgcgatta ccctggtgcc
     8161 catgttcccg catacgttgt cagcacgacc actggtcata aacagcagca gcacgatccg
     8221 tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc agatagcact
     8281 gccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc tgaatctgat
     8341 tcatccgaaa gattacagtt atttcaacac attaagcacc aagctcggct ggtcaaaaaa
     8401 attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat actgtacgca
     8461 ataacagtaa tggtttttca tacaggaaaa cgactatgtt ggcacaactg accatcagca
     8521 actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc gtaataactg
     8581 gcgagaccgg cgcgggtaaa tctattgcaa tagatgccct cggtctttgt ctcggtggtc
     8641 gcgctgaagc cgacatggtg cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt
     8701 ctctgaaaga tacgccagcg gctctgcgct ggctggaaga aaatcagctt gaagacgggc
     8761 atgaatgttt gcttcgtcgc gtaatcagca gcgatggtcg ctcccgtggt ttcatcaacg
     8821 gtacagctgt tcctctgtca caactgcgcg aactgggtca gttgctgatt cagatccatg
     8881 gtcagcacgc tcatcaatta ctcaccaaac ctgagcacca aaaattcctg cttgatggct
     8941 atgccaatga aacctctcta ctgcaggaaa tgaccgcacg ttatcagttg tggcatcaaa
     9001 gctgccgtga cctcgcgcat catcaacagt taagtcagga acgcgccgcc cgtgcggaac
     9061 tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcccgga gagtttgaac
     9121 aaatcgacga agagtacaaa cgtctggcga acagcggtca attgctgacc accagccaga
     9181 atgcattggc attaatggcc tacggtgaag acgcaaacct gcaaagtcag ctttacacgg
     9241 ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc gtacttgata
     9301 tgctggaaga agctaccatc cagattgctg aagccagcga tgaactgcgc cactactgcg
     9361 atcgtctgga tctcgatccc aaccgactat ttgaacttga acagcgcatc tcaaaacaga
     9421 tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt gccacagtat taccagtcgc
     9481 tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg cttgcgctgg
     9541 cggtaacgaa acatcatcag caggcactgg aaatcgcgcg cgcattacac caacaacgcc
     9601 agcaatatgc agaagaactt gcacagctga tcaccgacag tatgcatgcg ctctcaatgc
     9661 cgcatgggca gtttacgatc gatgttaaat ttgacgagca tcacctgggc gctgacggtg
     9721 ccgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag cctattgcca
     9781 aagtcgcatc cggtggtgaa ttgtcccgca tcgcactggc aacccaggtc atcacggcgc
     9841 gtaaaatgga aaccccggca ctgatttttg atgaagtgga tgtagggatt agcggtccaa
     9901 cagcggcagt tgtcggcaaa ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg
     9961 ttacccacct gccacaagtc gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa
    10021 ccgatggtgc gatgacagaa acgcatatgc aatccctgaa taaaaaagcg cggttacaag
    10081 agctggcgcg cctgcttggt ggcagtgaag tcacacgtaa tacactggcg aatgcgaaag
    10141 aactgcttgc agcgtaaact tttttcctgc ttcacggtca gagtaaacag caaaacgccg
    10201 taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc ggcatattac
    10261 agatgagcca cgtactgcta gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg
    10321 ctgactgctg cagcagcagt actattgatg ttgaccgcag gctgttccac tctggagcga
    10381 gtggtttacc gtcctgacat caaccagggg aactatctga ccgctaacga cgtatccaaa
    10441 atacgtgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc
    10501 gatccatttg gtacgaatac ctggttctat gtcttccgcc agcaaccagg tcatgaaggt
    10561 gtaacgcagc aaacgctgac gctgaccttt aacagtagcg gtgtgttgac caatattgat
    10621 aacaaacctg cgctgagtgg taactaataa taaagttgct ctcaaagacg ttaaaaaagg
    10681 tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct gctcgttgcc
    10741 tgcgaagctc tttcggatcg gcaatgagag ggcgataaat ctccacccga tcgccatcat
    10801 gcacactatc gcttagtttt gccggacggc tgtaaatgcc gactttgttt ttagttaaat
    10861 cgatatcggt acgcaattcc agcaagccac tggcgcgaat agcttcttca accgtcgcgc
    10921 cctcctgcag cgtcacgcgc tgcaggtact gcttctcagg tagcgcataa gccacctcaa
    10981 cggcaatttt acctggcact gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata
    11041 ttagacgcca gctctttaaa cacgcgacca aaggcgagtt caatcaactt attggtaaac
    11101 tcaaagtcga gatgaaactc gatacgacac gcctcctggc tcagcggcgt aaacttccat
    11161 ccaccaatca atttcttgaa cggcccatcc accagattca taagaatact ttggttactg
    11221 gtcaactggt tgcgggtagt aaacgttttg ctgatcccag ccttagagac atctaccgcc
    11281 gcagtcatct gcccaggagt ggactccaga atccgacttc cggtacaacc cggcaaaaac
    11341 tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct gtagggtacc
    11401 agtgcggtcc ggctaatctg aggcataact atttccatca acaaaaatcc aacaaataat
    11461 atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc gggttcatgc
    11521 taagatagag ccttgtcccc cgcaggattg atatggggtg ttttcgattt cagattaccg
    11581 atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag cgaccatcgc
    11641 gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag cgggacttgc
    11701 cctgcaaggc tgggaagtta aatccctgcg cgcaggaaaa gccaatatca gcgacagcta
    11761 cgtccttctg cgtgacggag aggcatttct gtttggcgct aacatcacgc caatggccgt
    11821 ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc tcaaccagcg
    11881 cgaactggac tcattgtacg gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc
    11941 cctatactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca
    12001 gcacgataaa cgttcagata tcaaagaacg cgaatggcag gtggataaag cacgtatcat
    12061 gaaaaacgcc caccgttaaa cctgcactcc aattattgac cagttcctca ccgcgcctcc
    12121 ctctccggcg gcgcgaatga acatcttatt ggctatcaca tccgacacaa atgttgccat
    12181 cccattgctt aatcgaataa aaatcaggct acatgggtgc taaatcttta acgataacgc
    12241 cattgaggct ggtcatggcg ctcataaatc tggtatactt gccgttacac attggggctg
    12301 attctggatt cgacgggatt tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg
    12361 taaaaagccg caaaaaatag tcgcaaacga cgaaaactac gctttagcag cttaataacc
    12421 tgcttagagc cctctctccc tagcctccgc tcttaggacg gggatcaaga gaggtcaaac
    12481 ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt aatcaggcta
    12541 gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg actaagcatg
    12601 tagtaccgag gacgtaggaa tttcggacgc gggttcaact cccgccagct ccaccaatca
    12661 tgattggacg gtataaggac aacaccaata aaaacaggag gttagcagtc tcagcaggac
    12721 accgaccaga cggtgaagag acataaaagg atacgcaaag gagccgcggc tcctggtgac
    12781 atgaaagccc acagatgtgg gctttttcgt tgatggtcag aacgaccagt tcacaccagc
    12841 taccccgttc cacggggatt ccacaccggc accatggcta taccccaccc caagatgccc
    12901 gcttaacgta ctgctgaatg aggctttaat acctgcctgg tatattccac gtctgcccga
    12961 caaatcattg acgaaattac cgtcactatt cactttcacc cggttatcat cgacaaattc
    13021 tttgcgcaca gccgccttca gccacggctc aacttccata ccgttcccca gacgcatgtt
    13081 gtaactcagc gttgcaccca gttcacgata taaacctgcg ggtatcgact gatttcgatt
    13141 ccatgccatt ggataaatga tattcagggt tatcagcggt gaacccccgt taacgatgca
    13201 tacggcgtca ggttccagtt accatcggta aatcgcatcc cggtttcaat gtgaccgccc
    13261 agcccgttgc tgcgataact gccgttggtg gctccaccgc tgccaaccgt ggagtcgcga
    13321 gcttcggtac ctgctaatac caataaatta ccgccatttt ccagcaacat attggtcgct
    13381 aaattgccgg aaatggaaaa agtgccgtac tggtgagtac cgctgatttc aataccgtta
    13441 gccgtgctcg tctggagagc ggcaccgctg ttctggacga tatctgtcgc tttgccatta
    13501 tcgttaactg tcagcgtacc gccttcattg atctttgttt ttattgcctc tccgttagct
    13561 gaaactgttt gtattccgcc gtcgttaatc gttgtctcat tcgccacccc ctcgacgatt
    13621 tgttcgccgc cggtgagcgt cgtgcctgtc gcagtggctt ttgttttgac gatctcccgt
    13681 ccgcccgtat tgacctgtgt tttgtcagaa gaggtgtctg actccacggt taacacgccg
    13741 ccggattgtt gctcaacatg cttagcctca ccaccgtcct gaacctccag aacgccacca
    13801 ttattaagtc tggtggtatc tgttttagcc tccttctgga caatcagctt accgccagca
    13861 tcaacggtag tatttttcgc cgaggtttta gccactaccg tcagttcgcc ggtattttcc
    13921 agcacaacat aattagcctc ccctccgggt aatagtgaag tgagagagct tgttgtatcc
    13981 ttcgatatca gtccctgcgc ccgtgttggc aactaaagca ctgcccgtct cctggttaac
    14041 cccatgtgct ataccgccgg tatagacaat cagcgagcct ccggcgctaa tattgctgcc
    14101 agttgccgta ccatctttct caacaacctg ccggctcccg gtggaaatat ttgtcgtgtc
    14161 agctttcccg ccgctcttga tattttgtgt tccgctgttg atattggtac ccgtggcaat
    14221 accatgatta ttaatattct gtgtaccacc attgattatg gtattggtcg cgtttccggt
    14281 aacatccatt accccgccgt tatctattcg ggtcgcatca gctttagcat tcgttaaaac
    14341 tgacattgtt cctttatctt taataatcgt cttatttgcc gaaccatatg cgtttatgtc
    14401 taaatgacca cc
//
LOCUS       DADLTD010000101        14379 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLTD010000101 DADLTD010000000
VERSION     DADLTD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.101"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..322
                     /locus_tag="JN701_002413"
     CDS             <1..322
                     /locus_tag="JN701_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7364843.1"
                     /translation="EADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVR
                     WGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAASADAIASLLQH
                     ELEL"
     gene            334..996
                     /gene="fsa"
                     /locus_tag="JN701_002414"
     CDS             334..996
                     /gene="fsa"
                     /locus_tag="JN701_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7364844.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            1007..2110
                     /gene="gldA"
                     /locus_tag="JN701_002415"
     CDS             1007..2110
                     /gene="gldA"
                     /locus_tag="JN701_002415"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HAY7364845.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            2384..3001
                     /locus_tag="JN701_002416"
     CDS             2384..3001
                     /locus_tag="JN701_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7364846.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(3028..3966)
                     /gene="yijE"
                     /locus_tag="JN701_002417"
     CDS             complement(3028..3966)
                     /gene="yijE"
                     /locus_tag="JN701_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY7364847.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(4027..6207)
                     /gene="katG"
                     /locus_tag="JN701_002418"
     CDS             complement(4027..6207)
                     /gene="katG"
                     /locus_tag="JN701_002418"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY7364848.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(6536..7426)
                     /gene="metF"
                     /locus_tag="JN701_002419"
     CDS             complement(6536..7426)
                     /gene="metF"
                     /locus_tag="JN701_002419"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY7364849.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(7775..10207)
                     /locus_tag="JN701_002420"
     CDS             complement(7775..10207)
                     /locus_tag="JN701_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY7364850.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(10210..11370)
                     /gene="metB"
                     /locus_tag="JN701_002421"
     CDS             complement(10210..11370)
                     /gene="metB"
                     /locus_tag="JN701_002421"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY7364851.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            11647..11964
                     /gene="metJ"
                     /locus_tag="JN701_002422"
     CDS             11647..11964
                     /gene="metJ"
                     /locus_tag="JN701_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY7364852.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            12148..12756
                     /locus_tag="JN701_002423"
     CDS             12148..12756
                     /locus_tag="JN701_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY7364853.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            12901..13576
                     /locus_tag="JN701_002424"
                     /pseudo
     CDS             12901..13576
                     /locus_tag="JN701_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(13748..14020)
                     /locus_tag="JN701_002425"
     CDS             complement(13748..14020)
                     /locus_tag="JN701_002425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364854.1"
                     /translation="MELQLSDKGVIKMKAIQCCYFLALVSSNLMAGVMGNDNIFLSNS
                     DSAALYCVAKIFPDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(13986..14379)
                     /locus_tag="JN701_002426"
                     /pseudo
     CDS             complement(13986..14379)
                     /locus_tag="JN701_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
BASE COUNT         3549 a         3896 c         3587 g         3347 t
ORIGIN      
        1 ggaagccgac ttatgggcgc gcgaggccgt tttctctacc ggtctgggct ttagttttgc
       61 cattccacac agcaaatcag aacacattga gcaatccacc atcagcgtgg cgcgtctgca
      121 agcgccggtg cgctggggcg atgatgaagc gcaattcatc attatgttaa ccctgaacaa
      181 acacgctgcg ggcgatcagc acatgcgcat tttctctcgc ctcgctcgcc gcatcatgca
      241 cgaagaattc cgtaacgcgc tggttaacgc cgcctctgcc gacgctatcg ccagcctgct
      301 gcaacatgaa ctggaactgt aaaaggaaac atcatggaac tgtatctgga caccgctaac
      361 gtcgcagaag tcgaacgtct ggcacgcata ttccccattg ccggggtgac aactaacccg
      421 agcattatcg ctgccagcaa ggagtccatc tgggaagttc tgccgcgtct gcaaaaagcg
      481 attggtgatg agggcattct gtttgctcag actatgagtc gtgacgcgca gggtatggtg
      541 gaagaagcga agcgcctgcg cgacgccatt ccgggcattg tggtgaaaat cccggtgact
      601 tccgaaggtc tggccgccat taagatgctg aaaaaagagg gtattactac gcttggcacc
      661 gcagtgtaca gtgctgcaca agggttactg gccgcgctgg caggggcaaa atacgttgct
      721 ccgtatgtta accgcgtaga tgcccagggc ggggacggca ttcgtacggt tcaggagctg
      781 caagcgctgt tagaaatgca cgcaccagaa agcatggtgc tggcggccag ctttaaaacg
      841 ccgcgtcagg cgctggactg tttactggca ggatgtgaat ccatcaccct gcccttagat
      901 gtagcgcaac aaatgctcaa cacccctgcg gtagagtcag ctatagagaa gttcgaacac
      961 gactggaatg ccgcatttgg cactactcat ctctaaagga gcaattatgg accgcattat
     1021 tcaatcaccg ggtaaataca tccagggcgc tgatgtgatt aatcgtctgg gcgaatacct
     1081 gaagccgctg gcagaacgct ggttagtggt gggtgacaaa tttgttttag gttttgctca
     1141 atccactgtc gagaaaagct ttaaagatgc tggactggta gtagaaattg cgccgtttgg
     1201 cggtgaatgt tcgcaaaatg agatcgatcg tctgcgtggc atcgcggaga ctgcgcagtg
     1261 tggcgcaatt ctcggtatcg gtggcggaaa aaccctcgat actgccaaag cactggcaca
     1321 tttcatgggt gttccggtag cgatcgcacc gactatcgcc tctaccgatg caccgtgcag
     1381 cgcattgtct gttatctaca ccgatgaggg tgagtttgac cgctatctac tgttgccaaa
     1441 taacccgaat atggtcattg tcgacaccaa aatcgtcgct ggcgctcctg cacgtctgtt
     1501 agcggcaggt atcggcgatg cgctggcaac ctggtttgaa gcacgcgcct gttcccgtag
     1561 cggcgctacc actatggcgg gcggcaagtg cacccaggcg gcgctggcac tggcagaact
     1621 gtgctacaac actctgctgg aagaaggcga aaaagcgatg cttgccgccg aacagcatgt
     1681 agtaacccct gcgctggaac gcgtgattga agcgaacacc tatttaagcg gtgttggttt
     1741 tgaaagcggc ggactggcgg cggctcatgc ggtgcataac ggcctgaccg ctatcccgga
     1801 cgcgcatcac tattatcacg gtgaaaaagt ggcattcggt acgctgacgc agctggtact
     1861 ggaaaacgcg ccggttgagg aaatcgaaac cgtagctgca tttagccatg cggtaggttt
     1921 gccaataact ctcgctcaac tggatattaa agaagatgtc ccggcgaaaa tgcgaattgt
     1981 ggcagaagcg gcatgtgcag aaggtgaaac catccacaac atgcctggcg gcgcgacgcc
     2041 agatcaggtt tacgccactc tgctggtagc cgaccagtac ggtcagcgtt tcctgcaaga
     2101 gtgggaataa cctactccaa actcccggct tgtccgggag tttgaacgca aaattgcctg
     2161 atgcgctacg cttatcaggc ctacgcaatc tctgcaatat attgaatttg cgtgcttttt
     2221 aggccagata aggcgttcac gccgcatccg gcactctgtg ccagtaatct gaaactccta
     2281 cgttgttcag gagtttttta tccgcttctg gacattttct ctacagaaca atcgttagcc
     2341 cggaaggtcg aatctgtgac tcttattttc acggacgaac cagatgaaag cttcactggc
     2401 gctgctcagc ctgctaacgg catttaccag ccactcctta aaatctcccg ccgttccacc
     2461 aaccgtggta caaatccagg ccaataccaa ccttgccatc gcagacggtg ccagacagca
     2521 aatcggcaac acgctatttt acgatccggc gtatgtgcgg cttacctatc ctggcggtga
     2581 tgtcccgcaa gaacgcggtg tatgttccga tgtggtgatc cgtgcattgc gcagccagaa
     2641 ggtcgatttg cagaaactgg ttcacgaaga tatggcgaag aattttgccg agtacccgca
     2701 aaaatggaag ttaaagcgcc cggacagcaa catcgatcac cgtcgggtgc ctaatctgga
     2761 aacctggttt agccgccaca acaaaacacg ccccatcagt aaaaatgccg gtgactatca
     2821 agcgggcgat attgtctcct ggcgattaga taacgggctg gcgcatattg gggtggtgtc
     2881 agatggcttc gcccgcgacg gtacgccact ggtgatacat aacattggcg caggcgcgca
     2941 ggaagaagat gtgctgttta gctggcggat ggtggggcat tatcgttatt ttgttaaata
     3001 agcgatcgcc ccacatgaag aaaaaattca gatgcttttt acactgacag cttctttttt
     3061 cttacggctc accagcgcca gtgccagcac aatcagcaca ataccgctac cttcaacggc
     3121 ccccggattc tcaccgagca gccaccagga aaagagtacg ccgcaaacgg gaacggccag
     3181 cgtgcttaag ctggcaatac tggcaggcaa gtttttcaat acaaacaacc ataagctcca
     3241 cgccagcgcc gtcgccagaa tcgcactgta ggccagcgcc cagaacacgg tgggctgcca
     3301 gtcaatttca cgttgcggca ccagtaaagc gaccacactc atcaccagcg ccgcatagag
     3361 catctgccag gatgttaacg ataataaatc cacgcgcgga tgacgggtgt ataggcgttt
     3421 agcaacaatc gcgctcgccc cccaactaac accggagagg attgccagca tggcactttt
     3481 catcgaagag aaatcgagtt gccacggctg caacaccaaa aataagccga aagcggcaat
     3541 cagaatcgcg aaatattgcc cacgtcgcag gcgttcaccg agaaacaacg cggcgaaaat
     3601 caccacccag aacggcatgg tatagctcag gatcgccacc ttccccgcac ctccgctgac
     3661 caacgcccac tgcgccagac caaccatccc gcaggtttgt aacagggcaa tggctaaggt
     3721 gtatttaaac ggcgtcgggt gcattccgcg accacgtaat aaaaggacga tgaataaaac
     3781 gagagcgccg aaaatgcagc gtaaggcggt aaagtcgaag gcaccgatgt aactggtgac
     3841 ttgcttcatg aaaatccagc tataactcca gataagtgtg agcacaacca ggccactgat
     3901 tgccagtggg ttgctctttc ctgcggcaga catgagagat ccagtgtgta gttttcctgt
     3961 ggacactata cgccggtaaa gaaacttcag cgactgcctg aaagacagtc actgaagaag
     4021 gtcagattac agcaggtcga aacggtcgag gttcatcact ttcacccatg ccgctacgaa
     4081 gtctttaaca aacttctcgt gggcatcgct gctggcgtaa acttccgcta ccgcacgcag
     4141 gacagagtta gaaccaaata ccagatccgc acggctggct gtgtatttca cttcgccggt
     4201 ttcgcggtca cgtccttcga acagctcttt cgattcgtca gtcgctttcc actcgtaacg
     4261 catatccagc aagttcacga agaagtcatt gctcaatacg ccaacgcggt cagtgaagac
     4321 gccgtttttg ctgctatcga agttggcacc cagtacacgc atcccgccca ccagcgcagt
     4381 catttccggc gcggtcagcg tcagttgctg tgctttgtcg atcagcagtg actcggtggt
     4441 ggaaacgtcc agacgagcgc gatagttacg gaaaccgtca gcaattggct ccagcagctc
     4501 aaacatctca atgtcagtct gatcctgacg cgcatcaacg cgacccggcg caaacggtac
     4561 atgaatgctc aaacctgcgg cgcttgcggc tttctcaaca ccaaccacac cagccagcac
     4621 gatgatatcc gccagcgagg ctttaccaga ctctttctgg attttctcca gaacaggcag
     4681 agcacgaacg gctgcggcgt tcacatccca gtcgcgctgc ggcattaatg ccagacgcgc
     4741 accgttggca ccaccgcgtt tgtcgccacc acggaaggta gaagcagatg cccaggccac
     4801 cgataccagc tcactaacag acagaccaga atccgcaatc gcgaatttca gatcgataat
     4861 gtcctgctcg gtcgggttgt agatcggctg cggcagcgga tcttgccaga tcagatcttc
     4921 tttcggcact tccggcccga tgtagcgaga tttcggcccc atatccctgt gcgtcagttt
     4981 gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga tcgttgagga aacgacgaga
     5041 gatcttctcg aactcaggat caaaacgcag cgtcaggtcg gtcaccagca ttgtcggttt
     5101 acgtttcttc gacggatcga acggatccgg gataatttcc ggtgcgtcta ccgcttcgaa
     5161 ctggattgct ccagccggac tgcgggtctg tacccactca tatttgaaca ggttctcgaa
     5221 gaaatagttg ctccactggg tcggcgtctg ggtccagaca acttccagac cagaggtaat
     5281 ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccag cctaaacctt gttcttcaat
     5341 cggtgcagct tctggatcag gacctacatt tgatgtcgga ccggcaccgt gagttttacc
     5401 cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg tcgttcatgc ccatgttgcc
     5461 gaaggtcgca cggatagctg ctgccgcaga aagcggttcg ccgctgtgat ccgggccttc
     5521 cgggttaacg taaatcagac ccatctcggt tgcacccagc ggtgctttcg ccagcgcttc
     5581 cggatgacgg tgagtcagcc aggctttttc atcaccccag ttaacatcca gatccggttc
     5641 ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg aagccggagt tttccagcgc
     5701 cacgttacct gcgaggataa acaggtcggc ccaggagatt ttctgaccat atttctgttt
     5761 gattggccac aacaggcgac gcgctttatc aaggcttacg ttatccggcc aggagttcag
     5821 cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgt ccatcgattg aacggtaagt
     5881 acctgcgccg tgccaggcca tacgaataaa cagaccggcg tagctgcccc agtcggctgg
     5941 ccaccacggc tgagattctg tcagcagggc tttcagatct tttttcaggc cgtagtaatc
     6001 taatttgctg aattctttgc ggtagtcaaa gtcctcaccc agcgggttag aacgattaga
     6061 atgttggttt aacaggtcaa cacggagttg atttggccac cagtcgcgag tggttgtgcc
     6121 cgcccccgcg ctctggtcgt gaccgccctg atggaacggg catttgccag tggctgtcgt
     6181 gttatggata tcgtctgacg tgctcatcaa tgtgctcccc tctacagtgt taccgttacg
     6241 atacacagcg ttagagagaa gttataattg aattaatcca cggatgcgat agttgagatc
     6301 ttacatattg ttggttaaag agatgtaggt caaattgatc gtaattaagc ttttttgatt
     6361 atgtccgaat tcggacaaat gctttataaa aaagggtttt agcttcatat ccttcaagta
     6421 atgagggaga aaacaaggct gacaacctta ttttcccgcc ctcatttcga ggcagcattt
     6481 tgtgctctgt ttaaaatttg tgatcactgt gtgattttca caaaagccac actatttata
     6541 aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact catttcagcg cggttaagcg
     6601 tatagaagtg gaaatctttc actccttcac ggcttaaaat cttcaccata tccatggcaa
     6661 tattcgcgcc caccagtttg cgggtttcgg catcatcatc caggccgtcg aacatttgcg
     6721 ccatccacgc cggaatacgc acgttggtca tatcggcaaa tttcttcgcc tgtttaaagt
     6781 tagataccgg caaaattccc ggaataattt ccacatcaat gcccgccgat acacagcggt
     6841 cacgaaaacg caggtagctt tcgacatcga agaagaactg agtaatcgcg cggttggctc
     6901 cggcatcgac tttgcgtttc agattaagca aatccgcctg agcgcttttt gcttccgggt
     6961 gaacttccgg atacgccgcc acggagatat cgaaatctgc cacttctttt aatagcgtca
     7021 ctaggtcaga agcatacatt tctggcttac cacttcccgg cggcagatcg ccacgcagcg
     7081 ccacgatatg acgaataccg ttattccagt agtcgcgtgc aatggtgcgc aactcgtcgg
     7141 gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag accagtgcga tctttaatgc
     7201 ctttaataat gctgtgcgta cggtcgcgct cgccggagtt cgcgccatag gtcaccgata
     7261 caaacttcgg tttcaggctg ctaaggcgat cgatggagtt ccacagggtc tgctccattt
     7321 cactggtacg cggcgggaaa aactcgaacg aaacgttaat ctgcccctgg acttctgcca
     7381 ggcgctgatt cagggcatcc cgctggctgg cgtgaaaaaa gctcatacct tacctcatca
     7441 atcgcttgta gttatatgtt gtgttgtgca catctatccg tttagacgtc cagatgtaaa
     7501 gatgaaggaa aagccgaagg gcgtcaaccg aaaaatgacc aatgatggtg aggaaaaatc
     7561 agggaagatg aaaaaacttc aggatggtaa gaaaaagaaa atccggtatg caaagcaaac
     7621 cggactgcaa atataaacta accatgtact tattgcttct tgccggattc gtcgtaaacg
     7681 cttaatccgg cctaatccta tgataattca atatatttca ttagaaacca taaacccgaa
     7741 aacatgagta ccgggcatta ttaaatttct gaaattacaa caactgtgcc agccggttga
     7801 tatccgactg aatcgccccg gcggtgacgt cgcgccccgc gccaggccca cggatcacca
     7861 gcgggttgtc gcgataccag cggctttcga tggcaaagac gttatcgcac ggcagcagtg
     7921 atgccagcgg atggtcttca cgcaccgcct ccacgcccac acgcgctttg ccgttggcat
     7981 cgaaacgcgc cacgtagcgc agcaccagcc ccatttcgcg ggccgcttcc agccgttgca
     8041 ccatctgctc gttaagttca tcgccatttt caaagaaatg gtcgatgctg ccgccttcgc
     8101 aatgagcagg caccagcgat tccacacgta cctggtccgg ttcaatgttg taacccgctt
     8161 cacgcgccag aattaccagc ttgcgcatta cgtctttgcc ggagaggtcg tcacgcggat
     8221 caggctcggt taagccctgc tgccacgcct gatccaccag ctcggtaaac ggcacgctac
     8281 cgtcgaattg caggaacagc caggagagcg tgccggagaa gatcccgctg atcgacaaaa
     8341 tagtatcgcc gctgtcgatc agatcgcgca cggtgtggtt gatcggcaag cccgcaccga
     8401 cggtggcatt gtacagccag tgacgcccgg ttttttcgaa ggcgtcgtgg atctggcgat
     8461 atttattgct gtcgctggct cccgccagtt tgttggcgct gataacgtgg aaaccgtggc
     8521 tggcgaaatc aagatactga tcagcaagct gctggctggc ggtaacgtcc agcaccacta
     8581 aatcatcata cggatgggcg cgcatccaca ggaacaacga ctcttcatcc tgctcaaccg
     8641 cttcatcgtt gaagaaggct aacgcgcggc tggcgtccag cccgtcatag ctcaacaggc
     8701 tgcggcggct gtccaccaca cctgccagca caaactcaaa gccggtacgt gccgaaagcg
     8761 tgctctgctc acgggcgaac agttccagcc aacgggaacc gatattgccc ttaccgaaca
     8821 ataccaggcc gatgcgtttt tctgcgcgga agacggactg atgcagcccc tgaatcaggc
     8881 tttcggtcgg gccggtgcgc agtactgcca ccaggctgat gccgtcatcg gactgccagg
     8941 taaattcgac cggctggcct ttcagttgct gccagaagcg gtggcaatgc agcgggttac
     9001 gggtgacgcc tgcaccgacc atcgccacca gcgccagccc ctgacgcagg cgcagttcgc
     9061 caggtaatcc cgcttcgtcg aggattttca gcgcactgtc ggccacttct gaggtgtagc
     9121 aaaattgcag caactggcga tcgttatgta cgccaaccgc cagcgggcgt acctgcgcgc
     9181 gtttcaggat ttggtcgatc tctttatgcg ccagtttgaa atcctgactg gcgggcacct
     9241 gaaactcaat caaacagaca tcatcgtggc tggtgacaat acgcgcacca gtaccggagg
     9301 ccagcacgcg ttcaatgcgc gtggaacctt gatccggcgt gtagctacag cgcagttgca
     9361 ggtcgatttc gctgccagaa accggctgta aagtacgggc gtgaagaacg ggagccgcca
     9421 ggcgcgccag ttcgctggcc tcatccagac gcagcaacgg cagcaggcag gcatctttca
     9481 ctttacgcgg gtcggcactg tataccccgg cgacgtcgct ccagatggtt acgcgagaaa
     9541 cacccgccag cgcaccgatt tgtgtcgcgg aatagtcgga accgttacgc cccagcagca
     9601 ccgtttcacc ggcgttgttg cggctgataa atccggtcac caccagacgt ttgcccggat
     9661 gttgcaccag cagctgttgc agcaacgggt aagaaagccc ttcatcaacc tgcggttgtg
     9721 cggcgcgttc agcgcgtaaa aactcgcggg catcaagcca ggccgctggc agcccttgtt
     9781 gattaagtac cgcagacatc aggcgtgccg accatacttc cccgtggccc accacttccg
     9841 catacactgc gtcgttaata ccgctgtcga gcagcgccgc caggcgctca aggtcgctga
     9901 caaaagcgct aatgaggcta tcagcttctt cagcgggtag cagaccgcta atcagatcgc
     9961 actgatagcg acgcagcgtt tgttgaacct gatgcgcaga gagacgatcg gtctggctta
    10021 gtttcaacca gttaatcaac tggttagtgg tgctaccggc ggcggaaacc accatcatat
    10081 cgtcaggctg agagtactcc gccataatgc ccgcaacacg caaataacac ttcacatcag
    10141 ccagactact gccaccaaat ttatgcagtt gacgaccttt cgcccctgcc tgcgcaatca
    10201 cactcatttt taccccttgt ttgcagcccg gaagccattt tccaggtcgg caattaaatc
    10261 ttcgccatct tcaataccgg tggagatacg cagcagcgtc tcggagatcc cggcggcagc
    10321 acgcgcttct ggtgccatgc ctgcatgtgt catggttgcg gcgtgagaga ttaaactttc
    10381 cactccccct aatgattccg ccagcgtaaa caacgacagc ccgcccagga aacgacgcag
    10441 cgtctgctca tcgccatcca gttcaaaact caacattgcg ccaaagcctt tttgctggcg
    10501 cgcggcaatt tcatgcccct gattttccgg cagcgacggg tgatacagtt ttttcaccaa
    10561 cggctgggtt tgcagatatt tcacaatcgc ctgcgcgttg cgctgggcca gctccatacg
    10621 cggcaccagc gttcgcaacc cgcgtagcag cagatagctg tcaaacgcgc cgcccgtcac
    10681 gccaatattg tttgtccacc aggcaagttc agtgacaacg tccgggtctt tagcaatcac
    10741 cacgccagcc accacgtcag agtgaccgtt cagatatttc gtacatgaat gcaacaccag
    10801 atcggcaccc aatgccagcg gattttgtaa tgccgggctt aagaaggtgt tatccaccac
    10861 gctcaccgcc ccgacttccc ttgccagatg gcagattttc gcaatatcca cgacgcgtaa
    10921 caatggatta cttgggcttt ctaccagtac cagtttaggt ttttctgcca gcgctgcccg
    10981 taatgcctgt tcatcgcctt gatcaacaaa caacacgcga tagcaaccgc gtttcgccag
    11041 actgtcgaac aggcgatagc taccgccgta gcagtcgtgc ggcgcaacca gcagatcgcc
    11101 aggtttcaaa aagacggtcg ttaccaagtg aatcgcggac atgccggtat tagtaagtac
    11161 tgcgccagca ccgccttcca gttctgccag cgcacgctga accacatcgc gcgttgggtt
    11221 gccgcgacgc gagtaatcat gcgcgcgcgg ttcattaaat ccggtaaagt tataggtgct
    11281 ggaaagatgg atgggaggga caacgcaacc atactgttcg tcgtcattta acccgctacg
    11341 cactgcgatg gtggcctgtt tacgcgtcat gtgatgaagt ttcctgggct ttgtcggtga
    11401 aatgtcaggc accagagtaa acattgtgtt aatggacgtc aatacatctg gacatctaaa
    11461 cttctttacg tatagattga gcaaatccca aatagccgtt aaaattatat gcattatcac
    11521 gccgacaggt gcattacacg atgtcacggt aacgcctgta cggtaaacta tgcgggttta
    11581 cggtcagtac ccacataaat tgtgtggtct ggtctcaatt tattgacgaa gaggattaag
    11641 tatctcatgg ctgaatggag cggcgaatat atcagcccat acgctgagca cggcaagaag
    11701 agtgaacaag tcaaaaagat tacggtttcc attcctctta aggtgttaaa aatcctcacc
    11761 gatgaacgca cgcgtcgtca ggtgaacaac ctgcgtcacg ctaccaacag cgagctgctg
    11821 tgcgaagcgt ttctgcatgc ctttaccggg caacctttgc cggatgatgc cgatctgcgt
    11881 aaagagcgca gcgacgaaat cccggaagcg gcaaaagaga tcatgcgtga gatggggatt
    11941 aacccggaga cgtgggaata ctaaagcaaa aaagagcggc gcggagtgga atcgcctgat
    12001 gcgctacgct tatcaggcct acgtcatatt gcaatttatt gaatttgcac gaacttgtag
    12061 gccggataag gcgttcacgc cgcatccggc ataaacaacg agcacgttgt ctgcgaccca
    12121 ccgcttttta tacatggacg tttaactatg aaaaacaggc tgctgatcct cagcctgctg
    12181 gtttctgtac ctgcctttgc ctggcagcca caaaccggcg acatcatctt tcagatctct
    12241 cgctcatcgc aaagtaaagc gatccaactg gcgacccatt ccgattatag ccacaccggt
    12301 atgctggtga tacgcaacaa aaagccctac gtttttgaag cggtcggccc ggtgaaatac
    12361 accccgctca agcagtggat cgcccatggt gaaaagggca aatacgttgt tcgccgcgtt
    12421 gaaggcggac tgagtgttga acaacagcaa aaactggcgc aaacggtaaa acgttatctc
    12481 ggtaaaccgt atgatttcag cttctcatgg agcgacgatc gccagtactg ttcggaagtg
    12541 gtgtggaagg tttaccagaa cgcgctggga atgcgcgtgg gcgagcagca gaagttgaaa
    12601 gagttcgatc tcagcaaccc gctggtccag gctaagctca aagaacgcta cggcaaaaat
    12661 attccgcttg aggaaacggt agtttcacca caagccgttt tcgacgcgcc acaactcacc
    12721 acggttgcca aagagtggcc gctgttttcg tggtaatgga tgatgaaaaa aataattccc
    12781 gccgtagtct gtttgttgct ttgtagttgc acgacctttc cgcaaactgt cgctccggta
    12841 aacggtgatg cctccaatta gttgaattaa ggatataata ctcgctttta aggttgtttc
    12901 atggtcacag ttaaccttca ttgcccccgt tgccagtccg ttcaggttta ccgtcacggg
    12961 cagaacccaa aaagacatga tcgattccgc ggccgtgact gtcaccgggt atttcagctc
    13021 agttactgtt acgagccccg taaaccggga gttaaagacc aaatcgctga aatgactttc
    13081 aacggtgccg gggttcgcga tactgcccgt acgctgaaaa tcggcattaa tacagtcatc
    13141 cgaactttaa aaaactctcg ccacgaagaa taacgtcatc cccggtcgct catgctgatg
    13201 tggcactcat ctgcgaactt agtcagcaat ggagctttgt cggcagtaaa gcgcgacagc
    13261 actggctctg gtacgtgtac aacacgaaaa ctggtggtgt tctggcctac acttttggcc
    13321 cccggactga cgaaacctgt cgtgaattgc tggcgctgct taccctttta ccatcggcat
    13381 gttgaccact gacgaatggg gtagttacac cagagagtta ccgaaggaaa agcatctgac
    13441 tggtaagatt tttactcagc gcattgagcg caacaatctg accctgagaa cccgaatcaa
    13501 acgtctggcc cgcagaacca tctgtttctc gcgctctgta gaacttcatg aaaaagtcat
    13561 cggtgctttt atcgaaaata tatgttctac taattggagt catcaccctt ctgatgagct
    13621 ttccttggat gattaccaga ctgaatacac agccaaagat ttttacctca ttctgctctt
    13681 ttttctcaaa tgacatatat tatattttac atcatattca taggcatatg acattccccc
    13741 gcccccatta tgtcattgtg ggttgatgtt gggctttcat aaaaaaaaca tctttttttg
    13801 aactagcatt aattatattt actttatata ttaaaatatt ttctccatct ggaaatattt
    13861 tagccacaca atataaagca gctgaatccg aattacttaa aaatatatta tcattaccca
    13921 taactccagc cattaaatta ctggagacta aagcaaggaa ataacaacat tgtattgcct
    13981 tcattttaat tactcccttg tctgataatt gtaactccat tccctttagc tttgggatta
    14041 tattttagat aggtgttatt tggtgttccc aagtaactac ttgtattctt tttttcccat
    14101 tccattcata taagaattcg ttaaatcttt gcttgaaaaa tttagactat cataaacatc
    14161 attttctttt gttactttat ttcctttatc gtcagagtaa aaaccgtgag tgtgataatc
    14221 gcctactctc tctgaacctt cagggcattt tatatttcgc aagtctacac tatcattatc
    14281 atttgaactt atcggattca tggggaaata ttcaccttgc ttcttacata ttagtccacc
    14341 atattcttta ttctcgttaa ttgattctcc attacacat
//
LOCUS       DADLTD010000102        14324 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLTD010000102 DADLTD010000000
VERSION     DADLTD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.102"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..619)
                     /locus_tag="JN701_002427"
     CDS             complement(<1..619)
                     /locus_tag="JN701_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY7364855.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSR"
     gene            complement(1007..1753)
                     /gene="cutC"
                     /locus_tag="JN701_002428"
     CDS             complement(1007..1753)
                     /gene="cutC"
                     /locus_tag="JN701_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY7364856.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1767..2333)
                     /locus_tag="JN701_002429"
     CDS             complement(1767..2333)
                     /locus_tag="JN701_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7364857.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2549..4282
                     /gene="argS"
                     /locus_tag="JN701_002430"
     CDS             2549..4282
                     /gene="argS"
                     /locus_tag="JN701_002430"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY7364858.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAN
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4459..4947
                     /locus_tag="JN701_002431"
     CDS             4459..4947
                     /locus_tag="JN701_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAY7364859.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(5067..5459)
                     /locus_tag="JN701_002432"
     CDS             complement(5067..5459)
                     /locus_tag="JN701_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY7364860.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5459..7520)
                     /gene="flhA"
                     /locus_tag="JN701_002433"
                     /pseudo
     CDS             complement(5459..7520)
                     /gene="flhA"
                     /locus_tag="JN701_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(7513..8616)
                     /gene="flhB"
                     /locus_tag="JN701_002434"
                     /pseudo
     CDS             complement(7513..8616)
                     /gene="flhB"
                     /locus_tag="JN701_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8699..9343)
                     /gene="cheZ"
                     /locus_tag="JN701_002435"
     CDS             complement(8699..9343)
                     /gene="cheZ"
                     /locus_tag="JN701_002435"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY7364861.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDRLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9354..9743)
                     /gene="cheY"
                     /locus_tag="JN701_002436"
     CDS             complement(9354..9743)
                     /gene="cheY"
                     /locus_tag="JN701_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY7364862.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9758..10696)
                     /locus_tag="JN701_002437"
     CDS             complement(9758..10696)
                     /locus_tag="JN701_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY7364863.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMQPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10699..11511)
                     /gene="cheR"
                     /locus_tag="JN701_002438"
     CDS             complement(10699..11511)
                     /gene="cheR"
                     /locus_tag="JN701_002438"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY7364864.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11649..12215)
                     /gene="flhC"
                     /locus_tag="JN701_002439"
     CDS             complement(11649..12215)
                     /gene="flhC"
                     /locus_tag="JN701_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY7364865.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12218..12568)
                     /gene="flhD"
                     /locus_tag="JN701_002440"
     CDS             complement(12218..12568)
                     /gene="flhD"
                     /locus_tag="JN701_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAY7364866.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13347..13775
                     /gene="uspC"
                     /locus_tag="JN701_002441"
     CDS             13347..13775
                     /gene="uspC"
                     /locus_tag="JN701_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAY7364867.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13782..>14324)
                     /locus_tag="JN701_002442"
     CDS             complement(13782..>14324)
                     /locus_tag="JN701_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAY7364868.1"
                     /translation="QIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQ
                     HFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELT
                     SALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQI
                     VPRSAESQQRDKVATFPKLA"
BASE COUNT         3683 a         3779 c         3340 g         3522 t
ORIGIN      
        1 tgcgagaagt tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac
       61 tttccagctc atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc
      121 ctttatgaca atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg
      181 cagattcact ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg
      241 cagagtcagt tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagttt
      301 cgaacttatc gtcactgtct attttgccgc taacaaattc atgataaata tctttagatg
      361 cttttaattt agcaaataaa taatccatcc ctgactttgg aatatggcaa tctgcacatt
      421 ccgcacgtat ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt
      481 tactcatcga gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt
      541 gcgccagcaa taacccgccg ccaccaacca caaccgctat cagcaaaaac aataacccaa
      601 tgcgtttttt ccctcgcata gattcctcta aataaattcc agaacaccga aatcattgga
      661 taattaaata cacaacgaat taaccgcttg cataactcgg cacaacactg cctgaaataa
      721 tcgataaaga atatgattta ttacaatgta atcattaatt gctaaggaat aaccctgttg
      781 ttatttagaa tatatatttg tcagttttag caatttaaac cccataatta atgtgaatat
      841 ataaaatatt tttagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat
      901 cgtacgtttt ttatactgct cagggataaa aaaaggcccc tgtcgaaatt gcaggggcct
      961 ggtacgagca agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat
     1021 ggcgttcaat gattcctttc atttcagcaa ccgccgcccc gtctacgaca taacgcgaat
     1081 actcgtccgc gtgtgcatct gatgacatgg acaatccttg attacgataa cgcatcggtg
     1141 aagcttgcca cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt
     1201 gcaggttttc tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa
     1261 taagttccat aatttttgat aaaccttgca gtgcatctga tttttgccct gatgtcagca
     1321 cccgggcgat gcccaattcc gcaagattac tgagtgtatt taaagggtta gcgcacatat
     1381 cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc
     1441 gtggcatatc gacattcccg tcaacatcga gcacgcccgt caccagtcca ggaaaaccta
     1501 attcgcggac tgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa
     1561 aatcaccacc gcgtgggcgg ataatcggat gcacagggat cgtcacccac tggcgcacgg
     1621 atttcagtac acccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa
     1681 ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt
     1741 ccagtaatgc catttttact ccttaattac gccgactgct cgctggcaac gatctcttca
     1801 atggaccacg gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc
     1861 ggtaatcgct catactcacc ttttggcgaa ctggttttca ctgagatccc cggtaggctg
     1921 agtccttcat cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccggc
     1981 agaacgattt caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt
     2041 agctccacaa tggaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga
     2101 caaatcggtc tgccattgat catattttct gacaaaagct cgccacactg ttcaaaaccg
     2161 cgacgccagc gctcagcggt gacgttttga tggcaacgca aagaaatgtg atcggcagtc
     2221 agcggagtga tatccaaccc cagacggcgg gagagttcat ctaatgcgtg ggtaaatcgc
     2281 ggtaaatccg atgcaatatc ctgcagctcg tcgatagatt gccagttcgc cataatcact
     2341 cttcgtcttt cagtaaaagc gttaatttac cctgttgccc tgcgccaacc aaccgctgat
     2401 ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg
     2461 aaaacagcca tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt
     2521 gctcgcttca tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt
     2581 ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc
     2641 agcaaaagtt cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact
     2701 gggtatggca ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat
     2761 cgccagcaaa gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt
     2821 cctggctgaa catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga
     2881 aaaacagacc attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg
     2941 tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca
     3001 caaagtgatt cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc
     3061 atggctggaa aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg
     3121 tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg
     3181 taactacgtg gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact
     3241 ggtcgacatc accatgacgc agaaccagat cacctacgat cgtctcaacg tgacgctgac
     3301 ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga
     3361 tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga
     3421 gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta
     3481 tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga
     3541 tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat
     3601 cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat
     3661 gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc
     3721 caatctgctg gatgaagcct tggaacgtgc acgccgtctg gtggcagaaa agaacccgga
     3781 tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggtg cggtgaaata
     3841 tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc
     3901 gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt
     3961 ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga
     4021 tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc
     4081 ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc
     4141 tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg
     4201 tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat
     4261 tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc
     4321 tgatcaggct tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc
     4381 tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg
     4441 aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc
     4501 caggcttacg cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat
     4561 tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga
     4621 caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat
     4681 atagaacaag tgccacgcac aggtgtaaaa aaacctgata tcgaacaact taacttgctt
     4741 aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca
     4801 aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg
     4861 atcatacaga ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt
     4921 tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct
     4981 attcctgata aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac
     5041 tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt
     5101 acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt
     5161 tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca
     5221 caacgagatt gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga
     5281 taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca
     5341 gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc
     5401 ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt
     5461 atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg
     5521 acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg
     5581 cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct
     5641 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
     5701 cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgg
     5761 gtaatcgccc gtcccaacgc cacgcgcacg acggcggtta attcatgtgg atcgctttgg
     5821 atgggcgcat gttccgccag cgtttcgaga atggtgcgca tatcgcgaat cggtactttt
     5881 tcatcgagga gattttgcag cactttatgc agtgtggtga gcgtgacgac gccaggaacg
     5941 agatcttccg tcagctttgg catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc
     6001 tgacgaccaa acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc
     6061 accgtgctgg cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt
     6121 tcaatccaga tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc
     6181 ccggcagcgg ttccagggtt aatcgccaac cagcgccccg gataagcatc accactgcca
     6241 atctccacgc ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga
     6301 atgtgcacca ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt
     6361 atacggccca acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc
     6421 acttccattc ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggta
     6481 ttattctctg ccatttttac cggtttgggt tcggcaggcg ctttttgttc gcatccgcgt
     6541 atccaccagg ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt
     6601 ccaggcacca agccgagtaa accgagcacg gcggcgctta acaacataac gcttgggtta
     6661 ctgaaaagct gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaacg
     6721 atgacccccg cggcggtaga aatcaccagc gccggaattt gtgccaccag accgtcgcca
     6781 atggtcaata gcgtataact ttccgccgcg tgtcccatgc tcatgccatg ttgcagcacg
     6841 ccgaccagca acccgccgac aacgttaatg accatgatga ggatcccggc gatggcatcg
     6901 ccgcgaacaa acttacttgc cccgtccatt gagccgtaaa aatcggcttc ctgagtcact
     6961 tcggagcggc gttttttcgc ctcatcttca ccaatcaatc cagcgttaag gtcggcgtca
     7021 atcgccatct gcttacccgg cataccatcg agaacaaagc gcgcacccac ttctgcgata
     7081 cgcccggcac ctttggtaat gaccataaag ttgatgatca cgagaatgac aaacaccacg
     7141 atacctatag cgaaattgcc accaacgagg aagtgaccga acgcttcgac caccttccct
     7201 gccgccgccg cgccggtatg cccttccatc aaaatgatac gggttgaagc cacgttaagt
     7261 gccagacgca acaacgtagt aaacaacaga atggtcggaa acgcagcaaa ctcaagcgtg
     7321 cgctgggtaa acatcgccac cagcaacacc atgatcgaca aggcaatatt gaaggtaaac
     7381 aacaggtcga gtatgaatgc gggcagtggc agcaccatca tcgacaagat caacaggatc
     7441 aaaatcggtc cggcaaggat ctgccattgt gtcgatttca ggtttgcggg caggcgcagc
     7501 atcgcggcca gattactcat gggtcggttt ctcgttaata aaatccaggg cttccggcac
     7561 cggaagatga gtaggttgta cagggcgctg tccaccagcc agacgccagc gtttcagttg
     7621 ccagatccag gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc
     7681 aatctccgca tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt
     7741 attttcagcg ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac
     7801 tttcggtgcg ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt
     7861 attgacaatg acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg
     7921 ctgcatctga cggatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc
     7981 acgaatatcc tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac
     8041 gtcaaatccc accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa
     8101 atccatcgca ttacccatgg cggtaatcgg agactcggcc agtaagcgca tcatctgcgg
     8161 ccagtgatgc cagagaaaaa accccgtcac gctgccaacc aagatggttt tcaaaattgc
     8221 tttaagcaac tccgcgccag tctgtgccga gaacatccgt ttaatgcccg gtagcgggtt
     8281 gagtttggaa aacttcggct gcaaggattt tccgctaaat accagccccc ccagcatgac
     8341 cggagaaatg agcgctacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc
     8401 ttctctgatc agcagaataa tctgcccgag gatcagattc gggtctttga taatactgtg
     8461 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
     8521 gaaccagata acacacacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg
     8581 cgggatttag ggcttttgct gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg
     8641 tcatcgcctt atcagaccgc ctgatatgac gtggtcacgc cacatcaggc aatacaaatc
     8701 aaaatccaag actatccaac aaatcgtcca cctgatcctg actggctacc acaccggctt
     8761 tgctggtatc gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact
     8821 cctgttccgg gatgttttcc aacagcacca tcagcaactg gcgttcgatc tcctgaatga
     8881 catccatcat ccgcttaata acctgcccgg tgagatcctg aaaatcctgc gccatcatga
     8941 tttccagcag ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc
     9001 gtgtatctgt taccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat
     9061 cccaacgttg ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg
     9121 cctcaacact gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca
     9181 aacgatcgcg cgcatcgggg atggcttccg ccgcttcggc aatggcctga tccagcccca
     9241 gttcccgcaa cctgtcgcgc agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc
     9301 cagctgaatg ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg
     9361 tccagtttat caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc
     9421 accacatagc cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct
     9481 gcagtcacca ttaacactgg caatgccgac atcgcgccat ccgcacgaat tgttttcagc
     9541 aattccaggc catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct
     9601 gcctgcaact tattgagagc gtcgacgcca tcttccgctt cctcaacatt attgaatccc
     9661 agctctttca gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa
     9721 aatttaagtt ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg
     9781 cactaatttt tgccaacatt tgctggctta cctggctaag atcgaccact tcgcagacac
     9841 cacccatatt gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg
     9901 caagggtcca tgcccccgcc tgacgcatcg ccaacattcc caccgggccg tcgtgaattt
     9961 tgatttgata atttgcgcca ctacgcgcca gttccatatg ccgatcgccc ggcgcaatat
    10021 aggcatgccc cggcaagaca cgttctccgt cttcggcttc tttaaccccg atctggcaaa
    10081 gcttattaag tctgtcggca aaagagcggg tgaaaccggg ctgcatatgt tgggtaatta
    10141 acaatgccgg gctggaaagc ggcaacggtt gcagtacgtg acgaattgcc tcagttccac
    10201 ccgttgaagc accaatcgca atcagttttt cagaactcaa caacggaccc gccttcagcg
    10261 ttgtcggtgc tgacaatggc ttatgtgctg caaggctcgc ctttgctgcc gtacgcacct
    10321 tttcagcaat catttcgcta tacgccagca ttccttcgcg aatacccagt tgcggtttgg
    10381 tgacaaaatc tattgccccc agctccagcg cgcgcagcgt gacttctgat cctttgccgg
    10441 tcaaggaaga aaccatcaca acgggcattg gacgcaaacg cattaatttt tcgaggaaat
    10501 ccagtccgtc catccgcggc atttcaacat ccagcgtcag cacatcggga ttgaatttct
    10561 taatcaagtc acgcgcgacc agcggatcag gcgcggtcgc caccatttcc atgtcgctat
    10621 ggctgttgat gatttctgtc atgatctggc gcatcagtgc cgaatcatcg acagataaca
    10681 ccctgatttt gctcatcgtt aatccttact tagcgcatac accgtctgac cacgcagcgt
    10741 gaagcggcgc tcaaggtggc taaagttttc agagtgaccc gcaaacagca atccgtcggg
    10801 tttaaggagc ggaacaaagc ggcgcaaaat ctcctgctgg gtattttgat cgaagtagat
    10861 catgacgtta cgacagaaga tcgcatcaaa cggccccggc acggtgtact gtttcgccag
    10921 tagattcagc ggggcaaaat caacatagtt cgccagctcc tgacgcacgc gtaccagccc
    10981 ttcatgcggt cccgtccctc gcatgaaata ccgttgcagt tgctgcggcg tcaggttttt
    11041 caactcttca tggcgataaa taccgcttct ggctttttcc agcacttcgg tgtcgatatc
    11101 actggcaaac actttccagc gtccgggcgc ggtgcccaat gtgtcagcca gcgtcatcgc
    11161 aatgctgtac ggctcttcgc cggttgaagc cgccgcgctc catacgcgat actcgccaga
    11221 acggcgacgt gcgtgatccg cgagcagagg gaaatgatgt gcctcacgga aaaatgccgt
    11281 cagattcgtg gtcagcgaat tgataaacgc ctgccactca ccgctgtgct gattagattc
    11341 cagcaagttc agataatgac cgaaatccgt cagtcccagc gaacgcaaac gacgaaccag
    11401 tcggttgtaa accatgtcgc gtttatgatc ggccaggacg atcccggctc gttgatagat
    11461 caattgactt atccgccgaa aactgtaccg agaacaacca ggtaacctaa taagataagc
    11521 acgacatcat ccttccactg ttgaccatga caggatgttc agtcgtcagg cgttaacgcg
    11581 cgattggggc aaaaaaaagc agcggtacgt cgttaccgct gctggcatgt tgcgccacac
    11641 cgtatcagtt aaacagcctg tactctctgt tcatccagca gttgtgggat aatatcggca
    11701 ggattctggg aaagtttacg tctttttact gcccgggatg gcggttgaca taagctgcag
    11761 gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat tgccgccgca gcagttgcag
    11821 ctggaaagtt gcagtaatcc actttcaaca aaccgcacca atgtccaggc acgggttaat
    11881 gccagcagtg gtccttcttc tgcttgtggg cactgttcaa ggtataaacg gtaggctttg
    11941 atcaccgcat cgacgccatt acacaaacct gttttcagta aaaactgcca tgcattacag
    12001 aacatcgaag catgaacgtt ttgttcccag gtcataaacc agtcggttga gaatggcagc
    12061 atgcctttcg gcggtgggct tccgcgcagt tctttataaa gttttatcag gcgtccgcga
    12121 cttaactgtg tttcgctttc cagcatctgc aaacgagcgc ccagggtgat caattcaata
    12181 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
    12241 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
    12301 tttgctggag atcgttaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
    12361 caaaacggaa gtgacaaacc agttgattgg tttctgccag cttaaccatt tgcggaagag
    12421 tcagtgccgc taacgttgtc gccatttctt catttatgcc gagacgaaac atagcggacg
    12481 ctttgtcctg aacaatcaaa cgctgtgcaa gtagtaaata tgacaagttg atgtcataaa
    12541 tgtgtttcag caactcggag gtatgcatta ttcccaccca gaataaccaa ctttattttt
    12601 atgcggtttc accacacccc gtgatgtcgc cgggaagccc cggtaaaaaa taattagcat
    12661 tagaatagtg cgataagctg caataagcag aaccaccttt ttggtttaat atgtccttac
    12721 aaatagaaat gggtctttac acttatctaa gatttttcct aaatcgacgc aactgtactc
    12781 gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatatg tgatgcagat
    12841 cacacaaaac actcaattac ttaacataaa tgtataagcc atacttttgt tttgggtgta
    12901 tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca aaaatgaaac tggaataatc
    12961 gaaatgggat gttgcgcaca gttaaaataa ctcaccgtaa ataatcatct gctataaata
    13021 atcactttca tgcaatacca gataagctat ttttaaacag acacttaccg cacaacaaac
    13081 taattaaata caaagaaagg caacaatcaa cttaatgtta atgaaatgtt ttacatatat
    13141 taaccacttg tttaatgcgg agaatcagta cactattttc ttataattac atttgaaatt
    13201 atatgatcac cgggagtgat tgattaattg tctttactaa taatcagact aatatttacc
    13261 tgtttgaccg agttgggatt gcgtcgtttc tccattagga gtaaagcttt aatgtcacct
    13321 gaagttcaca gaataaggaa caggttatga gctatagcaa tattcttgtc gctgttgcgg
    13381 ttacaccgga aagtcagcaa ctgctggcaa aagcggtatc tatcgccagg ccagtaaagg
    13441 gacacatcag tttaattact ctcgcttccg acccggaaat gtacaatcaa ttagccgcgc
    13501 cgatgcttga agatttacgc agcgtgatgc aagaagaaac gcaaagcttt cttgataagt
    13561 taattcagga tgcggggtat cccgttgaca agacgtttat tgcctacggt gagttaagcg
    13621 aacatattct ggaagtatgt cacaaacatc atttcgattt ggtgatttgc ggtaatcaca
    13681 atcacagttt cttttcgcga gcatcctgct cggcgaaaag agtgattgcc tcaagtgagg
    13741 tcgatgtgct gttagttcca cttacgggtg attaaccgct cctacgcaag ctttggaaag
    13801 gtagcaactt tatcgcgctg ctggctttcc gcgcttcgcg gaactatctg ctttaggtcg
    13861 ctaatgaagc actcctgcca gtggttaata tcgtttttca cgataacgtc cagcatttct
    13921 gcatgacggg aaatacgttc cgccagcgac atagtcaatg cacgatccag cgcagctgca
    13981 acttcgtcac gatcgtaggg gttaacaatt aacgccgacg ttaactcgtt tgccgctccc
    14041 gcaaattgcg aaagaacaag aacgcccggg ttggctgggt cctgagcagc aacatactct
    14101 tttgctacca ggttcatccc gtcacgcagt ggcgtcacta agcccacgtc agagtagcgg
    14161 aatattttca tcagtaattt acggtcaaaa tgctgattca aataataaag cggcgtccag
    14221 cctaattgcc cgtatttacc attaattcgt ccagcttcat tttcgagctg atgacgaata
    14281 tcctgatagg cttgcacatc accacgcgac gttggtgcaa tctg
//
LOCUS       DADLTD010000103        14320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLTD010000103 DADLTD010000000
VERSION     DADLTD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.103"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            6..901
                     /locus_tag="JN701_002443"
                     /pseudo
     CDS             6..901
                     /locus_tag="JN701_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
     gene            complement(1011..2054)
                     /locus_tag="JN701_002444"
     CDS             complement(1011..2054)
                     /locus_tag="JN701_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY7364869.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(2180..2755)
                     /gene="nudK"
                     /locus_tag="JN701_002445"
     CDS             complement(2180..2755)
                     /gene="nudK"
                     /locus_tag="JN701_002445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY7364870.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(2823..4802)
                     /gene="aegA"
                     /locus_tag="JN701_002446"
     CDS             complement(2823..4802)
                     /gene="aegA"
                     /locus_tag="JN701_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY7364871.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            5008..6708
                     /gene="narQ"
                     /locus_tag="JN701_002447"
     CDS             5008..6708
                     /gene="narQ"
                     /locus_tag="JN701_002447"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAY7364872.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            6871..9983
                     /gene="acrD"
                     /locus_tag="JN701_002448"
                     /pseudo
     CDS             6871..9983
                     /gene="acrD"
                     /locus_tag="JN701_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(10082..10141)
                     /gene="ypfM"
                     /locus_tag="JN701_002449"
     CDS             complement(10082..10141)
                     /gene="ypfM"
                     /locus_tag="JN701_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY7364873.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            10522..10878
                     /locus_tag="JN701_002450"
     CDS             10522..10878
                     /locus_tag="JN701_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY7364874.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10882..12009
                     /gene="dapE"
                     /locus_tag="JN701_002451"
     CDS             10882..12009
                     /gene="dapE"
                     /locus_tag="JN701_002451"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY7364875.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            12037..12237
                     /locus_tag="JN701_002452"
     CDS             12037..12237
                     /locus_tag="JN701_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY7364876.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(12347..13045)
                     /gene="ypfH"
                     /locus_tag="JN701_002453"
     CDS             complement(12347..13045)
                     /gene="ypfH"
                     /locus_tag="JN701_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7364877.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(13119..>14320)
                     /locus_tag="JN701_002454"
     CDS             complement(13119..>14320)
                     /locus_tag="JN701_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7364878.1"
                     /translation="HQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQ
                     PIRWAQGCPLEKMVSEALVFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLS
                     GAHYRTSPLDLRRMMDAPGQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRP
                     RGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYL
                     SVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYCL
                     RRDAQALAQWNGETLPVDPLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSE
                     LALPALRGRLQKNASDAQLCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRI
                     TQWQFFH"
BASE COUNT         3354 a         3775 c         3732 g         3459 t
ORIGIN      
        1 ggccgatgct gcctgagttg ctcggcggtt cggcggatct ggctccagca acctgaccat
       61 ctggaaaggt tctgtttcgc tgaaggaaga tccggcgggc aactacattc actacggggt
      121 gcgtgaattt ggcatgaccg ctatagccaa cggcatcgcg caccacggcg gctttgtgcc
      181 gtataccgcc acgttcctga tgtttgttga atacgcccgt aacgccgcgc ggatggcggc
      241 actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggcc tgggcgaaga
      301 tggtccgacg caccaggctg ttgagcaact ggccagcctg cgcttaacgc caaatttcag
      361 cacctggcga ccgtgcgatc aggtggaagc ggcggtgggc tggaagctgg cggttgagcg
      421 ccacaacgga ccgacggcac tgatcctctc aaggcagaat ctggcccagg tggaacgtac
      481 gccggatcag gtaaaagaga ttgcccgtgg cggttatgtg ctgaaagaca gcggcggtaa
      541 gccagatatt attttgattg ccaccggttc agagatggaa atccccctgc aagcggcaga
      601 gaaattagcg ggagaaggtc gcaatgtacg cgtagtttcc ctgccctcga ctgatatttt
      661 cgacgcccag gatgaggaat atcgggagtc ggtgttgcct tctaacgttg cggctcgcgt
      721 ggcggtggaa gcaggtattg ccgattactg gtacaagtat gttggtttga aaggggcgat
      781 tgttgggatg acaggttatg gggaatctgc tccggcggat aagctgttcc cgttctttgg
      841 ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtga aaggtgcctg
      901 atggtgatcg ccggatgctg attgccggac gctgattgcc ggatgcgact ctgacgcgtc
      961 ttatccggcc tacagcgtct tatccggcct acatgtcccc gccattttgt ttaacgggtg
     1021 atccacaacg tgggccaggc gtctggccca tgccagttat cgcaggtggg ttctgcggcg
     1081 taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg
     1141 cttaatccgc gccctttcgc taaggtcacc agttcacgcg atttctcatc aaatgttgcg
     1201 ttcatcagtt ccagttcatt cgtctcctgc ccgttgttga acggcaaacg caaccgaacc
     1261 gggcgcgagg ccagcggatt tttacgcgac acaatccatg ccaaatcaat ggtgttatag
     1321 gcccctgcct cacagctaat catcatcagc gctttatcat cagtcagcgc agtgacattc
     1381 acctcacgac gcaatggatc aagcgagcag cgcagaccat tcatccgcca gtttccataa
     1441 tccagcaaat cgttgcgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccgcg
     1501 acctctttca gcgcaggcgc gggcggtacg ctgagcggcg gttcgtcccc tttcttgatc
     1561 cacgcggttt cactgccaac gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt
     1621 aagccactca gcgaaatggt ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga
     1681 atcatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa
     1741 atccgccact tgtcaccact taacgccaac ggctcgccat ctaacagcag ccgtggcgct
     1801 atctcccctt ctgacgcctc cggcgacttc aatccgccgc gctcaatacg taaaacggca
     1861 tcggtatgcg ccccggcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta
     1921 ttacgcgcca cgcagaagtt ttggttatta caggtgacct gccagtcgga aaacgcccgt
     1981 tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa
     2041 atgcgatagc gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc
     2101 ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat aataaacaac
     2161 gaaatattat tgttattttt cagtccatta aatgtgacgt ttgcaaatag ttaagcaata
     2221 acaccgtctt accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg
     2281 gcagctcgag cacttcaata tcttcatctt cgacaccgcc accggcgtta gcgcgctgat
     2341 tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcct ggcgacatat
     2401 acagttcaaa taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcttctt
     2461 tacgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct
     2521 gcccgctttc attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgg
     2581 tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac
     2641 gtttatggcg gataacttcg ccatctttgc gcgtgagatc gtaggtaatg ttgtgcaggg
     2701 tgaaatagtt atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga
     2761 ctccacagcg cgaaatgaac aattatctta cgctgtgaag tcgggtttgt ctgcgcaggc
     2821 tatcagtgag atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga
     2881 cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc gccaccggcg
     2941 aagattttcg gattggtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg
     3001 ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg cggattgaag
     3061 ccaaacgcca taatcaccgc gtcggctggc atgacaaatt cactgccttc caccggtact
     3121 ggacgccgac gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaatcccg
     3181 caaacgtgac cttgttcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg
     3241 gccccctctt cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt agcttcatca
     3301 cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc
     3361 atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc
     3421 tcttccggta gctcttcgag ccccatcacc tgtttagtgt tggcgatgag gaacggcagc
     3481 gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg
     3541 cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga gacatcttta
     3601 cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt
     3661 gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt
     3721 tctggatggc gatcgtacac cgtcaccgcc acgccattgc gggtcagaac atccgcacag
     3781 gccagccctg ccggacctgc cccgataatc gccacccgct tgtccacttt ggtgacatga
     3841 cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatatagcg ttcaatgttg
     3901 ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct
     3961 tgcggacaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga aagctcgacg
     4021 gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggaatatg gttatgcagc
     4081 gggcaggtcc attcacaaac gctgtgctcg ccgcacttaa ggcagcgcga ggcttcccgt
     4141 tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca
     4201 atcgccagtt tatccggctc gccacgcgcg ggcgttgcct gcatttgttc gactttactc
     4261 attaccggca tttcttgtgc agcggtactg gcatgccacg gttgatgctc ctgacgtgcg
     4321 gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga cgtcagtgac cagttgcagc
     4381 gcgtccgccg ggcaattctc gacacaggca ggaccgtttt cgcgccctgc acaaaggtcg
     4441 catttatgcg ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac gatttgcatc
     4501 gtaccaaacg gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc
     4561 tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca
     4621 tcttcacaat ggtgacaggt cactgcacta cgttgctgct gatgtttgat aaccgtaatt
     4681 cggggatgaa aatggtgttg gctcaggaca tgttgctcat cattgtgagc catgacacag
     4741 gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc
     4801 ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc
     4861 cgacttacag gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa
     4921 actgtggcag ctcaaataat cccatcactg actaaattgc gtttcagcga actggaacat
     4981 taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg
     5041 gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta
     5101 actctggcga gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctgcgtatg
     5161 cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc
     5221 cagttgtttc agcaggcact gcattcaccg gtattaacca atctcaacgt ctggtatgtg
     5281 ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca actggctgga gatgaataat
     5341 cggctcagca agggcgattt gccgtggtat caggccaata ttaataatta tgttaatcag
     5401 atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg
     5461 gcgatttccc tggctggcgg tatcggtatt ttcacgctgg tcttttttac tttgcgccgt
     5521 atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca acgtattgaa
     5581 cacgggcagt tcgactcgcc gccgctggat accagtctgc cgaatgagct tggtctgctt
     5641 gcaaaaacct ttaaccagat gtcgagcgag ctgcataaat tgtacctttc gctggaggcg
     5701 tcagtagaag aaaagacccg cgatctccac gaggccaagc gtcgtctgga ggtgttgtat
     5761 caatgttcgc aggcgctgaa cacaagccag attgatgtgc attgtttccg ccatattttg
     5821 cagattgttc gcgacaatga agcggctgaa tatctggagt taaatgtcgg tgaaaactgg
     5881 cggattagcg aagggcaacc aaacccggaa ttgccgatgc agattttacc ggtgacaatg
     5941 caagagacgg tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg
     6001 ctgcttaaca gcgtttcgtc gatgctggga cgcggtttgt actttaatca ggcgcagaag
     6061 cattttcagc atttattgtt gatggaagaa cgtgcaacca tcgcccgcga attgcacgac
     6121 tcgctggctc aggtactttc ttacttacgt atccagttga cgttactgaa gcgttcgata
     6181 ccggaagata acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat
     6241 gcttatcggc agttacgcga gctgttgact actttccgcc tgacgctgca gcaggcggat
     6301 ctcccctccg cgttgaggga aatgctggat acgttacaaa atcaaaccag cgccaaactg
     6361 accctcgact gccgtctgcc aacccttgcg ctggatgcgc aaatgcaggt gcatttgttg
     6421 caaattattc gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc
     6481 gtcagttgcg tcaccgcgcc ggacggcaat cacacggttt atatccgtga taacgggatt
     6541 ggtatcggtg aaccgaaaga acccgaaggt cattatggtc tgaatatcat gcgcgaacgc
     6601 gcggaacggc tgggtgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg
     6661 agtattagct ttcgctctgc agagggtgag gaaagtcagt tgatgtaatg cctcctactg
     6721 accaaagaat acttgcactt aaggttcagt ataaaagggc atgataattt acattaactc
     6781 cttttttctc cacgattggc tcgtaccttg ccgctacagt gaagcaagtc aagcctacaa
     6841 cgatacgcag aaacacgagg tcctctttta atggcgaatt tctttattga tcgccccatt
     6901 tttgcctggg tgctggcaat cctgttgtgt ctgacaggga ccctagcgat tttttcattg
     6961 cccgttgaac aataccccga tctcgcgcca ccgaatgtgc gagtgaccgc taactatccc
     7021 ggcgcatcgg cccagacgct ggaaaacacc gtgccccagg ttatcgagca aaatatgacc
     7081 ggcctcgata atctcatgta tatgtcatct cagagcagtg gcaccggtca ggcatctgtc
     7141 actttaagtt ttaaagcagg caccgatccg gacgaagccg tgcagcaagt acaaaaccag
     7201 ctgcaatcag ccatgcgaaa gttaccgcag gcggtgcaaa atcagggcgt gacggtgcgt
     7261 aaaaccggcg ataccaatat tctgaccatt gctttcgtct ctaccgatgg ttcgatggat
     7321 aaacaggata ttgccgatta tgttgccagt aatattcagg acccgttaag ccgcgtgaat
     7381 ggcgtcgggg atatcgatgc ctatggttcg caatattcca tgcgtatctg gctggccccg
     7441 gcgaaactca acagtttcca gatgacggct aaagatgtca ctgatgccat tgagtcacag
     7501 aacgcgcaga ttgcggttgg gcaacttggt ggtacacctt ccgtcgataa gcaggcgctc
     7561 aacgccacca ttaacgccca gtcactgctg caaacaccag aacagttccg cgatatcacc
     7621 ttgcgggtga atcaagacgg ctcagaggta aggctgggcg atgtcgccac cgtcgaaatg
     7681 ggggcggaga aatacgatta tcttagccgc ttcaatggta agccagcctc cgggctgggg
     7741 gtaaaactgg cctccggcgc aaacgaaatg gcgacaggga gctggtgctc aatcgtctcg
     7801 acgagctggc gcagtacttc ccgcacggac tggaatacaa ggtggcgtat gaaacaacct
     7861 cgtttgttaa agcctccatt gaagacgtgg tgaaaacgct gctggaagct atcgccctgg
     7921 ttttcctcgt tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg
     7981 ccgtgccggt ggtgttgatg ggaaccttct ccgtacttta cgccttcggt tacagcgtca
     8041 acaccttaac catgttcgcg atggtactgg cgatcggtct gctggtggat gacgcaatcg
     8101 tggtggtgga aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca
     8161 cacgtaaatc gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg
     8221 cggtatttgt accaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct
     8281 ctattaccat tgttgcggcg atggtgctgt cagtactggt agcgatgatc ctcactccgg
     8341 ctctgtgtgc cacactactt aagccactga aaaaaggtga gcatcacggg caaaaaggct
     8401 tttttgcctg gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg
     8461 cgaaaattct ccaccgcagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca
     8521 tggtgttcct gttcctgcgt ttgccgacgt cgttcttacc gctggaagac cgtggcatgt
     8581 ttactacctc ggtacaattg cccagcggtt caacccaaca acagaccctg aaagtcgttg
     8641 agcaaatcga gaaatattac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca
     8701 ccgttggttc tggccctggg ggtaacgggc aaaacgtggc gcgaatgttt atccgcctga
     8761 aagactggag cgaacgcgac agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa
     8821 caaaggcgtt taacaagatt aaagaagctc gcgttatcgc cagcagcccg ccagcaatta
     8881 gcggtcttgg tagttctgcg ggttttgata tggagttgca ggaccacgct ggagcaggtc
     8941 acgatgcgct gatggcagca cgtaaccagt tgctggcgct ggcggcggaa aacccggagc
     9001 taacccgtgt gcgccataac ggcctcgatg acagtccgca gttgcagatt gatatcgacc
     9061 agcgtaaagc tcaggcgctg ggcgttgcta tcgacgatat taacgacaca ctgcaaaccg
     9121 cctggggttc gagctatgtg aatgacttta tggatcgcgg tcgtgtgaag aaagtctatg
     9181 tgcaggcagc tgcgccgtat cgcatgctgc cagatgacat taatctctgg tatgtccgaa
     9241 ataaagatgg cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gagactggct
     9301 cgccgcgtct ggaacgctat aacggttatt ctgcggttga gattgttggg gaagccgcac
     9361 cgggggtcag taccggtacg gcgatggata taatggaatc gttagtgaag cagttgccaa
     9421 acggctttgg tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg
     9481 ctccggcgct gtacgccatt tccttgctgg tggtattcct gtgtctggcg gcgttgtatg
     9541 aaagctggtc ggtgccgttc tcggtaatgc tggtcgtgcc gctgggggtt atcggcgcgc
     9601 tgctggcaac ctggatgcgc gggctggaaa acgacgttta cttccaggtg ggcctgttaa
     9661 cggtcattgg tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga
     9721 accaaaaagg ccacgacctg tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc
     9781 cgattctgat gacctcgctg gcatttatct tcggcgtatt gccaatggca accagcacgg
     9841 gtgccggttc cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcgg
     9901 ccactattct ggctatttac ttcgtgccgc tgttctttgt actggtgcgc cgccgcttcc
     9961 cgctgaagcc gcgcccggaa taagcaataa aaaaggcgac atgctaatgt gtcgcctttt
    10021 tcaactttcc gattaagaac ctgctcagcg ggttcttgct gtttgtactt tgtctcagga
    10081 attacttacg aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca
    10141 taacaacctc tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa
    10201 tttactcgat tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc
    10261 ctgcggtgat aatttgtatg tgatacacag caacatttcg agatattcat acggcatcta
    10321 atactgattt aattctggtt aaaatacaga cagataacaa gatgaatatt cttaatgttt
    10381 acgttaaaaa tgtttaatat tatttaatag ttgttaattt gaatacttcg ataatgttat
    10441 atttcctgat aatcatttgc aggcaaaatg ttttcaccct taaatgagta tttattctca
    10501 tagatcgaaa aaggattcat tatggttaca ctttacggca tcaaaaattg tgacaccatt
    10561 aaaaaggctc gccgttggct ggaagccaat aacatcgact atcgttttca tgattaccgc
    10621 gtcgatgggc tggacagcga attattgaac ggttttatca acgaattagg ctgggaagcg
    10681 ttactgaaca cccgtggtac aacctggcgt aaactggacg aaaccacccg caataaaatc
    10741 accgatgcgg cctctgcggc ggcattaatg actgaaatgc cttcaattat caaacgtcca
    10801 ttgctctgcg cgcctggtaa gcctatgctg ctgggtttca gtgattccag ttatcagcaa
    10861 tttttccatg aggtgtagtc tatgtcgtgc ccggttattg agctgacaca acagcttatt
    10921 cgccgccctt ccctgagtcc tgatgacgca ggatgccagg ctttgttgat tgaacgtttg
    10981 caggcgatcg gttttaccgt tgaacgcatg gactttgccg atacgcagaa tttttgggca
    11041 tggcgtgggc aaggtgaaac gttagccttt gccgggcata ccgacgtggt gccgcctggc
    11101 gacgccgatc gttggatcaa tccgccgttt gaacccacca ttcgtgatgg catgttattc
    11161 gggcgcggtg cggcagatat gaaaggctcg ctggcggcga tggtggtagc ggcagaacgc
    11221 tttgtcgcac aacatcccaa ccatacgggg cgactggcat ttctgatcac ctctgatgaa
    11281 gaagccagtg cccacaacgg tacggtaaaa gtcgtcgaag cgttgatggc acgtaatgag
    11341 cgtctcgatt actgcctggt cggcgaaccg tcgagtatcg aagtggttgg tgatgtggtg
    11401 aaaaatggtc gtcgcggatc gttaacctgc aaccttacca ttcatggcgt tcaggggcat
    11461 gtggcctacc cgcatctggc tgacaatcca gtacatcgcg cagcaccttt ccttaatgaa
    11521 ttagtggcta ttgagtggga tcagggcaat gaatttttcc cggcgaccag tatgcagatt
    11581 gctaatattc aggcgggaac gggcagtaac aatgttattc cgggtgaact gtttgtgcag
    11641 tttaacttcc gcttcagcac cgaactgact gatgagatga tcaaagcgca ggtgcttgcc
    11701 ctgcttgaaa aacatcaact gcgctatacg gtggattggt ggctttccgg gcagccattt
    11761 ttgaccgcgc gcggtaaact ggtggatgcg gtcgttaacg cggttgagca ctataatgaa
    11821 attaaaccgc agctactgac cacaggcgga acgtccgacg ggcgctttat tgctcgcatg
    11881 ggggcgcagg tggtggaact cgggccggtc aatgccacta ttcataaaat taatgaatgt
    11941 gtgaacgctg ccgacctgca gctacttgcc cgtatgtatc aacgtatcat ggaacagctc
    12001 gtcgcctgat gagtggttct gcaagaggaa ataagcatgg actggctggc taaatattgg
    12061 tggattctgg tgattgtctt tttggtaggc gtcctgctga acgtgattaa agatctcaag
    12121 cgcgtcgacc acaagaaatt tctcgccaac aagccggagc ttcccccgca tcgtgatttc
    12181 aacgataagt gggacgatga cgacgactgg ccgaaaaagg atcaaccgaa gaagtaagct
    12241 gagtgctcaa ccgtaggccg gataaggcgt taacgccgca tccggcaatg gtgaacgatg
    12301 cctgatgcga cgctcacgcg tcttatcagg ccaggtaatc tgtcttttac atcatttcaa
    12361 tcacatcatc atcgccaggc ttaccgccac ttagcgcttc gtcgaaataa tgtttcggaa
    12421 tggtataacg caagtgatcc agggcaaatt gcatactgcg attgtcgatc gcatggccta
    12481 aatcctccac gatatccagc gtcacgtcac caccggcact gattaacgct tcctgcgccg
    12541 caaccgcatg cgccagatcg ataaccggat cttcaccgcc atgaatcagg tgaattgtcg
    12601 ttgctgtaga cgcggtttcc ggcaggctcg catagcgtcc gttaaaagca atgacgcgtg
    12661 aagcaaggcc cggttcggct ttaatactct ccagcgccat aatcgcgcct tgagagaaac
    12721 cgattaacgc cgtggcgtta gcccctactc cactctgttt ctgccagtag cgtaccgttt
    12781 caataaacgt cggcatgatg gcatccacac gcgcctggcg attatcttcc gtaataccct
    12841 gcaccgaaaa ccactgacgc cccgccggat taccgctcgg ctccgcgcca ccgacactca
    12901 ccaccagcgc atcaggaaac agcggtgcaa accaactgcc tatttccccc attgccaccg
    12961 agttatcccc gacaccgagg aaaagcagta acaactgttg cacaggttta tccgggcttt
    13021 gaacaacaaa atggtcatgt ttcatggcga actccttaac tgatgtcatt aattttacgc
    13081 cgctggctca caatgaccat gggagaaaat tgaaggagtc agtgaaaaaa ttgccattgc
    13141 gttatgcgat cgcgcagacg ctcagtgcga acatcattaa gtgcgaacag cgcatgcgcg
    13201 gcctcttccc gctgacggac cagtaacatc ttgcggcctg caagtttaag tgtggtacat
    13261 aactgcgcat cactggcatt tttctgtaaa cgcccacgca gcgccggtaa tgccagttca
    13321 ctggtttgta gcagacgcaa taagcaacct aacgacgtta atagcggacg atgagcaaaa
    13381 gcaaaaccgg ccagttcaag ccagtcgtcg tcagaaagca cgatatcgtt tagtggatca
    13441 acaggaagtg tttcgccatt ccactgcgcg agagcttgcg catcgcgacg taaacagtaa
    13501 tgctcacgtt cagccagctg tttacccgca tcactcatcg gtaacagcgc catcgccgta
    13561 tagcaaccac tgctggcttc acgatgatta cccattcgta ccagcacaaa accgcagcgt
    13621 tgccagaaac gccataactc cccggtgtaa ccaaaactca ccgaaagata gtcgaggtct
    13681 tgcgtatatt gcaaagcgct ggcaatgagc tgttgcccaa cgccttcgcg ctgacgagcc
    13741 ggatgaactg ctatccggct gacccgccgt ccacgcaatg tcgctgccag tggattgcta
    13801 ccgtgcgccg ccagcgactg ggccaccaga ttaccccgcg ggcgacgaaa acctgcccat
    13861 accgcctgac tgagttgttg agataatccc ccctcatcca ccagccacag cgctccggca
    13921 atctcgtttt cgccagccgc ctgtaaaaaa tattgccctg gtgcatccat catccggcgt
    13981 aaatccagcg gcgaagtccg gtagtgcgca ccagacaata actgataaac ctttaacggc
    14041 gtttctggct cgcttcgcca taacgtttgt tcaaatgcgg aaatgacgat attgccttgt
    14101 ggtgtatggg tgaagttttc atcgtcaaaa accagtgcct cactaaccat tttttccagc
    14161 gggcatccct gtgcccagcg gatcggctgt tgcagttcaa aacggtgtaa atgcggaaag
    14221 cgagcgcaaa atttcagcaa aaaaccacgt ccggtgcctt cgtagccctg caccgtagtg
    14281 gttaacaacg ttcgaggaaa acgcgatacc agttgatgca
//
LOCUS       DADLTD010000104        14198 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLTD010000104 DADLTD010000000
VERSION     DADLTD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.104"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(92..388)
                     /locus_tag="JN701_002455"
     CDS             complement(92..388)
                     /locus_tag="JN701_002455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364879.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            672..2303
                     /gene="oppA"
                     /locus_tag="JN701_002456"
     CDS             672..2303
                     /gene="oppA"
                     /locus_tag="JN701_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY7364880.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTTSPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2389..3309
                     /gene="oppB"
                     /locus_tag="JN701_002457"
     CDS             2389..3309
                     /gene="oppB"
                     /locus_tag="JN701_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY7364881.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3324..4232
                     /gene="oppC"
                     /locus_tag="JN701_002458"
     CDS             3324..4232
                     /gene="oppC"
                     /locus_tag="JN701_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY7364882.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGTNSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4244..5257
                     /gene="oppD"
                     /locus_tag="JN701_002459"
     CDS             4244..5257
                     /gene="oppD"
                     /locus_tag="JN701_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY7364883.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            5254..6258
                     /gene="oppF"
                     /locus_tag="JN701_002460"
     CDS             5254..6258
                     /gene="oppF"
                     /locus_tag="JN701_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY7364884.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6311..6640)
                     /locus_tag="JN701_002461"
     CDS             complement(6311..6640)
                     /locus_tag="JN701_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY7364885.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6675..8135)
                     /gene="cls"
                     /locus_tag="JN701_002462"
     CDS             complement(6675..8135)
                     /gene="cls"
                     /locus_tag="JN701_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY7364886.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8278..8451
                     /locus_tag="JN701_002463"
     CDS             8278..8451
                     /locus_tag="JN701_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY7364887.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8506..9759)
                     /gene="kch"
                     /locus_tag="JN701_002464"
     CDS             complement(8506..9759)
                     /gene="kch"
                     /locus_tag="JN701_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY7364888.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10059..10355)
                     /locus_tag="JN701_002465"
     CDS             complement(10059..10355)
                     /locus_tag="JN701_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY7364889.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10570..11298
                     /gene="tonB"
                     /locus_tag="JN701_002466"
     CDS             10570..11298
                     /gene="tonB"
                     /locus_tag="JN701_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY7364890.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="JN701_002467"
     CDS             complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="JN701_002467"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY7364891.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11841..12380)
                     /locus_tag="JN701_002468"
     CDS             complement(11841..12380)
                     /locus_tag="JN701_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY7364892.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12410..13153)
                     /locus_tag="JN701_002469"
     CDS             complement(12410..13153)
                     /locus_tag="JN701_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY7364893.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(13181..13270)
                     /locus_tag="JN701_002470"
                     /pseudo
     CDS             complement(13181..13270)
                     /locus_tag="JN701_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13510..14148
                     /gene="ompW"
                     /locus_tag="JN701_002471"
     CDS             13510..14148
                     /gene="ompW"
                     /locus_tag="JN701_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY7364894.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT         3828 a         3317 c         3472 g         3581 t
ORIGIN      
        1 cgcagttatc aaaagcaaaa ggaataggta aaaatattct tctcaaatta cagttagtta
       61 taaggatttt cttaactgct tctcctcacc atcatgttat tttcgccaca tcataatcct
      121 gggcttgctg aagaataatt gaaatgatat tagtaattcc actgcctttg gtagaggaaa
      181 gtgctaaata ataatcaatt gttaaattat tgtgcatttc aatactgaaa ctgtaatcag
      241 aaaagataga catgcttagc caatctctat ttgattgaat tgaaagatgt ttgttaaggc
      301 atggatgcaa gctatagatt ctgatacggt caataaaaga gaattgctta acaattttgc
      361 aaaatgtatt ggcgagtaag aaccgcattt agtactttcc gggcaactgc cagacgattc
      421 tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata atgtgtctcg
      481 acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca gaataaataa
      541 agtcggtgat agcaatccgt aacgataaag taacctgaca gcagaaagtc tccgagcctg
      601 tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat aatgagggag
      661 tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc gttctggctg
      721 cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg
      781 aaaaacaaac gctggtacgt aacaatggtt cagaagttca gtcattagat ccgcacaaaa
      841 ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta ctggtcagcg
      901 atcttgacgg tcatccggca cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag
      961 tctggacctt ccatttgcgt aaagatgcga aatggtctga tggtacgcca gtcacagcac
     1021 aagactttgt gtatagctgg caacgttctg ttgatccgaa cactacttct ccgtatgcca
     1081 gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga
     1141 ttaccgatct cggtgtgaaa gctattgatg atcacacatt agaagtcacc ttaagtgaac
     1201 ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg ccaaaagccg
     1261 ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc aacggtgcct
     1321 ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc ccgacctact
     1381 ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct tctgaagtta
     1441 ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac ttataacaac atgccgatcg
     1501 aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat ccatacctgt
     1561 gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta
     1621 ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg
     1681 acatgcccgc ctatggttac actccaccgt atactgatgg cgcaaaattg actcagccgg
     1741 aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag
     1801 cgggttatac cgcagacaaa ccgttgacaa tcaacctgtt gtataacacc tccgatctgc
     1861 ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt gtaaacgtca
     1921 aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggt acttttgatg
     1981 tggcccgtgc aggctggtgt gctgactaca acgaaccaac ttccttcctg aacaccatgc
     2041 tttcgaatag ctcgatgaat accgcgcatt ataagagccc ggcctttgac agcattatgg
     2101 cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact aaagcagaac
     2161 aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg
     2221 tgaaaccgtg ggttggtggc tataccggca aagatccgct ggataatacc tatacccgga
     2281 atatgtacat tgtgaagcac taatggcaat acgtggggca ggagtgtcct gctccacggt
     2341 gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat gttaaaattt
     2401 attctacgtc gctgtctgga agcgattccg acgctattta ttcttattac tatttcgttc
     2461 tttatgatgc gcctcgcgcc gggaagccct tttaccggcg aacgtacttt accgccagaa
     2521 gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac acagtatttc
     2581 agctacctga aacaactggc gcacggtgat ttcggtccat cgtttaaata taaagattat
     2641 tcggtcaatg acttggttgc atccagtttt cccgtttctg ccaaactggg agccgcagca
     2701 tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac
     2761 accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat ccccagtttt
     2821 gtggttgcac cattattagt catgatattt gcgatcattt tgcattggct gccgggcggt
     2881 ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt gtcactcgct
     2941 tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt acactccaat
     3001 tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt acgtcacgca
     3061 ttaaaacctg ctctgttacc cgtgctctct tatatgggcc ccgcatttgt cggcattatt
     3121 accggttcta tggttatcga aaccatttat ggtttgccgg ggattggaca actgttcgtt
     3181 aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct ggttggtgct
     3241 ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga cccgaaaatc
     3301 cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag acgctggaaa
     3361 atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt
     3421 ttattcataa ccgtgcggcg gttgccagtc tgatggtgct ggtgctgatc gcgttatttg
     3481 taatcctggc accgatgctt tcccagtttg cctatgacga tactgactgg gcgatgatgt
     3541 ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc ggtcgcgacc
     3601 tgcttgtgcg cgttgcgatt ggtgggcgta tctcactcat ggtcggtgtt gctgcggcac
     3661 tggtggctgt ggtcgtcggg acactttatg gttcgctttc cggttatctt ggcggtaaag
     3721 tggattcggt aatgatgcgt ctgctggaaa tcctcaactc cttcccattc atgttcttcg
     3781 tcattttgct ggtgaccttt ttcggtcaaa acatcctgct gattttcgtg gcgataggca
     3841 tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca
     3901 aagagtttat tgaggcggca caagttggcg gtgtatcgac atcgggcatt gttattcgcc
     3961 acattgtgcc gaatgtactc ggtgtggtgg tggtctacgc atcacttctg gtgcccagca
     4021 tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag ccgttaagca
     4081 gctggggtgc attgctgagt gatggcacga actcgatgga agtctctcca tggttattgt
     4141 tgttcccagc gggattcctc gtggtgacgc tgttttgttt caactttatc ggcgatggct
     4201 tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg taattgaaac
     4261 tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga aagatttgcg
     4321 tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttga atttttccct
     4381 acgtgccgga gaaacgctgg gtattgtagg tgagtctggt tcgggtaaat cgcaaactgc
     4441 atttgcgttg atgggcctgt tggctgccaa cggacgtatt ggcggatcgg caaccttcaa
     4501 tgggcgtgaa atcctcaatt tgccagagca tgaactcaat aaactgcgcg ctgaacaaat
     4561 ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg tcggtgagca
     4621 gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt ttgaagagtc
     4681 ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga aaatgtaccc
     4741 gcacgagttt tctggcggca tgcgtcagcg agttatgatt gcgatggcat tgctatgtcg
     4801 acctaagctg ctgattgcgg atgaacccac tacagcgctg gacgtcaccg tacaggcgca
     4861 gatcatgacg ctattaaatg aactgaaacg ggaatttaat accgccatca ttatgattac
     4921 ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt acgccgggcg
     4981 cacgatggaa tatggcaacg cgcgcgatgt cttttatcaa cccgttcatc cttattctat
     5041 cggtttgctc aacgcggtgc cgcgtctcga tgcggaaggt gaaacaatgt tgaccatccc
     5101 gggtaatccg ccaaacctgc tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc
     5161 gcatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc ttggccgtct
     5221 gtgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaaccga aggaagaaaa
     5281 gtcctccttg aaatcgccga tctgaaagtg cactttgaaa tcaaagatgg caaacagtgg
     5341 ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg tcacgcttcg cctgtatgaa
     5401 ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt tgctcgcgcc
     5461 atcattggcc tggttaaagc gaccgacggt catgttgcct ggttaggtaa agagttgctg
     5521 ggcattaagc ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat tttccaggat
     5581 ccgttggcat cgctaaaccc gcgcatgacc atcggcgaga tcatcgctga accactgcgt
     5641 acttatcatc cgaaaatgtc acgtcaggaa gttcgcgagc gcgtgaaggc gatgatgctg
     5701 aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc cggtgggcag
     5761 tgccagcgta tcgggattgc tcgtgctctt attcttgaac cgaagctgat tatctgtgat
     5821 gagccggtgt cggcgctgga cgtgtcaatt caggcgcagg tggtcaacct gctccagcag
     5881 ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc cgtggtaaaa
     5941 cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact ggggacctat
     6001 gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc ggcagtcccc
     6061 atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg ggaattaccg
     6121 tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat tgccggtccg
     6181 gagtgcgcca aaacacgtcc tgtgctggaa gggagtttca gacacgccgt ttcttgcctg
     6241 aaagtcgatc cgctttaaac acaataaggg ctgacagttg tcagcccttt ttcacgccga
     6301 aagcgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt tcgcggcata
     6361 acagaatgcg ggcgaaaaca tcgttgattt caccatcctc actgtccgcc agaccaatca
     6421 ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac atgttcctgc cagtcttcca
     6481 ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac agcagaacat
     6541 cagctgggtc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg
     6601 tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc tgtttaacct
     6661 ctgttggaga cgttttacag caacgggctg aagaagtaaa acagtcgctc ggcgacacgt
     6721 tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg cgaaatataa
     6781 tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc cagggtaatc
     6841 tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag acttagttcg
     6901 ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata aattttaacc
     6961 ccagcagcaa gaagttccgt aaagaatgcg cgactggccc agccgaccag catcgagtca
     7021 ttttttcgcg gaaggataat actgacatcc accccgcgct gtgccgccgt gcaaatcgca
     7081 tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata ttcacgcgcc
     7141 gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc ggggccagaa
     7201 gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg gcataatatt gacatctggt
     7261 ggtggcggca gaatacgttt tccggtttca atctcccagt cgcaggaata aataatcccc
     7321 atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg ccctacgccc
     7381 gcatcttgtt tgaagtagcg aggatcgacc atattcatgc tgccggtgta cgcgatgtaa
     7441 ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc
     7501 atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat tagctcgggc
     7561 cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg gcaatgaatg
     7621 ccgcgtcgcg cagcggccat taatgattca gccacctggt ccgccatgcc gccgggctgc
     7681 cagatataaa acacaatctc aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc
     7741 tgcatcacat catctgactc ggtcatcagt tgtagctgat tccctttgac cccagcgatc
     7801 ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt ttcttcggcg
     7861 aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg ccacatcgct
     7921 ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag ataggcaata
     7981 attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga gggaactgcg
     8041 cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta tcccagaatg
     8101 gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc
     8161 attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg aaaagcactg
     8221 gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt agttaacatg
     8281 aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa
     8341 tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat caggaagtgc
     8401 attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta aatgtactgg
     8461 gcaccattgc ggtgcccaat aagtttactc ctccatcttt aaactctatt tttgcgccga
     8521 ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatgc catgaccgga
     8581 gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta aaactcgcgc
     8641 cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta ccatcttaat
     8701 cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac tgctcatatc
     8761 ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg ccagaatggc
     8821 caggcaacga tcgattcccg ctttctttaa tactgaactg tcattactgt caccggggat
     8881 aacatcagcg ttatcgccta agcgttgctc aagttgcttg atatcatctt caggcaagtt
     8941 gctgataacc gttacgtttt gtccgcgttg attcagttgc agaatcgtat tgatggcgag
     9001 aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat tgtttccttt
     9061 tacaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca tagatgtggc
     9121 aaaaacggta ataccggaaa taatgaccga aatagtgaac aatcgtgctg attcagaaac
     9181 agggacaata tcgccatagc cgacggttga catggtttct atcgaaaaat agaacgcggt
     9241 catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac cgtaggttga
     9301 gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg cggcgctact
     9361 gtgggagaag tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc aaaatccaat
     9421 actaaatttc aaccagggat aaaaatgcag ggtataaatt agcgctatca acaacaaaat
     9481 gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt tcaggacaag
     9541 aaagacgccg agcataaata gtggggcgtt tgccagcaag gagagatcca actctgaaaa
     9601 tgctttgata tggaaaatat ctagcaaatt gaccgacata ccatagattg ttttaaaaat
     9661 aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg tgccgtaggg tcacccataa
     9721 atttgttgct gtttgtttga atgtagccca gtgactcact gatacctctg taaccacaat
     9781 tttgtttaat tatcttagcg tgcacatttt ctgaacatac atgcagcgcg tgaatgtgtt
     9841 aaacaatagt agatgacttt atccgcagta atcaaaggtt ttgagcgagc aactgtacct
     9901 caggaaaggt catttttcct gaatatgctc acatcatata aagaaataca gataaagtta
     9961 ttatctgctt gtggtggtga atgcactgac cggctataag gaaaggccaa acaagaacac
    10021 ggttgcaaaa accgtgccct taaatattga atctctattc agaacacttt cttaaatggt
    10081 ttcactgaaa cgtgttcata gactcctgcc gctacgtacg ggtcagcatc ggcccaggcc
    10141 tgagctgctt ccagcgattc aaattcagca ataacggttg agccagtaaa tcccgcagcc
    10201 cctggatcgt tactgtctac cgctggcatt ggaccagctg tcaacaaacg accttcatca
    10261 tgcagtaact gtaaacgtgc taaatgtgcc ggacgaacgg aaaggcgttt ttcgaggcta
    10321 tcagctttgt cttgagcgta aataacatac aacacgggca caactccttg ttcgggaaag
    10381 ttgtaaagta cgttatttga aagggcgaag atctgcaacg gaaagatgat gtctttgtta
    10441 agaccatgca taaagtaagg ttaattacgg caaaaatgac attttcactg atcctgatcg
    10501 tcttgcctta ttgaatatga ttgctatttg catttaaaat cgaggcttgg tttttctact
    10561 gaaatgatta tgacttcaat gacccttgat ttacctcgcc gcttcccctg gccgacgtta
    10621 ctttcggtct gcattcatgg tgctgttgtg gcgggtctgc tctatacctc ggtacatcag
    10681 gttattgaac tacctgcgcc tgcgcagcca atttctgtca cgatggttgc gcctgctgat
    10741 ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa
    10801 cctgagccga tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg
    10861 aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc
    10921 aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata cggcactggc acgcctgaca
    10981 tcaagtacag caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc
    11041 gcattaagcc gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg
    11101 caggttaaag ttaaatttga cgtcacgccg gatggtcgcg tggataacgt acaaatcctc
    11161 tcagccaagc ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt
    11221 tatgagccgg gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc
    11281 accaccgaaa ttcagtaagc agaaagtcaa aagcctccga ccggaggctt ttgactatta
    11341 ctcaacaggt aaggcgcgag gttttccttc aggatcaacc gcgacatact taaataatgc
    11401 ttctgtcgct ttatagcgtt gcccgattgg ttcagacgct acttttttca cccacacttc
    11461 aatattaatg ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacaccac
    11521 atcgccgacc gcaaccggcc gtaagaaagt cattccttca acccgcacgg tcactacgcg
    11581 accgtgggca atttctttcg ccagaatagc gccgccaata tccatttgtg acattaacca
    11641 accaccaaag atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac
    11701 aagatcgccc tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa
    11761 tcacaggcgc gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc
    11821 ccgaaggact ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag
    11881 ataccgctta acaatgtgaa gattaaggta agggcggtca gtccaaagac tttaaagttg
    11941 acccaaatat tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata
    12001 aagaaaacag cccaggccag attcagcttc gaccataccg gttgcggcag cgtgagttct
    12061 ttacccagca tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggct
    12121 cccgcaaaca gagcataaat gactgtaacc ttccatttaa taaactcatc attgtggaag
    12181 aacagcgtca agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca
    12241 accttacgaa agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgcc
    12301 gcagtagccg catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa
    12361 aaatcaagaa actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa
    12421 tcaacatata cagacggaac agatagatga gcaatacggc tgaaatcagg ttgctcaagg
    12481 tgttcgccag tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca
    12541 gcaacagtgt ttttgccagc aaccagctca gtactgcggg tgccaccaga cgcatattcg
    12601 cccaagtcag ccgcatactg ctacgcatcg aggcaaaaat gcccattttg tcctgtacca
    12661 gcatcaccgg tgccagggcc agtaaaatgg ccatgataat tcccggaacg accactagca
    12721 tgatgccaat ctgtactaaa agggtagtca gaaaaatcag aataaataac tttggcaata
    12781 tcggtgcact ggcaccaata gctcgcagcg cactgactct ctgacccgca gacaccagct
    12841 ggataattaa tattacgcct ccggcgagaa tggcgttacc gattaatcct gaaaaagtgg
    12901 acgccgctga agcctgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt
    12961 cgaacaaccc actactgccg ctaacgggca cgccgtcatt gagctgcgca agctgtgcat
    13021 cactgggtga gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca
    13081 acagaatggt cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg
    13141 ccgtgataga catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta
    13201 ccctggttaa gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa
    13261 gcggaggtaa ttgctggcct tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa
    13321 tatttgcgta gcgaaaatat ttaaaaattg atttaaatca cgttaaccag gattctcaat
    13381 gcaacttcta aattaatcca gatcaataaa gggtgaatta tcatatgtaa tgtgatctat
    13441 gtaggatcat ttgttactcc aatgtaggta tattcgtcac gtttttataa ccataacgac
    13501 ggagcggata tgaaaaagtt aacagtggcg gctttggcag taacaactct tctctctggc
    13561 agtgcctttg cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt
    13621 ccaacagaag gtgctggtgg tacgttagga agtctgggtg gattcagcgt gaccaataac
    13681 actcaactgg gcttgacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg
    13741 gcagcgacgc cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt
    13801 caccatctgc cgccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc
    13861 cgtccttacg ttggggcagg tattaactac accaccttct ttgataatgg atttaacgat
    13921 catggcaaag aggcagggct ttccgatctc agtctgaaag attcctgggg agctgccggg
    13981 caggtggggg ttgattatct gattaaccgt gactggttgg ttaacatgtc agtctggtac
    14041 atggatatcg ataccaccgc caattataag ctgggcggtg cacagcaaca tgatagcgta
    14101 cgcctcgatc cgtgggtgtt tatgttctca gcaggatatc gtttttaatt ccgcacaaaa
    14161 acgaccccgt aatatacggg gtcaataagg acatggca
//
LOCUS       DADLTD010000105        13910 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLTD010000105 DADLTD010000000
VERSION     DADLTD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.105"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JN701_002472"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JN701_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY7364895.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JN701_002473"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JN701_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY7364896.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2659..5772)
                     /locus_tag="JN701_002474"
     CDS             complement(2659..5772)
                     /locus_tag="JN701_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY7364897.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="JN701_002475"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="JN701_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HAY7364898.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="JN701_002476"
     CDS             7014..7292
                     /locus_tag="JN701_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364899.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="JN701_002477"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="JN701_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY7364900.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEFKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="JN701_002478"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="JN701_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY7364901.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="JN701_002479"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="JN701_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY7364902.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="JN701_002480"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="JN701_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY7364903.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="JN701_002481"
                     /pseudo
     CDS             10284..10931
                     /locus_tag="JN701_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(10973..11503)
                     /locus_tag="JN701_002482"
     CDS             complement(10973..11503)
                     /locus_tag="JN701_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7364904.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="JN701_002483"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="JN701_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY7364905.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12932..13696)
                     /locus_tag="JN701_002484"
     CDS             complement(12932..13696)
                     /locus_tag="JN701_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364906.1"
                     /translation="MSIDFTPGIINTYHGNIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFT"
BASE COUNT         3856 a         2966 c         3083 g         4005 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacgcgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggtgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca agagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacctggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgc tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgct
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcccctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcaac ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcaac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgaactctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctaattg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     8581 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgatttgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctatatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgtg aggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtttgac gacggcagcg gatatctgga tgaccgccgc tataggtatg gttattggca
    10621 gtggtatgta cgaattgggt atgtatggtt cagtgatgac cttgctggtg ctggaagtct
    10681 tctatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatac ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa acggatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagatctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagtc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgttat tttcagtttc tccagaatat
    11101 cctccgttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacagccgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcgatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gcactttccc caggcagcgt ttaatattaa
    11281 agggggagaa aataatcaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacaaa ggcattctga tgcaacattc
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttgctcaaa atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaatt ttataatcag tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tgctgtgtat taattttatg taatgcacga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccatag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca taagtatcca tcgccccata atataatcct ttgttttaaa
    12421 attacataaa atctattttt attcaataat tataaagtgc taataatcaa tattatggta
    12481 ttatattctg ataatgattt gataagcgaa ttcttttgcc agatggtatt tctatttcca
    12541 ccccataatt tatatctttt tcattaggaa gtggcctctt taaataaggc gtgtgtgaca
    12601 ttttttttgg gttaagcgtc tcgaattgaa atacttcgaa ctgatattca gacatttctg
    12661 cccatgtttg aattttattg tatcgagaga gtgtcgtaga ggctttaact tgcttataac
    12721 attgtttttg gaattcttgc tgatatttta atatgtcatt gtatgaaaga ttggttatcc
    12781 tggcctctaa aaatttaatt attgtcgaag caatttccgc ttttgctcgt aaaaagttgt
    12841 cattaatggt taataatgtt gaaaataaac cttcgatttg tttgtcaaga tattgtccat
    12901 ctttatataa tttggggggg tagggtgttc tttatgtaaa aatagtgtca cctaaatttg
    12961 cgtcagtgaa atttatatta agtagatctt cgaaataaaa ccttgacaag gttagaacga
    13021 acccggaaag gttctgtcct gtcatattga aatcaataaa gttggttccg gcaaagactt
    13081 tcatcctgtt tttattttca ctgtgctcaa tatataactt cgtcattttt tgatacaggc
    13141 tttgaatatc ttttatatta gttggtaagt cagctcgttg ttcagcaata aaattagtat
    13201 ctgaaaacat ggtttggaca ctgaatgtta tgggtattcc ctcttctacg ctaattttag
    13261 ttatgattgc cttgtcgggt ttaggattag cgacaacaat gattcgacat tcagcacttt
    13321 tgtttttttc atatctaatg ttattaatct gattaagtat tgattgtgtc tgttgcgtaa
    13381 gtggtgtcga acttatcgtt tcaatgttat taataagact tacaagttga accacaactt
    13441 ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt tgttggagtt
    13501 gttgttgcgc taaatcaaat tgttcagggt tgtatccttc tccagagaat gtggaattaa
    13561 tgggttgttg ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc gttttaacat
    13621 tatcggtatt tgttgtgcag ttataaatat tgccgtgata tgtatttatt atacctgggg
    13681 taaagtcaat tgacataaca ttccttattc atcatctgtt ttatgctaag gtttttaaat
    13741 tggataatta gaacaggaat ggattttctt ctacacacaa taaatataat gcgttatatc
    13801 ttcctgatat aattaccttt aaattccctt tcatttccac ccggtaatat ttgtgtataa
    13861 aaaaagcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag
//
LOCUS       DADLTD010000106        13875 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLTD010000106 DADLTD010000000
VERSION     DADLTD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.106"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            17..153
                     /locus_tag="JN701_002485"
     ncRNA           17..153
                     /ncRNA_class="other"
                     /locus_tag="JN701_002485"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(244..699)
                     /locus_tag="JN701_002486"
     CDS             complement(244..699)
                     /locus_tag="JN701_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7364907.1"
                     /translation="MVAEGILLERKEWLITRCEKMYFDNLEKWFGHYCHIHCQVSLGR
                     LINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKIVCVIELDDPSHERADRIIR
                     DRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPLVAATKSEPVH"
     gene            complement(1511..2575)
                     /gene="phoH"
                     /locus_tag="JN701_002487"
     CDS             complement(1511..2575)
                     /gene="phoH"
                     /locus_tag="JN701_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY7364908.1"
                     /translation="MVTSCTGHVLDNQRATTRGVFSSGSHLVTLHFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
     gene            complement(2921..4192)
                     /gene="efeB"
                     /locus_tag="JN701_002488"
     CDS             complement(2921..4192)
                     /gene="efeB"
                     /locus_tag="JN701_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY7364909.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4198..5325)
                     /locus_tag="JN701_002489"
     CDS             complement(4198..5325)
                     /locus_tag="JN701_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY7364910.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(5383..6211)
                     /locus_tag="JN701_002490"
                     /pseudo
     CDS             complement(5383..6211)
                     /locus_tag="JN701_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            6247..6549
                     /locus_tag="JN701_002491"
     CDS             6247..6549
                     /locus_tag="JN701_002491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364911.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPGDINDYS
                     RVERWDCLQRTMCSCRMPRKRHIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(6880..8388)
                     /gene="putP"
                     /locus_tag="JN701_002492"
     CDS             complement(6880..8388)
                     /gene="putP"
                     /locus_tag="JN701_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HAY7364912.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8547..8756)
                     /locus_tag="JN701_002493"
     CDS             complement(8547..8756)
                     /locus_tag="JN701_002493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364913.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8811..12773
                     /gene="putA"
                     /locus_tag="JN701_002494"
     CDS             8811..12773
                     /gene="putA"
                     /locus_tag="JN701_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7364914.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPLDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12813..13451)
                     /gene="rutR"
                     /locus_tag="JN701_002495"
     CDS             complement(12813..13451)
                     /gene="rutR"
                     /locus_tag="JN701_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY7364915.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            13739..>13875
                     /locus_tag="JN701_002496"
     CDS             13739..>13875
                     /locus_tag="JN701_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY7364916.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     AL"
BASE COUNT         3436 a         3782 c         3427 g         3230 t
ORIGIN      
        1 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
       61 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
      121 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
      181 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
      241 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
      301 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
      361 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
      421 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
      481 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
      541 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
      601 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gagtgattaa
      661 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
      721 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
      781 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
      841 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
      901 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
      961 atttcattac caatgttact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     1021 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     1081 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     1141 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     1201 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     1261 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     1321 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     1381 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     1441 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     1501 gataacacac ttaactgtat gcatgcagcg tacgttggca aagtgccgaa cgtacgcaat
     1561 cctcttttcc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     1621 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     1681 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     1741 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     1801 acggcgcgat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     1861 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     1921 tatcaccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     1981 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     2041 ttccgcaccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     2101 agtgcaattg cgcttcattt cgcgcgagga tgggcgaggt atcgcgactg tcgcgggcca
     2161 taccaatggc ttctacgccg ctcatctgca caagcgaggt gaccgattct tcttcacgct
     2221 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     2281 ctgctttttg tcttcccatg gagagcacct tgagttgttt gtattcatca cacgcgccgt
     2341 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     2401 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     2461 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     2521 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     2581 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     2641 agatctcgca ttttattgca acaatatatt tacttatatt taactataaa acactatttc
     2701 agtgacatta gtttctactg gaaagatgac agagtgatga cagtgatgaa aaaagctgtg
     2761 tgctttcagc aggatttgca tcaggcatca gtgcacgatc gccggatgcg gcgtgaacgc
     2821 cttatccagc ctaccaaacc cgcactgatt gtaggcatga taagacgcgc aagcgtcgca
     2881 tcaggcatct gcgcacaaat gccttatccg cctaaaaaca ttaaacccgc aataacgcgc
     2941 ttccgagata atcgttcgcg tccttcactc ccggcagcga aaaaaaataa ccgccgccga
     3001 taggtttaac gtattcctcc agcgcttcgc cattgagcct tttttgtact gtcaggaagc
     3061 ctttttccag atcgtgttgg tagcagacaa acagcaaccc catatccagt tgtccggagt
     3121 tggtgacccc caacgaataa ctgtagccgc gacgcagcat caggctggac tcactctccg
     3181 ccgtgcgggg attcgccagc cggatatggc tgtccagcgc gatcaccttc ccttccgggt
     3241 cgctggcgta atcaggcaca tcatgctcat gctgcattcc cagcggcgca ccggtttgct
     3301 tatcacggcc aaaaatcgtc tgctgttctt tcagcggcgt tctgtcccaa aattccactc
     3361 gaaactgaat caagcgtact gcctgatagc tgccaccgat tgtccacgca ggctcctgct
     3421 gatctgccgt tacccacacc actttttgca tcaacttatc attctggcta tcgggattgg
     3481 cagtgccgtc tttaaagccc agcaaattaa tcggcgtctc tttgccttta ctacgcgctg
     3541 cgtgatcgga aataaaccct tcccgcttcc agcgcacgct gagcaaatcc ggcgtgtgtt
     3601 tgatgatatc gcgcagcgca tggatcaccg tgtcctgggt gttggcgcaa atttgtagca
     3661 acacatcacc atggcataac gccgcatcca gcgagtcgtt ggggaaacgc gtcatcttct
     3721 gcaacttttt cggcatctgt ggcgcaaggc caaaccgctc atcaaacaat gagtgaccca
     3781 ccgataacgt gatggtgaga ttatcgggcg caatgtagcc gccaagaatg ccggaatcga
     3841 gtggtggcag gcgcggattt ggcgtttctg gtgccgctcc gccctgagtc agaaaagcaa
     3901 aacgctgagt caacaagcga aacaaccgtt caagatcggc tttatcgctg gcaagcacat
     3961 caaacgccac cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc tgatgctcac
     4021 cataaaacgg ctgtttctca ttgcgtgcat ccggtgaaag cgtacccggc gcactttgcg
     4081 ttttttgtgc atgagcgacc ggacaacttc ccgccagcgc cagcgcacct atcactttca
     4141 gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc tttatactgc ataacgctta
     4201 atccagtccc agcacaccgc gaagttgcgc cagatcttcc gcctgcgcag taatcggtcc
     4261 tttcagtgca ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac cgtctttagt
     4321 acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt ttgccagcag
     4381 ttccgggttg gctttttgca gttgtggacg cagcaaatcg acaattttct gcgagccttc
     4441 aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt caccgctaat
     4501 tttgctggct gccacttcct caatcagtcc ggctgcgccg ccgaccactt ttgaaggtgg
     4561 gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tctgtataaa gctgctcagc
     4621 gtactgatcc atccctttgg tggtgttgtc gccaaacaat gctttttcca gacggtggaa
     4681 accagtgaat tttgggtcgg cggctttttg ctcgtaatca tcttcacggg cgtcaatgct
     4741 gccatccaga tcggagaaca gttcagcaat cggttcaatg cgctcatagt gctggcgcgt
     4801 cggcgcatac agtgctttcg ctttttcgat atcgccagct ttaatcgcgt cggtaaaggc
     4861 tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca tacgctttat atgcagtaat
     4921 tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg cctcaccttt
     4981 gacgatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt attcgccagg
     5041 ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc gctcttccac
     5101 caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt tctgaataat
     5161 gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct tatccgtcac
     5221 ggtcactttg acctgcggca catcagcggc gttagccata aaagcagaag aaaacagcgc
     5281 agccacgctc aactgcaatg cgttacggca gaagttaatg gtcatgacga gactatccct
     5341 ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg agcgagacgc
     5401 tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga gataaataaa
     5461 ccagacggcg acttcgctga cgctcggcgc ttcctgatag ccaaaaatcc cttccatcag
     5521 cgtgccaaac agcgagtgag ttgagagcac cgcactcata tcgaaggcga tttcctgaaa
     5581 gtggttccac aatccggctt catgaaatgc gcgaatggca ccagctgcca gccctgcggc
     5641 gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga ggcgaatacc
     5701 gccccagtag agcagaaagc cgagcaccac ggctgtagca agaccgagca ttgcacccag
     5761 cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga ccgactccag
     5821 cccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat gatgatttcc
     5881 acgctacaat gcgctatcga ctgcctgttc cagttgcact tacgttgcgc gacactttgc
     5941 gcatccagaa aaccatccag gtaaggatca ccacggcgat caccgccacg ataccttcaa
     6001 acagttcctg ttctttttgc ggaaattcgc cggtggtttc gttaatgaag atccccaggc
     6061 ccaggcacaa cgcagcggca agcaacacgc caatccacat cacaccaatc catcggcctc
     6121 gctgggtacg cttaagatag ctggcaatca aactgacaat cagcgcggct tcaagtcctt
     6181 cgcgcaacat aatgagaaac ggaacaaaca tgccacccac ccttaaacgt ttttctgagt
     6241 caatctatgc aaagaaaagt aaatcgcagt gatagcgatt atcattacgg taaagaaaaa
     6301 tacaagcgga atgtttataa aatgatgttg gaatgtaaac aaagagaggt aatgccaggg
     6361 gatataaacg attattccag ggtagaaaga tgggattgtt tgcaaagaac catgtgttct
     6421 tgccggatgc cgcgtaaacg ccatatccag ccgacaaaac tgtccagact caatgcatca
     6481 gagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
     6541 caaatctaac tttttgcccg gaacataata acagctcggg tgttggtggc gtcaaaatcg
     6601 ctgaagcgtt ccccgaaggc cgggacagcc cacatggatg tgggctgagg gcgcgtctta
     6661 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagctgaag gtaccgcgca
     6721 gcggcgattt tgttcgccag agcccggggg tgcacagggg gcggcggcga ttggccgccc
     6781 cctgcgcgct ccctgcacca ttcaatttat atggcgaggc acattaaggg agcgcaaccc
     6841 gctgctgaag ccaaacaaaa ccgccgcagg ctaagtccct tagctttcct gcaaccgtga
     6901 cggcggagcc gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca tcgccgctga
     6961 cggcgcttta cccagcaaac taaacactac aatcccgata ctgccgaaga tgaagcccgg
     7021 aataatttcg tacagtccca gccagccgaa ctgcttccag acgataaccg tcagcgcacc
     7081 gatgatcatc cccgccagtg caccgttacg cgtcatgcgt gaccacatca ccgagaacag
     7141 caccaccgga ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta agcccagcac
     7201 gcggttttcc gggtttgccg ccagcgcaat cgccaccagc gccaccacca gcaccatcac
     7261 acgccctacc cacaccagct ctttctggct ggcatgttta cgcagaaacg ctttgtataa
     7321 atcttcggta atcgcactgg agcacaccag cagttggcaa cttaaggttg acattaccgc
     7381 cgccagaatt gccgacagca gaatcccggc aatccacggg ttaaacagaa tttgcgccag
     7441 ttcgataaac acgcgctcgg cgttctggtt taccgcgcct gctaccgccg ggtgctcatt
     7501 aaagtaagca atcccaaaga agccgacagc caccgcccct gccaggcaga ggatcatcca
     7561 ggtcatactg atacgacgcg catggacaat gctgtggtga gaatccgccg ccataaaacg
     7621 cgccagaatg tgcggctgcc cgaagtaacc cagcccccaa cccatcagtg agataatggc
     7681 aacaaagttc agacctttga gcatatcaac gttttcgatg ctcttttgtt tgatcacttc
     7741 cagcgagtca ccaaagccac cgacactgat aatgacgata accggtgtca ggatcagggc
     7801 aaaaatcatc aggctggcct gtacagtgtc agtccagctc accgcgagga aaccgccaat
     7861 aaaggtgtaa aggatcgtcg ccgcagctcc cgcccacaga gccgtttcgt agctcatgcc
     7921 aaaggtactt tcaaacagac gcgcgcctgc cacaatgccc gaagcgcaat aaatggtgaa
     7981 gaacagcaaa ataaccagcg cggagataat gcgcaaaatg cggcttttat cttcaaaacg
     8041 cccggtgaaa taatccggca atgttaaggc gttattattg tattcggtat gcacacgcaa
     8101 ccgcccggcc accagcttcc agttgatcca cgcgcctaat gtcaggccaa tggcgatcca
     8161 gctttcggaa atcccggaaa gaaaaaccgc gccaggcaac cccattaaca gccagccgct
     8221 catatccgac gcacccgccg ataatgccgt cacaaatggc ccaagactac gaccgcccag
     8281 aatatagtcg tcaaagtttt tcgttgatcg ccaggcgata aacccaatca atatcatgcc
     8341 aaagatatag acacaaaatg tcaccaacat cggtgtgcta atagccatct aaagtctcca
     8401 aaaaattatt atcggcaatg tcgaaacttg ccgttatatc tgcaaccgga acggggtaac
     8461 agagtttatt ttttaccagg gcgaccgtat cctgccggaa gcgctggtta ttcacaatcg
     8521 atttaacaca ccatttacat taaattttag tgctcagcga cactattttt catcaggttg
     8581 cactctctca cattttttgc ggttgcacct ttcaaaaatg ttaactgccg cagagaaaaa
     8641 gtctgagtta tttttttaat ccctgtcata tcaatttctt ttattaacat ttcattcatt
     8701 tttaagcttg ctacgcaggt cacatttaac atggttgcac aaagttgcaa catcatggat
     8761 atttcacgat aacgttaagt tgcacctttc agaacaacag gagtaatggc atgggaacca
     8821 ccaccatggg ggttaagctg gacgacgcga cgcgtgagcg tattaagtct gccgcgacac
     8881 gtatcgatcg cacgccacac tggttaatta agcaggcgat tttttcttat ctcgaacaac
     8941 tggaaaacag cgatactctg ccagagctac ctgcgctgct ttctggtgct gccaatgaga
     9001 gcgatgaagc accgactccg gcagaggaac cacaccagcc attcctcgac tttgccgagc
     9061 aaattttgcc ccagtcggtt tcccgcgccg cgatcaccgc ggcctatcgt cgcccggaaa
     9121 ccgaagcggt ttctatgctg ctggaacaag cccgcctgcc gcagccagtt gctgaacagg
     9181 cgcataaact ggcatatcaa ctggccgata aactgcgtaa tcaaaaaaat gccagtggtc
     9241 gcgcaggtat ggtccaaggg ttattgcagg agttttcgct gtcatcgcag gaaggcgtgg
     9301 cgctgatgtg tctggcggaa gcgttgttgc gtattcccga caaagccacc cgcgacgcgt
     9361 taattcgcga caaaatcagc aactgtaact ggcagtcaca cattggtcgt agcccgtcac
     9421 tgtttgttaa tgccgctacc tgggggctgt tgtttactgg caaactggtt tccacccata
     9481 acgaagccag cctctcccgc tcgctgaacc gcattatcgg taaaagcggt gaaccgctga
     9541 tccgcaaagg tgtggatatg gcgatgcgcc tgatgggcga gcagttcgtc acgggtgaaa
     9601 ccatcgcgga agcgttagcc aatgcccgca agctggaaga gaaaggtttc cgttactctt
     9661 acgatatgct gggcgaagct gcactgactg ccgcagacgc acaggcgtat atggtttcct
     9721 atcagcaggc gattcacgcc attggtaaag cgtctaacgg tcgtggcatc tatgaaggtc
     9781 cgggcatttc aatcaagctg tcggcgctgc atccgcgcta cagccgcgcc cagtatgacc
     9841 gggtaatgga agagctttac ccgcgtctga aatcactcac cctgctggcg cgtcagtatg
     9901 atattggtat taacatcgat gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc
     9961 tggaaaatct ctgtttcgag ccggaactgg caggctggaa cggcatcggt tttgttattc
    10021 aggcttatca aaaacgctgc ccgttggtga tcgattacct ggttgatctc gccacccgca
    10081 gccgtcgccg tctgatgatt cgcctggtga aaggcgcgta ctgggatagt gaaatcaagc
    10141 gtgcgcagat ggacggcctt gaaggctatc cggtttatac ccgcaaggtg tataccgacg
    10201 tttcttatct cgcctgtgcg aaaaagctgc tggcggtgcc gaatctaatc tacccgcagt
    10261 tcgcgacgca caacgcccat acgctggcag cgatttatca actggcaggt cagaactact
    10321 atccgggtca gtacgagttc cagtgcctgc atggtatggg cgagccactg tacgagcagg
    10381 tcacagggaa agttgccgac ggtaaactta accgaccgtg tcgtatttat gccccggttg
    10441 gcacatatga aacgctgctg gcgtatctgg tgcgtcgcct gctggaaaac ggtgctaaca
    10501 cctcgtttgt taaccgtatt gccgacacct ctttgccact ggatgaactg gtcgccgatc
    10561 cggtcactgc tgtagaaaaa ctggcgcaac aggaagggca aactggatta ccgcatccga
    10621 aaattcccct gccgcgcgat ctttacggtc acgggcgcga caactcggca gggctggatc
    10681 tcgctaacga acaccgtctg gcctcgctct cctctgccct gctcaatagt gcactgcaaa
    10741 aatggcaggc cttgccaatg ctggaacaac cggtagcggc aggtgagatg tcgcccgtta
    10801 ttaaccctgc ggaaccgaaa gatattgtgg gctttgtgcg tgaagccacg ccgcgtgaag
    10861 tagaacaggc gctggaaagt gcggttaata acgcgccaat ctggtttgcc acgcctccgg
    10921 ctgaacgcgc ggcgattttg catcgcgctg ccgtgctaat ggaaagccag atgcagcaac
    10981 tgattggtat tctggtgcgt gaagccggaa aaaccttcag taacgctatt gccgaagtgc
    11041 gcgaagcggt cgattttctc cactactacg ccggacaggt gcgggatgat ttcgctaacg
    11101 aaacccaccg tccattaggg cctgtggtgt gtatcagtcc gtggaacttc ccgctggcta
    11161 ttttcaccgg gcagatcgct gccgcactgg cggcaggtaa cagcgtgctg gcaaaaccgg
    11221 cagaacaaac gccgctgatt gccgcgcaag ggatcgccat tttgctggaa gcgggtgtac
    11281 cccctggcgt ggtgcaattg ctgccaggtc agggggaaac cgtgggcgcg caactgacgg
    11341 gtgatgatcg cgtgcgcggg gtgatgttta ccggttcaac cgaagtcgct acgttactgc
    11401 agtgcaatat cgccagccgc ctggacgctc agggtcgccc tattccgctc atcgctgaaa
    11461 ccggcggcat gaacgcgatg attgtcgatt cttcagcact gaccgaacaa gtcgtcgtgg
    11521 atgtgctggc ctcggcgttc gacagtgcgg gtcagcgttg ttcggcactg cgcgtgctgt
    11581 gcctgcaaga tgagattgcc gaccacacgt tgaaaatgct gcgcggtgca atggccgaat
    11641 gccggatggg taatccgggt cgcctgacca ccgatatcgg tccggtgatt gatagcgaag
    11701 cgaaagccaa tattgagcgc catattcaga ccatgcgtag caaaggccgt ccggtgttcc
    11761 aggcggtgcg ggaaaacagc gaagatgccc gtgaatggca aagcggcacc tttgtcgccc
    11821 cgacgctgat cgaactggat gactttgccg aattgcacaa agaggtcttt ggtccggtgc
    11881 tgcatgtggt gcgttacaac cgtaaccagc taccagagct gatcgagcag attaacgctt
    11941 ccggctatgg tctgacgctt ggtgtccata cgcgtattga tgaaaccatc gcccaggtca
    12001 ctggctcggc ccatgttggt aacctgtatg ttaaccgtaa tatggtgggc gcagtggttg
    12061 gtgtgcagcc gttcggcggc gaagggttgt ccggtaccgg gccgaaagca ggcggtccgc
    12121 tctatctcta ccgtctgctg gcgaatcgcc cggaaagtgc gctggcagtg acgctcgcgc
    12181 gtcaggatgc agagtatccg ctcgatgcac agttgaaagc cgcattgact cagccgctaa
    12241 atgcactgcg ggaatgggca gcaaatcgtc cagaattgca ggcgttatgt acgcaatatg
    12301 gcgagctggc gcaggcagga atacaacgat tgctgccggg gccgacgggt gaacgcaaca
    12361 cctggacgct gctgccgcgt gagcgcgtgt tgtgtattgc cgatgatgag caggatgcgc
    12421 tgactcagct cgccgccgtg ctggcggtgg gcagccaggt actgtggccg gatgacgcgc
    12481 tgcatcgtca gctagtaaag gcattgccat cggcagtcag cgaacgtatt caactggcga
    12541 aagcggaaaa tataaccgct caaccctttg atgcggtgat cttccacggt gattcggatc
    12601 agcttcgtgc attgtgtgaa gccgttgctg cgcgggatgg agcaattgtt tcggtgcagg
    12661 gttttgcccg tggcgaaagc aatatccttc tggaacggct gtatatcgaa cgttcgctga
    12721 gtgtgaatac cgctgccgct ggcggtaacg ccagtttaat gacgatcggt taaacaggta
    12781 gccggaggat gttacaccct cctccggcat ctttaacgtg gtcgaatccc ctcaataata
    12841 atccgctgca cgttttcaac cgtttgattg aaaaatacct catcgcgcaa cgttgcgcct
    12901 gtcaccgcct ccacctgagg ggcgaaatcg gcgtaatgtt gagtggaagc ccaaatcata
    12961 aaaatcaaat gctgcggatc aatcggcgcg agtttgccgc ttttgaccca accggcaatc
    13021 agcgccgatt tctcatcaat taatgccttc aaatcgcccg tcagttcatc cattaacagc
    13081 ggcgcgcctg ccagcatctc catacagaac aggcgcgaag cctgcggata atcgcgtgag
    13141 acttccagct tcagacggat gtactctttg atcgccgcca gcggggcgaa atcttcacga
    13201 aacgctttta acggtgccag ccagatatcg agaatctgcc gcagcacggc aatatacagc
    13261 gcctcttttg acggaaagta atacagcaga ttggtttttg aaacacccgc caactctgcg
    13321 atctgctcca gccttgtgcc gtgaaaaccg aattgtgaaa aagtgtccag tgctgcgcta
    13381 agaatcgctt ttttcttcgc gcttactgcg cgcgaacgtt tacccgttgt tttcactgcg
    13441 ccttgcgtca tgcgctctcc cctcttgtag attggcaaca ggatagcaaa acccccgccc
    13501 ggcctcgacc ttctgcactc tcatcgcgca cggatgcatg ttttatgtgc aactgttttg
    13561 accgtttagt ccacttttta ccagatattt tagataacac aatgctaaca cattaataac
    13621 attcactttt taaaaactgg catccgcttt gcaaacaagc caatacgcgg ctgaaaagga
    13681 tatgcaggat gcagcgcccc gtctgacttt cactttacga gacgaagaga ggttgatgat
    13741 gaaaattggc gtattcgtac ctattggcaa caacggctgg ctcatttcga cccacgcgcc
    13801 gcagtacatg ccgacctttg aactgaataa agccattgtg caaaaagcgg agcattacca
    13861 tttcgatttc gccct
//
LOCUS       DADLTD010000107        13621 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLTD010000107 DADLTD010000000
VERSION     DADLTD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.107"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..1862)
                     /locus_tag="JN701_002497"
                     /pseudo
     CDS             complement(1..1862)
                     /locus_tag="JN701_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2260..2958
                     /locus_tag="JN701_002498"
     CDS             2260..2958
                     /locus_tag="JN701_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY7364917.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3064..3139
                     /locus_tag="JN701_002499"
     tRNA            3064..3139
                     /locus_tag="JN701_002499"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3097..3099,aa:Phe,seq:gaa)
     gene            complement(3288..3824)
                     /locus_tag="JN701_002500"
     CDS             complement(3288..3824)
                     /locus_tag="JN701_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAY7364918.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRITERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="JN701_002501"
     CDS             complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="JN701_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HAY7364919.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="JN701_002502"
     CDS             complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="JN701_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HAY7364920.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="JN701_002503"
     CDS             complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="JN701_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HAY7364921.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="JN701_002504"
     CDS             complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="JN701_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HAY7364922.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVFEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="JN701_002505"
     CDS             complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="JN701_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HAY7364923.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="JN701_002506"
     CDS             complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="JN701_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HAY7364924.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7984..9208)
                     /gene="gspF"
                     /locus_tag="JN701_002507"
                     /pseudo
     CDS             complement(7984..9208)
                     /gene="gspF"
                     /locus_tag="JN701_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
     gene            complement(9208..10700)
                     /gene="gspE"
                     /locus_tag="JN701_002508"
                     /pseudo
     CDS             complement(9208..10700)
                     /gene="gspE"
                     /locus_tag="JN701_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10700..12760)
                     /gene="gspD"
                     /locus_tag="JN701_002509"
                     /pseudo
     CDS             complement(10700..12760)
                     /gene="gspD"
                     /locus_tag="JN701_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12790..13607)
                     /gene="gspC"
                     /locus_tag="JN701_002510"
                     /pseudo
     CDS             complement(12790..13607)
                     /gene="gspC"
                     /locus_tag="JN701_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
BASE COUNT         3051 a         3928 c         3513 g         3129 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcaaagtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaactgcgc caacatctct accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acactgatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc cccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cggcgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatctttc gccaataagc
     1381 agatcgccca attttacgtc agcgttacac atatcggcgc gaaagacgtt ctcaccaaag
     1441 aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa acgcaccatg ttgatgtcca
     1501 gggcaagcaa aggtgctgtt gcccatctca acgtactgcg tcagcgtgtc ataaaacggt
     1561 ggcagcaaat tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg
     1621 ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa
     1681 aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc
     1741 gcagcggtaa tgacgactgc cacgacgtcc gtaaaatcag tatctcccaa cgccaccacg
     1801 cgacgatgag aagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc
     1861 ataagcgcaa acccgtttcg gggaagtaag aataccggac aaggtggaaa accctgcccc
     1921 atgagatatg ggtcaaactg gtcaccagct ccgaccgcca gacatcagta aaagcagaaa
     1981 cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta acttcaccgc atgagcagta
     2041 acatagaaag ggaaacgttt cgcgcgaaac ggcgataagc gagagaatgt aaggaggtgg
     2101 cgtgcatcgg gcaaactccg tacagagagg agaaaattcg cgcaatcatg gcacctttcg
     2161 ctaaagcgtg caagccagga ctttgcgacc agacaagcca tcaacaaggc aaaaagttca
     2221 taataagaaa atcaaacaat acagttaaca ggaactcttg tggtcatcgg cccttttatc
     2281 aacgctagtg cagtcttact gggtggcgtt ctcggcgcac tgctcagcca acgcttaccg
     2341 gaacgtatcc gcatctccat gacatctatt tttggtctgg catcgctggg gattggtatt
     2401 ttactggttg tgaaatgcgc caaccttcca gcgatggttt tagctacgct acttggcgct
     2461 ctaattggcg aaatttgttt gctggagaaa ggtgtcaata cagcagtcgc caaagcacaa
     2521 aatctgtttc gtcactcacg taagaagcca acgcatgaat cttttattca gaattatgtc
     2581 gcgattattg tcctgttttg cgccagcggc accgggatct tcggggcgat gaacgaaggg
     2641 atgaccggcg atccgagtat tttaatcgcc aagtcatttc ttgatttctt tacggcgatg
     2701 atcttcgcct gctcgctggg tattgcggtg tcggtaatta gtatcccatt actgatcatc
     2761 cagttaacgc tggcgtgggc tgccgcgctg atattaccgc tgaccacacc gtcgatgatg
     2821 gcagacttca gcgccgtagg cggtttattg ctgctggcaa ccggattacg catctgtggc
     2881 attaaaatgt tcccggtggt caacatgctt ccagcactcc ttctggcaat gccgctttcc
     2941 gccgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag attgtgcagt
     3001 ctgcagtaaa ttgaagaaat ttgattgacg agacgaggcg aatcaggttt aatgcgcccc
     3061 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
     3121 attccgagtc cgggcaccac taattcttaa gaacccgccc acaaggcggg tttttgcttt
     3181 tggatctgac aataaccttc acgaaaaaag tttgcttata aagtctgggg gaattactct
     3241 cgccacgtta acgagagtaa ttttattgat actaatctcc tgatacttta cccccgtcca
     3301 aactccagcc gctggacatt caccatccca gtcttctcag cagcactggc atcaatttgt
     3361 gtcacccgca gcgcatactt ttcatccagc gcgtttaacc agttcagcag gtcattaaac
     3421 accaccggat ctatccagac ctgaatattc tccccacgct cggttatccg cttgatgact
     3481 accgagtgcg cggaagcact gtcactgata acccgcgata cctgcgcagg cgttgttgtg
     3541 gcggattttc gcgccgcaat aatatccggc gcggcgctcc tcagtctggt gttcatcgcc
     3601 accagttgct gcaacattgt ctcctgttgt actatccgtt cgctcaacgg ctgccagatg
     3661 aggacgtaat atccgacgct aaacaggaac accaccgctg ccagtaacat gcccttttca
     3721 cgcggagaac gccccgccag gtgttgtgcc agccagtgtt cgccacggct taactggcgt
     3781 tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt tcctccgcaa
     3841 cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt gcacaacata
     3901 atcggttgcc agtgcactac gcagtttgtc gaaactggca aagttcgcgg cccgtagctg
     3961 gaggtgaagt atctggcgtt tttgatcaaa ggtaaaacca cgcatttcga tgtcggaaag
     4021 tgacgctgat ttcagggtgc tggctatcgc tgacaactcg gcgagcagcc gggtatcgtc
     4081 ggcctgtggg cgatattttt tcagcgccat cgtcacctga gagcgtaaat tcacaatccg
     4141 cttctgctcc gggaacaacg ttaagaactg ttcctccgcc tgggtgcggc tttgcgccac
     4201 ctgttcgctg acgctccata acgtcacgcc ccgctccact gccagcgcaa ctagaatcag
     4261 caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc tgacgcgagg
     4321 ctgccacggc cctgtcagca ggttcccttc cggttcgcca taagtggtaa cggcgggcag
     4381 agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat gctgatagct tttccggtgc
     4441 aatgccgact acggttagcg aaagcggtaa atcctgctca ttgagctggg cgcggaacat
     4501 gaccggagcc agcgcccgcc cggcgctcca tccccggcat tcatcaatgc ggcagataac
     4561 ccgttgcgca tcgccagcca taaccccaca aggaatggac atccagtctg gcgcgacggt
     4621 agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg ttgcgcatat gctgttggtg
     4681 aatcactgct acggttgcca gttgctggtc gatattcaac ggggcgaaat gcagttcatc
     4741 gatatcctgg ttcagctctt cttccagtaa ggcgggcaga atggtcggta tctgcttacg
     4801 ggcgacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa tgtagagtcg
     4861 aatcgcatcg gtttgcagcc attgctggag ctgttcgtta gcaacgtcag accagatacc
     4921 gtgctccaca tcgggtgtac ggcgctgcca atggatggga tcagcggcgc aaagcgggaa
     4981 aaaaatctca aggatggaac tcactcactt tctcctgtct gatgccagag aaccgaaaag
     5041 tgttgtgggc ccatgcggac aattaacgag ttcatcgtca gttcaatctc attcacggtg
     5101 atatctgaac gcagccagaa gtaattgctg tccacgctca ggacggtttt tagctgtttt
     5161 ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa gaaactgatc gacatcttcc
     5221 cagcccttcg ccggacgttg ttgtaataac gcccgcgcct gaacggggct taaccacggg
     5281 tcaaacagcg cctcaagaat cacactttgc gtgacgtcta aagtattgat gttgatttgc
     5341 tggcggatca tcggcagcgc acagaccagc ggtttcagtt tttgataaag cccggcgtcc
     5401 attccctgta ccacgcgcat ctcactgata tcagccagcg gttgattggc ggcgtagaac
     5461 ggcaccgaac gggcgagata ctcgctgtct tcacggccca gacgtgtctg cacgctgcgg
     5521 tcctcgtcaa taaactccca caggctttcg gctatcagtt cggcccgata agcaggcaca
     5581 tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa gcggacgcga cgccgttgtc
     5641 ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt cacgcagggt gaccgcaatt
     5701 tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc acggctgtgc cagatgcacg
     5761 cgcttttcat ttttcaggct cagactgagc gcgctcagcg ccaggctttc cgcactggcg
     5821 ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc
     5881 attcgcccgg aaagcgtgat ggttaccagc atcataaccg ccagcaatac cagcaccacg
     5941 accagtgcca ttccgcgttt tggtggtgag gtgatcatga ttattgcggc ccgcgtaaca
     6001 accagatgcg ttcaatctcg ccccattgcg gcgaatgcag ggtcatgcgt accgccacag
     6061 ggatcgcctg tactgacgac cagctctcct gccagcgcgt gccgtcgtag aactgcaaac
     6121 ggagcgaatc ggccggaatc aacttttgca ttgtcggctt cacgctgcct gcggcatcgg
     6181 tcagcggcca ggctaaccgt tcgagataac cgtcatgaat gcggtaaccg acggtgagca
     6241 gattgctgcg cggcagacgc atcaacgggt taaccacacc gccgcgcaca aaacgcattc
     6301 cttcactctc agacgccagc acgccagcgc cagccagtaa agctggttca cgctggccct
     6361 gatcgcctct taccggacgc ggcatcattt gggtcagatc gtgggtcaga aaactcatcg
     6421 tttgctgcat gaggtttagt ttttgatcgt gatcggcgac ggcgctattc acgcgcgtga
     6481 cgccgttagt cacctgctgc gccatcagtg ccagcgaggc aaaaatggca attgccacca
     6541 acatttccag taacgtgaaa ccagcgcgag tccttctcac tgttggcctc ccacggcgct
     6601 aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc tgacttcaat
     6661 atccaccgcc tggagcagcg cgttgcctgt attcaatggg attgttcgcc agtaccagcg
     6721 gcttcctgcc atatcgctct cgccctgatg cctgaccgcc cgctgctcac gggtcatcag
     6781 gtagagcagc gcagtctggt tttcagctac ccagcccgct accgtttttt cctcaaaaac
     6841 gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc gccgtggcag ccagcgcaaa
     6901 aatcgccagc acgagcatca cttccagcaa ggtaaatccg cgcttcatgg catcctctca
     6961 tcacattgat tgagggataa cgcgccatcg tgtgccagct ttaccgctca acatgcgttt
     7021 tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg
     7081 atctgcggcg tcttcttttt cgcctctttt tgcaactcca gttcaatatc gtgcagcgtc
     7141 aggcgacgac gttgcagctc cagcgcatat tccttctgcc agatatcact tccgggttgt
     7201 aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt cgcagaaacg
     7261 ggcaaccact gcccctgacg acgctgcata aactgatagc caggcgggtc gattagcaca
     7321 ccgagtgtct gtccttcaat cactgccctg tccttaaact gcgcaaagcg agtcagaaaa
     7381 tcctgtgccg cttttttcgc gggcggttca gaatcggtcg caaacgtctg taccacgccc
     7441 gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt gaatccgcgt
     7501 tcaggcactg cttactgaaa ctcctgcaaa ttccagttac cgatatctgc gccagcgcct
     7561 tccccgtttt cctgaccatc cgcccccagg gtataaacat caaacagtcc tttttcaccc
     7621 gggctgagat actgataatc attgccccac ggatcctttg gcagtcgttt gatgtatcca
     7681 ccagtacggt agttgcggga gtccgccata ttggctggct gctggatcag cgcttccagc
     7741 ccctgttctg tggtcggata acgcccgtta tccagtcggt acatatctag tgcgttttcc
     7801 agcgccacga tatcgctgat ggctttttgc cgattggctt tctctttgtt gcccaacagg
     7861 ttaggcacca ccagacttgc caggacgcca agaataacaa tcaccaccat cacttccagc
     7921 agggtaaaac ctgcccgtgg tttgtgtgtg cgggataacg aattcatgtg ataactccgt
     7981 aaattacatt ccaaccatat tattcagttg cagcatcggc tcgaggatgg cgatgacaat
     8041 aaacagcacc acgcccgcca tcatcaccac cagcgcaggc tcaaacagcc ccaacgccag
     8101 ccccacctgg gtatcaaatt cccgttcctg gttaacagcg gcctgctcaa gcatggtttc
     8161 cagttcgccg ctctgttcgc cggaagcgat aatgtacagc atcatcggcg ggaacaggcg
     8221 caactccgcc agcgcggcgc gcagactgct tccttcgcgg acgcgatctg ccgccagcag
     8281 cagttgttgt tcgacatagc gatttgccga cacggcggca gcggtctgaa tgccttccag
     8341 tagcggaaca ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg ccgtattcag
     8401 cccgcgcgca acacggcccg tcacgggcag acgcagcaag gttttatccc agcgcaggcg
     8461 catagtagga tttttaagta gccattgccc cagcaccaga agcgccagca atcccgccag
     8521 ccagtaaacg ccgctggcct gtaacgcatc actcatagcg ataagcgcac gggtggagga
     8581 gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt ttcggcacca ctgccgtcag
     8641 caaaatagtg accacgcccg ttgccaccac cagcataacc agcggataga gcatggcctg
     8701 caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa tctgccaggc gattgagcac
     8761 cacgtcgaga tgcccggatt tttctccggc agcgaccatc gaacaaaaca gggaatcaaa
     8821 gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa tccggctgcg
     8881 cagcgccatt ccgaggctct tcacatgcag tttttcactt tgctcactga ccgcctgcaa
     8941 gcaggtttcc aacggcattg ctgcctgcac cagcgttgcc agttggcgcg tgaacagcgc
     9001 cagatctgcc gccgccacgc gacgatgtgc gtgccgccga cgctgcaaca ttccccctgc
     9061 cgatgcattc atcctggctt caatgtgcac ggggataagc tctttaccgc gcaacaactg
     9121 gcgggcatga cgcgcggaat ccgcctcaat catgcctttt ggttttgcga ccattacgct
     9181 ccagcgcctg atagtaaaac agtgccatta cgcctccatg gttacccgca gaacttcatc
     9241 gagagaggtt tctccggcga gcactttctc aatgccgttg ctgcggatac ccgcagagtg
     9301 ttgccggaca taacgttcca gctccagctc cccggcctga cggtggatca aatcacgcaa
     9361 tgtggcatcc accacgtcag ctcatggatg gcagtccgtc cgcggaaacc tttgtgatta
     9421 caggcggggc agccctgcgg atggtacaga gtgacggttc gcgcatcggt aatgcccagc
     9481 aggcgttttt cttcgtcagt ggcaggcgcg gcctgacggc agtcgggaca cagcgtgcgg
     9541 accagtcgct gcgccatcac gcccgtcaga ctggaagaga gcaggaaagg ctccacgccc
     9601 atatcctgca aacgcgtgat cgcccccacc gccgtgttgg tatgcagcgt ggaaaggacc
     9661 aggtgtccgg taagcgatgc ctgaacagcg atttctgcgg tttcggtatc gcggatttca
     9721 ccgaccatca ccacatccgg gtcctgacgc aaaatcgcgc gcaggccacg ggcgaaggtc
     9781 atgccgacgc gggtgttaac ctgtgtctga ccgatccctt caatcatgta ttcgatgggg
     9841 tcttcaaccg tgagaatgtt acgcgaatgg ttgttcagct cctgcaatcc ggcgtacagc
     9901 gtggtgcttt tgccggaacc cgtcggcccc gtcaccagaa agatgccgtg cggtttgtgt
     9961 aacagctggc gcaactgcac ggtcagttgc tggctaagcc ccagacgctc cagcgtcagg
    10021 cgggcctggt ttttgtccag cagtcgcagc accacgcgct cgccccaggc agaaggcatg
    10081 gtggagacac gcacgtcaat cgcccggccg cccagcagca gcgcaatacg tccatcctgc
    10141 ggtacgcgct tttcggcgat atccagccgt gccatcacct tgatacgcga taccagcagc
    10201 gaggccagtt tgcgccccgg gcgcagcatt tcatgtaatg tgccgtcaac gcgaaaacgg
    10261 atcaccagac tcttttcaaa cgtctcgatg tggatatccg aagcgccttc tttaatcgcc
    10321 tctgccagca tggcgttgat cagtttgatg atcggcgcat cgtcgtcact ttccagcaga
    10381 tcttccgttt ctggcagttc ttccgcgaga gtaaaaaagt cctcagcaga accgagatct
    10441 tccatcagct ggcgagcctc ggaagagtcc cgctgccaga ccgcattcag ccgctgttca
    10501 aattcggcct cgtcgatttg ccgcagcgta aagggcgcgt tcagcccccg ctgcagctcc
    10561 tgcaaaacag agagcgatag cggatgaacg tggaggatct ccagcgacgc ttcgcaccat
    10621 gccaccaggc taaaccgacg gctgaaactg tagggcagac gcacggtctt ggcggtggtt
    10681 tcctgtgcta caggcaccat taacgcgttc tcccggcatt gaggaacgcg cgaacttccg
    10741 gcggtaaggc ctggttttgc gctggcagta ccggctgtgc ggtgtgcggc atcaggctta
    10801 aaccttgctc atcacgatag atctgttcgg cgcgcatata gttatatttg cgctgcgaca
    10861 cgccgtctgc cgccataccg tcatgcagaa tggtcgggcg gataaacacc atcaggttac
    10921 gtttttcttt tttatccgcc gtcgatttaa acaggttacc aatcaatggg atatcgccca
    10981 gcagaggcac tttcgccacg ctttctcccg cctgatcgtc catcagacca ccaagcacaa
    11041 tcagctcacc atcgttagcc agcacggtgg ttttcagttt gcgctcacca aacaccacgt
    11101 cgaggctggt ctgtccttcc accttcgaga cttcctgctc aatcaccatc tgtaccgcgt
    11161 taccttcgtt aatcagcggc gtgactttca gcatgatgcc gacttttttc ctttctactg
    11221 tattgaaagg attgctgtta ttggagccaa cggtagagcc ggttaatacc ggaacgtcct
    11281 agcccaccat gaagaaggct tcctggttgt ccagcgtagt gatgctcggc gtggagagaa
    11341 cgttcgagct ggagtcgttt ttaaccgcct gcaccagtgc catccagtcg cctttcacca
    11401 cgccaaccgc cgtaccgcta aatccagaaa gaagctgggc gagtgtggag agatcgccgt
    11461 tggtatccgg attaatggtg gtagcgccgt tttcactgat caccgtcgag cctttctgcg
    11521 gtttcgcctg agaaatggct gcccccagcg taccgatagg gatctgcgta ccattagcaa
    11581 actgcattaa tccggcatct ttcgacgccc actgcacgcc gaagttgata ttgctacctt
    11641 cggcaacttc cacgatcaac gcctcgacgt gcacctgagc acggcgaata tccagttgtt
    11701 caattacgct ttgcagcgac tgcatgatgt cctgcggcgc ggttacaatc agggcattac
    11761 tgtgtttgct ggcggcgatg gatacaacct cacgcccgct accgaccgtg ccttccgcct
    11821 cttctttagc cgccgtgagc gtgccgctga cctgtttcag cacatcgacc agatcttcgg
    11881 ctttgctgta tttgagatag aaaacctggc tgttgccgct gcgttccatt tctgagtcca
    11941 gccgacggat caggcggcgc attttgtccc gcgtggccgg gtcaccactg acaatcacac
    12001 tgttggtgcg ttcgtcggcg acaatttgag atttcagcgt cgcaggctgg ttctcgccgc
    12061 tgtttttcgt caggctttcc agcacgcggg cgatttccga agccgaggcg ttatccagcg
    12121 ggatcacctc ttcggtgcga ttacccgcgt gatccacgcg ctggatcact tccgtcaggc
    12181 gctccacaac ggaggcgcgt ccggtgagca taatcacgtt ggagggatcg taattaacaa
    12241 cgttgcctga gcctgcgctg tcgatcatct ggcgcagaat cggtgccagc tcgcgtaccg
    12301 acacattacg taccggcact actttggtga ccatttcatc gcccgcgtag ttgtcgctgc
    12361 cttcaccgac cagcggcagc ggctcgactt tcgcagcgct ggatttcacc accttcagca
    12421 cgtcgttttc catcggtacg acggcatacc cctgtgcttc cagcaggtta aggaatagct
    12481 ggtaatactg gcgttcattg agcggggtca tggtgcgaat actcactttc ccctgtacgc
    12541 ccggccccat aatgatggtt ttattaaggt tagcgccgac ggtttcgatg aacgatttca
    12601 ggtcggtatc tttaaaatta gcggtgaaag tggcttcttc cgcccagacc ggtgaactgc
    12661 ataatgccgc tgccagcacc agcggcagta aacgcttttt tgttttgagg ccagttgtct
    12721 tcttacgcca gaccgacaac gtaatatcac gccaaaacac gatgaatgat tctcctggat
    12781 taaatgcggt tagcgcagcg cgatggaaat gtcgtggcgc gcacccttgc gttgaatgga
    12841 atccattgaa ggtaactgcc gcatcagagc aatcattgct cgtggatcag tgaaatcctg
    12901 ctgatttagc gcaatggcga tatcgccttc cttgaaaccg ctggcatcga acagagaacg
    12961 atctgccccc ggcttcactg cataaccgac aattccctcc ttacgcacag gcgtaagctg
    13021 gatatagtta aaaattttct gcggatcttt cgccagtgcc tgacgcacgg cagtcggtat
    13081 ctcaactggc gcactgacag caggttcagc aacagcttgc tttgcttcgt cactgacagc
    13141 ttttttgttg gtcgcggcaa cggtggaacg ctcctcttca gccaggctca ggcgctctat
    13201 ttttccctga tagcgcagca tcacatggtc gcgattgatt tcctcaatca ccgcgttgtg
    13261 cgagccaagc gtttcaccct gcaaatagac ctgctgttta ccgccttctt caataaccgc
    13321 gccgggtctg gcaccaaagg cgatcccgcg cgcaccacat taagacgcgt ttctgccaca
    13381 ggcacaggtt cgggttgttt taccggcgtg gcgacgggct gatatttgcc aaaccagttt
    13441 tgctggctga ttaattgcac gtcattttta tcgaacgttt ttgcatccgc acggggcggt
    13501 ttattcgccg atggggaaac agccgtatat tccgcagaaa aggagaaata gcgccagagt
    13561 gaatgcgcca cttttgcaga aataataagc agcatcagcc agaacatccc gcgtacaatt
    13621 t
//
LOCUS       DADLTD010000108        13583 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLTD010000108 DADLTD010000000
VERSION     DADLTD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.108"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(241..1614)
                     /gene="cobA"
                     /locus_tag="JN701_002511"
     CDS             complement(241..1614)
                     /gene="cobA"
                     /locus_tag="JN701_002511"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7364925.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1633..2439)
                     /gene="nirC"
                     /locus_tag="JN701_002512"
     CDS             complement(1633..2439)
                     /gene="nirC"
                     /locus_tag="JN701_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY7364926.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2566..2892)
                     /gene="nirD"
                     /locus_tag="JN701_002513"
     CDS             complement(2566..2892)
                     /gene="nirD"
                     /locus_tag="JN701_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY7364927.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2889..5432)
                     /locus_tag="JN701_002514"
     CDS             complement(2889..5432)
                     /locus_tag="JN701_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY7364928.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5694..6875)
                     /gene="tsgA"
                     /locus_tag="JN701_002515"
     CDS             complement(5694..6875)
                     /gene="tsgA"
                     /locus_tag="JN701_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY7364929.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7146..7718
                     /gene="ppiA"
                     /locus_tag="JN701_002516"
                     /gene_synonym="rotA"
     CDS             7146..7718
                     /gene="ppiA"
                     /locus_tag="JN701_002516"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY7364930.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7823..7990
                     /locus_tag="JN701_002517"
     CDS             7823..7990
                     /locus_tag="JN701_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY7364931.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7980..8582
                     /locus_tag="JN701_002518"
     CDS             7980..8582
                     /locus_tag="JN701_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY7364932.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8614..9177
                     /gene="pabA"
                     /locus_tag="JN701_002519"
     CDS             8614..9177
                     /gene="pabA"
                     /locus_tag="JN701_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY7364933.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9262..10482
                     /gene="argD/dapC"
                     /locus_tag="JN701_002520"
     CDS             9262..10482
                     /gene="argD/dapC"
                     /locus_tag="JN701_002520"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY7364934.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10549..12639)
                     /locus_tag="JN701_002521"
     CDS             complement(10549..12639)
                     /locus_tag="JN701_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364935.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12690..13322)
                     /gene="crp"
                     /locus_tag="JN701_002522"
     CDS             complement(12690..13322)
                     /gene="crp"
                     /locus_tag="JN701_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY7364936.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3290 a         3495 c         3555 g         3243 t
ORIGIN      
        1 tccctatgta ttatatatta attaatattc attgattata tgaatataag cagactgctc
       61 gttaagaacg atacaggaat atttaattgg cgtcagtgag cgaatgactt aattttgtaa
      121 gtcgctgtaa cggtgaatat aacccattag cagataatat ttattaaggg gttatgtttt
      181 atttgctgaa tatataaaaa gagtcattaa gaaggccaga attctctggc ccgggttaaa
      241 ttaatggttg gagaaccagt tcagtttatc gcgcaggcca acaacccgac caataataat
      301 tagcgatgga ctgttcattt gctgagcaag ttcaccgagt tgcgtgagcg taccgtcaat
      361 cacgcgctgc gtgactgccg ttccgttttc gacaattgcc accggcattt cgccaggcat
      421 accgtgttca atcagctttt gctgaatagt cgcggcctga ttcaacccca tatagaacac
      481 cagcgtctgt ttttctgccg ccaggttttc ccagtccagc tcgccaccgg tttttaagtg
      541 tccggtaatt aagcgtacgc tctgggcata atcgcgatgc gtgagtggaa tacccgaata
      601 ggcagagcaa ccagaagctg cggtaatacc cggaaccacc gagaacggaa tacccgcgtt
      661 gcacagtgtt tccagctctt cgccaccacg gccaaaaata aacggatcgc cacctttcag
      721 ccgcaccacg cgtttgcctt tttgcgcttc ccgcagcagg atctggttaa tctcttcctg
      781 cggtacacag tggtatcccg cgcgtttgcc gacgaaaacg cgatcagcat cgcggcgtac
      841 cagattcata atatcgtcag aaaccagacg gtcgtagacc accacatctg cctgctgaat
      901 ttgttgcagt cctttcagcg tcagcagccc ggcatcgccc ggacctgcac caaccagcac
      961 cacttcaccg cgatggtcga gcggttcgtt gattaactgt tcggtcgttt cagtaatggc
     1021 tttctgatcg ttgtttgcca gcgactgcgc caggcggtcg ttaacgaaca atttctccca
     1081 gaaacggcga cgctcactca tcgtggtgaa ctgttgtttc actcgcccgc gtaattgccc
     1141 ggcatatttc gccacctggc ccagatgcaa cggcagcagt gattcgagtt tttcgcgcaa
     1201 caggcgcgcc agaaccggag aggtgccgcc agaggagacc gctaccatta gcggtgagcg
     1261 gtcaataatc gacggcataa taaagctggc ggctttcggc gcatcgacta cgttacagaa
     1321 gatgcgacga gcttcagcgg cttcgctgac gcgctggtta agcgtgtcat catccgtcgc
     1381 tgcaatcgcc agccagcagg tgtcgagaag gctttcatca aatggccctt cgacgagggt
     1441 taacatgcct gcatctgccc atgcggtgaa ctgtggaata aacgctaatg cattcaccgt
     1501 taagcgagcg cctgcgtcta acagcaacct tgctttgcgt tccgcgacat caccaccgcc
     1561 gacaatcaga cagtcgcgat cgcgtaattg gcaaaatata ggcaaatgat ccacgtaaaa
     1621 accccttagt aattaaccgg cagccgtttc agtttgatta aatttgtccg caaccggacg
     1681 attcgatttc ggcgtagcat accaataacc caatcccatg aatacggcac ctgataaagt
     1741 attacccagc gtcacccaca gcaggttatg accaataccc gccagcgtgt aggcttcgct
     1801 gtggttgccg aaccaggaga gcgcgaacag cgtcatgtta gcgatagagt gctcgtagcc
     1861 ggacgcgata aatgccagca gacaccacca gatagcgata aatttcgccg ccccttcagt
     1921 gcgcagcgcc atccagattg ccaggcaaac cagccagtta cacaatgcac ctttgaagaa
     1981 gagtaccatt gccggtgcag tggttttagc cagcgcgacg gagtgaacga tgctggtatc
     2041 taccggcagc aggctaccgc cgccccagct atagagcatg gcaacgaaga cggaaccgac
     2101 caggttaccc agccaggttt gcggcaggat tgcccacatt tgcccgtggc tgatgctgcc
     2161 cgcttttacc ccaaaggtga ggaacatggt gtgtccggtg aacagttcag aaccggcgat
     2221 aatcaccagc gttaaggcga taccaaaggt cgcgcccatc accagcggac gtacggacgg
     2281 gtcgagcaaa ttaccgagcg tgaaaatcaa gatgatccca agacccacat acgcgcccgc
     2341 catggcggag ctgacccaaa agccgagcgg gttatttgcc gacaggcgtg caatgcgcgc
     2401 agcgttagcc gcacacttat taatagtgtc tgtaaacatt tgattatcct ttaaaaaata
     2461 aaaacaaaaa aataaaacaa aaagtttaaa aaatgtcctg caatataccg aaggtaatta
     2521 caggaccatg caaaaagggg aggcattgcg cctcccgtta aaacattaac cgcgcagctg
     2581 caccacgccg tctttcactc gtgcttcgta atgtttgacg gaaaactgtt cgtcttccat
     2641 gcacaagcca tcacttaagc gaaaacgctg ttttttcagc gggctggcga cccacagctc
     2701 gccctggtgt tccgcaatca gcccgcgtga cagcacgctg gactcgaaga acggatcgat
     2761 gttgctgatc gcaaacacct ggtcgctgtg atacgggcgg aaaatcgcga cctgctcgtc
     2821 gcctaacagc gcgcagacgc cggtttcagg caggatgtca tcgattttgc agatgtcttt
     2881 ccactggctc atgcgttgtc ctccaccaga gttaccggga tacgttcata cggcgttgcc
     2941 ggacggtgct gttcgcgctc tggcaccatc tgcacgttcg ggtcacgctt gtcgctgttg
     3001 atgaagtgtt tgaagcgagt ctgcgcggac ggtgtattga ccgtttcagt ccactcacac
     3061 actaccgctt cacgcaggcg cgccatctct tcttccagat gagcgttcag ccccagtttg
     3121 tcgtcaatga tcaccgcttt caggtaatcg atgccgcctt cgaggttttc taaccacggc
     3181 gcggtacgcg tcagtttgtc ggcggtacgg atgtagaaca tcatgaagcg gtcgagatat
     3241 ttgatcagcg tttcgcgatc gatatcagct gccagcagat ccgcatgacg cggtttcatg
     3301 ccgccgttgc cgcaaacata caggttccag cctttttcag tggcgataat acccacgtct
     3361 ttaccctgag cttctgaaca ttcacgggta cagccggaga caccgaactt cattttgtgc
     3421 ggcgtacgga tgcctttgta gcggttttcc agttccacgc cgaggccgac gctgtcgcca
     3481 acgccgtagc ggcaccaggt gctacccacg caggttttcg ccatacgcag tgctttcgca
     3541 taggcatgac cggtttcgaa gccggcttca atcagctgac gccagatctc tggcagatcg
     3601 tctttctgtg cgccaaacat cgccaggcgc tgggaaccgg tgattttggt gtagagatta
     3661 aattcacgcg cgatacgacc cactgccatc agcccttccg gggtgatttc accgcccgga
     3721 gaacgcggga tcaccgagta ggtcccgtct ttctggatgt tagcgaggaa gttgtcgtta
     3781 gaatcttgca gcggagtatg ttctggcttc agaatgtatt cgttccagca ggaggccagc
     3841 agcgaaccga cggtcggttt acaaacttca caaccgtagc ctttgccgtg tttcgccagc
     3901 agttcttcga aggttttaat gccttcaacg cggatcaaat ggaacagttc ctgacgcgaa
     3961 taagcaaagt gttcgcacag gttgttgtta acttcgatgc cctgtttcgc cagttccgcg
     4021 ttcagtacct gagtgaccag cgggatacag ccaccgcagc cagtacccgc tttggtttca
     4081 gctttcagcg ccgcaactgt gtggcagcct ttgttgatgg cagcaatcag atcaccttta
     4141 gtgacgtcga agcaggagca gatttgcgcg ctgtccggca gtttatcaac accgatagac
     4201 ggcttaccgc tacccgagtg cgccggcaga atcagagaat ccgggttttc cggcagctcg
     4261 atagcattca gcaccagttg cagcaagtta ccgtagtcgc tggtatcgcc caccagtacc
     4321 gcaccgagca gggttttgtt gtcttcgctg acgatcaggc gtttgtagat ctctttgctt
     4381 tcgtcgaggt aaacgtagct acgtgcgcca ggcgtgcgac catgagcatc accaataccg
     4441 cctacgtcca cgcccagcag tttcagcttg gcgctaaggt cagcaccttc aaaggcgttt
     4501 tcgctaccga gaatatggtc aacggcgacc tgcgccattt tgtagccagg tgctaccaga
     4561 ccaaatatac ggttgttcca gcttgcgcat tcaccgatag cgtagatatc cggatcggaa
     4621 gtttggcagg aatcattaat gacgataccc ccacgcggag caacgtccag accacactgg
     4681 gttgccagct tatcgcgcgg acggataccg gtagagaaga cgataaagtc gacttccagt
     4741 tcgctgccgt cggcaaaacg catggtttta cgtgcttcga caccttcctg cacaatctca
     4801 agggtgtttt tgctggtgtg aacgcgcacg cccatacttt cgattttgcg acgcagctgc
     4861 tcgccaccca tctgatcaag ctgttccgcc atcagcatag gggcaaattc gataacgtgg
     4921 gtttcaatac ctaagttttt cagcgcgcca gcggcttcca gacctaacag gccgccacca
     4981 acaacggcac cgcgtttgct gcgacgggcg caggattcaa tggcgttgag gtcttcaatg
     5041 gtgcgataga caaagcagtc ctgagtatca gaacctttga ttggtgggat ccacgggtag
     5101 gaaccggttg ccatgatcag cttgtcataa aaaacggtac gtccggcgct ggagtgaatc
     5161 accttctcct gacggttgat ggtgatagcg cgttcgccga ccagaacttt gatgccgtgt
     5221 ttctcgtaga agccttcgcg caccagcgac agctcttcgg cggtgtggtg agagaagtaa
     5281 gacgagaggt gtacgcggtc ataagcgatg cgcggttctt cacagaaaac ggtaatatca
     5341 aagttggccg catcagattt atcaagaaga tcttcgataa agcgatggcc gaccataccg
     5401 ttaccgataa ttgcgagtct gactttgctc atttttgcct cgatttcttt tctattaccg
     5461 cctaccttaa cgattcagca accccgctta ttgatgtaaa tcaaattcac ctttatatac
     5521 cccttaaggg gtatattgct gatttgtata aattttctta agtcacggaa ttgtctgaca
     5581 tttcatgttt gtgcaaaaaa tagacaaaat gaacgggttt atagaaatag atgcacgtag
     5641 cggggttaga gaggaaaagg ccggacgacg atagtgccgt ccggcattag caattaatga
     5701 gaggtcaggg tgttatgctg acggtgacgg ctgacgaaac ctaacaggaa gcacatcaca
     5761 aagacgacag cgtacagacc gtttgctgta agcagtgccg cctgcggacc gctatgttca
     5821 acaatcgggc cggtaaccac aaaggtcaac atagtaccga tggttccgca ggtcaggaca
     5881 aagttaacca gttttggcga cggtactttg gtctgctgtg aacccagggt gatgatggtg
     5941 gtatagatcg cgctggagaa gaagcccaga gcgagaattg accacgccat atgtgctggt
     6001 gttccggtgt taaagacgta catcagaatc gcagccagac cagccagtac ggtcagaatg
     6061 cgttgcaaat caaagaagcg aagaataaag ctgaacgccc acatgccgac catgtatgac
     6121 atccagaagt tactcaccag cgtgcccgcg tcgttcaggc tcatgcccag gcctttggca
     6181 tactcaggca cccaggagat aaaacctaac tgaccgagga tgtagcacag cgccgcaaca
     6241 gagagaaaca gtacgccgat cccccacttt tctttctcta ccggagcatc cgtttttggt
     6301 gcatgtttgc ccagtgctgg gaactcacag ccgaaggtca gaataaaaat agcgacatac
     6361 accagcccga tgcaggcata aacccagtac cactcaatgc tgcgcgccag tagaaacgcg
     6421 gcgatcattg ggaaaatcat cccagccata ctgaagaagg agtcagtaaa taacaggcgg
     6481 gaaccgcgct gacgcccttc atacatttgt gttatcagga atgtaccaat cgacatagta
     6541 atgccgctga ccaccccgag aatgaacatc gccgtcgaga acagcgccag gctgtggctg
     6601 aacatcaaac cggcaaccgc cagcaccatc aggagaaagc caaaacgtaa ctgcgttttc
     6661 aacgggacga tttccatcag ccaggcgttg aggaagatag agattaaaat gccggcgttg
     6721 aggaaggtga aggtgttact catactggaa acaggcagat tgaaataatc ggcgatattt
     6781 cccatcacca tcccggtgac aataaccaac gcaccagtca gtgcgtagga gagaaagcta
     6841 atccatgtga gcttgatgcg attgctgtta gtcatgattg gcctgcgttc aaaaataaaa
     6901 tggcatagcg ggatatgccg cgagcgggtg attttaggtg attttgtgat ctgtttaaat
     6961 gttttattgc aatcggttgc taaattgcat tttaagaagt gatcttgatc acggaataaa
     7021 aagtgatcgt caggttacat atatttcaga tacgtaaaat taggtaaagg gatggccttg
     7081 ttcttgaagg ctatttagaa tctcttcact tgcttttttt ctgctctgtt tgttaaggaa
     7141 atctcatgtt caaatcgacc ctggcggcga tggctgctgt tttcgctctt tctgctcttt
     7201 ctcccgcagc aatggcagcg aaaggggccc cgcacgtatt gttgacaacc tcagctggta
     7261 acatcgaact ggagctggat aaacaaaaag cgccagtgtc tgtgcaaaac tttgtcgatt
     7321 atgtgaacag cggtttttat aacaacacta cctttcaccg cgtcattcct ggctttatga
     7381 ttcagggcgg cggtttcacc gagcagatgc agcagaaaaa accaaacccg ccaatcaaaa
     7441 atgaagccga taacggcctg cgcaacacgc gtggcaccat cgcgatggcg cgtaccgctg
     7501 acaaagatag cgccaccagc cagttcttta tcaacgttgc cgataacgcc ttccttgacc
     7561 atggtcagcg tgatttcggt tacgcggtat ttggtaaagt ggtgaaaggc atggacgtgg
     7621 ccgataagat ttcccaggtg ccgactcatg acgttggtcc gtaccagaat gtgccgtcaa
     7681 aaccggtagt tatcctttcc gctaaagtcc tgccgtaatg atttctcgcg cgggcaatct
     7741 tgcccgcgct tctgctctcc cggcgtaacc cggatttgcc gcttatactt gtggcaaatg
     7801 gacacgttca gggaggcatc aagtgaagaa actcaccgat aagcaaaagt cccgtctctg
     7861 ggagcttcag cgtaatcgta atttccaggc cagtcgccgt cttgaaggcg tcgagatgcc
     7921 tttagtcact cttactgccg cagaggcttt agcgcgcctt gtagagctga ggaggcacta
     7981 tgagcgataa attcggcgaa gggcgcgatc cgtatcttta tccaggcctt gatatcatgc
     8041 gtaaccggct gaacatccgc cagcaacaac ggctggaaca ggccgcttac gaaatgacgg
     8101 cgctgcgtgc tgcgaccatt gagcttggtc cgctggtgcg cggtttaccg catttgcgaa
     8161 ctatccatcg ccagctgtat caggatattt tcgactgggc agggcaactg cgtgaagttg
     8221 atatttatca gggcgatacg ccgttctgcc actttgctta tatagaaaaa gagggcaatg
     8281 ccctgatgca ggatctggag gaagaaggtt atctggttgg cctggagaaa acgaagttcg
     8341 tcgagcggct ggcgcattac tattgtgaaa tcaacgtgct gcatcccttc cgggtgggaa
     8401 gtggtctggc acagcggatc ttcttcgagc aactggcgat tcatgccgga tatcaactga
     8461 gctggcaggg tatcgaaaaa gaggcctgga atcaggcaaa tcagagtggg gcaatggggg
     8521 atctcaccgc actgcagatg atatttagca aagtggtaag cgaagccggg gaatctgagt
     8581 aaaatagcgc ggttctttcg taccggagcc gccatgatcc tgcttattga taactacgat
     8641 tcttttacct ggaacctcta ccagtacttt tgtgaactgg gggcggatgt gctggttaag
     8701 cgcaacgatg cgttgacgtt ggcggatatc gacgccctta aaccacaaaa aattgtcatc
     8761 tcacccggcc cctgtacgcc agatgaagcc gggatctccc ttgacgttat tcgccactat
     8821 gccgggcgct tgccgattct tggcgtctgc ctcggtcatc aggcaatggc gcaggcattt
     8881 ggcggtaaag ttgtgcgcgc cgcaaaggtc atgcacggca aaacctcgcc gattacacat
     8941 aacggtgagg gcgtatttcg ggggctggca aatccactta ccgtgacacg ctaccattcg
     9001 ctggtggtgg aacctggctc attaccagcg tgctttgacg tgacggcctg gagcgaaacc
     9061 cgcgagatta tggggattcg ccatcgccag tgggatctgg aaggtgtgca gttccatcca
     9121 gaaagtattc ttagcgaaca aggacatcaa ctgctggcta atttcctgca tctctgattt
     9181 ccgattgcca ttcagtgatt ttttatgcat attttgtgat tataatttca tatttattat
     9241 gcgtaacagg gtgatcatga gatggcaatt gaacaaacag caattacacg cgcgactttc
     9301 gatgaagtga tcctgccgat ttatgctccg gcagagttta ttccggtaaa aggtcagggc
     9361 agccgaatct gggatcagca aggcaaggag tatgtcgatt tcgcgggtgg cattgcagtt
     9421 acggcgttgg gccattgcca tcctgcgctg gtgaacgcgt taaaaaccca gggcgaaact
     9481 ctgtggcata tcagtaacgt tttcaccaat gaaccggcgc tgcgtcttgg gcgcaaactg
     9541 attgaggcga cgtttgccga acgcgtggtg tttatgaact ccggcacgga agctaacgaa
     9601 accgccttta aactggcacg ccattacgcc tgtgtgcgtc atagcccgtt caaaaccaaa
     9661 attattgcct tccataacgc ttttcatggt cgctcgctgt ttaccgtttc ggtgggtggg
     9721 cagccaaaat attccgacgg ctttgggccg aaaccggcag acatcatcca cgttcccttt
     9781 aacgatctcc atgcagtgaa agcggtgatg gatgatcaca cctgtgcggt ggtggttgag
     9841 ccgatccagg gcgagggcgg tgtgacggca tcgacgccag agtttttgca gggcttgcgc
     9901 gagctgtgcg atcaacatca ggcattattg gtgtttgatg aagtgcagtg cgggatgggg
     9961 cggactggcg atttgtttgc ttacatgtac tacggtgtga cgccggatat tctgacttct
    10021 gcgaaagcgt taggcggcgg cttcccgatt agcgccatgc tgaccacggc ggaaattgct
    10081 tctgcgtttc atcctggttc tcacggttcc acctacggcg gtaatcctct ggcctgtgca
    10141 gtagcgggcg cggcgtttga tatcatcaat acccctgaag tgctggaagg cattcaggcg
    10201 aaacgccagc gttttgttga ccatttgcag aagatcgatc agcagtacga tgtgtttagc
    10261 gatattcgcg gtatggggct gttaattggc gcggagctga aaccacacta caaaggtcgg
    10321 gcgcgtgatt tcctgtatgc gggtgcagag gttggcgtaa tggtgctgaa tgccggaccg
    10381 gatgtgatgc gtttagcacc gtcgctggtg gtggaagatg cggatatcga tgaagggatg
    10441 caacgtttcg cccacgcggt ggcgaaggtg gttggggcgt aaataagacg catcaggcaa
    10501 aatatgtcgg atgcggcgtg aatgccttat ccgatggttt agcctgtatt acgccttcga
    10561 atcccgcaac ttgcgactca gccaaatccc gtgatgcggt cgctgacgcc atgccatcga
    10621 cgaaatggtg tgcatggtgt tcagatgacc aataacccgc tgtaaatgct gctccagcgt
    10681 acctgccgcg ccttcgtgcg gctgcatctc cggcgcatcc atgatattgg catcgccaga
    10741 actacccggt tcgtcatact ccagtcgctg ctgacaacgc tgaatggcga tttcacaaga
    10801 ctgtaaatac tcttgtgcca gttcaggtgg caatgcccgg tgttcccgcg ccagcgtggt
    10861 catggcatta atatgctcaa caataaactg gctgtgcgtt acccacagtt tcatatctgc
    10921 cagataatgg ctgttaaacg ccggttcctg catcgcctga ttcaatgagt tatacagagt
    10981 gttatgtgcc tgatttaccc gcattcgctg ccaggccagt ggcgtaggtt gcggatcctc
    11041 gctaagaatc aagcgaatcg cttcctgata ggcttctaaa gcgtcatggg cgtttttacg
    11101 caataacccg ctctgccact gcggccacag ccagacagta ccgccgaaag caattaaaca
    11161 accaataatg gtatcgataa gacgcggaag gatgtattgc tcgccgttca accacaatag
    11221 ttgcagggta tacactgcgg taatagtaaa accgaccgtc gcccagccgt agtttttgcg
    11281 caatatcagg tagctggcga gggtggtaat cagcatcaac gtcagggtgt aaccttcggg
    11341 aattttaaag tgcagcgcca cgcccgcaat gattaacccg accacggttc ccacggagcg
    11401 attcacaatc ctcagacggg ttgcgccata gccattttgt gtcaccagca ataccgtcat
    11461 caggatccag tacgacttcg gcagatgcag cgcggtgccc atcaggctgg caacgcttaa
    11521 catcacactg agtcgtccgg cattgcgtag cgccggagac tttagtgaca gataactttt
    11581 cagcgcagga agtaatggca ttcgccgctg tttatcggcc agtaagtcac gggcatagag
    11641 cggtttttgg gtgcgcagca cgcgggcgat gcggctgaaa tgccagtagc agaattgccc
    11701 aaccggatta tccggatgct ggcgggcgat tttttccagt gcgccaattt gcttttccat
    11761 cgtaaaacgc gttggcaggc gatggtaaag aatgtcatca gccagcacgc gcaggcgagc
    11821 ggcgacggtt tgcgcattcc agcggataac ttcttccgca tggctacgct cgaccagctt
    11881 ttgcacctct tccggctgat gcaaactgac cgaaatatgt tcctgtaaat ccagcgcctc
    11941 ctggaaaata cgcagcatcc gcttgtagtc agtattattt tgcgcggaaa gcatatgcat
    12001 ttgctgatag cactgggtaa ttagatcgac cgctttttgc tggcgcacca gcagcggcgg
    12061 cagcgctttt tcagggtcgg tgtgctgggt aagcaggctg tatttggctt cacaataatc
    12121 tgccagttca cggtacagca gacttagtga ctcgcgcagc ggttgttcgc gccagatcca
    12181 gaaccaaaac cagttaaaca atccgtacca gagagtgccc aacgcataga tgagcaacgg
    12241 ttcccagacc ggcatgtatc ccgccagact gagggtaaaa atggctgcca gcagcgatgc
    12301 gggaagcaat tttgcgtgca atggccccaa ctcagcagtg acgccaagta ccagcgttaa
    12361 tccggtcagc aaaaagggca ggggaacatc ttttgccagt agcagctgtg tcagtaagct
    12421 acaggtggca aacagcgacg caccaatgat taagcgtttg aaaaaacgtt tatgaggcgt
    12481 atcaaggccc gcaatattgc aacaggcagg aacgagagag aagagcagac cgaatcgtaa
    12541 ttcgccaagc attaacccaa cggccacggg caaacatagc accagcgttt gtcgaagtgc
    12601 atagttgata tcggggtgat aaatcagtct gcgccacatc gggggaaaca aaatggcgcg
    12661 ctaccaagta acgcgccact ccgacgggat taacgagtgc cgtaaacgac gatggtttta
    12721 ccgtgtgcgg agatcaggtt ctgatcttca agcatcttca gaatgcgtcc cacggtttca
    12781 cgagaacagc cgacaatctg accgatttcc tgacgggtaa ttttgatttg cataccgtcc
    12841 gggtgagtca tagcgtctgg ttgttttgcc agattcagca gagtctgtgc aatgcggccc
    12901 gtcacgtcga ggaacgccag gttgcccact ttctctgaag tgacttgcag acgacgcgcc
    12961 atctgtgcag acagacgcat cagaatgtcc gggtttacct gaatcaattg gcgaaatttt
    13021 ttgtacgaaa tttcagccac ttcacaggcg gttttcgcac gtacccatgc gctacgttcc
    13081 tggccctctt caaacaggcc cagttcgcca ataaaatcac cctgattcag ataggagagg
    13141 atcatttctt taccctcttc gtctttgatc agcactgcca cagagccttt aacgatgtag
    13201 tacagcgttt ccgctttttc accctggtga ataagcgtgc tcttggatgg gtacttatga
    13261 atgtggcaat gagacaagaa ccattcgaga gtcgggtctg tttgcggttt gccaagcacc
    13321 atgcgcggtt atcctctgtt ataagctttc tccagagcca gataacgccg ctgtctctgg
    13381 attgccgaaa tatgcttccc gctacctggg aaggggctat caactgtact gcacggtaat
    13441 gtgacgtcct ttgcatacat gcagtacatc aatgtattac tgtagcatcc tgactgtttt
    13501 agcatagctt tcgctttgtg tctcctggtg tctcgcttca gcatgaccca ggtcgccttc
    13561 cgttgcgcga tttggttagt acg
//
LOCUS       DADLTD010000109        13520 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLTD010000109 DADLTD010000000
VERSION     DADLTD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.109"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..186
                     /locus_tag="JN701_002523"
     CDS             <1..186
                     /locus_tag="JN701_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7364937.1"
                     /translation="CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELL
                     NRRLSESVPCMPHKKAG"
     gene            207..908
                     /gene="mlrA"
                     /locus_tag="JN701_002524"
     CDS             207..908
                     /gene="mlrA"
                     /locus_tag="JN701_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HAY7364938.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            968..1075
                     /locus_tag="JN701_002525"
     CDS             968..1075
                     /locus_tag="JN701_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY7364939.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="JN701_002526"
     CDS             complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="JN701_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY7364940.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="JN701_002527"
     CDS             complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="JN701_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY7364941.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="JN701_002528"
     CDS             complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="JN701_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY7364942.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="JN701_002529"
     CDS             complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="JN701_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY7364943.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPVFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="JN701_002530"
     CDS             complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="JN701_002530"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY7364944.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7537..9252
                     /gene="dld"
                     /locus_tag="JN701_002531"
     CDS             7537..9252
                     /gene="dld"
                     /locus_tag="JN701_002531"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7364945.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="JN701_002532"
     CDS             complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="JN701_002532"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7364946.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10397..10984)
                     /locus_tag="JN701_002533"
     CDS             complement(10397..10984)
                     /locus_tag="JN701_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY7364947.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11154..11732
                     /locus_tag="JN701_002534"
     CDS             11154..11732
                     /locus_tag="JN701_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7364948.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11862..12623)
                     /locus_tag="JN701_002535"
     CDS             complement(11862..12623)
                     /locus_tag="JN701_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7364949.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12676..>13520)
                     /gene="mdtQ"
                     /locus_tag="JN701_002536"
     CDS             complement(12676..>13520)
                     /gene="mdtQ"
                     /locus_tag="JN701_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY7364950.1"
                     /translation="AALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINASK
                     TRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRAD
                     LQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTL
                     PIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIE
                     RDTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGG
                     GYKR"
BASE COUNT         3241 a         3779 c         3411 g         3089 t
ORIGIN      
        1 tgctggtcac ttgccgcagg agctaatcct agttttatcg caagccagat gggccacaca
       61 aacgcacaaa tggtattcaa tgtttacgga gcatggatga aagacaacaa tcacgaacag
      121 atagaacttc ttaacagaag actatctgaa agtgtcccat gcatgcccca taagaaagcg
      181 gggtaaaata aaaacttgca aaatcaattg gtttaccttt aatccctgtc acgttacgcg
      241 cgtggcagag gcgttacggg ttgctgaaac cgcaacggac agacggcggt catcgactgt
      301 tcaacgatgc cgatattgac cggatccgcg agatcaaacg ctggatcgac aacggcgtgc
      361 aggtcagcaa agttaaaatg ctgctcagta atgaaaatgt tgatgtgcag aacggctggc
      421 gcgatcagca agaaacatta ctgacctacc tgcaaagcgg caatctgcat agcctgcgaa
      481 cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca catctgttta
      541 ttcctctgcg ccgacggctt cagtgccaac aaccgactct ccaggcgctg ctggcgatcc
      601 tcgacggcgt actgatcaac tacatcgcca tttgtctggc ttcggcacgt agaaaacagg
      661 gtaaagatgc gctggtggtt ggctggaata ttcaggatac cacccgtctg tggctggagg
      721 gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg ctcaatcaac
      781 tacgccctga actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa aatcgaacct
      841 ccgcccaaca gcagcaactc accagttggc aagaacaagg ccatgatatt ttcccactcg
      901 gcatttaatg attcgttaac aaatgcgctt tactgtacaa tcctttcgtt aacataagga
      961 gtgcaatatg cgcatagcta aaattggggt catcgccctg ttcctgttta tggcgttagg
     1021 cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg gctaagcgcc
     1081 tgcaccagcc tttcaaacag gcggtctgca atgatcgccg ccagtgccac cagtaacgcc
     1141 ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatggg cgtacccagc
     1201 gtgctggccc ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat caccgaagtt
     1261 cgcacgcccg ccagaatcac cggagccgcc agcggtagct cgaccttacg cagtcgctga
     1321 ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcaat cgctcccagc
     1381 ccggcaagtg tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc gataatcgct
     1441 ggttgcagac caaagccgat caccggaacg gcaatcgcca gcactgcgac aggcggaaaa
     1501 gtctgtccaa cggcggcaat agtttccacc agtgggcgaa attccgcgcc ccacgggcga
     1561 gtgacagcaa ttccggcacc agtgccaatg atcaccacaa acaaactcga aattcccacc
     1621 agccagaaat gagccagtgc cagagctgca aaactttctt gctgataaac gggtcgtggc
     1681 agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa aatcagtgcc
     1741 acaaacaaag caatgagcca gaacagcgga tcgcgcaaca tcttcatacg cttacgcctc
     1801 ctccagcaga tcctgaaaat gcagcgtgcc gcaaggctgg ccctgcgtgt tcaccaccgg
     1861 cagcacctcg catccccgcg cgacaaacag ggagagcgca tcgcgtagcg tcatctcttc
     1921 tgccagtgcc tcaccttctg cccgttcttc gcgacacacg taatccgcca cactacgtaa
     1981 cgaaagcagg cgcacaccca gttcgctgcg cccaaaaaac tggcggacaa aatcgtttgc
     2041 cggacgagtc agcatcgtca gcggattgcc ctgctgcacc acttcaccgt gatccatcaa
     2101 taccagatgt tctgccagcc gtagcgcctc atcaatatca tgagtgacca gcacaatggt
     2161 acgccccagc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc gcgttaccgg
     2221 gtccagtgcg ccaaaaggtt catccatcag taagacttgc ggatcggcag ccagtgcgcg
     2281 cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac gctcacgtaa
     2341 atttgactcc agccccagta gcgccattaa ttcgtcgata cgatcgtcaa tccgcgcccg
     2401 cgaccatttt tgtaattgcg gcacggtagc gatgttttgc gccacgctcc agtgggggaa
     2461 caggccaata gattgaatgg catagcccat ccggcggcgc aactccagta ctggcagcga
     2521 gcgaatttct tctccggcaa agcggatcac tccgctgtca tgctccacca ggcggttaat
     2581 cattttcagg gtggtggatt tgccggagcc agatgtgcca atcagcaccg aaaaactccc
     2641 ttcctgaaaa ttgagattga gatcgttaac ggctttttgt gcgccgaaca gtttgctgac
     2701 atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa tcgaacagcg
     2761 cgtcggtaag caccgccaga acaattaccg ggatcacccc cagcaacact aaatcaatgg
     2821 cgctgcttag cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg attaacgccg
     2881 caatcaccgc catacctaca gtttgcacca tcaccacccg caggctgcgc agaaataccg
     2941 gtaacgccag tggtaactga acatgcagga atcgctgcgc cccgctcatg cccatcgctc
     3001 tggcactctc cagcacatcg cgcgggatct ggttcaaacc gactaccacg ccgcgcacca
     3061 gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg gttcctgcta
     3121 tgccgagctt ccccagccac ggaaaggccg tcaccagcgc ggcaagcggc gcaatcaaca
     3181 ggccaaagag cgccaccgaa ggcacggtct gaatgacatt gagcagagaa aaaattgccc
     3241 cctgccgagc agtggaaaag tagcaccaga tgcccaacgg cacaccaatc actaacgcag
     3301 gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca aacacatcct
     3361 gacggttggc gtattctttc attagtgaga gatcgttaag cgtgccggag tacagcaacc
     3421 acagcggaat aatggcaatc tgcatatgca acaaccagcg ccacagcgga tgcgtggaga
     3481 ttcggcggat ggcatcgcta caggccagca atgccagcgc cgcagccagc cagaaaccac
     3541 tgccgaggct ggtacgcgcc agcgcactgc cattttgcgc cagttgtgtc gccgcctttc
     3601 ccgcgcccca caccagcaat acgaagacga attgcgccag aatgagtgca caaatgctgc
     3661 cttttttacc gggaataaaa caggccgtca gccaggcgca accaacgccc accagcatcc
     3721 agatcgtttg cggccacagc tgccagagat gacgcccctc acccgaaact aaacgattag
     3781 gcgcgtaact gataaacggc agcgccgctg cgattgccgt cagcaacagc agcagcgcca
     3841 gaacaggatt aatacggaaa taagtcacgg gaaattactt cgtccaccct ttttgtttca
     3901 ggtagtcggc agccactttt ttggcatcca gtccttccac agcaatgctg gcattcagtt
     3961 gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt gccatttgcg
     4021 gatactcctt caacaccgac tcacgcacca ctggtgcagg cgcgtagata ggttgcacac
     4081 cttgcggatc gcttaaggtt tgcagcccca gcgccgctac cgggccgtca gtgccgtaag
     4141 ccattgcagc attaacgcca gagatttgct gggcagcggc tttgatcgtc accgccgtgt
     4201 cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag gctttttcaa
     4261 acacgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt ttgaaggtgc
     4321 cgccctcttt cagataacga ctcaggtcgg caagcgaagt gagtttattt ttctctgcca
     4381 catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc agccagatta
     4441 acttgttgtg ctccgaatcg agctttttga ctttctcgta accttgctgc gcgtttttcc
     4501 acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat tccggataga
     4561 tatccagttc accggaagta atcgccccgc gcaccacagg agtcgttccg agttgcactt
     4621 tattcaccgt cggtacgccg tggctttcca gcacctgcaa gatgatgttg ccgagtagcg
     4681 cgccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt agcggcaggc
     4741 tcacggctgc caacataacc agtgaacctg cccagacctt tgagagtggc atgatgcttt
     4801 cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg ataatgacag
     4861 agttagcctg agcggaagga ttcagttgca gaatcagata aatacttaaa gggcatattt
     4921 tgcccggcgt ttcttgccgg atgcggcgta aacgccttat ccggcctaca aaggtcgaaa
     4981 attcagcaag ttaagatatg aacatcaggc aaagggcgca aacgtcgcgc ccttactaaa
     5041 gcattacagc aactcaaact cgcctttctt aacgcgtgcg gaatcagtgc cgataaagac
     5101 attgaacttg ccaggctcgg cgtcatattt catctgttga ttccagaact tcagcgcctc
     5161 gatatcaatc gggaagctga cagtctgagt ttcgcccggt ttcagggtga ttttctcaaa
     5221 gcctttcagc tgcttcactg gacggctcat ggaagccgtc acatcctgca agtacatctg
     5281 tactaccgtg gccccttcgc gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt
     5341 gccgtcacgc ttcatggtcg gcgcagaaag tttcacatca gagacggtga aagtggtata
     5401 gctcagacca tagccgaacg gataaagcgc cccgttagct tcatcaaaat aacgcgaagt
     5461 gtatttgttc ggcttgtcgg cattatacgg acgaccggtg ttcagatggc tgtagtacac
     5521 cgggatctgc ccgacagaac gcgggaagga catcggcagc ttgccggacg ggttgtaatc
     5581 gccaaacaac acatcggcaa ttgcattacc gccttcagtc cccgcaaacc aggtttccag
     5641 aatcgcatca gcctgctgat cttctttcac cagcgccagc ggacgcccgt tcatcagcac
     5701 cagcaccagc ggtttaccgg tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg
     5761 aatagtgata tcggtacggc tggaggcctc gtgcgccatc ccctgtgctt caccgaccac
     5821 agccaccacc atatcagatt gtttcgccgt ctgcaccgct tcatcaatca tctcttgcgg
     5881 cgagcgcgga tcgactttga ccgcttcttc atactgattc aggaaatcga taatgccttt
     5941 gtcactggta acgttcgccc ctttggcata cagcacttta ccgttttcac cgacggcatt
     6001 tttaatcccg gtcagtacgg tcacggattg atcggcaaca ccggctgcgg accagctgcc
     6061 catcacgtcg cgtttactgt ccgccagtgg cccaaccacc gcaatggtgg ccgatttttt
     6121 cagcggtaac gtttcgagac ggtttttcag caacaccagg ctttcgcgcg ccacttcacg
     6181 tgcttcttta cggtgcaggc ggctttcggc attggtatcc accgggtcag actctttcgg
     6241 ccccaaatgg ctgtatgggt cgttaaacaa tcccatatca tatttaacgt tcagtacatg
     6301 gcgggcagca tcgtccagct cttccatcgt cactttgccg gatttgatca acccaggcag
     6361 atacttcgag taatactcgt cgctcatact catgttgatt ccggatttca gcgccacgcg
     6421 caccgcatct tccgggtctg ccgccgtgcc atgtttaatc agctctttga ttgcaccgtg
     6481 atcggaaacg gtgatgcctt taaagcccca ctggtcgcgc agaacatctt tcagcagcca
     6541 ggaatcggag gtggctggcg tgccgttcag cgagttcagc gccaccatca ccgcgccgct
     6601 gcctgcgtcc agccccgctt tgtacggcgg catataatca ttaaacaggc gctgcggact
     6661 catatcgacg gtgttgtact ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt
     6721 gacgctggtc atcaccgagt agcgatctgc cgggctttta ccctgcatcg cttccaccat
     6781 ggttttaccc attgttgagg tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc
     6841 ccagcgcgga tcgcgcgaga catcgaccat cggtgcccag gtcatattca ggccatcatc
     6901 tgccgcttca taagcagaga cacgtccgac cgttttcact gcatcgaggt taaaagacga
     6961 ggccagaccg aggctaatcg ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa
     7021 gaaaagagga attttcaggc ggcttaattc catcacctga tcctgcatgg cgcggatatc
     7081 ctgacgggtt acggtgttga aaatcgcccc aacctgaccg tctttgatca tctcgcggat
     7141 cgcctctttc gggttatccg ggccgacgct gattaagcgc agctgaccaa ttttctcatc
     7201 aactgtcatt ttcttaagca attcggtgac gaacgcatcc cgcgcttcgg gcgtgaatgg
     7261 atggttgccg aataaatcat ccgccagtgc tggctgcagg gccagactca ccgcgattcc
     7321 tactgaacat agccatttca tatggatttt tcttcctctg ttgcccgacg aggcagcgaa
     7381 aatggtgcaa aaaccgtagt ttgccataag catgatggag agagaaaaag aatgctcagt
     7441 ttattgtctg aattttcaaa atattcactc gctgaattgt catacaaggc gctattctag
     7501 tttgtgatat tttttcgcca ccacaaggag tggaaaatgt cttccatgac aacaactgat
     7561 aataaagcct ttttgaatga acttgcccgt ctggtcggtc attcacacct gctcaccgat
     7621 cccgcaaaaa cggcccgcta tcgcaagggc ttccgttctg gtcagggcga cgcgctggct
     7681 gtcgttttcc ctagctcact actggaattg tggcgggtgc tgaaagcctg tgtcaccgcc
     7741 gacaaaatta ttctgatgca ggctgccaat acaggcctga ccgaaggatc gacgccaaac
     7801 ggtaacgact atgatcgcga tatcgtgatc atcagcaccc tgcgtctcga caagctgcac
     7861 gttcttggca agggcgaaca ggtgctggcc tatccgggca ccacgctcta ttcgctggaa
     7921 aaagccctca aaccgctggg acgcgaaccg cactcagtga ttggatcatc gtgtataggc
     7981 gcatcggtca tcggcggtat ttgtaacaac tccggcggct cactggtgca acgtggcccg
     8041 gcgtataccg aaatgtcgtt attcgcgcgt ataaatgaag acggcaaact gacgctggtg
     8101 aaccatctgg ggattgatct gggcgaaacg ccggagcaga tccttagcaa gctggatgat
     8161 gatcgcatca aagatgacga tgtgcgtcac gatggtcgtc acgcccacga ttatgactat
     8221 gtccaccgcg ttcgtgatat tgaagccgac acgcccgcac gttataacgc cgatcctgat
     8281 cggttatttg aatcttctgg ttgcgccggg aagctggcgg tctttgcagt acgtctagat
     8341 accttcgaag cggaaaaaaa tcagcaggtg ttttatatcg gcaccaacca gccggaagtg
     8401 ctgaccgaaa tccgccgtca tattctggct aacttcgaaa atctgccggt tgccggggaa
     8461 tatatgcacc gggatatcta cgatattgcg gaaaaatacg gcaaagacac cttcctgatg
     8521 attgataagt taggcaccga caagatgccg ttcttcttta atctcaaggg acgcaccgat
     8581 gcgatgctgg agaaagtgaa attcttccgt ccgcatttta ctgaccgtgc gatgcaaaaa
     8641 ttcggtcacc tgttccccag ccatttaccg ccgcgcatga aaaactggcg cgataaatac
     8701 gagcatcatc tgctgttaaa aatggcgggc gatggcgtcg gtgaagccaa atcgtggctg
     8761 gtagattatt tcaaacaggc cgaaggcgat ttctttgtct gtacgccgga ggaaggcagc
     8821 aaagcgtttt tacaccgttt cgccgctgcg ggcgcagcaa ttcgttatca ggcggtgcat
     8881 tccgatgaag tcgaagacat tctggcgttg gatatcgctc tgcagcgtaa cgacaccgag
     8941 tggtatgagc atttaccgcc ggagatcgac agccagctgg tgcacaagct ctattacggc
     9001 cattttatgt gctatgtctt ccatcaggat tacatagtga aaaaaggcgt ggatgtgcat
     9061 gcgttgaaag aacagatgct ggaactgcta cagcagcgtg gcgcgcaata ccctgccgag
     9121 cataacgtcg gtcatttgta taaagcaccg gagacgttgc agaagtttta tcgcgagaac
     9181 gatccgacca acagtatgaa tccggggatc ggtaaaacca gtaagcggaa aaactggcag
     9241 gaagtggagt aaaaattacg gatggcatag tatcgccatc cgtcattcac ttaatcgttc
     9301 tgtgccgtct gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct
     9361 gctgctggaa caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg
     9421 gcaaaatggg tatatttgcc aaacgcatcc atcactacca gcgccaccgg tttattattg
     9481 ataaccgtac gcatcaccag acaatggccc gccgcattgg taaaaccggt tttggttaac
     9541 tgaatattcc agttatcgcg atacaccaga tggttagtat tgcggaacgg cagcgtatac
     9601 gtcgggttag agaaggttgc catatcttcc cgggtagtac ttaactgccc gatcaacgga
     9661 tattgtttgc tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc
     9721 gacaatccgg taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca
     9781 ttcattgcct taataaaggc tttgtaacca ccgggataat ggtgtgcaag gcttgccgcc
     9841 gcgcggtttt ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta
     9901 ttcagtcgta cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt
     9961 agtttttcat ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg
    10021 atagacgcaa tcggacgcac cagatccggg tggttcgagt agatcacttt gttggtattc
    10081 agatcaacaa tcatcgcgct accggaggca atttcaggtt gtgaagcggt gatagcggct
    10141 gccgttttcg caacggcctg cggtgccaaa ggcacagcca gcatcagggc caggctaaat
    10201 aaagaaactc gaaatttcgg catgatgagc attcagatag tggttcacgc gcacaggttg
    10261 cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg
    10321 cgatcgtcaa aggagaatcg tgaggaaatg ctgcattgct gacatttacg ccagcaatgc
    10381 aacgtcaaag aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg
    10441 cgagcagcac ttccagtacc agcacgccaa tcgccagcgt cgaactagag aagctaaggc
    10501 cttcctcttt gttgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt
    10561 aaaacagcgc caccgtgccg accagcgcac acaaccagac cagtggataa agcgccacca
    10621 aaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg
    10681 acggacgctg cggataatta cgcgccatcc accagatgac ccgccccatt accgcaaccc
    10741 cagccagcat aacgccataa aacaggacag ccagcgccag tccggtaaac caggataact
    10801 tcaggatagt gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa
    10861 tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt
    10921 tttcgcgatt aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc
    10981 tcataccgcc cccttgttgt gagttcatga acctgacagt tcaagtataa gtcagcttgt
    11041 gattattttt tgttcactca tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg
    11101 aatgatttcc agggtgtatg cttacaggca accaaaggga gacagactgg cctatggatc
    11161 tcaatacact catctcacaa tatggttatg ccgcgctggt gatcggtagc ctggcggaag
    11221 gtgaaaccgt gactttgctg ggaggcgttg cggcacatca ggggctatta aagttcccgc
    11281 tggtggtact ttctgtggcg cttggcggca tgattggcga ccaggtgctt tatctgtgcg
    11341 ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc
    11401 gggcgcagaa acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt
    11461 atggctttcg ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct
    11521 ttctgccgct gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt
    11581 acgctggtgg tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact
    11641 gggtctggtt gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc
    11701 gcgggaagaa aaagccggat catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg
    11761 ccgcatccga catccggtgc accatgcctg atgcgacgct tgtgcgtctt atcatgccta
    11821 ccaaaatttt acccattgaa ataaccacgc acctcactgc gctattctgg gttgaactgc
    11881 ggattcgcca acataaagcc gccatccact atcaacgact gcccggtggt gtaatttgcg
    11941 ccctctgagc acagccacgc taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc
    12001 agaggaatcg aaggctccgc gtcgggcttc acgtcgctgt catccatgcc attcattggc
    12061 gtggcgatcg ccccaggcgc gactgcgttc accaaaatct tatgcctgac cagctccagc
    12121 gccatcgctt tggttaaccc accgagcgca tgtttagcgg ctgtgtaggc gctggcatcc
    12181 ggcagcggcg tatgttcatg taccgatgta atgttgatga tgcgaccgcc ctgcccttgt
    12241 ttcaccatct ggcgggccgc aatttgtgag cataaaaatg caccatcgac atcaacggta
    12301 aaaatcttac gccactcatc gaaagccata tcaagaaacg gcgctttggt cattgcgccc
    12361 gcattattca ccagcacatc aatacgcccc agccgttgaa tgagtttctc cagcgcctgc
    12421 gcaccttctg gcagtttacc gagatccagc tgcacgatct ccgcacgtac gccgtggcta
    12481 actacctcac gcgcggtatc ttttgcccct tcttcatctg agtgccaggt aatgccaata
    12541 tcaaaccctt gctgcgccag taataacgcg cactctttgc cgatccccga atcggaggcg
    12601 gtaataatcg caacctgtgc catcgagttc tccacttaac gctgaataaa cgttaagtat
    12661 agaaggcgca tatcatcagc gtttgtaacc cccgcccaac gcaccagtga gttgaatgga
    12721 ggcatccagc cactgccctt gcaataacag gccattggcc cgctcacgca gcgcggggat
    12781 tctggcttcg ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag
    12841 acctaccaca cgcaaggtat cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag
    12901 tgtctgaacc tgactggctg cccgcgccac gtcattcacc gcttcaacca ccgctttgtt
    12961 gtagctggcg atagacaagt tgctttcggc ttttgcgata tcgagattgg cgttaagacg
    13021 accactatcg aaaatgggta gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg
    13081 acggaacaga tcgcttaagt gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc
    13141 aggataaaat gccgcttttg ccgcatcaat ggtgcttagc gatgactcaa cgtaccaatg
    13201 cgccgcctgc aaatctgccc gccgggccag taaggagtac cccagttcat caggaagctg
    13261 gcttgccact ttcggcaacg cgaccggttt aagcttcaat gactttgtct ggttatttgt
    13321 aagtgcgctt aaccgtgcct caataatttt cattttcccc gcgacatcgt tgagctgctg
    13381 ccgggttttg ctggcattaa tatcggtttc cacaccttca actgaagaag taatcccgtt
    13441 ctgatatagc tggcgatcgg tcgcgataat ggtgttctgc tctttttcta tttgctgcaa
    13501 gaccgtgttt aacgccgcct
//
LOCUS       DADLTD010000110        13490 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLTD010000110 DADLTD010000000
VERSION     DADLTD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.110"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            558..1646
                     /locus_tag="JN701_002537"
     CDS             558..1646
                     /locus_tag="JN701_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY7364951.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            1657..3936
                     /locus_tag="JN701_002538"
     CDS             1657..3936
                     /locus_tag="JN701_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364952.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKHLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASIADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSLAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAVMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            3929..5065
                     /locus_tag="JN701_002539"
     CDS             3929..5065
                     /locus_tag="JN701_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7364953.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWFNSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIPSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            5062..7062
                     /locus_tag="JN701_002540"
     CDS             5062..7062
                     /locus_tag="JN701_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364954.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            7187..7648
                     /locus_tag="JN701_002541"
     CDS             7187..7648
                     /locus_tag="JN701_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY7364955.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(7689..8159)
                     /locus_tag="JN701_002542"
     CDS             complement(7689..8159)
                     /locus_tag="JN701_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY7364956.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(8206..8925)
                     /gene="yehT"
                     /locus_tag="JN701_002543"
     CDS             complement(8206..8925)
                     /gene="yehT"
                     /locus_tag="JN701_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY7364957.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(8922..10607)
                     /gene="btsS"
                     /locus_tag="JN701_002544"
     CDS             complement(8922..10607)
                     /gene="btsS"
                     /locus_tag="JN701_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY7364958.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            10829..10894
                     /locus_tag="JN701_002545"
                     /pseudo
     CDS             10829..10894
                     /locus_tag="JN701_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            11122..11370
                     /locus_tag="JN701_002546"
     CDS             11122..11370
                     /locus_tag="JN701_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7364959.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(11486..11755)
                     /locus_tag="JN701_002547"
     CDS             complement(11486..11755)
                     /locus_tag="JN701_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7364960.1"
                     /translation="MLLQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIAKEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
     gene            11812..12480
                     /locus_tag="JN701_002548"
     CDS             11812..12480
                     /locus_tag="JN701_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7364961.1"
                     /translation="MVKLMKKNTDDGAKIYTPITLKLYDWWVLGVSNRLAWGCPTTEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFETYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            12796..>13490
                     /locus_tag="JN701_002549"
     CDS             12796..>13490
                     /locus_tag="JN701_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7364962.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         3427 a         3449 c         3282 g         3332 t
ORIGIN      
        1 ctgtattccc ccttagatgc gtttcaagtt gcccgttact tcaacaaatc aatcaaccgt
       61 aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg tgtcagcgtg
      121 tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac actgcctgtc
      181 gatactggtt tacgtatgtt ggaccacgat gttgcactcc ggatcagggc accacctgca
      241 gcaccacccg tccggtagcc aggacttgaa atagattcga tccggcgcaa cgtcccaacg
      301 gtctggatta taaatctcat tatcttaatt gcaacggggt ccagccgtgg ttataattcg
      361 ttcattggaa cacgatgttg cacaggctgt ggtgttaggc ctgaaagtag taaataaaag
      421 aacctgcctc accagcaggt ttttttattt actgtgatct gctttccaga tatttttcgc
      481 tcaaacaact aatgcgccaa acatttattg cgcgtaaaat atcgtttatt tcattaatac
      541 atttcaggga tgaatatatg tcaccgcaga ataatcatct gcagcgtccg cctgctgctg
      601 tgttatacgc cgatgaactg gcaaaattaa aacaaaatga taacgcacct tgcccgcccg
      661 gttggcagtt aagtttgcct gcggcccgtg cttttatcct tggcgatagc gcgcaaaata
      721 ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg tatgttagtc actcttgcca
      781 ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc aaaatctctc ctgtctgaat
      841 tactggcaac cgctattagc ggcgatgccg ggttaaccat ccagggcggc gcatctacta
      901 ccgaagatca aatcaagtac ggctggaact acgccctgct tatcaatcat ggtccatcaa
      961 cggaagcgct ggtccctgcc ccgctttacc agggaatgcg cgacggcaaa atcgtccgtt
     1021 ttgaagagat aacgcgtaca ccgctggaag tgcaagactg tctgttaggt atgctttctg
     1081 acagggtgat gacggtgccg gaactcactg gtgaagccag tcagctctat gcgcgagaag
     1141 gctttaatat tattgccact gccaataccc gcgatcgcgg cgtcaatgaa atgagtgctg
     1201 cgctaaagcg tcgcttcgat tttgaaactg tctttccgat tatggatttc gcccaggagc
     1261 tggaactggt cgccagtgct tcggcgcgtt tgctggctca tagcggtatt ccgcataaag
     1321 tgcctgacgc ggtactggag ttactggtcc gcaccttccg cgatctgcgt gccaacggcg
     1381 aaaagaaaac gtcaatggat acactaacag cgattatgtc caccgctgaa gccgtgaacg
     1441 tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa tcgcgcaggt gagccagccg
     1501 acctggtgga ctgcattgcc ggaaccatcg tcaaagataa cgaagaagat cgcgctcgtc
     1561 tgcgccgtta cttcgaacaa cgagtcgcca cacataaaga agctcactgg caggcttatt
     1621 atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt aattgtcggc
     1681 atccggcatc atagtccggc ctgcgcccgg ctggtgaaat cgttaatcga aagccagcgg
     1741 ccacgatacg tattgattga aggcccggct gattttaatg accgggtaga cgaactgttt
     1801 ttagcccacc agcttccggt agctatttac agttattgcc agtatcagga cggtgcagcc
     1861 cccgggcgtg gtgcctggac gccatttgct gaattttcgc cggagtggca ggcgctacaa
     1921 gccgcacgtc gtattcaggc acaaacttac ttcatcgatt tgccttgctg ggcgcagagt
     1981 gaagaagtgg acgattcgcc tgatacgcaa gaggaaagcc aggccttgct gctgcgtgcc
     2041 acccgcatgg ataacagcga caccctgtgg gatcacttgt tcgaagatga aagccagcaa
     2101 attgcattac cctctgcgct ggcgcactat tttgcccaac tgcggggcga tttccccggc
     2161 gatgcactca atcgtcagcg cgaagccttt atggctcgct ggattgcatg ggcggtgcag
     2221 cgaaataatg gcgacgtgtt agtcgtctgc ggtggctggc acgctcctgc actggcaaaa
     2281 atgtggcgcg aatgtccgca ggaaattaac aagccagaat tgccctcgct ggcaaatgcc
     2341 gttacaggtt gttatctcac cccctacagt gaaaagcacc ttgatgtgct ggcaggatac
     2401 ctttccggta tgcctgcccc ggtctggcaa aactggtgct ggcagtgggg cttacagcag
     2461 gccggtgaac aactgctaaa aacggttctc acccgtttgc gccagcacaa cttgcctgct
     2521 tcgatcgcgg atatggctgc cgcacatctg catgcaatgg cactggcaca gttgcgcggt
     2581 catacactac cgttacgcac tgactggctg gatgccatag caggctcgct gattaaagaa
     2641 gccctgaatg caccgttgcc gtggagctat cgcggcgtta ttcatcccga taccgatccg
     2701 attctgctaa cgttgataga cacattagcg ggtgacggat tcggtaaact tgccccttcc
     2761 acgccacaac cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac cgcaatctct
     2821 cttccggcgg agcttacctt aaatcgcttt acccccgatg ggctggcgca aagtcaggtg
     2881 ttacatcggc tggcaatact ggagatccct gggattgtac gccagcaggg aagtacactg
     2941 acacttgcag gcaacggtga agaacactgg aaattaaccc gcccgcttag ccagcatgcg
     3001 gcattgattg aggccgcatg ctttggtgca acacttcagg aagccgcacg ccataaatta
     3061 gaagccgata tgctggacgc gggtggcatc ggcagtatca ccacatgtct tagcctggcg
     3121 gcgttagcgg gtctggcgtc cttcagtcaa caattactgg agcaactcac attattaatc
     3181 gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg aagtgctata tgccttatgg
     3241 cggctggatg aaattagcgg tatgcaaggc gcgcagatat tacaaacgac gttatgcgcg
     3301 gctatcgatc gcacgctgtg gctgtgtgaa tctaacggca gaccggatga aaaggagttt
     3361 cacgctcacc tgcatagctg gcaagcgctt tgccatattc tgcgcgatct acatagcggc
     3421 gttaatttat ccggcgtttc gctttctgcg gcggtagcct tactggagcg acgcagtcag
     3481 gcgattcatg ccccggcgct ggatcgcggc gcggctcttg gcgcactaat gcgtctggaa
     3541 catcccaacg ccagtgccga agcggcgctg acgatgctgg cgcagttatc cccggcacaa
     3601 tccggcgagg cgctgcacgg tttgctggca ttagcccgtc atcaactggc ctgtcaacct
     3661 gcatttatcg ccggtttcag cagccatttg aatcaactga gtgatgccaa ttttatcaat
     3721 gccctgcccg atttacgcgc ggtgatggcc tggctaccgc cacgagaacg cgggacgctg
     3781 gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc ccgtttcggc actgcaaatg
     3841 ccgttgcatt gtccaccgca agccattgca catcatcaac aactcgaaca gcaggcactg
     3901 gcatcgctgc aacactgggg agttttccat gtctgaactg aacgatcttc tgaccacccg
     3961 tgagctacaa cgctggcgat taattcttgg cgaagcggca gaaacgacgc tttgtgggct
     4021 ggatgacaac gcccggcaga tagatcacgc gctggagtgg ctgtatgggc gcgatcctga
     4081 acggctccag cgtggtgaac gctctggtgg attaggtggc tcaaatctca ccactcctga
     4141 gtggttcaac agtatccaca cgctgtttcc acaacaggtg attgagcggc tggaaagcga
     4201 tgccgtactg cgctacggca ttgaagatgt ggtgacaaat ctcgacgtgc tggaacgtat
     4261 gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc aaacatctga tgaaccccga
     4321 agtactggct gccgcccgca ggattgtgcg ccaggttgtt gaagaaatta tggcgcgact
     4381 ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat cgccgtcgcc gctcatttat
     4441 tccactggcg cgaaactttg atttcaaaag tactctgcgc gccaacctgc aacactggca
     4501 cccgcaacac ggcaagttgt atatcgaatc cccccgcttt aacagccgca ttaaacgcca
     4561 aagcgaacaa tggcaactgg tcttactggt tgatcaaagc ggctcgatgg tcgattcggt
     4621 gatcccctct gcggtgatgg cggcctgttt gtggcagtta cccggcattc gtacccatct
     4681 ggtggcgttt gacacaagcg tcgttgatct cacggcagac gttgccgatc cggtagagtt
     4741 attaatgaaa gtacagttgg gcggcgggac caatatcgcc agtgccgtgg agtatggtcg
     4801 gcaacttatt gaacaaccag cgaaaagcgt cattatcctc gtgagcgatt tttacgaagg
     4861 gggttcatca tcattactga cgcatcaggt gaaaaagtgt gtccagagcg gcatcaaagt
     4921 gctgggactg gcagcgctcg atagcacagc aacaccttgc tatgaccgcg atacggccca
     4981 ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca ccgggcgagc tggcatcatg
     5041 gcttgcggag aatcttcagt catgaattca ctacgtccgg aattattaga actgacaccg
     5101 caggccctga cggcgttaag caatgccggt tttgttaagc gcagtcttaa ggaactggaa
     5161 aatggcaacg tcccggagat cagccatgag aacggcgctt taatcgccac cttcagtgac
     5221 ggtgtccgta cccagctggc gaacggccag gcactgaaag aggctcagtg cagctgcggg
     5281 gccagcggta tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg actttgtgct
     5341 accgcccagc ctacgggaaa agaagaggaa tgggatccgg caatctggct ggaagaactg
     5401 gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc tggtcgctaa aggcatcacc
     5461 attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc gcttaccgat gagcgatgtg
     5521 cgtttttatt cccgcagcag tattcgtttc gcccgttgtg attgtattga aggcacactt
     5581 tgcgaacatg tcgtactggc agtacaggcc tttgtccagg caaaagcgca gcaagcggaa
     5641 ttgactcatt taatctggca gatgcgcagc gaacacgtca catcatctga cgatccgttt
     5701 gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac aactaagcca ggcattatgg
     5761 ctgagcggca tcagccagcc gcttattcac tacgaggccg ctttcagccg cgcgcagcaa
     5821 gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc tacggcaact gcgcgccagc
     5881 gttgatgcct tccacgcccg cgccagccac tatcatgccg gagaatgctt acgtcagctt
     5941 gcggcattaa acagtcgatt aaactgcgca caagagatgg cccggcgcga cagtgttggt
     6001 gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg gcattgccgg agaagcaaag
     6061 cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat tgagcattat
     6121 ggtttacgca tctggtttac cgatcccgac accggcagta ttttgcatct ttcgcgtagt
     6181 tggccgcgaa gtgaacagga aaactcaccg gcagctacgc gtcggctgtt tagttttcag
     6241 gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag caaaacgcag tgccgatggc
     6301 gagctgctgt tagctacccg caaccgctta agcagcgttg tgccgctgtc gccagacgcc
     6361 tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg tggctttgcg ggaatattta
     6421 cgccagcgtc cccccgcctg catacggcct cttaatcagg tggataactt atttattctg
     6481 ccggtcgctg agtgtatttc actcggttgg gacagcggcc gccagacgct ggatgcgcag
     6541 gtaatcagcg gcgaaggtga agataatctg ctgacgttat cccttccggc atctgcctgt
     6601 tctccttttg ccgttgaacg catggcggcg cttttgcaac aaacagacga ccccgtgagt
     6661 ctggtttctg gctttgtcag ctttgtagag gggcaattga cactggaacc acgggtgatg
     6721 atgacaaaaa cccgtgcctg ggcgctggac gcggaaactg cgcctgtggc accgctacct
     6781 tctgccagcg ttttgcctgt gccgtctacc gctcatcagt tgctgatgcg ctgccaggcg
     6841 ttacttattc aactgctcca taacggctgg cgctatcagg aacagagcgc tattagtcag
     6901 gcagagttgc tggcgaatga cctcaccgcg gtgggttttt atcggctggc acatgtgttg
     6961 gtacaatttc gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgttttg
     7021 ctttgcgaac aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa
     7081 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     7141 ctggtgagag tcaccagttt cttgattaaa taaaatggag ttttacatga aggctttcaa
     7201 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     7261 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     7321 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     7381 cattagtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     7441 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     7501 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     7561 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     7621 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgt atggccgcca
     7681 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     7741 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     7801 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tagccagaat gtcatcggtt
     7861 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     7921 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     7981 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     8041 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     8101 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     8161 aggccttcag gtgttgatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     8221 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     8281 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     8341 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     8401 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     8461 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     8521 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     8581 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     8641 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     8701 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     8761 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     8821 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     8881 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     8941 ggtagttgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     9001 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     9061 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     9121 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     9181 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     9241 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     9301 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     9361 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     9421 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     9481 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     9541 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     9601 aataaacggt ttttggcttc atacaatttg atggtgccca tcacccgctg attttcacca
     9661 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     9721 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     9781 gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccagt aaaggccagc
     9841 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctgt
     9901 gccactttca tgctgttcac ttcgttaaac ccctggcgca aaatgccttc cgtcgaggca
     9961 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
    10021 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggtgca
    10081 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
    10141 aacatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
    10201 ttatcagtac gcccgcgacg aatcaggatg ctgtgtacca ggccgccgag caatccttca
    10261 acgatggtcg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaac
    10321 ccgccggtca ggccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
    10381 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
    10441 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
    10501 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
    10561 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
    10621 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
    10681 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
    10741 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
    10801 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
    10861 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttaa aaaatcgacg
    10921 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
    10981 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
    11041 aaacaatatc agaaattgat actggttatc catacagata aaaataacac ctgtacataa
    11101 ctacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
    11161 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa
    11221 cgttatgacg atgtagaggt gatcgtaaaa gccaccagca acgatggcct ttctgttaca
    11281 cgcaccgcag ataaggattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg
    11341 gagtctgctg acgagtggtt tgttcactaa taaacacgta aaatcggtaa cggctggaaa
    11401 tcattcaata ctcgcactat cgaaagttta ccagccaacc gcagcacgtc ctgcatacgt
    11461 cgtgtctgcg gtttttcttt ttcgcttaca ttgtgtctgg ttcttccggc cactcaatat
    11521 caggtgcagt tgatgtatca acacagttca gcaacacccg atacttcttc caggcttcca
    11581 gcaacgagtt ttcttcctcc tttgcgatct ccagatctac agcatcctga agtggcgcaa
    11641 tatgctcact gaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct
    11701 cctgctgtat cgaagcatac caggctattt caatatcgct atgctgcagc agcatttaac
    11761 cccttgtaat tcatcgccat aattgattta attcacaaat aaaactataa tatggtgaaa
    11821 ttaatgaaaa aaaacacaga tgatggggct aaaatttaca caccaattac cctaaagctt
    11881 tatgactggt gggttttggg agtatcaaat cggcttgcat ggggatgtcc tacaacggaa
    11941 caccttcttc cacactttct ggaacattta ggtaacaacc atctggatat tggtgttgga
    12001 actgggtttt accttactca cgtacctgag agtagtctga tatctttaat ggatttgaac
    12061 gaagctagcc tgaacgcggc atctacaagg gctggggaat caaaaattaa acataaaatt
    12121 agccatgatg tttttgaaac ttatcccgcg gcgttacatg gtcaatttga ttccatttcc
    12181 atgttttacc ttcttcactg cctgcctgga aatatatcta caaaaagctg tgtaatacgc
    12241 aatgcggcgc aggccttaac tgatgatgga actctatacg gagccacaat tcttggcgat
    12301 ggagttgtgc acaatagctt cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc
    12361 ttttcaaaca caaaagattc cgaagaaggc ttaacacata tactctcaga gcatttcgag
    12421 aatgttaaaa ccaaggttca aggtactgta gtaatgtttt ctgcttcagg gaaaaaatag
    12481 catccaaccg cagcacgttc ttgcttaaga cgtgctacgg tttcctttac gatttggggt
    12541 tgcgacttta cctctattga taatgcattc cggcagaaca ttcaaatatg aacgtacgat
    12601 atttaactaa ccgaaaaaca aaatatagca caggcgagac atttaccatc taagaaaata
    12661 gtatcgtttt tttacagtta aatcaacatc acttccttaa aatgaaaaca ataaatttca
    12721 atcctgaaac attttatttt agcttattat ttcccagtat cacattttat caggatatcc
    12781 ttctgcgagg ttattatgct tcctgtaaat aatccccccc tatccactgg aaacgtctct
    12841 ttttacagaa ctacatcaat cgacaatgtt cacaataatt atctctccga atgggttgaa
    12901 tggactaaaa acagcatttc cggagaaaac agggaaactg cttttacccg gctccaatta
    12961 tgtctggaga acagtgaaac atcgttggac ttatcttgtt taggtctcag atctctacca
    13021 cgattgcctg acaatcttga tgaaattaat gtaagcaata accaactatc aatgctcccc
    13081 gagctaccaa gggcattgaa agaactgaat gcaagcagta atcaattatc tgcacttcct
    13141 gaattaccag tgtcgctgga atatataaat gtgagtgata accatttgtt cgcacttcct
    13201 gaattacctg cgtcactaga atatattaac gtaagtgaca atcacctgtc tgtacttccg
    13261 aggttaccaa tgtcattgga attacttgat gcagccagaa atgctttgga agtaatacca
    13321 gattttccag aaagagatga tcatattata agaatattct ggcttaatca gaaccggatc
    13381 acggcaattc cggaaagcat acttggcctc agttctgata gcgttgtcaa tcttagagaa
    13441 aatcaactat ctcccagaat aatgcaaact ttgttacaac aaaccgccca
//
LOCUS       DADLTD010000111        13479 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLTD010000111 DADLTD010000000
VERSION     DADLTD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.111"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(102..3323)
                     /gene="carB"
                     /locus_tag="JN701_002550"
     CDS             complement(102..3323)
                     /gene="carB"
                     /locus_tag="JN701_002550"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY7364963.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3341..4489)
                     /locus_tag="JN701_002551"
     CDS             complement(3341..4489)
                     /locus_tag="JN701_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HAY7364964.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="JN701_002552"
     CDS             complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="JN701_002552"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY7364965.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="JN701_002553"
     CDS             complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="JN701_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY7364966.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="JN701_002554"
                     /gene_synonym="lytB"
     CDS             complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="JN701_002554"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY7364967.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="JN701_002555"
     CDS             complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="JN701_002555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7364968.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="JN701_002556"
     CDS             complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="JN701_002556"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY7364969.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="JN701_002557"
     CDS             complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="JN701_002557"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY7364970.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVLGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="JN701_002558"
     CDS             complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="JN701_002558"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY7364971.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12684..12902)
                     /locus_tag="JN701_002559"
     CDS             complement(12684..12902)
                     /locus_tag="JN701_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY7364972.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTVVQGILGRFSLF"
     gene            13005..13268
                     /gene="rpsT"
                     /locus_tag="JN701_002560"
     CDS             13005..13268
                     /gene="rpsT"
                     /locus_tag="JN701_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY7364973.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3149 a         3806 c         3397 g         3127 t
ORIGIN      
        1 aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta ttacttattc
       61 gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat ctgcgcgtgc
      121 atttcctgca ccgaaattac tttttcggtc gcgtcggcat tcagcgccat cgcggtagcg
      181 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
      241 acgcgggagt cttcaatcgc gcgacggcct gaagtggtgt tgatgatgta ggtatattcg
      301 ccattcttga tgcggtcctg aatgtgcgga cggccttcat gcaccttgtt caccagacgc
      361 ggattaatgc ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
      421 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
      481 gaaagcaacg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca
      541 aacgcttcag cgaaggtgcg gcccacgccc atcacttccc cggtggagcg catttctggc
      601 cctaacagcg ggtcaacacc cgggaattta ttgaacggca gcaccacttc tttcaccgag
      661 tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
      721 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacggta
      781 cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc
      841 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
      901 acatcctgaa tttcctgact taaggtgtag gctggcagag aacatgcgga gtcaccggag
      961 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
     1021 cagatggcat ccacgtcaac ttctaccgcg tcatcaagga agtgatccag caacactggc
     1081 gcatcgttag acacgctgac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
     1141 acgatttcca tcgcccgacc gccgagaacg taagacggac gtaccaccag cgggtagcca
     1201 atctctttcg ccttctctac cgccatttca atagcggtaa cggtggcgtt cgccggttgt
     1261 ttcagtttca gacgctcaac cgcatgctgg aagcgttcac ggtcttctgc acggtcgata
     1321 gcatccgggc tggtgccgat aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc
     1381 agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
     1441 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
     1501 gtggagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc
     1561 gccagcgagg cgtgtacaca gcagtagtcg aattcgatac cctgaccgat acggttcggg
     1621 ccgccgccga gaaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
     1681 tcataagtgg agtacatgta agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg
     1741 cgcttataaa ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc cgcttcgcgc
     1801 acgcccgcca gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
     1861 aattcagcgt tcaggccagt gatgcccact tccgctactt tctcttccag acgcaccagt
     1921 tcttcaatct gtaccaggaa ccagcggtca atattggtca ggttgaacac gccgtccaca
     1981 gacaggcccg cacggaaggc atcggcgatg taccagatac gctctgcgcc agcatctttc
     2041 agttcgcgac ggattttggt taacgcttcc gggtcatcga ggctcacttt cgggtcgaat
     2101 ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
     2161 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcacca
     2221 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
     2281 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc
     2341 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
     2401 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
     2461 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc
     2521 gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg
     2581 atggagtcac cggtatggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
     2641 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
     2701 tcatcaatca gcagctcttt ggttggggag agatccagac cgcgggcgca aatttcttca
     2761 aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
     2821 ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat cgtgtgtgcg
     2881 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
     2941 tcttctgctt tatcaatcgc atcggcggtc gcaccaatca tagtcacgcc gaactcttcc
     3001 aacacgccct gacgctccag ctccagagcg cagttcagcg ccgtctgacc gcccatcgtt
     3061 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtactacttc ccagtgaatc
     3121 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
     3181 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
     3241 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
     3301 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
     3361 actgctcaat taactcgata aagtggtcga acaacggcgc tgcgtcgtgt ggcccagggc
     3421 tggcttccgg gtgaccctgg aagctgaacg ccggtttatc agtgcgatga atgccctgta
     3481 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
     3541 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
     3601 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
     3661 gcgccagcag ctgatgaccg aggcagatgc cgaataccgg aatatcggtt tcgaggaatt
     3721 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
     3781 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
     3841 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
     3901 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg cctccggtca
     3961 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
     4021 ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccaacgcc gcatccgggt
     4081 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
     4141 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
     4201 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
     4261 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
     4321 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
     4381 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
     4441 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca
     4501 gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa
     4561 atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag
     4621 tcaacttaat ggcgggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
     4681 aagcgacaaa aagatcatat aacctgttct ggaaaacgct tgcacagcaa gaagattgca
     4741 tgaaataaaa gcgagcaggg aaaagtggac caaatggtca aattttacat taaaacaaca
     4801 aaaacagaag acaaaacatg taatttttgg cacttataga caatttaaaa acataacact
     4861 aaaaataaaa gggccaaaat taaagccctt tataatcaat aaattaaatg tgttattttt
     4921 gcaccataac aaatattttg tggttacaaa ctattgagat caagcacatc tcgcatatca
     4981 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcgcc gttagcaaat
     5041 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
     5101 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc
     5161 acacgttcac cggtgtggct ttcacgactg tagaccgcgc aatctttcag atctttatca
     5221 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
     5281 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
     5341 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
     5401 gcaatatcgg cggcagcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg
     5461 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
     5521 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc
     5581 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
     5641 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga
     5701 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
     5761 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
     5821 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
     5881 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacgacgc
     5941 cagagccagc acctcagcca cccactgctg gaagcctttc acatccagat ccaatgccac
     6001 ctttacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
     6061 aaattcgccc tgagtttcca ctgccacaaa acacggtttg agagtgaaca attccgggcg
     6121 caccagccag gcaatagcgc aaagatcgtg catacgcagt ccactttgca tactgccgct
     6181 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggcaaagt
     6241 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc tcatgacgat
     6301 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
     6361 ggcgtttggt gtgcagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
     6421 atacggttta cattccgggc attgtgaaag taacagcgca atattggtta acgggccgat
     6481 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
     6541 tggtatcccg agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
     6601 gccgtgcaca gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
     6661 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcgttgcggg tggttttctc
     6721 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat
     6781 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
     6841 atgcatgttt tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
     6901 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
     6961 acaatgtttt cttcacggcc ttccagcgga atggcttcac caccgcccag ctgctgcaaa
     7021 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
     7081 catttaacct ctttcaccca ctcttcctgg atgtctttcg catcgtcaat caaaaacgcg
     7141 cgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
     7201 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
     7261 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
     7321 agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag
     7381 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ccggcgattc gaccagatac
     7441 attccccctt ccgggttact gtactggccc attgtccctt ccacttccgg gtgcccggcg
     7501 tgaccgatga gaatagattc ttcgccacgg cgactggcgc gggcgacttc catatgcact
     7561 ttggtcacca gcggacaggt cgcgtcaaat accgtcaaat cgcgactttt tgcttcgtta
     7621 cgtaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg
     7681 ctaatctgct caataaagat agccccacgc tcacgcaagc tatcgaccac atagcggtta
     7741 tgtaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
     7801 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
     7861 atgttacgcc tccaatgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
     7921 cccggccaga ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
     7981 gccaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
     8041 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
     8101 aaacgccgca tctggctcca acgagaaggt ggttttatcg cccactttca gccccaacag
     8161 gtgttgctcc agcccttcag aaagagaagc atcacccagg cggaacagcg ccggtttacc
     8221 gttgttgcgg gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
     8281 gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttata tttgaggatt
     8341 tttgccggat gcggcgtaaa cgccttatcc ggcagggttt attattgttt tttcgctcta
     8401 gaaggcaaaa aaccttccag cacaatcagt gccgcaccga cacagatggc agtatcggca
     8461 aggttgaagg tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag
     8521 ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg
     8581 atattgttta gcttctgcgt ggccttcgag cgatacatca tcactgccag gatcacgcta
     8641 ataccaatcg caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa
     8701 ctaaacgccg cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc
     8761 gtatccccca gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc
     8821 acgactacca ccagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg
     8881 caaacttacg tttttcaccg tcaccggcga cgttgctgac acagcggccg cagatttctg
     8941 cgtgttccgc caccttgccg acatcctggg tgtagtgcca gcagcgtggg cacttctcac
     9001 cttcggcttt actcaacgcg actttcagcc ctttgagtac ttcgctctgc tgagcatcag
     9061 caggtgcgtc gtgatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt
     9121 catcgcccag cgcggtcagt ttcgccgcca gttccggttc tgcatacaag gttactgccg
     9181 cttccagcga gccacccact ttcttgtcgg cacgcgcttg ctcaatgact ttgttcactt
     9241 cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt catcgcttcg ctgtctgcca
     9301 ggccaaacag gccttcgtac cactcaccgg tgaagacgta tttttcacgt tcgcccggca
     9361 ggtagcccca cacttcatca gcggtgaagg agaggattgg tgccatccag cgcaccagcg
     9421 cttctgcgat gtgatacagc gcagtctggc agctacgacg cgccacactg tccgctttgg
     9481 cggtgtactg acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga
     9541 agcgcatcag acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt
     9601 cttcctgtgc cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg
     9661 gtttcaccat atcttttgct ggatcaaaac cgttcaggtt tgccagcagg aagcgcgcgg
     9721 tgttacggat acgacgatag ctatcggcag cacgtttcag gatctcgtca gaaacggcca
     9781 tttcaccggt gtagtcggtt gatgccaccc acagacgcag aatatccgcg cccagtttgt
     9841 tcatcacatc ctgcggcgaa acggtattgc cgatggattt agacatcttg cggccctgac
     9901 catccacggt aaagccgtgg gtcagtacct gacgatacgg cgctttgcct ttcatcgcgg
     9961 tggagatcat tagggaagac atgaaccaac cacggtgctg gtcagaacct tccagataca
    10021 tgtccgctgc gtgaccggca aattccggac gcacgtcaac aacagaagag tgggtagatc
    10081 cggagtcaaa ccatacatcc aatgtgtccg gcactttcac gtactgatca gcttcatcgc
    10141 cgaggatctc tttcgcatca agatcccacc acgcctggat gccgtcaact tcaacgcgtt
    10201 ttgccacttc ttccattagt tcgagagtac gcggatgcag ctcttccgtg tctttgtgca
    10261 cgaacagtga catcggtacg ccccaggtgc gctgacggga gatacaccag tcaggacggt
    10321 tagcaaccat cgactcgata cgcgcctggc cccagtccgg gatccactgc acgcctttga
    10381 tctctttcag tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggcg
    10441 tcgcgcggaa gatgatcggc gttttgtgac gccagcagca cggatagctg tgctgcattt
    10501 tctcaacgtg cagcagcgcg cctttttcct gcagcagcgc aacgacgatg tcgttcgctt
    10561 tgaagacgtt cacgccatcc agcgtcggat aagtgcccgg cagataagtg ccgtccgggc
    10621 caaccgggtt agcggtttcc aggccgtatt tctgaccgat cacatagtcg tccgggccgt
    10681 ggccaggcgc ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga tcgccgagga
    10741 ttgccggaac gtcgaagccc ataaacggat gggtaaagcg cagcaactca agctccgcac
    10801 cttttaccgt gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt acgctttcaa
    10861 ccagatcttt cgccagaatc acggcctgac cgtcgatctg caccagcgca tagtcgaaat
    10921 caggtgcaat agagattgcg cggttcgcag gcagagtcca cggcgtagtg gtccagatca
    10981 ccagcgagat tgggccgtta acgttgctta cggcaaattt tgctttcagt gcatcctgat
    11041 cgaccgcctg aaaagcaacg tcgatggacg gagaagtttt gtcgtaatac tcaacttccg
    11101 cttccgccag cgcagaacgg cagtcaacgc accagtgtac cggcttcgcg cctttgtgca
    11161 ggtgaccgtt gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt
    11221 ccatggtcag gtacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc
    11281 gttgaccgtc aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac tcggcggcgg
    11341 tgaatttctc acccggctta ccgtattctt gctctacttt cagctcgatc ggcagaccgt
    11401 ggcagtccca gccaagcaca tacggcgagt cataaccgga aagccctttg gacttcacga
    11461 taatgtcttt cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag
    11521 gagggccatc atgcagaatg aaggtttttt tgcctttttt agccgcacgg atgatgccgt
    11581 acagatcatc atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac
    11641 gcatcgggaa ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct
    11701 cggttccgtg tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa
    11761 attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgacgcaa
    11821 atcgctgctc attgcgtatt tttttacgca gcactacttg tatatggcga ccgtaaaggt
    11881 ccattgcaac atctaacaaa tgcacttcca gctgctggcg aataccggca accgttgggc
    11941 gtgttccgat gtttgccacg ccgggtaacg gcttttcacc gaggcccagc acttctacca
    12001 cataaacccc tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac
    12061 ctatagtgcg ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc
    12121 ccagtaaact ttctgccaga gccagattgt catccgccag cgcctgacgc acagcggtgc
    12181 tgctaatacg cacgccacct tcgcaaaaag tttgcgtact agtgatatcg aagccgtatt
    12241 ccatgccagc tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat
    12301 catcccctac ggcaagaaat tttacgcgca aatgcttcac cagaagatcg ctgatgaaat
    12361 tttgcgcggt taacgccgcg aaacgcctgt cgaaacgcac gcacagcacg taatcaacgc
    12421 cacactctgc aaggtaacgc agtttttccc gcagccgggt cagtcttgcc ggggctttat
    12481 cggtagcaaa cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc
    12541 gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt
    12601 cgaaattacc aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc
    12661 cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca
    12721 acggtcaagg ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgcc
    12781 atggcgcaaa tcacgggaag aaagtgaccg cctgctgcaa tttttatcgc ggaaaagctg
    12841 tattcacacc ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac
    12901 attaacggcg cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct
    12961 cggcctttga attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat
    13021 cagctaagaa gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct
    13081 ctatgatgcg tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg
    13141 ctgcacagaa agcatttaac gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc
    13201 tgatccacaa aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac
    13261 tggcttaatc gccaatttgc tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta
    13321 tgcctgctgc ttttatgatg cgtgagagaa cacactaccg gttgccagat agtcaaaaga
    13381 tgctggcttt tgcagacacg ccgttacact ttctgaactc ttcgtggctt aagaatcgta
    13441 gaaccatcgg ctgatacatt ctgaacaaga atatctact
//
LOCUS       DADLTD010000112        13370 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLTD010000112 DADLTD010000000
VERSION     DADLTD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.112"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(174..1097)
                     /locus_tag="JN701_002561"
     CDS             complement(174..1097)
                     /locus_tag="JN701_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7364974.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1200..2285
                     /gene="dmlA"
                     /locus_tag="JN701_002562"
     CDS             1200..2285
                     /gene="dmlA"
                     /locus_tag="JN701_002562"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HAY7364975.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2535..4145
                     /gene="yeaV"
                     /locus_tag="JN701_002563"
     CDS             2535..4145
                     /gene="yeaV"
                     /locus_tag="JN701_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HAY7364976.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4177..5301
                     /gene="yeaW"
                     /locus_tag="JN701_002564"
     CDS             4177..5301
                     /gene="yeaW"
                     /locus_tag="JN701_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HAY7364977.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5357..6322
                     /gene="yeaX"
                     /locus_tag="JN701_002565"
     CDS             5357..6322
                     /gene="yeaX"
                     /locus_tag="JN701_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY7364978.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6376..7491)
                     /gene="rnd"
                     /locus_tag="JN701_002566"
     CDS             complement(6376..7491)
                     /gene="rnd"
                     /locus_tag="JN701_002566"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY7364979.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7572..9257)
                     /gene="fadD"
                     /locus_tag="JN701_002567"
     CDS             complement(7572..9257)
                     /gene="fadD"
                     /locus_tag="JN701_002567"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY7364980.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9462..10043)
                     /gene="yeaY"
                     /locus_tag="JN701_002568"
     CDS             complement(9462..10043)
                     /gene="yeaY"
                     /locus_tag="JN701_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY7364981.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10083..10778)
                     /gene="tsaB"
                     /locus_tag="JN701_002569"
     CDS             complement(10083..10778)
                     /gene="tsaB"
                     /locus_tag="JN701_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY7364982.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10836..12746)
                     /locus_tag="JN701_002570"
     CDS             complement(10836..12746)
                     /locus_tag="JN701_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY7364983.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12878..13222
                     /locus_tag="JN701_002571"
     CDS             12878..13222
                     /locus_tag="JN701_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7364984.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3201 a         3556 c         3334 g         3279 t
ORIGIN      
        1 ccaaccagat aggtccagta attcagaacc ccgtattcag cgaacacatt aacctcttta
       61 attatctttc gatcatgcgc gattaaaggt gaatatgcta accaatctgt agcggcttag
      121 aaaggagaaa atcaggtttt aacccgatat caacccgata attgaatcat taatcaggca
      181 tgctccagtg aaaaattcgg gtagtgctcg gcaaaatact ggcgtaaaaa ttctaccgtt
      241 atccgcactt tcgctgacgt cgccagcctt gaaacataaa cggcccagac gttcgctggc
      301 tggtaatatt ccggtagcac ttgcactaaa tgaccactgg caatgttttc gctaacatcc
      361 caccaggaac gcagagcaat cccctgccca tcgagacacc actgatggac aatttctcca
      421 tgattagacg acagtgggcc ggtgacttta atcgcatggt ggccttcttt attgcgtaat
      481 tgccagacac caaaaggatg atcgcgctct ttaatgacca ggcacggcaa tgcagaaaga
      541 tcggttaaat gtttcggcgc gccatgttgc gcaataaatt ccggcgacgc gcagagaatg
      601 cggtaattgg tcgccagttt gcgggcgatt aaattagggg cgatgtcatc gccaatgcga
      661 atatcgagat cgacaccttc attgaccaaa tcgaccagcc ggtcttccac atcaaaacgt
      721 aattccagtt gcggatacgc cttcgccagc gctgataacg ccggagccac cacctgtcgt
      781 ccaaaaccaa agctgctgat aatacgcagc atcccctgtg gcacctgacg cacgtcagaa
      841 agttcgtcca tcatctgacc gacatcctgc aaaatccgct gcgcccattc ataaatccgc
      901 tctccttctt cggtaatggt gacgcggcgg gtggtgcggt gtaacaacac cacgtttagc
      961 gtttgctcca gcaaggcgat gcgcttgctg acgaacgccg gtgaaacgcc cagttcttcc
     1021 gccacggcgg caaaaccagc ccgacgagcc accagcataa agacgcgcaa atcattcagc
     1081 agcggtaaat tattcatgat tcgtgtttta tgtttcacca gttacgggga ttaattcctt
     1141 ttccgtcaat tataggatgg tgatgttgtc aattttgatg gtcaggaagt gagaacccaa
     1201 tgatgaaaac gatgcgtatt gctgcgatcc cgggagacgg gattggtaaa gaagtccttc
     1261 ctgaagggat tcgcgtgtta caggctgccg ctgagcgctg gggcttcgac ttgagttttg
     1321 agcaaatggg gtgggcgagc tgcgagtatt acagccatca cggtaaaatg atgccggacg
     1381 actggcatga gcaacttagc cgtttcgacg ccatctattt tggtgccgtc ggctggccgg
     1441 ataccgttcc ggaccatatt tcgttgtggg gttcgctgct gaaatttcgt cgtgaattcg
     1501 accagtacat caacctgcgc ccggttcgtc tctttcctgg cgttccctgc ccgctggcgg
     1561 gaaaacagcc tggcgacatc gatttttacg tggtcaggga aaacaccgaa ggcgaatatt
     1621 cctcgctcgg cggtagagtg aatgaaggta cagagcatga agtcgtcatt caggaatcgg
     1681 tatttacacg tcgtggtgtc gatcgcattt tgcgttatgc cttcgaactt gcgcaaagcc
     1741 gcccacgtaa gacgctaact tatgccacta aatcgaacgg tttagccatc agcatgccgt
     1801 actgggatga gcgagtggaa gcaatggccg agaattaccc ggagatccgc tgggacaagc
     1861 agcatattga tattctctgc gcgcgttttg tgatgcagcc ggaacgcttc gatgtggtgg
     1921 tggcgtccaa tttgtttggc gatatccttt ccgatcttgg cccggcctgc accggcacca
     1981 ttggcattgc cccatccgcc aacctgaatc cggaacgcac tttcccgtcg ctcttcgagc
     2041 ctgtccacgg ttccgcgccg gatatctacg ggaaaaatat tgctaaccct atcgccacga
     2101 tttgggccgg ggcaatgatg ctcgattttc tcggcaatgg cgatgagcgt ttccagcaag
     2161 cgcataacgg tattctggca gcaattgaag aagtgattgc tcacgggccg aaaacacctg
     2221 atatgaaagg caatgccacc acgccacagg ttgccgacgc gatttgcaaa attattttgc
     2281 gttaaggtca aaccagttta tttgaaccgc gtcactgacg cggtttttta ttcgttcttt
     2341 gcagtaaata acctgcgtca tttcaccttt tattgtttcc gtttcgtgtt ttatggcttt
     2401 ccgcattctt aattgtttaa tttatgtaat attcaaataa tcaatttgtt aaacacaaca
     2461 tacaactcat taccctgcaa ataattacaa ctggcaaaag ccagagacgg gcttttatgc
     2521 ccaggagatg actcatgatg agcaatgtta agaaaaaaga tgtgccgctg ataagcatca
     2581 gcctggtggc cattcttttc atcgcagctg cattaagcct tttcccacaa caatcggccg
     2641 acgcggccaa cgcaatatac acttttgtta cgcgtacgtt aggttccgcc gtacaggtat
     2701 tggttttgct ggcaatggga ctggtgattt atttagccac cagtaaatac ggcaatattc
     2761 gtcttggcga aggaaaaccg gaatacagca cgctctcctg gctgtttatg tttatttgtg
     2821 ccggtttagg ttcttctacg ctttattggg gggttgctga atgggcctat tattatcaaa
     2881 cacctggatt aaatatcgca ccgcgttcac aacaggcact cgaatttagc gttccctact
     2941 ctttcttcca ctggggcatc agcgcctggg caacttatac gctggcctca ttaatcatgg
     3001 cttatcactt tcatgtgcgg aaaaacaaag gtctgagcct ttccggcatt attgctgcta
     3061 ttaccggcgt tcgcccgcaa ggcccatggg gaaaactggt tgatttgatg ttcctgatcg
     3121 ccactgtcgg cgcactgact atttcccttg ttgtcaccgc agcaaccttt actcgtggac
     3181 tttccgcact gacaggttta cccgacaact tcaccgtgca ggcatttgtg atcctgcttt
     3241 ctggcggcat tttttgccta agctcgtgga ttggtatcaa caacggtttg caacgtctga
     3301 gcaaaatggt tggctggggc gcgttcctgc tgccattact ggtgctgatt gtcggcccaa
     3361 ccgaatttat taccaacagc atcatcaatg ccatcggcct gaccacgcaa aacttcctgc
     3421 aaatgagctt attcaccgat ccgcttggcg atggttcatt tacccgcaac tggaccgttt
     3481 tctactggct gtggtggatc tcatacaccc ctggcgtagc aatgtttgtc acccgcgttt
     3541 cccgcggtcg taagattaaa gaagttatct ggggactgat cctcggcagc accgtcggtt
     3601 gctggttctt cttcggcgta atggaaagct atgccattca tcagtttatc aatggcgtaa
     3661 tcaacgtccc acaggtgctg gaaacactgg gcggcgaaac agctgtacag caagttctga
     3721 tgtcgttgcc agccggtaaa ttgttcctcg ccgcatacct gggcgtgatg attattttcc
     3781 ttgcctcgca tatggatgca gtggcctaca ccatggcggc gaccagtacg cgtaatctcc
     3841 aggaaggtga cgatcctgac cgtgggctgc gtcttttctg gtgcgtggtg atcactctga
     3901 tcccgctttc catcttgttt accggtgctt cgctggaaac gatgaaaacc accgtcgtgc
     3961 tcacagccct tcccttcctc gtcattttac tggtgaaagt cggcggattt attcgctggc
     4021 tgaaacagga ttacgccgac attccggctc atcaagttga acattatctc ccgcagacac
     4081 cggttgaagc cctggaaaaa acaccagtga tccctgcggg aaccgtattc aaaggcgaca
     4141 actgagcgcc atcatcctaa cgataaaggt atccctatga gcaatctgag ccctgacttt
     4201 gtactacccg aaaatttttg cgctaacccg caagaggcgt ggaccattcc tgcccgtttt
     4261 tataccgatc agaacgcgtt tgaacacgaa aaagagaacg tcttcgccaa aagctggatt
     4321 tgcgtcgctc acagcagcga actggcgaat gccaatgatt atgtgacgcg tgagatcatt
     4381 ggcgaaagca tcgtgctggt acgcggtcgt gataaggttt tgcgcgcgtt ctataacgtg
     4441 tgtccgcacc gtggtcatca gttgttgagc ggtgaaggaa aagcaaaaaa tgtgattacc
     4501 tgcccgtatc acgcatgggc attcaaactc gatggcaatc tggcccatgc acgtaactgc
     4561 gaaaacgtcg ccaatttcga tagcgacaaa gcgcaactgg ttccggtgcg tctggaagaa
     4621 tatgccggat tcgtcttcat caacatggac cccaacgcca ccagcgtaga agatcagtta
     4681 cccggtctgg gcgcgaaagt gctggaagcc tgcccggaag tccacgatct gaaactggcg
     4741 gcccgcttta ccacccgcac gtctgccaac tggaagaaca ttgtcgataa ctatctcgag
     4801 tgctatcact gtggtccggc gcatccaggt ttctccgact ccgtacaggt tgatcgttac
     4861 tggcacacca tgcacggtaa ctggacgctg caatacggtt tcgccaaacc gtccgaacag
     4921 tcgtttaaat ttgaagaggg tacggatgcg gcattccacg gtttctggct gtggccgtgc
     4981 acgatgctga acgtcacccc gatcaaaggg atgatgacgg tcatttatga attcccggtg
     5041 gattctgaaa ctaccctgca aaactacgat atttacttca ccaatgaaga gttaaccgac
     5101 gagcaaaaat cgctgattga gtggtatcgc gatgtgttcc gtccggaaga tttacgtctg
     5161 gttgaaagcg tacagaaagg gctgaaatcg cgtggctatc gtggtcaggg gcgcatcatg
     5221 gccgacagta gcggtagtgg catttccgaa catggtatcg cccatttcca taatctgctg
     5281 gcgcaggtgt ttaaggacta atgacatcgg cggcggtatt ttccgctgct gggctgattt
     5341 ttgatggagt acagcaatgt cagactatca aatgtttgaa gtacaggtga gccaggttga
     5401 accccttacc gaacaggtga aacgcttcac gctggtggca accgatggca aaccgctgcc
     5461 tgcgttcacc ggaggaagtc acatcattgt gcagatgagc gatggtgata accagtacag
     5521 caatgcgtat tcactactga gttcgccgca tgacacctct tgttatcaga ttgccgttcg
     5581 gctggaggaa aactcgcgcg gcggttcccg ctttttgcat cagcaggtaa aagtgggcaa
     5641 tcggttaacg atttcaacgc ctaataacct gtttgcgcta attccctcag ccagaaagca
     5701 tctgtttatc gcgggcggta ttggtatcac ccctttcctg tcgcacatgg cagagctgca
     5761 acacagcgac gtcgactggc agctacatta ctgctcgcga aatccagaaa gttgcgcatt
     5821 tcgtgatgag ctagtccagc atccgcaggc tgagaaagtt cacttgcatc actcatcaac
     5881 cggaacacga ctggaattag cgcgattatt ggcggatatc gaacctggca cacacgttta
     5941 tacctgtggt cccgaggcgc taaatgaagc ggtaagaagt gaagctgcgc gtctggacat
     6001 cgccgccgat acgctgcact ttgagcaatt tgctatcgaa gacaaaaccg gcgatgcctt
     6061 taccctggtg cttgcccgtt ccggaaaaga gtttgtggtg ccggaagaga tgactatttt
     6121 gcaggttatt gaaaataata aagccgcgaa agtggaatgt ttatgtcgtg aaggggtatg
     6181 cggaacctgc gaaacagcaa tactggaagg tgaagctgac catcgggatc agtattttag
     6241 cgatgaagag cgtgccagcc agcaaagtat gttgatctgt tgttcgcgtg cgaagggtaa
     6301 acgcctggtg ttggatttgt agtttgccaa agccggatgt ggcgctgagc gcgcccagtc
     6361 cggcttcgga agattttact gcggatattc ctgcaataaa ttgtgtaatg cttccgccat
     6421 cagctcacca cgccagccgg aaatcagctc cggcaaattg ttctgcggtt tcagtttcca
     6481 gtgccagttc agcagttggt tgatttgccg acgcgatgcc agcaattcgg cgctgatctt
     6541 atgcgtttcg ctcacgtcag taatcagcga cttaatcgct ttaaacgctt tacgataacc
     6601 aggcatgtcc atcaggttaa gcatcggctg cggtaaggcc tcttccggca atgtctgcgc
     6661 tttttccacc agcgtcagca gcgttttacc gtgaaagcgg atttcgctac cggataaacc
     6721 caggctgtcc agttcgccta aacttcccgg catataacgc gcgaccgacc acaaatgctc
     6781 ttcacgcacg acaaagttca ccgccagatc acgctctcgc gccttgcgca gtcgccagtc
     6841 ggctaacagt tgcagacagg ccagttggcg tgtgcgtaac tgccaggcat tggtgatatc
     6901 acgccaggca tcttccggcg caacgacttc ctgacgacgc atttgcatca ggcggcattc
     6961 atccagcgcc gcaggtagcc agccggaggc ctccgtttct accataagct tggcggtgat
     7021 cggtaacaga taccagacat ccgccgctgc gtattcacac tgacgttcgg tcagcggtct
     7081 ggccagccag tcggtgcgcg attcactctt gtccagcgta acgccggaat actcttccac
     7141 catggaagcg aaaccccatg acatcgggcg tccgcagaag gcagaaagga tttgcgtgtc
     7201 aatcaagggt tgtggtaatt cgccaaagac attgaggaac acttccagat cttcactgcc
     7261 tgcatggaga aattttgtga tggacggatc gcgcaggatc gctttcagcg gtgaccagtc
     7321 ggtgatcccg agaggatcaa ttagcgccag atgctcgcca tcgaaaagtt gaatcaaccc
     7381 cagctgcggg taataagtgc gcgtacgaac aaattcagta tccagggcta tcgccggaaa
     7441 ggcacggacg gcttcacaca aagaagccag cgcatcgtcc gtggtaatca tttggtaatt
     7501 caaattgttt tctctttagt gggcgtcaaa aaaacgccgg attaaccggc gtctgacgac
     7561 tgacttaacg ctcaggcttt attgtccact ttgccgcgcg cttcgtcacg taattctcgt
     7621 cgcaaaattt ttccgacgtt agatttcggt aactcatcac gaaactccac cagcttcggt
     7681 actttgtatc ccgtgagctg acggcggcaa aaagtcacca gtgactcttc ggtaagcgat
     7741 ggatcttttt tcactacgaa gattttcacc gcttcaccac tggagccgga aggtacgcca
     7801 acagccgcga cttcctgtac gccaggatgc tgcatgacga catcttcaat ctcgttggga
     7861 tagacgttaa aaccggaaac cagaatcatg tcttttttac gatcgacaat gcgcaggaat
     7921 ccttcttcat ccattaccgc gatgtcgccg gtgtgtaacc agccattttt gatgatttca
     7981 tcggtagcat cgggacgctg ccagtaaccc agcatcacct gcggtccttt gacacaaagc
     8041 tcacccggtt gacctggtgg tacttcatta tcatcatcat ccaccagttt ggcttccgtc
     8101 gacggcaccg gcaaaccgat gctaccacta tgataatcaa tatcatatgg gttaacgctg
     8161 accagcggcg cacactcggt aaggccatag ccttccagca gatactgtcc ggtcagtttc
     8221 acccaacgct ctgccaccac ttgctgcact ggcatcccac cgcctgcgga aagatgcaga
     8281 ctggagaaat ccagctgctg gaactcttta ttgttcagca acgcattgaa caaggtgtta
     8341 acgccagtga tagcggtaaa cggatatttc gctaactctt ttaccaaccc tggaatatca
     8401 cgcgggttag tgataagcag gttctgccca cccagttcga taaacagcag gcagttaatg
     8461 gtcagggcaa aaatgtgata cagcggcagt gccgttacta ccagttcttt acctggatgc
     8521 agcaacggcc cataggtggc gttgacctgt tccaggttcg ccagcatatt gcggtgagtc
     8581 agcatcgcgc ctttcgccac acctgtggtg ccgccggtgt attgcaggaa ggctaaatct
     8641 tccggcacca gttcgggttt gacgtactgc atccggtagc cgttatgcag tgcgctacga
     8701 aatgaaatgg catctggcag atggtatttc ggcaccaaac gcttgatgta tttaacaacg
     8761 aaattgacta ccgtgccttt tgccgtagat agctgatcgc ccatacgggt cagaattacg
     8821 tgctgaacgg cggttttatc aaccactttt tccagtgtgt gagcaaagtt agacacgata
     8881 acaaccgccg atgcgccgct atcgttaagc tgatgctcaa gctcacgcgg ggtatacaac
     8941 gggttaacgt ttacgacgat catcccggca cgcaaaatgc caaacagcgc caccggatat
     9001 tgcaataaat taggcatcat caacgcaacg cgatcgcctt tcttcagccc caccccttgt
     9061 tgcaaataag cggcaaacgc gcgactgcgt tcttccagct tgcggaaggt catcacctcc
     9121 cccatattca caaacgcagg ctgatcggcg tagcgcgcga ccgactgctc aaacatatct
     9181 accagagatt gataacggtc agggttgatc tccgtcggaa cgtccgcggg ataacggtta
     9241 agccaaacct tcttcaattc ttcacctcta aaatgcgtgt tcgtcgtcat cgcaacccca
     9301 aatgatatac atgccgttaa cataatatta actcatcata ccagcttgat aattacccaa
     9361 cgaaaaggtt gcgaagcgcg tcactattta tttttatctt taccgtaaga atgcagaaac
     9421 agcggaccag ccgctgtttc tttttcttta aaaacaagca attattcagt tacaactgtt
     9481 tgtactctcg cgggaccggg attataccag ccccatccgc cgtagccata gggccagcca
     9541 cgaccgccat aaaaccatgg atcaatcggc tgaggcggca taatcacctg ctgggttaaa
     9601 tgccaacgtt tgtaacccgt tacttgcatc accataaatt tatagggcgt attgccgatt
     9661 ttgccgtcaa ccgcaccagt gattggcccg actaccgtaa ccagttgtcc acggaaatcc
     9721 accgggtcca gaaaaccgtt cacatcggca taaatgcgac cgcgagaagg ttctcccagc
     9781 gtcggtctgg ctccgctgtc cagcggtacg gtagcaattt ccaggcgggt tttcccttgc
     9841 tggttttgta ccgcaaccac tttgccacca aagcgtgcct cctgaccaac gtacagctgc
     9901 ggcgcactca tcacccgaac taaatcttgt tgcggcgtgg gactgcggcc tttaatggcg
     9961 tccggcacag tgacacaacc gctcagcatt agcgcaaacg tacctgccag tatgcctttg
    10021 ataacattct tttgaaccgc catgatgcga ctcctttttt tctcagagca tactcttaag
    10081 attcattctt tgcccggaag tttcttccat gcgacgttgt tacgtaaata aaccggttcg
    10141 gcatgttcca ccgccaccgt tttaccctcg gcaaatatct gacacgcaat cggcagcata
    10201 tcttccgtcg ctggcagtaa aacttctccg tcgcgcaaaa ccagcccgct ctctttaccg
    10261 agatccggcc aggcttgcca gcccgtccct accgttaccc attcgccgga aagctgttgc
    10321 attcgttcat gaacgagttc gggtttgagt acggcttcgg tttcttcacc gcgccagata
    10381 ccgttttcat cacgctgata ttcggcccag taaacttcgc ccatccgcgc atcaatggca
    10441 gccagcacgc gggttgcgcc gtttttgcgc cacgcccctt gcgccattgt cattagcgtg
    10501 gagacgccaa tcatcggcag ctctgcgcca agtgccagcc cttgcgcaat gccaatacca
    10561 atgcgcacgc cggtaaagct accggggccg cgcccgtaag ccagagcgtt aatatcagtc
    10621 agggaagttc cgctggtggt caggatatcc tgcaccatcg gtaagattcg ttgagtatgt
    10681 tcacgagggc aaagctcaaa atgagcgttg acagtaccgt cgttccacag ggcgacagag
    10741 caggcctctg tcgcggtatc gatagccaga attcgcatgg gtcttcgtgc ttagatcaat
    10801 aaaaaggcgc gcatcatacc atactccgta acaaattacc tggaggatgg tatcgcaagg
    10861 aaacgaaccg cacgggcaat gtcacgggtg cgcggcgcgg gcggcagact ggcgagaaac
    10921 gtcgcgccgt aagggcgcat caccagccga ttgtcacaaa tcaccaaaac cccgcgatcg
    10981 tcggcgtcgc gaatcagtcg ccctaccccc tgcttgagag taatcaccgc atccggcagt
    11041 tgtacttcat cgaacgggtc gccaccgcgc aaacggcaat cttccatgcg cgcttttaac
    11101 agcgggtcat ccggtgaggt aaacggcaac ttgtcgataa tcaccaatga caatgtatcg
    11161 ccacgcacgt ccaccccttc ccagaaacta ctggttgcca caagaagcgc gttaccggcg
    11221 ctgacaaatt gctgcaacag ttgccctttg ctggtttccc cctgcaacaa aacgggaagc
    11281 gtcatggtag cgcggaactg ctcggccaga tcacgcatca tggcgtgcga ggtacaaagc
    11341 ataaaacaac gaccgttgtt agcttcgatg atcggtcgca gcattgccgc taactggcga
    11401 gcagaacctg gctggttggt ttgcggcaga ttgcgcggca cacagagtaa cgcctggcgg
    11461 ctgtaatcaa acgggcttgg caatagcaac gactccgcct gttctatccc aagccgcgag
    11521 gtgaaatgat gcagatcgtc gttcaccgac agcgttgctg aggtgaagat ccagctacca
    11581 ggcttttgcg ccattaactc tttgaattta tccgccacgc tgagcggcgt gagagccaga
    11641 gtaaaatggc gcgaagtgca ttcgtaccag tagctgtagc ccggctgatt gatctctttt
    11701 agccgcttca gccgtgtgcg atacaacgta gcgcgctcaa atgccgcatc cagcaaggcg
    11761 gaacgcccca gtgacagttt cgccacgtca taacaaagtt ccagggtgtc atcgagcagt
    11821 aaaaatgccc gctgaatttg cggattagct aacagctcac gcaggttacc gcgataacct
    11881 ggctcaccga gttgcagacg aaaatcctgc gcactctggg caagacgatc agcgcacttt
    11941 tgtaactgct gggtgtcttt taattcggtg cggtaggcga tggtgatgtc ttttgccagg
    12001 tcgagcagtt gtcgactgga gagtgactga ccaaaatact ggctggcaat gtccggtagc
    12061 tggtgggctt cgtcgaagat catgacgtcc gcttccggga tcagttcgcc aaatccactc
    12121 tctttaacca ccatatccgc cagaaagaga tgatggttta ccaccaccac atcggcgtcc
    12181 atcgcttttt tacgtgcttt gaccacaaag caatctttat acatcgggca gtcgctgcca
    12241 agacagttgt cgttggtgct ggtaaccagc ggccacgcct gtgaatcttc cgccacgctg
    12301 acgcaggtgc tgatatcacc atcgactgtt tgattagacc aggagcgcag caggatcaca
    12361 tcgcttaaga tttgtaccgg cagatcgccc cccgccagcg cctgctgttc gagacgttcg
    12421 aggcagaggt agtttgagcg ccctttcagc agcgccacgt tgcccgtata tttcaatgcc
    12481 tttgagactg ttggcaaatc gcggctgtag agctgatcct gcaacgcttt tgagccggtc
    12541 gagataatga ctttcttttt cgcccgcagc gcaggagcca ggtaagcgta ggttttgccc
    12601 gtaccggttc ctgcttccac caccagcggc tggccttttt ctatcgcctg ggtgacggct
    12661 accgccatct gtcgctgtgg ttctcgcggc ttaaagcctg gtatcgcttt cgccagctga
    12721 ccgtctggtg caaaatcgtc cgtcacacta ccccctgttg atttgaacgg ggattatgtc
    12781 aggatgaggg cgctttcgcc agttgaagtg gtgacggcaa cctcacattg tggcagtctt
    12841 tgcagcacga aaatggaagt ataatgagga caaaatgatg actatcgttc gtatcgatgc
    12901 tgaagcccgc tggtctgatg tagtaatcca caacaacacg ctctactaca ctggtgtacc
    12961 ggaaaacctc gacgccgatg cttttgagca aaccgccaac acgctggcgc agattgacgc
    13021 cgtgctggaa aaacagggca gcaataaatc gagcattctg gatgccacca ttttcctggc
    13081 tgataaaaac gacttcgcgg cgatgaataa agcgtgggat gcctgggttg tggcgggtca
    13141 tgcgccggtg cgctgcacgg tgcaagcggg gctgatgaac ccgaagtata aagttgaaat
    13201 taagattgtg gctgcggtgt aagctttatc gaagcaaaat aagtcagacg ataatttatc
    13261 gataatactg gtcggtttta cataaatcga ccagagatta agatattact gttgatgctc
    13321 ctgctaattc attatttatt tttataattt caattttagc tataaagcta
//
LOCUS       DADLTD010000113        13357 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLTD010000113 DADLTD010000000
VERSION     DADLTD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.113"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..309
                     /locus_tag="JN701_002572"
     CDS             <1..309
                     /locus_tag="JN701_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364985.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="JN701_002573"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="JN701_002573"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY7364986.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="JN701_002574"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="JN701_002574"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY7364987.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3391
                     /gene="ilvY"
                     /locus_tag="JN701_002575"
     CDS             2501..3391
                     /gene="ilvY"
                     /locus_tag="JN701_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY7364988.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="JN701_002576"
     CDS             complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="JN701_002576"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY7364989.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="JN701_002577"
     CDS             complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="JN701_002577"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY7364990.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="JN701_002578"
     CDS             complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="JN701_002578"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY7364991.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="JN701_002579"
     CDS             complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="JN701_002579"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY7364992.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8059..9705)
                     /locus_tag="JN701_002580"
     CDS             complement(8059..9705)
                     /locus_tag="JN701_002580"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY7364993.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9708..9758)
                     /locus_tag="JN701_002581"
     CDS             complement(9708..9758)
                     /locus_tag="JN701_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7364994.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="JN701_002582"
     CDS             complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="JN701_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY7364995.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10294..11814
                     /locus_tag="JN701_002583"
                     /pseudo
     CDS             10294..11814
                     /locus_tag="JN701_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11839..12177)
                     /gene="maoP"
                     /locus_tag="JN701_002584"
     CDS             complement(11839..12177)
                     /gene="maoP"
                     /locus_tag="JN701_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY7364996.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12296..13135
                     /gene="hdfR"
                     /locus_tag="JN701_002585"
     CDS             12296..13135
                     /gene="hdfR"
                     /locus_tag="JN701_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY7364997.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13231..13306)
                     /locus_tag="JN701_002586"
     tRNA            complement(13231..13306)
                     /locus_tag="JN701_002586"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13271..13273),aa:Trp,seq:cca)
BASE COUNT         3096 a         3617 c         3467 g         3177 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 ggcctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc
      181 agtcgaaacc gattaaatag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catattgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactggc aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg ttcgataaag ttgtgttctc ttgtccggta
      721 ctggagccga ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag
     1081 aacagatagt taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac
     1201 tcttcaatga tgccggaatc gaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgca cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acgtcggagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca aatccgcctg cgggatcagt tcttcgtaag tgcccacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga tgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacattgag ggctgctcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtgacgct gactgaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca gttgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 accgattctc gaccgcttcc gcgcggaaca cccgtcggtg gagatcaaac taaccactgg
     2881 tgatgcggca gatgcgatgg aaaaggtagt cactggcgaa gcggatctgg cgattgccgg
     2941 gaaaccggag actctgcccg gcgcagtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcg cccgcgctgc cctgcccggt gcgtaaccag gtttcgtcag agaagccgga
     3061 ctggtctaca gtgccgttta tcatggccga tcagggaccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggcgtggcg ctgttgccgg aagtagtgct
     3241 ggaaaacagc cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gcgatgaaaa
     3301 aacgccgttt gaactgggcg tttgtgcgca gaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aagattttgc cgaaccatta acccgccaaa aagaacctga acgccggatt
     3421 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
     3481 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
     3541 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
     3601 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
     3661 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
     3721 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
     3781 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
     3841 gaccgctcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
     3901 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
     3961 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccagccgtt cgccgcgaat
     4021 gttgtgctgg gcgatatatt ttttcattcc cgccagcgcc agcgcgccag agggttccgc
     4081 caccgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
     4141 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcac cgatgcgttt
     4201 taccgctacg ccttcagcaa atagccctac gcgcggcaga tcaaccggat gacccgcatc
     4261 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
     4321 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
     4381 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
     4441 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
     4501 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
     4561 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
     4621 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
     4681 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
     4741 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
     4801 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgctg cctcgtaaac
     4861 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccag acaggggttg
     4921 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
     4981 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
     5041 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
     5101 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
     5161 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
     5221 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
     5281 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
     5341 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
     5401 accgcgtcgt cctggctttc gtacactttt gccgggccgg tgaatttgag gatgctgtca
     5461 tcgacgcccg cggttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc
     5521 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg
     5581 tcgtccagcg tatcccaacg gcaatcttgc gagaatgcct gggtggtacg gatacccgca
     5641 gggccggcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgc
     5701 tccagcgttt gcggcaacgt caggccgagc acgtttttca catcacggtt cagtaacccc
     5761 gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac atcttccata
     5821 tggtatttct gggtgctcgg cgcaacttta cacagctgtg gaaccttgcg ggaaagctta
     5881 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
     5941 acggtgttag tcgatccacc catcgcgata tccagcgtca tggcgttttc aaacgccgcc
     6001 ttactggcga tattacgcgg cagtgcactt tcgtcgtttt gctcgtaata acgtttggtc
     6061 aattcaacaa tgcgtttacc agcattaagg aacagctgct tacggtccgc gtgggttgcc
     6121 agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt cggtcaggca gttcattgag
     6181 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
     6241 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
     6301 agtttgatta tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca
     6361 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
     6421 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
     6481 tcagcgatca gttcgcgaga tggaagtgaa taaagcatcc ccccgtggcc catggcaatc
     6541 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
     6601 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
     6661 gagttcacaa ccgcgataat cggcttgcca aaatcggcgt cggtcattcc ggtggcgcgc
     6721 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
     6781 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat
     6841 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
     6901 gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccacaac ggccttcgcc
     6961 aacctgaata ccgtctacgc tgcgcactgg cgtgatttct gccgcagtac cggacataaa
     7021 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc
     7081 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggatgaggt
     7141 aaacggtggg gtgaacagca cgccgtcttt cacttcaaac aggttttcac ctgcgccttc
     7201 agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
     7261 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
     7321 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
     7381 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
     7441 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
     7501 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
     7561 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
     7621 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
     7681 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
     7741 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
     7801 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
     7861 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
     7921 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
     7981 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata
     8041 cattgacctg atattgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
     8101 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
     8161 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
     8221 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
     8281 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
     8341 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
     8401 cacattcatc atgaaagagc cgtcaccgga gatacagacg acggtatcgt tcggtcgcgc
     8461 gacttgtgcg ccaaccgccg ctggtaaacc aaaacccata gtgcctaagc cgctggaggt
     8521 gatgaaattt tccgggcgag tgtgggcgat gtgttgcgca gcccacatct ggtgctgccc
     8581 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
     8641 cggcgcgtag atagcgtcgc cgggatggtc gtaacgccag gcatgttcat cacgcagctg
     8701 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaattctg gtaacagagc
     8761 atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
     8821 gatatccata tggataacgc tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg
     8881 gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac tcctgcaccg cgaagttcgc
     8941 cgctttggtg ccatgcattc ccagcatgcc cagatagtac ggataatctg cttcaactgc
     9001 gcccagccct ttcagcgtgc aggtggcagg catttttgtg gtagcgagaa attctcgtaa
     9061 agcaggaact gcctgcgcca tacccacgcc accaccaacg tacagcatcg gtttttgcgc
     9121 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
     9181 aacggtggtg aaccacggtt ccaggtcacc gctggctagc tggatatctt ttgggatatc
     9241 gaccagaacc ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccataatgcg
     9301 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
     9361 caggacatcc acttcctgaa atgcatcagt gccgataaac ggtgcggaca cttgaccggt
     9421 gatggcaaca acagggatag aatctaacag tgcgtccgca agcccggtta tcaggttggt
     9481 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
     9541 gatagccgcc attgccgcac cctgctcatg tcggcacagc aagtgctcca cgccgccgtc
     9601 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
     9661 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtg
     9721 ctgaatgttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
     9781 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
     9841 atttctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
     9901 cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
     9961 cttgttcgaa ggaatgccta aagagttacc ataggttttg taaatagtac aagatatttt
    10021 ttggccgatt tttatgatgc gtttaataat ttcaatataa gttgttgttt tttaacaata
    10081 aaatagtgag taaaaaattc aatttcagta tgaatgttgt tcccttcctc gtagttcatc
    10141 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10201 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10261 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10321 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10381 cccggcttaa cgaaggtggg cttaccagaa acaacggtaa aagaagctcg cgatcgcgtg
    10441 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctg
    10501 gctccagctg atctgccaaa agaaggagga cgatatgatt tacctatcgc cattgcgttg
    10561 ctggcggcct cagaacagct tacagccaat aagttagatg aatatggatt agtcggagaa
    10621 ctggcgctta caggcgctct gcgtggcgtt cccggcgcaa tctccagtac aactgaagct
    10681 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10741 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10801 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10861 ctcagtgatg ttatcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10921 gggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    10981 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagtgc tgcgatatta
    11041 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11101 catcacagtg catcgttaac tgcgatggta ggcggtggcg caattccagg gcccggtgaa
    11161 atttcgctgg cgcataacgg cgtgcttttt cttgatgagt tacctgaatt tgaaaggcgt
    11221 acactggatg ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacgcga
    11281 gcaaaaataa cctatccagc ccgtttccag cttgtcgcgg cgatgaatcc cagccctacc
    11341 ggacattatc agggaaacca taaccgctgc acaccagagc aaacgctgcg ttatctcaac
    11401 cgactctctg gtccctttct cgaccgcttc gatctctcac tggagatccc attaccgcct
    11461 cccggtattt tgagtaaaac ggtagtgccg ggagaaagca gcgccaccgt taaacaacgc
    11521 gtaatggccg ccagagagcg ccaatttaag cggcagaata aactgaacgc ctggctggat
    11581 agtccggaaa tacgccaatt ctgcaaactt gagagcgaag atgcgatgtg gctggaagaa
    11641 acgctgatcc atctggggtt atcgattcgt gcctagcagc ggttattgaa agttgcacga
    11701 accattgctg atattgatca gtctgacatt atcacacgtc aacatttgca ggaggcagtt
    11761 agctatcgag cgattgaccg tttgctcatc catctgcaga aactactgac ataaaaaaag
    11821 ggcatttcgc cctttttatt aatcgtcaga atcggtgtag tcttcagcac cttcaacctg
    11881 cggtttaccg ccggaaaggg tgtgaaaacg ttttggacgc ttaatacgcg tcatatactt
    11941 ggaccacacg cgttctgctt ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa
    12001 gagtttctct tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata
    12061 accatgacgc tcaagcagtt gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc
    12121 acgtggataa tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct
    12181 gcacgctcct aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca
    12241 gagtgtaaaa caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga
    12301 tacggaattg ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcggc
    12361 tgaatcgctc tatctgaccc agtcagcagt gagctttcga atcagacaac tggaaaatca
    12421 actgggtgtg aaccttttca cccgccacag aaacaatatc cgtttaaccg ctgccggtga
    12481 aaaactactg ccttatgcag aaacgctcat gagcacctgg caggccgccc gcaaggaggt
    12541 ggcgcatacc tcacgacata acgagttttc tatcggtgcc agcgcctcat tgtgggaatg
    12601 tatgcttaat cagtggctgg gacgtttgta tcaaaatcag gatgcccata caggcttaca
    12661 gttcgaagcg cgaattgccc aacggcagtc tctggtaaaa cagctgcatg aacgccagct
    12721 tgatcttctt attaccactg aagcgcccaa aatggacgaa tttagtagtc agttgctggg
    12781 atatttcact ttagcgcttt ataccagtgc cccttcaaaa ctaaagggag atcttaatta
    12841 tctgcgactt gagtgggggc cagattttca acagcatgag gcaggtttga tcggtgctga
    12901 cgaagtgccc attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgatgct
    12961 taatggttgc acctggctac ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt
    13021 tgtcgatagc acaacacttt cacggccgct ttatgccata tggctgcaaa atagcgataa
    13081 aaatgcgttg attcgcgatc ttttgaaaat taacgtgctg gatgaagtgt attaatatga
    13141 atggctggca aggatgccgg tagaaggatt tacttcggag agggttattt cagataaaaa
    13201 aaatccttag ctttcgctaa ggatgatttc tggcaggggc ggagagactc gaactcccaa
    13261 cacccggttt tggagaccgg tgctctacca attgaactac gcccctaatt agggtggcgg
    13321 aacggacggg actcgaaccc gcgaccccct gcgtgac
//
LOCUS       DADLTD010000114        13218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLTD010000114 DADLTD010000000
VERSION     DADLTD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.114"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            158..505
                     /locus_tag="JN701_002587"
     CDS             158..505
                     /locus_tag="JN701_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY7364998.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            495..857
                     /locus_tag="JN701_002588"
     CDS             495..857
                     /locus_tag="JN701_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY7364999.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            854..1351
                     /locus_tag="JN701_002589"
     CDS             854..1351
                     /locus_tag="JN701_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7365000.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1359..2543)
                     /gene="emrD"
                     /locus_tag="JN701_002590"
     CDS             complement(1359..2543)
                     /gene="emrD"
                     /locus_tag="JN701_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY7365001.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIANMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(2962..3051)
                     /gene="tisB"
                     /locus_tag="JN701_002591"
     CDS             complement(2962..3051)
                     /gene="tisB"
                     /locus_tag="JN701_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY7365002.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(3339..3629)
                     /locus_tag="JN701_002592"
     CDS             complement(3339..3629)
                     /locus_tag="JN701_002592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365003.1"
                     /translation="MEVVIGDLVGFVFNNIYTSTALQGRQWKIFQQGKIEGLITFCTE
                     LQVTKNPAGARFHQPPAAGNRKAHCIMSTLKVYVFRAERAISARILTYLGHS"
     gene            3616..3714
                     /gene="ivbL"
                     /locus_tag="JN701_002593"
     CDS             3616..3714
                     /gene="ivbL"
                     /locus_tag="JN701_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY7365004.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            3820..5508
                     /gene="ilvB"
                     /locus_tag="JN701_002594"
     CDS             3820..5508
                     /gene="ilvB"
                     /locus_tag="JN701_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY7365005.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            5512..5802
                     /locus_tag="JN701_002595"
     CDS             5512..5802
                     /locus_tag="JN701_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY7365006.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            5876..6466
                     /gene="uhpA"
                     /locus_tag="JN701_002596"
     CDS             5876..6466
                     /gene="uhpA"
                     /locus_tag="JN701_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY7365007.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            6466..7968
                     /gene="uhpB"
                     /locus_tag="JN701_002597"
     CDS             6466..7968
                     /gene="uhpB"
                     /locus_tag="JN701_002597"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY7365008.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            7978..9297
                     /gene="uhpC"
                     /locus_tag="JN701_002598"
     CDS             7978..9297
                     /gene="uhpC"
                     /locus_tag="JN701_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY7365009.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            9435..10826
                     /gene="uhpT"
                     /locus_tag="JN701_002599"
     CDS             9435..10826
                     /gene="uhpT"
                     /locus_tag="JN701_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY7365010.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10871..12627)
                     /gene="adeD"
                     /locus_tag="JN701_002600"
                     /pseudo
     CDS             complement(10871..12627)
                     /gene="adeD"
                     /locus_tag="JN701_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            12802..>13218
                     /locus_tag="JN701_002601"
     CDS             12802..>13218
                     /locus_tag="JN701_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7365011.1"
                     /translation="MNNDNTDYVSNESGMLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMIS"
BASE COUNT         2936 a         3441 c         3677 g         3164 t
ORIGIN      
        1 cctcgtcctt cagggcgtgg aggatgtcaa tttgcgctat gatacccatc tgacctcctt
       61 gtagcggatg gcaaaagcat atcaggaaac gggcggacga aattcttaaa aaaaaccgct
      121 aaagatagag cgttagtgaa attgcaaagg agcaacaatg aaaatttccc gccttggaga
      181 agcgcccgat taccgcttct cgctggctaa tgagcgtact tttctggcgt ggatccgtac
      241 cgcactagga tttctggcgg cgggggttgg gcttgatcaa cttgcgccag atttcgccac
      301 gcccgtcatt cgtgaactgc tggcgttgtt gttgtgtctg ttttccggcg ggttagcaat
      361 gtatggctac ctgcgctggt tacgcaatga aaaagcgatg cgtctgaaag aggacctgcc
      421 ctacaccaac agcttattaa tcattagctt aatattgatg gtcgtggcgg tgatcgttat
      481 gggactggtg ttgtatgccg gatagccgca aagccagacg cattgccgac ccaggactgc
      541 agccggagcg cacatcactg gcgtggtttc gtaccatgct gggctacggc gcgttgatgg
      601 cgttggctat caaacacaac tggcaccagg cgggcatgtt attctggatt tcgattggca
      661 ttctcgccat cgtggcgctg atcctctggc actacacccg taatcgcaat ttaatggatg
      721 tcacgaatag cgatttttcc caatttcacg tagtccgtga caaatttttg atctccctcg
      781 cggtgttatc tctcgcaata ctgtttgctg taacgcatat acatcaactt atcgtattta
      841 ttgagagagt cgcatgacag gaagtcaggt cattgatgct gaagaggaca ggcataaact
      901 ggttgtagag tacaaggatg ctctgcaacc tgccgatttt tatcacaatt tcaaacagcg
      961 gggcatccgc tccgtacagc tcattcccta tcttgaattt gatgaccggg gcgatctgac
     1021 ggccgcttcg gtgacagcag agctgtgggg gaaattttta attgccctgt tcgagtgttg
     1081 ggtgcgggca gatatcagtc gtatctcgat tgagcttttt gacgccacct tacaaaaatg
     1141 gtgtggcggc gaaaacccgc aaccgcgccg tgactgccag gcgtgcgact ggcatcgcct
     1201 gtgcccgcac gctcggcagg agacgccaga cagcgtgctt tgtgcgggct atcaggcgtt
     1261 ttattcttac tccgcaccgc atatgcgggt gatgcgtgat ctgataaagc agcatcgttc
     1321 tcccatggag ctgatgacga tgctgcggtg acgtgcgctt aaacgggctg cccctgatgc
     1381 gacatccgcg tcgccagcgg cagccagcac agcacgatca acaatcccat taaggtcatc
     1441 aataacccca ggctaccctg accggtttgc ggcaacatcg cagagagcga cgccagcacg
     1501 ccggaaccaa tgttttgcag accgccgacc agcgcgccag ccgtgcccgc caggaagggg
     1561 aacggctcca tcgcgccgct ggtcgccagc ggaaacagca tcccggcacc gaaaaagaac
     1621 agcgcggcgg gaacgagcag cgtccagaca ttcatcacgc caaaccagtc ggggatccac
     1681 atcagcaagc cagccagcag gcagcagata accgactgcc acattaacgt ggagaagcgt
     1741 ttattgggac gtccggcaaa ccatgcgcca aaaaacgctg ccggaatcgg cagaataaac
     1801 aaaatactga ccgtcatact gctcagccct aacaccgcgc ccatcagcac gccggagcag
     1861 gcttcaaagg cggcaatccc ggccagaccg ccaatcagca tcagcaaata acagttaaaa
     1921 ccgctgttac cgaaaagcgt tttataactg gtaagcaggc gcgtgcgcgg tgcatcgacc
     1981 ggacgcgttt ccggcatcca gcgggccata ctgaaggtca caccaacaca aagaaccaac
     2041 aaaaagagat aacaggcgcg ccagttccac atcgtatcca gcagaccgcc gattagcggt
     2101 gcgagcaacg gactgacgag aatccccatg tttaacaggc tgttagcatg gcgcaactgt
     2161 gtccgttcat ataaatctcg cggcaaagta cgcgccatta cgccgccaac gccggtgccc
     2221 atcccctgca tcgcgctggc ggcaatcaat accgtcaaac tggaggtcgt gaccgcgacc
     2281 agcgttgcca gcataaaaat ggacattccg acgaggatca ccggtcggcg acccacacgg
     2341 tcggaaatcg ggccataaaa cagctgtgag acaccgtaag tcagcagata agcgcccatt
     2401 acgctctgca ccgccccttc acggacgttg agatcgcgcg ccatattggc aatagctgga
     2461 atataaatgg tttgcgccat ctgaccgacg gccacgagta ataccaacat caataacaaa
     2521 ttgacgtttc tttgcctttt cattatcacg gatgctttta taaaaataat tagaataagt
     2581 cactacgcat tcccataaat gcgggagagg aatctatcac attgatgaat gttagccaga
     2641 tatacgccag gaatggggaa ttgtttagcg gcagaatatg taaacaaaag cggcaataaa
     2701 tgttgccggg agggattacg tgcgaactgt aaccctctcc ccaaaggggc gagggggaaa
     2761 ccaccgttcc ctttcagaaa atgagctaaa cattgcacgg gacggtcccc tcgccccttt
     2821 ggggagaggg ctagggtgag gggcacaggc agcgcaggtg caaacattct gctggtagaa
     2881 gaagccccca actccaaccc gcacgctaaa tataaaaggg gagcggttac ccgctcccct
     2941 ttggtgcgac ttgaatctga attacttcag gtatttcaga acagcatcaa gcagttgcag
     3001 tgctgcaaca atgagtttga ggataagaat ggcgatatcc accaggctca tacgcgtctc
     3061 ctgtggttca ggagcactgc cagctgacgt acctttccgc tgcctgttgc cagctgttgt
     3121 gcacgcggta tctcaaggag aaacgttgtg cttgttgaca taacacagtg tgctcacgcg
     3181 gggttaaggc gctgacggca ccacccgttt cagccaggac ttagtgcgcc gggtaacgat
     3241 gcggcccccg cataatacat tgcgtacagt gatattatag aagtttactg tataaataaa
     3301 cagtgatatt tggacaaaac gcaaactgtg atcgatactc aactatgccc taaatacgtc
     3361 aaaattcgtg ccgaaattgc gcgttctgcg cggaacacgt atactttcag tgttgacata
     3421 atacagtgtg ctttgcggtt accagctgca ggcggctgat gaaacctcgc tccggcgggg
     3481 ttttttgtta cctgcaattc agtacaaaac gtgatcagcc cctcaatttt cccttgctga
     3541 aaaattttcc attgtctccc ctgtaaagct gtgcttgtat aaatattgtt aaacacaaaa
     3601 ccaacaaggt ccccaatgac tacttccatg ttcaacgcaa aactactacc aactgcgcta
     3661 tccgccgcag tggtcgtcgt gcgtgtggtg gtggtcgtcg gcaatgcgcc gtagggactg
     3721 gaacaacaca cgattccaaa accccgccgg cgcaaaccgg gcggggtttt tcgtttaagc
     3781 acctcccgga aagtcggccc agaagaaaag gactggagca tggcaagttc gggcacaaca
     3841 tcgacgcgta agcgctttac cggcgcagaa tttatcgttc atttcctgga acagcagggc
     3901 attaagattg tgacgggcat tccgggcggt tctatcctgc ctgtttacga tgccttaagc
     3961 caaagcacgc aaatccgcca tattctggcc cgtcatgaac agggcgcggg atttatcgct
     4021 caggggatgg cgcgcaccga cggtaaaccg gcggtctgta tggcctgtag cggaccgggt
     4081 gcgactaacc tggtgaccgc cattgccgat gcgcggctgg actccatccc gctgatttgc
     4141 atcaccggtc aggttcccgc ctcgatgatc ggcaccgacg ccttccagga agtggacacc
     4201 tacggcatct ctatccccat caccaaacac aactatctgg tcagacatat cgaagaactc
     4261 ccgcaggtca tgagcgatgc cttccgcatt gcgcaatcag gccgcccagg cccggtgtgg
     4321 atagacattc ctaaggatgt gcaaacggca gtttttgaga ttgaaacaca gcccgctatg
     4381 gcagaaaaag ccgccgcccc cgcctttagt gaagaaagca ttcgtgacgc agcggcgatg
     4441 attaacgctg ccaaacgccc ggtgctttat ctgggcggcg gtgtgatcaa tgcgcccgca
     4501 cgggtgcgtg aactggcgga gaaagcgcaa ctgcctacca ccatgacttt aatggcgctg
     4561 ggcatgttgc caaaagcgca tccgttgtcg ctgggtatgc tggggatgca cggcgtgcgc
     4621 agcaccaact atattttgca ggaggcggat ttgttgatag tgctcggtgc gcgttttgat
     4681 gaccgggcga ttggcaaaac cgagcagttc tgtccgaatg ccaaaattat tcatgtcgat
     4741 atcgatcgcg cagagctggg taaaatcaag cagccgcatg tggcgattca ggcggatgtt
     4801 gatgacgtgc tggcgcaatt gatcccgcag gtggaagcgc aaccgcgtgc agagtggcac
     4861 cagttggtag cggatttgca gcgcgaattc ccgtgtccaa tcccgaaagc gtgcgatcca
     4921 ttaagccatt acggcctgat caacgccgtt gccgcctgtg tcgatgacaa tgcgattatc
     4981 accaccgatg tgggtcagca tcagatgtgg accgcgcaag cttatccgct caatcgccca
     5041 cgccagtggc tgacctccgg tgggctgggc acgatgggtt ttggcctgcc tgcggcgatt
     5101 ggcgcggcgc tggcgaaccc ggatcgcaaa gtgttgtgtt tctccggcga cggcagcctg
     5161 atgatgaata ttcaggagat ggcgaccgcc agtgaaaatc agctggatgt caaaatcatt
     5221 ctgatgaaca acgaagcgct ggggctggtg catcagcaac agagtctgtt ctacgagcaa
     5281 ggcgtttttg ccgccaccta tccgggcaaa atcaacttta tgcagattgc cgccggattc
     5341 ggcctcgaaa cctgtgattt gaataacgaa gccgatccgc aggctgcatt gcaggaaatc
     5401 atcaatcgcc ctggcccggc gctgatccat gtgcgcattg atgccgaaga aaaagtttac
     5461 ccgatggtgc cgccaggtgc ggcgaatact gaaatggtgg gggaataagc catgcaaaac
     5521 acaactcata acaacgtaat tctggagctc accgttcgca accatccggg cgtaatgacc
     5581 cacgtttgtg gcctttttgc ccgccgcgct tttaacgttg aaggcattct ttgtctgccg
     5641 attcaggaca gcgacaaaag ccatatctgg ctactggtca atgacgacca gcgtctggag
     5701 cagatgataa gccaaatcga taagctggaa gatgttgtga aagtgcagcg taatcagtcc
     5761 gatccgacga tgtttaacaa gatcgcggtg ttttttcagt aaccgctcaa ggcttgaaca
     5821 acatcgcgct tatcgttaag gtaagcgcgt tttttttacc cgccaggaca agaccatgat
     5881 caccgttgcc cttatagacg atcacctcat cgtccgctcc ggctttgcgc agctgctggg
     5941 gctggaacct gatttgcagg tagttgccga gtttggttcg gggcgcgagg cgctggcggg
     6001 gctgccgggg cgcggtgtgc aggtgtgtat ttgcgatatc tccatgcccg atatctccgg
     6061 tctggagctg ctaagccagc tgccgaaagg tatggcgacg attatgctct ccgttcacga
     6121 cagtcctgcg ctggttgagc aggcgcttaa cgcgggggca cgcggctttc tttccaaacg
     6181 ctgtagcccg gatgaactca ttgctgcggt gcatacggtt gccacgggcg gctgttatct
     6241 gacgccggat attgccatta aactggcatc cggtcgtcag gacccgctaa ccaaacgtga
     6301 acgccaggtg gcggaaaaac tggcgcaagg aatggcggtg aaagagattg ccgccgaact
     6361 gggcttgtca ccgaaaacgg tacacgtcca tcgcgccaat ctgatggaaa aactgggcgt
     6421 cagtaacgac gtagagctgg cgcgccgcat gtttgatggc tggtgatgaa gacgttgttc
     6481 tcccgcttaa ttaccgttat tgcctgcttt tttatcttct ctgccgcatg gttttgcctg
     6541 tggagtatca gcctgcatct ggttgagcgc cctgatatgg cagtgctgtt atttccgttt
     6601 ggtctgcgtc tggggctaat gctgcaatgc ccgcgcggat actggccggt gctgctgggc
     6661 gcggagtggc tgctgattta ctggctaacg caggcggtcg gtttaaccca ttttccgtta
     6721 ttgatgatcg gtagtttact gacgttactg cccgtagcgc tgatctcgcg ctatcgccat
     6781 cagcgtgact ggcgcacctt gctgttacag ggggcggcgt taacggcggc ggcgttgttg
     6841 cagtcgctgc cctggctttg gcacggcaaa gagtcgtgga atgcgctgtt gctgacttta
     6901 actggcggcc tgacgctggc cccgatatgt ctggtgttct ggcactatct cgccaataac
     6961 acctggctgc cgctcggccc gtcactggtt tctcagccaa tcaactggcg cgggcgacat
     7021 ctggtctggt acttgctgct gtttgttatc agtctctggc tccagttggg attgccggac
     7081 gaactgtcgc gctttacgcc attctgtctg gcgctgccga ttatcgcgct ggcctggcac
     7141 tatggttggc aaggggcgct gattgcgacg ttgatgaacg ccatcgcgct gatcgccagt
     7201 caaacctggc gcgatcatcc ggtggattta ttgctctcgc tgctggtgca aagtctgaca
     7261 gggttgttgc ttggcgctgg catccagcgg ttgcgtgaac ttaaccagtc gctgcaaaag
     7321 gaactggcgc gcaatcagca tctggctgaa cggttgctgg aaaccgaaga gagcgtgcgc
     7381 cgtgatgtgg cgcgtgagct gcatgatgat atcggtcaga ccatcactgc tattcgtact
     7441 caggcgggca ttgttcagcg gctggcggca gataacgcca gcgtgaagca gagcgggcag
     7501 ctcatcgaac aactatcgct gggcgtttac gacgcggtgc gccgtttgtt gggtcggtta
     7561 cgtccgcgcc agttggatga tctcaccctg gagcaggcca tccgctcact gatgcgggaa
     7621 atggagctgg aagggcgcgg tattgtcagc catctcgaat ggcgaatcga tgaatcagcg
     7681 ttaagcgaaa accagcgcgt gacgctgttt cgtgtctgcc aggaagggct gaacaacatt
     7741 gtgaaacatg ctgatgccag cgcggtcacc ctgcaaggct ggcagcagga tgaacggttg
     7801 atgctggtta ttgaagacga tggcagcggt ttgccgccgg gttccgggca acaaggtttt
     7861 ggcctcaccg gaatgcgcga gcgcgtaacg gcgctgggtg gcacattaca catttcctgt
     7921 ctgcacggca cgcgtgtcag cgtttctcta cctcaacgct atgtctaagg tttgatgatg
     7981 ttgccgtttc tgaaagcgcc tgccgatgcg ccattaatga ctgataaata tgaaattgat
     8041 gcccgctatc gctactggcg tcggcatatt ctgcttacca tctggctggg ttacgcgctg
     8101 ttttacttca cgcggaaaag ttttaacgcc gccgtaccag aaatccttgc taacggcgtg
     8161 ctcagccgta gcgatatcgg cctgttagcg accctgtttt acattaccta tggcgtgtcg
     8221 aagtttgtct ccggcattgt cagcgatcgc tcaaatgccc gttattttat ggggataggg
     8281 cttatcgcca cgggcattat caacattctg tttggcttct cgacgtcgct atgggcgttt
     8341 gccgtactct gggtgctgaa cgcctttttc cagggctggg gttcaccggt gtgtgcgcgt
     8401 ctgttaacgg cctggtattc acgtaccgag cgcggtggtt ggtgggcatt atggaacacg
     8461 gcgcataacg tcggcggcgc actcattccc attgtgatgg cagcggctgc actacattac
     8521 ggctggcgtg ccgggatgat gattgctggt tgtatggcga tcgtcgtggg aatttttctc
     8581 tgctggcggc tgcgcgatcg cccgcaggcg ttaggtttac cggcggtcgg tgaatggcga
     8641 cacgacgcgc tggagatagc tcaacaacaa gaaggggcag ggctgacgcg taaagagatc
     8701 ctcaccaaat atgtgttgct gaatccgtat atctggctgc tttcgttttg ctatgtgctg
     8761 gtctatgtgg tccgggcggc gatcaacgac tggggcaatt tgtatatgtc cgagacgctg
     8821 ggcgtcgatc tggtcacggc gaatacggca gtgacgatgt ttgaactggg tggatttatc
     8881 ggtgcgctgg tagcgggttg gggctcggac aaattgttta acggcaaccg agggccgatg
     8941 aatttgattt tcgccgccgg aattttgctt tcagtcggct ccctgtggct gatgccattt
     9001 gccagctacg tgatgcaggc aacctgcttc ttcaccattg gtttttttgt ctttggccca
     9061 cagatgttaa tcggtatggc ggcggcagag tgttcccaca aagaggcggc aggggcggcg
     9121 acggggtttg tcggcttgtt tgcttatctg ggggcgtcgc ttgctggttg gccgctggcg
     9181 aaagtactcg atacctggca ctggagcgga ttttttgtgg ttatctctat cgccgccggg
     9241 atttccgcac tgctgttact gccctttttg aacgcccaga caccgcgcga agcgtgatgc
     9301 atctcacctt ttcacttcat atccggcaaa actaagaaat tttccaggtt ttgcctggac
     9361 gctatctcag gcctgatttg ctgctgattt ttacaatgca tgcctcacgc aggtattcat
     9421 ttcaggagta acccatgctg gctttcctaa accaggttcg caagccgacc ctggaccttc
     9481 cgctcgaagt gcggcgcaaa atgtggttca aaccgttcat gcaatcctac ctggtggtct
     9541 ttatcggcta cctgacgatg tacctgattc gcaagaactt taacatcgcg cagaacgata
     9601 tgatttcgac ctacgggttg agcatgacgc agctggggat gatcggcctg ggtttctcca
     9661 tcacttatgg cgtgggtaaa acgttggttt catactacgc cgacggcaaa aacaccaaac
     9721 aattcctgcc gttcatgctg atcctgtctg ccatttgtat gctgggcttc agtgccagta
     9781 tgggcagcgg ctcggttagc ctgttcctga tgattgcctt ctacgcctta agcggctttt
     9841 tccagagtac cggcggttcg tgcagttact ccaccatcac caaatggacg ccgcgtcgta
     9901 aacgcgggac attcctcggt ttctggaata tttctcacaa ccttggcggt gcaggcgcag
     9961 caggtgtggc gctgttcggg gcaaattacc tgttcgatgg ccatgtcatc ggcatgttta
    10021 tcttcccgtc gattatcgcg ctgattgtcg gttttatcgg cctgcgttac ggcagcgact
    10081 ccccagaatc ttatggcctc ggcaaagctg aagaactgtt cggcgaggag atcagcgaag
    10141 aggacaaaga gacagaatct accgatatga ccaagtggca gatctttgtt gagtatgtgc
    10201 tgaaaaacaa agtgatctgg ctgctgtgct tcgccaacat tttcctctat gtggtacgta
    10261 ttggtatcga ccagtggtca accgtatacg cgttccagga actgaaactc tctaaagcgg
    10321 tggcgattca gggctttacg ctgtttgaag ctggtgcgct ggtcggtacg ctgctgtggg
    10381 gctggctctc tgacctggcg aacggtcgcc gtggcctggt ggcctgcatc gcgctggcgc
    10441 tgattatcgc cacgctcggt gtgtatcaac atgccagcaa cgaatatatc tatctggctt
    10501 ctctctttgc gttgggtttc ctggtctttg gcccgcaatt gttgattggt gtggctgctg
    10561 ttggctttgt acctaaaaaa gcgattggcg ctgccgatgg tattaaaggc acctttgctt
    10621 acctgattgg tgacagcttt gccaagttag gtctgggaat gattgccgat gggacgccgg
    10681 tattcggcct taccggctgg gcaggcacct tcgccgcact tgatatcgcc gcgattggtt
    10741 gtatctgcct gatggcgata gtggcggtga tggaagaacg caaaatccgc cgcgagaaaa
    10801 aaattcagca gttgacagtg gcataaacgt aactggtgac ttttgcccgg catgacgccg
    10861 ggctttttta ttattccgtg acttccagcg tagtgaagac aaacttctcg ccatcaaata
    10921 gcccctgact ggttagtttg agcgcgggga tcactggcag agaaagaaac gccatctgaa
    10981 taaacggctc atcgggtaac ggaccgcatt cacgggcggc ggctttcaag gcgtcaattt
    11041 gttccgccag cgactgcgcc gtgtcggtgc tcatcaggcc ggcaatgggc aacggcagat
    11101 gactttggac ctggccgtta cgtaccacgc acagcccgcc gccatcctga atcacctgat
    11161 agaccgccag cgccatctct tcggcactgc gaccgatcac cacaatatta tggctgtcat
    11221 ggctgaccgt cgcagccagc gcaccttcat tcagtccaaa gccgccaagc aaaccacaag
    11281 ccggagccag ccgttgcccg taacgctcaa gtacggcaat aaagcacaca tcatcgcgat
    11341 caaaaccatt ttcgctgtag acgctggagt gggagtgcgt aatcaattcg ttatggatga
    11401 cgtcaatgac ccgatagcgt tttccgggcg taaattgcag ggcaaagtcg ctggcggaaa
    11461 ctggctggcg ggcaatggtg ttgccatatg gcggagcgga ttgtgccagt ctcgccgact
    11521 cttccgcctg taaggtttgc gctcgccttt caccagtacc tgctgcaccg tgaccttacg
    11581 cgcatcgctc aacaggacga tatcggcctg cttgccgggt gccagtaagt cgaggtgatt
    11641 cagaccaaag tggcgcgccg tcgaccagct ggcgacgcga tatgccacat gcagcggcac
    11701 attgtgttgt tcgatcaggc ggcgaattaa ggcatcgatg tgtccttcat gggcgatctc
    11761 ccacgggtta cggtcatcgg tacagagcat gcattgcggg ctgttaaatt cgttgatcaa
    11821 cggtgccagc gcgttgagat tgcgggcagc ggacccttcg cggatcatca acgacatgcc
    11881 gagttgtaat ttccggcgtc cttcttccag ctgataactt tcgtggcagt tttcaatacc
    11941 cgcagtaata taggcgttaa gttctttacc acccaaaccc gggcagtgac cgtccagcgt
    12001 caggtggcga aatgcatcca gtttatcgag cagcgcattc tgcccgctaa ttacgccagg
    12061 gtagtccatc atttctgcaa ggccggtaac ctgcggatgg tcccgccagg cgagcatctg
    12121 ttcaagggta aaactggcac cgttaacatc gcagccttcc agggcgggta cgcaagagct
    12181 gacctgtaag tactggtttt gccttgcctg ttcggcacag cgggcaaacc aggcgaatcc
    12241 ggcttctccc atcacgttga cgatttcatg agggtcgcaa ataacggtcg tcaggccgcg
    12301 cggcagggta gcggtttcaa aggtgaccgg cgtcatcatg ctggattcaa tatgcaggtg
    12361 agcatcaata aaccctggca ccgccgttgc gccgcgagca tcaatccgct gcaaagccgg
    12421 agcatcagcg tattctgcgc caacaccggc aatgtaacgt cctttaatca caattgggcc
    12481 ggaaatttct ccgccattga tcaggtcgag aatagagaca ttatcaataa tataatcggc
    12541 aacagcgtcg ccacgggaaa cggctaacaa ttcctggtat tcagcccggc taatgtgatg
    12601 aaatttatgg ttaatggaat tattcatatt tttctcattt tgattttatc aatttgatta
    12661 ttatttatca taaagaaacg tcgcgagatt attaaagaat atctattaat gtgcaattga
    12721 aatgtgataa ttatcactga accgttgttg tcatttgttt tacaaaagca agggattaaa
    12781 tctcctccaa tggacaaaaa aatgaataat gacaataccg attacgtgag taatgaatca
    12841 gggatgcttt cgcgattatt taaactacct cagcatggga ccaccgtccg cacagaattg
    12901 attgcgggga tgaccacttt tttaaccatg gtgtacatcg tttttgtgaa cccgcaaatc
    12961 ctcggcgcgg cacaaatgga cccgaaagtg gtgtttgtta ccacctgttt gattgccggt
    13021 atcggcagta ttgcgatggg gatatttgct aacttacccg tggcgctggc tccggcaatg
    13081 gggctgaacg ccttctttgc cttcgtggtc gtgggggcga tgggcatctc ctggcagacc
    13141 gggatgggcg caatattctg gggcgcagtt ggactatttt tgctcacgct gtttcgtatc
    13201 cggtactgga tgatctcc
//
LOCUS       DADLTD010000115        13096 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLTD010000115 DADLTD010000000
VERSION     DADLTD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.115"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            176..1150
                     /gene="cysB"
                     /locus_tag="JN701_002602"
     CDS             176..1150
                     /gene="cysB"
                     /locus_tag="JN701_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY7365012.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1445..1609
                     /locus_tag="JN701_002603"
     CDS             1445..1609
                     /locus_tag="JN701_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY7365013.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1612..1779
                     /locus_tag="JN701_002604"
     CDS             1612..1779
                     /locus_tag="JN701_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365014.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1893..1988
                     /locus_tag="JN701_002605"
     CDS             1893..1988
                     /locus_tag="JN701_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365015.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2152..4827
                     /gene="acnA"
                     /locus_tag="JN701_002606"
                     /pseudo
     CDS             2152..4827
                     /gene="acnA"
                     /locus_tag="JN701_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="JN701_002607"
     CDS             complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="JN701_002607"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY7365016.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5651..6415
                     /gene="pgpB"
                     /locus_tag="JN701_002608"
     CDS             5651..6415
                     /gene="pgpB"
                     /locus_tag="JN701_002608"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY7365017.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6564..6872
                     /gene="lapA"
                     /locus_tag="JN701_002609"
     CDS             6564..6872
                     /gene="lapA"
                     /locus_tag="JN701_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY7365018.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6879..8048
                     /gene="lapB"
                     /locus_tag="JN701_002610"
     CDS             6879..8048
                     /gene="lapB"
                     /locus_tag="JN701_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY7365019.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8241..8978
                     /gene="pyrF"
                     /locus_tag="JN701_002611"
     CDS             8241..8978
                     /gene="pyrF"
                     /locus_tag="JN701_002611"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY7365020.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8978..9304
                     /gene="yciH"
                     /locus_tag="JN701_002612"
     CDS             8978..9304
                     /gene="yciH"
                     /locus_tag="JN701_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY7365021.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="JN701_002613"
     CDS             complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="JN701_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY7365022.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9917..10666)
                     /locus_tag="JN701_002614"
     CDS             complement(9917..10666)
                     /locus_tag="JN701_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7365023.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10756..10929)
                     /locus_tag="JN701_002615"
     CDS             complement(10756..10929)
                     /locus_tag="JN701_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365024.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="JN701_002616"
     CDS             complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="JN701_002616"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7365025.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3333 a         3084 c         3293 g         3386 t
ORIGIN      
        1 tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat acactaaggc
       61 tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt attagtcacc
      121 cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt ttaacatgaa
      181 attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg tctcatcaac
      241 agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa tgctggaaga
      301 cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg taacgccagc
      361 agggcaagaa ataattcgta tcgctcgcga agtcctgtcg aaagtcgatg ccataaaatc
      421 ggttgccgga gagcacacct ggccggataa aggctcactg tatatcgcca ccacgcatac
      481 ccaggcacgc tacgcattac caaacgtcat caaaggcttt attgagcgtt atcctcgcgt
      541 ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgccgtct ctaaaggcaa
      601 tgccgatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag tgatgttacc
      661 gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg caggcaaaaa
      721 agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct tcggctttac
      781 cggacgttca gaactggata ctgcctttaa ccgcgcaggg ttaacgccgc gtatcgtttt
      841 cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg gggtaggggt
      901 cattgccagc atggcggtgg atccggtcgc cgatccggac cttgtgcgcg ttgatgctca
      961 cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt ttttgcgcag
     1021 ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg tcgttgatgc
     1081 ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata taaaactgcc
     1141 ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt gccggaatat
     1201 attctctttt gttacttatt tttataccgt gcacaatatt tttatccctc atatttatag
     1261 ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaattccag ttttcaaata
     1321 tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat tcgctggatt
     1381 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
     1441 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
     1501 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
     1561 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
     1621 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
     1681 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
     1741 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
     1801 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
     1861 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
     1921 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
     1981 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
     2041 ctcttttatc aatttggttt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
     2101 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
     2161 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
     2221 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
     2281 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
     2341 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     2401 gcaagggtgc tgatgtagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     2461 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     2521 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     2581 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga
     2641 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     2701 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     2761 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttggcgtg
     2821 ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca gccggtttcc
     2881 atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga aggtattacc
     2941 gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt ggtggggaaa
     3001 ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg cgccaccatt
     3061 gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga tgctgtaacc
     3121 ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat
     3181 gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac cagtacgtta
     3241 gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgccc acaggatcgc
     3301 gttgcactgc ccgatgtacc aaaagcattt gccgccagta acgaactgga agtgaatgcc
     3361 acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta tcagttacct
     3421 gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa cccaagtgtg
     3481 ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc tgggcctcaa gcggcaaccg
     3541 tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt ctgattatct ggcaaaagcg
     3601 aaactgacac cgtatctcga cgaactgggg tttaaccttg tgggatacgg ttgtaccacc
     3661 tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat
     3721 ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg aaggccgtat ccatccgctg
     3781 gttaaaacta actggctggc ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat
     3841 atgaatatca acctggctgc tgagcctatt ggtcatgatc gtaaaggcga cccggtttat
     3901 ctgaaagata tctggccatc ggcacaggaa attgcccgtg cggtagaaca agtctccaca
     3961 gaaatgttcc gtaaagagta cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt
     4021 aacgtcacac gatccgatac ctacggttgg caggaggatt caacctatat tcgcttatcg
     4081 cctttctttg atgaaatgca ggcaacacca gcacctgtgg aagatattca cggtgcgcgg
     4141 atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc gggcagtatt
     4201 aagcccgaca gcccggcggg tcgatatcta caaggtcggg gtgttgagcg aaaagacttt
     4261 aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac cttcgccaat
     4321 attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg gcatttacct
     4381 gacagcgatg ttgtctctat ctatgatgcc gcgatgcgct ataagcagga gcaaacgccg
     4441 ctggcggtga ttgccgggaa agagtatgga tcaggctcca gccgtgactg gacggcaaaa
     4501 ggtccgcgtc tgcttggtat tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt
     4561 tcgaatttaa ttggcatggg catcttgccg ctggaatttc cgcaaggcgt gacgcgtaaa
     4621 acgttagggc taaccgggga agagaagatt gatattggcg acctgcaaaa tctacaaccc
     4681 ggcgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaagt cgtaccctgc
     4741 cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg cattttgcat
     4801 tatgtcattc gtaatatgct gaagtaacaa gtatttgctt gccggttatt ttgcttccgg
     4861 caagcaaatt aattccacaa tgcgagaggg ttatttgttc agcaaatgac ccattttctc
     4921 ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca atggtacgcg
     4981 ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt tattggttaa
     5041 caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag tgaagtcgcg
     5101 ctcatcagcg gcgaagccta actggtggtt agcctctacg gtatcgtaac cttgatcctg
     5161 cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct gacggtgata
     5221 cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc
     5281 acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat ggacgcgcgc
     5341 aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat cgtgtccggt
     5401 tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca gtttggcttc
     5461 tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta ttggcttatt
     5521 ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac ggaatggcac
     5581 aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga cttatcaaaa
     5641 aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac tattgcttgt
     5701 catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac aaagttggct
     5761 actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg tcattacaca
     5821 tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg ctgcctttgt
     5881 attatttgcc attctggcgg ccgcaatcct tgtgggacaa ggcgttaaat cctggatcaa
     5941 agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac atcatattcc
     6001 ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga aagaacagtt
     6061 ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag agacggggtt
     6121 tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg ccgttggttt
     6181 gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctgctctggg caacgggagt
     6241 catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag tagctacatt
     6301 gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt gtgggccatt
     6361 aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg
     6421 gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc catttcgcag
     6481 acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa tttattcgct
     6541 ctaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg gtgttagcga
     6601 tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg tttaattatc
     6661 tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt gctgcggggt
     6721 ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc
     6781 gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact gacgtggctg
     6841 tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg ttgtttctgc
     6901 ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg caacaaaaca
     6961 agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac ttcctgctta
     7021 gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag gatacaggca
     7081 ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg
     7141 ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct gacctatgaa cagcgtctgt
     7201 tggcgattca acaactaggg cgtgattaca tggccgccgg gttatacgac cgcgcggaag
     7261 acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgctctg caacagttgc
     7321 tacaaatcta ccaggctacc agtgagtggc agaaagcaat tgatgttgcc gaacgcctgg
     7381 tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt gagttagccc
     7441 tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg
     7501 cggcagataa aaacagagcc cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag
     7561 gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac agagaactgg
     7621 tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa actgccgaat
     7681 gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct gaattgatgc
     7741 ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat attacgcgcc
     7801 agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac cacttaaatg
     7861 aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa
     7921 aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca tacaccctct
     7981 actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc ggtcttgatg
     8041 gcctgtaatt tttaaaaaaa tccgacttta gttacaacat gctagttatt gatgttccat
     8101 tgtgctccgg caacgacgga gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat
     8161 tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc
     8221 atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga
     8281 attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg tcctttgtcg
     8341 acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg
     8401 ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt gacctgaaat
     8461 tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
     8521 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac
     8581 tggttccgtt tggcaaagat gcaccgcttt tgattgctgt aacagtgttg accagcatgg
     8641 aagctagcga cctggccgat cttggcgtga cactgtcacc tgcagattat gcagaacgtc
     8701 tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg
     8761 tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacaccgggc attcgtccgc
     8821 aggggagtga tgctggtgac cagcgccgca ttatgacgcc agaacaggcg ttggcggctg
     8881 gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcaaacac
     8941 tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct
     9001 ggtctactca acggaaaccg gacgtattga tgagccaaaa gcggccccag tacgccctaa
     9061 aggcgatggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg
     9121 cctgattacc ggtatcgatc tcgatgatgc cgaactgaca aaacttgcag cggaactgaa
     9181 gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa
     9241 gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac tcgcaggcgg
     9301 ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt gtgtattatt
     9361 cagttatttc gtactgaaca aaatcaagcg tattataatt gcgagtcgat aatcgacatt
     9421 tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg caccacctaa
     9481 tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc
     9541 gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccaa
     9601 agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc
     9661 tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga ataagcctgg
     9721 tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata tcacgcaggt gataatgact
     9781 tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca ggtaaatagt
     9841 cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc aaatcatccg
     9901 tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat
     9961 gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg
    10021 gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcactt
    10081 cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtga
    10141 atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa tgcacctgct
    10201 ggatgcactg gcgtgtcaac gggccaacca tactttcgct ttttttctgg tacacgccac
    10261 cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg
    10321 tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg gcattgctgc
    10381 tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga
    10441 gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca ctatcaagcg
    10501 aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct
    10561 ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac
    10621 cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct gtaattctta
    10681 taacgttata atactaaata aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac
    10741 ctgaaggcac gattgttaaa tctcccacgg tgccttcggg atgtctggcg tagattcagc
    10801 aacctggcgc aagagttctg ctttaagtat ctcgagatta tggatcgcag agtggtaatc
    10861 gcctgccaca aggatatcta acacaatatc aatacgttcg gcaacgcgct gggcatcaaa
    10921 ttcagacatc aaggacatcc ttatagcaaa agtggaaaat gaataatgca aaaattgccg
    10981 ctaaaagaga agtgtttaac agcaacggct aattatcatc caggaatacg atatataatg
    11041 acgggatata gcgctaagta tatatattca tctacttatg cgcgcttcag atagcgttta
    11101 taccagcgtt cgaaggcgac ggcgggcatc ggtttggcaa acaaaaatcc ttgccgctca
    11161 ttgatcccgt tcttggttaa aaaagcatct tcctttgcac tctctacacc ttcggcgatc
    11221 acctgaagat tcaatgcctg ggccacagcg acgatcgccc ggaccagtga ctgcgagaca
    11281 ggttgtttgt gaatatctcg aacaaaaacc tggtcaagtt tgatggcatc gatcggaaag
    11341 cgcgccagtt gcgaaagtga agagtagccg gtaccaaaat catccagatg cacttgcgca
    11401 cctagttggc taaattgttg aataacagac agtgccagtt catcattctc aatcagacaa
    11461 ctctctgtca gttcaacatc tatagggcag tattcaaaat tgagttcctg gagaacctgt
    11521 ttcagggcgg tgaaaatggt ttgatcggcg agctgacgtg cagaaatatt taccgccact
    11581 cgcaggttta tgcctttatc ccgccacttt gccacctggc gtacgacatc gagaatcacc
    11641 caacggccta aaggcacaat tagccctgac tcttcggcgt aggaaatgaa gtccaacggt
    11701 ggaatcaacc cacgttcagg tgactgccaa cgtactagtg cttccagact gcgcacttcg
    11761 ccacgccagg tgattttcgg ttgatagtga ataaccaact gatcgttttc cagtgcttta
    11821 cgcaagttgg tatccagcca gagatattca aatacccgtt gattcatttc tggggtaaaa
    11881 acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca ttgctgtgtc ggcgtgacga
    11941 ataatagccg tgctgtctga accatgttcg ggagagagtg caatacctac tgaacagctg
    12001 gtataaactt caattaaacc aatgcgaaag gggagccgta agcgggtcaa aattcgtgat
    12061 gccattgctt ccagcgcgct ttgtgaggtg ttggatgcca gtaccagaaa ctcatcccca
    12121 cctggacgcg ccaacacctg gtcatgttcg agacagctta aaatagccaa tgacacgtcg
    12181 cgtaataact ggtcaccaaa caaatgccca taggcgtcgt tgaccttttt gaaattatcc
    12241 aaatcaagat acacaacccc aactttattg ttatctgcat gattaatagc gtgatcgatt
    12301 aaatcctgca ttgcgttacg attcggcagt ccggtgatac tgtcggtatt tgccagaata
    12361 cgcagtcgct cctgagcgcg gcgctcttcg gtaatgtcgg tgccggaaca gattaaaaaa
    12421 atctcgcttt tgccactgcc gctgtggaca aatttattgc gaaacagaaa cagccgctgg
    12481 cctttacatg ttggtatcca cagttcgact tcatatgcat tgccgcttcg aaaaaataca
    12541 cggttattgc gcctggatgc cgcagcttca cgacggctca taaacagttt aaacacgctt
    12601 tggccaatga cgtcgtgttc tttcaaccca gtgtaatctt cacataaccg attgaagcgt
    12661 tggatattcc cccggctgtc gagaatcact attgcggaat gtgcttcaga aactacctgc
    12721 tcagcaaagg aaagcccgtg tgataaatca cgagcaactg ccggtgtgtc atgccatgca
    12781 gacgcgttgc cagcccattc ccgtttattg atttttcgtc ctaccagatg cacagaaaga
    12841 tcgctctcat cgacggtcag actcatcatc aggctggagg tgatgaccgt catttccctg
    12901 atgcgagcag cctgttcggc agataactgc aaagtacgat caggttctgt ggtttcggtg
    12961 gtagaaaagc gcaaaacatc gctgctttcc gtcaaccgcc agtatggatt gtgcgatccg
    13021 agaaagttgt acaacgttgt ggactcccta acggttttca tgatgcatat cctccggtta
    13081 aataaaagga acaaca
//
LOCUS       DADLTD010000116        13092 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLTD010000116 DADLTD010000000
VERSION     DADLTD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.116"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            4..1632
                     /locus_tag="JN701_002617"
     CDS             4..1632
                     /locus_tag="JN701_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY7365026.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1871..1930
                     /locus_tag="JN701_002618"
     CDS             1871..1930
                     /locus_tag="JN701_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7365027.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2247..2306
                     /gene="ibsE"
                     /locus_tag="JN701_002619"
     CDS             2247..2306
                     /gene="ibsE"
                     /locus_tag="JN701_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY7365028.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="JN701_002620"
     CDS             complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="JN701_002620"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY7365029.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="JN701_002621"
     CDS             complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="JN701_002621"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY7365030.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6770..8071)
                     /locus_tag="JN701_002622"
     CDS             complement(6770..8071)
                     /locus_tag="JN701_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY7365031.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8313..8933
                     /locus_tag="JN701_002623"
     CDS             8313..8933
                     /locus_tag="JN701_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY7365032.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            8997..10235
                     /gene="cca"
                     /locus_tag="JN701_002624"
     CDS             8997..10235
                     /gene="cca"
                     /locus_tag="JN701_002624"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY7365033.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="JN701_002625"
     CDS             complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="JN701_002625"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7365034.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11304..11672)
                     /gene="folB"
                     /locus_tag="JN701_002626"
     CDS             complement(11304..11672)
                     /gene="folB"
                     /locus_tag="JN701_002626"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY7365035.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11777..12394
                     /gene="plsY"
                     /locus_tag="JN701_002627"
     CDS             11777..12394
                     /gene="plsY"
                     /locus_tag="JN701_002627"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY7365036.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSTLIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12407..>13092)
                     /gene="ttdR"
                     /locus_tag="JN701_002628"
                     /gene_synonym="dan"
     CDS             complement(12407..>13092)
                     /gene="ttdR"
                     /locus_tag="JN701_002628"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAY7365037.1"
                     /translation="VDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQV
                     HFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQSL
                     QELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIML
                     RSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRLG
                     KPDEGYQVM"
BASE COUNT         3163 a         3682 c         3281 g         2966 t
ORIGIN      
        1 tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgcc
       61 aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa
      121 aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc
      181 aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa
      241 gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaacc gtctgtagaa
      301 ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt
      361 atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg
      421 catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa
      481 gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc
      541 tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgatt
      601 caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg
      661 gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg
      721 tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt
      781 attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa
      841 cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag
      901 gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga aatcgccaac
      961 cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt
     1021 gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgccag
     1081 accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa
     1141 accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag
     1201 gcggcggcta tcgttaagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa
     1261 gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa
     1321 ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg
     1381 aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg
     1441 ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc
     1501 gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt
     1561 tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa
     1621 aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg gattttttat
     1681 atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct
     1741 cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct catcaacaat
     1801 cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg ccttacagaa
     1861 ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg taagtttcgc
     1921 agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt
     1981 taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct agcccaaaaa
     2041 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc
     2101 gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
     2161 ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg
     2221 aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt
     2281 tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac
     2341 ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa
     2401 gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga
     2461 tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc
     2521 agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc accagtaaat
     2581 ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt
     2641 cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg
     2701 aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc agacggtcac
     2761 ccagtttgcg agcatttgcc agataggaga cgtgcccggc atgcaggatg tcaaagacac
     2821 cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacggcc agcttcagtt
     2881 cctcttcggt catcacgcca aagccggtat ctgcgcgccc acgcactgca ttttccagct
     2941 cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg
     3001 caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa
     3061 tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca
     3121 gcggcgcttt acccggttgc agcagcgaca taccttgttc ggaacgggtc actaacagag
     3181 ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct
     3241 tacatttacc gacaacagct tcaaattccg agagattcgg cgttaacagc gtagcgccgc
     3301 ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc gctttacgcg
     3361 ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca
     3421 gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc ggatcaacgc
     3481 cttcgaaacc ttcttcgaaa tccagacgga tcagctgttg gttgcgggaa agtacccgta
     3541 atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg
     3601 ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg accaggcgtg
     3661 cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc ggacgttctt
     3721 cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac
     3781 cgtaccagta acgatccagc attacatcac caaccaccat cactcctgca cgttcaaact
     3841 ctgacagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac
     3901 catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat cacgctctgc
     3961 ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat gatgaagttc
     4021 atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt ccataatgtc
     4081 gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta acttcggttt
     4141 ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg taattccgcc
     4201 ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag cgcgcatttt
     4261 ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca gcatcataat
     4321 ctcgcgacgc actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacacgcgc
     4381 acgcaccagc gcctgatgct cccacgtcca ggcctcgttt ttctgataat cagcaaatgc
     4441 ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg catccacttc
     4501 atacaaaatg ccggaagagg ttcgcgtatt gaacagatgc ataatgcgtt gcgccagacg
     4561 cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca
     4621 atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc cgcccagctt
     4681 gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat ttggcttacc
     4741 gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca tagcctctgc
     4801 cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga tatccgctgc
     4861 ggcgatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc
     4921 gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg catcggtcgc
     4981 cgtcggctgg taaagagtgt ttggatcgag caattcatcc agcaataatg gataacgcgc
     5041 cagctggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt taagcgccgc
     5101 agggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaggc
     5161 ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac ttagcagatg
     5221 tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct tatccagctc
     5281 tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc taagatgcgc
     5341 cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc
     5401 cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg
     5461 gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc
     5521 catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt gggtttgttc
     5581 gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg ccactcgcaa
     5641 ttgttcagca tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggcag
     5701 taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac
     5761 gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg
     5821 tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa
     5881 atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac
     5941 gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc
     6001 ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc cactttcgcc
     6061 aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccaa
     6121 cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg
     6181 tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat ctgaggagaa
     6241 attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc
     6301 ctgcgcattg cacggcgttc cccactcgcg gcagcaagcg tcatacagcc agtcacgcgc
     6361 ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct cttcagtaac
     6421 caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag
     6481 ctcgcgcatt aacccggctt cgtcactcac attgctgagc gcctcctgca accatgccgc
     6541 gtaatgctgc cattcatcag cctgcggcga ttggctttcc agttccgtca gccactctgg
     6601 atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact tcgcctgtgc
     6661 gctaagtgat ttctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg
     6721 ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc
     6781 cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa
     6841 tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg
     6901 cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca cgcgtcagac
     6961 gtggcaactg gtcacggtag cgatcgccca acggctggca gaaaacgctt ttcagttcag
     7021 cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc attttactct
     7081 gcgctttggc atctaaaaac ggctgccagg ctttgcttac cagccattcg gtcaacgcca
     7141 gcttcgccat tgccgttttg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcga
     7201 tggtcgcttc gcattgagtc agcaaatcgc gtaagtgagt gctcgctttg cgcggcacaa
     7261 taccaccgaa cagcatcagt gtatgacgga ccagaccaat ggctgccagc acctgttctt
     7321 tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca
     7381 gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca tgcaaaatgg
     7441 tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt
     7501 tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca
     7561 ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt
     7621 cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca tcgaccgcca
     7681 ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat
     7741 cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg
     7801 gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg
     7861 caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt
     7921 gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat
     7981 ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa
     8041 caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa
     8101 gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca
     8161 aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga
     8221 cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc
     8281 gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa
     8341 ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc tatgtttctg
     8401 atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg
     8461 ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg
     8521 tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa
     8581 gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca
     8641 ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga
     8701 gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac gagctgattg
     8761 tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag cgcaccatca
     8821 tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg
     8881 tactgccaca cctgatccca acgcgcaaac gcaaagagcg ctggatgaac taaatcgcct
     8941 tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga
     9001 agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca
     9061 gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg
     9121 taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat gcgctggcac
     9181 gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc gcaccggatg
     9241 tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg gcccaggacg
     9301 ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc
     9361 gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg gcgcgttttg
     9421 ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg ctgatgcgcg
     9481 agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg
     9541 aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg
     9601 cacttcgcgt tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt
     9661 ggcatccgga aatcgacacc ggtattcata ccttaatgac gctctcaatg gcggcgatgc
     9721 tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga
     9781 cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt aagttagtgg
     9841 aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg
     9901 ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat
     9961 tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca
    10021 gcgaggctga cgtacgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt
    10081 ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg
    10141 gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca
    10201 gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg ttaacacgaa
    10261 ccatcccctc gccccttcgg ggagagggtg aggggaacag accagcaccg gtgcgaacaa
    10321 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    10381 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    10441 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    10501 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc ggttgtcagg
    10561 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    10621 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    10681 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    10741 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    10801 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    10861 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    10921 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    10981 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    11041 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    11101 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    11161 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    11221 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    11281 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    11341 attacaccaa cattcgccgc ccgcgccact gcgcctggct tgctgagttt gatgcgcacc
    11401 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    11461 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    11521 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    11581 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    11641 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    11701 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    11761 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    11821 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    11881 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    11941 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    12001 attaggtgtc agccccttct ggctaggctt aattgccatc gccgcctgtc ttggacacat
    12061 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    12121 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    12181 attgagcgga tactcgtcgc tgggagcgat tgtcagtaca ctgattgctc cgttttatgt
    12241 ctggtggttt aagccacaat tcaccttccc agtttcgatg ctctcttgcc tgatcctgct
    12301 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    12361 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    12421 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    12481 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    12541 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    12601 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    12661 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    12721 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    12781 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    12841 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    12901 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    12961 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    13021 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    13081 cgtcatcgac ca
//
LOCUS       DADLTD010000117        12990 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLTD010000117 DADLTD010000000
VERSION     DADLTD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.117"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="JN701_002629"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="JN701_002629"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY7365038.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="JN701_002630"
     tRNA            647..722
                     /locus_tag="JN701_002630"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="JN701_002631"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="JN701_002631"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAY7365039.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /locus_tag="JN701_002632"
     CDS             1828..2451
                     /locus_tag="JN701_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY7365040.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGQGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2605..4125)
                     /locus_tag="JN701_002633"
     CDS             complement(2605..4125)
                     /locus_tag="JN701_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7365041.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4431..5921
                     /gene="ygjG"
                     /locus_tag="JN701_002634"
     CDS             4431..5921
                     /gene="ygjG"
                     /locus_tag="JN701_002634"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY7365042.1"
                     /translation="MITTFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     LAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(5963..6295)
                     /locus_tag="JN701_002635"
     CDS             complement(5963..6295)
                     /locus_tag="JN701_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAY7365043.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6514..7497
                     /gene="ebgR"
                     /locus_tag="JN701_002636"
     CDS             6514..7497
                     /gene="ebgR"
                     /locus_tag="JN701_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY7365044.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEYYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7681..10773
                     /gene="ebgA"
                     /locus_tag="JN701_002637"
     CDS             7681..10773
                     /gene="ebgA"
                     /locus_tag="JN701_002637"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY7365045.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSITTYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10770..11219
                     /locus_tag="JN701_002638"
     CDS             10770..11219
                     /locus_tag="JN701_002638"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY7365046.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11282..12715
                     /locus_tag="JN701_002639"
     CDS             11282..12715
                     /locus_tag="JN701_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7365047.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         3134 a         3486 c         3246 g         3124 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat ctcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacca gccaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgattag tagaactgat atgtctatag tctgataaga cgaaccgcct tttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agaaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacaaggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gcgcggcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca gtgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgaccg cgccgctttt gatattacgc aactggatta atcgccgcat
     2461 ccgccagtgg cgcggtgcaa ttgccggatg cgacgcttga cgcgccttat ccggcctaca
     2521 cccactacac accccgcagg cctgataaga tgcgccagca tcgcatcagg cattgtgctc
     2581 caaccgccgg atccggcata ccgattaatg cagcaccgtc accgcgtctt ccagtcggct
     2641 ggcgcggtgt ttcaccatcg ccgacacctg cgcactctct tccaccagct cagcattttt
     2701 ctgggtgatc aggttaagct catccactgc acgggtcagg ctggaaagcc catcggcctg
     2761 ttccagcgtt gaatggctaa tctgggcgat caactgggtg acgtttttca cctgtgccac
     2821 aatatcttcc atcgtccgtc cggcggcgtg tacctgctgc gaaccggatt gcaccttatc
     2881 agcactggca tcaatcagct tgcgaatatc gttggcagca ttagcgctgc ggctggctaa
     2941 atgacgcact tcccctgcca ccactgcaaa acctttgccc tgttcgccgg cacgcgccgc
     3001 ttccaccgcc gcattcaggg ccagaatatt ggtctgaaac gcaatatcgt taatcagcga
     3061 agtaatggtg ccaatgcgct gggtactgtc ggcgatatcg tccattgtct tgatcaccgt
     3121 ggtcatcgcc tccccaccct gcactgccgc attactggcg atgatagaaa gtttatcggc
     3181 agccgacgcc gtggcagagt tctgtttcac cgacgccgcc atttggttca tggtcgccac
     3241 cgtttgctga acgttatcaa ctgtctgctg ggtatgttcg ttcagttcat cggtgccttt
     3301 cgccagcgtc tcactgccat ttctgacact ggacacctgg cttgagacat cgttaattag
     3361 ccaacggcac atcaggccaa gctgccctac cgcgcgtaat gtcagcccca gctcatcgct
     3421 gcgattcagg tgctcaacac tattacgctc tccggtcgcc accttcagtg cctgacgggc
     3481 gacattttct atcgggcgca caatctgcca ttcaaaacaa gcgcttgcca acaataccac
     3541 taacacacag aggaaatacg tcaccaccgg ggcagcaaca aaccaaagca tggccgccag
     3601 caagataaac atcagggtca tcactccacg cgcccgccag cgaagcggta atgaaggcag
     3661 tttacccagc cagcctttac gcaccaccag gcctttatga atacgcttac tggtacgtcc
     3721 ggcgttcagc gctttgtaca gcggctccac cgccgcaatc tcttcatccg tcgcccgggt
     3781 acgaatcgac atatagccac tgatttttcc ctcgcgcacc atcggtaccg cattggcccg
     3841 cacccaataa tggtcgccat ttttgcggcg atttttcacg atgccgctcc agggctcccc
     3901 ttttttcagg gtgaaccaca tatccgcaaa cgccgctttt ggcatatccg ggtgacgcac
     3961 catgttgtgc ggctgccctt gtaactcttg caaggtaaag ccgctcacct gcacaaaagt
     4021 gtcattagca tgagtgatat agctttgcag atcggtagtg gacatcagag tggtatcgtc
     4081 cgccagcggg gtattttgct gggtgacata cggatgagaa gacatgttcg cgtcctatgc
     4141 aggttatatg gttgttaact tcttgtcaga gtttatgtcg gccccgctgc ggttatcttt
     4201 aaccgattaa tttgatttag atcgcaattt gcgattaaag cacaaatcta attccttgat
     4261 ttaaaatact ttcactctgt tactatacga aaacgttaat tatcttgccc aaaatcaggc
     4321 aattatcgcc ctgaaaacgt gcatttgcac agcaatcatc aaatccatac ccgacaaaaa
     4381 ccgtgcaaaa taacaacgaa tgttaacaaa tagcattaaa tactgcgcaa atgataacca
     4441 catttgtgtt tatcccgatt ttcgcgatcg cagccggagt ggcgcaatcc ctgcaatact
     4501 taaatcggta tcatgtgata cgcgagcctc cggagcatat tttgaacagg ttaccttcga
     4561 gcgcatcggc tttagcgtgc agcgcccacg ccctgaatct cattgagaag cgaacgctgg
     4621 atcatgagga gatgaaagca cttaaccgag aggtgattga atacttcaaa gagcatgtca
     4681 atccggggtt tttagagtat cgcaaatctg ttaccgccgg cggggattac ggagccgtag
     4741 agtggcaagc gggaagttta aatacgcttg tcgacaccca gggacaggag tttatcgact
     4801 gcctgggagg ttttggaatt ttcaacgtgg ggcaccgtaa tccagttgtg gtttccgccg
     4861 tacagaatca acttgcgaaa caaccgctgc acagccagga gctgctcgat ccgttacggg
     4921 cgatgttggc gaaaaccctt gctgcgctaa cgcccggtaa actgaaatac agcttcttct
     4981 gtaatagcgg caccgagtcc gttgaagcag cgctgaagct ggcgaaagct taccagtcac
     5041 cgcgcggcaa gtttactttt attgccacca gcggcgcgtt ccacggtaaa tcacttggcg
     5101 cgctgtcggc cacggcgaaa tcgaccttcc gcaaaccgtt tatgccgtta ctgccgggct
     5161 tccgtcatgt gccgtttggc aatatcgaag ccatgcgcac ggctcttaac gagtgcaaaa
     5221 aaaccggtga tgatgtggct gcggtgatcc tcgaaccgat tcagggtgaa ggtggcgtaa
     5281 ttctgccgcc gccgggctat ctcaccgccg tacgtaagct atgcgatgag ttcggcgcac
     5341 tgatgatcct cgatgaagta caaacgggca tggggcgcac gggcaagatg ttcgcctgcg
     5401 agcatgagaa cgtacagccg gatatcctct gccttgccaa agcgctcggc ggcggcgtga
     5461 tgccgattgg cgcgaccatc gccactgaag aggtgttttc agttctgttc gacaacccat
     5521 tcctgcatac caccaccttt ggcggcaacc cgctggcctg tgcggcggcg ctggcgacca
     5581 tcaatgtgtt gctggagcag aacttactgg ctcaggctga gcaaaaaggc gatatgttgc
     5641 tggacggttt ccgtcaactg gcgcgggaat atcccgatct ggtacaggaa gcgcgtggta
     5701 aagggatgtt gatggcaatt gagtttgttg ataacgaaat cggctataac tttgccagcg
     5761 agatgttccg ccagcgcgta ctggtggccg gaacgctcaa taacgccaaa acgatccgca
     5821 ttgaaccgcc actgacactg accattgaac agtgtgaact ggtgatcaaa gcggcgcgta
     5881 aggcgctggc ggccatgcga gtaagtgtcg aagaagcgta atatcaaatc ggatggcgat
     5941 acgacgtcgc catccgatct ttttatacga cgcgtacgcc cgcaggcatc atccgctccg
     6001 gcgttaacaa cacgctttca ctgccgtcat cggtttccgc acacaacagc atgcattccg
     6061 acgtttcacc acgcattttc gctttttgca gattgcataa gaccaccacc gttttcccca
     6121 tcagctcttc ttcgctgtag tacggcacca ggctggtcac ggtttgcagt gttttttccc
     6181 ccacatcaac ctgtacgatg tacagcttgt cggcgttttc atggcgtttc acttccacaa
     6241 tctttccgac gcgcatttcc agacgtgcaa aatcagcgta agccacggtt tccattgttt
     6301 ccttccttcg ggtaaaattt tactaaacta tagcaaaagt ttttctcaat cctgtaggct
     6361 aaaaatggag aatgcaggcg tgatcacatt cgtgagccgc tgtgttaccg ttacagcgtc
     6421 aaagaaacgc gctttattta ctgaaaacag gtgacccgat aagtacttcc tctacaatgg
     6481 gggcgcacat cagggaaagt aaaaaaggta aacatggcaa cactaaaaga catcgcaatc
     6541 gaagctggcg tatccctggc gacagtatcc agggtcttaa atgacgatcc gacattgaat
     6601 gtgaaagaag agacgaaaca tcgcattctc gagatcgccg aaaagctgga gtacaagacc
     6661 agtagtgccc gcaaactcca gacaggtgca gtcaaccaac accatattct ggctatctac
     6721 agctaccaac aggagctgga gatcaacgat ccttactatc tggcgatccg ccacggcatt
     6781 gaaactcagt gcgaaaagct gggcattgag ctcaccaact gttatgaaca cagcggatta
     6841 ccagacatta aaaacgtcac cggtatttta attgtcggca aacccacgcc cgcccttcgc
     6901 gccgccgcct gtgcgttgac cgacaatatc tgttttatcg actttcacga acccggcagc
     6961 ggttacgatg cggtggatat cgatctggca cgcatcagta aagaaatcat cgacttctat
     7021 atcaatcagg gcgttaatcg tattggtttt attggcggtg aagatgagcc aggcaaagcg
     7081 gatattcgtg aggtcgcctt tgcggaatat ggccgactga aacaagtggt acgcgaagag
     7141 gatatctggc gcggaggctt ttccagttcg tcgggttatg aactggcaaa acagatgctg
     7201 gcgcaggaat actatccgaa ggcactgttt gttgcttccg attccattgc tatcggcgta
     7261 ctgcgggcaa ttcatgaacg aggcctgaac atcccacagg atatttcgct tatcagcgtt
     7321 aacgatatcc ccaccgcgcg atttaccttt ccgccgctct ccaccgtgcg catccattcc
     7381 gagatgatgg gaagtcaggg cgttaacctg gtgtatgaaa aagcccgcga tggtcgcgcg
     7441 ctgccgctgt tagtcttcgt tcccagcaaa ttaaaactgc gcggcacgac ccgttaaatc
     7501 cccttacaca ctgtccggca atcgtttttg ccggacagtg ctgccgttta ttttcgtgat
     7561 ccagttaaag taaatgcatt tatctgctac tttttagtaa aaattttact aaactcccca
     7621 gcaattacac aaactaccat caccatgaat ggttccgatt tctctctacc gggaggccct
     7681 atgaatcgct gggaaaacat tcagctcacc cacgaaaacc gacttgcgcc gcgtgcgtac
     7741 tttttttcat atgattctgt tgcgcaagcg cgtacctttg cccgcgaaac cagcagcctg
     7801 tttctgccct taagcggtca gtggaatttc cacttttttg accatccgct gcaagtacca
     7861 gaagccttca cctctgagtt aatggctgac tgggggcata ttaccgtccc cgccatgtgg
     7921 caaatggaag gtcacggcaa actgcaatat accgacgaag gttttccgtt ccccatcgat
     7981 gtgccgtttg tccccagcga taacccaacc ggtgcctatc aacgtatttt caccctcagc
     8041 gacggctggc agggtaaaca gacgctgatt aaatttgacg gcgtcgaaac ctattttgaa
     8101 gtctatgtta acggtcagta tgtgggtttc agcaagggca gtcgcctgac cgcagagttt
     8161 gacatcagcg cgatggttaa aaccggcgac aacctgttgt gtgtgcgcgt gatgcagtgg
     8221 gcggactcta cctacgtgga agaccaggat atgtggtggt cagcggggat cttccgcgat
     8281 gtttatctgg tcggaaaaca cctaacgcat attaacgatt tcactgtgcg taccgacttt
     8341 gacgaagcct attgcgatgc cacgctttcc tgcgaagtgg tgctggaaaa tctcgccgcc
     8401 tcccctgtcg tcacgacgct ggaatatacc ctgtttgatg gcgaacgcgt ggtgcacagc
     8461 agcgccattg atcatttggc aattgaaaaa ctgaccagcg ccagctttgc ttttactgtc
     8521 gaacagccgc agcaatggtc agcagaatcc ccttatcttt accatctggt catgacgctg
     8581 aaagacgcca acggcaacgt tctggaagtg gtgccacaac gcgttggctt ccgtgatatc
     8641 aaagtgcgcg acggtctgtt ctggatcaat aaccgttatg tgatgctgca cggcgtcaac
     8701 cgtcacgaca acgatcatcg caaaggccgc gccgttggaa tggatcgcgt cgagaaagat
     8761 ctccagttga tgaagcagca caatatcaac tccgtgcgta ccgctcacta cccgaacgat
     8821 ccgcgttttt acgaactgtg tgatatctac ggcctgtttg tgatggcgga aaccgacgtc
     8881 gaatcgcacg gctttgctaa tgtcggcgat atcagccgta ttaccgacga tccgcagtgg
     8941 gaaaaagtct acgtcgagcg cattgttcgc catatccacg cgcagaaaaa ccatccgtcg
     9001 atcatcatct ggtcgctggg caatgaatcc ggctatggct gtaacatccg cgcgatgtac
     9061 catgcggcga aagcgctgga tgacacgcga ctggtgcatt acgaagaaga tcgcgatgct
     9121 gaagtggtcg atattatttc caccatgtac acccgcgtgc cgctgatgaa tgagtttggt
     9181 gaatacccgc atccgaagcc gcgcatcatc tgtgaatatg ctcatgcgat ggggaacgga
     9241 ccgggcgggc tgacggagta ccagaacgtc ttctataagc acgattgcat tcagggtcat
     9301 tatgtctggg agtggtgcga ccacgggatc caggcacagg acgaccacgg caatgtctgg
     9361 tataaattcg gcggcgacta cggcgactat cccaacaact ataacttctg tcttgatggt
     9421 ttgatctatt ccgatcagac gccgggaccg ggcctgaaag agtacaaaca ggttatcgcg
     9481 ccggtaaaaa tccacgcgcg ggatctgact cgcggcgagt tgaaagtcga aaataaactg
     9541 tggtttacca cgcttgatga ctacaccctg cacgcagagg tgcgcgccga aggtgaaacg
     9601 ctcgcgacgc agcagattaa actgcgcgac gttgcgccga acagcgaagc ccccttgcag
     9661 atcacgctgc cgcagctgga cgcccgcgaa gcgttcctca acattacggt gaccaaagat
     9721 tcccgcaccc gctacagcga agccgggcat tctatcacca cttatcagtt cccgctgaag
     9781 gaaaacaccg cgcagccagt gcctttcgca ccaaataatg cgcgtccgct gacgctggaa
     9841 gacgatcgtt tgagctgcac cgttcgcggc tacaacttcg cgatcacctt ctcaaaaatg
     9901 agtggcaaac cgacatcctg gcaggtaaat ggcgagtcgc tgctgacccg cgagccaaag
     9961 atcaacttct tcaagccgat gatcgacaac cacaagcagg agtacgaagg gttgtggcag
    10021 ccgaatcatt tgcagatcat gcaggaacat ctgcgcgact ttgccgtaga acagagcgat
    10081 ggtgaagtgt tgatcatcag ccgcacagtt attgcaccgc cggtgtttga cttcgggatg
    10141 cgctgtacct acatctggcg catcgctgcc gatggccagg ttaacgtggc gctttccggc
    10201 gagcgttacg gcgactatcc gcacatcatt ccgtgcatcg gtttcaccat ggggattaac
    10261 ggcgaatacg atcaggtggc gtattacggt cgtggaccgg gcgaaaacta cgccgacagc
    10321 cagcaggcta acatcatcga tatctggcgc agcaccgtcg atgccatgtt cgagaactat
    10381 cccttcccgc agaacaacgg caaccgtcag catgtccgct ggacggcact gactaaccgc
    10441 cacggcaacg gtctgctggt ggttccgcag cgcccaatta acttcagcgc ctggcactat
    10501 acccaggaaa acatccacgc tgcccagcac tgtaacgagc tgcagcgcag tgatgacatc
    10561 accctgaacc tcgaccacca gctacttggc ctcggctcca actcctgggg cagcgaggtg
    10621 ctggactcct ggcgcgtctg gttccgtgac ttcagctacg gctttacgtt gctgccggtt
    10681 tctggcggag aagctaccgc gcaaagtctg gcgtcgtatg agttcggcgc agggttcttt
    10741 tccacgaatt tgcacagcga gaataagcaa tgaggatcat cgataactta gaacagttcc
    10801 gccagattta cgcctctagc aagaagtggc aacgctgcgt tgaagcgatt gaaaatatcg
    10861 acaacattca gcctggcgtc gcccactcca tcggtgactc attgacttac cgcgtggaga
    10921 cagactccgc gaccgatgcg ctatttaccg ggcatcgacg ctattttgaa gtgcattact
    10981 acctgcaagg gcagcaaaaa attgaatatg cgccgaaaga gacattacag gtagtggaat
    11041 attatcgtga tgaaactgac cgtgaatatt taaaaggctg cggagaaacc gttgaggtcc
    11101 acgaagggca aatcgttatt tgcgatatcc atgaagcgta tcggtttatc tgcaataacg
    11161 cggtcaaaaa agtggttctc aaagtcacca tcgaagatgg ttatttccat aacaaataac
    11221 aactacggcg gcaaaaggag tttgccgcca ccgctaccct acacattttc ggagatgtgt
    11281 tatgtctgat accaaacgta atacaatcgg caaattcggc ttgctctcgc tgacttttgc
    11341 tgccgtttac agctttaaca acgttatcaa taataatatt gagcttggac tggcctcggc
    11401 accgatgttt ttcctcgcga cgatttttta ttttattccc ttctgtctga tcatcgcaga
    11461 atttgtttcg ttaaataaaa actcagaagc cggtgtctac gcgtgggtaa aaagttcgct
    11521 gggcggacgt tgggcattta ttactgccta tacctactgg ttcgtaaacc tgttcttttt
    11581 cacctcactg ttgccgcgcg ttattgctta tgcttcgtat gccttcctcg gatacgaata
    11641 tattatgacg ccggttgcca ccaccattat cagtatggtg ctgttcgcct tctccacctg
    11701 ggtttccacc aacggggcga aaatgttggg gccaattacc tccgtcactt caacgctgat
    11761 gctgctgtta acgctctcct acattttact ggcaggtacg gcgctggttg gcggcgtaca
    11821 gcctgctgac cccatcaccg ttgacgcgat gatcccgaac ttcaactggg cgttcctcgg
    11881 cgttaccacc tggatcttta tggccgcagg tggcgcggag tccgtcgctg tgtacgttaa
    11941 cgacgtcaaa ggcggttcga aatcgttcgt taaagtgatc atcctcgccg ggatttttat
    12001 cggcgtactg tattccgtct cctcggtgct gattaacgtc ttcgtcagca gcaaagagtt
    12061 gaaatttacc ggcggatcgg tgcaggtatt ccatggcatg gcggcgtatt ttggtctacc
    12121 ggaagcgttg atgaatcgct ttgtcggtct ggtgtccttt accgcgatgt tcggttccct
    12181 gctgatgtgg accgcaacgc cggtgaaaat tttcttctcc gaaatcccgg aaggcatctt
    12241 tggtaagaaa accgtcgaac tgaacgaaaa cggcgttccg gcgcgcgcag cgtggatcca
    12301 gttcctgatc gtcatcccgc tgatgattat cccgatgctc ggttccaata ccgtgcagga
    12361 tctgatgaat actattatta atatgaccgc cgcagcgtcc atgcttccgc cgttattcat
    12421 catgctggct tacctgaatt tacgcgccaa attagatcac ctgccacgcg atttccgtat
    12481 gggctcccgc cgcaccggta ttatcgttgt ttcaatgctg attgcgatat ttgccgtagg
    12541 gtttgtcgct tcgacattcc cgactggcgc gaatattctg accatcattt tttataacgt
    12601 cggcggtatt gttatcttcc ttggctttgc gtggtggaaa tacagtaaat atataaaggg
    12661 attaacggct gaagagcgcc atattgaagc gacgccagcc agcaatgttg attaagcaga
    12721 ataataaaaa atgagcaggc attttccctc ttcctggatg aggagaggga aaatagtttc
    12781 tgcctttata tttttataat tacaacgata aaaggctgta ctttttcttt agctcatgga
    12841 ttaacacaat gaaattaatc actgcaccat gcagagcatt acttgctctg ccgttttgct
    12901 acgccttttc tgcggcagga gaagaagcac gtccggcaga acatgacgac acaaaaacac
    12961 ccgcaattac ctcgacatct tctccttcat
//
LOCUS       DADLTD010000118        12952 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLTD010000118 DADLTD010000000
VERSION     DADLTD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.118"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="JN701_002640"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="JN701_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY7365048.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="JN701_002641"
     CDS             1946..2230
                     /locus_tag="JN701_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY7365049.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="JN701_002642"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="JN701_002642"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY7365050.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="JN701_002643"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="JN701_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY7365051.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="JN701_002644"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="JN701_002644"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY7365052.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7227..7487
                     /locus_tag="JN701_002645"
     CDS             7227..7487
                     /locus_tag="JN701_002645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7365053.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7494..7790
                     /locus_tag="JN701_002646"
     CDS             7494..7790
                     /locus_tag="JN701_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7365054.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7872..10514
                     /gene="yfcU"
                     /locus_tag="JN701_002647"
     CDS             7872..10514
                     /gene="yfcU"
                     /locus_tag="JN701_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HAY7365055.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     NTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVDT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10534..11286
                     /locus_tag="JN701_002648"
     CDS             10534..11286
                     /locus_tag="JN701_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7365056.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            11302..11811
                     /locus_tag="JN701_002649"
     CDS             11302..11811
                     /locus_tag="JN701_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7365057.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11808..12296
                     /locus_tag="JN701_002650"
     CDS             11808..12296
                     /locus_tag="JN701_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7365058.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            12293..12832
                     /locus_tag="JN701_002651"
                     /pseudo
     CDS             12293..12832
                     /locus_tag="JN701_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         3261 a         3088 c         3439 g         3164 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgttgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgttgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgttgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatggttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgat ttaatgcccg taccgcctgc agtagcagaa
     2941 tattctaccg gcttgcggat gggtgacacc gcagagcaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgttc
     3361 ggtccggcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacaat gagcgatctg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgcttg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgtgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgacgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat cggcaactgc aaaacggtgc
     3961 aagaagcgga agctctggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg tggtgggctg gagttggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagcacaac attagagatg atcctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagtc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggttatgac gccgaagctc gggcgtttgg cgaactggcg atgacgccac
     4561 aatcgcaggc gctgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattgaaaca acagatggtg gcggaagttg agcaaaattg cgccgctcat accatctttg
     4981 cttcgaatac gtcatcttta ccgattggtg atatcgccgc tcacgccacg cgacctgagc
     5041 aagttatcgg cctgcatttc ttcagtccgg tggaaaaaat gccgctggtg gagattattc
     5101 ctcatgcggg tacatcagcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccaaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gtccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggtgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgacctgca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtattggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaatgggcg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggtac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgtgttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaacctca ctactatcga agactctcct tatctataaa
     6661 taaaacactt tctacattca ttcggaaatt attcttctgc acgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagtt gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt
     6841 gtttatatat tatattgtca aaagtggata aatgccaatc gcattatatg ttcattgttt
     6901 gttttttaag ctgttgatat tcattattga tatactttct attttttcta aatacttatt
     6961 ccgtattttt taaggatgtc cttaagataa tctttcttct gtcaatatat taattaaatc
     7021 tacatttact ctttagtaaa attaaattgc atcaggggtg tggaaacatc actatgcggc
     7081 cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat gccgtattca
     7141 atggctgggt ataaacattg gctattgact gaatcaatgt attgcaatgt ttatattttt
     7201 aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg
     7261 caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg
     7321 cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac gatcacaaac
     7381 tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc accaccacgc
     7441 cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tacttaggaa acaatgacca
     7501 ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc attttcaata
     7561 ccgataccgg tgcggcattt aacaatgtta gcctggcgat cggtgactct ctgggtacct
     7621 cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct actaataaag
     7681 gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct gatgcgccag
     7741 atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa ggcatacccc
     7801 catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa taaaaacgga
     7861 tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg tggtgcattg
     7921 cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt cagtttgatt
     7981 cccgttttct ggaattaaaa ggcaacacaa aaattgatct gaagcgtttt tccagccagg
     8041 gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag ccattggcgg
     8101 aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc tatgcttgtc
     8161 tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa aatctgcaat
     8221 ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa attaaggctg
     8281 atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa tatacttatc
     8341 ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc gcggactaca
     8401 gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac gagatcagcg
     8461 gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac tggcagacta
     8521 actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa actcaaaaaa
     8581 aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa gccaaactgg
     8641 cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat gttggtggta
     8701 gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg ccagacattt
     8761 ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt gtgatttacg
     8821 aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct gtctccggta
     8881 cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac atcagcaccg
     8941 cctcgatgcc atacctcact cgtccaggtc agattcgcta taagatcatg atgggccgtc
     9001 cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa gcttcctggg
     9061 ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac tatcagtctg
     9121 cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt gatgttactc
     9181 actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg gacggtaact
     9241 ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt acttttgctg
     9301 gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat gccagtgaca
     9361 gcggaatggt acgcacgggc aacgacaaag agatgtacac cgccacttat aaccagaact
     9421 tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac tgggatcgcg
     9481 aggagcagat aaactacaac atcatgctct cgcactattt caatatgggc agtattcgta
     9541 atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc gacaaaggca
     9601 tgtacatttc gctcagtatg ccgtggggcg acaacagtac cgttagctat aacggtaact
     9661 atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat gacgcgactc
     9721 actatcagtt gaacgtcgac accagtgaca aacacaccag cgttgacggc tattacagcc
     9781 atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg caatacacct
     9841 ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc gcacttcacc
     9901 gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt gccgatgttc
     9961 cggtggaagg taacggggct gctgtttata ccaatatgtt tggtaaagcc gtcgtttctg
    10021 acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacagattg cctgaaaacg
    10081 ctgaagcaac ccagtcggtg gtgcaagcca cgctaactga aggtgccatt ggctaccgta
    10141 aatttgctgt cattagcgga caaaaagcga tggcggtgct gcgcctgagc gacggcagcc
    10201 atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc cttgtcgatg
    10261 atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt gtgttctgga
    10321 gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat ctgtttaacg
    10381 gcttgttact gccatgccag cataaaggca atgtagcacc tatcacttcg ccggcggtca
    10441 aaccggcgat tcaggaacag acacagcggg tgacgccaac ggaacctccg acttcaattt
    10501 cagtaaacca gtaacgtgat taaggaatga tccatgttta atctgactaa cactgcaaaa
    10561 atcgttgtcc cggcactggc gctgctggcg acagcggtca gtttctccag ccacgcttcg
    10621 gtgacgcccg acaggacacg gctggtgttt aacgaaagcg acaagtccat cagcgtcacg
    10681 ctgcgcaaca acgatcccac actgccttat ctggcgcaga gctggataga ggatgcaaag
    10741 ggcaacaaaa tcagctctcc gcttgcggtg cttccgccag tgcagcgcat cgatgcgatg
    10801 atgaacggcc aggtgaaggt gcaggggctg ccggatatca acaaactgcc ggccgaccgc
    10861 gagagcgtgt tctatttcaa cgtgcgcgaa attccgccga aatcaaataa accgaacaca
    10921 ctgcagattg cgctgcagac gcgtatcaaa ctgttctggc gaccgaaggc gctggagaac
    10981 accagcatga aaagtccgtg gcagcataag gtgacgctca tccgtaacgg ccaggacttc
    11041 accgtcaata acccgacgcc gtactacgtg atcatcagca acgccagcgc taaaaaaggc
    11101 ggcaacccgc cggcgggatt cacaccgctg atgatggagc cgaaaacctc cgcaccgctg
    11161 aatgcgaaga tggacagcgt gccggtgctg acttacatca acgactacgg cgcgcggatg
    11221 ccgctgtact tcaaatgcac cggcaacagc tgccaggtgg atgaagagca gagccgcaaa
    11281 ggctgatgcc gggagggact tatgagtcac aagtttgtag cgggcgcagg gttgctggtg
    11341 ctggccggat ggggcatcag cggtttggct gttgctggca gtgatatcaa acaggtggat
    11401 ctgacgctgc gaatcctagt cgatggaccg ccgccttgct ccgtgaaggg aagcgctgtg
    11461 aaatttggca atgtgataat caacaggatc gatggtacca attaccaaca ggatgcgaaa
    11521 tacaccctca actgcggtaa taaagtcagc gatgacttac ggatgcagct caaaggcaat
    11581 accgccgtga tcaacggaga aacggttctc agcacggata tcgccggcct gggggtgcgg
    11641 atagaaaacg ccgccgacaa ctccctgttt gcagttggtg aaaataactg gacgcctttt
    11701 aatgtcaaca ctcagcctca gctgaaagcg gtgccggtaa aacagagcgg ggtgcaactg
    11761 actccgtcgg agtttaacgc cacgatgacc atggtggtgg attaccaatg aaaaaattcc
    11821 tgctcctttt actgtgcgct ggttgcgggg ctgtgctggc ggcggaagat gacatcacat
    11881 tccacggtac gctgatgtcg ccgcccgcct gtagcatcag cggcgggcaa accatcgaag
    11941 tgcaatttcg cgatctcatt atcgacagca ttgatggcaa ttatggtcgt cagaaagtgg
    12001 actacgaact gagctgcgac tcggatgtcc gcgatcccga gtggaatatg acactctcct
    12061 ggacggggac tgccacctcc tacaacgact ctgctattga gacggatgta ccgggcttcg
    12121 gaattgagct tcagcatgac ggtcagcgtt ttaagctcaa cgagccgctt tccattaacg
    12181 ccaccgattt tagccagaag ccaaagctgg aggcggtacc ggtcaaagcg gctgatgcgg
    12241 tgctgacgga cacgaccttt tctgcttacg caaccttacg ggtggattac caatgaaaac
    12301 agcacacatt tattcacccg gttatgcgct gttagcaggc ctgctgctga ccgccagcac
    12361 aggagcacag gcagtcgaca acaacctgca tttttacggc aacctgctga gtaaatcctg
    12421 cacgctggtg gtggatggtg ccaacctggc agaaatccat tttcccaccg tcagcaaccg
    12481 ggatttgatg gtatctgggc agtccgcgca tctgccggtt gtgtttaagc tgaaagactg
    12541 caaagggccg gcgggataca acgtcaaagt cacgctgagc ggcaccgaag acagcggtca
    12601 gccgggtttt ctggcagtcg atgacacctc caccgcgcag ggcgtgggga tagggatgga
    12661 aacgctcgac ggcaagcggg tggatattaa caacaccacc ggggcgacct ttgcgcttac
    12721 caacggcaac aatgatatta atttccgggc ctggatctag gcgaaaagcg gtcgtgatgt
    12781 aaccatcggt gatttcaccg ccagcctgac agcaactttt gagtatattt aaaatgaaaa
    12841 tccttcgtca tgttttatgt cttttattgc tgttaatgtt caatacatgc ggccaggcgg
    12901 cgacgaaagt tggtgagtta tatgtgccag ctggtgccag ttcacctgcg ac
//
LOCUS       DADLTD010000119        12914 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLTD010000119 DADLTD010000000
VERSION     DADLTD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.119"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..880)
                     /gene="rarD"
                     /locus_tag="JN701_002652"
     CDS             complement(<1..880)
                     /gene="rarD"
                     /locus_tag="JN701_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY7365059.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRR"
     gene            complement(932..1399)
                     /locus_tag="JN701_002653"
     CDS             complement(932..1399)
                     /locus_tag="JN701_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY7365060.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIESALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1564..2433
                     /gene="pldA"
                     /locus_tag="JN701_002654"
     CDS             1564..2433
                     /gene="pldA"
                     /locus_tag="JN701_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY7365061.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2566..4395
                     /gene="recQ"
                     /locus_tag="JN701_002655"
     CDS             2566..4395
                     /gene="recQ"
                     /locus_tag="JN701_002655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY7365062.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4459..5079
                     /gene="rhtC"
                     /locus_tag="JN701_002656"
     CDS             4459..5079
                     /gene="rhtC"
                     /locus_tag="JN701_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY7365063.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5141..5761)
                     /gene="rhtB"
                     /locus_tag="JN701_002657"
     CDS             complement(5141..5761)
                     /gene="rhtB"
                     /locus_tag="JN701_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY7365064.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5872..6894
                     /gene="pldB"
                     /locus_tag="JN701_002658"
     CDS             5872..6894
                     /gene="pldB"
                     /locus_tag="JN701_002658"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY7365065.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6902..7702
                     /gene="yigL"
                     /locus_tag="JN701_002659"
     CDS             6902..7702
                     /gene="yigL"
                     /locus_tag="JN701_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY7365066.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7778..8677
                     /gene="bioP"
                     /locus_tag="JN701_002660"
     CDS             7778..8677
                     /gene="bioP"
                     /locus_tag="JN701_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY7365067.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8565..9518)
                     /gene="metR"
                     /locus_tag="JN701_002661"
     CDS             complement(8565..9518)
                     /gene="metR"
                     /locus_tag="JN701_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY7365068.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9754..12015
                     /gene="metE"
                     /locus_tag="JN701_002662"
     CDS             9754..12015
                     /gene="metE"
                     /locus_tag="JN701_002662"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY7365069.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12300..12545)
                     /locus_tag="JN701_002663"
     CDS             complement(12300..12545)
                     /locus_tag="JN701_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY7365070.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12535..>12914)
                     /locus_tag="JN701_002664"
     CDS             complement(12535..>12914)
                     /locus_tag="JN701_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365071.1"
                     /translation="FNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPINEVM
                     INRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTAT
                     LEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         3051 a         3260 c         3441 g         3162 t
ORIGIN      
        1 ttctacgctg cgtataaatc gcatccatca caaaaattgc cagcgccacc caaataaagg
       61 cgaaggtcac catcttatcg gcacccggtt tttcaccata aaacgtcact gccaacagga
      121 acatcagcgt cgggccaata tactggaaga agcctaacgt ggagagacgc aggcgtgtag
      181 cagctgcggt aaaacacaac agcggtacgg tggtgacaat accggcggcg atcagcagca
      241 aattcagcaa catcgggttt tgccccatat ggctggttga gctgtcggca atagcaaaca
      301 gataaatcgc cgccacgggc agcagccaca tggtttcgat taacatgccg gtttgcgctt
      361 caacggcaat tttcttgcgt accagtccgt agaaggcaaa actaaacgcc agccccagcg
      421 cgatgattgg cagcgaacca aaagtccaca actgaaccaa cacgccacat atcgccagaa
      481 tcaccgccag ccattgcatc cggcggaatc gctcgccgag gaaaatcatc cccagcacaa
      541 tgttcaccag cgggttaata aagtaaccaa ggctcgcttc cagcatatgg tgattgttca
      601 ccgcccagat aaacagtagc cagttgccac caatcagcac ggcagagact gccagcataa
      661 aaattttctg tggcgtctga atcagcgttt ttaaatagga ccactggcgg caaatgctca
      721 tcagcaccac cataaagaaa aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg
      781 gcacgtagta aatcagcttg aagtatgctg gcgcaatacc ccaaataaaa taagcggcaa
      841 gagcgagtaa tacgccctgc cgcgtttgtt ttgcatccat cgggaatact catttttaat
      901 tggtaacagc agtttacctg cttttatgtc ttcaacctac catataggtg gcggtggcac
      961 tggcaatata aagctgttct tcattgtgta attcaacgcg ggcgacggcg actttattgc
     1021 ctgcacgcaa caggctactg gtagcagtaa aacgctcgcc cctgcctggg cgcagataat
     1081 caacgcgaag atcaatggtc cccatccgcg atagccgctg gcgtagttca tcttcactga
     1141 tggtttcgtg gcgggttaag gtacttccca cgcacaccag accggcggcg acatccagcg
     1201 ccgactcaat gactccgccg tgcaaaatgc tttgcgccca gttacccacc atcatcggct
     1261 gatttttaaa ggccagctgt gcgaactctt tttcgtaacg ctccagctcc atccctaacg
     1321 cgcggttaaa tggcatgtga taaacaaaca tctcacccac taatttcagg gcttgttcag
     1381 cggtcagtac ggcagacata tcatccttac acttcattgg ttaatgaaat gttgatttta
     1441 tgcttctttg ttgttggttt ctactttagg aagggataac taacggcaat ggagttaagt
     1501 atgtaaaata gcccgcagaa aaacattcac cttatcaata attcgttacg gagaacacga
     1561 ccgatgcgga ctctgcaggg ctggttgttg ccggtgttta tgttgcctat ggcagtatat
     1621 gcacaagagg caacggtgaa agaggtgcat gacgcgccag cggtgcgtgg cagtattatc
     1681 gccaatatgc tgcaggagca tgacaatccg ttcacgctct atccttatga caccaactac
     1741 ctcatctaca cccaaaccag cgatctgaac aaagaagcga ttgccagtta cgactgggcg
     1801 gaaaatgcgc gtaaggatga agtaaagttt cagttgagcc tggcatttcc actgtggcgt
     1861 gggattttag gcccgaactc ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa
     1921 ctgtccaata gcgaagagtc ttcaccgttt cgtgaaacca actacgaacc gcaattgttc
     1981 ctcggttttg ccaccgatta ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg
     2041 tataaccacg actctaacgg gcgttccgac ccgacctccc gcagctggaa ccgcctttat
     2101 actcgcctga tggcagaaaa cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg
     2161 ggtaatactg acgataaccc ggatatcacc aaatatatgg gttactacca gcttaaaatc
     2221 ggctatcacc tcggtgatgc ggtgttgagt gcgaaaggac agtacaactg gaataccggt
     2281 tacggcggcg cggagttagg cttaagttac ccgatcacca aacatgtgcg cctttatact
     2341 caggtttaca gcggctatgg cgaatcgctc atcgactata acttcaacca gacccgtgtc
     2401 ggtgtaggag ttatgctaaa cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg
     2461 gaacccagca tccaggatta ccctctcaga gactaaaagc attgcagttt ctcgcgcagg
     2521 cgctgaaaat agcgcctgtt tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa
     2581 gtgttgaatc tggagtccgg agctaaacag gttttacaag aaacctttgg ctaccaacag
     2641 tttcgccccg gccaggaaga aattatcgac actgtgcttt ccggtcgcga ttgcctggtc
     2701 gtcatgccca ccggtggcgg aaaatccctt tgctatcaaa ttcctgcctt attgctaaac
     2761 ggccttaccg tggttgtttc accgctgatt tcgttgatga aagatcaggt ggatcaactg
     2821 caagccaacg gcgtggcggc ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt
     2881 gaagtgatga caggctgccg caccgggcaa attcgtctgc tttatatcgc cccggaacgc
     2941 ctgatgctgg ataactttct tgagcatctg gcgcactgga atccggtgtt attagccgtt
     3001 gatgaagcgc actgtatctc ccaatggggc cacgatttcc gcccggaata tgccgcgctt
     3061 ggtcagttac gccagcgttt cccgaccctg ccgtttatgg cgctgactgc cacagccgac
     3121 gacaccacgc gccaggatat cgtgcgcttg ctggggctga acgatccgct gattcaaatc
     3181 agcagttttg accgtccgaa tattcgctac atgctgatgg agaagttcaa accgctcgat
     3241 cagttgatgc gctacgtgca ggaacagcgc ggtaagtcag gcattatcta ctgcaacagc
     3301 cgcgcgaaag tagaagacac cgctgcgcgc ctgcaaagca agggaattag cgcggcggcc
     3361 tatcatgctg ggctggaaaa taatgttcgc gccgacgtgc aggaaaaatt tcagcgcgat
     3421 gacctgcaaa ttgtggtggc gacggtggcg ttcggcatgg gcatcaataa accaaacgtt
     3481 cgcttcgtgg tccactttga tattccgcgc aatatcgaat cctattatca ggaaaccggt
     3541 cgcgccgggc gtgatggcct gcccgcggaa gcgatgctgt tttacgatcc ggccgatatg
     3601 gcgtggctgc gccgttgcct ggaagagaag ccgcaggggc agttacagga tatcgaacgt
     3661 cataaactca atgcgatggg cgcttttgcc gaagcgcaaa cttgccgtcg tctggtattg
     3721 ctgaactatt ttggcgaagg gcggcaagag ccgtgcggga actgcgatat ctgcctcgat
     3781 ccgccgaaac agtacgacgg ttcaaccgat gctcagattg ccctttccac cattggtcgt
     3841 gtgaatcagc ggtttgggat gggttatgtg gtggaagtga ttcgtggtgc taataaccag
     3901 cgtatccgcg actatggtca tgacaaactg aaagtctatg gcatgggccg tgataaaagc
     3961 catgaacatt gggtgagcgt gatccgccag ctgattcacc tcggcctggt gacgcaaaat
     4021 attgcccagc attctgccct acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa
     4081 tcctctttgc aacttgccgt gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa
     4141 tcgttcggcg gcaactatga tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg
     4201 attgccgatg aaagtaatgt cccgccgtac gtggtgttta acgacgcaac cttgattgag
     4261 atggctgaac agatgccgat caccgccagc gaaatgctca gcgttaacgg cgttgggatg
     4321 tgcaagctgg aacgctttgg taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc
     4381 gacgacgaag agtagtcagc agcataaaaa agtgccagga tgaagactcc gtaaacgttt
     4441 cccccgcgag tcaaatgtat gttgatgtta tttctcaccg tcgccatggt gcacattgtg
     4501 gcgcttatga gccccggtcc cgatttcttt tttgtctctc agaccgctgt cagtcgttcc
     4561 cgtaaagaag cgatgatggg cgtgctgggt attacctgcg gcgttatggt ttgggcaggg
     4621 attgcgttgc ttggcctgca tttgattatc gaaaaaatgg cctggctgca tacgctgatt
     4681 atggttggcg gtggcctgta tctctgctgg atgggttacc agatgctacg cggtgcactg
     4741 aaaaaagagg ctgtatctgc gcctgcacca cagattgagc tggcgaaaag cgggcgcagt
     4801 ttcctgaaag gtttactgac caatctcgct aatccgaaag cgattatcta ctttggctcg
     4861 gtgttctcat tgtttgtcgg tgataacgtt ggcactaccg cgcgctgggg catttttgcg
     4921 ctgatcattg tcgaaacact ggcgtggttt accgtcgttg ccagcctgtt tgccctgccg
     4981 caaatgcgcc gtggttatca acgtctggcg aagtggattg atggttttgc cggggcgtta
     5041 tttgccggat ttggcattca tttgattatt tcgcggtgat gccagacgcg tcttcagagt
     5101 aagtcggata aggcgtttac gccgcatccg acattatttt tcacgcatgc ctcgccgatg
     5161 ctaacagcgc tcccaccagc ataaacaacg agccgaaaat cttattcagc gccttcatct
     5221 gctttggtcc tttaatccat agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca
     5281 caataatatc gactacaata gtggtgacgc cgagcacgat atactgcatc agttgcggct
     5341 gctgtggcat gatgaattgc ggaaatagcg ccgccagaaa cacaatactt ttgggattgg
     5401 tgagattcac aaaaactgcg cgctggaaca aatgtcgacg cgattgagta gaggccagcg
     5461 atttaaggtc aattgcacca gcggcgcgcc actgctggat tcccagccaa atcaagtaag
     5521 ccgcgcctgc ccacttcaac acttcaaacg caatcactga gcgggaaaat agcgtcccca
     5581 aacccacgcc aaccagcaca atatgaatcg ccagtccggt ctgaagccca gcaatagacg
     5641 ccaccgcgcc gcgataaccg tggttgagcg aggtggtcat agtgttgatt gcaccagagc
     5701 ctggcgacag gcttaaaatg atcgatgtca gcaggtaggc aaaccaccat tctaaggtca
     5761 tgatgaactc ccggtgtgtc tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt
     5821 caggcacgct aaaaaaaacg attttacgtg gtttaagagg cagattaccc gatgtttcag
     5881 cagcaaaaag actgggaaac aagagaaaac gcgtttgctg cttttaccat gggaccgctg
     5941 actgatttct ggcgtcagcg tgatgaagca gagtttactg gtgtggatga cattccggtg
     6001 cgctttgtcc gttttcgcgc acagcaccat gaccgggtgg tagtcatctg cccggggcgt
     6061 attgagagct acgtaaaata tgcggaactg gcctatgacc tgttccattt ggggtttgat
     6121 gtcttaatca tcgaccatcg cgggcaggga cgttccggtc gcctgttagc cgatccgcat
     6181 ctcgggcatg ttaatcgctt taatgattat gttgatgatc tggcggcatt ctggcagcag
     6241 gaggtccagc ccggtccgtg gcgtaaacgc tatatactgg cacattcgat gggcggtgcg
     6301 atctccacat tatttctgca acgccatcca ggtgtatgtg acgccattgc gctaactgcg
     6361 ccaatgtttg ggatcgtgat tcgtatgccg tcatttatgg cacggcagat cctcaactgg
     6421 gccgaagcgc atccacgttt ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg
     6481 ttgccgtttg ctatcaacgt actgacccac agcagacagc gatatcgacg taacttacgc
     6541 ttctatgctg atgacccaac gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa
     6601 agtattctgg ctggcgaaca ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt
     6661 ctcttgcagg ctgaagagga acgcgtggtg gataaccgca tgcatgaccg tttttgtgaa
     6721 ctccgcaccg ccgcgggcca tcctgtcgaa ggaggacggc cgttggtaat taaaggtgct
     6781 taccatgaga tcctttttga aaaggacgca atgcgctcag tcgcgctcca cgccatcgtt
     6841 gattttttca acaggcataa ctcacccagc ggaaaccgct ctacagaggt ttaaatttct
     6901 tatgtaccag gttgttgcgt ctgatttaga tggcacgtta ctttctcccg accatacgtt
     6961 atccccttac gccaaagaaa ctctgaagct gctcaccgcg cgcggcatca actttgtgtt
     7021 tgcgaccggt cgtcaccacg ttgatgtggg gcaaattcgc gataatctgg agattaagtc
     7081 ttacatgatt acctccaatg gtgcgcgcgt tcacgatctg gatggtaatc tgatttttgc
     7141 tcataacctg gatcgcgaca ttgccagcga tctgtttggc gtagtcaacg acaatccgga
     7201 catcattact aacgtttatc gcgacgacga atggtttatg aatcgccatc gcccggaaga
     7261 gatgcgcttt tttaaagaag cggtgttcaa atatgcgctg tatgagcctg gattactgga
     7321 gccggaaggc gtcagcaaag tgttcttcac ctgcgattcc catgaacaac tgctgccgct
     7381 ggagcaggcg attaacgctc gttggggcga tcgcgtcaac gtcagtttct ctaccttaac
     7441 ctgtctggaa gtgatggcgg gcggcgtttc aaaaggccat gcgctggaag cggtggcgaa
     7501 gaaactgggc tacagcctga aggattgtat tgcgtttggt gacgggatga acgacgccga
     7561 aatgctgtcg atggcgggga aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga
     7621 ccttcatccc gagctggaag tgattggtac taatgccgac gacgcggtgc cgcattatct
     7681 gcgtaaactc tatttatcgt aatcgttctt tatttggtca gttgtcaacc tgatacttcg
     7741 ctacaatgga tactcgttaa tcaaagagtt ttccattgtg gcgctactta tcatcaccac
     7801 gattctgtgg gccttctcct ttagctttta tggcgagtac cttgcggggc acgtcgatag
     7861 ctattttgcg gtgctggtgc gcgttggcct ggcggcactc gtttttctgc cgtttctgcg
     7921 tacccgtggc aatagcctga aaacagtcgg cctgtatatg ctggtgggcg cgatgcagct
     7981 cggcgtgatg tatatgctga gtttccgcgc ttatctctat ctgacggttt ccgaactgct
     8041 gctgttcacc gtactgacgc cgctctacat cacgctgatt tatgacatca tgaataagcg
     8101 ccgtctgcgc tggggatatg cttttagtgc cttgttggcg gtgattggtg ccgggattat
     8161 tcgctatgat caggtcaccg accatttctg gactggcttg ttgctggtgc aactctccaa
     8221 tatcactttt gccatcggca tggtgggtta caaacgcctg atggaaactc gcccgatgcc
     8281 gcagcataac gcctttgcgt ggttctatct tggcgcgttc ctggtggcgg taattgcatg
     8341 gttcttgctg ggaaatgcgc agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt
     8401 gtttcttggc gtggtggctt ccgggattgg ctactttatg tggaactacg gcgcgacgca
     8461 ggtggacgcc ggaacgctgg gaattatgaa taatatgcac gttccggcag ggctgctggt
     8521 aaacctggct atctggcacc aacagccgca ctggccaacg ttgattacag gcgcgctggt
     8581 gatcctggcc tcactgtggg tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc
     8641 agatgatcgc aggcgtgatt gcgcgctgag cgaataaacg cttccgtaac tggctgacgc
     8701 tgctcgccat cgcgcacggc ggcgtacagt cggctccaca agccttcgcc cagggttttg
     8761 gtcaccacca gaccctggcg ctcaaaactc tctactaccc aatgcggtag cgcggcaata
     8821 cccatccgcg cggcaaccat ctgaatcaac aataaggtgt tatcgacgct tttcagtgac
     8881 ggactgacgc ctgccggctg aagaaaatgc cgccagacat ccagtcgact acgctgcacc
     8941 ggataaatta atagcgtctc gctggcgaga tcttccggtg taatacgcgt ttttgccgcc
     9001 agtggatggt caggtgctaa caccagacgc acttcatagt cgaacatcgg cgaataatgc
     9061 agaccactgc gcggcagaat atcggacgtc attaccagat ccagctctcc ctgttgcaag
     9121 gcgggctgcg ggtcaaatgt cacgcccgat ttaaagtcca tctcgacctg cggccagttc
     9181 ttatggaaat tttctaacgc gggcgtcagc cactgtatac agctatggca ctcaatggca
     9241 atgcgcagac gcgtctgctg cggttcattg caggcttgca gtgcctggct aatttgtggc
     9301 agcacctggt ttgccagttg caacaggatt tctccctgcg gtgtaaagcg aagcggctgg
     9361 cttttacgca caaatagccg gaagccaagg cgttgttcca gatcgctaaa ctggtgagac
     9421 agggcggatt gcgtttgatg caacgtcgcc gcagcggctg cgagtgagcc gcagttccgc
     9481 aacgcttgta gcgttttcag atgttttact tcgatcatga aagtccttca cttcggcatg
     9541 aataatttgc gcttgaggaa tatacagtaa ccgccaatta tggatgtgta aacatctgga
     9601 cggctaaaat ccttcgtctt ttaaatttat ggtgcgttgg ctgcgtttct ccaccccggt
     9661 cacttacttc agtaagctcc cggggatgaa taaacttgcc gccttcccta aattcaaaat
     9721 ccataggatt tacatataat tagaggaaga aaaatgacaa tattgaatca caccctcggt
     9781 ttccctcgcg ttggcctgcg tcgcgagctg aaaaaagcgc aagagagtta ttgggcaggg
     9841 aactccacgc gtgaagaact gctggcggta gggcgtgaat tgcgtgctcg tcactgggat
     9901 caacaaaagc aagcgggtat cgacctgctg ccggtgggcg attttgcctg gtacgatcat
     9961 gtactgacca ccagtctgct gctgggtaac gttccggcgc gtcatcagaa caaagatggt
    10021 tcggtagata ttgacaccct gttccgtatt ggtcgtggac gtgcgccgac tggcgaacct
    10081 gcggcggcag cggaaatgac caaatggttt aacaccaact atcactacat ggtgccggaa
    10141 ttcgttaaag gccaacagtt caaactgacc tggacgcagc tgctggagga agtggacgag
    10201 gcgctggcgc tgggccacaa cgttaagcct gtgctgctgg ggccggttac ctggctgtgg
    10261 ctggggaaag tgaaaggtga acagtttgac cgcctgagcc tgctgaacga cattctgccg
    10321 gtttatcagc aagtgctggc agaactggag aaacgcggca tcgagtgggt acagattgat
    10381 gaacccgcgc tggtactgga actaccacag gcgtggctgg acgcatacaa acccgcttac
    10441 gacgcgctcc agggacaggt gaaactgctg ctgactacct attttgaagg cgttacgcca
    10501 aatctcgaca cgattactgc gctgcctgtt cagggactgc atgtggacct tgtacatggt
    10561 aaagatggcg ttgctgaact gcacaagcgc ctgccttctg actggctgct gtctgcggga
    10621 ctgatcaatg gtcgtaacgt ctggcgcgcc gatcttactg agaaatatgc gcaaattaag
    10681 gacattgtcg gcaaacgtga tttgtgggtg gcatcttcct gctcgttgct gcacagcccc
    10741 atcgacctga gcgtggaaac gcgtcttgat gcagaagtga aaagctggtt tgccttcgcc
    10801 ttacaaaaat gccatgaact ggcactgctg cgcgatgcgc tgaacagtgg tgacacggca
    10861 gctctggcag agtggagcgc cccaattcag gcgcgtcgtc actctactcg tgtacataat
    10921 ccggcggtag aaaagcgtct ggcggcgatc accgcccagg acagccagcg tgcgaatgtc
    10981 tatgaagtgc gtgctgaagc ccagcgtgcg cgttttaaac tgccagcgtg gccgaccacc
    11041 acgattggtt ccttcccgca aaccacggaa attcgtaccc tgcgtctgga tttcaaaaag
    11101 ggcaatctcg acgccaacaa ctaccgcacg ggcattgcgg aacatatcaa gcaggccatt
    11161 gttgagcagg aacgtttggg actggatgtg ctggtacatg gcgaggccga gcgtaatgac
    11221 atggtggaat actttggcga gcacctcgac ggatttgtct ttacgcaaaa cggttgggta
    11281 cagagctacg gttcccgctg cgtgaagcca ccgattgtta ttggtgacgt tagtcgcccg
    11341 gcaccgatta ccgtggagtg ggcgaagtat gcgcaatctc tgactgataa accggtgaaa
    11401 gggatgctga ctggcccggt gaccattctc tgctggtcgt tcccgcgtga agatgtcagc
    11461 cgtgaaacca tcgccaaaca aattgcgctg gcgctgcgtg atgaagtggc ggacctggaa
    11521 gccgcaggaa ttggcatcat ccagattgat gaaccggcgc tgcgcgaagg tttaccgctg
    11581 cgtcgtagcg actgggatgc atatctccag tggggcgtgg aggccttccg tatcaacgcc
    11641 gccgtggcga aagatgacac acaaatccac actcacatgt gttattgcga gttcaacgac
    11701 atcatggatt ccattgcggc actggatgca gacgtcatca ccatcgaaac ctcgcgttcc
    11761 gacatggagt tgctggagtc gtttgaagag tttgattatc cgaatgaaat cggtccaggc
    11821 gtctatgaca ttcactcgcc aaacgtaccg agcgtagaat ggattgaagc cttgctgaag
    11881 aaagcggcaa aacgcattcc ggcagagcgc ctgtgggtca acccggactg tggcctgaaa
    11941 acgcgcggct ggccagaaac tcgcgcggca ctggcgaaca tggtgcaggc ggcgcagaat
    12001 ttgcgtcggg gatgatgaat attaacgagt cactgtaaat tgcagtacga tgaaagaggc
    12061 aaaactgcct gataccaggc ctacataatt tatgcaattt attgaatttt tgagttttgt
    12121 tggccggaca aggcgttcac gccgcatccg gcatgaacaa cgcgcacttt gccaataatc
    12181 tgatggcttc catcaccgga agccattttg ctatctgcgc ccgttcagat acagaaataa
    12241 ccgaatcatt tttacgcaaa atataatcct gagcgatttg ttcagtaatc aacgctacgt
    12301 tatttattct cacttttcac gaaattgtat cccttttcag taacgtggta gtgaggcata
    12361 gacttatgtc cgctatcctc aataataacc aaaccctcgt tagctaattt gttaacaatg
    12421 ttaacaacat taccgaatcc tggaatttgc cttatggata gtgtgcgatc gagattcatc
    12481 cagttccagt ttatatcttt ctcagccata actttcagta cgttaatttc agtttcagtt
    12541 atcatagtca agtgcatcct ggtggtataa gtcattaagt tctcccttaa gtttataatc
    12601 ttctagagta gcagtatgcg tatgattcca gagtgcatag cctgcgtcac catcaggttg
    12661 tcctgtttcc caaccaagat tacagtaacg ctgatattcc cgacactcat gagtatagaa
    12721 gttaagatct acttgttctg gggggatttt tcctttctca atgtctttca gtctattgat
    12781 catcacttca tttatagggt catgaccaaa tctggctatg tgtttttcaa caatagcgat
    12841 accttccctg ttgattgtta cgccatctgt cgtcagattt tctattggac caccggcaag
    12901 gtcgggatta aatg
//
LOCUS       DADLTD010000120        12908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLTD010000120 DADLTD010000000
VERSION     DADLTD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.120"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            13..1566
                     /locus_tag="JN701_002665"
     CDS             13..1566
                     /locus_tag="JN701_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7365072.1"
                     /translation="MSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDL
                     CTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKIS
                     KDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENL
                     HALKEKRLNGMPKTTPKSGQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSIT
                     PPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFS
                     LLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNEFLFNHQ
                     QDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQL
                     YNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
                     TWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGKT
                     GEPPNYLYL"
     gene            1902..2879
                     /gene="csiD"
                     /locus_tag="JN701_002666"
     CDS             1902..2879
                     /gene="csiD"
                     /locus_tag="JN701_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAY7365073.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2899..4167
                     /gene="lhgO"
                     /locus_tag="JN701_002667"
     CDS             2899..4167
                     /gene="lhgO"
                     /locus_tag="JN701_002667"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAY7365074.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            4190..5625
                     /gene="gabD"
                     /locus_tag="JN701_002668"
                     /pseudo
     CDS             4190..5625
                     /gene="gabD"
                     /locus_tag="JN701_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            5639..6905
                     /gene="gabT"
                     /locus_tag="JN701_002669"
                     /pseudo
     CDS             5639..6905
                     /gene="gabT"
                     /locus_tag="JN701_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7143..8504
                     /gene="gabP"
                     /locus_tag="JN701_002670"
     CDS             7143..8504
                     /gene="gabP"
                     /locus_tag="JN701_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY7365075.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            8563..9225
                     /gene="csiR"
                     /locus_tag="JN701_002671"
     CDS             8563..9225
                     /gene="csiR"
                     /locus_tag="JN701_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY7365076.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9226..9675)
                     /gene="kbp"
                     /locus_tag="JN701_002672"
     CDS             complement(9226..9675)
                     /gene="kbp"
                     /locus_tag="JN701_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY7365077.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9759..9917)
                     /locus_tag="JN701_002673"
     CDS             complement(9759..9917)
                     /locus_tag="JN701_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY7365078.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10100..10399
                     /locus_tag="JN701_002674"
     CDS             10100..10399
                     /locus_tag="JN701_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7365079.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10409..10933
                     /gene="ygaP"
                     /locus_tag="JN701_002675"
     CDS             10409..10933
                     /gene="ygaP"
                     /locus_tag="JN701_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY7365080.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(10980..11384)
                     /gene="stpA"
                     /locus_tag="JN701_002676"
     CDS             complement(10980..11384)
                     /gene="stpA"
                     /locus_tag="JN701_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY7365081.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMEADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            12051..12500
                     /gene="alaE"
                     /locus_tag="JN701_002677"
     CDS             12051..12500
                     /gene="alaE"
                     /locus_tag="JN701_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY7365082.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(12537..12881)
                     /locus_tag="JN701_002678"
     CDS             complement(12537..12881)
                     /locus_tag="JN701_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY7365083.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         3217 a         3163 c         3296 g         3232 t
ORIGIN      
        1 atcagggaaa atatgtctga gtttgcacgt tgccattatg ggtatataca aatccctcca
       61 gtgacaactt tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt
      121 catgcttatc aacccgaaga tctttgtacc atccataacc caatgggaga tttgcaggat
      181 tttatcgcat tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattataca
      241 tttaacttta tgggaatcgg gggtagtggt aaaaatgacc tggattatcc ctctgctgat
      301 atacgagcga agatcagtaa agatatagaa agcggtatcc ctggctattg gcaaggtcag
      361 gttttgattc cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa
      421 gatatacatc tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa
      481 tgggaaaacc tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa aacaacaccc
      541 aaaagtggcc aggttattat gttgcaaaat caatacgttc gagaaatgcg aaaatatggc
      601 gtacgaggtt tacgttatga tgcggcaaaa cactcaaaac atgaacaaat agagaggtcg
      661 ataaccccac cgcttaaaaa ttataatgag cggttacaca atactaattt atttaaccca
      721 aaatatcaaa aaaaagccgt tatgaattac atggaatacc tggtaacttg tcagttggat
      781 gaacaacaaa tgtcatcact gctttatgag agagatgatt taagtgccat tgatttttct
      841 ttactcatga aaacgataaa agccttttca tttggtggag atctccaaac ccttgcatca
      901 aaaccgggtt ccacaatctc aagtatccca tcagaaagac ggatattgat taacattaac
      961 cacgattttc ctaacaatgg taatcttttc aatgagtttc tatttaacca tcaacaagat
     1021 gaacaattag caatggcata tatggccgct ctcccgttca gcagaccttt agtttactgg
     1081 gatggccaag tattaaaatc aacgactgaa attaaaaatt atgatgggtc cacgcgtgtc
     1141 ggcggtgagg cgtggcttaa taaaggttgc tctacctatc agcagctcta caatgaattc
     1201 cacgcattat atatagataa agcaggaata tggagcgcat ttgagggtgt atttgcaact
     1261 aaaaacgttc tggcctttag tcgtggggat tctgtgaaca ttaatcactc tcctcatgat
     1321 gggctagtta taataaataa aggaaacgaa gaagttgaag gtacctggcc taacaaattg
     1381 caacctggaa tatacaaaaa catggggagt aatagcgtta acattattat taataatacc
     1441 cgaaaaatta tcccccctgg taaagtattt acgcttagag gcggaactct aaatatcaat
     1501 attcctgggc gtagcgctct tcttttaggg aaaacaggag aaccgccgaa ctatctctat
     1561 ttataattta atgttatatc tgccccgata aaacggggca gataatatgt ttagtttact
     1621 aacggtcatt ttgcagtgaa gccatttact gttttttatc gaccagataa tctgttctct
     1681 aatgttaact ccccctaacc tgttgcttta gttattcatt tcctgtctca ctttgcctta
     1741 ataccctacg ttaaatgtta ctaatttgtt gtttttgatc acaataagaa aacaatatgt
     1801 cgcttttgtg cgcatttttc agaaatgtag atatttttag attatggcta cgaaatgagc
     1861 atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca ctgatcgccg
     1921 tacaaaataa cgctgtcgat tcaggccagg actatagcgg attcactctc atcccgtcgg
     1981 cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa cagtttctcg
     2041 aacaggttgc cgaatggccc gtgcaggcgc tggagtacaa atcgtttctg cgttttcggg
     2101 taggcaaaat tcttgacgat ctgtgtgcga atcagctgca accattgctg ttgaagcccc
     2161 tgctaaaccg tgctgaaggt gcgctgttga tcaatgcggt gggtatcgat gatgtggcgc
     2221 aggcagatga gatggtgaag ctggcaacgg cggtagcgca tctgattggt cgctcaaatt
     2281 tcgatgcgat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtc gataactcag
     2341 acagctatct gcgtcagccg caccgcgtga tggaactgca caacgacggc acgtatgtcg
     2401 aagagatcac cgattacgtg ctgatgatga aaatcgatga gcaaaacatg cagggtggaa
     2461 attcgttgct gctgcatctc gatgactggg aacatctgga ccactatttc cgccatccac
     2521 tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt
     2581 tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat atcgaccagt
     2641 tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc gatgccattg
     2701 aaaccagcaa aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgataaaca
     2761 acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac
     2821 tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag
     2881 cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg
     2941 tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa
     3001 gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggggtc
     3061 tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cgggaaaccg cgccaccaaa
     3121 gccttttgcg atcaaaacga cattcgctat gacaactgcg gcaagatgct gatcgccacg
     3181 tccgatctcg aaatggaacg gatgcgcgcg ttgtgggaac gcacggcggc gaacggtatc
     3241 gagcgcgagt ggttaaacgc cgaggaactg cgcgagcgcg aaccgaatat caccgggctc
     3301 ggcggcattt ttgtgccgtc cagcggcatt gtcagctatc gcgaagtcac agcggcaatg
     3361 gcaaaaatct tccaggccag aggcggtgag attatttata acgccgaagt cagcgccctc
     3421 agtgagcata aaaacggcgt ggtcgtccga acccgtcagg gcggcgaata tgaagcatcg
     3481 acgctgatta gctgttccgg gctgatggct aaccggctgg tgaaaatgct cggcctcgaa
     3541 ccgggtttta ttatctgccc gttccgtggc gagtatttcc gccttgcgcc ggagcataac
     3601 cagattgtta accatctgat ttaccccatc cctgacccgg caatgccgtt tttgggcgtg
     3661 catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt gctggctttt
     3721 aaacgcgaag gctatcgcaa gcgcgacttc tcatttagcg acacgctgga aattttaggc
     3781 tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa
     3841 aactcgctgt gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg
     3901 ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc aggcggtatc gccggacggc
     3961 aagctgattg acgattttct gtttgtcacc accccgcgca cgatccacac ctgcaatgcg
     4021 ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa
     4081 acgctgttgg caagccagag taaccccgga cgcacgctgc gagcgacacg tagtgtggat
     4141 gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa
     4201 cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa
     4261 tggcgggatc atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa
     4321 aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc tgcccgccag
     4381 gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca atttgatgat
     4441 ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggcaaac cactggccga
     4501 agcgaaaggc gaaatcagct acgctgcctc ttttattgag tggtttgccg aagaaggcaa
     4561 acgtatttat ggcgatacca ttcccggtca tcaggccgat aagcgcctga ttgttatcaa
     4621 gtagccgatt ggcgttaccg cagctatcac gccgtggaac ttcccggcgg cgatgattac
     4681 ccgtaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca
     4741 gacaccgttc tctgcgctgg cgctggcgga gctggcgatt cgcgcgggca ttgcggctgg
     4801 cgtgtttaac gtggtcaccg gttcggcagg cgcggtcggt aatgaactga ccagcaaccc
     4861 gctggtacgc aaactgtcgt ttaccggttc gaccgaaatt ggccgacagt taatggaaca
     4921 gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt
     4981 ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa
     5041 cgccgggcaa acctgcgtct gcgccaaccg tttggcgtgt atgaccgctt tgccgaaaaa
     5101 ttgcagcagg cggtgagcaa actgcacatc ggcgacgggc tggataaagg cgtcaccatc
     5161 gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg
     5221 gagaaaggtg cgcgtgtggt ttgcggcgct aaagcggatg aacgcggcgg caacttcttc
     5281 cagccgacca ttctggtgga cgtgccggcc aacgccaaag tgtcgaaaga agagacgttc
     5341 ggccccctcg ccccgctgtt ccgctttaaa gatgaagccg atgtgattgc gcaagccaat
     5401 gacaccgagt ttggccttgc cgcctatttc tacgcccgtg atttaagccg cgtcttccgc
     5461 gtgggcgaag cgctggagta cggcatcgtc ggcatcaata ccggcattat ttccaatgaa
     5521 gtggccccgt tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc
     5581 atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga gaatgcgaat
     5641 gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg gcgttgggca
     5701 aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg ttgaaggccg
     5761 tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc tgcatccgaa
     5821 ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaagtgct
     5881 ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt
     5941 cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat
     6001 cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgcgt atcacgggcg
     6061 cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatggggct
     6121 gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca taagcgagga
     6181 tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg aagatatcgc
     6241 cgccatcgtg attgagccgg ttcagggcga aggcggtttc tacgccgcgt cgccagcctt
     6301 tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt
     6361 gcagagcggc gcggggcgta ccggcacgct gtttgcggtg gagcagatgg gcgtggcgcc
     6421 ggatcttacc acctttgcga aatcgatcgc tggcgggcgt caccgggcgc gcggaagtga
     6481 tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg attgcctgcg
     6541 tggctgcgct ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa aaagccaacg
     6601 atctggggca gaagctaaaa gacggattgt tggcgatagc cgaaaaacac ccggagatcg
     6661 gcgacgtacg cgggctggga gcgatgattg ccatcgagct gtttgaagat ggcgatcaca
     6721 gcaaaccgga cgcaaaactc accgccggga tcgtggcacg cgcccgcgat aagggcctga
     6781 ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtactg ctcaccattg
     6841 aagacgctca gatccgtcag ggtctggaga ttatcggtca gtgttttgat gaggcgaagc
     6901 agtagcaccg tccccatgca ggaggcgacg ctgcgcgtct tgtccggcct acgagaatcc
     6961 aggtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg aataaatcta
     7021 tgtcggccgg ataatgcgga gccgctctcc ggcaaaaaga atcaataaca attaaacgcg
     7081 tgacccggcg cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca
     7141 ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaaatca cgccacgtca
     7201 ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca
     7261 tcgccgaagc gggcccggca gtattactgg cctatctgtt cgccgggcta ctggtggtta
     7321 tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt tcgttttcca
     7381 cctatgccga taaagccatt ggtcgctggg cgggctatac catcggctgg ttgtactggt
     7441 ggttttgggt actggttatc ccgctggaag ccaacatcgc cgccatgatc ctgcactcgt
     7501 gggttccagg cattcccatc tggttatttt ccctcgtcat tacccttgcc ttaactggca
     7561 gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgcaaag
     7621 tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt ttttaccctt
     7681 atgccgaagt gagcgggatc tcaagattgt gggatagcgg cggctttatg cccaacggct
     7741 tcggtgcggt attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg
     7801 tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgtccgc gccactaact
     7861 cggttatctg gcgtatttct atcttctatt tgtgctctat ttttgtcgtc gtggcattaa
     7921 ttccgtggaa tatgcccgga ctaaaagccg tcggttctta tcgctcggta ctggaattgc
     7981 tcaatattcc ccatgcgaaa ttaatcatgg actgtgtgat attactttcc gtaaccagct
     8041 gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc cgtcgcggag
     8101 atgcgcctgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac
     8161 tctccaccgg cgcggcattc ttaacggtgg tggtgaacta ttacgtgcct gcgaaggtat
     8221 ttaaatttct gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg
     8281 tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcttgcgca
     8341 tgtggcttta tccctggctc acctggctgg tcatcggctt tattaccttt gtgttggtag
     8401 tgatgctatt ccgcccggcg caacagttag aagtgatctc caccgactta ttagcaatag
     8461 ggattatctg taccgtgcca attatggcgg ctggaataag ctgataatgt ggcaaaaaac
     8521 acccgttcat aatacgcgct gataataatc aggagtcaca ccatgaccat tacgtctctg
     8581 gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc ggatgaaaaa
     8641 ttacggatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt gcgggaagca
     8701 ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg gtatcgggtg
     8761 gcgtctatgt cagagcagga gctgctcgat attttcgatg cccgcgccaa tatggaagcg
     8821 atgttagtga gtctggcgat tgcccgcggt agcgatgagt gggaggcaga cgttctcgca
     8881 aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat gcttgatgag
     8941 tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg ttctcactat
     9001 ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gctatcgatt tatctggctg
     9061 cggcgaacgg tgctttcggt agaaatgctg gaggataaac acgatcagca ccagaccctg
     9121 actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg ccagcattta
     9181 ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact cttccggaat
     9241 acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt tattcgcttc
     9301 gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg cactcagagt
     9361 gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg ttttcacctg
     9421 atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt tctccttcgc
     9481 ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt gaatgttcac
     9541 tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct tcgcctgatc
     9601 gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat ctttcacaaa
     9661 attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag tgtagcagag
     9721 gaacgcggcg cgttaacgcc gcggggggga gcacaggttt aatcgcgggt ttgcacccag
     9781 aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt aataatgaac
     9841 gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat ggtgatgacg
     9901 attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt aaaaagagat
     9961 tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta cgcttttact
    10021 ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt tacttaattt
    10081 agaaagtact taaataaata tgactgaact cgcgcaatta caggccagtg ccgaacaggc
    10141 agcggcctta ttgaaagcaa tgagccatcc taaacgattg ctgattctgt gcatgcttag
    10201 cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga gtgcctctgc
    10261 gacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc aacgggatgc
    10321 ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg ccaccctgaa
    10381 aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc cgcatgatgc
    10441 acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg atgaatatct
    10501 tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac agtcaggtct
    10561 tccacctaaa ttacgtcgcg agcaaattat attccactgc caggcaggta aacgcaccag
    10621 taataacgcc gataaattag cagcgattgc cgcccccgca gaaatatttt tactcgaaga
    10681 taggattgat ggctggaaaa gagctggatt gccagtagcg gtaaataaat ctcaaccctt
    10741 gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg gcgttgtact
    10801 gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg ccgggttact
    10861 gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga tgccgtggaa
    10921 ccaacgagct tgagaagcga cgccggacgc gccctagcag cgacatccgg cctcagtaat
    10981 tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat tggcttcggt
    11041 gtacggccct gaccggtcca ggtcttagtt tcaccgttaa catcagtgaa tttatatttc
    11101 gccggacgcg gctggcgttt tttaccagca cgtggtgcag cagcagagct attacccaat
    11161 aactcttccg ggttaattcc gtcagcttcc atcagctcca gccaggtgct aattttttcc
    11221 tgacgctcag ccagttcacg ctgctgctgt tcttcttctt cacgtctttc tttagtgaca
    11281 accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg agccatcgca
    11341 cggagggttc gaatgttatt taaactttgt aacattacgg acataataaa gaaaaccttt
    11401 taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta ttatatttcc
    11461 acaaaattta atctatttac agtcctgcgc gagattattt cagctgttat tttctgaaac
    11521 tctcagaata atttcaagat tatatattag ccattttgat gagtaattta ggttattggt
    11581 gaacagagtg gcgctctctg ctcgctaact ccgcaagcct gataaaaccg aagtgaataa
    11641 aaatgcaata taacagaata aaagaaaaca cccctcactg gtgctttctg ctgttaatgt
    11701 acattttctg gcatcaaaaa tgctgtaaat gacccggtca cgacaaacac cgaattctct
    11761 acgcttatag cgttacctca cccccaaact ttcattagtg aaattaaagg caaggtcata
    11821 tccaattgat ttaattgaaa ataaatcata tcggcatgta aaaatccaca atgtacaaaa
    11881 aacgagctgt tacggaatat tttatctaca aaaactgact aaataaaagt ttttcactaa
    11941 ttgattagtc atagccagcg atatacgcta tgcgaaaatg cagatggcaa tgagatccac
    12001 tgctttcatc tccattaaca tcccattacg ctttattaag gagcattagc atgttctcac
    12061 cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt tactgttctg
    12121 tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag tctttttatt
    12181 ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt atgtaccgcg
    12241 atctgtttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa aatctggctg
    12301 atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg ttagtggtgg
    12361 gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt tcgatgttga
    12421 tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa gtcagccgtt
    12481 accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc gcgtctttaa
    12541 ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga actgaagcca
    12601 tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa ttgctggtaa
    12661 tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg ttgaactgcg ttcttttaat
    12721 gttggatgaa ttaccagagc ggtacgtccc attcgcgctt cgcgattaac atagacataa
    12781 ttttccccac ggcggtaacc atacgctttc tgggttacca catcgacagt aaaaccgact
    12841 ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc tcgttatttt
    12901 caaccgcg
//
LOCUS       DADLTD010000121        12908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLTD010000121 DADLTD010000000
VERSION     DADLTD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.121"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(75..1613)
                     /locus_tag="JN701_002679"
     CDS             complement(75..1613)
                     /locus_tag="JN701_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAY7365084.1"
                     /translation="MTLCGPGSVSSCGQQAQTRGLKHLFVMADSFLHQAGMTAGLTRS
                     LAVKGIAMTLWPCPVGEPCITDVCAAVAQLRESGCDGVIAFGGGSVLDAAKAVALLVT
                     NPDSTLAEMSETSVLQPRLPLIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVL
                     SDETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSA
                     RLQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFN
                     IPTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEF
                     NRIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPE
                     YLYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYI
                     MGMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDP
                     SLFF"
     gene            complement(1687..2523)
                     /gene="eutJ"
                     /locus_tag="JN701_002680"
     CDS             complement(1687..2523)
                     /gene="eutJ"
                     /locus_tag="JN701_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAY7365085.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(2534..3937)
                     /locus_tag="JN701_002681"
     CDS             complement(2534..3937)
                     /locus_tag="JN701_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAY7365086.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(3949..4236)
                     /gene="eutN"
                     /locus_tag="JN701_002682"
     CDS             complement(3949..4236)
                     /gene="eutN"
                     /locus_tag="JN701_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAY7365087.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(4343..4636)
                     /gene="eutM"
                     /locus_tag="JN701_002683"
     CDS             complement(4343..4636)
                     /gene="eutM"
                     /locus_tag="JN701_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAY7365088.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(4675..5691)
                     /gene="pta"
                     /locus_tag="JN701_002684"
     CDS             complement(4675..5691)
                     /gene="pta"
                     /locus_tag="JN701_002684"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7365089.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLVDIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(5681..6490)
                     /gene="eutT"
                     /locus_tag="JN701_002685"
     CDS             complement(5681..6490)
                     /gene="eutT"
                     /locus_tag="JN701_002685"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAY7365090.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            complement(6487..7188)
                     /gene="eutQ"
                     /locus_tag="JN701_002686"
     CDS             complement(6487..7188)
                     /gene="eutQ"
                     /locus_tag="JN701_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAY7365091.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            complement(7163..7642)
                     /gene="eutP"
                     /locus_tag="JN701_002687"
     CDS             complement(7163..7642)
                     /gene="eutP"
                     /locus_tag="JN701_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY7365092.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(7655..7990)
                     /gene="eutS"
                     /locus_tag="JN701_002688"
     CDS             complement(7655..7990)
                     /gene="eutS"
                     /locus_tag="JN701_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY7365093.1"
                     /translation="MDKERIIQEFVPGKQVTLVHLISHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(8282..10561)
                     /gene="maeB"
                     /locus_tag="JN701_002689"
     CDS             complement(8282..10561)
                     /gene="maeB"
                     /locus_tag="JN701_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY7365094.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            10850..11800
                     /gene="tal"
                     /locus_tag="JN701_002690"
     CDS             10850..11800
                     /gene="tal"
                     /locus_tag="JN701_002690"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7365095.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            11820..>12908
                     /locus_tag="JN701_002691"
     CDS             11820..>12908
                     /locus_tag="JN701_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7365096.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIVFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTQRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT         2880 a         3696 c         3352 g         2980 t
ORIGIN      
        1 tcccattgac gccatcacgc tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg
       61 ttatctccgc gtcatcagaa gaacagtgac ggatcgcccg cccgtttggt caggcgaccg
      121 tttgccataa tgcccatgct ttccagccag cgttcaaact ctggtgacgg gcgcaggttg
      181 agtaactgac gcacagtggc ggtatcgtgg aatgcggtgg tctggtagtt gagcatgatg
      241 tcatcaccca gcggcatccc catgatgtag ttgcagcctg cggtggcgag caggatcatc
      301 aggttttcgt tgaggttctg gtcagcgtca gcgtggttgg tgtagcagca gtcacagccc
      361 atagagatgc cgctcagctt gcccataaag tgatcttcta agccagcacg gataatctgg
      421 cggtcgttgt agagatactc cggcccaata aagccgacca cggtgttgac gataaacgga
      481 tcgtaatgac gcgccagccc atagttacgt gcttccatcg tcacctggtc agcgccgaag
      541 ttagcgccag cggatagcgc agagccttgt ccggtttcga agtagaggca gttttccccg
      601 gcgatacggt tgaactctgc acccactgcg cgcgcttcgt cgagcatcgc cagttccacg
      661 ccaaactctt tcagcccttt ttcgctgcca cagatactct ggaaaatcag tccgccaggc
      721 gcgccgcgac gaatcgcttc gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt
      781 gggatgttga atttgtcgat cacgccataa atggtatcca gcacgcggct taagttttcc
      841 acgtcgtcag ttaccgggtt gacgccgatc accgcatcgc ccaccccgaa ggaaagccct
      901 tcgtagattt gcgcggcgat actttgcacg tcgtcacgag tgtcatttgg ctgcaaacgg
      961 gcgctgaagg tgcccggaat accgatggtg gtattggcct ttttgattac cggcattttc
     1021 ttcgcgccgt agatcaggtc cgcgttggag caaatcttcg ctaccgccgc gaccacttcc
     1081 gaggtcagcc ctttgcgggt aaaggcaatg tcgtccacgc tagtttcatc gctcagcaca
     1141 tactcacgca gttcgctgat gctccagttt ttaatctggt tgtaggccgt ttcgttaaca
     1201 tcgtcctgaa tcagccgcgt cacgcagtca tcttcatagg caatcagcgg caagcgcggt
     1261 tgcagaacgc tggtttctga catctctgcc agcgtgctat ccgggttcgt caccagcaac
     1321 gccacggctt tcgccgcatc cagcaccgag ccgccgccaa atgcgatcac cccatcacag
     1381 cctgactcac gcaactgcgc cacggctgca cacacgtcgg tgatgcacgg ttcgcccacc
     1441 ggacatggcc agagcgtcat ggcgatgcct ttaacagcca ggctgcgcgt cagcccggcg
     1501 gtcatccctg cctgatgcaa aaagctgtct gccatcacga acagatgttt cagcccacgc
     1561 gtttgcgctt gctgtccgca actgctcacc gagcccggac cgcaaagcgt caccggtgga
     1621 acgctaaatg tttttacccg ttgcagattc agggtatcga acgcctgaaa gagcgcggtc
     1681 tgcaattcat tttgcatata gcccctccgc tttctctcgc ccactactgg cgatcgccag
     1741 cggggtcata aacaaactgt gctgcggtaa atgcacctgt aacgccggga attgtttgcg
     1801 aaacagttcc gccacgcccg gttgcataca agagccgccc gccagccata aatcagtaat
     1861 gccctgccct tcaatatgcc gggcgacaat gtccgccatt ttttcgtaca ccggtttcac
     1921 cgtaggccaa atctcttcac cgtgaccgcg cttgtactgc tccgcctctt ccagcgaaat
     1981 acggcgattt ccggcgagag tcagagagat gtgatgtccg ccggttgctt catccgccga
     2041 gtacgtcacc ttgccctttt tcacgatggc aatgccggta gtgccgccgc cgatatccac
     2101 tacaccggcg ttgtccagtt gcagcagctc cgcaactgcg gttggctcat ccagcacatg
     2161 gctcacttcc agtccagcag actccagcac gttgatggaa atacgcgggt ccgtacctgg
     2221 aggaaatgaa gtcgccgcat ggctaaaacg gcgaccgaat tgctgctcga gcgtgtcgag
     2281 atggcgacga acaatggtga cagcgccgaa gaaatcccag acgatgccgt cgcggaccac
     2341 atcggcccag tcgaggcata ccgccaccgg ctgaccgtcg cggtcgacaa ccatcgacac
     2401 cacatcgcag gtgcccagat ccacccccag ccacagcgga gattccgtcg cggcgggcgt
     2461 ctggttacac agcgttgccg ccgtttgcag acgtggggtg agccattgtt cgtcgtgcgc
     2521 catctgttac tccttaaaca atgcgaaacg catcgactaa tacacagcga cgcagacgga
     2581 caaacgtacg cgcgctggtt accccttcac cggttggcgt ggtgatggtc atggtggtcc
     2641 agccttcccc gcccagcccc agcccggcaa tgcacggtcc gttcttaacg aaaatgctgg
     2701 tatcaatagc attcgccatc tggttcatgt tttcgatgtt gcgcgagtgc attgccgccg
     2761 tgtggtggca accgccttcc agtttcaccg ccagcgcaat ggcatccgcc acgttggcga
     2821 cgcgcacgac gggcagcacc ggcatcatca gttcggtcac ggcaaacgga tgttctgcag
     2881 tggtttccac aaacagcaaa cgcgtttctt gcggcacgtt caggccgatt gccgccgcga
     2941 ttttcgccgc atcgcgacca acccagtcac ggctgacggt gccttttccg cgctcgtcga
     3001 tatttttcag caacaccggt tgcagctgct gcgcctgttc tgcggtcagt ttcaccgcgt
     3061 gctggccttc catcagacgc atcagttcat cggctacgct atcaacaaca atcagtacct
     3121 tttcgtcggc acaaatgatg ttgttatcga aagaagcgcc tttgacgatg gactgagcgg
     3181 cacgggcgag gtcggcggtt tcatccacca ctaccggcgg gttgccagcg cctgcggcaa
     3241 tcagacgttt attggtgtgt ttacgcgccg cttctactac cgcttcgccg ccggtcacca
     3301 ccagaaggcc gatgcccgga aacttgaaca aacgctgcgc ggtttcgata tccggatttg
     3361 ccacagtaac cagtaagttt tccggcccgc ctgcggcaac aatcgcctgg ttgagcagcg
     3421 tgatcgcccg ctgggagact tttttcgccg ccggatgcgg ggcaaaaatg acgctgttgc
     3481 ccgcggcaat caggctgatg gcgttattaa ttacggttgc cgccgggtta gtggacggag
     3541 ttaccgaagc caccacgccc cagggcgcgt tttcaattaa agtcaggccg ttgtcgccgg
     3601 tcagcacctg cggagagaga cactcaacgc ctggcgtgcc gcgcgcctga gcgacgtttt
     3661 tggcaaattt atcttcaacg cggcccatgc cggtttcact gacggcaagt tccgctaaat
     3721 ctctggcgtg tttttcgcct gcctcacgaa tggcagcaat ggctaactgg cgcattgcca
     3781 cgctttttaa cccttgctgg gcgactttgg ctgccgcaac ggcgtcatcc agggacgcga
     3841 aaacgcccat ctcatgaacg gcggcgggcg gcgtgtcact gctttgcatt ttcagcagta
     3901 ccgctttcac cacctgttca atatcctgtt gattcatgat gttctgcctt atttgtggaa
     3961 aattacctga ccgccagaca ccacctcatc gacaatgcca atcacgcaca gatcgaccgg
     4021 tgacgtttcg cttttatgcg cctggcgggc ggaactgcca ctcaccagta acacccactc
     4081 cccggttccc gcgccaatat tgtcgatggc gacggcgcat tgcccgtcag gattaccttg
     4141 tggatcaatc atttccacca tcagcaattt gtcatgcgcc agtccgtgat ggcgtacggt
     4201 acaaacaatt tgtccagtga cgactgccag tttcataccc gcctccgtgg cgtatttcag
     4261 gtaaaagctc cccctaccct ccgcagaagg taaaatgaaa aaggagagag cgtaacgccc
     4321 gaatcgacgt cacacagggt gattacaggt tgctgctatc gcctttcagg ccgatcggga
     4381 agacttcttc caggtcgccg tgcggacgcg ggataacgtg tacagatacc agctcgccga
     4441 tacgctgtgc cgctgcggca cctgcatcgg tcgcggcttt acaggctgca acatcaccac
     4501 gaaccatggc agtacacagg ccgccgccaa tctgcttaac accaaccagc ttgacgcgcg
     4561 cagctttcac catcgcgtca gaagcctcaa tcagtgcaac caggccccgg gtttcgatca
     4621 ttcctaatgc ttccattgtg ctttcctctt tatcagggtc cagaacggga ccgttcattc
     4681 aaccagtgtt tgtaaactgc tttcgcggtt cacttctgtc tgacgcggca cagctgccac
     4741 cagcgccagc tcgataattt cctgcacgct acaaccacga gagagatcgt gcatcggcac
     4801 ggcaagtcct tgtatcagtg gcccgacggc acgatatccg ccgagtcgtt gtgcgatgtt
     4861 gtaaccaata tttccggctt ccagcgacgg aaaaaccatc acattggcct tgccctgtag
     4921 cgggctggca ggcgcttttt gcgccgccac ttccggcacg aaggcggcgt caaactgtaa
     4981 ctcgccatcc accaccagct ttggtgcgcg ctcacggacg atttctgtcg cctgctggac
     5041 gttagcaaca caggggtgac gggcgctacc attgctggaa aacgacagca tcgccacgcg
     5101 cggctcttct ccggtgatgg cgcgccaggt ttcggcactg gcaagcgcga tatccaccag
     5161 ctgcgccgcc gtcggctgtg gcaccacgct gcaatcggca aagcccaacg ccgggccgct
     5221 gtactgtggc aacatcagga aaatgaaaga gagcgtttta cagcctggct gcaagccgat
     5281 aatgcgtaat ccggcacgca gcacatttgc cgtggaagag aggttgcctg cgatacagac
     5341 atccgcttta ccggcgctga ccattgctgc ggcgaacatc agagggtcgg taagtttttc
     5401 cagcgcatcc ggcggcgttt tttcgcccgc gcgggccagc cagcgatgag caaattcttc
     5461 ccgcattgcg aggttgccat gcggatcaat cacctgtagc ccgtccatcg ccacgccgtg
     5521 actgagcgca aactgacgaa gttcaaacgg attggcgacc agaatgggcg ttgccagacc
     5581 ttgttgatgt aaatattgcg cggctttcag cacacgttga tctaacgcat ccggaaaaac
     5641 cactctggcg ggcgctcgca acgccagttc acgacaacgt tcaataatca ttgcgttccc
     5701 ccagtcgttg ttggatctgc ttcaccgtta gcggctgctt cgttacgctc aggatcatca
     5761 tcacgtagac cgtgctggaa aggcggttca gcgcctgcaa aatgtccggg cgtaatactt
     5821 caaagctgcg ggtgataaag acctgcgccg ctacggtttc ggtttcccgc actttggtgc
     5881 gtagcagatt gagcaacgca gcatcgcgcc cgtggctggc ttccagcacc agatgatcgt
     5941 ggtcgagata gcgcagcggc tgatgagaaa gccgatgcag atcttcgtca gaaagcccga
     6001 caatcgcctg gcagcccagc ggttccccca gcgcatcggc gcgcataatg ttgccaagac
     6061 gcgaacggat atccgccagc cacggctgcc acggttccgc cagttcaatt tgcagccaca
     6121 ctgccagcgc aatggtgctg tcgagaaccg cgcgaaaacc cagacgcgga tcgcttttgg
     6181 cgaccatttt ttccgccgac aggtgagtca gcgtatccgg cttttttgcc accggctggc
     6241 ggcacagttc gcagcacgcc tgcggatgtt catcgctact ggtcagccca tgaacgggct
     6301 gcggcttctg ctgttcatcg tcaacaaaca ggcggccctg ctcgtcaata aacttgatgc
     6361 gcagatggcg gctttccagc aactcccggg cagagggcgt caggcgactg tccgcaggga
     6421 gatggatctc tgcgccttcg ctgagcgtat ggttcgctct tagccatgct tcggtgatga
     6481 aatctttcat agggattgcc agttagccgg ccaggcgaca tacaggaatt tcacgctgga
     6541 tgtcatacca aattcgatgc tggagccttt cgggataaac atcacgtcgc ctgctttggc
     6601 gatcatggtt tggccttcgt ggcgaacatg cagcacccct tccagcacca tgtcgatttc
     6661 gtcgtagttc agggtccacg ggaagaatgc gttctcccac tgcataaaac cggcggccat
     6721 gctgctgccg tcgtctccgg tcacaagatc agtcaagccc acgcaatgcg gctctgcgcc
     6781 gtcgaaacga ccaaacttga cgctgctgcc atcaatcact ttgatgccgc ctttgccggt
     6841 caccgatttg aagctcggct gcattgcgcc ctgctccagc gactgttttt ccttcatcac
     6901 tttttccatc agctgtgcga ccaggctttc ggtaaactgg ccttccggca gctgggcaat
     6961 gatggtttcg cggatgcgct ggctttcggt tttgtcggct ggcacgctgg cgggcacgga
     7021 cgccgttacc gggatcgatt catcacattc ggtgatggta aaacccagta gatccgccac
     7081 ttcacgggct tccggggtaa taatgctggc gcgcagaacc acggacattg cctgttcgcc
     7141 gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt tttttcacct gcttcctcct
     7201 gtttggtgag agatgccaga taatccacca gttgctgcac gctttgcggg tcgtggctgt
     7261 ttagctcgaa aatcggctct tcgaaacccg tctcaagcaa taactttcgc gttgcggcga
     7321 cgtcagcatc tggcatgtcc gttttactga tgacggcgat ttgccgtttg ctgacgccaa
     7381 tatccagcaa cccggcaggc aaccgacttt ccgggtcatt tgcgccatgg acatagatca
     7441 gcatgtctac atcctgtagc gtggtaatta aggcgtgata ccagcgggga tggctaaaat
     7501 attcacccgg cgtatcaata tcgcccttat cattaaattc cacggcctgt gtttttctgg
     7561 cgagggtata attcccctgc aacgcattaa ataacgttgt ttttcctgcc ccgacactac
     7621 ccacaaacgc aattcgtttc atgaatgcct cgtattaact tttggtcatt tcacaaagcg
     7681 tataatttaa taagcgacct aaaccgctga cggtttgtga taacgcttct tctactgcgc
     7741 ccaccgagcc ataaatcacc aatgcaccgc tgaagcggtc gagaaaaccg atatgcacat
     7801 cagcggcttt cagcgctaaa tcaccggcga tcatcgccgt ttcgccggga gttagcgtca
     7861 taatgccaat tgcgcccgca tccggaacgc cgattttctt cgccagttcc tcaccgggat
     7921 gcgagatgag atgcaccagt gtgacctgtt tgccgggcac aaattcctga atgatgcgtt
     7981 ctttatccat gagtcgccgc ctgtgaaaaa ctttcgtgta tcgtgacaat ttttcgtcag
     8041 agtaaataac aaaattcggc aagcgcgttt aaaaggtgag gtagatcact aagaatggag
     8101 agatattcgc tgtggtgcat aaactttcat atgcaacgca tgaatataaa taaaataata
     8161 tcgagatatt atatagactg cttttttatt attcgcaaac tcagtaataa gctttactaa
     8221 tatattgcag ccaaaccgct taccctgaat attcagggta agcgtgagag ttaaaaaaga
     8281 attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatgttg acgatacgac
     8341 gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc agcaccggac
     8401 cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt
     8461 ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc ggcatacggt
     8521 cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagat
     8581 ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt
     8641 cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata ccaaaacgac
     8701 ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct ggttcgtcat
     8761 tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgcg ttcattgcac
     8821 ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacgcta aaatgttcat
     8881 gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga accatgatcg
     8941 cgccgatcac tgtcgggtta ctgatcagcg cccgctgcgc ctgttcctga gtgacaccgc
     9001 gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt
     9061 tattgacgat ctcaaaatca acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca
     9121 tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc gttaccagtt
     9181 cctgagtggc atgcagaacg cgcgcctctt ccccttctgg cagaacaacg cgcttcggcg
     9241 ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa acgaactcag
     9301 tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg cccgactcca
     9361 tcgcggcttt agcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg
     9421 gaatgatgta ttccggacca aagctcagat cctgatcgcc atacgctgaa gccaccactt
     9481 cgctctgttc cgcatgggcg agttctgcaa tcgcacgtac cgccgccagt ttcatctctt
     9541 cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg aagcacagga
     9601 cgttgttcac ctggttcgga tagtcagaac gaccggtgca aatgatggca tccggacgca
     9661 cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg
     9721 gcgcacgagc cattttcttc accatttcct gggtcagcac tttcgggccg gaacatccga
     9781 ggaaaatatc cgcgccttca atcacatcat cgagggtacg tttgccgtcg tccaccaccg
     9841 catatgcggc tttggtttcc gccatgtttg gctcacggcc ctgatagata acgccttttg
     9901 aatcgcaaac cacgatgtta tgtttttgca gacccagcgc taccagcagg ttcatacagg
     9961 cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca
    10021 ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg tgctgatcgt
    10081 cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg
    10141 gcgctttaat gtcttcgagg ttgattccgc cgaaggtcgg ttcgagcgca gcgacaactt
    10201 caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca atcccggcga
    10261 atttcttaaa cagaacgccc ttgccttcca tcaccggttt gccagccagc gcgccaatgt
    10321 tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc caccaggtta ccgcgcgcgg
    10381 tatatttgta ggcttttaac gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg
    10441 gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga gaaacctgga
    10501 ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac tggtcatcca
    10561 tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca gcccctgccc
    10621 acaacgtggc gcacattatt accccgccgg agtctacaga ctttgagcaa gtccaaactc
    10681 tcaccattaa tataatgttt tggtaataat cctataacac tgatgttacc tgcttaatcc
    10741 agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt
    10801 gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta tgaacgagtt
    10861 agacggcatc aaacagttca ccactgtcgt ggcagacagc ggcgatattg agtccattcg
    10921 ccattatcat ccccaggatg ccaccaccaa tccttcgctg ttgctcaagg ctgccggatt
    10981 atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac
    11041 ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat
    11101 tctcaaaatc gtacccggtc gcgtgtcaac agaagttgat gcacgcctct cttttgataa
    11161 agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac aaggcgttga
    11221 gaaatcacgc attctgatca aactggcttc gacctgggaa ggaattagcg cggcagaaga
    11281 gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttcttttg cccaggcgcg
    11341 ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtcgggcgta tttatgactg
    11401 gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg gcgttaaatc
    11461 ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg tgatgggcgc
    11521 gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc
    11581 accgaatttg ctgaaggagc tgcaggaaaa agtttcgcca gtggtacgta aattaatccc
    11641 accttctcag acgttcccac gcccagctcc catgagcgaa gcggagttcc gttgggagca
    11701 caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt tcgccgttga
    11761 tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg gagtgttata
    11821 tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgcg gtacaaaaag
    11881 ccaactctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa gtgctgtgga
    11941 acgattttct taaacataac cctaccgacc caaccgggta tgatcgcgac cgctttattc
    12001 tttccaacgg tcacgcgtcg atgctgctct acagtttgct gcatctgacc ggttacgacc
    12061 tgccgctgga agagctgaag aacttccgtc agttgcattc gaaaacccca ggccacccgg
    12121 agattggtta tacgcccggt gttgaaacca ccaccggtcc acttggacaa ggtttggcga
    12181 acgccgtcgg actggcgata gcagagcgta cactggcggc gcagtttaat cagccggatc
    12241 atgaaattgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg atggaaggta
    12301 tttcccacga agtctgttcg ctggcgggca cgctaggact gggcaagctg attgtttttt
    12361 acgatcacaa cggtatttcg attgatggag aaaccgaagg ctggtttacc gacgatacgg
    12421 caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt cacgatccgc
    12481 aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg tcgctgatta
    12541 tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg
    12601 gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc tggcaccatc
    12661 cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa
    12721 aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat ccgcaactgg
    12781 cagaagagtt tacccaacgg atgagcggtg gtttaccgaa ggactgggag aaaacgactc
    12841 agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc
    12901 aaaatacg
//
LOCUS       DADLTD010000122        12637 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLTD010000122 DADLTD010000000
VERSION     DADLTD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.122"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(4..324)
                     /gene="chbB"
                     /locus_tag="JN701_002692"
     CDS             complement(4..324)
                     /gene="chbB"
                     /locus_tag="JN701_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY7365097.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(622..960)
                     /gene="osmE"
                     /locus_tag="JN701_002693"
     CDS             complement(622..960)
                     /gene="osmE"
                     /locus_tag="JN701_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY7365098.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1162..1989
                     /gene="nadE"
                     /locus_tag="JN701_002694"
     CDS             1162..1989
                     /gene="nadE"
                     /locus_tag="JN701_002694"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY7365099.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2219..3106
                     /gene="cho"
                     /locus_tag="JN701_002695"
     CDS             2219..3106
                     /gene="cho"
                     /locus_tag="JN701_002695"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY7365100.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3066..3641)
                     /gene="ves"
                     /locus_tag="JN701_002696"
     CDS             complement(3066..3641)
                     /gene="ves"
                     /locus_tag="JN701_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY7365101.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3844..4315)
                     /gene="spy"
                     /locus_tag="JN701_002697"
                     /pseudo
     CDS             complement(3844..4315)
                     /gene="spy"
                     /locus_tag="JN701_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
     gene            complement(4645..5613)
                     /gene="astE"
                     /locus_tag="JN701_002698"
     CDS             complement(4645..5613)
                     /gene="astE"
                     /locus_tag="JN701_002698"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY7365102.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5606..6937)
                     /gene="astB"
                     /locus_tag="JN701_002699"
     CDS             complement(5606..6937)
                     /gene="astB"
                     /locus_tag="JN701_002699"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY7365103.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDCSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVKMNDTLFNALND
                     WVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6934..8411)
                     /gene="astD"
                     /locus_tag="JN701_002700"
                     /pseudo
     CDS             complement(6934..8411)
                     /gene="astD"
                     /locus_tag="JN701_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(8408..9442)
                     /gene="astA"
                     /locus_tag="JN701_002701"
     CDS             complement(8408..9442)
                     /gene="astA"
                     /locus_tag="JN701_002701"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY7365104.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9439..10659)
                     /gene="astC"
                     /locus_tag="JN701_002702"
     CDS             complement(9439..10659)
                     /gene="astC"
                     /locus_tag="JN701_002702"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HAY7365105.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11096..11902
                     /gene="xthA"
                     /locus_tag="JN701_002703"
     CDS             11096..11902
                     /gene="xthA"
                     /locus_tag="JN701_002703"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY7365106.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12069..>12637
                     /locus_tag="JN701_002704"
     CDS             12069..>12637
                     /locus_tag="JN701_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7365107.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITL"
BASE COUNT         3067 a         3420 c         3095 g         3055 t
ORIGIN      
        1 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
       61 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
      121 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
      181 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
      241 ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa
      301 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
      361 cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
      421 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
      481 taaattgtct ctccttgacc tgcggttatg accctcttca catttcgggc aaataattcg
      541 cgaagataat taagaactca ggccaaaaaa accggcgcaa tggccggttt ccgttgttac
      601 tcaagctttc aaacgaagtg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
      661 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
      721 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat
      781 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
      841 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca
      901 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
      961 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     1021 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttatccacca
     1081 ttcaacccgc tgtcgcttgt cgtttcagta gcgacgggtt agctttaagg aagttttgtc
     1141 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     1201 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     1261 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     1321 acgcttgccg gtaagctgtg ccagatggcg attaatgagc tgcgccagga gaccggcaac
     1381 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat
     1441 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     1501 gcggtattgg ccagcgaaca ggcgctgcgc gaagcgggca ttgaactgag cgattttgtc
     1561 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     1621 agcggtgtcg tggtgggcac cgatcatgca gcagaagcta ttaccggatt cttcactaaa
     1681 tatggtgacg gcggtacgga cattaatccg ctgtatcgtc tcaacaaacg tcagggtaaa
     1741 cagttactgg cggcattagc ttgcccggaa cacctttata agaaagcgcc aacggccgat
     1801 ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     1861 atcgacgact atctggaagg gaaaaacgta cctcaacagg tcgccagaac aatagagaac
     1921 tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg
     1981 aaaaagtaat aatttgcaca tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     2041 tgcggcgtga acgccttatt cgacctataa aactatgcaa attcaataca ttgcaggagt
     2101 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc
     2161 ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     2221 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     2281 cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     2341 tggtgaaagt gacaccatgc cgctctatat cggcaaaagc gttaacatcc gcagccgcgt
     2401 cctttctcat ttacgtaccc cggatgaagc cgccatgcta cggcaatccc gacggatcag
     2461 ctggatatgt accgccggtg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     2521 acaacagccg ctgtttaata aacggttgcg ccgcaatcgt cagctctgtg ccctgcaatt
     2581 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     2641 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga ccatcgctga
     2701 tgaacaaaaa ctttgttatg gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg
     2761 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     2821 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca
     2881 aggggcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     2941 ctggctgtgg ctgggggcgg ttaattcgct ggaagacgcg acaacgttaa ttcggacacc
     3001 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     3061 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     3121 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc
     3181 tggtcggtag tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc
     3241 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     3301 acatccaggc gtgtcatgat gttgaaatcc atcgacatct gcccttccgt cagtttcgct
     3361 tttaccacct ggtccgctgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     3421 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     3481 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     3541 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc
     3601 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     3661 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     3721 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     3781 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     3841 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagaacgct
     3901 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     3961 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     4021 gcgcttcagc ttttgcttta tcgaaggtat cgctgacaat gatgtcatgc attgcgcggc
     4081 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgcggatct
     4141 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     4201 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggca tgggccaggt
     4261 tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta cgcatattct
     4321 atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt gttgacaaga
     4381 ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa
     4441 aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag tgatgagaga
     4501 atattacaac acaatgattt tgcagagatt atgaagaact atactggatg actggtgata
     4561 aataaagcaa ataaccagga ttaatctgta ttaatttata agaaagcaac ttaatacccg
     4621 cagaatgatt tctgcgggta agtattagct tattttttcg agcattaatc ccgcgcgtaa
     4681 tcccaacgct accaacggat tagggaataa cacatactct acatcatggg ttacggtaaa
     4741 acgttcctct ccgtcctgcg ccagcaatgt tcctttctca aacggcataa aattcagcgt
     4801 gtcacttgcc atatgcattt cgaaggacgg cgagtggcga gtaatttgcg aaaccacccg
     4861 ataacggagc ggcggtgttc tcacgatacc gacactctca ccagatagca gcgcagcaat
     4921 tgcgctggca gttactgcaa actggcgaag atcgttttgc ccaaagggca acgctttgcc
     4981 aagttccagc gtacaggcca gcgcgccaaa atgtctggcg ctgaaatggg taaacgtacc
     5041 accaggttcc tgatggaaca ccagcgcctc cagccccgcc gcacccagcc acgtcagaaa
     5101 tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg aactgcggat gcaaggagcc
     5161 acgaattgcg gtatgtagat caaggtgcca gcgcacagat tctttgccct ggtcataaaa
     5221 atcttccagg cactgttcca gttcgcgcgc ccgacaggtt tctccgcttt cagcaaatag
     5281 ctgccaacga ccgccaaaca ttcgattcat atcgctatgg caataacgtt tcccttgctt
     5341 cagcgcagga ggattcccga ggatcaccag caaccgccaa cgtaacggga tctcgccgtg
     5401 agatatcgcg ccaagcaacg cgtccagcat ttccacaggt gccgtctcat taccgtggat
     5461 ccccgcagaa atcaccagcg cgccttgcgg tggcgttaat ggcgtcagtt ccagcacacc
     5521 atcgcccagc cagcgccagc gaacgccgtt gatttctcgc tcggtgataa ccggtttttt
     5581 acccgttaag gtcagagcaa gaaaattatc cattgccccc tccctcgcgc tggaacggat
     5641 aaaccgaacc gagattcagt aattggctca atacatccag tgcttcccgc ccttcgcgca
     5701 gcaattgcgg gtcggccaga tcggcagcag taagacgatc gcggtagtaa cgatccaccc
     5761 agtcattgag cgcattaaac aaggtatcgt tcatcttcac cgcccggcgt tcttcttctg
     5821 tcaataccac ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta
     5881 agtcaaagac ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac
     5941 cccatactcc ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaacaatcat
     6001 cgcggctcaa cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct
     6061 gagttgccgg aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta
     6121 actgtgactg gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg
     6181 caatcacgtc attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa
     6241 tcacctgttg gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct
     6301 gtcgcgccgg ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa
     6361 gttgcatacc cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat
     6421 caccgagcaa cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa
     6481 aaatagcttt taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt
     6541 tcaggttggc aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg
     6601 ttgccgcatt ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg
     6661 cctggcgtgc aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca
     6721 gcaccggaat aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cctgcatcgg
     6781 caagggtttt cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa
     6841 aacggtgacg ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc
     6901 ctaccagccc gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc
     6961 cagcccgggg ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg
     7021 ccatgcgcag taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat
     7081 gccgccgaat ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc
     7141 cgcccgcgcc tccagcaaca gttgatcgaa tttttcccgt tcgggggaaa ccagaccgca
     7201 agagagtccg aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg
     7261 ccagacgcgc aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat
     7321 ttcaatgatc cccggcgtca gcaacgatgt ctctaattgt aataagcgcg gcgcaagcag
     7381 ggttcgtccg cccatcgctt cagttgctgc caggcagtaa ccacctgctg tgcggcctgt
     7441 tcagaaatca gcccgccaat aaacggctgc ggttcgtcat cccagttgcc cggcgttaat
     7501 cgctggctga cggcaaccag acgagcaaga aacgcatcgc cctgcgctcc gcttttgagc
     7561 aataaacggc gggcgcaggt gcagcgttgc ccggctgtga caaacgccga ctgaatggtc
     7621 agatggacag ccgcgtcgat atccgccacc tcatcgataa ttagcggatt attaccgccc
     7681 atctcaaggg cgagaatttt ctccggctga ccggagagct ggcgatgcag ctggtagccg
     7741 gtattggcgc gaccggtaaa cagcaaaccg tcgagatcct ccagcgcact cagcgcctga
     7801 cccgtttcac gcccgccctg caccaggttc agcacgcccg gcggcaagcc agcctgctgc
     7861 cataaacgca ttaccgcttc gccactccac ggtgtcagtt cgctgggttt aaagataatg
     7921 gtgttacctg ccagcaatgc cggaacgata tgtccgttcg gcaaatgacc agggaaatta
     7981 tacggcccaa acaccgccag cacgccgtgc gggcgatgtc gcaggctcgc cgcaccgtcc
     8041 ggcatttcac tacgctgctc gccggtacga acgtgatacg ccttaattga tatcgcgatt
     8101 ttattgatca tcgccgtcac ttcggttgcc gcttcccagc gcagcttacc cgtttctctg
     8161 gcaataatcg cggttaattc ggctttattg ctttccagca atccggcaaa gcgttcgaca
     8221 acgacctgac gttcagccaa tgagagccgc gcccagcgcg gaaacgccgc acgggctgcc
     8281 cgacaagcct gttcgacctg agcggcatcg gcatcattgc cttgccataa cacctcgccc
     8341 gataccggat tacgcttcac acgcgatgcg ccctggcccg ttacccagtc accgttaatc
     8401 cataaagtca tgctgttttc tccactgcgc acaggcgcac cagacgaacg cgatcccctg
     8461 cgtggcattt gagggcatcc agttgtgcgg cggttaaaat caaacgctcg gttgccggat
     8521 cggtacgcgc cagcaccacg cggaaatggt gataattttc attggcgacc aggcaggccg
     8581 gaaaatcgcc ctgcgcaggc tgcccttctg ccacttccac cagccgactt ttacggatgg
     8641 cgcgcacgcg gtcgatgtca cactcaagcg ttggcccgcc gtcgaagatg tcgatatagt
     8701 tgcggtaacg aaaaccttct ttctccagca ccgcgcgggc aggcgcggtt tgcggatgta
     8761 cctgaccgat gacgtcctgg gcttcctggg ataaaaagtg ggtatagatc ggatgtttcg
     8821 gcatcagttc tgcaataaat gccttttgcc cggtgccgca gagaaaatcg gcgcggctaa
     8881 aatccatcga aaagaagcgt ttaccgaggc tttgccagaa cggtgaatag ccgtgttcgt
     8941 caatcacccc gcgcatttca gcaaccactt tgtcattaaa cttgtcgcga aaagccgcca
     9001 taaacataaa gcgcgatttc gacagcaaat agccgttgcc ctctttgcgc cagtccgggt
     9061 cgagaaacag cgtgcacagc tcgctgctgc cggtgtgatc gttactaaga aacagcgtcg
     9121 gcaatgcgtt atagacattc agctcttttg aggcgtgaac caacgtgccg acccgatagt
     9181 tgtgccaggg atcgttcagc ccaaccgcca cctcaatggc gcaaatcccc gccacggtgc
     9241 ctgtctcgct atcttccagc acgaacacat agccctgctc gcttttgggc agttcgcctt
     9301 gccaggtttt gattgccctt tcgatacgcg cggaaagcgt ggcttcatta gcgggaagcg
     9361 acgtcaggcc gccgcccgtt ttgctggcaa gctgcatcag cgccgagaca tctgagcgct
     9421 caacgggacg gatgaccatc atgatgaacc tcggctaaca aaatgttcgc aagcagctgc
     9481 aaagcgatcc agtccggtcg tcacctcttc ttcgctgaca ttgagcgcag gcgcaaaacg
     9541 caccacgttg ccacccgcaa tcagtaccat aacgcctgct ttcgccgcct cctgagagat
     9601 ctgtttcgct tgtccggcgt aatcggcatt cagtacacag ccaatcaaca aacctaagcc
     9661 gcgaacttca ctgaacaaac cgtagcggtg attaatggta ttaagacgct caacaaacca
     9721 gtcgtgacgc tgtttaacgc cattaagcat ctctggtgtg ttgatgagct ccagcacttt
     9781 gcccgccacc gccgaggcca gcggattgcc gccataggtg gtgccatgag tgccaacggt
     9841 catcacgctg gcgcactctt cggttgttaa caacgcaccg accgggaaat cgccgcccag
     9901 cgctttggcg gtagttaaca gatcaggtgt cacgccgtag tgcatatagg catacagttc
     9961 cccggtgcgc ccgacgccgg tttgtacttc atcaaaaatc aacagcgcat tgtggcggtc
    10021 acacaattca cgcagacctt gtaaaaacgc gttgctggct ggcaccacac cgccttcccc
    10081 ctggatgggt tcgacaatca ccgcacaggt ggcgtcgtca atcagcgcgc tggcagagtt
    10141 aatatcgtta tatgcagcat gacggatatc tggtggcagt ggcgcaaaat cctgtgaata
    10201 ggctggctgc ccacccgcac tgacagtaaa cagcgtgcga ccatgaaacg catttttaaa
    10261 cgccatgata ccgctcttat ggctgccgta gcggtcgtga gcgaatttac gcgccagttt
    10321 tagcgccgct tcgttggctt ccgcaccgga gttacaaaag aagacgcgat cggcaaacgt
    10381 ggcgtcgatc aattttttcg ccagtcgcag taccggctcg ttggtgtaac cgttgccggt
    10441 atgccagaac ttactcgcct gttcgttcag cgcttcacgc agttccggat gcgcatggcc
    10501 cagcgcgttc accgcaatgc cacccgcgaa gtcgatatac tctttcccct gctgatccca
    10561 caagcgcgaa ccttcgccac gtaccggtat aaagggtgcc ggagcgtaaa caggtatcat
    10621 ccattcatca aagttttcac gcgtaattgg ctgagacata gcgacctcta cagtaaataa
    10681 ttcgttattt atatgttaac aataagtaat gtttgcgctg taaatgtaga ttgcagggtt
    10741 cgtgccagcc agtgataaaa gtgcataaac ggcggaggct aactggaaat caaggagtta
    10801 taaccaaacc atatgcattt aaagtgcata taaagtgaat acgtttgcga tgtgggtgaa
    10861 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
    10921 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
    10981 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
    11041 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
    11101 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
    11161 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
    11221 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
    11281 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
    11341 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
    11401 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
    11461 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
    11521 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
    11581 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
    11641 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
    11701 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
    11761 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
    11821 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
    11881 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
    11941 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
    12001 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
    12061 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
    12121 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
    12181 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
    12241 ccaccctctt tctgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
    12301 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
    12361 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
    12421 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
    12481 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
    12541 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
    12601 tgatggcaag cgatctcgcc aatgaaggca ttacgct
//
LOCUS       DADLTD010000123        12630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLTD010000123 DADLTD010000000
VERSION     DADLTD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.123"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="JN701_002705"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="JN701_002705"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY7365108.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1380..2186
                     /gene="tcdA"
                     /locus_tag="JN701_002706"
     CDS             1380..2186
                     /gene="tcdA"
                     /locus_tag="JN701_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY7365109.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="JN701_002707"
     CDS             complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="JN701_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY7365110.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="JN701_002708"
     CDS             complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="JN701_002708"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY7365111.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4077..4304
                     /locus_tag="JN701_002709"
     CDS             4077..4304
                     /locus_tag="JN701_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY7365112.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4655..5572
                     /locus_tag="JN701_002710"
     CDS             4655..5572
                     /locus_tag="JN701_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY7365113.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5591..5986
                     /locus_tag="JN701_002711"
     CDS             5591..5986
                     /locus_tag="JN701_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY7365114.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5979..7079
                     /gene="rlmM"
                     /locus_tag="JN701_002712"
     CDS             5979..7079
                     /gene="rlmM"
                     /locus_tag="JN701_002712"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY7365115.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7123..7854)
                     /gene="fucR"
                     /locus_tag="JN701_002713"
     CDS             complement(7123..7854)
                     /gene="fucR"
                     /locus_tag="JN701_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY7365116.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7912..8334)
                     /gene="fucU"
                     /locus_tag="JN701_002714"
     CDS             complement(7912..8334)
                     /gene="fucU"
                     /locus_tag="JN701_002714"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY7365117.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8336..9755)
                     /gene="fucK"
                     /locus_tag="JN701_002715"
                     /pseudo
     CDS             complement(8336..9755)
                     /gene="fucK"
                     /locus_tag="JN701_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(9752..10951)
                     /locus_tag="JN701_002716"
                     /pseudo
     CDS             complement(9752..10951)
                     /locus_tag="JN701_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            11067..11714
                     /gene="fucA"
                     /locus_tag="JN701_002717"
     CDS             11067..11714
                     /gene="fucA"
                     /locus_tag="JN701_002717"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY7365118.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            11742..>12630
                     /gene="fucO"
                     /locus_tag="JN701_002718"
     CDS             11742..>12630
                     /gene="fucO"
                     /locus_tag="JN701_002718"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY7365119.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTG"
BASE COUNT         3002 a         3111 c         3386 g         3131 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaatca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcgg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ctggatgtcg gtggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac atcgcgcagg ttggtacaac cactatggac gtgtctgggt
     1261 gctgaaaacc gccccgggcg caggtaacgt ctttagcggc tgatgtgtta ttctgtatac
     1321 cgtttttacg gatacgacag ggtgagggat aacctcaccc tttttaattc tgaggtgcta
     1381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
     1441 gtgaaaaagc gttgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
     1501 tcggttcctg ggcggcggaa gcgctggcgc gcacggggat tggcgcaatc acgcttatcg
     1561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
     1621 tcgggctggc aaaagcggaa gtcatggcgg agcgcattcg ccagattaac ccggagtgcc
     1681 gtgtaacggt ggtggatgat ttcgtgacgc cggataacgt agcgcaatat atgagtgccg
     1741 gttattcgta cgtgattgat gccattgata gtgtacgacc caaagcggcg ctgattgctt
     1801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgcgggtggg cagattgacc
     1861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggacccgctg gcggcgaagt
     1921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtataggt aagctcggcg
     1981 tggattgcgt gttttctact gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg
     2041 cgatgaaagc cacggcagaa gggccgaagc ggatggattg tgcatctgga tttggcgcgg
     2101 caacgatggt gaccgccacc tttgggtttg ttgcggtttc tcatgcgctg aagaagatga
     2161 tggcgaaagc agcgcgtcag ggttaaacaa tcaggtcgga tgcgacgcga acgtcttatc
     2221 cgacctggtt ctgtgcttaa acctgcttcg ccgcagcgat aattgcctcg cttaacgcat
     2281 tcaacccctg gctgcgtgag gcgctaagct gcccacgcaa tcccagctca tcaaacaatg
     2341 ccagcggcga ctgtgcctgt aattcggcgg cggttttccc ctcaacggca gtcaacaaca
     2401 ccgccaacag gccgagcaca atgcgccctt cgctgtcgcc aaagaagtgc attttaccgt
     2461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
     2521 gcgcttttaa ctcgtctggc aaagccggaa gctgtttccc cagcatgatc aactggcgat
     2581 atttatcttc ccattgcgtc aacggtgcga aggtattgcg taacgtttct gcggttacgg
     2641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
     2701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
     2761 agaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cacaatgttg
     2821 cccggcccgc aaggcaatac cgtactccgc cagtagcgtc accatatcgc tgtgatgaac
     2881 gccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
     2941 acgtttcgcc agcgcatctt ccgccagcgt tgctaagcta tggctccagt tttcggcctg
     3001 gttgatatcg taatctgcca gccattccag cgccgcgctt aatcctatga caccagccac
     3061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtcgtgaagc cgtcaaaact
     3121 cacttcgtga atcattttgc cgccacccag ccagggcgac atcgcttcca gcagttctga
     3181 tttgccatac agcacgccga tacccgtcgg gccatacagt ttgtgaccag agaaagcata
     3241 gaaatcgata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
     3301 aaccatcacc accatccctg ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca
     3361 accgccagta acgttcgaca tctgactcaa cgccagaatc cgactacggg gagtaatcag
     3421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
     3481 ggctccagtt tgttgggcga ccatcagcca ggggacgagg ttggcgtggt gttctgccac
     3541 gctgacaata atctcatcgc ccggttgcag acgcggacgc gcatagcatt gtgccaccat
     3601 gttgatggat tcagtggtgc cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag
     3661 taattgcgcc actttctctc gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc
     3721 aaactggctg cgatggacgt ttccggcgct caggctgtaa aactgtcgag tggcttcaac
     3781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
     3841 tgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
     3901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
     3961 tatctggata tcctggcgaa gccgcgtaag taacatatgc tgaatagtgt taggcgttaa
     4021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
     4081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
     4141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
     4201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
     4261 tcaaccgtac tgacgtgaaa gagatggtcg aactggatca gtaattcgcg atcgcaaggt
     4321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
     4381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
     4441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaacacg cattccgata
     4501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
     4561 acggacaatt tataatggct cagattaaaa aaactaatag gttacatagt gtgatctaat
     4621 tgttaaattc atttaacatc aaagtttaat agccatgtct aaacgattac caccgctaaa
     4681 tgccttacga gtttttgatg ccgcagcacg ccatttaagt ttcactcgcg cagcagaaga
     4741 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttttggg
     4801 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
     4861 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
     4921 ccgtagcgcc aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
     4981 ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc gaatccaggc
     5041 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
     5101 gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg
     5161 ttcgccgcta ctgctgactg gcgaaaaacc cttgaagaca ccggaagatc tggctaaaca
     5221 tacgttatta catgatgctt cgcgccgtga ctggcagaca tatacccgac agttggggtt
     5281 aaatcatatc aacgttcagc aagggccaat ttttagccat agcgccatgg tgctgcaagc
     5341 ggctatccac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaatcga
     5401 ggccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaacg ctttttatct
     5461 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
     5521 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
     5581 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
     5641 tgtggctctg ggcgcttttg gcgcgcatgt gttaagtaaa accatggggg ccgttgagat
     5701 gggctggatc cagaccggcc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
     5761 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
     5821 gttaggcacg gtgttgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
     5881 gtgggcgttt gtcactccgg ttggcggcgt gagcttcctc gcgggctggg cgttaatgtt
     5941 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
     6001 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
     6061 aaattttcgg ttttgcccgc gtgaaagaga attcgggtta tgtcatttat gaatgttatc
     6121 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
     6181 gccagtggtt tgtggtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
     6241 ccattgtcgg catgttacag ggcgtagtag agaagggcgg tgaactgcgt gttgaagttg
     6301 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taaatttact gttccgctac
     6361 gcgctgcctt gcgcgatgcc ggggtgctgg cgaactatga aacgccgaag cgtccggttg
     6421 tgcatgtatt ctttattgca ccaggctgct gctataccgg ttactcatac agcaacaata
     6481 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt
     6541 ccacgctcaa actggaagag gcatttcatg tgtttattcc tgcggatgag tgggatgaac
     6601 gcctggcgaa cgggatgtgg gcggtggatt taggcgcttg ccctggcggc tggacctacc
     6661 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
     6721 tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac
     6781 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggctaaa gttgcggcat
     6841 tgatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaat ctcaaactgc
     6901 cgatgaaaaa acgctacgaa gaagtatcac acaatctggc gtatattcag gcacagcttg
     6961 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
     7021 tgacggtgca cgtccgccgc atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
     7081 aggcaaggcc ggggcgtcgt cttcggcctg ctaccctttc cgtcaggctg ttaccaaaga
     7141 agttgcaacc tggcgctcat cagaaataat gtgcgttacc tcatccagat gcccgatgcg
     7201 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
     7261 ttttaatagc atcgatttgt aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct
     7321 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
     7381 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
     7441 cagttgaatg cgttcgcgtt taccgagttc atagcaaatc ggatggctat tggtgaagac
     7501 ctgaatgttg atgtcaggca actggcgtgc cagataccag caagttgaac tggcgtctaa
     7561 ggctatcacc atcccttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
     7621 cgcataatgg cttttcagcc tgatgtgaaa gggatcgcca ctgtcttgat tttgacggtg
     7681 gatatattta gcgcgtccat gattgcgcag aattttaccc tgcgtctgta attcattgag
     7741 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
     7801 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
     7861 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
     7921 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
     7981 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
     8041 cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc
     8101 accttctacc gccgccatca tcaccagcgg cggtgcataa ctgtccagtt caaataacgg
     8161 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
     8221 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gtcccatctc
     8281 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
     8341 cttcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
     8401 gtgcgcgggc ttcttcaggg ctgttaaatt cccctacgcc ataccaaccg aacagcgcag
     8461 ctcctgcgac ggtcgtttcg gcgtcgtcga gaacttttac cggaatatca agcatattgg
     8521 ctttaatctg attccacaat gtgttgcgac ttcctccacc gactaacaat aattcagagg
     8581 cacttaaagt gcccgatttt ttccagcatc tgtagattgc gctgtaattg cgcagttaac
     8641 ccttccagcg ccgcgcgata gaaatgcccc cgcgtggtat taagcgtcac tccttgccag
     8701 ccagcgttct gacacgacaa taaatcacac tgcattttta cgccatccgc gctaggcgcg
     8761 ataagacgag cttcttcaat caacatttgc cagggtgttt cagccgtcca gaacagtttt
     8821 ctcacccatt ccagcacgcc ggatgccagc cattgcatac ctgggttata caaccctgcc
     8881 tggctatcca gttcgcaggt ggaaccggcg tactgactta acagcgaagt atcaacctgg
     8941 gcgctacgga ccattaaaat ttcccatgta ccggaagaga gcacgggttc attttggtcg
     9001 gcaccagcgc caaaaagggc gaactgggta tcgtgacctg cggaaatcac cggtatgcca
     9061 acgggtaagc cgagcattgc tgcggcgctg ttctgtagcg taccaatctg ttcacccgct
     9121 tccaccagac gagggaagag tcggcgtgga ataccagtgg cttgtaaaat ttgcggactg
     9181 aaatcgcgtt gctggatatc cagcatctgg ctggttccgg ccatcgtgat atcagtagtg
     9241 aattcgccgg ttaaacggtg gttaatcagc gacgaaataa agaaccaggc gtgcgtgcgt
     9301 tccagcagtt gtggatgatt ttctttcaac cacaccaact tatataacgt attgaaacta
     9361 aaggctccga cgccagaaat agcctgcaac cgctgtgcgg agatcaactg ttcaatattg
     9421 tccataaccg ctgctgttcg cggacatttc cagctaatga tcggatagag cagattgcct
     9481 tgcttatcta ccagagcgcc atccacacca aaggtggtga cggcgatacc gcggatgtgg
     9541 cattcagtca gttcactatt gatttgccga cagcaatcag caaagcgttg caaaatggcg
     9601 tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc attaggcgtt
     9661 gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac attggtcgcg
     9721 ccacagtcga gtaccaggat aacttcttgt ttcataatcg ctcctgcaat acttcaatat
     9781 ccatgccgtg cgcagcccag gcagaaggac gatacacttt ggtctcttca acgttgtgca
     9841 tacataccgg gatacgcagc atggaggcga gagtgataaa gtcagcgcca acgtggccga
     9901 tggtcagaac cccatggtta gcgccccagt tcgccattac cgagtacaca tccgtaaacg
     9961 gccctttacc ggtgaggcgc ggtgcaaacc aggtggttgg ccaggttgag ttggtgcgtt
    10021 tgttgaggat gtcatgcaca tccttcggca attccacgct ccagccttcc gcgatttgca
    10081 gtaccggtcc caggcctttg atgatgttga cacgagtcat ggtgaacggg acgccgcctt
    10141 cggtaaggaa gcgggaagag taaccgccgc cacggaagta ttcgtggatc gccgggcacc
    10201 attcggtagc ggcgaggcaa gcgtcagcct cttgctgaga gatttcccag tgcggcttca
    10261 tcgtcgggtt accttcgctg tcgcgttgtt tacaggaacc gtccagcgca gcagaacctg
    10321 agttgatcaa atggatgatg ccgtgttctg ccagtccatc gagtttatgc cccgttacac
    10381 gctcaattgc ttctggtgac cagtaggtac gcacatcggc aaatacctga gcggtgccgg
    10441 tgagctggtg acccattagc attgccacgc cgttaagact gtcgttttcg gtcgccacga
    10501 caaagggttc gcgcacgcca ttccagtcaa atgaactgtt gaggatcgct tcggcggtgt
    10561 caccattggg atattgatcg gtccagtgac gttgcccctg gaagcccgca gcgatggcgt
    10621 tgtagccaag tgattcttcc acgcgaccaa tatcggccag tttgctgttg ccttgcatca
    10681 tgtcgcggat acacatcgcc atcagtaaac tttcgcgcag aactgcgcgg ctttgctcgg
    10741 cattgcgttg atactgtttg ttattttcat cttcgccata gcggaagttt ttatcagccc
    10801 aggccagtgc catttccagt tcggcttcgt cgtaaatctt ctgatcgata cggcgacgca
    10861 gttcggtcat atccaccgcc tggactttca ttcccagcca ggattcaaag aagttgtgat
    10921 caacgatgga accggcgata cccatcgaaa cactaaatca gtaagttggc agcattacca
    10981 gaatatggtg ggtaattaaa cggctaattc aatagtgtga aaggaacaac attattgccc
    11041 tgttttgaat cagagagagg tagctaatgg aacgaaataa acttgctcgt cagattattg
    11101 acacttgcct ggaaatgacc cgcctgggac tgaaccaggg gacagcgggg aacgtcagtg
    11161 tacgttatca ggatgggatg ctgattacgc ctacaggcat tccatatgaa aaactgacgg
    11221 agtcgcatat tgcctttatt gatggtaacg gtaaacatga ggaaggaaag ctcccctcaa
    11281 gcgaatggcg tttccatatg gcggcctatc aaagcagacc ggatgccaac gcggttgttc
    11341 acaatcatgc cgttcattgc acagcagttt ccattcttaa ccgaccgatc cccgctattc
    11401 actacatgat tgcggcggca ggtggtaatt ctattccttg cgcgccttat gcgacctttg
    11461 gaacacgcga actttctgaa catgttgcgc tggctctcaa aaatcgtaag gcaactttgt
    11521 tacaacatca tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta tggctggcgc
    11581 atgaagttga agtgctggcg caactttacc tgacgaccct ggcgattacg gacccggtgc
    11641 cagtgctgag cgatgaagag attgccgtag tgctggagaa attcaaaacc tatgggttac
    11701 ggattgaaga gtaatttcgt aaagcaacaa ggagaaggat gatggctaac agaatgattc
    11761 tgaacgaaac ggcatggttt ggtcggggtg ctgttggggc tttaaccgat gaggtgaaac
    11821 gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa tgcggcgtgg
    11881 tggcgaaagt gaccgataag atggatgctg cagggctggc atgggcgatt tacgacggcg
    11941 tagtgcccaa cccaacaatt actgtcgtca aagaagggct cggtgtattc cagaatagcg
    12001 gcgcggatta cctgatcgct attggtggtg gttctccaca ggatacttgt aaagcgattg
    12061 gcattatcag caacaacccg gagtttgccg atgtgcgtag cctggaaggg ctttccccga
    12121 ccaataaacc cagtgtaccg attctggcaa ttcctaccac agcaggtact gcggcagaag
    12181 tgaccattaa ctacgtgatc actgacgaag agaaacggcg caagtttgtt tgcgttgatc
    12241 cgcatgatat cccgcaggtg gcgtttattg acgctgacat gatggatggt atgcctccag
    12301 cgctgaaagc tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg tatattaccc
    12361 gtggcgcgtg ggcgctaacc gatgcactgc acattaaagc gattgaaatc attgctgggg
    12421 cgctgcgagg atcggttgct ggtgataagg atgccggaga agaaatggcg ctcgggcagt
    12481 atgttgcggg tatgggcttc tcgaatgttg ggttagggtt ggtgcatggt atggcgcatc
    12541 cactgggcgc gttttacaac actccacacg gtgttgcgaa cgccatcctg ttaccgcatg
    12601 tcatgcgcta taacgctgac tttaccggtg
//
LOCUS       DADLTD010000124        12424 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLTD010000124 DADLTD010000000
VERSION     DADLTD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.124"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="JN701_002719"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="JN701_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY7365120.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEGFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="JN701_002720"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="JN701_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY7365121.1"
                     /translation="MPSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="JN701_002721"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="JN701_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY7365122.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHAIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="JN701_002722"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="JN701_002722"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY7365123.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="JN701_002723"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="JN701_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY7365124.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="JN701_002724"
     CDS             complement(8214..9107)
                     /locus_tag="JN701_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY7365125.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="JN701_002725"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="JN701_002725"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY7365126.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /locus_tag="JN701_002726"
     CDS             complement(10004..10186)
                     /locus_tag="JN701_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY7365127.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="JN701_002727"
     CDS             complement(10238..11470)
                     /locus_tag="JN701_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY7365128.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..>12424)
                     /gene="lpxK"
                     /locus_tag="JN701_002728"
     CDS             complement(11507..>12424)
                     /gene="lpxK"
                     /locus_tag="JN701_002728"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY7365129.1"
                     /translation="GLVSGAIRLCYKLKLKRAWRAPVPVVVVGNLTAGGNGKTPVVVW
                     LVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTTTAQAGDEPVLIYQRTDAPVAVSP
                     VRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVEIVVIDGVRRFGNGWWLPAGPMRE
                     RAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNLRTGTRCDVAQLEHVVAMAGIGHP
                     PRFFATLKMCGVQPEKCVPLADHQSLNHADVSALVSAGQTLVMTEKDAVKCRAFAEEN
                     WWYLPVDAQLSGDEPAKLLTQLTSLASGN"
BASE COUNT         2862 a         3533 c         3091 g         2938 t
ORIGIN      
        1 tatgtgccat ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactcg cctgagaagg cgcttcgtca gttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacatgaaca tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gctcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag acggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccaaccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccaa
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttga ggcgcacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ctcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcaccagttt
      841 ctgttcacgg gaggttaatt cttcggtcag acggctaagt tcgatctcca tctgttcgat
      901 agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataagccgcc acgaagaact gaattttacg ctccggacgt ttcggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcacgac ggtgtaagcg
     1141 gcgctcaacg ccgttatctt tcaccatgcg catcaccgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 gttgtccatc tccgcttcgc agaaggtaag cgctttttcc agttgattgc ggcgtgaacg
     1321 gttattgctc agttgcgcat gcagctcgtc acggcgaata cgcgcccgct cttctgcccc
     1381 gctatcagca cacacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgaccg cgcaacgctt cgcgagcgcg ggtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagt ttttcgttga gatcgctgtt accgctaagc atttctgccg agtcagagta
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgttgagagt acgcgtaatc ttcttttaac tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctatca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ctctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattacttaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacaca accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 agttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatctcct tcgatcagat agagatcttc
     2341 cgggcaatcg gtcaagccat ctaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacaccacc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacgg cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa cccttcgccg cactgttcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgctcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gtgcttccag ttcctgatgc agggcttcca gttcgtcgat
     2881 atcaaaattc ttgccctgac gtttgcagaa atctgccagc agacgctcag cttcttgctg
     2941 ctcgcgtaga cgctgttcca gttcgcttaa tcgcatccgc agcggctgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcaa tagttcgcga gcgacatccc acgcctcgtt
     3061 acgcgccagt gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcac gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gcagtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa cagatagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtacttttt cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccggcat cacttgttca
     5221 atatttgtcg atggcatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgccagccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctacctgca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccacg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgcca gcatccagca cacgcagttt ttgcgggccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaaggg ttaaatcctc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccatg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatgc caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggtttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagcgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atgggaattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ctaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggcc
//
LOCUS       DADLTD010000125        12198 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLTD010000125 DADLTD010000000
VERSION     DADLTD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.125"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(93..1184)
                     /gene="ychF"
                     /locus_tag="JN701_002729"
     CDS             complement(93..1184)
                     /gene="ychF"
                     /locus_tag="JN701_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY7365130.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1301..1885)
                     /gene="pth"
                     /locus_tag="JN701_002730"
     CDS             complement(1301..1885)
                     /gene="pth"
                     /locus_tag="JN701_002730"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7365131.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2163..2441
                     /gene="ychH"
                     /locus_tag="JN701_002731"
     CDS             2163..2441
                     /gene="ychH"
                     /locus_tag="JN701_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY7365132.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2496..4175)
                     /gene="dauA"
                     /locus_tag="JN701_002732"
     CDS             complement(2496..4175)
                     /gene="dauA"
                     /locus_tag="JN701_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY7365133.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(4300..5247)
                     /locus_tag="JN701_002733"
     CDS             complement(4300..5247)
                     /locus_tag="JN701_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY7365134.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5398..6249)
                     /gene="ispE"
                     /locus_tag="JN701_002734"
     CDS             complement(5398..6249)
                     /gene="ispE"
                     /locus_tag="JN701_002734"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY7365135.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6249..6872)
                     /gene="lolB"
                     /locus_tag="JN701_002735"
     CDS             complement(6249..6872)
                     /gene="lolB"
                     /locus_tag="JN701_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY7365136.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7086..8342
                     /gene="hemA"
                     /locus_tag="JN701_002736"
     CDS             7086..8342
                     /gene="hemA"
                     /locus_tag="JN701_002736"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY7365137.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8384..9466
                     /gene="prfA"
                     /locus_tag="JN701_002737"
     CDS             8384..9466
                     /gene="prfA"
                     /locus_tag="JN701_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY7365138.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9466..10299
                     /gene="prmC"
                     /locus_tag="JN701_002738"
     CDS             9466..10299
                     /gene="prmC"
                     /locus_tag="JN701_002738"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7365139.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10296..10688
                     /locus_tag="JN701_002739"
     CDS             10296..10688
                     /locus_tag="JN701_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY7365140.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10692..11501
                     /locus_tag="JN701_002740"
                     /pseudo
     CDS             10692..11501
                     /locus_tag="JN701_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            11537..>12198
                     /gene="kdsA"
                     /locus_tag="JN701_002741"
     CDS             11537..>12198
                     /gene="kdsA"
                     /locus_tag="JN701_002741"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY7365141.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQV"
BASE COUNT         3043 a         3103 c         3164 g         2888 t
ORIGIN      
        1 ggtgaaatga aaaatccacg caattgcgtg gattttatat acttttgcgc tcttcatgag
       61 atttagcgaa acctcatgag acaataaatt aattagacgt tgaaaaggaa gttcatcaca
      121 tcgccatctt tcacgatgta atctttacct tctgcacgca ttttgcctgc ttctttcgcg
      181 ccttgttcac ctttgtaagt gatgaaatct tcaaacgaga tggtttgtgc acggataaag
      241 cctttttcaa aatcagtatg gattttgccc gctgcctgcg gcgcggttgc tccaaccgga
      301 atggtccatg cacgcacttc tttcacccca gcggtgaagt aagtttgcag gttcagcagc
      361 ttataaccgg cacggatcac acggttcagg cccggctctt ccagcccaag ctcctgcata
      421 aactcgtcac gttcttcgtc gtccagttcg gcaatgtctg cttcaacagc agcacaaacc
      481 ggaaccacaa cagaaccttc tttcgccgcg atttcacgca cctggtcaag atatgggttg
      541 ttttcaaaac cgtcttcgtt gacgttggcg atgtacattg ttggtttcag cgtcaggaag
      601 ctcaggtaac gaatagccgc tttctcttca gcgcttaaat ccagcgcgcg cagcatacct
      661 gcattttcca actggggcag gcatttttcc aggaccgcca gctcagcttt cgcgtcttta
      721 tcgccacctt tggctttctt ctgtacgcga tgaatcgcac gttcgcaggt gtcgaggtct
      781 gccagcgcca gttcggtgtt gataacttca atatcgtcag ccgggttaac tttgccggaa
      841 acgtgaatga tgttgtcatt ttcaaagcag cgaacaacgt gaccgatcgc ttcggtttca
      901 cggatgttgg tcaggaactg gttacccaga ccttcgcctt tcgatgcgcc ttttaccaga
      961 ccggcgatat cgacaaattc catggtcgtg ggaagcgtac gctgcggttt tacgatttca
     1021 gccagttgat ccaggcgagg atcaggcatt ggtacgacgc ctgtgttcgg ctcaatggtg
     1081 cagaatggaa agttggccgc ttcaataccg gctttggtca gcgcgttgaa cagggtagat
     1141 ttcccgacgt tgggcaaacc gacgataccg catttgaatc ccatgattta aatcacctta
     1201 atatcttaat aatcaacctg ttattgctaa cagattgcag aaatggaaat aactttgcct
     1261 attatacacg gcactcggca aaaatgccgc agacaacgac ttattgcgct ttaaaggcgt
     1321 gcaatcggtt cgttgctttg gtcaagccat ctgtaaacca catttcagta caacgcgccg
     1381 cttcgtcaat ggcttcatca attaacttct gttcactaac aggcggtttg cctaacacaa
     1441 aaccgacaac tttattttta tcgcccggat gaccgattcc gatgcgtaaa cggtgaaagt
     1501 tagggttatt acccaattta ctgatgatgt ctttcagtcc attgtgacca ccatggccac
     1561 cgcccaattt aaatttggcg acgccaggag gcagatccag ttcgtcgtgg gccaccagaa
     1621 tttcgtccgg attaatgcgg aaaaaactgg ccatcgccgc aacggctttg ccgctgagat
     1681 tcataaatgt agtcgggact aacaggcgga catcttcgcc tccaagagtg actcgcgaag
     1741 tataaccaaa gaatttagcc tcttcgcgca gcggagcgcg caaacgctct gccagtaagt
     1801 caacgaacca ggcaccagca ttatgtcgcg ttgcggcgta ttcagcaccg gggttcgcca
     1861 ggccgacaat caatttaatc gtcacgtttt tttgtcctga gtgtgtacat aactggcgcg
     1921 tagtttactg gttgcgaccc cgcttgacaa aaaactgcgt atcaaatgca gataacgtaa
     1981 taattgcctg agtgggctat tagaaagtca aggtgttcag gcgtttattt gtaaagtttt
     2041 gttgaaataa gggttgtaat tgtgatcacg cccgcacata acccacaggg tgttgtctat
     2101 actttacaca taaggaagag gggtattccc tgttacaacc cagaaagttc cggaggtgac
     2161 atatgaaacg caaaaacgct tcgttactcg gtaacgtgct catggggttg ggtctggtgg
     2221 taatggtggt cggcgtgggg tattcaatcc tcaaccagtt accacagttt aatatgcccc
     2281 agtatttcgc acatggtgca gtgctaagta ttttcgtcgg tgccattctc tggctggcgg
     2341 gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta ctggtgggtt cgccactatg
     2401 acaaacgttg ccgccgtagc gataatcgcc gtcatagcta acaaataatg cagtttgctg
     2461 accagtcagt ttgcgctgac tggtcaatct cgtacttata aatccgccat cgccgcgcga
     2521 cgattcggga agaacgcgag gcgtcccgag attggttgaa tgcctgcgcg agccatagtg
     2581 cgcagtggct ggaattccac gttgcacacg cgcagttcac atccttcggg cagacgcttc
     2641 acaaaacgct ggaacgcatc aagaccacca gcatcaagta ccggaacggc atcccacttc
     2701 agaatcacaa tccgtttgcc ttcaagacgt gactccaggt ccgtgaacaa gccttcagca
     2761 gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa catcgtctgg aacatctacg
     2821 actaccggtg ccaggcgagt catacgtgcg atacgacgca taaacagcag cgatgccagc
     2881 acgatcccca cgctgatggc aataaccata tcaaacagca cggtcagcga catgcacagc
     2941 agcatgacaa tgatgtcatc tttcggtgca tgacgcagca agtcgaccac tttatgcgct
     3001 tcactcatgt tccacgccac catcaacagc agggctgcca tagcggaaag cggcagccag
     3061 gagagcagcg gtgccagtac cagcagggca agaataacca gaatagagtg gatcaccgct
     3121 gagatagggg aagttgcccc ggcacggacg ttagcggcag aacgcgcgat ggcagctgta
     3181 gcggtaatac caccaaagaa tggagcgata atattcccca gtccctgtcc aaccagttcg
     3241 ctgttcgcct tgtgtttcgt cccggtcata ccatccagta ccacggcgca gagcagagat
     3301 tcgattgcgc cgagcattgc cattgagaat gccgcaggca gcagtgtgcg aatagaatcc
     3361 caggttagcg tgaattctga attaggcaga tcccacggca gcaccagttg cggcagcagt
     3421 tgcggaatac cgttaccctg agaaccatcg gccaggacgt agtggaattg cgaaccgatg
     3481 gtagcaacat gtccgccgag caggttaaca atccccatca ccgcgcaacc agccagcaag
     3541 gccggaaggt gaccgggtaa acgaatgccc agacgcggcc aaaaaacaag aatacctagc
     3601 gtcacaatgc caatggcagc atcacccaca ttaatggtcg gcagcgccat aaataatgcg
     3661 ccgacttttt gtagataatg ttccgggaca tgggccattt gcagaccgag aaaatcttta
     3721 atctgcatgg taccgatggt gatcccgata cccgaggtga aacctaaggt gacggaaacc
     3781 ggaatatact caatcaggcg accaaagcgt gccagaccca tcagaatcaa aaagatcccc
     3841 gacagcaagg ccgcaaccag cagtcctgcc agtccaaact gttgcgacac gggatagata
     3901 attaccacaa atgccgcagt cggaccggaa acgctaaagc gtgacccacc cgtcagagca
     3961 atgacaatcc ccgcaacagc tgcggtatat aaaccgtact ggggtgccac accactacca
     4021 atagccaacg ccatcgccag cgggatagca ataatcccga cggttatccc ggcaatcagg
     4081 tcacgggtaa accgtgcggc agtatatttt tctttccagc aagcgtcgat cagagcgcgg
     4141 aaaggcatca catgtgagga aaatattttg ttcacaataa tgtttcatcc gtgagcgcat
     4201 catctgtcaa ctaaatggca ggtgaaggag gcataggtca tacaaatgga tattacagac
     4261 aaaaaaaccc gccgcagcgg gtctttgagc cgggttcgat tagtgttcga acatggcaga
     4321 gatcgattct tcgttgctga tacgacgaat cgcttcggcc agcatacctg acagggtcag
     4381 agtacgcacg ttcggcagtg atttgatttc atcgctcagc ggaatggtat cgcagacaac
     4441 gacttcatca attacagagt tacgcaggtt gttcgccgcg ttgccagaga agatcgggtg
     4501 agtcgcgtac gcaaatacac gtttagcacc acgttctttc agagcttcag cagctttaca
     4561 cagcgtaccg ccagtgtcga tcatatcatc gaccagtacg cagtcacgac ctgcaacgtc
     4621 accgatgata tgcatcacct gggaaacgtt cgcacgcgga cgacgtttgt cgatgattgc
     4681 catatcggta tcgttcagca gcttagcgat agcgcgggca cgcacaacgc cgccgatgtc
     4741 cggagaaacc acaattgggt tatccagatt cagctgcagc atgtcttcca gcaggatcgg
     4801 gctaccaaat acgttatcaa ccggaacgtc gaagaaaccc tgaatctgtt cagcgtgcag
     4861 atccactgtc agcacacggt caacaccgac gctggagagg aagtctgcaa ccactttcgc
     4921 agtgattggt acacgagcgg aacggacgcg acggtcctgg cgcgcatagc caaagtaggg
     4981 gataacagcg gtgatacgac ctgcggaagc acgacgcagg gcatcaacca taacgactaa
     5041 ttccatcagg ttgtcgttag taggggcaca agtggactgg atgatgaaaa tatcaccacc
     5101 gcgtacattt tcattaattt gtacgctgac ttcgccatcg ctaaagcgac ctacagcggc
     5161 gtcgccgagt gaagtgtaca ggcggttggc aatacgttgt gctagttccg gggtggcgtt
     5221 accagcaaaa agcttcatat caggcacgag aagaacctca ggcatgcgtc cattggtgga
     5281 aagaatctgc cgaaaactgt gcgggccagg catgatcctt tccaggcagt gtattaaaga
     5341 gcgcgatgca acgtctggaa caaggtgacg ttgtcaccga aactcagctt gcccggctta
     5401 aagcatggct ctgtgcaatg gggaaagatt aacgcctttc gccacaaagc cattgagcca
     5461 ttccggggct tgctctagca cctggcgggc ttcagactct gtatcaaatt cagcaaagac
     5521 acaggcccct gtcccagtca ggcgcgacgg ggcgtattct aacagccagg aaagcaccgc
     5581 atcaacctcg cgaaaacgtt ttcttgcgat aacctcgcaa tcattgctga attcacattt
     5641 tagcaacgtt tctattgacc tttttggcgt attgcgcggg agttcaggat ctttaaaaat
     5701 caccggagtc ggaatactta caccagggtg cgccaccaga taccacttct ctggcggatc
     5761 caccggcgtt agtatttcac caacgccttc ggcaaacgcg gcatgccccc gaacaaagac
     5821 aggaacatct gcgcccagcg tcagccccat ttccgccagc tcatccatgc ttagcccgca
     5881 ttgccagaga tgatttaatg ccaccaggac cgtcgcggca ttggatgaac caccgccgag
     5941 accgccgccc atcggcaaac gcttgtcaat gctgatattc gcaccgcttc ccgtcggaag
     6001 acgcccgctg tctgccgcag ttttcatcaa caatcgcgct gcgcgaacga tcaggttatc
     6061 ttcatgttcc acgccttcaa cgggcgttaa cagacgaata tccccatcgt cacgaagctc
     6121 aatgctgatg gtgtcgccgt aatcaagaaa ctgaaacagc gtttgcagcg tgtggtaacc
     6181 atccgcacgc tgaccggtaa tgtataaaaa cagattaagt tttgccggag agggccactg
     6241 tgtccgcatt atttcactat ccagttatcc atttttaact tgatgcgttg accaccgtcg
     6301 gtgagttcca tattggctgg catcgcagga tgcgttttgg tgtcataacc accataaaca
     6361 accttccagt ttttgccatt ctggctgtag gtaatttcgc tcaggcggta ctggtcgtcc
     6421 agtttgtagt cggttgcatc acccggtaaa cctaaaatcc actggcgcaa gctgttgagc
     6481 ggaattggca ttccggtcaa tttgccaatc atctcttcgg cgtcatcggc ggtataacgc
     6541 tgacctttat tgtcgactaa ctgcacgtta cccggttgag cattcagctc cagttccgtg
     6601 ctgcccaatg ggttagtgag cagcagacgg tagcgatcct ggccggtttg ctgccagaaa
     6661 aagcgggcgt acactttttg ttggtcagaa atataagcga acgcgccgcg agtctgatac
     6721 tgattaagat tgcgcacgtc ttgctgatgc tgacgccatt gtggcgaatc cgggcttttg
     6781 ccaggacctt tgggcgtggt aacggaacag gcagtgagca caagagcagc cagcggtagc
     6841 aggcggataa gacgaaaatc gggcaggggc atagtgatga caagtccttg agatacgttg
     6901 cagttataac ccttaatgct agcgttaccg tccgctatcg tctatgttca agttgtctta
     6961 attgccagaa tctaacggct ttcggcaatt actccaaaag ggggcgctct cttttattga
     7021 tcttacgcat cctgtatgat gcaagcagac taactctatc aacgttggta ttatttcccg
     7081 cagacatgac ccttttagca ctcggtatca accataaaac ggcacctgta tcgctgcgag
     7141 aacgtgtatc gttttcgccg gataagctcg atcaggcgct tgacagcctg cttgcgcagc
     7201 cgatggtgca gggcggcgtg gtgctgtcga cgtgcaaccg cacggaactt tatcttagcg
     7261 ttgaagagca ggataacctg caagaggcgt taatccgctg gctttgcgat tatcacaatc
     7321 ttaatgaaga agatctgcgt aaaagcctct actggcatca ggataacgac gcggttagcc
     7381 atttaatgcg tgttgccagc ggcctggatt cactggttct tggggagccg cagatcctcg
     7441 gtcaggttaa aaaagcgttt gccgattcgc aaaaaggtca tatgaaggcc agcgaactgg
     7501 aacgcatgtt ccagaaatct ttctctgtag cgaaacgcgt tcgcactgaa acagatatcg
     7561 gtgccagtgc tgtgtctgtc gcttttgcgg cttgtacgct ggcgcggcag atctttgaat
     7621 cgctctctac ggtcacagtg ttgctggtag gcgcgggcga aaccatcgag ctggtagcgc
     7681 gtcatctgcg cgaacataaa gtacagaaga tgattatcgc caaccgcact cgcgaacgtg
     7741 cccaaattct ggcagatgaa gttggcgcgg aagtgattgc cctgagtgag atcgacgaac
     7801 gtctgcgcga agccgatatc atcatcagtt ccaccgccag cccgttaccg attatcggga
     7861 aaggcatggt ggagcgcgca ttaaaaagtc gtcgtaacca accaatgctg ttggtggata
     7921 ttgccgttcc gcgcgatgtt gagccggaag ttggcaaact ggcgaatgct tatctttata
     7981 gtgtggacga tctgcaaagc atcatttcgc acaacctggc gcagcgtaaa gccgcagcgg
     8041 ttgaggcgga aactattgtc gctcaggaaa ccagcgaatt tatggcgtgg ctgcgagcac
     8101 aaagcgccag cgaaaccatt cgcgagtatc gcagccaggc agagcacgtt cgcgatgagt
     8161 taaccgccaa agcgttagcg gcccttgagc agggcggcga cgcgcaagcc attatgcagg
     8221 atctggcatg gaaactgact aaccgcttga tccatgcgcc aacgaaatca cttcaacagg
     8281 ccgcccgtga cggggataac gaacgcctga atattctgcg cgacagcctc gggctggagt
     8341 agcagtacat cattttcttt ttttacaggg tgcatttacg cctatgaagc cttctatcgt
     8401 tgccaaactg gaagccctgc atgaacgcca tgaagaagtt caggcgttgc tgggtgacgc
     8461 gcaaactatc gccgaccagg aacgttttcg cgcattatca cgcgaatatg cgcagttaag
     8521 tgatgtttcg cgctgtttta ccgactggca acaggttcag gaagatatcg aaaccgcaca
     8581 gatgatgctc gatgatcctg aaatgcgtga gatggcgcag gatgaactgc gcaaagctaa
     8641 agaaaaaagc gagcaactgg aacagcaatt acaggttctg ttactgccaa aagatcctga
     8701 tgacgaacgt aacgccttcc tcgaagtccg agccggaacc ggcggcgacg aagcggcgct
     8761 gttcgcaggc gatctgttcc gtatgtacag ccgttacgcc gaagcccgcc gctggcgggt
     8821 agaaatcatg agcgccagcg agggtgaaca tggtggttat aaagagatca tcgccaaaat
     8881 tagcggtgat ggtgtgtatg gtcgtctgaa atttgaatct ggcggtcatc gcgtgcagcg
     8941 tgttcctgct acggaatcgc agggtcgtat tcatacttct gcttgtaccg ttgcggtaat
     9001 gccagaactg cctgacgcag aactgccgga catcaaccca gcagatttac gcattgatac
     9061 tttccgctcg tcaggggcgg gtggtcagca cgttaacacc accgattcgg caattcgtat
     9121 tactcacttg ccgaccggga ttgttgttga atgtcaggat gaacgttcac aacataaaaa
     9181 caaagcgaag gcgctttcag tgctcggtgc tcgcatccac gctgctgaaa tggcaaaacg
     9241 ccaacaggcc gaagcgtcta cccgtcgtaa cctgctgggg agtggcgatc gcagcgaccg
     9301 taaccgtact tacaatttcc cgcaggggcg cgttaccgat caccgcatca acctgacgct
     9361 ctaccgcctg gatgaagtga tggaaggtaa gctggatatg ctgattgaac cgattatcca
     9421 ggaacatcag gccgaccaac tggcggcgtt gtccgagcag gaataatgga atatcaacac
     9481 tggttacgtg aagcaataag ccaacttcag gcgagcgaaa gcccgcggcg tgatgctgaa
     9541 atcctgctgg agcatgttac cggcagaggg cgtactttta ttctcgcctt tggtgaaacg
     9601 cagctgactg acgaacaatg tcagcaactt gatgcgctac tgacacgtcg tcgcgatggt
     9661 gaacccattg ctcatttaac cggggtgcga gaattctggt cgttgccgtt atttgtttcg
     9721 ccagcgacct taattccgcg cccggatacg gagtgtctgg tggagcaggc actggcgcgg
     9781 ttgcctgaac aaccttgccg tattcttgat ctcgggacgg gtaccggggc gattgcgctt
     9841 gcgctggcta gcgagcgccc ggactgcgaa attatcgctg tagatcgtat gcctgatgct
     9901 gtctccctgg cacaacgtaa tgcccagcat ctggcgatca aaaatatcca cattctgcaa
     9961 agcgactggt ttagcgcgct agccgggcag cagtttgcga tgattgtcag caatccgccg
    10021 tatattgacg agcaggaccc acatcttcaa caaggcgatg tccgctttga gccgctcact
    10081 gcgctggttg cggcagacag tggaatggca gacatcgtgc atatcatcga acagtcgcgt
    10141 aacgcgctgg tatccggcgg ctttctgctt ctggaacatg gctggcagca gggcgaagcg
    10201 gtgcgacaag catttatcct cgcggggtat catgacgtcg aaacctgccg tgactatggt
    10261 gataacgagc gcgtaacgct cggccgctat tatcaatgac aagtttttct acactgctta
    10321 gtgttcatct tattagtatc gcgctttctg ttgggctatt aaccttacgt ttctggctac
    10381 gttatcagaa gcatccacag gcatttgctc gctggacgcg cattgtgccg ccggttgtcg
    10441 atacggtgtt attgttaagc ggcattgcgt tgatggctaa agcgcacatc ctgccatttt
    10501 ccgggcaggc acagtggctg actgaaaagc tgtttggagt tatcatttat atcgttttgg
    10561 gttttattgc actcgattat cgtcgtatgc acagtcagca ggcgcgcatt attgccttcc
    10621 cgctggcgtt ggtggtgctg tacatcatca ttaaactcgc caccacaaaa gtaccgttac
    10681 tggggtaagt catgagatcg ttagctgatt tcgaatttaa taaagcgcca ttgtgcgaag
    10741 gcatgatcct ggcttgcgaa gcaatccgcc gcgattttcc ctcgcaagat gtttacgacg
    10801 aactggagcg tctcgttagt ctggcgaagg aagaaatcag ccagcttctg cctttagaag
    10861 agcagctgga aaaactgatc gcgctgtttt acggcgactg gggatttaaa gcctcacgcg
    10921 gcgtttatcg tctttccgat gcattatggc tggaccaggt gttaaagaat cgacagggca
    10981 gtgcggtatc attaggtgcg gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc
    11041 tgccggtgat tttccctacg cagctgatat tgcgcattga atgtccggat ggcgaaattt
    11101 ggctgattaa tccttttaac ggtgaatcgt taagcgaaca tatgctggac gtatggttaa
    11161 agggaaatat cagcccgtcg gcggaactgt tttatgaaga ccttgatgaa gctgataaca
    11221 ttgaggtaat ccgcaaattg ctggatacac tcaaagcctc gttgatggaa gaaaatcaga
    11281 tggagctggc gttacgcacc agcgaagctt tattacaatt caaccctgaa gatccctagg
    11341 aaattcgcga tcgcgggttg atttatgcgc aactggattg cgaacacgtt gcgttgaacg
    11401 atttaagtta tttcgttgaa cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc
    11461 aaataaataa catcgcgcat aaacatattg tgctgcatta attaatcgac attttactca
    11521 agattaaggc gatcctatga aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa
    11581 tgacctgccg ttcgtactgt ttggcggtat gaacgtgctg gaatctcgcg atctggcgat
    11641 gcgcatttgc gagcactacg taactgtgac ccagaaactg ggtatccctt acgtgttcaa
    11701 agcctctttt gacaaagcca accgctcctc catccactct tatcgtggac cgggcctgga
    11761 agaagggatg aaaatcttcc aagagttgaa gcagactttt ggcgtgaaaa ttatcaccga
    11821 cgttcacgaa ccaagtcagg cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc
    11881 ggcgtttctt gctcgccaga ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat
    11941 taacgtcaag aaaccacagt ttgtcagccc gggacagatg ggtaatatcg ttgataaatt
    12001 caaagaaggc ggcaacgaaa aagtgattct ttgcgatcgt ggtgctaact tcggctatga
    12061 caacctggtt gtcgatatgc tgggcttcag cattatgaag aaagtgtctg gtaactcgcc
    12121 ggtgattttc gacgtgaccc acgcactgca atgccgcgat ccgtttggcg cagcttccgg
    12181 tggtcgtcgt gctcaggt
//
LOCUS       DADLTD010000126        12171 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLTD010000126 DADLTD010000000
VERSION     DADLTD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.126"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..2409
                     /gene="napA"
                     /locus_tag="JN701_002742"
     CDS             <1..2409
                     /gene="napA"
                     /locus_tag="JN701_002742"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY7365142.1"
                     /translation="GVARAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQQGRVVACQGD
                     PDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTLITWDQAFDVME
                     EKFKTALKEKGPESIGMFGSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCMASAVVG
                     FMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVAVLST
                     YQHRSFELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYG
                     LRPTHPLEKAAKNPGSDASEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYA
                     DPNKKVISYWTMGFNQHTRGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAR
                     EVGTFAHRLPADMVVTNEKHRDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVY
                     WTMCTNNMQAGPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGA
                     YGNAERRTQFWRQQVQAPGEAKSDLWQLVQFSRRFKTEEVWPEDLLAKKPELRGKTLY
                     EVLYATPEVSKFPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDY
                     HKARGLRWPVVNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAP
                     DEEYDLWLSTGRVLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKV
                     VSRRGEVISIVETRGRNRPPQGLVYMPFFDAAQLVNKLTQDATDPLSKETDFKKCAVK
                     LEKV"
     gene            2416..3111
                     /gene="napG"
                     /locus_tag="JN701_002743"
     CDS             2416..3111
                     /gene="napG"
                     /locus_tag="JN701_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY7365143.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            3098..3961
                     /gene="napH"
                     /locus_tag="JN701_002744"
     CDS             3098..3961
                     /gene="napH"
                     /locus_tag="JN701_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY7365144.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            3958..4407
                     /gene="napB"
                     /locus_tag="JN701_002745"
     CDS             3958..4407
                     /gene="napB"
                     /locus_tag="JN701_002745"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY7365145.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            4417..5019
                     /gene="napC"
                     /locus_tag="JN701_002746"
     CDS             4417..5019
                     /gene="napC"
                     /locus_tag="JN701_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY7365146.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            5038..5655
                     /gene="ccmA"
                     /locus_tag="JN701_002747"
     CDS             5038..5655
                     /gene="ccmA"
                     /locus_tag="JN701_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY7365147.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            5652..6314
                     /gene="ccmB"
                     /locus_tag="JN701_002748"
     CDS             5652..6314
                     /gene="ccmB"
                     /locus_tag="JN701_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY7365148.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIC
                     IQ"
     gene            6356..7093
                     /gene="ccmC"
                     /locus_tag="JN701_002749"
     CDS             6356..7093
                     /gene="ccmC"
                     /locus_tag="JN701_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY7365149.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            7090..7299
                     /gene="ccmD"
                     /locus_tag="JN701_002750"
     CDS             7090..7299
                     /gene="ccmD"
                     /locus_tag="JN701_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY7365150.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            7296..7775
                     /gene="ccmE"
                     /locus_tag="JN701_002751"
     CDS             7296..7775
                     /gene="ccmE"
                     /locus_tag="JN701_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY7365151.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            7772..9715
                     /locus_tag="JN701_002752"
     CDS             7772..9715
                     /locus_tag="JN701_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7365152.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            9712..10269
                     /gene="dsbE"
                     /locus_tag="JN701_002753"
     CDS             9712..10269
                     /gene="dsbE"
                     /locus_tag="JN701_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY7365153.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            10266..11318
                     /locus_tag="JN701_002754"
     CDS             10266..11318
                     /locus_tag="JN701_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY7365154.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(11353..12000)
                     /gene="narP"
                     /locus_tag="JN701_002755"
     CDS             complement(11353..12000)
                     /gene="narP"
                     /locus_tag="JN701_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY7365155.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         2527 a         3084 c         3650 g         2910 t
ORIGIN      
        1 ggcgttgccc gcgccgttgt tggtcagcag gaagccatca aatgggataa agcgccgtgc
       61 cgtttctgcg gtactggttg cggcgttctg gtcggaacgc agcagggacg tgtggtggcc
      121 tgtcagggcg acccagacgc accggttaac cgtggcctga actgcattaa gggctatttc
      181 ctgcccaaga tcatgtacgg taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac
      241 ggtaaatatg acaaagaagg cgaatttacc ctaatcacct gggatcaggc cttcgatgtg
      301 atggaagaga aattcaaaac cgccctgaaa gaaaaagggc cggaatcgat cggtatgttc
      361 ggttctggtc agtggactat ctgggaaggt tatgccgcgt ccaagctgtt taaagcgggc
      421 ttccgttcga acaacatcga cccgaacgcg cgtcactgta tggcgtcggc agtagttggc
      481 tttatgcgta cctttggtat ggatgagccg atgggctgct atgacgacat cgagcaggct
      541 gacgcgtttg tgctgtgggg cgcaaacatg gcggagatgc acccgatcct ctggtcgcgc
      601 atcactaacc gtcgtctctc taatcagaac gtcaccgtgg cggtgctttc tacctaccag
      661 catcgtagct tcgagctggc ggataacggc atcatcttta cgccacagtc tgacctggtg
      721 atcctgaact acatcgccaa ctatatcatt caaaacaatg cgataaatca ggacttcttc
      781 agcaaacacg ttaacctgcg caaaggggcg acggacattg gctacggttt acgtccgacc
      841 catccgctgg aaaaagcagc gaagaatcca ggttctgacg cctccgaacc gatgagcttt
      901 gaagattaca aagccttcgt tgccgagtat acgctggaaa aaactgccga aatgaccggc
      961 gtgccgaaag accagttaga acaactggcg cagctgtatg ccgatccgaa caagaaagtc
     1021 atctcctact ggacgatggg cttcaaccag catactcgtg gcgtgtgggc taacaacctg
     1081 gtctacaacc tgcacctgct gaccggcaaa atttcccagc cgggttgcgg tccgttctcc
     1141 ctgaccgggc aaccttctgc gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt
     1201 ctgcctgcgg acatggtggt gaccaacgag aaacaccgtg atatctgcga gaagaagtgg
     1261 aatatcccga gcggcaccat tccggcgaaa atcggtctgc acgcggtggc acaagaccgt
     1321 gcgctgaaag acggcaaact gaatgtttac tggaccatgt gtaccaacaa catgcaggcc
     1381 gggccgaaca tcaatgaaga gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc
     1441 gtctccgatc cgtatccgac agtcagtgcg ctggccgccg acttgatcct gccgaccgca
     1501 atgtgggtag agaaagaggg cgcttacggt aacgccgaac gccgtactca gttctggcgt
     1561 caacaggtac aggcaccggg cgaagcgaaa tcggatctct ggcagttagt ccagttctcc
     1621 cgccgcttca aaactgaaga agtatggccg gaagatctgc tggcgaagaa accggaactg
     1681 cgtggcaaaa cgctgtacga agtgctgtat gccacaccgg aagtgagcaa attcccggta
     1741 tccgaactgg cggaagatca gctgaacgat gaatcccgcg agctgggctt ctatctgcaa
     1801 aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc acggtcacga tctggcaccg
     1861 ttcgatgact accacaaagc gcgcggtctg cgctggccgg tggtgaacgg taaagaaacg
     1921 cagtggcgtt acagcgaagg taacgacccg tacgtgaaag cgggcgaagg ctataagttc
     1981 tacggcaaac cggatggcaa agcggtgatc ttcgcactgc cattcgaacc ggcggcggaa
     2041 gcaccggatg aagagtacga cctgtggctc tctaccggac gcgttctgga gcactggcac
     2101 accggcagta tgactcgccg tgtaccagaa ctgcaccgcg ccttcccgga agcggtcctg
     2161 tttattcacc cgctggatgc gaaagcgcgc gatctgcgtc gtggcgacaa agtgaaagtg
     2221 gtttctcgcc gtggcgaagt gatctcgatt gtcgaaacgc gcggtcgtaa ccgtccgccg
     2281 cagggcctgg tgtacatgcc gttcttcgac gccgcacagc tggttaacaa actaacgcag
     2341 gacgcgaccg atccgctctc gaaagagacg gacttcaaga agtgcgcagt caaactggag
     2401 aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg
     2461 cgcgatgttg ttcgcacagc aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa
     2521 cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac
     2581 gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc
     2641 ttaaaactgg cgacgctggc ctctggtctg tcggcgggca cgccatattt tgtcgcacgg
     2701 gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca aagtgtgccc gagcggtgcg
     2761 ctggatcgtg agatcgaatc gatcgacgac gcgcggatgg ggctggcggt actggtggac
     2821 caggaaaact gtctcaactt tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg
     2881 aaaattgatg aggccatcac cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc
     2941 cgctttctgc cgacggttca cagagacgcc tgtaccggtt gcggtaagtg cgaaaaagtg
     3001 tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt cactggctaa aggggagtta
     3061 ggtcaccatt accgcttcgg ctggctggag gggaacaatg gcaaatcgta aacgtgacgc
     3121 cgggcgcgag gcgctggaga aaaaaggctg gtggcgcagt caccgttggc tggtgttacg
     3181 tcgcctttgt cagttcttcg tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg
     3241 gatcttacac ggtaactaca gcagtagcct gttattcgac accgttccgc tgaccgatcc
     3301 gctgatgacg ctgcaaagtc ttgccagcgg tcatctgcct gccacggtgg cgttgaccgg
     3361 tgcggtgatt atcaccgtgc tctacgccct ggcaggaaag cgattatttt gcagctgggt
     3421 ctgcccgctg aatccgatta ccgacttagc gaactggtta cgcaggcgct ttgacctcaa
     3481 tcagtctgcg acgatccctc gccacattcg ctacgtgctg ttggtggtca ttctgctggg
     3541 atcggcctta actggcacgc tcatctggga atggatcaac cccgtatctc tgatggggcg
     3601 cagcctggta atgggcttcg gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga
     3661 tctactggtc gttgaacacg gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg
     3721 cgtgctgggt agcaaaggtg tgattaccgt tgcggccacc gatcggcaga aatgtaaccg
     3781 ctgtatggat tgttttcatg tttgcccgga accgcgtgtg ctacgtgccc cggtgctgga
     3841 tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga
     3901 tgtctgttct gaggatgtat ttacaataac tacacgatgg agttcgggag cgaaatcatg
     3961 aaaagccatg acctgaagaa agcgctgtgt caatggacgg cgatgctggc cctggtggta
     4021 agcggcgcgg tttgggcggc taatggcgtc gattttagcc agtcgccgga agtatccggg
     4081 acacaggaag gggccattcg gatgccaaaa gagcaggaac ggatgccgct gaactatgtg
     4141 aatcagccgc caatgatccc gcatagcgtt gaaggttatc aggtaacgac caataccaac
     4201 cgctgcttgc agtgccacgg tgtcgaaagt tatcgcacca ctggcgcgcc gcgcattagt
     4261 cctactcact ttatggacag cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat
     4321 ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc caatcgtggg gaataccttt
     4381 accccatcaa aaggttacgg gaaataagag gttattatgg gaaattctga ccgtaagcct
     4441 ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca gccgtctggc gctggggacg
     4501 ctgctgttga tcggttttgt tggcggcatc gtcttctggg gtggctttaa caccgggatg
     4561 gaaaaagcca ataccgaaga gttctgcatt agctgccacg aaatgcgcaa cacggtgtat
     4621 caggaataca tggattccgt gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg
     4681 gattgtcacg ttccgcacga gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa
     4741 gagctgtatg gtaaaatttt tggcgttatt gacacgccgc agaaatttga agctcatcgt
     4801 ctgacgatgg cacagaatga gtggcggcgc atgaaggaca ataactcgca ggagtgccgt
     4861 aactgtcaca acttcgagta tatggataca accgcccaga aatcggttgc cgcgaagatg
     4921 catgaccagg cggtgaaaga tgggcaaacc tgtattgatt gccataaagg gatagcgcac
     4981 aagctgcccg atatgcgtga agtcgagcca ggtttttaac agggttattg cgtgggtatg
     5041 cttgaagcca gagagttact ttgtgagcgg gatgaacgaa ccttatttag tggcttgtca
     5101 tttacgctga acgcaggaga gtgggtacaa atcaccggta gcaacggcgc ggggaagaca
     5161 acgcttctcc gtttgctgac ggggttgtct cgccctgacg caggcgaggt tctctggcaa
     5221 gggcagccct tgcatcaggt acgcgacagc taccatcaaa acctgttatg gataggccat
     5281 cagccgggga tcaaaacccg gctgacggcg ttagaaaatc tgcactttta tcatcgcgat
     5341 ggcgataccg cacaatgtct ggaagccctg gcgcaggccg ggcttgccgg attcgaagat
     5401 attcctgtaa atcagctctc ggccgggcaa caacgccgcg tcgctttagc gcgtctgtgg
     5461 ctgacccgtg ccacgttatg gatcctcgac gagcctttta ccgcgattga cgttaacggc
     5521 gtcgatcgtc tgacccagcg tatggcgcag catacggagc agggggggat tgtgattctg
     5581 actacccacc agccgctcaa cgttgctgaa agtaaaattc gccgcatttc actgacgcaa
     5641 acgagggccg catgatgttc tggcgcattt tccgtcttga gctgcgtgta gcgtttcgcc
     5701 atagcgccga aatcgccaac ccgctgtggt tcttcctgat tgtaattacc ctttttccgc
     5761 tcagtatcgg tccggagccg caactgctgg cgcgtattgc accgggcatt atctgggttg
     5821 ctgcgctgct ttcatccttg ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg
     5881 gcagtcttga acaattgatg ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg
     5941 tgatggcgca ctggatggta accggtctgc cgttactcat cctttcgcca ctggtagcaa
     6001 tgctactggg aatggatgtt tatggctggc aagtgatggc gctgacgctg ctgctgggaa
     6061 cgcctacgct tggctttctc ggtgcaccgg gtgtggcgtt gacagtggga cttaagcgcg
     6121 gtggtgtgct gctcagcata ctggtgttac cgctgactat cccattactc atctttgcca
     6181 ccgccgcgat ggacgcggct tccatgcatt tgcccgttga cgggtatctg gcaattttag
     6241 gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc gacggcggca gcgttacgaa
     6301 tctgcattca ataacgctgg attactgatt tttgttcgag tctggtatcg aaactatgtg
     6361 gaaaacactg catcaactgg cgatcccacc acggctgtat caaatctgtg gctggtttat
     6421 accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg
     6481 ctttgctccg gctgattatc agcagggaaa tagctaccgc attatctacc tgcatgtgcc
     6541 tgcggcgatc tggtcgatgg gtatttatgc atcaatggcg gtggcggcgt ttattggcct
     6601 tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg atggccccca ttggtgccgt
     6661 gtttaccttt attgccctgg ttaccggctc tgcatgggga aaaccgatgt ggggcacctg
     6721 gtgggtatgg gatgcacgtc tgacttctga actggtgctg ctgtttttgt atgtgggtgt
     6781 gattgccctg tggcacgcct tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct
     6841 ggtgctgatt ggcgtggtga atctgccgat tattcattac tccgtggagt ggtggaacac
     6901 cctgcatcag ggatcaacgc ggatgcagca aagtatcgat ccggcgatgc gttcgccgct
     6961 gcgctggtcg atttttggct tcctgctcct gtctgccacg ctgacgctga tgcggatgcg
     7021 taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg agtgaactga tactgaaaag
     7081 aggccgtaaa tgacccttgc atttgcttcc tggaatgaat ttttcgcaat gggcggttac
     7141 gccttttttg tctggctggc ggtggtgatg accgttattc cgctggtggt tttggtcgtg
     7201 cactcggtga tgcagcatcg cgcaattctg tgtggtgtgg agcaacagcg ggcgcgtgag
     7261 gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa tattcgccgt aaaaaccgct
     7321 tgtggattgc ctgtgctgtg ttggcaggtt tggcgctgac catcggtctg gtgctgtatg
     7381 cgctgcgctc gaatatcgat ctcttttata cgccggggga aattctctac ggcaagcgtg
     7441 aaactcagca aatgccggaa gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg
     7501 gtagtgtgca gcgcgatccc aattcgctga aagtgacctt caccatttac gacgctgaag
     7561 gctcagtgga tgtctcttac gaaggcattt tgccggatct gttccgtgaa gggcagggcg
     7621 ttgtggtgca gggcgagctg gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga
     7681 aacacgacga aaactacacg ccgccagaag ttgagaaagc gatggaagct aaccaccgtc
     7741 gcccggcgag tgtttataag gacccagcat catgatgccg gaaattggaa acgggctgct
     7801 gtgcctggcg ctgggaattg cgctgctgct gtccgtgtat ccgctatggg gcgtggcgcg
     7861 cggagatgcg cgcatgatgg cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt
     7921 ggctggcgca tttctggtgc tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta
     7981 tgttgccagc aactccaata cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg
     8041 cgcgcatgaa ggttcgcttc tgctgtgggt gctgctgatg agcggctgga cctttgcggt
     8101 ggcgattttt agtcagcgta ttccgctgga tattgtggcc cgtgtactgg cgataatggg
     8161 gatggtcagc gtcggctttt tgctgttcat tctctttacc tctaacccgt tctcacgcac
     8221 gctgccgaac ttcccaattg aagggcgcga tcttaacccg ctgttacagg atccggggct
     8281 gatcttccat ccgcctctgc tttatatggg gtacgtgggt ttctcggtgg cgtttgcttt
     8341 tgccattgct tctttgctga gcgggcgtct ggacagcact tatgcgcgtt ttactcgccc
     8401 gtggacgctg gcggcgtgga ttttcctgac gctcggcatt gtgctcggtt ccgcatgggc
     8461 ctattacgaa ctcggctggg ggggctggtg gttctgggat ccggtagaaa acgcctcgtt
     8521 tatgccgtgg ctggtgggga ctgcgctgat gcactcactg gcggtcactg aacaacgcgc
     8581 cagcttcaaa gcgtggacat tactgctggc aatcagtgcc ttctcgttgt gtctgctggg
     8641 gactttcctg gtgcgttccg gcgtgctggt atcggtacac gcgtttgcct ctgatccggc
     8701 acgcggtatg tttatcctcg cctttatggt actggtgatt ggtggttcgc tgctgctgtt
     8761 tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc
     8821 tctgctgtta gcgaacaacg ttttgttggt cgccgcgatg ctggtggtat tactggggac
     8881 gctgctgccg ctggtgcaca agcaactggg actgggcagt atttcgattg gcgaaccgtt
     8941 cttcaacacc atgtttacct ggctgatggt gccgtttgcg ctgctgcttg gtgtcggtcc
     9001 tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc aatttattga ttatcgcctt
     9061 catctcaacg ctggtgctgt cgctgctgct gccgtggctg ttcgaaagca aagttgtggc
     9121 gatgacggtg ctcggcctgg caatggcctg ctggattgct gtgctggcaa ttgcggaagc
     9181 tgcgctgcgt atttcacgtg gcacgaaaac caccttcagt tattggggaa tggtggcggc
     9241 tcacctgggg ctggcagtga caattgttgg tattgccttt agccagaact atagcgttga
     9301 gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt catgaatatc gcttcacctt
     9361 ccgtgatgtc aaagaggtga ctggcccgaa ctggcgcggc ggtgtggcga ctattggcgt
     9421 aacgcgagag ggtaaaccgg aaacggtgct gtatgcggaa aaacgttatt acaacactgc
     9481 cgggtcgatg atgaccgaag cggcgattga cggcggcatc acgcgtgacc tgtacgccgc
     9541 gctcggtgaa gagctggaaa acggcgcgtg ggccgtgcgt ctttactaca aaccgtttgt
     9601 tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc ggactgctgt gtctgtttga
     9661 tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg ccggaggctg tatgaagcgc
     9721 aaagtattgt taattccgtt gattatcttc ctggcgattg ccgcggcgct gctgtggcag
     9781 ctggcgcgta atgccgaagg ggatgatccg accaatctgg aatcggcgct cattggcaag
     9841 ccagtgccga agtttcgcct cgaatcactg gacaatccgg ggcagtttta tcaggcggat
     9901 gtgctgactc agggcaaacc tgtgctgctt aacgtttggg ctacctggtg tccgacctgc
     9961 cgtgcggaac atcaatatct gaatcagctt tctgcgcagg gcattcgcgt ggtcggcatg
    10021 aactataaag acgatcgcca gaaggcaata agctggctga aagagctggg caatccttac
    10081 gcgctaagcc tgtttgatgg cgatggcatg ttagggctgg atctcggtgt ctatggcgcg
    10141 ccagaaacgt tcctcatcga cggcaacggc atcattcgct atcgccatgc gggcgatctc
    10201 aatcctcgcg tctgggaaga agagatcaag ccgctgtggg agaaatacag taaggaggcc
    10261 gcacaatgag gtttttattg ggcgtgctga tgctgatgat ctccggctca gcgctggcga
    10321 ccatcgacgt gttgcagttt aaggatgaag cgcaggagca gcagttccgc caactcactg
    10381 aagaactacg ctgcccgaaa tgccagaaca acagcattgc cgattccaac tcgatgattg
    10441 ccaccgacct gcgccagaaa gtgtatgaac tgatgcagga aggtaaaagt aaaaaagaga
    10501 ttgtcgatta tatggtggcg cgttacggca acttcgtcac ttacgatccg ccgttaacgc
    10561 cgctgaccgt gctgctgtgg gtgcttccgg tagtggctat tggcattggc ggttgggtca
    10621 tttacgcccg ttcgcggcgt cgggtacgcg tggtgccgga cgcgtttcct gaacaaagcg
    10681 tgccggaagg taagcgtgcc ggatatattg tttatctgcc gggtattgtg gtggcgttaa
    10741 ttgtggctgg cgtcagctac taccagactg gcaattatca gcaggtgaaa atctggcagc
    10801 aggccacggc acaggctccg gcgttgctgg acagggcgct ggatccgaaa gccgatccgc
    10861 tcaacgaaga agagatgtcg cgtcttgcgc tggggatgcg tactcaactg caaaaaaatc
    10921 cgggagatat agaaggctgg attatgttgg gccgcgttgg catggcgctg ggtaacgcca
    10981 gtatcgccac cgatgcatac gctactgcgt atcgcctcga tccgaaaaac agtgatgccg
    11041 cactgggtta tgctgaagcg ttgacacgtt catctgatcc caacgacaac cgcctcggtg
    11101 gtgaactgct gcgccagttg gtgagaacgg accatagcaa tatccgtgtg ttaagcatgt
    11161 atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc cgttgccgcg tgggagatga
    11221 tgttgaaact cttatctgcc aacgatactc gccgtgcggt gattgaacgt agtatcgcgc
    11281 aggcgatgca acatttgtcg ccgcaggaga gtaaataaaa agaggcccga tggtgatgcc
    11341 atcgggctat ttttattgtg ccccgcgttg ttgcaggaac agaatggtgg ctgccacgcg
    11401 tgagcggaca ttgagtttgc gcagcagatt gcgaatatgt acttttactg tctgctctga
    11461 aatattcaac accgaggcaa tctgtttatt tgacagcccc tgtgccagct cgtgcagaac
    11521 atccagctcg cgctccgtca gcacgctgaa gggatcttct tccgcgccaa acatttcacg
    11581 ttcacgtaag tactgattga cgcgttcgct aaagactttg ctgcctttcg ctccagcacg
    11641 aattgcttcc agcaatactt ccgggtcgct gtctttcaac agataaccgt ctgcgcctgc
    11701 gtctatcagc gcaaagacat cgctggaggc atctgatacg gtcaggataa taatttgcgc
    11761 ggtaacgcca tccctgcgca aggcattgag ggtatccagg ccactcatac ctttcatatt
    11821 gagatccagc aagatcacgt cgatatccag tctatttgcc agatcgatcg cgctcgcgcc
    11881 gtcgcccgct tcggcgacca cttcaaagct aggatcaagc tccagtaact gacgaacacc
    11941 gcgtcgcata agtggatgat catccacaat catcacctga aaaggcgttg cttcaggcat
    12001 agtagtctcc tggggtttta ttagaatcat tatgtttgag cgtagcgttg gtaacgctac
    12061 caggcgcgtg aatgttaccc cattttatgt atggacatgt agtgaaaact acaaccttgt
    12121 cgttcgcttt ggcatcattt tcattataaa gacggaattt tgcatcagct t
//
LOCUS       DADLTD010000127        12081 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLTD010000127 DADLTD010000000
VERSION     DADLTD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.127"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(59..1651)
                     /locus_tag="JN701_002756"
     CDS             complement(59..1651)
                     /locus_tag="JN701_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY7365156.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPGSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1870..2790
                     /gene="ldtB"
                     /locus_tag="JN701_002757"
     CDS             1870..2790
                     /gene="ldtB"
                     /locus_tag="JN701_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7365157.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2849..3967)
                     /locus_tag="JN701_002758"
     CDS             complement(2849..3967)
                     /locus_tag="JN701_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY7365158.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3964..4431)
                     /gene="mntR"
                     /locus_tag="JN701_002759"
     CDS             complement(3964..4431)
                     /gene="mntR"
                     /locus_tag="JN701_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HAY7365159.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGTK"
     gene            4617..4745
                     /gene="mntS"
                     /locus_tag="JN701_002760"
     CDS             4617..4745
                     /gene="mntS"
                     /locus_tag="JN701_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY7365160.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            5017..6600
                     /locus_tag="JN701_002761"
     CDS             5017..6600
                     /locus_tag="JN701_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7365161.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6649..7164)
                     /gene="ompX"
                     /locus_tag="JN701_002762"
     CDS             complement(6649..7164)
                     /gene="ompX"
                     /locus_tag="JN701_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY7365162.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7217..7282)
                     /locus_tag="JN701_002763"
     CDS             complement(7217..7282)
                     /locus_tag="JN701_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365163.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7517..8404
                     /gene="rhtA"
                     /locus_tag="JN701_002764"
     CDS             7517..8404
                     /gene="rhtA"
                     /locus_tag="JN701_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY7365164.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8703..9206
                     /gene="dps"
                     /locus_tag="JN701_002765"
                     /gene_synonym="pexB"
     CDS             8703..9206
                     /gene="dps"
                     /locus_tag="JN701_002765"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY7365165.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9610..10356
                     /gene="glnH"
                     /locus_tag="JN701_002766"
     CDS             9610..10356
                     /gene="glnH"
                     /locus_tag="JN701_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY7365166.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10495..11154
                     /gene="glnP"
                     /locus_tag="JN701_002767"
     CDS             10495..11154
                     /gene="glnP"
                     /locus_tag="JN701_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY7365167.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            11151..11873
                     /gene="glnQ"
                     /locus_tag="JN701_002768"
     CDS             11151..11873
                     /gene="glnQ"
                     /locus_tag="JN701_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY7365168.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         3024 a         3121 c         2916 g         3020 t
ORIGIN      
        1 ttttgcattt gtcatcagcc gccgtcaccc cgacagtatc ggggtgacgg ataatcactt
       61 actcgatccc tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt
      121 ccggggtgat ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa
      181 caaagatcag cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca
      241 tatccaggtg gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact
      301 taccaaacag catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt
      361 cgtcctggct gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt
      421 cctgcttcca ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt
      481 ggtcctgggc gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgcccg
      541 gttgcagatc gcccaccagc gttttcatca gcgttgattt accgacgccg ttggtaccca
      601 gtaccgcaag tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt
      661 tatcaaaccc tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct
      721 gttcaaaacg gatgaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt
      781 tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc
      841 ggctaacgaa agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca
      901 gacgttcacg cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc
      961 gcagctcgcc gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt
     1021 cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa
     1081 tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggatcagcaa
     1141 acagcgcctg cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc
     1201 cgtagtgctg ttccactgga attcccacgc caagcaacaa ttcaccggcg cgagcttccg
     1261 cagagtaacc gtccatttcg ccgtatttaa cttccagatc ggccacttta tagccgtctt
     1321 cctcactcat ttccggcaaa gcatagatgc ggtcacgctc ctgcttcact tcccacaact
     1381 ctttatgccc catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct
     1441 gacgcagctt accaatgcgc tcgttgggat cgagggaaac gttacccaaa gtcggctcta
     1501 agtcgccgcc gaggatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc
     1561 cgtaacggtt gccgccgcca aatttgacgg aaatgttttc aaacaacggc ttactgccga
     1621 actgcatggt gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa
     1681 ccgcgcatta tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca
     1741 agccaaaggc aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac
     1801 ttataatgcg cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc
     1861 tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc
     1921 ttttgcagta ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt
     1981 ggtcagaatc aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc
     2041 gcggagtacc agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc
     2101 ctgccgaaag gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt
     2161 catgaaggca tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc
     2221 aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac
     2281 tggaccacca aagttgagcg taagaaagca ggcccgacct ggacgccgac cgcaaaaatg
     2341 cacgcagagt accgcgctgc gggcgaaccg cttccggctg tcgttccggc aggtccggat
     2401 aacccgatgg gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac
     2461 gccaacttcg gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac
     2521 atcaaattcc tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg
     2581 gtaaaagcga ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct
     2641 accaccgaag cccagtttga aggtcaggaa attgtgccaa ttaccctgac gaagagcgtg
     2701 cagacagtga ccggtcagcc agatgttgac caggttgttc ttgatgaagc gattaaaaac
     2761 cgctccggga tgccggttcg tctgaattaa tcttcaaaac ttaaagcaaa aggcggactc
     2821 ataatccgcc ttttttattt gccagaccct agttggccgg gagtataact aacaaaacat
     2881 atcccaccaa cgccgcccac aacagcatcg gtattgaata gagatggaaa cgccaccaga
     2941 tgcggcgatc gttcgccata cgtagcgcaa tcaaacttgc cagcgatccg ggtaataacc
     3001 caaagccacc tacgtttacc gcccatgcca gtaataaaga cggcggcaca tagttcagca
     3061 acaatatggt actcggcacg ttactgatca cctgcgataa accgattgcc gttaaccata
     3121 acccgggttc agatagatga ctcacgttac ccaacacgcc ttgcaacgct ggaagctggg
     3181 tcagtaaatg gacgtcgata aacatcgcca taaacaccag cagcagcgtc cagtccacac
     3241 tgagcaccac gcgacgtgcc agcagcgcaa agcctgccgc cacaatcacc agtccccaca
     3301 gctcttgttt gaactccagc gccgttagaa agacgatata cagccccaga caactccaca
     3361 ccagccgcgg tttccactcc ggtgtttgca cccccgtatg gtattgcagc acctttccag
     3421 ggaaacaaca ccagcacagg agcaggagcg tcagcatcat tgcgccagcc agcggtgcca
     3481 tttgggcaat aaatccggca aacgaaagac cagaacgtcc ccagataaga atattttgcg
     3541 ggttgccaat tggcgtcagt agcgaaccag cattgactgc cagcgcctca aaaataatca
     3601 gacgattaac cgggatctca cacagtcttt ttagcgtgat agtcagcgga acaacaataa
     3661 acagcgcgac atcgttggtc agaaaggtag aaagcagcgc cgccgccagc accataaaca
     3721 tcgccagccg acgctccgta gcaaagcggc gcaccatttt gcgccccagc acatcaaaat
     3781 aaccgcttaa ctccacacct ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt
     3841 gccagtcgat agcagcaggc caggatttcg gtgcaaaggg cacgaaaaag cttaatccga
     3901 taccaacaag aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca
     3961 ggctcatttg gtaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg tctcttcact
     4021 aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac
     4081 caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc
     4141 ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag
     4201 ccgcttaagc attttagcca ccgtcggttg cgaaactccc agacgagcag ccatgtccac
     4261 ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat
     4321 aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt
     4381 cactaactgc gtcacttttt ttgctgttgg cgtacctgcg cgacgactca ttgtgcttcc
     4441 tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg
     4501 cgaaggtaca aaaaattaac gttttagcaa tagctatata atatagcctg tgctatatct
     4561 gtatgtaatg caatcatccc tcaaggatcg acgggattag caagtcagga ggtcttatga
     4621 atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatac
     4681 tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaagataaa ccttccgata
     4741 aatagcggcg tcgcggtacg ccgcttcact cctgctttca tgcaggcata acgcgttttg
     4801 gcctgaaaaa ccccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc
     4861 agtagttgac ctgaacggcg gctcgctcta tcttcttgca gccctgcgta tattgcggct
     4921 cgcggatgcg gacccctttc cactcttcac gcactcttgc aggtattgac ccttgacgcc
     4981 agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg
     5041 cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta
     5101 ttaattaacc tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct
     5161 attttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtgtcagt
     5221 tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc taatttcaat
     5281 acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt
     5341 ccgtggtaca gctacctggt cggcttattt atttttgcgc tcggcgtaat agcaatcagg
     5401 cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg acagcctgtg tctggtattc
     5461 agtgtggcga cattttttgt tgctcccgtg caaaacctgg cctggggtgg cgtatttaaa
     5521 ctgaaagata ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac
     5581 gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg
     5641 gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg tgatcggtga aagcgcgcgt
     5701 cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt
     5761 aacggtttga tatttgctga ctacattgcc gccagtggct ccacgcagaa atcgcttggc
     5821 ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc aggataactt tgtcaccctg
     5881 gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggccaaat cggcgaatac
     5941 gatactgcta tcgccagcat cgccaaacgt gcagatgaag tgtacttcct gaaagaaggt
     6001 aattttgaag cagataaaaa cacgaaagac gaagcgttac tggatatgac cgctcaagtg
     6061 ctggcgcaag agcactcgca accgcagctg attgttctgc atctgatggg ctcgcatccg
     6121 caggcctgcg acaggacaca aggcaaatac gaaacctttg tgcaatcgaa agaaacgtcg
     6181 tgctatctct ataccatgac gcaaacggac gatttactgc gcaagctgta cgatcagtta
     6241 cgcaacagcg gcagcagctt ctctctggtt tacttttctg accacggtct ggcctttaaa
     6301 gagcgcggca aagacgtgca ataccttgcc catgatgata aatatcagca aaatttccag
     6361 gtgcctttta tggtcatttc cagcgacgat aaagcgcatc gggtgattaa agcccgccgc
     6421 tcagccaatg acttcttagg ctttttctcg cagtggacgg gaattaaagc gaaggaaata
     6481 aatatcaaat acccgtttat atctgagaag aaagccgggc cgatatacat caccaacttc
     6541 cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc gaaaccttaa
     6601 aaacaaaaat ccgccccgag aggcggattt tttatatcac caaagtgatt agaagcggta
     6661 accaacaccg gcaatccagg tgcctacgtc aacgctacga atacggctct gctcgtaaga
     6721 gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga ccagcaccgt aggagaaacc
     6781 gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg gtctggaatt taccataacc
     6841 cacacccact acaccgtaga tgcttgccca gtcgttaatg cggtaagccg gaccagcagt
     6901 gatgccgtag tactggtttt tgttgtagtc accagagctt gcagtacgac ttttctcggt
     6961 gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct tcttcatagc ggtatttcag
     7021 gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg ctctgtgcgt aaccgccagt
     7081 tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa gccagaactg cggccagtgc
     7141 tgaaagacat gcaatttttt tcataaccac ctcaaatgtg attcaaataa gtcctaagtt
     7201 ttaaatatat caaaaattaa tgggaaactc ttcgcgattt gtgatgtcta acgggccatt
     7261 tcatgtaaca gaacgtttcc atacaccgct atccatctaa atttaaatca ctttttcaga
     7321 gaactgcgta agtattacgc atgttttccc tgtcattcat ccagattatt cctaatcacc
     7381 agactaatga ttccatcaat cctggcgcat tttagtcaaa acgggggaaa attttttcaa
     7441 caaatgctca accagcattg ggtatatcca gtacactcca cgctttactt aagtctagat
     7501 atttgtggga gaaaggatgc ctggttcatt acgtaaaatg ccggtctggt taccaatagt
     7561 catattgctc gttgccatgg cgtctattca gggtggagcc tcgttagcta agtcactttt
     7621 tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg gcattaggaa cgctgatcct
     7681 catcgcgttc tttaagccat ggcgactgcg ctttgccaaa gagcaacggt taccgctgtt
     7741 gttttacggc gtttcgctgg gtgggatgaa ttatcttttt tatctttcta ttcagacagt
     7801 accgctgggt attgcggtgg cgctggagtt caccggacca ctggcggtgg cgctgttctc
     7861 ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg gttcttggtc tgtggttcct
     7921 gctaccgctg gggcaagacg tttcccatgt cgatttaacc ggctgtgcgc tggcactggg
     7981 ggccggggct tgttgggcta tttacatttt aagtgggcaa cgcgcaggag cggaacatgg
     8041 ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta attttcgtgc caattggagc
     8101 gcttcaggct ggtgaagcac tctggcactg gtcggttatt ccattgggtc tggctgtcgc
     8161 tattctctcg accgctctgc cttattcgct ggaaatgatt gccctcaccc gtttgccaac
     8221 acggacattt ggtacgctga tgagcatgga accggcgctg gctgccgttt ccgggatgat
     8281 tttcctcgga gaaacactga cacccataca gctgctggcg ctcggcgcta tcatcgccgc
     8341 ttcaatgggg tctacgctga cagtacgcaa agagagcaaa ataaaagaat tagacattaa
     8401 ttaaatttac atttctgcat ggttatgcat aaccatgcag aatttctcgc tacttttcct
     8461 ctacaccgtc tttatatatc gaattatgca aaagcatatt tattccgaaa attcctggcg
     8521 agcagataaa taagaattgt tcttatcaat atatctaact cattgaatct ttattagttt
     8581 tgtttttcac gcttgttacc actattagtg tgataggaac agccagaata gcggaacaca
     8641 tagccggtgc tatacttagt ctcgttaatt actgggacat aacatcaaga ggatatgaaa
     8701 ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa tctgctttat acccgcaacg
     8761 atgtctccga cagcgagaaa aaagcaacag tagagttgct gaatcgccag gttatccagt
     8821 ttattgatct ttctttgatt accaaacaag cgcactggaa catgcgcggc gctaacttca
     8881 ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact gatcgatcat ctggatacca
     8941 tggcagaacg tgcagtgcag ctgggcggtg tagctctggg gaccactcaa gttatcaaca
     9001 gcaaaacccc gctgaaaagt tacccgctgg acatccacaa cgttcaggat cacctgaaag
     9061 aactggctga ccgttacgca atcgtcgcta atgacgtacg caaagcgatt ggcgaagcga
     9121 aagatgacga caccgcagat atcctgaccg ccgcgtctcg cgacctggat aaattcctgt
     9181 ggtttatcga gtctaacatc gaataaatcc atcgctgatg gtgcagaact ttagtacccg
     9241 ataaaagcgg cttcctgaca ggaggccgtt ttgttttgca gcccacctca acgcacttat
     9301 ttagtgcatc catctgctat ctccagctga ttaagtaaat tttttgtatc cacatcatca
     9361 cacaatcgtt acataaagat tgttttttca tcaggtttta cgctaaataa tcactgtgtt
     9421 gagtgcacaa ttttagcgca ccagattggt gccccagaat ggtgcatctt cagggtattg
     9481 ccctataaat cgtgcatcac gtttttgccg catctctaaa aatcaatgag ttgcaaaact
     9541 ggcacgattt tttcatatat gtgaatgtca cgcaggggat cgtcccgtgg atagaaaaaa
     9601 ggaaatgcta tgaagtctgt attaaaagtt tcactggctg cactgaccct ggtttttgcg
     9661 gtttcttctc atgccgcgga taaaaaatta gttgtcgcga cggataccgc cttcgttccg
     9721 tttgaattta aacagggcga taaatatgtg ggctttgacg ttgatctgtg ggctgccatc
     9781 gctaaagagc tgaagctgga ttacgaactg aagccgatgg atttcagtgg gatcattccg
     9841 gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca ttaccatcac cgacgagcgt
     9901 aaaaaagcga tcgatttctc tgacggctac tacaaaagcg gcctgttagt gatggtgaaa
     9961 gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg ggaaagtggt tgctgtgaag
    10021 agcggtactg gctccgttga ttacgcgaaa gcaaacatca aaactaaaga tctgcgtcag
    10081 ttcccgaaca tcgataacgc ctatatggaa ctgggcacca accgcgcaga cgccgttctg
    10141 cacgatacgc caaacattct gtacttcatc aaaaccgccg gtaacggtca gttcaaagcg
    10201 gtaggtgact ctctggaagc gcagcaatac ggtattgcgt tcccgaaagg tagcgacgag
    10261 ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg agaacggaac ttacaacgaa
    10321 atctacaaaa aatggttcgg tactgaaccg aaataataac gctacacctg taaagcgcac
    10381 tggcagttcc ctctcccctc tggggagagg attagggtga ggggcgcaaa cccgctccgg
    10441 ggccattaat taccctgaat ttgattattt acaccacggt aacaggaaca acatatgcag
    10501 tttgactgga gtgccatctg gcctgccatt ccgcttctga ttgaaggtgc caaaatgacc
    10561 ctgtggattt cggtcctcgg tctggctggc ggtctggtaa tcggattgct ggcaggtttt
    10621 gcacgcacct tcggtggttg gatagccaac cacgtcgcgc tggtctttat tgaagtgatc
    10681 cgcggcacac ctatcgtcgt ccaggtgatg tttatctatt tcgccctgcc gatggcgttt
    10741 aacgacttac gcatcgaccc atttactgcg gcggtggtca ccatcatgat caactccggc
    10801 gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta ttcacaaagg ttttcgtgaa
    10861 gcaggactgg cgctcggtct ttcacgttgg gaaaccattc gctacgtcat tttaccgctg
    10921 gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga tcatcagcat taaagacacc
    10981 tcgctgttta ttgttatcgg cgtggcggaa ctgacccgtc aggggcaaga aattattgcc
    11041 ggtaacttcc gcgcccttga gatctggagc gccgtggcgg tgttctatct gattattacc
    11101 ctggtgctga gctttattct gcgtcgtctg gaaagaagga tgaaaatcct gtgattgaat
    11161 ttaaaaacgt ctccaagcac tttggcccaa cccaggtgct gcacaatatc gatttgaaca
    11221 ttgcccaggg cgaagtcgtg gtgattatcg ggccgtccgg ttccggtaaa tcgaccctgc
    11281 tgcgctgcat caacaaactg gaagaaatca cctccggcga tctgattgtc gatggcctga
    11341 aggttaacga tccgaaagtt gacgagcgcc tgattcgcca ggaagcaggt atggtgttcc
    11401 agcagtttta cctcttcccg catctgacgg cgctggaaaa cgtcatgttt ggtccgctac
    11461 gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc gcgtgagctg ctggcgaaag
    11521 tcggtctggc agaacgtgca catcactacc cttccgaact ttctggtggt caacagcagc
    11581 gtgtggcgat tgcccgcgcg ctggcggtga agccgaaaat gatgctgttt gatgaaccga
    11641 cttccgctct tgacccggaa ctgcgccatg aagtgctgaa ggttatgcag gatctggctg
    11701 aagaagggat gacgatggtg atcgtgaccc acgaaatcgg ttttgccgag aaagtagctt
    11761 cgcggctgat ctttatcgac aaaggccgga ttgcggaaga tggcaatccg caggtgttga
    11821 tcaagaaccc gccgagccag cgcttgcagg aatttttgca gcacgtctct taataagaca
    11881 cattgcctga tgcgctacgc ttatcaggcc tacaggatat ctggcaactt attaaaattg
    11941 catgaacttg taggacggat aaggcgttca cgccgcatcc ggcaaaaaag cccgcacgtt
    12001 gtcagcaatc tgcttaatat cccttcctcc ctttcacccg aaagggaggc acaccagatt
    12061 cctctcattt aaaatcgccc c
//
LOCUS       DADLTD010000128        12040 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLTD010000128 DADLTD010000000
VERSION     DADLTD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.128"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..410)
                     /locus_tag="JN701_002769"
     CDS             complement(<1..410)
                     /locus_tag="JN701_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7365169.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDGLSSV"
     gene            566..1606
                     /gene="gpr"
                     /locus_tag="JN701_002770"
     CDS             566..1606
                     /gene="gpr"
                     /locus_tag="JN701_002770"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY7365170.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGYGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1646..2140)
                     /locus_tag="JN701_002771"
     CDS             complement(1646..2140)
                     /locus_tag="JN701_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY7365171.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2331..3215
                     /gene="yghA"
                     /locus_tag="JN701_002772"
     CDS             2331..3215
                     /gene="yghA"
                     /locus_tag="JN701_002772"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7365172.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3487..3912)
                     /gene="exbD"
                     /locus_tag="JN701_002773"
     CDS             complement(3487..3912)
                     /gene="exbD"
                     /locus_tag="JN701_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY7365173.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3919..4653)
                     /gene="exbB"
                     /locus_tag="JN701_002774"
     CDS             complement(3919..4653)
                     /gene="exbB"
                     /locus_tag="JN701_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7365174.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4905..6092
                     /gene="metC"
                     /locus_tag="JN701_002775"
     CDS             4905..6092
                     /gene="metC"
                     /locus_tag="JN701_002775"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY7365175.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6232..6891
                     /locus_tag="JN701_002776"
     CDS             6232..6891
                     /locus_tag="JN701_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7365176.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6931..7830)
                     /locus_tag="JN701_002777"
     CDS             complement(6931..7830)
                     /locus_tag="JN701_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7365177.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8024..9187
                     /gene="yqhD"
                     /locus_tag="JN701_002778"
     CDS             8024..9187
                     /gene="yqhD"
                     /locus_tag="JN701_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY7365178.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9292..10119
                     /gene="dkgA"
                     /locus_tag="JN701_002779"
     CDS             9292..10119
                     /gene="dkgA"
                     /locus_tag="JN701_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY7365179.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            10319..11245
                     /locus_tag="JN701_002780"
     CDS             10319..11245
                     /locus_tag="JN701_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY7365180.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11296..11553
                     /gene="yqhH"
                     /locus_tag="JN701_002781"
     CDS             11296..11553
                     /gene="yqhH"
                     /locus_tag="JN701_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY7365181.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            11633..>12040
                     /locus_tag="JN701_002782"
     CDS             11633..>12040
                     /locus_tag="JN701_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365182.1"
                     /translation="MRRWHTCFFILLFCGFSGNVLCKESINEAIERLYQPLTSNDDIP
                     ITDEFSLDLRSNMSATPGKMTPWLNNNLLCRCVDDAFVSAEIIQSTHLNGGRTHVLLQ
                     LVIKQNRTDIHRLLGLMLVRENDNHRWLIEDIDD"
BASE COUNT         3120 a         3054 c         2999 g         2867 t
ORIGIN      
        1 acggaactta agccgtcagg tgccagggcg atataccccg ccttcgtcac tcgccgtgcc
       61 acatcttcga tatacggatt cagtccacga ttctcatgca ccaccaccac ggctggcgtt
      121 ttgccgctca tttttgcggg tttcaccaga taaccccgta cctcgccgtg accatttggc
      181 gaaggatacg tgatgtactc agcaacaatc tccgggtcgg taaactctac ctgagtcgtc
      241 agcacataat ttggcttgag caaatcaaac aacgctaatg ccgtcatccc gcccaccgca
      301 tacttcgccg caaggttgag gaactcacgt ttcgaaattt tcccgtgagc gtaatagtcg
      361 tagtaatcca acaactcttg tgggaaatct ttggcggtta aacgcggcat cgttacattc
      421 ctcaactggt gttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact
      481 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
      541 aattgaacgg tagaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
      601 gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcgttat cgctcggttt
      661 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc
      721 gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
      781 cgcagaagag aactttggtc gcctgctgcg ggaggatttt gccgcttatc gcgatgaact
      841 gattatctct accaaggctg gctacgatat gtggcccggc ccttacggct atggcggttc
      901 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
      961 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
     1021 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
     1081 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
     1141 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
     1201 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
     1261 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
     1321 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
     1381 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
     1441 tgatcgcgtg acgtcggtat tgattggtgt cagccgcgcg gagcaactag aggagaacgt
     1501 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
     1561 cgccgatggc gagctgaatc tgtggcaggc gtcttccgat aaatgacctg ttaataacgg
     1621 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
     1681 ccatcacaaa tgcagagagc acaaacgtca gatggataat gacgtaccac atcagtttgt
     1741 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
     1801 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccagttca
     1861 gcttctcttt gttctcggag atatccagct gcgagacgaa attctcataa ccggaaaaca
     1921 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
     1981 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
     2041 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
     2101 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
     2161 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
     2221 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
     2281 atcgagagcg tctaccctta aaagacgttg aacctgaagg gagaaaaaca atgtctcatt
     2341 taaaagaccc gactacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
     2401 caggcatcca ggcgaagatg acaccggtac cggattgcgg cgagaaaacc tatgttggta
     2461 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
     2521 ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
     2581 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
     2641 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
     2701 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
     2761 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
     2821 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt atcatcacca
     2881 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
     2941 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
     3001 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
     3061 aggataagat cccgcagttt ggtcagcaaa cgccgatgaa gcgtgcgggg caaccggcgg
     3121 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
     3181 tgcacggtgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
     3241 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca
     3301 aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc
     3361 aggcattgag cacctaatgc cggatgcggc gtgaacgcct tatccggcct acaaacgcac
     3421 gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat
     3481 tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc
     3541 ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tatccgctcg
     3601 gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt
     3661 ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata
     3721 aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac
     3781 atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc
     3841 gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag
     3901 atgcattgcc atcggatatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc
     3961 agcggcgctg gcttccagat ccaggtcacg gctttgcagc aacaatacct gcgctgcaac
     4021 atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac
     4081 cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg
     4141 cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat
     4201 gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata
     4261 gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct
     4321 ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg
     4381 cgcttcattg aacaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt
     4441 ggcctggttt aaggaacgcg cttcagccag cagttgctgc tcgcgcttta ggcgacgctt
     4501 ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta cggaggccaa
     4561 aataagccca atcatcacgc acttaacgac aatatcggcg tgctgataca taccccagac
     4621 ggaaaggtct gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa
     4681 aatctgcgca taataatatc aaaacgacgt cgaattgata gtcattctca ttactatttg
     4741 catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca
     4801 cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc
     4861 atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc
     4921 ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata
     4981 gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga
     5041 cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt
     5101 ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg
     5161 gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga
     5221 tgaccaacac tgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg
     5281 gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc
     5341 caaacactaa aatcgtgttt ctggaatcgc caggctccat caccatggaa gtccacgacg
     5401 ttccggcgat tgttgccgcc gtacgcagtg tggtgccgga tgccatcatt atgatcgaca
     5461 acacctgggc agccggtgtg ctgtttaagg cactggattt tggcatcgat gtttctattc
     5521 aagccgccac caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca
     5581 atgcccgttg ctgggagcag ctacgggaaa acgcctatct gatgggccag atggtcgatg
     5641 ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac
     5701 atcatgaaag cagtctgaaa gtggctgaat ggctggcaga acatccgcaa gtagcgcgag
     5761 ttaaccaccc tgctctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag
     5821 gtagcagcgg gctgttttcc tttgtgctta agaaaaaact cagtaatgaa gagctggcga
     5881 actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt
     5941 tgatcctggc gaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt
     6001 ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg
     6061 atctggacgc cggttttgcg cgaattgtat aacattgcca cttttggaca attttgcaga
     6121 catttaattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgaatc
     6181 aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt
     6241 attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat
     6301 attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg
     6361 cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccag
     6421 ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc
     6481 tggctaagtt atattcaggg gcgctggtta gggaatacca aaacggtgaa aggctggctg
     6541 gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg
     6601 ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg
     6661 ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg
     6721 tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc
     6781 catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc
     6841 ttgttaggca tgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc
     6901 ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc
     6961 cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc
     7021 gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat
     7081 catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac
     7141 agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc
     7201 gacgctcagg ttttcggtgt atttattctc aatccgtttc agcacgcggc taatcagact
     7261 gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac
     7321 gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc
     7381 catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt
     7441 aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag
     7501 ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt
     7561 ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc
     7621 ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat
     7681 gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcaccataca acaaacgcac
     7741 atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc
     7801 cagcaagcgg caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca
     7861 atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat
     7921 tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag
     7981 atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct
     8041 gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat
     8101 tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt
     8161 tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga
     8221 gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt
     8281 gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc
     8341 agcggataac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga
     8401 gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc
     8461 caacgcaggc gcggtgatct cccgtaaaac cactggcgac aagcaggcgt tccattctgc
     8521 ccatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg
     8581 tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa
     8641 accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctgatcga
     8701 agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg
     8761 ggcggcgacg caggcgctga acggtttgat tggcgctggc gtaccgcagg actgggcaac
     8821 gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc
     8881 tatcgtcctg cctgcactgt ggaatgaaaa acgcgatacc aagcgcgcta agctgctgca
     8941 atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc
     9001 gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta
     9061 cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac
     9121 ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc
     9181 ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc
     9241 acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat
     9301 ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg
     9361 caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt gggttatcgc
     9421 tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat
     9481 gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag
     9541 cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc
     9601 tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg
     9661 atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat
     9721 cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt
     9781 catccgctga tgcaacaacg ccagctacac gcctggaacg cgacacacaa aatccagacc
     9841 gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc
     9901 gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat
     9961 agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat
    10021 gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc
    10081 aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac atgcaaattc tcccggtggc
    10141 ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt
    10201 gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc
    10261 tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat
    10321 gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc
    10381 ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag
    10441 tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc
    10501 gcttaccctg aacgacaatc ttacgctgcc gggcaatatt ggctggctgg attatgatcc
    10561 ggtttgtgat tgtcaggatt ttggcgatct ggtgctagaa agcgttgcga taacccaaac
    10621 tgacgccgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa
    10681 gaccacgcag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg acgatattgt
    10741 cagcaatcat ggcagtgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc
    10801 tttagcttcg ttgcaaaaag aacagccgga agcctttgtt gccgaactct ttgaacatat
    10861 tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa
    10921 tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga
    10981 acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt
    11041 tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac
    11101 gctgaccaat ggcaacaacg aagagcaaga actggtttta cagcggcgcg aaggcaagtg
    11161 ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa
    11221 aactgccgcc agattaaagc aatgagctga attaaataac aattagccgg aacaataaat
    11281 aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc
    11341 ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcagc tggcgagcaa tgtgcaaaca
    11401 ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat
    11461 gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat
    11521 tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt
    11581 tgccgggtta taggctgttt gctaaaaaaa agccaaacat aaggacatga ttatgcgacg
    11641 ctggcacacc tgttttttta ttctgctgtt ctgtggcttc agcggaaacg tgttgtgcaa
    11701 agaatcgatt aatgaagcga tagaacgttt gtaccaacca ttgaccagta atgacgatat
    11761 accaataacc gatgaattca gtctcgattt gcgaagtaat atgagcgcta ctcctggcaa
    11821 aatgacaccc tggctgaata ataatctttt gtgtcgctgt gtcgatgacg ctttcgtttc
    11881 tgcagaaata atccaaagca cacacctaaa tggcggccga acgcatgtac tgttgcagct
    11941 ggttatcaaa caaaatcgca cagatatcca ccggttactg gggctaatgc tggttcgtga
    12001 aaatgataat catcgttggc taatagaaga catcgatgac
//
LOCUS       DADLTD010000129        12022 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLTD010000129 DADLTD010000000
VERSION     DADLTD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.129"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1368
                     /locus_tag="JN701_002783"
     CDS             <1..1368
                     /locus_tag="JN701_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7365183.1"
                     /translation="KQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQE
                     ARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELA
                     KLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEA
                     ELLGYGQGERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLS
                     ERLKEQEAFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNIT
                     EMLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGA
                     VANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGV
                     RAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDG
                     GMLSG"
     gene            1361..1702
                     /locus_tag="JN701_002784"
     CDS             1361..1702
                     /locus_tag="JN701_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365184.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            1702..2400
                     /locus_tag="JN701_002785"
     CDS             1702..2400
                     /locus_tag="JN701_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY7365185.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            2411..3154
                     /locus_tag="JN701_002786"
     CDS             2411..3154
                     /locus_tag="JN701_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365186.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAPPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            3052..3732
                     /locus_tag="JN701_002787"
     CDS             3052..3732
                     /locus_tag="JN701_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY7365187.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            3973..7446
                     /locus_tag="JN701_002788"
     CDS             3973..7446
                     /locus_tag="JN701_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY7365188.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTSLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            7514..8113
                     /locus_tag="JN701_002789"
     CDS             7514..8113
                     /locus_tag="JN701_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7365189.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            8178..9174
                     /locus_tag="JN701_002790"
                     /pseudo
     CDS             8178..9174
                     /locus_tag="JN701_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216474.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
     gene            9187..9585
                     /locus_tag="JN701_002791"
     CDS             9187..9585
                     /locus_tag="JN701_002791"
                     /inference="COORDINATES: protein motif:HMM:NF025253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HAY7365190.1"
                     /translation="MLTTANTWTQAQTFNGGINGNLTVTGNGSFNDIQIRSDKRNKRN
                     LVKLDNALDRLEALTGYLYEIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGEK
                     RLRLNYNGIIALLVEGFKTLRHEIKELREK"
     gene            9671..10183
                     /locus_tag="JN701_002792"
     CDS             9671..10183
                     /locus_tag="JN701_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365191.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            10259..10471
                     /locus_tag="JN701_002793"
     CDS             10259..10471
                     /locus_tag="JN701_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY7365192.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(10613..10937)
                     /locus_tag="JN701_002794"
                     /pseudo
     CDS             complement(10613..10937)
                     /locus_tag="JN701_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(11073..11321)
                     /locus_tag="JN701_002795"
     CDS             complement(11073..11321)
                     /locus_tag="JN701_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7365193.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(11676..>12022)
                     /locus_tag="JN701_002796"
     CDS             complement(11676..>12022)
                     /locus_tag="JN701_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7365194.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT         2930 a         2830 c         3684 g         2578 t
ORIGIN      
        1 aaacagcaga gtgccctgac aaaaacagag cgtgagctgg ccctgtccag actgaaaggg
       61 gaagaaaaag agcgtgtccg actggggtat gcggcggatg acctcggttt tgtgggtgat
      121 ccgtatcagg aggcgagaca acgttatatc agtaatgccc tggaagcctg gcgcaataac
      181 gaggcgaata aacccaaatc ccggggtgga aaatcagaga cggaaaaagc ggaagacagt
      241 ttttcccggc tgctgaagca gcagaaagag caactggcac tggcggggca gaatacagag
      301 ctggcgaagc tgaaatacca gactgcgcag ggcgaactga aaaccctgac ggagatgcag
      361 aagcaggaac tgctgcgcaa tgcggccctg attgaccagc aaaaaatccg ggagcagttg
      421 cggtcccggg aagagaccct gaagaatgat aatgtggctg ctcgtgcatc aaatgaagcg
      481 gaactgctgg ggtacgggca gggagagcgg ctccgtgaac gcatgcggga gttgcagcag
      541 atccgcgaca gtttccgcca gaaggatgcg gaccttcagt ctcagtatca gaccggggat
      601 atcagtgagg atttttacag acaggctctg gcacagaacg cgcagtatct gagtgaacgt
      661 ctgaaagagc aggaagcctt ttatgccgaa tcggatgtgc agcgtgcgga ctggcagaaa
      721 gggctgcagg agggattcag taactgggtg gataatgcgt ccgattacgc ctcacaggca
      781 gcacagcttg cgacggaggg tatctcaggg atggtgaata acatcacgga gatgctgaac
      841 ggaaataaag tggaatggcg cagctgggcc tcatcagtgc tgcaggaaat atcaaaagtt
      901 cttatgaatg ccgcgattgt caacgggatc aagacggcgg caaacagtat gtccggagcg
      961 ggaggattta tcggcagtat tggtagctgg ctgggcggtg cggtggccaa tgcaaaaggc
     1021 ggtgtgtata cctcggcaaa cctgagtgcg tacagcaaca gtattgtgga tacgcccacg
     1081 tactttgcct ttgcaaaagg ggcgggactg atgggggagg ccggtcctga agccattatg
     1141 cccctgaccc gggcggcgga tggctcgctg ggtgtgcgag cggtgggcag tatgaacggc
     1201 agtgcaggtc tggtgtattc cccggtctac catatcgcca ttcagaatga cgggactaat
     1261 ggccagatag ggccggaagc tgcgggcagc cttgtgcaac tgattgacca gcgggtgcag
     1321 gcggtgatgc tgtccatgcg acgtgacgga ggaatgctga gtggctgaga taaaaacgct
     1381 gcatctggtc ccgcgtgaag ggatgcaggt gagtgagaaa ccgtcggtgg cgagggtacg
     1441 gtttggtgac ggttatgaac agcgccgccc gacgggactt aatgcccgac tgaagacgtt
     1501 tcaggcggtg ttccgggtga cggatgaggc gacccggcga tggctggaag agtttttatc
     1561 gtggcatggt ggttaccgtg cctttttgtg gcgaccgccg aaacataacc ggacggtgag
     1621 ggtggtatgc cgggagtgga gcgtcacaga taacgccagg tacagtgatt tcagttgtac
     1681 gattgagcag gtggtgaact gatgcaggat attcacgaag aaagtctgag cgagtcggtt
     1741 aaatcagagc agtcaccgcg ggtggtactc tgggaaattg acctgacggt gcagggtggt
     1801 gagcggtatt ttttctgtaa tgagctgaat gaaaaagggg agccggtcac ctggcagggg
     1861 cgtaagtatg aggcataccc gattgacggc agcggctttg agatgaacgg ccggggcagc
     1921 agtgccagac cgtcgctgac ggtgtccaat ctgttcggtc tggtcaccgg gatggcggaa
     1981 gacctgcaga gtctggtggg ggccacggtg gtccgccgcc gggtgtatgc ccgttttctg
     2041 gatgcggtga atttcgttgc gggcaatccg gaggcggacc cggagcagga gctgagtgac
     2101 cgctgggtgg tggagcagat gtcgcagctg acagccatga cggcctcgtt tgtgctggct
     2161 acaccgaccg agacggacgg ggcgctgttt cccggtcgta tcatgctggc gaacacctgt
     2221 atgtggacct accgctctga tgagtgtggt tacacgggcg gggctgtggc ggatgagttc
     2281 gataaaccca ccacggatat ccgtaaggac agatgcagca agtgcatgcg cgggtgtgaa
     2341 ctgcgcagga atgtcggcaa ttttggcggt ttcctttcca ttaataaact ttcgcagtaa
     2401 atcccgattt atgacacaga ctgaatcagc gattctggcg catgcccggc ggtgtgcgcc
     2461 tgcggagtcg tgcggcttcg tgataagcac gccggagggg gagcggtata tcccttgtgt
     2521 gaatatttct gcagagccgg aggcgtattt tcgtatcgca ccggaagact ggctgcgggc
     2581 agagatgcag ggggagattg tggcactggt ccacagtcat cccggtgagc tgccctggct
     2641 gagcgaggct gaccggcggc tgcagataaa aagcgcactg ccctggtggc tggtctgccg
     2701 gggggaaatt cataaattcc gctgtgtgcc acatctgaca ggacggcgct ttgagcacgg
     2761 ggtgacggac tgttacacgc tgttccggga tgcataccat ctggcgggaa ttgatatgcc
     2821 ggattttcat cgcgaggatg actggtggcg caacggccag aacctgtacc tggacaatat
     2881 ggcggtcacc ggcttttacc gggtgcccct gtcctctgca ccgccgggcg atattctgct
     2941 gtgctgcttt ggtgcttcgg taccgaacca tgccaccatt tactgcggca acggtgagct
     3001 gcttcaccat ctgcctgaac aactgagtaa acgggagagg tattccgaaa aatggcaacg
     3061 acgaacgcat tctgtctggc gtcaccgcca ctggcacgca tctgccttca cggggatttg
     3121 caacgatttg gccgccgcct cagcctgtat gtgaacacgg cagcggaagc cattcgcgcc
     3181 ctgtcgatgc agatgccggg ctttcgccgt cagatgaacg aaggctggta ccagatacgt
     3241 attgccggtg atgacacggc accggaggcg gtgtatgccc gtcttcacga acagctgggt
     3301 gagggaacgg tcatccacat tgtgccgcga ctggccgggg ccggaaaggg tggactgcag
     3361 attgtgttgg gggcggcagc catcgtgggc tctttcttca ctgccggggc atcaatggcg
     3421 ttatggggtt cagccctggc agccggtggt ttttctgcca ccacgatgct gttttcactt
     3481 ggagccagca tgattctggg cggtgtggcc cagatgctgg ccccgaaggc aaaaacaccg
     3541 gattaccgcg caacggataa cggcagacag aacacgtact tttcctcgct ggataacatg
     3601 attgcccagg ggaacccgat gccggtgcct tacggggaaa tgctggttgg ctcccgccgt
     3661 atatcccagg acatcagcac ccgtgatgaa ggcgggggcg gaaaggtcgt ggttatcggg
     3721 cgacagggat aaaacataaa aaaaccccgc agtgatcgcg gagctgcggg gacagacaaa
     3781 tgaagatcaa tgtgaaggag ttgtttttgt tactcgggca aaaaaatact aacgcagcga
     3841 aattataagc gccacagtca gtgtgtgaaa atgtgaagat attcagaaat tttattccgt
     3901 catgacgcag gcacccggtg aggtgcctgt tgtttttgtg agtgaacaat tatcacggta
     3961 agaggtgatg taatgggcaa aggtggcggc agggcgcaca caccgcgtga ggcgaaagac
     4021 aatctcaaat ccacgcagat gatgagcgtg attgatgcga ttggtgaggg accgatagaa
     4081 ggcccggtga aaggcctgca gagtattctg gtgaacaaaa cctcgctgac ggacacggac
     4141 ggtaatcccg tgatacacgg tgtgaccgcg gtctggcgtg ccggggagca ggagcagaca
     4201 ccaccggaaa gctttgagtc ctccggctct gaaactgtac tgggtgtcga agtgaccagg
     4261 gcaaaaccgg taacacgcac cattacgtca gcgaacattg accgcctgcg ggtgaccttc
     4321 ggggtgcagt cactggtgga gaccacgtca aagggtgacc gtaatccgtc ctctgtccgt
     4381 ctgctgattc agttacagcg taacggtaac tgggtgacag aaaaggatgt caccattaac
     4441 ggcaagacca cctcacagtt ccttgcgtcg gtgattctgg ataatctgcc tccccgcccc
     4501 tttaacatcc ggatggtcag ggagacggcg gacagcacca cggaccagct gcagaacaga
     4561 acgctgtggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcc
     4621 attgtgggga tgcaggtgga tgcggagcag tttggtggtc agcagatgac ggtgaactac
     4681 catatccgcg gtcgcatcat ccaggtgccg tcaaactatg acccggaaaa acgcacgtac
     4741 agtggtatct gggacggcag tctgaaaccg gcatacagca acaacccggc ctggtgcctg
     4801 tgggacatgc tgactcaccc gcgctacggc atgggaaaat gtctgggggc ggcggatgtg
     4861 gacaagtggg cgctgtatgc catcgggcag tactgcgacc agacggtccc ggatggtttc
     4921 ggggggaccg agccgcggat gacctttaat gcgtacctgg cacaacagcg taaggcgtgg
     4981 gacgttctca gtgatttctg ctctgcgatg cgctgtatgc cggtatggaa cggtcagacg
     5041 ctgacgttcg ttcaggaccg cccgtcggat gtggtgtggc cgtacaccaa cagcgatgtg
     5101 gtggtggatg ataacggcgt gggattccgc tacagcttca gtgccctgaa ggaccggcac
     5161 acggcggtgg aggtgaatta caccgacccg cagaacggct ggcagacctc cacggaactg
     5221 gtggaagacc cggaagccat actgcgctac ggacgcaacc tgctgaagat ggatgcgttt
     5281 ggctgtacct gccgcggtca ggctcaccgt gcaggtctgt gggtgataaa gaccggactg
     5341 ctggaaacgc agacggtgga cttcacgctc gggtcacagg ggctgcgtca cacaccgggt
     5401 gacattattg aaatctgtga taacgactat gctgggacta tgaccggcgg acgtgtcctg
     5461 tccattgatg ctgccacccg caccctgacg ctggaccgtg aggttaccct gccggagaca
     5521 ggtacatcgg cggtgaacct gattaacggc agtggtaagc cggtgagtgt ggacatcacc
     5581 gcacaccccg cgccggaccg gatacaggtc agtaccctgc ctgatggtgt ggagacatac
     5641 ggggtgtggg gactctccct gccgtcactg cgccgtcgcc tgttccgctg tgtctccgtc
     5701 cgggaaaaca cggatggcac ctttgccatc acggcggtgc agcacgtacc ggaaaaagaa
     5761 gccatcgtgg ataacggtgc ccgctttgag ccgcagtcag gctccctgaa cagcgtcatc
     5821 ccaccggcag tgcagcacct gacggtggag gtgagcgcag ctgacggcca gtatctggca
     5881 caggcgaaat gggacacgcc gcgggtggtg aagggggtgc gcttcagtct gcgactgacc
     5941 agcggaagcg gagaagacag ccgtctggtg accaccgcta tcactgcgga tacagagcat
     6001 cgtttcagtg gtctgccgct cggggaatac accctgacag tcagggcaat taacagttat
     6061 ggccagcagg gcgaaccggc caccaccacc ttccggatta acgcgccagc aaaacccgcc
     6121 accattgaac tgacgccggg gtattttcag ataacagcgg taccgcgtct tgcggtgtat
     6181 gacccgacgg tacagtttga gttctggttt tcggaggcaa aaatcgcaga cacatctcag
     6241 gtggaaacct ctgcccgtta tctggggacc ggcagtcagt ggagtgtatc cggcccgcac
     6301 attaagcccg ggaaggattt ctggttttac gtgcgcagcg tcaacctggt ggggaaatct
     6361 gcttttgtgg aggccagtgg acgggccagc aatgatgcag aagggtatct ggggctgttt
     6421 cgggaaaaaa taggaaaact gcatctggct caggggctgt gggagctgat agacaacagc
     6481 cagcttgcgg atgagatggc ggagatgaag accaccatca ccgaaacccg caatgaaatc
     6541 acacagacgg tcagtaaaac gctggaggac cagagcgcca ccatacagca gatacagcgc
     6601 gtgcagaagg acacaaatga tgaccttgct gcactttaca tgctgaaggt acagaaaaca
     6661 aaaaatggca taccctatgt tgccggtatt ggagcgggga ttgaggatac tgatggccag
     6721 cccctgagca acatactgct gctggctgac cgtattgcga tgattaaccc ggaggacggc
     6781 aacaccacgc cgttatttgt ggcgcagggg aatcagttgt tcatgaacga tgtgttcctg
     6841 aagcggctgt ttgcggtgag tatcacctcg tccggcaatc ccccgacgtt ttccctgacg
     6901 ccggagggca ggctgaccgc aagaaatgct gatatcagcg gtaacgtgaa tgcgaactcc
     6961 gggacgctca acaacgtcac gattaacgag aactgccggg ttctgggaaa actgtccgcg
     7021 aaccagattg aaggcgatct cgttaaaaca gtgggtaagg cttttccgcg ggactcccgg
     7081 gcaccggagc ggtggccatc agggaccgtt accgtcagga tttatgacga tcagcctttt
     7141 gaccggcaaa ttgttattcc ggcggtggca ttcagcggcg ctaagcatga gagagagcat
     7201 actgatattt actcctcatg ccgtctgata gtgcggaaaa acggtgctga aatttataac
     7261 cgtaccgcgc tggataatac gctgatttac agttgcgtta ttgatatgcc tgccggtcac
     7321 ggtcacatga cgctggagtt ttcggtatca gcatggctgg tgaataactg gtatcccaca
     7381 gcaagtatca gcgatttgct ggttgtggtg atgaagaaag ccaccgcagg catcagtatc
     7441 agctgaattt tataacccat atacgggcgc cagaaatggc gcctttttta ttgcagaaaa
     7501 gcgagaggta attatgcgta aagtttgtgc agccattttg tccgcagcca tctgtctggc
     7561 tgtatccggt gcgcctgcat gggcgtctga acatcagtcc acactgagcg cggggtatct
     7621 tcatgcccgt acgaacgctc ccggcagcga taatctgaac gggattaacg tgaaataccg
     7681 ttatgagttt acggacgcgc tggggctgat tacgtccttc agttatgcca atgctgaaga
     7741 tgagaaaaaa acgcactaca gcgatacccg ctggcatgag gattccgttc gtaaccgctg
     7801 gttcagcgta atggcggggc cgtctgtgcg cgtgaatgaa tggttcagcg cgtatgcgat
     7861 ggcgggtgtg gcttacagcc gtgtgtcgac tttctccggg gattatctcc gcgtaactga
     7921 caacaagggg aaaacgcacg acgtgctgac cggaagtgat gacggtcgcc acagcaacac
     7981 gtctctggcg tggggggctg gcgtgcagtt taacccgacc gaatccgtgg ccattgacct
     8041 tgcttatgaa ggttccggca gtggcgactg gcgcactgac ggtttcatcg tgggtgtcgg
     8101 ctataaattc tgattagcca ggtaacacag tgtcatgaca gcccgccggt tcaggcgggc
     8161 ttttttgtgg ggtgaatatg acagtaaaga tttctggtgt actgaaagac ggcacaggaa
     8221 aaccggtaca gaactgcaca atccagctga aagcaaaacg taacagtacc acggtggtgg
     8281 tgaacacgct ggcctcagaa aatccggatg aagccgggcg ttacagtatg gacgttgagt
     8341 atggtcagta cagcgttatt ctgttggtgg agggattccc gccgtcacat gccgggacta
     8401 tcaccgtgta tgaagattct caacccggta cgctgaatga ttttctcggt gccatgactg
     8461 aggatgatgc ccgtccggag gcactgcgtc gctttgaact gatggtggaa gaggtggtgc
     8521 gtaacgcaga ggaggcgaag aagaatgccg gagaggcgga gacgtcagcg aggaatgccg
     8581 gcatatcagc cagtcaggca gaagagagcg ctgcaaatgc tgacacttca gcaggggatg
     8641 catcggagtc agcccggcag gcggcagaaa gtgcagcctc agcaaagcag tcagaggagg
     8701 cgtcctcgtc ctcggcctct gcggccgctc aaaaagccag tgagtcatta caaagtgcaa
     8761 cagatgctga gttgtcaaaa aagacggcag aaagtgcagc cggtaatgca gtcagggatg
     8821 caacgaccgc aacagaaaaa gcccgggagt cagcagaaag cgcacagtca gcggaacaaa
     8881 gcaggatagc ggcggaagag gccgtaaacc gaatccccac cgtggtggga cctcccgggc
     8941 caaaggggga accggggccc gcgggtcctc aggggccgaa gggagataaa ggagagcgtg
     9001 gcgacaccgg cccggcaggg gccaaaaggt gacaaaggcg atccgggagt ggctggacca
     9061 gcaggtccgg caggtgcgcc ggggccgaaa ggcgataaag gtgatccggg agtagcaggt
     9121 ccagcaggtc cggcggggcc acagggaccg aagggagaca caggagcccc cgggcaagga
     9181 acagaactgc ttactactgc caatacatgg actcaggcac aaacttttaa tggtggtatt
     9241 aatggcaatt tgacggtgac cggaaacggc tcatttaacg atattcagat ccgttcggat
     9301 aaacgcaaca agcgaaatct ggtaaaactg gataatgcgt tagatcgtct ggaggcactt
     9361 actggttatc tttacgagat acagtactct gccgacggtt ggcaaacgtc ggttggttta
     9421 attgctcagg atgcacaaaa agcattgcct gaactggtaa ctgaagacgc agacgttata
     9481 tctggtgaaa aacgtctgcg tcttaactac aacggcataa ttgcattgtt agtcgagggc
     9541 tttaaaacac ttcgtcatga gattaaagaa ctccgggaga agtaaacgac agctgttgta
     9601 gtttctggtt tctactgaat ttaaattgtg gggtgacact caccccacgc attcagaagg
     9661 gggagatgag atgggggtaa catcgggatg ggtaggctct tcggctaaga gcgaaacagg
     9721 tgagcagtgg atgggagccg ccggaaccaa actgggactg agtaaacctt ttatgatgag
     9781 ccaaatggtt gggcgaacta tgggctgtaa aattgcaact gagtactata aatggaaatc
     9841 ctctgacaag gttgataact ggggcgcagt tggcgctgac tggcctttag aagaaaaaag
     9901 taaaggtaca attacaaacg ccgcaagctg cggatcaggg aggctggtag gggctgtcgt
     9961 tacactttct cactttttga cgaactctac accgacagct gctgtttatt tagccggtgg
    10021 taaagcaggt aacatcaccg taaacgtagg tggtgctaca caaaccatga tttatcaggg
    10081 cgttgttagt gggttccagt attactggtc aggttctgtt agttccgctt tcgtggaggc
    10141 aatgaaaaag acgggagtac cccaggatct aaaaattagt taaatggtaa ataaataatt
    10201 ttaaaaactt cacgttctac ataccggaga cgccggatat ataggatatt gttttaagtt
    10261 gccagagaaa tttttccgga cggatgctga taataatgat gtgccaatga cggcagcatc
    10321 atggatggca ctgagtgaag cgacagagca ggcgatgttt gcgaagggag tggagattaa
    10381 tacacggcag ttgcagatga aagccgaggt tgaggcgctg acagacctga aggcgatccg
    10441 cagttatgtt gtcggatggc ctgctggctg aataaaaaaa cgggaccacg accagtcccg
    10501 gaaccatgag ttttagggta ttagtttgtt atcatagtta gcgtgctaag tatgccatgt
    10561 caggttgatt agtgaagtga tgttgttcgc atttttgcac ggcggagaat attcagattt
    10621 ttggaaatcc atatttttcc cgtgcgcgat tacatgcttc atttacgatg ctattttcgc
    10681 cggacattgc gaattccctg catgtggacg gtttttgtaa acagagcaat atgcgttttc
    10741 tccggggggg ccggcaagag cgccacatcg gggatttttc tgattggtgc cgcgcataca
    10801 gcgatgaaaa ggagatattt gctcagtgag actgaccgaa acctttccgc cagcatcgtc
    10861 agcttctgcc cagtaaaaag agacgcggaa aatgcacaac aggcaccaca cgtcatgcat
    10921 ggattcagat tgctcataat tcaccagtac agctataaat cgtaaagaga acagcggtac
    10981 atcgtatgta agaacgtacc gcggctggct ggtgaacttc cgatagtgcg agtattgaat
    11041 gatttccagc cgttaccgat tttacgtgct aattagtgaa caaaccactc gtcagcagac
    11101 tcccaggtat ctttcagagt ttcctgaaca aaagttttag ctgaatcttt atcggcggtg
    11161 cgcgtaacag aaaggccatc gttgctggta gcttttacga tcacctctac atcgtcataa
    11221 cgtttactga tgcgtcgggt taattcttcc tttaacgcat ccacagcacc ggttggcatt
    11281 ttagtcattt tttctttggc tatgcagatt tcaatacgca taaaagtccc tctacactgt
    11341 gtttgtatac agtatcattt ttaactgtat ggataaacag tattaagggt gaaggaaagt
    11401 gattttttgg ggcatgcatg gggcaaaaat atttgttgtg gggcattttg gggcatgatt
    11461 gggacacttt gttacatatg aactttgccg atttttatat gacatggaaa tacaacgctt
    11521 tgattcttct aaataaaaaa catgctcttg ggcgttcttt agtgattttt aaaaattccg
    11581 tatcacgcag ttaaagtggc gggcatactc ttcaaggctg gtgatcccca accgcaccca
    11641 tttaggatgc gaccattgcg gtagaccgat gtaaatcata acgcgtgaag gatctcttcc
    11701 acgctacgca cacgggcgat gcgcgggtag atatgattaa tgctgttatt gtgttgctcg
    11761 gcgctggcgg cactacaggc gtcttcggca atcaccagat taaagccaag ttcccaggca
    11821 ttgcgggcgg tggattcaac accgatattg gtcgagatcc cacataacac tattgtatcg
    11881 atacctcggc ggcgcagttg caactccaga tccgtaccgt aaaacgcacc ccactgacgt
    11941 ttgatgattt cgatatcact gtcagttgca cctaatgcag ctggatgttg ccaccagttt
    12001 tcgggcaaca ctttagcggg gg
//
LOCUS       DADLTD010000130        11925 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.130, whole genome shotgun sequence.
ACCESSION   DADLTD010000130 DADLTD010000000
VERSION     DADLTD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.130"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            216..1325
                     /gene="livk"
                     /locus_tag="JN701_002797"
     CDS             216..1325
                     /gene="livk"
                     /locus_tag="JN701_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY7365195.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1373..2299
                     /gene="livH"
                     /locus_tag="JN701_002798"
     CDS             1373..2299
                     /gene="livH"
                     /locus_tag="JN701_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY7365196.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2296..3573
                     /locus_tag="JN701_002799"
     CDS             2296..3573
                     /locus_tag="JN701_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY7365197.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3570..4337
                     /gene="livG"
                     /locus_tag="JN701_002800"
     CDS             3570..4337
                     /gene="livG"
                     /locus_tag="JN701_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY7365198.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4339..5052
                     /gene="livF"
                     /locus_tag="JN701_002801"
     CDS             4339..5052
                     /gene="livF"
                     /locus_tag="JN701_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY7365199.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5449..6120
                     /locus_tag="JN701_002802"
     CDS             5449..6120
                     /locus_tag="JN701_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY7365200.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENRHLPGDRRRHVRRAGSGH
                     HDADL"
     gene            6050..6253
                     /locus_tag="JN701_002803"
                     /pseudo
     CDS             6050..6253
                     /locus_tag="JN701_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
     gene            6250..7095
                     /gene="ugpE"
                     /locus_tag="JN701_002804"
     CDS             6250..7095
                     /gene="ugpE"
                     /locus_tag="JN701_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY7365201.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATTTF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            7097..8167
                     /locus_tag="JN701_002805"
     CDS             7097..8167
                     /locus_tag="JN701_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HAY7365202.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLCVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            8164..8907
                     /gene="ugpQ"
                     /locus_tag="JN701_002806"
     CDS             8164..8907
                     /gene="ugpQ"
                     /locus_tag="JN701_002806"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7365203.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVVGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(8894..9334)
                     /locus_tag="JN701_002807"
     CDS             complement(8894..9334)
                     /locus_tag="JN701_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY7365204.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            9454..11187
                     /gene="ggt"
                     /locus_tag="JN701_002808"
     CDS             9454..11187
                     /gene="ggt"
                     /locus_tag="JN701_002808"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY7365205.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAQPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(11225..11509)
                     /locus_tag="JN701_002809"
                     /pseudo
     CDS             complement(11225..11509)
                     /locus_tag="JN701_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11709..11864)
                     /locus_tag="JN701_002810"
     CDS             complement(11709..11864)
                     /locus_tag="JN701_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365206.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         2706 a         2955 c         3389 g         2875 t
ORIGIN      
        1 aaaggcactt ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga
       61 ctgaaagata gggattttaa tcactattat cacaaaatac tgcgctaaac ccttaatcag
      121 acaggcaaaa acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca
      181 aagcaacaca acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca
      241 tcgcagggat gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg
      301 ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg
      361 cgcgtcaggc gattaaagac attaatgcca aagggggaat taagggcgac aagctggttg
      421 gcgtggaata tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg
      481 ttaatgacgg cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcat
      541 cagatatcta cgaagacgaa ggtattctga tgatctcacc gggagcgacc aacccggagc
      601 tgacccaacg cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc
      661 caacggcggc aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg
      721 acaaacaaca gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta
      781 acgccaacgt cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga
      841 tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa
      901 tggggcagat gctgcgccag gcccgttccg ttggcctgaa aactcagttt atggggccgg
      961 aaggtgtggg taacgcatca ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg
     1021 tcactatgcc aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga
     1081 aagcagacaa gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat
     1141 ctctggcgac tgcccttgag cgtactggca gcgatgagcc gctggcgctg gtgaaagatt
     1201 taaaagctaa cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc
     1261 ttaagggatt tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca
     1321 agtgatcatc ccaccgcccg taaaatgcgg gcgggtttag aaaggttacc ttatgtctga
     1381 gcagtttttg tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc
     1441 gctgatagcc atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca
     1501 cggcgaggtt tatatgattg gcagctacgt ctcatttatg atcatcgccg cgctgatgat
     1561 gatgggcatt gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat
     1621 tgccagcgcc tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa
     1681 gcgcctgatt gcgctcatct cggcaatcgg catgtccatc ttcctgcaaa actacgttag
     1741 cctgactgaa ggttcgcgtg acgtggcgct gcccagtctg tttaacggtc agtgggtggt
     1801 ggggcatagc gaaaacttct ctgcctctat taccaccatg caggcagtga tctggattgt
     1861 tactttcctc gccatgctgg cgctgacgat tttcattcgt tattcccgta tggggcgcgc
     1921 ctgccgtgcc tgcgcggaag atctgaaaat ggcgagcctg cttggcatca acaccgaccg
     1981 ggtgattgct ttgacctttg ttattggcgc ggcgatggcg gcagtggctg gcgtactgct
     2041 cggtcagttc tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt
     2101 taccgctgcg gtgctcggtg ggattggcag cattccaggg gcgatgattg gtgggctgat
     2161 cctggggatt gcggaggcgc tctcttctgc ctatctgagt acggaatata aggatgtggt
     2221 ttcatttgcc ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc
     2281 ggaggtagag aaagtatgaa accgatgcat attgcaatgg cgctgctctc tgccgcgatg
     2341 ttttttgtcc tggcgggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg
     2401 gtggtcgaca cggcttcaga catccgttgg cagtgggtgt ttatcggcac ggcggtggtc
     2461 tttttcttcc agcttttgcg accggctttc cagaaagggc taaaaagcgt ttccggaccg
     2521 aagtttattc tgcccgcgat tgatggctcc acggtgaagc agaaactgtt cctcgtggcg
     2581 ctgttggtgc ttgcggtggc gtggccgttt atggtgtcac gtgggacggt ggatatcgcc
     2641 actctgacca tgatctacat tatcctcggt ctcgggctga atgtggttgt tggtctttct
     2701 ggtctgctgg tgctggggta cggcggtttt tacgccatcg gcgcttacac ctttgcgctg
     2761 ctcaatcact attacggctt gggcttctgg acttgtctgc cgattgccgg gttaatggca
     2821 gcggcggcgg gcttcctgct cggctttccg gtgctgcgtc tgcgcggtga ttatctggcg
     2881 atcgttaccc tcggcttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt
     2941 accggcggcc cgaacggaat cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc
     3001 agccgtaccg cccgtgaagg cggttgggac acgttcagta atttctttgg cctgaaatac
     3061 gatccctccg atcgcgtcat cttcctgtat ctggtggcgt tgctgctggt ggtgctaagc
     3121 ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt
     3181 gaagatgaga tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgct
     3241 tttaccatca gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc
     3301 tttgtcagcc cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg
     3361 ctcggcggta tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtgtcg
     3421 cgcgagttga tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg
     3481 ctgatgatga tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg
     3541 aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc attattatct gtgaacggcc
     3601 tgatgatgcg cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc
     3661 aggagatcgt ctcgttaatc ggtcctaacg gtgccggaaa aaccacggtc tttaactgcc
     3721 tgaccggatt ctacaaaccc actggaggca ccattttact gcgcgatcag catctggaag
     3781 gtttaccggg gcagcaaatt gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc
     3841 tgttccgtga aatgacggtg attgaaaacc tgctggtggc gcagcatcag caactgaaaa
     3901 ccgggctgtt ctctggcctg ttgaaaacgc catccttccg tcgcgcccag agcgaagcgc
     3961 tcgatcgcgc cgcgacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg
     4021 cgagtaacct ggcctatggt gaccagcgcc gtcttgagat tgcccgctgc atggtgacgc
     4081 agccggagat tttaatgctc gacgaacctg cggcaggtct taacccgaaa gagacgaaag
     4141 agctggatga gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg
     4201 aacacgatat gaagctggtg atgggaattt cggaccgaat ttacgtggtc aatcagggga
     4261 cgccactggc aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct
     4321 atttaggtga ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac
     4381 tacggcaaaa tccaggcact gcatgaggtg agcctgcata tcaatcaggg cgagattgtc
     4441 acgctgattg gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat
     4501 ccgcgcgccg ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg
     4561 gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg
     4621 atgacggtgg aagagaacct ggcgatgggc ggcttttttg ccgaacgcga ccagttccag
     4681 gagcgcataa agtgggtgta tgaactgttt ccacgtctgc atgagcgccg tgttcagcgg
     4741 gcaggcacca tgtccggcgg tgagcaacaa atgctggcga ttggtcgcgc gctgatgagc
     4801 aacccgcgtt tgctgctact tgatgagcca tcgctcggtc ttgcgccgat tatcatccag
     4861 caaattttcg acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag
     4921 cagaacgcca accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat
     4981 gtggtgctct ccgataccgg cgatgcgctg ctggcgaacg aagcagtgag aagtgcgtat
     5041 ttaggcgggt aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga
     5101 caaatgtaaa attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata
     5161 ttgaatttgc acgatcttgt aggccggata aggcgtttac gccgcatccg gcatgaaacg
     5221 atgagtaatc tgtagagttt gattcagacc ttctattttt ccgcttatcc gtgccccatc
     5281 tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac cttactatct
     5341 tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt accccgcgag
     5401 cataaaacgc gtgaattcgc gcattcggta caacaagaga gataaacgat gaaaccgtta
     5461 cattatacag cttcagcact ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg
     5521 acgaccattc cgttctggca ttctatggaa ggggaactgg gtaaagaggt ggattctctg
     5581 gcccaacgtt ttaacgccga aaatccggat tacaaaattg taccgaccta taaaggcaac
     5641 tacgaacaga atttaagcgc ggggattgcc gcatttcgta ccggcaacgc tccggctatt
     5701 ttgcaggttt atgaagttgg caccgccacc atgatggcat cgaaagccat taaaccggta
     5761 tatgacgtgt ttaaagaggc ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt
     5821 tccggttact actccgacag caaaacgggc cacttactct cccagccatt caacagctcg
     5881 actcccgttc tctattacaa caaagacgcc ttcaagaaag cggggttaga cccggaacag
     5941 ccgccgaaaa cctggcagga tctggcggac tatgccgcga aactgaaagc ctccggcatg
     6001 aagtgcggct acgccagcgg ctggcagggc tggatccaac tggaaaatcg acaccttccc
     6061 ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa
     6121 gatctaccgc gaaggtttta ccggactgga tctggcttcg tctgccgcac agtcggtggt
     6181 gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt
     6241 gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc
     6301 ctcgggatcg cggtgattct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat
     6361 aaacaggccg tctatgccgc gccgatgacg ctcatccccg gcacacatct gctggaaaat
     6421 atccacaaca tctgggttaa cggggtaggt acgaatagcg cgccgttctg gcggatgttg
     6481 cttaacagct ttgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc
     6541 tcggcatttg ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt
     6601 tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc
     6661 aacctgaaga tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc
     6721 accacgtttc tgtttcgcca gttctttatg acgctgccgg atgaactggt ggaagcggcg
     6781 cggatcgacg gcgcatcgcc aatgcgcttc ttttgcgaca tcgtttttcc gctctccaaa
     6841 actaatctgg cggcgctgtt tgtgatcacc tttatttacg gctggaacca gtatttgtgg
     6901 ccgttgttga ttattaccga cgtggatctc ggcaccaccg tggcagggat caaagggatg
     6961 atcgccactg gcgaaggcac cacggaatgg aactcagtga tggcggcgat gttgttaacc
     7021 ctgatcccac cagtagtgat tgttttagtg atgcagcgtg ccttcgtgcg cggcctggtc
     7081 gatagtgaga aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg
     7141 caaaacccag gtcattaaac cgctgaccct tgatgtggct gatggcgaat ttatcgtgat
     7201 ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgt atggttgccg ggctggagcg
     7261 ggtgaccgaa ggcgatatct ggatcaacga ccagcgcgtg actgaaatgg agccgaaaga
     7321 tcgcgggatt gcgatggtgt tccagaacta cgcgctttat ccgcatatga gcgtcgaaga
     7381 aaacatggcg tgggggctta aaattcgcgg catgggcaag cagcaaattg ccgagcgcgt
     7441 taaagaagcg gcgcgcattc tggagctgga cggtctgctc aaacgtcgcc cgcgtgagct
     7501 atccggcggt cagcgtcagc gtgtagcgat gggccgcgcg attgtgcgcg atccggcggt
     7561 gttcctgttt gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc agatgcgtct
     7621 tgaactgcaa cagttgcacc gtcgcctgaa aacgacttca ctctgcgtta ctcacgatca
     7681 ggttgaagcg atgacgctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca
     7741 gattggcaca ccagttgaag tctacgaaaa gcccgccagc ctgtttgtag cgagttttat
     7801 cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat aacgaaggca cgcatttcga
     7861 actggacggc ggtattgagc tgccgctaaa cggtggctac cgtcagtatg ccgggcgtaa
     7921 aatgactctc ggcattcgcc cggaacatat tgtgctaagc tcgcaggcag aaggcggcgt
     7981 accgctggtg atggacacgc tggagatcct cggcgcagat aacctggcgc acggacgctg
     8041 gggcgagcag aagctggtgg tgcgactggc gcatcaggag cgcccgacgg caggcagcac
     8101 gctgtggctg catctggcgg aaaatcagct gcatcttttt gatggtgaaa caggacaacg
     8161 agtatgagta actggcctta tccccgcatc gtcgctcatc gtggcggcgg taagctggcc
     8221 ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat acggtcataa gatgatcgaa
     8281 tttgacgcga agttatcgaa agatggcgag atcttcctgc tccatgacga caatctcgaa
     8341 cgcaccagca acggctgggg cgtcgtgggt gaactgaact ggcaggattt actgcgcgtg
     8401 gatgcgggca gttggtacag caaagcgttt aaaggtgagc cgctgccgtt gctttcgcag
     8461 gtggcggaac gctgtcgcga acatgggatg atggcgaata tcgaaatcaa acccaccact
     8521 ggcactggac cattaacggg caaaatggtg gcgctggcgg cacgccaact gtgggccggt
     8581 atgacgccgc cgctgctgtc atcgtttgag attgatgctt tagaagctgc acagctggcg
     8641 gcaccggaac tgccgcgtgg tttattgctt gatgagtggc gcgacgactg gcgcgaactg
     8701 accgcgcggc tgggctgcgt ctctattcat ctcaatcata agttactcga taaagcgcga
     8761 gtgatgcagt tgaaagacgc cggattacgg attctggttt ataccgtcaa taaaccccag
     8821 cgcgcggcag agttgctgcg ctggggcgtg gattgcatct gtaccgatgc gattgacgtg
     8881 attggtccga actttacggc ccaatagttt tcaacggaat gtcaggctgc ggcgtgctgg
     8941 gctgattcag catgtcgccg tttctcctct ccggcagcat atgctgctga ttcaacgaac
     9001 tatccggatt acggttgctg tttaacatcc cgccgttggt gttgggcagc atttgctggc
     9061 gggcgggatt tcgctccccc ggctgtgact gcaacacccg ctgagaatta ttgtttatct
     9121 ggttttctaa atgctgctgt tgcaactgag tttgcgtttt cagttgctga ttcagcatcc
     9181 ctttttgctg gatttgctga gtttgcatct gggtttgcat ccgctgctgg ctgggtatct
     9241 gataccccgg ctggttaggg ttgttcagag tattaatggg ctgtgcaaag ccgacaaacg
     9301 gcaggagtgc cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct
     9361 ttaagtttac tcgcttcccg acaaaacgat gattaattca gagttatata ccaggcttag
     9421 ctggggttgc cccttaatct ctggagaata acgatgataa aaccgacgtt tttacgccgg
     9481 gtagccattg ctgctctgct ctcaggaagt tgttttagcg ccgtcgccgc gcctcctgtt
     9541 tcttacggcg tggaggaaga tatcttccat ccggtacgcg cgaaacaggg gatggtggcg
     9601 tctgtcgatg ccactgccac ccaagtgggg gtagatattc taaaggaggg cgggaatgcc
     9661 gttgatgccg ccgtggcggt gggctacgcg ctggcggtaa cgcatccgca ggcagggaat
     9721 ctgggcggtg gtggttttat gctgatccgc tcgaaaaatg gcaataccac cgctatcgat
     9781 ttccgcgaga tggcacccgc taaagcgacc cgcgatatgt tccttgatga ccagggcaac
     9841 ccggacagca aaaaatcact cacttcgcat ctggcttccg gcacgcccgg tacggtcgct
     9901 ggtttctcgc tggcgttgga taagtacggc actatgccgt tgaacaaagt ggtgcagccc
     9961 gcgttcaaac tggcacgcga tggttttatc gttaacgatg ctttggcaga cgatctcaaa
    10021 acctacggta gcgaagtgtt gccgaatcac gaaaacagca aagctatctt ctggaaagag
    10081 ggcgaaccgc tgaaaaaggg cgacaagctg gtgcaggcga acctggcaaa gagcctggag
    10141 atgattgccg aaaacggccc ggacgaattc tataaaggca cgatagcgga acagattgcc
    10201 caggagatgc agaaaaacgg tggcttgatc actaaagaag atttagcggc gtataaagcg
    10261 gtcgaacgca caccgataag tggcgattat cgcgggtatc aggtttactc catgccaccg
    10321 ccatcctccg gcgggatcca tatcgtacaa atcctcaata ttctggaaaa cttcgatatg
    10381 aagaaatacg gctttggcag cgccgatgcg atgcaaatca tggcagaagc ggagaaatac
    10441 gcctacgccg accgctcgga atatcttggc gacccggatt ttgtcaaagt accgtggcag
    10501 gcgctgacca ataaagccta tgccaaatct attgccgatc aaattgatat caataaagcg
    10561 cagccatcca gcgaaattcg ccccggcaag cttgcgcctt atgagagtaa tcaaactacc
    10621 cattactcag tggtggataa agatggtaac gcggtggcgg tgacctatac gctgaacacc
    10681 accttcggta cgggcattgt cgcgggcgag agcggtattc tgcttaataa tcagatggat
    10741 gatttctccg ctaaaccggg cgtaccgaac gtttacgggc tggtgggcgg tgatgccaac
    10801 gccgtcgggc cgaataaacg tccgctgtcg tcaatgtcac caacgattgt ggtgaaagac
    10861 ggtaaaacct ggctggtcac cggtagccct ggcgggagcc ggatcatcac tacagtgctg
    10921 caaatggtgg tgaatagcat cgattatggc atgaacgtcg ccgaagcgac caatgcgccg
    10981 cgtttccacc atcagtggtt gccggacgag ctgcgtgtcg aaaaagggtt tagcccggat
    11041 acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaaagaggc gatgggcagt
    11101 acacaaagca ttatggttgg gccggacggt gagttgtacg gcgcatccga cccgcgctcg
    11161 gtggatgatt taacggcggg gtactaaggt tagcggccct cttcgtggga agagggctat
    11221 tttgtcaggg caagccgaag gtagcctttt ttatttcgta atcctgtaga tattcttcca
    11281 gcggctttgc cgttcccaga gaatgaattt caccactgtc tttatcaata ataaaaggtg
    11341 cgttaccagc taagcgcgcg gcctcatctc cagtttcgag aaattctcgt gcttcgaaac
    11401 agaaatacca gccctagcta aagcgtccat gtagagtaat gacgaccggg agatctgcat
    11461 catcaaggta atggttcgct ttcgcgaatg cgtcgtgata agtaatcata attataataa
    11521 atattattgt tgagtgttat attattattc ctgtgtgata cattgagcaa agacgcgttc
    11581 atcttcgtca aagatattat taaatgctgg ttttgaaaaa agttcatcgg ataagatata
    11641 gtcacgaaat aagtatctgc taatatttta tgatttcttt ttcagaacgt cgaacgggat
    11701 tttcttattt aagatatcag aatcttatga ttcgggtaaa attctaaata aatcaacatg
    11761 tcattaaaat catcatagcc attagctatt tcggctaaaa tagagactac atgtcttcgg
    11821 tccatctcac ttaaggagtg tagttccgtt gtaagttttt ccatagcttg cactgctaaa
    11881 tttcgaacaa ggaattttct gctggtaatc tctaaaaaga tggca
//
LOCUS       DADLTD010000131        11901 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLTD010000131 DADLTD010000000
VERSION     DADLTD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.131"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JN701_002811"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JN701_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7365207.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JN701_002812"
     CDS             2219..2767
                     /locus_tag="JN701_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY7365208.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JN701_002813"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JN701_002813"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY7365209.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /locus_tag="JN701_002814"
     CDS             complement(3663..4853)
                     /locus_tag="JN701_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY7365210.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="JN701_002815"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="JN701_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY7365211.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JN701_002816"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JN701_002816"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY7365212.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JN701_002817"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JN701_002817"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY7365213.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..>11901
                     /gene="pepN"
                     /locus_tag="JN701_002818"
     CDS             9765..>11901
                     /gene="pepN"
                     /locus_tag="JN701_002818"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY7365214.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTD"
BASE COUNT         3039 a         2970 c         3008 g         2884 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgtgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagcaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata ggaaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccatgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttatc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccttggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctgcaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa gcggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaata cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag cctccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac tttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gacgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggata cctatgttac ctggcatctg caatcagaca
     8221 agcagaattc gcaaatgcag cgaaagattt cggaattaaa ggtaaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc tttttataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactggc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatatt
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc taggtccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgcgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaat tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcatcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaggg cgcactggtc atcagtaatt
    10021 tgccggagca ttttacgctt aagatcgtta atgaaataag cccggcggcg aataccgcgc
    10081 tggaagggct ttatcagtca ggcgatgcgc tttgcaccca gtgtgaagcc gaaggtttcc
    10141 gccatattac gtattatctc gaccgcccgg acgtgctggc gcgttttacc accaaaatta
    10201 ttgccgataa aaccaaatat cccttcctgc tttccaacgg taaccgcgtt gcgcaaggtg
    10261 aactggaaaa cggacgccat tgggtacagt ggcaggaccc gttcccgaaa ccgtgctacc
    10321 tgtttgcgct ggtggcaggc gactttgatg tactgcgcga cacctttacc acgcgttctg
    10381 gtcgcgaagt ggcgctggag ctgtacgtcg atcgcggcaa ccttgatcgc gcgccgtggg
    10441 cgatgacctc gctgaaaaac tcaatgaaat gggatgaaga acgcttcggc ctggagtatg
    10501 acctcgacat ctatatgatc gtcgcggtgg atttcttcaa tatgggcgca atggagaata
    10561 agggtttgaa tatctttaac tccaaatatg tgctggcccg caccgacacc gccactgaca
    10621 aagattacct cgatattgaa cgcgttatcg gccatgaata tttccataac tggaccggta
    10681 accgagtcac ctgccgcgac tggttccagc tcagcctgaa agaaggttta accgtcttcc
    10741 gcgatcagga attcagctct gaccttggtt cccgtgcagt taaccgcatc aataatgtgc
    10801 gcaccatgcg cggattgcag tttgcggaag acgccagccc gatggcgcac ccgatccgcc
    10861 cggacatggt cattgagatg aacaacttct ataccctgac cgtttacgag aagggcgcgg
    10921 aagtgatccg tatgatccac accctgctgg gcgaagaaaa cttccagaaa gggatgcaac
    10981 tctatttcga gcgccatgat ggcagcgcgg caacctgcga cgactttgtg caggcgatgg
    11041 aagatgcgtc gaatgtcgat ctctcccatt tccgccgttg gtacagccag tccggcacgc
    11101 cgattgtgac cgtcaaagac gactacaatc cggaaaccga gcagtacacc ctgaccatca
    11161 gccagcgcac gccagccacg ccggatcagg cagaaaaaca gccgctgcat attccgtttg
    11221 ccatcgaatt gtatgacaac gaaggcaaag tgatctcgtt gcagaaaggc ggtcatccgg
    11281 tgaattccgt gctgaacgtc acccaggcgg aacagacctt tgtttttgat aatgtctact
    11341 tccagccggt gcctgcgctg ctgtgcgaat tctctgcgcc agtgaaactg gaatataagt
    11401 ggagcgatca gcaactgacc ttcctgatgc gtcatgcgcg taatgatttc tcccgctggg
    11461 atgcggcaca aagcctgctg gcaacctaca tcaagctgaa cgtcgcccga catcagcaag
    11521 ggcagccgct gtctctgccg gtacatgtgg ctgacgcttt ccgcgcagtg ctgctcgatg
    11581 agaagattga tccggcgctg gcggcagaaa tcctgacgct gccttctgtc aatgaaatgg
    11641 ctgaactgtt cgatatcatc gacccgattg ctattgccga agtacgcgaa gcactcactc
    11701 gtactctggc gactgaactg gcggatgagc tgctggctat ttacaacgcg aattaccaga
    11761 gcgagtaccg tgttgagcat gaagatattg cgaaacgcac tctgcgtaat gcctgcctgc
    11821 gcttccttgc ttttggtgaa acgcatctgg ctgacgtgct ggtgagcaag cagttccacg
    11881 aagcgaacaa tatgaccgat g
//
LOCUS       DADLTD010000132        11883 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLTD010000132 DADLTD010000000
VERSION     DADLTD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.132"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(39..353)
                     /locus_tag="JN701_002819"
                     /pseudo
     CDS             complement(39..353)
                     /locus_tag="JN701_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(468..875)
                     /locus_tag="JN701_002820"
     CDS             complement(468..875)
                     /locus_tag="JN701_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY7365215.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="JN701_002821"
     CDS             complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="JN701_002821"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY7365216.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3549..4622)
                     /gene="recF"
                     /locus_tag="JN701_002822"
     CDS             complement(3549..4622)
                     /gene="recF"
                     /locus_tag="JN701_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY7365217.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="JN701_002823"
     CDS             complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="JN701_002823"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY7365218.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="JN701_002824"
     CDS             complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="JN701_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY7365219.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7427..7507
                     /locus_tag="JN701_002825"
     CDS             7427..7507
                     /locus_tag="JN701_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365220.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7737..7877
                     /gene="rpmH"
                     /locus_tag="JN701_002826"
     CDS             7737..7877
                     /gene="rpmH"
                     /locus_tag="JN701_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY7365221.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7894..8253
                     /gene="rnpA"
                     /locus_tag="JN701_002827"
     CDS             7894..8253
                     /gene="rnpA"
                     /locus_tag="JN701_002827"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY7365222.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8217..8474
                     /gene="yidD"
                     /locus_tag="JN701_002828"
     CDS             8217..8474
                     /gene="yidD"
                     /locus_tag="JN701_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY7365223.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8477..10123
                     /gene="yidC"
                     /locus_tag="JN701_002829"
     CDS             8477..10123
                     /gene="yidC"
                     /locus_tag="JN701_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY7365224.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10229..11593
                     /gene="mnmE"
                     /locus_tag="JN701_002830"
     CDS             10229..11593
                     /gene="mnmE"
                     /locus_tag="JN701_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY7365225.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11836..>11883
                     /gene="tnaC"
                     /locus_tag="JN701_002831"
     CDS             11836..>11883
                     /gene="tnaC"
                     /locus_tag="JN701_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY7365226.1"
                     /translation="MNILHICVTSKWFNID"
BASE COUNT         2717 a         3254 c         3070 g         2842 t
ORIGIN      
        1 ggacaggggt atcgcttagt ttttcaattt cctatacgtg aaccgacttc gcgagagagt
       61 ttttccagga aacgatagtc gtcataactg agagcagttt gcgccacggt gctaccatca
      121 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
      181 tgcagctctt tcaggtagtt gtgaacgcct gcataagggc gacccgtcgt tagcacgaca
      241 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
      301 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
      361 ccaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
      421 ccgagggaaa agggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt
      481 ttccctttca gcagttccat acctgcggaa agcagatcgt tattggcttg tgaagacact
      541 tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt gttctgccag taaactggaa
      601 gccgtactgg tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgggc
      661 gccgattcca gttgctcgcc gctaactgat tgattgctct gctgattact caaccaggtt
      721 gagagaatgg cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccgccc
      781 tgctcctcaa cccaacttaa aatagcctga tatttccccg catcgccttt cagaaaggca
      841 ccgacaactt catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca
      901 aaaagtatag atctgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga
      961 tctccgccct ttttttatag cttcttgccg gatgcggcgt taacgtctta cctggcctac
     1021 aaaatcatta aaattcaata cattgcaaga ttttcgtagg cctgataagc gtagcgcatc
     1081 aggcacgctc gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct
     1141 cttcaataaa cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaacaggt
     1201 cagcggcaat cgcatcctta acggtaacgc gcagcatacg acgactttcc gggtccatgg
     1261 tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga
     1321 gaccgcgacg agactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct
     1381 gacgacgctc gccacgttcg ataaatgcat cttcttccag caagccacgc agtttctcac
     1441 ccagcgtgca gatacgacga tattcgccac cagtgataaa ttcgtgatcc agcggatagt
     1501 cagtatccac accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat
     1561 tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga
     1621 ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct tccgtcagcg
     1681 tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga
     1741 tcattttctg cgtcgcgttg tactcagaca ccagtttctc taacgcttcg ccagccaatg
     1801 ccggtgcgct ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact
     1861 gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca
     1921 gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga
     1981 agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga
     2041 tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa
     2101 gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct
     2161 caacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct
     2221 gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat
     2281 cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc gcacctttac
     2341 ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt
     2401 tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc agcagttcgt
     2461 tcatctgctg ttcaaccgcc gatttcacct cagaagaaac cagtttgtct ttggtctggg
     2521 aggagaattt cgggtccggc acttttacgg aaacgaccgc aatcaggcct tcacgcgcat
     2581 cgtcaccggt ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcgt
     2641 tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg
     2701 gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcaacgcca
     2761 cttcgacgcc aataccgtct ttttcggtgg agaagtagaa gatattcgga tggatcggcg
     2821 ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag tggaagtggt
     2881 cttctttgcc gtcacgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca
     2941 actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt
     3001 cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca
     3061 gcggagcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgga
     3121 taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac
     3181 cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga
     3241 tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt
     3301 catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac
     3361 ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg
     3421 tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt
     3481 tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct cgctcattta
     3541 tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc
     3601 gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct
     3661 tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct
     3721 atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg cgccagacgt
     3781 aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg
     3841 tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga
     3901 tcgcgttcaa aattacgttc cagcacctca gcatattctg tctctttctc ccagccgcgc
     3961 tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaagtatc ggccatatca
     4021 gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccac cagcgggatc
     4081 agctctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag cgccgcattg
     4141 cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg ttcgttgtga
     4201 aagcatgccc agtcgagaaa tgctcttctg tatttggggc cgccgttgag taaagtaaac
     4261 ccttctggcg ttatcagctg catcggcatc aggtgcgcca gctccgcgac tttatgcccg
     4321 tcagtaccgt cgatgcggac tttgctgtcg ccttgtttgt ctttggttaa gccaatcgcc
     4381 gtctcgcgct cttcgccctg taatcgccca tggagaacaa atgcctcctg ctcatggcga
     4441 atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct
     4501 tccagcacac tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat
     4561 aaggcgagat ccgcggtttc gatattgcgg aaatcgcgga tcaacaagcg ggtgagggac
     4621 attacagtct cattggcatg acaacataag ccgcgctctg gctggccgca tcttcaatct
     4681 gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca
     4741 gaacatccag cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa
     4801 cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcagtgatt ttcagctggt
     4861 tttcgctgac ataaagacgt acgccgcgga atttctcgtt agagagaatt gccgcacggg
     4921 caaacgcctg cttgagcaga tcgcagccag cttccagatg tttgtccggg ttcttcggta
     4981 gaacgcggcg gtaatccggg aagcgaccat ccaccagttt ggaggtgaag ataaagtcgc
     5041 caacgtgggc acgaatgtta ttgctgccaa tctgcacgcg cagcggattg tcgccgccgt
     5101 cgagcatacg catcagttca atcacgcctt tacgcggcac gatcaccgaa tggcttggca
     5161 aagattgacc aattggcatt gaacagactg ccagacggtg accatcggtc gctacggtgc
     5221 gcagttcttc gccttcggtt tcaaacagca taccgtttaa gtaatagcga acatcctgat
     5281 gcgccatcga aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa
     5341 attcgacttc gctctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca
     5401 gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc accttccagc tgcacggcaa
     5461 tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg
     5521 ttgctcctgg ctcgtgtggc tgaaccagcg caacacgtgc caccatttcc atctcgagat
     5581 cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg
     5641 gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct
     5701 cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa
     5761 tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc
     5821 accgtcgtgt ggtcacgacc accaaacgca tcgccaatct ccggcagact gtggttagtc
     5881 agctctttcg ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc
     5941 ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg
     6001 tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag
     6061 tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt cagcgcccct
     6121 tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcgcccggc
     6181 aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct
     6241 ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg
     6301 ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc
     6361 agggcgttga aggtatggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga
     6421 atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg
     6481 ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact
     6541 ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagcag gtgagtttta
     6601 cccagacccg tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc
     6661 acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac
     6721 gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc
     6781 caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg
     6841 gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact
     6901 tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta
     6961 ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc
     7021 gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt
     7081 aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc
     7141 actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg
     7201 aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag
     7261 atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt
     7321 tctcacagat tttcccgatt tatccacagg actttccaga actcgctaag tgtaaacgat
     7381 cctgccgcga ggcgggcacg atttacgccg catattgaaa aatttaatga ccatagacaa
     7441 aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc
     7501 cgtataatcc tccacccggc gcgccatgct ggtttccact ggtgtgaggt cgtacatttt
     7561 ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggagtgt
     7621 acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt
     7681 ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga
     7741 aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta
     7801 tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc
     7861 tgaccgtttc taagtaataa agctaacccc tgagtggtta agctcgcatt tcccagggag
     7921 ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc
     7981 acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt
     8041 acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg
     8101 cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg
     8161 aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg
     8221 cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc
     8281 tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa
     8341 ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat
     8401 taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata
     8461 ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt
     8521 gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca
     8581 gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag
     8641 tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg
     8701 tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca
     8761 gccgttccag ctgctggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac
     8821 cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa
     8881 agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga
     8941 cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa
     9001 cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcca cctttggtca
     9061 gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact
     9121 gcacaccttc cgcggcgcgg cgtactccac gcctgacgag aagtacgaga aatacaagtt
     9181 cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcaat
     9241 gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta
     9301 taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc cggtactggt
     9361 tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga
     9421 caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt
     9481 catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg
     9541 cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaaac
     9601 gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga
     9661 gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt acaaagctga
     9721 gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc
     9781 gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat
     9841 ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat
     9901 gttcttcatt cagaagatgt cgccgaccac agtgaccgac ccgatgcagc agaagatcat
     9961 gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtgct
    10021 gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct
    10081 ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct
    10141 aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc
    10201 atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc
    10261 cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga
    10321 agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt
    10381 taaagacgcc gacggcagcg tgctcgatca ggggattgcg ctatggttcc ctggcccgaa
    10441 ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga
    10501 cctgctgtta aaacgcattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt
    10561 ttccgaacgc gcgtttctta acgataaact tgacttagcc caggccgagg cgattgccga
    10621 tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc
    10681 attctccgca cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga
    10741 agcggcaatt gatttccccg atgaagagat cgatttcctc tccgacggaa aaattgaagc
    10801 ccagctcaat gacgttattg ccgatcttga tgcagtgcgt gctgaagcac gtcagggtag
    10861 tttgttgcgc gaagggatga aagtggtgat tgccggacgt cctaacgccg gtaaatcgag
    10921 cctgttaaac gcgctggcgg ggcgtgaagc ggcaatcgta accgatatcg ccggaactac
    10981 gcgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgatac
    11041 cgccgggcta cgtgaagcca gtgacgaagt agaacgtatt ggtatcgagc gcgcgtggca
    11101 ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa cagacgccgt
    11161 ggatccggca gagatctggc cggaatttat tgcccgtctg ccagcgaaac tgccgatcac
    11221 cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg
    11281 tcacgcgtta attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca
    11341 tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg cgcgtcgtcg
    11401 ccacctacag gcgctggaac aggcagcgga acatctacaa cagggcaaag cgcaactgtt
    11461 gggagcctgg gcaggtgaac tgctggcgga agagttgcgt ctggcacagc agaacttaag
    11521 cgaaatcacc ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg
    11581 tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc
    11641 attgttagca ctcctgttat tcctcaaccc tttttttaaa cattaaaatt cttacgtaat
    11701 ttataatctt aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat
    11761 ttaaacaatt ttagaataga caaaattctt gagtattata ctgtagcctc gtgtctttcg
    11821 aggaataagt gcattatgaa tatcttacat atatgtgtga cctcaaaatg gttcaatatc
    11881 gac
//
LOCUS       DADLTD010000133        11845 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLTD010000133 DADLTD010000000
VERSION     DADLTD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.133"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..620
                     /gene="cobS"
                     /locus_tag="JN701_002832"
     CDS             <1..620
                     /gene="cobS"
                     /locus_tag="JN701_002832"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY7365227.1"
                     /translation="LLGAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLAD
                     TCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAA
                     ACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGV
                     AAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            632..1711
                     /gene="cobT"
                     /locus_tag="JN701_002833"
     CDS             632..1711
                     /gene="cobT"
                     /locus_tag="JN701_002833"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7365228.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            1773..2708
                     /gene="ldtA"
                     /locus_tag="JN701_002834"
     CDS             1773..2708
                     /gene="ldtA"
                     /locus_tag="JN701_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7365229.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTSPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(2764..2839)
                     /locus_tag="JN701_002835"
     tRNA            complement(2764..2839)
                     /locus_tag="JN701_002835"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2804..2806),aa:Asn,seq:gtt)
     gene            3166..3537
                     /locus_tag="JN701_002836"
                     /pseudo
     CDS             3166..3537
                     /locus_tag="JN701_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3536..3970
                     /locus_tag="JN701_002837"
                     /pseudo
     CDS             3536..3970
                     /locus_tag="JN701_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            4313..5029
                     /locus_tag="JN701_002838"
     CDS             4313..5029
                     /locus_tag="JN701_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7365230.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGSIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(5485..5560)
                     /locus_tag="JN701_002839"
     tRNA            complement(5485..5560)
                     /locus_tag="JN701_002839"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5525..5527),aa:Asn,seq:gtt)
     gene            complement(5661..7304)
                     /gene="yeeO"
                     /locus_tag="JN701_002840"
     CDS             complement(5661..7304)
                     /gene="yeeO"
                     /locus_tag="JN701_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAY7365231.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            7309..7384
                     /locus_tag="JN701_002841"
     tRNA            7309..7384
                     /locus_tag="JN701_002841"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7342..7344,aa:Asn,seq:gtt)
     gene            7546..8808
                     /locus_tag="JN701_002842"
     CDS             7546..8808
                     /locus_tag="JN701_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7365232.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(9520..10404)
                     /locus_tag="JN701_002843"
     CDS             complement(9520..10404)
                     /locus_tag="JN701_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7365233.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RGVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRHVLETARAKDPGMAAALELSRLMGLRSQEVVQSAQSLR
                     TWKQSLERGEPRLTVVFGTKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPD
                     LKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGH
                     GDGRGRYVAQVYGRKDTD"
     gene            complement(11269..11586)
                     /locus_tag="JN701_002844"
     CDS             complement(11269..11586)
                     /locus_tag="JN701_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY7365234.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(11587..11844)
                     /locus_tag="JN701_002845"
     CDS             complement(11587..11844)
                     /locus_tag="JN701_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAY7365235.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
BASE COUNT         3071 a         2914 c         2898 g         2962 t
ORIGIN      
        1 tattgcttgg cgcgattagc gggctggtct tcatggtgct gcaggcatgg tgtggcgtac
       61 cactggcggc actgtttagc gtacttgtgc tggcgctgat gaccggggga ttccacctgg
      121 acggcctcgc cgatacctgt gatggcgtat tttctgcacg tagccgcgat cgcatgttgg
      181 aaatcatgcg tgatagccgt ttaggcaccc acggcggtct ggcattgatt tttgttgtac
      241 tggcaaagat tctggtatta agcgagttgg ctctgcgtgg cgaaccgatc cttgcattgc
      301 tggcggcagc atgtgcggtc agccgcggga ctgccgcatt attgatgtat cgtcatcgtt
      361 atgcccggga agaaggactt ggcaatgtgt tcattggcaa aattgatggg cgacaaacct
      421 gcgtcacact cggcttagcc gctatttttg ctgccgtatt attgccgggt atgcatggtg
      481 tggctgctat ggtggtgacg atggtggcga ttttcatcct cggtcagctt ctcaaacgta
      541 cgctgggcgg gcaaaccggc gatacgctgg gagctgctat agagcttggt gaactggtct
      601 tcctgctggc actgctataa agagacgtga cattcaaaca cttgccgatt tactgaatac
      661 gatccctgct atcgatcctg ccgctatgtc gcgtgcacaa cggcatatcg acgggttact
      721 caaacctgtt ggtagcctgg gaaggctgga ggcgcttgcc atacaactgg caggaatgcc
      781 ggggttgaat ggcgttccgc atgtgggcaa aaaagcggta ctggtcatgt gtgccgatca
      841 cggcgtctgg gaggaagggg gcgctatttc cccaaaagaa gtgacagcca ttcaggctga
      901 aaatatgacc cgcggaacaa ccggcgtgtg tgtgctggca gcacaagcgg gcgctaacgt
      961 ctacgtaatt gatgttggta ttgatactgc tgagcctatc cccgggctta tcaacatgcg
     1021 tgttgctcga ggtagtggca atattgcttc agctccggca atgagtcgcc atcaggcaga
     1081 aaagttgctt ttggacgtca tatgttatac gcgggagctg gcaaaaaacg gtgttacgct
     1141 gtttggtgta ggtgaactgg ggatggcaaa cacgacaccg gcagcggcaa tagtcagcac
     1201 aatcactggc cgggctcctg aagaagtggt tgggattggc gcaaacctgc cgacagataa
     1261 actggctaat aaaattgatg ttgtgcgccg ggcgattacg ttgaatcaac caaatccgca
     1321 ggatggcgtt gatgtcctgg caaaagtggg tggatttgat ttggtcggaa tggctggcgt
     1381 gatgttaggc gctgcgtcct gcggtttacc cgtgttgctg gatggatttc tttcttatgc
     1441 tgctgcgctc gcagcctgtc agatgtctcc tgcaatcaaa ccgtatctca ttccttctca
     1501 cctgtcggca gaaaaaggag cgcgtatagc gctgtcgcat ttggggctgg agccttatct
     1561 caatatggag atgcgtttag gtgaggggag tggcgcagct ctggcgatgc ccatcatcga
     1621 agctgcctgt gcgatatata acaacatggg cgaacttgct gccagtaata ttgttctacc
     1681 ggggaatacg acttctgatt tgaacagtta aaaacgaaaa gtctgataaa agctatactt
     1741 aacggatagc tttcgcgact taggaaaggg acatgatgcg tcgtgtaaat attttttgct
     1801 catttgctct gctttttgcc agccatacta gcctggcggt aacttcccca ttacctccag
     1861 agggtagccg tttagtgggg cagtcgtata ctgtaactgt tcctgatcac aatacccagc
     1921 cgctggagac ttttgccgca caaaacgggc aagggttaag taacatgctg gaagcgaacc
     1981 cgggtgctga tgttttcttg ccgaagtccg gctcgcaact caccattccg cagcaactga
     2041 ttttgcccgc cactgttcgt aaagggattg tggttaacgt cgctgagatg cgtctttatt
     2101 actacccacc agacagtaat actgtggaag tctttcctat tggtatcggc caggctgggc
     2161 gagaaacccc gcgtaactgg gtgactaccg ttgaacgtaa acaagaagct ccaacctgga
     2221 cgccaacgcc gaacactcgg cgcgaatatg cgaaacgagg ggagagtttg cccgcatttg
     2281 ttcctgcggg ccccgataat cccatggggc tgtacgcgat ttatattggc aggttgtatg
     2341 ccatccatgg taccaatgcc aattttggta ttgggctccg ggtaagtcag ggctgtattc
     2401 gtctgcgcaa tgacgatatc aaatatctgt ttgataatgt tcctgttggg acgcgtgtgc
     2461 aaattatcga ccagccagta aaatacacca ctgaaccaga tggctcgaac tggctggaag
     2521 ttcatgagcc gttgtcgcgc aatcgtgcag aatatgagtt tgaccgaaaa gtgccattgc
     2581 cggtaacccc atctttgcgg gcgtttatca gcgggcaaga agttgatgta aatcgcgcaa
     2641 atgctgcgtt gcaacgtcga tcgggaatgc ctgtgccaat tagttctggt tcaagacaga
     2701 tgttttaaga gcgttggtaa ttagaaagca aaaagcctgc tagaaagcag gcttttttga
     2761 atttggctcc tctgactgga ctcgaaccag tgacatacgg attaacagtc cgccgttcta
     2821 ccgactgaac tacagaggaa tcgtgtgaac ggggcgcata ttaatggcag tgcatggtga
     2881 tgtcaaaggg tgaaatgcat ttttttgttt gtttgctgtc aatttcaaca aagtggtgag
     2941 ctttcaatct tattgggtaa tgaaccatcg tggtgcatac cctcctttta tagggcaggg
     3001 gaacgcgaca gctgattaaa ggagcaaatg acgaatctat cataatctct tatttgacag
     3061 ttggttagct tgtacatcaa cgccaaaata aaactggcaa gcatcttgca atctggttgt
     3121 aagtaatggc ggcacttggg ccgattctta aaaaccggag gcaacatgaa cttcagacgc
     3181 ctgaaatact tcgtaaaaat tgtagatatt ggtagcctga cccaggctgc tgaagtattg
     3241 catatcgcac aaccagcgct cagccagcag gttgccacac tggaaggtga gttaaatcaa
     3301 caacttttga tccgtacaaa gcggggcgtt acgccaacag acgccggaaa aattctctat
     3361 acccatgcgc gggccatttt acgtcagtgt gaacaggccc aactggcggt gcataacgtt
     3421 ggtcaggcat tatcggggca agtctcgatt ggctttgcac caggaaccgc tgcgtcatcc
     3481 atcaccatgc ccttattaca ggcggttcgc gctgaatttc cggagatcgt tatctatccc
     3541 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     3601 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     3661 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     3721 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     3781 actactgtgt gtttcagata aaccgttgca tggcgagatt aaacttcccg gccaggctaa
     3841 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt ggcattcggg cgatcgattt
     3901 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     3961 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     4021 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgtgttt
     4081 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     4141 aaaaaatccc gccaacaata taagttttta taaaattaaa tataagatta tggctttaga
     4201 aaatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     4261 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     4321 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     4381 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     4441 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     4501 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     4561 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     4621 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     4681 ggcaggttct gtcagctata tgtttgacaa tactggtgtg attgtattta aaggatcaga
     4741 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     4801 agaagaaggt agcattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     4861 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     4921 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     4981 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatatattaa
     5041 agaaatctgt ctttatggca gatttcttta atctcatata atccttataa aaaatataat
     5101 gttcaactcg tcatattgct tataaaaaat cctcgttccc agagaaataa tatttattat
     5161 aattccagtt cttctttttc tgattacaga aggcaaagtg acaattacgc atagtttccc
     5221 gataaagacg cgacagctac atcccgcata aggcattttt ctctttatcc ttgtacggta
     5281 cttcatggaa cagagttttt gaccttgcga atcgtgatgt ctgttgggga ggggcaattt
     5341 gctcactgaa gcgtgagact cgattaagcg cacgaaacac agaaatcaaa aaacccgggc
     5401 acttttttac aaggtaaccg ggtaaaaata atttttattt tttaactgtt ttgagactca
     5461 tagagatgtc tcaaaactaa aatttggctc ctctgactgg acttgaacca gtgacatacg
     5521 gattaacagt ccgccgttct accgactgaa ctacagagga atcgtgagaa cgaggcgaat
     5581 attagcgatg cccacccaca atgtcaaagc ctgttattta aatttgaaat cgtttgctga
     5641 aataatctgc attttgtcgt ttattccgac acaactggct ttttttcaca cttttgcggt
     5701 tcggatcgag ggtatttcca cagccaacgt ccagtaacca ttcgccagta aaacagcacg
     5761 gcccgcacag cccagtcggc aaacattccc atccagacac caaccacacc ccagccaagc
     5821 atgatgccca gcacataacc gacgacaacc cggcaacccc acatgctcaa catagaaacc
     5881 cacatggcgt aacgggcatc gcgagcacct ttaaatccag cgggtagccc ccatgaggcg
     5941 gaccaaatag gcataaataa agcatttagc caaatcagaa tcacaacgac atgtttaacc
     6001 tgtggatcct gggtgtaaaa cgatgccata accccggcaa agggagccgt tagccaggcg
     6061 atggccgtta atccaagagt ggaaagccag aacacatgcc gcaactgaat ctctgcttgc
     6121 gctatctgcc ctacccccaa ccttcggcct gtaatgatcg tagaagcaga gccgagcgca
     6181 cttccgggta agttgataag agccgcaatt gaaaacgcga taaaatttcc ggcaataaca
     6241 ctggtcccca tcccggcaac gaacatttgg gttaataacc gaccactggt aaataacact
     6301 gattcgacac tcgcgggaat accaatcccc atgacttccc agataatgct aaaattcagc
     6361 ggtttaaaat agctctttaa cgaaatcctt agcgcaggat taaaaccaat cgccagcacc
     6421 cacaaaattg caactgcgcc aatataacga gaaatggtta aacccagccc tgccccgaca
     6481 aatcccagtc ccggccagga gaaaaggccg taaatcaata tgccgctaat aataatatta
     6541 agaatattca ggctaccgtt aatcaatagc ggtattttcg tattccctgc accacgaagt
     6601 gccccgctac caataagagt gatggcagct gctggataac tgagtaccgt cagctccaga
     6661 taagtcaacg ccagtacttt aacttctgtc gtggcatcac ccgcgacgaa atcaataatt
     6721 tgttcgccaa aatgatgaat aagcgttgcc aacagtacgg caaacaacgt catgatcacc
     6781 aatgactgcc gcgtcgccac cctcgctcgt cgtcgatccc gcttaccgag actaaatgcc
     6841 acaacgacag tagtaccaag atcgatagca gcaaaaaaag ccataatgac catattgaag
     6901 ctgtccgcca atcccacgcc ggccatcgca tcttttccca gccagctgac cagaaaagtg
     6961 ctcagaaccc ccatcaacag gacacaggca ttctccatga agataggaac agcaagcggg
     7021 gttatctcgc gccagaacaa cactttgtag ctcttgcgtt tagcgtgcca gcgagtgccg
     7081 tgaacaacct ggcgtaaagc agaggagata ttcaaagccg accttaattg cagaaagtga
     7141 aaccacattt caaataatga gggagaatca gcaaagctgc aaagattttc gccaacaaat
     7201 tgtctgcaaa tgcaacaaac tgttgataga aacggcaaac agttggggaa tttaaaaatc
     7261 gggtttgaca aaagattttt cgccgttaag atgtgcctca acagcgattc ctctgtagtt
     7321 cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga
     7381 gccaaattcc gattcttacg ctatcttatt agtccttatt tgatattgat tcaataagtt
     7441 agcgtgaaaa tctttccaga tgtgtattca gttttccctc cgcatccggt taatttggtg
     7501 gtcagatttg ggggcaggtt ggttcgattc tggagtgacc cccaaatgtc tttaaacgat
     7561 tcaaaaatcc gtaacttaaa accctcttca aaacccgtaa aactgtctga ttcgcacggt
     7621 ctgtatctgc tggtcaatca gggcggttcg cgtatctggt atctcaagta tcgcttcagt
     7681 ggtaaagagt cccgcgtcag tctgggcgca tatcctctgg tttcacttgc tgaagcaaga
     7741 caacgacgtg acgatatccg taagctactg acgcagaata ttaaccccgc ccaccagcgc
     7801 atgtctgata aagccgcagc gtcaccggaa aagtatttta aagccgtggc gcttgcatgg
     7861 cacaaaacca acaaaaaatg gtcgcctgat tatgccgcgc gcatcctcgc cagcatggaa
     7921 aatcatatct ttccggcgat tggtcatctg cctgtcacta cgctgaaaac gcagaatttc
     7981 actgcgttac tgcgggttat tgaggataaa ggttttctgg aagtcgcgtc ccgaacccgg
     8041 cagcaactct gcaacatcat gcgttacgcc gtacagcagg gattcaccga aaacaacctg
     8101 gcgcagcatc tggaaggcgt caccgcgccg ccggttaaga atcactatcc cgccctgccg
     8161 ctggagcggt tgcccgaatt gtttgagcgc attggcgact atcagcaggg acggcagctt
     8221 acgaggctgg cggtggtgtt gactctccac ctgtttatcc gttccagcga gctgcgcttt
     8281 gcccgctggg gtgagataga tttcagaaac aaaatctgga ccatccccgc cacaagggaa
     8341 gcgattgaga aagtccgttt ctctggccgt ggcgcaaaaa tgcgtactcc atatatagtg
     8401 ccgctttctc gtcaggctat cgccattctg aagcagataa aggaaatctc cggccatctg
     8461 gaactggtgt tccccggcga ccataacccg tataagccga tgagcgaaaa cactgtcaac
     8521 cgggcgttgc gtcttatggg ctatgatacc aaaactgatg tctgtgggca tggattccgg
     8581 acaatggcgt gtagcgcatt agtggaaact gaactctggt cacgggatac cgtggagcgg
     8641 cagatgagtc atcaggagcg caacagcgtt cgggcagcgt atatccaccg ggctgaacat
     8701 ctcgacgccc gtacagccat gatgcagtgg tggtcggatt atctggatgt aagtcgcgag
     8761 gggtacgttg cgccgtatat ttatgcgcgg cggcataagg cggcctgaga cagtggctat
     8821 ataatttgtt aaaaatgaaa aagcagatcc tgattgaatc cggatctgct ttttacatta
     8881 ggcaatcgct gccagacaat ctgcaccaga aacgccataa ctgcaccagt tcagtaccac
     8941 cgcttacctg cgccagtctg ccgtcgcctg tctgtgtcag gactgcgcca gatcttatcg
     9001 aataaacatc aaactgcgcc agtgttacgg ccctttcagc cctttggcct tttccggtct
     9061 ggcgctgtta cccttagccg ccccttaccc tttccggcct ttttcctttg ctggcgcaat
     9121 cgccagaaaa atcgtcaagc tgcaccagaa gcctgccaat aggtcatacc gttcagaagc
     9181 atcgttattg agaggaaacc gtacaaaaaa tttctacgta gaaatttttt cggtggataa
     9241 cgatgaataa agagtatttt ataatgtgat ggctatcaaa aatcgaccat ccaaaaataa
     9301 ttcattgatt tataatgaat aaccatgaat ttaatagtcg atgattattg tttttatcag
     9361 tattgatagg aaagtataca aaaaattttg gccccaaaat tttttgtacg aaaaccgatg
     9421 aataacgcga aattaatagg aaaccatcca aaattatttg gggcaaataa ttttgcccga
     9481 aaaccgatgg ataactcgat cttttcaaag caacggctat caatcagtat cctttcttcc
     9541 gtaaacctgc gccacatacc gcccgcgccc gtcgccgtgt cccaaatcca tcgccgtcat
     9601 tgccagcgct tctttctcac tgaatccctg cgccagataa tgacgaatgg catcctgcgc
     9661 ccacgcatag cgcaacgagt gcggggaata tgtgccagtt aacccgagac gtgacgcctg
     9721 gctgtgccag tatttcatcg cgcttttcag gtcgggttta tctatcagcc tgccgtggcg
     9781 atcttctgcc acaaccaacg cattatccag cgcttttctg atagcgacag catccagaat
     9841 aacggtttct cgcggacgcc cgcctttggt gccgaacacc acggttaaac ggggctcacc
     9901 ccgctccagc gattgcttcc acgttcttaa tgactgcgca ctttgcacca cctcctgcga
     9961 acgtaatccc atcagccttg agagttccag cgccgctgcc atccccggat cttttgcgcg
    10021 ggcggtttcc agcacatggc ggtaatgctc cggcgtgatg gcccgcttcg taccgttacg
    10081 ggatgcgcca gacaggccca gcgagcggtt attcagtcgc tcactctgcg ccagcctgtc
    10141 acgcccggcc tgtttcagaa tgcagcgcac cgccgccatc tcattttgca gggaacgctt
    10201 cgtgataccc tgcgccagtc gttcacgaac atatccctca atataccgcg ccttaagctg
    10261 ctccaccccg cggatctgca cattctgtgc cagaaccaga cgctcacaaa accgctgcac
    10321 cagcttaata cggtcgtgaa cggtcttatg gctgccgccc gcctgttgtg ccaatcgctt
    10381 catttcctgc tccagaatcc ccatacattc cccctttcat gattgccgca aactcatccc
    10441 caaaaactca acactgatac agcgttcccc ggcgcacagg ccgatagccc ctgagttttg
    10501 tcggggctgc gaacgatacc tgagcgccgg ggcggcagcc gttgaggtgt ggtggggctt
    10561 cggttgtgct ctcagagcag ccgcccgctt gcgcgggtca tcccccggat cgtccagatc
    10621 cgcctcggta tcggttcata ttctccttat gggtaaatgg cctaaaaagg cggtgtcaga
    10681 atccagccat gagctggcgc agttgagttt tctggtgcag gtcgaaaggg cttgcgtgcg
    10741 tcactttccc gctttgcgcg gttaaagtga cgcacggatc tggtgcagca tctgagattt
    10801 atcgtctggt gcagttttac ggtcacgcgg cagcaaagcc tgacggcaat gcaggccgca
    10861 gcccgtaagg taaaaggcgc tgagggtgtc agcagatgca ggttagccgg gagaattccg
    10921 gcgcggtgca gattcagacc agcaggctgg cgaagtaaaa ggatgtatcg tattaaaact
    10981 ctggcctccg tcgcgatgtt ctgcgacccg gtgtttgcag aggtctgcac cggtaaacgg
    11041 atcgaccgtc ctgataacag gcgcttcttt caggttgtga agcgttgggc gctgtctacg
    11101 acagcataga gcaaggggga gactacgcct gtgccgctgg cgcggaaagg agaaactgtt
    11161 tttacctgca aaaggatttt ttaatcactt gttgttataa aaaatgaggg cgctgtatgt
    11221 gacagcgccc tcgttcaggt tacataaaaa atatcagctc agaatagctt acagtcgagc
    11281 cagcacctca ttcacgacag catcgggtat acgttccagc cgcttaccgc tccgagctcc
    11341 catatcaatg gttctgggct ggtcgcagcg aataacgccc gttgtttttg ttcccgcccc
    11401 gtccagtgaa acggtaaaac cggcttcgcg ggcaaagttt ccgccgctgg tgacagggac
    11461 aacaacaggc aaccgggtga acgtgttaaa cgatgccttc gatacgatga gtaccggacg
    11521 ttttccactt tgttcatggc cagtggtggg atccagtgaa acgagccaga tttctcctct
    11581 gtccatctac aggatctcct tgcccaccgc aggcgcatca atccattccc gaccctcttc
    11641 gcttatttca gcgtgcggat cgcactgcgc caacagttct tcgagcgaat agcggggacg
    11701 tttttgaggt tcaataatca ggcagccatt atcaatggtc atgcccactt cgctgtccgt
    11761 cgacagccct agcgttttca gcactgcggg gggaaccgcc agcatgacgg atccgccaac
    11821 ctttttcagg cgagtggtat acata
//
LOCUS       DADLTD010000134        11826 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLTD010000134 DADLTD010000000
VERSION     DADLTD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.134"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(37..273)
                     /locus_tag="JN701_002846"
     CDS             complement(37..273)
                     /locus_tag="JN701_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY7365236.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="JN701_002847"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="JN701_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY7365237.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1916)
                     /gene="bglJ"
                     /locus_tag="JN701_002848"
                     /pseudo
     CDS             complement(1240..1916)
                     /gene="bglJ"
                     /locus_tag="JN701_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(1874..2599)
                     /locus_tag="JN701_002849"
     CDS             complement(1874..2599)
                     /locus_tag="JN701_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7365238.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3218..3988
                     /locus_tag="JN701_002850"
     CDS             3218..3988
                     /locus_tag="JN701_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY7365239.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3979..4452
                     /locus_tag="JN701_002851"
     CDS             3979..4452
                     /locus_tag="JN701_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY7365240.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="JN701_002852"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="JN701_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY7365241.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="JN701_002853"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="JN701_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY7365242.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /locus_tag="JN701_002854"
     CDS             5888..6382
                     /locus_tag="JN701_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY7365243.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="JN701_002855"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="JN701_002855"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY7365244.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9066..10088)
                     /locus_tag="JN701_002856"
     CDS             complement(9066..10088)
                     /locus_tag="JN701_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY7365245.1"
                     /translation="MSTMNVLIFQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRVQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10227..11141
                     /locus_tag="JN701_002857"
     CDS             10227..11141
                     /locus_tag="JN701_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7365246.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11356..>11826
                     /locus_tag="JN701_002858"
     CDS             11356..>11826
                     /locus_tag="JN701_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365247.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIND"
BASE COUNT         3073 a         3013 c         2765 g         2975 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aactaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaagatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca cccctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaatg
     1741 accgccgcaa atgagatccg cgacatggca tcctgaaatg actcctgact ggtataggta
     1801 tggagcttat aagggttacc cgcaaacttt ctgaataaac tctcaatacc gatactgctc
     1861 atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg ttccattctg
     1921 cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg ctatttatcc
     1981 atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg catcgcactg tttttttggg
     2041 cgctgatagt tttattactt ttcttaagca acgaaactat ctggttgatg ccccatcctt
     2101 tacctaatag ccgcagtacc ttgcgctctg aaagagtgag aatgacggag cgatagctaa
     2161 aatcatgagt ttcattcatt gccggagagg tcagcatggc gctgatacgc tctgcgtgcg
     2221 tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca gacaataacg
     2281 tcgccgtagg gcacatgagc aattctactg cctgggaata ccaggagcgt gacaccatga
     2341 aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca caaacagaac
     2401 gtggatcttc actttcaccg gctaaatcag caatgactaa tccggaacga cgtaattgta
     2461 ataaggtaag gtcctccgca gaggcgctgg atattatttc atattcaggg aagtgagtcc
     2521 tcatgacctc ttttaatcct gcttgcataa caggtatttt actgataaca attccatttt
     2581 tgcagcatcc tggcaacata tttcctccac atccactgtc taaaaatcat gatggtttat
     2641 tcaggatatt cattccttaa atgtttccag caaattaata gattaagcga ataaattatc
     2701 cttagcattc actttcatat cagcatactc ctgctgcaca acaactccac tgcatgtgtc
     2761 agaaggttag catcaaatga taacctggtg aaatagcctg gagacattta aaatatctac
     2821 attaagttgt taggaatact taatgaaaaa cagaaatcac attcatttaa tttataatta
     2881 aatgatatcc cttcccgcag aatgaaaagt cacacaacca atatttattc aatgaaatct
     2941 gatggtttta acttgttaac attaaaaaac gtgcccggtt atctattatc ctgattcgta
     3001 aaggccaaac aataaattca ttatagtgat aatattcatg tgatagtttg tttgcggcga
     3061 gagataattc gctttttatc accgagcttg tcccgataac caggattaat cagcgcaatt
     3121 ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata caaatggatt ctcattatct
     3181 gaacaatacg cagcacgtct atgacaaagg gcgagttatg caaactgagc aacagcgagc
     3241 cgtaacacgg ctttgtatcc agtgtggatt atttcttttg caacatggtg cggaaagcgc
     3301 gttggttgat gagctttcct cacgactggg tcgggcgttg gggatggaca gcgtcgaaag
     3361 ttctatctct tcgaacgcca tagtgctgac aaccattaaa gatgggcaat gcctgacatc
     3421 gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg gtgactgaag tccagcacat
     3481 tgtgattctg gcggagcacc atctgctgga ttacaaaggc gtagagaaac gatttagcca
     3541 aattcagcca ttacgttacc caagatggct ggtagcctta atggttggcc tttcttgcgc
     3601 ctgtttctgc aaactcaata aaggtggctg ggatggtgcc atcatcacct tctttgccag
     3661 tacggccgcg atgtatatcc gccagctgct ggcacaacgt catcttcatc cgcagatcaa
     3721 cttttgcctc accgctttcg ccgccaccac catttccgga ttgcttttgc aacttcccac
     3781 tttcagcaat acacccacta tcgcgatggc cgccagcgtt ctgctgctgg tgccgggatt
     3841 tccgttgatt aatgccgtcg ccgatatgtt taaaggccac atcaataccg gactggcacg
     3901 ctgggcgatc gccagtctgc tgacactggc tacctgcgtc ggcgtggtga tggcactgac
     3961 gatttggggg ctacgcggat gggtgtgatc gagtttctgt ttgcattggc gcaggatatg
     4021 atcctcgccg ccattccagc ggtcggcttt gcgatggtgt tcaacgttcc cgttcgggca
     4081 ttacgctggt gtgcgctgct tggcgcgatt ggtcatggtt cacgaatgat cttaatgacc
     4141 agagggctga atattgagtg gtcaaccttt atggcctcca tgctggtcgg caccattggt
     4201 attcaatggt cgcgctggta tctggcgcat ccgaaagtgt ttaccgtggc ggctgttatc
     4261 cctatgttcc cgggcatatc ggcttatacc gcaatgattt cggcggtaaa aatcagccag
     4321 ttaggttaca gcgaaccgtt gatgattacc ctgttaacca actttcttac agcttcatcg
     4381 attgttggtg cgttatccat cggtctttcc attcctggat tatggttgta ccgcaagcgc
     4441 cctcgcgtat aaaattgcct cgcttaaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgatt
     4861 ttatccgcct ggcggcgcta tggggtgtgg cactaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagc gaacctgaca gtcaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcattc aaaacgggtg aagaactact ggcctggcag aaagaacaag gagcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgg ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtacgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagtgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgcttc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtt cttctcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacact tggtggtttg
     6721 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccatcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg
     7741 agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctat aagatggcac aactctgggc tccggaactg gcactctcca
     8341 ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgttcaa catgttgaca
     8401 aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttata
     8461 aaggcaatgt cgatacgctg aaaattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc
     8581 cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccaaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccacgtaagc
     8881 tcgggatcgg catggtggaa attaaagtgg tggaacgtga aggataattc ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacac cttctgcggc ctacaaaaca tcattgagaa
     9001 acagaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg
     9061 ccttatcaga aagtaattac acccttaatc aactcacgat tgttaatcac atcgcgctcg
     9121 taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc agcagtgatt
     9181 tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc gttgcggcta
     9241 cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta
     9301 aacaggccga caaataccac cgtgccgccg tgacgaatca aattcacggt gttattcatc
     9361 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcacc
     9421 cgtagctgcg cgtcaaaatc ttcggctgac gggtccagta aaggtaattc cagacgcgtt
     9481 gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct
     9541 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     9601 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     9661 gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     9721 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     9781 gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact
     9841 tgcagaccat ttttaagatc agcaatattt ttacccagcc taacaatctc cccacatatt
     9901 tcatggccta aaacacgtgg ataactaaaa aatggttgat ttccacccca ggcatgaata
     9961 tcggtaccgc aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata
    10021 ggtatctcgc gttgtttcca gactaattct ttcggctgct ggaaaattaa aacattcatc
    10081 gtagacatga ttcggctctc cattttttgt tgccttagat atcaatgaaa atgaaaaatg
    10141 gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt
    10201 tttaatggac tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga
    10261 ttaaccaggt cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa
    10321 gtgcgctggc ggaaatgtac aacatcagcc gcaccacagt gcgtcatatt ctcagccatt
    10381 tacgcgaatg cggcgtcttg acgcaggttg gaaacgacta tgttattgcg cgtaaacctg
    10441 accacgatga cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg
    10501 agcaggcttt tttcacgctg ataaaccagc gccagttacg cccaggggaa actttttctg
    10561 aactgcaact ggctcgggcc gcgggtgtca gcccggtggt tgtaagagaa tatcttttaa
    10621 aattcggccg ttataatctt attcaaagcg aaaagcgcgg ccagtggagc atgaagcaat
    10681 tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa acacattcac
    10741 ttcagcactt tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc
    10801 tcgaacgtca tcggctactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac
    10861 ttgacagaga ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat
    10921 cgcttgagat catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta
    10981 aacagcgcaa tatcattgcc gtcgatgagc atatgaccat tctcagcgct ctgatttgcc
    11041 gcagtgacct cgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa
    11101 tgattcgatc cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca
    11161 aactattagc gggtgcacgg ttttatcaaa agataaatac cgggaaattc ccgcttacct
    11221 atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg tagtaaagtc ctaaaacttt
    11281 aacctgccgt taatgtcaac cttgcgtaat gcacaattac gtaaacaatt aaaattataa
    11341 atacaggagt ccggcgtgga aaaagaaaat atcaccatcg atccgcgttc ttcatttact
    11401 ccatcttcgt cggcagatat tcccgtgcca ccagatggat tagttcaacg cagtacccga
    11461 attaaacgca ttcaaaccac cgccatgttg ttattatttt ttgcggcggt aatcaattat
    11521 ctcgaccgca gttcgctgtc ggtagcaaat ttaacgattc gtgaagaatt gggattaagt
    11581 gccactgaaa tcggcgcttt gctctccgtg ttttcactcg cttacgggat tgcgcaactt
    11641 ccttgcggcc cactattgga tcgtaaaggc ccacgcctga tgctgggact ggggatgttc
    11701 ttctggtcac tgttccaggc aatgtctggc atggtgcaca actttacgca gttcgtgttg
    11761 gtgcgtatcg gtatggggat tggtgaagcg ccgatgaacc catgcggtgt aaaagtcatt
    11821 aacgac
//
LOCUS       DADLTD010000135        11795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.135, whole genome shotgun sequence.
ACCESSION   DADLTD010000135 DADLTD010000000
VERSION     DADLTD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.135"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            487..1143
                     /gene="pphA"
                     /locus_tag="JN701_002859"
     CDS             487..1143
                     /gene="pphA"
                     /locus_tag="JN701_002859"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY7365248.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1144..1419)
                     /locus_tag="JN701_002860"
     CDS             complement(1144..1419)
                     /locus_tag="JN701_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY7365249.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRQGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(1440..1676)
                     /locus_tag="JN701_002861"
     CDS             complement(1440..1676)
                     /locus_tag="JN701_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY7365250.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1794..3233)
                     /gene="rsmF"
                     /locus_tag="JN701_002862"
     CDS             complement(1794..3233)
                     /gene="rsmF"
                     /locus_tag="JN701_002862"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY7365251.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3313..5946)
                     /gene="yebT"
                     /locus_tag="JN701_002863"
     CDS             complement(3313..5946)
                     /gene="yebT"
                     /locus_tag="JN701_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY7365252.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5915..7198)
                     /gene="yebS"
                     /locus_tag="JN701_002864"
     CDS             complement(5915..7198)
                     /gene="yebS"
                     /locus_tag="JN701_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY7365253.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7328..7825
                     /locus_tag="JN701_002865"
     CDS             7328..7825
                     /locus_tag="JN701_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY7365254.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7922..8620
                     /gene="proQ"
                     /locus_tag="JN701_002866"
     CDS             7922..8620
                     /gene="proQ"
                     /locus_tag="JN701_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY7365255.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8640..10688
                     /gene="prc"
                     /locus_tag="JN701_002867"
     CDS             8640..10688
                     /gene="prc"
                     /locus_tag="JN701_002867"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY7365256.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10880..11761
                     /gene="htpX"
                     /locus_tag="JN701_002868"
     CDS             10880..11761
                     /gene="htpX"
                     /locus_tag="JN701_002868"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY7365257.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         3033 a         2907 c         3044 g         2811 t
ORIGIN      
        1 cgcaacccca aatcgtaaag gaaaccgtag cacgtcgtat gcaagaacgt gccacggctg
       61 gctgatggat gttcgatagc gcgagtttga atgaaaatca gccggagatg attttacata
      121 attgctacgg aattattcaa tacaggaatt gcttgcgtct gcatggattg acctgaaatt
      181 ttcccgaaaa tttctctaaa aaactcgaaa aaaatggtaa ctggttgaat gtattaatat
      241 gcaatggtac gtgtcaggga ttaaaagatg aacgtaaatt tattcaacgc attaatttta
      301 aagggtttta ttgtttgttg acgaaaacag gaatcgtgtt cggtctcttt ttatctgtta
      361 aaagccagaa gcatttcctt cgctgacttt atagtcaacc ataacacaca ctctactgtc
      421 tgagtccagc gttttttaac attcttgtta agattatgtg atctttagca cgggaggaaa
      481 atattgatga aacagcctgc gcccgtttat cagagaattg cgggtcatca atggcgacat
      541 atctggcttt ctggcgatat acacggttgt cttgagcagt tgcgccgcaa attatggcat
      601 tgtcgttttg atccgtggcg agatttactt atctcagtgg gagacgttat cgatcgtggg
      661 ccgcaaagtt tacgttgtct gcagttactg gaacaacatt gggtttgtgc ggtaagaggc
      721 aatcatgaac agatggcgat ggatgcgctg gcatcccagc agatgtcttt gtggttgatg
      781 aatggcggcg actggtttat tgcgctggca gataatcaac agaaacaagc gaaaacggcg
      841 ctggaaaaat gtcagcattt gccctttatt cttgaagtac acagtcgcac cggcaagcat
      901 gttattgctc atgcggatta tccagatgat gtttatgaat ggcaaaagga cgttgatttg
      961 catcaggtct tgtggagccg ctcgcgatta ggtgaacgcc aaaaagggca gggaattaca
     1021 ggtgctgatc atttctggtt tggtcataca ccgttgcgac atcgcgtgga tattggcaac
     1081 ctgcattata ttgataccgg tgctgtcttt gggggcgaac tgactcttgt gcaattgcaa
     1141 tgattaaaaa tcaccgtact cctgtgcagg tcgccagaaa ccatctataa aatcctcaat
     1201 cggaaaacaa ccgccctggc ggatccgttg atcgctcata gaataaagac actgctgttc
     1261 cgtgttgtag acatccacaa caatatcttc acaaccgcca tccaggtagc aaacaaaaag
     1321 taccagcgcg aacatttcat ccccgaagtg tggtgccgta ccgttaagtt taggagagat
     1381 tttacaacgg gggaataacc aggacaaata acccgccatg ccgacgggtt ctttttggat
     1441 caggcaagac gcataatcca ggcatcagat tgttgatcgt aacgggtacg gtaaaggaca
     1501 gagtgttcgc catttagcag ggcagggata ctggtttcag catcccaggc atcgagttgc
     1561 atacataaat cggggtcaga tttacaagga atggttaacg ttcgatcacc cggcgattca
     1621 ccgtgtaatt cgccgtcgtc gatttcaaaa gcgcctatgc gcacactggt tttcatcttc
     1681 gtgctctcca tttagctgcc ggttgtggta cgaccagtcc gatcgcacca tttttcagcc
     1741 tagttgaggc agccgaatgt gccagtcaaa aagtgcgctt tttttgtgcg ctgtcaggcg
     1801 ttaccggtaa aaagtttccc atcgcgcacc agttcacgcg gatagctgtt tttcaatcgc
     1861 gaaccaatcc gtttggctaa accaatcggt tgatgctgga aagtaaccaa cacgtcatcc
     1921 gccactggcg cggcttgcgg gtaaacatcg cgcccgcgat accactcctc cgcttcctgc
     1981 ggtgtcagtt caaaagcgtt cacattgtcg ggggtggcaa gggcaataac tgcttcatgc
     2041 tgccagcgat aacctttgtt gtgcgtttcg gcaagtttaa tccccaagcg agaaaatcgg
     2101 actttaccga tcagggcttc aatgcccacc gggaacaacc acagttcttt gtcacgctgc
     2161 cagaggcgca ggttttcatc ccagtttaag ccaacacctg cagccgcctg gcgaatttgt
     2221 ccagcttcgc gatctttcac cgggctgaac ggaaaattac cgactttgta tttgggggcg
     2281 ggtaaggcgg gaatcgcctg agttttacgc agacgagcaa cgaagaagcc ttcgcagtcg
     2341 taaatttgtg ggaaaacatg caaaaagcct tcttcggtca gcgctttgtt tgcaccaggg
     2401 aagagatcgc caagtggtaa aaactctact gcgtcggggt aagtctcttt cagccacagg
     2461 caaacggctt cgttttcttc ctggtttaag gtacaggtcg agtaaaccag cgtaccacca
     2521 ggacgtaatg catgaaaggc gctgtcgata agctcccgtt gtgtagctgc gatttcctga
     2581 ttgctttctg gtgaccagtt ttttagcgca tcgggatctt tacgcaccac gccttcgccg
     2641 gagcagggag cgtccagcaa aatggcatca aacatttctg gcactgccgc accaaacacg
     2701 cggccatcaa aatgtgtgag cgcaacatta ctgatgccac agcggctgat attggcatgt
     2761 aacactttta cccgactggc ggaaaactca ttggcaagga ttgccccttc gttattcatc
     2821 cgcgcggcaa tttgcgtcgt ttttgagcct ggcgcagcag cgacatccat cacccgctgt
     2881 ggtgcattac cgtcagcaaa caaggcggcg acgggcaaca ttgaactggc ttcctgaata
     2941 taaaacaggc cacttaaatg ctcggcggta ctacccaatg gcaatgcatc ttcattgtcg
     3001 cgttcaatcc agaaaccttc ttcacaccac ggaattggcg taagcgtcca gccataagga
     3061 gcggttaatt gcaggaaatc agcaacggag attttcagcg tattaacgcg aatgctgcgg
     3121 cgcaacgggc gctgacaggc ggcaagaaaa tcatcaaatg agagcgtcga aggcatagct
     3181 tcgcgcattt gtgtcagaaa cgcgtccggg aaataaacgg tgtgttgggc cacgagcatg
     3241 taccactggc aaaaaaacag gcgcgcagtt tagcataaac gctccggcgc aggcacgccg
     3301 gagcagtggg cattatttgg gaagcgcagt tccccattca cgccactctt tcggttcact
     3361 ttcctgcaac aggaagtgtt tgccttcctg ggctttcggt gccagtggcg tacccggagg
     3421 cgtggcgaag gcgatgccgc cacggataaa ctggttaaag gtgccggttt tcactacgcc
     3481 gcccgtcaga ccaaagtcca gactgtaacc cgatgccaac cagaagacgg aattgttacg
     3541 caccaggtgt tgatagcgtt tactgatgcg catcgcaatc atcacgcgat ctgacaatgt
     3601 ccccagcgtc attccggtaa ccgtacctac ttccagacca cggaacagca caggcgtacc
     3661 aatacctaac gaaccggctt ccggtgcttc gacaataatg cttaagccat ccaggtaacg
     3721 cgaatcagta atagtagctt cttgcaactc aaagtcacgg cgaggattac cccgacctgg
     3781 ttcgacattg atatacggct ggaggatagt atctagatgc tcaacgcccg tggctgaaat
     3841 ttgcggtgtg accaccgaga agcgcgtacc gccgcgggcg aaggtctgga catactccgg
     3901 atagagcacc gcctttgcct gtacttcatt gcgcgcggta atcagatcca gcgtctggat
     3961 ttgcccgata tcaataccga gatagcgaat tggcatcccg accgccagtt ttccggcatc
     4021 gaaagcgtga agcgtaatct gcccactaac cgcacgggcc gctgtttcgg aagcatacag
     4081 aattcgtttg tcgcctttac gctgactggc gctggcaccg ctgaggttat cgaagctaat
     4141 ggctcccttt aatgctctgg agagcgggga tgcctgtacg gtcaggccac taccattcag
     4201 ctgaactttc gccccgcctt ctgcccagaa cacgctattg ctggtcagaa ggttgcgata
     4261 ctccggctta atatgcagat cgatatcaaa cgcgttagct cgcggacgca cggtaatcac
     4321 ttcaccaact tcaaatttac ggtacagcac taccgatcct gcctgcacat ccggcagcgt
     4381 ctctgcactc aaactcacgg ttgtggtggg taaatcgcta aggctgttct ccagcgcttt
     4441 ttccagattg gcatacagtg gatagctggc tttcatctca cctttatcgc ctggcagaat
     4501 acgtatcccg ccgttaatcc attctgaggc gctggcacca agaaactcaa cgccatccag
     4561 ccccaccttc acgtcgacac ggctgttgac gacaaatttg ctatcgcctt ttaccagttc
     4621 gcgatgctga ggctcgatgg cgacggtaaa ggtaacgcct ttgctggtga gtttacgatc
     4681 aataacctgg cctacctgca cgccgtgaag aatgagcggc tgacccgtat caataccgta
     4741 actttccggc gcggtcaggg tcagcgtcag aacatcaggt tcatgcagca gtgctttttc
     4801 gcctggcaca acaacgaact ctttgcgtgg ctcgccatcg ccgggtacca actcgaaggt
     4861 tttgccagtc agcagggcgc tgagattggc atcgctaagg gataatttcg gattacgtaa
     4921 ttcgatgcgg gtattttcac gaagcagggt aacgacgctg ggatcaactg tcatttcccc
     4981 agtgacttta ccaccaggat ttaaatccag tttagttagc tgtccgactt ccagtccctg
     5041 atacattaat ggcgtcgagt cggcggttaa tccggcccca ctcggcagtt ccagtttgat
     5101 tattacgcca cgctggctat gggcgaggtc ttcatacaga ccaaaggtat cttccgcctc
     5161 ggcaggtttc gactcttctg gtgaatcgaa ggcaatcgca ccgttaacca gtgccgccag
     5221 gctttccagt ttcaccttcg cgccactgat actgacattg gcatcaacgc cggaaacgtt
     5281 ccagaaacgg ctaccttttt tcaccagatc ggtaaaacgc cgctcgatca ggacatcaat
     5341 caccacgcct tgtttgttgg gattgatggc atagtcgtag acttttccca ccgggatctt
     5401 gcggaaatag accaatgaac cgctgttcag cgaaccgaga tcgggggctt gcaggtggat
     5461 catcagatcg ccattgttca gccgatattt cggttgggta tcgagtgcga caaagtgatc
     5521 ctgctcttta cctgtacccg gcatcatgcc gatatagttc ccaccgacga gggcgtccag
     5581 cccggagaca cctgccaacg atgcttttgg cgtcaccagc cagaattgag tctcttcgcg
     5641 cagcgcatct ttcatatcgg acttgatgct gaccttgact tcaatcttac gaagatcgtc
     5701 gctgaggctg atatcctgca ctgttccgac ttcaacgccc tgataacgaa caggcgtacg
     5761 gcccgggaca ataccatccg ccgacataaa gtcgatggtg acggtattac ttcggtcctg
     5821 ataactgtcc caaatcagcc aaccggcaat cattagcgcg atgaaaggca gcagccagaa
     5881 aggtgagata cggcgtttat ttttaatctg cgcttcagtc gtcgaagcgg gcgtttcctg
     5941 actcatgtgc atcccaaagt aagcggctgt ccagccattc cacagcaaga atagtcaaaa
     6001 ttacggctgc gccaaaataa aacgcagccg gtcccatagt aaaagcgagg atctgatcgc
     6061 gattaatcag cgacatggtt aaagatatga caaacaggtc gagcatcgac cagcgtccaa
     6121 tccaggtcac catccgcagt aacagaatgc gtgtgcgtaa accttgctgg catttaaaat
     6181 gaatgctcaa cagtaaagtg aacatgacga tcactttagt aaacggtacc agaatactgg
     6241 cgataaacac gattccggcg accgcaatgt tgctacttgc cagcgacata atcccggaaa
     6301 gaatcgtatc ttcctgccgg ccaccattca gataaatgat agaaataggc aataggttgg
     6361 ctggtaacaa caaaacgatt gacgctaaca gcgccgccca gcatttttgc aaactatgac
     6421 gacgacgcag gcgtagcggg atatggcaac gcgggcagcg accacgctga tcaggatagc
     6481 cggtaaaatg gcacccaaga cagacacgaa gtttctcgtc cctacgcgta gcggggcgct
     6541 gcggataata tcgctcccac agctcctcaa cattaagatg tgacaacgtc accgtcgtta
     6601 aaatcaccaa cgccacaaaa gagaacaagc cgacacccgc ctggatatgg gcataatcct
     6661 gtacctttat agaagcaacg ccaatgccga ccaggtagat atccagcatt acccactctt
     6721 taagacgctc aagcatcagc agtaccggac gtaaattcat tcccagtcgg ttaccaaacc
     6781 ataaatatgc tatggaggac accagaatga ggggggcacc aataacgcag aaaaagacca
     6841 tcgcccccgt tatcgtatcg ccctgtttgg tcatttgcca gatgccttgc atgacgtttg
     6901 cgtcgatacg aatacctaac agccagatat gcaacagcgg ttcgccccag gcaaacggca
     6961 tcagcaacag catggtgaaa gccattgccg ccaggcgcgt tagcgaccag tcgcgcccgt
     7021 cgcgaatttt tgcctgacag cgcggacaat aggcactctg atgagaattt atctcgggca
     7081 ggctaaacag catgtcacat tgcgggcaac gttggtaatc accacgcggc agttcctcgc
     7141 cgattgacct cactgttatc ttttttgtcg gcgtaatttg tggtgtgtta agagccatga
     7201 cgtgccaaat gtaataagaa ttaacattaa tcttaaccca tgatgggaat gatcttgagc
     7261 attaacgcat ttaatgctta ttttaatagg ctggcagttc caggtaagac aaacaagtga
     7321 ttatataatg aacaaaacag aattttacgc ggatttaaat cgcgacttta acgcgctgat
     7381 ggcgggagaa accagttttc tggcaacgct tgcgaacacc agtgcgttgt tatacgagcg
     7441 tctcactgac gtaaactggg caggttttta tttgcttgag gacgatacac tggtactcgg
     7501 accatttcag ggcaaaattg cctgtgtccg gatacccgtc gggcgcgggg tgtgcggcac
     7561 tgcggttgcc cgcaatcaag tgcagcgtat cgaggatgtt cacgcgtttg acgggcatat
     7621 tgcctgtgat gcggcgagta attctgaaat tgttctgccg ctggtggtga aaaatcagat
     7681 tattggtgtt cttgacatcg atagtaccgt cttcggtcgc tttacagacg aggacgagca
     7741 aggcttacgt cagcttgtgg cacagcttga aaaagtgctt gcaacgacgg attacaaaaa
     7801 attctttgcg agcgtcgcag gataatcaac ggataacgta gcatttactg gtagcgtcat
     7861 tataatgacg cctgttcatg cctgcgcttg ttggctacgt ccgttgtaat caggaaattt
     7921 catggaaaat caacctaagt tgaatagcag taaagaagta atcgcgtttc tggccgaacg
     7981 ttttccccac tgtttcagtg cggaaggtga agcgcgtccg ctgaaaatcg gtatttttca
     8041 ggatttggtc gatcgtgttg ccggggaaat gaacctgagc aaaacgcaat tacgatccgc
     8101 tttacgtctc tacacttcga gctggcgtta tctttacggt gttaaacccg gcgcaacgcg
     8161 tgtcgatctt gacggcaacc catgcggtga gctggacgag caacatgtag agcatgctcg
     8221 caagcagctt gaagaagcga aagcgcgtgt tcaggcacag cgtgctgaac agcaagcgaa
     8281 aaaacgcgaa gctgccgcag ctgctggtga gaaagaagac gcaccgcgcc gcgaacgcaa
     8341 gccacgtccg accacgccac gccgcaaaga aggcgctgaa cgtaaacctc gtgcgcaaaa
     8401 gccggtagag aaagcgccaa aaacagtaaa agcacctcgc gaagaacagc acaccccggt
     8461 ttctgacatt tcagctctga ctgtcggaca agctctgaag gtgaaagcgg gtcaaaacgc
     8521 gatggatgcc accgtattag aaatcaccaa agacggcgtc cgcgtccagc tgaattcggg
     8581 tatgtctttg attgtgcgcg cagaacacct ggtgttctga aacggaggcc gggccaggca
     8641 tgaacatgtt ttttaggctt accgcgttag ctggcctgct tgcaatagca ggccagacct
     8701 tcgctgtaga agatatcacg cgtgctgatc aaattccggt attaaaggaa gagacgcagc
     8761 atgcgacggt aagtgagcgc gtaacgtcgc gcttcacccg ttctcattat cgccagttcg
     8821 acctcgatca ggcattttcg gccaaaatct ttgaccgcta cctgaatctg ctcgattaca
     8881 gccacaacgt gctgctggca agcgatgttg aacagttcgc gaaaaagaaa accgagttag
     8941 gcgatgaact gcgttcaggc aaactcgacg ttttctacga tctctacaat ctggcgcaaa
     9001 agcgccgttt tgagcgttac cagtacgctt tgtcggtact ggaaaagccg atggatttca
     9061 ccggcaacga cacttataac cttgaccgca gcaaagcgcc ctggccgaaa aacgaggctg
     9121 agttgaacgc gctgtgggac agtaaagtca aattcgacga gttaagcctg aagctggcag
     9181 gaaaaacgga taaagaaatt cgtgaaaccc tgacacgccg ctacaaattt gccattcgtc
     9241 gtctggcgca aaccaacagc gaagatgttt tctcgctggc aatgacggcg tttgcgcgtg
     9301 aaatcgaccc gcataccaac tatctttccc cgcgtaatac cgaacagttc aacactgaaa
     9361 tgagtttgtc gctggaaggt attggcgcag tgctgcaaat ggatgatgac tacaccgtta
     9421 tcaattcgat ggtggcaggt ggtccggcag cgaagagtaa agctatcagc gttggtgaca
     9481 aaattgtcgg tgttggtcaa acaggcaagc cgatggttga cgtgattggc tggcgtcttg
     9541 atgatgtggt tgccttaatt aaagggccga agggcagtaa agttcgtctg gaaattttac
     9601 ctgctggtaa agggaccaag acccgcactg taacattgac ccgtgaacgt attcgtctcg
     9661 aagaccgcgc ggttaaaatg tcggtgaaga ccgtcggtaa agagaaagtc ggcgtgctgg
     9721 atattccggg cttctatgtg ggtttgacag acgatgtcaa agttcaactg cagaaactgg
     9781 aaaaacagaa tgtcagcagt gtgatcatcg acctgcgtag caatggcggt ggggcgttga
     9841 ctgaagcggt atcgctctca ggtctgttta ttcctgcggg tcccattgtt caggtccgcg
     9901 ataacaacgg caaggttcgt gaagacagcg ataccgacgg gcaggtgttc tataaaggcc
     9961 cgctggtggt actggttgac cgtttcagtg cttcggcttc agaaatcttt gccgcggcaa
    10021 tgcaggatta cggtcgtgcg ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc
    10081 agcaataccg ttcattgaac cgtatttacg atcagatgtt acgtcctgaa tggccagcgc
    10141 tgggttctgt gcagtacacg atccagaaat tctatcgcgt taacggcggc agtacgcaac
    10201 gtaaaggcgt aacgccagac atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg
    10261 agaaattcga agataacgcg ctgccgtggg atagcattga tgccgcgact tatgtgaaat
    10321 caggcgattt aacggccttt gggccggagc tgctgaagga acataatgcg cgtatcgcga
    10381 aagatcctga gttccagaac atcatgaagg atatcgcgcg cttcaacgct atgaaggaca
    10441 agcgcaatat cgtttctctg aactacgctg tgcgtgagaa agagaataat gaagatgatg
    10501 cgacgcgtct ggcgcgtttg aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga
    10561 agctggatga tctaccgaaa gattaccaag agccggatcc ttatctggat gagacggtga
    10621 atatcgcact cgatctggcg aagcttgaaa aagccagacc cgcggaacaa cccgctcccg
    10681 tcaagtaata tcaatcaggc acaagaaatt gtgcctgatt ttttaacagc ggcaagatgc
    10741 cgtaaatcag atgctacaaa atgtaaagtt gtgtctttct ggtgacttac gcactatcca
    10801 gacttgaaaa tagtcgcgta acccatacga tgtgggtatc gcatattgcg ttttgttaaa
    10861 ctgaggtaaa aagaaaatta tgatgcgaat cgcgctcttc ctgctaacga acctggccgt
    10921 aatggtcgtt ttcgggctgg tactgagcct gacagggata cagtcgagca gcgttcaggg
    10981 gctgatgatc atggccttgc tgttcggttt tggtggttcc ttcgtttcgc ttctgatgtc
    11041 caaatggatg gcattacgat ctgttggcgg ggaagtgatc gagcaaccgc gtaacgaaag
    11101 ggaacgttgg ctggtcaata ctgtagcaac ccaggctcgt caggcaggga tcgctatgcc
    11161 gcaagtggct atctaccatg cgccggacat caacgctttt gcaaccggtg cgcgccgtga
    11221 tgcctctctg gttgctgtca gcaccggttt gctgcagaac atgagcccgg atgaagccga
    11281 ggcggtaatt gctcacgaaa tcagccacat cgccaatggt gatatggtca ccatgacgct
    11341 gattcagggc gtggtgaaca ccttcgttat ctttatttcc cgtattctgg cgcagcttgc
    11401 cgcgggtttt atgggcggaa atcgtgatga aggtgaagag agcaacggca acccgctgat
    11461 ctactttgcg gttgcaacgg ttctggaact ggtgtttggt attctggcga gcattatcac
    11521 catgtggttc tcgcgtcatc gtgaattcca tgctgatgcc ggttcggcaa aactggttgg
    11581 tcgcgagaaa atgattgccg cgctgcagcg cctgaaaacc agctatgaac cgcaagaagc
    11641 aaccagcatg atggctttct gcattaatgg taagtcgaaa tcgctcagtg agttgttcat
    11701 gactcacccg ccgctggata aacgaattga agctctgcgt acgggtgaat acctgaagta
    11761 atatgaagta aatactaaaa agtgcgtcct gatcg
//
LOCUS       DADLTD010000136        11784 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.136, whole genome shotgun sequence.
ACCESSION   DADLTD010000136 DADLTD010000000
VERSION     DADLTD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.136"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            29..1414
                     /locus_tag="JN701_002869"
                     /pseudo
     CDS             29..1414
                     /locus_tag="JN701_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            complement(1471..1689)
                     /gene="marB"
                     /locus_tag="JN701_002870"
     CDS             complement(1471..1689)
                     /gene="marB"
                     /locus_tag="JN701_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY7365258.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1721..2104)
                     /gene="marA"
                     /locus_tag="JN701_002871"
     CDS             complement(1721..2104)
                     /gene="marA"
                     /locus_tag="JN701_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY7365259.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2124..2558)
                     /gene="marR"
                     /locus_tag="JN701_002872"
     CDS             complement(2124..2558)
                     /gene="marR"
                     /locus_tag="JN701_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY7365260.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVEKLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2770..3435
                     /gene="marC"
                     /locus_tag="JN701_002873"
     CDS             2770..3435
                     /gene="marC"
                     /locus_tag="JN701_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY7365261.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3460..4650)
                     /gene="ydeA"
                     /locus_tag="JN701_002874"
     CDS             complement(3460..4650)
                     /gene="ydeA"
                     /locus_tag="JN701_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY7365262.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4800..5507)
                     /gene="yneK"
                     /locus_tag="JN701_002875"
     CDS             complement(4800..5507)
                     /gene="yneK"
                     /locus_tag="JN701_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7365263.1"
                     /translation="MGRVFHTGVERVLFLFLNDFIEQFPMIHLGVPIKKAHTPHIEPL
                     PPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWSNSEVHRWFVNKDPNLITAILDIT
                     DGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDFFLFFANMLLHYHQ
                     FMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNRPRSPV
                     RPPIPATAKTPDLPERH"
     gene            complement(5456..5914)
                     /gene="yneK"
                     /locus_tag="JN701_002876"
     CDS             complement(5456..5914)
                     /gene="yneK"
                     /locus_tag="JN701_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7365264.1"
                     /translation="MVTPVSISNYIFLPDDFPVRNIAPQVKEVLKNFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLNPIYLDNFRHDGEINIFALPTQSLSLWGAYFTPASSVCSFCF"
     gene            complement(5992..6873)
                     /locus_tag="JN701_002877"
     CDS             complement(5992..6873)
                     /locus_tag="JN701_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7365265.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            6974..8362
                     /gene="sad"
                     /locus_tag="JN701_002878"
     CDS             6974..8362
                     /gene="sad"
                     /locus_tag="JN701_002878"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HAY7365266.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            8426..9352
                     /gene="glsB"
                     /locus_tag="JN701_002879"
     CDS             8426..9352
                     /gene="glsB"
                     /locus_tag="JN701_002879"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY7365267.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            9352..9711
                     /locus_tag="JN701_002880"
     CDS             9352..9711
                     /locus_tag="JN701_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY7365268.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            9850..11268
                     /locus_tag="JN701_002881"
     CDS             9850..11268
                     /locus_tag="JN701_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7365269.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            11495..11734
                     /locus_tag="JN701_002882"
                     /pseudo
     CDS             11495..11734
                     /locus_tag="JN701_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
BASE COUNT         2991 a         2662 c         3026 g         3105 t
ORIGIN      
        1 atggggcggc gcggtagccg cgcatcagtt ggaaggtggc tggaatgaag gaggaaaagg
       61 catcagtatc gctgatgtga tgactgctgg cgctcacggg gtgccgcgtg aagtgacaga
      121 aggcgttatc gacgggctta attatcccaa tcatgaagca attgattttt atcatcgcta
      181 taaaacagat attcagttat ttgccgagat gggattcaaa tgctttcgaa cttccattgc
      241 ctggacacga atctttccgc aaggtgacga acaggagccg aatgaagagg gtttacaatt
      301 ttatgatgat ctgttcgatg aatgccttaa gcagggaatg gaacctgtgg tgacgctttc
      361 acattttgag atgccttatc atctggtgac aaaatatggt ggctggcgaa accgtaaact
      421 gatcgacttt tttatccgct tcgcatcaac ggtcttcacg cgctataaag aaaaagtaaa
      481 gtactggatg acgtttaacg aaatcaataa tcaggtgaat ttcagcgaaa gcctgtgtcc
      541 atttactaat tccggtatct tgtattcgcc agaggaagat atcaatgagc gcgaacaaat
      601 aatgtaccag gcggtacatt acgagttagt tgccagtgcc ctggcggtac agactggaaa
      661 atcgatcaat cctgaattta acattggctg tatgatcgcc atgtatccca tctatcctct
      721 gacgtgtgta cccaacgata tgatgatggc cacgaaagca atgcatcgtc gttactagtt
      781 tactgatgtt catgctcgtg gatattatcc gcaacatatg ctgaattact ttgccaggaa
      841 aggattcaac ctcgatatca caccagaaga taacgcgatt cttgccagtg gttgtgtcga
      901 ctttatcggc tttagctact acatgtcttt tacgacacaa ttttcgcccg ataacccgca
      961 actggattat gttgaaccac gagatttggt cagcaaccct tatatcgata catccgaatg
     1021 gggatggcaa attgatccgg cagggctacg ttattcactc aactggttct gggatcattt
     1081 ccagttgccg ctgtttattg tcgaaaatgg atttggtgcg gttgaccaga gacaagctga
     1141 cggcacggtg aacgatcact atcgcattga ttactttgct tcccatattc gggaaatgaa
     1201 aaaagccgta gttgaagatg gtgttgactt aattggctac accccgtggg gctgcattga
     1261 cctggtttct gccggaacag gggaaatgaa aaagcgctac ggaatgattt atgtcgataa
     1321 agacaacgaa gggaagggaa cgctggaacg gatacgtaaa gcgtcgtttt actggtatcg
     1381 ggatctcatc gccaacaatg gcgaaaatat ttgagacttg aggaagatat tacggagatg
     1441 tcaggctctg tgatcagagc ctgacaataa ctacatagcg tgttgattat aatagggcac
     1501 gccgagcgca tccgacttat cactgccagt acccatgtga tttaaatcaa acggtggttg
     1561 ttcctgcgat gggggaacaa ccacgacatt ggcacaagaa gtaacaactg gctgcgtggt
     1621 ttgttccgca acgccctgcg cggaaaagag aataagcgca gctgctattg cggatgaaag
     1681 tggtttcatg attgcctcag tgacgttgtc acgttatcaa ctagctattg taatgattta
     1741 atggatgtaa aaagcgcgat tcgccctgca tattggtcat ccggtattta tgtggcggaa
     1801 catcaaagta atttttgaag gttcgggtca gagtttgttg cgactcgaag ccatatcgtt
     1861 ctgccagata gagtatcggc tcgttacttt ccttcagctt ttgcgcgatt tccgtcatct
     1921 tacggctgcg gatgtattgg cctaatgaat gaccggtttc ttttttaaac atccgttgca
     1981 ggtgccattt agagtaacct gaacgctctg acactttctc cagtgacagt ggcgattcca
     2041 ggttgtcctc gatccagtcc aaaatgctat gaatggtaat agcgtcagta ttgcgtctgg
     2101 acatcgtcat acctcttttt tgtttacggc aggactttct taagcaaatg ctcaagtgtt
     2161 gccacttcgt ccgccgtcag gttttttgtt aattcttgat gcaggtcctg gccaactaat
     2221 tgatggcatt gttcgcatat tgccgcgccg ctggtggtaa gttttaccag tacgccgcgc
     2281 ttatcattcg ggttcggcaa cttttcaacc cagcctttac agaccaggcg atccagcata
     2341 cgggtcagtg ctccaaggtc gacagacaac acttttttca gttcaaccgg agtaatacac
     2401 gccgcgcagc ggatagagca gagcacctta aactgtgccg cggtaatatc cagcggagac
     2461 agatactcgt taagcagacg atctttcttc tgattaacca tatggattaa gcgacccaat
     2521 ggaataattt cattaaacag atcgctggta cttttcacat tagttgccct ggcaagtaat
     2581 tagttgcagg gggtaatatt gcccaggcaa gtataagtca aataaatgaa ttgaccgatg
     2641 ccacgttttg ctaaatcggt tcaagttttg gtcaatgcga tgcaaggtta gctaaagagt
     2701 gacactttat ccttgtttac cccttatact tttcgctgat aacccagata cactggataa
     2761 caaccaccaa tgttagattt gtttaaagca attggcctgg ggctggtggt gttactgccg
     2821 ttagctaacc cattaacaac tgtcgcgtta tttcttggcc tggcaggcaa catgaacagt
     2881 gccgaacgta atcgtcagtc gttgatggcc tcggtgtacg tatttgccat catgatggtg
     2941 gcgtattacg ccgggcaact ggtgatggat acatttggca tttcgattcc cggtctgcga
     3001 attgcaggcg gcttaatcgt cgcgtttatc ggttttcgga tgctctttcc gcaacagaaa
     3061 gcgattgatt caccggaggc gaaaagcaag tcggaagagc tggaagatga acccagtgcc
     3121 aatattgcct ttgtgccgct agcaatgcca agtaccgctg gtccgggaac catagcgatg
     3181 attattagct cggcgtccac agtgcgtcag agttcaacct tcgctgattg ggtactgatg
     3241 gttgcgccgc cgctgatctt tttcctggtg gcggttattt tgtggggaag cctacgcagc
     3301 tccggcgcaa ttatgcggct ggtgggcaag ggtggcattg aagcgatctc ccgtctgatg
     3361 ggattcctgc tggtatgtat gggtgtgcag tttattatta acggcatcct ggaaatcatt
     3421 aaaacgtacc attaaaaaag gcccgaatgg gcctttcaac tattgcgtct gttcttcgag
     3481 tgtcactggc cagcggcgaa atataatgat tgaccatatt aacgcggcaa aagcaggcac
     3541 cgcgcccaca taaccaatca tcgacattga ccagtgcaaa ctcacctgat tacctaccaa
     3601 cgcacccgcc ccgattccaa tattaaatat gccggagaat agcgccatcg cgacgtcggt
     3661 agcatctggt gccagcgcca gcactttaac ctgcatacca agcccgatga tcatcatcgc
     3721 gatcccccag aaaatactca gcaccccgag gtgtatttca ctgttcgccg caggtagcag
     3781 cagtgccaga cacaccagca acagcgcaat cgccgtactc accaacgcag acgcatactg
     3841 attacccagt ttaccgaaaa tcacgctgcc aataatgccc gcaccaccga gtaataacag
     3901 taatgccgtg gcaaagttgg cgctgaatcc cgcaatgttt tgcacaaaag gctcgatata
     3961 gctgtatgcc gtgtaatggg cggtgacaac caccacagtt aacaaataaa tgctcatcaa
     4021 tgccgggcgg cggaacaata gcgggaggct tttcaatgaa ccggaatgct cactgggcag
     4081 taagggaagt aacttaatca ggcacaaaag ggtgataagc gccccaatac caatcgcgaa
     4141 gaaggtcatt ctccaaccga aatactgccc cacaatgcgc ccgagaggta aacctaagac
     4201 catcgccagt gctgtaccgg tggcaattaa actcaaggcc tgtgcccgct tcccggccgg
     4261 agccatacgg atcgccagag acgccgtaat cgaccagaaa atcgcatgtg caaaagccac
     4321 accaatgcga ctgatcacca gaacggtaaa gctccacgat aaaaacgaca gtacgtggct
     4381 ggcaataaac accacaaaca ggcagatcag taatttgcgc cgttcaacct gactggtcat
     4441 taacataaaa ggcaatgaca ttagcgctac tacccatgcg taaatggtca acatgatgcc
     4501 gacctgagcg gtttgcatgt gaaaactttg cgcaatgtca gagagcaggc caacagggac
     4561 aaattcggtg gtgttgaaga tgaaggcggc gactgccagc gtaacgaccc gtagccacgc
     4621 cactttgcgg gaaacagtgt ttgttgtcat aacagattat cgggtgagtt gcgaaaaagt
     4681 gatgaaatga tcttaaaacg gttaatgatg tgaatacaat gcatttgtga tctggatcgc
     4741 gttttccgtt tctctgacgc tgattctgtt ccgcgtcaga gagtcggtaa catcacggtt
     4801 taatgacgtt cagggaggtc tggcgttttg gcggttgcgg gtataggtgg tctgacaggg
     4861 gagcgaggcc tgtttttcac caggtcttcg tgagtaactt ttggcggcgt ttcatttttt
     4921 acaaacagtt tgagggtcct gcgcgccata tgcttgataa ttttatcatc acttaatgat
     4981 gctggcatca acacgtcatt cagatcactt tcattgggat tcataaattg gtgatagtgc
     5041 aataacatat tggcgaaaaa taaaaaaaag tcctgaaaat gaggagattc ttgttctgct
     5101 aaaagggata aaaagcagat gcagacttcg ggtagtacat gtttgttaga acctaatgaa
     5161 tccatcaggc tttgcaataa atcctctttt aattcgccgt ctgttatgtc gagaatggcg
     5221 gtgatcaagt taggatcttt attgacgaac catctgtgaa cctcagagtt actccataat
     5281 cgggggagta tgacaaaatt accgcgagag ataaaattca ggacaagcaa atcaaactgg
     5341 cgtaagtaat ctgcagcggt atggtgatcc ggaggcaggg gttcaatatg tggtgtatgc
     5401 gcttttttga tggggacgcc aaggtggatc attggaaatt gttcaataaa atcattcaga
     5461 aacagaaaga gcacacgctc gacgccggtg tgaaatacgc gccccatagt gacaggcttt
     5521 gggttgggag cgcaaaaata ttaatttctc catcatggcg aaaattatct aaataaatag
     5581 ggtttaaatt tattactacc tgaaattcaa tattttcttt gtgtcttcca ttcttgacgg
     5641 gataagtgag tttaaactga accttttcat tgtatagtgt gtcgttaccc cgaaaagaag
     5701 tcctctgaat atatgataag ttatcaaggt tgttaaatat cttttttgtg gctgagttgg
     5761 tgtttaaaga cgtgcgccac tcttcatcac atattatggt actgagtgca tcaataaaat
     5821 tctttaaaac ttcttttacc tgaggtgcaa tattacgtac gggaaaatca tcaggaagaa
     5881 atatgtaatt actgatgctt actggcgtaa ccatatttgc cccatatagt cataagtcat
     5941 cggaacatca ctatataggg catttgagct gcaagaacta tcaaaaatag ctcattggta
     6001 accctgtttt gccgagtcag gcacatccag tagttgaata aatgcctcaa gcggacgcgt
     6061 tttcgcacca cgacgccaga ccagccaggt tgttaaccaa cgccattgct cagctaacgg
     6121 ccacgcttca acctggtgat gccccggcat actttccagc atagagcgtg gaataagcgc
     6181 aatgcctgct cctgcgacca cacaggccaa catcccgtga tacgactcca tctcgtgaat
     6241 agttcccgga gcggcaccgt cagcatgaaa ccagctctcg aagtggcgac gatacgaaca
     6301 attggcgcgg aaggcataaa tgttactgcc gttaacctga ctggcgcgga ttactggcgc
     6361 atgaccttgt ggcgtgacga tcatcagctc ctcgcggtat accggtatcc cgtcaatggc
     6421 agtatggtta atgggcccat caataaatgc cgcattcagt tttccctcca gcactccttc
     6481 cagcatagtg ccggaagggc cggtggaaag tgaaaactga attttgggat aacgatggtt
     6541 gaattccgcc agcgtggccg gaatgcgcac tgctgcggtg ctttccagcg aaccaagaga
     6601 aaataaacct tgcggctcat cgccagcgac aacgctccgc gcttcatcca ctaacgcgag
     6661 aatttgttgg ctatagcgta aaaagttatg cccggcagga gagagacgta aacgctgatt
     6721 ctcgcgaata aacagatcta cccccagttc tgtttccagc tggcgtagac ggatagtgag
     6781 attcgacggc acgcgaagca ctattgctgc tgcctgggtt atgctgccag cttcggcaac
     6841 cgcgttgaac atctccagtt gggtcagatc cataatattc tcgattcgtg aataagtggc
     6901 ttaatattat tcattttaaa gcaagagtaa atctgcgtat cttcatacca tgactcataa
     6961 aggagatacc ccgatgacca ttactccggc aactcatgca atttcgataa atcctgccac
     7021 gggtgaacaa ctttctgtgc tgccgtgggc tggcgctaac gatatcgaaa acgcacttca
     7081 gctggcggca gcaggctttc gcgactggcg cgagacaaat atagattatc gtgctgaaaa
     7141 actgcgtggt atcggtaagg ctctgcgcgc ccgtagcgaa gaaatggcgc aaatgatcac
     7201 ccgtgaaatg ggcaaaccaa tcaatcaggc gcgcgctgaa gtggcgaaat cggcgaattt
     7261 gtgtgactgg tatgcagaac atggtccggc aatgctgaag gcggaaccta cgctggtgga
     7321 aaatcagcag gcagttattg agtatcgacc gttggggacg attctggcga ttatgccgtg
     7381 gaactttccg ttatggcagg tgatgcgtgg cgcggttccc atcattcttg caggtaacgg
     7441 ctacttactt aaacatgcgc cgaatgtgat gggctgtgct cagctcattg cccaggtgtt
     7501 taaagatgcg ggaatcccgc aaggcgtata tggctggctg aatgccgaca acgacggtgt
     7561 cagtcaaatg attaaagatt cgcgcattgc tgctgtcacg gtgaccggaa gtgttcgtgc
     7621 gggagcggct attggcgcac aggctggagc ggcactgaaa aaatgcgtac tggaactggg
     7681 cggttcggat ccatttattg tgcttaacga tgccgatctg gaactggcgg ttaaagcggc
     7741 ggtagccgga cgttatcaga ataccggaca ggtttgtgca gcggcaaaac gctttattat
     7801 cgaagaggga attgcttctg catttaccga acgttttgtg gcagctgcgg cagccttgaa
     7861 aatgggcgat ccccgtgatg aagagaacgc tctcggacca atggctcgtt ttgatttacg
     7921 tgatgagctg catcatcagg tggagaaaac cctggcgcag ggtgcgcgtt tgttactggg
     7981 cggggaaaag atggctgggg caggtaacta ctatccgcca acggttctgg cgaatgttac
     8041 cccagaaatg accgcgtttc gggaagaaat gtttggccct gttgcggcaa tcaccgttgc
     8101 gaaagatgca gaacatgcgc tggaactggc taatgatagt gagttcggcc tttcagcgac
     8161 catttttacc accgacgaaa cacaggccag acagatggcg gcacgtctgg aatgcggtgg
     8221 ggtgtttatc aatggttatt gtgccagcga cgcgcgagtg gcctttggtg gcgtgaaaaa
     8281 gagtggcttt ggtcgtgagc tttcccattt cggcttacac gaattctgta atatccagac
     8341 ggtgtggaaa gaccggatct gacgcgacat attcagctct gatatactcg caggtctttt
     8401 cagacctgcg gtccaggagt agaaagtggc agtcgccatg gataatgcaa ttttagaaaa
     8461 catcttgcgg caagtgcggc cgctcattgg tcagggtaaa gtcgcggatt atattccggc
     8521 gctggctaca gtagacggtt cccgattggg cattgctatc tgtaccgttg acggacagct
     8581 ttttcaggcc ggagacgcgc aagaacgttt ttccattcag tctatttcca aagtgctgag
     8641 tctcgttgtc gccatgcgtc attactccga agaggaaatc tggcaacgcg tcggcaaaga
     8701 tccgtctgga tcaccgttca attctttagt gcaactggaa atggagcagg gtattccgcg
     8761 taatccgttt attaatgctg gtgcgctggt ggtctgcgat atgttgcaag ggcgattaag
     8821 cgcaccacgg caacgtatgc tggaagtcgt gcgcggctta agcggtgtgt ctgatatttc
     8881 atacgatacg gtggtagcgc gttccgaatt tgaacattcc gcgcgaaatg cggctatcgc
     8941 ctggctgatg aagtcgtttg gcaatttcca tcatgacgtg acaaccgttc tgcaaaacta
     9001 ctttcattac tgcgctctga aaatgagctg tgtagagctg gcccggacgt ttgtctttct
     9061 ggctaatcag gggaaagcta ttcatattga tgaacctgtg gtgacgccaa tgcaggcgcg
     9121 gcaaattaac gcgctgatgg cgaccagtgg tatgtaccag aacgcggggg agtttgcctg
     9181 gcgggtgggg ctaccggcga aatctggcgt tggtggcggt attgtggcga ttgttccgca
     9241 tgaaatggcc atcgctgtct ggagtccgga actggatgat gcaggtaact cgcttgcggg
     9301 tatcgccgtc cttgaacaat tgacgaaaca gttagggcgt tcggtttatt aatgcagtct
     9361 ctcgatccac tcttcgcgcg tttatcccgt tcaaaatttc gctctcgctt tcgtctgggc
     9421 atgaaagagc gtcagtattg cctggagaaa ggcgcgccag tcatcgaaca acatgcggcg
     9481 gattttgtcg ctaaacgtct tgctccggca ttaccggcta atgacggtaa gcaaacaccc
     9541 atgcgcgggc atccggtgtt tatcgctcag cacgccaccg cgacctgctg tcgcggttgt
     9601 ctggctaagt ggcataacat tccgcagggt gtttcgttaa gcgaagaaca acaacgttat
     9661 atcgtggcgg taatctatca ctggctggtt gtacagatga atcagccatg agaattgcgg
     9721 aaaacgataa aatagcattc ccagggtacc tttatttgcc gccgctgtta gcgaacatgc
     9781 tatcctttat gagaatttac ttattacgtt gagataattg ccacccgcaa ttgtatggac
     9841 agataattaa tgcatgtaca ccccatttca actttccgtt tgttccagga aggtcatctg
     9901 ctacgtaata gcatcgctat ttttgcgcta accacgctgt tctattttat tgttgcagag
     9961 ttacggctgg ttcacgaact ttctcttttc tggccgctga atggcgtaat ggcgggggtg
    10021 tttgcccgct atgtctggct taatcgactg cattactatg cgatcagtta tgtggcgatg
    10081 ctggtttatg atgccataac caccgaatgg ggactggttt cactggttat caatttctcc
    10141 aatatgatgt ttattgttac cgtcgcctta ctggtcgtgc gggataagcg tctggggaaa
    10201 aataagtatg agccagtaag tgccttacgg ctatttaatt actgtctgat tgccgcatta
    10261 ttatgcgcta ttgtcggggc gattggttcg gtcagtattg atagtctgga tttctggcct
    10321 ttgcttgcgg actggttcag tgagcaattc tcaacgggcg tgttgatcgt gccttgtatg
    10381 ctgacgttgg ctattcctgg agtgctgccg cgctttaaag cagaacagat gatgcccgcc
    10441 atcgcgctta ttgtgtcggt tattgcctcg gtagtcattg gcggagcggg gagtctggcg
    10501 ttcccgctgc ctgcattaat ctggtgtgca gtgcgctata cgccgcaggt aacatgtctg
    10561 ttgacctttg tcaccggtgc ggtggaaatc gtactggtgg caaattcggt gattgatatc
    10621 tcggtcggtt cgccgttctc cattccggaa atgttctccg cacgtctcgg tattgccacg
    10681 atggcgatat gcccaattat ggtttctttt agcgtggcag cgatcaattc gctaatgaag
    10741 caagttgcgc tgcgagccga ctttgatttt ctgactcagg tttactcacg gtccggtctt
    10801 tatgaggcgc tgaaaagtcc atcgctgaaa cagacacaac atctgacggt catgctgctt
    10861 gatatcgact atttcaaaag cattaacgat aactatggac atgaatgtgg cgataaagtg
    10921 ttaagcgtgt ttgcccggca tattcagaag attgtcggtg ataaggggct ggtggcgcgc
    10981 atgggcggcg aagaatttgc tgttgcagtg ccgtcggtga atcctgtcga tggtctgcta
    11041 atggcggaaa aaatccgtaa aggcgttgaa ctgcaaccat tcacctggca acaaaaaacg
    11101 ctttatctca cggtaagtat tggcgtcggt agtggtcgcg catcgtaccg aacgctgacc
    11161 gatgatttta ataaattgat ggtcgaagcc gatacgtgtc tgtatcgctc gaagaaagat
    11221 gggcgcaacc gtaccagcac tatgcgttac ggtgaggaag ttgtctgaca tctttattca
    11281 ttccgcgcga tttgaatgca atgtgttcta ccactttatg atccaggaaa aggcgtattt
    11341 cctcgattaa accatgatcc gcgccacact tttctcataa aactgctcat taatcatcag
    11401 gagttgatca aaacaggggt tcggcgtatt aaattttgct aacctgtcgt accaattcaa
    11461 acgggttgta tgactaatca gaagggaacc cattgtgaaa acactaaatc gtcgcgatat
    11521 tcccggtgca cagtatccag aacgtatcat tcagtttggt gaaggtaact tcctgcgcgc
    11581 ctttgttgac tggcaaatcg atctcctgaa tgagcacacc gatctgaatt ctggcgtggt
    11641 cgttgttcgt ccgattgaaa cttcattccc gccgtcactg agcacgcagg atggtctgta
    11701 caccaccatt atccgtggcc tgaatgagaa ggggtgaagt ggtcaacaaa aactggccac
    11761 cgagttagag tttttccagt atcg
//
LOCUS       DADLTD010000137        11576 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLTD010000137 DADLTD010000000
VERSION     DADLTD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.137"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..985)
                     /locus_tag="JN701_002883"
     CDS             complement(<1..985)
                     /locus_tag="JN701_002883"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY7365270.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRENIMLEGFTLFTDWL"
     gene            complement(1194..1853)
                     /locus_tag="JN701_002884"
     CDS             complement(1194..1853)
                     /locus_tag="JN701_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY7365271.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2180..2536)
                     /gene="yebF"
                     /locus_tag="JN701_002885"
     CDS             complement(2180..2536)
                     /gene="yebF"
                     /locus_tag="JN701_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY7365272.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2603..2897)
                     /gene="yebG"
                     /locus_tag="JN701_002886"
                     /pseudo
     CDS             complement(2603..2897)
                     /gene="yebG"
                     /locus_tag="JN701_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3031..4209
                     /gene="purT"
                     /locus_tag="JN701_002887"
     CDS             3031..4209
                     /gene="purT"
                     /locus_tag="JN701_002887"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY7365273.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4265..4906)
                     /locus_tag="JN701_002888"
     CDS             complement(4265..4906)
                     /locus_tag="JN701_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY7365274.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4943..6754)
                     /gene="edd"
                     /locus_tag="JN701_002889"
     CDS             complement(4943..6754)
                     /gene="edd"
                     /locus_tag="JN701_002889"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY7365275.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(6989..8464)
                     /gene="zwf"
                     /locus_tag="JN701_002890"
     CDS             complement(6989..8464)
                     /gene="zwf"
                     /locus_tag="JN701_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY7365276.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8802..9671
                     /locus_tag="JN701_002891"
     CDS             8802..9671
                     /locus_tag="JN701_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7365277.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9799..11241
                     /locus_tag="JN701_002892"
     CDS             9799..11241
                     /locus_tag="JN701_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase II"
                     /protein_id="HAY7365278.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11301..11519)
                     /locus_tag="JN701_002893"
     CDS             complement(11301..11519)
                     /locus_tag="JN701_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365279.1"
                     /translation="MEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGM
                     RRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         2804 a         2982 c         2892 g         2898 t
ORIGIN      
        1 ccagccagtc ggtaaacagc gtaaaccctt ccagcatgat gttttcgcgt ggcggaatta
       61 actcttccca ctgttgctca tcacgcaggc gggtacggta taagccaaag tttttgccgt
      121 ggcggttgga acgcagataa aaccgatgct ggtagtgatc aaggctgtat tcgtgatctt
      181 tgcggcgcgg cagaaaaaca aacggctcgg catcggccat ttccgcgtcc agcaggcgaa
      241 cttcactggt ggtggcgctg gccaaatgaa tgactacata gtgcttcgag gtcgttttat
      301 gcaggctgac gtaataggta tcgtcttttt cttcgtagat cagtttatct tgcgatgctg
      361 gcgtaccgat ggcgtgacgc cagacctgat aaggcagcag cgtcaccgga tgcttgcgaa
      421 catagtagaa agtccaggag tcatttgccc agacaaagct gggttcaacg ttatccagca
      481 gttccgggta ccagttacca gtttccagat tacgaaaacg aatgccgtac tggcgtcggg
      541 aaagaaaatc ttctgccagc gccataatgg tgttatcggg cgtaatcgcc attccgccca
      601 tcgaataaaa ctcactatga gctgcgcgct tattggcatc gagcaatgtt tcccactcat
      661 cccactcttc actgaatgcc gattgacgct ggtagatagc atattcacag cctggttcat
      721 aaatatgccg atagcggtag ccatttttga tgtagggcgc agaaacttct cgttgcggaa
      781 tgcggtcgat gatttccttt aagatgcgat cctgcaaggc ttgttgtgag gccatcaccc
      841 gatgaccgta actattttct tgttgcaggt agtccaggac ttctggctga gaacgcgtat
      901 cgtcccgcag ccagtagtaa ttatcgatgc gcgtatcgcc atgaagcgtc atggcgtggg
      961 gaatgcgggc ggcttttggt agcatgttat tgttctttct ggttgaaaca tcttataagg
     1021 gtggcaaaac tcaccgggga tgcaagcgaa acaggggagt cattgcttag atgatgacag
     1081 gtaatggcgc ggatatcgaa tgttatgcag acagagaaaa tcagcctgtt caaatggctg
     1141 tgcgattctg gatagcccga aatagtcaac ttcaggctat ccagagagcg gaattattcc
     1201 gccaaagtgc gtttttgctg ttcgagatcg cgttcaatgc cgtcacgaac atcctggggg
     1261 attttcagcg cgtcacccag tgcattcagg taactgcgtt ccataaaatg gtcaatatca
     1321 atagccgcgc aactcaggaa atagatttcc agcgcctctt cttcattgcg gaccccggta
     1381 gccaagcgtt gtggatccag cggttgttcg attgcctgct caatgagtac acgcccctgc
     1441 tcttccacgc cggcttcacg caattgctgg tcgattgccg cacgttcttt ggcatcaata
     1501 tgaccatcac ttttagcggc aaagaccagc gcaaggatca aacgttctgt acgctcatcc
     1561 agcggcgtac tttgcgcgcc aaactgcggt tcgtcctgat gcgccgcgcg aattttatct
     1621 ttgtatttat tccacagcac cgtacccgct accgctccgc cgccaaccag taacgcgttg
     1681 gtgccatatt tcgtaagaag tttacgtgct gatttatttg cgaccagcag cccagccagt
     1741 ccgcctaatg cgcctggcac taacagtttg cccaatccct gatccgcaga cgaggagata
     1801 gaagaactgc tttgcccaag cagggattgc agttgattta accagttagc catatttgct
     1861 cctcaataac catttttagt gcctgtagca tagcggatga ggatgtcaga aaatgtatat
     1921 cgagacaaaa gatgcgcaaa taagcggggc ggtaggccgt tattcaaaga aattcgcgcc
     1981 aggtaaggta tttactgtat atcaggtgat aagggtattt taggtgaaag tagtttgtcg
     2041 ttttgagtgg cattgtttga tgctgattgc ccgatgcgac gctgttgcgt cttctcaggc
     2101 ctacgattgt ggtgcgttct ggggcggaca aggcactcgc gccgcatccg ccaacggtca
     2161 atcgaattat cagaagccat taacgccgct gatattccgc cattcccgct ttgcagtcca
     2221 cgctgacctg gtaatgaata tcggcacttt taccacgcac ggttagcggt actgaccatt
     2281 tatcatcttt accctgaatg tcctgcaaac tgacccaagc cacgggatcg gcctgaccga
     2341 caattttttg atcatctgcc caacgcgcca cgcgattttg ttgataatca cgttttacgc
     2401 tcgcggcaat tccggcagca tccagacctt cacactttgg gaaagtgacc gacttgctgg
     2461 tttcattatt ggcagcgaaa actgatgcgc aggcagaaac caacaacagc cctaaaaacg
     2521 cccctctttt tttcatgttt ttctccatag cacaatgatt caggagaaag catggtacaa
     2581 attgtcagga gcgcaagttg cttcaggccg cgtgtgaggc atcttcctct tcggattcag
     2641 caccgacgac ctgtgggtgg ggacggtaat ttgccggttt tcagaatggt gctcagcaca
     2701 tctttttgtt ctgccagcca tagcgaaagg gcttcacgtt gctcgtcttc catttgcact
     2761 ggagaattag tcagccaggt gtcaagcaga tccgccgtat caagcatttt gtcataagca
     2821 tcggcttcct ttttgctggt aaacgacatt ttctcttcgc cctcacgaat gactacgtat
     2881 ttaacttcaa ccgccatttg cagcctctca taataactgt gattttatac agtatatttc
     2941 ttttcggttg agaaatcaaa atcaacaata aagacacacg caaacgtttt cgtttatact
     3001 gcgcgcggaa ttaatcaggg gatattcgtt atgacgttat taggcactgc gctgcgtccg
     3061 gcagcaactc gcgtgatgtt attaggctcc ggtgaactgg gtaaagaagt ggcaatcgag
     3121 tgtcagcgtc tcggcgtaga ggtgattgcc gtcgatcgct atgccgacgc accagccatg
     3181 catgtcgcgc atcgctccca tgtcattaat atgcttgatg gtgatgcatt acgccgtgtg
     3241 gttgaactgg aaaaaccaca ttatatcgtg ccggagatcg aagctattgc caccgatatg
     3301 ctgatccaac ttgaagagga aggactgaat gttgtcccct gcgctcgcgc aacgaaatta
     3361 acgatgaatc gcgagggtat ccgtcgcctg gcggcagaag agctgcagct gcccacttcc
     3421 acttatcgtt ttgccgatag cgaaagcctt ttccgcgagg cggttgctga cattggctat
     3481 ccctgcattg taaaaccggt gatgagctct tccggcaagg ggcagacgtt tattcgttct
     3541 gcagagcaac ttgctcaggc atggaagtac gctcagcaag gcggtcgcgc cggagcgggc
     3601 cgcgtaattg ttgaaggcgt cgttaagttt gacttcgaaa ttaccttgct aaccgtcagc
     3661 gcggtggatg gcgtccattt ctgtgcaccg gtaggtcatc gccaggaaga tggcgactac
     3721 cgtgaatcct ggcaaccaca gcaaatgagc ccgcttgccc ttgaacgtgc gcaggagatt
     3781 gcccgtaaag tggtgctggc actgggcggt tatggtctgt ttggtgtcga gctgtttgtc
     3841 tgtagtgatg aggtgatttt cagtgaggtc tcccctcgtc cacatgatac cgggatggtg
     3901 acgttaattt ctcaagatct ttcagagttt gccctgcatg tacgtgcctt cctcggactt
     3961 ccggttggcg ggatccgtca gtatggtcct gcagcttctg ccgttattct gccacaactg
     4021 accagtcaga atgtcacgtt tgataatgtg cagaatgccg taggcgcaga tttgcagatt
     4081 cgtttatttg gtaagccgga aattgatggc agccgtcgtc tgggtgttgc gctggcgacc
     4141 gctgagagtg ttgttgacgc cattgaacgc gcgaagcacg ccgccggaca ggtaaaagta
     4201 cagggttaaa cccggcaaaa aaacgctaca aaaatgcccg atcgtagatc gggcgttttg
     4261 acttttacag cttagcgcct tctacagctt cacgcgccag cttagtaatg cggtcgtaat
     4321 cgcccgcttc cagcgcatct gccggaacca gccaggaacc accgatgcac agcacgcttt
     4381 tcagcgccag gtagtcacgg tagttagccg gagaaatacc acccgtcggg cagaaacgga
     4441 cctgggagaa cggacccgcg atcgcctgca gggctttcac gccgccgtta gcttcagccg
     4501 ggaagaattt gaactctttc aaaccgtagt ccatacccag catcagttcg gaaacagtgc
     4561 tgatccccgg aattagagga atagtccctt cggtagcagc tttcagcagc ggctcggtca
     4621 gacccgggct aattgcgaac tgtgcacccg cttcagtgac ttctgccagc tgctgtggat
     4681 tcagcaccgt accggcaccc acaatcgctt caggcacttc tttggcgata gcacggatag
     4741 cgtcaactgc acactcggta cgcagagtca cttccagaac gcgcacccca ccagcaacca
     4801 acgcttttgc catcggcact gcgtgttcca gtttttttac cacgataacc ggtacaaccg
     4861 ggccggtggt caggattgat tctgcacttg ttttccagtt tttcatcaga gttttctctc
     4921 gcctgattac aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg
     4981 acagtttttc acgcaaggcg ctgaataatt cacgtcctgt ccccacgcgt gacgcgctca
     5041 ggtcaggaat gtgcggttcg cgagcagcca gttccgcttc gtctaccagc agcgtcagtt
     5101 cgcctgtctg tccattcaca cgaatgatgt ccccgtcgcg cacttttgcc agcagcccgc
     5161 catcgtaggc ttctggtgtt acgtggatag ctgacggcac tttacctgaa gcgccggaga
     5221 gtcgtccatc ggtaactaac gcaattttga aacaccggtc caataataca ccaagtggcg
     5281 gcatgagttt atgtaattct ggcattccgt tcgcttttgg cccctgatga cggacaacaa
     5341 cgacacaatc gcggtccagc aaacccgctt caaaggccgg cataacgtca tgctggcttt
     5401 caaaaacaac cgctggcgct tcaatcacct ggttctcaac cggcacggca gaggttttca
     5461 taaccgcacg gcccaggtta ccgcttaaca cttttgtccc accatgatga gagaaaggtt
     5521 gttcgaagga agcgatcaca ttgctgtcga gtgatttttc cgccccttcc cgccagtcca
     5581 gttcaccatt attcagccat ggttcaaggg tataacgaga cagaccaaag cctgccaccg
     5641 tattgacatc ttcatgcagc aggcctgctt tgagcagttc acgcaccaga accggtacgc
     5701 cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca
     5761 gcggtacaac atcagaaagg tcagagaagt catcccagtt aatctgaata ccggccgcgc
     5821 gcgccatcgc caccaggtgc atggtgtggt tagtggaacc accggtcgcc agcagtgcaa
     5881 cgataccgtt caccaccact ttctcatcga tcatcttacc gatcggcatc cattcattac
     5941 cattaccggt catgcgtgta acctgacgcg cagcggcagc ggtcaaagca tcgcgcagcg
     6001 gagaatccgg atgaacaaaa gaagagcctg gcgactgcat ccccataaat tccaccacca
     6061 tctggttggt gttggcagta ccgtagaaag tacatgttcc cggcgcatgg taagacgcgg
     6121 cttctgactc cagtaaggcc atgcggtcca ctttaccttc ggcataaagc tggcgaatac
     6181 gcactttttc tttatttggc aaaccgcttg ccatcggtcc agacggcaca aacaccgcag
     6241 gcaaatgacc aaacgacagg gctgccatcg tcagacccgg gacaatcttg tcgcacacac
     6301 cgaggaacag agcaccatca aacatgttat gggacagccc caccgccgca gacatcgcta
     6361 tcacttcgcg gcttagcagc gacaattcca ttccatcctg cccctgggtg acaccatcac
     6421 acatcgccgg aacaccgccc gcaacctgac caaccgcatt cgcttcatgc agggctttac
     6481 gaatgatttc tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg ttataggagg
     6541 tgatgatggc gatattgtta cgcaacatgc ttttcaaaga ggctttgtct tctggctggc
     6601 aggcagcgaa accgtgtgcc aggttaccgc atgccaactg cgaacgatga acggtcgacg
     6661 ttttcgcttg ttctatccgg gcgagataag cagagcgagt ctcgcgcgaa cgttcaatga
     6721 ttcgatttgt tacgcgtaac aattgtggat tcataaaggc tcctgaaatt gagttgtcag
     6781 agcaggatga ttcacaacgc gtttcattca gaggatttat gagtgaaacg cctgtaaccg
     6841 gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc
     6901 gcacgaggcc tgaaagtgta aaaattgttc tacaatctgt gcaagatcat gttaccggta
     6961 aaataaccat aaaggataag cgcagatatt actcaaactc attccaggaa cgaccatcac
     7021 gggtaatcat cgccaccgag gcaacgggtc cccaggttcc ggcctgatac ggtttcggcg
     7081 catcattgtc catcgcccac gcctcagtaa tggagtctac ccatttccag gcttcttcca
     7141 cttcatcacg acgtacaaac agtgcctgaa taccacgcat ggtttccagc agcagacgtt
     7201 cataggcatc cgccagatgc gtctgattaa aggtttctga atagctcaga tccagcttgg
     7261 tgatttgcag gttatgtttg tggtcaaggc caggaacttt attcagtacc tggatatcca
     7321 cgccttcatc aggttgcaga cggatagtca gtttattctg cggcagatcc tgccacgact
     7381 ctttaaacag attcagttca ggtgttttga aatagaccac gacttcagaa catttggtcg
     7441 gcagacgttt accagtacgc aggtagaatg gcacaccggc ccagcgccag ttatcaatgt
     7501 cgacgcggat cgccacgaag gtttctgtat tgctgctctt gttcgcaccc tcttcttcca
     7561 gatatcccgg cacttttttg ccctgggcga agcccgcagt atattgccca cgtacggttt
     7621 tttcgcgtac gttggagcgg tcgatgcggc gcagggactt cagtactttc actttttcat
     7681 cgcggatgct gtctgcgctc aggtcagatg gcggagacat cgcaatcatg cagagaattt
     7741 gtagcaggtg gttctgaatc atgtcacgca tctgaccggc tttatcaaaa tagccccagc
     7801 gcccttcgat ccccacttct tctgccacgg taatctcaac atgatcaatg gtgcgattgt
     7861 cccagttatt cacaaacagg gagttagcaa aacgcagcgc caacaggttc agcaccgttt
     7921 ctttaccaag atagtggtcg atacggtaaa cctggcactc ctcgaagtat tcgccaacct
     7981 gatcattgat ttcctgcgag gtcgccagcg acgtccccag cggtttctcc atgactacgc
     8041 gtgccggttt agcattcagt tttgcctcgc caagcccttt gcaaattgcg ccaaaagtgc
     8101 tgggcggcat ggcaaagtag ttaatggtga tacgattttt ttgatccagc atcgcgccga
     8161 gacggttgaa tgcagcagtg tcattgacat cgagattaca aaaatccaga cgtgcactca
     8221 gggtgtccca taaaccttca tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga
     8281 caactttggt atatgccgct ttatcccagt cagcacgccc tacgccgata atccgggtgt
     8341 ccgggttgag ctgaccggct ttttccagtt gatacaggga aggcagcaat ttacgacgcg
     8401 caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg
     8461 ccatgtcatt ctccttaagt taactaaccc ggtacttaag ccagggtata cttgtaattt
     8521 tcttacggtg cactgtactg cttttacgag cttgcgaaaa ctgtaaacgc ttatccaccc
     8581 gtgcgattac gggaaaagcg cgcaaagcgc ggcaaaactg ataaaaaaat catcgttttc
     8641 cattcggtaa aaatctgaca ctaatcatgt tatgaaaaaa aataacaact tttttatctg
     8701 cttttgtcat taacacggca cacaggcgta atatctgaca aaactgcatt tcgatttctt
     8761 tcagtgcgga aatcgtcatt acccgtgagt ctctttacat catgaatatg ctggaaaaaa
     8821 tccagtctca gctggaacat ttgagcaaat cagagcgcaa agttgccgag gtcattctgg
     8881 cttcgcccga taacgcgatc cattcgagta ttgctgctat ggcactggaa gccaatgtta
     8941 gcgaaccgac ggtgaatcgt ttctgtcgca gcatggacac gcgcggtttt cctgatttta
     9001 aacttcatct ggcacagagt ctggcgaatg gcactcccta tgttaatcgc aatgtcaatg
     9061 aagatgacag cgttgaatca tacacaggga aaatatttga gtccgcaatg gcaacgcttg
     9121 atcatgtccg tcattcactg gataaatctg ccatcaaccg cgccgtcgac ttgctcactc
     9181 aggcaaaaaa aatcgccttt ttcggattag gctcttcagc cgccgttgcc cacgatgcga
     9241 tgaataagtt ctttcgtttt aatgttccgg tggtgtactc cgatgatatc gtgctgcaac
     9301 gcatgagttg tatgaattgt agcgacggag acgtggtggt gctgatttct cacactggaa
     9361 gaacaaaaaa tctggtcgag ctggcgcagc tggctcgcga aaacgacgca atggtgattg
     9421 ccctcacctc tgcgggtacc ccgctcgccc gggaagcaac gctggcaatt accctcgacg
     9481 taccggaaga tactgacatt tatatgccca tggtttctcg acttgcacag ctgaccgtga
     9541 tagatgtgct ggcgacagga tttactttgc gacgcggtgc aaaattcaga gataacttga
     9601 agcgggtcaa ggaagcgctg aaggaatcgc gttttgataa gcagttactt aatttaagtg
     9661 acgatcgcta aaaacgactg tcactgtcct aatcttatac gacatccgaa tgagattaat
     9721 ttatcgccat cgcggcgtta tttcattcgg atttcatgtt caagcaacac ctggttgttt
     9781 cagtcaacgg agtattacat gtccagaagg cttcgcagaa caaaaatcgt taccacgtta
     9841 ggcccagcaa cagatcgcga taataatctt gaaaaagtta tcgcggcggg tgccaacgtt
     9901 gtacgtatga acttttctca cggctcgcct gaagatcaca aaatgcgcgc ggataaagtt
     9961 cgtgagattg ccgcaaaact ggggcgtcat gtggctattc tgggtgacct ccaggggccc
    10021 aaaatccgtg tatccacctt taaagaaggc aaagttttcc tcaatattgg ggataaattc
    10081 ctgctcgacg ccaacctggg taaaggtgaa ggcgacaaag aaaaagtcgg tatcgactac
    10141 aaaggcctgc ctgctgacgt cgtgcctggt gacatcctgc tgctggacga tggtcgcgtc
    10201 cagttaaaag tactggaagt tcagggcatg aaagtgttca ccgaagtcac cgtcggtggt
    10261 cccctctcca acaataaagg tatcaacaaa cttggcggcg gtttgtcggc tgaagcgctg
    10321 accgaaaaag acaaagcaga cattaagact gcggcgttga ttggcgtaga ttacctggct
    10381 gtctccttcc cacgctgtgg cgaagatctg aactatgccc gtcgcctggc acgcgatgca
    10441 ggatgtgatg cgaaaattgt tgccaaggtt gaacgtgcgg aagccgtttg cagccaggat
    10501 gcaatggatg acatcatcct cgcctctgac gtggtaatgg ttgcacgtgg cgacctcggt
    10561 gtggaaattg gcgacccgga actggtcggc attcagaaag cgttgatccg tcgtgcgcgt
    10621 cagctaaacc gagcggtaat cacggcgacc cagatgatgg agtcaatgat tactaacccg
    10681 atgccgacgc gtgcagaagt catggacgta gcaaacgccg ttctggatgg tactgacgct
    10741 gtgatgctgt ctgcagaaac tgccgcaggg cagtatccgt cagaaactgt tgcagccatg
    10801 gcgcgcgttt gcctgggtgc ggaaaaaatc ccgagcatca acgtttctaa acaccgtctg
    10861 gacgttcagt tcgacaatgt ggaagaagct attgccatgt cagcaatgta cgcagctaac
    10921 cacctgaaag gcgttacggc gattattacc atgaccgaat cgggtcgtac cgcgctgatg
    10981 acctcccgta tcagctctgg tctaccaatt ttcgccatgt cgcgccatga acgtacgctg
    11041 aacctgactg ctctctatcg tggcgttacg ccggtgcact ttgatagcgc taatgacggc
    11101 gtagcagctg ccagcgaagc ggttaatctg ctgcgcgata aaggttactt gatgtctggt
    11161 gacctggtga ttgtcaccca gggcgacgtg atgagtaccg tgggttctac taataccacg
    11221 cgtattttaa cggtagagta atctcacctg ccccggccgc tgatggccgg ggtatttagc
    11281 aacacataaa ttaggactac ttattcaatc caatcataat cacagccccc tgtaaaatga
    11341 atacagggat agcctgaatg gagatctaaa tagcttgcaa gacgtcgcat acccgcttcc
    11401 tctgcaacac tatgtccaat aactgccatc ggtaagccac tatctttgga gtattgtgtc
    11461 tgcacccatg ttttaattga atcgttaatc gtaatataat aatcagcctg atattccata
    11521 tattgtattg gatcacaaaa acagcctgcc ccaatgccaa cagttctgac tactct
//
LOCUS       DADLTD010000138        11405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.138, whole genome shotgun sequence.
ACCESSION   DADLTD010000138 DADLTD010000000
VERSION     DADLTD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.138"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..921
                     /gene="gap"
                     /locus_tag="JN701_002894"
     CDS             <1..921
                     /gene="gap"
                     /locus_tag="JN701_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7365280.1"
                     /translation="DVVAINDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKS
                     IAVYAEKEAKNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTI
                     VYNVNDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVD
                     GPRGKDLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVS
                     ILGKKATAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGD
                     LQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(963..2403)
                     /gene="aldA"
                     /locus_tag="JN701_002895"
                     /pseudo
     CDS             complement(963..2403)
                     /gene="aldA"
                     /locus_tag="JN701_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2558..3400)
                     /locus_tag="JN701_002896"
     CDS             complement(2558..3400)
                     /locus_tag="JN701_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY7365281.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3672..7574)
                     /gene="hrpA"
                     /locus_tag="JN701_002897"
     CDS             complement(3672..7574)
                     /gene="hrpA"
                     /locus_tag="JN701_002897"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY7365282.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLCFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLTEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISYDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7775..8380
                     /gene="azoR"
                     /locus_tag="JN701_002898"
     CDS             7775..8380
                     /gene="azoR"
                     /locus_tag="JN701_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY7365283.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8431..9747)
                     /locus_tag="JN701_002899"
     CDS             complement(8431..9747)
                     /locus_tag="JN701_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HAY7365284.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9737..11405)
                     /locus_tag="JN701_002900"
                     /pseudo
     CDS             complement(9737..11405)
                     /locus_tag="JN701_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2847 a         3094 c         2711 g         2753 t
ORIGIN      
        1 gacgttgtcg ctattaatga tctcacttcc ccaaaaattc tcgcctacct gctgaaacat
       61 gattcaaact acggaccatt cccctggagc gttgatttta cggaagattc acttatcgtt
      121 gatgggaaaa gtatcgcggt ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg
      181 aaaggtgcag aaatcattgt cgaatgtact ggcttttata cctccgccga gaaatcgcag
      241 gcgcatcttg atgctggtgc gaagaaggtg ttgatttccg cccctgccgg tgaaatgaaa
      301 actatcgttt ataacgtcaa tgacgacact ctggatggca acgacaccat tgtttccgtg
      361 gcgtcatgca ccactaactg tcttgcgccg atggccaaag ccttgcatga cagtttcggg
      421 atagaagtcg gcacgatgac gaccattcat gcctatactg gcacccagtc actggtggat
      481 ggcccacgtg gtaaagattt acgtgcttca cgcgcagcgg cagaaaatat cattccccac
      541 actacggggg cggcaaaagc cattggtctg gagatcccgg aactgagcgg caaactgaaa
      601 ggtcatgcgc aacgcgtgcc ggtgaaaaca ggttcggtca ctgaactggt atcgattctc
      661 ggaaaaaaag cgactgccga agaggtgaat aacgcactta aacaagcaac caccaataac
      721 gagtcatttg gttataccga tgaagaaata gtctcttccg atatcattgg cagccatttc
      781 ggttcggtgt ttgatgccac gcaaacggaa attaccgccg tgggcgattt acaactggtg
      841 aaaacggtcg cctggtacga taacgaatat ggcttcgtca cgcagcttat tcgcaccctc
      901 gaaaaattcg ctaaactctg acgcgcacag gcggaggaaa aaacctccgc ctcttttact
      961 cattacgact gtaaataaac cacctgggtc tgcagatatt catgcaggcc atgtttacca
     1021 tctgcgccgc caataccgga tttacgccat ccggcgtgga agccttgcat agcttcgaag
     1081 ttttcacggt tgatgtaagt ttcaccaaac ttcagccctt taatggcttt catcgcgacg
     1141 ttcagatttt gggtatagat ttgatgaggt caggccgtaa tcactgtcat tagccattga
     1201 gatagcctct tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc
     1261 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     1321 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     1381 cactttttgc tcaacccttt ccaacgccgc ggcgttaatc aacggcccca tcgcaatgtc
     1441 gttgcgttca gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac
     1501 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     1561 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     1621 cattacgata gctggtgctt taccccccaa ttccagacac actttggtga tgtttttcgc
     1681 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     1741 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     1801 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     1861 cgtaaattca ctaggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     1921 gcgggcaatg aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc
     1981 acgtttaaac aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     2041 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     2101 agccagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     2161 ttcgcggatc ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc
     2221 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     2281 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     2341 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     2401 catgggcgac tcctgtgatt tatatgtttg ttcttggtaa atacattgtt aattaacatc
     2461 aatcatcata gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag
     2521 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     2581 gttcacaaac gggcaatttt tgacgtaacg agcgactttc catcgcctcg atgagcaccg
     2641 catcgtgttt aagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     2701 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc
     2761 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     2821 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     2881 gtgcatcggg attgtcccca gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg
     2941 tggggtcctg aacaacgata gccgtatgaa ctcgttctac ggcctgatta aatagcgcga
     3001 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     3061 gcggaatgtg ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag
     3121 tggtgcggat agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg
     3181 agtgaccgat accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg
     3241 ccgcgtcgat agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg
     3301 gcacctcacc ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta
     3361 tagttgccgg cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc
     3421 ttaagaatac tgcgtgagat aatagtgaga agctgagcac tctgaaagga ggaagtcgta
     3481 tgtccttaac cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac
     3541 attattcatc ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc
     3601 gttttagaat taataaaaaa cccggcgcta aatgcttacc gggttttaga tttattaggc
     3661 aaatagcagg gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc
     3721 ggataaggcg taccaagttg ttgagcgaag taactaacgc gcagctcttc tatcatccaa
     3781 cggatctctt tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt gatccattgc
     3841 tgccacgcct gctggacgtt ttcgactttc agcatctgtg cacgatcgcg atgtggatca
     3901 accgccagtt tttccagtcg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc
     3961 cgtttgaagc cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta
     4021 atgtcagaaa gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata
     4081 ttgaacactg ccgtaagaat ttgctcgacc tgcttcgcaa tatctaccac cgtgtcgttc
     4141 agttcggcac gcactttctc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca
     4201 ccattggcgt cgatcagttg atccacaccg caggagatac agtcgtcgat cagctccagc
     4261 actttgccat acgggttgaa gtacagtccc agcttggctt tgttcggtaa cttttcatgt
     4321 aaatacttga tcggcgaggg aatatttagc agcagtagac ggcgaagacc attccacatt
     4381 gcctgttttt gctccagcgg gttatcaaac agtttgatcg ccacgctgtc acgttcatcc
     4441 accagcgccg gccacgcctt cactttatag ttgccacgct tctgttcgta actttccggc
     4501 agctgaccaa aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgcg
     4561 gacagcgttt cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct
     4621 tcttttagct tcttgttttt gtcatccacc acgcggaagg tgattttcag gtgatcgggc
     4681 acctgatccc agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg
     4741 cgctcaaggc tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg
     4801 gcgtagtttg gtgcaggcac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga
     4861 gcaatcacca attcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc
     4921 tggttaagta acggcagcgg aatatgtacg gtcacaccgt ccgcgtctgc gcctggctca
     4981 aactgatagc tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc
     5041 ttgctgattt tttccgcccc ttctttgatc aacatgctct tttcaaagtt gagcaaatca
     5101 ggcgtttcgc ggctgacttt tttccaccag ctatcgaagt gacgagcgga gattacatcg
     5161 tagctgatgc gctggtcgta gaactcaaac agcgtttcgt catcaaccag aatatcgcgg
     5221 cgacgtgatt tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag
     5281 aatgcgtgac gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacga
     5341 cataacgccg gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca
     5401 taaacagtga ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgcggttcg
     5461 ctgtaggtac gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc
     5521 gcagcaatgc gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc
     5581 ggcggttttt tgaataaacc agaaccgggg aagatggaga aacgcgcgtt acgtgcgccg
     5641 gtatattctt gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac
     5701 gcaatgtgaa tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc
     5761 accacctgac gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga
     5821 taatcggtac gacacaggcg acggaaggcg tttgaagaaa gcgccttttg ctgctcgcca
     5881 agataattcc acagatttac aaacgcgagg aagtcggatt ctttgtcgtg gaagcgacga
     5941 tgtttttcgt cggatgcctg ctgtttgtcc atcggccgct cacgcgggtc ctgaatggag
     6001 agcgcggacg tgataatcat cgcctcacgc acgcagccat gtttctgcgc ttccagcacc
     6061 atacgcgcca gacgaggatc gaccggcagt tgcgagagct ggcgaccgag cggcgtcagt
     6121 ttataggcgc tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg
     6181 ccatcctgga tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc
     6241 agccccagcg cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga
     6301 tcggtaaact ccgggcgcga gaggaaatcg tcttcggaat agagacgaat acagatccct
     6361 tcggacacac gaccacaacg gcctttacgc tgattggcag acgcctgtga aatgggttca
     6421 atcggcaaac gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc cgtaccgggg
     6481 tcgataacgt atttaatccc cggtacagtc agcgaggttt ccgcgacgtt ggtcgccagc
     6541 acaatgcgtc gcccgctgtg cgactggaac acgcggttct gttcgctatt cgaaagccgc
     6601 gcataaagcg gcaagatttc ggtatggcgt aagttcagct tgttcagcgc atcggcggta
     6661 tcgcgaattt cccgctcgcc gctcataaag atcagaatgt cgccagggct ttcctggctc
     6721 agttcgtcta cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc atcggcttct
     6781 tcaacaatcg ggcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc
     6841 ggcgcattat taaagtggcg agaaaagcgt tccgggtcga tagtcgcgga agtgataatg
     6901 atttttaggt caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata
     6961 ttcaggctgc gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg
     7021 tcttgctgga tctccgtcag caggataccg tcggtcatca gcttgaccat cgtgttatca
     7081 cttacgtgat cgctgaaacg cactttgtaa ccgatgcaac cgcctggctc cgttttcagc
     7141 tcttccgcaa tacggttcgc caccgttctt gccgccagac gacgcggctg ggtatggccg
     7201 atcagtcctt taatcccgcg ccccagctcc atacagattt tcggtagctg ggtcgtttta
     7261 ccggaaccgg tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaata
     7321 tcctgttttt tctgactaac tggcaaattg tccggataag tgatttccgg tcgtgccgct
     7381 tcacgcagca ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg
     7441 gcctgttgtg catcaggatt tttaaccttc ttaacaccat gcagacggcg agaaaaacac
     7501 agtctgtcgc gcagcatcaa cgaatccagc cgctgctgca aagcagtaaa ggtcaatttt
     7561 tgttgttctg tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga
     7621 tagatataga gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc
     7681 ttcgatttat tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg
     7741 gcatcgccaa ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc
     7801 cagcatcctg gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg
     7861 gcgcgaaaag cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc
     7921 ggtactggat ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc tgactccgcg
     7981 tcagcaggaa gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt
     8041 tatcgttatt gcggcaccga tgtataactt caacatctca actcagttga aaaactattt
     8101 tgacctggtt gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct
     8161 ggtaacgggt aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc
     8221 aacggacctg gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt
     8281 gaaatttgtc ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc
     8341 tgacgcgaaa gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg
     8401 gactgtagtt cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc
     8461 tttgtgatct tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc
     8521 atcaacggtt ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactacg
     8581 cgataatccc aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag
     8641 catcgccacg gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac
     8701 acagaaatag agtcgatctt ttgtcgctcg tccccgaggg aattcaaagg tgacgtccag
     8761 aaccgcattc tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtaat
     8821 tttgctcacc ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg
     8881 ccagggcaat gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt
     8941 tgttatcgcg ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca
     9001 acataaccag actggccacc ataactgaat catcaccatt agctccatca acacaatgca
     9061 cgagcccgcg cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg
     9121 ataattccaa cgacgatcga cgggcaccat ccagtcaatc aacataccta ccgccagccc
     9181 tgtgatgaca tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag cgatgagtaa
     9241 aaaccatccg ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca
     9301 aagtagccag cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag
     9361 ttcaagttgc gaaaatggcc atcccgtcac cccactcact tcaggacgga taaaactaaa
     9421 cttcagcgga tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg
     9481 gacaagtcgg cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag
     9541 atccagactc cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc
     9601 gaatacctga ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag
     9661 agatccatag gtgaagaaaa aaaacggggc cagcaacaat aaccagccag cgccttgtag
     9721 caatacgttc cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc
     9781 actcatcaat ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct
     9841 ccccttgcga acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc
     9901 cggcaatcat ctccagttga gggtgccacg gctgcccggt gtagatgaga atgccgaccg
     9961 gttcgatggc attggcaaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa
    10021 gctcatacag tccagagaca attgccagcg taggccgcgg agttaacgcg ctgagttcct
    10081 ccgggttaaa cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa
    10141 tcatctcttg cccctgtgca acatttaact cgctgtaatc acgtaacaaa atatcgctta
    10201 cggcaggctc gtttgccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa
    10261 ccacgcggac ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagtattt
    10321 gtaaatgggt tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt
    10381 cgattaagcg cccgaatgcg ttactacctt gcggttgatt ttgatagaca taatccagcg
    10441 aactgcctga atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgtcccga
    10501 acaatttcat cgcctttcgc ataaagcgat acgctaaatc aaccggcgat aatagcacgg
    10561 gtccaccaga aagtagccgc cattgatccg ctgatggtcc tgtgcagtct tcatgctgat
    10621 aatcaaattt ttgcgatttg ttagcatata aacgactaat aaagctttgc attttttcaa
    10681 atgccagcgc cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca
    10741 gctctttcag agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca
    10801 catagtcatc acctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatctctaa
    10861 taatacgttc agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga
    10921 tcagcggatc attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt
    10981 tcacatagga attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac
    11041 gtgccagcgg cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa
    11101 ttgcaggcgc ataatcatga acctatgtgg caaccagcac tgcgccgacg ctttgcgcga
    11161 tcacaaccac ctcttccagt ccaacctagc tgtcgctggc agcaaaacgg acaaattcat
    11221 cgacatcctg cactgaacgc gcaagagatg ggctataacc acgcggcccc gaagtttttc
    11281 catgcccccg agcatcccat gcataaaaag cagtatctgg catcgccagt tcatcaacga
    11341 tatgttgtag acgaccagaa tgttcatggc cacgatgaaa taagacaatg acctttttcg
    11401 cccct
//
LOCUS       DADLTD010000139        11229 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLTD010000139 DADLTD010000000
VERSION     DADLTD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.139"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            55..654
                     /locus_tag="JN701_002901"
     CDS             55..654
                     /locus_tag="JN701_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7365285.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR"
     gene            complement(683..1171)
                     /locus_tag="JN701_002902"
     CDS             complement(683..1171)
                     /locus_tag="JN701_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365286.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1184..3991)
                     /locus_tag="JN701_002903"
     CDS             complement(1184..3991)
                     /locus_tag="JN701_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7365287.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQPIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLLETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            complement(4029..4133)
                     /locus_tag="JN701_002904"
     CDS             complement(4029..4133)
                     /locus_tag="JN701_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAY7365288.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            complement(4142..4516)
                     /locus_tag="JN701_002905"
     CDS             complement(4142..4516)
                     /locus_tag="JN701_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365289.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4572..5082)
                     /locus_tag="JN701_002906"
                     /pseudo
     CDS             complement(4572..5082)
                     /locus_tag="JN701_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(5082..6266)
                     /locus_tag="JN701_002907"
     CDS             complement(5082..6266)
                     /locus_tag="JN701_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HAY7365290.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6424..7533
                     /locus_tag="JN701_002908"
                     /pseudo
     CDS             6424..7533
                     /locus_tag="JN701_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            7782..10901
                     /locus_tag="JN701_002909"
     CDS             7782..10901
                     /locus_tag="JN701_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365291.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSIKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSNKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
     gene            11037..>11229
                     /locus_tag="JN701_002910"
     CDS             11037..>11229
                     /locus_tag="JN701_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HAY7365292.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSSEGI"
BASE COUNT         3074 a         2816 c         2514 g         2825 t
ORIGIN      
        1 tggccgtttc tggcctgcat ggcatgattt gcgcttttgg acgggagatt cagtgtgctg
       61 attggctatg taagggtatc aacaaatgac cagaatacag acctgcaacg aaacgctctt
      121 gtttgtgcag gatgtgaaca aatatttgaa gataaattaa gcgggacaaa gacagaccga
      181 ccgggattaa aacgcgcttt aaagcgcctt caaaaaggtg acacgctggt tgtctggaaa
      241 ctggatcgcc tcgggcgaag catgaaacat ctgatttctc tcgtcgggga actacgggag
      301 cgagggatta attttcgcag tctgaccgac agcatagaca catcttctcc aatggggcgt
      361 tttttcttcc acgtgatggg tgccctggct gaaatggaac gtgaattaat tgttgaacgt
      421 acactggccg ggctggcggc agcgcgcgca agggggcgca caggcggacg tcgaccgaag
      481 ctgacaaaag aacagcatga gcaaatagca aggctaatca aaaacgggca cgacagaaaa
      541 caactggcaa taatttacgg cattggcaca tcgacgattt atcgttatca ccctgtaggc
      601 gatatacaga ctgaagaaac aaccgggcag actcaggaaa atgaaaaccg ctaatctgac
      661 cattagcggt ttttgtgtta aatcagaaca gccctttaac tgaactggcc gcgctgttaa
      721 gagatgatgt caccttatct ttgaagccgg acagcatatc actgaacgat gaggattgca
      781 ggcgctcccg caaatcctca tcacagcgtt caagagtcag tgaaaattct atctttttcg
      841 ccttaccgta gcgatcaaac tcggagcggg tcgtattcgt ttcagtcagg acatacatgc
      901 cgtaaatctg cccgacgcca tcaatcaaag gccagggtcg tcctgtatac gcctgcgtag
      961 tcagcagcga cagcgacact tcgccacctg taatttcagg ataaagcaca ccagaaagaa
     1021 cgatgcgatc atcacctgca ccgatatact gccagcttgc tgaacggtta acgcgttcat
     1081 ttttcacatg ccgccagctt ttgttttgct gtaactgctg atgcggcagc gtgcgcagct
     1141 caaaaacaaa catgccgtag atcatcatca tggccatgac tcctcaatct ttatcgtaaa
     1201 aacagccacg cccggcacgg gcgcgccgtt ccatttctgc cctgaccatt tcaccgacca
     1261 gtttcgccag ttcgcgggga ttctgcgtaa caacgttatg cagatgaaca tgaatttcac
     1321 caccaaatcc gaaggcaaca ggctcccggt tacgggaagt tacaggaact gatgccactg
     1381 gagatcgtat agcctccgcc accgggcggg agctggccgc aacaacaggg accagcgccg
     1441 gaggcagcgg agccggaacc acgggtgtga tattaattgc gggggcaggc ttactgacct
     1501 gcgcaatctt ccgctcctgc cactccccac gaacagcaag tgctcggggc aggtttttaa
     1561 agacaatatc gccggggcca atgcgttttt tcgtctcctc aaccagctta cctgtgttat
     1621 cagcaatttt gctgagtctg cgcagcgtac cggtattgct gtctgtgagc ggtttattgt
     1681 ctttggggtt atcacctccg gtgccattgc cattttccac aggcttcggc ggattgattt
     1741 tcgccagatc cccctgaagc aaggcaacct tgtcctgaag aatggccgca cgctgtgcgt
     1801 cttcgatttt cttgcgcgcc ctttccgctt catccggaag cacaccaagc ttttcaagta
     1861 tccacgccag cgtatccagc aacatttttg caggtgtcag aacaagttgt aacgcaccgc
     1921 caagaacgtt accgaatatc tcgccagcac tggtacattt atccagagtt tccttgctgg
     1981 actccatcgg tgacagcagc gatttaaacc agttaaacac ctggctgatc ccactcccga
     2041 ttgcgtcaaa aacaggacca aaccgttcaa aggtttcgag caacggggtc agcctttcca
     2101 taatcccgct gaacaccccg gcaaaaaatg ccctgatggg atcccagtat ttccagataa
     2161 gaacggcagc tccggcaagc gcagccacga taagaccaac cggactgaac aacgccccga
     2221 tagcgcctcc cagtaaagaa acggaacccg tcaccattcc ccatagtgct ggcagaaccc
     2281 tgacagcatt cattgatccg gtcaggaggg aaaaaccaag acgcagtttt gccagcgggc
     2341 cagcaagcac accaatagcc agcgacaacg agccaaccgt tgcagtcatt gccagcaatg
     2401 caccgcctgc aatcagtagc tggcgcgtca gtgcgggatg ggcctgcgcc agcgccgtca
     2461 cctttgatac cacacgcgtg agccactgcg tgacagaacg cagcggaccg tcaatcagat
     2521 ctgcaatgcg gatgcgcaac ccttcccatg cactgctgag tgatttcaga tcgccgtcaa
     2581 ggttgttggc cataaccttt gccgtgcgtt cagcctcacc gcgcgcgcct tcaagttctt
     2641 ttctcagttt gggtaaggag ccgtcacctg ccgcatcaac gagggccata aatgatgtga
     2701 aagcctcttc tccggcaatg tccttaaaga acgatacccg gtcaacttcc ccgtatttgc
     2761 gggtagcttt ataaaggtca gccagcacat cctccatcgg gcgcattttg cccccggcat
     2821 ccgagacaga cacgccaagc tctttcagcg cctctgctgc cgcctttggc ggtgatgcca
     2881 gacgagccag gctggcacgc attgccgtac cagcatcact ccccctgata cccatattcg
     2941 ccagcacgcc cgccatcgct gcggcctgct ccagagatat tcccagctta cccgccaccg
     3001 gacctgcata tttcatggtt tcacccagtg cgcgaaggtc agtgttggta cgggtaaacg
     3061 ctgcggtgag tgtgtcgccg acccggtcca tctggtcagc agaaagaccg aactgcgtca
     3121 gaatatttga gccaatatcc gccgtctcac cgaggtccat accgccagcc gttgccatgc
     3181 tcagtacgcc agggagcgca gcctgaatgg cctgtggtgt aaagccagcc attgcaagaa
     3241 atgcctgtcc actggcggca tcgcctgcgg tgaactgcgt ttcagagcca agttttaacg
     3301 cctgctcacg cagcgcctta aactgcgggc tgttctggtc gattcgcgtc agtgcctgaa
     3361 cgcgggacat ctctttgccg aacccgatcg caggctgcaa aaaacgcccg gcagcatagc
     3421 cgcccgccgc tgccgcacca attgccagcg caccacctgt tttcagtttt cccgctgttt
     3481 cctgcgcgcg cgaataccgc tcacgcgccc gtgttacacg cgcaagcgcc tgccgttcgc
     3541 gttcaagctg gttgttgtac tgttcggtgc gtctgatagg ctgctggatg gtgttatcgc
     3601 tgcctgtcag ggaaatgccg tggcgtttca gctctccgcc aagctcccgc attttctgaa
     3661 tttcccgtgt gcgcgattca ttcaggcgtt caagccgggt gcttaactgc tgcatcagct
     3721 tttgttgttt ttcgctgagc actgtacccg tgcgttgtaa ctgattaagg gcgttaagct
     3781 ggcgtcgtgc tttcacgatg ccagcatccg ctttactgac agcgtcacgg gcgcgctcaa
     3841 atgatcgcgc ctgacgctcg agatttttga tcgccccctg cgttcgctgg atggagtcac
     3901 caaactgccc catcaggcgg cgggcgtttt cggcaggccg ggtcagcctg tcaacggcgc
     3961 tgaaagcgac ccggatatca agagtcttca ttgtctgcat tcccgctacg aagtgccgcc
     4021 cgctcacgtc agctaaccac ttcgccgggc gtcatcatga agatttcggc gggcgaccag
     4081 ttaaaaatgg cggcaatatc cgccaccaga tcttcgatgt gctcaaagca caccagggtg
     4141 attacgctgc cgtctcctgc acgctcttcg cgccagagtc tggctcgctc ataaaattta
     4201 cagccacagc gcacaactga ataaaatcgc gtgacgacat ttttttaatc atcacttcat
     4261 ccagtcgtgg cgaggtcacg cgaggcaaca gcgtgaacat ggtatccgct ttcagattca
     4321 gcacatcaga cagcgacaga ccacgcaggg atccagcctg ctcaatagcc ccggtgatct
     4381 ccacatacgt gattttttcg ccaccacgct caattggtcg ggtcagtttt acgccacgtt
     4441 cgacagccat atcctcaact gccgtcacat tatccgccac ggtgttattc cgggtttcag
     4501 tatcgatgtc tttcatctgt tgtctccttt tcagtcagag gcgacgcact gcgccgccag
     4561 catattactt atcagccaag cccaagcgcg gaacggatgc gatcgggcac aatgtccttg
     4621 ccgtccttcc ggtaaatgaa gttcagcagg tcaatctccc acaacgggcg atcgttaaca
     4681 ctcagcttgt agtaggtgtt ttaatggcgt aagtgtgtga tgtggcttcg ccctgtttgg
     4741 cttcccccat atcaatttcc gtcacacgtc cgcgcatttc gacttcatac aggtcgcttt
     4801 ctgcatcggt gtagtattca cccgcaaaac gcagcagcgt gccgtcaatc gtgccgccat
     4861 acttaaggaa cagctcacga actgcgcccc catgacaaag ctcgcatcaa gcgcggagtc
     4921 gtccagaccg agatcaatac ttaccgcacc catcatgcca ccaccccggt agctgtcggt
     4981 tttgcgcgtc agcttaggca gagtgacgga cgtcacatta cccacttcgt tttcaccatc
     5041 cacaaacagc gtaaaaaagc gaagatgttt tggcacagcc atcaggcacc tcccagcacc
     5101 gcaaatgcgg gttcaaagta ttcatcagta aacgtctggt aaagctccat gtcttccagt
     5161 ggcggaacgg gcgtatattt gtagcgaata cgcacacgtc cctgacgtaa atccgtggtg
     5221 ctgttatcca ccacgtcata ccagcactcc gcgccaatca gtttcccggc agtcaccagt
     5281 gaatccagtt ttgccctgat ggcactgata acatccttca cattcgcagg cgtcagtgga
     5341 ctgtcgatgg tttcaaactg cgcttccgca attgaatcag ccagcacctg tgcggttcgg
     5401 gtatacacct caaagatgta ggcgttcgtt tccggtgtgc ggttacccca gaagcggaac
     5461 ccgttgcgac gaataatggt cgtgatttct ttgttgttga ggctgttggc atcgctgtct
     5521 tcggcctgca acgaccagaa cacatgcctc gacatcccca gcacattttt aaccggaacg
     5581 ttggacagcg atttgtgcca tccctgttca tggtcaatgt acgcacgaag gccgcacgca
     5641 taggcaggcg cggggaacgt ttcgtttttg ccatttttcg ggttgtaggc gatgaagtcc
     5701 ggccataaga gcatcacctc acgttcgttg aatttctggc ggtaggtaat tgcctcggcc
     5761 atcgtgttac agccatgaca tgaggcatac acaaacgcgc gcagtttatc tgcaatcacg
     5821 cacagggatt ttgttaccgc ctccgtgtcc agctccggcg cggccagaat acgcggacgg
     5881 tatccgatgc tttcatcctg ctctgcaaca agcagcgcat acatccccgt atagctgccg
     5941 tcatcctcag aaccaccgat aaccagttga tcctgcgtct ttccgtcttc ttctttgtgt
     6001 tcagccacgc gaacgacgat cacctttgtg ctcacctggt ctgcgatggc cttaagcgca
     6061 cgataaagcg tccccgttgt cccgcatttt cccagcacgt cattgacgcg ggtcagcagt
     6121 gtgggcttgt tcagcgggaa cagcttcgcg tccgcatcat ccgccgttgc cacgataccg
     6181 ataacgctgg aatcaacatc gttaatcgct gttaccaggt cggtattctc cgtaacacgg
     6241 gcaccatgaa aacgagtttc actcatagct tcagcccctt gtatccgtta aatgattcag
     6301 caacaatcat cacccaccac gcgcgtaatc tcacccctgc gccgttctcc cgccacggcg
     6361 acaacaaaaa gcagtaaccc cctccgcacg cacatgcgac catgccgcac agggagggag
     6421 cagatgaccg acaccaccat gcaattgctc agtcagggca cggcccccgt gaaaatgccg
     6481 gattttgata ttctctcgga gggtaaaacg ctgtcaggcg tggcggagcg cctgatgagc
     6541 ctgtaactga ccgacaaccg gggatttgag gcggaccagc tcaccatcac gctggatgat
     6601 gcggatggtc agttgcagct accgccacgg ggcgcgcgtc tgacggttct cattggctgg
     6661 aaaggggaac cgctgacaga aaaaggcact tacattgttg atgaaatcgc tcacgaagga
     6721 ccgccggaca ggctgactgt ttcagccaga agcgcagatt ttcgggatga atttaacgtt
     6781 aaacgtgagg tgtcctggca tgatgtgacc gttgagcgtg tggtatccgc catcactcat
     6841 cggtacggtc tgaaaccgca aatcagcgaa atgctgatgg atatcgaaat cgaccacgcc
     6901 gaccagaccg aagaaagcga catgtccttt cttacgcgca tggcggaaat gctgggagca
     6961 atcgccacgg taaaaagcgg caatctgtta ttcatcatgc caggtggtgg cgtgaacgca
     7021 caggggcagc cgttgccctc gttcgccatt acacgcagca gcggcgatcg ccatcagttc
     7081 cgcattgctg accgcgaggc gtatacgggg gtacgcgcct actggcttga tcttaattac
     7141 gggaaaaaga aaaaagtcag cgtgaaacgc cgcaaaccgc caaaacccaa aaaggagaaa
     7201 agcagcagtc gggaaggcga ctatatggaa ggtgcggaag gcaacgtgtt tgtgttacgc
     7261 aagacttatc agaacgagca ggcagcaaga cgcgcagcgg cggcaaagtg gcagcagcta
     7321 caacgcggag ccgcatcatt ctccatcacg ctggcgcgtg gtcgtgcaga actctatccc
     7381 gaaatgcatg gcacagtgac aggctttaaa agcgagattg attatcagga ctggactatt
     7441 gcaaaagccg agcacaccat tgataacagc ggctttacca cgcagcttga acttgaagca
     7501 aaaatcccgg aatggatagc agaaacagaa taaatctctt aaaaatatta tttttgagat
     7561 gtgcttctac actgacagta agaacaaaag ataatgtatc agcccactag atgtcagagg
     7621 ttggagcatc aaccagatgc taacaataat acggatgttt ttcccacatg acagaggaag
     7681 tcaagtataa tcttcacagt ccatcccgtc caagggaata tgactcaata gtaaaagttc
     7741 cagtttagtt agtagaccca cactttatag aagagtaatt catgattgga caatttttaa
     7801 gtgcaacaga gattttagca aagaactatg ttcgtaataa aatggttaaa aatccatttt
     7861 attcgaattt aaaatggaat tttattgaaa aaaacatcat aagattaaca tcctcaccgg
     7921 ttaaatcagt actttgcata tcagcttttt catttgtact actttatgtt ggatatttaa
     7981 acgaattatt tataaaaaat aatcttttac attatttccc gtttaggcat tcattaaccg
     8041 aatggcaaac aaccatttta agtggccaat tgacaattat tggtattgta tacccccttg
     8101 ttataggttt agtaagtgtc ttattccaaa aaaaggcaga tagaaaaata gcccaaactg
     8161 cttatcagcg ttactcagga ttcatgcttg ctggactcag cgggcttttc ttatcaggat
     8221 ttatacttct cagtgtacta attaaaactg tctttggaag ttatctttat ggtatagctt
     8281 gtctaatcag catattatgg cttttgataa acatagttct ttctatttgg ttctttatcg
     8341 tgagtcttga aattcttgat gatgtaaaac ggcaaataat tataaagcga tacattgcct
     8401 tcgaaatagt aatgccccat atttgtaaca aaatctcagc caagcttagg ttatacccta
     8461 tctatcaaaa acataactat tcaaatttag aaatcacgca agccgattac aaaggagaat
     8521 acatatctgt ggctagcagt tattctaagg aagatgagtt aagtttatat caccgtcctt
     8581 ttcaacttac tcttaatctt attaattacc aactaaaaaa gaagaatcat tttgcgtcat
     8641 ttgtcattgg tgataatcgt acaaaagaaa cagaatccac gggtaagata ctttttagca
     8701 ttaaaaatat caaaccagac agcttattaa taaaaatact taagcaatgt ttttataggg
     8761 ctccgattaa aggaggagat ttttccgtaa gtcttacaat gcaggctata acagcagata
     8821 cgtatatgta tctgcgggat tctgatctca ttagttttga caatgctatc tctgcattaa
     8881 ttaataactt taataatctc tgcgatttgt atttttttca agatgataat accaacaata
     8941 acttcttact gatcactaca gaattatttg aacggagttt tcaatatgag ttttcagatg
     9001 aagtttataa aatatcaaat aattccatgg ataaaattaa tctttctgaa agattttttg
     9061 aattatgtct ttggagcgga gtgcgtatta tcaataatag aaaacatctt atcagcaatg
     9121 aactttgtat ctatatggga attactcgtt cacaatggtc aatattaaca gaatggttcc
     9181 gaaacaacca atcactctta aatgcctcgc ttcgttcacg ttataatcgt atattaagaa
     9241 cctatatcac tgtttgggag caatatcaag aaagcataaa ctttcgtttc tgcaatacag
     9301 agaattcaga tttatttgaa ttattttgca aaacgcaact acaagaactt ccatcgatga
     9361 ttattgatgc aacacagact cgcgatcctt ctactattga tacagccgtc gatcttatta
     9421 accgatggca gcactcaatg aatattgaca gccattcggt agaaaaatac agttacaaag
     9481 gccagctttt taacccgggt tttttcatat ccaaaaaact aaacttcaat tcagacagag
     9541 agtggttcaa tattgccatc ataaatgcgt tgactgacat gcgtatatgc acatgtcttt
     9601 atctgacatc aagaatcaat acttctgaca aactaatgac acattacatt aaacttattt
     9661 tagagggtaa acttattgac caaaccggtg gatatgaaac tcccactgaa gaaattgata
     9721 atgcaagcca actaatcaag atattaatta gaatctgcct gtggacatgg tctgaaaata
     9781 tggagcacaa tgggtggatg aatagtttag caaggcgttt acgtgactat gataaaacag
     9841 acatggtgat gggcagagtt tacagtaatg tttttgactg tggatttatt gatatggaac
     9901 aatcatgggt tcagctatta ttaatttttt ctaacaagaa cgatagcgta tcaaaagaaa
     9961 taaaagaagc aatagaaaac aactatataa catatcgtga gaagcaacga ctcattggag
    10021 tattatcaaa aatttgtaac tccatcgaat acactaaaat caagctgact ttaacattgg
    10081 acgatctcca aacaaaaaaa gaaaacttaa gaaagttgct tcaagagcat attaacatgc
    10141 taaagaaaga tcttgatatg agacttcagg atgctgccat agatgtacat cgactagata
    10201 gcactgcacg taaaacatca gaacatcttc gtaaacgcat caagaaaacg ctccctcttt
    10261 cacttttcaa atctattgat tttaagcagg ctagcgattg tttcacaaaa cataagatat
    10321 ctattaagat agataaagaa ccgtatgccg aaggaataga atccatccct tatatcaatg
    10381 aaggtgatat tcaagcagac ttaattctca aggacatcca gagaataata ctttcaaatt
    10441 tatttagcac tggctgttcg caacataccg taatagaaga tttcaatatg ttgattgatc
    10501 acataaaatc atctgctgac ttggctggca aactggttct tgtgatgagt aaagagatct
    10561 ttcagcaata taatcgtatg cttttcgata atcccaacct cagagaacta atgagaaaaa
    10621 atgatgatgg ctctatgaac ataactactg aatccggtac ccgtaaggtt tacttcttac
    10681 catttgttaa tcaaccattt tctcttgtgg taaaggataa ctacttcact aaattaatta
    10741 ttagagaata tgataataat aagttagtaa atgtcacttc agagaatatc aaaagtgact
    10801 cagataaatt caagctaact cttaactatg agttgaatat agtatttgaa ggaaacgctg
    10861 atttaaaaat agctcactcg caaagagtga catcagaata atatctttga cggcgctcct
    10921 tagagtgccg ttcgtttatt aactaatgct aaaaatcatt atatagagaa agtcatgttt
    10981 aaatcttaga gaatacaacc tagaatagtg taagcactac aataagagag gtcgctatgt
    11041 tccgttgtcc gctttgtggc gcatctgccc gtatccgcac cagtcgtccg gaaaatgatt
    11101 caaacaccgt gagacaaaag tattatcagt gtaacaatct agaatgcggc gtatgtttct
    11161 caacactgga agccttccac aaattcactt caaagcacgc ctcccccatt cactcatcag
    11221 agggcatcc
//
LOCUS       DADLTD010000140        11212 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLTD010000140 DADLTD010000000
VERSION     DADLTD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.140"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..868)
                     /locus_tag="JN701_002911"
                     /pseudo
     CDS             complement(1..868)
                     /locus_tag="JN701_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(843..1643)
                     /gene="otsB"
                     /locus_tag="JN701_002912"
                     /pseudo
     CDS             complement(843..1643)
                     /gene="otsB"
                     /locus_tag="JN701_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1810..2799)
                     /locus_tag="JN701_002913"
     CDS             complement(1810..2799)
                     /locus_tag="JN701_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY7365293.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVMFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2811..4325)
                     /locus_tag="JN701_002914"
     CDS             complement(2811..4325)
                     /locus_tag="JN701_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY7365294.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4395..5383)
                     /locus_tag="JN701_002915"
                     /pseudo
     CDS             complement(4395..5383)
                     /locus_tag="JN701_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
     gene            6097..6600
                     /locus_tag="JN701_002916"
     CDS             6097..6600
                     /locus_tag="JN701_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY7365295.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6679..6930)
                     /locus_tag="JN701_002917"
     CDS             complement(6679..6930)
                     /locus_tag="JN701_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY7365296.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7045..7131)
                     /gene="azuC"
                     /locus_tag="JN701_002918"
     CDS             complement(7045..7131)
                     /gene="azuC"
                     /locus_tag="JN701_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY7365297.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7394..7717
                     /locus_tag="JN701_002919"
     CDS             7394..7717
                     /locus_tag="JN701_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365298.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7888..8385
                     /gene="ftnA"
                     /locus_tag="JN701_002920"
     CDS             7888..8385
                     /gene="ftnA"
                     /locus_tag="JN701_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY7365299.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8423..8662)
                     /locus_tag="JN701_002921"
     CDS             complement(8423..8662)
                     /locus_tag="JN701_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY7365300.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8853..10064
                     /gene="tyrP"
                     /locus_tag="JN701_002922"
     CDS             8853..10064
                     /gene="tyrP"
                     /locus_tag="JN701_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY7365301.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(10126..10791)
                     /gene="yecA"
                     /locus_tag="JN701_002923"
     CDS             complement(10126..10791)
                     /gene="yecA"
                     /locus_tag="JN701_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY7365302.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10987..11073)
                     /locus_tag="JN701_002924"
     tRNA            complement(10987..11073)
                     /locus_tag="JN701_002924"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11037..11039),aa:Leu,seq:taa)
     gene            complement(11086..11159)
                     /locus_tag="JN701_002925"
     tRNA            complement(11086..11159)
                     /locus_tag="JN701_002925"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11125..11127),aa:Cys,seq:gca)
BASE COUNT         2805 a         2848 c         2594 g         2965 t
ORIGIN      
        1 taacgaattt taccatgatg ctgcggatat ttttccagca acgcttcata ggcgagaaaa
       61 cgctctggca aacctttgga ataatccagc cgttcgacag aaaagatatt ttgtacgttt
      121 ttcagttccg ctttaagttg cgccagtttt ggcggcagtg gcccggcagc ctgtttggct
      181 atttctttcg gttcaatgcc gatcgggtag acttctgttc gaaatgcttt gccccaggct
      241 gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt tagaaagaca atccaggaac
      301 gccagacgat cgttttctgt ctggaaaccc agcaaatcat aatcacaaag ctgttcaagc
      361 aaggtgtcat atgtcggcag cgcgttgaag atttccggtg tcgggaaagg aatatgcaga
      421 aagaaaccaa tgcgattatt caccccacgt ttgcgtaatt catgcgcaaa gggcaacagg
      481 tgataatcgt ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc
      541 agcaacgcat ttacgcgtag atagccgtcc caggcaggac gctgaaattg catcagatcg
      601 agccgataat gaaaagcggg ccagagaacg gcattggaga attggttgta gtattcgtca
      661 aggtcctgtt cgctgaggtt aaaagaggcc cacgtaatgt tacctttttt agcggctgat
      721 cctcattccc tgtttcacca ctccagccaa accacagtcc gcctgtggct ttcagtgccc
      781 ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc
      841 ggttagatac tacgactaaa cgactcatag tcatcactcc tgttattttc tcttttttgt
      901 tgtaatgcgg tggttatcat ttcaagcctg ctccagacat ccggcacacc cgccagtcgc
      961 catgatgcct gagttgcacc tgtgccaatt tttactgaca ttccgcccag tcggttaacg
     1021 actgcgaagc cagattcatc ggttaaatca tcgcccagaa atacgggcgt tcgcccgata
     1081 aagggagctt cctgcataaa agctgcaatt gcctcacctt tactggtacc tctcggtttg
     1141 atctcgacaa cacactttcc ctgctgtaac gccatttgtg gccagatctg agtaatacgt
     1201 tacgctaatg tcattaatgc gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa
     1261 gccatccctt tcgcctccag ctccgcgccg ggatactgag cgataactgt atgcagttgc
     1321 acgctaatat cacgcgcaat cgcatccggc agatgaacga tatgcgtttt accattgatg
     1381 tcacggcgct ccgccccatg cacgcccgct aacgggaagc gataaggttt tgccagtgcg
     1441 tcaagctcca ccattgagcg ccctgatatc aatgccaatg caccatcact tgcggttgcc
     1501 agtagctgta gtccttgcag aatattgtca ggcacgacga cctgatcggg atgcggtttg
     1561 atttccgcca gcgttccatc gagatcaaaa aaccaggcat atttcgcgga tagttcaggg
     1621 gtttcggtta acggttctgt cacccggttc tcctacttct cttttcttat tgttttgcgc
     1681 agaacgcgct ttacagacat gtaagtatag acagtgtgac gggggtcgcc atttgcaacg
     1741 aagaacagcc aaccgttatg gcggttggct gtggtgggaa aaaacgctaa attgttgcag
     1801 aaaaaagcat cagacagtgc gtttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc
     1861 cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa gcaggttcat
     1921 ggcgttttcc acggtgccta aaattaagat acccgccacc acatatgaga tttttccgat
     1981 gcctcctttc agagaaacgc cacctaaaac gcaggcggag ataacaatca gctcgtaacc
     2041 aatcgacgtc attggctgcc cactggtcat acgtgaagcc agaataattc cggctatcgc
     2101 agataccagg ccagagagaa caaagataat aattttggtg cgaacaaccg gtacacccgc
     2161 cagacgcgca gcctcttcgt tcccgccaat cgccagggtg ttacgaccaa aggtggtttt
     2221 attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg gcgcaggcag
     2281 accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac cgaccgcttt
     2341 accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca gtgttgtgat
     2401 cagagcattt attttcagtt tggcgataac aaagccattg accaggccac agagaatgcc
     2461 aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga taaccaccgc
     2521 cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt caccggaagc
     2581 aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca ggcccaaccc
     2641 tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga taaagagcac
     2701 cgcaaacatc accagcatgc cgtactgatc ccagatacgc ccgaagctga atgacgactt
     2761 aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac tcaggcaact
     2821 gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt
     2881 aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc aacgccgagg
     2941 acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc cagcgcataa
     3001 attacgttat atatttcgtg cttagcacca acatcaatgc cgcgcgtagg ttcatccagc
     3061 aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg ctgatttccg
     3121 cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag cgaacgaatg
     3181 tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc gagcacatgt
     3241 ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat aatgccttcc
     3301 gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct cggtttacga
     3361 atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt cccgccaaac
     3421 aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc aacaatttca
     3481 ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac agcatcaaga
     3541 cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc aatgtcgcgc
     3601 ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg
     3661 acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat ttcttccata
     3721 cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat aacgcggaaa
     3781 agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc gataatttta
     3841 gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat ggagagatat
     3901 ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa acccgcctca
     3961 taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc gagatagatg
     4021 ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat cgccacaccc
     4081 gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat cactacagaa
     4141 cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt tcctgcgcca
     4201 ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat atccgtcagc
     4261 gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg ggtagactgt
     4321 tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat gcagagggga
     4381 atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc tttaaagttg
     4441 tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc aacgtctttt
     4501 gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg gcttggcagc
     4561 agggaaccgt agaagccggt tgcctgtact ttagacagtt cgctcaccgc atccacaccg
     4621 ttaatgccaa tgccgataat atcggcagct ttaaagccct gaccttccgt cgcgcgtacg
     4681 ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac ttcaggatgt
     4741 tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga tttggtaggt
     4801 acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat agatcccgta
     4861 gtacggcggc gggcagtatc cagttcgttg gcggtaatca ccatcaccgc gctttctttg
     4921 acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg ttcgccaatt
     4981 ttagtcgcgc catcatcacc agcggaacgg tatccattgg cttacctttg gcgttaacaa
     5041 actgatcatc cacggcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     5101 agccgagttt ggggtccgga gtacaaataa cgaaaccttt tgcgccactg gcagccaggc
     5161 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     5221 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     5281 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     5341 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     5401 gtgtcgtttt gtgtagggca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     5461 attacctttt aactaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     5521 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     5581 ggaaaagaca taagggaaag ccaatttgtc agacaaattg tcgaatgcac agcagattaa
     5641 tccataagat tagcttggaa atccttgttg tctttggtac ccatgcggga tgtcttcttt
     5701 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccgc
     5761 aactcaaaga tgtggaaaat gcacgtcatt catttcgcca ttaattatca ctgtgctcat
     5821 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     5881 atcatcattt catcaatgaa atgtaaaaat atataaactt gatgatttaa gcattttctt
     5941 atacccgttc agacgttatt cttatttcag atcatcgtca gaattgactc cacgatcaca
     6001 tttcggaccg gcagaaagga attattctgc aaacagtaat tatggtgttt tgatttatct
     6061 tgcacctctc cacttctgga tataaggata ttaggtatgg caaccgctgg aatgcttctc
     6121 aaactcaact ctcaaatgaa ccgcgagttt tacgcatcca atctctacct tcacctgagt
     6181 aactggtgtt ctgaacagag tctgaacggc accgccactt tccttcgcgc ccaggcacag
     6241 agtaatgtga cccaaatgat gcgcatgttt aactttatga agagtgtcgg cgctaccccc
     6301 atcgttaaag ccattgatgt tcccggtgaa aaactgaact ctctggaaga actgttccaa
     6361 aaaacgatgg aagaatacga gcaacgttct agtacgttgg cacagttagc cgatgaagcg
     6421 aaagaactga atgatgattc aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag
     6481 catgatggtc tgttgctgca aaccattctt gatgaagtgc gcagtgcgaa acttgctggt
     6541 atgtgcccag tgcagaccga ccaacatgtt ctgaatgtcg tgtcgcatca gctgcattga
     6601 tcatacatcg gcgctaatgc attgcgccga tgaagttttt gagaaaccgc tgcctcatct
     6661 ttttgaagca gcggtttttt aatgggattc gccttgtggg gtaaacttga gttcaatcag
     6721 cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttact
     6781 aaagtcgata ttcttcagct gactggacat tttttcacga acttcaacgg gcgcgattac
     6841 atcgagaaca tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag
     6901 ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg
     6961 ttatagtagc ggcttcacat cttcagcgat agtcacatcc accgtcatca ggacacaaaa
     7021 aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa
     7081 cgtcttgcaa tagttattga acatactttt caggattttg cgcagtttca tcgcggcact
     7141 ccaaccattt gttatacatg tgttattttc tttgtgctta taatatgaca accatcacaa
     7201 aaaccaatct ttatgtgatg caaatcacac aaataccccc ttaatgttat aaaaatgata
     7261 atcaaaaaac agccccccca tttctgacac ctacagatgg caagaaatag cgcctgccag
     7321 gcgtcttttc cggtcattgt cgcagcgctg taacgcgtaa aatagtgctt tcttttactc
     7381 ttctggctgg accatgagac ttctgattct gactctttca ttaataacgc tggcggggtg
     7441 tacggttact cgccaggccc atgtgagcga agttgacgcg gcaacaggta ttgtacggtt
     7501 ggtttatgac caggcttttt tgcagcatgc ccataccgat cgttatgtca gtcgcgggat
     7561 cgccgatcgc gcatgccagc aggaaggcta tacccacgcg atcccctttg gtcagccagt
     7621 aggcaactgt agcctttttg cgggttcctt atgtttgaat acggaattca ctttgtcata
     7681 tcagtgccat cattccgcct tccctgtctt tctctaaata ttttaatcag cgaggggatc
     7741 ttcgctgatt aaaaaaatag cggtaatgcg ttttattccc attcgtattt ttaataattg
     7801 aagtttatat tttacctttt gcaaataata aaataacaaa ttatagtgac gccacagcaa
     7861 caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat
     7921 gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc
     7981 agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca agaagagatg
     8041 acgcatatgc agcatctgtt tgattacctg actgataccg gcaatttacc gcgtattaat
     8101 accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat
     8161 aaacacgaac aattaatcac ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat
     8221 caggactacc caacatttaa tttcctgcaa tggtatgttt ctgagcagca tgaagaagag
     8281 aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg cgaaggtctg
     8341 tattttatcg acaaagaact ctctaccctc gacacacaaa actaatactc tccggcggca
     8401 ggtttactgc cgccgtttca aatcagtgac ggcaaatctt actctgatcg gtcgaaaaac
     8461 cgtctttcga aggtataaat ttgccttttg ctgccagaaa cgctaccagc tctcccgctg
     8521 tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc accaaaacgc gctttaattg
     8581 ctgcttccag actggcatgc gtatattgct cgcctgattc aatcatcata tttaacactt
     8641 catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa gcctagcgta gcgattgccg
     8701 cttatgaaga ctttgcgcca gagcagaact gaatgctttt tattgtacat ttatatttac
     8761 accatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat
     8821 cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaactctg ggaagtgttt
     8881 ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg gctgcggcag
     8941 gtgttggttt tagcgtcacg ttaatcttgt tgattgggct ttgggcgttg atgtgctaca
     9001 cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt ctgggcacgc
     9061 tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt atgatgttct
     9121 taatgtatgc tctgactgcg gcatacatca gcggtgccgg tgaattgttg gcctccagca
     9181 tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg cgtgctgttg ttcacttttg
     9241 ttgccggtgg cgtggtttgt gttggaacat cgctggtcga tttatttaac cgttttctgt
     9301 tcagcgccaa aattattttt ctggtggtga tgctggtatt gctgctaccg catattcaca
     9361 aagtgaatct tttaaccctg ccgttgcaac aggggctggc tctgtcagca atcccggtga
     9421 tttttacctc atttggtttt cacggtagcg tgccgagtat tgtcagctat atggatggca
     9481 acgttcgtaa gctacgcagg gtgtttataa ccggtagtgc gatccccctg gtggcatata
     9541 ttttctggca ggtggcaacg cttggcagca ttgattcaac aacctttatg ggattgctgg
     9601 ctaatcatgc tggattaaac gggctgttac aggcgttacg cgaaatggtg gcctctccgc
     9661 atgttgagct ggcagtgcat ttatttgctg atttagccct cgccacgtca tttctcggcg
     9721 ttgcgttagg cttatttgat tatctggctg atttatttca gcgttcaaat accgttggtg
     9781 gacggttgca aactggtgca attacctttc tgccgccgtt ggcgtttgca ctgttttatc
     9841 cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta ctggcattga
     9901 ttatcccttc gctgttgacc tggcaaagca gaaagcacaa tcctcaggcg ggttaccggg
     9961 tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct gtgattggcg
    10021 tgcaattttt gattgcggca gggttgttac cagaagtgag gtgatcagat agcctcaaat
    10081 tccttattgg gtgccagaat taacgctgac acccaatttg gcctcttaat gcaggcagca
    10141 ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg ttacgccccg gtttctcttc
    10201 tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt acgcatgtag
    10261 atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg gcgacatctt
    10321 ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca gcgctggttt
    10381 taacgaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat agccaaagca
    10441 ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga ataacggctc
    10501 aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt gaaaagccag
    10561 gttcataaag cgcgtcatct ctttctctga cgcccagcgc ggcacatagt cagccccacc
    10621 ccacacggca accagccact gttccggttc aatctcttgc ggagaactca acaccgccgt
    10681 caataaatcg tccagctccg ccacatcaag gatggcgtgg ttagtgttgt atttggtcag
    10741 aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca tacgcttttc
    10801 cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca gagatacttt
    10861 ttgagtggct ttgccggtga ttaaaaatta aggagggtgt aacgacaagt tgcaggcaca
    10921 aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt attcttatct
    10981 ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga gggattttaa
    11041 atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag gcgcgttccg
    11101 gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt taacgcgcca
    11161 aattcttcag gcctttcagc cagacatccg cttgacgccg atgtctttta aa
//
LOCUS       DADLTD010000141        11209 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLTD010000141 DADLTD010000000
VERSION     DADLTD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.141"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..871)
                     /locus_tag="JN701_002926"
     CDS             complement(<1..871)
                     /locus_tag="JN701_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HAY7365303.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLD"
     gene            complement(888..1721)
                     /locus_tag="JN701_002927"
     CDS             complement(888..1721)
                     /locus_tag="JN701_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HAY7365304.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTFMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            1864..3630
                     /locus_tag="JN701_002928"
     CDS             1864..3630
                     /locus_tag="JN701_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HAY7365305.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDDQIEKLEKIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVT
                     VEDAVRGACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            3630..4661
                     /locus_tag="JN701_002929"
                     /pseudo
     CDS             3630..4661
                     /locus_tag="JN701_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520363.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(4768..5649)
                     /locus_tag="JN701_002930"
     CDS             complement(4768..5649)
                     /locus_tag="JN701_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365306.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(5633..7282)
                     /locus_tag="JN701_002931"
     CDS             complement(5633..7282)
                     /locus_tag="JN701_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7365307.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(7575..7808)
                     /locus_tag="JN701_002932"
     CDS             complement(7575..7808)
                     /locus_tag="JN701_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7365308.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(7819..8007)
                     /locus_tag="JN701_002933"
     CDS             complement(7819..8007)
                     /locus_tag="JN701_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365309.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(8161..10575)
                     /locus_tag="JN701_002934"
     CDS             complement(8161..10575)
                     /locus_tag="JN701_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HAY7365310.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(10572..>11209)
                     /locus_tag="JN701_002935"
     CDS             complement(10572..>11209)
                     /locus_tag="JN701_002935"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HAY7365311.1"
                     /translation="RVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYR
                     GLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVV
                     YCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFT
                     HHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         2876 a         2878 c         2479 g         2976 t
ORIGIN      
        1 tatccagacg ggtgatcagc agcgcattcg gcgggaagaa cggcgcacgc acggcctgca
       61 ggccacccat gcgtttctga ctgatgatca tatcggcagc cagtttttca ctgttttcct
      121 gctctttgtt gaccagcggg aaatacttgt cagacagcag ttcacgaccg caaatcacca
      181 ccagatcgtc atcgtcctgg tagaccacgt cgataagctc attgacggca tccatcacca
      241 cggcgtccag gttggcatat tcgccacctt tcccgacttt cactgcgccc ggtgtggttt
      301 caccgcccgt ggtggtgctg cccatgacgt gatccggtgc atcctcacgg attttctgca
      361 gccagccttt attcacatcc tgcagcagcg ggttttcgct acggttggag gttttcgcac
      421 gcttcacgcc attaaagccg atcatgatgc ggtccagtgc ctgacgtttc acgatggcgt
      481 cacggatacg cacctgaaaa tcctgaaact tcgcccacag gtccagcttc gcgtaggtca
      541 gcaccgtgtc aaagttggtc tgctcgcatt tatattccac atcgaccatc agcgtcggat
      601 cgacaggttc acgctctttc gcagtggtat cagtggttcc ggcaatggtg ctgccaactc
      661 ccaaccccag cagctgaccg gactgctcag tcactggcgt gacgttaatc agagtcagga
      721 aagcggcgga ctgctggatc tggtcttcca gcgtctgctg cacggacggc tctacggtga
      781 acttgctgga cagttcttca actgccacac cgttcagacg cgccagttgc tgcaggtaag
      841 cgttaaaagc aaagcgggta ttcttcttca tcaggttttg tgctccatca gcaattggtc
      901 agagtgtcag cgggggcgtt accgcctgtt gcacgctggc ggtagtcctg gcggctgtct
      961 tcatgactca gcttgtccac cagttcgtta aaggcggttt gctgtgcctg cagggcagtc
     1021 tccagctcag acaggcgttc ttcctgctca gacagggatt tttcggtgcg tgcgctcagg
     1081 ttctgctgct cagtggcgac cagctccacg gccttatgca catcagagaa ccgggcgtca
     1141 tcggactgct cttttttggt aaacagcgcc gtgacgcggg caaacaggga cggtttgtcg
     1201 tcctggactt cttccagttc gatcaccgtt tcctctgcgg cggtaaagag attggcggga
     1261 ttctgcttgc ggtttgccag cgggttatgg gctgcactgg cgctgaatgt cagcatttca
     1321 gtgcccagac tggcagggtc atcagtggca gccaggccga ctaggtaggc tttgcccgta
     1381 tcagcgaact tcgggctgac ttccataaag gtgaataatt tctggccttt tttcaccagt
     1441 tccaccaggg actccgttgg ctcaacgtca gcatacagcg ccatcttgcc tgccagcgga
     1501 ccttccgtga tttcttcagc aaacagcgcc gtcaccttgc cgtagcggtt aaaggtgctg
     1561 tccggcagat aagacttgat gtgctcaagg ttaatcagcg cggtatacac cgccgggtta
     1621 tagctggctg ccatctgttc cagccattca cgctggattt cgcgtccgtc ggtggtggca
     1681 ccttccaccc cgatgcgaaa acgctttgct ttcactgtca tgagccgtgc tccgttagaa
     1741 aaaacttact ggagccttat ggttgcggtg ataggggcag tgaaacaatg cgcggtattt
     1801 gtaccgacaa ccacacaaac cgcaggcggg gaaagccttc attcaaggct gtaggtttgt
     1861 gccatgaaca ccacactgac acccgcagat ctcgatcccc gtcggcaggc catgctgctg
     1921 tactttcagg gataccgcgt agcccgcatt gctgaaatgc tgggcgagaa agttgcaacc
     1981 gttcacagct ggaaaaaacg cgacaagtgg ggtgactatg ggcctctgga tcagatgcag
     2041 ctcaccaccg ccgcacgcta ctgccagctc atcatgaagg agcacaaaga agggaaagat
     2101 ttcaaagaga ttgacctgct ggcgcgccag tctgagcgcc acgcgcggat cggcaagttt
     2161 aacaatggcg gcaacgaagc cgacttaaac cctaacgtcg ccaaccgcaa caaaggccca
     2221 cgccgtcagc ctgaaaagaa tgttttcacc gatgaccaga ttgagaagct ggaaaaaatt
     2281 ttccattcct ccatgttcaa ctaccagcgc cactggtggg aagccggaaa aaccaaccgc
     2341 atccgcaacc tgctgaagtc acgccagatc ggcgcgacct tctattttgc ccgtgaagcc
     2401 ctgattgacg ccctgcttac cggacgtaac cagattttcc tttctgccag taaggcacag
     2461 gcccacgtct tcaaacagta catcatcgac tttgccaaag aagtggaggt ggagctgaaa
     2521 ggcgatccga tggtgcttcc caacggggcc acactgtatt tcctcggcac caatgcccgc
     2581 actgcccaga gttatcacgg taacctgtat ctggatgaat atttctggat accgaaattt
     2641 caggagctac gcaaagtggc ttccggtatg gctattcaca agaaatggcg gcagacctat
     2701 ttttccacac catccagtct gacccacagt gcttatccgt tctggtccgg tgcgctgttc
     2761 aaccgtgggc gcaacaaagc tgacaaggtg gacatcgacc tgtcccacag caatctggcc
     2821 cccggccggc tgtgcgcaga cgggcaatac cgccagatag tcaccgtgga agatgcggtg
     2881 cgcggcgcct gtaacctgtt cgaccttgac cagttgcgca tggagtacag ccctgacgaa
     2941 taccagaacc tgctgatgtg cgagttcgtg gacgatctcg cgtccgtgtt cccgctcagc
     3001 gaattgcagg cgtgcatggt ggacagctgg gaagtctgga ccgactttca tgcactggcc
     3061 ctgcgcccgt ttggctggcg cgaagtgtgg atcggttatg acccggcaaa aggtacgcag
     3121 aacggcgaca gcgccggatg cgtggtggtg gcaccgccag ccgtgccagg cggtaagttc
     3181 cgcattcttg agcgtcacca gtggcgcgga atggactttc gcgcccaggc tgacgccatc
     3241 aaaaaactga ctgaacagta caacgtgacc tatatcggca tcgactcaac cggcgttggt
     3301 cacggggttt acgagaacgt gaaagcgttt tttcctgccg tccgggagtt tgtctacaac
     3361 cccaacgtta aaaacgccct ggtactcaag gcctacgaca ttatcagcca ccgccgtctt
     3421 gagtttgacg ccgggcacac cgacattgcg cagtcattca tggcaatccg tcgcgccacc
     3481 accgccagcg gcaaccgccc gacctatgaa gccagccgca gcgaagaagc cagccatgcc
     3541 gatctggcct gggcaacaat gcacgcactg tttaacgaac cgctgcaggg cgagtccgcc
     3601 aataccagca atattgtgga gattttttga tgggaaagag taagaagaac cgcgctgcgg
     3661 cgacgaaaca gacccagcat aaaaaccaga cttcagccga agcattcagc ttcggcgatc
     3721 ccgttccggt tctggaccgc cgtgaactac tggactatgt ggaatgcgta cagacagatc
     3781 gctggtatga gccgccagtg agttttgacg gactggcgcg caccttccgc gccgccgtgc
     3841 atcacagttc accaattgcg gtgaaatgca acattctgat cagtacctac atccctcacc
     3901 cgctgctcag ccagcaggct ttttcacgtt ttgtgcagga ttatctggta tttggtaacg
     3961 cttacctgga gaaacgcaca aaccggttcg gagaggtcat cgctcttgag cctgctctgg
     4021 caaaatacac ccgacgcgga ttagacctgg atacctactg gtttgtgcaa tacggcatga
     4081 ccacgcagcc gtatcagttc acgaaaggaa gcatctttca tctgatggaa ccggacatca
     4141 accaagagat ctacggcctg ccaggttacc tttctgccat tccgtcagcc ctgctcaacg
     4201 agtccgccac gctgttccgc cgatagtatt acattaacgg cagtcatgca ggcttcatca
     4261 tgtacatgac cgatgccgcg caaaaccagg aggatgtgaa caacctccgc aatgcgatga
     4321 aaagcgccaa aggaccaggt aacttccgca atctgtttat gtactcgcct aacggcaaaa
     4381 aggacgggct tcagatcatc ccgttgtcag aagtcgcggc gaaggatgag tttctgaaca
     4441 tcaagaacgt gagccgggat gacatgatgg cggcgcatcg tgtgccaccg caaatgatgg
     4501 ggattatgcc taataatgtc ggggggtttg gggatgtgga gaaggcgagc ctggagtttg
     4561 tacgtaacga gttaataccg ttacaaaaaa gaatgatgga aataaataaa ttggtaagtg
     4621 agaacattgt taaatttgat agttataaac tagctactta acgtaatcac taaatgaggt
     4681 tctttaacac agctaaaaaa tttattaata gccagcagtt tttatgaatt tcttcagcgt
     4741 gcagatacac gctgaagatg caaaaaatca tatgagacga ccgtctggac ttctgttacc
     4801 taaatgtaat tcacagagta tccacctaat cacatcaggt cgaattaacc cagctacaaa
     4861 aaccgaattc caattatttt tacctttatg tttttcatcc aacagcaaca actcgcgagt
     4921 caacaactca tatacatcgc tataagtaga aagactctta ttcagtcctc taataacaag
     4981 ctcaggatat aagtaaaacg actcccattg ttcagacaaa taccccttaa tattattctt
     5041 gataacaaca ttatcaagat gatacttaac attgttatat tctggtgaca ttgggtcaat
     5101 tatgattttt acatcaattg taggtgttcg ccctaattta gaaaaattag cacatattaa
     5161 tctttgtttt aatatattat caaaatatgg atgcagcatc cgatggcctg cagaattagc
     5221 tatagtagta cttttgaaag tattacattt gcaagcagga accaaattct tagtgaataa
     5281 agaaaattct ggatagtttt ccttgggtaa aacatgatcc aaagtcccgc tatgcattga
     5341 accacacatt ggacatagag agttagaatt agctgctctt atatcatcaa ttatagataa
     5401 acactttgcg ggattgttat aataatatct caaagcttca gcaatattgt tactcaatac
     5461 gccacaagaa ttgacattaa gatacccttt attctttatg tacgatctat atgcattaag
     5521 tataaatgta tcctgtccat tcagaacaga atgatgtttt aatctcttat ttgcagatag
     5581 ttttttaaat gcagaataat ctttaaaaac aggtcttttt aatttattca tttcaattgc
     5641 ccatttcatt tctcaacgca gttaatacat taagtgataa gtcctcgcta tatttagatt
     5701 caacatcatc ccaagacata ttctttgcaa ttatattgtt ttttacttta tttaaaattt
     5761 gagttggttc actctcaccg aaaacaaaat atgagatatt tcctatattc gccccaaaag
     5821 tactcaatct cggtttttcg ctaacaatta ccccatcatc tcgtcttctg agtattgtca
     5881 cttgctcctt aaaaacctcc cgaacaagat aaaccgaatg cgttgctatg atagctgaag
     5941 agcctgtctt ttctaataaa ttatcaagca atgccataaa tttattaata aatgaaggat
     6001 ggagatgtgt ttctggctca tctattaata gaagagtccc attctcaata tacaaacaga
     6061 cttgtacaca aaacttaata aacgatattt ccccactact caaagggtaa catttatttt
     6121 caataagcct caaaggctcg ccagagagaa taatttcaga atataatgat agtctagatt
     6181 cttcgcctcg agaattaagc tcatttaatt taacaaatgg tgaattttca ccgccacatt
     6241 tgattactaa atcgttgtta ctatttagaa cactcagtgc atcaacaaat atattccacc
     6301 tttgattata gccgatagcg tcgttactcc tcgctagttg cactattaaa tcattcaagt
     6361 ttaatttgga ttttgattca cgatttaaag tcaatctttt gtaccatatc aatgatttta
     6421 ttcttttatc ggttggaaat gaacacttac tttcatttgt tggagaaaaa gcaatgagcc
     6481 tactaatttg aattcgttct tcaatgctca caccgttaga atcaactaca gccaccaaat
     6541 tatcctttcc tttaatcgca gccaatgcga tttctctaag cgtttggctt tttcctacac
     6601 cattctcgcc tataattatg gaaattcttt ttggtaaatc atgctcgtga tcaaaattaa
     6661 aaacataaga tacatcttct tcattaactt tattcgaata gctaatcttg atgtgctgtg
     6721 ataaattacc caactcctca tattgaatac cggaaagaac tgttcctgca tttttgaaag
     6781 taaaatacga ctcagactct ctaatgaaag atttattaaa gatatcagaa gagattgctt
     6841 ttttcctcaa attctctgca ctagcgtcac ttttcttaga aacaacatca ttaatgaaat
     6901 ttaatatata tttggattca ttcaccccaa aaaatcttac tagtcttcga taattattca
     6961 tatcctgaag catgacaaaa taatcattaa acccctcatc ctttaaatta gattcttcct
     7021 ttttcttcac tcgccttatc agttctgctt ttccatttcg ttcctcctct gaattcaaaa
     7081 aaccaatata accattcagt gaaattatat ccttacactc ttttttatta aaaatagata
     7141 tttcgactct actcaaatat ccaaaatcat tccaatcgct tcttgatggg actattttta
     7201 tataattacc agaatcagat atctgttgat tatttgtaat taaaaaatat aaactataat
     7261 ttttattttt cttgactaac attattaccc tctcacttca ttagtgatga tttattttgg
     7321 aattatttta cacacttata cctagcgcgc aatcgtatcc ccgccacgcc tgcccgcttt
     7381 atgtagaggt tttcatgcac ctgcatgatc tacgcaaaag cccgccagtt ctggcgggcc
     7441 ttagcaaaaa cgatcctcaa acgatcatgc gatctcatgc ggcatagaca tgcactacag
     7501 agctaacgcc tcgcaagggc tcgttgttca accttgctga cgccagaagc tagttcagac
     7561 gccagcaacg tttcttaatg cagccagctg tcgtcttccc acaccttctg cataattttc
     7621 atcacttgtt ttctttcttc gtccagttgc aatccagtaa gttccacacc gttagagcta
     7681 cctttgcgga tacgaattac cgttttggga tacagagggc gcagattgcg gtaaagctcg
     7741 gattcaagag cgtccagggt agactggcta atcttctgct ctttatcgat cattatttca
     7801 atgcgcataa aagtcacctc agctgatgac atccattgag cggttgtatt cgtgggttct
     7861 gatttttgcc atgagttcat cagtcaattc agaaacccac tgcagagcca gccccttctc
     7921 ttcatcacta cactcactag ccgctacaag cttaagaaaa aaatcaatgc gctggagctt
     7981 caaagactcc aaaaaatagt cctgcatctt tcctcctatg acaccacacg caatactgta
     8041 tgcataacca ctgtttatat ttacagtata taataatctt actgatgtaa aacgtttttt
     8101 tacgttcatc agcctgatat gcctggtatt attaagagca cgaattgtta acccgcgtaa
     8161 ttaatacagg ttccgccact gatcatcttc ctgcaaacgc tggttccgat agaagatacg
     8221 caggcctgct cctgacggaa tactgcctcc gcgaaggagt aaatcgacct ctttctcgct
     8281 gccatcaaat cctctggact tcagctcata cacgagctgc agtcgctgat ggtctgtaat
     8341 tcgctgtttg tagtctttac gccgtttcgg tttcaccagg cgtaaccttg ctgccagttc
     8401 ccggcgctct tttttgctca tactgtgcag gtaatcgtgc aactccttgt catccatgct
     8461 ggtaatgtcc gttctggggt tcccatcagc tgatttatct ttctcctgtt ggttcaaatt
     8521 ttcagcaagg agacagttat tgccacgagt ccaaggggcg caagcgccct ggtcggctgc
     8581 cgcctcctga acgtcaacgg ctttacgaac cattttccac ttcactgcat gagtgcagat
     8641 cttgccttct gcaatgggtg accagatgcc ataaatacga ataccgtgat cgccataggc
     8701 ggtcggctct tcattgattt cataagcggt tctgatcaag tgatatttgc gaggaaccag
     8761 cacgccgccc tgcctcataa tgtaggtggc aaaacagcca gcatcagcag cagccaggat
     8821 ggcatcaagg cgcgggttat ccagtaccgg cgcacctgct tttttgtcac cctgctgcct
     8881 tgccgcctga ccagccaaca accgcagttc acggtaagcc tgacgccctg gaataccaaa
     8941 gaagcggaat tgctgaacac gatgcagaga cgcccaggga ttaacgtatt cagcgttatc
     9001 acgcagggat ttacccgttt ccttgctgat ctcgccagcc agaccacgcc cgtcaatgtt
     9061 cttactgatg tatttcgcga tgtagcttgt cggcgttcct ttgcgcgggt ttatcagctc
     9121 agatttaaag cgtggaccag tgttattacc cagttcctcg cggtcttcac ggatggcaaa
     9181 cttacgcaac aatgcagtaa tggcgcggcg gtcttttttg cgcataaaac acaacaggtg
     9241 ccagtgaact gtaccgtcat gatgcggctc agccacccgc acgccatacc agcgcaaccc
     9301 ggctttgtgc atcgccttac gaaatgcagc aaacatgccg accagataat cgctgctttg
     9361 tcttaccgtc gcatttgtcc aggttgggtt tggtctgccg ttattgagcg tggaatggaa
     9421 acgtgacgga caggtaatgg tgtagaaaac ggcgcagtca ccgcgcattt ccgcgataag
     9481 ctccagacct ttaacacagg ccatcatctc attgcggcgg tgcgccgggt tgctgctgct
     9541 ggcgtttacc acgtcttcca tatccagcgt gtcgccctct tcgttcacca gttcatgaga
     9601 acggaaaaac tccaatgact tgcggcgctg ctcacgttta tgcatcacgg cttcatagct
     9661 gacataggga gatgcttttt tgctgaccag gcagacagca cgcaactgct cttcccgcca
     9721 ttcgcaacgc attttccata atttccggta ccaccagtcg gcgcacaaca tacgcgccag
     9781 cgaacccgga atgagttcat agggcacggg tttacggcgg tttcttttcc gacggagttg
     9841 ctcaaacgca ggcgggatga catccagacg cagggtttcc gctgccacct tttcccatgt
     9901 cttgcggatt tcttctggct taacgtcatc ggtggcatac aaatcaccac aagctgcatc
     9961 aaggcacatg ctcatatgcg cagcgacaag ggtagacagg cgtttcacct gatcctgact
    10021 catttcaggc agaatcagca ggccgtccag ccctacatgg cttgccataa aacgaaaaga
    10081 tgcagatagc tggctgtcgc gtacatgctc cagtcgttcc agacatggct taatcgtctc
    10141 acgtaaatag cgggaataag cctttggcct gcccaggctg ctgaagtatt caatacgttg
    10201 catcagcggc ttgctgatat gggaaggctg ggcgttgacg tccgccagaa tgaccatatc
    10261 cggattaaaa cgctgctgct catgcgccag ctttgcccgg ctaatgagct tatcctgctc
    10321 catttcgtgc tggacaggat cacgtgattc ataaaaaaaa taacgctccc agacctgatc
    10381 actcagtgcc tcgcggcgca actgttcctg ctcgttatcg gcagcgtaca gagtgatcag
    10441 gtttgaaagc gcagaaaccg gcgcaacttc cgccgggtcc agataagggt taatggcctt
    10501 tttcgggctg ttccatgaga acgctgcggc agcctcgtta aagccgcagc agttgttcat
    10561 atcggcatgg ctcatgcacg tactccgtac acggcagaac tatccacgcc acgcgaataa
    10621 tcaaatccca cccagcagcg cggcccggaa acagcaatga tttctgttgc tgatttaccc
    10681 tcgccagctg ccacaccgat gctgcgtttt accttgatat agtggtgagt aaaattgcga
    10741 tacagcgaac ggatcaggga tgtgtcactg ttagaaacaa tgaccggatg tccttctgat
    10801 gaccgatgtt caagaacgga tgctaggtga tactggtcat cttcagtgaa accatcagtg
    10861 tgatagccgg aaaacgtacc gtcatacggt ggatcgcaat acaccacatc ccccgccttc
    10921 aacatcgcca gcgtttcatc aaagctggcg cagataaacg ttgctcgctg ggctttttct
    10981 gcaaatgcgc gaatttcttt ttcagggaaa tacggatttt tataattgcc gtagggaatg
    11041 ttgaaatgcc cgctcttgtt atagcgacat aaaccacggt aaccgtgacg attgagatac
    11101 aggaaatata ccgctttcat gaaatcagta atttcagttg agtaattaaa ctcctgcctt
    11161 atgttgtaat aagccacctc cctgtttgcg atctcaaata aaactctgg
//
LOCUS       DADLTD010000142        11068 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLTD010000142 DADLTD010000000
VERSION     DADLTD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.142"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="JN701_002936"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="JN701_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7365312.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            923..1000
                     /locus_tag="JN701_002937"
                     /pseudo
     CDS             923..1000
                     /locus_tag="JN701_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(1207..2058)
                     /locus_tag="JN701_002938"
     CDS             complement(1207..2058)
                     /locus_tag="JN701_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAY7365313.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     gene            complement(2045..2884)
                     /locus_tag="JN701_002939"
     CDS             complement(2045..2884)
                     /locus_tag="JN701_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RepA"
                     /protein_id="HAY7365314.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
                     MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
                     ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
                     GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
                     EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
                     GEA"
     gene            complement(2914..3120)
                     /locus_tag="JN701_002940"
     CDS             complement(2914..3120)
                     /locus_tag="JN701_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365315.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDE
                     YEALRECLEELRAAQGGGSDPASA"
     gene            complement(3122..3334)
                     /locus_tag="JN701_002941"
     CDS             complement(3122..3334)
                     /locus_tag="JN701_002941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365316.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMA
                     SLNLDGCTLSLFREDKPFGPGKFLGD"
     gene            complement(3395..5524)
                     /locus_tag="JN701_002942"
     CDS             complement(3395..5524)
                     /locus_tag="JN701_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein A"
                     /protein_id="HAY7365317.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAES
                     GHMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGA
                     ERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEAL
                     KPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGR
                     GIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEH
                     GDTGRRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERP
                     ERVAGVALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRT
                     LQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIR
                     PAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQ
                     IARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGP
                     ALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSV
                     QLARAELARAPAPRQRGMDRGGPDFSM"
     gene            5723..6007
                     /locus_tag="JN701_002943"
     CDS             5723..6007
                     /locus_tag="JN701_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="HAY7365318.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRR
                     KVLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            6469..6906
                     /locus_tag="JN701_002944"
                     /pseudo
     CDS             6469..6906
                     /locus_tag="JN701_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(6837..8036)
                     /gene="tet(A)"
                     /locus_tag="JN701_002945"
     CDS             complement(6837..8036)
                     /gene="tet(A)"
                     /locus_tag="JN701_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY7365319.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            8142..8792
                     /gene="tetR(A)"
                     /locus_tag="JN701_002946"
     CDS             8142..8792
                     /gene="tetR(A)"
                     /locus_tag="JN701_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY7365320.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKQALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(8824..9066)
                     /locus_tag="JN701_002947"
     CDS             complement(8824..9066)
                     /locus_tag="JN701_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY7365321.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(9346..9549)
                     /locus_tag="JN701_002948"
                     /pseudo
     CDS             complement(9346..9549)
                     /locus_tag="JN701_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein (MobL-like)"
     gene            complement(9453..10289)
                     /locus_tag="JN701_002949"
     CDS             complement(9453..10289)
                     /locus_tag="JN701_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7365322.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(10289..>11068)
                     /locus_tag="JN701_002950"
                     /note="aph(3'')-Ib"
     CDS             complement(10289..>11068)
                     /locus_tag="JN701_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7365323.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         2107 a         3437 c         3397 g         2127 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 ggggcttcta taataataat aatcgagcat gagtatcata cggatgctcg ggtcgaaagg
      961 gaatccccag gcgagtaacc tgtttgcggt gatccattag ctgcaggagc agaagagcat
     1021 acatctggaa gcaaagccag gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc
     1081 aatttttcaa aatattgtta agccttttct gagcatggta tttttcatgg tattaccaat
     1141 tagcaggaaa ataagccatt gaatataaaa gataaaaatg tcttgtttac aatagagtgg
     1201 ggggggtcag cctgccgcct tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt
     1261 taccgtccag cccagcgcga ccagctccgg caacgcctcg cgcacccgct ggcggcgctt
     1321 gcgcatggtc gaaccactgg cctctgacgg ccagacatag ccgcacaagg tatctatgga
     1381 agccttgccg gttttgccgg ggtcgatcca gccacacagc cgctggtgca gcaggcgggc
     1441 ggtttcgctg tccagcgccc gcacctcgtc catgctgatg cgcacatgct ggccgccacc
     1501 catgacggcc tgcgcgatca aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc
     1561 gctggcgtac tccgacagca gccgaaaccc ctgccgcttg cggccattct gggcgatgat
     1621 ggataccttc caaaggcgct cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc
     1681 gacctctgcc ccgatttcct ttgccagcgc ccgatagcta cctttgacca ccatggcatc
     1741 agcggtgacg gcctcccact tgggttccag gaacagccgg agctgccgtc cgccttcggt
     1801 cttgggttcc gggccaagca ctaggccatt aggcccagcc atggccacca gcccttgcag
     1861 gatgcgcaga tcatcagcgc ccagcggctc cgggccgctg aactcgatcc gcttgccgtc
     1921 gccgtagtca tacgtcacgt ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg
     1981 gaacaggccg ggggccagac agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt
     2041 gttcttaggc ttcaccacgg ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg
     2101 gcttgagcac cccgccgtca tgccgcctga accaccgatc agcgaacggt gcgccatagt
     2161 tggccttgct cacaccgaag cggacgaaga accggcgctg gtcgtcgtcc acaccccatt
     2221 cctcggcctc ggcgctggtc atgctcgaca ggtaggactg ccagcggatg ttatcgacca
     2281 gtaccgagct gccccggctg gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct
     2341 tgctggcatg gtgcaggaac acgatagagc acccggtatc ggcggcgatg gcctccatgc
     2401 gaccgatgac ctgggccatg gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca
     2461 gcgtgtccag caccatcagg cggcggccct cggcggcgcg cttgaggccg tcgaaccact
     2521 ccggggccat gatgttgggc aggctgccga tcagcggctg gatcagcagg ccgtcagcca
     2581 cggcttgccg ttcctcggcg ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg
     2641 cggtgggcgg gtcttcggcg ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct
     2701 ccagcagatc cggcccgcct gcaatctgtg cggccagttg cagggccagc atggatttac
     2761 cggcaccacc gggcgacacc agcgccccga ccgtaccggc caccatgttg ggcaaaacgt
     2821 agtccagcgg tggcggcgct gctgcgaacg cctccagaat attgataggc ttatgggtag
     2881 ccattgattg cctcctttgc aggcagttgg tggttaggcg ctggcggggt cactaccccc
     2941 gccctgcgcc gctctgagtt cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt
     3001 cagccagaac ttgcgctgac gcatcccttt ggccttcatg cgctcggcat atcgcgcttg
     3061 gcgtacagcg tcagggctgg ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat
     3121 atcagtcacc gagaaacttg ccggggccga aaggcttgtc ttcgcggaac aaggacaagg
     3181 tgcagccgtc aaggttaagg ctggccatat cagcgactga aaagcggcca gcctcggcct
     3241 tgtttgacgt ataaccaaag ccaccgggca accaatagcc cttgtcactt ttgatcaggt
     3301 agaccgaccc tgaagcgctt ttttcgtatt ccataaaacc cccttctgtg cgtgagtact
     3361 catagtataa caggcgtgag taccaacgca agcactacat gctgaaatct ggcccgcccc
     3421 tgtccatgcc tcgctggcgg ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga
     3481 cgctgggcag acccatgacc ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg
     3541 gcttgcgctc tgccagcgct gggctggcct cggccatggc cttgccgatt tcctcggcac
     3601 tgcggccccg gctggccagc ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac
     3661 cgaagcgctt gaccagcccg gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt
     3721 ggcggctaag ctgccgctcg ggcagttcga ggctggccag cctgcgggcc ttctcctgct
     3781 gccgctgggc ctgctcgatc tgctggccag cctgctgcac cagcgccggg ccagcggtgg
     3841 cggtcttgcc cttggattca cgcagcagca cccacggctg ataaccggcg cgggtggtgt
     3901 gcttgtcctt gcggttggtg aagcccgcca agcggccata gtggcggctg tcggcgctgg
     3961 ccgggtcggc gtcgtactcg ctggccagcg tccgggcaat ctgcccccga agttcaccgc
     4021 ctgcggcgtc ggccaccttg acccatgcct gatagttctt cgggctggtt tccactacca
     4081 gggcaggctc ccggccctcg gctttcatgt catccaggtc aaactcgctg aggtcgtcca
     4141 ccagcaccag accatgccgc tcctgctcgg cgggcctgat atacacgtca ttgccctggg
     4201 cattcatccg cttgagccat ggcgtgttct ggagcacttc ggcggctgac cattcccggt
     4261 tcatcatctg gccggtggtg gcgtccctga cgccgatatc gaagcgctca cagcccatgg
     4321 ccttgagctg tcggcctatg gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg
     4381 cagccagatc gagccgtcct cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc
     4441 gatcagcagc accaccgtag gcatcatgga agccagcatc acggttagcc atagcttcca
     4501 gtgccacccc cgcgacgcgc tccgggcgct ctgcgcggcg ctgctcacct cggcggctac
     4561 ctcccgcaac tctttggcca gctccaccca tgccgcccct gtctggcgct gggctttcag
     4621 ccactccgcc gcctgcgcct cgctggcctg ctgggtctgg ctcatgacct gccgggcttc
     4681 gtcggccagt gtcgccatgc tctggaccag cggttcgatc tgctccgcta actcgttgat
     4741 gcctctggat ttcttcactc tgtcgattgc gttcatggtc tattgcctcc cggtattcct
     4801 gtaagtcgat gatctgggcg ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg
     4861 tgcggatgcc ccggccttcc atctccacca cgttcggccc caggtgaaca ccgggcaggc
     4921 gctcgatgcc ctgcgcctca agtgttctgt ggtcaatgcg ggcgtcgtgg ccagcccgct
     4981 ctaatgcccg gttggcatgg tcggcccatg cctcgcgggt ctgctcaagc catgccttgg
     5041 gcttgagcgc ttcggtcttc tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct
     5101 tgaaccactg agcggcgggc cgctcgatgc cgtcattgat ccgctcggag atcatcaggt
     5161 ggcagtgcgg gttctcgccg ccaccggcat ggatggccag cgtatacggc aggcgctcgg
     5221 caccagtcag gtgctgggcg aactcggacg ccagcgcctt ctgctggtcg agggtcagct
     5281 cgaccggcag ggcaaattcg acctccttga acagccgccc attggcgcgt tcatacaggt
     5341 cggcagcatc ccagtagtcg gcgggccgct cgacgaactc cggcatgtgc ccggattcgg
     5401 cgtgcaagac ttcatccatg tcgcgggcat acttgccttc gcgctggatg tagtcggcct
     5461 tggccctggc cgattggccg cccgacctgc tgccggtttt cgccgtaagg tgataaatcg
     5521 ccatgctgcc tcgctgttgc ttttgctttt cggctccatg caatggccct cggagagcgc
     5581 accgcccgaa gggtggccgt taggccagtt tctcgaagag aaaccggtaa gtgcgccctc
     5641 ccctacaaag tagggtcggg attgccgccg ctgtgcctcc atgatagcct acgagacagc
     5701 acattaacaa tggggtgtca agatggttaa ggggagcaac aaggcggcgg atcggctggc
     5761 caagctcgaa gaacaacgag cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga
     5821 acagcagcaa gagcgcaaga acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt
     5881 ggccaaggtg aacagcagcg agtggccgga ggatcggctc atggcggcaa tggatgcgta
     5941 ccttgaacgc gaccacgacc gcgccttgtt cggtctgccg ccacgccaga aggatgagcc
     6001 gggctgaatg atcgaccgag acaggccctg cggggctgca cacgcgcccc cacccttcgg
     6061 gtagggggaa aggccgctaa agcggctaaa agcgctccag cgtatttctg cggggtttgg
     6121 tgtggggttt agcgggcttt gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg
     6181 tagcctagcg cagcgaatag accagctatc cggcctctgg ccgggcatat tgggcaaggg
     6241 cagcagcgcc ccacaagggc gctgataacc gcgcctagtg gattattctt agataatcat
     6301 ggatggattt ttccaacacc ccgccagccc ccgcccctgc tgggtttgca ggtttggggg
     6361 cgtgacagtt attgcagggg ttcgtgacag ttattgcagg ggggcgtgac agttattgca
     6421 ggggttcgtg acagttagta cgggagtgac gggcactggc tggcaatgtc tagcaacggc
     6481 aggcatttcg gcgagatccg attgtcggcc ctgaagaaag ctccagagat gttcgggtcc
     6541 gtttacgagc acgaggagaa aaagcccatg gaggcgttcg ctgaacggtt gcgagatgcc
     6601 gtggcattcg gcgcctacat cgacggcgag atcattgggc tgtcggtctt caaacaggag
     6661 gacggcccca aggacgctca caaggcgcat ctgtccggcg ttttcgtgga gcccgaacag
     6721 cgaggccgag gggtcgccgg tatgctgctg cgggcggtga tagactatgg ccgcgagcat
     6781 gtggagcaga tcatgctgac ggttgcggaa gggaacgaag ccgctataaa cctctatcag
     6841 cgatcggctc gttgccctgc gccgctccaa agcccgcgac gcagcgccgg caggcagagc
     6901 aagtagaggg cagcgcctgc aatccatgcc cacccgttcc acgttgttat agaagccgca
     6961 tagatcgccg tgaagaggag gggtccgacg atcgaggtca ggctggtgag cgccgccagt
     7021 gagccttgca gctgcccctg acgttcctca tccacctgcc tggacaacat tgcttgcagc
     7081 gccggcattc cgatgccacc cgaagcaagc aggaccatga tcgggaacgc catccatccc
     7141 cgtgtcgcga aggcaagcag gatgtagcct gtgccgtcgg caatcattcc gagcatgagt
     7201 gcccgccttt cgccgagccg ggcggctaca gggccggtga tcattgcctg ggcgagtgaa
     7261 tgcagaatgc caaatgcggc aagcgaaatg ccgatcgtgg tcgcgtccca gtgaaagcga
     7321 tcctcgccga aaatgaccca aagcgcggcc ggcacctgtc cgacaagttg catgatgaag
     7381 aagaccgcca tcagggcggc gacgacggtc atgccccggg cccaccggaa cgaagcgagc
     7441 gggttgagag cctcccggcg taacggccgg cgttcgcctt tgtgcgactc cggcaaaagg
     7501 aaacagcccg tcaggaaatt gaggccgttc aaggctgccg cggcgaagaa cggagcgtgg
     7561 ggggagaaac cgcccatcag cccaccgagc acaggtcccg cgaccatccc gaacccgaaa
     7621 caggcgctca tgaagccgaa gtgccgcgcg cgctcatcgc catcagtgat atcggcaata
     7681 taagcgccgg ctaccgcccc agtcgccccg gtgatgccgg ccacgatccg cccgatatag
     7741 agaacccaaa ggaaaggcgc cgtcgccatg atggcgtagt cgacagcagc gccggccagc
     7801 gagacgagca agaccggccg ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg
     7861 caggcaaatt gcatcaacgc atacagcgcc agcagaatgc catagtgggc ggtgacgtcg
     7921 ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg gcataatcag gccgatgccg
     7981 acagcgtcga gcgcgacagt gctcagaatt acgatcaggg gtctgttggg tttcacgtct
     8041 ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg gattctttat cactgataag
     8101 ttggtggaca tattatgttt atcagtgata aagtgtcaag catgacaaag ttgcagccga
     8161 atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt cggcgtagac ggtctgacga
     8221 cacgcaaact ggcggaacgg ttgggggttc agcagccggc gctttactgg cacttcagga
     8281 acaagcaggc gctgctcgac gcactggccg aagccatgct ggcggagaat catacgcatt
     8341 cggtgccgag agccgacgac gactggcgct catttctgat cgggaatgcc cgcagcttca
     8401 ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg
     8461 caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg
     8521 ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt cactgttggg gccgtgcttg
     8581 aggagcaggc cggcgacagc gatgccggcg agcgcggcgg caccgttgaa caggctccgc
     8641 tctcgccgct gttgcgggcc gcgatagacg ccttcgacga agccggtccg gacgcagcgt
     8701 tcgagcaggg actcgcggtg attgtcgatg gattggcgaa aaggaggctc gttgtcagga
     8761 acgttgaagg accgagaaag ggtgacgatt gatcaggacc gctgccggag cgcaacccac
     8821 tcactacagc agagccatgt agacaacatc ccctccccct ttccaccgcg tcagacgccc
     8881 gtagcagccc gctacgggct ttttcatgcc ctgccctagc gtccaagcct cacggccgcg
     8941 ctcggcctct ctggcggcct tctggcgctc ctgctgcggc gtccgctcgt gggccgtggc
     9001 gcgggtccgc gcgccggcct cgtgcgcctg gcgctcgcgg gcgaggtcca gggcggccgt
     9061 cttcacgttc tgccttgcgc agatgagata gatctagcgt ggactcaagg ctctcgcgaa
     9121 tggctcgcgt tggaaacttt cattgacact tgaggggcac cgcagggaaa ttctcgtcct
     9181 tgcgagaacc ggctatgtcg tgctgcgcat cgagcctgcg cccttggctt gtctcgcccc
     9241 tctccgcgtc gctacggggc ttccagcgcc tttccgacgc tcaccgggct ggttgccctc
     9301 gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaagggg tcgtttgcgg
     9361 gaaggggcgg aatcctacgc taaggctttg gccagcgata ttctccggtg agattgatgt
     9421 gttcccaggg gataggagaa gtcgcttgat atctagtatg acgtctgtcg cacctgcttg
     9481 atcgcggccg cgatagctag atcgcgttgc tcctcttctc catccgcgtt ccaagctgcg
     9541 gaaaggcacc cataagcgta cgcctggtcg agcaggcgac gcggatcgac gtccagcgca
     9601 cgagagaatg cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag gtcgtctctg
     9661 tcagccggat cgtagaacat attggcggcg ccaaagccca cttcaccgac cagaccgacg
     9721 ggatctatca ccagccagcc gcgactggag aacatgatgt tttcatgatg cagatcgcca
     9781 tgtagcccac gcagttccga ggcattgctc atcatttgat cggctataat cgccgcgtgg
     9841 acgtagtcag tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa caaagctgca
     9901 aagcgatccc ggatcgggag aagggcagaa ggcaggggtt cctcagatgc ggcatacagc
     9961 ttcgccatta gttccgctgc aatttcggtc gcctggtagt cgccgtgctc ggcaacgatg
    10021 tgagagagca ttcgctcccc ggcatattcg agcaacatca gattgttctc acgaccgagc
    10081 aaccggactg ctcccctccc attgcgccat accagatagt cggccccgcg cagttcatca
    10141 gcaatgtctt ctataggttt caatcccttg acgattgcag gagtcccgtc tggcaatgaa
    10201 actttccaaa cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga aacgtgccaa
    10261 tgagcaggaa aaacaggcgg catgaacatc aaccccaagt cagagggtcc aatcgcagat
    10321 agaaggcaag gcgttcgcgg tcgggggctt cgatccccaa tacattgaat aggacagcga
    10381 aggcgcgctc tgcttcatct ggcgctgccc agttctcttc ggcgttagca atcatgagtg
    10441 ccaaatcggc atagcgatct gctgttccga gccgcccaag gtcgatcaga cccgtgcatt
    10501 gaagagtttt agggtccacc atgaagttcg gcatgcaggg atcaccatgg caaacaacca
    10561 tatcggtgcg ctcttggtcg agccgcaccg gtagctctcg ttcgacacga gccaaaagat
    10621 cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc gggattgacg gcattgcggg
    10681 acaccacatc aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac ggacattgat
    10741 caaccgatag gctgtgaaca gcgccaagtt gctgccccat tgacggccac gctttgagca
    10801 aatccgctcc agacagatca gccgccggta ctcccggaat tgccgttatc accaagcatg
    10861 caccctcctg ttcctcctgc cagttgatga cctcggggca agccacacct cgacctttga
    10921 gccaaatgag gcggtcacgc tctccagcga gctcaccgcg gcgggaagca ggtgcgattt
    10981 tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc accagattct ccgcctctga
    11041 caggcaacca gtcagaatgc gattcacc
//
LOCUS       DADLTD010000143        10975 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLTD010000143 DADLTD010000000
VERSION     DADLTD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.143"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1063)
                     /locus_tag="JN701_002951"
     CDS             complement(<1..1063)
                     /locus_tag="JN701_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7365324.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGD"
     gene            complement(1642..2319)
                     /gene="bioD"
                     /locus_tag="JN701_002952"
     CDS             complement(1642..2319)
                     /gene="bioD"
                     /locus_tag="JN701_002952"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7365325.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQPAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(2312..3067)
                     /gene="bioC"
                     /locus_tag="JN701_002953"
     CDS             complement(2312..3067)
                     /gene="bioC"
                     /locus_tag="JN701_002953"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7365326.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTSHLFLGVIARE"
     gene            complement(3054..4208)
                     /gene="bioF"
                     /locus_tag="JN701_002954"
     CDS             complement(3054..4208)
                     /gene="bioF"
                     /locus_tag="JN701_002954"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY7365327.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(4205..5245)
                     /gene="bioB"
                     /locus_tag="JN701_002955"
     CDS             complement(4205..5245)
                     /gene="bioB"
                     /locus_tag="JN701_002955"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY7365328.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            5332..6621
                     /gene="bioA"
                     /locus_tag="JN701_002956"
     CDS             5332..6621
                     /gene="bioA"
                     /locus_tag="JN701_002956"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY7365329.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDGTFFCQ"
     gene            6680..7156
                     /locus_tag="JN701_002957"
     CDS             6680..7156
                     /locus_tag="JN701_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY7365330.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            7308..8591
                     /locus_tag="JN701_002958"
     CDS             7308..8591
                     /locus_tag="JN701_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY7365331.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(8732..10969)
                     /locus_tag="JN701_002959"
     CDS             complement(8732..10969)
                     /locus_tag="JN701_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY7365332.1"
                     /translation="MFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGNMD
                     KLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVF
                     GLSAAQRYGGIFVPPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGE
                     LVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVG
                     PGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYY
                     DGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLL
                     DKVENSRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDL
                     AKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQM
                     SAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQ
                     PLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEF
                     TLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMV
                     YAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEV
                     GDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFN
                     KNRQM"
BASE COUNT         2501 a         3010 c         2834 g         2630 t
ORIGIN      
        1 ggtcaccgcg atacatgccg ccaatttgtg gaatggtgac gtgagattca tcgacgacca
       61 gcagcccatc ggcaggcagg taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac
      121 cggagaggaa gcgcgagtag ttttcaatcc ccgaacagta gcccagctcg ttcatcatct
      181 ccagatcaaa ctgggtacgc tgggtcagcc gctgctcttc cagcagtttg ttgttttcca
      241 acagcacttt gcgtctggcg gccagttctt ctttgatctc ctccatcgcc tgtacgatgc
      301 gctcgcgcgg tgtgacgtag tgcgttttcg ggtagatggt aaaacgtgga atagtggaaa
      361 caatctgccc ggtcagcggg tcaaataacg acaatcgttc cacttcctcg tcaaacagtt
      421 ccacgcgaag tgcaatgtca tccgattctg ccgggaagat atcaatcacc tcgccacgaa
      481 cgcggaaagt accacgctgg aatgcttgat cattacgagc gtattgcagc tccgccagtc
      541 ggcgcagaat cgcgcgctga tcgataatca taccgaccgt gagatggagc atcatcttga
      601 gatataaatc aggatcgccc agaccataaa tcgcggaaac agaggccact acaaccacat
      661 cacgccgctc cagcatcgct ttggtggcgg ataaacgcat ctgctcaata tgttcgttaa
      721 ccgaggcatc tttctcaatg aaagtgtcgg aactcggtac ataggcttcc ggctgatagt
      781 agtcgtagta ggagacgaaa tactccaccg cgttttccgg gaagaactct ttcatttcgc
      841 catacagctg ggccgccagc gttttgttgg gcgcaagtac catggttggg cgctgaaggt
      901 cagcaatgac attggcaatg gtgaaggttt tccctgagcc agtcacgcca agtaacgtct
      961 ggtgcgccag gccatcttcc agcccctctt cgagacgtcg aatcgcctct ggctgatcgc
     1021 cagaaggttt aaaagcggaa ttcagtttga acggtttact catgagtcgc tacctgaagg
     1081 agttgggcgg gcaggtatgt aattttactc gtcgtactta attatgccaa caaattatac
     1141 tggataaaaa aacagttcat caccataata tttctgatac agcgtaaact ccgctgtcaa
     1201 tcatgagcaa aatttactct gtggcgagat aaaactccgg ccttaccggg ttatccccaa
     1261 agcaacggct tttttaacat ttgtcaagat gagtgatgac agttttatgg caagagatgc
     1321 ctgttcagtg actgccattg catttatcta accagttaaa aaataaaaga tatttctttg
     1381 agccgtcttt aacgccaatt cgcgtgcaag ccgcgtattc tctcgcttgc ctcgtgtttt
     1441 ctaactctaa tacacatggt tatccacagg aatagtggat aactgtctcc agcccctatc
     1501 ccccgccgcc tgggcaattc ccacattcgc cgcatgaggc ggaagtaaaa tttttttcgt
     1561 gattatttta gaatttaatt ggttaaattg cagtcaatcg aagacgcgat ctcgctcgca
     1621 atttaaccaa atacagaatg gctacaacaa ggcaaggttt atgtactttc cggttgccgc
     1681 attttctgga ttttctgcaa gccaggggat ctctcccagc agcggcgcgg gaatcatgcg
     1741 ggtgagcgtg gtcatatatt cagcgtgacg ttttcccgga ggcgtaacat cgttcgccac
     1801 ccaacccgcc agagtcagtc cggcgggttg tattgcctgc gcagtcaaca tcgcgtgatt
     1861 aatacagccg agtttcacac caactaccag tatcaccggc agttgttcct gtgttaccca
     1921 atctgcaaaa gtgaaagtgt cagaaagcgg cgtaaaccag ccgccagcac cttccactaa
     1981 cacccagtca gcctgttgtt caagcgcgcg taatccggcg ctcattacca atgattctat
     2041 cggcctgccc tcttgcgcgc tgatgatgtg cggcgaagtg ggttctgcga aggtgtaagg
     2101 atttactgtt gcgtaatcca gctgcaggct gctgttgtgc tgtaacgcca gcgcgtcact
     2161 attacgtaag ccttccgggg tcttttcgct gccagaggcg accggtttat aacctgccgt
     2221 ccgataacct actgccttcg cggcttgtaa aagtgcacaa ctggcgacgg ttttccccac
     2281 ttcggtatcc gttccggtga caaaataacg tttactcacg agcaatcact cccaaaaaaa
     2341 gatgagacgt cagaggatat cgcccctgtt gttgcggcca ggccagttgc aatcgctgca
     2401 attgcgaacg cgttaatatt cgcgggtcgc gcccttcatg aagatgcgta gcaccgatgc
     2461 ctttcagcga acgcatggca ctgagcgcat catcaaacca tagtgtgatg ggctgaatat
     2521 gatgttgata atgcacgccg ttcagcgact gttcgatttc atctggcggt aaaaagcgat
     2581 tagcatgcgg acgctcgtcc accgcctgcc acgcctgatg cagttcgggt aacgatccct
     2641 gcaccagcgt ggtaaacgcc accacgcctc cggggcgcac cacccgatac agctcgcgga
     2701 gtgccgtgga taaattaccg caccactgca ccgcgagatt gctccatgca agatcgaacg
     2761 tcgcagtcgc taacggcagg gattcgatat ctcccgccag ataatggtct gcagcatctt
     2821 tctggcgggc ttgcacaagc attggcggtg agagatctaa cgccgtcacc tgtgcgtgac
     2881 gctctcgcca gtggcggctc atccagcctg gcccacaccc cgcgtccagt acgtgggtgt
     2941 atttacgctg tgggagcatt gccagtaaga cgtcagcact ccggcgctgt aactctgcgt
     3001 gttgctcata gtgcgcggct gcccgaccaa atgccgctgc aatggcttgt ttattaaccg
     3061 ttgccatgca gcacctccag cagacggtcg atatcctgca tttcatgcgc tgcggttagc
     3121 gttaagcgca gtcgcgcagt accagcgggt acggttggcg ggcgaatcgc cgtgacccag
     3181 cagccttgct ggcgcagttt ttctgccagt tgtaacgcac ggctgttatc accgacgatc
     3241 aatggctgga tggcgctgca tgaatcagca agcgtaaacg gcaaatcctg tactccggca
     3301 cgaaaacgcg caatgagtga caccagtttt tcgcgccgtg catccccctc atcacggcga
     3361 atgaccgcca gcgacgcacg taatgcctgc gcctgagcgg gcggcatact ggtgttgtag
     3421 ataaggtggc gggcgaattg cagcagataa tccgccaccg tactggagca aagcaccgct
     3481 gccccgctga cgccaaatcc tttgccaaaa gtcaccacca gcaattctgg ttttaccttt
     3541 tgcagccagc agctgccgcg cccctgctcc ccgataacgc ccgtgccgtg ggcatcatcg
     3601 accatcaacc agccattgtg ctgttgcgtt acctgctgga tttccgccag tggcgcacta
     3661 tcgccgtcca tgctgaacac gccttctgtc accaccagtt gctgccccgg acagggggaa
     3721 gcaagcagtc gcgccagatg agtgacatcg ttatgaacaa aacggcgaag ctgcgacggg
     3781 cttaaactgg cagcctccag caatgaggca tggctaagcc ggtcagcgac aatgcggtcc
     3841 tcttttgcca tcatcgcggc aatgaccgcc tgattagcgg caaaaccaga gataaatagc
     3901 agtgcccgcg aatagcccag ccactcggcc agttcttctt ccagcgcctg atgcgcaacg
     3961 ctataaccgc tgacgtgacc ggaaccgccg ctaccgacgc caaattgctc cgcaccctgc
     4021 tgccaggcac ggataatttg cggatgatgg cttaaaccta aataatcgtt actggaaaag
     4081 ttcagatact ggcgatcatc cgccaccagc cagcgtccgg ctccttgcgc cactggataa
     4141 cggcgacgca gggcatcggc agcacgccgc gcatcgagcg ccgcgttgat tttctcctgc
     4201 cagctcatag tgctgccgcg ttgtaatatt cgtcagtatc cggggtcatc agcgcctgtt
     4261 caagacgttg ctgttgttcg ttatcccctg ccagcacggc agtttgctgc ggatttagcc
     4321 ccagtttgcg gaacagttgc aggtctttat cttcttccgg attcggcgtg gtcagcagtt
     4381 tgcaaccgta gaaaatcgag tttgcgcctg ccataaagca catcgcctga gtctgttcgt
     4441 tcatctgctc gcgtccggca gaaaggcgca cgtaagaggt cggcatcatg atccgcgcga
     4501 ccgcaatggt gcgaataaaa tcaaaggcat caacgtcatc gttatcggca agcggcgtgc
     4561 ctttcacctt caccagcatg ttgattggca cgctttccgg cggcgtcggc aggtttgcca
     4621 gttgcagcaa taatccggcg cgatctttta ccgtttcgcc taagcccaca atgccgccag
     4681 aacagacttt gatcccggca tcgcgcactt tttccagcgt atcgaggcgt tcctgataag
     4741 tgcgggtggt gatgatattg ccgtaaaact ccggcgaggt gtcgaggttg tggttgtagt
     4801 aatccagccc ggcgttcgcg aggcgctgcg cctgagattc actcaacgtg cccagcgtca
     4861 tacacgcctc cagccctaaa tcttttaccc cctgcaccat ctgctccagg tacggcatat
     4921 cgcgttcgtt gggattcttc cacgccgcgc ccatgcaaaa acgcgtcgat cccgccgctt
     4981 tcgctttgcg cgccgactcc agcacctgtt caacttccat caaccgctcg gcttccagac
     5041 cggttttata gcgcgagctt tgcgggcaat atttgcaatc ttccgggcaa gcaccggttt
     5101 taatcgacag caacgtgctg acctgcacct gacgaggatc gaaatgctgg cgatgcacct
     5161 gctgcgcttc aaacagcaga tccagcaacg gtttttcaaa taattctgcg acttgcgaca
     5221 atgtccagcg tgggcggtga gccatggggc ttctccaaaa cgtgtttttt gttgttaatt
     5281 cggtgtagac ttgtaaacct aaatcttttc aatttggttt acaagtcgat tatgacaaag
     5341 gacgatcttg cctttgacca acgccatatc tggcacccat acacatccat gacctccccg
     5401 ctgccggttt atccggtggt gagcgccgaa ggttgcgagc tgattttgtc tgacggcaga
     5461 cgcctgattg acggtatgtc gtcctggtgg gcggcgatcc acggctacaa tcacccgcag
     5521 cttaatgcgg cgatgaagtc gcaaattgat gccatgtcgc atgtgatgtt tggcggtatc
     5581 acccatgcgc cagccattga gctgtgccgc aaactggtgg cgatgacgcc gcaaccactg
     5641 gaatgcgttt ttctcgcgga ctccggttcc gtagcggtgg aagtggcgat gaaaatggcg
     5701 ttgcagtact ggcaagccaa aggcgaagtg cgccagcgtt ttctgacctt ccgcaatggt
     5761 tatcatggcg atacctttgg cgcgatgtcg gtgtgcgatc cggacaactc aatgcacaac
     5821 ctgtggaaag gctatctgcc agaaaacctg tttgctcccg ccccgcaaag ccgcatggat
     5881 ggtgaatggg atgaacgcga tatggttggc tttgcccgcc tgatggcggc gcatcgtcat
     5941 gaaatcgcgg cggtgatcat tgaaccgatt gtccagggcg caggcgggat gcgcatgtac
     6001 catccggaat ggttaaaacg aatccgcaaa atgtgcgatc gcgaaggtat cttgctgatt
     6061 gccgacgaga tcgccaccgg atttggtcgt accggcaaac tgtttgcctg tgaatatgca
     6121 gaaatcgcgc cggacatttt gtgcctcggt aaagccttaa ccggcggcac aatgaccctt
     6181 tccgccacac tcaccacgcg cgaggttgca gaaaccatca gtaacggcga agccggctgc
     6241 tttatgcacg ggccaacttt tatgggcaat ccgctggcct gcgcggctgc aaacgccagc
     6301 ctggcgattc tcgaatctgg cgactggcag caacaggtgg cggctattga agtacagctg
     6361 cgcgagcaac ttgcccccgc ccgtgatgcc gaaatggttg ccgatgtgcg cgtgctgggg
     6421 gcaatcggcg tagtcgaaac aactcgtccg gtgaatatgg cggcgctgca aaaattcttt
     6481 gtcgaacagg gggtatggat ccggcctttt ggcaaactga tttacctgat gccgccctat
     6541 attattctcc cgcaacagtt gcagcgtctg accgcagcgg ttaaccgtgc ggtgcaggat
     6601 ggaacatttt tttgccaata acgggaagtc agcgtgtggg tttctggcta cactttctgc
     6661 aaactagaaa ggagggttca tgaaactcat cagtaatgat ctgcgcgatg gcgataagct
     6721 gccgcatcgt catgtcttta acggcatggg ttacgatggc gataatattt caccgcatct
     6781 ggcgtgggat gatgttcctg cgggaacgaa aagttttgtt gtcacctgct acgacccgga
     6841 tgcgccaacc ggctccggct ggtggcactg ggtagttgtt aatttacccg ctgatacccg
     6901 cgtattaccg caagggtttg gctctggtct ggtagcaatg ccagacggcg ttttgcagac
     6961 gcgtaccgat tttggtaaaa ccggatacga tggcgcagcg ccgccgaaag gcgaaaccca
     7021 tcgctacatt tttaccgttc acgcgctgga tgtagagcat attgatgtcg atgaaggagc
     7081 cagcggcgcg atggtcgggt ttaacgttca tttccactct ctggcaagcg catcgattac
     7141 cgcgatgttt agttaatcac tctgccagat ggcgcaatgc tatctggtat cacttaaagg
     7201 tattaaaaac aactttttgt ctttttacct tcccgtttcg ctcaagttag tataaaaaag
     7261 caggcttcaa cggattcatt tttctatttc atagcccgga gcaacctgtg aacacatttt
     7321 cagtttcccg tctggcgctg gcattggctt ttagcgtgac gctgaccgcc tgtagctcaa
     7381 caccacccga tcaacgtcct tctgatcaaa ctgcgcctgg tacctcttct cgcccgattc
     7441 tgtcggcaaa agaagcgcag aatttcgatg ctcaacacta ttttgcatcc ctgacaccag
     7501 gtgctgcagc gtggaatcct tccccgatta ccctgcctgc gcaacctgac tttgttgtcg
     7561 gcccggcggg cactcaaggt gtaacgcata ccacgattca ggcggcggta gatgcggcaa
     7621 ttatcaagcg taccaacaag cgccagtata ttgccgtgat gcctggtgag tatcagggaa
     7681 cggtgtatgt ccctgctgct ccgggtggaa ttactctgta cggtacaggt gaaaaaccga
     7741 ttgatgtgaa gattgggctt tcccttgatg ggggcatgag ccctgccgac tggcgtcacg
     7801 acgtcaaccc gcgcggcaaa tatatgccag gtaaaccagc gtggtatatg tacgatagct
     7861 gccagagcaa acgcagcgac agtatcggtg ttctctgctc tgcggtcttc tggtcacaaa
     7921 acaatggcct gcaactgcaa aacctgacca tcgaaaacac gctgggcgat agcgtagatg
     7981 caggtaacca tccggcggtg gcactgcgta ctgatggtga ccaggtacag attaacaacg
     8041 ttaacattct cggtcgtcag aacaccttct ttgtcaccaa cagcggtgtg cagaaccgtc
     8101 tggaaacgaa tcgtcagccg cgtacgctgg tgaccaacag ctacattgaa ggggatgtgg
     8161 atatcgtttc tggtcgcggc gcagtggtgt tcgataacac cgaattccgc gtggtgaact
     8221 cacgtactca gcaagaagcg tatgtgtttg caccggctac gctgtccaac atttactacg
     8281 gtttcctcgc cgtaaacagc cgtttcaatg ctttcggtga tggtgtggcg caactgggcc
     8341 gctcgctgga tgttgatgcc aataccaacg gtcaggtggt gatccgtgat agcgccatca
     8401 acgaaggttt taacacggct aaaccgtggg ccgatgcggt gatctctaat cgtccgtttg
     8461 cgggtaatac cggcagcgta gatgataacg acgaaataca gcgcaatctg aatgacacta
     8521 actacaaccg catgtgggaa tacaataacc gcggcgtggg tagtaaagtg gttgcagagg
     8581 cgaagaagta agagcaatta actatttgcc ggatgcggcg taaacgcctt atccggccta
     8641 cggttcgatg cgatttgtag gtcggataag atgcgcaagc atcgcatccg acaataagtg
     8701 ccgaatgcga cctacattca catggcgctt tttacatctg acggttttta ttgaagttaa
     8761 tcaaactacc cgccttgata atctcgcgct cttcagcagt cagactttcc atatagagcg
     8821 taatttccgt taccggcgca tcttcatgga tcacataacc tttaaacgtc gtacccggat
     8881 tatccagcgc cgctttaatg ccagggatgt aaatgtaatc ccccacttca aaggttggca
     8941 cttccgccat ctgcagcggt aacatccccc agttgatgac gttagaacgg tagcgtttag
     9001 tcgcgtactc ttcggcaata ttcgccagac cgccaatcac acgctggcag ctcgccgcct
     9061 gttcacgcgc agaaccatcg cctggtttca ccgcatatac catgctgcca atttcagttt
     9121 gcagcggatc aatatgctcc tgaccagcaa tctgcttaat gcgcgcaaac acctctgtca
     9181 gctcgctgac attccccgcc agacgctgat tttccagctc agcagtcgct ttacttctgc
     9241 caacataacc gggatcgcgg cgtgacaggg taaactccgc cagaccaatc ggatttgaac
     9301 gataagaaga ggtttcaccg gaaggaatca gttcgtcggt ggtggtcact tcgtcgagga
     9361 tcttcgagca cactttcagg acgatattgt cagtcagcgc acccaattcc ggccagtctt
     9421 taatgttcgg tccgtaaatc agcggttgct gagttgcccc tttcacaaag ccctgataaa
     9481 cacggttttt atacggcgtt acatcgaagg cgtactccgg cacgttgtcc cagcaatcaa
     9541 gttcgctggc agaggttaaa tagccaccgt ttgccgcagt cgcagcgata gaacgagcgt
     9601 ccatcaacgc caccgctgac atctgcccat tagctggctt agagccttcg cggttcggga
     9661 agttacgcgt ggtgtggcga atacttaatc cgttgttgat tggcgtatcg cccgcgccaa
     9721 agcatgggcc gcagaacgcg gttctgatga ttgcgcctgc gccaatcaaa tctgctacca
     9781 cacctttttt ggctaaatcc ataaacaccg gctgtgatga cgggtaaacc gccagcgaga
     9841 aggtgtcatt gccacaggat tgaccgcgca gtgcattcgc cgccgcgatg acgttttcgt
     9901 agttaccgcc agaacagccc gcgataatcc cctgctgcac tttcaggcga ctattttcca
     9961 ctttatccag cagcgagagt ttggctttac cgtgcgccac gcgttcggac tcaatttcaa
    10021 tctcacgcag aatgtcggtc aggttctggt tcagtgtgtc gatttcatac acgttgctcg
    10081 ggtggaacgg cagcgcaatc attggtttga tggcgcttaa atcaacgctg atgcagccat
    10141 cgtagtacgc catcggttga gggttaagct ggcagtaatc ctggccgcga ccgtgcagcg
    10201 ccagccagtt atgcacttct tcatcagttt gccagacgga gcttaaacag gtggtttccg
    10261 tggtcatcac gtcaacgctg ttacggaaat cggtagagag cgcgctaacg cccggtccaa
    10321 cgaactccat gactttgttt ttgacgtaac cgtttttgaa caccgcgcca atgatagcca
    10381 gcgccacatc ctgtggcccc acatacggcg caggttttcc ggtcagatgc accgcaacca
    10441 cgcccggata gtcgatatcc caggtgtcat taagcagctg ttttaccaac tcaccgccgc
    10501 cctcaccgac tgccattgtc cctaatgcac cgtaacgggt gtggctgtct gacccgagga
    10561 tcattttgcc cccgcctgcc atcatctcac gcatatattg atggatgatc gcaatatgcg
    10621 gaggcacaaa aataccgcca taacgctgag ctgccgataa accaaaaaca tggtcatcac
    10681 cgttaatggt gccgccgacg gcgcagagtg agttatggca gttggtcagc acatacggca
    10741 gcgggaaacg ttccattccg gacgctttag cggtctgtac aataccaaca aaggtaatat
    10801 cgtgagaggc taatgaatca aacttaattt taagtttatc catatttccg gacgtattat
    10861 gagaagagag aatagaccag gcaatagtgc cttttttggc ttcttctttt ttaatttcgc
    10921 cggtgaaatg ttcttcggca attatttcgt tattactggc gagaaacacg ccttt
//
LOCUS       DADLTD010000144        10929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLTD010000144 DADLTD010000000
VERSION     DADLTD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.144"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..1072
                     /locus_tag="JN701_002960"
                     /pseudo
     CDS             1..1072
                     /locus_tag="JN701_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            1920..3488
                     /gene="cydA"
                     /locus_tag="JN701_002961"
     CDS             1920..3488
                     /gene="cydA"
                     /locus_tag="JN701_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY7365333.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            3504..4643
                     /gene="cydB"
                     /locus_tag="JN701_002962"
     CDS             3504..4643
                     /gene="cydB"
                     /locus_tag="JN701_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7365334.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            4658..4771
                     /gene="cydX"
                     /locus_tag="JN701_002963"
     CDS             4658..4771
                     /gene="cydX"
                     /locus_tag="JN701_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY7365335.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            4771..5064
                     /gene="ybgE"
                     /locus_tag="JN701_002964"
     CDS             4771..5064
                     /gene="ybgE"
                     /locus_tag="JN701_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY7365336.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            5214..5618
                     /gene="ybgC"
                     /locus_tag="JN701_002965"
     CDS             5214..5618
                     /gene="ybgC"
                     /locus_tag="JN701_002965"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7365337.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            5615..6307
                     /gene="tolQ"
                     /locus_tag="JN701_002966"
     CDS             5615..6307
                     /gene="tolQ"
                     /locus_tag="JN701_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY7365338.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            6311..6739
                     /gene="tolR"
                     /locus_tag="JN701_002967"
     CDS             6311..6739
                     /gene="tolR"
                     /locus_tag="JN701_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY7365339.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            6804..7985
                     /gene="tolA"
                     /locus_tag="JN701_002968"
     CDS             6804..7985
                     /gene="tolA"
                     /locus_tag="JN701_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY7365340.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            8118..9410
                     /gene="tolB"
                     /locus_tag="JN701_002969"
     CDS             8118..9410
                     /gene="tolB"
                     /locus_tag="JN701_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY7365341.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            9445..9966
                     /gene="pal"
                     /locus_tag="JN701_002970"
     CDS             9445..9966
                     /gene="pal"
                     /locus_tag="JN701_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY7365342.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            9976..10767
                     /gene="cpoB"
                     /locus_tag="JN701_002971"
     CDS             9976..10767
                     /gene="cpoB"
                     /locus_tag="JN701_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY7365343.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
BASE COUNT         2716 a         2556 c         2915 g         2742 t
ORIGIN      
        1 aattgaagaa agctctgatg atggtgatga atatgactat tcacccgcaa aagaagagtg
       61 ggtaattacc gcagcgaacg cgaaaccgca atgcgatatt attcatgaag cctggcagag
      121 cagggctgtt atccgctatg acatggcagt gccgctcaat ttgtcagaac gcagcgcccg
      181 gcaatccact ggcagagtag gggtggtgtt ggttgtcact cttagtcata acagcaggcg
      241 tattgatgtg gatatcaacc ttgataacca ggctgacgat catcgccttc gtgtcctggt
      301 ccctacacct tttaacaccg acagtgttct ggcagatacg cagtttggtt cgctaacgcg
      361 ccccgtgaac gacagtgcaa tgaacaactg gcagcaagaa ggctggaaag aagcgccggt
      421 tccggtatgg aatatgctca actatgttgc cttacaggaa gggcgtaacg gcatggctgt
      481 ctttagcgaa gggttacgtg aatttgaagt catcggtgaa gagaagaaaa cctttgccat
      541 tacgttgctg cgtggcgtgg gcttactggg caaagaagat ctgcttttaa ggcctgggcg
      601 gccttcggga attaaaatgc cagtcccgga ctcacaacta cgtggtctgc tttcttgtcg
      661 cctaagttta ttgagttata ccggtacgcc aaccgccgct ggtgtagctc agcaggcgcg
      721 agcatggctg actccagtac agtgttacaa caaaatccca tgggatgcga tgaagctcaa
      781 caaagccgga ttcaacgtgc cggaaagtta tagtttgttg aaaatgcccc cagtgggatg
      841 cctgataagc gcacttaaga aagctgaaga ctgacaagaa gtgattttac ggctgtttaa
      901 tccggctgaa tcagcaacct gtgatgcgac tgttgctttc agtcgcgagg tgatttcttg
      961 ctcagaaacg atgatggatg aacacattac caccgaggaa aatcaaggtt caaatctatc
     1021 ggggcctttt ttacccggcc agtcacggac gttcagttac cggcttgcct gaatagcaat
     1081 caaaccgaag ccacatatgc gcggccagat tgttgacaaa gggcgctttg ttcatgccgg
     1141 atacggcatg aacgctttat tcggtctaca aaagcaggca aattcaatat attgcagaga
     1201 tgatgtagac actggcaagc gtagcgcatc aggtaatttt gcgtttatct tcactctcaa
     1261 gccacgtata tgtggcttta tttttaacaa aataataacc ctgggtgagt taattataat
     1321 ataattataa gttaattaaa tgttaatatc ggcgggggtg gatttatgcc tttattagta
     1381 atcctgaaac tctgcgtcgt attagccagt gaccaaaaaa agaattaagg tcaaccgtgc
     1441 tgtttttgct tcgtctcttt ttatctttaa ttgccaaccg aaactaattt cagccttata
     1501 actcacacat tttaaacata aatgtcacta aagttacctt attgaaacat gattaacata
     1561 atttgtagga attgatattt atcaatgtat aagtcttgga aatgggcatc aaaaagagat
     1621 aaattgttct cgatcaaatt ggctgaaagg cggtaattta gctataaatt gatcaccgtc
     1681 gaaaaatgca aatttgcttc aacaaaaacc tgtttattgt aaggattttg cggcgtaata
     1741 tatacgtggg atcaatttga gtttttatta acatgtttgc aacctttctt tacgccgttt
     1801 ttgtgtgcat tcacatggta tgatgaaagt gttcaaacaa atttctattg gggcatgcgt
     1861 gtgacccttt ctaacggggt tcactctcgg agtcttcatg cgatgagcaa ggagtcatga
     1921 tgttagatat agtcgaactg tcgcgcttac agtttgcctt gaccgcgatg taccacttcc
     1981 tttttgtgcc actgacgctc ggtatggcgt tcctgctggc cattatggaa acggtctacg
     2041 tcctctccgg caaacagatt tataaagata tgaccaagtt ctggggcaag ttgtttggta
     2101 tcaacttcgc tctgggtgtg gctaccggtc tgaccatgga gttccagttc gggactaact
     2161 ggtcttacta ttcccactat gtaggggata tcttcggtgc gccgctggca atcgaaggtc
     2221 tgatggcctt cttcctcgaa tccacctttg taggtctgtt cttcttcggt tgggatcgtc
     2281 tgggtaaagt tcagcatatg tgtgtcacct ggctggtggc gctcggttca aacctgtccg
     2341 cactgtggat tctggttgcg aacggctgga tgcaaaaccc aatcgcgtcc gatttcaact
     2401 ttgaaactat gcgtatggag atggtgagct tctccgagct ggtgcttaac ccggttgctc
     2461 aggtgaaatt cgttcacact gtagcgtctg gttatgtgac tggcgcgatg ttcatcctcg
     2521 gtatcagcgc atggtatatg ctgaaaggtc gtgacttcgc cttcgctaaa cgctcctttg
     2581 ctatcgctgc cagcttcggt atggctgctg ttctgtctgt tattgttctg ggtgatgaat
     2641 ccggctacga aatgggcgac gtgcagaaaa ccaaactggc tgctattgaa gccgagtggg
     2701 aaacgcaacc tgcgcctgct gcctttactc tgttcggcat tcctgatcag gaagaggaga
     2761 cgaacaaatt tgcgattcag atcccttacg cactgggcat cattgcaacg cgttccgtgg
     2821 ataccccggt tatcggcctg aaagagctga tggtgcagca tgaagaacgc attcgtaacg
     2881 ggatgaaggc gtactctctg ctcgaacaac tgcgttctgg ttctaccgac caggcggttc
     2941 gtgaccagtt caatagcatg aagaaagacc tcggttacgg tctgctgctg aaacgctata
     3001 cgccaaacgt ggctgatgcg actgaagcgc agattcaaca ggcaactaaa gactccattc
     3061 cgcgtgtagc gccgctgtac ttcgcgttcc gtattatggt ggcgtgtggc ttcctgctgc
     3121 tggcaatcat cgcgctctct ttctggagtg tcatccgcaa ccgcattggc gagaaaaaat
     3181 ggctgctgcg cgccgcgctg tacggtattc cgctgccgtg gattgctgta gaagcgggct
     3241 ggttcgtggc tgaatatggc cgccaaccgt gggctatcgg tgaagtgctg ccgacagctg
     3301 tggcgaattc gtcactgacc gcaggcgatc tcatcttctc aatggtgctg atttgcggcc
     3361 tgtataccct gttcctggtg gcagaattgt tcttaatgtt caaatttgca cgcctcggcc
     3421 caagcagcct gaaaaccggt cgctatcact ttgagcagtc ttccacgact actcagccgg
     3481 cacgctaaga caggagtcgt caaatgatcg attatgaagt attgcgtttt atctggtggc
     3541 tgctggttgg cgttctgctg attggttttg cagtcactga cggtttcgac atgggggtgg
     3601 gcatgctcac ccgtttcctc ggtcgtaacg acaccgagcg tcgaattatg attaactcca
     3661 ttgcaccaca ctgggacggt aaccaggttt ggctgatcac cgcgggcggc gcactctttg
     3721 ctgcctggcc gatggtctat gccgctgcgt tctccggctt ctatgtggcg atgatcctcg
     3781 tgctggcgtc tttgttcttc cgtccggtcg gttttgacta ccgctccaag attgaagaaa
     3841 cccgctggcg taacatgtgg gactggggca tcttcattgg tagcttcgtt ccgccgctgg
     3901 taattggtgt tgcgttcggt aacctgttgc agggcgtacc gttcaacgtt gatgaatatc
     3961 tgcgtctgta ctacaccggt aacttcttcc agttgcttaa cccgttcggc ctgctggcag
     4021 gcgtggtgag cgtagggatg atcattactc agggcgcaac ctatctgcaa atgcgtaccg
     4081 tgggcgaact gcacctgcgt acccgtgcaa cggctcaggt ggctgcgctg gtgacactgg
     4141 tctgtttcgc actggctggc gtatgggtga tgtacggtat cgatggttat gtcgtgaaat
     4201 cgacaatgga ccattacgca gcctctaacc cactgaataa agaagtggtt cgtgaagctg
     4261 gcgcatggct ggttaacttc aacaacacgc caattctgtg ggctattccg gcactgggtg
     4321 tggttctgcc gctgctgacc atcctgactg cacgtatgga taaagccgcg tgggcgtttg
     4381 tgttctcctc cctgacgctg gcctgcatca tcctgacagc cggtatcgca atgttcccgt
     4441 ttgtgatgcc gtccagcacc atgatgaacg caagtctgac aatgtgggat gcaacttcca
     4501 gccagctgac gcttaacgtc atgacctggg ttgcggtggt tctggtaccg atcattctgc
     4561 tctacaccgc ctggtgttac tggaaaatgt tcggtcgtat caccaaagaa gatattgaac
     4621 gtaacaccca ctctctgtac taagtaagga gctaaaaatg tggtatttcg catggattct
     4681 gggaacgctt cttgcctgtt cgtttggggt aatcaccgcg ctggcgcttg aacacgtcga
     4741 atcaggcaaa gccggtcaag aagacatctg atgagtaaga ttatcgcgac cttgtatgcg
     4801 gtaatggaca agcgccccct gcgggcgctt tccttcgtga tggcgcttct gttagcagga
     4861 tgtatgtttt gggacccatc tcgttttgcc gcgaagacca gtgaactgga aatctggcat
     4921 ggtttattgc tgatgtgggc cgtctgtgct ggtgtgattc acggcgtggg ctttcgtccg
     4981 cagaaggttc tttggcaagg gattttttgc ccattgcttg ccgatattgt tctcattatc
     5041 gggctgattt tcttcttctt ttaaatcaga attctcttta aaaaattatg ggccgctcca
     5101 ggcccataaa tttttacgct cccttaactt gccctcattc ccaaacctca atcgcgcgcg
     5161 tatagtagca gcgtttaaaa gctctaactt ttgttgcatt accgggatgt aaagtgaata
     5221 caacgctgtt tcgatggccg gctcgcgtct actatgaaga taccgatgcc ggtggtgtgg
     5281 tgtaccacgc cagttacgtc gctttttatg aaagagcacg cacagagatg ctgcgtcatc
     5341 atcacttcag tcagcaggcg ctgatggctg aacgcgttgc ctttgtggta cgtaaaatga
     5401 cggtggaata ttacgcacct gcgcggctcg acgatatgct cgaaatacag actgaaataa
     5461 catcaatgcg tggcacctct ttggttttca cgcaacgtat tgtcaacgcc gagaatactt
     5521 tgctgaatga agcagaggtt ctggttgttt gcgttgaccc actcaaaatg aagcctcgtg
     5581 cgcttcccaa gtctattgtc gcggagttta agcagtgact gacatgaata tccttgattt
     5641 gttcctgaag gctagccttc tggttaaact tatcatgttg attttgattg gtttttcaat
     5701 cgcatcctgg gccattatta tccagcggac ccgtattctt aacgcagcgg cgcgcgaagc
     5761 cgaagcgttt gaagataaat tctggtctgg aatcgaactc tctcgcctct atcaagagag
     5821 tcaggggaaa cgggataatc tgactggttc ggaacaaata ttttacagcg ggttcaaaga
     5881 gtttgtgcgc ctgcatcgtg ccaatagcca tgcgccggaa gccgtagtgg aaggggcgtc
     5941 gcgtgctatg cgtatctcca tgaaccgtga acttgaaaat ctggaaacgc acattccgtt
     6001 cctcggtacg gttggctcca tcagcccgta tattggtctg tttggtacgg tctgggggat
     6061 catgcatgcc tttatcgccc tcggggcggt aaaacaagca acactgcaaa tggttgcgcc
     6121 cggtatcgca gaagcgttga ttgcgactgc aattggtctg tttgccgcta tcccggcagt
     6181 tatggcctac aaccgcctca accagcgcgt aaacaaactg gaactgaatt acgacaactt
     6241 tatggaagag tttaccgcga ttctgcaccg ccaggcgttt accgttagcg agagcaataa
     6301 ggggtaagtc atggccagag cgcgtggacg aggtcgtcgc gaacttaagt ccgaaatcaa
     6361 cattgtaccg ttgctggacg tactgctggt gctgttgctg atcttcatgg cgacagcgcc
     6421 catcatcacc cagagcgtgg aggtcgatct gccagacgct actgaatcac aggcggtgag
     6481 cagtaacgat aatccgccag tgattgttga agtgtctggt attggtcagt acaccgtggt
     6541 ggttgagaaa gatcgcctgg agcgtttacc accagagcag gtggtggcgg aagtgtccag
     6601 ccgcttcaag gccaacccga aaacggtctt tctgatcggt ggcgcaaaag atgtgcctta
     6661 cgatgaaata attaaagcac tgaacttgtt acatagtgcg ggtgtgaaat cggttggttt
     6721 aatgacgcag cctatctaaa catctgcgtt tcccttgctt gaaagagagc gggtaacagg
     6781 cgaacagttt ttggaaaccg agagtgtcaa aggcaaccga acaaaacgac aagctcaagc
     6841 gggcgataat tatttcagca gtgctgcatg tcatcttatt tgcggcgctg atctggagtt
     6901 cgttcgatga gaatatagaa gcttcagccg gaggcggcgg tggttcgtcc atcgacgctg
     6961 tcatggttga ttcaggtgcg gtagttgagc agtacaaacg catgcaaagc caggaatcaa
     7021 gcgcgaagcg ttctgatgaa cagcgcaaga tgaaggaaca gcaggctgct gaagaactgc
     7081 gtgagaaaca agcggctgaa caggaacgcc tgaagcaact tgagaaagag cggttagcgg
     7141 ctcaggagca gaaaaagcag gctgaagaag ccgcaaaaca ggccgagtta aagcagaagc
     7201 aagctgaaga ggcggcagcg aaagcggcgg cagatgctaa agcgaaggcc gaagcagatg
     7261 ctaaagctgc ggaagaagca gcgaagaaag cggctgcaga cgcaaagaaa aaagcagaag
     7321 cagaagccgc caaagccgca gcgcagaaaa aagccgaggc agccgctgcg gcactgaaga
     7381 agaaagcgga agcggcagaa gcagctgcag ctgaagcaag aaagaaagcg gcaactgaag
     7441 ctgctgaaaa agccaaagca gaagctgaga agaaagcggc tgctgaaaag gctgcagctg
     7501 ataagaaagc tgcagccgac aaaaaagcag cagaaaaagc ggctgctgaa aaggcagcag
     7561 ctgataagaa agcagcggca gaaaaagccg ccgcagaggc agatgatatt ttcggtgagc
     7621 taagctctgg taagaatgca ccgaaaacgg ggggaggggc gaaagggaac aatgcttcgc
     7681 ctgccgggag tggtaatact aaaaacaatg gcgcatcagg ggccgatatc aataactatg
     7741 ccgggcagat taaatctgct atcgaaagta agttctatga cgcatcgtcc tatgcaggca
     7801 aaacctgtac gctgcgcata aaactggcac ccgatggtat gttactggat atcaaacctg
     7861 aaggtggcga tcccgcactt tgtcaggctg cgttggcagc agctaaactt gcgaagatcc
     7921 cgaaaccacc aagccaggca gtatatgaag tgttcaaaaa cgcgccattg gacttcaaac
     7981 cgtaatcgcg atgttgactg ttcggaaggt caacatcagg caccggttgc cacggggttc
     8041 tggtagtttt gtgtatttta gtttgttaac attctgctaa attatcgtgg gccatcggtc
     8101 cagataaggg agatatgatg aagcaggcat tacgagtagc atttggtttt ctcatactgt
     8161 gggcatcagt tctgcatgct gaagtccgca ttgtgatcga cagcggtgta gattccggtc
     8221 gtcctattgg tgttgttcct ttccagtggg cggggcctgg tgcggcacct gaagatattg
     8281 gcggcatcgt tgctgctgac ttgcgtaaca gcggtaaatt taatccgtta gatcgcgctc
     8341 gtctgccaca gcagccgggt agtgcgcagg aagtacaacc atctgcatgg tccgcactgg
     8401 gcattgacgc tgtagttgtc ggtcaggtca ctccgaatcc ggatggttct tacaatgttg
     8461 cttatcaact tgttgacact ggcggcgcac cgggtactgt acttgctcag aactcgtaca
     8521 aagtgaacaa gcagtggctg cgttatgctg gtcataccgc cagtgatgaa gtgtttgaaa
     8581 aactgaccgg cattaaaggt gcgttccgta cccgtattgc ctacgttgtt cagaccaacg
     8641 gcggtcagtt cccgtatgaa ctgcgcgtat ctgactatga cggttacaac cagtttgtcg
     8701 ttcaccgttc accgcagccg ctgatgtctc cggcgtggtc accagacggt tctaaactgg
     8761 cttatgtgac cttcgaaagc ggtcgttccg cgctggttat tcagacgctg gcaaatggcg
     8821 ctgtacgtca ggtggcttca ttcccgcgtc acaacggtgc acctgcattc tcgccagacg
     8881 gcagcaaact ggcattcgcc ttgtcgaaaa ccggtagtct gaacctgtac gtaatggatt
     8941 tggcttctgg tcagatccgc caggtgactg atggtcgcag taacaatacc gaaccgacct
     9001 ggttcccgga tagccagaac ctggcattta cttctgacca ggccggtcgt ccgcaggttt
     9061 ataaagtgaa tatcaacggc ggtgcgccac aacgtattac ctgggaaggt tcgcagaacc
     9121 aggatgcgga tgtcagcagc gacggtaaat ttatggtaat ggtcagctcc aatggtgggc
     9181 agcagcacat tgccaaacaa gatctggcaa cgggaggcgt acaagttctg tcgtccacgt
     9241 tcctggatga aacgccaagt ctggcaccta atggcactat ggtaatctac agctcttctc
     9301 aggggatggg atccgtgctg aatttggttt ctacagatgg gcgtttcaaa gcgcgtcttc
     9361 cggcaactga tggacaggtc aaattccctg cctggtcgcc gtatctgtga taataattaa
     9421 ttgaatagta aaggaatcat tgaaatgcaa ctgaacaaag tgctgaaagg gctgatgatt
     9481 gctctgcctg ttatggcaat tgcggcatgt tcttccaaca agaacgccag caatgacggc
     9541 agcgaaggca tgctgggtgc cggcactggt atggatgcga acggcggcaa cggcaacatg
     9601 tcttccgaag agcaggctcg tctgcaaatg caacagctgc agcagaacaa catcgtttac
     9661 ttcgatctgg acaagtacga tatccgttct gacttcgctc aaatgctgga tgcacatgca
     9721 aacttcctgc gtagcaaccc gtcttacaaa gtcaccgtag aaggtcacgc ggacgaacgt
     9781 ggtactccgg aatacaacat ctccctgggt gaacgtcgtg cgaacgccgt taagatgtac
     9841 ctgcagggta aaggcgtttc tgcagaccag atctccatcg tttcttacgg taaagaaaaa
     9901 cctgcagtac tgggtcatga cgaagcggca tactccaaaa accgtcgtgc ggtactggtt
     9961 tactaagaga attgcatgag cagtaacttc agacatcaac tattgagtct gtcgttactg
    10021 gttggtatag cggccccctg ggccgctttt gctcaggcac caatcagtag tgtcggctca
    10081 ggctcggtcg aagaccgcgt cactcaactt gagcgtattt ctaatgctca cagccagctt
    10141 ttaacccaac tccagcaaca actttctgat aatcaatccg atattgattc cctgcgtggt
    10201 cagattcagg aaaatcagta tcaactgaat caggtcgtgg agcggcaaaa gcagatcctg
    10261 ttgcagatcg acagcctcag cagcggtggt gcagcggcgc aatcaaccag cagcgatcaa
    10321 agcggtgcgg cggcatcaac gacgccgaca gctgatgctg gtactgcgaa tgctggcgcg
    10381 ccggtgaaaa gcggcgatgc aaacacggat tacaatgcag ctattgcgct ggtgcaggat
    10441 aaatcccgcc aggatgacgc aatggtggca tttcagaatt tcatcaaaaa ttaccctgat
    10501 tcaacttacc tgccaaacgc caattattgg ctgggtcagt taaactacaa caagggtaaa
    10561 aaagatgatg cggcgtacta ttttgcttcg gtagtgaaaa actatccgaa gtcaccaaag
    10621 gctgctgatg cgatgtttaa agtcggcgtc atcatgcagg acaaaggtga caccgcaaaa
    10681 gcgaaagccg tataccagca ggttatcagt aaataccctg gtactgatgg cgctaaacag
    10741 gcacaaaaac gtctgaacgc gatgtaatgc ataacacacg accagaagtt gcattatttc
    10801 tggtcgtgtc gtgcgaatca taagcagttg agtgatctgc atcgaaattt ttgttgcgct
    10861 caagtctgaa atcagtaata tatgccgccg ttgccacggg atatcaaaca aaccgaaagc
    10921 aacgaaaaa
//
LOCUS       DADLTD010000145        10903 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLTD010000145 DADLTD010000000
VERSION     DADLTD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.145"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            14..184
                     /locus_tag="JN701_002972"
     CDS             14..184
                     /locus_tag="JN701_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene biosynthesis protein"
                     /protein_id="HAY7365344.1"
                     /translation="MLQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLH
                     NSCIIKICSICY"
     gene            complement(252..1502)
                     /gene="icd"
                     /locus_tag="JN701_002973"
     CDS             complement(252..1502)
                     /gene="icd"
                     /locus_tag="JN701_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY7365345.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWQKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1674..2327
                     /gene="rluE"
                     /locus_tag="JN701_002974"
     CDS             1674..2327
                     /gene="rluE"
                     /locus_tag="JN701_002974"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY7365346.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2337..2798
                     /gene="nudJ"
                     /locus_tag="JN701_002975"
     CDS             2337..2798
                     /gene="nudJ"
                     /locus_tag="JN701_002975"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY7365347.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2852..3958
                     /gene="mnmA"
                     /locus_tag="JN701_002976"
     CDS             2852..3958
                     /gene="mnmA"
                     /locus_tag="JN701_002976"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY7365348.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3994..4635
                     /gene="hflD"
                     /locus_tag="JN701_002977"
     CDS             3994..4635
                     /gene="hflD"
                     /locus_tag="JN701_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY7365349.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4639..6009
                     /gene="purB"
                     /locus_tag="JN701_002978"
     CDS             4639..6009
                     /gene="purB"
                     /locus_tag="JN701_002978"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY7365350.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6177..6848
                     /gene="phoP"
                     /locus_tag="JN701_002979"
     CDS             6177..6848
                     /gene="phoP"
                     /locus_tag="JN701_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY7365351.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6848..8308
                     /gene="phoQ"
                     /locus_tag="JN701_002980"
     CDS             6848..8308
                     /gene="phoQ"
                     /locus_tag="JN701_002980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY7365352.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8384..9505
                     /locus_tag="JN701_002981"
     CDS             8384..9505
                     /locus_tag="JN701_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7365353.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9554..10780)
                     /gene="pepT"
                     /locus_tag="JN701_002982"
     CDS             complement(9554..10780)
                     /gene="pepT"
                     /locus_tag="JN701_002982"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY7365354.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMH
                     YIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
BASE COUNT         2695 a         2787 c         2727 g         2694 t
ORIGIN      
        1 taagcttggt attatccttc aggaaaaaga atatcctatt actatttata acattgcacc
       61 attcaacaag aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag
      121 caaaataaca aataaatgtt tacataatag ttgcattata aaaatatgct cgatatgcta
      181 ttaataatat ccacataaat aaaacaacgg gcgtgttata cgcccgtttc aatatttaac
      241 tcatggagag attacatgtt ttcgatgatc gcttcaccaa actctgaaca tttcagcagc
      301 ttagcgcctt ccatcagacg ttcaaagtca taagttacgg ttttcgcgtt gatagcacct
      361 tccatacctt taacaattaa atcagccgct tcagtccacc ccatatggcg cagcatcatc
      421 tcagcggaga ggatgataga gcctggattt actttgtcct gaccggcgta tttcggcgca
      481 gtaccgtggg tggcttcaaa cagggcgcat tcgtcaccga tgtttgctcc aggagcgata
      541 ccgataccgc caacctgcgc tgccagggcg tcagaaatgt agtcaccgtt caggttcata
      601 caggcgataa catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca
      661 atcacgtctt taatgacgat ctctttgccg gtgttcgggt ttttaacttt ctgccacggg
      721 ccgccgtcga tcagttcacc gccaaactct tcacgcgcca gctggtagcc ccagtcttta
      781 aacgcgcctt cggtgaactt catgatgttg cctttgtgca ccagagtcac agagtcacga
      841 tcgttagcaa ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttccgaa
      901 cacggcttaa taccgatacc acaatgttcc gggaagcgaa ttttcttcac ccccatctct
      961 tcacgcagga atttaatcac tttctcggcg tcggcagagt ctgctttcca ttcgataccc
     1021 gcataaatgt cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta
     1081 accgggcttg gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt
     1141 tcctggcgca gggcaacgtt cagagagcga ataccgccac caacaggagt ggtcagcgga
     1201 cctttaatgg caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca
     1261 tcctgaccat aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta
     1321 cgctcgcctt tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt
     1381 acatctacac cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg
     1441 agtttgccgt tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc
     1501 attcacctct ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt
     1561 tgcgtcctgc gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg
     1621 tcaatactac cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc
     1681 aattcataat ctctgaaaat accatgcaaa aaacttcttt tagaaatcac ctggttaagc
     1741 gattcagctc gcaacgttct accaggcgta aacctgaaaa ccagcccacg cgtgtgatcc
     1801 tgttcaataa accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat
     1861 taaaagaatt catcccggtt cagggcgttt atgcagcagg tcgtcttgac cgcgatagcg
     1921 aagggttact ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggca
     1981 aacgcaccgg gaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg
     2041 aagccttgcg caatggcgta accttaaatg atggccctac cctgcccgcc ggcgcggagc
     2101 tggttgacga accagcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta
     2161 ttcccaccag ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca
     2221 tgaccgccca tgttggtttc cccacgctgc gactgattcg ttatgcgatg ggtgattaca
     2281 ctttggataa ccttgccaat ggcgaatggc gagaagcaac agaataagga tgcacaatgt
     2341 ttaaaccaca cgttaccgtt gcttgcgtag tgcacgcaga gggtaaattt ttagtcgttg
     2401 aagagacgat taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg
     2461 aaaccttagt ggaagctgcc gcccgtgagc tatgggaaga aaccggcatc agcgcgcaac
     2521 cgcaacactt tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt
     2581 tcctctttgc cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg
     2641 actgctgtcg ttgggtcagc gccgaagaaa ttttaaaggc gtcaaatctt cgttcgccgc
     2701 tggtggcgga aagtatccgt tgttatcaaa gcgggcaacg ctatccgctg gagatgattg
     2761 gtgattttaa ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc
     2821 cgccttgaag ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt
     2881 cggcatgtcc ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata
     2941 tcaggttgaa ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac
     3001 agcggcagcg gatctggctg atgcccaagc tgtctgcgac aagctcggca ttgaactgca
     3061 caccgttaac tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata
     3121 taaagccggt cgcacgccga atccggatat tctgtgcaac aaagagatca aatttaaagc
     3181 tttcctcgaa tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt
     3241 acgtcgggcc gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga
     3301 ccagagctac ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt
     3361 cggcgaactg gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc
     3421 gaagaaaaaa gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttcct
     3481 gggccgttat ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg
     3541 tgagcaccag gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg
     3601 caccaaagaa ggcaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat
     3661 tctggttgtt gcgcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca
     3721 gcagttgcac tgggtcgatc gcgaaccatt caccggtact atgcgttgca cggtaaaaac
     3781 ccgctatcgc cagaccgata tcccttgcac cgtcaaggcg ctggacgatg atcgcattga
     3841 agtgattttc gatgaaccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa
     3901 cggtgaagtg tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt
     3961 attatcttta cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc
     4021 ctcgccctgg ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg
     4081 cattgtgatg ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc
     4141 tcgacgctgg cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg
     4201 ctcggcgtgc tcaatgccag cagtcgccag ggcttaaacg ccgaattaac ccgctacaca
     4261 ctcagcttga tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg
     4321 ggcaaccgga tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg
     4381 ctgatgagcg cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt
     4441 caggtcaccg gttcccctgc tgtactgcaa agcccacaag tgccggcgaa agttcgcgca
     4501 accctgctgg caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtctg
     4561 caactgatgt tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta
     4621 accccggagt tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga
     4681 cgctacggcg ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa
     4741 ttccgtgtac aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag
     4801 gaagttcctg cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc
     4861 agcgaagaag atgccgcacg catcaaaacc atcgagcgta ccactaacca cgacgttaaa
     4921 gcggttgagt atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct
     4981 gaattcatcc actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg
     5041 ctgaaaaccg cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt
     5101 aaagatctcg ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca
     5161 gccacgccgt caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggagcgccag
     5221 taccgccagc ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat
     5281 aacgcccaca tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc
     5341 acctcgctgg gtattcagtg gaacccgtac actacccaga ttgaaccgca cgactacatt
     5401 gccgaactgt ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac
     5461 gtctggggtt atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt
     5521 tcttccacca tgccgcataa agttaacccg atcgacttcg agaactccga agggaatctg
     5581 ggcctttcca acgcggtatt acagcatctg gcaagcaaac tgccggtttc ccgctggcag
     5641 cgtgacctga ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg
     5701 attgcgtatc aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg
     5761 ctggatgagc tggatcacaa ctgggaagtg ctggctgagc caatccagac agttatgcgt
     5821 cgctatggca tcgaaaaacc gtacgagaag ctgaaagagc tgactcgtgg taagcgcgtt
     5881 gacgccgaag gcatgaagca gtttatcgac ggtctggcgt tgccagaaga agagaaagcc
     5941 cgcctgaaag cgatgacgcc ggcaaactac attggtcgcg ccatcaccat ggttgatgag
     6001 ctgaaataaa ctcgtatcag tgccggatgg cgatgctgtc cggcctgctt attaagatta
     6061 tccgcttttt attttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc
     6121 cataaccaca taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc
     6181 gcgtactggt tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg
     6241 atgctggtca tcaggtcgat gatgcagaag atgccaaaga agccgattat tatctcaatg
     6301 aacatttacc ggatattgcg attgtcgatc tcggattgcc agacgaggac ggtctgtcac
     6361 tgattcgccg ctggcgtagc aatgatgttt cactgccgat tctggtatta accgcccgtg
     6421 aaagctggca ggacaaagtc gaagtattaa gtgctggtgc tgatgattac gtgactaaac
     6481 catttcatat tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc
     6541 tggcttcaca ggtcatttcg ctaccaccgt ttcaggttga tctctctcgc cgtgaattat
     6601 ctattaatga cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga
     6661 tacgcaataa tggcaaagtg gtgagcaaag attcgttaat gctccaactc tatccggatg
     6721 cggagctgcg ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc
     6781 aggcacaata tccgcaagaa gtgattacta ccgttcgcgg ccagggctat ttgttcgaat
     6841 tgcgctgatg aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct
     6901 gttggcaacg gctgcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat
     6961 cggttatagc gtcagtttcg ataaaactac gtttcggctg ttacgtggcg agagcaatct
     7021 gttctatacc cttgcgaagt gggaaaacaa taagttgcat gtcgagttac ccgaaaatat
     7081 cgacaagcaa agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc
     7141 gcaacgtgac gtgccctggc tgatgaagat gatccagcct gactggctga aatcgaatgg
     7201 ttttcatgaa attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc
     7261 gatacagcaa cagttgcagg aagtgcggga agatgatgac gacgcggaga tgacccactc
     7321 ggtggcagta aacgtctacc cggcaacatc gcggatgcca aaattaacca ttgtggtggt
     7381 ggataccatt ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt
     7441 gctctcagcc aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt
     7501 acgccccatc gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt
     7561 gctcaatcca gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt
     7621 aaaaagtgaa cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag
     7681 tctgaaaacg ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat
     7741 gagcgtcagt gatgctgagc cggtaatgct ggagcaaatc agccgcattt cacagcaaat
     7801 tggctactac ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca
     7861 tccggtcgcc ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa
     7921 aggggtcaat atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga
     7981 ttttgtcgag gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt
     8041 cgaaatttct gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc
     8101 cggtattcca ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt
     8161 acgccctggg caaggtgtag ggctggcggt agcccgcgaa atcaccgagc aatatgaggg
     8221 taaaatcgtc gccggagaga gcatgctggg cggtgcgcgg atggaggtga tttttggtcg
     8281 ccagcattct gcgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca
     8341 tccgttataa tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac
     8401 tcttaactgg cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg
     8461 cggctttaat aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga
     8521 aagcgaagtt gacagtcgac tggtcagtca ccaggatggc aaatggcagg tcagccacgg
     8581 cccgttcgaa agctacgatc atctcggtga aaccaactgg tcattactgg tacaggcagt
     8641 gaaccactgg catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg
     8701 gcgtattgat gatctgatga tttctttttc tgtacctggc ggcggcgtcg gcccgcatct
     8761 cgatcagtac gacgtgttta tcattcaggg taccggacgt cgtcgctggc gagtgggcga
     8821 aaagctgcaa atgaaacagc actgcccaca tccggatctg ttacaggtcg atccgttcga
     8881 agccatcatc gatgaagagc tggagcctgg tgatattctt tatattccgc caggattccc
     8941 gcatgaaggc tacgcgctgg aaaatgcgat gaactattcc gtgggctttc gcgcgccaaa
     9001 tacgcgggaa ctgattagtg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa
     9061 ctactacagc gatcctgatg ttccacctcg cgctcatcct gcggatgttc tgcctcaaga
     9121 gatggataaa ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca
     9181 atggtttggc gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccggaacc
     9241 gccttatcag ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct
     9301 gggtggtctg cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga
     9361 ttccccgcac cgtccggcac tggatgcact cgccagcaac attgcgctga ctgcggagaa
     9421 ttttggcgat gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag
     9481 cgggtattgg ttcttcgaag ggtaagtttg gatttaagcc ggatgcggca tcacatgccg
     9541 catccctttt cgcttacttc cgttgcgccg ttaactcggc aatacggacg atcacctgca
     9601 ccgctttttc cataccttcc agagtcacaa actcatgctt accatgatag ttgtaaccgc
     9661 cagtgaacaa gttcgggcac ggtaatccca taaacgacaa ctgcgcgccg tcggtaccgc
     9721 cgcggatcgg tttcagttcc ggttcaatat cgcagtcacg cattgcctgc tgggcgatat
     9781 cgagaatatg cggatgctca accactttct cgcgcatatt gtagtaactg tcttcaatca
     9841 ccagttcaat gtagcaatca ggatgtaacc ctttgcccac ttttttggcg atctccatca
     9901 ttttacattt acgcgcttca aactgtttac ggtcgaaatc acggatgatg tagtgcatat
     9961 cggcccgttc aacggtgcct ttcatgcccg ccaggtgata gaaaccttca tagccttctg
    10021 tcatttccgg gctttcatcc gccggaactt ccgcatgaat acgtgccgcc agcgacagcg
    10081 catttaccat cactcctttc gccgtgcccg gatgaacatt gttaccgaca attttgatat
    10141 tgaccgatgc ggcgttgaag ttttcaaact ccagttcgcc tacgccacca ccgtcaacag
    10201 tgtaagccca gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct ttgcccactt
    10261 cttcatccgg ggtaaaggcg acgcgaatat caccatgcgg aatgtttttc tgttgcaata
    10321 ccgccagcgc ggtcatgatt tctgcaatac ctgctttgtc atcggcacct aacaaggttt
    10381 taccatcggt ggtaatcaat gtctgaccca gtagctgatg cagcaccggg aacataaccg
    10441 gtgataaaac ttcatcgccg atacccagcg caatatcgcc accgcgatag ttttcaacaa
    10501 tttgcggatt aacatttttg ccgctgcaat ccggtgaggt atccacatga gaaataaagc
    10561 caatcgccgg gatatcgcca gggacgttag ccggtaacgt cgccatcaaa gtgcccttct
    10621 cacttaaggt cacattgata agccccattt cttcgagctg ctctttcagc agatgcaata
    10681 acttccattg gccttccgtg ctgggaacct gtctcacccc tgcttttgat tgggtatcca
    10741 gagacacgta gttcaaaaat cgctcaagta gtttatccat gtagtcaccc tcactttttg
    10801 tgacaacatt attaagaagc aagaaaagac aaatattgcg tcaggtcact tttacccctg
    10861 caagcgggaa tatttatcag cattacctta atgaatataa agc
//
LOCUS       DADLTD010000146        10897 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLTD010000146 DADLTD010000000
VERSION     DADLTD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.146"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(228..2087)
                     /gene="gss"
                     /locus_tag="JN701_002983"
     CDS             complement(228..2087)
                     /gene="gss"
                     /locus_tag="JN701_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY7365355.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2292..3158
                     /gene="yghU"
                     /locus_tag="JN701_002984"
     CDS             2292..3158
                     /gene="yghU"
                     /locus_tag="JN701_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY7365356.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="JN701_002985"
     CDS             complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="JN701_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY7365357.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="JN701_002986"
     CDS             complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="JN701_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7365358.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="JN701_002987"
     CDS             complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="JN701_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY7365359.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4357..4851)
                     /locus_tag="JN701_002988"
     CDS             complement(4357..4851)
                     /locus_tag="JN701_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY7365360.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="JN701_002989"
     CDS             complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="JN701_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY7365361.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="JN701_002990"
     CDS             complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="JN701_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY7365362.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="JN701_002991"
     CDS             complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="JN701_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY7365363.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="JN701_002992"
     CDS             complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="JN701_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY7365364.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10015..10302)
                     /locus_tag="JN701_002993"
     CDS             complement(10015..10302)
                     /locus_tag="JN701_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY7365365.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10421..10897)
                     /locus_tag="JN701_002994"
                     /pseudo
     CDS             complement(10421..10897)
                     /locus_tag="JN701_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
BASE COUNT         2659 a         2989 c         2692 g         2557 t
ORIGIN      
        1 caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt atcagacaat
       61 aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact ggttaatata
      121 ttcacatatg aaatgaataa atattagaac atataaatat tgaatatttt gattaatccc
      181 cgtactgatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
      241 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
      301 cacgtcccac cgtagttgcc gcccacggtg aaggtacata cctgaatgta tttaccgtcc
      361 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
      421 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
      481 gcaatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      541 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      601 ccggggatca ccgtccacag cggctcaaag actagcactt ccgggcgcag caatacgtcg
      661 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
      721 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      781 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      841 aatccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      901 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      961 ctgtgtttcc aggcaccagc caattcgtta atcagccctt ccgccggatt gaagccgttg
     1021 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
     1081 gcggagtcgg cgttgtactc gtaaaccttc aggccacgct catccatgca gaaatccata
     1141 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1201 attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagtacctt gtcggttgcg
     1261 tgaagataca tcaggtgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
     1321 gtaatggtgt agtaatgata aggatcctga ttgatcacct gaccgttggc ctgcacatag
     1381 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1441 aggcgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1501 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtctttc
     1561 agggtatagc agccgttttc gaccaccatc tccagctcac gcgtccactg ttgcccttgc
     1621 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1681 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1741 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1801 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1861 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
     1921 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1981 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     2041 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     2101 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     2161 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2221 aaaattcaca ttctgtacag caagtgacct gccacactgc ttcaacacta ccactcagaa
     2281 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2341 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2401 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2461 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
     2521 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2581 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc tgtgtgtttg
     2641 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2701 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2761 tcggcggtgg ttttggtcac ttttaccatt acgcaccggt aaagattgag tacgccatca
     2821 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     2881 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2941 gcaacgtggt gttaggtggt gtgtatgatg ccgctgagtt tcttgatgcg ggcagttata
     3001 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     3061 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     3121 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     3181 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     3241 cgacgctgcc gcgtcttatc aggcctacga aagctaatca tcacgcgctg gtaatgtcgt
     3301 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3361 tggcaaatcc gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac
     3421 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
     3481 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg
     3541 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3601 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3661 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3721 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3781 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3841 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc actcctgcga
     3901 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3961 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catactgtgc
     4021 gaaagcggtg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcgccaacg
     4081 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     4141 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4201 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4261 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4321 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4381 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4441 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4501 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4561 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4621 caggatcatc atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag
     4681 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4741 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4801 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4861 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4921 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4981 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     5041 ttccaggttg atggaacaac tgcctggtag ttactgataa taccgtcttt aataaccatc
     5101 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     5161 gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5221 gcactgtatt gattttgcag gatatcctgc aattcgcagc agtgaacggt acgaccaata
     5281 atacggccca gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga
     5341 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5401 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5461 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5521 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5581 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5641 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5701 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggca
     5761 ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa
     5821 gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag gttgatcggg
     5881 ttcgcgacac cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5941 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     6001 aggtttactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     6061 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     6121 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6181 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6241 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6301 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6361 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6421 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6481 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6541 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6601 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6661 caatagaaat caacagttct tccctggtcg ggaagtaggc gtaaccgccg cccgggttga
     6721 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6781 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6841 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6901 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgataaaa atcgccagca
     6961 acacactgat ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac
     7021 gcagacccgc ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     7081 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt
     7141 agcccgccga gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc
     7201 caagtgcaat gatgaagaac atcaccttgt tcagacgctt gagagaaacc ttccagccca
     7261 gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac
     7321 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7381 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7441 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7501 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa
     7561 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7621 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7681 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7741 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7801 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7861 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7921 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7981 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     8041 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     8101 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     8161 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8221 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8281 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8341 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8401 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8461 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8521 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8581 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8641 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8701 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8761 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8821 cgcctgcaga gacgttaccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8881 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8941 tacagccata gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt
     9001 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     9061 agccttcacg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     9121 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cgatttttgt
     9181 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9241 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9301 ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9361 aaccgatagc gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg
     9421 gctcaccagc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa
     9481 ccaacacata ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat
     9541 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt
     9601 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9661 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9721 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9781 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9841 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9901 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9961 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
    10021 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
    10081 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
    10141 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10201 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10261 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta
    10321 tatattatca tatatagcaa tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10381 catgcttgtt taagacgtaa tcccgtaaga gataaaccca tcaggagaga tatttatcga
    10441 accatttcag cgtcctttgc caggcaagat cggcggcaga tttgtcataa cgaggcgtgg
    10501 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcatag accttattat
    10561 tggctttcaa cgccgcctca taagcgggcc agccctcgtt gattcgggtg tccagttccg
    10621 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10681 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacac
    10741 caccgccata gcaaaatccg gtaataccca cttatgcctg tcgcttgcgg atagcgttgc
    10801 ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc
    10861 agctcacgac ctttatcatc atttcccgga taacctc
//
LOCUS       DADLTD010000147        10856 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLTD010000147 DADLTD010000000
VERSION     DADLTD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.147"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            83..424
                     /locus_tag="JN701_002995"
     CDS             83..424
                     /locus_tag="JN701_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY7365366.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="JN701_002996"
     CDS             complement(415..1341)
                     /locus_tag="JN701_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7365367.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="JN701_002997"
     CDS             1431..2429
                     /locus_tag="JN701_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY7365368.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="JN701_002998"
     CDS             complement(2426..2644)
                     /locus_tag="JN701_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365369.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2622..4661)
                     /gene="ligA"
                     /locus_tag="JN701_002999"
     CDS             complement(2622..4661)
                     /gene="ligA"
                     /locus_tag="JN701_002999"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY7365370.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGSGDGKRAAD"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JN701_003000"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JN701_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY7365371.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="JN701_003001"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="JN701_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY7365372.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="JN701_003002"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="JN701_003002"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY7365373.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="JN701_003003"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="JN701_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY7365374.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="JN701_003004"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="JN701_003004"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY7365375.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="JN701_003005"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="JN701_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY7365376.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
BASE COUNT         2647 a         2804 c         2687 g         2718 t
ORIGIN      
        1 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctggat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcac tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctggga attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctccgc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcacccg caatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccgatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc catgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat agacaggctg
     5341 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctagg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactctc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctattaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttacagctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 aaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttccta ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcaac tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg cagaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgccc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgccca
//
LOCUS       DADLTD010000148        10681 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLTD010000148 DADLTD010000000
VERSION     DADLTD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.148"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     regulatory      77..173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            208..996
                     /gene="thiM"
                     /locus_tag="JN701_003006"
     CDS             208..996
                     /gene="thiM"
                     /locus_tag="JN701_003006"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY7365377.1"
                     /translation="MQVDLQSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLPHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            993..1793
                     /gene="thiD"
                     /locus_tag="JN701_003007"
     CDS             993..1793
                     /gene="thiD"
                     /locus_tag="JN701_003007"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY7365378.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIESDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQYV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            1858..2676
                     /locus_tag="JN701_003008"
     CDS             1858..2676
                     /locus_tag="JN701_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 25 family protein"
                     /protein_id="HAY7365379.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQKVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            2728..3474
                     /locus_tag="JN701_003009"
     CDS             2728..3474
                     /locus_tag="JN701_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7365380.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(3448..4413)
                     /locus_tag="JN701_003010"
     CDS             complement(3448..4413)
                     /locus_tag="JN701_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7365381.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHTGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4410..5414)
                     /locus_tag="JN701_003011"
     CDS             complement(4410..5414)
                     /locus_tag="JN701_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAY7365382.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSITRHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(5411..6688)
                     /locus_tag="JN701_003012"
     CDS             complement(5411..6688)
                     /locus_tag="JN701_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365383.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPGTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFCESDNEITAIKVDDRDIALTQGEVK"
     gene            6945..7997
                     /gene="fbaB"
                     /locus_tag="JN701_003013"
     CDS             6945..7997
                     /gene="fbaB"
                     /locus_tag="JN701_003013"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY7365384.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            8305..9159
                     /gene="gatY"
                     /locus_tag="JN701_003014"
     CDS             8305..9159
                     /gene="gatY"
                     /locus_tag="JN701_003014"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY7365385.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            9188..10450
                     /gene="gatZ"
                     /locus_tag="JN701_003015"
     CDS             9188..10450
                     /gene="gatZ"
                     /locus_tag="JN701_003015"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY7365386.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            10460..>10681
                     /gene="gatA"
                     /locus_tag="JN701_003016"
     CDS             10460..>10681
                     /gene="gatA"
                     /locus_tag="JN701_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY7365387.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIY"
BASE COUNT         2700 a         2708 c         2790 g         2483 t
ORIGIN      
        1 tgagtctttg gcttcccgct ggcatgatcc agaacaggga ttaacgggac gttaatcgca
       61 atcttttgat tttgttttaa atgactcggg gtgcccttct ttgtgaaggc tgagaaatac
      121 ccgtattacc tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc
      181 ttcttcacgt tatggcagga gcaaactatg caagtcgacc tgcagagttc agcgcaatct
      241 gcgcacgcgt tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaacgat
      301 gtggtgcaaa cctttactgc caataccttg ctggcgctcg gtgcatcgcc agcgatggtt
      361 atcgaaaccg aagaggccag tcagtttgcg gctattgcta gtgcgttgct gattaacgtt
      421 ggcacgctga cgcagctacg cgcacagtcg atgtgtgccg ccgttgagca agcaaaaagc
      481 tctcaaacac cctggacgct tgatccagta gcggttggtg cactcgatta tcgccgccgt
      541 ttttgtctgg aacttctgcc tcataagcca accgctatac gtggtaatgc ttcggaaatc
      601 atggcattag ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct
      661 aacgcgatac ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact
      721 ggcgagatgg attatgttac cgatggacat cgtatcattg gtattcacgg tggcgatccg
      781 ttaatgacca aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt
      841 gcgttaccag gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc
      901 ggagaacgcg cagtcgccag aagcgagggg ccaggcagtt ttgttccaca tttccttgat
      961 gcgctctggc aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg
     1021 ccggtactga tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac
     1081 ttggcgctta tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac
     1141 agtcggtgta tcgcattgag tctgattttg tcgccgccca gctcgattcg gtgttcagcg
     1201 atgtgcgaat cgacaccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg
     1261 tggcagaacg gttgcaacgt tatcagatcc aatacgtggt actcgacacc gttatgctgg
     1321 caaaaagcgg cgacccgctg ctttcacctt cggcggttgc tacgctgcgc agtcgattat
     1381 tgccacaggt ttcattaata acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc
     1441 cacacgcgcg caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct
     1501 gtggcgcagt gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt
     1561 ttacccgcga gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg
     1621 gcactggttg tacgctctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg
     1681 ctgacaccgt acaggaggca aaaagctggc tttcatcagc attagcccag gccgacacgc
     1741 tggaagttgg tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt
     1801 actatagctg ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg
     1861 cagctaagaa ttaccagtcg aaaaaaactc acctctctat tatgcgctct tgggctaatt
     1921 tccatcgttg caatttatcc gcgtcagaag gtgaattttt tctattcgac agcagttcag
     1981 attacagact acatccattt ctacggttat cgcccggtta aatctttcgc tattcgcatt
     2041 cctgccagtt acaccattca cggaatagat gtttctcgct ggcaggagcg gatcgactgg
     2101 cagcgggtgg caaaaatgcg cgacaacggt atccgcttac agtttgcttt tattaaggcg
     2161 acggaaggcg aaaagctggt ggacccgtat ttttcgcgta actggcaact aagccgcgaa
     2221 aatggcctgc tgcgcggggc gtatcattat ttctccccgt cggtagcagc gccggttcag
     2281 gcacgattat ttctgcaaac ggtggatttc tcacaaggcg atttccctgc ggtactggac
     2341 gtagaagaac ggggaaaatt atcggcaaaa gaattacgca aacgagtaag tcagtggcta
     2401 aaaatggtcg aaaaaagtac gggaaaaaag ccgattattt actcaggagc cgttttttat
     2461 cacaccaatc tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctattatcaa
     2521 cgtcgtccgg acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag
     2581 gtagatggca tcaatggtcc ggtggatttt aatgtattta atggcacggt ggaggagttg
     2641 caggcattcg ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca
     2701 ccagactccc cggcaggaac aagacaaatg gaacaagcgc atacccagct gattgcccaa
     2761 ctgaacgagc gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg
     2821 gtaaaaaatg ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt
     2881 gatttaagtc agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg
     2941 gaagaagagg gtgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc
     3001 gaatactcgt tgaaagaagc gcgaggtttt tctcagcagg tggtattgcg cgggaaaaag
     3061 cccgatacgc tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag
     3121 ctggcggtcg aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa
     3181 gaagcggtat cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc
     3241 atcgggattt cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt
     3301 tcccgcgtta gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc
     3361 aaaataccgg tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag
     3421 tacagcatta gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc
     3481 gcgcgttggc gcacaatcac cgccccgatg cccgacaacc catgacgcca ctgcattgcc
     3541 cagtaatacg gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccaccggt
     3601 atgactgtcg cccgccccaa tggtgtcgac aacttgcgtt ggaaatgccg gaatgcagcc
     3661 agaagcgtcg ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc
     3721 ggcaaatttc tcctgccatt gcttgccaag tgttgttatc tctgcggata aagcaaaacg
     3781 ttcggcggca atctcagcct cttgacgatt gagtgacact aaaggtcgac aggccatgat
     3841 ccgcgccagt aatgcgtccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt
     3901 cacgtcttgc agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg
     3961 ataaccggaa aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg
     4021 attccactga ttttccacac cgctgaatga cataaaagta cgttcgccat ccggctcaac
     4081 cagcgccaga caccagccgt tatcgccttc ggcgttatcg atcaggctga ttaacccctc
     4141 tttcgccatc cggttgcgaa taatctccgc ccataccccc tgaccgagcg gcaaggcatt
     4201 acccgcttcg atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac
     4261 gttaacgctc tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc
     4321 ggcgatcacg tcaatgaccg ccgcgccgac gaccatcacc gactgacgcg tggttaattc
     4381 aggcagcagc gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc
     4441 gccagcgctg tggcatagcg gttgaaatca agctgattta ccgcatccag ctccgccttt
     4501 aacgcaggat cgatagcgtt aatgccatgc aacgcgccgc agattgccgt cgccatagca
     4561 ccgatggtgt ctgtatcgcc gccaaggtta gcgcagagga cggcgcaacg gttcgggtcg
     4621 gtttgtgcca gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct
     4681 gcgccaacga cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca
     4741 attttcagcg caatctccag ccgcgccgcc agtgaggcgc tgaacgtggt ggtgcgtttt
     4801 tgctgggcat gtcgcgtaat tgaaggcagt gaatcaacaa tcgctgacca gctttctccg
     4861 tcaatagcac gagaaatcgc ccatgcgatg actaccgcac ctgcaactgc cagatcggat
     4921 ttatgggtcg ggctcgacgc cagcgccaca tcatcaataa aggaatcaac atcgcgcgcc
     4981 ggaagcaaac aacctaatgg cgagacgcgc atcgctgcgc cgttggttac gccgttgttt
     5041 tccagttcag caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga
     5101 cctagcacgt ttttgttgaa ggcgtcgaaa cgcagcgccc agtcgaggat attacgccca
     5161 atcagatccg gatcgatctt gcctttacgt tccagcaacg catccgccag acacagcgcc
     5221 atcgaggtat cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt
     5281 ggtccaggca gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa
     5341 agctcggagg gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata
     5401 cgttctgttt tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga
     5461 tagccgtaat ttcgttgtcg gattcgcaga aaaaaatcat gaacagcacg gcgataatgg
     5521 caatcatcac cgcgccaaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg
     5581 gttcctgata agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac
     5641 tgccgaagcc ctggcagcag agcgtgatca gcccctgcgc ggcggtacgc atatgcacgg
     5701 gggctttttt atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt
     5761 gaagcaaaat accgaggaac agtaacgcgt aggtgaaata ttcatccgca ctaccgtaaa
     5821 taaagaagcc atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac
     5881 caaagcgttt agtgaaaaac ggtaatgcca gcataaagaa tatttcagag aactggccga
     5941 gcgtcatcca gccggtggca tttttcatgc cgacttcggt cagataaccg ttggcaaaga
     6001 tgtaatagaa cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt
     6061 ttttatcgcg cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc
     6121 cggtgctttt tggtggcgtg ccgggcagga aaaacgcaaa cacaccgagc agagcagaac
     6181 ttccggcggt aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt
     6241 gcggcaagaa accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa
     6301 tgcgcgggaa atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg
     6361 caatggtcgg catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag
     6421 tggtctgttg cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca
     6481 ctttttgcgc cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg
     6541 ccgcaatggc ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac
     6601 ttaaccacaa ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca
     6661 tgaacgacag ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg
     6721 tacgcgatga caataccact taataatacc ttttaaatac ctttacggtg aaaacttgac
     6781 cgcgctaaca ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa
     6841 gatggaaaaa ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga
     6901 caatccatag ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc
     6961 agttgcttgg caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg
     7021 accagcttta tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc
     7081 cgccagcggt gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag
     7141 gatatctttc tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg
     7201 ctgctaaccc gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct
     7261 gtaactgtgt ggtatcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc
     7321 gcattccatt tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg
     7381 atcaaacgct gtatgccagc gtggagcagg ctttcaacat gggcgcggtg gcggttggtg
     7441 cgactatcta ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggctt
     7501 ttgaacgtgc gcatgagctg ggcatggtga ccgtgctgtg ggcctatttg cgtaactccg
     7561 cctttaagaa agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc
     7621 atctggcggc aaccatcggc gcagatatcg tcaaacaaaa aatggcggaa aataacggcg
     7681 gctataaagc gattaattac ggttataccg acgatcgcgt gtacagcaag ttaaccagcg
     7741 aaaacccgat tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt
     7801 tgataaactc cggcggtgct gcaggcggtg aaactgacct cagtgatgca gtgcgtactg
     7861 cggttatcaa caaacgcgct ggcggaatgg ggctgattct tggacgtaag gcgttcaaga
     7921 aatcgatggc tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca
     7981 aaattactat tgcctgatac gccttatacg aacaacacca ccccgtagga cggataaggc
     8041 gtttacactg catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta
     8101 catagcactg ccacgtatgt ttacaccgca tccggcataa aaacacgcgc actttgctac
     8161 ggcttcccta tcgggaggcc gtttttttgc ctttcactcc tcgaataatt ttcatattgt
     8221 cgtttttgtg atcgttatct cgatatttaa aaacaaataa tttcattata ttttgaaaac
     8281 gaaaacaaac gacaggatat gaaaatgcac gtggtatcga caaagcagat gctgaacaac
     8341 gcacagcgcg gcggttatgc ggttccggca ttcaatattc acaatctcga aacgatgcaa
     8401 gtggtggtgg aaaccgctgc caacctgcat gcgccggtca tcatcgccgg aacgcctggc
     8461 acatttactc atgctggtac agaaaatctg ttggcgctgg tcagcgcgat ggcgaagcaa
     8521 tatcaccatc cactggcaat tcatctcgac catcacacga aatttgacga tatcgctcag
     8581 aaggttcgtt ctggcgtgcg ctcagtcatg attgacgcct cgcatttgcc ttttgcgcaa
     8641 aatatttcac gggtcaaaga ggtggtggat ttttgccatc gctttgatgt cagcgtcgaa
     8701 gcggagctgg ggcaacttgg cggccaggaa gatgatgtgc aagtcaatga agccgatgct
     8761 ttttatacca accccgctca ggcgcgtgaa tttgccgagg caaccggaat tgattccctg
     8821 gcggtcgcca tcggcacggc tcatgggatg tatgccagcg caccggcgct tgatttttct
     8881 agactggaga acattcgcca gtgggtgaac ttaccgctgg tgctgcatgg cgcgtcaggg
     8941 ttatcgacta aggatattca gcaaaccatc aaactgggga tatgcaaaat caacgttgca
     9001 acggagctga aaaatgcctt ctcgcaggcg ttaaaaaatt acctgaccga gcaccctgaa
     9061 gcgaccgagc cccgggatta tttgcagtcg gctaaatccg caatgcgcga tgtggtgagc
     9121 aaagtgattg ccgattgtgg ctgcgagggc agggcataac gcacctgcca tttaataagg
     9181 aaaaaacatg aaaacgttaa ttgcccggca taaagctggt gaacatatcg gcatatgttc
     9241 agtctgttct gcccatccgt tggttatcga agcggcgctg gcatttgatc gcaacagcac
     9301 gcgcaaagtg ctgattgaag caacgtcaaa ccaggtcaat caatttggcg gttataccgg
     9361 aatgacaccg gcagactttc gcgaatttgt ttttacgatt gccgataaag ttgggtttgc
     9421 acgcgaacgc attattctcg gcggcgatca tctggggcca aactgctggc agcaagaaaa
     9481 tgcggatgcg gcgatggaaa aatccgtcga gctggtaaag gaatatgttc gtgccggctt
     9541 cagtaaaatt catcttgatg cgtcaatgtc ctgcgcgggg gatcccatac cgttagcacc
     9601 agaaacggtt gcggaacgag ctgctgtgct ttgctttgct gcggaaagtg tggcgacaga
     9661 ttgccagcgt gagcaactga gctatgtcat tggcaccgaa gttccggttc cgggcggtga
     9721 ggccagcgcc attcagtcag tacacatcac ccatgttgaa gatgccgcca atactttacg
     9781 tacgcatcaa aaggccttta ttgtccgtgg gctgacagag gcgttaacac gcgtgattgc
     9841 catcgtggtg cagccgggcg tggaatttga tcacagcaat attatccatt atcagccgca
     9901 ggaagcgcag ccgctggcgc aatggataga aaacacccga atggtttatg aagcacattc
     9961 taccgattac cagacccgga cggcttattg ggaattagtc cgcgatcact ttgcaatatt
    10021 gaaagtcggt cccgcattaa cctttgcttt acgcgaggcg atatttgcac tggcacaaat
    10081 tgagcaggaa cttatcgccc ctgaaaatcg cagcggttgc ctggcggtaa ttgaagaagt
    10141 gatgctcgac gaaccgcaat actggaaaaa atattatcgt acgggtttta acgattcatt
    10201 actggatatt cgttacagcc tgtcggatcg tattcgttat tactggccgc atagtcggat
    10261 taaaaatagc gtcgaaacga tgatggtgaa tcttgaaggc gtggacatcc cactgggcat
    10321 gattagtcag tatcttccca aacaatttga acgcattcag tccggggaat tatcagcaat
    10381 accgcatcag ctgattatgg ataaaattta tgatgttttg cgcgcctatc gctacggctg
    10441 tgcggaataa ggacggtata tgactaacct gtttgttcgt agcggaattt cttttgtcga
    10501 tcgtagcgaa gttttaaccc atatcggtaa tgagatgctc gccaaaggtg tggttcatga
    10561 tacctggcca caggcattaa ttgccagaga agcagaattc cctaccggga taatgcttga
    10621 gcagcacgcc attgcaatac cgcattgtga ggcgattcat gctaagtcgt cagccattta
    10681 t
//
LOCUS       DADLTD010000149        10675 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.149, whole genome shotgun sequence.
ACCESSION   DADLTD010000149 DADLTD010000000
VERSION     DADLTD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.149"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..2119
                     /locus_tag="JN701_003017"
                     /pseudo
     CDS             1..2119
                     /locus_tag="JN701_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
     gene            2178..2381
                     /locus_tag="JN701_003018"
     CDS             2178..2381
                     /locus_tag="JN701_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY7365388.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            2561..3406
                     /locus_tag="JN701_003019"
                     /pseudo
     CDS             2561..3406
                     /locus_tag="JN701_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142672.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3434..3523)
                     /locus_tag="JN701_003020"
     tRNA            complement(3434..3523)
                     /locus_tag="JN701_003020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3487..3489),aa:Ser,seq:cga)
     gene            3617..4414
                     /gene="mtfA"
                     /locus_tag="JN701_003021"
     CDS             3617..4414
                     /gene="mtfA"
                     /locus_tag="JN701_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY7365389.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4515..4590
                     /locus_tag="JN701_003022"
     tRNA            4515..4590
                     /locus_tag="JN701_003022"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4548..4550,aa:Asn,seq:gtt)
     gene            4904..10675
                     /locus_tag="JN701_003023"
                     /pseudo
     CDS             4904..10675
                     /locus_tag="JN701_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
BASE COUNT         3066 a         2586 c         2610 g         2413 t
ORIGIN      
        1 accgctatga aaaaggcggg aaagatggca tcacaatgac atttatcggc ccttccccct
       61 ctgttcagga caaatcagcc agcactgata ataccaacat caacggcgaa gacatgactg
      121 agattgagga gagcatgctt ctacctgtct ccggtcagga actgcccatt cgttggcttg
      181 ctcaacacgg cagcgaaaaa ccagtaacgc acgtttcacg cgacgaactc caggcattac
      241 acattgcacg ggctgaagaa ctaccggctg ttactgccct ggctatttcg cataaaacca
      301 gtctgctcga ctcgctggag attcgcgacc tccacaaact ggttcgtgac actgacaaag
      361 ttttccctaa tcctggtaat tcagacctgg gactaataac tgcttttttc gaagcatact
      421 tggacgctga ctacactgat cggggcctgc tgacaaaaga gtggatgaaa ggaaatcgtg
      481 tttcacgcat cacccgcacg gcttccggtg ctaatgctgg tggtgggaac aaaaccgatc
      541 gcaatccgaa tttagtacac accctcgaca cactggatgt ggagattgca gcagccacac
      601 ttccgatgga ttttaatatt tatgaaattc cgggcagcgt ttatcgtcgc gcaaaagaag
      661 tagtcctgaa aaaagaaagt ccgttcaaag aatggtccgc agcacttcgt gcacccccgg
      721 gtattctgga ctattcccgc gccgctattt ttgcacttat ccgaagcgca caccctgaat
      781 tttatcacta cccgggacgc cttcaggggt atatcaacgc ctatttgacg gaaactgatc
      841 acgagaaccc cagcaaggaa actctcacag ctacccggca tacgccggaa aaagatatcc
      901 tggaagaaat taaccgcgag gtggttactg agcgtgaaac agaagaagaa aaaccacaac
      961 catctgacgc aatggcaggt gaacaggcaa caactgaaac aatggaaccg gatacaactg
     1021 aacattgcca gaacgcgcag tcgctggatg ctcagtcgca ggtgagttcc gctaaccaag
     1081 taaaagtcac cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg
     1141 acgccgataa gttacttgct gtatcgcgtg gtgaatttgt tgaggggatt agcgacccta
     1201 atgatccgaa atgggtcaag gggatccaga ctcgcgattc tgtgagccag aaacgaaaca
     1261 atccgaacca gtagcgcaac aggaaccgga aaaagtctgc accgcctgcg gtcagagcgg
     1321 tggcggcaac taccctgatt gtggcgcggt gatggacgac gcaacatacc agaaaacatt
     1381 cgatgaagag aatcaggttg aagttcagga aaatgatccg gaggaaatgg aaggcgctga
     1441 acatccacac aaggagaatg ctggcagcgc tcaggaccac gccagcgata gtaaaactgg
     1501 cgagacggca gatcccttaa ttacggtgaa tggtcatcac gaaatcacat ccaccagcag
     1561 gacgtgtgac catctaatga tcgaccttga aaccatggga aaaaatcctg atgccccgat
     1621 catctcaata ggtgcaatat ttttcgatcc gcaaaccgga gatatgggac ccgaatttag
     1681 taagactatc gatctggaaa ctgctggcgg agtcattgat cgggacacca ttaaatggtg
     1741 gcttaagcaa tcacgcgaag cgcaatctgc cattatgacc gatgaaatcc cgttagatga
     1801 tgcactgtta caattgcggg aatttatcga cgaaaactcc ggtgaatttt ttgttcaggt
     1861 ctggggaaat ggagccaact tcgacaacac gattttgcgc cgttcatacg aacggcaggg
     1921 gatcccctgc ccgtggcgtt actacaacga tcgcgatgta cgcacaatcg ttgagctggg
     1981 gaaagccata gacttcgatg ccagaacggc tattccattc gaaggtgagc gccataatgc
     2041 acttgatgac gctcgttacc aggcaaaata cgtttcagct atctggcaaa aactgatccc
     2101 gagtcaggct gatttttaat gttcaaccct aattgccgct aaccgtatat agttagcggc
     2161 ggttatgaga tatagctatg agcagcttat ttttaaccga agatgaattg ctaatattaa
     2221 cgggctgcaa atatgcaagc caccagcgaa aatggttaat ggaaaacgag cttccgttct
     2281 ataccaatcg tagtggcaaa ccgattgtca gccgggatct atttacctgc aataaaactt
     2341 taccaccacg cgaggtagag ccgaattttg gtgcgatctg atgggaagac gaaggaaaaa
     2401 tcctgagcac gaaaaattac ctccaaatgt atacccaaat aaatatagtt atgtatggaa
     2461 accaacatcc agcgaatctg taacactaac cgccatcaag gatggtttag ctgctttatg
     2521 gaaaaagtat gaggaaactg taaataatcg cgatcgtgca atgacattcg gtcgcttgtg
     2581 ggaaaaattc ctcgccagcg cctattacag tgacctcagt ccaagaacac aaaaagatta
     2641 tctgcaacat caaaaaagtt gcttgccgta ttcggtaagg tgccggcaga ttccataaaa
     2701 ccagaacaca tccgtcgata catggacaag agaggggaac agagtaaaac gcaagccaac
     2761 catgaagtcc cgcgtttaca gttgggggta tgagcgaggg tacgtgaagg ctaacccatg
     2821 tgcaggtgta agtaaattca aggccaaaaa ccgcgaacga tatgtaaccg acaaagaata
     2881 ccaggcagta ttaagcgttg cacctcttcc tgtttttatc gcaatggaaa ttgcctatct
     2941 gtgtgcagcg agggtttccg atgtgttatc gctgaaatgg gagcagattg gaaacgacgg
     3001 gatctttatc cagcaaggga aaacaggaaa aaaacagata aaagcatgga gtccacgatt
     3061 acaggcggcg atcgaaaaag caaaacagtt accaacatcc gcctatgtaa tcagcaatca
     3121 atacggcaac cgatatatgt acaaaggctt taacgaaatg tgggtagaag caagaaatcg
     3181 cgcaggcaaa atttcaggta ttttaaccga cttcaccttt catgatctga aggcgaaagg
     3241 aatttcagac tatgaaggaa gcagtcggga taagcaactt ttctctggtc acaaaaccga
     3301 ggggcaagtg ctaatctatg acaggaaggt taaagtttca ccgacacttg atgtcccgtt
     3361 acctgaaaat attccaagta attccacgtg tgatttttgt cactgactta atgatgtata
     3421 agtgattgaa ttttggcgga gagaggggga tttgaacccc cggtggagtt gcccccactc
     3481 cggttttcga gaccggtccg ttcagccgct ccggcatctc tccgttcaga tggttgccat
     3541 gatgccagga aatttggcat tttaacagtc cctgtccgtg caattttgtt caagtgacga
     3601 gtttgcgagc aaaacgatga ttaagtggcc ctggaaagta caagaatcag cacatcaaac
     3661 tgcccttccc tggcaggaag cactatcgat ccccctttta acgtgtctga cggaacagga
     3721 acaaagcaaa ttagtcactc ttgccgaacg ttttttacag caaaagcggc ttgttccttt
     3781 acagggcttt gaactggatt cattaagaag ctgccggata gcacttctat tttgcctacc
     3841 cgttctggag ttaggactgg aatggctgga tggttttcat gaagtcttaa tttatcctgc
     3901 gccatttgtg gtcgatgatg aatgggaaga cgatatcggt ctggtgcata accaacgtat
     3961 tgttcagtca ggtcagagct ggcagcaagg gcctatcgtt ttgaactggt tggatataca
     4021 agattctttt gatgcttctg gttttaacct gattattcat gaagtcgctc ataagctgga
     4081 cacccgtaac ggcgatcgcg ccagcggagt tccctttatt ccgttgcgtg aggttgctgg
     4141 ctgggaacac gatcttcatg ctgcaatgaa caacattcag gaagaaatcg aattggttgg
     4201 cgagaatgcg gcgagcattg atgcttatgc agccagcgat cctgctgaat gttttgccgt
     4261 actttctgaa tatttcttta gcgccccaga actttttgct cctcgtttcc cttcattgtg
     4321 gcaacgtttc tgccaatttt atcaacaaga tcctttgcag agactgcatc acgctaatga
     4381 tacagactcg ttttcggcga cgaatgttca ttaattaaca actttgcata ttaattaacc
     4441 aattgaaatg acttatgaaa tttagtgttg acagacaagg taccgctaag taatatgcgc
     4501 cccgttcaca cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
     4561 tcactggttc gagtccagtc agaggagcca aattctaaaa attcgctttt ttagcgcaat
     4621 gtcactgacc ttagttgaac attgtttttt aacggatagc gggtttttaa catcttaagc
     4681 gccctcgacc tttatggttg agggcgtttt gctatgaacg ccatcaccat tttcccctcg
     4741 attataaaac ttgagttatt cagtagtctc ccctcttgca actcacaccc aaaactgcct
     4801 aacgaaaagt tattaatttt caatcatatt gctatcagta tttacatttt ttcgctgtgc
     4861 tagaaagggc gcatttatgt tagctcgttc agggaaggta agcatggcta cgaagaagag
     4921 aagtggagaa gaaataaatg accgacaaat attatgcggg atgggaatta aactacgccg
     4981 cttaactgcg ggtatctgtc tgataactca acttgcgttc cctatggctg cggcagcaca
     5041 aggtgtggta aacgccgcaa cccaacaacc agttcctgca caaattgcca ttgcaaatgc
     5101 caatacggtg ccctacatcc ttggagcgct ggaatcggcc caaagcgttg ccgaacgttt
     5161 cggtatttcg gtggctgagt tacgcaaact caaccagttt cgtacgtttg ctcgaggttt
     5221 taataatgtc cgccagggtg atgaactgga tgtcccggca caagttagtg aaaaaaaatt
     5281 aaccccgccg ccgggtaata gcagtgacaa cctcgagcaa cagatagcca gtacttcaca
     5341 gcaaatcggg tctctgctcg ccgaagatat gaacagcgag caagcggcaa atatggcgcg
     5401 tggatgggcc tcttctcagg cttcaggcgc aatgacagac tggttaagcc gcttcggtac
     5461 cgcaagaatc acgctgggcg tggatgaaga ttttagcctg aagaactccc agttcgattt
     5521 tctccatccg tggtatgaaa cgcctgataa tctctttttc agtcagcata ctctccatcg
     5581 tactgacgag cgtacgcaga ttaacaacgg cttaggttgg cgtcatttca ctcccacatg
     5641 gatgtcaggc atcaacttct ttttcgacca cgatcttagc cgttaccact cccgcgccgg
     5701 cattggcgcg gagtactggc gcgactatct aaaattaagc agtaacggct atttgcgact
     5761 gaccaactgg cgcagcgcac ctgaactgga caacgattat gaagcacgcc cggccaatgg
     5821 ctgggatgta cgcgcagaaa gctggctacc cgcctggccg caccttggcg gtaaactggt
     5881 ctatgaacag tattatggcg atgaagtggc cctgttcgat aaagacgatc ggcaaagtaa
     5941 tcctcatgcc ataaccgctg gacttaacta tacccccttc ccgctgatga ccttcagcgc
     6001 ggagcaacgc cagggtaaac agggcgaaaa tgacacccgt tttgccgtcg attttacctg
     6061 gcaacctggc agcgcaatgc agaaacagct tgacccgaat gaagtcgctg cacggcgtag
     6121 ccttgcaggc agccgttatg atctggtgga tcgcaacaac aatatcgttc tggaatatcg
     6181 caaaaaagaa ctggttcgcc tgaccctgac agaccccgtg acagggaagt caggagaagt
     6241 gaaatcactg gtttcgtcgc tacaaaccaa atatgccctg aaaggctata acgtcgaagc
     6301 caccgcactg gaagctgccg gtggcaaagt ggtcacaacg ggtaaagata ttctggttac
     6361 cctgccggct taccggttca ccagtacgcc agaaaccgat aacacctggc cgattgaagt
     6421 caccgccgaa gatgtcaaag gcaatttgtc gaatcgtgaa cagagcatgg tggtcgttca
     6481 ggcacctacg ctaagccaga aagattcctc ggtatcgtta agtacccaaa cattgaacgc
     6541 ggattcccat tcaaccgcca cactgacttt tattgcgcat gatgcagcag gtaatcctgt
     6601 tgtcgggctg gtgctctcga cgcgtcacga aggtgttcag gacatcaccc tttctgactg
     6661 gaaagataat ggtgacggaa gctataccca gatcctgacc acaggagcga tgtctggcac
     6721 gctgacgctg atgccacagc tgaatggtgt ggatgcggct aaagcccccg ccgtggtgaa
     6781 tatcatttct gtttcgtcat cccgaactca ctcgtcaatt aagattgata aggaccgtta
     6841 tctctccggc aatcctatcg aggtgacggt agaactgaga gatgaaaatg acaaacctgt
     6901 taaggaacaa aaacagcaac tgaataacgc agtcagcatc gacaacgtga aaccaggagt
     6961 cactacagac tggaaagaaa ccgcagatgg cgtctataag gcgacctata ccgcctatac
     7021 caaaggcagt ggacttactg cgaagctatt aatgcaaaac tggaatgaag atttgcatac
     7081 cgctggattt atcatcgacg ccaacccgca gtcagcgaaa attgcgacat tatctgccag
     7141 caataatggt gtgctcgcca atgagaatgc agcaaacacc gtctcggtca atgtcgctga
     7201 tgaaggaagc aacccaatca atgatcatac cgtcacgttt gcggtattaa gcggatcggc
     7261 aacttccttc aacaatcaaa acaccgcaaa aacggatgtt aatggtctgg cgacttttga
     7321 tctgaaaagt agtaagcagg aagacaacac ggttgaagtc acccttgaaa atggcgtgaa
     7381 acaaacgtta atcgtcagtt ttgtcggcga ctcgagtacc gcgcaggttg atctgcagaa
     7441 gtcgaaaaat gaagtggttg ctgacggcaa tgacagcgcc acaatgaccg cgaccgtccg
     7501 ggatgcaaaa ggcaacctgc tcaatgacgt catggtcact ttcaatgtta attcagcaga
     7561 ggcgaaactg agccaaaccg aagtgaatag ccacgacggg atcgccacag ctacgctgac
     7621 cagtttgaaa aatggtgatt atagggttac ggcctctgtg agctctggtt cccaggctaa
     7681 tcaacaggtg aattttatcg gtgatcaaag tactgctgcc ctgaccctca gtgtgccttc
     7741 aggtgatatc accgtcacca acacagctcc gcaacatatg actgcaacct tgcaggataa
     7801 aaatggcaac ccactaaaag ataaagaaat caccttctct gtaccaaacg acgtcgcaag
     7861 tcggttctcg attagcaaca gcggaaaagg catgacggat agcaacgggg ttgcaatcgc
     7921 ctccctgacc ggcacgttag cgggcacgca tatgatcacg gctcgtctgg ctaacagcaa
     7981 tgtcagcgat acacagccaa tgacgtttgt ggcggataaa gacagagcgg ttgtcgttct
     8041 gcaaacatcg aaagcggaaa tcattgggaa tggcgtggat gagacaactc tgacagcaac
     8101 agtgaaagat ccttttgata acgtggttaa aaatctgtca gtagtcttcc gcacctcccc
     8161 cgcagacacg caactgagtc tgaaggcgct taatactaat gagaacggta ttgctgaagt
     8221 tacccttaag ggcacggttt tgggtgttta tacagccgaa gccatactgc ttaacggtaa
     8281 aagcgatacg aaaatcgtca atattgtgcc cgataccagc aacgcgcagg tcaccctgaa
     8341 catcccagct caacaggtgg tgacgaataa cagcgacagc gtgcagctga cggcgacggt
     8401 gaaagatccg tcgaatcatc cggtggcggg aataacggtg aacttcacca tgcctcagga
     8461 cgtggcggca aactttaccc ttgaaaataa cggtattgcc atcactcagg ccaatggcga
     8521 agcgcatgtc accctcaaag catgcatacg gttaccgcaa cgttgggtaa caataatgcc
     8581 agcgatgcgc aacctgtcac attcgtggcg gataaggaca gcgcagttgt cgttctgcaa
     8641 acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgactctaac agcaacagtg
     8701 aaagatcctt ttgataacgc agtaaaagat ctacaggtta ccttcagtac caagccggca
     8761 gatactcaac ttagtcagag cacaagcaat actaacgaca gtggtgttgc tgaagttacc
     8821 cttaagggca cggttttggg cgttcataca gtcgaagcca cacttcctaa cggcaacggc
     8881 tatacaacga cagtcaatat tgcgcccgat accagcaacg cgcaggttac gctgaacatc
     8941 cctgctcaac aggtggtgac gaataacagt gacagcgtgc agcttacggc gatggtgaaa
     9001 gatccgtcga atcatccggt ggcggggata acggtcaact tcaccatgcc acaggacgtt
     9061 gcggcaaact ttaccctcga aaataacggc attgccatca cccaagccaa tggggaagcg
     9121 catgtcacgc tgaaaggtaa aaaagcgggt acgcatacgg ttaccgcaac gctgggtaat
     9181 aacaatacca gtgattcgca gccggtaacg tttgtggcgg acaaaacctc ggctcaggtt
     9241 gtcctgcaga tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc aacgctaact
     9301 gcaacggtta aagatcagtt cgacaatggg gtgaataatc ttccggtaac attcagctca
     9361 gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc tggcatcgcg
     9421 caggccactc tcgcaggcgt tgcctttggt gagaagacgg ttactgcatc actggctaat
     9481 aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc ggcaaaaatt
     9541 atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa cagctccggc
     9601 agcgtcatca ccgccacagt cgttgataat aatggctttc cggtgaaagg tgtgactgtg
     9661 aacttcacca gcaacgcagc gacagccgaa atgacgaacg gcggtcaagc cgtgacgaac
     9721 gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga atcaggagcg
     9781 agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag cacatcaatt
     9841 aatgtcaacg ctgatgcgtc tacggcacat ctcaccttgc tacaggcact ttttgataca
     9901 gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta cggcaacggt
     9961 gtcccccagc aggaggtaac cctcagcgtt tcaccaagtg aaggcgtgac ccccagtaat
    10021 aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac cgctacaaaa
    10081 gccggggttt atcaattgac ggcaaccctc gaaaatggcg attcgatgca acaaacagtg
    10141 acctatgtgc cgaacgtcgc gaatgctgaa atcacgctgg cagcctcgaa ggatccggtg
    10201 attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac agagggcaat
    10261 gcgatagcca acactgaggt aacatttact ctgccggaag atgtgaaggc gaacttcacg
    10321 ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt cacgctgaaa
    10381 ggtacaaaag caggcgctca tactgttaca gcatcgatga ctggcggtaa gagtgagcag
    10441 ttggtggtga actttattgc ggatacgctc actgcgcagg ttaatcttaa cgttaccgag
    10501 gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt gactgatgga
    10561 aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga tgtgagcgca
    10621 agctttactc tcggacaagg cggttccgcc attactgata tcaacggcaa ggctg
//
LOCUS       DADLTD010000150        10661 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.150, whole genome shotgun sequence.
ACCESSION   DADLTD010000150 DADLTD010000000
VERSION     DADLTD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.150"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            23..817
                     /gene="ydiB"
                     /locus_tag="JN701_003024"
                     /pseudo
     CDS             23..817
                     /gene="ydiB"
                     /locus_tag="JN701_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992329.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            849..1607
                     /gene="aroD"
                     /locus_tag="JN701_003025"
     CDS             849..1607
                     /gene="aroD"
                     /locus_tag="JN701_003025"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY7365390.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            1753..3348
                     /locus_tag="JN701_003026"
     CDS             1753..3348
                     /locus_tag="JN701_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAY7365391.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            3362..4513
                     /locus_tag="JN701_003027"
     CDS             3362..4513
                     /locus_tag="JN701_003027"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7365392.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(4556..5467)
                     /locus_tag="JN701_003028"
     CDS             complement(4556..5467)
                     /locus_tag="JN701_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7365393.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            5781..6552
                     /locus_tag="JN701_003029"
                     /pseudo
     CDS             5781..6552
                     /locus_tag="JN701_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            6572..7510
                     /locus_tag="JN701_003030"
     CDS             6572..7510
                     /locus_tag="JN701_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY7365394.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            7552..8841
                     /locus_tag="JN701_003031"
     CDS             7552..8841
                     /locus_tag="JN701_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7365395.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIECGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            8838..9131
                     /locus_tag="JN701_003032"
     CDS             8838..9131
                     /locus_tag="JN701_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7365396.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            9134..>10661
                     /gene="fadK"
                     /locus_tag="JN701_003033"
     CDS             9134..>10661
                     /gene="fadK"
                     /locus_tag="JN701_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416216.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAY7365397.1"
                     /translation="MHPTGPHLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKIGAVSVPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     L"
BASE COUNT         2766 a         2566 c         2747 g         2582 t
ORIGIN      
        1 ccgccacagt ttatcgcctg aaatgcagaa taaagcctta gaaaaagcgg gattgccatt
       61 tacctatatg gcctttgaag tggataacga tagttttccc ggagcaattg aaggattaaa
      121 agctctaaaa atgcgcggaa ctggtgtatc aatgccgaac aaacaactgg cttgttaata
      181 tgttgatgaa ttaacgccag cagccaaact ggtgggtgcc atcaacacca tcgttaatga
      241 tgatggctac ctgcgcggct ataacaccga cggcacgggc catattcgcg ccattaaaga
      301 gagcggtttt gatatcaaag gcaaaacaat ggtgctgtta ggggccggag gtgcctcaac
      361 ggctattggc gcgcaggggg caattgaagg tttaaaagaa attaaactct ttaaccgtcg
      421 ggatgagttc ttcgataaag ccctcgcctt cgcgcagcgg gttaacgaaa acaccgattg
      481 tgtcgtcacg gttaccgatc tcgccgatca gcaagccttt gctgaagccc tggcttccgc
      541 cgacatttta accaatggca caaaagtggg tatgaaaccc cttgagaatg aatcattagt
      601 taatgatatc agtctgttac atccgggact tctggtcact gaatgcgtgt ataacccgca
      661 tatgacgaag ttattgcagc aggcgcaaca agctggttgc aaaacgattg atggatacgg
      721 catgttgttg tggcaagggg ctgaacagtt cacgttatgg actggcaaag atttccctct
      781 ggaatatgtt aaacaggtca tggggttcgg tgcctgacag gctgaccgcg tgcagaaagg
      841 gtaaaaaaat gaaaaccgta actgtaaaag atctcgtcat tggtacgggc gcacctaaaa
      901 tcatcgtctc gctgatggcg aaagatatcg cccgcgtgaa atccgaagct ctcgcctatc
      961 gtgaagcgga ctttgatatt ctggaatggc gtgtggacca ctttgccgac ctctccaatg
     1021 tggagtctgt catggcggcg gcaaaaattc tccgcgaaac catgccagaa aaaccgctgc
     1081 tgtttacctt ccgcagtgcc aaagaaggcg gcgagcaggc gatttccacc gaggcttata
     1141 ttgctctcaa tcgtgcagcc atcgacagcg gcctggttga tatgatagat ctggagttat
     1201 ttaccggcga tgatcaggtt aaagaaaccg tcgcctgcgc ccacgcgcat gatgtgaaag
     1261 ttgtcatgtc caaccatgac ttccataaaa cgccggaagc cgaagaaatc attgcccgtc
     1321 tgcgcaaaat gcagtccttc gacgccgata ttcctaagat tgcgctgatg ccgcaaagta
     1381 ccagcgatgt gctgacgttg cttgccgcga ccctggagat gcaggagcag tatgccgatc
     1441 gtccaatcat cacaatgtca atggcaaaaa ctggcgtaat ttctcgtctg gctggtgaag
     1501 tatttggctc ggcggcaact tttggtgcgg taaaaaaagc ctctgcgcca gggcaaatct
     1561 cggtaaatga tttacgcaca gtattaacta ttttacatca ggcataagca ataatatttc
     1621 ggcgggaata tcctcccccg ccgaactaaa aaaaatatat tcaaccgtat ttaataaaaa
     1681 tatttcgtga gtctctgtgc gctaaatatc catctggcgt agggaaaatc acacctgaat
     1741 cagaaattaa caatgaaacc tgtaaaacca cctcgtatta atggacgagt gccggtcctg
     1801 tcggcacagg aagcggtgaa ttatattccc gacgaagcaa cactttgtgt gttaggcgct
     1861 ggcggcggta ttctggaagc cacgacgtta attactgctc ttgctgataa atataaacag
     1921 actcaaacgc cacgtaattt atcgattatt agtccaacag ggcttggcga tcgcgccgac
     1981 cgtggtatta gtccgctggc gcaagaaggt ctggtgaaat gggcattatg tggtcactgg
     2041 ggacaatcgc cgcgtatttc tgaactcgca gaacaaaata aaattattgc ttataactat
     2101 ccacaaggtg tacttacaca aaccttacgc gccgccgcag cccaccagcc tggtattatt
     2161 agtgatattg gcatcgggac atttgtcgat ccacgccagc aaggcggcaa actgaatgaa
     2221 gtcacgaaag aagacctgat taaactggtc gagtttgata acaaagaata tctctattac
     2281 aaagcgattg cgccagatat cgccttcatt cgcgctacca cctgcgacag tgaaggctac
     2341 gccacttttg aagatgaggt gatgtatctc gacgcattgg ttattgccca ggcggtgcac
     2401 aataacggcg gtattgtgat gatgcaggtg cagaaaatgg ttaagaaagc cacgctgcat
     2461 cctaaatctg tccgtattcc gggttatctg gtggatattg tggtggtcga tccggatcaa
     2521 agccaactgt atggcggtgc gccggttaac cgctttattt ctggtgactt cacccttgat
     2581 gacagcacca aacttagcct gcccctaaac caacgtaaat tagttgcgag gcgtgcatta
     2641 ttcgaaatgc gtaaaggcgc ggtggggaat gtcggcgtcg gtattgctga cggtattggc
     2701 ctggtcgccc gggaagaagg ttgtgctgat gactttattc tgacggtaga aacaggtccg
     2761 attggcggaa ttacttcaca ggggatcgcc tttggcgcga acgtgaatac ccgtgccatt
     2821 ctggatatga cgtcccagtt taatttttat cacggtggtg gtctggatgt ttgttatttg
     2881 agttttgctg aagtcgacca gcacggtaac gtcggcgtgc ataaattcaa tggcaaaatc
     2941 atgggtaccg gtggatttat tgatatcagt gccacctcga agaaaattat tttctgcggc
     3001 acgttaaccg cgggcagttt aaaaacagaa attgccgatg gcaaattaaa tatcgtccag
     3061 gaaggacggg tgaagaaatt tattcaggaa ctaccggaaa ttacttttag cggaaaaatc
     3121 gcgctcgaac gggaactgga tgttcgttac atcaccgaac gtgctgtatt cacgctgaaa
     3181 gaagacggcc tgcatttaat cgaaatcgcc cctggcgtcg atttacaaaa agatattctc
     3241 gacaaaatgg atttcacccc agtgatttcg ccagaactca aactgatgga cgaaagatta
     3301 tttatcgatg cggcgatggg ttttgtcctg cctgaagcgg ctcattaaca ggagtatcac
     3361 gatggatttt tctttaactg aagaacaaga actgctgctg gccagtattc gcgaactgat
     3421 tacgactaac tttgcggaag agtatttccg cacctgcgat caaaacggga catatccgcg
     3481 tgagtttatg cgggcgctgg cggataacgg catttctatg cttggcgtgc cggaagagtt
     3541 cggtggcatc cctgcggatt acgtcaccca gatgctggcg ctgatggaag tgtcaaaatg
     3601 cggtgctccg gcatttttga ttaccaacgg tcaatgtatt cacagtatgc gccgtttcgg
     3661 ttctgcagag cagctacgta aaacggcaga gagcaccctg gaaacgggtg atcccgccta
     3721 tgccctggcg ttgacggagc caggcgcagg ctcagacaac aacagtgcca ctaccactta
     3781 cacgcgtaaa aacggcaagg tttacatcaa cggacagaaa acctttatta ccggtgcgaa
     3841 agagtacccg tatatgctgg tgttggcgcg cgatccgcaa ccgaaagatc ccaaaaaagc
     3901 cttcaccctg tggtgggtcg actccagtaa gcccggcatt aagattaacc cgctgcataa
     3961 aatcggctgg catatgctca gcacctgcga agtctatctc gacaacgtgg aggttgaaga
     4021 gagcgacatg gtgggcgaag aaggaatggg tttcctcaat gtgatgtaca actttgagat
     4081 ggagcgcctg atcaacgccg cgcgcagcac cggctttgcc gaatgcgcct ttgaagatgc
     4141 cgcccgctat gccaaccaac gtatcgcttt tggtaagccc attggtcata accagatgat
     4201 ccaggaaaaa ctggcgctga tggcgattaa gatcgacaac atgcgcaaca tggtgctgaa
     4261 agtggcatgg caagccgatc agcatcagtc actgcgcacc agcgcggcgc tggcaaaact
     4321 gtattgcgca cgtaccgcaa tggaagtcat tgatgatgcg attcaaatca tgggcggtct
     4381 gggctatacc gatgaagcgc gcgtctcccg cttctggcgt gatgtccgtt gtgaacgtat
     4441 cggcggcggt acagacgaaa ttatgattta cgtagcaggt cggcagatcc tgaaagacta
     4501 tcagaacaaa taatctgcag gcggcgcagc ttcttaacaa actgcgccgc cagatttatc
     4561 caacaagact taccggttga ggaaattcag ataggatttc ttgttccgca aaacagtttt
     4621 taaattgtct acggtaatcg ctgggcgaac aaccgacatg gcgcaaaaac agtttggcaa
     4681 agtgatcgac attttcataa cccacacgcc aggagatctc tgcctgtgat aattcagtat
     4741 tagtgagtga ccattttgct tccgtcatac gccgttgaat aacatagtta ataggggaaa
     4801 tacgatactc tttggtaaat tcatggcaaa tataactgac gctggcacga aattttttcg
     4861 ataactgttc taaagtgatt ttttcgcgat aattattact cagatagaaa agaatatctt
     4921 ttatcagaac atctttttta atatacccct gctccgaacg ataagcattt ttaaaatttt
     4981 cgtagtaaag aatggctaac gtataggcaa atgcatccca taacgaagat gtttggctat
     5041 ttttactttg cggcaaaatc acacttagct cattaaataa ggtttttatg acttcttttc
     5101 cctgccccgc ggcaattacc ggacaagaat gcggttgcag tagctgattt tcctcagccc
     5161 cctgaaactg aaagccgtac agcgcacagg tacacgtcgt tgccggatcg ttaacgtcag
     5221 aggccaccgc atgcagcctg ccgcgttcta tcaccacaat gtcatccgca tgtgcgacat
     5281 ataacgaaga atcgatggta aaccttgcaa cccctttctt gacgtaaatc aactcggttt
     5341 cattgtcatg aacgtgatgt ccggactccc attttggatc atcgctaaat gcaaaacgtg
     5401 aaagccgtgg cgttttaccg gcgacaaaca gcgtttcact ggcgttatca aaacagcgtt
     5461 gatacatgac aacctcccta ttccatgagc aagcaaaaac aatatatgcc ggataaaata
     5521 cggcgtgtat tgcggttata caaccgcgtt tagcgagagc taaacaagat tatttacact
     5581 gaatgcaaga ttgtacggtc atggaataac tatattgccg caaattgcac ttttgtttta
     5641 gcgatcgcat ttttttgcaa gattgttggc aaggaaaaca gcttgctccg tcgaaaaccc
     5701 ccaccgctat cgcacactat tttcaggcca tttttacctt ccatcggaga tggttccgta
     5761 tgcgactcac aggagaaatc atgaaaataa taacctgctt taagctggtg cctgaagaac
     5821 aggacattgt tgtcactcca gaatacaccc tgaatttcga caatgccgac gccaaaatca
     5881 gccagttcga tctcaatgcc attgaagctg caagccagct cgcaacagat gacgatgaga
     5941 tagccgcgct gaccgttggc ggctcattgt tgcagaactc gaaagtgcgc agtgcgcaaa
     6001 gatgtgctat cccgcgggcc gcacagcctg tatttggtgc aggatgcgca acttgaacat
     6061 gcactgcctc tcgataccgc aaaagcgctg gcggcagcaa ttgaaaagat cggcttcgat
     6121 ttactgatct ttggtgaagg ttccggcgac ctttatgccc agcaggttgg cttgctggtc
     6181 ggagaaattc tgcaacttcc ggtgattaat gcagtgagtg ctattcagcg tcagggtaat
     6241 acactggtga ttgaacgcac gcttgaagat gatgttgaag ttattgaact ctctgttcca
     6301 gccgtgctct gcgtcacctc cgatattaac gtgccacgca ttccttcgat gaaagccatt
     6361 ctcggcgcgg gtaaaaaacc ggtaaatcag tggcaggcaa gtgatattga ctggagccag
     6421 agcgcgccac ttgctgaact ggtcggcatt cgcgtaccgc cgcaaacaga acgtaagcac
     6481 atcattatcg ataacgattc gccggaggcc attgccgagc tggcggaaca tctgaagaaa
     6541 gccctgaact gaagcctacg gagaagaaac gatgagtcaa ttaaacagcg tctgggtctt
     6601 tagcgataat cctgaacgtt atgctgaact gtttggcggc gctcagcaat ggggccaaca
     6661 ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca gttatgcctt atggtccaaa
     6721 atgtatttat gttcttgagc aaaacgacgc gctgcaacgc actgaaaatt acgccgaaag
     6781 cattgctgcc ccgctgaaag ataaacaccc cgctatgttg ctgttggccg cgacgaaacg
     6841 tggtaaagct ctggcagcac ggttaagtgt gcaactgaat gcggcgctgg tgaacgatgc
     6901 cacggcggtg gatattgtcg atggtcacat ttgcgccgaa caccggatgt atggcgggtt
     6961 agcgttcgct caggaaaaga tcaacagccc gctggcgatc attacccttg cacccggtgt
     7021 tcaggaaccg tgcaccagtg atacctctca tcagtgcccg acagaaacgg taccttatgt
     7081 tgctccgcgt catgaaattc tctgtcgcga acgccgtgcg aaagccgcaa gcagcgtgga
     7141 cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt ctggcggcgc aggatgacct
     7201 gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa gtcggctgtt cacgtccaat
     7261 tgccgaaggc gagaactgga tggagcgtga acgttatatc ggtgtctccg gcgtgttgct
     7321 gaaatccgat ctctacctga cgctggggat ctccgggcag atccagcata tggttggcgg
     7381 caacggcgca aaagtgattg tcgccatcaa taaagataaa aatgcgccaa tcttcaatta
     7441 tgccgactac ggtctggtgg gcgatatcta caaagtcgtc cctgccctga ttagccagtt
     7501 gagccgctaa tcatctcccg ctgtaaacgc agcgggaagg gagcataacg catgtcggat
     7561 gacaaatttg atgccattgt ggtcggtgcg gacgttgcta gtagcgttgc cgcactggtc
     7621 atggcgcgag ccggactgga tgtcctggtg atagaatgcg gcgacagtgc cggatgtaaa
     7681 aacatgaccg gcgggcgtct ttatgcccac acacttgaag caatcattcc aggctttgca
     7741 gtatcagcgc cggtagaacg caaggtcaca cgcgagaaaa tctccttctt aaccgaagaa
     7801 agcgccgtta ccctcgattt tcaccgcgag caaccagatg ttccgcaaca cgcatcttat
     7861 accgtattgc gtaatcgtct ggacccgtgg ttgatggaac aagccgagca ggcgggcgca
     7921 cagtttatcc cgggcgttcg cgtcgatgcg ttggttcgtg aaggaaacaa ggtcactggc
     7981 gtgcaggccg gggatgatat tctccaagcg aatgtggtgg ttctagctga tggcgtaaac
     8041 tcgatgcttg gccgctcgct gggaatggtt cccgcttccg atccgcatca ttacgctgtt
     8101 ggtgttaaag aggttattgg cctcacacca gaacagatca acgatcgctt taatattacg
     8161 ggcgaggaag gtgccgcctg gctgtttgcc ggctcccctt ctgacggcct gatgggcggg
     8221 ggatttctct ataccaacaa ggattccata tccttggggc tggtttgtgg attgggtgat
     8281 atcgcccatg cgcaaaaaag cgtgccgcaa atgctggaag attttaaaca acaccccgcc
     8341 attcgcccac taattagcgg cggcaaactg cttgaatatt ccgcgcatat ggtgccagaa
     8401 ggcggtctgg caatggtgcc gcagatggtt aacgatggcg tgatgatcgt tggtgacgcc
     8461 gcaggcttct gcctgaattt gggttttaca gttcgtggca tggatttagc cattgcatcg
     8521 gctcaggctg ccgccacaac ggtgatcgcc gccaaagaac gcgaggattt ctccgccagc
     8581 agtctggcgc aatacaaacg tgagctggaa caaagctgcg tcatgcgcga tatgcagcat
     8641 ttccgcaaga tcccggcgct gatggaaaac ccgcgcctgt ttagccaata ctcacgaatg
     8701 gtcgccgaca tcatgaacga gatgttcacc attgacggca aaccaaacca gccggtacgc
     8761 aaaatgatca tgggacacgc gaagaaaatt gggctgatca acttgctgaa agatggcatt
     8821 aagggagcaa ccgcgctatg agccagaacg ctacggttaa cgtcgacatc aaattaggcg
     8881 tcaataaatt ccatgttgat gagggccacc cgcatatcat tctggcggca aatcccgata
     8941 tcaatgaatt ccgtaaatta atgaaagctt gccctgccgg actttataag caggatgacg
     9001 ccggcaacat tcatttcgat tccgccggtt gtctggagtg cggcacctgt cgggtgctgt
     9061 gcggcaacac tattctcgaa cagtggcaat atcccgcagg caccttcggt attgattttc
     9121 gctacggcta atcatgcatc ccacaggccc gcatctcggg cctgatgttc tgtttcgaga
     9181 gtccaaaatg aaagtgacat taacgttcaa cgaacaacgt cgtgcggcgt atcgtcagca
     9241 agggttatgg ggcgatgctt cactggccga ttactggcag cagaccgctc gtgcgatgcc
     9301 agacaaaatt gccgtggtcg acaatcatgg tgcaacgtac acctatagcg cgctcgatca
     9361 cgccgcgagc tgtctggcaa actggatgtt agcgaagggt attgaatcag gcgatcgcat
     9421 cgcatttcaa ctgcctggct ggtgtgaatt taccgttatc tatcttgcct gcctgaaaat
     9481 cggtgcggtt tccgtgccgc tgttgccttc ctggcgggaa gcagaactgg tgtcggtgct
     9541 caataagtgt caggcaaaaa tgttctttgc accgacgttg tttaaacaaa cacgtccggt
     9601 agatttaatc ctgccgctgc aaaatcagct tccacaacta caacaaattg tcggcgtgga
     9661 caaactggct cccgccacct cttccctctc attaagtccg attatcgccg acaatacctc
     9721 actgaccacg gcgataacga cccacggcga tgaattagct gcggtgctgt ttacctccgg
     9781 aaccgagggt ctgccaaagg gcgtgatgct aacgcataac aatattctcg ccagtgagcg
     9841 ggcttattgc gcgcgactga atctgacctg gcaggatgtc tttatgatgc ctgcgccact
     9901 tggtcacgca acgggctttc tgcatggcgt aacggcacca ttcttaattg gcgctcgcag
     9961 cgtgttgtta gatattttca ctcctgatgc gtgtctcgcg ctgcttgagc agcagcgttg
    10021 cacctgtatg ctaggcgcaa cgccgtttgt ctatgatctt ttgaatgtac tagagaaaca
    10081 acccgcggac ctttcagcgc tgcgtttctt tctttgcggc ggtaccacaa tccccaaaaa
    10141 agtggcacgt gaatgccagc agcgcggcat taaattatta agtgtttatg gttccacaga
    10201 aagttcgccg catgcggtgg tgaatctcga tgatcctttg tcgcgcttta tgcacaccga
    10261 tggttacgct gccgcaggtg tagagattaa agtggtcgat gacgcacgca agaccttacc
    10321 gccaggttgc gaaggtgaag aagcctcgcg tggccccaat gtgtttatgg ggtattttga
    10381 tgaacctgaa ttaaccgccc gtgcgctgga cgaagaaggc tggtattgca gcggcgatct
    10441 ctgccgtatg gatgaggctg gctatataaa aattaccgga cgcaaaaaag atattattgt
    10501 ccgcggcggc gaaaatatta gcagccgtga agtggaagat attttattgc agcatcctaa
    10561 aattcacgat gcctgtgtgg ttgcaatgcc cgatgaacgt ttaggtgaac gatcatgcgc
    10621 ttatgtcgtg ctgaaagcgc cgcatcattc attatcgctg g
//
LOCUS       DADLTD010000151        10559 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLTD010000151 DADLTD010000000
VERSION     DADLTD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.151"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(72..1100)
                     /locus_tag="JN701_003034"
     CDS             complement(72..1100)
                     /locus_tag="JN701_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY7365398.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1275..2867
                     /locus_tag="JN701_003035"
     CDS             1275..2867
                     /locus_tag="JN701_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY7365399.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2877..4049
                     /gene="malY"
                     /locus_tag="JN701_003036"
     CDS             2877..4049
                     /gene="malY"
                     /locus_tag="JN701_003036"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY7365400.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="JN701_003037"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="JN701_003037"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY7365401.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5188..6228)
                     /locus_tag="JN701_003038"
     CDS             complement(5188..6228)
                     /locus_tag="JN701_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7365402.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6441..6566
                     /gene="blr"
                     /locus_tag="JN701_003039"
     CDS             6441..6566
                     /gene="blr"
                     /locus_tag="JN701_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY7365403.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6839..7054
                     /gene="ydgT"
                     /locus_tag="JN701_003040"
     CDS             6839..7054
                     /gene="ydgT"
                     /locus_tag="JN701_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY7365404.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7140..7580
                     /locus_tag="JN701_003041"
     CDS             7140..7580
                     /locus_tag="JN701_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY7365405.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            7657..8238
                     /gene="rsxA"
                     /locus_tag="JN701_003042"
     CDS             7657..8238
                     /gene="rsxA"
                     /locus_tag="JN701_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY7365406.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8238..8816
                     /gene="rsxB"
                     /locus_tag="JN701_003043"
     CDS             8238..8816
                     /gene="rsxB"
                     /locus_tag="JN701_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY7365407.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8809..>10559
                     /gene="rsxC"
                     /locus_tag="JN701_003044"
     CDS             8809..>10559
                     /gene="rsxC"
                     /locus_tag="JN701_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7365408.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQV"
BASE COUNT         2420 a         2734 c         2681 g         2724 t
ORIGIN      
        1 cctgtgtgcc acatttttta tcattaacct tgaggaaagg acactaccag gtatagcaga
       61 taaagagagg attatttcgc tgcaatgagc cgggcgggaa taataagatt gcgtgaatgc
      121 gtctcttcat gggtgatttt ttgcatcatg cgatccgcaa gtgtggtacc aagttcccgc
      181 gcaggcgtgc tggcccaggt aacgggtata tcatcaagtg tggttggtgt cgcatcggta
      241 aatgccgcca gcgaaacttg ttgctcaaag taacgatcga ctccgctttc cccgctttgc
      301 cgccctgctt tcagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt atagcaaacc
      361 acagcactga tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc agcttgcttc
      421 tggctggatg tgcactccag cacccaatcg ctgtgaaacg gcagaccaaa ttttagtaga
      481 gttgcacaat agcctcccac ccgttctgcc cgggttaatg aggaactttg ccctcccagc
      541 caggcgatcc gctgatgccc attgcgaatg agatgctccg tcaacaactg tgcagcctgc
      601 atgttgtccg ggcgaaccgt atcaacatca tcaagataac tggcacggga agcgaaaatc
      661 accgggatag ctttttcttc tgccatccgt cgcaggtcat cgctgcttcc tgcagccccg
      721 gcaattacca caccatcgac cccctgattc agtaacagtg aaaaccgctg tgccagctgc
      781 tcgccgtctt taccgccgtg aagcaaaaaa accatccgtc cctgcgcttc cagagcttcc
      841 gtcaatccgg ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac gatcaaacca
      901 atgacgccgc tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac aaatcccagc
      961 tcttcaatgg cggcgttaac gcgttctcct gtggcggtag agattcgccc tttgccacta
     1021 agcaccagcg aaacggtact taccgacacg cccgcagcca gcgcaacatc atgaatggtt
     1081 atttttttgg cggtagccat gcagatgacc tactccctga ttatgtgaca aataaaacgt
     1141 tttacctttt atttatctta tacccgctat tatcgttgcg taatgtgatt tatgcctcac
     1201 taaaatttga taaaacgttt tatcttctcg cgcaatttac tgaatccaga ttgttctcta
     1261 cgaggagtcg ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg gagttcttcc
     1321 agcagttagg caaaaccttc atgttacccg tggcattatt gtcgttctgc ggcattatgc
     1381 tcggcattgg tagttctctt agcagccatg atgtcataac cctgatcccg gtcctgggca
     1441 accccgtgtt gcaggctatc tttacctgga tgagtaagat tggctcgttt gcttttagtt
     1501 tcctgcctgt catgttctgt atcgccatcc cgctgggcct ggcacgcgaa aataaaggcg
     1561 tagcggcatt cgctggcttc gtcggttatg cggtaatgaa cctcgcggta aacttctggt
     1621 tgaccaataa aggcattctg ccaaccacgg atgccgcggt tctgaaagcc aataacatcc
     1681 agagcattct tgggatccag tcgatcgata ccgggatcct cggtgcggtg atcgccggta
     1741 ttatcgtctg gatgctgcat gagcgtttcc ataatatccg cctgccggat gcgctggcat
     1801 tcttcggcgg tacgcgcttc gtaccaatta tctcctcgct ggtgatgggc cttgtcggcc
     1861 tggtgattcc attagtctgg ccgattttcg ccatgggtat tagcggcttg ggccatatga
     1921 taaacagcgc gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt ctgctgttgc
     1981 cgtttggtct gcatcacatt ctggtggcat taattcgctt taccgacgca ggcggcacgc
     2041 aggaagtctg cggtcaaacc gtcagcggcg cactgaccat cttccagacg caattgagtt
     2101 gcccgaccac tcacggtttt tctgaaagcg ccacgcgttt cctttcgcaa ggtaaaatgc
     2161 ctgcgtttct cggcggtctg ccagctgcag cgttagctat gtatcactgc gcgcgcccgg
     2221 aaaatcgcca taaaattaaa ggtctgctga tttctggcct gatcgcctgc gtcgttggcg
     2281 gcactaccga accgctggaa ttcctgttcc tgttcgtagc gccagttctg tatgtcatcc
     2341 acgcgctgtt aaccggcctc ggcttcaccg tcatgtctgt gctcggcgtc accatcggta
     2401 ataccgacgg caatatcatc gacttcgtgg tgttcggtat tttgcatggt ctgtcaacca
     2461 agtggtacat ggtgccagtg gtggcggcaa tctggtttgt cgtttactac gccatcttcc
     2521 gtttcgctat cacccgcttc aatctcaaaa ccccggggcg cgatagcgaa gttgccagct
     2581 caatcgaaaa agccgttgcc gctgcgccgg gtaaatcagg ttacaacgtt cctgcaatcc
     2641 tcgaagcatt aggcggtgcc gacaatattg tcagcctcga taactgcatt acccgtctgc
     2701 gtttgtctgt gaaagatatg tcgcttgtta atgtgcaggc actgaaggac aatcgggcaa
     2761 ttggcgtagt acaacttaat caacataacc tgcaggttgt tatcgggcca caagttcagt
     2821 cagtaaaaga tgaaatggcc ggtctgatgc atactgtcca ggcataagga taagatatgt
     2881 tcgatttttc aaaggtcgtg gatcgtcatg gcacatggtg tacacagtgg gattatgtcg
     2941 ctgaccgttt cggcactgct gacctgttac cgttcacgat ttcagacatg gattttgcca
     3001 ctgccccctg cattatagag gcgctgaatc agcgcctgat gcacggcgta tttggctaca
     3061 gccgctggaa aaacgatgag tttctcgctg ctattgccca ctggttttcc acccagcatt
     3121 acaccgccat cgattctcag acggtggtgt atggcccttc tgtcatctat atggtttcag
     3181 aactgattcg tcagtggtct gaaacaggtg aaggcgtggt gatccacaca cccgcctatg
     3241 acgcatttta caaggccatt gaaggtaacc agcgcacagt aatgcccgtt gctttagaga
     3301 agcaggctga tggttggttt tgcgatatgg gcaagttgga agccgtgttg gcgaaaccag
     3361 aatgtaaaat tatgctcctg tgtagcccac agaatcctac cgggaaagtg tggacgtgcg
     3421 atgagctgga gatcatggct gacctgtgcg agcgtcatgg tgtgcgggtt atttccgatg
     3481 aaatccatat ggatatggtt tggggcgagc agccgcatat tccctggagt aatgtggctc
     3541 gcggagactg ggcgttgcta acgtcgggct cgaaaagttt caatattccc gccctgaccg
     3601 gtgcttacgg gattatagaa aatagcagta gccgcgatgc ctatttatcg gcactgaaag
     3661 gccgtgatgg gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc
     3721 agcaaggcgc gccgtggctg gatgccttac gcatctatct gaaagataac ctgacgtata
     3781 tcgcagataa aatgaacgcc gcgtttcctg aactcaactg gcagatccca caatccactt
     3841 atctggcatg gcttgattta cgtccgttga atattgacga caacgcgttg caaaaagcac
     3901 ttatcgaaca agaaaaagtc gcgatcatgc cggggtatac ctacggtgaa gaaggtcgtg
     3961 gttttgtccg tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat
     4021 taattaacgc catccgcgct gttcgttaac cccaattgcg caacgtaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacttt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcccttg atttagccgg tgatgaactt ggtttcccgg
     4681 gaagtctgtt cctttctcac ttcaaccttg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc ggcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgcgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc tgaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaatg
     5161 gatggtgcaa attgcaccat ccatttttca tgcaaggcac aaagtcgcgc gatgtttggc
     5221 ggattcgatg cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg
     5281 attttcacca tcgccattta acgcatcacg aatagccgca tagtaagccg gataattccc
     5341 aggtaccgtc aacagcgttt cttcgacacg ttcctcacct tccactcggg taagtacgcc
     5401 atcacgcata tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc
     5461 ttcctgtgga tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg
     5521 tgctgactca gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga
     5581 caagatggcg tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac
     5641 cgtcatgctg accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa
     5701 atcgtaccag ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg
     5761 acggaagcgg tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag
     5821 caaacctttt agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac
     5881 acgccccagg ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa
     5941 gggtttatca acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg
     6001 ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt
     6061 cggctcagag acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct
     6121 ggagattacc gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt
     6181 gctcgcataa ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct
     6241 cattgaagta tgattgctat ttgacactat cctttaccca cgctcaacag tttaatatta
     6301 agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa tatcgcaaaa acctcagtaa
     6361 aaatcttgct ggagctatta ttgctaagta acatttaccc cctgaagtta atggatcaat
     6421 caagagagat gtgggctgta atgaatcgtc ttattgaatt aacaggttgg atcgttcttg
     6481 tcgtttcagt cattcttctt ggcgtggcga gtcacattga caactatcag ccacctgaac
     6541 agagtgcttc ggtacaacac aagtaagctc tgcgcttgta gagcgccatg ctgcccgtcc
     6601 ggttgcatgt tttcacttgt cggatattaa accaggaatt tattatcttg ttcgatgttg
     6661 ttggtgattg tcagggatag taaagcgcgt tactcttttc agagcagttt ttcatccctg
     6721 aatatttgcc atcagcgcat tcttattatt gtaataacac aagcgttcac ttttggtgaa
     6781 attttatcat cactaataat acctacccct ttggtattaa ttattttatg gaccttttat
     6841 gactgttcag gactacttat taaaatttcg caaaatcagt tcactcgaaa gtctggaaaa
     6901 actctacgac catcttaatt acaccctgac ggacgatcag gaactgatca atatgtatcg
     6961 tgctgccgat caccgtcgcg cagagctggt ttctggcggg cgtttgtttg acctcggcca
     7021 ggtaccgaag tccgtctggc actatgtcca ataaagaaag tagcgatcgg ttataaaagc
     7081 cttataataa cgcccctaaa attagatttg cctgtttgcg tcgctattct ggagaaccta
     7141 tgaccaccac aacgccacaa cgtattggag gctggttgct tggcccgttg gcctggcttt
     7201 tagtcgctct gttgagtacg acgctggcgt tgttgctgta taccgctgcg ttatcttctc
     7261 cgcaaacatt tcaaacgctc ggcggacaag cactgacgac acaaatctta tggggcgttt
     7321 cttttattac cgctatcgcc atgtggtatt acacactgtg gttgaccatc gctttcttta
     7381 aacgtcggcg ttgtgttcct aaacactata ttatctggct gcttatttcc gtattgctgg
     7441 cagtaaaagc attcgctttt tcacccgtgg aagatggcat agccgttcgc cagttgctgt
     7501 ttactttgtt ggcaaccgca ttgatcgtct cctatttcaa acgctcgtcg cgggtaaaag
     7561 cgacgtttgt gaatccgtaa taaccttaca gttaacctgt tgtcgcctgc tctggattaa
     7621 cggataatag gcggcttttt tatttcaggc cgaaaaatga ctgactacct gttactgttt
     7681 gtcggaactg tactggtcaa taactttgtg ctggtcaagt ttctcggtct ctgtccgttt
     7741 atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg ggctggcaac aacgtttgtg
     7801 atgacgctgg cgtctatttg cgcctggctt atcgatacgt ggattttgat cccacttaat
     7861 ctgatttacc tgcgcaccct ggcatttatt ctggtgattg ctgtggtcgt gcagttcacc
     7921 gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc tgctggggat ttttttgccg
     7981 cttatcacca ccaactgtgc agtgctcggc gtggcgttgc tgaatatcaa tctcgggcac
     8041 aatttcttgc agtcggcgct gtacggtttt tccgccgctg tcggtttctc gctggtgatg
     8101 gtgctcttca ccgccatccg cgaacgcctt gctgtggctg atgtcccggc accttttcgc
     8161 ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc tggcctttat gggctttagt
     8221 ggtttggtga agttgtaatg aatgctatct ggattgccgt tgccgccgtg agcctgctgg
     8281 gcctggcgtt tggcgccatt ctgggttatg cctcccgccg ttttgcggtg gaagacgatc
     8341 cggtcgttga gaaaattgac gaaatcttac cgcagagcca gtgtggtcag tgcggttatc
     8401 ccggctgtcg cccctacgcg gaagccatca gctgtaacgg tgaaaaaatc aaccgttgcg
     8461 ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt gcttaatgtc gagccgcagc
     8521 cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat ggtggcggtt attgatgaaa
     8581 ataactgtat tggctgcact aaatgtattc aggcttgtcc ggtagacgcc atcgttggcg
     8641 ctacccgtgc catgcatacg gtaatgagtg atctctgtac gggctgcaat ttatgtgttg
     8701 atccgtgccc gacgcactgc atctcgttgc aaccggtcgc agaaacacct gactcctgga
     8761 aatgggatct gaacaccatt cccgtgcgta tcattcccgt ggaacaccat gcttaagtta
     8821 ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg gcggcatcca tccaccggag
     8881 atgaaaaccc agtccaacgg tacacccctg cgccaggtac cactggcgca gcgttttgtt
     8941 attccactga aacagcatat tggcgctgaa ggtgagttgt gcgttagcgt cggcgataaa
     9001 gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa tgctgcctgt tcacgcgccc
     9061 acctcgggta ccgttacggc tattgcgccc cactctacgg ctcatccttc agctttagct
     9121 gaattaagcg tgattattga tgccgatggt gaagactgct ggatcccgcg cgacggctgg
     9181 gccgattatc gcactcgtag tcgcgaagag ttaatcgagc gcatacatca gtttggtgtt
     9241 gccgggctgg gcggtgcagg attcccgaca ggcgttaaat tgcagggtgg cggagataag
     9301 attgaaacgt tgattatcaa cgcggctgag tgcgagccgt acattaccgc cgatgaccgt
     9361 ttgatgcagg attgcgcggc tcaggtcgta gagggtattc gcattcttgc gcatattctg
     9421 cagccacgcg aaattcttat cggcattgaa gataacaaac cgcaggcgat ttccatgctg
     9481 cgcgcggtgc tggcggactc taacgatatt tctctgcggg tgattccaac caaatatcct
     9541 tctggcggtg ctaaacaatt aacctacatt ctgaccggga agcaggttcc acatggcggg
     9601 cgttcatccg atatcggcgt attaatgcaa aacgtcggca ctgcttatgc agtgaaacgt
     9661 gccgttattg atggcgagcc gattaccgag cgtgttgtaa ccctgactgg cgaagcaatc
     9721 gctcgcccgg gcaacgtctg ggcacggctg gggacgccag tgcgtcattt attgaatgat
     9781 gcccgattct gcccctctgc cgatcaaatg gtgattatgg gtggcccgct aatgggcttt
     9841 accttgccat ggctggatgt cccggtcgta aagattacca actgtctgtt ggctccctct
     9901 gccaatgaac ttggcgaacc acaggaagaa caaagctgca tccggtgtag cgcctgtgct
     9961 gacgcctgcc ctgcggatct tttgccgcaa cagttgtact ggttcagcaa aggtcagcaa
    10021 cacgataaag ctaccacgca taacattgct gattgcattg aatgtggggc ttgcgcgtgg
    10081 gtttgcccga gcaatattcc cctggtgcaa tatttccgtc aggaaaaagc tgaaattgcg
    10141 gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag cgcgtttcga agcgcgccag
    10201 gctcgtctgg agcgcgaaaa agcggctcgc cttgaacgac ataagagcgc agccgttcaa
    10261 cctgcagcca aagataaaga tgcgattgct gccgctctgg cgcgggtgaa agagaaacag
    10321 gcccaggcta cacagcctat tgtgattaaa gcgggcgaac gcccggataa cagtgcaatt
    10381 attgcagcac gggaagcccg taaagcgcaa gccagagcga aacaggcaga actgcagcaa
    10441 actaacgacg cagcaaccgt tgctgatcca cgtaaaactg ccgttgaagc agctatcgcc
    10501 cgcgccaaag cgcgcaagct ggaacagcaa caggctaatg cggaaccaga agaacaggt
//
LOCUS       DADLTD010000152        10520 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLTD010000152 DADLTD010000000
VERSION     DADLTD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.152"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            174..1472
                     /gene="kgtP"
                     /locus_tag="JN701_003045"
     CDS             174..1472
                     /gene="kgtP"
                     /locus_tag="JN701_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY7365409.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1469..1813)
                     /locus_tag="JN701_003046"
     CDS             complement(1469..1813)
                     /locus_tag="JN701_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY7365410.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1838..3193)
                     /gene="pssA"
                     /locus_tag="JN701_003047"
     CDS             complement(1838..3193)
                     /gene="pssA"
                     /locus_tag="JN701_003047"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY7365411.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3307..5960)
                     /gene="pat"
                     /locus_tag="JN701_003048"
                     /pseudo
     CDS             complement(3307..5960)
                     /gene="pat"
                     /locus_tag="JN701_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
     gene            complement(5992..6690)
                     /locus_tag="JN701_003049"
     CDS             complement(5992..6690)
                     /locus_tag="JN701_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY7365412.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6759..7178)
                     /gene="trxC"
                     /locus_tag="JN701_003050"
     CDS             complement(6759..7178)
                     /gene="trxC"
                     /locus_tag="JN701_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY7365413.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7385..8422
                     /locus_tag="JN701_003051"
     CDS             7385..8422
                     /locus_tag="JN701_003051"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7365414.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSCAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8470..9159)
                     /gene="ung"
                     /locus_tag="JN701_003052"
     CDS             complement(8470..9159)
                     /gene="ung"
                     /locus_tag="JN701_003052"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY7365415.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9464..9847
                     /gene="grcA"
                     /locus_tag="JN701_003053"
     CDS             9464..9847
                     /gene="grcA"
                     /locus_tag="JN701_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY7365416.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9903..10490)
                     /gene="eamB"
                     /locus_tag="JN701_003054"
     CDS             complement(9903..10490)
                     /gene="eamB"
                     /locus_tag="JN701_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY7365417.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2621 a         2661 c         2705 g         2533 t
ORIGIN      
        1 tcctccttac cagaatttat ccttaagctc ctcaataacc attttcctgc taactaaatt
       61 catggttaat gttgcataat aatacgtaac aaatgtataa cattttcttt accaaaagaa
      121 ataaacaaaa gtgaccaaca aaagcatcgg attacggcag gagacataat ggcatggctg
      181 aaagtactgt aacggcagac agcaaactga caagtagtga tactcgtcgc cgcatttggg
      241 cgattgtggg ggcctcttca ggtaatctgg tcgagtggtt cgatttctat gtctactcgt
      301 tctgttcact ctacttcgcc cacattttct tcccttccgg gaacacgacg actcaactac
      361 tacaaacagc aggtgttttt gctgcgggat tcctgatgcg cccaataggc ggttggctat
      421 ttggccgcat agccgataaa catggtcgca aaaaatcgat gctgttatcg gtgtgtatga
      481 tgtgtttcgg atcgctggtt atcgcctgcc tcccaggtta tgaaactata ggtacgtggg
      541 ctccggcatt attgcttctc gcgcgtttat ttcagggatt atccgttggc ggagaatatg
      601 gcaccagcgc cacctatatg agtgaagttg ccgttgaagg gcgcaaaggt ttttacgcat
      661 catttcagta tgtgacgttg atcggcggac aactgctagc cctactggtt gtcgtggttt
      721 tacaacacac catggaagac gctgcactca gagagtgggg atggcgtatt cctttcgcgt
      781 taggagctgt gttagctgtt gtggcgttgt ggttacgtcg tcaattagat gaaacttcgc
      841 aacaagaaac gcgcgcttta aaagaagctg gatctctgaa aggattatgg cgcaatcgcc
      901 gtgcattcat catggttctc ggttttaccg ctgcgggctc cctttgtttc tataccttca
      961 ccacttatat gcagaagtat ctggtaaata ctgcgggaat gcatgccaac gtggcgagtg
     1021 gcattatgac tgccgcattg tttgtattca tgcttattca accactcatt ggcgcgctgt
     1081 cggataagat tggtcgccgt acctcaatgt tatgtttcgg ttcgctggca gccattttta
     1141 ccgttcctat tctctcagca ttgcaaaacg tttcctcgcc ttatgccgct tttggtctgg
     1201 tgatgtgtgc cctgctgata gtgagttttt atacatcaat cagtggaata ctgaaggctg
     1261 agatgttccc ggcacaggtt cgcgcattag gcgttggtct gtcatatgcg gtcgctaatg
     1321 ctatatttgg tggttcggcg gagtacgtag cgttgtcgct gaaatcaata ggaatggaaa
     1381 cagccttctt ctggtatgtg accttgatgg ccgtggtggc gtttctggtt tctttgatgc
     1441 tacatcgcaa agggaagggg atgcgtcttt agtgacgggt cagttgccag acggtatagc
     1501 cggtgcttgc accggcgaca tcccaggcca aatccttcca gctccagccg ctcccttcgg
     1561 ggcggctatc ccaaagctct tttgacgccc ccaaactgac agagaacatc aatccaaaca
     1621 tggcactgcg atcccggctc atcccctgat gctgtgaata ttcatttccg gcggcggaaa
     1681 gcatcgccga ggcgataaag tgttgagctt tatcctgccc gctccagcta tcgttagcca
     1741 tatggctgca tccagaaagt aacaatagcg aacagacaaa aagaatacgc aaaacgtggc
     1801 ctccaaaaaa caaaccccgt acaggacggg gttgtgatta caggatgcgg ctaattaatc
     1861 ggtcgatgcg gatacggcgc aaacggcgga tgagtttacg aaccttcacc ggataatcgg
     1921 caatactttg cagatcgcga tagtgcttaa cgatggtggt atgctcgcgg atcagctcca
     1981 gttctttctc tcgctgcggc gcaagctcca gttgtggatc gtggatcaaa atggcgtttt
     2041 ccagatccag acgccaggcg cgcgggttca ggttattacc ggtgatcaac atccacttat
     2101 catcaaccca catccctttc aggtgatagg tgttgtcgtc atctttccat aaccgaacca
     2161 ctagctggtc agtattgacg taatactgca aacggctcag gaaaagacgc agattgatct
     2221 catagagata aggcaatgcg ccaattatct tgaaaggttc atcttccgga atgtagaagt
     2281 cgttcgcggt tttatcacca acaataattt cgaccttttt cccttcgcgc agcaactgga
     2341 taatattgcg cacaaggatt gctggcaggt tgaagtatgg cgtacagatg gttagtttct
     2401 gctcggcaca aggcataaga tggaaaatgg tcttgttcaa cagactcgat ttccccagcc
     2461 ccactagcgg cgttacggaa agctgatcgt tgtcggcatc gccctggaaa tgataagcgg
     2521 catcacgcag ctcctggcgg aacagacgaa tatcgttctt gatttccggg ctttttggcc
     2581 gattaacatc atccagacga ttaacgccgc ggccattcat aatattctgt gtaacccatt
     2641 caaacataat gtctgacatc ttacggttac ggataagatg ataacggtcg tagcgatatt
     2701 tatcgtgctg atgcaggtaa acatcgttca ggctggcacc gctataaagt acgctatcgt
     2761 cgatgataaa gcctttaaag tgcagaacac caagggcttc acgggtattg attggaacgc
     2821 cataaaccgg aacatctacg cccgaatttt cctgcgccat gcggcagtac cagtcagcgt
     2881 tagtgttaga tgccgcagcg ccaatgcgtc cacgttgtgc acgatgccag tcgaccagca
     2941 cccgcacatc cagttccgga cgctgccttt tagctttata caacgcgttc agaatgcctt
     3001 tgccaccgtc atcctgttcg agatacaggg cgacaatgca aatgcgctgc ttcgcgctgg
     3061 ctattttttc cagcagcgtc tcccggaagt cggcgggagc gtaaaagaaa tcgacatcat
     3121 caactgattg agaaatcttg ggtagttggg caaggtgttg ttgatgttta ttacgcttaa
     3181 attttgacaa catcacagtg catttcttct ctgttcattg aaggatcctc tgtgcaatgc
     3241 agacgacata agcgggcaat aataacacca gtcccgatta agtagtcaac atttccagta
     3301 ccttactcat gattcctcgc gttgggcaag atttagcgta agcccaacga tcccctcttc
     3361 gagctggata tcaacgttaa acccgagctt gcgggttagc gccaccatgc cacgattgtt
     3421 tggcatcgta ataccattca gacgttgtag tccgtgatct cgcgtatagg taatcaactt
     3481 ttccattaag cgtcgaccta agcctaaccc tttgagatcc gagcgaacca gcacagcaaa
     3541 ttcggcatcg atgttatcag gatcggaaat cgcacgcgtg acgccgagga tctcttccgt
     3601 ttgatcaata cgtcgtaccg ctacaaacgc catttcccga tcgtagtcga tctgtgtcat
     3661 gttggctaaa tcttcatggg taaattcgtt gatctcgcta aagtagcggt aataaagatc
     3721 ttctttggtg actcgcgaaa tgaattgctg aagttgtggc tcatcttctg gcaaaatcgg
     3781 gcggaacaag cagcgttcac cgtttttcaa ttctacccat tcttccagct gatgcggata
     3841 agggcgcact gccagccgac tctcgttatc gccttcaaac ggcgagatat ccagcgtgac
     3901 atccagcgcg gtaaattcac tgccagaagc cagcaaagga tgaatatcca gacgctgaat
     3961 ttccgggcaa tcgacaatca agttggaaac ctgcaccaga agctggctca agcctgcaac
     4021 atccaatggg cgtagcgcac tgcgcgcacg aatcttttta cttttgatcc cctgaataac
     4081 cagatagcgg gccaggttca tgttcagcgg cggcagtgcg acgacggctt gatcttcagg
     4141 acgccactcc acaccgcctt cacccagcat gatcaacggc ccgaaaaccg gatcgtgccc
     4201 aaccacaacc cgcaactcct gagcgccagc acggttagcc atactttgca ccaacaggcc
     4261 gtggatccgc gcctgcggcc aggccatttt tacgcgatcg aaaatggcgt ttgccgcttg
     4321 ctggacttca ttggctgtac gcaagtacag catgacgccc tgaacttccg atttatgtgg
     4381 aatatccggc gaacgcaatt tcagcgccac cggataacca atctgttcgg caatatgcac
     4441 cgcttcggtg ctatcgctgg caatccaggt agggagcgtg ttcatgccat acgcttgcag
     4501 gatgggctga acttcatggg tatcgagcga cgtagcccct tcggcaatcg cctgttgcaa
     4561 cagaagatgc gcttctgcgg tattggaagt cagattgctg ggcaacgccg gcgtttcgcg
     4621 tagttgcttc tgattacgcc ggtactccac catatgcata aaagcagtga tggttccttc
     4681 cggggtacgg taggtcggca gcccggcttc gctgaataaa cgtcgtgcct cttgcgagga
     4741 gtgctcgccg caccagttcg tcagcaaaga gacatatttg ctgcggggat gatgctttac
     4801 cgcttcaatt aatacttgcg cgctttctgt tgcgggagca gcggcgctgg gcgaatgaat
     4861 aaccatcagc gcgtcaaaat cctggctgtg gagcagaata tccagcgttt taatatagtg
     4921 ctcactgctg gcgtcatcgc gtagatcgag cgggttagat attgccacat gttctggcag
     4981 tgcatcgcgc agtttctggc aggtttcttc gcttagcgtt gccagcttgc cattgcgtga
     5041 ccataaggca tccagcgcca gcgcggcagg cgcagcaccg ttgctgataa tcatcagccg
     5101 gtcgccacgt agcgggcgca tatggctaag ggtttccacc gccgaaaaca gctcgtgggt
     5161 gtcctgtacc cgcaacaaac cggcacgctg aatagccgca tcccatgccg ggtccattcc
     5221 tgccgtggtg ttgagcagtc gctgtgccgc cgggctacgt ccgcttttaa tcaccagaat
     5281 cggtttatta cgcgaggcac tacgggccgc cgaaacaaag cgtcgcgcgt cgcttaactg
     5341 ttcgagatag agcaggatgg cgctggtttt actgtcgcgt gccagatagt caagcaattc
     5401 atcaacgtcg atatccaggc tgtcgccgag cgcaataaag taggaaaagc ccatcttacg
     5461 ctgttgcgcc cagtcgagga tggtgttgga gacggcagcc gattgcgaaa taaacgccag
     5521 cttgccgcgt ttaatcggca caggcgaaaa gctggcattc agaccttgcc agggagccag
     5581 taaacccaga ctgtttggtc caagcaggcg catgttatgg cgcaggcgcg gagatcttcg
     5641 tgttgcgatg ccggggcgga aagaataatg caggttttac agcctttctc gccgagctct
     5701 tccagaagag caagattacg gctggcattg gtacataaaa ccgcaaggtc gggtgtaaag
     5761 ggcaagctgg caatatccgg ccaggccaac acacccaaca ccgctttcca ggctggcgtc
     5821 accgggagta ccggtccgtt aaagcctccc gccagcaggt tacgcatcat caggtaacct
     5881 gcgcgattgg gtttcatcga cgcgccaatt accgctatcg attttggtcg cagtagtgct
     5941 tccagtcctc gctgactcat accggtctcc cgcagaagtg accgaatgat tttaaacgct
     6001 ttctaactgt tctgctgtga tgctacccag atgttgcgtt ttccctgcca gatagcgtgt
     6061 tttaaagcgg gtaaaatgct cgcctaaccc tgctgccgcc ccggtatcgc cggccatatc
     6121 taacagtgcg atggctacct cggcagtaca atattggcct tcagccaggg cttcacgcag
     6181 gcgataggca gaaagccggg aaagatcgac ggaaatgacg ggaagattat ccagatacgg
     6241 acttttacga aacatcttgc gagcttccgg ccaggtacca tcgagcatga taaacagcgg
     6301 tggcttaccg gcaggtggtg tgaagatcac ttcccgttgc tcatcagcat acgaggcggg
     6361 aaagaccacc attggctgat aatccgggtt ttgtaccaga tccagcaaat cctgcgaggg
     6421 ttcggtacgc gaccattgaa acgcaacggt atcaggcaaa atatcagcaa tcagacgccc
     6481 ggtattactg ggcttcattg gctcggtgtc gaacatcagc aaacagaagc gactttttgc
     6541 ttgtgctggg gtaattgtcg aacagagaca taatttctct ggcaaaagac agcgttggca
     6601 gcgacgaacg cgattaccgc gggcaagaaa aggacgtgtt gcgcgcgcaa tacgctcggc
     6661 gcgtaactgg agaacagtgt tttcggtcat aagagagcgt cgaaaaaacg ccattgtcgc
     6721 agaggagaaa acggggcaca agatgcgccc cggtaagatt aaagagattc gttcagccag
     6781 ctatcgaacg gagctttcgg tactgcgcca ttaagcatgt cgacaacctg accgtttttg
     6841 aaaatcatga tcgtcggtat actacgaatt ccaaagcgac tgctcaattc acgttcagct
     6901 tcggtattca ctttcacaaa gcgcacttta ccgctacgct cttgcgcgac atcttcaaaa
     6961 attggtgcga aattacggca ggggccgcac cacggtgccc agaagtcgat caccacaggt
     7021 agatcatcct tcagcaattt gtcgagtgtt tcaccggtcg cattaatcac ctctccgtca
     7081 aacaagtcgt gaccgcagcg tccgcatttt gccgcatctt cgatccgatc gtcgggaatg
     7141 cgattgatgg actgacaatg ggtacaaacg gtattcataa ctaacctcgg gatgagtaag
     7201 gagacttatc ggcaacgacg ccggtatgtt tctaatatgt tacatattat cggggacatt
     7261 gttccagaca acaatctgtt ttattcaata ggtatgatag tcgcaggctt tggtacgaaa
     7321 tcatggagaa ccgcgcggac ttcgggtaat ctgcgcgctt cgcgcagcgc tggtggagaa
     7381 aagcatgaac gatgaaatga aaggtaaaag cggcaaggtc aaagtgatgt atgtccgcag
     7441 tgatgatgat tctgataaac gtacccacaa cccgcgtacc gggaaagggg gcgggcgtcc
     7501 aggaaaatct cgtgctgacg gtggccgtcg ccccgcccgc gatgacaaac agagtcagcc
     7561 ccgtgaccgc aagtgggaag attcgccgtg gcgcacggtt tcctgcgcgc cgggtgatga
     7621 gacgccggaa aaggccgatc acggtggcat cagtggtaaa agttttattg atccggaagt
     7681 gttgcgtcgt cagcgtgcgg aagaaacccg cgtctacggc gaaaacgcct gtcaggcact
     7741 gttccagagc cgtccggaag caattgttcg cgcctggttt atccagagcg taacgccacg
     7801 ttttaaagaa gccttgcgct ggatggcggc aaaccgcaaa gcgtaccatg tggtggatga
     7861 agctgaattg acaaaagcgt caggcacgga acatcacggc ggcgtttgct tcctgatcaa
     7921 aaagcgtaac ggtacaaccg tgcagcagtg ggtaagccag gcaggcgcgc aggattgcgt
     7981 tctggcactg gaaaacgaat ctaacccgca taacctgggt ggcatgatgc gcagctgcgc
     8041 gcacttcggc gtgaaaggtg tcgtggtgca ggatgcggca ctgctggagt cgggggcggc
     8101 tatccgtacc gcagaaggcg gcgcagagct cgttcagtcg attactggcg acaacattgt
     8161 taacgtgctg gatgatttcc gtcaggcagg ttacaccgta gtgaccactt ccagcgagca
     8221 gggtaaaccg ctgttcaaaa ctagtctgcc agcgaaaatg gtactggtgc tgggtcagga
     8281 atatgaaggg ttaccggatg ccgcacgcga tccgaacgat ctgcgcgtga agattgacgg
     8341 tacgggcaac gttgccgggc tgaatatctc tgtcgcaacc ggcgttttgc ttggtgaatg
     8401 gtggcgtcag aataaagcct gataaatcag ccgggtggca actctgccat ccggcatttc
     8461 cccgcaaatt tactcactct ctgccggtaa tactggcatc cagtcaatcg gcgtctcgcc
     8521 acgttgttcc agccactgat ttgccagcac aaaatggttg cagccaaaga atccacgatg
     8581 cgccgaaagc ggcgacggat gcggtgcttt cagtacatga tggcgttgct tatctataat
     8641 cgcccctttc ttttgcgcat gtgatcccca caacaaaaac accacgcctt cgcggtgctg
     8701 gttaatcagg ctgatgactt tatcagtaaa cgtctcccaa ccgaggctgg cgtgggaatg
     8761 cgcctgacct gcgcgaaccg tcaacacagt attgagcagc agaacgccct gacgcgccca
     8821 gctttcgaga tagccatgat tagggcgggt gaagcccgga atagtatttt ccagctcttt
     8881 atacatattc aataatgacg gaggaatggc aatgccggga cgaacggaaa atgccagacc
     8941 atgcgcctgt cccggtccgt gataaggatc ctggccgaga atcaccactt taacgtcacc
     9001 caactcggta aagcggaacg cgttaaagac atctttttgt ggtggataga tagtgacgcc
     9061 ggactgccgc tcgctggcga cggtctgaag ggtattaaga aaataaggtt gttgcttctc
     9121 ttcagccagc acgtcatgcc aggttaattc gttagccatc tcgctctcct gcgaatcttc
     9181 aatccgccta gcttaactgc ttctttctac agaacaaaat catgctcatt ttttagactt
     9241 aaacgagtgc aaaaaaatgt aaattggtaa aatttcacac ccgcataagt tgatgtaaaa
     9301 caataaaatc catttacgac cacaatcgat gtgtggtttt tattgattta aatcaaagaa
     9361 tcaagggtgg ttgaggagta tatatacact caagcaacaa tggttttacc aattagccgc
     9421 gacaggctga acaaatcaaa taattttgcc ggggaggcat cacatgatta caggtatcca
     9481 gattactaaa gccgctaacg acgatctgct gaactctttc tggctgctgg acagcgaaaa
     9541 aggcgaagca cgttgcatcg ttgcaaaagc aggttatgca gaagatgaag tggttgcagt
     9601 aagcaaactg ggtgacattg aataccgtga agttccagta gaagtgaaac cagaagttcg
     9661 cgttgaaggt ggtcaacacc tgaacgttaa cgttctgcgt cgtgaaactc tggaagatgc
     9721 agttaagcat ccggaaaaat atccgcagct gaccatccgt gtatccggtt atgcagttcg
     9781 ctttaactct ctgactccgg aacagcagcg cgacgttatc gctcgtacct ttactgaaag
     9841 cctgtaaggc ttaagtagca agttactaag cggaagaggg cgacctcttc cgcttttttt
     9901 cgttaataga aaatgcgtac cgcgcaatag accagcaaca gggcaagcac gatatttaac
     9961 tggcgaccat actggcgaaa caatcgctga aacagatgcc ccgccagcgc ccagcacaca
    10021 ttgccaaacg tcccaatcat cgccagcaaa acgctgacgc caactaccca gcttagcgcc
    10081 tgtgtttgcg gcagaacaaa cgtcgacagt gccgtaacac cgtacaaaat gattttgacg
    10141 ttcacaaact gcaaagcaaa gctggcccaa aagctgattg gttttgcctg aagtccgtct
    10201 tcctttgttg ggctggtggc gattttccac gccagccaga caatatatgc cgcccccgcc
    10261 caactcaaaa ggtgtaccgc tgccgggtca atcactgcca gtgaaaatga aatgcccgca
    10321 cacagtaaca tcacaatcaa aaatcccaga ctcatccctg ccagcacgcg ggtactttga
    10381 cgaaatccat gcgacgtagc agagctaagg gcgagaatat tgttcggtct tggcgtcata
    10441 gcggtaatca gggtgtaagt ccaaaaagca cttaaaaggg tcggtgtcac ttgttatctc
    10501 ctctgttttg gtagacagta
//
LOCUS       DADLTD010000153        10440 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLTD010000153 DADLTD010000000
VERSION     DADLTD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.153"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(4..1902)
                     /locus_tag="JN701_003055"
     CDS             complement(4..1902)
                     /locus_tag="JN701_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7365418.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSELINQFARQLITLLD
                     TSDLSSMLSYCITQEFTANCRKISQNCYSTVLFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQLVYGQ
                     LNYVASVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNALTTPIAQELLNKKIVMDILAMKTRDGELGLFAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAHGTPVLYIAMSKGNEDVVLSYISTL
                     NIFAKKYSFSQRQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            2424..4352
                     /gene="thrS"
                     /locus_tag="JN701_003056"
     CDS             2424..4352
                     /gene="thrS"
                     /locus_tag="JN701_003056"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY7365419.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4356..4898
                     /gene="infC"
                     /locus_tag="JN701_003057"
     CDS             4356..4898
                     /gene="infC"
                     /locus_tag="JN701_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY7365420.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4995..5192
                     /gene="rpmI"
                     /locus_tag="JN701_003058"
     CDS             4995..5192
                     /gene="rpmI"
                     /locus_tag="JN701_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY7365421.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5245..5601
                     /gene="rplT"
                     /locus_tag="JN701_003059"
     CDS             5245..5601
                     /gene="rplT"
                     /locus_tag="JN701_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY7365422.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            5724..5768
                     /locus_tag="JN701_003060"
     CDS             5724..5768
                     /locus_tag="JN701_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY7365423.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6051..7034
                     /gene="pheS"
                     /locus_tag="JN701_003061"
     CDS             6051..7034
                     /gene="pheS"
                     /locus_tag="JN701_003061"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY7365424.1"
                     /translation="MSHLAELVASAKAAISQASGVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7049..9436
                     /gene="pheT"
                     /locus_tag="JN701_003062"
     CDS             7049..9436
                     /gene="pheT"
                     /locus_tag="JN701_003062"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY7365425.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9441..9740
                     /gene="ihfA"
                     /locus_tag="JN701_003063"
     CDS             9441..9740
                     /gene="ihfA"
                     /locus_tag="JN701_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY7365426.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10044..10184)
                     /locus_tag="JN701_003064"
     CDS             complement(10044..10184)
                     /locus_tag="JN701_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HAY7365427.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2741 a         2363 c         2664 g         2672 t
ORIGIN      
        1 tggttataaa tacgtcttta gttcatcagc actaaaactc aggctttggc tgattacatt
       61 tatagcagca taatatgttt ctacagtttt ataatgatta tgatgaatgg ctatatgaac
      121 agctgacatg ttgtcatgat ttttagcggc caacagtgtg aataactgac gttgactaaa
      181 agaatatttt tttgcaaaaa tattcagcgt cgatatataa gataacacga cgtcttcatt
      241 acccttgctc atggcgatgt ataatacagg ggttccatgt gctgtagcgc cttttaataa
      301 atccatgatg ttagctttgc tcaacttgta tttaaaggcg atgccattaa ttttagagag
      361 gaaccgtgtg acacacaaag ggtgattatt ttccattgca gcaaacaacc ctagttcacc
      421 atcacgtgtt ttcattgcta aaatatccat cactattttt ttgtttaaaa gctcttgtgc
      481 tattggagtg gttaatgcat tcagaaagat atcgattgtt tcatcgaacc catacaacat
      541 ggagatatag agcccaggtg tttcgttagc acttttagtt tgcagtaatt taaccagatt
      601 atcttcatga atgatatgat tgtatatcag tgactgaatt tcttgcataa atatttttac
      661 gttgtcagta tgtccgttca tcatagccag aaataaagca ggtgtaccaa cgctattctg
      721 cgcagttatt atttcaatga tttctttttc agggagtatg ggcaactgtt tgaataatgt
      781 aataagctgt tgagtctggc ctgtggccat tattaacaac attgccgatg gatgtatagg
      841 ggcaaatggc attcgtgatg caatacccgc gaaaatagcc cctggaactc ccacaggtaa
      901 taggggcaga atagcaattg cacagttgcg aacataccta tcagcccagg tttgagcatc
      961 gggacctgaa ctgatgaact tatcaagcga aagaaagccc cgtgctgtcc tgtgggtgcc
     1021 tctgacggca acgttggtat cgttcgggtc gtagacggac gctacatagt ttaattgtcc
     1081 atacactaac tgctttattc gtagccttac tgacaaagta tgattgtcta tatataaaat
     1141 cgcggacata tgactgacat tgtttgccat cattcgacaa aagaaaatat ctaaagcgaa
     1201 gccaaaatcc ttactcgcga taacgcgact ccctgcagga ggatggcgaa gtaatgctct
     1261 gaactccgca tcccggacta aggggacatg ctgctgtatg gattcaacgg aggagtaatg
     1321 tttaatcagg ttgactttgc cttttccatt tctcaatgaa tcaagggcgt attgcaatga
     1381 cagatggcga caaaaaatag cgtcagaagg agattgcaaa aaacatgcac taccattgag
     1441 atttactgtt ggttgtatag ttttttctct atggattttt ttagacagtt tcatacaatg
     1501 gttacgatct tcggcagtta actgttcctg ctcaatataa tccagtatgc ttcgcaaatg
     1561 gttagcctta ataggcgtgg tttccagaaa taagggaatg atttcttttt tatagtgtaa
     1621 tgtaataagt atattttctg catggatggg tgaagtggca aagttaatga taaaaaggac
     1681 agtggagtaa caattttgtg atatttttcg acagtttgcg gtaaattcct gagtaataca
     1741 gtatgacagc atcgaactta agtcagaggt atcaagtagg gtgatgagtt ggcgcgcaaa
     1801 ttgattaata agctctgatg aaggcgttgg tatgtcgcta tcgatgtgca atctatgagg
     1861 agacttataa tgagttctga taatgatatc gttttgactc ataatctatt caccttatat
     1921 atactggagg ttttgaggat gatgattaat tattatcgcc agaacgaaat gtgcacgaaa
     1981 aattgctata agaattgtgt cacttttatc attgtgatat gaccacttcg tcattaatct
     2041 catgatcgtt taaatactac cgccaaatca cgcaacgaac tttgctggat tcagtttatg
     2101 ctgctgtaaa tccgctcgca gtaaaccttt cagacgcacc gtgatgttat cagtggttct
     2161 ttaagcgttt tgctggtgta ctcactacaa acgaattgcg aatcgaatca atgtgaaacg
     2221 gaaaggtaca atctcccttt ttttagtgtt aacatcgttc aaccgggtgg aaggtgagag
     2281 gttaatgtct ttcatttgtg tacctgattg cgaaccaatt tagcatttgt tggctagatg
     2341 gtttcgcaat gaactgttaa taaacaaatt tttctttgta tgtgatcttt cgtgtgggtc
     2401 accactgcaa ataaggatat aaaatgcctg ttataactct tcctgatggc agccaacgcc
     2461 attacgacca cgctgtaagc cccatggatg ttgcgctgga cattggtcca ggtctggcga
     2521 aagcctgtat cgcagggcgc gttaatggcg aactggttga tgcttgcgat ctgattgaaa
     2581 acgacgcaca actgtcgatc attaccgcca aagacgaaga aggtctggag atcattcgtc
     2641 actcctgtgc gcacctgtta gggcatgcga ttaaacaact ttggccgcat accaaaatgg
     2701 caatcggccc ggttattgac aacggttttt attacgacgt tgatcttgac cgcacgttaa
     2761 cccaggaaga tgtcgaagca ctcgagaagc ggatgcatga gcttgctgag aaaaactacg
     2821 acgtcattaa gaagaaagtc agctggcacg cagcgcgtga aactttcgcc aaccgtgggg
     2881 agagctacaa agtctccatt cttgacgaaa acatcgccca tgatgacaag ccaggtctgt
     2941 acttccatga agaatatgtc gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct
     3001 gccatcattt caaactaatg aaaacggcag gggcttactg gcgtggcgac agcaacaaca
     3061 aaatgttgca acgtatttac ggtacggcgt gggcagacaa aaaagcactt aacgcttacc
     3121 tgcagcgcct ggaagaagcc gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc
     3181 tgtaccatat gcaggaagaa gcgccgggta tggtattctg gcacaacgac ggctggacca
     3241 tcttccgtga actggaagtg tttgttcgtt ctaaactgaa agagtaccag tatcaggaag
     3301 ttaaaggtcc gttcatgatg gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact
     3361 acaaagatgc aatgttcacc acatcttctg agaaccgtga atactgcatt aagccgatga
     3421 actgcccggg tcacgtacaa attttcaacc aggggctgaa gtcttatcgc gatctgccgc
     3481 tgcgtatggc cgagtttggt agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc
     3541 tgatgcgcgt gcgtggattt acccaagatg acgcgcatat cttctgtact gaagaacaaa
     3601 ttcgcgatga agttaacgga tgtatccgtt tagtctatga tatgtacagc acttttggct
     3661 tcgagaagat cgtcgtcaaa ctctccactc gtcctgaaaa acgtattggc agcgacgaaa
     3721 tgtgggatcg tgctgaggcg gacctggcgg ttgcgctgga agaaaacaac atcccgtttg
     3781 aatatcaact gggtgaaggc gctttctacg gtccgaaaat tgaatttacc ctgtatgact
     3841 gcctcgatcg tgcatggcag tgcggtacag tacagctgga cttctccttg ccgtctcgtc
     3901 tgagcgcctc ctatgtgggc gaagacaacg agcgtaaggt accggtaatg attcaccgcg
     3961 caattctggg gtcgatggaa cgtttcatcg gtatcctgac cgaagaattc gctggtttct
     4021 tcccgacctg gcttgcgcca gttcaggttg ttatcatgaa tattaccgat tcacagtctg
     4081 aatacgttaa cgaattgacg caaaaactat caaatgcggg cattcgtgtt aaagcagact
     4141 tgagaaatga gaagattggc tttaaaatcc gcgagcacac tttgcgtcgc gtcccatata
     4201 tgctggtctg tggtgataaa gaggtggaat caggcaaagt tgccgttcgc acccgccgtg
     4261 gtaaagacct gggaagcatg gacgtaaatg aagtgatcga gaagttgcaa caagagattc
     4321 gcagccgcag tcttaaacaa ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa
     4381 cggcgcgccc taaccgtatc aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc
     4441 tggaaggcga gcagcttggt attgtgagtc tgagagaagc tctggagaaa gcagaagaag
     4501 ccggagtaga cttagtcgag atcagcccta acgccgagcc gccggtttgt cgtataatgg
     4561 attacggcaa attcctctat gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa
     4621 gagttatcca ggttaaggaa attaaattcc gtcctggtac agatgaaggc gactatcagg
     4681 taaaactccg cagcctgatt cgctttctcg aagagggtga taaagccaaa atcacgctgc
     4741 gtttccgcgg tcgtgagatg gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga
     4801 aagacgattt gcaagaactg gcagtggtcg aatccttccc aacgaagatc gaaggccgcc
     4861 agatgatcat ggtgctcgct cctaagaaga aacagtaagg ccttcaagta gcactgtctg
     4921 tggagccttc gggcttcaca ggctttgttc gcctttgttt cgtttattaa caatgcgaag
     4981 tggaagttat taaaatgcca aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa
     5041 aaaccggtaa aggtggtttt aagcacaagc acgctaacct gcgtcacatt ctgaccaaaa
     5101 aagcgaccaa acgtaaacgt cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg
     5161 gcctggtaat cgcgtgcctg ccgtacgcat aagccgttaa cgtttttaac tttttaatta
     5221 gaatatagat acaggagagc acatatggct cgcgtaaaac gtggtgttat tgcacgcgca
     5281 cgtcacaaga aaattttgaa acaagctaaa ggctactacg gtgcgcgttc tcgcgtatac
     5341 cgcgttgcct tccaggctgt tatcaaagct ggtcagtatg cttaccgtga ccgtcgtcaa
     5401 cgtaagcgtc agttccgtca actgtggatt gcacgtatca acgcagcagc acgtcagaac
     5461 ggtatttctt acagcaaatt catcaatggc ctgaaaaaag cctctgttga aatcgaccgt
     5521 aagatcctgg ctgatatcgc agtattcgac aaagtagcgt tcaccgctct ggttgaaaaa
     5581 gcgaaagcag ctctggcata agccagttga aagagggagc tagtctccct cttttcgttt
     5641 caacgccatc aaaacattga cttttatcgc cgtagccttt tcaataaagg tcttttgaag
     5701 agtaccaaaa ggtaacgcaa gcaatgaatg ctgctatttt ccgcttcttt ttttacttta
     5761 gcacctgaat ccaggaggct agcgcgtgag aagagaaacg gaaaacagcg cctgaaagcc
     5821 tcccagtgga ggcttttttt gtatgcgcgt tttgaaaaat aaaaagccag tctatcaggc
     5881 gtaattgtcg cagccagtct ggatacggcc agcgcggaga aaccggagcg tatttaagta
     5941 cgtgagaatt tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggata
     6001 ggctctaagt ccaacgaacc agtgtcacca ctgacacaat gaggaaaacc atgtcacatc
     6061 tcgcagaact ggttgccagt gcgaaggcgg ccattagcca ggcgtcaggt gttgccgcgt
     6121 tagataatgt gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga
     6181 ccctgcgtga gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga
     6241 aagagcaggt tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gcagcgctga
     6301 atgcgcgtct ggcggcggaa acgattgatg tctccctgcc aggtcgtcgc attgaaaacg
     6361 gcggtctgca tccggttacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg
     6421 gctttaccgt ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga
     6481 acattcctgg tcaccacccg gcgcgcgctg accacgacac tttctggttt gacactaccc
     6541 gcctgctgcg tacccagacc tctggcgtac agatccgcac catgaaagcc cagcagccac
     6601 cgattcgtat catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc
     6661 cgatgttcca tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga
     6721 aaggcactct gcacgatttc ctgcgtaact tctttgagga agatttgcag attcgcttcc
     6781 gtccttccta cttcccgttc accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg
     6841 gtaaatggct ggaagtgctg ggttgcggga tggtgcatcc gaacgtgctg cgtaacgttg
     6901 gcatcgaccc ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacta
     6961 tgttgcgtta cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca
     7021 aacagtttaa ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg
     7081 ggtgaacccg gcgattgata gcgatgcgct ggcgaatcaa atcactatgg cgggcctgga
     7141 agttgacggt gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtggt
     7201 tgagtgtgcg cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg
     7261 cgatcgcctg ctggacatcg tctgtggtgc gccaaactgc cgtcagggcc tgcgtgtggc
     7321 ggtagctacc attggtgctg ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg
     7381 tggcgaaccg tctgaaggga tgctgtgctc cttctctgaa ctgggaattt ctgacgatca
     7441 cagcggcatt atcgaactgc ctgcagatgc gccgattggc actgacatcc gcgaatacct
     7501 gaaactcgat gacaacacca tcgaaatcag cgtaacgcca aaccgtgccg actgcttagg
     7561 tatcattggt gttgcgcgtg acgttgccgt gctgaatcag ctgccgctgg ttgaaccgga
     7621 aatcgttccg gttggtgcga ccatcgacga cacgttgccg attacagtcg aagcgcctga
     7681 agcctgcccg cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc
     7741 gctgtggatg aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga
     7801 cgtcaccaac tatgtactgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg
     7861 cattgaaggc ggcattgtgg tgcggatggc aaaagagggc gaaacgctgg tgctgcttga
     7921 tggcactgaa gcgaagctga atgctgacac tctggtcatc gccgaccaca acaaggcgct
     7981 ggcgatgggc ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt
     8041 cttgctggaa tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg
     8101 cctgcatact gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa
     8161 agcgatggaa cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt
     8221 aattgatatc accaacgaag caacgctgcc gaagcgtgca accatcactt tacgtcgtag
     8281 caaactggat tgcctgatcg gccatcatat tgctgatgaa caggtaactg acattctgcg
     8341 tcgtctcggc tgcgaagtga ccgaaggcaa agacgagtgg caggcagttg cgccgagctg
     8401 gcgtttcgat atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta
     8461 caacaacatc ccggatgagc cggtacaggc aagcctgatt atgggtactc accgtgaagc
     8521 tgacctgtcg ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc aggaagtgat
     8581 cacctacagc ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt
     8641 actgctgcca agcccgatct ctgttgaaat gtcagcaatg cgtctttctc tgtggaccgg
     8701 cctgctggca accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga
     8761 aagcggtctg cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat
     8821 gttagccggt gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac
     8881 cgttgatttc tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact
     8941 gaatgaggtt gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc
     9001 gatttatctg aaaggggaac gtattggttt tgttggagtt gttcatcctg aactggaacg
     9061 taaactggat cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga
     9121 ccgcgtggtg cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc
     9181 ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg
     9241 cgtaaatcag gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga
     9301 ggggtataag agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga
     9361 agaggagatt gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc
     9421 atcattgagg gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg
     9481 ataagcttgg gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga
     9541 tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc
     9601 tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta
     9661 cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg
     9721 cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct
     9781 ttcaataact aattgattta taatgtttta tatctcttcg ttccacgatg cgtccacatt
     9841 tagtgggggg aggggagtaa gcccgctaca ttgtgggctt tgtcttatag tctgatcact
     9901 gtttcaagtc ggtgaggaca ccaaaaagaa cccccgcagt ttttacgctg cgggtttgtt
     9961 gttcatgtct gtgagatagg gtgccttatc gaccttaccc tggcaaccga ttgacggggg
    10021 attgctcccc cgtcgcggtt tccttactgc ttacactgta agaacgccgc aaactccgct
    10081 ccccagaagc tcatccgtat ttcgcacagc gaaccgtgca gcatccagat gatgaggatt
    10141 gccgtcacgc agaacgtgat ggccgtaagc gatttttgcg acatagcgct tgctcctttt
    10201 tcggggaggc gctaacctat cacttgctta gggtagacgg ttagggcctc ggttaaacaa
    10261 aaatgttttc cggggccttt ccacatctgg ccttcaggta ttccctccga ccatcagccg
    10321 aaaggcaccc gcgcgtaatc tatcgctttt ttgttactcc ggcaattctg ctcgttaatt
    10381 ctgaggtaag ggcaaactca tctgattgtt tcctctgtgt gaagttggca gttcatgcca
//
LOCUS       DADLTD010000154        10414 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.154, whole genome shotgun sequence.
ACCESSION   DADLTD010000154 DADLTD010000000
VERSION     DADLTD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.154"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..619
                     /locus_tag="JN701_003065"
     CDS             <1..619
                     /locus_tag="JN701_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7365428.1"
                     /translation="GQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTELGSRIICRDKQL
                     IFEEAPQAYKSAESVVQCLGQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            616..1230
                     /locus_tag="JN701_003066"
     CDS             616..1230
                     /locus_tag="JN701_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAY7365429.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1287..2744)
                     /gene="pepD"
                     /locus_tag="JN701_003067"
     CDS             complement(1287..2744)
                     /gene="pepD"
                     /locus_tag="JN701_003067"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7365430.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3005..3463
                     /gene="gpt"
                     /locus_tag="JN701_003068"
     CDS             3005..3463
                     /gene="gpt"
                     /locus_tag="JN701_003068"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7365431.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3555..4799
                     /gene="frsA"
                     /locus_tag="JN701_003069"
     CDS             3555..4799
                     /gene="frsA"
                     /locus_tag="JN701_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7365432.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4857..5258
                     /locus_tag="JN701_003070"
     CDS             4857..5258
                     /locus_tag="JN701_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7365433.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5297..6352)
                     /gene="phoE"
                     /locus_tag="JN701_003071"
     CDS             complement(5297..6352)
                     /gene="phoE"
                     /locus_tag="JN701_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7365434.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6640..7743
                     /gene="proB"
                     /locus_tag="JN701_003072"
     CDS             6640..7743
                     /gene="proB"
                     /locus_tag="JN701_003072"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7365435.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7755..9008
                     /gene="proA"
                     /locus_tag="JN701_003073"
     CDS             7755..9008
                     /gene="proA"
                     /locus_tag="JN701_003073"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7365436.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9123..9198
                     /locus_tag="JN701_003074"
     tRNA            9123..9198
                     /locus_tag="JN701_003074"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9156..9158,aa:Thr,seq:cgt)
     gene            complement(9213..10376)
                     /locus_tag="JN701_003075"
     CDS             complement(9213..10376)
                     /locus_tag="JN701_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7365437.1"
                     /translation="MSLFRRGEIWYSSFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2480 a         2573 c         2698 g         2663 t
ORIGIN      
        1 ggggcagtct attttacagc ggcatattgc ctcgttctcg catcatggtt tgcctcaagg
       61 cagtgacgac gcgctacggt atattgcgga acatgacgat gcgctggcgt ttgcgtgtat
      121 taatcgccag atgatcgctt tgcgcataat gcaacaagtt aaggccacgg gaagtccggt
      181 tctggatgtg gcgcataact ttgttagcgc gtgtcaaatc ggcgatcaac agggctggtt
      241 gcatcgtaaa ggtgccacgc cggatgacaa cggtctggtg attattcccg gttcacgtgg
      301 tgattactcc tggattgtac agcctgttgc gaacgaaaaa acgctccact cgctggcgca
      361 tggggcaggg cgtaaatggg ggcgcaccga gtgtaaatgg cgtctggcag cgaaatacac
      421 ggtgacgcaa ctctcgcgga ctgaacttgg cagtcggata atttgtcgcg ataaacaact
      481 catctttgaa gaagcgccac aagcctataa atcggctgaa agcgtggtgc aatgtctggg
      541 gcaggccggt ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc tcaaaaacag
      601 tggagggaaa aaaggatgat cttgctacaa ctctcctcag cgcaggggcc ggaagaatgt
      661 tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag aagctgcccg acaagacgtc
      721 gcggtaacgg tgttggaaac agaaacgggc cgctactctg acacgctgcg ttcagctctg
      781 gtttctctgg atggcgataa cgcatgggca ttaagcgaaa gctggtgtgg cactattcag
      841 tggatttgtc cgagtccgta tcggcctcat catgggcgca aaaactggtt tctgggcatt
      901 gggcgtttta ccgctgatga gcaggagcaa tccgatgcga tccggtatga gacgctgcgt
      961 tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg cgccacgcat
     1021 ttggcatccg gtattagcgt gaaggttcag tcagagcgta gtcagcatgc taacaagcgg
     1081 ctggcacgat tgctgattgc ctggaagctg gagcagcagc aacaggaaaa tagcgcggtg
     1141 ctgaaatcgc agcggcgaat gttccatcac cagattgaac gtggcaaccc gagacggaca
     1201 tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga aggcgattaa
     1261 acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag cagttcagtc
     1321 agcagtgtcc agtaatgacc tacgctttcg atgtgaactt gctcatccgg agagtgtgga
     1381 ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt tttgaacaga
     1441 ccacattcca ggcccgcgtg gataatctgg atgttcggcg tcttgttgaa caggcgctga
     1501 taggtttcac gtaccagatg catcaccgga gaattagcgt ccggctgcca gccaggatat
     1561 gcgcctttcg cttcggtttt cgcgccagcc agtttaccca gcgaatccag catgctcacc
     1621 acgtagtctt taccgctgtc gatcagtgaa cggatcaggc agtgaatttc tacgttattg
     1681 tcagtcatgg tcaccacacc gacgttcagg gaggtttcaa ccacaccttt ggctacatcg
     1741 gagttgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt atcgcgagat
     1801 ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc cagatttttc
     1861 tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag agatttcagt
     1921 gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg gatggcgtta
     1981 cgcagtgtgc cgccgttgaa atcgataagg cgcaggtcca gttcttctgc atgacccgcc
     2041 aggaagcgca ccagcagttt gttggcatta cccaggccaa cgtggatttc accgccggag
     2101 tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc tggaaccgct
     2161 tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc catgtagatt
     2221 tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt
     2281 aagccaaaag caccgtccat accggcttct tcggtcatgg tcagcagcac ttccagcgga
     2341 ccgtgaacca cgttttcgtc agccagaacc gccagtgcag aggccatacc aatgccgtta
     2401 tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata
     2461 ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg
     2521 gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttt acgaatcagg
     2581 atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg
     2641 tattcagcga gttgctcttc atgataggac gggtgcggaa tagaacagat tttggcaaaa
     2701 atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc
     2761 gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag
     2821 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     2881 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     2941 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     3001 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     3061 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     3121 tctggtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     3181 tatttccagc tacgatcacg acaaccagcg agagcttaaa gtgctgaaac gcgcagaagg
     3241 cgatggcgaa ggcttcatag ttattgatga cctggtggat accggtggta ctgctgttgc
     3301 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     3361 tccgctggtt gatgactatg ttgttgatat tccgcaagat acctggatcg aacagccgtg
     3421 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     3481 actgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca gtaagtattc
     3541 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     3601 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     3661 agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     3721 ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt attgtgatga
     3781 gcgatgctga acgtaccgac gatgatttat acgatactgt gattggctac cgtggtggca
     3841 actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc tgtgcggaag
     3901 aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac aacattgccg
     3961 cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta tcaaaccgcg
     4021 cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag tttaccgtac
     4081 ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc ccgttcccga
     4141 cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc ctgtatgaac
     4201 gttattttgc gccgcgcggc attgtgatgc tgactattga tatgccgtcg gtgggctttt
     4261 cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc ttaaaggcgc
     4321 tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc cgtttcggcg
     4381 ctaacgttgc cgtgcgtctg gcatatcttg aatcgccgcg tctgaaagcg gttgcctgtc
     4441 ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag gtgccggaaa
     4501 tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgac gaagcgttac
     4561 gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctgggacgt cgctgcccaa
     4621 cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag gactcacgct
     4681 taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac ccggtgtatc
     4741 ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc ttgtgttaaa
     4801 aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag atagcaatga
     4861 cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca ctaggcccgt
     4921 atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg gctgtatgcg
     4981 tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg ctggtggatg gagctggaag
     5041 cgcaggaatc ccgttttacc tacagctacc agtttggtct gttcgataaa gcaggcgact
     5101 ggaagagtgt tccggtaaaa gatactgaag tggttgaacg actggagcac accctgcgtg
     5161 agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg gaaccggcgg
     5221 atgattttcg tgacgagccg gtgaagttaa cggcgtgagt gaaatgtgcc ggatgcatca
     5281 catccggcaa tatctattaa aactgatacg tcatcccaat cgcgacaata tcatcattat
     5341 taatattcaa tttgttatcg ctatccagtt ggttgatttt ataatcaaca aatgctgaca
     5401 tatttttgtt gaaataatac gtagcaccga cgtcgatata attaaccaga tcttcaccac
     5461 cgatgccctc aatatctttc cctttcgata agacataacc cagcgatgga cgcagaccaa
     5521 agtcaaactg gtattgagcg accgcttcaa agttctgtgt cttattggca aagccgccag
     5581 ttattggcgt cattttgcga gtttcagaat agaaagttgc cagataaata ttattggcat
     5641 cgtatttcag acctgttgcc catgcttctg cacgcttgcc tgtgccacgg ctttgcaggt
     5701 tctgctcgtt ggtgcgatct gagttggtat aggccccact aatggcgaaa tcgctgccgc
     5761 caaagtcata tgtcaatgac gtgccgaagc catcgccgtt ttgcttttta acgtcgcggt
     5821 tttcgttttt cccttgatat tgcagggtta agttcaggcc atcgataacg ccgaagaagt
     5881 cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt cataaagttg tcggtctgcg
     5941 ccgaggagtc gccaccaaat tccggaaaca tatcggtcca ggcttccacg tcatacaacg
     6001 cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt atatttcaac ccggcaaaag
     6061 cgagacgcgt tttttgctgt gcagtatcac tctctgcttt attaccggca aactctgctt
     6121 cccaacgacc ataaccagtc agttgatcgt taatttgtgt ttcgccttta aaaccaaaac
     6181 ggatataact ctggtcgcca tctttactgt cgttatcact catataatgc atggctttaa
     6241 ctttgccata gacatccagt ttattaccgt ctttgttata tatttctgcg gcctgtacgg
     6301 atgcagatgc cacaatgccc atcaccacta atgccagagt gctctttttc attttcattc
     6361 ctgattttaa ttaacgcgcg aatattcagc gggagagccc cgttgaaaac aggaaagttt
     6421 ttaacctgag attgttaaag atatattaca gattaataat attcttaaaa tgtggtaatt
     6481 tattaaatct gtaataaaag cgtaaacaac tgccgctagg cttgctgatc ccgcgcaaca
     6541 aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct aaaacgttcg
     6601 tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag ccagacgctg
     6661 gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa ccgtgcccat
     6721 atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg gattgttatt
     6781 gtgacgtcgg gcgcgatcgc cgccggacgt gagcacttgg gttacccgga actgccagcg
     6841 actatcgcct cgaaacaact gctggcagcg gtagggcaga gtcgactgat tcaactgtgg
     6901 gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac ccgtgctgat
     6961 atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt gctcgataac
     7021 aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat taaagtcggc
     7081 gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa actgttgctg
     7141 ctgaccgatc aaaaaggttt gtataccgct gacccgcgca gcaatccgca ggcagaactg
     7201 attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga cagcgtttca
     7261 ggcctcggaa ctggcggcat gagtaccaaa ttgcaggccg ctgacgtggc ttgccgtgcg
     7321 ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga tgtgatggaa
     7381 ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa ccgtaaacgc
     7441 tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc aactgccgcc
     7501 attctggaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac tggcaatttc
     7561 tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc ccacggtgtc
     7621 agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca agaaattgat
     7681 gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat gattacccgt
     7741 taaggagctg gccgatgctg gaacaaatgg gcattgccgc gaagcaagcc tcgtataaat
     7801 tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc gatgaactgg
     7861 aagcacaaag cgaaatcatt ctcaacgcta acgcccagga tgttgctgac gcgcgagcca
     7921 atggccttag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg ctgaaaggca
     7981 ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc ggtggggcag gtaatcgatg
     8041 gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg ggagttattg
     8101 gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg tgcctgaaaa
     8161 ccggtaatgc ggtgatcctg cgcggtggca aagaaacgtg tcgcactaac gctgcaacgg
     8221 tggcggtgat tcaggacgcc ctgaaatcct gcggcttacc ggcgggtgcc gtgcaggcga
     8281 ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa tacatcgaca
     8341 tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag tcgacaatcc
     8401 cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagt gtagagatcg
     8461 ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca tgtaatacgg
     8521 ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca ttaagcaaac
     8581 aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcactggcg cagttgcagg
     8641 caggccctgc gaaggtggtt gctgttaaag ccgaagagta tgacgatgag tttctgtcat
     8701 tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat attcgtgaac
     8761 acggcacaca acactccgat gcgatcctga cctgcgatat gcgcaacgcc cagcgttttg
     8821 ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt accgacggcg
     8881 gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac gcgcgtggcc
     8941 caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat tacaccattc
     9001 gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg ccattgacgc
     9061 atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca tgtcctttca
     9121 gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc gtaggttcga
     9181 ctcctattat cggcaccatt taaatcaata agttacacat cattagtacg ttccttattt
     9241 cttgactggg acaaatttgg gaccgatggg ttcagaatcg agtctatttg ccgtgcgtgt
     9301 tcggtaaggt gattaggtgc gaggtgagca tatcgacgaa ccatttcgat agactcccag
     9361 cctcccattt cctgtaacac tgataacggg actccggctt gaaccagcca acttgcccag
     9421 gtgtgtctca agtcgtgaaa tctgaaatca tcaatacctg ctcgtctcag cgccgctttc
     9481 caggctgtgt ttgcgtcata ccgcatcttc ctgactgttg gcgctttcgt tccgtctggt
     9541 ttggtacagc tttccttata cacaaatacc caacggtgat gattcccgat ttgttttttc
     9601 aatacgcgac atgcagtatc attcagcgca acgccaattg cgcggtttga tttactctct
     9661 tccgggttta tccatgccac ccggcgctgc atgtctattt gttgccattc aaggttgatg
     9721 atgttcgagc gtcttaagcc tgttgcaagt gcaaattcaa caacagactt taatggctcc
     9781 ggacattcat caatcagcct ttgtgcttca tgaggctcca gccagcggat ccgtttattc
     9841 tttggttgag gcactttaat aattggtgcc ttatccagca ttttccattc acgctctgcg
     9901 gctcttagca gggcctttat aaatgaaaga tgcgtagcct tcgttgcaac ggacgctggt
     9961 tttggcgtgt attctggaac aggtttccct ttttttctgc atgcttctgc cctgagtttc
    10021 cagttttcct catgacgccg gttcgtcatt ttctgcattg ctgaataaat ttttgattca
    10081 gtaatgtctc ttagttgcat tcctgcgaaa tgttgaagcc agaatccgat ccggcttttg
    10141 tcatcgtcca gtgatttttt atgtgctttc tcttcaagcc acctgacaca cgcttcctcg
    10201 aacgttatat caggaatttc accaagtttg ctgacccgcc atgcttcagc ctttagcttg
    10261 tcatggagtt ctgtcgcctg ccttttgtcc tttgttccaa gagactgttt aaatctttta
    10321 ccgttcggca atgtgaaact ggagtaccat atttcacctc tgcggaagag tgacattttc
    10381 tttcctctgt tatgccatca cccgcgctca cctg
//
LOCUS       DADLTD010000155        10372 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLTD010000155 DADLTD010000000
VERSION     DADLTD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.155"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(39..1034)
                     /gene="gapA"
                     /locus_tag="JN701_003076"
     CDS             complement(39..1034)
                     /gene="gapA"
                     /locus_tag="JN701_003076"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7365438.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1376..1789
                     /gene="msrB"
                     /locus_tag="JN701_003077"
     CDS             1376..1789
                     /gene="msrB"
                     /locus_tag="JN701_003077"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY7365439.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1831..2103
                     /locus_tag="JN701_003078"
     CDS             1831..2103
                     /locus_tag="JN701_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY7365440.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="JN701_003079"
     CDS             complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="JN701_003079"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY7365441.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="JN701_003080"
     CDS             complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="JN701_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY7365442.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="JN701_003081"
     CDS             complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="JN701_003081"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY7365443.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6116..6667
                     /locus_tag="JN701_003082"
     CDS             6116..6667
                     /locus_tag="JN701_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY7365444.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6784..7827
                     /gene="selD"
                     /locus_tag="JN701_003083"
     CDS             6784..7827
                     /gene="selD"
                     /locus_tag="JN701_003083"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY7365445.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7832..9793
                     /locus_tag="JN701_003084"
     CDS             7832..9793
                     /locus_tag="JN701_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY7365446.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9920..>10372
                     /locus_tag="JN701_003085"
     CDS             9920..>10372
                     /locus_tag="JN701_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365447.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLITPYRFYHKQWSETLEFWPRKPEPGKDTFQYH"
BASE COUNT         2611 a         2536 c         2736 g         2489 t
ORIGIN      
        1 ccgaagtcgc tctttttaga tcacagtttc atctcaactt atttggagat gtgagcgatc
       61 aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg
      121 aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg
      181 ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct
      241 tcagcagcgg ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga
      301 acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt
      361 ttgccattca gttctggcag tactttacct acagctttag cagcaccggt agaggacggg
      421 atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg
      481 gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag
      541 ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa
      601 acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg
      661 tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca
      721 gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc
      781 catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc
      841 agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc
      901 agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca
      961 gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct
     1021 actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa
     1081 ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc
     1141 tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg aaaccacatg
     1201 gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt
     1261 taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat
     1321 gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc
     1381 taataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac
     1441 gcagaatcat gggacagagc cgccatttac ggggcgttta ctgcataaca agcgtgacgg
     1501 cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tccgaaacca agtatgattc
     1561 cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa
     1621 agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct
     1681 ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa cgttattgtg ttaactctgc
     1741 ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc
     1801 agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg
     1861 acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcctgatggc
     1921 gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac tggtgatgct gtggcaagcc
     1981 cgccataata ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag
     2041 agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc tatgtttaag
     2101 tgaatgagtt atctagtatt taagcctgtt attaagggag ggcaaatata cttgttcgga
     2161 cgtcgtgttt tagctgcaag taggttaaat gcaacactga caagtattat ctggcctaca
     2221 atgtcattga ggaaatgtag gccagagatg aattcaacta atattacccc tgtgtctctt
     2281 cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgtg
     2341 cactgtcctg gggctggata ttcacgccac gacaaccatc ggtaatcacg ttcaccttat
     2401 aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca
     2461 taacgatcaa ttcatcgatt tcatgatcgc gtaaccagtc atcgagagag gttttctgcc
     2521 gacggccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga
     2581 acaccgctgc gatcgctttt tggttcagta acggatgtaa ttgtgcgcct tcactgttct
     2641 gcacacagtg atctggccag aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag
     2701 gctctacacc gtgctgactg gcaaaactgc cgtgattcgc cggatgccag tcctggctgg
     2761 cgataaccgc ttctccgcgc gactggcacc agtcaataag gcggttagca acctccaccg
     2821 tactgtcacc ttccggtacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga
     2881 ccagtaacag ggcgcgatgg ggcattgcag tctccttaat catccggcgt cagttcgccg
     2941 cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg
     3001 taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat aacgccggca
     3061 tgggcgaggg cgttaccggt ggcataacca cccatattca ctttaccgga catacattgt
     3121 gtcaggttga ccaccacaat accgcgatcg ctggcttctt gtaattcctg caggaaggct
     3181 ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt cactggttgg
     3241 cgcagaaaat tgcgcacgac atcagcagaa atccccggat aaatcgtcac tacgccaatt
     3301 ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta
     3361 ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac
     3421 gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg
     3481 aaaaagagcg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc attcagcaga
     3541 ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc
     3601 ggtttaccga gattctcgag catgaacgac agcgcagagg cggtatacgc catcgtgtcg
     3661 gtaccgtgta gaatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca
     3721 atatgctgcc agtcatccgg cgtcatatca gaagaatcca tcaacggcgt atattcatga
     3781 atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt
     3841 agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc
     3901 gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag
     3961 caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg
     4021 gggcgagaca gcgggaagag gattcgtcga ggtaaaaaat aatgtggccg acgatctgtc
     4081 ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt
     4141 ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga
     4201 ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc
     4261 agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg
     4321 ccactgtttc acttttgcga gctctgctgc tttggcaacc gcatcatcga aatccccgag
     4381 actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat
     4441 tttatcaatt tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt
     4501 atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt
     4561 gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc
     4621 cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct
     4681 ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat
     4741 gccgcccatc gatacaacca caggcttacc cgctgcccgg gctgctgcca gttcagcgcg
     4801 aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt
     4861 cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt
     4921 cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc
     4981 accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt
     5041 atcagtctta ctccagccga actctttggt cagagctttt tcgatttcgg cactcgatgc
     5101 cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt
     5161 taaaccctca agcaaccctt gcgcgctagg gaatacctgc tgagcaggga tctgccggtt
     5221 agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc
     5281 gcgggctgcc ggtgacatat catcacgaat aaacggttca acggcagatt tatacgtacc
     5341 cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc
     5401 gttggtagca aaaccgtgca gatcaaccac gccttgcggt gacagccaaa ttttattggc
     5461 gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt
     5521 cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc
     5581 gcctgcgaag tttttcagat ccatcacgat accggtgata ttgtggtcgt ccttcgcctg
     5641 gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc
     5701 aagcagctgg cggcttaatt tactaaaccg ctgagaactg tcgggtttat cgacgatcac
     5761 accggaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact
     5821 gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac
     5881 catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg
     5941 ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat
     6001 gtggttatcg taatgaccca accgacactt gtcagcagga attgcggact gtgctgtaac
     6061 aaaatccact ctcgtgttaa ttttgtgaat gaatatcacg acaggagtta atcaaatgga
     6121 tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac
     6181 gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat
     6241 gcaaccgtgg catttttttg tgattgaagg ggaagggcgc gagcgtttca gcgccgtact
     6301 ggaacagggg gcgattgctg ccggtagtga tgacaaagct atcgacaaag cccgtaatgc
     6361 gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa atcataaagt
     6421 cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc
     6481 cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt
     6541 gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac
     6601 gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta
     6661 tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac
     6721 tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg
     6781 tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt
     6841 aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat
     6901 ccgaatttgc ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga tctgggcaat
     6961 ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat
     7021 tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg
     7081 attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc
     7141 gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt
     7201 cactccatcg atgcgccgga gccgattttt ggtctggcgg taacggggat cgtaccgacc
     7261 gagcgggtga agaaaaacag caccgcacaa gccggatgca aactgttcct gacgaaaccg
     7321 ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgctgaaacc agaacatcag
     7381 ggactagcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc
     7441 gaaggcgtaa aagcgatgac cgacgttacg ggctttggtc tgctgggcca tttgagcgaa
     7501 atgtgtcagg gcgcaggtgt gcaggcgcgc gttgactatg acgcgatccc aaaattgccg
     7561 ggggttgaag agtacattaa gttgggcgca gtgcctggcg gcaccgaacg taactttgcc
     7621 agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatttgct gtgcgatccg
     7681 caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga ggtcaaagct
     7741 acagccgccg agtttggcat tgaactgacg gcaattggcg aactggtgcc agcgcgcggc
     7801 ggtcgtgcca tggttgagat tcgttaattc aatgcggttg tttattgccg aaaaaccgag
     7861 tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat
     7921 cgagtgcggt aatggtcagg tggtgacctg gtgtatcggt cacctgcttg agcaggcgca
     7981 gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattgtccc
     8041 ggaaaagtgg caattacagc cccgaccctc cgtgaccaaa caacttaacg tcatcaaacg
     8101 cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaact
     8161 gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca
     8221 gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgatcg accgtcttcg
     8281 gtccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg
     8341 gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca
     8401 gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga
     8461 tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac
     8521 acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca
     8581 ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag
     8641 tggtcaacca gcaattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct
     8701 gcctttttcg ctttcggcgt tgcagattga agcggcaaaa cgctttggtc tgagcgcgca
     8761 gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg
     8821 ttccgattgt cgctatttgc cagaagaaca ttttgctgga cgccacgcgg tgatgaatgc
     8881 catcagcgtt catgcaccag atctgttgcc gcaaccagtg gtagatccag atatacgcaa
     8941 ccgctgttgg gatgacaaaa aggtcgatgc acaccacgcc atcatcccga ccgcacggag
     9001 ttctgcgatc aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcagta
     9061 cttgatgcag ttctgcccgg atgcggtgtt ccgcaagtgt gttatcgaac tggacattgc
     9121 caaaggcaaa tttgtcgcta aagcgcgttt tcttgctgaa gcaggctggc gcacgctgtt
     9181 aggcagcaaa gagcgcgatg aagaaaacga cggtacgcca ttgcctgtgg tggcgaaagg
     9241 cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca
     9301 ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa
     9361 agatctgaaa aagatccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg
     9421 gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc
     9481 taccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat
     9541 gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga
     9601 ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc
     9661 ggtgcggcag tttcgcggca ttgtggctcc aggcagtggt ggcagtgctg ataagaaaaa
     9721 ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag tcggaagcgg
     9781 ggcgatagcg taagcgagtg aatctttcgt gctattcgag tcatattctg aaatatccag
     9841 cgaatcaaga aaattcgttg gatatttttt tgcatggata aaattatcgc ctctaaagta
     9901 tgtaataaca gggaatgtga tgaaaaaagt tttattacaa aaccatcctg ggagcgagaa
     9961 gtattctttt aatggctggg aaatatttaa tagtaatttt gaacggatga ttaaagaaaa
    10021 taaggccatg ctgctttgta agtgggggtt ttatttaaca tgtgttgtcg ctgtaatgtt
    10081 tgtgttcgca gcgataacat ccaacggttt gaatgaaaga ggcctgatta ccgcgggatg
    10141 ctcttttctt tatctattaa ttatgatggg gcttattgtt cgggccggtt ttaaagcaaa
    10201 aaaagaacaa ctgcattatt atcaggctaa aggtattgag ccgctcagta tcgagaagtt
    10261 acaggcgcta caattgatca caccttatcg attctatcat aagcaatggt ctgaaacgct
    10321 ggagttctgg ccgcgaaagc ctgaacctgg caaagatacc ttccaatatc at
//
LOCUS       DADLTD010000156        10351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLTD010000156 DADLTD010000000
VERSION     DADLTD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.156"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="JN701_003086"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="JN701_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY7365448.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="JN701_003087"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="JN701_003087"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY7365449.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..4073
                     /gene="rbsA"
                     /locus_tag="JN701_003088"
                     /pseudo
     CDS             2603..4073
                     /gene="rbsA"
                     /locus_tag="JN701_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4078..5043
                     /gene="rbsC"
                     /locus_tag="JN701_003089"
     CDS             4078..5043
                     /gene="rbsC"
                     /locus_tag="JN701_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7365450.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5068..5954
                     /gene="rbsB"
                     /locus_tag="JN701_003090"
                     /pseudo
     CDS             5068..5954
                     /gene="rbsB"
                     /locus_tag="JN701_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6080..7009
                     /gene="rbsK"
                     /locus_tag="JN701_003091"
     CDS             6080..7009
                     /gene="rbsK"
                     /locus_tag="JN701_003091"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7365451.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7013..8005
                     /gene="rbsR"
                     /locus_tag="JN701_003092"
     CDS             7013..8005
                     /gene="rbsR"
                     /locus_tag="JN701_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY7365452.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7971..9398)
                     /locus_tag="JN701_003093"
     CDS             complement(7971..9398)
                     /locus_tag="JN701_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY7365453.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9421..10113)
                     /locus_tag="JN701_003094"
     CDS             complement(9421..10113)
                     /locus_tag="JN701_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY7365454.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2523 a         2499 c         2732 g         2597 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaaacat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatcca atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgctgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccgctgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtgct gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggtt ggttgccatt gagcctcggt acggtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgtaccgaag
     1681 acgcgccata tgtccataat gtccgtcggg tacagattga acaactgtcg ctcactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttctcc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaaactt cgggtcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc cagggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtta caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc tgcgttaaat gtctatccgg gccgcgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatgctcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa accatgtatg ccgaagcgga taaattgctg gctaaactta acctgcgctt
     3001 taaaagcgac aagctggtgg gcgatctttc catcggtgac cagcaaatgg ttgaaatcgc
     3061 caaagtgctg agctttgagt cgaaagtcat cattatggat gaaccgaccg atgcgctgac
     3121 cgataccgaa accgaatccc tgttccgcgt catccgcgaa ctaaaatcgc aaggccgcgg
     3181 tattgtctat atctcccacc gcatgaaaga aatcttcgag atttgcgatg ccgttaccgt
     3241 ttttcgtgac gggcaattta ttgctgagcg cgaagtggca tcactgaccg aagattcgct
     3301 gattgagatg atggtgggtc gcaagctgga agatcaatat ccacacctgg acaaagcgcc
     3361 gggagatatc cgcctgaaag tcgataatct ctgcggacct ggcgttaacg atgtctcttt
     3421 tactttacgc aaaggcgaaa ttcttggcgt ctctggtttg atgggcgcag gtcgtaccga
     3481 actgatgaaa gtgctctacg gcgcactacc gcgcaccagc ggttacgtca ccctggatgg
     3541 gcatgaagtc gttacccgtt caccgcagga tggcctggca aacggcattg tgtatatctc
     3601 cgaagacccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagt
     3721 aggctgtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtgttgat ccttgatgaa cctacccgtg
     3841 gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc aaagccgatg
     3901 gcttgagcat cattctggtg tcatcggaga tgccagaagt attaggcatg agcgatcgca
     3961 tcatcgtcat gcatgaaggg catctcagcg gggaatttac tcgtgagcag gccacccagg
     4021 aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag taaaaaaatg
     4081 acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat ggagcagaaa
     4141 tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc gaactttttc
     4201 accatcaata acttattcaa tattctccag caaacctcgg tgaacgccat tatggcggtc
     4261 gggatgacgc tggtgatcct gacttcgggc atcgacttgt ctgtaggttc tctgttggcg
     4321 ctaaccggcg cagttgctgc atctatcgtc ggcattgaag tcaatgcgct ggtggctgtc
     4381 gctgctgctc tcgcgttagg tgccgcaatt ggtgcggtaa ccggggtgat tgtagcgaaa
     4441 ggtcgcgttc aggcgtttat cgctacgctg gttatgatgc ttttactgcg cggcgtgacc
     4501 atggtttata ccaacggtag cccagtgaat accggcttta ctgagaacgc cgatctgttt
     4561 ggctggtttg gtattggtcg tccgctgggc gtaccgacgc cagtctggat catggggatt
     4621 gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta catctacgcg
     4681 ctgggcggca acgaagcggc aacgcgtctt tctggtatca acgtcaataa aatcaaaatc
     4741 atcgtctatt ctctttgtgg tctgctggca tcgctggccg ggaccattga agtggcgcgt
     4801 ctctcctccg cacaacccac ggcggggact ggctatgagc tggatgctat tgctgcggtg
     4861 gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac gttgatcggc
     4921 gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc ctcctattac
     4981 cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa caaaaagcag
     5041 taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac cctggtttcc
     5101 gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac catcgcgctg
     5161 gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc gcagaaagag
     5221 gcggataagc ttggctataa cctggtggtg ctggactccc agaacaaccc ggcgaaagag
     5281 ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat taacccgacc
     5341 gactccgacg cagtaggtaa tgctgtgaag atggctaacc aggcgaacat cccggttatc
     5401 actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc tgataacgta
     5461 ctgggtggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg tgccaaagtt
     5521 atcgagctgc aaggcattgc tggtacatcc gcagcccgtg aacgtggcga aggcttccag
     5581 caggccgttg ctgctcacaa gtttaatgtt cttgccagcc agcagatttt gatcgcacta
     5641 aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc ggatgttcag gctgtattcg
     5701 cgcagaatga tgaaatggcg ctgggcgcgc tgcgcgcact gcaaactgcc ggtaaatcgg
     5761 atgtgatggt cgtcggattt gacggtacac cggatggcga aaaagcggtg aatgatggca
     5821 aactagcagc gactatcgct cagctacccg atcagattgg cgcgaaaggc gtcgaaaccg
     5881 cagataaagt gctgaaaggc gagaaagttc aggctaagta tccggttgat ctgaaactgg
     5941 ttgttaagca gtagttttaa tcaggttgta tgacctgatg gtgacataaa tacgtcatcg
     6001 acagatgaac gtgtaatata aagaaaagca gggcacgcgc caccctaaca cggtggcgca
     6061 ttttatggac atcccgaata tgcaaaacgc aggcagcctc gttgttcttg gcagcattaa
     6121 tgctgaccac attcttaatc ttcaatcttt tcctactcca ggcgaaaccg taaccggtaa
     6181 ccactatcag gttgcatttg gcggcaaagg cgcgaatcag gctgtggctg ctgggcgtag
     6241 cggtgcgaat atcgcgttta ttgcctgtac gggtgatgac agcattggtg agagcgttcg
     6301 ccagcagctc gccactgata acattgatat tactccggtc agcgtgatca aaggcgaatc
     6361 aacaggtgtg gcgctgattt ttgttaatgg cgaaggtgag aatgtcatcg gtattcatgc
     6421 cggcgctaat gctgcccttt ccccggcgct ggtggaagcg caacgtgagc gtattgccaa
     6481 cgcgtcagca ttattaatgc agctggaatc accactcgaa agtgtgatgg cagcggcgaa
     6541 aatcgcccat caaaataaga ctatcgttgc gcttaacccg gctccggctc gcgaacttcc
     6601 tgacgaactg ctggcgctgg tggacattat tacgccaaac gaaacggaag cagaaaagct
     6661 caccggtatt cgtgttgaaa atgatgaaga tgcagcgaag gccgcgcagg tactgcatga
     6721 aaaaggtatc cgtactgtac tgattacttt aggaagtcgt ggtgtatggg ctagcgtgaa
     6781 tggtgaaggt cagcgcgttc ctggattccg ggtgcaggct gtcgatacca ttgctgccgg
     6841 agataccttt aacggcgcgt taatcacggc gttgctggaa gaaaaaccat tgccagaggc
     6901 gattcggttt gcccatgctg cagctgcgat tgccgtaaca cgtaaaggcg cacaaccttc
     6961 cgtaccgtgg cgtgaagaga tcgacgcatt tttagacagg cagaggtgac gcttggctac
     7021 aatgaaagat gttgcccgcc tggcgggcgt ttctacctca acagtttctc acgttatcaa
     7081 taaagatcgc ttcgtcagtg aagcgattac cgccaaagtt gaagcggcga ttaaagaact
     7141 caattacgcg ccatcagccc tggcgcgtag cctcaaactc aatcaaacac ataccattgg
     7201 catgttgatc actgccagta ccaatccttt ctattcagaa ctggtgcgtg gcgttgaacg
     7261 cagctgcttc gaacgcggtt atagtctcgt cctttgcaat accgaaggcg atgaacagcg
     7321 gatgaatcgc aatctggaaa cgctgatgca aaaacgcgtt gatggcttgc tgttactgtg
     7381 caccgaaacg catcaacctt cgcgtgaaat catgcaacgt tatccgacag tgcctactgt
     7441 gatgatggac tgggctccgt tcgatggcga cagcgatctt attcaggata actcgttgct
     7501 gggcggagac ttagcaacgc aatatctgat cgataaaggt catacccgta tcgcctgtat
     7561 taccggtccg ctggataaaa ctccggcgcg cctgcggttg gaaggttatc gggcggcgat
     7621 gaaacgtgcg ggtctcaaca ttcctgatgg ctatgaagtc actggtgatt ttgaatttaa
     7681 cggcgggttt gacgccatgc gccaactgtt atcacatccg ctgcgtcctc aggccgtctt
     7741 taccggaaat gacgctatgg ctgttggcgt ttaccaggcg ttatatcagg cagagttaca
     7801 ggttccgcag gatatcgcgg tgattggcta tgacgatatc gaactggcaa gctttatgac
     7861 gccaccatta accactatcc accaaccgaa agatgaactg ggggagctgg cgattgatgt
     7921 actcatccat cggataaccc agccgaccct tcagcaacaa cgattacaac ttactccgat
     7981 tctgatggaa cgcggttcgg cttagattta cgctgtcttt tgatcaaatt attaccatcg
     8041 gttgttttca gaagcatgaa cattgctgct gaagcaacag taataatgcc catcgtgata
     8101 aacgtatagt ggaattgttc gacagtcgtt gtgccttcca tcccttcata aacgcgaagg
     8161 acggccgcac ttacagcaac gcctaaacta atcgacagtt gctgcgtgac cgccagaaca
     8221 ctgttaccgc tgctggcgtt gtcatcggtc agatcggcaa gtgtgatggt attcatcgcg
     8281 gtaaattgcg tcgacatagc catccctaat ataaacaacg gcaagatcag catccatatc
     8341 gccattgccg gtgattgcaa agagaactga gcgatcatta gcccaataat caccgtgatc
     8401 cccactaacg tatggcgata gcccagacga cgtaagactt gggtaaccat cgattttgca
     8461 ataatggaac ctaacgctgt cggtgccatc atacagccgg caataaacgc ctgataacca
     8521 aatcctacct gtaacatcaa tggcataagg aacggtacac taccggtccc cagacgggtt
     8581 gcaatattgc ctacgatacc gatcgagaaa gtgcgggttt taaataaatc taatgaaatt
     8641 aatgggtttg gcgtgtgtcg cgcatggaga atatagagaa gcagtaaccc gatgctggtg
     8701 acaattaccg tcaaggcaat ccagctggcg acaatctttt ccccgaatag ctctattccg
     8761 cttgagaaga gaacaaggct gaggccaaac agcaaaaagc cagtgatatc gaatctgcgt
     8821 cgtgcggtgg tgaaattggg catatgtttg cgcgcgtaaa gaaggcccgc aatacctatg
     8881 gggatattga ttaaaaatat ccagtgccag gttgcccagg tgaccagcac gccgccaaga
     8941 acggggccta aaattggccc caccagaccc ggcatggcga caaaattcaa tacaggaaga
     9001 agttcattac gaggataagc acgcagtaag gccagccgag caacaggcat catcattgcg
     9061 ccgcctatcc cctgaataac ccggaagaca accagctgtg gtagcgaatt agaaagtgcg
     9121 caggccagag aacccaacgt gaacagactc acggcaaggg taaaaatgcg acgcgtaccg
     9181 aagcgatcgg ctagccatcc gcttaccgga ataaacatcg ccaccgtcag cgtataactg
     9241 atgatggctg attgcatcgc gagaggagaa cgattaaggc tatgagcgat tgcgggtaag
     9301 gcggtattca gaatagtggc atcaagtgcc tgcatgaaga aggccatcgc cgcgatccac
     9361 ggcaaacccg ccatactacg cttcttttta tcgctcattc aatgtcctgt tatcgggtta
     9421 tcacttatca ggtgagcgta gcagcgcctg acaagtttta aatgccgcgt cgccatcgct
     9481 ttggataatc gcatcgacaa tcgcctggtg cagatccagc tttatcactg tgtcgctggt
     9541 aattgacgtg aagtaagtgt gataaaccga atggaatagc gaggcgaatg aggtcaaaaa
     9601 cggattggcg ctcatttcat agatatgctc atgccaggcc atatcgactt cgatccagcg
     9661 ttcacggcga aagttctttt ttaatgccgc catttcggcc attaacgtat tgagatgcgc
     9721 cttctgttcc gcggtgccaa ccgttgctgc cagtaggcag gcttgcggct ccagacagat
     9781 acgcataacc agaaagtgat cgatgacctg atgaaagttc tcttctgtca tccaccaggt
     9841 aagcaattcc tgatcaagaa aattccagtt tgattgtggc atgacccggg taccaattcg
     9901 cggtcgcggt aaaaccatcc cttttgccgt taacgttttg accgcttcgc gtaccgctgt
     9961 acgactcact ccaaattgct cgcccagctc aatctcacca ggcaaaatgg tgccgggttc
    10021 atattcacct tttaagatgc gttgcgccag cttctcagcc agaacatacg aaaggttttt
    10081 ctgtgcagct aactgttgtg cgcttaaagg cattactaat cttccttttt ctttttattc
    10141 ctccttagta tgccactagg aagtgtgatt acggttgcaa aaacggcaaa ttgcttgttt
    10201 tatggcacat taacggggct tttgctgaaa aagtgcgcgg tcagaaaatt attttaaatt
    10261 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
    10321 aaacacgccg ccgggtcagc ggggttctcc t
//
LOCUS       DADLTD010000157        10330 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLTD010000157 DADLTD010000000
VERSION     DADLTD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.157"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            121..987
                     /gene="folD"
                     /locus_tag="JN701_003095"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="JN701_003095"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY7365455.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="JN701_003096"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="JN701_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY7365456.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="JN701_003097"
     CDS             1309..1830
                     /locus_tag="JN701_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7365457.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="JN701_003098"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="JN701_003098"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY7365458.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="JN701_003099"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="JN701_003099"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY7365459.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="JN701_003100"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="JN701_003100"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY7365460.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="JN701_003101"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="JN701_003101"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY7365461.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="JN701_003102"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="JN701_003102"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY7365462.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="JN701_003103"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="JN701_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="JN701_003104"
     CDS             complement(7360..8175)
                     /locus_tag="JN701_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAY7365463.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="JN701_003105"
     CDS             complement(8186..9445)
                     /locus_tag="JN701_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAY7365464.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..10279)
                     /gene="fdrA"
                     /locus_tag="JN701_003106"
                     /pseudo
     CDS             complement(9455..10279)
                     /gene="fdrA"
                     /locus_tag="JN701_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314544.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2272 a         2936 c         2774 g         2348 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agctacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgatttcac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagtccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgcgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgcggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc atcggatggg
     9961 gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga
    10021 gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgtcca gcaagtaatc
    10081 ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc gcaaataaat ccgctgctga
    10141 caggcgctat tgcgttacgt cgcgccgtga cgcgtgaaag caggcaagcc aggcgtgcgg
    10201 cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac
    10261 ctaaaaacag cgccaccatc ggtttgccgg ttgctttcat ggcattaaca attttcagac
    10321 gcacagcttc
//
LOCUS       DADLTD010000158        10205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.158, whole genome shotgun sequence.
ACCESSION   DADLTD010000158 DADLTD010000000
VERSION     DADLTD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.158"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="JN701_003107"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="JN701_003107"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY7365465.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGHFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="JN701_003108"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="JN701_003108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY7365466.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="JN701_003109"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="JN701_003109"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7365467.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="JN701_003110"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="JN701_003110"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY7365468.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="JN701_003111"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="JN701_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY7365469.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="JN701_003112"
                     /pseudo
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="JN701_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            5724..5885
                     /locus_tag="JN701_003113"
     CDS             5724..5885
                     /locus_tag="JN701_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY7365470.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="JN701_003114"
     CDS             6007..7080
                     /locus_tag="JN701_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7365471.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="JN701_003115"
     CDS             7077..7859
                     /locus_tag="JN701_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7365472.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8073..8936)
                     /gene="yjjW"
                     /locus_tag="JN701_003116"
     CDS             complement(8073..8936)
                     /gene="yjjW"
                     /locus_tag="JN701_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY7365473.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8908..>10205)
                     /locus_tag="JN701_003117"
     CDS             complement(8908..>10205)
                     /locus_tag="JN701_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY7365474.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
BASE COUNT         2409 a         2665 c         2750 g         2381 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt tacgccgcat
      241 ccggcaaggc ttgcggcggg atacttaacc ttttttcgcc tggcgggtca taatggcgcg
      301 atataccttg aaatgcccgg tttgcgcaat cacttcatgg aagccaaatg tttcatccag
      361 cacgtccgga taaggcagga aggcgttcgc tacaattcgc agctcgccgc cactattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc gtatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa aactttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgtg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctgaaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcttc
     2101 aaacgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gtttcgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgctaaaatc
     2581 ggcatcatca ccaacggttt tagcgccttg cagcaagtgc gtctggaacg gacgggcctg
     2641 cgtgattact ttgatttgct ggtgatttcc gaagaagttg gcattgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaactggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacttgc
     2821 tggctgaatg cgcaccatct cgagcaacca gaaggcatcg cgcccacctg gaccgtttca
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggacgc cggtaaaact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgctgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgcta gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac agcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctttatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga actgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggcccct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaatatg gacccgaaac atcgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 aacgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cacgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcggccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgag caaaaagtcg tgaccctgag cggtttcgtt gaaagctagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctcggtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtagat tctcaggcac
     5521 aaagtgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtt tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgtaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggttg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgattg acgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgacacc cactgccatt ttgatttcct gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgt gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattgggctt gcatccaggt atgttggaaa aacatagcga tgtgtctctt gagcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcagt gggggaggtc ggtctggatc
     7381 tcttcggcga cgatccgcaa cttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc tgcattcacg gcgcacacac gacaaactgg
     7501 cgatgcatct taaacgccac gatttatcgc gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagt
     7861 ttacccttcg taggcagtat ccggcagtcg gcgcataatg cctgatgcga cgcttgtcgc
     7921 gtcttatcat gcctacaggt ttgtgccgaa ccgtaggccg gataaggcgt tcacgccgca
     7981 tccggcagtt ggcgcacaat gcctgatgcg acgcttgtcg cgtcttatca tgcctacaag
     8041 tctgtgccga accgtaggcc gtatccggca tgtcacaaat agagcgccgg aaatatcaac
     8101 cggctcaccc cgcgcacctt taacgcatca gccaacggct caacgtcttc cggcgtggcg
     8161 ctcgcccagc tttgcgcctc gccatacacg ccgtgggcat gaaacgcatt caggcgaacc
     8221 ggaacatcgc caagtctctt gataaacgtc gccagctcat cgatgtgatg caaataatcc
     8281 acctggtctg gaattaccag caaacgcagt tccgccagct tgccgcgctc tgcaagcaaa
     8341 cagatgctgc gcttaatctg ctgattatcg cgtccggtta agtgttgatg acattcgctc
     8401 ccccacgctt tgagatcgag cattgcgccg tcacacaccg ggagtaattt ttcccagccg
     8461 gtttcgctca acatgccgtt actgtccacc aggcaggtga ggtggcgcag ttgcggatcg
     8521 tttttgatag cagtaaacag cgccactaca aacggcagtt gggtcgtggc ttcgccgccg
     8581 cttaccgtta tcccttcaat aaacagcacc gctttgcgga catggctaag cacttcgtcc
     8641 acgctcatgg attgcgccat tggcgtggca tgttgcggac acatcttcag gcaggtatca
     8701 cactgctcgc agaccacagc gttccacagc actttgccgt caacaatctg caacgcctga
     8761 tgcggacact gtggcacgca ctctccgcaa tcattgcagc gtcccatcgt ccacggattg
     8821 tgacagtttt tgcagcgcag attgcagccc tgcagaaaca gagccagacg actgcctggc
     8881 ccgtcaacgc aggagaaggg gataatctta ctgactaaag cgcatctgct gttcatggct
     8941 tatcacgcgc ggctggcgtt ccagaatacg agtgttgcgt gtggcttctt cgcccagcca
     9001 ggtggtgttg gtgcgtgaac cttcggcgcg atatttttct aaatccgaca aacgcaccat
     9061 ataaccggta acgcgaacca gatcgttacc gctgacattg gcggtaaatt cacgcattcc
     9121 ggctttaaag gcaccgaggc aaagctgtac cagcgcctgc ggattacgtt tgatggtttc
     9181 gtcgagcgtc agaatgtcgc tgatgccgga ataataataa gcatgatgcg gggcgacagt
     9241 ttgcagatgg atgatcggat ctggctcatc gccatacggc aaacgcgcgc ccggcgtggt
     9301 gccgatatcg gaactgatcc ccgactgtgc gtgtaacatg gcgcgttttt gccagccata
     9361 tttcacgggg gtattggcga caaactccgc cagttgcgcg ctgatgcgat aacccacttc
     9421 atttgcggcg gcttctttac cgtagcgcgc ggcaatccct tctttctcac acagcaagtt
     9481 aaccgcttcc gccagcccat acatgccaaa cattggcaca aaacgttcag ggttaatcag
     9541 cccttctttc accaggaagc tattctcaaa gaagtgtgat tgttgataga ggaattcaca
     9601 ccgcgcatcg atgatggcga tctgctgctg gcagtagtgc ggtagagtgc gcgtaaagaa
     9661 gtcatccagc gattcgctgc gctcggcaat ggctttcagg ttaaggcgta ccagcgtgct
     9721 gccaccaccc gccagcggca gtgagttgta acagctcaca atcccgtagc ccccttttgt
     9781 gaaaatttta tcatgcaccg gaccgttggc gatgtgcggt ttgctacatt cacagatgtt
     9841 cttcgccact tccagcagca ggtcatcagg ggggatttca ggatcgtaga taaaggtcag
     9901 gttcggtgaa acctgcttca actctgcgtc tgcacgtaag atcgcgcggg taatgggcga
     9961 atcagacggg ccgatattgg cgtgcataaa ggcgtctggc agggttccgt cgaggtaacg
    10021 ccagaaacgt tttattcgaa catcgatctc gtcttgtgtt agaattctaa catacggttg
    10081 caacaacgca tccagttgcc ctaggtagac cggcatcgat gtgaccgacg gtacgtggtg
    10141 gtaaagaatg gtcagcagag agagtgcgtc atcaagatct ttcgcgcctt ccagctccag
    10201 ccatt
//
LOCUS       DADLTD010000159        10115 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLTD010000159 DADLTD010000000
VERSION     DADLTD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.159"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            203..355
                     /gene="hokA"
                     /locus_tag="JN701_003118"
     CDS             203..355
                     /gene="hokA"
                     /locus_tag="JN701_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY7365475.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(543..755)
                     /gene="cspA"
                     /locus_tag="JN701_003119"
     CDS             complement(543..755)
                     /gene="cspA"
                     /locus_tag="JN701_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY7365476.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1036..1326)
                     /locus_tag="JN701_003120"
     CDS             complement(1036..1326)
                     /locus_tag="JN701_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY7365477.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1759..2469
                     /locus_tag="JN701_003121"
     CDS             1759..2469
                     /locus_tag="JN701_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY7365478.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2519..3493)
                     /gene="ghrB"
                     /locus_tag="JN701_003122"
     CDS             complement(2519..3493)
                     /gene="ghrB"
                     /locus_tag="JN701_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY7365479.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3597..4256)
                     /locus_tag="JN701_003123"
     CDS             complement(3597..4256)
                     /locus_tag="JN701_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY7365480.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4409..6742
                     /locus_tag="JN701_003124"
     CDS             4409..6742
                     /locus_tag="JN701_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7365481.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAESSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6711..7151)
                     /locus_tag="JN701_003125"
     CDS             complement(6711..7151)
                     /locus_tag="JN701_003125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7365482.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7148..7711)
                     /gene="tag"
                     /locus_tag="JN701_003126"
     CDS             complement(7148..7711)
                     /gene="tag"
                     /locus_tag="JN701_003126"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY7365483.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7869..8567
                     /locus_tag="JN701_003127"
     CDS             7869..8567
                     /locus_tag="JN701_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7365484.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8795..9997
                     /locus_tag="JN701_003128"
     CDS             8795..9997
                     /locus_tag="JN701_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365485.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2426 a         2436 c         2592 g         2661 t
ORIGIN      
        1 ttttaggatt atatcaagga gaagaaacaa acttattaag ctagaatagc tacgggtgct
       61 tgagactgtt tgtctcaggt attcaccgaa aggcagacag agaaaagccc cacctgacta
      121 taaatcaaag tgaggctacc ctatgcctga acaccataag gttagcctct tactcgttgg
      181 aaatcaacac agggggctgg gaatgccgca aaaatacgga ttactttcat taatagtgat
      241 ttgtttcacg cttttatttt tcacctggat ggtaagagat tcactgtgtg aattgcatat
      301 taaacagggg cgttatgagc tggcggcatt tttagcctgc aatttaaaag agtaagagtc
      361 ttcggcggga aattattccc gcctttctta cggcgttgcg cattctcatt gcacccacat
      421 ttattcttca caaaaataat aatagatttt actacgcgat cgattattta tttcctgaaa
      481 aaaaataaaa aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga
      541 gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag
      601 gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt
      661 acgaacacat ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg
      721 aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt
      781 gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta
      841 cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct
      901 gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc gggtgatgca acaattattt
      961 ttcatattta tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat
     1021 aaagtggata aaagtctatt ccatcaactg cttacttaat gccgggttgg cttgaatcaa
     1081 acgcatcaat tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac
     1141 catggcgact gatatgccca aaacccgggc gaaatcatcg atttttaacc ctgtcccttt
     1201 tcgtaactgc tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt
     1261 ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata
     1321 ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga agctcattaa
     1381 gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc
     1441 gattcagcca gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat
     1501 atctcttacc actcctgttt tgtaaagatt tcgaaaatct aaagttctgc gaaggcgtaa
     1561 atctcgggct acaaaaaagc aacggcaata tcatttttta acaatgaaac aacaaattaa
     1621 ccttgtttca ttttctccat gattttacca ggaagattac gtagaaaggg tatcctgcaa
     1681 gcctgtcctg gactatcctt gtcacgtcag acacgcgtgt gtcgttgtgc gctttttttg
     1741 ggtgaaagga gtaagaaaat ggcgacagga aagtcctgct ctcgctggtt tgcgcctctt
     1801 gcggcgttat taatggtagt tagcctgagt gggtgttttg ataaagaagg cgatcagcgt
     1861 aaagcgttta tcgacttcct gcagaataca gtgatgcgta gcggtgaacg tctaccaacc
     1921 ctgactgccg atcagaaaaa acagtttggt ccttttgtct ctgattacgc gattctgtat
     1981 ggttattctc agcaggtgaa tcaggcgatg gattccggtc tgcgcccggt cgtagacagc
     2041 gttaacgcaa ttcgcgtacc gcaggattac gttacgcaaa gcggtccgct gcgtgaaatg
     2101 aacggttcgc tgggtgtgct ggcgcaacaa ctgcaaaatg cgaagttgca ggccgatgct
     2161 gcgcactctg cgctaaaaca gaccgatgat ttgaaaccgg tctttgatca ggcattcacc
     2221 aaagtggtaa cgacgccagc tgatgcattg caaccgttga ttccggttgc acaaactttc
     2281 acgcaacaac tggtgatggt tggagactac attgctcaac aaggtactca ggtgagcttt
     2341 gtagcaaatg gtatccagtt cccgacttcg cagcaggcaa gtgaatataa caaactgatt
     2401 gcgccgttac cggcacaaca tcaggcgttt aatcaggcct ggactacagc agttactgca
     2461 acccaataaa gagtaaaagc ccgagcgatt atcgggcttt acttcacgca gtcgcggctt
     2521 agtccgcgac gtgcggattc acacagttct tctcaacctt tccttgtaac gcatcaatca
     2581 aattatccac ggcacaggcg gccatgccat aacgcgtctc atgggtggca gatccaatat
     2641 gcggtactgc gacgacgttg gccattgaga gcaacggcga atctacggac agtggctctt
     2701 gttcgaagac atccagcccg gcggcgtgaa tttcgccttt ctgcaatgct gcgatcagtg
     2761 cattttcgtc aaccaccggg ccacgtccgg cattaatgaa aatggcggag ggtttcattt
     2821 tggcgaattg ttctgcgcca aacagatgat gcgtctcatc agttaacggc aggatcaggc
     2881 aaacgaaatc tgactcttgt aacagagtat ccaaatcgca gtagcgggcg ttgaagcgtt
     2941 cttctgcttc tttatggtgg cggcgcgcgt tatagaggat gggcatgttg aagccaaagt
     3001 gcgcacgttg tgccagcgcc atgccgatcc gtcccatccc gacaatgccc agtgttttat
     3061 ggtgaacgtc agtgccgtac cagtccgggc ctatgctcgc ggtccattcg cctgctttta
     3121 cccgttctgc tacctccaca acccgacgag cggtagacaa caccagcgcc atcagcgtat
     3181 cggcgacggt ttctgttaat acggttggcg tgtgcatcag cagaattttt cgggcggtaa
     3241 gcgcatcgac atcaaaattg tcatagccga cggagatcgt tgatgtggca cgcagtttcg
     3301 gcattttttc cagcaatgcg gcatctacat tctcgtttga acccagtaaa ccttcagctt
     3361 cggcaaaaat tgctgcattt tgttcgacgg tttgtgggct gaggtttgcc acctggtgaa
     3421 cggtgaaatg ctcttgcagg cgttgcagta aatcatcagg taaggctttg tagaggataa
     3481 cggacggctt catgcttctc tccattcact gataaggcgc tcaccttagc ctgaaatatg
     3541 atgaaggaaa actgaagaat gccaggtgat ttctgcatca cctggcatga aagggattac
     3601 agcgggctta aggtaatttc tacacggcgg ttttgcgcct taccttctgc ggtgctgttg
     3661 ctggcgattg ggttagccgg gccaaggccc tgagtacgga tgcggctggc gtccacgccc
     3721 tgggtgatca acgcgctggc aacggaatcc gcacgctgct gggagagacg catgttcagg
     3781 tcgtgacccc ccgtgctgtc ggtataacca atcacgttaa ccgccgtttt cggatactct
     3841 ttcagcacca ttgccacgcc ggtcagggtg ttagcgcctg ccggtttcag ggtcgcgctg
     3901 ctgctgtcga aggtcacatt gttcggcata ttgaggataa tgttatcccc gctgcgggtt
     3961 acgctaacac cagtgccgcg cattttgtca cgcagcttcg cttcctgcac atccatgtaa
     4021 taacccacgc cgccgcccag agctgcgcct gctgctgcgc caatcagcgc gcctttaccg
     4081 cgatctttct tcgaagaaga gagcgcacca atacccgcgc ccacgagaga gtccagacct
     4141 gcgccgatag cagatttgcc tgcttcgcgt tcgccggtgt aagggttagt tgtgcagcca
     4201 gataccgcca gagcgccact cactacggcg gcaataagat aaacacgttt cttcattgtt
     4261 aatccttaat aaccttttta ttctttgcca cgggttccgt ggcgggagat tatgccgcgt
     4321 gaacatgaag attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa
     4381 tacatctaac ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct
     4441 gactgccgcc cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc
     4501 gcgtggcgcg ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt
     4561 tcacagcaat acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga
     4621 aaacccgcaa ggcattcgtg ggcaggatga atttgttcac gtgagttggg atgaggcgct
     4681 ggatcttatt caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt
     4741 tgctggttcc tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca
     4801 acgctatatg gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggtgc
     4861 ggcacaggcg atcatgccgt atgtcgtggg tggcagcgaa gtttatcaac agcaaaccag
     4921 ttggccgctg gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa
     4981 tacgctgaaa attgcgtgga atgcatctga tgagcagggg ctttcttact tttccgcact
     5041 gcgtgacagc gggaaaaagc tgatctgtat tgatccaatg cgatcggaaa ccgtcgattt
     5101 ctttggcgat aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct
     5161 ggggatcgcc catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg
     5221 caccacaggt tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagccaa
     5281 aaccgccgaa tgggcggcag ggatttgtgg ggttcacgca gcgaaaattc gcgagttggc
     5341 ggctcttttc caccaaaaca cgaccatgct gatggctggc tggggaatgc agcgccaaca
     5401 gcttggtgag caaaaacact ggatgatcgt cacgctggcg gcaatgttgg ggcaaatcgg
     5461 cacacccggc ggcggttttg gtctttctta tcattttgcc aatggtggta atcccacgcg
     5521 gtgttctgcg gtgctctctt ccatgcaggg tagcttgccg ggtggcagcg atgcggtaga
     5581 taaaatccct gttgcccgta ttgttgaagc actggaaaac cctggctgcg cataccaaca
     5641 caatggcatg aatcgcctct tcccggatat tcgctttatc tggtgggcgg gtggcgccaa
     5701 cttcacccat catcaggaca ctaatcgcct gatacgagct tggcaaaaac cggagctggt
     5761 agttatctcc gaatgcttct ggacggcggc ggcaaaacac gcggatatcg ttctgcctgc
     5821 gactacctct tttgagcgta atgatctcac catgaccggc gattacagta atcagcatct
     5881 ggtgccgatg aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt
     5941 tgccgagtca agtgaacgct gggagaaggg cggttatgca cgttttacgg aaggaaaaag
     6001 tgagttgcaa tggctggaaa cgttttataa cgttgcccga cagcgcgggg caagccagca
     6061 ggttgaattg ccgccatttg ctgagttctg gcaagccaat cagttaattg agatgccgga
     6121 aaacccggac agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgcaggcgca
     6181 tccattaaaa accgccagcg gtaagattga aatcttctcg cagcgtattg ccgattacgc
     6241 ttatccggat tgcccggggc atccaatgtg gctggagccg gacgaatggc agggcaatgc
     6301 cgaaccggaa cagttacagg tactttctgc tcatccggcg catcgtctgc acagccagct
     6361 gaattacagt tctctgcgcg aattgtatgc ggtggcaaat cgtgagcctg tcaccattca
     6421 tcctgacgat gccctggcgc gcggcattca agatggcgat actgttcggc tgtggaacgc
     6481 acgcgggcaa attcttgccg gagtggtcat tagcgaggga attaaacctg gcgtgatttg
     6541 tattcacgaa ggggcatggc cggatttgga tttaaccgct gacggtattt gtaaaaacgg
     6601 cgcagtcaac gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg
     6661 taatacggcg ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt
     6721 tgaaccaccg gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa
     6781 tcgacaatgt gaaaaccctg cgcctggtaa aaatttatcg ccggttgatt tttttgataa
     6841 acctccagca tcaggtgggg atggcgctgc tgcacatatt gcatcagcgc cttaccaata
     6901 ccgcgcctga cggccttcgg tgcgacaaac atcgctgcca gaaatcggcc ttccataatg
     6961 ctgacaaaac cgagaagctt actgtcttct tcccagaccc agttttgcgc attggcaaga
     7021 tacgcatccc gcaccagcgg aatgcaatca cgccagtaat tcgcttttat aaagggatgt
     7081 ccccaggttg tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct
     7141 tcccgaatca tggtttattt cccggatagc aacagcagcc aaccacatga tcattcacca
     7201 gcccacatgc ctgcataaag gagtaacaga ttgtggtacc gacaaactta aaaccacgtt
     7261 ttttcagtgc tttagatagg gcgtcggagg cgggcgtaga tgtggggatt tcgctcaacg
     7321 ttgtggcttg tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcggcaaacg
     7381 gttcgccgtt ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt
     7441 tcccgcgatg gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca
     7501 ttgctgcgac cttcaccgga tcgaactgat gaaagcaggc gcgatagttt tcgcgttttt
     7561 tgaggacggt gatccacgat aacccagcct gctgcccttc aaggcagatc atttcgaaca
     7621 gttttttact gtcagtttca ggcacgcccc actcattatc atggtaggca atataaagcg
     7681 ggtcctgact cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa
     7741 aaatttcagc cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac
     7801 tatgcccgat atccttcata caatgacaat aatattgccg aattcggctc ttccctggag
     7861 tcgtgttaat gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg
     7921 tggtaatcag cgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg
     7981 atacgcaacc cggacattcc acagagcgtt acacctggga tagtgatcat caacctgatt
     8041 acaacgatat tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca
     8101 atctggcgga agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc
     8161 ctttatatga acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca
     8221 cctcgatgta taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg
     8281 ccagcgtgag tagcttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg
     8341 cgcaaatcag ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga
     8401 gccggatgac ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata
     8461 tgttgctcaa tcaaaatatt gccgcctatg ccgcgctaac tcaggcagaa aataccacta
     8521 ataatagcga ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca
     8581 attgaaacca ttgaataaca atagttgtgg tgatagtggg tgctaactta caaataatga
     8641 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8701 tggcatttca tgccgttttt ccccaggcat aaaatgcact tcgttatggt tgtcggcaga
     8761 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8821 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     8881 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     8941 tttctctttc ggcttgttaa gtctggggct ggcaatttca tcttctgttg cggggaaatt
     9001 acaggaacgt tttggcgtta aacgcgtcac tatggcttcc ggcattttgc taggattagg
     9061 cttcttcctg acagcgcatt ctgacaacct gatgatgctg tggttaagcg ccggtgtgct
     9121 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9181 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc ttggtagcct
     9241 gggtttcaaa tttatcgaca cgcagctgct ggaaacggtg ggtctggaaa aaacatttgt
     9301 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9361 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9421 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9481 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9541 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
     9601 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
     9661 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
     9721 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
     9781 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
     9841 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
     9901 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
     9961 gccagagcag aaaatgttgc gtgaggcgca tatttagaaa tatgttgcta ctgaaataaa
    10021 tacgaggagg gagacttcaa agtctccctt ttttatggac gcatattaaa agaacaataa
    10081 tattcatacc acaaatatat tttatttata tatta
//
LOCUS       DADLTD010000160         9999 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLTD010000160 DADLTD010000000
VERSION     DADLTD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.160"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(264..1607)
                     /gene="gdhA"
                     /locus_tag="JN701_003129"
     CDS             complement(264..1607)
                     /gene="gdhA"
                     /locus_tag="JN701_003129"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY7365486.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1843..2115
                     /locus_tag="JN701_003130"
     CDS             1843..2115
                     /locus_tag="JN701_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY7365487.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2081..2488)
                     /gene="nudG"
                     /locus_tag="JN701_003131"
     CDS             complement(2081..2488)
                     /gene="nudG"
                     /locus_tag="JN701_003131"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY7365488.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2575..3186
                     /locus_tag="JN701_003132"
     CDS             2575..3186
                     /locus_tag="JN701_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7365489.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3195..4502)
                     /locus_tag="JN701_003133"
     CDS             complement(3195..4502)
                     /locus_tag="JN701_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY7365490.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4569..5222)
                     /locus_tag="JN701_003134"
     CDS             complement(4569..5222)
                     /locus_tag="JN701_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7365491.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5222..6757)
                     /locus_tag="JN701_003135"
     CDS             complement(5222..6757)
                     /locus_tag="JN701_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7365492.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCTLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(6730..7896)
                     /locus_tag="JN701_003136"
     CDS             complement(6730..7896)
                     /locus_tag="JN701_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7365493.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7906..8454)
                     /locus_tag="JN701_003137"
     CDS             complement(7906..8454)
                     /locus_tag="JN701_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY7365494.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8454..9161)
                     /locus_tag="JN701_003138"
     CDS             complement(8454..9161)
                     /locus_tag="JN701_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7365495.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9176..9853)
                     /gene="ydjY"
                     /locus_tag="JN701_003139"
                     /pseudo
     CDS             complement(9176..9853)
                     /gene="ydjY"
                     /locus_tag="JN701_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            complement(9858..>9999)
                     /locus_tag="JN701_003140"
     CDS             complement(9858..>9999)
                     /locus_tag="JN701_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7365496.1"
                     /translation="RFILQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKN
                     EG"
BASE COUNT         2384 a         2726 c         2584 g         2305 t
ORIGIN      
        1 atctgctgct atcgcagaaa gttaacggat gctgaacttg aagcgtgtta tcgttacgac
       61 aagcagtttt gggagcacta cagtaagaaa tgccgttggc ccataagaaa tgttccctgg
      121 ggagcatcat ccgttaaata ctcataaacg cctgaaattt tgccgggggc gctgcgcttg
      181 cccggcctac attagagcgt aactgcaatg aattgtgccc atttgtaggc ctgataagcg
      241 tagcgccatc aggcatttac aacttaaatc acaccctgcg ccagcatcgc atcggcaacc
      301 ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg tttgctcacc ttcaccacca
      361 tgctcaacac aggcatggtg gatatccagc atgatgtgat gcaaacgtgc gtcaactttc
      421 tcggctttcc agcccaggcg cgcagcgttt tgcgccattt caaggcctga cgttgctaca
      481 ccgccagcat tagccgcttt acccggtgca aacagtacgc ctgcctgctg gaacagttca
      541 gtcgcttcga tggtggtcgg catatttgcc ccttcggcga cggctttaac gccattggcg
      601 ataagctgat gcgcggcgcc aacatccagt tcgttctggg tggcgcaagg cagggcgata
      661 tcaaccggta cagaccacgg ctgttggcct tcgagataga ccagaccaaa ttctttggcg
      721 taatctgcca ctcgaccatc gcggctggct ttgatttcga taagacgtgc cagtttctct
      781 ttcgtgaatc cgctttcatc aactacagtg ccgctggagt ctgacgcagt gatcacacga
      841 gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga cgttgccgga gccagaaacg
      901 gaaacgcgca tcccttcaaa acccataccg tggcgtttta acattgcttc tgtgaaataa
      961 accagaccgt agccggtagc ttccgggcga ataagactac cgccaaatga aaggccctta
     1021 ccggtgaaga cgcaggcggt attgttggag agctttttca tcatccccgc cataaagccg
     1081 acttcacgac caccaacgcc gatatcacct gccggaacgt cagtatccgc gcccagatga
     1141 cgataaagct cggtcatcag cgcctggcag aaacgcatca cttcgctttc gcttttacct
     1201 ttcgggtcaa aatcgctacc gcctttacca ccgcccatcg gcagggtagt cagggcattt
     1261 ttgaaggttt gttcgaagcc gaggaatttg agaatggaga ggttaactga cggatggaag
     1321 cgcataccgc ctttgtacgg gccgatggct gagctgaact gcacacgcca tgcacggttg
     1381 acctgtacct ggttgcgatc atcaacccat accacgcgaa actggatcac gcgctccggt
     1441 tcaaccagac gctccagtaa tgacatctgg cgatattttg gattttgttc aagaaaaggc
     1501 cagagtgtgg tcattacttc acgaacggct tgcgcgaact cggtttgatt cgggtcgcgc
     1561 ttttggacat ggttgaggaa tgactccaga gaatatgtct gatccataga tataaaaccc
     1621 ttatatatta atacgattgt gcttatgttg tttatgtcat gtgcttttgc agttttcgac
     1681 tataccatca agaatgtgac ggtaagcaag aaaaaaatga cccaggaaag caaattaata
     1741 acgagagtaa tctcataacc aaatgcgata actcagacgc taaagttttg ccacccggct
     1801 accgttatca gttgcagaga caataacgtt aaggagttcc ctgtgagtcg agcattgttc
     1861 gccgtagtat tagcatttcc attaattgca ctggctaacc cgcattatcg tccggacgtg
     1921 gaggtaaacg taccgccaga agtgttcagt tcaggcgggc agtcagcgca gccctgtact
     1981 cagtgctgcg tttatcagga tcaaaattat tctgaagggg cggtaattaa agccgaagga
     2041 atactgctgc aatgccagcg ggatgacaaa acccttagca ctaatccgct gctctggcgg
     2101 cgcgtaaagc cataaacgcc tctaatagtg gaatgtcagt aggggccagc ggatattgca
     2161 gcgcctcttc aggtgagcac cagaccagcg cctgatgttc atgtgcctgt aacgtcccgt
     2221 ggaagtcggg tacgtgccag gcatgaagat ggataatccg gcccgaaact tctcgctgat
     2281 ggctggcaac atactcaccc acagttgctt cgatgcccaa ttcttcgttt aactcacgca
     2341 ccagtgcctg ctgctggctt tcatccagct cgactttacc accggcaaac tcccataatc
     2401 ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat tttgccatca cgttcaatga
     2461 tggcggcaac aacttcaatc attttcataa attacacacg ggcagataag cgaaaaatgc
     2521 cataatatcc gcactgttgc tcaatcagaa aggctttata tttaaggagt gcctgtgcta
     2581 gaccgccatc ttcatccccg gattaaaccg ttgttgcatc agtgcgtgcg ggttcttgat
     2641 aaaccgggca ttacgccaga tggtttaaca ttagtgggat ttgccatcgg cgtgctggct
     2701 ctgccgtttc tggcgctggg ctggtatctg gcggcgctag tcgtcatttt gttgaacagg
     2761 ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag agcttaccga cgcgggcggc
     2821 tttcttgata tttctctcga ttttctcttt tacgcgctgg tgccgtttgg ctttattctt
     2881 gctgcaccgg agcaaaatgc gctggcaggc ggctggttgt tgtttgcgtt tatcagcacg
     2941 ggcagcagtt ttctcgcctt tgctgcactg gcagcaaaac atcagattga taaccccggt
     3001 tatgcacata agtcgtttta ctatctgggg ggattaaccg aaggcacaga aacgatctta
     3061 ctgtttgtgc tgggatgttt attccctgca tggtttgcgt ggatcttcgg tgcgttgtgc
     3121 tggatgacga cctttacgcg ggtgtggagt gggtatctga cgctgaagtc gctccagcgt
     3181 cagtaattgc aatgttattt gctactgtcc gggccgcgtt caccggttgc taccggattt
     3241 tttggatcgc tgctccattc gtaccagccg ccgtcataca cggaaacatt cttccaaccc
     3301 atggcgcgtg cgtacataaa ggtttcggac gcgcgccagc cggtgccgca gtagaatgaa
     3361 acttgctgct ctggtttgat attccacgct ttccacatag cggtaatatc atcggcgctg
     3421 cgcatggtgc catccgggtt atgaaaatct tccatatgcg tcgagtcgct accagcgtgt
     3481 ccccaacgtg ctccggctat ttcacctttt ggtttaatat agctgtaacc gctggtcgta
     3541 ccgataaatt ctggccacga acgaatgctc accagcgatg catcctggcg atgcagcagt
     3601 ccacgggcct gttccatatc gagcatcaac tgcggttgtg ccgggatctt cacgccgaaa
     3661 tccggttccg ctttcacttt cggtggcgtt cctcgctcaa caggcagtcc cgcgtcggac
     3721 caggtttgcc agccgccatc cagcagacgc acatctttca cgcctgcgta gagcataatt
     3781 tgcgccacac gcgctccagc gtatacgtca cgcccgtaca gaatgaccgt ggtgtcatgg
     3841 cgaatgccgt gttttgccag catcgctttt agttgttcat cagaaacttt gttccacagc
     3901 ggttcgcttt ccacttcgtt ggtatcgatg tagtcagcac cgggaatatg gctgataagg
     3961 taaagcttag gagcgcccca ggccgcttca atgactttcc agtcaccggc aggtttcgcc
     4021 gtaacctctt taccttgttg caggtcgtgc agccattgcg gataaaccag ctgctcaaag
     4081 tgcggcagtt tttgtagacg ggaaggttcg cttagcgcgt cactcagaat ggagatatgc
     4141 gtaaaacctg ctttttgcag tcgcgttttg acggcgtcga catctttgtc attaccgtac
     4201 agcgccaccg gggcatcggc tttcaggtta tgttgcttga tccacgcgtt gagctgttcg
     4261 gtgctcattt tgtcgagcca gctggcagag aggtttaagg cggcaggttc atgaccagaa
     4321 gggccattta aggtttgtgg ccagccgtta taaaacgcgc tggggcgagt atctatcgct
     4381 ttgccatttt gttgttgaag ctggtcaagt gtaagaggct tcgccagttc agcggcccac
     4441 gaagaagcgc aagccagccc taaagccatt gccagcgcgg tcatttgaga aacacgtttc
     4501 atcgaaaatc ccgacattgt agttttgagt gcgccattct gaactgacgc attgagaaaa
     4561 actttgcgtt atcgcagttt gttgtaattt tctgaccact gcgccatatc cagaacagaa
     4621 ctatcagcag gaacatcctg gagatcgtgc gttacctgaa cgacggggat cgccagttcg
     4681 cgaacttcgc tgaacaccca ctggcgaaaa ttatcgcgca gagccacatc aagacggctg
     4741 aatggctcat ccaggagtaa cgcttttggc tgagcgagaa gggcgcgcag cagggccacc
     4801 cgcgctcgct gaccgcctga taatgtcgca gggtcctggt gataagtttc agccagtccc
     4861 gctcgatcaa gcgcgtcttt cacggcattg cgcctggcag tccccttgag cgtcgacggt
     4921 agcgccagca gtaaattttg cccgacgctg aactggtcaa ataacagtgc atcctggaaa
     4981 agaatgccaa tctgacgctg tgcggtgggt agcatgtcaa tccgttgctc attgagccat
     5041 agctcacctg tacaagaaaa ctgtccagcc agtgcaccaa tcatccatga aaacagagtg
     5101 gattttccac agccagacgg ccccattaac gtgacgatgt cacctttatc aaccgtaaag
     5161 ttaacgtttg tcagcaagcg gctttctggt aaacgtagcg aaacattttt cacgcagagc
     5221 attagcggag tccttgtctg acataaccta cccattttgc gactaacgcg gtcagggcaa
     5281 aaataataag cggtaacaac agttgccata aagcctgggc ggcgagaata ccgttgctgc
     5341 cgccgctgct taatgccacc gcctcagtgg tgagcgtcgg aaaacgcccc gcgcccagcc
     5401 acagcgtcgg catatactgc gcaatactga ctgaaaatcc caccgcgaag gcaatcagca
     5461 cagggcgcaa catgagtgtg catttcacgt aaaagaagat tttggcccgc gaccagccca
     5521 gtgtttgcgc aatcaaaatt aaccgtgaat caatgcgctg ccaggcgggt tgcaggataa
     5581 acagcatcca cggcattacc cacagcagat gcccccagac caccgccgtc cagcttccat
     5641 ccaggttcag ccatagcgcc agcgtgtact ggcccgccac cagtggcaga gcgggcaata
     5701 gaatgggcaa ccatagccac aactggcgac gctgtggccc ccattccagc cacagcagta
     5761 acaggagcaa agcgatgaat gttgctacca gccccattgt cagactgttg atgagtgctt
     5821 cactattgat cgtcgactga tccgcgagga tcgccagcag aaccacacac agcacaccgg
     5881 ttaagggtaa aaaactcgcc agcgtagtgc ctggtaataa aggtgtggca ggcttgcgaa
     5941 tgccatctac gcggggaata gtgcgccgcc agctgcgcca tagcagatag ctcagcaaaa
     6001 cgtaggcggc gagtaacagc atcagcagca gactggcgag tgcgcctttc gtttgttgat
     6061 ccgcgtcgcc ctgagttaac cactgccagc taattactgc cagcgtcggt ggattacctg
     6121 gcccaagaat aatcgccaca tccacgaccg aaagcgacca ggcaacaatc gccagcatcg
     6181 ccattgccag cgaaggcgcg acggagggca acagcagcca gttcaggcat tgccagcggc
     6241 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttca ctcaatactg
     6301 ccgctaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     6361 tgccaaatcg gtccattggc ggagtgaaat acgggaaata gtcatatagc agccctccgt
     6421 cagcaaagag cagcagggcg ctggtggcaa aagccacatg gggaatggcg agcagccacg
     6481 gcagacgggc gcacatacgc tgccatttcg gcccaggcca tagcgccaca atcaccagca
     6541 gggcaatcaa caatgccccg accgccgcga tggttgtcga caccagcgtt gccagtaatg
     6601 catgcggtaa ctgtggatcg gcaaataacg cctgccagtg tgtcaacgac aacgcaggcg
     6661 atatcagcgt gagagctgcc gggatcagcg gtgcataaat caccgccacc atcgcccaca
     6721 gcaaaaagat taatgcgtac cgtaacggcg taaccattct tgttccagtg cattcaccca
     6781 tcctgcgtgc ggttcagcca gtaccggcgg cagatcctgc ggcattcttg attgcaatat
     6841 ttcgcgctgc ccgtcaggca gtttttgcgg atcgagaaca gaaggatcgt cccagacaac
     6901 gggatctgct ttacgcagtt gcgcatcggg tgagagcaga aaattggcaa ctaccttcgc
     6961 cgcagcactg gcattcgcgt tggctggaat ggtgacgaaa tgcacgttgc caatcatccc
     7021 ctcgcgaaag ccaaaactgt aactgcttgc cggcaaatcg ccgctggcga ttttttgctg
     7081 cgcatgcgcg gggttaaagg tcagcgacag gcgcaatgtg ccggctttca gcagagcatc
     7141 catccgcgcg ggcgaaggcg ggaaatcctt tccttcgcgc cacaaatacg gatgcagtgc
     7201 atcaagatat tgccacaagg gcgcagtgac acgggcgaaa gtcgcatcgt ccggcgcttc
     7261 ttttaatgcg gcgggatctg gcgtcagcat aatcagcaac tgttcaagaa acgccgtgcc
     7321 ggtaaagtcc ggtgggcgcg gataagtaac cgtgccggga ttggctttag caaactccag
     7381 taaggcttgc ggcgtttgtg gtggctgtgg cgtaacatcg cggcgggcga taaacgtcag
     7441 ttgtgcgccg ccccagggcg attccgcacc ttgtgtcggc actgaaaaat cttcccgcac
     7501 cggcagctgt gtgtcgacat agcgccagtt gggcagagtc tccgcccagc ccgtttgtaa
     7561 taaattggcc tcttttaagg tgcggaagtt ttcgccgttc acccagagca gatccaccga
     7621 gccgcccgtt ttacgaccgg cagcagcttc ggtctgaatg cgcttcaccg cgtctgcggc
     7681 atccgcgaga cgaacaatct tcaggtttat agcgtaatgg gttttcatct cgccgctaac
     7741 ccagtcgaga tagcggttaa ttgcggtatc gccgccccag gcgttaaacc agacggtttg
     7801 ccctttggcc tcatttttaa tttcctgcca gtcactggca tgtgttgcca aagaaaacag
     7861 cgataacaat cccagcaacc acccacaatg gcgcatcttc tctccttaag aacgtttttc
     7921 cggaatacgg cacagcccct gagcgggaat gtccatggca gaattaaaac gagccgacag
     7981 gttttgcagg ccgatcaatg ccgtcagttc ggtgagcgcc tgagcgtcaa aatgcgcagc
     8041 cagtcgggta cgcagggcat catcgactgt tggcggtgtg acgcttgcgg cttcggcgta
     8101 ctccagcgcc agccgttctt catcgctaaa gagcgggctt tgccgccagt cagccactgc
     8161 cagcagttta tcggtgctgc cggtacgctc ggcgactttc atactggtga tatccacaca
     8221 aaactcacac aggcacattt gcgcaatgcg cgcgctgaca agcgatcgca ctaccggatc
     8281 gagcggtgag cgtttgcgct ccagccaccc aacaaacatt gacaccaaat aaaagataaa
     8341 cgggatccgc ccccaccagc gaatcgggct tagcacctcg ccgtaatggc gacgatgcag
     8401 ccacgcctgc ggacgtaaaa tgaaaggaat tttgctaagc ggcggtaaac ccatcagtca
     8461 ttcctcttct ggcgctcaag ccatattttc ttcgccataa aaatcaccac ggtcagggca
     8521 aacagaataa acagtccggt gacaaaccag aaagtaccgc ctgttaacat gctgcccgcc
     8581 caggaataaa caatagtcgc cggtaattga ccaagcccgg tggcgataaa aaacgagcga
     8641 aaacgtattg aagtcagacc ggcagcatag ctgattggat cgaaagggac aaaaggcaat
     8701 aaccgacaga ccaggatggt gtgtttgccg tagcgagtga aaaagccgtc catactgtca
     8761 agcacggttt tgccggttaa tttttccacc acttcgcggc ccatcactct ggcgataaaa
     8821 aagcacagcg ccgcgccggc catcgaactg gtccacgaca gcaagccgcc ccagaacgcg
     8881 ccaaacagcg acgcattggc aaaggtgatc aaaaacgcag gtagcggtgc agcaatggcc
     8941 tgtaaaatca tcaacaagaa cgagacaact gccgccagtg cgccgtaaga ctgaataaag
     9001 cgttctatac cctgttggtc cacggcggca aacgccgcaa cgctgcgatt gataaactca
     9061 tggacgccgg gaagcagcgc ccatgcgagc agcatagcga acaacaggat gatgagggtt
     9121 atacagtagt accagatttt acgcgattgc atcttcatca tcctttatcc tggctttatt
     9181 cggtgatttt aaaggtaacc gttgccagcg tgttatccgc cgggagaact gaggcattgc
     9241 ctttgaattt cacttcgccg cgtttttcca ccgcaccata agtgtatgtt gcattgctga
     9301 cgatgccgac cgggcagcta tccagacaca ccagtcaacc cgttttcttc tcttctgctg
     9361 ccgttaaatt accgccaaag cgcatgtcca gtttcttgcc attactgtca acaatcactt
     9421 catcgaagga atacgctttt gccgcccctt gccagttgac cgaaatatcc agtttgctgc
     9481 ctgtgacatg agtcgtttct ttattatcca tcgtcatgtt ttcgcccggc gtaccacctg
     9541 cctctttcag ggcttcataa aatgctttcg gggtcgcata agccataaac agcgatttat
     9601 gtccgttgga gccatcttta aacacaatac cgtgacgagt gtcgtcggtg aggaaacgac
     9661 cattaatttg caccagcatg gttaccgtat ggttattggc atcgacgcgc agcggttggc
     9721 tgttcgaaag tccgtcgtat tccacttttg ccgccgaatt ctctttttga tcgcagccgc
     9781 taagcccggc aacagccagt aagcagagtg cagccagtcc ttttttccat gacactgaat
     9841 aatgttgcga catcgttcta tccttcattt tttgggtgag taagcgggct gcggcgcgaa
     9901 gcagacagat ccacatgttt gtgtcgggcg tagagttttg cgagctggac gagaataaac
     9961 agcgccaggc ccgccagaca gagttgtaaa ataaagcgc
//
LOCUS       DADLTD010000161         9986 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLTD010000161 DADLTD010000000
VERSION     DADLTD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.161"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            289..486
                     /locus_tag="JN701_003141"
     CDS             289..486
                     /locus_tag="JN701_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365497.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(436..1629)
                     /locus_tag="JN701_003142"
     CDS             complement(436..1629)
                     /locus_tag="JN701_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365498.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1765..3489
                     /gene="iucA"
                     /locus_tag="JN701_003143"
     CDS             1765..3489
                     /gene="iucA"
                     /locus_tag="JN701_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY7365499.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3490..4437
                     /gene="iucB"
                     /locus_tag="JN701_003144"
     CDS             3490..4437
                     /gene="iucB"
                     /locus_tag="JN701_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY7365500.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4437..6179
                     /gene="iucC"
                     /locus_tag="JN701_003145"
     CDS             4437..6179
                     /gene="iucC"
                     /locus_tag="JN701_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY7365501.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6176..7513
                     /gene="iucD"
                     /locus_tag="JN701_003146"
     CDS             6176..7513
                     /gene="iucD"
                     /locus_tag="JN701_003146"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY7365502.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7519..9714
                     /gene="iutA"
                     /locus_tag="JN701_003147"
     CDS             7519..9714
                     /gene="iutA"
                     /locus_tag="JN701_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY7365503.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2400 a         2571 c         2656 g         2359 t
ORIGIN      
        1 tgtctggaga ttcagggggc cagtctactt cataaaaaac atgtcagtga aaaagaaacc
       61 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
      121 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
      181 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
      241 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
      301 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
      361 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
      421 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
      481 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
      541 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
      601 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
      661 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
      721 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
      781 agtcaggcgc tgcggacgga ctaccccgaa ctgcgcgata agcgcagcta ctgcggacag
      841 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
      901 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
      961 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1021 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1081 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     1141 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     1201 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     1261 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     1321 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     1381 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     1441 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     1501 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     1561 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     1621 tggtgacatt ataatgcttc aactccatgt attaattgtg tttatttgta aaattaattt
     1681 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     1741 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     1801 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     1861 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     1921 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     1981 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2041 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     2101 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     2161 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     2221 cctaagtctc atgaaccgtt taaccggcgg gaggctgaac gctacctgcc tgatatggca
     2281 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     2341 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     2401 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     2461 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     2521 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     2581 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     2641 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     2701 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     2761 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     2821 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     2881 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     2941 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3001 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3061 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     3121 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     3181 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     3241 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     3301 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     3361 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     3421 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     3481 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     3541 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     3601 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     3661 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     3721 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     3781 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     3841 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     3901 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     3961 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4021 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4081 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     4141 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     4201 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     4261 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     4321 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     4381 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     4441 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     4501 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     4561 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     4621 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     4681 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     4741 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     4801 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     4861 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     4921 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     4981 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5041 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     5101 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     5161 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     5221 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     5281 catgctaccg ggggatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     5341 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     5401 aaccggcggc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     5461 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     5521 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     5581 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     5641 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     5701 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     5761 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     5821 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     5881 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     5941 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6001 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6061 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     6121 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     6181 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     6241 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     6301 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     6361 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     6421 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     6481 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     6541 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     6601 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     6661 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     6721 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     6781 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     6841 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     6901 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     6961 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7021 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaataactc tggaaagtag
     7081 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     7141 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     7201 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     7261 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     7321 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     7381 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     7441 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     7501 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     7561 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     7621 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacttgggt tatcgaaaat
     7681 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     7741 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     7801 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     7861 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     7921 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     7981 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8041 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     8101 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     8161 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     8221 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     8281 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     8341 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     8401 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     8461 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgacg
     8521 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     8581 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     8641 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     8701 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     8761 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     8821 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     8881 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     8941 aatgctggtc tgctgatgca tatcaccgaa cgtcagcagg catggttcaa tttttcccag
     9001 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9061 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
     9121 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
     9181 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
     9241 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
     9301 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
     9361 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
     9421 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
     9481 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
     9541 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
     9601 gtctggggac agcgtgcacc tctgtactac agcccgggtt acggccctgc ttcactgtac
     9661 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
     9721 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
     9781 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
     9841 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
     9901 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
     9961 ctgaaattcc tgagaggatg agctgc
//
LOCUS       DADLTD010000162         9963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.162, whole genome shotgun sequence.
ACCESSION   DADLTD010000162 DADLTD010000000
VERSION     DADLTD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.162"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..236
                     /locus_tag="JN701_003148"
     CDS             <1..236
                     /locus_tag="JN701_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7365504.1"
                     /translation="TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLV
                     YEKTYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            343..1434
                     /locus_tag="JN701_003149"
     CDS             343..1434
                     /locus_tag="JN701_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY7365505.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTCMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAISWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLARFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(1431..2222)
                     /gene="nei"
                     /locus_tag="JN701_003150"
     CDS             complement(1431..2222)
                     /gene="nei"
                     /locus_tag="JN701_003150"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY7365506.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="JN701_003151"
     CDS             complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="JN701_003151"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY7365507.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="JN701_003152"
     CDS             complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="JN701_003152"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY7365508.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="JN701_003153"
     CDS             complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="JN701_003153"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY7365509.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="JN701_003154"
     CDS             complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="JN701_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY7365510.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5601..7082
                     /gene="dtpD"
                     /locus_tag="JN701_003155"
     CDS             5601..7082
                     /gene="dtpD"
                     /locus_tag="JN701_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY7365511.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="JN701_003156"
     CDS             complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="JN701_003156"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY7365512.1"
                     /translation="MTTHLVWFRQDLRLHDNLALADACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8539..9048)
                     /locus_tag="JN701_003157"
     CDS             complement(8539..9048)
                     /locus_tag="JN701_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY7365513.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9333..9719)
                     /locus_tag="JN701_003158"
     CDS             complement(9333..9719)
                     /locus_tag="JN701_003158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365514.1"
                     /translation="MWNDIFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIK
                     YQKYENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFK
                     PAKIAMNKNNLIIRTSDVEIAFKITK"
     gene            complement(9692..>9963)
                     /locus_tag="JN701_003159"
     CDS             complement(9692..>9963)
                     /locus_tag="JN701_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY7365515.1"
                     /translation="TCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHNFIGMGGD
                     YHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT         2309 a         2713 c         2406 g         2535 t
ORIGIN      
        1 aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc
       61 tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca
      121 gcgacatgtt ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc
      181 ccatcgaggc tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg
      241 ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcctct
      301 tttccttgat tactgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc
      361 aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg
      421 ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt
      481 gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc
      541 tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt
      601 tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg
      661 ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat
      721 accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat
      781 tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg
      841 ggggccgctg ttctggtcac ctgcatgatg ctgggtgata acgctgaagc ggttaaccag
      901 caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc
      961 gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg
     1021 ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg
     1081 ctggcgatgg cgtatatggc aattagctgg cggattggtc ttggtttcga taaacaaatc
     1141 ttactgcggg cactacgccc actaccgcaa atcctgctgt cgatttttgc tctgctggct
     1201 atttgtgcag gtatggcgtg gggactggcc cggtttatgc atattgattt tatgactgcc
     1261 tacctcgcca ccagtcccga cgggcttgat accgtggcgg tcatcgccgc agggagcaat
     1321 gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg
     1381 gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg
     1441 cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca
     1501 cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga
     1561 tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac
     1621 gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc
     1681 aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc
     1741 tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc
     1801 tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac
     1861 ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga
     1921 atggttttgt cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg
     1981 ccggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg
     2041 ttggaaaaat gagttaacaa cgccttacca cgcgttccca catgggtaac gtgttgaccg
     2101 ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt
     2161 ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc
     2221 atggtgatat ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct
     2281 tttcggcaaa tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat
     2341 gcaggcagac tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc
     2401 cgtgttgcac catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc
     2461 ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt
     2521 cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc
     2581 caacgagaac caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt
     2641 gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc
     2701 cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa
     2761 cggtttctgg cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg
     2821 ggtgagcgcc aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt
     2881 gggcatcgcc tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc gttaatagct
     2941 ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgtagc
     3001 cgccacgcta attgttcgaa ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc
     3061 agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata
     3121 tcagcctcaa tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc
     3181 aacacaatcg gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa accgtgagat
     3241 aacagttcgc gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg
     3301 ttactttgcg ggctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg
     3361 aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta
     3421 acgccctgcg cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc
     3481 ttcagtaaac ggccttccag cccgccaatc cccacgttga gatcggtgct gcatgacccc
     3541 attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat gccgtgctgc
     3601 gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca agcgccagcc ggtccagacg
     3661 gcgttatcat ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt
     3721 tcgaactcaa ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta
     3781 cccaccagca ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgata
     3841 cccgactggc gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga
     3901 ataatcttca gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta
     3961 ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact
     4021 gccagccacc cggtgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag
     4081 aacctgccgg aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca
     4141 aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg
     4201 aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca
     4261 aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt
     4321 ccagggcctc gctctcctcc caccagcgtt gcaaaagctc tatggcatcc agcgccagcg
     4381 actcaggatt acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat
     4441 tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca
     4501 ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt
     4561 gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca
     4621 tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg
     4681 gcatggtgac ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt
     4741 tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa
     4801 ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca
     4861 caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc
     4921 acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc
     4981 cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa
     5041 tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt
     5101 ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc
     5161 gcgcccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt
     5221 ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc
     5281 gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct
     5341 tccgtattct gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca
     5401 atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc
     5461 acgcttcagc taatgcatat ttatgcaatc agacagcata ttgcataaat aatcagatct
     5521 gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg
     5581 ccgtcaattg tggaattatt atgaataaac acgcatcaca gccgcgcgct atttactatg
     5641 tcgttgcgct gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc
     5701 tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct
     5761 actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg
     5821 gcaatcgcat ggcggtgatg ctgggggcgt tattgatggc gatcggtcat gtggtgttgg
     5881 gtgccagtga gatccatccg tcattcctct atctgtccct ggcgataatt gtttgtggct
     5941 atggtctgtt taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc
     6001 cgcgtcgtga tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg
     6061 cacctattgc ctgtggctac gcccaggaag agtacagctg ggcgatgggc tttggcctgg
     6121 cggcggtggg tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac
     6181 atacccgcgg cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg
     6241 gatggctgct ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag
     6301 agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa
     6361 tttatcgcaa agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca
     6421 ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt
     6481 atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc
     6541 agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag
     6601 aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc
     6661 tgatgagcgc cgggttctgc attctgacct taagcgcccg ctggtccgca atgtatggtc
     6721 actcttctct gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta
     6781 tcgacccggt tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat
     6841 taaccggcat ttatatgctg ctttccggcg cgattgcgaa ctatctcgcg ggcgtgattg
     6901 ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg
     6961 catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac
     7021 tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat
     7081 aataggccag caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg
     7141 cttcttcgta cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg
     7201 gataatccag catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc
     7261 ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt
     7321 tctcgccctg ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatcg gttccggttg
     7381 aagcggccca ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca
     7441 tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg
     7501 taatcatccg tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcagcat
     7561 cgacaatcgg gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct
     7621 gccactgcac acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat
     7681 acgtcatcag atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg
     7741 ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg
     7801 ataacccgcc cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat
     7861 ctcgttgttg ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg
     7921 ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg
     7981 taatggatgg cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact
     8041 ccggcatccc ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt
     8101 acatctcgtg attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc
     8161 cttcacacac cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca
     8221 cttcatactg atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa
     8281 ctatttcgac actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt
     8341 ccgcaagcgc tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg
     8401 acatgttatg cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag
     8461 acgaattgct gcaggcgtca gccagtgcga gattatcgtg caaacgtaaa tcctggcgaa
     8521 accagaccag atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca
     8581 aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt
     8641 gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc
     8701 agccagctgc tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat
     8761 caaaacattg gtgtgatcgc gcgggttggc ggggtgagaa agcaaaacaa ctacgcgttg
     8821 gcggtactgg ttgtaatagt catccagatt ttgccactgg tgaatatcag ccacgaacgg
     8881 gccaagtttg cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg
     8941 ataatcgaga agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag
     9001 ggcaaagata cggattaggg tgctgtcatc aaatcgttgt agattcatta ctgctcctga
     9061 acggtataaa ggagtaagtg tagacgatgg taaatgaatt ttatggggca gagtttttgc
     9121 ggggaagtat tttgttggta aaaatattat caacttcatc tgagcgagaa ttattctact
     9181 atttcccgag ctgcattcat tttttgtatt ttttattttt agaaaataac agttctcaaa
     9241 gcttaattac ctcactatta attgtatttc aaactaacaa tcgcactcca ttttctctct
     9301 ttaaaaaaca taaacactgt taaaataaat atttatttcg ttatcttaaa tgctatttct
     9361 acatcactcg tccttataat caaattattc ttattcatag ctatttttgc aggtttaaaa
     9421 tagcggcttt caccaaagtc agccatgaaa attagactac aagttctccc agcctttgca
     9481 catgagaaaa accacccatc aactctattt attcctgaac ctttaacacc ataataaatc
     9541 agaatttctg ggtattgttc atccttcacg ttttcatatt tttggtattt aattgaaaac
     9601 caagagtcta tactgccaac atcttcaaaa aatattggag ctgatgtttc cgcatgaaca
     9661 tatgcagaaa atgctaacaa aatggcaata cttagtttgg taaaaatatc gttccacact
     9721 tttcaggata atctattggg gggaagggat cttttcctct aaatctaatt gctcccactc
     9781 cttgtttgtc agaccattcg ccatttgcat cctgacgata ccagtgataa tcccctccca
     9841 tgcctataaa attgtgagga cgaataaaca attgagcttt atggtaacca gatggacaac
     9901 tcccattatc taaatccttg gcaccatcat tttttgacgc ttcaaaaata ctaccgcaag
     9961 tga
//
LOCUS       DADLTD010000163         9897 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLTD010000163 DADLTD010000000
VERSION     DADLTD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.163"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN701_003160"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN701_003160"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY7365516.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN701_003161"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN701_003161"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY7365517.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="JN701_003162"
     CDS             complement(2698..3390)
                     /locus_tag="JN701_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAY7365518.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /locus_tag="JN701_003163"
     CDS             3520..5280
                     /locus_tag="JN701_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY7365519.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5686..6543
                     /gene="focA"
                     /locus_tag="JN701_003164"
     CDS             5686..6543
                     /gene="focA"
                     /locus_tag="JN701_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY7365520.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6598..8880
                     /gene="pflB"
                     /locus_tag="JN701_003165"
     CDS             6598..8880
                     /gene="pflB"
                     /locus_tag="JN701_003165"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7365521.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEAR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9072..9812
                     /gene="pflA"
                     /locus_tag="JN701_003166"
     CDS             9072..9812
                     /gene="pflA"
                     /locus_tag="JN701_003166"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY7365522.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2536 a         2489 c         2355 g         2517 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtttc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgtccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgaag atggaataat ccagtatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcaatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgagg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttattcgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcggtgg ttgaagcgat cgaaacactg gaagcagaag gtttcccgat cttcgcatat
     4261 gatggttccc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgtg gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctaatgc
     5461 tcccttttaa ttaactattt tagcggagga tgcggaaaaa attcaactca tttgttaatt
     5521 tttaaaattt atttttattt ggataatcaa atatttactc cgtatttgca taaaaaccat
     5581 gcgagttacg gacctataag ccaggcgaga tatgatctat atcaatttct catctataat
     5641 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
     5701 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
     5761 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
     5821 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
     5881 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
     5941 ggagctgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag tgggcgcatc
     6001 acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
     6061 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
     6121 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
     6181 tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
     6241 cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc
     6301 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
     6361 ttcgcatctc cggaattctg gaccgctgtc ggttctgcac cggaaaattt ttctcacctg
     6421 accgtgatga acttcatcac tgataacctg atcccggtta cgatcggtaa tattatcggc
     6481 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgatcaccat
     6541 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     6601 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     6661 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     6721 tccttcctag ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     6781 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     6841 atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag
     6901 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     6961 tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac
     7021 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     7081 aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac
     7141 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc
     7201 acttctctgc aggctgatct ggaaaacggc gtaaacctgg aacagactat ccgtctgcgc
     7261 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     7321 ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc
     7381 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     7441 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     7501 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     7561 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     7621 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     7681 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     7741 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag
     7801 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact atgctatcgc ttgctgcgta
     7861 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     7921 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     7981 aagtctgaac cgatcaaagg cgatctcctg aactacgatg aagtgatgga gcggatggat
     8041 cacttcatgg actggctggc taaacagtac atcactgcac tgaacatcat ccactacatg
     8101 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     8161 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     8221 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     8281 ggcgaatacc cgcagtttgg taacaatgat ccgcgtgtag atgacctggc tgttgacctg
     8341 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     8401 cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaactgg taacacccca
     8461 gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac
     8521 cagaaaggtg ctgtagcgtc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     8581 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagct
     8641 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     8701 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     8761 aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     8821 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     8881 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     8941 ccttttttag cacgagagcc ttttttgtca gctatctata ctttaaggtg actgccaaaa
     9001 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     9061 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     9121 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     9181 gtcataaccg cgacacctgg gacacgcatg gcggtaaaga agttaccgtt gaagatttga
     9241 tgaaggaagt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     9301 ccggcggtga agcaatcctg caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     9361 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     9421 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg
     9481 agatccacca aaatctggtt ggagtttcca accaccgcac gctggagttc gccaaatatc
     9541 tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     9601 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa
     9661 tcgaactcct cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt
     9721 acaaactcga cggtgttaaa ccacctaaga aagagaccat ggaacgcgtg aaaggcattc
     9781 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     9841 gttgccgctt ttttattccg cgcttcattc tgctacagtg tttttaacac attttat
//
LOCUS       DADLTD010000164         9509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLTD010000164 DADLTD010000000
VERSION     DADLTD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.164"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..191
                     /locus_tag="JN701_003167"
     CDS             <1..191
                     /locus_tag="JN701_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365523.1"
                     /translation="AHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNP
                     LATLPSGDKVAAAVGITP"
     gene            complement(296..832)
                     /locus_tag="JN701_003168"
     CDS             complement(296..832)
                     /locus_tag="JN701_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7365524.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            916..2124
                     /locus_tag="JN701_003169"
     CDS             916..2124
                     /locus_tag="JN701_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365525.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2124..2939
                     /gene="ybjI"
                     /locus_tag="JN701_003170"
     CDS             2124..2939
                     /gene="ybjI"
                     /locus_tag="JN701_003170"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY7365526.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3025..3309
                     /locus_tag="JN701_003171"
     CDS             3025..3309
                     /locus_tag="JN701_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365527.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="JN701_003172"
                     /pseudo
     CDS             complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="JN701_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4865..5461
                     /gene="ybjG"
                     /locus_tag="JN701_003173"
     CDS             4865..5461
                     /gene="ybjG"
                     /locus_tag="JN701_003173"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7365528.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5519..6277
                     /gene="deoR"
                     /locus_tag="JN701_003174"
     CDS             5519..6277
                     /gene="deoR"
                     /locus_tag="JN701_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY7365529.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6324..7526)
                     /gene="dacC"
                     /locus_tag="JN701_003175"
     CDS             complement(6324..7526)
                     /gene="dacC"
                     /locus_tag="JN701_003175"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7365530.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKDVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7820..8398
                     /locus_tag="JN701_003176"
     CDS             7820..8398
                     /locus_tag="JN701_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7365531.1"
                     /translation="MLTLEELELPYEQILAGREFGINHDADFLAMNPNGLVPLLRDDE
                     SDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWANQTLSNAHRGILMGLV
                     RTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFGVGDIAIAPFIYNLFNV
                     GLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(8395..9486)
                     /locus_tag="JN701_003177"
     CDS             complement(8395..9486)
                     /locus_tag="JN701_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7365532.1"
                     /translation="MPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLPDNHGMLIT
                     LRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWLSYSEVGDD
                     GKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLFIALGENN
                     QRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGMAMNPWSN
                     ALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGTEQPVFYW
                     KDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRILTDRGQRI
                     RDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2303 a         2457 c         2345 g         2404 t
ORIGIN      
        1 cggcgcactt catgatgtcg ggtggaaaca ttctggcctt acaaaaaatt ctcggacacc
       61 acgatataaa aatgactatg cgttacgcac atctggcacc ggatcatctg gaaacggcgc
      121 tccgtttcaa tcctctggca acgctgccaa gtggcgacaa agtggcggca gcggttggca
      181 ttaccccgta ataaccacca ctgaccacca acctaactta ttgtttttaa tctaacttat
      241 tgttttcatt aacccgttta cataaatggg ttttttgtta tctaaaattt atctactacc
      301 ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga
      361 caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa
      421 accattgttc gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata
      481 gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg
      541 caacctgtga gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa
      601 catcgctaaa aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct
      661 ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg
      721 caagggtagc aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca
      781 gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc
      841 ctctgtctgc aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt
      901 attttgacgt gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg
      961 gcgctgttta tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg
     1021 cctgctatcc gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt
     1081 ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg
     1141 acacgtaatg tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt
     1201 ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca
     1261 agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga gcgagaaatg
     1321 aataaaacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct
     1381 ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg
     1441 gcgctggtag gtatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc
     1501 aaaaatgctg ccgatggcac ccagcatggc gaaaaaggca ttccttttta tcgcgatatc
     1561 cagttgctgt tgattggtgt tgtagtgctg gcgatggcct ttgccgaagg ttctgccaac
     1621 gactggttac ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg
     1681 atttatgccg gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc
     1741 gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttgggtatt
     1801 gggctgatta tttttgtcga tagcgcctgg gtcgctggag tgtctgttgt actctgggga
     1861 ctgggtgcct cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat
     1921 gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg
     1981 ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg
     2041 gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg
     2101 cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta
     2161 ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa
     2221 tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct
     2281 ctttcttccc tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag
     2341 tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg
     2401 tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct
     2461 atacgctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc
     2521 tggaatacgt tgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc
     2581 tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta
     2641 tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag
     2701 ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct
     2761 ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg
     2821 aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg
     2881 aaggcgtact ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa
     2941 aacggcagca cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta
     3001 acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt
     3061 ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga
     3121 tacaaagtgg ttgtagttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact
     3181 gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat
     3241 gctcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat
     3301 caccattaat tcgagaatgc ctgatcgcac aaagcagtca ggcatttttt tacccttcgt
     3361 gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc
     3421 cgttgacaag gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc
     3481 tgatttcaat accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg
     3541 tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac
     3601 caataccgaa agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga
     3661 taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg cccataatta
     3721 tcagcgaacg tacggtgcga cgcgaggtca gacgcgctaa cagcaagtta cccgcaatta
     3781 acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg
     3841 taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca
     3901 gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga
     3961 gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcata gctcgttgca
     4021 gaccgaaaaa ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca
     4081 gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt
     4141 tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaagattcc tgaattgcgg
     4201 cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagagtga
     4261 attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac cacaccaccc
     4321 ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta
     4381 aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct
     4441 gatattgttc caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa
     4501 aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg
     4561 gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa
     4621 aagcgcagct aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac
     4681 attattgcga gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc
     4741 acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc
     4801 tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga
     4861 agttatgctg gaaaatttga atctctctct attctctctt attaacgcga cgccagactc
     4921 ggctccgtgg atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc
     4981 gctgctggcc gcggtgcttt ggttgtgggg gcttacagca caacggcaac tggtgataaa
     5041 aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc
     5101 gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga
     5161 ctcattccca agcgaccacg gtacggtgat tttcaccttt gcactggcat ttttatgctg
     5221 gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg cctggtcgcg
     5281 cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat
     5341 tggctgcctt agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct
     5401 gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg tgcgtgactg
     5461 atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat
     5521 ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa
     5581 gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga
     5641 tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgca
     5701 tagtgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg
     5761 ggcggcaaaa ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg
     5821 caccaccacg ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg
     5881 ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg
     5941 cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc agcaaacgtt
     6001 gaataatttt tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg
     6061 cgctacctgt tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca
     6121 aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtccgtccgg cgcgcatggg
     6181 tgacctgaaa cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa
     6241 gtacgcgcag acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt
     6301 cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc
     6361 atcatcacga aatcccacat ccgaccaaag aatccgccct cttccacatt ttccatcacg
     6421 atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc
     6481 tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg
     6541 tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc gagattcact
     6601 tcgctcttat caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc
     6661 gtcacggttt caaagaagcg gaaaccccag gtcaataatt tctcagactc attaaaacgg
     6721 atacggtcag ttttcgcccc caacactacg gagattaaac gcatatcgcc ctgggtagcc
     6781 gaagcaacca gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc atcaacattc
     6841 agattgctgc tccacagcag acggttacgg ttaggctgac gaattttgtt gaaggtgaac
     6901 tctttctctt tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc tttacccagc
     6961 aatgccatat cgcgcgcggt actgaactgc cccggcgcat ccaggccgtg caccgtctgg
     7021 aaggtagtgt tggtcagacc cagttttttg gcataaccat tcatcagacc aataaatgac
     7081 tcctggctcc cggcaacgta atcggccagc gcaatacagg cgtcattacc ggactggata
     7141 atcacatctt tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag gaacattacc
     7201 gatgaaccac gcagtgccgg atttcccgtc gcccaggcat ctttaccgac cgtcaccata
     7261 tcggtgagtt taatcttatc ggccttaagc gcctgcccaa ccacatagct ggtcatgatt
     7321 ttagtcaggc tcgcgggatc cagtttctca tccgcattgc cttctgccag cactttaccg
     7381 ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc aacggtttgt
     7441 tcggccgcga atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc tgcaagacca
     7501 cgaaggagag aggagtattg cgtcataaat gccacccgag tatccattca aaaaaaatac
     7561 gttatatcgc cgtatgataa gttcggtgat taataacgca cacacaaact taaagaaacc
     7621 gtaagttggt aaagttttta aagtttatgc aatatcctgc cgcttcgctg cttctggtgt
     7681 catttttttg ccagctgttg cttaaaagtt aactttactt caccatcgga gtcctgttca
     7741 gcctgtggct gcctgtcgga aaaggggtga gtatgattac gctgtggggt cggaataatt
     7801 caaccaacgt aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat
     7861 tctcgcgggc cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa
     7921 cgggctggtg ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc
     7981 cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc
     8041 acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac cagacgctca gtaatgccca
     8101 tcgagggatc ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat
     8161 tgatgccagt tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa
     8221 agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat
     8281 ctacaatttg tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta
     8341 tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt
     8401 gcgtgggcta actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc
     8461 gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc
     8521 ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc
     8581 aataaataat ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct
     8641 cacagcgggc gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc
     8701 tttcgcttcc ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc
     8761 gtagtttttg cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt
     8821 cagccacagt gcattactcc acggattcat cgccattcct tgcggattac gaatgccata
     8881 agaccagatc tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc
     8941 gccctgatcg gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg
     9001 gcgctgattg ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg
     9061 cccgccaaaa tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc
     9121 ggtcactttt gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc
     9181 atcatcgcca acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc
     9241 taaaaccacg tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag
     9301 cggcgcagat aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat
     9361 taacataccg tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg
     9421 cagtacttcg acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag
     9481 gggcacaagg aaaaaggatt gtcgatgca
//
LOCUS       DADLTD010000165         9489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLTD010000165 DADLTD010000000
VERSION     DADLTD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.165"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1168)
                     /gene="emrB"
                     /locus_tag="JN701_003178"
     CDS             complement(<1..1168)
                     /gene="emrB"
                     /locus_tag="JN701_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY7365533.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSG"
     gene            complement(1185..2357)
                     /gene="emrA"
                     /locus_tag="JN701_003179"
     CDS             complement(1185..2357)
                     /gene="emrA"
                     /locus_tag="JN701_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HAY7365534.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(2484..3014)
                     /gene="emrR"
                     /locus_tag="JN701_003180"
     CDS             complement(2484..3014)
                     /gene="emrR"
                     /locus_tag="JN701_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY7365535.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            2985..3086
                     /locus_tag="JN701_003181"
     CDS             2985..3086
                     /locus_tag="JN701_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365536.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(3105..3440)
                     /gene="ygaH"
                     /locus_tag="JN701_003182"
     CDS             complement(3105..3440)
                     /gene="ygaH"
                     /locus_tag="JN701_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY7365537.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(3430..4167)
                     /locus_tag="JN701_003183"
     CDS             complement(3430..4167)
                     /locus_tag="JN701_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY7365538.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(4291..5475)
                     /locus_tag="JN701_003184"
     CDS             complement(4291..5475)
                     /locus_tag="JN701_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365539.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(5767..6759)
                     /gene="proX"
                     /locus_tag="JN701_003185"
     CDS             complement(5767..6759)
                     /gene="proX"
                     /locus_tag="JN701_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY7365540.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(6817..7881)
                     /gene="proW"
                     /locus_tag="JN701_003186"
     CDS             complement(6817..7881)
                     /gene="proW"
                     /locus_tag="JN701_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY7365541.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(7874..9076)
                     /locus_tag="JN701_003187"
     CDS             complement(7874..9076)
                     /locus_tag="JN701_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY7365542.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
BASE COUNT         2397 a         2490 c         2454 g         2148 t
ORIGIN      
        1 aaccagacag cgtaatggtg gtcagcggca taaagaagca ggccaccgca aacccctgga
       61 taaactgcgg ccaggccgac gcgccaaaat ccatacctgg ttcaaaggta taggcacgcc
      121 agtagaagca gacggcatac ataataaagc tgaaggttac cagccgccgc atatccagtt
      181 tatgcgcgaa gcggccgata atcggcgaca ggatcaccgg aataatccct accggcgcag
      241 aggccaaacc tgcccaggtc gccgtgtaac cgtagacctc ctgcaacaac tgcggcagca
      301 gaacaatcgc gccgaagtag agcatatacg cgaggctgat acacaagcag ccgatggtga
      361 agttgcgcga cttaaacaac gacagatcga ctatcgggtt atcgtcggtc agctcccaga
      421 caatcaggaa gcagatagcc accaccgcca ccacggtaag aatgataatt tcctgtgatg
      481 aaaaccagtc cagctcttta ccgcggtcga gcataatctg caggctaccg ataccaataa
      541 ccagcagtgc cagccccacg gcatcaatcc gccgccgttc ggtgcgggtt tcgcgtccgc
      601 gcagggtttg cagtgtcatc aacaccaccg ccacgccaat tggcacgttg atgaagaata
      661 tccagcccca gtggtaatta tcgctgatat aaccgccgag gattgggccg caaattggcg
      721 cgacaatcac cgtcatcgac cacaacgcca gcgcgattga gcgtttggct ggcgggtaat
      781 tattcagcaa aagactttgc gaaagtggga tcaacggccc agcgacaatc ccctgaatca
      841 cacggaagaa gatcagcata ttcaggctgc tggagacacc acactcccac gacgcaatag
      901 caaaggcgat ggtggaccaa aggaacagtt tcacttcccc gacgcgcttt gccagccagc
      961 cggtaagcgg gatcgagatg gcattcgcca ccccgaaaga agtgattacc cacgttccct
     1021 ggctgagcga tgagcccaga ttcccggcga tagtggggat cgccacgtta gcaatggtgg
     1081 agtccagcac ctgcatgaat gtcgccagtg acagcgcaat cgtcataatg accagttgcg
     1141 cgccttccag cggtttttgc tgttgcatca cacgcacctc tggattagcc agcgttagct
     1201 tttacgatat cgtcgatcag tttattgaca ggtgccaggc tgatttcacg cgcggtactt
     1261 accgcgaccg gtgttgaacg tactttattt gccagtacct gaccgtcacg gttagtggta
     1321 ttgacgctca ccagcgtgga caaaccaatg cgcagcgggt attgctccag ctgtttctgg
     1381 tccagttcga tacgcacagg tagacgctga acgactttga tccagttacc ggtcgcattt
     1441 tgcgccggaa gcagtgagaa tgcgctacct gtgcccatat ccagaccaac cactttaccg
     1501 gtgtatttca catcatcgcc gtaaatatcc gtggtgatag tgaccggctg accgatacgc
     1561 atattggcaa tctgcgtctc tttaaagttg gcatccaccc acatattggt ggctggaacg
     1621 accgccatca gcggcgtcgt tgggctaatt tgcgccccag gctgtaccgc gcggcgggag
     1681 acataaccgg tcatcggact gacaatacgg gtacgctcca gcgccagcca ggcgttacgt
     1741 acttcggtgg cagcctgttg cacggcaggc tgatcttcca gtttagtccc cagaatcatc
     1801 gcctgattgg cattgtattg ttgaatagcg acgtccagtt gcgcctgggc actggtgacg
     1861 gcgtcgcggg cgtgttgcag ctcttcgcga ccaatcaggt tggcattgcc cagcggcaca
     1921 cggcggttgt agtcgctttg tgctttcgcg agggcgattt tttgcacctc aatattcgcc
     1981 tgtaactgtt tgctgttaat catcagctgg tgggtttggc gaacgctgga agccagtgca
     2041 gttttggctt tttcaaacgc ctggcgagca tctgtcgggt cgagagtgac cagaacatcg
     2101 ccttctttta caaaatcggt gttatcggcc cagactttcg tcacgctgcc agacacctga
     2161 gacataattt gaatctgatt ccctgccacg tatgcgtcat cggtttcttc gaaatgacgc
     2221 agtaccaaaa accaataaat ccctatcgct acggcaataa ttataaagag caaggtgaga
     2281 aggaggagca gacgcttacg tttgccgctc ttctttaccg gttgctgcgg ggtttgagtc
     2341 tccgcatttg cgctcatatt gttctccacg atcttcttaa ctcatcggct gagccgacct
     2401 gcttgccaaa aaggccagca tgttgcgatg ctggccggtc attttttctt ttataaatct
     2461 ggatttttga gcgagatgac gcgttagctc atcgcttcga gaaccacacc gtcttgttcc
     2521 atctggtcga gacgggagag caatttgcgg gtgatttgct cgagctgatc tttttctgtt
     2581 gtgctgagcg cggaccagag ttgatgcagg cagttatgct gcggtggtaa aacctcgcgc
     2641 aaaaactcgt gacctttttc cgttaattgc agatgcaggc agcggcgatc gttatcgctt
     2701 tcacgacgtt cgatccaacc gcgtttttcc agttcatcgg caatacgcgt cgcgttggta
     2761 cgggatgatc caagagcaca acttaattca gaaggctgaa tactgtggtt ttcctgagac
     2821 tccagcgtaa tcaacgccat aaacaacgtc tcgttaatcc cctgagcctt cagcatttta
     2881 ttgcggttct ccagcagctt gctttgcatg tgcatgcaaa gacgagtcag aaggatctcc
     2941 tgataaggaa aatcttcgtg gcggctggcg cgaaatttta gcatttgttc aatgggcgta
     3001 aacgaactat ccatttgggt atgacctcat taatttcagc cgatatagta acgacagtaa
     3061 caaataaagt aaatgtattg ttttagaaaa atgattcttg tgggttatat aatcgccatc
     3121 actttccagg cgagcccata ggccagcgca ctaagcagtg ttgggatgat aatgctgcgt
     3181 gttttataga aactggcacc cagtaccgcg aagccgacca gcgtgggcac gaaacggcgt
     3241 gtatcgtgca tcacttctgg tgcggtagag acaaccagca gagcgcatat cgaggcgatg
     3301 ccaatggtgt cgagcaaaat acctaccgcg ccacgtttgg ttgggcgggc attacccaca
     3361 cgcaggcgca gcggcaaata gcggaagcaa taattcgcca cgccaaccag taacccaagc
     3421 agcagaacct catagctcat cgggcgctcc ttgccagaat gcctggatta acgcagtgag
     3481 gcagccacag acaatgcctg ccagaatggc gacgggaata gaaaatagcg ttacgcctgc
     3541 aagggcacca actaacgctg cggtaacgca aagagattgt ttgcgctgga aagaggcgag
     3601 caggaaactc ataaagagtg ccggaagcat aaaacctaat gcagcttcaa cggcgggata
     3661 accttgcagc aagccgctgc cggagaatgc ccctattacc gtaccaaata cccacgatga
     3721 ccatgaactg aaggcaatgc cgatcatcca gttctcgctc cagcggcgat tattgcgtac
     3781 cagttttgcg gttgcggcgg caaaaacctc atccgtcagg ccaaatgccc acagggcggt
     3841 tttcgatttt tgcagacgct gaataatacg gctacgcagt gacgggccat acaacacatg
     3901 gcgaacatcc attgccatga cggtcagtgc agcaacccac aaactactcc cggctgccag
     3961 catcgcggta atgacgaact ggctcgcgcc tgcataaatg atgcaggaga aaaaaacgct
     4021 ttcgagagga gagaatccca gacgggtcgc attcagaccg aacgcaaagg ccaccggaat
     4081 ataactaata acaatcggta aactgtcttt gcatccttcc atgaaggtcg ccgaaccaag
     4141 agcaggctgt ggagtagggc tttccataga gtgtacgctt aacgattgtt aatattataa
     4201 atagactgaa tgaatatctt aaccttatca gactgatggg cttcttaaca cccttataag
     4261 tgtaaagcca cgaaaacggt tgctgattgc ttaatttgcg gcttcctgac gatgaaaacc
     4321 tcgccaccag accagtaagt taaccagggc aatcgtcgcg ccagccagac aaacgccagc
     4381 ccaaccgcca tgttgccagg ctgaggctga aattagcgaa ccggcggcac cgccaataaa
     4441 gtagctggtc atgtaacctg cggtcaggcg attacgcgca tcagggtgta ttcgataaat
     4501 taccgtctgg ttagtgatat gcacgccctg tacggtgaga tccagcacca ggatgccgat
     4561 aatcagcgcc agtacggaag tgtgaccaaa ccagatcgcc agccatgaaa gtaatagcag
     4621 cagcagaccg aaagttgtgg tgtggtgcga tttaccctta tcggcaaaac cgcccgccgg
     4681 acgagcgccc aacgctccgg cagctcccgc aagtccaaac agaccaatga caccatcgct
     4741 gtagttaaaa ggtggagcgg caagcaaaaa ggccattgag gtccagagaa tgctgaagtt
     4801 ggcaaaggtc aggcagccca gcaatgcgcg ggtgcgcagg atcttatcgc tgataaacat
     4861 gctgaaaacg gaacctaaca actgtgggta gttgaggtgg gtttctgatt tcatttgtgg
     4921 cagaccacgc cataatgcca gcgccatcag tgccattaac accgaggcaa cccaaaagac
     4981 ggtgcgccag ccgccgagat tcgccagcaa tccggcaacg gtccgtgcga gtaagatccc
     5041 caacagcaga ccgctcataa tagtgccaac cactttgccg cgtttgtccg gtgaagccag
     5101 cgtcgctgcc agcggaacca gaatttgtgc cacgacagag aataaaccgg ttaatgccgt
     5161 accgaggatc atcatcgcca gcgactgact gctggcagtg attaacatgc cgcctgccgc
     5221 cagtaaggtc atcgagacaa tcaggcggcg gcgttcaaac atatcaccga ggggaacaag
     5281 aaacagcaga cctgctgcat agcccaattg cgcggcggta acaataaagc ctgccgaact
     5341 ggcggaaagg gaaaagttac gcgcgatggt gtcgagcaat ggctgggcgt aatagttact
     5401 ggctaccgcc agaccggtgg cgatagacat cagcacgatc agcgccgggc taagctcatg
     5461 attaggttta gtcattattt ttgttcatca ggtgatgatt aagctggaaa tcaataatag
     5521 cggatgttga tgaatatggg atgaaagata gcttgttgaa atgtgcgatg tggtttgtag
     5581 gcatgataag acgcgccagc gtcgcatcag gcattgtgca cagaacggcg gatgcggcga
     5641 aaacgcctta tccgccatac ggaatgagtg tattttgtag gcatgataag acgcgtcagc
     5701 gtcgcatcag gcattgtgta cagagcggcg gataaggcaa aaacgcctta tccgcctgaa
     5761 taaaaattac ttctgcgctg ccagcgcctc attcacccag ccatcgaact gctgctggtg
     5821 ggctttgatc cagccatcaa cgtggccctg aatatcgcct tctgaggctt tgccgtcatg
     5881 cataatggcg ttctgggcgt taatatcggc cactggcaac tgcataatgg caaacagttt
     5941 ggctgccgcc ggatttttct cggtccaggc tttgttggca acgatatgca tggtgctgac
     6001 cgggaagccg taattcgcgc cattcggcag tttggtatcg gcatttttat cgcccggcag
     6061 tgcggagaac ggcacctgca accagaccac atctttccct ggcttcagct cattactcac
     6121 ccagtacggc gtccaggtgt aataaaacac cggtttgccc tctttgtagc gactgatggt
     6181 gtcggccatc atcgctgcgt agttcccctg attatgcgtc acggtgttgg tcagttcata
     6241 cgcggcaagc tggtggttga tcgcaccttc gcagccccag ccaggattac aaccggttaa
     6301 atccgcttta ccgtcgccgt tggtatcgaa cagtttggcg atcttcggct ctttcagttg
     6361 tgcgatgttg gtgattttgt actggtcggc ggttttctta tcgatcaggt aaccctgtgc
     6421 cgcgccgtta acaaataccc cttcacgata aaatttctta tcgccaccgg cagcttcgta
     6481 catgttgtca tgcagtggcg tccagttcac ggcggtgaag gttgcatcgc cggaagcaag
     6541 caaggtgtag ccaacgttgt aatctacttc gctgggtttg ttgacggtat agcctaattt
     6601 ctccagcgca cggctgacca gcagcgtctg gaaggtttct tcggtgatgg tgctttgaac
     6661 tggattaaca gtaatacctt tgcccggcag atcggcagca aaagtttgtg tagagataag
     6721 cgtggcaaac gctgtcgcaa aaagtacgct atgtcgcatt gttattcctt tttttggcag
     6781 ggtgataatg cccggcgtca ccgggcaagt gcagagttac ttaatgaatg ggcgggtcag
     6841 cagaccaaca gggccagtgg tgtaccagcg acggttgccg cgactgcgtg agtcgcgccc
     6901 aacggcctgc gtcaggcgat cgaggataat ggcgaggatc acaatcccga cgccgccaac
     6961 ggtggcaagc cccatatcca gacgaccgat accgcgaagt accatctgac ccagcccgcc
     7021 gacggcaatc atcgaggcaa tgaccaccat agaaagggcc agcatcagcg tctggttaac
     7081 gcccgccata atgctcggca tcgccagcgg taactgaact ttgaacagca tctggcgcgg
     7141 gctggcaccg aatgagcgcg aggcttcaat cagatccgcc ggaacctggt taatccccag
     7201 aatggtcaga cggataatcg gcggcagagc aaagatgatc gtcaccacca cgcccggcac
     7261 gttaccgata ccaaatagca tgacgattgg caccagataa acaaacgctg gcgtggtctg
     7321 catggcatca agcagtggac gaataatttt cgccgctcgc ggacttctcg ccagccagat
     7381 ccccagcggc aaaccaatga cgatacagaa cagcagggcg gttaacacca gcgccagggt
     7441 aaccatggcc tgcgaccagg caccgattgc gccgatggca atcagcgaaa ccagcgtcgc
     7501 cacgcccatt ccgaccccgg aaatctgcca ggcgatgaga gcgaaaacga taatcgccac
     7561 cggtgcgggc atacccagca gcaattgctg gaaaccgttg aggatataat caaccggaac
     7621 gcgcacgccc tggaagacgg gacggaaatg ggtaacaacc cagtcgatcc cttcagtgac
     7681 ccaactgtcg agcgggatca gcgttttatg gaacggatcg agaatattaa aatgctcgac
     7741 gtttggcgca ggcgtactgg tcagccagtc agcaccaccg ccgtcagtcg gtgcagtcgc
     7801 cggtgtaccc caggcgtctg cggattgcac ggcactgtcc gccgctggcg tggtatccca
     7861 cggattattt tgatcagcca ttatttaccc cctcacgatc taaagcgcgc agcagcattc
     7921 ctttcgaaat gatgccgaca tactgttggt cctcgtcgac cacgggtacc gcacagggtg
     7981 cctgtccgac atgagagagc aactcgctaa gaggcgtttg tgcatcgact gctaacggcg
     8041 catcaatcag cgccgcatca agaccttgct gctgcgttaa cgcggtttta agcgaatcga
     8101 tggaaactgc gccgacaaac ttattaccgc gttcgataac gtagccatat tcgcgatctt
     8161 catcctgcaa taatttcagt gccgaacgtg ggccgaagcc aggggtttta cgaattaagc
     8221 cattcggtgt ccggcgggca atatctttcg cactgaatac ctgactaata tcaacgccac
     8281 ggaagaaggt acggacataa tcattcgccg gattattgag aatttcatcc ggtgtgccga
     8341 cctgtaccac ttcaccattt tgcataatgg caattcggtc gccaatacgc atagcttcat
     8401 cgagatcgtg ggaaataaag acaatggtgc gctgatgttt cgcctgtaat tttaccagct
     8461 catcctgcat ctcggtgcga attaatggat cgagcgccga gaaggcttcg tccattaata
     8521 atatatccgg attaatcgct aacgcgcggg ctaatcccac acgttgacgc atcccgccag
     8581 agagttcatc cgggtagctg tgggcataat tttccagccc gacctgacgc agtgcatcaa
     8641 gggctttttc ccggcgttct tcggcattaa ttccggccaa ttccataccg aacgcagtat
     8701 tgtccagcac ggtcatatgc ggcattaagg caaaggactg gaagaccatc gcaatctttt
     8761 ttctgcgcac ctcacggagt tcggcatcgg atattttggc aatatccaca ccatcaatca
     8821 gcacttgccc gcgggtgggt tcaatcaggc gattgagaag gcgtaccatt gtggatttac
     8881 ccgagccgga taatcccatg atgacaaata tctcgccttc ttcaatggcc agactggcgt
     8941 cttttacgcc aagcgatagc ccagtttttt ccagaatttg ttctttcgaa agtccttgtt
     9001 cgatatattt gaacgctcgc tgtggatgct cgccaaatat tttataaaga tttttaattt
     9061 ctaatttaat tgccatgcaa tagaaagatt cctttatttg tttatgtcga tatattaccc
     9121 aatggaaata gtcacttttt tctaccctaa catactgaga atctgaggca acccctgagg
     9181 gcaaatgtgg catgaatatt gatgcgagca aatcgccgga atttcccgtg atataagggc
     9241 tgagagcaaa tcgagaaaag ttgatatttt ttgtcataga tagcgaaaaa acgcctgaat
     9301 attgtagttt caggcgaata atgcaacaga gtctcagtgt agatcactac agaatatttg
     9361 cgtgataaaa agctatttgg cgggaattat ttccctgctg cgggtagtga tatttttgaa
     9421 aataacaccc tcagaaattc cagtcttcat cttctgtttc aaccgctttc cccatcacat
     9481 aagaggagc
//
LOCUS       DADLTD010000166         9377 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLTD010000166 DADLTD010000000
VERSION     DADLTD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.166"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(311..889)
                     /gene="mocA"
                     /locus_tag="JN701_003188"
     CDS             complement(311..889)
                     /gene="mocA"
                     /locus_tag="JN701_003188"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAY7365543.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            992..1762
                     /gene="yqeC"
                     /locus_tag="JN701_003189"
     CDS             992..1762
                     /gene="yqeC"
                     /locus_tag="JN701_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAY7365544.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1810..3435
                     /locus_tag="JN701_003190"
     CDS             1810..3435
                     /locus_tag="JN701_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HAY7365545.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3476..4408)
                     /gene="arcC"
                     /locus_tag="JN701_003191"
     CDS             complement(3476..4408)
                     /gene="arcC"
                     /locus_tag="JN701_003191"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY7365546.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNVLREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4456..5824)
                     /gene="hydA"
                     /locus_tag="JN701_003192"
                     /pseudo
     CDS             complement(4456..5824)
                     /gene="hydA"
                     /locus_tag="JN701_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
     gene            complement(5877..7088)
                     /locus_tag="JN701_003193"
     CDS             complement(5877..7088)
                     /locus_tag="JN701_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAY7365547.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7146..8342)
                     /gene="dpaL"
                     /locus_tag="JN701_003194"
     CDS             complement(7146..8342)
                     /gene="dpaL"
                     /locus_tag="JN701_003194"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAY7365548.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8400..>9377)
                     /gene="ygeW"
                     /locus_tag="JN701_003195"
     CDS             complement(8400..>9377)
                     /gene="ygeW"
                     /locus_tag="JN701_003195"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HAY7365549.1"
                     /translation="TRTRFSYASALNLLGLAQQDLDEGKSQIAHGETVRETANMISFC
                     ADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQCDIDHPTQSMADL
                     AWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFGMDVTLAHPEGYD
                     LIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKVMEERTELLRAND
                     HEGLKALEKQCLAQNAQYKDWHCTEEMMELTRDGEALYMHCLPADISGVSCKEGEVTE
                     GVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQERVK"
BASE COUNT         2195 a         2286 c         2456 g         2440 t
ORIGIN      
        1 tagcttatac agatatcatt gagtgaccgg tgttgagaaa tccggtggtt tagggggata
       61 gagcaacaat cgcaccactc ttgccaggtt tggcgggaaa tataaaaaaa tgtgaggcgg
      121 ctcgaaactt aagggctttt tgtgaatgaa ctcacaggtg ctaaatgaga attaaaatca
      181 ctcctgaaaa ttattattgc cagcccatat atcacttttt ctcttttctc aaaatcagtg
      241 attccttatc attatgataa agagtgctgg agggattaaa tatttgaacg caatcgttta
      301 actacgtttt ttaaatttta gtataccttt tttgcactgt aataaagtcg tccggagtat
      361 caatatctaa aattatttct gcattttcta cttcgacgga ataatgagct cccataagga
      421 gtgcctgacg catattggtg atattggggc gcttgattgc ctgcatcagg catggttttg
      481 acactaaaat cggatggccg gggatgccat tatggagtgg cagtattgcg ccatcatttc
      541 gtaacgacca gatttttctg aaaatatcga tggtgagggt tggcatgtca ccgtgggtga
      601 gaaaacaatg ttctgtttgc actgcgggta ctgcggcctt tactgatgtt agtaaaccct
      661 gcgcataatc tgggttgtgg ataatagtga tattgctctg gttcacataa cgttcgtgca
      721 gttcattacc acgatagccg gtgactaaaa taattcggct acaaaactgc aacgcatttt
      781 tgatacttgt atcaagaatt gtcccctgtt gccagggtaa catcattttc cattgcccca
      841 ttcttgatga taatccggca gcggtaatta tacagtcgat ggctgacata gtggttccat
      901 tatttatagg ggctaataac gcctgataat tgcgttactt cctgatggat taagttgggg
      961 ataatttatc ccagagaggt cataaagact cgtgaaaagt atagttgacc catcggcatt
     1021 attcattgat ttaggtgcgc agaaacgtcc cactgtgatt tctgttgttg gtgctggggg
     1081 gaaaaccagc ttgctttttt ggctggcaga actgctccag gctagcggca ggcgtgtgtt
     1141 aatcacgacg acaacacata tgtttatgcc aacgtctcac tggcccgtgg ttttctgtcg
     1201 tgatcccgcc atgcttcctc atgcgtctct tacatccccc atttcatttt gttttcacag
     1261 ctggaaagcg aaccagggaa aagtgcaggg atttacgcca gaagcgattg atgcactggt
     1321 gcaacgacca gagtgtgacg taattctcat tgaagcagat ggctcgcgtg caatgccgtt
     1381 aaaagcgcct gatgagcacg aaccttgcat acccaaaagc agttgctgcg tgattgctgt
     1441 gatgggagga catattttgg gcgcgaaagt gagcacagaa aatgtccatc gctggtcgca
     1501 gtttgctgat attactgggt taacacctga tgcaaccttg caactgagcg atctcgttgc
     1561 gctggttcgc caccctcagg gggcgtttaa aaacgtaccg caaggttgtc ggcgagtctg
     1621 gttcattaac cgtttttctc aatgtgagaa tgcgattgca caaagcgagc tccttcaacc
     1681 gctgcaacaa cacgacgtag aggcaatctg gctaggcgat atacaagaac atcctgcaat
     1741 cgcgcgcaga tttgtgaatt agcggcccgg gtattccggg ttactgaaag gatatccatt
     1801 gaggttggta tgaatatttt cacagaggct gcaaaactcg aagagcaaaa ttgtccgttt
     1861 gcgatggcgc aaattgttga tagccgaggc tcgactcccc gccattctgc acaaatgtta
     1921 gtgcgcgccg atggttctat cgtcggtaca attggtggcg gaatggttga gcggaaggtg
     1981 attgaagagt cacttcaggc attgcaggaa cgtaagccgc gattattcca tggacgtatg
     2041 gctcgtaacg gtgcggatgc tgtcgggtca gattgtggag gtgcaatgtc agtatttatc
     2101 agcgtccatg gtatgcgtcc acgtctggtg ttgattggcg cggggcatgt caaccgggcg
     2161 atagcccaga gtgccgcgct attaggattt gatattgccg ttgccgatat ttatcgcgaa
     2221 agcctcaatc ctgaactatt cccaccatca accacgcttc tccatgctga gtcgtttggt
     2281 gcggcagtgg aagcactgga tattcgccct gataattttg tcctgattgc cacgaataat
     2341 caggatcgtg aagccctcga caaactcatt gaacagccca ttgcatggtt ggggttgctg
     2401 gcaagtcgcc gcaaggttca gcttttcctg cgtcaattgc gtgagaaagg cgtggctgaa
     2461 gaacatattg cccgtttaca tgcgcccgtt ggttacaaca taggtgcgga aacgccgcag
     2521 gagatcgcca tcagcgtgct ggcagaaata ttacaggtga aaaataacgc gccgggtggg
     2581 ctgatgatga aaccttctca tccttccgga caccagctgg tggtgattcg cggtgcggga
     2641 gatatcgcca gtggtgtggc gctacgtctg tatcatgcgg gttttaaagt gatcatgctg
     2701 gaagtggaaa aaccgacagt gattcgttgt accgtggcgt ttgcccaggc cgtgttcgat
     2761 ggcgaaatga cggtcgaagg cgtcactgct cgcctggcaa ccagctctgc ggaagcgatg
     2821 aaacttaccg aacgcggatt catccctgtg atggtagatc ccgcctgttc attgcttgat
     2881 gaactgaaac cgctttgcgt ggtggacgct attctggcga aacagaattt gggaacgcgg
     2941 gcagatatgg caccagtaac aatcgcgctt gggccgggct ttactgcagg gaaggattgt
     3001 catgcggtaa ttgaaacaaa tcgcgggcac tggctcggtc aggtgattta ctctggttgt
     3061 gcgcaggaga ataccggtgt tcctggcaat attatggggc ataccacccg acgggtgatc
     3121 cgtgctcctg ctgcaggcat tatgcgatcc aacgtgaaat taggcgatct ggtgaaagag
     3181 ggcgatgtga ttgcctggat tggtgagcat gaaattaaag caccgttgac ggggatggtg
     3241 cgtggcttgt tgaacgacgg cctggcagtg gttggtggtt ttaaaattgg tgatatcgat
     3301 cctcgtggtg aaaccgctga tttcaccagc gtttctgata aagcccgggc gattggcggc
     3361 ggcgtacttg aggcgttaat gatgttgatg catcagggcg tgaaagcgac aaaagaagtg
     3421 ctggaagtgg cttaaatgaa aatggccgga tggtattacc cacccggcct taaaattact
     3481 gacaaatcac cgttccggtt ttgccttcaa tcccttcttt cgctttgctc aatacggtaa
     3541 tcagtgcttc acgacctgcg cgagagcgag caaacgaggc ggcagcttcc actttaggca
     3601 gcatagaacc tttcgcaaaa tgaccttctt cgataaagcg ttcagcatca cttaatgaca
     3661 ggcgatcgag ccactgttca ttctctttac cgaagttaat ggccactttt tctaccgcag
     3721 tcagaatgat cagcatatcg gcatcaatca tttctgctaa acgggcgctg gcccagtctt
     3781 tatcgataac cgcgctggca ccacgcagat ggttaccttc acgaataaca gggataccac
     3841 caccgccaac ggtgatcacc acctggcctg catctaccag agctttaaca gtttcttttt
     3901 caatgatatc aaccggtttt ggcgaggcaa caacacggcg atagccgcga ccagcatctt
     3961 ctttcagagt gtaaccctgt tttgtcagtt gctctgcttc ttgctcggta aagaacgaac
     4021 cgatcggctt ggtcgggttg aggaatgccg gatcgttagc gtcgacttcg acctgagtaa
     4081 ccagcgttgc tacaggttta ttaatgcccc gagaaagcag ttcttctctt aacacgtttt
     4141 gtagatcgta accaatataa ccctggctta aggcaacaca gacagacatc ggcagcattg
     4201 gcgagtgtgc ttcagttttt gcggcagctt caaaggcctg gttaatcatg cccacctgcg
     4261 gcccgttacc atgagtgacg ataacttcat gtccttgggc aattaaatca acaattgcct
     4321 gagaagtaat ttttaccgct ttcatttgtc cggccaggtc atcgcccagc gcattcccgc
     4381 ccagggcgag aacaattttc ttactcattt ttcctcactc atcattcttg attgtgtata
     4441 gatccctgac agtggttaga gcacgggagg gacaaacggt ttgcgtcgca ggaaacgacc
     4501 tcgcccggct ttgcctgtaa aggtgccgtc acagaaaatc gtttcaccac gggataaggt
     4561 tctgacaatc gcgccctgac aggtaaaacc ctcccatggc gagtagtcgg cgttgtcgtg
     4621 gagatggcga tgctgaattt gttggctctg acgtgggtcg ataatcacca cgtcgccatc
     4681 ggaaccgggc gctaataatc ctttttgcgg ccacaggcca aacagcctgg cgggcattgc
     4741 gctggttaat tcaacaaagc gttccggtgt tatacgtccc gtcatcacgc cactggaaaa
     4801 cagtaactgc atgcggtttt ccacaccggg taagccattt gggcagcgac tgaaatcgcc
     4861 tttagaaatt tgcaggcgtt gagccatcga gaaggtgcag tgatcggtcg ccaccacgtc
     4921 aatcgcacca tcgctgatgc cacaccacaa cgttacgcag cggtgggcta agaatgaact
     4981 tcatgccatc ttctgtatcg taactgcgtt cgtccaacag gagatattgt gggcaggttt
     5041 caacccagac tggctggtga ttcgcacggg caagacgcag ataatccaga cctaagccgt
     5101 tagacaggtg cacgatatag agcggggcgt taccggcaat ttgtgccagg ttaatcatgc
     5161 gggcgatggc ttccgcttcg cattccagag ggcgactcaa ggcatgatag cgcggcgcgg
     5221 ttaaccctgc ggcgataaat tccgcccgct tgctggcgat agccgcatca ttttccgggt
     5281 gcacggtggt cagcgcgccg gattcatgca agcggcgtaa tgcctgcaaa acctcgtcat
     5341 cgttgagttt gtattgatag gttaaataga gtttaaaact gctcagtcct tcctcgacca
     5401 tcatcgggat ttcgtcgagg attgcgtgat taatgtgctg gatcacaccg tgaaagctgt
     5461 aatcgatgac cgctttatgg gcggcataac cacgataaac ctccagttga tggcgtaacc
     5521 aacagccgtt tgggccaaat cccatatggt caataatggt tgttgtaccg ccacacgcag
     5581 ctgcgcgggt accggtaaaa aaatcatcac aactgcgcgc gatgccgaca tcaatattga
     5641 aatgcgtatg gacatccacg ccgcctggga aaacgtaaca gccagtggca tcaatttctt
     5701 catacgggag ctgcggcgaa atattgttgc ccaactggcg aacaatcccg ctttcaatca
     5761 gcaaatcctg tttggcttgt ccatctgcgt taacgacagt gccgtttttg atcaatacgc
     5821 gcatagcaaa ctccaaaaac cctccggcga accggagggg aataagtgaa gagtaattac
     5881 tctgttgcca gccagcttaa cgggattgca gcgtacatcg cggcacaggt caccaggtga
     5941 gatttccagg ttttttcgtt aggtgcatgc gcttcaggtt ctttacccgg gccaaagccg
     6001 atcaccggaa tgccgtgacg gcccatgata gatacgccgt tagttgagaa ggtccactta
     6061 tcaacaaccg gcgctttgcc aaacagccct tcgtaggcat tcaccagtgc tttaacggtg
     6121 aagtgatctt cttccacttt ccaggtcggg aagtagcatt cggttgggta aaccaggcca
     6181 gtccaggacg gacggtcgta gttgtacata gaaacaaccg cgttagcttt ctgtactgca
     6241 ggcagggcgc ggatttcgtc cagcgcgcct tcccaggttt cgccccaggt cagacggcgg
     6301 tcaatagaga cggcgcagct atctgctaca gcgcaacggc ttggggatgt gaagaagatt
     6361 tcagaaacgg tgagggtgcc tttgccgagg aattcgtcat aacccagacg ttgggagagt
     6421 tcttgtaatt cgccaagaat cggacccatt ttgaaaatgg cgttgtcacc gcgttctggc
     6481 gcagaaccgt ggcagctaac accctgaaca tcaatacgaa tttccatacg accgcgctga
     6541 ccacggtata tctggcagtc ggttggttcg gtactgacca caaattccgg gcgaatgccg
     6601 gattgttcaa taatgtactg ccagcacaga ccgtcgcagt cttcttcctg cacagtaccg
     6661 gtaaccagca gggtatattc atcttccaga ccgaggtctt taatgatttt accggcataa
     6721 accatagatg ccatgccgcc ttcctggtcg gaagtaccgc gaccaccgat tagctcatca
     6781 gtttccatgc cttcgtacgg atcgaagtcc cagtttttga tgttgccaat gccgacggta
     6841 tcgatatgag cgtccattgc caccagacgc gggccgtggc cgatataacc gagaacgttg
     6901 cccatcgggt cgatttcaac tttatcgaag ccgacttttt ccatctcttc tttaatacga
     6961 tgtactacgc gtttttcgtc gcagctttca ctgggaatag caaccatgtc gcgcaggaag
     7021 cgagtcatgt ccgcctggta atcttttgct ttttcaagaa tcagtttgaa tggaatattc
     7081 ttagccatta tttttctcca gattttttac tccccgtttt gcagggagta gttccaatag
     7141 ggtgattaag gtgctacagc gtgtttgcct tcccagacaa cttcgcggta gtgcttcacg
     7201 tcggtgtcgc cttcagtact gataaccagc actacggcat ctttgttcag cgccagtttt
     7261 tccatcaggc tttgacgttg cgggtgataa tgaaccgctg cgagaacgcc caaaccgaca
     7321 gcgccggatt caccggagat gatgcgcggg tcgttgccgt acggattacc cagcacgcgc
     7381 atacctaatg cggcaacgct gtcctggcag gagatgaatt gggtggcgca gttacgtagg
     7441 atttcccagc ccagcgggtt aggttcgcca caggccaggc ccgccatgat ggtggccata
     7501 tcaccgccaa cgttgacgat gtcgcctttg acgccggagc gataaataca gtcagctttg
     7561 tcaggttcaa caataatgct gtgcagattt tgcgggctgt agacgtcgac cagataaccc
     7621 agcacaccac cggccattgc tccgacaccg gcttgcagca gaacgtgcgt cggggttacg
     7681 cccatttcac gcatttgctc gacggcttca tctgccaggg ttgcgtagcc ttgcatgatc
     7741 caggttggga ttttggtgta accttcccat gccgtgtcct gtaccacttc ccagccgtgc
     7801 tgctgcgcgt gttgcatggt caggcgaacg gtatcgtcat agttcatatc cgtgacgatg
     7861 cactcggcac cgaggttcag aatggcgtca acgcgttcct gagcagaacc tttcggcatg
     7921 taaatcaccg cattctgtcc gagttgctgt gctgcccacg ccacaccgcg cccgtggttg
     7981 ccgtcggtgg tggtcgcgaa agtcattttt tcgccgatgg catttttcag gtgctcaaat
     8041 gacagcgttt cgatatcaag atgatatttt tcacacaata attgagcgat ggcgtacgca
     8101 ccgccaagca ttttgaaggc gttcagaccg aatcgttttg attcgtcttt gacgagaatt
     8161 ttcttcacac caaaaaggtt tgcgagatcg tccagcgcac aaagcggtgt tgggcgataa
     8221 ccagctattt tctggtggaa ctggcgcgcc agtttggcct ggctttgcga aaagagcggt
     8281 gatgtttcgc cgttgaaaaa tttgttatcg gcgatatcaa tcttcaatga gaaaacggac
     8341 atatcctatc cttttggcct ctggaaatag agggccgacc agctgatcgg cccgaggggt
     8401 tatttcacgc gttcttgcgc ttctttcagc agttgctcga gcagtgcacc tggtttggcg
     8461 tatttacggg acaggatcat cgcggcgatg atataaggct tccagctggc ttctttgtag
     8521 gtagcgatac ggtatttttc gaatacgcct tcagtcactt caccttcttt gcaggatacg
     8581 ccgctgatat cagctggcag gcagtgcatg tacagggctt cgccatcacg ggtcagttcc
     8641 atcatctctt cagtacaatg ccagtctttg tattgcgcgt tctgtgccag acactgtttt
     8701 tccagtgctt ttaagccttc gtggtcgttc gcacgcagca attcagtacg ctcttccatc
     8761 actttgtaag gtgcccatga cttcggataa acgatgtctg cgtctttgaa ggcttcttcc
     8821 atgctggtga cctgacggaa gctaccaccg gaggctttag cattgttttt cgccacttca
     8881 accacatccg ggatcaggtc gtagccttcc ggatgggcca gggtgacatc cataccgaag
     8941 cgagtcatca gaccgatgat gccttgtggt acagagagcg gtttgccata gcttggagag
     9001 taggcccagg tcatggcgat ttttttacct ttcaggtttt ccagtgaacc aaagtgttca
     9061 cgtaaccacg cgaggtcagc cattgactga gtcgggtggt caatatcgca ttgcaggttc
     9121 actaaagccg gacgctgtgg cagtacaccc tgcttgtaac catcatcaag tgcagcgcca
     9181 acttcacgca tataggcgtt gcctgcgccc agatacatat cgtcgcgaat accaatagcg
     9241 tcggcgcaga aggagatcat attggcggtt tcacgcacgg tttcgccgtg agcgatttgt
     9301 gatttgcctt cgtcgagatc ttgttgtgca aggccgagca ggttaagcgc ggaagcataa
     9361 gagaagcggg tacgggt
//
LOCUS       DADLTD010000167         9065 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLTD010000167 DADLTD010000000
VERSION     DADLTD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.167"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..407
                     /locus_tag="JN701_003196"
     CDS             <1..407
                     /locus_tag="JN701_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7365550.1"
                     /translation="PIFARVNKAGTPVAGLIIVGILMTIFQLSSISPNATKEFGLVSS
                     VSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTTIAFLYCIWAVVGSGAKEVMWS
                     FVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            511..2154
                     /gene="eptA"
                     /locus_tag="JN701_003197"
     CDS             511..2154
                     /gene="eptA"
                     /locus_tag="JN701_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY7365551.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            2151..2819
                     /gene="basR"
                     /locus_tag="JN701_003198"
     CDS             2151..2819
                     /gene="basR"
                     /locus_tag="JN701_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY7365552.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            2820..3920
                     /gene="pmrB"
                     /locus_tag="JN701_003199"
     CDS             2820..3920
                     /gene="pmrB"
                     /locus_tag="JN701_003199"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY7365553.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(3917..4033)
                     /gene="pmrR"
                     /locus_tag="JN701_003200"
     CDS             complement(3917..4033)
                     /gene="pmrR"
                     /locus_tag="JN701_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY7365554.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFL"
     gene            complement(4099..5601)
                     /gene="proP"
                     /locus_tag="JN701_003201"
     CDS             complement(4099..5601)
                     /gene="proP"
                     /locus_tag="JN701_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY7365555.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5865..6748)
                     /locus_tag="JN701_003202"
                     /pseudo
     CDS             complement(5865..6748)
                     /locus_tag="JN701_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6745..8973)
                     /gene="crfC"
                     /locus_tag="JN701_003203"
     CDS             complement(6745..8973)
                     /gene="crfC"
                     /locus_tag="JN701_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY7365556.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2299 a         2411 c         2229 g         2126 t
ORIGIN      
        1 caccgatttt tgcccgtgta aataaagcgg gtacgccagt ggcggggctg attatcgtcg
       61 gtattttgat gaccatcttc cagctcagca gcatttcacc aaacgcgacc aaagagttcg
      121 gtctggtttc ttccgtctcg gtcatcttta cactggtgcc atatctttac acctgtgcgg
      181 cgttactgct gctcggacac ggtcactttg gtaaagcacg cccggcatat ctggcagtta
      241 ctaccattgc cttcctctac tgcatctggg ccgtggtggg gtccggagcg aaagaggtta
      301 tgtggtcatt tgtcaccctg atggtcatca ccgccatgta tgcgctgaat tacaaccggc
      361 tacataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatcc
      421 agcaaacata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
      481 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct
      541 ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt
      601 gcctttttta aacaggtgtt gcaggcgctg ccgctggatt cgctgcataa cgtactggtt
      661 ttcttgtcga tgccggtcgt cgctttcagc gtgattaata ttgtcctgac actaagctct
      721 ttcttatggc ttaatcgacc actggcctgc ctgtttattc tggttggcgc ggctgcacaa
      781 tatttcataa tgacttacgg catcgtcatc gaccgctcga tgattgccaa tattattgat
      841 accactccgg cagaaagtta tgcgctgatg acaccgcaaa tgttattaac gctgggattc
      901 agcggcgtgc ttgctgcgct gattgcctgc tggataaaaa tcaaacctgc cacctcgcgt
      961 ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactact gattttgctg
     1021 gtcgccgcac tgttttataa agactacgcc tcgttgttcc gcaataacaa agagctggtg
     1081 aaatccttaa gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga
     1141 ctggcaaatc taccgctggt gcgaattggt gaagacgccc accgcaaccc gttaatgcag
     1201 aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac
     1261 ttctccctca acggctaccc gcgtgaaact aacccgcggc tggcgaaaga taacgtggtc
     1321 tatttcccta ataccgtatc ttgcggcacg gcaacggcag tttcagtacc gtgcatgttc
     1381 tcggatatgc cgcgtgagca ttacaaagaa gagctggcac agcaccagga aggcgtgctg
     1441 gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa
     1501 ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tggtcagtgc
     1561 atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac
     1621 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac
     1681 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag
     1741 acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     1801 attgttgata aagcgattaa tctgctgaaa gaacatcagg ataaatttac caccagcctg
     1861 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     1921 ccttatgcca tcgccccgga tagccaaaaa caggtgccga tgctgctgtg gctgtcggag
     1981 gattatcaaa aacggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     2041 cactattcac aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag
     2101 tattaccagg ctgcggatga tattctgcaa acttgcagga gagtgagtaa atgaaaattc
     2161 tgattgttga agacgatacg ctgttattgc agggactgat tctggcggcg caaaccgaag
     2221 gctacgcgtg cgatggcgtg acaaccgcgc ggatggcgga acaaagcctt gaagccggtc
     2281 attacagcct ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg
     2341 cccgtatccg gcagaaaaaa tataccctgc cggtactgat cctcaccgct cgcgatacgc
     2401 tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga ctatctggtg aagccttttg
     2461 cgctggaaga gttacacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     2521 aaagtgagct gattgttggc aatctgacgc tgaatatggg tcgccgtcag gtatggatgg
     2581 gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca
     2641 aagcaggcag tccggtacat cgggaaattc tctacaacga catctataac tgggacaatg
     2701 aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaat
     2761 cccgtatccg caccgtgcgc ggctttggct atatgctggt cgcgaatgag gaaaactaat
     2821 tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacta
     2881 tcggggccat tttgttggtg tttgagctga tcagcgtctt ctggctatgg catgaaagta
     2941 ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     3001 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca
     3061 gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgcccg ctggcggagc
     3121 tgcaaaaaga gctggaagcg cgcactgccg acaacttaac gcccattgcc attcacagcg
     3181 ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt cgcctgacca
     3241 gcacgctgga taacgaaagg ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac
     3301 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     3361 cgttagtggc acggcttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc
     3421 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     3481 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat
     3541 tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     3601 tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca aggcagcaac attatgatta
     3661 agctgcaaga agatggcgga gcggtcatgg cagttgaaga tgaaggacca ggtattgatg
     3721 agagtaaatg cggggagttg agtaaagcgt ttgtacgtat ggacagccgt tatggcggga
     3781 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     3841 tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcaga
     3901 acgtggcaaa ccagatctaa agaaagctgc tgaccaccag cacgctgaac acggtagtta
     3961 aactttcata taaacacggt ttttcattgc actctcctcc gttaacctgg aggagagtat
     4021 gcgcgtcaga gattaagtga accttaagag ttcaattggc ctgataagac agcgtcacat
     4081 caggccatcc gtttcagctt attcatcaat tcgcggatgt tgctgcacca ggcgggtacg
     4141 tttcgcctgc aaatcggcaa tctcgtggtc aatattatcg attttctgct cgatattatc
     4201 gtaatgctcg acgagaattt ccttcgcttc ctgtatatct gacgccgccg gtgtcgcacc
     4261 tttcaacgga cgatttgccg tctctttcat ggttacgccg gtgattaaac caatcaccgc
     4321 cactaccatc aggtaatagg caggcatcat cagattctgc gagctttcga ccagccaggc
     4381 cgccagtgtt ggcgtcagac cggcaaccag caccgaaata ttaaatgccg ccgccagcgc
     4441 gctgtaacgg atatgcgtcg ggaacatcgc tggcaaggta gaagccataa cgcccgtaaa
     4501 gcagttaagg atcaccgcca gcatcagtaa cccggcaaaa atcaggccga tgacgttact
     4561 gttaatcaga ataaacgccg ggatcgccaa cacaaacagg gcaacactac caagtagcac
     4621 aaacggacga cggccaaaac ggtcactcag caagcccatc accggctgga caaacagcat
     4681 accgatcata atggcgataa taatcagcac cccgtggtct tcggagtaat gcaggttatg
     4741 cgacaaataa ctcggcatat aggtcagcaa catgtagtaa gtcacgttgg tggcaattac
     4801 cagaccaata catgtcaaca ggctgcgcca gtatttagtg gcaatctctt taaacgagac
     4861 tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc agtttatcaa catgctgctg
     4921 gaacgccgga gtttcttcca gcgcatggcg caggtaaagc ccgataatcc ctaacggcag
     4981 agcaataaag aacggaatac gccagcccca gtcgaggaag ttcgcttcgc cgacaatggt
     5041 cgaaattaac accaccacgc ccgcacccag cacaaacccg gcaatagaac cgaagtccag
     5101 ccagctgccc ataaagccac gtttacggtc aggggagtat tccgcaacaa atatcgacgc
     5161 cccggtatat tcaccgccga ccgagaaacc ttgtgccatc ttacagatca acagcagaat
     5221 cggtgcccaa ataccaatcg tgtcgtagga cggtattaag ccaatacaga acgtactgat
     5281 cgacataatc acaatagtga tagcgaggat cttctggcga ccatatttat cgcccaacat
     5341 accaaagaag agtccgccaa gcggtcgaat cagaaaggga acggagaaag tggcaagtgc
     5401 agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa actttaccta atgcgtaagc
     5461 aacaaaacca taaacaccaa aatcgaacca ttccattgca ttacccagtg atgctgcggt
     5521 aatggcttta cgcagtttac tgtcattaat aatggtgaca tcacgaaggg taatcggttt
     5581 tacttttttc cttttcagca tagctttcct cgcagagttg gcccatttcc gcaaacgcca
     5641 gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt gcaggatgca atctctgtaa
     5701 caagcgtagc gggtttgctt acaccctccg gttaaggaat gtacaatcaa accagttgac
     5761 gcctgaattg ggcagttaat gaccttttta ccctaccagt atttaaattt gtgatcaact
     5821 tcacacatgg atttcgttta ggactcattg atgtaactga ttatttacgc caaatttaca
     5881 gagaagattt cctcgaccat tgcccccacc aaacgctgcg ctgaacagag tcgtggcatc
     5941 cccagcgcga cagtttgcca acgttgggtc actgaccagc aaacgggttg tttatctgcc
     6001 gtggtccact ttcccagcca acttaaaaaa tcagcatgat ccccgcccgt cggaacggtc
     6061 gaagaggcaa accactgatg gtaatagtga cgcgtggtcg gggccgcatt gatactctct
     6121 gccagatgtt gtgtcgcttt cacgcgcaaa tcacccagca gcaacgccac cgcttgcggg
     6181 cttcccagac gacaggcatc gccaaatgcc ccccaacgca actcttcgag aacgataaaa
     6241 cagcgcccag gcaacgacca ggcgttatac gccccagcac gccagcgggt aaaaatttgc
     6301 tcgctctgat cacctgcctg caccagtagg cctggcctcg ttgcgttaac gccttttcct
     6361 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
     6421 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
     6481 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
     6541 gcaggctaag cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg
     6601 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttgt ggaaggcggg
     6661 catcatcgcc ctgcgccaga tccactaaaa agcggggata aaccgactcc agcacgcgac
     6721 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
     6781 atcgcgtaac agctgggcgt cggtgtaaat ccagccgtga gtcatcacag tctgctgcaa
     6841 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca ggctttcgcg
     6901 taaacgcgtc tgtaattccg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
     6961 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaataaag
     7021 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
     7081 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
     7141 aacgcctaaa cgcgtgctct gctcgtccgt tgcctcctgg cgcgaaatga cttcactgaa
     7201 cagctggcga gtgttgacgt ggggaactac tccgtgaaaa acaggaaaat gaaacccagg
     7261 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatgggcc gaataacatc
     7321 ggttaacacc cggcaaaggg tggattcaag ctcggcaaaa cgcagcgtaa agtcgcgact
     7381 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcag gtacgaatct cgcttaacgg
     7441 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc agcgcatctt gctgttgacg
     7501 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
     7561 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
     7621 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
     7681 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
     7741 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
     7801 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
     7861 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
     7921 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
     7981 aaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
     8041 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
     8101 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
     8161 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
     8221 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
     8281 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
     8341 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
     8401 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
     8461 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
     8521 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
     8581 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
     8641 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
     8701 accaacaatg gcattaatgg tggttgattt ccctgctttc atagtaccga caatcgccag
     8761 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt aacgttcggc
     8821 gatattaggc tgactccagg gtaaagccag ttgtgccgca tcgtctccgg gtacagagag
     8881 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
     8941 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
     9001 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
     9061 agaaa
//
LOCUS       DADLTD010000168         9013 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLTD010000168 DADLTD010000000
VERSION     DADLTD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.168"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            195..1028
                     /gene="ppsR"
                     /locus_tag="JN701_003204"
     CDS             195..1028
                     /gene="ppsR"
                     /locus_tag="JN701_003204"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY7365557.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDRIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1184..2230
                     /gene="aroH"
                     /locus_tag="JN701_003205"
     CDS             1184..2230
                     /gene="aroH"
                     /locus_tag="JN701_003205"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY7365558.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2362..2553
                     /gene="hemP"
                     /locus_tag="JN701_003206"
     CDS             2362..2553
                     /gene="hemP"
                     /locus_tag="JN701_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY7365559.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2557..3993)
                     /locus_tag="JN701_003207"
     CDS             complement(2557..3993)
                     /locus_tag="JN701_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY7365560.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="JN701_003208"
     CDS             complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="JN701_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY7365561.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWSAVPINQITTLVQR"
     gene            complement(5015..5479)
                     /locus_tag="JN701_003209"
     CDS             complement(5015..5479)
                     /locus_tag="JN701_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY7365562.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="JN701_003210"
     CDS             complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="JN701_003210"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY7365563.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVGLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="JN701_003211"
     CDS             complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="JN701_003211"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY7365564.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="JN701_003212"
     CDS             complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="JN701_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY7365565.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(8109..8513)
                     /locus_tag="JN701_003213"
     CDS             complement(8109..8513)
                     /locus_tag="JN701_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365566.1"
                     /translation="MAENVAHISRFRKKKENAYNVSNNQVTGSGNDGGDGMNDDLERR
                     VSCLESDVTEIKNNLITLTTRSESFATKSDVLEIREGLRLEMADLRQSLKVEMAEHRT
                     ELQKSFANQTWLLTGIVLSAMAVLVAVVTVIK"
     gene            complement(8513..8875)
                     /locus_tag="JN701_003214"
     CDS             complement(8513..8875)
                     /locus_tag="JN701_003214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365567.1"
                     /translation="MKFHLDELKRMATLDNFARFLNESSTNEKCLSCGDTDMYMYLTN
                     IVEVGPEPKTAEECDLGTFVMLDYIGPFTGYPGYEGHDRENIHNYEFRLTCNRCGFVH
                     RYSARAFMNWVGKQGDEK"
BASE COUNT         2383 a         2386 c         2042 g         2202 t
ORIGIN      
        1 gttgcgcaaa cctgctctat tcgacaattt ccacataaac catcggtata aacgaaacca
       61 taaaaatgaa atgctgtttt cataaaaaat aaaattgaag gttcatttta taaaccagac
      121 attacctcct acgcttctgt gcgtttttaa tttatgcttt catagaatta tgtctgcatc
      181 acgggaagaa caaaatggat aatgctgttg atcgccacgt tttttatatt tctgatggta
      241 cggcaataac tgcggaggta ttaggacacg cagtaatgtc acaatttccc gtcactatca
      301 gcagcattac gctgccgttt gtcgaaaatg agagccgtgc acgggcagtg aaggatcgga
      361 ttgacgcaat ttatcaccag acaggtgtgc gcccgctggt cttctactcc atcgtgttgc
      421 cggagattcg cgccatcatc ttgcaaagtg aaggcttttg ccaggatatc gttcaggcgc
      481 tggttgcccc gctacaacaa gagatgaaac tggatccaac gccgattgct catcgtaccc
      541 atggccttaa ccctaataat ctcaataaat atgatgcgcg cattgcggcg attgattaca
      601 cccttgccca cgatgacggc atttcgttgc gcaatctgga tcaggctcag gtgatcctgc
      661 tcggtgtttc tcgctgtggt aaaaccccca ccagtctgta tctggcaatg caatttggta
      721 tccgcacggc aaactacccc tttattgccg acgatatgga taatctggtg ctacccgcgt
      781 cgctcaaacc gcttcagcat aaattgttcg gcctgactat cgacccggaa cgtctggcgg
      841 cgattcgcga ggaacgtcgg gagaacagtc gctatgcctc gcttcgtcag tgcagaatgg
      901 aagtcgcgga agtggaagcc ttgtaccgta aaaatcagat cccgtggatt aacagtacca
      961 attattcggt agaagagatt gccaccaaga tcctcgatat catgggcctt agtcgccgaa
     1021 tgtactagag aactagtgcg ctggtttgtt ttttgttatc atgctaacca cccggcgagg
     1081 tgtgacacac ctcgcacttg aaatcagctg cgattggttt atcgtgatgc gcatcacttc
     1141 ccggtagtcc tgccgtagaa gcaacaaatt tctgagactt gtaatgaaca gaactgacga
     1201 actccgtact gcgcgtattg agagcctggt aacgcccgcc gaactcgcgc tacggtatcc
     1261 cgtaacgcct ggcgtcgcca cccatgtcac cgactcccgc cgcagaattg aaaaaatact
     1321 gaatggtgaa gataagcgac tgttggtcat tattggcccc tgctcgatcc acgatctcac
     1381 cgctgcaatg gagtacgcca cccgtctgca gtcgctgcgc aaccagtacc agtcacggct
     1441 ggaaatcgta atgcgcacct attttgaaaa accacgaact gttgtcggct ggaaagggct
     1501 aatctccgat ccggatttaa acggcagcta tcgggtaaat cacggtctgg agctggcgcg
     1561 caaattactt ttacaggtaa atgagctggg tgtccctacc gcgactgagt ttctcgatat
     1621 ggtgaccggt cagtttattg ctgatttaat cagttggggc gcgattggcg ctcgtactac
     1681 cgaaagtcag atccaccgcg aaatggcttc agcactctcc tgtccggtag gttttaaaaa
     1741 tggtaccgat ggcaacacgc ggattgccgt ggatgctatc cgcgcagccc gcgccagcca
     1801 tatgttcctc tcgccagata aaaatggtca gatgaccatc tatcagacca gcggcaaccc
     1861 gtatggccac attattatgc gtggcggcaa aaaaccgaat tatcatgccg atgatatcgc
     1921 cgcagcctgc gatacgctgc acgagtttga tttacctgaa catctggtgg tggatttcag
     1981 ccacggtaac tgccagaagc agcaccgtcg ccagttagaa gtttgtgagg atatttgtca
     2041 gcaaatccgc aatggctcta cggcgattgc tggaattatg gcggaaagtt tcctgcgcga
     2101 aggaacgcaa aaaatcgtcg gcggtcagcc gctcacttac ggtcaatcca ttaccgaccc
     2161 gtgtctgggc tgggaagata ccgaacgcct ggtcgaaaaa ctcgcctctg cggtagatac
     2221 ccgcttctga atgcgtgccc attcctgatg ggatgggcat ttctgcgcaa cttgttgtct
     2281 tctcaacaca ttactgcttg ctctggcctg ccataatatt gataataaga atcattgtta
     2341 tatcaattat tattaatttt tatgcgttat acggatagca gaaaactcac gcctgaaacg
     2401 gatgccaatc acaagaccgc ttccccgcag cccatccggc gaattaccag ccagacactg
     2461 ttaggtccgg atggcaaact gattatcgat catgacgggc aagaatatct gctccgtaaa
     2521 actcaggctg gcaagctgct gttaactaag tagcctttaa ctcgagcagc tgacttccag
     2581 ccgtttaccc cagtcaggtg gacgactgac atagtcatca tctctgtcgc tgaaaggatt
     2641 tcgcaacgcc tcatgcaggc ggtgcaattc catcatatca cccttttctg ccgcttcaat
     2701 cgcccgttgc gccaaccagt tgcgcaatac cagagctggg ttaacgcttt gtatcagttg
     2761 ctgacgctca ctatcgctaa cctcgtcttg ttgcaaacgc ccccgataac gggcaaacca
     2821 gtcatcaaat gccgcacgat caataaactc atcacgtagc ggtgacgccg cgctgtgctg
     2881 ctcggtcaga ctcagcatgc ggaatgtgcg ggtataatcg ctgcgctctc gcgccatcag
     2941 actgaataat tcattcagta gcgcgttatc ctctttttgc tccgtcatga agcccagttt
     3001 ctgccgcatc cgttgtccat aatgcgtcaa caaaacctgc tgatagctgt ccagggcctc
     3061 attcagggca tctacggcaa caaatggtga caatgtctgc gccagacgct gtaaattcca
     3121 caacgcgaca gcaggttgat tatcaaagct ataacgccct tgatgatccg agtgattaca
     3181 aataaaaccg ggttcgtaat catcaagaaa accaaacggc ccgtaatcaa gcgtcagtcc
     3241 cagcagcgac atgttgtcgg tattcatcac cccatgtgca aagccgaccg tctgccattg
     3301 ggcaattaac gacgcggtac gtgcgacaac atcgctaaac cagagacggt atttgtcctc
     3361 atcatcttca agatgtgacc agtaatgacg aatagcaaag tcagctaact gacgaacctt
     3421 ttccgactcg cggcggtagt aaaaatgttc gaaatgacca aagcgcagat gacttggtgc
     3481 cacacgcatc agcatcgcgc ctggctccgc cgtttcccga tacactggcg aatcgctggt
     3541 gacgatactt aacgcgcggg tcgtcggaat gcccagataa tgcatcgcct cactggcgag
     3601 actttctcgt atcgtcgaac gtaaaaccgc ccgtccatca cccattcgcg aataaggcgt
     3661 caggccagca cctttcagat gccagtccat tgtagtgcca tcagcaagca gttgttcgcc
     3721 gagtaaaatg ccgcgcccat cacccagttg gcccgcccag acgccgaact gatgaccact
     3781 gtaaacctgc gccagtggtg acataccagg cagtagggct tcgccgcccc agacacctgc
     3841 gccattttta aacagcgacg atggaatact cagcgtgtta gccagttcga tattatgcca
     3901 aatcagccgg gcattattta aaggcgtagg ggaaagtgct gtataggttt ctggcaattc
     3961 atcgcgccag cgggtaacaa aagacagggt catagatcct cctgtttgat agtgtagacg
     4021 gttactctcg tcttaaacac cagcaaacag aagggttatc gctgaaccaa cgtcgttatc
     4081 tgatttatgg gaacggcaga ccacaggcat ccctggagag catggaaatc gaagggggta
     4141 atctgtgcca atatttctgc cgtatcaatg cctcccgcaa tgatgcaatt gcaacaaggg
     4201 gaaatttgcg cctggatcgc acgaataaac ggttcgaagg agcggtgcgt gatttgttgc
     4261 tgaataaaac ttttatccag catgacgcgg gtgaaaagac catcaaaaac cgctttcatt
     4321 gtgctattgc ctgctcccaa attacctaac acaaggggat acacttgcga taaggataaa
     4381 agaccgcgat tatcttttcc ttcattgaga tgcgggtaat tctcattaat caacagttca
     4441 ataaacggat attttaatag ttcgcctgcg taattatccc tctctaataa taatgtagcc
     4501 acttgtggcg ttaaattaag ccaggcaaag agtttatgtt gaataaaaaa atgttggcat
     4561 gattttagta attccaactg ctctgaaaat aattgccaat gttgctcctc ggtaagttgc
     4621 gctataaccc gactcgtcgg gatacgcacc gtgccatctt cactggagaa atgggtaatt
     4681 aactcaacgc cgacaaggac ttgctggtta tctcttatcg gcaggaaata gcaatcagaa
     4741 tgataaagat tctccaaaaa aatcttcatg gtaaccgtcc ctctcgaagg atgtttttca
     4801 gtatccggct aaaagggatg aggccataag acataacaat tacagaagga gtaactttca
     4861 tttgttccat gttaaccact ttttcagggg tcttttttta gattatcctg attataaacg
     4921 aataatccat ttacggaatt tttgtctgca aaatactact gtattcagga gtaaatggac
     4981 ggcgatgtat catcgccgtc catttttcga catactagat tcgtctcgcc tgccagaaat
     5041 ttttctgcca atagacatta tcaagtgagg aacgcatcac tcccttgctg gtagaggcgt
     5101 ggataaattg gttgttggta tcgtaaatac ctacatgcaa accattttgt ccggaccccg
     5161 ttttgaaaaa gaccaggtca cctggcagca acttgtcttt atcaatttgc gtgccaatag
     5221 aggcttgttg tttggtttca cggggcagct gcaaatcgaa acgatcgcgc atcgtcacaa
     5281 ccacaaatcc cgaacagtcc acaccgcgcc gcgtcatgcc accataacga tacggcgtgc
     5341 catgccagct ttgtagctgg tcgttcaaac cggcaataac ggtaatcgaa tcagaaagtc
     5401 tggcatttgg cggcggtgct ttatggtggc tacacccggc cagaaacagt gctgtgatca
     5461 aaataaggca gaaacgcatt ccgtacggtt cctctgtttt ttattcttgc attaatttag
     5521 cgtcgtaatt acccgatttt caagatacta atgaaatcag atggtcaaaa tcagcattct
     5581 gtgaccttcg atatccagac ggcgaaaatt catcccatag gcctgcgcca gatttggcgg
     5641 cgtgagcacc tcttctctgc gtccactggc cagcattttt ccacctttta gcaaccacgc
     5701 ccgatgcgca tgacgcaacg tgtggttgag atcgtgactg ctcatcacaa tcgccagtcc
     5761 ttgctgacac agcgcgctca gaattttgtc taacgcactt tgttgcgcaa catcaagact
     5821 gttcatcggc tcatcaagaa gcagcaattg gcctgcggga ttggcttgtg gtgtgatttg
     5881 caacaccacc gcagcaagac ctacgcgttg ccattcaccg ccggaaagtt gattggtgct
     5941 acgaccgagt ttgtcatcaa gagccagcgc ccctgcgaca tcattcagta gttcggtacg
     6001 cgttttatcg tgctgatgca gtgtcaggta gtgccagacc ggcattgcaa acggcggcgt
     6061 ctgctgttgt gaaagatagg cgcgatgcag cgcgagtttt gttgcggacc atgcttccag
     6121 gggttgcccc gcgaactgaa tgcttccctt accgctggtc attccggcca ttcgtgcaag
     6181 caaggtactc ttacccgcgc cattcggccc caccaggtgc aggatctccc cagcccgaac
     6241 ctcgccagaa agtggcccca ggcgggtaga ttccgcaaca tcctgtaact gcatcacaat
     6301 agacattatt ttgccaacgc cagtttaatg ctttccatca caatggggtc ttccggcgtc
     6361 atatccgggg aaaaacgctg gatgaccaat ccgtccctgc caactaaaaa tttctcaaaa
     6421 ttccataaaa tatcatcagg gtacagcggt gcacgacctt tgctgaccat acgggcatag
     6481 aatccgctct cttccggcgc gactgcggtc ggcgctgcgg caatcaattt ttgatacagc
     6541 ggatggcgtc cttcgccgtt aacttcaatc ttactgaaca tcgggaacgt caccccccat
     6601 gtggtggtac agtaagtttt aatctcttca tcgctgcccg gttcttgttc cagaaactgg
     6661 ttgcacggga atcccagcac cacaaaacct cgatcggccc aggctttctg aatattctcc
     6721 aactgctcat attgcggcgt taagccacac tttgaggcga cattgacaat caacagcaca
     6781 ttaccggcgt acttctccag cgtggtcact tcaccgtcga tatctttcac tacggtcgtc
     6841 agaatggaat cttgcatcgt ttctcctggg tgtggtcagt aaaaatctta gcttttaatc
     6901 atagaccgtc tttttgcggc taacgtcttg cttttaacaa taaccagata aacaccggcg
     6961 cacccaacgt tgcggtgacc acgccaataa gcagctctgc ggcagctaat gccaggcgcg
     7021 ctacaatatc ggccagcagc aatgcgctcg cccctgccag cgcgcagccg ggaagtaata
     7081 cgcgatgatc ggttaaacca cacaaccgaa gaatatgtgg gatcaccaga ccaataaagc
     7141 cgatagcacc cgccagcgcc acactgacgc caaccatcca gccggtcgcg gccaccagca
     7201 cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc cgagatctcg ccaagtgcta
     7261 acatattcat cggcctggac tgacaacaga tccacaacaa cacggggatc aatgccagca
     7321 tcagccagct ttgccgccag tctacgccgc caaaaccgcc catcatccag tacatcagct
     7381 gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt cattagtgcg ctacagataa
     7441 tccctaatgc aacgccagcc agcaataacc gactggtcga aagatgacga cgggcgaaac
     7501 gtaagagtat taaagtaatg ataagcgcgc cagcaatcgc acacagccct agcgcccagt
     7561 tggggagttg cccttgccca agcaataccg cggcgataag ccccacgcct gcgccgttag
     7621 agacgccaag tagtccaggt tctgccagag gattttcaaa caacgcctgc attacagcgc
     7681 cggatatagc cagcgccgca ccaaccaaca atacagccag cgtacgtggc aggcgaattt
     7741 gccagacgaa cagttcgcca cgaggagtaa accagtcacc tagcgagatc cattgttcac
     7801 cggcgcaaag gcttaaaaga agcgccagca gcatcaaaac tgacaggcat aataaccagc
     7861 gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat ggtatccgtt ctgctgaagt
     7921 gtcatggcgt tgattttacg gtgactcttc gacagtgaaa agaaaaaagg ccgcagagcg
     7981 gccataaaca caagtaaaaa tcaacaagtt agataattat caaagactta cagacacaca
     8041 aaaacacagc caaccacaac aaataacagg gatgtggtca ctttgtggat catatcgccg
     8101 ccataaattt acttaataac agtaaccaca gccacaagca cggccatcgc agacaggaca
     8161 atacctgtga gtaaccaagt ctgatttgca aatgatttct gaagttcagt acgatgctca
     8221 gccatctcaa ctttcagaga ctggcgtaaa tcagccatct caagcctcaa cccttcgcga
     8281 atctccagta catcagattt ggtagcaaag gattcgcttc ttgtggtgag agtaatcagg
     8341 ttgtttttga tttcagtcac atcactttcc aggcaactga cccgccgttc aaggtcgtca
     8401 ttcatgccat cacctccatc attaccactt cctgttactt ggttgttgga tacattatag
     8461 gcattctctt tcttttttct gaaccgcgaa atatgtgcaa cattttctgc catcacttct
     8521 catcaccctg cttgcctacc cagttcataa aggcccgggc cgagtaacga tgaacaaacc
     8581 cacatctgtt acaggtaagt cggaattcat agttatggat attttcccga tcatgtccct
     8641 catatcccgg atacccagta aatggaccga tgtaatcaag cataacaaaa gtgcctaaat
     8701 cacactcctc agcagttttc ggctcaggcc ccacctcaac aatattcgtc aaatacatgt
     8761 acatatccgt atcgccacac gacaaacatt tttcattcgt agacgactca ttgagaaaac
     8821 gcgcaaaatt atcgagcgtt gccattcttt taagttcatc taaatgaaac ttcactctta
     8881 agcacctcct agtggattaa acctcaccgc atcctgcagg taatccggcg caagatgggc
     8941 ataaatcatc gttgtctgaa tctttgcgtg ccccagaatt ttctggagcg tcagaatatt
     9001 gccgccgttc atc
//
LOCUS       DADLTD010000169         8859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLTD010000169 DADLTD010000000
VERSION     DADLTD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.169"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..317
                     /gene="tnpA"
                     /locus_tag="JN701_003215"
                     /pseudo
     CDS             1..317
                     /gene="tnpA"
                     /locus_tag="JN701_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            377..451
                     /locus_tag="JN701_003216"
                     /pseudo
     CDS             377..451
                     /locus_tag="JN701_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(448..1771)
                     /locus_tag="JN701_003217"
                     /pseudo
     CDS             complement(448..1771)
                     /locus_tag="JN701_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1771..3387)
                     /locus_tag="JN701_003218"
     CDS             complement(1771..3387)
                     /locus_tag="JN701_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY7365568.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGHSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3683..4399
                     /locus_tag="JN701_003219"
     CDS             3683..4399
                     /locus_tag="JN701_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7365569.1"
                     /translation="MIYKSIAERLIIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4396..6066)
                     /locus_tag="JN701_003220"
     CDS             complement(4396..6066)
                     /locus_tag="JN701_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY7365570.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGSIGKLYWFMPPSANLALRELGIVLFLSVVG
                     LKSGGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMT
                     DPPALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6262..6690)
                     /gene="ibpB"
                     /locus_tag="JN701_003221"
     CDS             complement(6262..6690)
                     /gene="ibpB"
                     /locus_tag="JN701_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY7365571.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6802..7215)
                     /gene="ibpA"
                     /locus_tag="JN701_003222"
     CDS             complement(6802..7215)
                     /gene="ibpA"
                     /locus_tag="JN701_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY7365572.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7521..7853
                     /locus_tag="JN701_003223"
     CDS             7521..7853
                     /locus_tag="JN701_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7365573.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7855..>8859)
                     /locus_tag="JN701_003224"
     CDS             complement(7855..>8859)
                     /locus_tag="JN701_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY7365574.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRAYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
BASE COUNT         2258 a         2338 c         2218 g         2045 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttta aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtcctggct
      241 attttgccaa tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt
      361 ttacggcgca ccggataaat ttggttatgc gccgtcagcc gtgctaaaga atacggacca
      421 gcacccaacg gatgccagtc cacacaatta acgtaactct ggccagtacg ctttgttggc
      481 ctccagcaac tcatccagaa tagctttggc gaccgaggcg ctcggtaccg ttttcgataa
      541 cgtaatcgcc tgccacaggt gctgatatga acgctgttcc caggcgtcca cgaccagttt
      601 ttccaccgcc acttgctgac tcattaaccc tttctgaaac tgcgggatat cgccgaccac
      661 cagtggttct ggtccgttgt gtccgaccag acacgggatc tcgaccatcg cttcatcatc
      721 aaagttatgg attgccccgt tgttaggcac aatcagcaac atccgctcct gagtgttgaa
      781 ggcaattgcc gccgccagat cgacgatgta tgacgcatgt tcgtcaatct ccagcttggc
      841 cgccgctgaa tttcctgccg ctgtaatggc gcggcaagca tcgaaaacct gtttctcgcg
      901 atgttccatt cacctcattc gcgcgggtat gttcagggtt ggaatgctga acaacataat
      961 ccgggaagag ataatatttc agataggtgt tcggcaatgt atccgggtcc agtgcctgga
     1021 catcccgcgc ttttgcatag gtgtcgttcc agctcgcttc agtatgtgta tcttgctgcg
     1081 gtggaacata accgtgctta gagacatatt gccgcagctg gggcatcagg tcgttgccct
     1141 gcaaatcttc aattgatgtc caccagccaa agtggttcag gccgtagtag cgcacgcgca
     1201 tctgtttgcg atcttgcagc ccaacaattt gcgccatccg gctttcaata ccgattggca
     1261 tgtcacagat gttgaggatt ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa
     1321 ttgctgccgg gttggagtag ttgagcatcc aggcatttgg tgaatatttt tccatataat
     1381 ccaccagttc caggacgccg ccaatggaac gcatgccgta cgctattccg ccgggtccgc
     1441 aagtttcctg accaacaacg ccgtggcgca gcgggatttt ttcatccagt tcgcgcatcg
     1501 ggtatttgcc gacgcggatg tgcgccatga caaaatcaac gtcgctgaat gccacttcag
     1561 gatcggtggt gtaactaaac gcaatgtccg gcgctttctc tttaaggatg actttacagg
     1621 cttcggcaat cacttcctgc cgcgcaccat cgttatcata aaatttcagt gcacgaagcg
     1681 ggaaacggtc ctgattcgcc aggagcatca acacgatgcc tggcgtaaag gtgcttccac
     1741 cgcctgcgac aaccactgag aatttggtca ttatactgcc tccgtaatgg caacattttc
     1801 tgctgattga tgagaattaa ttaagctatc gagctgttca cgcagctggg atacatgcag
     1861 accaatgatc acctgaatgg catcgccact acggaagacg ccgtgcgctc ccagcttttt
     1921 aaagacttcg tcatccagcg tttgtgacat gtcatgcagt gcaatacgta aacgcgtcgc
     1981 gcaattgtta atgctggaga tattgccgac cccgcccagg gcttgcagga taccggcagc
     2041 ctgatccagc tctttttttg gctctgcagc ggtggtttgg cctcgcgagg ctttgtattc
     2101 ggcttttgag tagagtttca cttccgcatc ttcacgtccc ggcgtgcaca tgttgaattg
     2161 cagaatcagt gtgcggaaaa ccacgaagta cagcagggta aagcacaacc caatggcgat
     2221 ttgggtcagc atcatatccg cgtggttgct gaacatcggg atccagtttt gcggtaaaac
     2281 ctggtcaatc agacctccgc ccatgttgcc caccacacca aagagataca tcacggtcga
     2341 cattgaggcc gccagcacgg cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt
     2401 aaattccagc ggttcggtaa ttcccaccag catggcggtt aaggttgcgg gaatcagcaa
     2461 gcccgccact tttacccgat tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc
     2521 cacggcacca aagatttttg agtgaccgtg cagggcaaaa ccgccttccg ggaacaacga
     2581 tttcagcggc tcggcgctca gactgaactc ttgcagatgc tgcgcccagt acatctgaat
     2641 gccgccttca acagctgccg gaccaaagat aaactgtccg tagatgaagt ggtgtaaacc
     2701 ggttgggatc agaatacgtt cgaggaaggt gtaaacccac cccccaagcg cacccgccga
     2761 acgcaggaac gcttgcagag attcaatccc catttgtact tttggccaac cgagcaacgt
     2821 cagccaggca caggggatca tcaccaggaa ggcgataatc accacataag acgtcccctg
     2881 gaaaatgccg agaaaaaccg gcagtttttt atcgaacaga cggttatgca gcgccgtcac
     2941 aatgccggaa atgataattg cgccgataat gctggtatcg agggttttaa tcccggccat
     3001 cattgtcaga ccgctacctg ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc
     3061 ccagatcatt cccatcgcgt tgatgaaata gttccaggtc aggaaactca ccatcaccgc
     3121 cagacaagca cgcccctgcg cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat
     3181 cagcggcata ttacggaata ccgtccaacc gccctcttca ataatgtgta cgatttgcgc
     3241 gaataaactg ttcggatcgg tcagtgattc cccgacaaac atcgggtttt gcagcaagat
     3301 ggcaagaccc accacaatcc cggcgaaggg aaacagcagc actggcgtga acatcgcgcc
     3361 gccaaagcgt tgaatttgac tgagcatttc cacatcctct tttctcaatt ctgtaagtag
     3421 gaagcctgat tggttatgcg gcaggcctgg agcgaggata ggaagatgcg atcggcagaa
     3481 catgacgacc caatgggatt cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct
     3541 acgttttcgc gaaaaagtag acatctgtga gcactggtac acagcatcga acacatcttt
     3601 aaaaaaaaga tgttttttca atcgattaag cagaacttgt gggcgcatta cccgggcttg
     3661 caggcaaaaa agagatctag agatgatcta caaaagcatt gcggagcggt taataattcg
     3721 acttaattcc gcggatttca cgctaaacag ccttcttccc ggtgaaaaaa agttggcgga
     3781 agaatttgca gtatcccgga tgaccatccg taaagcgatt gacctgctgg tagcgtgggg
     3841 gctggtggtc cgccgccacg gcagcggcac ttacctggtg cgcaaagatg tgctgcatca
     3901 aaccgccagc ctgaccggac tggtggaggt gttaaaacgg cagggaaaaa cggtcaccag
     3961 ccaggtgctg atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat
     4021 tcaaatcaac gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct
     4081 gatgctggaa gacagctata tgccggttaa actgttccgt aatctttcgc tgcaacatct
     4141 ggaagggtcg aagtttgaat atattgaaca agagtgtggg attttgattg gcggtaatta
     4201 tgaaagcctg acgccggtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga
     4261 acacacgcca ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa
     4321 ttattcagtg atgttcagaa atgccagcga ataccaggtg gagtaccatt tacggcgact
     4381 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
     4441 aattcgcaga aacatcacta acggatagac agtggcgtaa gagagcgccg gcgcaccact
     4501 ggttggatga agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag
     4561 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
     4621 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt
     4681 gaccagggta ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac
     4741 gatccccagc tcccgcagcg cgaggttggc acttggcggc ataaaccagt acagcttgcc
     4801 gatactgccg atactgccga tacgcccgag gatcaacgcc ataatcagcg gaccgcccgc
     4861 cagccccagt ttcaacgcgg ccgggaaccc aggcacaaag acgggaatag aacctaacaa
     4921 tacgccaagc ccgatgccaa taaacactgg cagcatctga acctgttgca gtttttgctg
     4981 cgcattcccc agcacattgg caacggcatc aattgcggac ggacgcccca ccagattgag
     5041 gatatcgccg aactgcaggc tgatatcgcc actggcgacc agttcgaccc cggcacggtt
     5101 caggcgcgag ataacaacgt catagcgttc tttaaagtgc aggtcgcgaa tacgttttcc
     5161 gagcacgttt tcattggtga ccaccacacg ctcgacgcgc aaatcagtgc ctttcgtgga
     5221 cagcgaagta tcgacctcct gaccaatcac cagttgcgca ttatgtaaat ccgctggctg
     5281 ccccaccaga tgcagcaaat cgcccagttg gataatggta tctggcgaag gaacttttag
     5341 ggtttcttcg cgtttcagac gcgagcagat aattttgtcg ccgttgagaa tcggtacatc
     5401 tttaatagct aaatcatgca ggttagggtt ctcaacgcga atattgatag tcttgatcag
     5461 cgcgccgcca ttggtgcgtg aagactcgtg ctgctgagct tctgtctcga cattgacgcg
     5521 gaaaataacc cgcaacatcc acatggtgaa caaaatcccg caaatgccga atggatacgc
     5581 catcgcgtaa ctcatcccca tctgatcgac catttccatt ggtgtaccca ggtcgcgcaa
     5641 gatctgctgc cctgccccca gcgctggcgt attggtaacc gcaccggaga aaatccccag
     5701 cactaccggc agtggaatat caaacagttt atgcaggatg gcggtaacca gaccaccgat
     5761 gatgacgatc agaacagcaa acaggttgag gcgtaatccg gagacgcgca atgaggcaaa
     5821 gaagcccggc cctacttgaa tcccgatggt ataaacgaac aggatcaggc caaattcctg
     5881 aataacatgc agcatatcgc tacttaatgt catccccgcc tgagaaacaa aatggccgac
     5941 gatgatccca ccaaacagca cgccgccaat acctaatcct atgccgcgaa atttgacgtt
     6001 gccgataaac aaaccgacga ctgccaccaa agccagaata ctgaccgtta atgctatatc
     6061 actcatcgtc catttctctt gagataacat tttaattgct acggattatg gctgaatcgg
     6121 tgaagcagat ttgccttata gcgcacatta tcacgggaaa gaacgtgccg aaatatctta
     6181 aacagtcgaa atttaacgca ttgttgaaga agaaagacaa aaaaagcccc accagaatgg
     6241 cggggcaaag agaatagcta gttagctatt taacgcggga cgttcgctga tagcgatacg
     6301 ctgcgctgcg atgggttcag gctcattacg aattaaatca atatgcagta aaccgtttac
     6361 gaaggttgcg ccagagactt ccatattttc agccagcgta aagctcaggc taaatggctg
     6421 attcataagc ccttgatgca gccatttttt ctcttctttt ggctgctccg gcgtgccttt
     6481 tacgctcagg cgcgtacctt ccagttgaat ctctaaatct tcctgacgga aacctgccag
     6541 cgcaagggta atgcggtagt ggttatcgtc gcttttctca atgttgtacg gcgggaagct
     6601 ctggctttca ccggcgtttt gcagtgcgtt ggccagtttg tcaaaaccga tccattgacg
     6661 catcagtggg gataaatcga agttacgcat agtcatttct ccttctaaga agcgagtaag
     6721 tacctgcaaa tccgaagatt cgcatatgct ccctgacggc gagcatggag atgtcaggcc
     6781 gcgtcaggcg gccttaggga attagttgat ttcgatacgg cgcggttttt tcgcttccgg
     6841 aatcacgcgt tcgagatcga tatacagcaa accatttacc aggttagccc cacgaacatg
     6901 aatgttctca gctaactgga atttgcgttc aaagttgcgt tcagcgatgc cctggtacag
     6961 ataggtgcgc tctttttgtt cgtcggcgtg agcacctttc accaccagca gattatcctg
     7021 ggcggtaatt tccagttcgc tctcagcaaa accagccaca gcgatagcaa tgcggtaatg
     7081 gttttcgtct accagttcaa cgttatacgg agggtagccg ccattactct ggctctggtt
     7141 gttttctaag tggttaaaca aacggtcaaa tccaatagca gaacggtaaa gcggggataa
     7201 atcaaagtta cgcataatca atagctcctg aaatcagcga gaatgtaata ccttccacaa
     7261 tggacaggtc aggtagccag aacacccatc aggcgcgtcc tcatcggcta cgatgtaaaa
     7321 atgggtctgg aaatgaactt ttcaagcctt atcagcggac ttttttttgc agtttatggt
     7381 ctattgcata gactgagggg gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg
     7441 acgtaatgat gatgttattt tcagcaaggg atcgctataa ctcatcaggc caacacccac
     7501 gatcaacaga tgaatttatt atgataagaa atgtgttgtt agcgttcatg atatgcagcg
     7561 gaatgacatt actcggagga tgctccagcg tgatgtccca taccggaggg aaagaaggaa
     7621 cgtatcccgg cacccgcgcc agcgcaacca tgattggcga cgatgaaacc aactggggca
     7681 ctaagtcgct ggcgattctc gatatgccgt ttaccgctgt gatggacacg cttttactgc
     7741 cgtgggatgt gttccgcaaa gacagctcgg tgcgctcaca cgttgaaaaa agcgaagcca
     7801 acgcgcaagc caccaatgcg gttattccac cggccagaat gcctgataac tgatttagcg
     7861 atcagtttcg gtgatccaca gctggccgcc ttccatccac gccagccatt gaccatccgg
     7921 cgagaagacc acggcgtccg cagaaggtgg attggcgtgg ttttcggtta aatactcaac
     7981 ctcgccactt tgcgcatggg cacaagcaat tcgattatcc agcacaaagc ccaaccattc
     8041 tcctgacgga tgccagttaa atgcagactg aatatccgtt ttgttatggg ttaactggcg
     8101 cggctcgccg ccctgtggcg agataagcca cagttgcaca atgccgttat catcacgcat
     8161 taaaaacgcg atttgcgtac cctgcggatt acagcgcacc cagtggcgcg ggacgttgac
     8221 taaccccgga taagcccgat ggtgggtaaa ggttaaacgt cgctgcacga cgccgcgcgg
     8281 tggcgcgggc agggtggttt ccgttccact taacggcgca tcacctgccg ctttccagcc
     8341 agcttcatct tgcggtaact caacgataaa cagctccggc actttctcgc cctttggcga
     8401 aagtgtgtcg ccaataaacg ccagcgcgtg atttcctacc catccttctt cataagcacg
     8461 attgatttca tcgctgccag gctgcggcgt gggcgtggtt ttactcacca gcacgcacca
     8521 gtggctaccg ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggcgc
     8581 agcaacaccg acgtttcgca aatccagcgc cggatcgagt tcatgcatta catggtcgtt
     8641 ataggtaaag ctcacccttt caccgttcgg gctaaagaca tgcacatggc tgccgccgcg
     8701 cagcgctcct ggcgtatacg gcgcggtaat atccattgca tcgagattgc tcaccttgcc
     8761 gccttcagca atgactccgc gtcgatgatg gaaatcgtaa taccatgttt catcaggatt
     8821 ttccgggcca tggatgaaaa catatttctc tgacttcgg
//
LOCUS       DADLTD010000170         8733 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLTD010000170 DADLTD010000000
VERSION     DADLTD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.170"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            244..1155
                     /gene="ttdA"
                     /locus_tag="JN701_003225"
     CDS             244..1155
                     /gene="ttdA"
                     /locus_tag="JN701_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HAY7365575.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1152..1757
                     /gene="ttdB"
                     /locus_tag="JN701_003226"
     CDS             1152..1757
                     /gene="ttdB"
                     /locus_tag="JN701_003226"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY7365576.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1806..3268
                     /locus_tag="JN701_003227"
                     /pseudo
     CDS             1806..3268
                     /locus_tag="JN701_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
     gene            complement(3311..4324)
                     /gene="tsaD"
                     /locus_tag="JN701_003228"
     CDS             complement(3311..4324)
                     /gene="tsaD"
                     /locus_tag="JN701_003228"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY7365577.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4561..4776
                     /gene="rpsU"
                     /locus_tag="JN701_003229"
     CDS             4561..4776
                     /gene="rpsU"
                     /locus_tag="JN701_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY7365578.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4887..6632
                     /gene="dnaG"
                     /locus_tag="JN701_003230"
     CDS             4887..6632
                     /gene="dnaG"
                     /locus_tag="JN701_003230"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7365579.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6827..8668
                     /gene="rpoD"
                     /locus_tag="JN701_003231"
     CDS             6827..8668
                     /gene="rpoD"
                     /locus_tag="JN701_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY7365580.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2109 a         2330 c         2333 g         1961 t
ORIGIN      
        1 cctgcaagtc tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat
       61 aagcgcagtg tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta
      121 ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc
      181 acatttttag tggtagttta tggcggtgaa tttttccaga caaatacaaa aactggagtt
      241 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct
      301 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
      361 ctaaaggatg ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac
      421 atgcaaaaag cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc
      481 ttcgttaaag tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa
      541 gccgtggaag aggcaaccgt caaagcgcca ctacgtcaca atgcggtaga aatttttgac
      601 gaagtaaaca ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggacatcatc
      661 cccgacaatg acgatgcgga aatcgaagtt tacatggcag gtggcggctg cacgctacct
      721 ggccgctcga aagtgttaat gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc
      781 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc
      841 gccacctcgg tggaaaccgc cgccgtactc tcgcgtaaag ccattttgcg cccgattggc
      901 agccgccatc ccaatccaaa agcggcagaa ctggagctac gcctggaaga aggactcaac
      961 cgtctgggga ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtacatatc
     1021 gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg
     1081 catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
     1141 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
     1201 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc
     1261 accgccgctt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
     1321 tccacgctgg ccccatcgtg cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc
     1381 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga
     1441 aactggtggt tggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca
     1501 aggcgctaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtggaag
     1561 agattgaaga agtgcactgg acagagctcg gaatgccgga gtcgctgtgg gtctgccggg
     1621 gcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaat ctgatagccg
     1681 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg
     1741 tccattacat caaataaccc tcccggagag gctcgccccc tctccttttt cgcaggcata
     1801 acacgatgaa accttccact gaatggtggc gataccttgc gccgctggcg gtcatcgcca
     1861 ttattgctct aattccggtt cccgcagggc tggagagtca tacctggctc tactttgccg
     1921 tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga
     1981 tggtgggtat ctccatcatc gccatactct ctccctggct gctgttcagc cccgaacagc
     2041 tcgcccagcc aggctttaag ttcaccgcca aatccctctc ctgggcagtt tttggttttt
     2101 ctaattcggt tatctggctc attttcgccg cctttatgtt tggcacaggc tatgaaaaaa
     2161 ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt
     2221 ttctcggcta tgcggtgatg ttctccgagc tgatcctcgc acctgtaaca ccgtccaact
     2281 cggcgcgtgg tgcggggatt atctatccca tcatccgtaa cctgccaccg ctctatcaat
     2341 cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta catcatgtgg atggggattg
     2401 ttgccgactg cgtgaccagc gccattttct tgacggcgat ggcaccaaac ttgctgttaa
     2461 ttggactgat gaaaagcgca tctcacgcca cgctgagttg gggcgactgg ttcctcggga
     2521 tgttgccgct cagtattttg ctggtgctgc tggttccctg gctggcttat gtgctgtacc
     2581 cgccggtact gaagtctggc gatcaggtgc cgcgctggca gagacggaac tgcaggcaat
     2641 gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct
     2701 gtggattttc ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcact
     2761 gatgctgctt ctgcgcatta tcagttggga cgacattgtc agtaataaag cggcgtggaa
     2821 cgttttcttc tggctggcct cgcttatcac cctcgctacc ggactcaaca acactggttt
     2881 tattagctgg tttggcaaac tgttagcagg cagcttaagc ggttattcgc caacgatggt
     2941 gatggtggcg ttgattgtgg tgttttatct gctgcgctac tttttcgcca gcgccacggc
     3001 gtatacctcc gccctcgcac cgatgatgat tgccgctgcg ctggcgatgc cggaaatccc
     3061 gctgccggtg ttctgcctaa tggttggcgc ggcaattggt ctggggagca ttcttacacc
     3121 atacgccacc ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta
     3181 ctggcgactg ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt
     3241 actgtggatg cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca
     3301 cccaatagtt ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc
     3361 cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc
     3421 cgttatcagt acaaaactcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca
     3481 tttccgccag cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc
     3541 gcttaaagcc cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg
     3601 catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa
     3661 tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg
     3721 tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta
     3781 acggtccgcc aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg
     3841 cggcatcatc gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta
     3901 actgcgtatg accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt
     3961 ccagcatcgg cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc
     4021 aggcaaacgc cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag
     4081 ggcctgcggt ataggccaca gcatcaatgt cttttgccgt taagccagac tcctttagcg
     4141 ccgcctggat caacggtacg gttttgcgca cgtgatcgcg ggaggccagt tcaggcacga
     4201 cgccgccgta gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt
     4261 tttcatcgtc gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac
     4321 gcatgacttg ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt
     4381 aaaactttgt tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc
     4441 tttcaaaaag gccgcggtgc tttacaaagc agcagcaatt gcagtaaaat tccgcaccat
     4501 tttgaaataa gctggcgttg atgccagcgg caaaccgaat taatcaaagg tgagaggcac
     4561 atgccggtaa ttaaagtacg tgaaaacgag ccgttcgacg tagctctgcg tcgcttcaag
     4621 cgttcctgcg aaaaagcagg tgttctggcg gaagttcgtc gtcgtgagtt ctatgaaaaa
     4681 ccgactaccg aacgtaagcg cgctaaagct tctgcagtga aacgtcacgc gaagaaactg
     4741 gctcgcgaaa acgcacgccg cactcgtctg tactaattcc ccgagagcgt tgctctccga
     4801 tcagaccgag ttgtagttgt aaggccgtgc ttccgaaagg aatgcgcggc ttattttcgt
     4861 ttatgaattg ctaaaaatcg gggcctatgg ctggacgaat cccacgcgta ttcattaatg
     4921 atctactggc acgcactgac atcgtcgatc tgatcgatgc ccgtgtgaag ctgaaaaagc
     4981 agggcaagaa tttccacgcg tgttgtccat tccacaacga gaaaaccccg tcattcaccg
     5041 ttaacggtga gaaacagttt taccactgct ttggatgtgg cgcgcacggc aacgcgatcg
     5101 acttcctgat gaactacgac aagcttgagt tcgtcgaaac ggtcgaagag ctggcagcaa
     5161 tgcacaatct tgaagtgcca tttgaagcag gcagcggccc cagccagatc gagcgccatc
     5221 agaggcaaac gctttatcag ttgatggacg gtctgaatac gttttaccaa caatctttac
     5281 aacaacctgt tgccacgtct gcgcgccagt atctggaaaa acgcggatta agccacgagg
     5341 ttatcgctcg ctttgcgatt ggttttgcgc cccccggctg ggacaacgtc ctgaagcggt
     5401 ttggcggcaa tccagaaaat cgccagtcat tgattgatgc ggggatgttg gtcactaacg
     5461 atcagggacg cagttacgat cgtttccgcg agcgggtgat gttccccatt cgcgataaac
     5521 gcggtcgggt gattggtttt ggcgggcgcg tgctgggcaa cgataccccc aaatacctga
     5581 actcgccgga aacagacatt ttccataaag gccgccagct ttacggtctt tatgaagcgc
     5641 agcaggataa cgctgaaccc aatcgtctgc ttgtggtcga aggctatatg gacgtggtgg
     5701 cgctggcgca atacggcatt aattacgccg ttgcgtcgtt aggtacgtca accaccgccg
     5761 atcacataca actgttgttc cgcgcgacca acaatgtcat ttgctgttat gacggcgacc
     5821 gtgcaggccg tgatgccgca tggcgagcgc tggaaacggc gctgccttac atgacagacg
     5881 gccgtcagct acgctttatg tttttgccgg atggcgaaga ccctgacacg ctggtacgaa
     5941 aagaaggtaa agaagcgttt gaagcgcgga tggagcaggc gatgccactc tccgcatttc
     6001 tgtttaacag cctgatgccg caagttgatc tgagtacccc tgacgggcgc gcacgtttga
     6061 gtacgctggc actgccattg atatcgcaag tgccgggcga aacgctgcga atatatcttc
     6121 gtcaggaatt aggcaacaaa ttaggcatac ttgatgacag ccagcttgaa cgattaatgc
     6181 caaaagcggc agagagcggc gtttctcgcc ctgttccgca gctaaaacgc acgaccatgc
     6241 gtatacttat agggttgctg gtgcaaaatc cagaattagc gacgttggtc ccgccgcttg
     6301 agaatctgga tgaaaataag ctccctggac ttggcttatt cagagaactg gtcaacactt
     6361 gtctctccca gccaggtctg accaccgggc aacttttaga gcactatcgt ggtacaaata
     6421 atgctgccac ccttgaaaaa ctgtcgatgt gggacgatat agcagataag aatattgctg
     6481 agcaaacctt caccgactca ctcaaccata tgtttgattc gctgcttgaa ctgcgccagg
     6541 aagagttaat cgctcgtgag cgcacgcatg gtttaagcaa cgaagaacgc ctggagctct
     6601 ggacattaaa ccaggagctg gcgaaaaagt gatttaacgg cttaagtgcc gaagagcgat
     6661 cgggaagccc ccgacagccg cactgagagg cagcggcaaa tatataagta cgccctcgta
     6721 attatcgttg gcggtaaaca accgttggat ttcagcgtta acggctgaag gacatcgggt
     6781 caatcgccca acaccaacct catgaaataa gtgtggatac cgtcttatgg agcaaaaccc
     6841 gcagtcacag ctgaaacttc ttgtcacccg tggtaaggag caaggctatc tgacctatgc
     6901 cgaggtcaat gaccatctgc cggaagatat cgtcgattca gatcagatcg aagacatcat
     6961 ccaaatgatc aacgacatgg gcattcaggt gatggaagaa gcaccggatg ccgatgatct
     7021 gatgctggct gaaaacaccg cggacgaaga tgctgccgaa gccgccgcgc aggtgctttc
     7081 cagcgtggaa tctgaaatcg ggcgcacgac tgacccggta cgcatgtaca tgcgtgaaat
     7141 gggcaccgtt gaactgttga cccgcgaagg cgaaattgac atcgctaagc gtattgaaga
     7201 cgggatcaac caggttcaat gctccgttgc tgaatatccg gaagcgatca cctatctgct
     7261 ggaacagtac gatcgtgttg aagcagaaga agcgcgtctg tccgatctga tcaccggctt
     7321 tgttgacccg aacgcagaag aagatctggc acctaccgcc actcacgtcg gttctgagct
     7381 ttcccaggaa gatctggacg atgacgaaga tgaagacgaa gaagatggcg atgacgacag
     7441 cgccgatgat gacaacagca tcgacccgga actggctcgc gaaaaatttg cggaactacg
     7501 cgctcagtac gttgtaacgc gtgacaccat caaagcgaaa ggtcgcagtc acgctgccgc
     7561 tcaggaagag atcctgaaac tgtctgaagt attcaaacag ttccgcctgg tgccgaagca
     7621 gtttgactac ctggtcaaca gcatgcgcgt catgatggac cgcgttcgta cgcaagaacg
     7681 tctgatcatg aagctctgcg ttgagcagtg caaaatgccg aagaaaaact tcattaccct
     7741 gtttaccggc aacgaaacca gcgatacctg gttcaacgcg gcaattgcga tgaacaagcc
     7801 gtggtcggaa aaactgcacg atgtctctga agaagtgcat cgcgccctgc agaaactgca
     7861 gcagattgaa gaagaaaccg gcctgaccat cgagcaggtt aaagatatca accgtcgtat
     7921 gtccatcggt gaagcgaaag cccgccgtgc gaagaaagag atggttgaag cgaacttacg
     7981 tctggttatt tctatcgcca agaaatacac caaccgtggc ttgcagttcc ttgacctgat
     8041 tcaggaaggc aacatcggtc tgatgaaagc ggttgataaa ttcgaatacc gccgtggtta
     8101 caagttctcc acctacgcaa cctggtggat ccgtcaggcg atcacccgtt ctatcgcgga
     8161 tcaggcgcgc accatccgta ttccggtgca tatgattgag actatcaaca aactcaaccg
     8221 tatttctcgc cagatgctgc aagagatggg ccgtgaaccg acgccggaag aactggctga
     8281 acgtatgctg atgccggaag acaagatccg caaagtgctg aagatcgcca aagagccaat
     8341 ctccatggaa acgccgatcg gtgatgatga agattcgcat ctgggggatt tcatcgagga
     8401 taccaccctc gagctgccgc tggattctgc gaccaccgaa agcctgcgtg cggcaacgca
     8461 cgacgtgctg gctggcctga ccgcgcgtga agcgaaagtt ctgcgtatgc gtttcggtat
     8521 cgatatgaac accgaccaca cgctggaaga agtgggtaaa cagttcgacg ttacccgcga
     8581 acgtatccgt cagatcgaag cgaaggcgct gcgcaaactg cgtcacccga gccgttctga
     8641 agtgttgcgt agcttcctgg acgattaatc ggtaggccgg atcaggcgtt acgccgcacc
     8701 cggcactagt ccctctgcac aaacgccacc ttt
//
LOCUS       DADLTD010000171         8719 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLTD010000171 DADLTD010000000
VERSION     DADLTD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.171"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            132..197
                     /locus_tag="JN701_003232"
     CDS             132..197
                     /locus_tag="JN701_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365581.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            336..947
                     /gene="ruvA"
                     /locus_tag="JN701_003233"
     CDS             336..947
                     /gene="ruvA"
                     /locus_tag="JN701_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY7365582.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            956..1966
                     /gene="ruvB"
                     /locus_tag="JN701_003234"
     CDS             956..1966
                     /gene="ruvB"
                     /locus_tag="JN701_003234"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY7365583.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2016..2801)
                     /gene="znuB"
                     /locus_tag="JN701_003235"
     CDS             complement(2016..2801)
                     /gene="znuB"
                     /locus_tag="JN701_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY7365584.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2798..3553)
                     /gene="znuC"
                     /locus_tag="JN701_003236"
     CDS             complement(2798..3553)
                     /gene="znuC"
                     /locus_tag="JN701_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY7365585.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3632..4564
                     /gene="znuA"
                     /locus_tag="JN701_003237"
     CDS             3632..4564
                     /gene="znuA"
                     /locus_tag="JN701_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY7365586.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4580..5902
                     /gene="mepM"
                     /locus_tag="JN701_003238"
     CDS             4580..5902
                     /gene="mepM"
                     /locus_tag="JN701_003238"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY7365587.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6022..6993
                     /gene="lpxM"
                     /locus_tag="JN701_003239"
                     /gene_synonym="msbB"
     CDS             6022..6993
                     /gene="lpxM"
                     /locus_tag="JN701_003239"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7365588.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(7082..>8719)
                     /locus_tag="JN701_003240"
     CDS             complement(7082..>8719)
                     /locus_tag="JN701_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY7365589.1"
                     /translation="DGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTSE
                     SATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCISR
                     VYLETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININN
                     NWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGKE
                     YITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYH
                     YNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDR
                     PTHSDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLELTLLRSQHKP
                     GEIVKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPVK
                     PHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYHY
                     VQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2373 a         2013 c         2127 g         2206 t
ORIGIN      
        1 cacctataca tgtaaagacg atatctccaa tttcatattc agcagggtaa ttaatattca
       61 cgttgtcatt ccattgaaat agatacgaat atacgtaata gcataaaaaa attatttttt
      121 caggaggtgc aatgtatatt tttattacgc atttcttcac tgaatacgta atatttaaat
      181 atttgcttcc aatataacct gtagaataaa tcatactgcg ccatttttca gttcatcgag
      241 acacctcgca agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac
      301 agcattatct ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca
      361 tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc
      421 atatgccgat gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca
      481 cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc
      541 gcacattgtt caaagagttg atcaaaacca acggcgtcgg cccgaagttg gcgctggcga
      601 tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt tgaacgtgaa gaagtggggg
      661 cactggtgaa actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga
      721 aagaccgatt taaaggtttg catggcgatc tgtttacccc tgccgccgac ctggtactca
      781 catcacctgc cagcccggcg accgacgatg ctgaacagga agcggttgcc gcgctggtgg
      841 cgctgggcta taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgacg
      901 ccagcagtga aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat
      961 tgaagcagac cgtctgattt ctgccggtac cactttgccg gaagatgtag cagatcgcgc
     1021 cattcgccct aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga
     1081 gattttcatc aaagcagcga aactgcgcgg cgatgccctc gatcatttgt tgatttttgg
     1141 tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa
     1201 tttacgcacg acttctggtc cggtgctgga aaaggcgggc gatctggcag cgatgctcac
     1261 taaccttgaa ccgcatgacg tgctgtttat tgatgagatc caccgtttat cgccagttgt
     1321 tgaagaagtg ctgtacccgg caatggaaga ctaccaactg gatatcatga ttggtgaagg
     1381 tccggcggca cgctccatta aaattgattt gccgccgttt accctgattg gcgcaaccac
     1441 gcgcgcaggt tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc aacgtctgga
     1501 gttttatcag gtgccggatc tgcaatatat cgttagtcgc agtgcacgct ttatggggct
     1561 tgagatgagt gatgacggcg cgctggaagt tgctcgtcgc gctcgcggta cgccacgtat
     1621 tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat
     1681 ctcggcagat atcgctgctc aggcgctgga catgttgaat gtcgatgctg aaggtttcga
     1741 ttatatggac cgcaaattgt tgctggcggt aatcgataag ttctttggtg ggccggtagg
     1801 tctggataac ctggcggcgg ctattggcga agaacgtgaa accattgagg atgtgctgga
     1861 accttatttg attcagcaag gctttttgca gcgtacaccg cgtgggcgta tggcgacgac
     1921 gcgggcgtgg aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc
     1981 tcgcgccgca tccgacaaat gtgttcagcg atagattagc tggcctgctt tttcatcata
     2041 ctgataataa ataacagtgc tgcacatagc accaccgacg gacccgccgg cgtatcgtaa
     2101 aatgcggaaa aggttaaacc gcccgttacg gcaaccatcc ccaccaacac agcgacacct
     2161 gccatctgtt ccggcgtgcg ggcaaagcga cgtgcagtag cagcaggaat aatcagcagt
     2221 gaagtaataa tcaacgcgcc aacaaatttc atcgctacac caatcgtcaa tgccgtcacc
     2281 agcatcaaca acaatttcac gcgctgtaat ttcacaccat caacaaacgc cagatccggg
     2341 ctaatcgtca tcgacagcaa attgcgccat tgccagaaca aaatagccac cacgatgacc
     2401 acgccaatcg caatagcgat gagatcttct ggcgtcactg ccagcaaatc accgaacagg
     2461 taagccatca aatcaacacg aatgttagac atcagactaa cgaccaccag gcccagcgac
     2521 agggcactgt gcgccataat ccctaataac gtgtcaagcg ccagctgtgg acgtttctcc
     2581 agccatacca gaccgcccgc caggagcagc gtaacggcaa ttaccgcata gaatggattc
     2641 acgtccagca acaaaccaaa cgcgacgcca agtaatgagg catgagccag cgtatcaccg
     2701 aaataagaca tacgacgcca gactacaaac gaacccagcg gacccgcggc acaggcgagc
     2761 atgatcccgg ctaaccaacc gggaaataat aattcaatca tgagcgatca tttccccgac
     2821 gcaaaacaat tcgtccctgt aaatcgtgac gatgattatg atgatggcga tagataccca
     2881 gttgttcagc accacgagga ccaaacatag aaataaactc cggatgtagg gaaacaactt
     2941 ccggtgtgcc ggaacaacaa atgtggtgat tcagacacag cacttcatcg gtttttgcca
     3001 ttaccagatg cagatcgtga gagaccatta aaacgccaca atccagttcg cgacgcaact
     3061 ggtcaataag gtcatataac gccacctggc catttacatc cacgccttga gtgggttcat
     3121 ccagcactaa taattgcggt cgatttaaca atgctcgcgc taacagtaca cgctgtgttt
     3181 cgccaccaga gagcttttgc atcggtgcgt taatcagatg cccggcctgg acacgtttca
     3241 gtgcaggcaa aatatcttct ttatgtgtgc cagggcgtaa gcgtaaaaaa cggtttacgg
     3301 tcagtggcaa cgtggtgtcg agatacagct tctgcggtac atagccgatg cgtagttttc
     3361 cgttgcgctt gataacccct tcatcgggtg ttaccagccc gagcactacc cgtaccagtg
     3421 tcgacttacc tgcgccattt ggcccaagta aagtcaaaat ttttccaggt ttaagttcca
     3481 gcgacacatc agagaggacg cggcgttggc caaaagaaac cgagacattt tccagggaaa
     3541 ccagacttgt catgttaatt ttagtcttgc agtagtcatg agatgttata atatcacact
     3601 tctcatattc attacgatga ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc
     3661 agcattatcc gccgctctct ggggaggtgc aacacaggcc gcagatgccg ccgttgtcgc
     3721 ttcgcttaaa cccgttgggt tcatcgcttc tgccattgct gatggtgtaa cagaaacgga
     3781 ggttttactg cctgacggcg cttcagaaca tgattattca ctgcgtccat cggatgtaaa
     3841 acgcttacag aacgcggact tagtcgtttg ggttggcccg gagatggaag cgtttatgca
     3901 aaaaccggta agcaaattac taggagcgaa gcaggtaacg attgcgcagc ttgaagatgt
     3961 gaaactgcta ctgatgaaaa gtattcacgg cgatgatgat gatcacgacc acgcggaaaa
     4021 aagtgaagaa gatcaccatc acggcgattt caacatgcat ctttggcttt ccccagagat
     4081 agcgcgggct acagcggttg caatccatgg aaaattagtg gaacttatgc cgcaaagtcg
     4141 agccaaactt gacgccaacc tgaaggattt tgaggcacaa ttagcctcaa ccgaaacgca
     4201 ggttggtaac gagctcgcgc cgctcaaggg gaaaggttat ttcgtttttc acgatgctta
     4261 cggctatttc gaaaaacagt tcggactgac accgcttggt cattttaccg ttaaccctga
     4321 gattcaacct ggcgcgcagc gtttacatga aataagaaca cagttggttg agcaaaaagc
     4381 aacctgcgtt tttgctgagc cacagttcag gccagcggtc gttgagagcg tcgcacgagg
     4441 gacatccgtt cgtatgggaa cgttggatcc cttggggacg aatatcaaac tgggtaaaac
     4501 aagctattca gaattcctga gtcaattagc caaccagtat gcgagctgcc tgaaaggaga
     4561 ttaatgagga agtgattacg tgcaacagat agcccgctct gtcgccctgg cgtttaataa
     4621 tttaccgcga ccacaccgcg ttatgttggg gtcgctcacc gttcttactc tggccgtcgc
     4681 tgtctggcgg ccctatgttt atcaccgcga tgccacgcca attgtcaaaa ccattgagct
     4741 ggaacagaac gaaattcgtt cgctcttacc tgaagccagt gagccgattg atcaagctgc
     4801 acaagaagat gaagccattc cccaggacga actggatgac aaaatcgccg gtgaagcggg
     4861 cgtgcatgaa tatgttgttt ccactggcga tacgctaagc agcattctca atcagtatgg
     4921 tattgatatg ggtgatatca cccaactggc tgcggccgac aaagaattgc gtaacctgaa
     4981 aatcggtcaa caactctcct ggacattaac cgcggacggc gaactgcagc gcctcacctg
     5041 ggaagtgtct cgtcgtgaaa cccgaaccta tgaccgtact gccgctaacg gttttaaaat
     5101 gaccagcgaa atgcagcaag gagagtgggt taacaatctg ctgaaaggta ccgtcggagg
     5161 gagctttgtt gccagcgcca gaaacgccgg tttaaccagc gccgaagtga gcgcagtgat
     5221 taaagccatg cagtggcaaa tggatttccg caaactgaaa aaaggcgatg aatttgcggt
     5281 gttaatgtct cgagagatgc ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg
     5341 tttgcgttca gaaggtaaag attattacgc aatccgcgct gaggatggca aattctacga
     5401 ccgtaacggt actggtctgg cgaaaggatt cttgcgattc ccgacggcga aacagttccg
     5461 tatctcgtct aactttaacc cgcgtcgtac taatccggtg accggtcgcg ttgcaccaca
     5521 cagaggtgtt gatttcgcca tgccgcaggg tacgccagtg ctttcagtgg gtgacggtga
     5581 agtggtggtt gccaaacgca gtggcgcagc aggttattat gtggctattc gtcatggtcg
     5641 cagctacacc acgcgttata tgcacttgcg caagattctg gtgaaaccgg gacagaaggt
     5701 gaaacgtggc gaccgtatcg cgctttccgg taataccgga cgttcaaccg ggccgcatct
     5761 gcactatgaa gtatggataa accagcaggc cgtaaacccg ctgacggcaa aactgccgcg
     5821 taccgaaggg ctgaccggct ccgatcgtcg cgaattcctg gcgcaggcca aagagattgt
     5881 gccgcagcta cggtttgatt aattaacatc cattcgcagc cggtacgcag tcagtaccgg
     5941 ctttttttat ttggtgcggg gcaagttgcg ccgctacact atcaccagat tgatttttgc
     6001 cttatccgaa actggaaaag catggaaacg aaaaaaaata atagcgaata cattcctgag
     6061 tttgataaat cctttcgcca cccgcgctac tggggagcat ggctgggcgt agcagcgatg
     6121 gtgggtatcg ctttaacgcc gccaaagttc cgtgatccca ttctggcacg gctgggacgt
     6181 tttgccggac gactgggaaa aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc
     6241 tttccagaac gtagtgaagc tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg
     6301 ccgcaagcga tggcaatgat ggctgagttg gcaatacgcg gaccggagaa aattcagccg
     6361 cgcgttgact ggcaagggct gcagatcatc gaagagatga ggcgtaataa cgagaaagtt
     6421 atctttctgg tgccgcacgg ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa
     6481 gggcagaaaa tggcagcgat gttccataat cagggcaacc cggtttttga ttatgtctgg
     6541 aacacggtgc gtcgtcgctt tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca
     6601 ttcatccagt cggtacgtca ggggtactgg ggatattatt tacccgatca ggatcatggc
     6661 ccagagcaca gcgaatttgt ggatttcttt gccacctata aagcgacgtt gcccgcgatt
     6721 ggtcgtttga tgaaagtgtg ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc
     6781 aagacgcatc gtctgacgat tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat
     6841 gatcatacga ttgcgcggcg gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca
     6901 gaacaataca cctggatact aaaattgctg aaaactcgca gaccgggcga aatccaaccg
     6961 tataagcgta aagatcttta tcctatcaaa taaaaaaaag cctctcaaat tattgagagg
     7021 ctgtactcga taaaaaatct caatgagtgt tcccactatt ttgagttttt aggttagtac
     7081 gctattgagt taatcgtagc gtgataattt caaaaggttt gaactcaaga ttgcaatatt
     7141 gattgcaata aaaactgtca tctaacttat tttcttgaag atcgacatat tgaacgtagt
     7201 gataattaca tacccacgaa agcatggctg aaatttctaa tccatgactc tcatataatc
     7261 ttacgattat accgtcgccg tcttcagcta attttattgt ttcgatgata acgctgttag
     7321 tatcgatagc aaaaaatgat agtgaagggg gtaattcacc cgggtgcggt ttgacaggga
     7381 cgacctgtaa gggatgattc attatatagg cttgattaat aacaccaccc acacggtaat
     7441 ctccatgatg aggaaataag gagaatatga atttttgttt accaatatct gcaaaattat
     7501 taatcaggaa attatcataa ttgtctgtct taacaatttc cccaggttta tgctgcgaac
     7561 gcagcaaagt taattcaagc gtctggtctt tgacacgata gccatatttg ttttttgcta
     7621 ttaaagctat gccagtttct cgatcagaaa ggtcgaccca gtgatgtgct ggtatttcat
     7681 ctttggcaaa agcgaacgaa tcatcgctat gcgttggtct gtcaattaca ccgaactgaa
     7741 tttgacattg tgcaagagat gtttgtactg acaccgggaa tttgactttg agagaaacat
     7801 taggttcgtg ccaatcaata aaagtttgga catcaacgcg tggactgtcc tgtgtcaggg
     7861 taaatgcgaa gcgaagataa gaggaattgt agtgataaat cagcgtcatc gtagttttag
     7921 gcccgctgat tacagttgtc attttttcag cgagtaaaac atatgattcc ccatcacgat
     7981 aatttgaagc aaaatcccag gccgcgaaaa aacctgcatc ttcgtggtat gcacggatct
     8041 cactgtgcat attttcagta atgtattctt taccatatct tttatcatat agcgaaatta
     8101 aatcaccgtt ttctgaaaaa agaagtttaa tgtaattatt ttcaatactt cgagattctg
     8161 ccttcagacc atttacgatc ttattctttg gatcaatcac agcataacca taagagttta
     8221 ctcgtgcttt gagccagttg ttgtttatat tgatccattc gttacgagca aaacaggtgg
     8281 tattaaataa aatgtgcgga tctttataag aagaagtatc aattttactt aataaagtag
     8341 actgcgtatc agaaataatt ttctcggttt ttgcttctag tttaatatat tccttttcag
     8401 tttcaagata aactcttgaa atacatgatc cgggaaggat gtcatggaat tgcaaggtca
     8461 gcatctcttt ccatatttca tcaaactcac tgcgaagaat tgatgtcata ttattagtaa
     8521 tagttgtaaa aaattcagca tcatgtaatt tattttccat atttcgatta tgagctttag
     8581 ttgctgactc tgatgtaaag cacccttgat gagcctcgaa ataaagttca cctgaaataa
     8641 tgggcaagct ttcgcgaaaa gcgtcggcgt aagtgaagaa cttatcaaca cggctgaaat
     8701 caacatgagg taagccatc
//
LOCUS       DADLTD010000172         8598 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLTD010000172 DADLTD010000000
VERSION     DADLTD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.172"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..183
                     /locus_tag="JN701_003241"
     CDS             <1..183
                     /locus_tag="JN701_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY7365590.1"
                     /translation="EAIKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQ
                     KGEVVGVDDQCKEHKE"
     gene            402..644
                     /locus_tag="JN701_003242"
     CDS             402..644
                     /locus_tag="JN701_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365591.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="JN701_003243"
     CDS             complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="JN701_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY7365592.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="JN701_003244"
     CDS             complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="JN701_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7365593.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2532..3434)
                     /gene="fdxH"
                     /locus_tag="JN701_003245"
     CDS             complement(2532..3434)
                     /gene="fdxH"
                     /locus_tag="JN701_003245"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7365594.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="JN701_003246"
     CDS             complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="JN701_003246"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5910..5912),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7365595.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6691..7524
                     /gene="fdhD"
                     /locus_tag="JN701_003247"
     CDS             6691..7524
                     /gene="fdhD"
                     /locus_tag="JN701_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY7365596.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7678..>8598
                     /locus_tag="JN701_003248"
     CDS             7678..>8598
                     /locus_tag="JN701_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HAY7365597.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPY"
BASE COUNT         2048 a         2175 c         2274 g         2101 t
ORIGIN      
        1 gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg atctgcaatt
       61 ttacgcgaag cggttgagca atatctggag cggcagcaat ttccggtgcg taaagcaaag
      121 ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga gcataaggaa
      181 tgaaaaaaat ttttcattct tgaatataaa aaaacagatg cccttattct ggtattaata
      241 caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc tggaaaacgc
      301 ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact
      361 tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta
      421 tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc
      481 tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc
      541 tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg
      601 aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt
      661 caatcagatt gctgacaacg tgcgcgttgt tcatgctgga tgcggcgtga acgccttatc
      721 cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc
      781 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
      841 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
      901 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      961 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
     1021 catagccttc ttgctccatt cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg
     1081 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
     1141 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     1201 tctgttcaca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
     1261 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     1321 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
     1381 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
     1441 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     1501 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     1561 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     1621 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
     1681 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     1741 gcaggtaatc atccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     1801 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1861 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1921 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1981 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     2041 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     2101 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     2161 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     2221 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     2281 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     2341 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     2401 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgctaggat
     2461 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     2521 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     2581 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     2641 aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     2701 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     2761 ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg
     2821 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2881 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     2941 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     3001 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     3061 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag acagcctgga
     3121 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     3181 acttccgaga agcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     3241 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     3301 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     3361 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     3421 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     3481 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt ggcaataaag
     3541 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     3601 ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag
     3661 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     3721 gactccccga tttccacaaa ctgctctggc tgcaaaatcg cgttcaacag cgcgtgtttg
     3781 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     3841 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     3901 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     3961 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     4021 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     4081 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     4141 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     4201 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     4261 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     4321 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     4381 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     4441 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     4501 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     4561 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     4621 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     4681 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     4741 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     4801 ttgttcggga atgaggcaac agggttaaaa ccctggcaga tatagccatt gaccttgccc
     4861 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     4921 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     4981 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     5041 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     5101 cctggcaggc tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     5161 cgcagggcgt taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc
     5221 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     5281 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     5341 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     5401 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     5461 ccgttttcgt ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca
     5521 tagccggtga aaaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg
     5581 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     5641 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtt agctaccgcc
     5701 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     5761 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     5821 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     5881 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     5941 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     6001 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     6061 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     6121 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     6181 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     6241 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     6301 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     6361 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     6421 aaacctagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     6481 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     6541 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     6601 cagaattggc tcgatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     6661 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa
     6721 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     6781 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     6841 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     6901 ctttccgagg ggattatcga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     6961 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     7021 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     7081 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     7141 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     7201 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     7261 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     7321 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     7381 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     7441 gagcgctgta atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc
     7501 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
     7561 acttattcat ttcgtgaatt ttattatttt tatttataaa taaaacgcgt gtagtggcgt
     7621 tttctttttt catttattat gttcactctc ttatctatat atttataagg caattaaatg
     7681 aaaaggaatt tattatcttc cgctattata gtcgccatca tggccctcgg tctgacgggt
     7741 tgtgatgata aaaaagccga aacagaaacg cccccgcctg ccaatagtca acctgccgca
     7801 ccagctcctg aagctcccgt tgcaaaagca gaagctaaac ctgaaacacc tgcgcaaccg
     7861 gtggtcgatg aacaagcggt tttcgacgaa aaaatggatg tctatatcaa gtgctataac
     7921 aagttacaga tccctgtaca gcgcagtctg gcgcgttatg ttgactggct gaaagatttt
     7981 aaacaggggc ctaccggtga agagcgtact gtttatggca tctacggcat tagtgaatcc
     8041 aacctcgctg agtgtgaaaa aggcgtaaaa agtgctgtgg cgttaacgcc tgcgctgcaa
     8101 ccaattgatg gcgtggcggt gagttatatc gatgctgccg tggcgttggg taataccatt
     8161 aacgaaatgg ataaatatta cacccaggaa aattataaag acgatgcgtt tgcgaaaggg
     8221 aaaacgctgc accagacatt cttaaaaaat ctggaagcct ttgaacctgt agcagaatct
     8281 tatcatgcgg cgattcagga aattaacgat aagcgccagc ttgcggaact gaaaaatatt
     8341 gaagaaagag aagggaaaat attccactac tattctctgg cagtcatgat ttcagcgaaa
     8401 caaattaata acctgatatc gcaagagaag tttgatgttg atgcggcaat gaagaaagtg
     8461 tctgaactgg aaacgctggt ggcgcaggcc aaagaagcgg ataaaggcgg catgaatttc
     8521 tcgtttatta attcggcagg ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc
     8581 agagataaag tcccgtac
//
LOCUS       DADLTD010000173         8514 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLTD010000173 DADLTD010000000
VERSION     DADLTD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.174"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..257
                     /gene="csgC"
                     /locus_tag="JN701_003249"
     CDS             <1..257
                     /gene="csgC"
                     /locus_tag="JN701_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY7365598.1"
                     /translation="IIPEVTLTQSCLCRVQILSLREGGSGQSQTKQEKTLSLPANQPI
                     ALTKLSLNISPEDRVKIVVTVSDGQALHLSQQWPPSSEKS"
     gene            378..689
                     /locus_tag="JN701_003250"
     CDS             378..689
                     /locus_tag="JN701_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7365599.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            785..1318
                     /gene="ymdB"
                     /locus_tag="JN701_003251"
     CDS             785..1318
                     /gene="ymdB"
                     /locus_tag="JN701_003251"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY7365600.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1260..2741
                     /locus_tag="JN701_003252"
     CDS             1260..2741
                     /locus_tag="JN701_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7365601.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2749..3906)
                     /gene="mdoC"
                     /locus_tag="JN701_003253"
     CDS             complement(2749..3906)
                     /gene="mdoC"
                     /locus_tag="JN701_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY7365602.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4300..5835
                     /gene="mdoG"
                     /locus_tag="JN701_003254"
     CDS             4300..5835
                     /gene="mdoG"
                     /locus_tag="JN701_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY7365603.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5828..8371
                     /gene="mdoH"
                     /locus_tag="JN701_003255"
     CDS             5828..8371
                     /gene="mdoH"
                     /locus_tag="JN701_003255"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY7365604.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2151 a         2018 c         2219 g         2126 t
ORIGIN      
        1 ccattattcc tgaagtcact cttactcaat cttgtctgtg cagagtacaa atattgtccc
       61 tgcgcgaagg cggttcaggg caaagtcaga cgaagcaaga aaagaccctc tcattgcctg
      121 ctaatcaacc tattgctttg acgaagttga gtttaaatat ttccccggaa gatcgggtga
      181 aaatagttgt tactgtttct gatggacagg cacttcattt atcacaacaa tggccgccct
      241 cttcagaaaa gtcttaattt gttgaaatgt cgagcataag atgaatctgg agagaatggt
      301 ctgctgcgaa tcagccaacc tgaaagtatg gataacacaa ccctcaagga tgactaatca
      361 ttgaggaaat agaataaatg ttcagacctt ttttaaactc tcttatgctc ggcagtttgt
      421 tttttccttt tattgccatc gcaggaagca ccgcgcaagg gggcgtgatc catttttatg
      481 gccaaattgt ggaaccggca tgtgacgtca gcacccagtc atcacccgta gaaatgaact
      541 gcccacagaa tggttctgtt ccgggaaaaa cctattccag caaagcgtta atgagcggca
      601 atgtcaaaaa cgcgcaagta gcctcagtaa aagtgcagta tattgataaa cagaaaaagc
      661 tggctgtaat gaacatcgaa tataactaag tttctgaaca acgcacgttg ctgagtagaa
      721 aaatgcgatt taaccaaaaa gcctgctgta cacttaagaa acaagaaggt gttaaggagg
      781 cgttatgaaa acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt
      841 gattgtgaat gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca
      901 tcgcgcagcg ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga
      961 ttgccctacg gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt
     1021 acacaccgtc gggccagtct ggcgtggtgg tgaacaaaac gaagaccagc ttttgcagga
     1081 tgcctatctc aatagtctgc gactggtggc ggcaaacagc tatacgtcag tggcatttcc
     1141 tgcaatcagt actggggttt atggttaccc tcgtgcggca gcggctgaaa tcgcagtaaa
     1201 aaccgtttca gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta
     1261 tgatgaagaa aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt
     1321 tgccccggct ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc
     1381 ttttccctct ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg
     1441 cagagcatac gctggatgtt cagtattaca tctggcagga cgatatgtcg ggtcggttac
     1501 tgttttccgc cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg
     1561 acaacaatac gcccggactt gacgatattt tacgcttgct tgacagccat ccccgcattg
     1621 aagtccggct ttttaatcct ttctcgtttc gcttgctgcg tccgcttggt tatatcaccg
     1681 acttttcccg tctcaatcgc cgtatgcaca ataaaagttt cactgtcgat ggcgtggtta
     1741 ccttggtggg aggacgaaat attggtgatg cctattttgg ggcaggggaa gagccacttt
     1801 tttcggattt agatgttatg gcaataggac ccgtggtaga ggacgttgcc gatgatttcg
     1861 cccgctactg gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag
     1921 gtgaaatggc ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc
     1981 gttacttacg ccaaatggaa tccagtccgt ttataaatca tctggttgat ggaacgttgc
     2041 cgcttatctg ggcgaaaaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg
     2101 caaaacggca ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac
     2161 gcatcgatat tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac
     2221 ggatggtgag aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg
     2281 ttgctgtcgt ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg
     2341 aattatatga actcaagccg acgcgtgaac aaagtagtac gttacacgat cgcggcataa
     2401 ccggtaattc cggtgccagc ctgcatgcta aaacctttag catcgatggt aaaacggtgt
     2461 ttatcggctc tttcaatttc gatccacgtt cgacattgct caatactgaa atgggcttcg
     2521 tgatagagag cgaaacgctg gcacagttaa ttgataaacg ctttatacag agccagtatg
     2581 atgcggcctg gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg
     2641 caaagaaaga gattattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg
     2701 tcagactggc gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg
     2761 tgctttatcg ttctcacgct taacaaccgg tttaccagaa aacaaaaact tcagtaacgg
     2821 gatgcgtaaa tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca
     2881 gagaaaacca agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt
     2941 taacgggtgg tgaaccagat agataaatag cgatgcgtta acaaagtaag tcacccgcgc
     3001 tgactggaag ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacagacc
     3061 gaggaccatg gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg
     3121 ttggttgagt aaataagcga caaatgccaa tgctgctgca agggtacagc cacgagacgg
     3181 cgtggtaaac aaggctttaa gatgagggaa aatgaaagcc agtgcaccga ggataaagaa
     3241 cggcaaataa aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg
     3301 cggatacaca ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa
     3361 aatcaccgat agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt
     3421 atttctgatg cgtttaaata tccatacgca cagcgtcgtc atgactacta acaccagtaa
     3481 aaaccacagg tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgacag
     3541 cccaggccaa ctttccgctt tccctttgac gtattgcagc ataataaatt gcggcaatgt
     3601 cagtaagggg atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca
     3661 ttttttcaag ggatagcgta aaaaaagcat gtaggaaaag tagccggata taacgaaaaa
     3721 taccagcatg cggaacgagt ggatgaagtc attaaaaagg gtcagccaca atgacggttc
     3781 ggcgctattc acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc
     3841 taacaacatc agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg
     3901 attcatatat ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt
     3961 aggcttgttg ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta
     4021 tgcatagcat aatcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat
     4081 aaaattgctg gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa
     4141 gtgaaaacgg ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga
     4201 ttctgttgtc ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa
     4261 agggggaagt gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg
     4321 agtgctgcag taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat
     4381 gtcgcaaagc aagctcaatc cttagccggg aaaggctatg aggcgcccaa aagcaacttg
     4441 ccctccgttt tccgcgatat gaaatacgcg gactatcagc agatccagtt taatcatgac
     4501 aaagcgtact ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg
     4561 tacttcgata ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa
     4621 tacagcccgg attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac
     4681 cttggttttg ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa
     4741 atcgtcagca tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc
     4801 ctttctgccc gcggcctggc aattgatacc gccttgccat cgggtgaaga atttccacgc
     4861 ttcaaagagt tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca
     4921 ttgcttgact cgccgcgcgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac
     4981 acggttgtgg atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actggggggt
     5041 gcaccgttaa ccagtatgtt cctgtttggg ccgaaccaac cgtcgcctgc aaataactat
     5101 cgtccggagt tgcacgactc taacggtctc tctatccatg ccggtaatgg cgaatggatc
     5161 tggcgtccgt tgaataaccc gaaacattta gcggttagca gcttctcgat ggagaacccg
     5221 caaggcttcg gtctgttgca gcgcggtcgt gatttctccc gctttgaaga tctcgatgat
     5281 cgttacgatc ttcgtccaag cgcatgggtg actccgaaag gggagtgggg taaaggcagc
     5341 gttgagctgg tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg
     5401 acgccggatc agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc
     5461 agccgtgatg aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt
     5521 tcaacggggg atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt
     5581 gtggtcgatt ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg
     5641 caaaccagca ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt
     5701 accaaaggct ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aaccactgaa
     5761 atgcgtgctg cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta
     5821 cctgccaatg aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa
     5881 agcggcattg ccgaagactg atatccgcgc cgtacatcag gcgctggatg ccgaacaccg
     5941 cacctgggcg cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc
     6001 ctggccagat tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct
     6061 gaaggcgatg ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc
     6121 ggtaggccgt ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg
     6181 tttgaccaaa gaagagcagg agagcgagca aaagtggcgt accgtcggta ccatccgccg
     6241 ttacattctg ttgatcctga cgctcgcgca aactgtcgtc gcgacctggt atatgaagac
     6301 cattcttcct tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt
     6361 gtgggtttcc tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctctt
     6421 tgcggtactg ttctgttggg tgtccgccgg attctggacg gcgttaatgg gcttcctgca
     6481 actgcttatt ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt
     6541 aaacccggag catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt
     6601 ttttgctggc ctgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt
     6661 tgatgtctac attcttagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc
     6721 ctggatggag cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg
     6781 tcgccgcgtg aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca
     6841 gtacagctac atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttgtgcgg
     6901 gctggtgcgc ctgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc
     6961 gtccggtatg gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatggacc
     7021 actgtttaca gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa
     7081 cgcgattatc cgcgtgaaac cgtttatcga gcactgcgca ctggctccgc tgccgggcga
     7141 aggttccttt gccggttcaa tcctgtcaca tgactttgta gaagcggcgt tgatgcgccg
     7201 tgcaggttgg ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattaccgcc
     7261 taacttgctt gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt
     7321 ccgtctgttc ctggtgaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt
     7381 gatgtcttat ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca
     7441 ggtagtacat gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt
     7501 gtggccgcag tggcgtcctg agctggcgat tgcacttttt gcttcgacta tggtgctgtt
     7561 gttcctgccg aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg
     7621 cggcttctgg cgcgttacat tatcgttgct actggaagtg cttttttccg tgctgctggc
     7681 tccggtacgc atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt
     7741 ggtgtggaat tcaccgcagc gtgatgatga ctccacttcc tggggtgaag cgttcaaacg
     7801 ccacggctca cagctgctgt tagggttagt gtgggctgtt gggatggcgt ggctggatct
     7861 gcgtttcctg ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc
     7921 ggtgatttcc agccgtgcca ccgttggtct gcgaaccaaa cactggaaac tgttcctgat
     7981 cccggaagag tattcaccgc cgcaggtgct ggttgatacc gatcgcttcc ttgagatgaa
     8041 tcgtcaacgc tcccttgatg atggttttat gcacgcggtg tttaacccgt catttaacgc
     8101 tctggcaacc gcaatggcga ccgcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg
     8161 tgaccgccac gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg
     8221 cctggtgctg ctaagcgatc cggtgacgat ggcccgtctg catttccgtg tctggaattc
     8281 cccggagaga tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc
     8341 attgcgtaaa ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta
     8401 tcaggcctac atcgttcctg caatttattg attttgcaag attttgtagg ttgggtaagg
     8461 cgttcacgcc gcatccggca aaaacaacga gccaataaaa ataccggcgt tatg
//
LOCUS       DADLTD010000174         8312 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLTD010000174 DADLTD010000000
VERSION     DADLTD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.175"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(95..1765)
                     /locus_tag="JN701_003256"
     CDS             complement(95..1765)
                     /locus_tag="JN701_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY7365605.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGVSQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTWLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRTSDYLAIEIP"
     gene            complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="JN701_003257"
     CDS             complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="JN701_003257"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY7365606.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="JN701_003258"
     CDS             complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="JN701_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY7365607.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="JN701_003259"
     CDS             complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="JN701_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY7365608.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="JN701_003260"
     CDS             complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="JN701_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY7365609.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="JN701_003261"
     CDS             complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="JN701_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY7365610.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6574..>8312
                     /locus_tag="JN701_003262"
     CDS             6574..>8312
                     /locus_tag="JN701_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAY7365611.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLR"
BASE COUNT         2076 a         2052 c         2075 g         2109 t
ORIGIN      
        1 atgctctcgg caaaatcgcg atgttgttta gttgacgatg tgttttttgc gccgatgccg
       61 gatgcagcgt ttacgccgca tccggcagcc gtgtttatgg gatctcaatg gctaaataat
      121 cgctcgtccg ccgccgaact tcatccacgc gggccatgtc gtttgactgt agcgccgcat
      181 caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc acccagcgct cgcgcccaca
      241 actcttccag tttggcttta aaggtgcgat tgatgagcat atcgcgcgga taaattttca
      301 atgcggataa ccacgtccga ctctcttcaa tctgctgcgc gctcaatgtt accgggctgt
      361 ggttaatcac tctggactta acgctgccgt cttccagaag cacgtcaact tcaagcaacc
      421 cgttgatatc ataactaaag cgaatatcaa tcgactgata agccccggtt ttcttcaacg
      481 gcacatcgaa ggattcaacc agaatgttat ttttaacttt gtggttttct ccctgataga
      541 cgttaaccgt aattgaatcc tgctccgggt gcatggtcga gtaagtttct acacgcgaca
      601 ccggtactgt ggtgtttcgt tcaataatcg gcgagaaaat gccggaaacg ccctggcggt
      661 taacttcaac gcccaacgag taagggcaaa tatcagtgag gattacctct tcaatatctt
      721 cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag cgcgacaatg gtgctcggat
      781 cgtaactttg atacggtaat ttgccaaaca gacgcacggc gattcgctgc accagcggca
      841 tttgtgacac gccgccaacc agcaccagac tgtcgatttg actcggcttc agacgcgcat
      901 cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag cagcggcaac cacaaatctt
      961 ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcttc ctgatactgc cagcgaatgt
     1021 gcaatggcga ttgattgcta cattttgccg cttccacaca ggcgtacaag gctgccagtt
     1081 cactctcgtt aagcgtggtc ctggcaactt ccgcgcgttt taaaacctca tcgactagca
     1141 tatgggtaaa atcttcgcca ccgaggaagc tatcgccagc ggaggcgtga atttcaatca
     1201 ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc gccaccgaga tcaaaaacca
     1261 gcgagcgggt attttgttgc gtatgcaggc catatgccat cgcagctgct gtgggttcat
     1321 taattaagcg taccgcattt aacccggcta actccgcagc taaacgggta tgcttgcgtt
     1381 gttcatcgct gaaataagcc ggaacggaga tcaccacatc tttaatcgga cgttgcagaa
     1441 attcttcggc atcttctttt aatgagcgta ataccaaaga ggacagttct ggcgcgttaa
     1501 atgtgtcgct gcctaaccgc cagttggtat tactgcccat tgcgcgttta aataacgctg
     1561 ccgttttatc cggatgcgaa gtacgccgtg atacagccgg ttttccgact aaaatatgat
     1621 tattttcatc catgctaatt atggatggtg ttaaatattc accgaactta tttggaatta
     1681 attgcgcggc accgtctttc cagacggcaa ttaaactatt ggtagtgccg agatcgatac
     1741 caatggtgag ttctgcatta tccattgcat ttatatcttg aaagaaaaag agataaacag
     1801 attaagacca cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa
     1861 tttcagacga tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacagt
     1921 ggcattcggt ttattgtcag tcagataata ataatcaaca accgtcatac cctgggtata
     1981 tttcccctgt gtttccacgc caacccagcg ctcaacagtg gtaaacaact cgggtttcaa
     2041 cagccaggcg atggtgcatg ggtcatgcag cggtgcaccg acaaagcccc atttttcgtc
     2101 tttatgatat tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc
     2161 aatcgcgcgg aaacgctcgg tgtcttcaat gtggatttgc gctttatgag taacatccag
     2221 accggccatc accactggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac
     2281 gtaaatatta aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat
     2341 cacgatacgg gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt
     2401 agtttgcggt ccggtagaca caatcgtgac aggttccgca ctttcgcgca gcgttttcgc
     2461 catcagctct accgccgtac agttttgcgg tgcgaacgtc ggttccggta atgccgggcc
     2521 atccagaccg ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt
     2581 taccgcgccg cctgctaccg gaatatcggt gcgattaagc aaggtcagca tacgcagaac
     2641 attgcgtaag gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc
     2701 aagctctggt gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca
     2761 atctaacaga attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg
     2821 taacgccagg atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact
     2881 tgggtatgcg cgccagagtg attaatgcag gattttcgcg aggaagtctt tcgcgcggtc
     2941 cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcgtc
     3001 cataaagatc acccgattcg ccactttgcg ggcaaagccc atttcgtggg tcaccaccat
     3061 catggtcatt ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc
     3121 cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaacgc
     3181 gcgagcgatt gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc
     3241 atgtgcggaa agtcccacgc gctccagcaa tttcaatgct ttttcacgag cgggcgcttt
     3301 atcgcgttta agcactttca cctgcgccag agtcaggttt tcaataatcg acaaatgggg
     3361 gaacaactca aaatgctgga acaccatccc gacgcgggaa cgcagctttg ccagatcggt
     3421 tttcttgtca ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag
     3481 gccgttgacg gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac
     3541 cacttcgcct tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca
     3601 ttttgaaaca tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac
     3661 aacgacgcgc taagactaat aacgaaataa acaaatccgg caaacaggat catctcaacc
     3721 tgcgtaccat cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac
     3781 acatacacca gtgaggtatc ctggaacagt acgatgccct gagtgagcag aagcggcacc
     3841 atcgcgcgga acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc
     3901 aacgccagcg cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc
     3961 tctgaatagt aggccgcttc aaacatcgaa aacgccacca tcgccgaaat taaacggata
     4021 tcattttttg gtgataatcc cagcacgttt tgcagaaaac ctggcacgat caggtaaaac
     4081 cacagcaaaa ccataactaa aggaatcgag cggaatatgt taacgtaggc tttggcaaac
     4141 cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata
     4201 ccaatcacga ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc gagcagatat
     4261 ggcagggaag ggacaatgga actccagtca aactcgtaca ttatttgccc cccatgttgc
     4321 caggcaggcg aactttacgt tcaaccagcg tcattaccag catgataatc gcgttaatca
     4381 acacatacgc cagcgtaata gcggtaaacg actcccaggc gtgggctgag taatcaagca
     4441 atttacccgc ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt
     4501 tcaccaggtt catcatctcg gaagtcatcg gcgggacgat aacgcgataa gcattaggca
     4561 gcagtacgta tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattttttt
     4621 gcccacgcgg caacgactga atggcggcgc gtacctgttc gcaaacacgg gcggcggtaa
     4681 acagccccag gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct
     4741 taaaccacat gccgattttc tccggcaaca gctccgggat caccagatac caggtaaaga
     4801 attgcacaat caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga
     4861 gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa
     4921 tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat
     4981 aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca
     5041 taaatctact ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac
     5101 ccagcgtatt gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg
     5161 tgctacgtaa caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt
     5221 gctttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg
     5281 ttcaggtttt tcggcggaat tggatttttg aaccatttat caaaccattt ttccgcttca
     5341 ccggaagtct gcacctgagc gatagtgtca tccatcagct ttttgaactg cggatcatct
     5401 ttacgcaaca tgcaaccgta ggcttcctga gactgcggct tgccgacgat atcccagttg
     5461 tctggtttct tcgctttcgc acgttcaccg gccagcaggg catcatccat cataaaggca
     5521 acggcacgac cgctttccag ggtgcggaaa gagtcaccgt gatctttggc gctgatgatg
     5581 cgcatattca ttttttgctc ttcattcagt ttgttgagca aaacttcaga ggtagtgccg
     5641 gaagtgacga ctacggcttt gtctttcagg tcggcaaaat ctttgatatc gccgcccttt
     5701 ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag agaaagccgc ctgtttttgg
     5761 cgttcgacgt tgttggtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt
     5821 ggaatacggt tttgtgaggt aatcggaatc agttttacct gcaagtccgg tttgttgagt
     5881 ttctttttca ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt
     5941 tgctgattgt cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcaca
     6001 ccgtttttgg cgattttgtc cagcgtactg cctgctgccg gggcggcgtc atctgcctgt
     6061 gccagtcctg cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata
     6121 tccaactcct ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt
     6181 gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct
     6241 aaatgtaaag atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga
     6301 agcaaaattc ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg
     6361 gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa
     6421 atacttaggg gcaaattaat tacgattgta attttaggct gaaaaagtca tatttcggac
     6481 agaattatct tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc
     6541 tttttacctg tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta
     6601 aatgttcaat tacccgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt
     6661 tatctcgcac aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggc
     6721 gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt
     6781 ctgcacagta gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc
     6841 gtggcggaac agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc
     6901 tttggtgggc tcatcagcgc ctgttatgcg ttacgcgata gtgatcagat agtcatcagc
     6961 gttggggcat ccggggcaat tatgggaata gctggcgctg cgatagcaac acagcttgct
     7021 tcaggtgcgg gcacatacca taaaaaccag cggcgagtat tttctctgtt gggtatggtg
     7081 gcgctgacac tgttgtatgg tgcccggcaa acaggaatag ataatgcttg tcacattggc
     7141 ggcctgattg cgggtggcgc gttgggttgg ctgagcgcgc gtttatctgg gcaaaaccga
     7201 ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg
     7261 cttgcgcagc agcagatgga tgagtcagtt ttacaagtca ggcaaagcct gcgtgaagcg
     7321 ttttatccac aggagattga acaagagcga cggcaaaaaa agcagcagtt agcggaggaa
     7381 cgcaacgccc tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat
     7441 ttgctcgctg agattgccga tatccatgat atggcgatca gtcgggatgg taatatgttg
     7501 tatgccgcaa ttgaaaacac gaacagcatt gttgttttcg acctcggaca aaagaaaatc
     7561 ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt
     7621 aaagatcagg gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgact
     7681 tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtcg
     7741 attaccaccg gtgcacatcc tgacagcctt atcctctcgc gtgatggcac aaaagcctgg
     7801 gtgatgaatc gcaccagtaa tagtgtgtca gcgattgatc tggtgactta tcagcatgtg
     7861 gcggatatcc cgctggagaa atacgacggg gcggggacga gcggtaaacc aggcgcctgg
     7921 gttatggcac tttccccgga tgaaagaaca ttactggttc cgggagcagg cagaggtaac
     7981 atcgtgcgga tcaataccat cacgcatcag aaagaagact ttcccgcagg tgatgcgcgt
     8041 ggaacgatat cggcgatgcg ttttcgacct gaaaacggcg aggttatttt tgcagatagt
     8101 caggggattt cacgtataag cgtaggggat caacaagcca gcattatgac gcaatggtgt
     8161 agcaggagcg tttattccgt tgagggtatt agcccggacg gtcaatattt agcgttggtg
     8221 tcatatggcc tgcaaggtta tgtcatcctg ctcaatatta atgccgggca gattattggc
     8281 gtttatcctg ccagctacgt taatcacctt cg
//
LOCUS       DADLTD010000175         8240 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLTD010000175 DADLTD010000000
VERSION     DADLTD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.176"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN701_003263"
     CDS             complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN701_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY7365612.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(1803..4067)
                     /locus_tag="JN701_003264"
     CDS             complement(1803..4067)
                     /locus_tag="JN701_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HAY7365613.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     IGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVTILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VYDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(4274..4558)
                     /gene="ihfB"
                     /locus_tag="JN701_003265"
     CDS             complement(4274..4558)
                     /gene="ihfB"
                     /locus_tag="JN701_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY7365614.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(4718..6391)
                     /gene="rpsA"
                     /locus_tag="JN701_003266"
     CDS             complement(4718..6391)
                     /gene="rpsA"
                     /locus_tag="JN701_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY7365615.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(6502..7185)
                     /gene="cmk"
                     /locus_tag="JN701_003267"
     CDS             complement(6502..7185)
                     /gene="cmk"
                     /locus_tag="JN701_003267"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY7365616.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(7358..8121)
                     /locus_tag="JN701_003268"
                     /pseudo
     CDS             complement(7358..8121)
                     /locus_tag="JN701_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
BASE COUNT         2106 a         2174 c         1931 g         2029 t
ORIGIN      
        1 agaccagatt ttttcgatca ttggccaaac tgcattttgt gaagttgcgc gtaaacgccg
       61 cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc ctcgacgacc
      121 acgatttcgt ctgccttttc aatggtagac aagcggtggg caatcaccag agaggtacgg
      181 tttttctgca actcatccag tgccgcctga atcgcacgtt cggattcggt atccagagcc
      241 gaggtagctt catccagaat cagaatcggg ctatcacgca gcaaggctcg agcgatagca
      301 atacgctgac gctgaccgcc agagagcagc acgccgtttt caccaatcac tgtatcgaga
      361 ccgttatcca tcttattgat gaagtccatg gcgtaggcca tacgcgccgc ttcttcaatt
      421 tgctcacggc tgtactgttc agtccgtgcg taagcaatgt tgttagcaac cgtatcgtta
      481 aacagatgga cattctgcga caccagagca acctggttac gtaacgacgc cagggtatac
      541 tcgcgcagat cgtgaccatc catcaggatt tcgccttcat caatatcgta aaaacgagtg
      601 atcaggctgg cgatggttga tttacctgaa ccagagcgtc caaccagagc aaccgtcttc
      661 cctgccggaa ttttcaggtt gatgttacgc aatgcaggta cgtcacgtcc cggataagta
      721 aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg
      781 tctttctcct gctcactgtc cagaatggta aacagcgtct gacaagccgc cataccgcgc
      841 tggaactggg cgttaacgtt ggtcagtgat ttcagcggac gcatcagtgc aatcattgaa
      901 gagaaaacaa cggtaatcgt accagcagtc aggctatcca tgacacttgg gaagctcgcc
      961 gcatacagaa caaacgccag cgccaaagag gcgatcagct gaatgatcgg atcagagatg
     1021 gaagaggctg aaaccatttt catcccctga agacgcattc ggttgctgac tttatcaaag
     1081 cgtttcgttt ccacttcctg accaccgaaa atcaatactt ctttgtggcc cttcagcatt
     1141 tgttcggcgc tggtggtcac ctgccccatg gtgttctgca tgtttttact gatgttgcga
     1201 aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg
     1261 atcgacagtt gccaactgta atagaacatc atgatgaaca ggccgatgat tgacgcacct
     1321 tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag caacctgttc ggaatcgtag
     1381 gtaatacgtg acaacagcgt ccccgttgac tgtttgtcaa aaaatgaaac cggcattccc
     1441 atcatgtgac caaacaggcg gcgacgcatg gtcattacca cctttcctga tacccaggag
     1501 atacagtagc tggagacata gctggtgata ccacgtaaaa tcatcagccc gatcaccacc
     1561 agcggcatcc acaccagcac ggagcgatct gttttaccaa agccatcatc aagaagtggc
     1621 ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc
     1681 acgatcagac ccgctttaaa aggcgcaatg gttggccaca gtcggcggaa cgtctgccac
     1741 gtagagagat ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa tagccgcata
     1801 ttctacccgt tatccactgg cacgccaaac cactgatgat accaacgagg taaaatttgc
     1861 tctctgaggc tgctaatccg ccagccttgc gctgaaaaat tgaccgtcac ttgaccctga
     1921 tgtggagtat caagccattg atatccttgc tgttgatagc gatgcttaac tttattagag
     1981 ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
     2041 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
     2101 gtctgcactt gctgccagta acggcttagc atcttttgtt cagcggggac ttcaatatca
     2161 ccggttagaa gaatgctatt cgtcccgtca taaaccttaa ccacacagga atggttattt
     2221 cctttatcgt tgctagcttg taaaggccag tgcacgctga aacgcaatcc ttgccattgc
     2281 cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact tctgatccat
     2341 aacatcggcc atgtgtgcaa tattgagtcc agccctcccc ggtgatccag atgttcatga
     2401 ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt
     2461 tgcccactat ccccttcggg ccaggccagt cctgtgtcat agagaatcgc tttgccgttt
     2521 ctggctatca ccattgccag cccttgcccg acatcaagca tgtacacctg ccactcgtca
     2581 ggtcgaggtt tttgccacag cggccagcac atcagcaagc ctacagccac acacattgct
     2641 ggcaacgttc gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat
     2701 agccattgcc aacgttcagc aatgttgatc cacccttctg gcaaactctt taacccccag
     2761 aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc cttgctccag gattaacggc
     2821 ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag
     2881 ggaattgcta atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt
     2941 tgcacaggca taagcaggag tgtgattccc agttgcaaat gaatgaggga aacaactgca
     3001 cgcaataccg gcggcagttg ccactcagga cagggaaacc actggtacca aaaaatcagt
     3061 gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggtaac aggatccatc
     3121 agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt
     3181 aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt
     3241 cccgtcaacc aggcataaaa agcagcacag cagattccac caattaatgg catttgccag
     3301 tggatccagc gcccaggcag aaaaacttgc ccaccgcgaa tgagtccggc agccagcaac
     3361 gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcacgc
     3421 atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata
     3481 accgcattcc acatataggg ttgcagcgtt gtttgtagtg acgccagata ttgtgcacgc
     3541 aggctacaat tcggttcaat gacacttgcc tgcagaaagc ggccgatgag cggctgatgc
     3601 tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca
     3661 cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg
     3721 ccatacagca caagacctgg cgcagggaat atacgtcgac cttgtagatg agtaatttga
     3781 ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg
     3841 ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa
     3901 aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag
     3961 gcgaagagag tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac
     4021 ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg
     4081 tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga
     4141 ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct
     4201 tccagttcct gattagagaa atgaaaaaag cacccgacag gtgcttttct cgcgttcaag
     4261 tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa
     4321 agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg
     4381 tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc
     4441 cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct
     4501 tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga
     4561 ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg
     4621 ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca
     4681 acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga
     4741 aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga
     4801 ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt
     4861 tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag
     4921 cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag
     4981 ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc
     5041 ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca
     5101 aggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt
     5161 ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa
     5221 ccaggccgtc gatgccgccg tctaggccga tgaagatacc gaagtcagtg atagacttga
     5281 ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag
     5341 ctttgcactg tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca
     5401 taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc
     5461 aatccatttc ggaaacgtgt accaggcctt caacgccttc ttcgatttca acgaagcagc
     5521 cgtagtcggt caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag
     5581 cgatagctac ccacggatct tcgcccaact gtttcaggcc cagggataca cgggtacgtt
     5641 cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg
     5701 gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca
     5761 gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt
     5821 cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac
     5881 gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct
     5941 ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca
     6001 ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa
     6061 caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac
     6121 gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag
     6181 caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct
     6241 cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga
     6301 tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact
     6361 cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc
     6421 cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg
     6481 gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc
     6541 tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc
     6601 cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc
     6661 caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg
     6721 cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac
     6781 cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg
     6841 acgcaataat gcttcacgaa cgcgtgggaa tgtcgcgact tgtgaagccg cattcgccac
     6901 ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc
     6961 attggttgac acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttctga
     7021 cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga
     7081 atccagcaga tgccattgca atgcttccgc catagcctta cacaaggtgc ctttccctgc
     7141 accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa
     7201 acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac
     7261 gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc
     7321 gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat
     7381 cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc
     7441 gaccgccatc cagactagcc agtttttcaa agctgccaac cagcccctag gtgctgatgc
     7501 cacgtttctt caacagatca aaggagaaat catctgcttc ggactcttta tcacgggagt
     7561 atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag
     7621 ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaaactttca
     7681 tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa
     7741 tttcgttgtc attcatcatg tccatcaggc cactgtagac acggacacag ccgttcgcca
     7801 tcgcccatgc gttgacgtcg ctggtcatat aaacctatag ttgaccggcg tgccattaat
     7861 attgttaccc aatgctttgg cgatttttgc cagacgttta ccgtacttgc tggatttact
     7921 tgcgacttga ttgccgctgt ccatttcggc acagccctga ttggcaatcg cttttgcatc
     7981 ggcatcgctt aatgttgctg ctttataggc atttaaaccg gagctgatag ccatattggt
     8041 atcaataccg tgggtatttt gacaccctgt cagtaatgct gcagaggtcg caatcgccag
     8101 cagtaattta gtattcttca tgtgaatcat ccgttgatta atcatatata aacattatgt
     8161 atcaataact tacattgaca agcgacgcta ccattaatca aaatgactca caaggtccga
     8221 aaaaaaacgc cgacaatgtg
//
LOCUS       DADLTD010000176         8146 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLTD010000176 DADLTD010000000
VERSION     DADLTD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.177"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            66..260
                     /locus_tag="JN701_003269"
     CDS             66..260
                     /locus_tag="JN701_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose carrier protein"
                     /protein_id="HAY7365617.1"
                     /translation="MQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRK
                     VPPEPVHADIPVGAVSDVKA"
     gene            complement(399..1028)
                     /locus_tag="JN701_003270"
     CDS             complement(399..1028)
                     /locus_tag="JN701_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY7365618.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1151..2797)
                     /locus_tag="JN701_003271"
     CDS             complement(1151..2797)
                     /locus_tag="JN701_003271"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY7365619.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2875..4215)
                     /locus_tag="JN701_003272"
     CDS             complement(2875..4215)
                     /locus_tag="JN701_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7365620.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="JN701_003273"
     CDS             complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="JN701_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY7365621.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="JN701_003274"
     CDS             complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="JN701_003274"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY7365622.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7314..7544
                     /gene="yjdI"
                     /locus_tag="JN701_003275"
     CDS             7314..7544
                     /gene="yjdI"
                     /locus_tag="JN701_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7365623.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7556..7828
                     /locus_tag="JN701_003276"
     CDS             7556..7828
                     /locus_tag="JN701_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7365624.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8055..>8146
                     /gene="ghoS"
                     /locus_tag="JN701_003277"
     CDS             8055..>8146
                     /gene="ghoS"
                     /locus_tag="JN701_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7365625.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSL"
BASE COUNT         2022 a         1968 c         2084 g         2072 t
ORIGIN      
        1 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
       61 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
      121 tctacttccg tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc
      181 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
      241 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
      301 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
      361 aattcacgct gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
      421 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
      481 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
      541 tccgctccct gacccattgc cagcgccgcc caccactcga ttaattcata cattgcgctt
      601 atcgccagcg ccacgcagca gactaggaac gccaccattt tacgtccgcg cacgtacatc
      661 ccgcgaacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaagtgcccc
      721 agcttgtcat acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg
      781 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaag gaaaatgagc
      841 gtatagagca gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca
      901 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
      961 taaatcagga ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
     1021 cgggtcatgg tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa
     1081 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     1141 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt
     1201 cgttaccttt gtcatcgacc aagataaacg ccgggaaatc ttctacttcg attttccaga
     1261 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     1321 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     1381 tgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     1441 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
     1501 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     1561 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     1621 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
     1681 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
     1741 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
     1801 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     1861 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     1921 atttaccgcc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     1981 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
     2041 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     2101 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga
     2161 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
     2221 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
     2281 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     2341 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt
     2401 tcgacagcgc ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga
     2461 tgatcgcgat gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggaat
     2521 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     2581 tagccgcaac ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt
     2641 gctgcgccag cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
     2701 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     2761 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     2821 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     2881 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     2941 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     3001 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     3061 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccaacg cgaccggaac
     3121 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
     3181 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     3241 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     3301 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     3361 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
     3421 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat
     3481 tgccccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag
     3541 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
     3601 ctcttcgtct ttatccagat ctttaccacg gaaccagctg aagataccga tcgccaggat
     3661 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
     3721 atcaaaggtg acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact
     3781 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
     3841 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
     3901 accgcaaaga atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg
     3961 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
     4021 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt
     4081 acctggctga aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc
     4141 cagcgcgata ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg
     4201 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
     4261 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
     4321 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa
     4381 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
     4441 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
     4501 tattttttgt tctcagggca gatatcatta ggtttaaaga ttaatcttat ctacccattt
     4561 gtgggtgaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
     4621 tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata
     4681 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
     4741 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
     4801 tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
     4861 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg
     4921 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
     4981 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
     5041 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
     5101 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
     5161 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
     5221 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
     5281 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
     5341 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
     5401 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
     5461 ctccgcgacc attgcgtcgt catcgataat taatacattg ctcatctgtt tgacctctcc
     5521 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
     5581 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
     5641 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     5701 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     5761 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     5821 gtggtaatca gcgttgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg
     5881 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     5941 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctt atagttattg
     6001 gctgttttga gactgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     6061 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
     6121 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
     6181 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
     6241 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
     6301 tctaccacct gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta
     6361 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
     6421 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg
     6481 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
     6541 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
     6601 tcattgattt gttgggtcac gcggcttaac tcaaggccgc tcaccaccac gccaatttgt
     6661 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
     6721 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
     6781 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc cgtaacgaca
     6841 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
     6901 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact
     6961 gccagtgcct tgttggccag cccgtctcgt gtcatatcac tgatttgcga gaagtaaatc
     7021 agatgcacca ccaatagcac cgagaactgt accgcgctga ccattaagat cactgtggta
     7081 ctcaatttca tcggacgttt gagtaacatg cggtagggca atgaatgtct catcagcttc
     7141 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa
     7201 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
     7261 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
     7321 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
     7381 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
     7441 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga
     7501 ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
     7561 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
     7621 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
     7681 aagcctgaaa gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
     7741 tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac
     7801 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
     7861 ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
     7921 agagagcgcg tttttttgtc ccacgtcatc ccctttactg agcaaaaaaa agaatatctc
     7981 ctatatgaga atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg
     8041 ataggagtca tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt
     8101 atccatcatc ttattcctca gtgttcttaa ggttaagatc attgat
//
LOCUS       DADLTD010000177         8099 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLTD010000177 DADLTD010000000
VERSION     DADLTD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.178"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(57..133)
                     /locus_tag="JN701_003278"
     tRNA            complement(57..133)
                     /locus_tag="JN701_003278"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Asp,seq:gtc)
     gene            complement(266..997)
                     /gene="dnaQ"
                     /locus_tag="JN701_003279"
     CDS             complement(266..997)
                     /gene="dnaQ"
                     /locus_tag="JN701_003279"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY7365626.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1062..1529
                     /gene="rnhA"
                     /locus_tag="JN701_003280"
     CDS             1062..1529
                     /gene="rnhA"
                     /locus_tag="JN701_003280"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY7365627.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1526..2248)
                     /locus_tag="JN701_003281"
     CDS             complement(1526..2248)
                     /locus_tag="JN701_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7365628.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2282..3037
                     /gene="gloB"
                     /locus_tag="JN701_003282"
     CDS             2282..3037
                     /gene="gloB"
                     /locus_tag="JN701_003282"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY7365629.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3109..4467
                     /gene="mltD"
                     /locus_tag="JN701_003283"
     CDS             3109..4467
                     /gene="mltD"
                     /locus_tag="JN701_003283"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY7365630.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4516..5139)
                     /locus_tag="JN701_003284"
     CDS             complement(4516..5139)
                     /locus_tag="JN701_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY7365631.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5143..5943)
                     /locus_tag="JN701_003285"
     CDS             complement(5143..5943)
                     /locus_tag="JN701_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7365632.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6184..7098
                     /locus_tag="JN701_003286"
     CDS             6184..7098
                     /locus_tag="JN701_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7365633.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7095..7862)
                     /gene="dkgB"
                     /locus_tag="JN701_003287"
     CDS             complement(7095..7862)
                     /gene="dkgB"
                     /locus_tag="JN701_003287"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY7365634.1"
                     /translation="MKDYVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPR
                     HELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPNDEVSVEGVMQAL
                     LEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHG
                     IHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRKNLES
                     NLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2154 a         1954 c         1975 g         2016 t
ORIGIN      
        1 aaaaccgcaa aattaaaaat cttgcggttc tctgaactca ttttcatgag tgaatttggc
       61 ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact
      121 gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg aatattacgg attgcctcac
      181 ccttcgtcaa cggtttttct catcttttga atcgtttgct gcaaaaatcg cccaagccgc
      241 tatttttagc gccttttaca ggtatttatg cccgccagag gcagcttccg cccttcttct
      301 ccaccagatc aagacgggct tcatgagctg caagctcttc atctgtcgca aaaacaacgc
      361 gtaacttact tgcctgacgt acaatgcgct gaattgttgc ttcaccttgt tgctgctgtg
      421 tctctccttc catcgcaaaa gccatcgacg tttgaccacc ggtcatcgcc agataaactt
      481 ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt tcgtttactg ttatctattt
      541 cgtagcgggc acataacgca tcgaggctgt tgcgcttacc aggaaacatt ttcctcgcca
      601 ccgcaaggct atcggtgacc ttacagaagg tattggtctt cggaatatcg cgcttaagca
      661 acgaaaactc gtagtccata aagccgatat cgaacgctgc gttatggatc accaactccg
      721 cgccgcggat atagtccatg aactcatcgg ctacttcggc aaacgtgggc ttatcgagca
      781 aaaattcatc ggcaatacca tgtacgccaa aggcttccgg atccaccagc cgatcgggtt
      841 tgagataaac atggaagtta ttgcccgtca ggcgacggtt caccacttca acggcaccaa
      901 tctcaatgat cttgtggcct tcatagtgcg caccaatctg gttcataccg gtggtttcgg
      961 tatcgagaac gatctggcgt gtaattgcag tgctcatagc ggtcatttat gtcagactta
     1021 tcgttttaca gttcgattca attacaggaa gtctaccaga gatgcttaaa caggtagaaa
     1081 ttttcaccga tggttcgtgt ctgggcaatc caggacctgg gggttacggc gctattttac
     1141 gctatcgcgg acgcgagaaa acctttagcg caggctacac ccgcaccacc aacaaccgta
     1201 tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc gaagtcattt
     1261 tgagtaccga cagccagtat gtccgccagg gtatcaccca gtggatccat aactggaaaa
     1321 aacgtggctg gaaaaccgca gacaaaaaac cagtaaaaaa tgtcgatctc tggcaacgtc
     1381 ttgatgctgc cctagggcaa catcaaatca aatgggaatg ggttaaaggc catgccggac
     1441 acccggaaaa cgaacgctgt gatgaactgg ctcgtgctgc ggcaatgaat cccacactgg
     1501 aagatacggg ctaccaagtc gaagtttaag cttgtggttt acgacattgc cgggtggctc
     1561 caaccgcttg gcgaattcgt ggcttgtttt tactctgttt catcgggttt agcgttaaag
     1621 gaatagtccg tttccgggca acaataagtt gtaagcaacc aagcgcagga atatgcgcat
     1681 tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg gaaacggctg gcgtgcagca
     1741 cttcaaaatt caacaaagag agccagtcca gttgccgcat cagagtaaac atccggctgt
     1801 tatagggcga ggtttttcgc aataccggca caagtttgcg taatcccatt aaactaatgg
     1861 gattgaagcc actaatcacc agccagccat catcaatcaa tacccgatcg gtttcacgca
     1921 ataaacgatg cggatcggtg caccacggca atgtatgtgc cagtagacaa acatcaacgg
     1981 atttatcggc aaaaggaaga tgaagtggat ccgcctgcac ctggacgggc attccttgcg
     2041 cagaaacatt cacttgatga gaaaccgcgc acgcttcgca attgatttct gcgcttaaat
     2101 tgccaatctt aagcagatga aaaccataca ttttagtgaa ccacgggttg agctggcgct
     2161 ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc ccagcgatca ggagccacga
     2221 cagtttgagg gactcttgcc ggtttcatca caaccttccg tttcacacta agaggtaatc
     2281 tatgaatctt aacagtattc ccgcctttga tgacaattac atctgggttt tgaatgatga
     2341 agcaggtcgc tgcctgattg tcgatcccgg agacgcagag ccagtattaa acgccattgc
     2401 cgccaataac tggcaaccgg aggccatatt tctcacccac catcatcacg atcacgttgg
     2461 cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg gtgtatggtc cacaagagac
     2521 acaagataag ggaacaacac aggtagtcaa agatggcgaa actgccttcg ttttggggca
     2581 tgaatttagt gtaattgcca caccgggtca cactttagga catatctgtt acttcagtaa
     2641 accttatcta ttttgcggcg acacgctgtt ttctggtggg tgtggtaggt tgtttgaagg
     2701 gacagcatca caaatgtatc aatcacttaa taagttaagt gcgttacctg acgatacatt
     2761 ggtatgttgt gctcatgaat atacgttatc aaatatgaag tttgctttga gtattcttcc
     2821 gcacgatttg tccataaatg attattatcg taaagttaag gagttacggg caaaaaatca
     2881 aataacacta cccgtaattc tgaaaaatga gcggcaaatt aatgtttttt taagaacgga
     2941 agatattgat ttaattaatg taattaatga agaaacatta ttgcaacaac ctgaagagcg
     3001 ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg tcatcggctc
     3061 cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat gaaggcaaaa
     3121 gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa cgttcaacag
     3181 cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac aagtcaggca
     3241 cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc tttcattggc
     3301 gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa acagaaatat
     3361 ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta tatgtactgg
     3421 atagcagggc aagttaaaaa acgtaacatg cctatggaac tggtactact acccatagtg
     3481 gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat ctggcagatc
     3541 attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga cgcgcgtcgc
     3601 gatgttgttg cttcaacaac tgccgcgctg aacatgatgc agcgtctgaa caagatgttt
     3661 gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg ggtcatgaag
     3721 gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt accgttgccg
     3781 caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct caaaaacagc
     3841 aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc gcgtgtgcac
     3901 ctgagcagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc cgtcagcaag
     3961 ctgaagacat tcaacgctgg cgtaaaaggc tccacgctgg gcgcaagtgg cccgcagtac
     4021 gtgatggtgc caaagaagca tgcagatcaa ctgcgtgatt ctctcgcttc aggcgaaatt
     4081 gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg tgtttacacc
     4141 gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc tcggcgtaag caccaaagat
     4201 ttgcagcagt ggaacaaact gcgcggatct aagctgaagc caggccaaag tttgacgatt
     4261 ggtgcaggca gtagcgcaca gcggttggca aacaacagcg atagcattac gtatcgtgtg
     4321 cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat caaagatgtg
     4381 atgcgctgga acagtgacac tgcgaatctg caaccaggcg ataagctgac gttgtttgtg
     4441 aaaaacaaca acatgccaga ttcctgacaa accagataat aaaaaaggca ccgattcccc
     4501 cggtgccttt tttatttatg ccgctttatg cgcttctacc atgatgatat cactggtgaa
     4561 agagccatca gtctgcaagg aaaaatacgt tctcacctct gtcgatgcgc tctgttggta
     4621 aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcacgaccc atgaagaaaa
     4681 ttccagcggt aacttatctg taattaaatt atcaactata agattggctt cattgattaa
     4741 cgtcaaccac gcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta atgcttctac
     4801 cgcctgtaac cagatgtcgc gcactgggtg acccggagac attacatcca tcacaatcag
     4861 cctaccgcca ggtttcaata tcctattcac ttctcgcagt gctgcaccaa catcatgcca
     4921 gtgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa atggcagact
     4981 tgcggcatat ccctggcggg tggtgatatt tttcagttgc cgggcttcgg cagcttgtgc
     5041 cacgacatcc agcatgtggg ccgataagtc atacgccacc accgcgctca cgttttgcgc
     5101 agcgccaaac gttggtctgc cctgtggcag acctgacata ccttatttat caggcttgcc
     5161 gggactgaat tcaacgagta gcggattatg atcggaagcg cgcgtaacca gtacagaagc
     5221 ttcactgacg ttcagaccac ggtagaaaac aaaatcgagc gggcgaccaa acgcccgacg
     5281 gcgctgatca tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa agcgatataa
     5341 cgcgttcatc cttctacggc tccaggcatt gaaatctccc gccataatga ccgggccgct
     5401 gtggtgagct atctgatcac caataggaag taactgctta ctatagacat ccacgcccag
     5461 actgaagttg acggcgtgta tattaaccac catcaacagg cgggtgtcag gtaatggata
     5521 gaccgtcacc agtgccgact tcgccagacg caaaatgggt tctcgttcac gtaacgggca
     5581 gcaatacact ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt gtggcagcac
     5641 gaaagcgggt acctgatcgg cggcaagata gttagcggtc gcaaactgta ctaactctgg
     5701 cgttgtctgc gcttcctgca ataacaccag gtgtgcatct ttgccgtagt tctttaatac
     5761 cgacaaccat tcagcgcgtt gctgtttgta tatgttccac accaggatcc gaatacgctc
     5821 ttcggtactt aacggttccc caggtggtaa tgcctggccg atgctcgcaa aagaccccgg
     5881 cggtaagatc ctttccgcag gttgtccggc aacatagcgc atggcatagg tgttttttcg
     5941 cacttttgac tttgaaacct ctattacgtc aacccttccg cactcgggag ggttctacta
     6001 ttatagggat tttagctcac tctttcaacg agcaattgta tattgttatg taagcaagtg
     6061 cttacactgg tcagaatttt gctcattatg gagaacatta aatcgcatga catatcacgc
     6121 atcctgatgg tttaatgaca aataaattca aaatatctct tctattttgc aacaggagca
     6181 aaaatgaaag ccacgtcgga agaactcgcc atttttgttt cggtcgtcga aagcggcagc
     6241 tttagccggg cagcggaaca attagggcaa gcaaactcag cggtaagccg ggcggtgaaa
     6301 aagctggaga tgaaacttgg cgttagcctg cttaatcgga ccacgcgaca acttagcctg
     6361 acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg
     6421 gcagaatcag aaattatgga gacgcgtaat acaccgcgtg gactgttacg gatcgatgcc
     6481 gcaactccag tggtgctgca ctttctgatg ccgttaatta agcctttccg tgaacgctat
     6541 ccggaagtca ctttgtcgct agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa
     6601 gtggatgtcg cgatacgcgc tggtacgtta acggattcca gcttacgtgc caggccgtta
     6661 tttaacagtt atcgaaaaat tatcgcctcc cccgattata tttcccgcta cgggaagcca
     6721 gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat tcactgaacc cgcttccctc
     6781 aatacctggc cgatagcctg tagtgatgga caattacatg aggtgaaata cggtttgtca
     6841 tccaatagtg gggaaacact gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg
     6901 tccgactaca tgatcgacag agaaatcgct cgcggagaat tggtggagtt aatggcagat
     6961 aaagtgttgc cagtggaaat gccattcagt gccgtctatt acagcgaccg tgcggtaagt
     7021 acgcgcatcc gggcttttat cgatttcctt agcgagcatg taaaaacagc tcccggagga
     7081 gctgtcagag aggcttaatc ccattcagga gccagacctt ccgggctaac caggcggtcg
     7141 ttgcaatcca gtgcggcgat cgctttttta tcttcggcat caagctgtaa attttgtgcc
     7201 ttaagattac tttccaggtt tttacgttta gtagaagaag gaattactga gtaaccttcc
     7261 cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc tgcgatacga
     7321 gcaataacct catctttcag ggccttacca tacgccagcg tcatatagga agtaatatgg
     7381 atgccgtgct gtttagccca ggcaaccact ttacggtttt gcagataagg agagagttca
     7441 atctggttag tagcgatgtt ttcagcgcca acagcagcaa tcgccttttc catcaatggg
     7501 atcgtgaagt tggaaatacc gatctcacgc gtcagccctt gttttttggc ttccagcagc
     7561 gcctgcataa ccccttcaac agagacttca tcgtttggtg acggccagtg gattagcgtc
     7621 agatcaacat aatcggtacg caatttttgc aggctctctt tcagacttgg gatcaatttg
     7681 tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg acgtggcacg
     7741 ccactttctg caatcgcctg acctactgcg gcttcgttat catagatttg tgcggtatca
     7801 attgcgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac gtagtctttc
     7861 aacctaaacc aaatgcaggg atagccataa tatgcctctt ttaatgctat tttcgttaac
     7921 tgaggtgatt atgggcttca gactgttgag gaaaaaagcg caaaatgcag aggatttttg
     7981 cgattctggc aataatagat atacagacga aaaaaaacct cgagcgttaa ctcgaggctt
     8041 cttaataagt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcagg
//
LOCUS       DADLTD010000178         8011 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLTD010000178 DADLTD010000000
VERSION     DADLTD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.179"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..527)
                     /locus_tag="JN701_003288"
                     /pseudo
     CDS             complement(1..527)
                     /locus_tag="JN701_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            744..2087
                     /locus_tag="JN701_003289"
     CDS             744..2087
                     /locus_tag="JN701_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7365635.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            2312..3967
                     /locus_tag="JN701_003290"
     CDS             2312..3967
                     /locus_tag="JN701_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY7365636.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            4107..4331
                     /locus_tag="JN701_003291"
     CDS             4107..4331
                     /locus_tag="JN701_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY7365637.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            4394..4930
                     /gene="rimL"
                     /locus_tag="JN701_003292"
     CDS             4394..4930
                     /gene="rimL"
                     /locus_tag="JN701_003292"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY7365638.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4925..5905)
                     /locus_tag="JN701_003293"
                     /pseudo
     CDS             complement(4925..5905)
                     /locus_tag="JN701_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
     gene            6029..7021
                     /gene="tehA"
                     /locus_tag="JN701_003294"
     CDS             6029..7021
                     /gene="tehA"
                     /locus_tag="JN701_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY7365639.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIVILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            7018..7611
                     /gene="tehB"
                     /locus_tag="JN701_003295"
     CDS             7018..7611
                     /gene="tehB"
                     /locus_tag="JN701_003295"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY7365640.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            7914..>8011
                     /locus_tag="JN701_003296"
     CDS             7914..>8011
                     /locus_tag="JN701_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY7365641.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSG"
BASE COUNT         2034 a         1869 c         1989 g         2119 t
ORIGIN      
        1 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
       61 accaatgccg atatcgattt ccccggtttt caaacccgcc agaatcatag ggttactcat
      121 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
      181 taatatcccc agtgccgcag taggtagtgc accaatcctg acgacatcat tattaagacc
      241 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
      301 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
      361 gcgagcgccc gtcagctgct acagttcatt caatgtctta gagagcgcag gttgactcaa
      421 attaagggtt tcagccgcgc ccccaaagtt ccttgttgtg cgacagctac gaatgtatga
      481 aggtggcgca aacggatgcg ctgactaaac agactatttt tttccataag cgatgttaaa
      541 aacgaagcgg tgtcgctgac aagtgaagtt gtttgattat gataacttga ttgcaaaata
      601 taattaacaa ttaaagcaat tgtgttacag caaaatggat aatattgatg ttttcgcggc
      661 gagatcacag tttgtaaatt cttcccgcca gagtgaatgc ggttacctac actccagatt
      721 actgaccact ggaggcagac actatggcga acagcatcac ggcggatgag attcgggaac
      781 agttttcgca ggcaatgtca gccatgtacc agcaagaagt tccgcagtac ggcacgctgc
      841 tggaactggt agctgatgtg aatctggctg tgctggaaaa caatcctcaa ctgcacgaaa
      901 aaatggtaaa tgcagatgag ctggcgcgac tgaatgttga acgtcatggg gcgattcgcg
      961 ttgggactgc acaagagctt gctactcttc ggcggatgtt tgccattatg gggatgtacc
     1021 cggtgagcta ttacgatctc tcgcaggcag gggggccggt acattcgaca gcatttcggc
     1081 ccattgatga tgcttctctg gcgcgtaatc ccttccgcgt ttttacctcc ttactccgcc
     1141 ttgagcttat cgagaacgaa attttgcgcc agaaagcggc ggagattctg cgtcagcgcg
     1201 atatcttcac cccacgttgt cgacaactgt tagaggaata tgagcagcag ggcggtttta
     1261 acgaaacaca ggcacaggaa tttgtgcaag aggccctgga aacgtttcgc tggcaccagt
     1321 cagcaacggt agatgaagaa acctatcgcg ccttgcacaa cgaatatcgg ttgattgctg
     1381 atgtggtctg ttttcctgga tgccatatca accacctgac gccacgtacg ctggatattg
     1441 accgggtgca gtcgatgatg cctgaatgcg gaattgaacc caaaattctc atcgaggggc
     1501 cgccgcgccg cgaggtaccg attttactac gccagaccag ctttaaagca ctggaagaga
     1561 cggtgttgtt tgcggggcag aaacagggca cgcataccgc gcgctttggt gaaattgagc
     1621 agcgtggcgt ggcattaacg ccgaaagggc gacaactgta tgatgatctt ctgcgtaacg
     1681 ctggaaccgg gcaggataat ctcactcacc aaatgcattt acaggaaacc ttccgcactt
     1741 ttcctgacag tgagttttta atgcgtcagc aagggttggc atggttccgg taccgtctga
     1801 cgccttcggg tgaggcgcat cgtcaggcga ttcatcttgg agacgatcca cagcctttaa
     1861 ttgaacgtgg ttgggtagtg gcgcaaccca tcacctatga agatttcttg cccgttagcg
     1921 cggcggggat cttccagtca aatctgggta atgaaacgca gacacgcagt cacggtaatg
     1981 ccagtcgcga agcatttgag caggcgttgg gttgtccggt tttggatgag ttccagcttt
     2041 accaggaagc ggaagaacgc agtaaacgtc gctgtggttt gctttaaatt ctgaccatcc
     2101 gcctttgcaa aaatttgcct gattttacaa acgaatcagg ctcatcccat cgacataaaa
     2161 aaaatgccga tttatgcata ttctctcagt tcaacaattg gattattaat aaatattgtc
     2221 tagagtgagc ggtcataaat aagcactttc ttgccgctga aaacgaccag cgcgggacca
     2281 ttcacaacac cagaaggact cactttcagg tatggatcgt agacgattta ttaaaggttc
     2341 aatggctatg gccgccgtgt gcggtaccag cggcattgct tctctttttt ctcaggcggc
     2401 attcgcggca gattctgata ttgccgacgg gcaaacccag cgttttgact tctccattct
     2461 acagtcaatg gcgcacgact tagcgcaaac agcgtggcgt ggtgcgcctc gtccgttacc
     2521 tgacacgctg gcgacaatga cgccgcaggc ttataacagt attcaatacg acgccgaaaa
     2581 atcgctctgg cataacgttg agaaccgtca actggacgct cagttcttcc atatgggaat
     2641 gggattccgt cgccgcgttc gtatgttttc tgtagatcca gcaacacatc tggcgcgtga
     2701 aattcacttt cgcccggagt tgttcaaata caacgatgcg ggtgttgata caaaacaatt
     2761 agaagggcaa agcgatctcg gctttgccgg ttttcgcgtg tttaaagccc ccgaactggc
     2821 gcgccgtgat gtagtatcat ttctcggcgc gagttatttc cgcgccgttg atgatacata
     2881 tcaatacggt ttgtcggccc gcggcctggc gatcgacact tacaccgaca gtaaagaaga
     2941 gttccccgac tttaccgcct tctggtttga tacggtaaaa ccgggggcaa ctacctttac
     3001 cgtttatgcg ttgctcgata gcgccagcat tactggtgcc tataagttca ctatccattg
     3061 tgagaaaagt caggtgatta tggatgtgga aaatcacctg tatgcgcgca aagacattaa
     3121 acagctgggc attgcgccga tgaccagtat gttcagctgc ggtactaatg aacgtcggat
     3181 gtgcgataca attcatccgc aaattcatga ctctgatcgt ctgtccatgt ggcggggcaa
     3241 cggcgagtgg atttgccgtc cgctgaataa tccgcaaaaa ttgcagttca atgcttacac
     3301 cgacaacaac ccgaaagggt ttggtttatt gcaactggat cgtgacttct cccattatca
     3361 ggacattatg ggctggtata acaaacgccc aagtctgtgg gtggaaccgc gtaacaagtg
     3421 gggtaagggc accatcggcc tgatggaaat cccaacaacg ggcgaaacgc tggataacat
     3481 tgtctgtttc tggcagccag aaaaagctgt aaaagcaggt gatgagtttg cattccagta
     3541 tcgtctgtac tggagtgcgc aaccgcctgt tcattgccca ttagcgcgcg ttatggcgac
     3601 gcgtaccggc atgggcggtt tcccggaagg ttgggcgcca ggtgaacact atcccgaaaa
     3661 atgggcgcgt cgttttgccg tcgatttcgt tggtggtgat ctgaaagctg ccgcgccaaa
     3721 aggcattgag ccggtgatta cgctttccag tggggaagcg aagcaaatcg aaattctcta
     3781 tattgaaccc atcgatggtt atcgtattca gtttgactgg tatccgactt cggactccac
     3841 tgatccggtc gatatgcgga tgtatctacg ttgtcagggg gacgctatca gtgaaacatg
     3901 gctgtatcag tatttcccgc cagcgccgga taaacgtcag tatgttgacg accgcgtgat
     3961 gagttaatcg ttttttcttc ggcaccttct tcgggaggtg ccgtctggtt aaacacgatc
     4021 ccgctcgcat ttttccctaa gttaaatgag taatctgatg gtgtgtattt cagatacacc
     4081 ttgtcagcca ctaacaggga gtgcgtatgt ttccagaata ccgagattta atatcccgtc
     4141 tgaaaaacga aaatcctcgc tttatgtcct tgttcgataa acacaataaa cttgatcatg
     4201 aaattgccag aaaggaaggt ttcgacggtc gagggtacaa tgcggaagtg gtccgcatga
     4261 aaaaacaaaa gctacagtta aaagatgaga tgctcaaaat cctgcagcag gagagcgtca
     4321 aaggggtgta aactttccta agccgccagc caggcggctt ttttaacaac agcatggatt
     4381 gactggagat aagatgactg aaacgataaa agtaagcgaa tcacttgaat tacatgctgt
     4441 tgcggaaagt cacgtcacac ctctttatca gttaatctgt aaaaataaaa cctggttaca
     4501 gcagtcgcta aactggccgc agttcgttca gagtgaagag gacacgcgaa aaacggtgca
     4561 gggtaatgtg atgttgcatc aacgcggcta tgccaaaatg ttcatgattt tcaaagaaga
     4621 tgaacttatc ggcgttatct cgtttaatcg tattgaacca ctgaataaaa ctgctgaaat
     4681 cggctactgg ctggacgaat ctcatcaggg gcaggggatc atttctcagg cgctgcaggc
     4741 attgattcat cattacgctc agtctggtga acttagacgc ttcgtgatca aatgtcgggt
     4801 ggacaatccg caaagcaacc aggtcgcttt gcgcaatggt tttatccttg aaggttgcct
     4861 gaaacaggct gagttcctga atgatgccta tgatgatgtg aacttatacg cgcgtattat
     4921 cgattcataa taaccctgcc agcggcgttc gcgtaatgcg ctcctcgccg ttaatgactt
     4981 ttggaccacg gacatgaacc gtgtcgccat caaaggcttc aacgacggcg tggtcggtta
     5041 actccacatg gttttcaatt atcaccgcac ctgaaatacg gctctcacct tgtattacaa
     5101 cgtgctcgtc gagcagaatc ggttcaccac gtaccacggc atttccgcca atcaagacat
     5161 gatgttttaa cacacaatta ccttccacaa tggcatattc cgccacctgc gaactgtaat
     5221 gaatcgtcgg aatggcatct tcttctatgc cagctttcac ctgcgcgtga ccgtagactt
     5281 tagcgcaatc gcatagccag acgttgttct cttcattacc ttcaacactg gcaaaatcaa
     5341 aaacttcggc gcgatgttca ataaaagcat accggacgac ggcatcgcca taaatttgtg
     5401 cctggtggac aatacgcgag gcgctaaccc ttgcgcgatc ataaatttgg agcaggagtt
     5461 gatggtcggg cgttaagcct tgtgcggcga cgatcataga atgttgatca attaaggcgt
     5521 gaccaaatat tctacattgc ccgtaaacta atgagtcatg aatggtgaca ctgtcactta
     5581 tatatgcacc ctgactgatt tcactattat cgatccagac gttatccgtt gcgtaaacct
     5641 ctccccacag cacgctggtg ccggtgatac gcgtattacc agaaatcacc gttccgccga
     5701 acgcaatagc attctggtcg taaatccagc aatttccttc ctatgctaac accgtttcgc
     5761 gatcgatcca gccgccagcg gttcctgcta tcacatcgtt aaaatcgcta atggcgataa
     5821 tctgccgtaa taacacactt tttttagtgc catcttcctg ataactaaag gctcgctgtt
     5881 cttcacttag acgatatttg cgcatagcgt tttctcacag gtgacttact atgaccgtag
     5941 caaattctgc ggctctggct atgctcgaga aaattccata aaatgcattt caaatatact
     6001 ttataaatta aacaaaatga gtaagaagat gcagagcgat aaagtgctca atttgccggc
     6061 aggctacttt ggtattgtgt tggggacgat agggatggga tttgcctggc gctatgccag
     6121 ccaggtttgg caggtcagcc actggttagg ggatgggctg gtgattctgg cgatgatcat
     6181 ctggggatta ttaactagcg catttattac ccgactcata cgctttccgc atagcgtgct
     6241 ggcggaagtt cgccatccag tgctgagcag ttttgtgagt ttgtttccgg caacgacgat
     6301 gctggtggcg attggttttg ttccgtggtt tcgcccactg gcggtgtgcc tgttcagttt
     6361 tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact gcgggattat ggcgcggatc
     6421 tcaccctgaa gaagctacca tgcctggact gtatctgccg acagttgcca acaactttat
     6481 cagcgcaatg gcctgtggtg cgttgggcta caccgacgcc ggtctggtgt ttttaggcgc
     6541 aggcgttttc tcatggctaa gccttgaacc ggtgatcttg cagcgtctgc gcagttcggg
     6601 agaattaccc acggcactgc ggacatcact cggcattcag ctcgctcctg cgctggtggc
     6661 ctgtagtgcc tggttgagcg tcaacggcgg cgagggtgat acgctggcga aaatgctttt
     6721 tggttatgga ctgctgcaac tgctgtttat gctacgtctg atgccatggt atctctccca
     6781 gccatttaat gcttcattct ggagtttctc gttcggcgta tctgcactgg caaccaccgg
     6841 tttgcatctg gggagtggca gcgaaaatgg atttttccat acgctggcgg taccgctgtt
     6901 tatctttacc aattttatta ttgtaatact gctcatccgt acttttgcgc ttctgatgca
     6961 gggaaaattg ttagtcagaa ccgagcgcgc tgttttaatg aaagcagagg acaaagaatg
     7021 atcattcgtg acgaaaacta ttttactgat aaatatgaat taacccgcac acactctgaa
     7081 gtactggaag cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg ctgtggcaat
     7141 ggtcgtaaca gtctttacct ggcagccaat ggttatgatg ttgacgcatg ggataaaaat
     7201 gccatgagta tcgccaatgt agagcgcatt aaatccattg aaaatctgga taatttacac
     7261 acccgagtcg ttgatctgaa taacctcaca tttgatagac agtacgattt tattctttcg
     7321 actgtggtac tgatgttcct tgaggctaaa accatcccag ggctgattgc caatatgcaa
     7381 cgttgcacta aacttggtgg ttacaacctg attgtggcgg cgatggatac cgctgattat
     7441 ccatgtaccg tcggcttccc gtttgccttc aaagagggag aattacgtcg atattacgaa
     7501 ggctgggaga gggtgaaata caatgaagac gtcggcgagc tgcaccgcac cgacgccaac
     7561 ggtaatcgta ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg acccgctaag
     7621 aataaggcgc gtgaaaattc cccacgctga gatgatttac tgttcttctt ttcggtaagc
     7681 atatttttta tcgaagggat gtgaaattaa tcacagtagt cgaagttttt tagcagctta
     7741 acttactgaa atttaactac tgatgattga cttagcccct ttttcggcat tgactatgtc
     7801 gtctgaaaag gggctgaaaa atttattttc accaacactt tttttgccac aacacgaagc
     7861 ggcgcttttt gctataactt aaaaagtaat ataatcatct caggaaacta ttcatgcgta
     7921 ccacatcatt tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct ggttgtgcct
     7981 ctaaaatcac ccagccagat aaatattctg g
//
LOCUS       DADLTD010000179         7889 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLTD010000179 DADLTD010000000
VERSION     DADLTD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.180"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            47..826
                     /gene="srlD"
                     /locus_tag="JN701_003297"
     CDS             47..826
                     /gene="srlD"
                     /locus_tag="JN701_003297"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY7365642.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLVEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            931..1289
                     /gene="gutM"
                     /locus_tag="JN701_003298"
                     /pseudo
     CDS             931..1289
                     /gene="gutM"
                     /locus_tag="JN701_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            1356..2129
                     /gene="srlR"
                     /locus_tag="JN701_003299"
                     /gene_synonym="gutR"
     CDS             1356..2129
                     /gene="srlR"
                     /locus_tag="JN701_003299"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY7365643.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPVFRQALEEKGIDVIITGESNE"
     gene            2122..3087
                     /gene="gutQ"
                     /locus_tag="JN701_003300"
     CDS             2122..3087
                     /gene="gutQ"
                     /locus_tag="JN701_003300"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY7365644.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(3084..4598)
                     /gene="norR"
                     /locus_tag="JN701_003301"
     CDS             complement(3084..4598)
                     /gene="norR"
                     /locus_tag="JN701_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY7365645.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            4785..6224
                     /gene="norV"
                     /locus_tag="JN701_003302"
     CDS             4785..6224
                     /gene="norV"
                     /locus_tag="JN701_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY7365646.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            6221..7354
                     /gene="norW"
                     /locus_tag="JN701_003303"
                     /pseudo
     CDS             6221..7354
                     /gene="norW"
                     /locus_tag="JN701_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(7482..>7889)
                     /locus_tag="JN701_003304"
     CDS             complement(7482..>7889)
                     /locus_tag="JN701_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="carbamoyltransferase; involved in the formation of
                     the cyanate group of hydrogenase; catalyzes the formation
                     of carbamoyladenylate from carbamoylphosphate and then
                     transfers the carbamoyl group to HypE; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7365647.1"
                     /translation="GEAACALEALAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQA
                     QVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTL
                     LFPQSLPAGDGGLSLGQGVIAAARWLAGEVQNG"
BASE COUNT         1932 a         2126 c         2100 g         1731 t
ORIGIN      
        1 cgcgccggga tcggttttga agtttgaatc tgttaaggag taaaaaatga atcaggttgc
       61 cgttgtcatc ggtggtgggc aaaccttagg cgcgttcctg tgccacggtc tggttgccga
      121 ggggtatcgc gtcgcggttg tcgatattca gagcgacaaa gccgcaaatg tggcacaaga
      181 aattaacgcc gaatatggtg aaagtatggc gtacggtttt ggtgctgacg ccactagcga
      241 gcaaagcgtt ctggcgctct ctcgtggggt agatgaaatc tttggtcgcg tggatttgct
      301 ggtctatagc gccggaatag caaaagcagc ctttatcagc gacttccagc ttggcgattt
      361 tgaccgttcg ctacaagtga atctggtggg ttatttcctg tgtgcccgtg aattttcgcg
      421 tttgatgatc cgcgacggga ttcagggtcg cattattcag atcaactcga aatccggcaa
      481 agtgggcagc aaacacaact ctggctacag cgcagcgaaa tttggtggcg tcgggctgac
      541 tcaatcactg gcgctggatc tggtggagta cggcattacg gtgcattcac tgatgctcgg
      601 taacctgctg aaatcgccga tgttccagtc actgttgcca caatacgcga ccaagctggg
      661 tatcaaaccg gatcaagtcg agcagtatta cattgacaaa gtaccgctca aacgcggctg
      721 cgattatcaa gatgtgctga atatgctgct gttctacgcc agtcctaagg cgtcgtactg
      781 caccggacag tcgatcaatg tcaccggcgg tcaggtgatg ttctgatcaa cagcggagat
      841 ccattaagga tctccgtgag actatagaat gcctgatgcg ctacgctcat caggcataca
      901 ggacttccgc cactacatta aggaaaagtt atggtatccg cactcatcac cgtcgccgtt
      961 atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt taaccgtgcc
     1021 ttcgacacac tatgccagca agggcgggtt ggcgtgggtc gttccagcgg gcgctttaaa
     1081 ccgcgggtcg tggtcgccat cggctggacg atcagcagcg catcgtcgac accttgttta
     1141 tgaaaggact gaccgtcttc gcccgaccgc aaaaaattcc cgcaattacc ggtatgcatg
     1201 cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
     1261 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt
     1321 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg
     1381 ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg gcgcaatact
     1441 ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat gccggaaccg
     1501 tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg cctatcgatc
     1561 ataaaacact catcaacacc cacaagaaag agctgattgc agaagctgcc gttagtttta
     1621 tccatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag atggttcccc
     1681 tgctctcgcg ctttaataac atcacggtga tgaccaacag cctgcatatc gtcaatgcgc
     1741 tatccgaact ggataacgaa caaactatcc tgatgccagg cggaacgttt cgtaaaaaat
     1801 cggcctcatt tcacggacag ctggcagaga atgccttcga acatttcagt ttcgataaat
     1861 tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccaccttc aacgaggttt
     1921 ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg gcggactcat
     1981 caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc gataagctga
     2041 ttaccgacgc aggtatcgat ccggtgtttc gtcaggcgct ggaagagaaa gggatcgatg
     2101 tgatcataac cggagagagc aatgagtgaa gcactactga acgcgggacg tcagacgtta
     2161 atgctggagt tgcaggaagc aagccgttta ccggaacgtc tgggcgatga ttttgttcgc
     2221 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
     2281 ggccacattg gtaagaaaat cgccgcaaca cttgccagta ccggcacacc ggcttttttt
     2341 gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg cgatgtgatg
     2401 ctgtttatct cttactccgg tggcgcgaag gaactggatc tgattattcc gcgtctggaa
     2461 gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct gggcctggcg
     2521 gcaaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg
     2581 acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc ggtcatgcag
     2641 gcgcgcggat ttaatgaaga agattttgcc cgctcccacc cagccggggc actgggcgct
     2701 cgcttgctga ataaagtgca tcatctgatg cgccgtgacg atgccatccc acaggtggcg
     2761 ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc gcaccggtct ggggctggtg
     2821 gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta ccgacggcga tttacgtcgc
     2881 tggctggtag gcggcggcgc actcaccacg ccagttaatg aagcgatgac gacaggcggc
     2941 accacgttac aggcgcaaag tcgcgccatc gacgccaaag agatcctgat gaagcgcaaa
     3001 atcactgccg caccggtggt ggatgaaaac ggcaaactca ccggcgcaat taacctgcag
     3061 gatttctatc aggccgggat tatttaatcc ttcattccca gacgtttcgc cagccgatgc
     3121 aggttggcga cgtcggtttc cagcatccgc gcgcaggcag cccagttatg atgattttgt
     3181 gccagtgcct ggcgaatagt ttcacgctgg aacgcttctg tcgcttcacg caggttttgc
     3241 ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa
     3301 tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg ctctcgccag cactaccgcc
     3361 cgatgaatag catgttccag ttcgcgcacg ttccccggaa aacgatagtg ttgcagtaaa
     3421 tttcgcgctc cggcacttaa taccacgcgg gagagcccca gccgcaaacg acactgttcg
     3481 cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcaccgaa
     3541 agtggaaaca cgctcaggcg atgaaacaag tcagcgcgaa atcgccctgc cagcacctct
     3601 tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat cgacccgcaa actacggtca
     3661 tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc
     3721 aacgacaact cgccgatctc atccagaaac agagtgccgt tatccgccat ttcaaacttc
     3781 ccgctgcggt tactgatagc gccagtaaac gctcctttca catgcccgaa caactcactt
     3841 tccgccacac tttccggcag tgcagcacag ttgagataga ccagcggatt caccgcccgt
     3901 ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgctg
     3961 atcaggacgt tgagatcgga cgccgccaca atctcaatct cttttttcag ttgcgtcatg
     4021 ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt caaacggcgt ggcatcgcct
     4081 ggcatcatat tctggctttc cagttgttca atcagcaacg cattgcttaa cgctcccgcc
     4141 gccagcgcag caatcagccg tagctcttcg tcgctgaaaa catcgaactg atcgggctgc
     4201 atcccgtcga gcgtcagtgc gccgatcagg ttttgcccgg caaacaatgg cagaccaacg
     4261 caggcgtgaa ccttcagact ctcctgccca ggaatcaaac cgtcataggg atcgggcaat
     4321 tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg caatcgcttc cagccgtgga
     4381 tgcccttcca gcgcaaagcg tctaccgagt acaccctttg ccagaccgtc gatggcaagc
     4441 ggaataaact gccgcgaatc gtaacgtagc aacgcagacg catcgcactc cagcacctga
     4501 cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg
     4561 atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga
     4621 ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact cattaatgag
     4681 cataatttta tttatagagt aaaaacaatc agataaaaaa ctggcacgta atctgcaatt
     4741 agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga
     4801 aaaataacat tcattgggtt ggtcaacgtg actgggaagt gcgtgatttt cacggtacgg
     4861 aatataaaac gctgcgcggc agcagttaca acagctacct catccgcgaa gaaaaaaacg
     4921 tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg
     4981 aaatcgatct ggcggatatc gattacatcg tgattaacca tgcagaagag gaccacgctg
     5041 gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc gatttactgt actgccaacg
     5101 ctatcgactc gataaatggt catcaccatc atccggagtg gaattttaat gtggtgaaaa
     5161 ctggtgacac gctggatatc ggcaacggca aacagctcat ttttgtcgaa acgccaatgc
     5221 tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtgctg ttcagtaacg
     5281 atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagacgg
     5341 agcttttcga gcagtgccag cgttactacg ccaatatcct gacgccgttc agccgcctgg
     5401 taacaccgaa aattaccgag atcctgggct ttaacttacc agtcgatatg atagcgacct
     5461 ctcacggcgt ggtatggcgc gataacccga cgcaaattgt cgagctgtac ctgaaatggg
     5521 cggctgatta tcaggaagac agaatcacca ttttctacga caccatgtcg aataacaccc
     5581 gcatgatggc tgacgctatc gcccagggga ttgcggaaac cgacccacgc gtggcggtga
     5641 aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa
     5701 aaggcgtgct ggtcggcact tcgacgatga ataacgtgat gatgccgaaa atcgccgggc
     5761 tggtggagga gatgactggt ttacgcttcc gtaacaaacg cgccagtgct ttcggctctc
     5821 acggctggag cggcggtgcg gtggatcgtc tttccacgcg cctgcaggat gcgggtttcg
     5881 aaatgtcgct tagcctgaaa gcgaaatggc gaccagacca ggacgctctg gagttatgcc
     5941 gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg cagagcacgg
     6001 tgaataaggt agtcaaagaa gaaacctctg ccaccacgac ggctgacctc ggcccacgga
     6061 tgcagtgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca atgcaggacg
     6121 ttgcgccagg aacgccgtgg agtgaagtcc cggataactt cctctgcccg gaatgctccc
     6181 tcggcaaaga cgtctttgaa gaactggcat cggaggcaaa atgagtaacg gcattgtgat
     6241 catcggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac aggacgccac
     6301 tattccatta accctgattg ccgccgacag catggatgag tacaacaaac ctgacctcag
     6361 ccatgttatc agtcaggggc aacgtgccga tgaccttacc cgccagacgg cgggtgaatt
     6421 tgccgagcag tttaatctgc acctgtttcc acaaacctgg gtgacggata tcgatgccga
     6481 agcccgtgtg gtgaaaagcc agaataatca gtggcaatac gacaagctag tactggcaac
     6541 cggtgccagt gcctttgtcc cgcctgtgcc tgggcgtgag ttaatgctga cgttaaatag
     6601 tcagcaagag tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg tgttgattgt
     6661 tggcggtggt ttgattggta gcgaactggc gatggatttt tgtcgtgcag gcaaagcggt
     6721 cacgctaatc gacaacgctg ccagtattct ggcgtcgtta atgccaccgg aagtaagcag
     6781 ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttgaaat ctcagttaca
     6841 ggggctggaa aaaacggatt ctggcattct ggcaacgctg gaccaccagc gtagtatcga
     6901 agtggatgcg gtaattgccg ccaccggact gcgcccggaa acagccctgg cacgacgcgc
     6961 cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca gtaatgacga
     7021 tatttatgtg ctgggcgatt acgcggaaat caacggtcag gtactgccat tcctgcagcc
     7081 gattcaactt agcgcgatgg tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa
     7141 actaccgacg atgctggtga aaatcaaaac gccggaatta ccgctgcatc tggcaggcga
     7201 aacccagcgt caggatttac gctggcaaat taataccgaa cgctagggaa tggtggctcg
     7261 cggtgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga
     7321 ggcatttgga ttgttgaaaa cattgccgat gtaggtgggc tactgtgcct aaaatgtcgg
     7381 atgcgacgct agcgcgtctt atccgaccta cggggacgca tgtgtaggcc ggataaggcg
     7441 tttacgccgc atccggcaat ggtgtccaaa tgcaacacgt tttatccgtt ctggacttca
     7501 cccgctaacc aacgcgccgc agcaataacc ccctgcccca gagacaaacc gccatcaccc
     7561 gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga gataatgcgc cagacgtgca
     7621 cgcagcaaac ggttatgaat aaccccgccg ctaaatacca gcgtagtgat accacgcatc
     7681 gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca gcgcatcatg aaacgcccac
     7741 gcgcgttgat taacctgtgc ctgccagttc agccactgct gccagaaagc ggcgagatcc
     7801 agttgattgt ccaccagcgg catcgtcacc ggatgcgtca ctccgtggca tgaggctgcg
     7861 agcgcctcca gagcacaagc cgcttcacc
//
LOCUS       DADLTD010000180         7827 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLTD010000180 DADLTD010000000
VERSION     DADLTD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.181"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            636..902
                     /locus_tag="JN701_003305"
     CDS             636..902
                     /locus_tag="JN701_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7365648.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            899..1498
                     /locus_tag="JN701_003306"
     CDS             899..1498
                     /locus_tag="JN701_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY7365649.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1491..1778
                     /locus_tag="JN701_003307"
     CDS             1491..1778
                     /locus_tag="JN701_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HAY7365650.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1771..2226
                     /locus_tag="JN701_003308"
     CDS             1771..2226
                     /locus_tag="JN701_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365651.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            2362..2682
                     /locus_tag="JN701_003309"
     CDS             2362..2682
                     /locus_tag="JN701_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HAY7365652.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            2697..5030
                     /locus_tag="JN701_003310"
     CDS             2697..5030
                     /locus_tag="JN701_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7365653.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDVAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEVTRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPLDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            5377..5571
                     /locus_tag="JN701_003311"
     CDS             5377..5571
                     /locus_tag="JN701_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7365654.1"
                     /translation="MWIKDALVWIRATDPNLLIKNYLTFRFIDGLKIDWSEKQWGHKR
                     GHISLLCFIICFLSLTYFDI"
     gene            5789..7057
                     /locus_tag="JN701_003312"
     CDS             5789..7057
                     /locus_tag="JN701_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7365655.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            7448..>7827
                     /locus_tag="JN701_003313"
     CDS             7448..>7827
                     /locus_tag="JN701_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365656.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LKLSISKYIENAHKNIIDNDELKH"
BASE COUNT         1920 a         2021 c         2061 g         1825 t
ORIGIN      
        1 ggcgttctca aggtgggcgg ctctggcctt ttccagttgt gccagtgcgg gttgcagata
       61 ttcagggatg gtgttgtcag acattttccg gctcctcgtc acttcaggtt aagaaaattg
      121 tgacgtacac cggacaacaa cacgacgcat tgcagatgtg ccagccctga cacaggagac
      181 tcatcctcag accggcaagc caggaaaagg tcgcaggaaa aaccggctta ctgtttgttt
      241 ttttatattt tactgttcac ctctgttcac cataagaaaa aagataagta atacagtaag
      301 ttaaagggtg aacaatcgca gtaatgactg ttcaccgtct gttcaccact gttcacccgc
      361 tcatagactt tttgtgctgt ttattacttt ttatttttat taatccactg aaataaataa
      421 gaaaaaacaa tttgtatttc actataattt tttccagctc cttccagagc gttttaaagc
      481 catctggcgc tgatgtataa aaaatacaca gccattgtaa gactgccgga acaaatcccc
      541 cctgttgcat ctgccaaaaa tattcacaaa ataaagcgct acccgaagcc ggacggactt
      601 atccggtgct gtatggacat taacgaggta gcccgatgca agctgttttt tcttccccgt
      661 ctcccgcccc tgtgacgcca ctgatgccgc tgccggacat cacgcaggag cgttttttac
      721 gtctgccgga agtgatgcac ctgtgcggcc tgtcacgctc gaccatctat gaactcatcc
      781 gtaaggggga atttccgccg caggtgagtc ttggcggtaa aaatgtggcc tggctgcact
      841 ctgaagtcac cgcatggatg gccggacgca ttgccggacg caaacggggg tacgacgcat
      901 gatgatgccc gctctgcaaa aactcccttt ttctggcttg cctttttccg gcatttgcgg
      961 atatagtttt tccgctgccg caaaatcggc agccgggcgt gagaacccga gcaattcaca
     1021 ggcgacatat gacgcgccac gcgtcttttt ttgtgtcgca atcaatgcca cagagcgcca
     1081 gattatggtg tggtgtgtgg tcagtcgtgc agatggcatt ccgtgcatcc tgcccgcgtc
     1141 cgctcattat gccgcagagt caatggtagc tcaggcgggg cagcctccgg gctggccggt
     1201 atcctgtgaa gccggtattc tcacccccgt ctgggctatc gccatcgagc gtgagaactc
     1261 cggcgatagc gttatttgct attcacagga ggctgccatc atggctacaa cccttacccc
     1321 ctcacaccct gaatttgtct ttgtgtttgc ggctgtccgt cgcgcagacc gtcatccccg
     1381 tatctgtatg cttcgcaccg tggccggtga tgaacgcagt gcccgccgtt cccttgtccg
     1441 tgactatgtg ctctcccttg ctgcccgtct gccggtggtg gaggtgtccc gtgcgtaata
     1501 aaaaagcccc tcagaccgtc tcagcacgtc atgacgcccg tgaacacctc agcattgagg
     1561 cttaccataa gctcaaccgc gccagcgccg tatcccagtt tgttgggggt gatttgattc
     1621 accgtgaact ctccggcctg catcagctct acattccgca tattttcagc tacctgaatg
     1681 aagatattga ttttgtgctg aatgagctga aagccaaagg cctgtgccgc gattttctcg
     1741 cccagcagaa agaccgggga gacaggacgc atgtttgatt ttccccagcc cggtgagatt
     1801 taccgttctg ccggttttcc cgatgtggcc gtggtcggca ttctggaaga cggtattccg
     1861 tgggaaatgc cgtaccgctg cccggacatt gtctggaacc cgtaccgccg taaattcagt
     1921 atccttgtgc gtatcctcgc cgacgggcgc accacagaca tcccgctggg gcgttttctg
     1981 cgggaattta cctgtgaccg tcctgacctg tttaaacgca gccccgtaaa ccggcatgcg
     2041 gtactgaaag aaatggccgg agacccggaa ttacagaaat ggcgggagaa atatctggat
     2101 atttacccgc aggacactgt tccggtcagc cgggcagcac cggtagcgcg ggaatggcgg
     2161 gaaattcccc gcacggagcc tgacccggaa atcaccccgg ataacagtta ccgcaattat
     2221 ctgtaattaa aaaacgacac ccgaaaaatt aaatgtgcgt attcgcgcag ggatacgcac
     2281 gtcttcagga gacgcagata tgccttatca gttaatgcaa ccggcacgga atgcagtcat
     2341 ctgtcacagg gaggaaagca aatgaaaaca cccttaccgc ccgtcttacg cgcagccctt
     2401 taccgtcgtg ctgtcgcctg tgcctggctg accgtgtgcg aacgtcagca ccgctacccg
     2461 catctcaccc ttgagtcact ggaggcggcc atcgccgctg agctggaagg cttttatctg
     2521 cgccagcacg gtgaggaaaa agggcgtcag atagcctgtg ccctgctgga agatttaatg
     2581 gaatccggcc ccctgaaggc cgcaccgtcg ctgtcctttc tcgggctggt tgtgatggat
     2641 gaactctgtg cccgtcacat aaaagcgccg gtactgcact gaaggagaac aacaccatga
     2701 aaatgaacgt aaccgccacc gtcagccatg cgctcggcca ctggccgcgt attctcccgg
     2761 cgctggggat tcaggtgctg aagaaccatc atcagccctg tccggtctgt ggcgggagtg
     2821 accgcttccg ttttgatgac agggaggggc gcggcacctg gtactgcaat cagtgtggtg
     2881 ccggtgacgg cctgaaactg gttgaaaagg tgtttggtgt ctccccgtcc gacgtggccg
     2941 caaaggtggc tgccgtgacc ggcagtctgc ccccggctga cccggcagtg acggccgccg
     3001 ccgttgctga aacagacgct gcccggaaga acgccgccgc actggcacaa accctgatgg
     3061 caaaaacccg tcccggaacc ggtaacgcct acctgacccg caagggcttt cccggccggg
     3121 aatgccggat gctgaccggc acacacagag ccggtggcgt gagctggcgc gccggtgacc
     3181 ttgtggtgcc actgtatgac gacagcggcg aactggttaa ccttcagtta atcagtgctg
     3241 acggccgtaa gcgcaccctg aaaggcggac aggtcagggg cacctgtcac acccttgaag
     3301 gacagaatca ggccggaaaa cgtctgtgga tagcggaggg atacgcgacc gcacttaccg
     3361 tacatcacct gaccggtgaa acggtgatgg tggcgctttc ttccgtgaac ctcctttctc
     3421 tggccagcct tgcccggcag aagcatccgg cctgtcagat tgtccttgcc gcagaccgtg
     3481 acctcagcgg tgacggccag aaaaaagccg ccgcagccgc agatgcgtgt gaaggtgttg
     3541 ttgccctgcc gccggtcttc ggtgactgga atgatgcctt cacgcagtac ggcggggagg
     3601 tcacccgtaa ggccatttac gatgccatcc ggccaccggc tgaaagcccg ttcgacacca
     3661 tgagcgaagc agagttttcc gccatgagta ccagcgaaaa ggccatgcgt atctatgagc
     3721 attacggcga ggcgctcgcg gtcgatgcca acggccagct tctgtcccgc tatgaaaatg
     3781 gtgtctggaa ggtgctgccg ccactggact ttgcccggga tgtggccggg ctgtttcagc
     3841 gtctgcgcgc gccgttctcc tccgggaagg tggcctccgt ggtggacacc ctgaagctga
     3901 ttattccgca gcaggaagcc ccctcccgcc gcctgattgg ctttcgtaac ggtgtgctcg
     3961 acacacagaa cggcacgttc cacccgcaca gtccgtcaca ctggatgcgt accctgtgcg
     4021 atgtggattt caccccaccg gtggaagggg aaacgctgga aacccacgcc cccgcgttct
     4081 ggcgctggct tgaccgtgcc gccggtggcc gtgcggaaaa acgcgacgtg attctggccg
     4141 cactgtttat ggtgctggca aaccgctacg actggcagct ctttctggag gtgaccggtc
     4201 ccggcggcag cggcaaaagt atcatggccg aaatcgccac cctgctggcc ggggaggata
     4261 acgccacgtc ggccaccatt gagacgctgg aatccccgcg tgaacgtgcc gcgttaactg
     4321 gcttctcact gatacgcctg ccggaccagg aaaaatggag cggcgacggt gccggactca
     4381 aggccatcac cggcggcgat gcggtgtccg tggacccgaa ataccgggat gcgtactcca
     4441 cgcatatccc ggcggtgatt ctggccgtga acaataaccc gatgcgcttc accgaccgca
     4501 gcggcggcgt gtcacgccgg cgggtgatta ttcacttccc ggaacagata gccccgcagg
     4561 agcgcgaccc gcagcttaag gacaaaatca cccgcgagct ggcggtcatc gtgcgtcacc
     4621 tgatgcagaa gttcagcgac ccgatgctcg cccggtcact gcttcagtcc cagcagaact
     4681 cagacgaggc gctgaacatc aaacgggatg ccgacccgac gtttgatttt atcggctatc
     4741 tggaaaccct gccgcagacc agcggcatgt atatggggaa cgccagtatc atcccgcgta
     4801 attaccgtaa atacctctat cacgcctatc tggcctacat ggaggcaaac ggctaccgga
     4861 atgtactcag tctgaaaatg ttcgggctgg ggctgccggt gatgctgaag gaatacggac
     4921 tgaattacga gaagcgccat accaaacagg ggatacagac caacctgaca ctgaaagagg
     4981 aaagctacgg cgactggctg ccaaaatgtg acgaccctgc aacaacctga cccacctgac
     5041 cggcatctgc cggtcttttt ttatcccgac ctcccccgaa ggtgaacaat ccactgttca
     5101 cccttcaccg tatattcacc cgttatcaca ctgaaattaa aagagaaaaa tgaaaggtga
     5161 acagtgtgaa caatcaaatc aaaaaaaact tttttctccc tgtgcgattt tcagtacggg
     5221 ggattaatca ccggtatgag tcacaccggc agaatgccgg aggtgaagaa tcgaatgttc
     5281 acccttcacc cattattcac cacctatcac tctgaaataa aaggagaaaa ttaaatggtg
     5341 aacagtgtga acagttcttt cgaaaaaaaa ttttttatgt ggataaaaga tgcactggtt
     5401 tggatccggg ctacggatcc aaacttactg attaaaaatt atttaacttt ccgatttatt
     5461 gatgggctga aaatagattg gtcagaaaaa cagtgggggc acaaaagggg gcatatttca
     5521 ttgttgtgtt ttattatttg ttttttatca ttaacttatt ttgatatttg agtccggcct
     5581 tcgcaccaaa agtatgtaaa tagacctcaa ctgaggtctt tttttatgcc tgaaatccgc
     5641 gctacacaag gcttttccag cttttcccct caactgaagt caacctgact caacccacat
     5701 caacaggctt gtgagtatac taatgagtat atttgccgat tcgatattgc ttgtatactc
     5761 acgagatacc aatggaagga agaccattat ggccctaacg gatactaaag ttcgctctgc
     5821 gaaacctgag gagaaagagt attcacttgt tgacggcgac ggcatgtcct tacttgtaaa
     5881 acctggcggt tcaaagtatt ggcgatttcg tttccgtttt ggcggtaaac aacatttgat
     5941 ggcgttcggc gtttacccgg atgtttcgct ggcggatgcg cggaagaaaa gggaagaggc
     6001 cagaaagctg gtagctgctg gtatcgatcc tcgtgagcat aagcgtgctg tgaaagaaga
     6061 gcaggcgaaa gagattatta ctttcgagaa ggttgccaga gagtggctcg taaccaacca
     6121 aaaatggtcg gaagagcatg ctaatcgtgt aaaaaagagc ctggaggaca atatcttccc
     6181 ggcaattggt tcccacaata ttgctgaact gggcacccgc gatctgttaa ttcctattaa
     6241 agccgtggag aaatccggac gtcttgaagt ggcttcccgc cttcaacagc gcaccacggc
     6301 catcatgcgt tatgcagtgc aaagcgggtt aattgattac aatccggcgc aagagatggc
     6361 gggggcggta gcttccagta atcgacagca tcgtccggcg ctggagttaa agcgtatccc
     6421 ggaattactc cagaaaatcg atgaatacac cggcaggcca ctaacccgct gggcaaccga
     6481 acttaccctg cttattttta ttcgatccag tgagctgcgt tttgctcgct ggtcagaaat
     6541 cgattttgaa acgtcaatgt ggaccatccc gcctgagcga gaacctattc ccggtgtgaa
     6601 acattctcag cgaggagcaa agatgcgtac acctcatctg gtgccgcttt caaagcaggc
     6661 gctagcgatc ctgaagcaga taaaacagtt ctgtggcgaa catgagctga tttttattgg
     6721 cgatcacgat ccgagtaagc ccatgagtga aaacacggtg aacagtgcat tgcgggtgat
     6781 gggctatgac accaaggttg aggtttgcgg ccacggcttc cgcactatgg cctgtagctc
     6841 attgattgag tcagggttat ggtcgaagga tgcggtggaa aggcagatga gccatatgga
     6901 acgcaattcg gtgagggcgg cgtatatcca taaagcggaa catctggatg agcgcaagct
     6961 gatgttgcag tggtgggctg attttctgga tgcgaaccgg gagaagggga ttacgccgtt
     7021 tgactatgcg aagatcaatc ggggtaatgg cgagtgagtt atctaaaagc gcacattcgc
     7081 ctctaaattg aaagctgctg acttattcaa acgaggaatt ggtcagcagt gccaaatagc
     7141 cgagttttca ctaaggtaag cctttttatt gaacctaatc ggcagtaatg ctccacctaa
     7201 gcctaaattt aaagtcatga ttacttataa aaggggagtg gacccttgat tgagccctac
     7261 atcgtaacaa aggtacagca attcggctac atcaggagtg atagtcaatt agttaatcta
     7321 ataattaatt gaatctaaat aattaaattt cttacgtaaa attgaccaat ttactggtgg
     7381 tgtaagccgc aatatcttaa tgtaatcagt ataaaggggc ttgatttctt ttaaagatag
     7441 tatccgtatg aattttaaga aaaaactcga agagcatttt aagcagttcg aagcatcccc
     7501 agtgttattt gtcggctccg gtgtgtctcg tcgttatctt ggtgtgccat gttggcaaga
     7561 ccttttgaag cattttgctg aggcaattgg ggaaaatcat ataaaattaa aaacaaaatc
     7621 taatggtgac ttacccgaat atgcacaatt attggtgtct gcatatgctg agaaatggtg
     7681 ggatactgaa gaaggacaat tagcattaag tgaaaaagaa caggagaaaa ctttcattaa
     7741 cgaacagtct cctttgaagt tgtcaatatc taaatatatt gagaatgcac ataaaaatat
     7801 tatagataat gatgagttaa aacatga
//
LOCUS       DADLTD010000181         7825 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.182, whole genome shotgun sequence.
ACCESSION   DADLTD010000181 DADLTD010000000
VERSION     DADLTD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.182"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(496..858)
                     /locus_tag="JN701_003314"
     CDS             complement(496..858)
                     /locus_tag="JN701_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY7365657.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            1143..1352
                     /locus_tag="JN701_003315"
     CDS             1143..1352
                     /locus_tag="JN701_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365658.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1364..1951)
                     /gene="mtlR"
                     /locus_tag="JN701_003316"
     CDS             complement(1364..1951)
                     /gene="mtlR"
                     /locus_tag="JN701_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY7365659.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1951..3099)
                     /gene="mtlD"
                     /locus_tag="JN701_003317"
     CDS             complement(1951..3099)
                     /gene="mtlD"
                     /locus_tag="JN701_003317"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7365660.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3329..5242)
                     /locus_tag="JN701_003318"
     CDS             complement(3329..5242)
                     /locus_tag="JN701_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY7365661.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5779..6141
                     /locus_tag="JN701_003319"
     CDS             5779..6141
                     /locus_tag="JN701_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7365662.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6144..7280
                     /locus_tag="JN701_003320"
     CDS             6144..7280
                     /locus_tag="JN701_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7365663.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1952 a         1904 c         1901 g         2068 t
ORIGIN      
        1 gtccattgta ttattggtga tttatgttgc tttacattaa tgacagtgat gtgtgtcgat
       61 taaatattgt tttaagcatt catcataaaa atgtatcatt cgtttcaaaa gatgtctgtg
      121 tttgtatgaa tatattattt tcgtggattc taatgatgat tattaataaa tgtcaaatat
      181 gaaagaaacc atgtctggga aaaaatttac agataattta ttgtatgaat atgatgatta
      241 aaatttcaca attaaatccg aaaaaaagag acgtaactgc ttgttaatta ataaatgata
      301 tcttctatat atttttattg tttataggtg gctatttcgg taaaaatgca ttttcagaat
      361 atcaaccagc ggtaaatcga cgtaaaggga gtgaattgtt tggtaattag gcgagtagac
      421 aggagagtcc ccgcattgac cagctaaggc aagccagatg cgggttgata tgatcgaacc
      481 agtcgtctga ggcgcttaaa acgcagtctt agagaatcca gtcatctcct gcaggcccat
      541 ttcacggccc agcgcggtca ttgggtgcac aaccaccagt ccgcgaacac ttttcttcag
      601 cttgcccata tcagcctgct cttttttggt aattgcgcgc tgaaacggca tcttcatcag
      661 cttctgtgct tctttactca gtttttggct atgaacctca cgcaggcgag caatttctgc
      721 ttccagcgtg gctttctctt tttccagctc ggcatatttg tctgcggctt caaccagcga
      781 cagatcggcc tgctggtggc ggatggcgtc caggcgatcg ctgagacgtt tgatttcgtt
      841 tttttcgact tctttcatgt gatggcttct gataaagata aatttatagc ccctagtatg
      901 cgcgaccagg tgctttgttc acaactgatg acattgtaat agttctgcaa cgtaatcatt
      961 aacatgctca aaaactacgc aattcgttga ttaattaaga aatctataga aaaaagataa
     1021 taaacagcag cggatgatta ttgaggattc agcacgtttt ccttaatgtc atctttaccg
     1081 ggaacctaaa ctaaaattgg tggccgcgca ttcccggcgc ggggtaatac ggagatatca
     1141 tcatgggcaa attaggtgaa aacgttccgc ttcttatcga taaagccgta gatttcatgg
     1201 catcaagcca ggcgttccgg gagtatctga aaaaacttcc tccccgtaac gcgattccgt
     1261 ccggaatacc cgatgaaagc gtgccgttat atctacaacg tctggagtat tatcgtcggc
     1321 tttatcggcc gaagcaggta gaggggcagt aagcagacgt tgcttacttt tgaaaggctt
     1381 tttttaagct gattttggaa atcagctcag tcagtgaaag gaccattgtt gatcgcacag
     1441 cctgttgata gcgctgaatt tgcattgcat acaaactgga gtctgctggt tcaaactgtg
     1501 gcggcggtgg taacgccgcc acgcagtgca gttcaccaaa gggtccaagg atttcgtcgt
     1561 cggtaaaggc gtactcgttg ccgtcgtgat ttagctcttc acgcaatgcc atcagcagtt
     1621 ccgcatcttc gtattcctgg cggttaatga cgcccaaccc gtaaatgagt tttaaacgca
     1681 cagaaagatc gcccagcgga ccgtcgccgt cgagtaacgg ttctacagca tacttcaccg
     1741 cgtaatcgtc tttgcggaat acctgaagca ccagaagatt taccgcctcg gtcaaaagct
     1801 cgacggcggt gatcagaaag cttcgcacgg ttttgccagc attcagacgc tcaagcacac
     1861 ggttttcaaa ggcctgggtt tgttccattg ttgcctgcat atctgacaat ctgttattcg
     1921 ggcgcagggt gtcctgcgcc tggtccacca ttattgcatt gctttataag cggttaccgc
     1981 ctcggataca acctcactgt tggcatcaag accggaaatc tgtgccagcg ccgcctgcgg
     2041 acctttgtca gcgatcagtg ctgccagttc ctgagcctgc ggatcatctt cactgcggaa
     2101 gtgcattgca ccagcaatcc cctgaatcag attcttgtgc ggaaggctgt attccagcgt
     2161 accgagcagt ggcttgatca gacggtcgcc agcactcagt ttacgcagcg gctgacggcc
     2221 tacgcgctct acatcatctt tcagatacgg gttctcaaaa cgaccgagga ttttctggat
     2281 gtacgccgca tgtttgtctg cgtcaaagcc gtagcgcttg atcagtaccg caccactttc
     2341 ttccatcgca ccttttacca ccgcgcggat tttctcgtcg agaatagcgt cacgaatggt
     2401 ctgatgaccg gccagttttc cgaggtacgc ggttatagca tgacccgtgt tcagggtgaa
     2461 gagtttacgt tcgacaaatg ccatcaggtt gtcggttaac tccatgcttg ggatgttcgg
     2521 cagtgcgcct ttgaactgcg ttttatcgac aatccattcg ctgaaagttt ctaccgtcac
     2581 ttccagcgga tcgttagttg ccgaagccga aggcggtacg atgcggtcaa cggcggaatc
     2641 gacaaagcca acgtgttctt ctacccacgc tttggcgtct tccggcaggg cgttcatcac
     2701 atggcctttc agctgcgtgg taccgcgtac catgttttca caggcgatga tgttcagcgg
     2761 ggattcatta ccttgttctt tgcgtttcac cagccctttg gcgatagccg gagcaatacg
     2821 ttccagcaca accgggccaa cggcggtagt gactaaatcg acctgagcaa tcagatcaac
     2881 gacatcatca ccaatgctgc tgacagcatt gacgccggaa acggtatcta cctgctcggt
     2941 ttcaccaacc acatgaacct gatagctatg acgggcattc agggcatcaa gtaccacctg
     3001 attgacatcg gcaaacgtca gttggatacc cgcgtctgcc agcagtttac cgataaagcc
     3061 acgaccgata ttacctgcgc caaaatgtaa tgctttcata gtattaacct tcatcaatgt
     3121 ttttacccga gagggctggg gtgaggcttt tccctcaccc aaaccctctc cgtgaggaga
     3181 ggggcagcgc cggatgcggc gtaagcgcct tatccggcct acaagaacgt gcaaattcaa
     3241 caaatcgtga tgcatcctgt aggcctgata agcgtagcgc atcaggcaat taaccccacc
     3301 ttctccatgt ggagagggtg ggattggatt acttacgacc tgccaacagt tccagcactt
     3361 catccacgct ggtggtgtgt gccagacgct cgatgacgga ctcatcatcc agtgcattgg
     3421 tcaggctggt gataacctga atgtgctcgt tgttacgggc tgcaatacca atcaccagac
     3481 gggcaatgtc atcttcctct tcaccgaagc gcacgccttc cgggtactgg cagaacacca
     3541 cgcccgtttt cagtacgcga tctttcgctt caaccgtacc gtgtggcacc gcgatagact
     3601 cacccagata agtcggggtc agtttttcac gatccagcat cgcctgaacg tattccggct
     3661 caacgtaacc gcctttcacc agctgctcgc cagcaaaacg aatcgcttct tctttggttg
     3721 ctgctttgcg accgaggaag atgttctccg cgcccagttt gaacaggtta gcactggaat
     3781 cgtcaaagct gtctttcaga ctgtctttca ctttcacttc gttttcagta tggcgctggg
     3841 cagcaaccag acgttcggtt aggctggtgt acaggccgct gtcgaggaag ttggtcagcg
     3901 aaatatgctg tgcctgcgga acctggcgca tagcgcgttc ggtcaggtca cggtgagtga
     3961 tgacgaggtc cacatctggc ggcaggttgt tgatcgcgct gttagtgacc gaaatctgcg
     4021 acagacctgc atcctgaatt ttcttacgca gcacgcctgc gcccatcgca cttgaaccca
     4081 taccggcatc acaggcaacg atgattttac gtacgtggct caggtcgtta gtcacatcgc
     4141 ccgcagacag cggagatgtg cctttagact cagctttcat gtcctgcata cgacgagttg
     4201 ctgcttcaat atcatcttct tctttcactt tgctggtttt cagcaaaata gcagagacaa
     4261 cgaaggagac agccatcgcc gcacacacac ccgcgatgtt agcgaaataa gccccttttg
     4321 gtgtcatcgc cagtacagca aggatagaac ccggagatgc cggagaaacc agaccaccgc
     4381 ccaggatagt cagcgtgaac acgccagtca taccgccgag gatgactgcg aggatcagac
     4441 gcggattcat cagcacatac gggaagtaga tttcgtggat accacccagg aagtggatga
     4501 ttgccgcacc gcccgcagac tgtttagcgc taccacgacc aaagaacatg tacgccagca
     4561 gcacgcccat acctggacct gggttagctt caatcaggaa gaagattgat ttacccagtt
     4621 catgggactg ctgaataccc agcggcgaga agataccgtg gttaatggcg ttgttgagga
     4681 acaggatttt cgccggttca acaaagatag acgccagcgg cagcatgtca tggacaacca
     4741 tgaagttaac gcccgcagcc agcattttgg acagggcttc aacaatcggg ccaatgccga
     4801 ggaatgccag aatagcgagg atcatcccga tgatgcctgc ggagaagtta ttcaccagca
     4861 tctcaaaacc ggatttgatc ttaccgtcta cccagcggtc gaagtgctta atgcaccagc
     4921 cgcccagcgg acctgcaatc atagaaccga ggaacatcgg catgtctgcg ccgacgataa
     4981 cgcccatggt ggtgatggca ccgactacgc cgccacgttc gccgcctacc agcttaccac
     5041 cggtataacc gatcagcagc ggcaggagat aagtgatcat cggcccgacc agcttcgcca
     5101 gcgtctcgtt cgttaaccac cctgttggaa taaataacgc ggtgatgata ccccacgcga
     5161 taaacgcgcc gatatttggc atcaccatgt tgctgaggaa acgaccaaag ctttgcactt
     5221 tgatcttaat atcggatgac ataaaaacac cccttcttat gtttgctgtc gcgcaggctg
     5281 gaagcccgag gttttttgtt aatgtggcgg cagaggtagc cggaccttgt agatactgcg
     5341 aaatctggca ctgaatcggt taactgtcca gtcgacggcc ttttgtgtga tattaatcac
     5401 ataaatatag gaggtagaga ggtaattgac gtgacgttca tcacaaaacg cttgtgtaaa
     5461 aaagcaacaa ggcggcgatt ttcgctaaaa atggcattta aatgtgagta gtgtcacatt
     5521 tttgttttga tggtttgttg aatctttgtg atctgaatca caagatattt tcttacggcg
     5581 ttcacctgat gtgatctaca gcatgttatg ccttcgtcat acaatcgtta tgtaactaag
     5641 caactcatca cctctaaaaa ttattctttg gtgttatggc gagaatcgcg aacccggatt
     5701 attgtcaaaa tatcagcctg actcccttct ggctgccagc ccaaaaacaa cactgataaa
     5761 cgtgaatccg aggcagatat gtttctaaac tatttcgcgt tgggagtgct gatcttcgtc
     5821 tttctggtga ttttttatgg aatcatcgcg atacatgaca tcccttatct gattgccaaa
     5881 aagcgcaatc acccccatgc cgacgctatt catacggcgg gctgggtgag cctgtttact
     5941 ctgcatgtta tctggccgtt tctgtggatc tgggcgacgc tctatcaacc ggagcgtggc
     6001 tggggtatgc agtcacatgt tgcgtcgcag gagaaagcga ctgacccgga aatcgcctca
     6061 ctttctgacc gaatttcccg gctggagcat caactcgccg ccgagaaaaa gactgactat
     6121 tccacgttcc cggagatcta attatggatt tattgattgt tttaacttac gtggcgctgg
     6181 cgtgggcggt ctttaaaatc ttccgcattc cggtaaatca gtggacgctg gcgacggcgg
     6241 cgctgggagg cgtgtttctg gtgagtggtt tgattttgtt gatgaactac aaccaccctt
     6301 acacttttac cgcgcaaaag gcagtgatag cgatccctat cacgccacag gtgacgggaa
     6361 ttgttactga agtcactgac aagaataatc agcttattca aaagggcgag gtgcttttta
     6421 agctcgaccc ggttcgttac caggcgcgag ttgacagact tcaggctgac ctgatgacgg
     6481 cgacgcataa tataaagacg ctgcgtgcgc agctcactga agcgcaggcc aacaccaccc
     6541 aggtttcagc ggagcgcgac cgtctgttta aaaattatca acgttacttg aaaggcagcc
     6601 aggcggcggt gaatccgttc tcggaacgtg acatcgacga tgcgcggcaa aatttcctcg
     6661 cgcaggatgc gctggtgaaa ggctcggtgg cggagcaggc gcagatccag agccagctcg
     6721 acagtatggt taacggcgag caatcgcaga ttgtgagctt aagagcgcaa cttactgaag
     6781 caaaatataa ccttgagcag actgtcattc gcgcgccgag caatggctac gttactcagg
     6841 tactgatccg cccaggtaca tacgcagctg ccttgccgct gcgtccggtg atggtcttca
     6901 tccccgagca aaaacggcaa attgtcgccc aatttcggca aaactcgctg ttacgtctga
     6961 aacccggcga tgatgcggaa gtggtgttta acgcgctacc tgggcaggtg tttcacggca
     7021 aactgactag tattttacct gtcgtgccag gcggttctta tcaggcgcag gggggattgc
     7081 aatcattaac ggtcgtgccc ggcacggacg gtgtgcaggg aaccattgaa ctggacccta
     7141 acgatgatat cgatgcctta cccgacggca tctacgccca ggtggcggtt tactccgacc
     7201 atttcagcca tgtttcggtg atgcggaaag tgctgctaag aatgaccagc tggatgcatt
     7261 atctttattt ggatcattga gggggagggg ggagattatg gaactttgct ccttgtaagg
     7321 actaattatt tttgcatatt acttcattag ctgcgatgat ttttctttaa tattcattat
     7381 gttaataagt tctggatgag tgtgctctca ttggattttt tatccgtctt gttctgccgc
     7441 aggatgagac tatacatcgt cgagatcgca atgatgatat gagttgttcc tcctgcgacg
     7501 gctctaatca aaaaagagca ggagtgcttt tccatctatt gcgatgatgg ctttattatc
     7561 tgaaggatga cagtcacatt taaaaaatat ttaatctata gatcacaatg tagggtcatg
     7621 gaatatatgt atacaactat tttcttcttc acttttaatt atcgtattca ttataaaact
     7681 aagttaaaaa aactcctgga gagtgagtta ttaatatatt tttaaataaa attaagttat
     7741 cattcatcga tgcattttat agaatcatca atggccataa tagtactatt aactctcaat
     7801 tttatatttt tatcatcact acaaa
//
LOCUS       DADLTD010000182         7818 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLTD010000182 DADLTD010000000
VERSION     DADLTD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.183"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            268..411
                     /locus_tag="JN701_003321"
     CDS             268..411
                     /locus_tag="JN701_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY7365664.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            424..633
                     /gene="cspE"
                     /locus_tag="JN701_003322"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             424..633
                     /gene="cspE"
                     /locus_tag="JN701_003322"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7365665.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            799..1608
                     /gene="rlmA"
                     /locus_tag="JN701_003323"
     CDS             799..1608
                     /gene="rlmA"
                     /locus_tag="JN701_003323"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY7365666.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="JN701_003324"
     CDS             complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="JN701_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY7365667.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2599..3057)
                     /locus_tag="JN701_003325"
     CDS             complement(2599..3057)
                     /locus_tag="JN701_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY7365668.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3112..3963)
                     /locus_tag="JN701_003326"
     CDS             complement(3112..3963)
                     /locus_tag="JN701_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7365669.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3976..4776)
                     /locus_tag="JN701_003327"
     CDS             complement(3976..4776)
                     /locus_tag="JN701_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7365670.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4839..5810)
                     /gene="manX"
                     /locus_tag="JN701_003328"
     CDS             complement(4839..5810)
                     /gene="manX"
                     /locus_tag="JN701_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY7365671.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6273..>7818
                     /gene="yoaE"
                     /locus_tag="JN701_003329"
     CDS             6273..>7818
                     /gene="yoaE"
                     /locus_tag="JN701_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY7365672.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1985 a         1913 c         1941 g         1979 t
ORIGIN      
        1 gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga actatgttag
       61 aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
      121 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
      181 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt
      241 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
      301 aaacacgttg atgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
      361 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
      421 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
      481 attactccgg ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat
      541 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
      601 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
      661 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
      721 cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag
      781 tatcctgccg gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt
      841 gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat
      901 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
      961 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
     1021 gtcgcccaac tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt
     1081 ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt
     1141 ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt
     1201 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
     1261 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
     1321 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
     1381 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg
     1441 gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
     1501 ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
     1561 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
     1621 gtccagagga tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt
     1681 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag
     1741 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca
     1801 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
     1861 ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
     1921 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca
     1981 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
     2041 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct
     2101 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
     2161 gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
     2221 aacatgcata catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt
     2281 gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa
     2341 cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc
     2401 gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat ttcattgctt
     2461 tattgataac gaattcattt agatttatat atcaatgaaa cgttaaaata aataacaaat
     2521 aagggaagag gtaatatagc ctaagctatg tctgattatt aaaaatacac taaacatagc
     2581 tttggctaaa ttcataactt attgagttaa aacgataagc ttataaattc tttccagatt
     2641 gtcgatattt cgaacgcgga ttaacagccg acgctgttct aattgcatca ccaacacgcc
     2701 atcttccgac aagttcatcg ctttgattcg gctatattca atccagacat tggcgaagaa
     2761 aaaacctttt tgtttaaaga tgatcttcgg aacgcggatc cagaatatat aaaaacccat
     2821 cagagccagt gcgcttaata accatgtcgt tattaatgca ccatgattcg tgacgttgtt
     2881 atagataaga atgacaatca atccgacgaa gataacgcta tcgatgcgac cacgccggag
     2941 caaaggaatt gccagcaggg tggggccgtt acggcggggc atgatgaact gatcgtagat
     3001 cgcgaaggcc aggagtgcgg cgatgaaaag aatcagtacc aggtccgtga ttgtcattaa
     3061 tcctccagat aaaaaaacgg ggccaaaagg ccccggtagt gtacaacagt cttacagtcc
     3121 cagcaggccg caagcgtaac cagcgatacc gatgacgaag aagccaacga tgatccacag
     3181 cgggttaact tttttgcgca gtagccacat acaagcaaag gtcagcagca gtggtaccag
     3241 gcctggcatt aactggtcca gaatagtctg gacagtagta acgtgttctt tgcccgtctg
     3301 gtcagtaatg cgagagacaa ccagcgggat gttgacatgt gtccacttgt taaccaatgc
     3361 ccccatgaca aacaggccga ggatagacgc cccttccgtc agtttttgca ggaagccacc
     3421 acccatatct ttaacgatat cgataccttt ggagtaacca tacgctacgc cgtagtaacg
     3481 ggttgccaga cgcaccaggt taaacaggat gaagaacagc agcggaccta acaggctgcc
     3541 gctcatcgcg ataccggcac ccagtgctgc aaataccgga cgtacggttc cccagaagat
     3601 cgggtcgcct acaccagcca gcggccccat caaaccgact ttgataccgt tgatagcacc
     3661 gtcgtcgatc tctgcgccat tagcacgctg ttcttccagc gccagggtta cgccgagaat
     3721 cggcgcagcc acgaacggct gggtgttaaa gaactccagg tgacggcgaa tagcttgttt
     3781 acgagcttcg ttgttctcag ggtagaggcg acgaattgcc ggtaccatag agaagcagaa
     3841 acccagtgcc tgcatacgtt cgaagttcca tgaaccctgg aagaggttag aacgcaggaa
     3901 gacgccacga atatcacttt gagtgagttt tttctcggtg gtagtttgag ttgtatcaac
     3961 catttcgctc acctgttagt ccagttcgtt atcgagatcg ttgttaccag cagcctgagc
     4021 aggcgcaccg gctacgcggt tgtatttcgg gctaagttgg atgtagagca ctgccataac
     4081 agtaccgatc acacccagag caaccaggtt aaagttggtg aatgctgcgg ttacgaagcc
     4141 gaggtagaag aacggcatca ggtagccagc acgcatcatg ttgataacca tcgcataacc
     4201 aaccaccacg atcatgccac cggcgatatt cagaccattg gtcaccactt ccggaatcgc
     4261 attcagcatg ttctgtactt cgctggtacc aacagacagc gcaacgatga cggccggaat
     4321 agccacacgc attgcttgca ggaacagaga agaaacgtgg atccaggaaa tcgctgtcag
     4381 gttgccgtta tcagcagcct tatccgcagc gtgctggaaa gcaacggtaa tagtacgaac
     4441 gatgatggtc agtacctggc ccgcagcggc cagagggatt gccagtgcga tacctgcacc
     4501 aatgctctga tgacctgcga taaccagaat ggtagaaatg atagaagcca gagcggcgtc
     4561 aggcgcaact gcagcaccga tgttcatcca gcccagcgcg atcatttcca gcgtaccacc
     4621 gataataata ccggttttca tatccccaag aacgataccc accagggtac acgcgattag
     4681 cggacggtga aactgaaatt catcgaggat tgatcccata cctgcgatac aggctacgat
     4741 aaataccagc acaatttgaa gagtggtaat ctccattgta cttctcctgt ttacgtaaga
     4801 cttaagtgtg aaaactgagt gataatcaac acaatacgtt acttatcgat tttgctgatc
     4861 agatccatca ttttcagttt cggatcggtg gaaaccttac ggacttccag ctcaataccg
     4921 cgcgcattca gtttcttgaa cgcctcgata tctttttcat caaccgaaac cgcgttattc
     4981 acctgggttt taccctgacg gaatgccata ccaccgacgt taacagaggt gattttcacg
     5041 ccgccttcaa cgagacgctc tacatctgtt gggttggtaa ataacagcat tacgcgttcg
     5101 ccagcatatt tcgggttgtt gtagacgcga atcattttgg caacatcaac tacgtgtgct
     5161 gttacgcccg gaggtgcaac ctgggtgagc agtgtcttac gaacggtatc cgcagccact
     5221 tcatcactaa caacaataat acgggagaca ttggtttctt tggtccagcg ggtggcgacc
     5281 tgaccgtgaa tcagacggtc gtcgatacgc gcaaggccaa taaccatgta gtcgtttggc
     5341 cccattggtt ttgccggagt tggagccgct tttggtgctg cggcagcggg tgctggcgcg
     5401 gctttttcaa ccggtttggc tttcagtgct ttcacgcctt cacggcctgt ttctactgcc
     5461 agcgcaacca gttcatcaaa gcttgggtca tcatcacgag ccattaacgt ttccacgagc
     5521 attggaatgt taacgcctgc aatgacttca taatgctctt tgtcgacgac aatgcggctg
     5581 gcagcattga acgggctgcc tccccatgta tcaacgagaa acagcacgcc tttagtggtg
     5641 tcgagttttg ccaactgagc gttgtacttt tcaatcagcg tttcggcatt ttcacctgga
     5701 acgaaatcga tccagccgac gttttcctgc tcgcctaaca gcatttctgc cgttttaagc
     5761 aactgctctg cagcccaacc atgtgtgcct ataacaatag caatggtcac ttgctacctc
     5821 ctttattatc gttaacacct caacgtgcca gatgtatttt tgaatcgtcg tccacaatcg
     5881 aatcgattca gataagggct acaaccaaaa acccctgcgt ttcgcgattt attttagata
     5941 tcgaaaaaat tattttatgt gatgaagatc cgtaatttaa ctttcgatta gcagaaattt
     6001 cgaaaggtaa aatatccttg tcacattcgt ttgcaaagga aggtaaatct ttgccaaatc
     6061 agaggcgtct ctgatatgtt taactcccgt ttaataacca tggagtatag ggcagtagcc
     6121 cgcagtatgg atcgtcaccg acgtcatttc agcatcaggc cttttaacgc ctgcctttct
     6181 ggctctctat gccgcacctt tcgtttgcat tttgtcgtta cgcctgcatt atttctggcg
     6241 tcgaatagct attccttaag caggagcttg tcatggaatt cttaatggac ccctcaattt
     6301 gggcggggct actcacgctt gttgttctcg aaattgtgct gggtatcgat aacctggtct
     6361 tcatcgccat tcttgctgac aaactgccgc caaaacaacg cgataaagcg cgtttgctgg
     6421 ggttatcact ggcgctgatt atgcgtctgg ggctgctgtc gctgatttca tggatggtca
     6481 cgctgaccaa accgctattt accgtcatgg atttctcctt ctccggacgc gacctgatta
     6541 tgttgttcgg ggggatattc ttgctgttca aagcaacaac cgaactgcat gaacggctgg
     6601 aaaaccgcga tcatgattcc ggccacggta aaggctacgc cagtttctgg gtggtcgtca
     6661 cacagattgt catccttgac gccgtcttct cgttggatgc ggtaattact gcagtaggga
     6721 tggttaacca tctgccggtg atgatggcgg cggtagtgat tgcgatggcg gttatgttgc
     6781 tggcatccaa accgctgacg cgattcgtta accagcaccc cacggtggtg gtgctctgtc
     6841 tgagcttcct gttaatgatt ggtcttagtc tggtggcaga aggtttcggt ttccacattc
     6901 cgaaaggtta cctgtatgcc gcgattggct tctcgatcat catcgaagtg tttaaccaga
     6961 ttgcgcgtcg caactttatt cgccaccagt cgactttgcc gctgcgagcg cgtactgccg
     7021 atgccatcct gcgtttgatg ggcgggaaac gtcaggccaa tgttcagcac gatgccgata
     7081 acccgatgcc gatcccggaa ggtgcatttg ccgaagaaga acgttacatg attaacggcg
     7141 tactgacgct ggcgtcgcgt tctctgcgcg ggatcatgac gccgcgcggt gaaataagct
     7201 gggttgacgc taatctcggg gtcgatgaaa tccgcgagca actgctctct tcaccgcaca
     7261 gtctgttccc ggtatgtcgc ggtgaactgg atgaaatcat cggtattgta cgtgctaaag
     7321 aactgctggt ggcgctggaa gagggcgttg atgtggcggc gattgcttcg gcgtctccgg
     7381 cgattatcgt cccggaaacc ctcgatccga tcaacctgtt gggcgtgctg cgtcgtgctc
     7441 gcgggagctt tgttatcgtg accaacgagt ttggtgtggt acaaggtctg gtcacgccgc
     7501 tggatgtgct ggaagccatt gcgggtgaat tcccggacgc tgacgaaacg ccggaaatca
     7561 ttactgatgg tgacggctgg ctggtaaaag gcggtacaga tttgcatgcc ttgcagcagg
     7621 cgcttgatgt tgagcacctt gccgatgacg atgatatcgc gacggtcgcg ggcctcgtga
     7681 tctcggcaaa tggtcacatt ccccgtgtgg gcgatgtgat tgatgtaggg ccactgcata
     7741 tcaccatcat tgaagccaat gattatcgtg ttgatctggt tcgcattgtt aaagagcaac
     7801 cggcgcacga tgaagatg
//
LOCUS       DADLTD010000183         7645 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLTD010000183 DADLTD010000000
VERSION     DADLTD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.184"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(174..704)
                     /gene="ppa"
                     /locus_tag="JN701_003330"
     CDS             complement(174..704)
                     /gene="ppa"
                     /locus_tag="JN701_003330"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY7365673.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1014..1970
                     /gene="ytfQ"
                     /locus_tag="JN701_003331"
     CDS             1014..1970
                     /gene="ytfQ"
                     /locus_tag="JN701_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAY7365674.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2110..2646
                     /locus_tag="JN701_003332"
     CDS             2110..2646
                     /locus_tag="JN701_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7365675.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQVISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDT"
     gene            2650..3612
                     /locus_tag="JN701_003333"
     CDS             2650..3612
                     /locus_tag="JN701_003333"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7365676.1"
                     /translation="MELLFGLMRQLRDRGVSLIFVTHFLDQSYQVSDRITVLRNGSFV
                     GCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTIAPFDLE
                     VRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASVLGIGFC
                     PEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIRTPSTEQ
                     PIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCADGLALL
                     VISSELEELVGYADRVIIMRDRKQVAEIRLAELSVPAIMNAIAA"
     gene            3626..4648
                     /locus_tag="JN701_003334"
     CDS             3626..4648
                     /locus_tag="JN701_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7365677.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4635..5630
                     /gene="yjfF"
                     /locus_tag="JN701_003335"
     CDS             4635..5630
                     /gene="yjfF"
                     /locus_tag="JN701_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY7365678.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDTIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="JN701_003336"
     CDS             complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="JN701_003336"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY7365679.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6837..>7645
                     /gene="mpl"
                     /locus_tag="JN701_003337"
     CDS             6837..>7645
                     /gene="mpl"
                     /locus_tag="JN701_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY7365680.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVG"
BASE COUNT         1756 a         1927 c         2101 g         1861 t
ORIGIN      
        1 tccagcaagt ggctatgaac agcaaaatgg tggtcgggtt tgcttgttct taccgttcaa
       61 gccttgcaac cgctgagaat aatgatggtg gaatagagag tttatttatc gcgggcataa
      121 aaaaaccctt actaaccgaa gcccggcgtt cagggttatt gcgctagaag aacttattta
      181 ttctttgcgc gctcgaagga agcaacgatt tcagctttag cggcttctgc gttttcccaa
      241 ccttcaactt tcacccactt gcctttttcg aggtctttgt agtgctcgaa gaagtgagcg
      301 atttgcgctt tcagcagttc aggcagatcg tttacgtctt taatgtgatc gtattctttg
      361 ctcagcttgc tgtgcggaac cgcaaccagt ttcgcatctt caccggcttc gtcggtcatt
      421 ttcagaacgc caaccggacg gcaacggatc acagaaccag gctgcagcgg gtacggagtc
      481 gggaccagta cgtcaaccgg gtcaccgtcc agagacaggg tgtggttgat gtaaccgtag
      541 ttgcacggat agaacatcgc ggtggacatg aagcggtcaa cgaacagtgc gccgctctct
      601 ttgtcgattt cgtatttgat cggatctgcg ttagccggga tctcaataac aacgtagatg
      661 tcttccggca gatctttacc cgcagggacg ttgagtaagc tcatgtctgt ttcctttaaa
      721 aatatgtggc aaacaagtgc cgagtattat agccaactcg cgccgaatgt ctttgcttgt
      781 tttcgtcttc attttccttt ttcgccagtt ttaagacggt atacagagca ggaaaatcca
      841 taaccgtagc cgcatttttc atagtgagat gaaagcgatt acaaacttgt gattaacgtt
      901 ttatttactt ttttgaagtg tgatgtaacg caatccgtta cataacgaat tgtctatagt
      961 tttttcgcga acatcttttc accaataata attaccccga cgaggacaac cctatgtgga
     1021 aacgcttact tgtagtctct gcagtctcgg cagccatgtc gtctatggcg ttggccgctc
     1081 cattaaccgt aggattttcg caggtcggat cggaatccgg ctggcgcgcc gcagaaacca
     1141 atgtggcgaa aagtgaggcc gaaaaacgcg gaattacgtt gaaaattgcc gatggtcagc
     1201 aaaagcagga aaaccggatt aaagcggtac gttccttcgt tgcacaaggg gtggatgcga
     1261 tctttatcgc tccagtggtt gcgacgggtt gggaaccggt attaaaagag gcgaaagatg
     1321 ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt gaaagacaaa tctctctata
     1381 tgaccaccgt tactgccgac aacatcctcg aaggtaagtt gatcggtgac tggctggtaa
     1441 aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca gggcaccgtt ggggccagcg
     1501 tcgccattga ccgtaagaaa ggctttgccg aagccattaa gaatgcgcca aatatcaaaa
     1561 ttatccgctc gcagtcaggt gacttcaccc gcagtaaagg caaagaagtc atggagagct
     1621 ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt ttacgcccat aacgacgaca
     1681 tggtgattgg tgcaattcag gcaattaaag aagcgggcct gaaaccgggc aaagatattc
     1741 tgacaggttc tatcgatggc gtaccggata tctacaaagc gatgattgat ggcgaagcga
     1801 acgccagtgt tgaactgacg ccgaatatgg cgggacccgc ttttgacgcg ctggagaaat
     1861 acaaaaaaga cggcaccatg cctgaaaagc tgacgttaac caaatccacc ctttacctgc
     1921 ctgataccgc aaaagaagag ttagagaaga agaaaaatat ggggtattga gggttgctat
     1981 gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc gcatccggcg atggtgcact
     2041 gaagcctgat gcgacgctta ccgcgtctta tcatgcctac tgggagcacg ctttacaccg
     2101 ggggaaacca tgacgaccga ccaacaccag gaaatcctcc gcaccgaagg attaagtaaa
     2161 tttttccccg gcgtcaaagc gttagacaac gttgatttca gcctgcgccg tggcgaaatc
     2221 atggcgctgc tcggtgaaaa cggggcggga aaatcaacgc taatcaaagc attaactggt
     2281 gtataccacg ccgatcgcgg caccatctgg ctggaaggcc aggttatctc accgaaaaat
     2341 accgcccacg cgcaacaact cggcattggc accgtctatc aggaagtcaa cctgctaccc
     2401 aatatgtcgg tcgctgataa tctatttata ggccgcgaac ccaaacgctt cggccttcta
     2461 cgccgcaaag agatggaaaa gcgcgccacc gaactgatgg catcttacgg tttctccctc
     2521 gacgtgcgcg aaccgctcaa ccgcttttca gtcgcgatgc agcaaatcgt cgccatttgc
     2581 cgggctatcg atctctccgc caaagtgctg atcctcgatg aacccaccgc cagcctcgac
     2641 acctaggaag tggagctatt atttggcctg atgcgtcagt tgcgcgatcg cggcgtcagc
     2701 ctgatctttg tcactcactt tctcgatcag agctatcagg tcagcgatcg gatcaccgtt
     2761 ttacgcaacg gcagtttcgt aggctgtcgg gaaacgcgcg agctaccgca gatcgaactg
     2821 gtaaaaatga tgctggggcg cgagctggat acccacgcgc tacagcgtgc cgggcgaaca
     2881 ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg gcaaaaaagg aacgatagca
     2941 ccgtttgatc tcgaagtgcg ccccggcgag atcgtcggcc tggcaggctt gctgggatcc
     3001 ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac ctgctgacag cggcacggcg
     3061 ttgatcaaag gcaaaccgca aaacctgcga tcgccacatc aggcttcggt actgggcatc
     3121 ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg ctgccgcctc ggtgcgggaa
     3181 aatatcatcc tcgctctcca ggcccagcgc ggctggctac gacccatttc ccgcaaagag
     3241 cagcaagaga ttgccgaacg ctttatccgc cagcttggca ttcgcacacc ttcaactgaa
     3301 caaccgattg aatttctctc cggcggcaat cagcaaaaag tgttgctttc acgttggcta
     3361 ctgacccgac cgcaatttct gatcctcgat gagccaaccc gtggcattga tgttggtgcc
     3421 cacgccgaga tcatccgcct gattgaaacg ctatgcgccg atggtctggc gctgctggtg
     3481 atctcctccg aactggaaga actggtgggc tatgccgacc gggtgatcat catgcgcgat
     3541 cgcaaacagg tggcggagat ccggctggca gagctttccg ttccggctat catgaacgcc
     3601 attgcggcgt aaggagaaca gtgtgatgcc tcaatctctt ccggacacca ctacgccaaa
     3661 aaggcgcttt cgctggccga cggggatgcc gcagctggta gcactattgc tggtgctgct
     3721 ggtcgacagc ctggtagccc cgcatttctg gcaggtagtg cttcaggatg ggcgtttgtt
     3781 cggtagcccc atagacattc ttaaccgtgc agcccccgtt gcgttactgg cgattggtat
     3841 gacgctggtg atcgccaccg gtgggattga tctctccgta ggggcggtga tggctatcgc
     3901 cggagccaca acggctgcga tgacggtcgc ggaagtcagc ctgccgattg ttttgttaag
     3961 cgccctaggc accggcatcc tggcgggatt gtggaacggc atactggtag cgatcctcaa
     4021 aattcagcca tttgtcgcca ccctgatcct gatggtcgcc gggcgcggcg tggcgcaact
     4081 gatcacctcc ggacagatcg tcacgtttaa ctcgccggat ctctcatggt ttggcagtgg
     4141 atcgctgttg ttcctgccaa cgccagtcat tattgcggta ctgacgcttc tcctgttctg
     4201 gctgttgacc cgcaaaacgg cactggggat gtttatcgaa gccgttggta tcaacattcg
     4261 ggcggcaaaa aatgccggag taaacacgcg gatcatcgtc atgctcactt atgtgttgag
     4321 cgggctgtgt gcggcgattg cgggcattat cgtggcggcg gatattcgcg gtgccgatgc
     4381 caacaacgcc ggattatggc tggagctgga cgccattctc gcggtggtga ttggcggcgg
     4441 atcgctgatg ggcgggcgct ttaacctgct gctctcggta gtgggggcgc tgattattca
     4501 ggggatgaac accggaattt tgctttcggg ctttccaccg gagatgaacc aggtggtgaa
     4561 agcggtggtg gtgctttgcg tgctgattgt ccagtcgcaa cgctttatca gtctgattaa
     4621 aggagtacgt agccgtgata aaacgtaatt tgccactgat gatcaccatc ggcgtctttg
     4681 tgctgggcta tttctactgc ctgacgcagt ttcccggttt tgcttccaca agagtgatct
     4741 gcaatatcct gaccgataac gcttttctgg ggattattgc cgttggtatg acctttgtga
     4801 tcctctctgg cggtatcgat ctctccgtcg gttcggtgat cgcctttact ggcgtgtttc
     4861 tggcaaaagt ggttggcgat ttcggcctct cgccgctgct ggcgtttccg ctagtgctgg
     4921 tgatgggctg tgccttcggc gcatttatgg ggcttctgat cgacgccctg aagatcccgg
     4981 catttatcat tacgcttgcg gggatgttct ttttgcgcgg cgtcagctat ctcgtttcgg
     5041 aagagtcgat tccgataaac catcccattt atgacacgct ctcaagcctt gcgtggaaaa
     5101 tccccggcgg cggtcgcctt agtgcgatgg ggctgttgat gctggcggtg gtggttatcg
     5161 gtattttcct tgcgcatcgt acccgttttg gcaatcaggt gtacgctatt ggcggcaacg
     5221 cgacgtcggc gaacctgatg gggatttcta ctcgcagcac tactattcgc atttatatgc
     5281 tctccaccgg actggcaaca ctggcgggga ttgtcttctc gatttatacc caggccggat
     5341 atgcactggc gggcgtaggt gtggaactgg acaccatcgc gtcggtggtg attggcggta
     5401 cgttgttaag tggtggcgtc gggacggtgc ttgggacgct ttttggcgtg gcaattcagg
     5461 gactgatcca gacttacatc aactttgacg gcacgctgag ttcctggtgg acgaaaatcg
     5521 ccatcggtat tttgttgttt atttttatag cattacagcg tgggttaacg gtgctgtggg
     5581 agaatcgtca gagttcgcca gtgacaagag tcaacattgc gcagcgataa aaatgcctct
     5641 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
     5701 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
     5761 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
     5821 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
     5881 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
     5941 cgatataacg tgaggtatac gggcggttgg tggatttatc ttcttcctgg cagaatttaa
     6001 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
     6061 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
     6121 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
     6181 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
     6241 ccggcgtgcc gacgggcgta actcggcggt agatggagaa aatggtaccg acagagacgt
     6301 taacatcgat gttggacgag ccatccagtg ggtccatcag caccacgtat tttgcatgtt
     6361 cacagccttc aaagacaaca atctcatctt cttcttcaga ggcaatgcct gcaacgatat
     6421 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
     6481 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
     6541 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
     6601 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     6661 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     6721 tcaatgttgc gctcaggtga atcgcgccag caaattacgg attatcctga aatgcgtttc
     6781 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     6841 gcattcatat tttaggaatt tgtggcacgt ttatgggcgg tctggcgatg ctggcgcgcc
     6901 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     6961 ttgagaagca aggcattgaa ctgattcagg gttacgatgc cagccagctc gatccgcagc
     7021 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     7081 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     7141 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     7201 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     7261 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     7321 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     7381 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     7441 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     7501 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     7561 gtgagcaggg gcactggcag gcgaaaaagc tgaccactga tgcttccgaa tgggaagtct
     7621 tgctggatgg cgaaaaagtg ggcga
//
LOCUS       DADLTD010000184         7640 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLTD010000184 DADLTD010000000
VERSION     DADLTD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.185"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            565..3978
                     /locus_tag="JN701_003338"
     CDS             565..3978
                     /locus_tag="JN701_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7365681.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTAVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4042..5676
                     /locus_tag="JN701_003339"
     CDS             4042..5676
                     /locus_tag="JN701_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY7365682.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5685..6755
                     /locus_tag="JN701_003340"
     CDS             5685..6755
                     /locus_tag="JN701_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY7365683.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6880..7596)
                     /locus_tag="JN701_003341"
     CDS             complement(6880..7596)
                     /locus_tag="JN701_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365684.1"
                     /translation="MLSETKKRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEE
                     GVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVFENGYYDWGCVVNDVLP
                     NICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLNITPISFQSQGWDKRRS
                     ETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDEAMNTQVL
                     SSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2221 a         1741 c         1791 g         1887 t
ORIGIN      
        1 gtcctgttct cccaaatatc atggtattga tacgggagat tatgccgtac aggtacgaca
       61 cgttatgtcg tgccaaaggg gagtggatag aaaaaatggc gaagatgcga aaagttgatt
      121 aatgcattga aaaaaatgaa ttaataatga acttgggtaa aagcgccatc aaaatacgac
      181 acgacctgtc gtattttgtg ttagccggga aaagatatct gctacttact aaaatgattt
      241 agtgctgaaa atatttataa gccaataaat agacaggtat tacggagtaa cgcccaggct
      301 attttaacga gcctgctgtt ttattggctc aaaaaacata tcccatagat gatcagccat
      361 acccgcgtta gtgagcaagg tatcccttga gtagccaggg ctgaagacgc ccattgatgg
      421 gcgaaaacca aggttctggc cgttaaagcg gctatgtcgt taaacacatc accaccatat
      481 gccatccggc tcacttcgtc ggccaaatta gtgtgatatt ctttccagaa tttctgttgt
      541 atccacaaca taaggcgatg attgatggag cagtctctca attttgaaat gttgcgcagc
      601 cagtggccgg aactggcaga actcgcatgt atggcagagc gctatgttca ctccgatccg
      661 gaaagctgcc tggttaagct gcgcaactac accgaattga tggtgcgctg gttgtatcgt
      721 caggagcggt tgccggaagg tattaaggct aatctttacg atttaatgaa cgctgatgtc
      781 tttaccagca tgatgccgga agccatcatc atgaaaatgg atgcgctgcg tatccatggc
      841 aaccgtgccg cgcacggcgg acgtatcaaa gctaaagata cttactggct gctcaaagaa
      901 gcgtatttgt tgggaatttg gctgtatgtt cgctacgccc acggtaatgt tgatgactgc
      961 cccaaattta cactccctcc attaacacaa tcttcaggtc gtgcagatga aaaacgtctg
     1021 gaagatgcaa tcaaggctca ggatgaaagc cgtgagcgcg aactggcgct acaacgcgca
     1081 ctacagcaag aacaggaaaa ggccgaacac ctcactcaac gcctgaatga agccagagcg
     1141 cgtaaccagc atgttgccga tattctctct attgatgaag cggaaacccg ccgtcgcctg
     1201 attgactctc gcttacttgc agccgactgg aatgtagggg aagaacttaa aaacacggat
     1261 caagtcacac aagaacatcc agtcaaagag caacccacca cctctggtga cggttatgca
     1321 gactatgtct tgtgggatga ggcacacaaa ccgttggcgg tggtggaagc aaaaaaaacc
     1381 agcgtcaatg ccgagcaagg acgaattcag gcccggctgt atgcagactg gctggaaaaa
     1441 gaatacggtc agcgtccggt catcttctac accaacggct atgatatctg gctgtgggat
     1501 gaccataaaa ctcatggtta tcccccgcgt cgggtgtttg gcttctacag caaggaaagc
     1561 ctgcaatatc ttattcagca gcgtgaaacc cgtctgccgc tgaacagagt gccacacgta
     1621 aaagataacg aaggtaaggc cgttgccgga cgtttgtacc agctcgaaac cattgcccgc
     1681 gtcagtgaac ggtttaccaa caagtaccga caatcgttaa ttgttcaggc tacgggtaca
     1741 ggcaaaaccc gcgtggcaat tgcactaagc aaactgatga ttgatgcccg ctgggtaaaa
     1801 cgcgtcctgt ttctttgcga ccgtaaagaa ctgcgtaaac aggcggcaaa tgcctttaat
     1861 caattcacca atgagccgct gtatgtggtc gggaaatcga aaaaagcaga caggcagaat
     1921 gccagaatct acatcgctac ctatcccggt atgatgaaaa tcatggacca tttcgatgtc
     1981 ggttatttcg atttgatcat cgccgatgaa tcccaccgct ctatctacaa cgtctacggc
     2041 gatctgttta agtattttga tgccctccag attggcctga cggccacgcc aattgacatg
     2101 gtgagtaaaa ccactttcgg cctgtttggt tgtgaaggac gtattcctac cgccaactac
     2161 agcctggaag atgccatcgc cgataacaat ctggttccct atgaggttgt cacacacaca
     2221 acagaattcc tgcgcgaagg tatcaaacgg gagaaattaa gcgacgcaca aatccgcgag
     2281 ctggaagaac aaggcataga tcccaatacg ctggagtttg atggcaaggc gctggatgag
     2341 gcgatctaca acaaagacac caaccgctat atcctgcgta acctgatgga aaatggcctg
     2401 aaagatcggg acggccagtt gcctggtaaa accatcattt tcgcccgtaa ccacaaacac
     2461 gcgctgctgt tgaatgaatt gttcgacgat atgtacccac agtttgccgg gcgcttctgc
     2521 caggtcatcg acaactacga tccccgcgcc gagcaactga ttgatgattt taaagggctg
     2581 gatgaaagca ccaacaaaga gctgaccatc gctatctccg ttgacatgct cgataccggt
     2641 attgatgtcc cggaaattgt gaatctggtc tttgccaaac cggtcaaatc aaaagtgaag
     2701 ttctggcaga tgattggccg tggtacgcgc ctctgtccgg ggttgtacgg ttacgacgat
     2761 aacggcaaac cgctggataa acagaaattc cgcattttcg atcactgggg caattttgag
     2821 tatcacgagc tgcataccga agaggcggaa gtcacagcga caaaatcgct tgcgcaaaaa
     2881 cgcttcgaag cgtgggttat gctgggggcc gctgcgcagc gtaaattcga caaacaggcg
     2941 gtggatttag ttgctcacca gcttcgcgag caaatcaacg cactggatga aaagtcgatt
     3001 gccgtacagg aaaaatggca gcagaaagcg cagtacagtg atgaaaaggt gctgcgccag
     3061 ctttctccga aaacacagca ggatctgcta tctgttctgg ccccgttgat gcagtggctg
     3121 gacgtgcgcg ggcaaagcga tgccatacgc tttgatatgg atattctggc ggcgcaaact
     3181 gcccgttata ccaacccgga agagctggat gttctctggc cgatcatcgt cgaaaaagtg
     3241 gagcgcctgc cgccgcattt ggcgcaggtg cagcaacagg gacaacgaat caatcagctt
     3301 cgtaatttaa gctggtggaa gcaggccagc ctggaagagc tggaggatat tcgcattcat
     3361 ttgcgcggta tcatgcacct gatggaaaaa gacgcgacgc ctaaatttgg ttcgatacag
     3421 gtggatatta ccgaagatgc gaacctgatc cagacggaga cccgcaaaac taatatccgc
     3481 tcgattgatt tcaaactcta tcgccagcag gttcaggggg cgctggagcc gctgttccag
     3541 caaaacccgg ttctgaagaa aatccgcaac ggcgagccag tcacgcaaaa cgagctggat
     3601 gagctggcga agctggtgct gatccagaac cccaacgttg atattcgtgc gttgaaagag
     3661 ttttatccgc aggcaaccgc cagcctggat aaacttttgc gtaccatcat cgggatggac
     3721 agcgacgcgg tggaagtgcg ttttgcccag ttcgccgctg ataacagcct gaccagccaa
     3781 caactgcgct tcctgtcatt gctgaaaaac cacattcgcg attacggcac cattgaaatg
     3841 cggcagctct ttgaacagcc ttttacgcat atccacaacg aaggcgttac cgctgtgttc
     3901 cctgatatag cgcaaatcgc ccgcctgcaa aagatagtcg aagagctggg tgttgtgacc
     3961 gacgcagcga cggtataata cacgcatgac aagccccggc agcggggctt ttgtatttaa
     4021 aacaggatat aaacgctaac aatgattact ggcgacttaa aaagtaaaat cgacggacta
     4081 tgggaagatt tctgggtggg tggtatcacc aacccgctga ccgtaatcga acagatcacc
     4141 tatctgatgt attcccggat gctggatacc caggaacaac gcgacgaaaa gcgtaaacaa
     4201 atcgcgggta ttgattttaa accgcgtttt gcgccagaac agcaggagtt ccgtttcagt
     4261 cactatagca accttggctc ggatgagatg atggaagtgg tgcgcgatgg cgtattccag
     4321 catttccgtc agctcggcca ggccgatgct tcgaaggtga cgctgctggg caactttatg
     4381 aaagatgctc gtctggagat tgttaagccg tcattgctga ccaaagcggt tgaggtaatc
     4441 aaaaacctgc cattggatcg cggcgacacc aaaggcgacc tttacgaata cctgttaagc
     4501 aagctgacaa ctgccggaat caacggacag ttccgcacac cgcgccacat tatccgcacg
     4561 atggttgaaa tgatggagcc gaacccggcc cgcggcgaga cgatttgcga tcccgcctgt
     4621 ggcaccggtg gttttctggc aaccagctat gaatatctgc tggagaagta cagctcgctg
     4681 gaatccattc atactgagat tggcaccaac gaacgtggcg agctggaaga gcaaaaaatc
     4741 tttaccggcg atctgctgac accgtggcgt aaccatgtgg ataacaacat gttccacggt
     4801 tacgactttg acaccacaat gctacgtatc gccgccatga acctgattat gcacggcgtg
     4861 gatgcgcctg atatccacta tcaggacaca atgagccaga gtttcagcac aaacttcccg
     4921 caggccagta aaaacgcctt caacctgatt ctggcgaacc cgccgtttac cggttcactg
     4981 gacgaggaag atatcgactc cacgctgtcg gcaatggtga aaaccaaaaa aaccgaacta
     5041 ctgttcctgg cgcgtattct gcaaatgctg aaagtgggcg ggcgcagtgc cactatcgtg
     5101 ccgcagggcg tgctgtttgg ctctagcaag gcgcaccagt cacttcgcaa aacgctggtg
     5161 gaagataacc aactggaaac ggtgatcaat ctgccttctg gtgtatttaa accttacgct
     5221 ggcgtggcga cggcgatcct gatctttacc aaaggcggtc aaacggatga ggtctggttc
     5281 tacgatctac aaaatgacgg ctacagcctg gatgataagc gcaacccgat aaaagacaac
     5341 gatctgccgc atctgctggc aagctggaag cattaccgta ctttacgcgg gctaccggtt
     5401 gataacttta tgggtaagaa gttagcctcg ttgcttaaac agcagtaccc ggaagggatt
     5461 aatgctggcg ttgattttaa agatcgcacg caggcggcgt ttgttgtacc gaaagcggat
     5521 attgctgcgc agaaatacga tctatccatc aaccgttata aagaagtcgt gtatcaggcg
     5581 gaggaatatg aagatccgaa ggtgatattg aagcggttaa aggatctgga aaaagagatt
     5641 ctggcggatt tggatgagct ggaggggatg ctgtgagttc ggcgatggtg aagttagggg
     5701 atgttattaa tgttcactac ggaaaagcat taaaagctga tcagcgtgta tcgaatggaa
     5761 gtgtccatgt gtttggtagt tcaggaattg tcggcaacca cgataaaacg ctttgcagct
     5821 atcccacgat aattattggc cgcaaaggca gcgttggtgc tattacctgg gctccaagcg
     5881 gaggctggat cattgatact gcttattacg ttgagatcaa agataataat aagttagatc
     5941 tccgatactt gttttatatt ctgagcggaa ttgatctcac taagaaaacg attaccacaa
     6001 gtattcctgg attaaataga gacgatctct atgatacatt tattaaatta ccaccttttg
     6061 aagagcagaa acgaattgtg gatcttctcg ataaagctga gggtatacgc caaaaacgtg
     6121 agcaatccat caaactggct gatgattttt tgcgtgctac gtttgctaca atgtatggga
     6181 atcctattac aaaccccaaa aaatggcctg tccatcttat gggagaaatt attgaattta
     6241 aaggtggaaa tcaaccacct aaaagtgatt ttatttttga acctaaacag ggttatatta
     6301 gattagtaca aattagagat tttaaatccg ataaatatgc gacgtacatt ccgcaagaaa
     6361 aagcaaaacg tattttcgaa gttgatgatg ttatgattgc tcgttatggg ccacctgtgt
     6421 ttcaaatact tagaggttta tccggcagct acaatgtagc tttaatgaaa gcttcaccca
     6481 aagaaaatat taggaagggg tttatattct atcttctaca acttccggaa tatcatgatg
     6541 ttgttgtaaa aaactctgaa agaacagcag gacaaaccgg tgtaaatctt gaactattaa
     6601 acaaattcaa tgttcccctc ccacctatat actatcaaga tgagatatta gatagattag
     6661 ctagaattga aaaatttaaa gaaaaaatag aaatatcatt aaatcattta gaaatacaat
     6721 ttttatcact acagaagaga ttaataggtt tttaaatcct tagtatcgaa ataaacatta
     6781 atttattata ttaaattaag atattttagg aaattaaggc gtcactctta ggtaacgcct
     6841 ttttttacta tctcgccccc taagaaggtt atatcttact taatacctgt gactattttt
     6901 gatataatca taaatacaca ctaatttgca aaaattactt gacaacacct gagtattcat
     6961 agcttcatct atatgttttt tcaatatttt gagaagctct cccatatcgc gcttgattaa
     7021 tccaggctcc atataaggca tcattcttaa aaagaaactg aaatcataag caagatataa
     7081 ctcattgacc cccaccttga cctcaggaac ggtttcagag cgtcgtttat cccatccttg
     7141 agactgaaaa ctaataggag tgatatttaa tcgtgataaa acgccacctg agagttctgt
     7201 ttcattaatt atactcccat caggattaat taatttagcg tgatttaaga agtgaaaaat
     7261 aggaatgcgc gcattgcctt tgcatatatt tggcaaaaca tcattaacaa cacatcccca
     7321 atcataatat ccattttcaa atacaacctc tcggatgaga tctacacgct ccagtccaac
     7381 caatccttta gttgccatcg attccgattt ttttaccatc gaaatagcac catatcccac
     7441 aacaaactca gttgtactga ccccttcttc aattttctct gaatccctca catataattt
     7501 tcctttcgga tcatcagtta agaccaactc ataaagttca tcttttatca ttcggaataa
     7561 atgtgctgga atcctttttt tagtttcact cagcacctca aatatttctt tataggatgg
     7621 tgcctgaaca tattttaccg
//
LOCUS       DADLTD010000185         7629 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.186, whole genome shotgun sequence.
ACCESSION   DADLTD010000185 DADLTD010000000
VERSION     DADLTD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.186"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            16..192
                     /locus_tag="JN701_003342"
     CDS             16..192
                     /locus_tag="JN701_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY7365685.1"
                     /translation="MSALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGTNTLLA
                     CCCLLRPEEVNSEY"
     gene            182..355
                     /locus_tag="JN701_003343"
     CDS             182..355
                     /locus_tag="JN701_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY7365686.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(404..1476)
                     /locus_tag="JN701_003344"
                     /pseudo
     CDS             complement(404..1476)
                     /locus_tag="JN701_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1548..4292)
                     /gene="torS"
                     /locus_tag="JN701_003345"
     CDS             complement(1548..4292)
                     /gene="torS"
                     /locus_tag="JN701_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HAY7365687.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4375..5364
                     /gene="torT"
                     /locus_tag="JN701_003346"
     CDS             4375..5364
                     /gene="torT"
                     /locus_tag="JN701_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY7365688.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5337..6029)
                     /gene="torR"
                     /locus_tag="JN701_003347"
     CDS             complement(5337..6029)
                     /gene="torR"
                     /locus_tag="JN701_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY7365689.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6149..7321
                     /gene="torC"
                     /locus_tag="JN701_003348"
     CDS             6149..7321
                     /gene="torC"
                     /locus_tag="JN701_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY7365690.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7321..>7629
                     /locus_tag="JN701_003349"
     CDS             7321..>7629
                     /locus_tag="JN701_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase"
                     /protein_id="HAY7365691.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRSATAAQAAD
                     VISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDCRIAGLPDCRIA
                     G"
BASE COUNT         1854 a         1966 c         1967 g         1842 t
ORIGIN      
        1 cgtggagcct tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat
       61 aacctgttga ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg
      121 gtaccgggaa ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac
      181 agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca
      241 aaaaatcgcc gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc
      301 acgagaaaaa atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg
      361 tgaacatcac cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg
      421 gcgcttgtgg aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg
      481 catacattgc gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc
      541 agccagacaa cgcaaaagtt tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg
      601 ttgaggctac cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta
      661 cctgaattaa tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc
      721 cgatacaact ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca
      781 tcagcgcgcc atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa
      841 tcagcaaaac ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca
      901 tttttacgtc catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg
      961 gcatcaataa cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag
     1021 gaatatccag ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc
     1081 cgattaattc agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta
     1141 atagactgat gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa
     1201 ctgacctgtt gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
     1261 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
     1321 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
     1381 acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
     1441 ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc
     1501 ataccaaatg ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc
     1561 aggtctttct tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt
     1621 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc
     1681 attcccaaac tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta
     1741 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
     1801 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
     1861 gatacatcca gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga
     1921 ccgagcactt cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc
     1981 tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat
     2041 tgctgtgcca gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc
     2101 agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
     2161 acagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc
     2221 gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt
     2281 tttggcacgg gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
     2341 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
     2401 gtcagtccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
     2461 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
     2521 tcgccatcag tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg
     2581 ctcaacaggt tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt
     2641 acatcgtcgg caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
     2701 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca
     2761 ttcttgccac ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac
     2821 gattcgccag agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
     2881 tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
     2941 atcgccgcca gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt
     3001 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
     3061 tgttcacggt ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg
     3121 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
     3181 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
     3241 acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
     3301 tattgcccgc gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
     3361 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
     3421 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
     3481 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
     3541 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
     3601 attggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
     3661 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
     3721 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
     3781 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
     3841 atctcatcgg cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc
     3901 agacgccgcc ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt
     3961 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     4021 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     4081 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     4141 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
     4201 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     4261 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt
     4321 gagtcgcaaa gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc
     4381 gtactgctat ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg
     4441 ttgcgctggc atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa
     4501 cgcgcatgga aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg
     4561 aactatggta tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc
     4621 gaccagtgta aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt
     4681 cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat
     4741 gatcccctgg tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg
     4801 cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc
     4861 gataatgccg ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc
     4921 ccggtgcgta ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg
     4981 ttgcaggaga tgctggaacg ccacccagaa atcgacgtcg ttgccggaac ggccattgcg
     5041 gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt
     5101 tatctttcac atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc
     5161 gatcaaatga tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg
     5221 caatcggtgt ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac
     5281 cgtgaacata ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag
     5341 cacacatcag cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg
     5401 agtttatgac gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc
     5461 cgacgcgcag aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc
     5521 acaaatgcca ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc
     5581 tccagcgtat ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga
     5641 gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact
     5701 accagttcgc gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca
     5761 acaatacggt caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt
     5821 tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga
     5881 attaaatcta ccggctgatt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg
     5941 gaaacggtat acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca
     6001 tcttcaacaa taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct
     6061 gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc
     6121 actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc
     6181 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6241 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6301 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6361 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6421 gcgcaaactg gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac
     6481 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6541 agaaaacaac tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa
     6601 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     6661 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     6721 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     6781 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     6841 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     6901 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     6961 ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga
     7021 caccaatacc gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt
     7081 gaacgatatc aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca
     7141 gtgccacggc gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa
     7201 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct
     7261 gcaaatgaat gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7321 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7381 ttaacagtcg ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg
     7441 caagcggcgg atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
     7501 cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat
     7561 ccgtcgaaaa tgattgccgg attgccggat tgccggattg ccggattgcc ggattgccgg
     7621 attgccgga
//
LOCUS       DADLTD010000186         7595 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLTD010000186 DADLTD010000000
VERSION     DADLTD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.187"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            97..735
                     /gene="ytfB"
                     /locus_tag="JN701_003350"
     CDS             97..735
                     /gene="ytfB"
                     /locus_tag="JN701_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HAY7365692.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(719..1366)
                     /locus_tag="JN701_003351"
     CDS             complement(719..1366)
                     /locus_tag="JN701_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7365693.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIKVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            1578..2369
                     /locus_tag="JN701_003352"
     CDS             1578..2369
                     /locus_tag="JN701_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY7365694.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLECLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            2379..3221
                     /locus_tag="JN701_003353"
     CDS             2379..3221
                     /locus_tag="JN701_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY7365695.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            3231..4007
                     /locus_tag="JN701_003354"
     CDS             3231..4007
                     /locus_tag="JN701_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAY7365696.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            4017..5558
                     /locus_tag="JN701_003355"
     CDS             4017..5558
                     /locus_tag="JN701_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAY7365697.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGK
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHRKQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGVPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            5555..7595
                     /locus_tag="JN701_003356"
                     /pseudo
     CDS             5555..7595
                     /locus_tag="JN701_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
BASE COUNT         1880 a         1895 c         2010 g         1810 t
ORIGIN      
        1 tatgaatata ataaaggcgg gtttaccacg gacatcatag cgttagctta ttcataggct
       61 atgattgagg aacaagacgc ggagcaggag gaaaccatgc ccgggcgctt tgaactaaaa
      121 ccaaccctgg agaaagtctg gcacgcgccg gacaattttc gcttcatgga cccgctgccg
      181 ccgatgcatc gtcgggggat cattattgcc gccattgtat tggtggtcgg atttctgctt
      241 ccatctgatg atacgcccaa cgcgccggtg gtgacgcgtg aagcgcagtt agatattcag
      301 tcgcaatcac aaccgccgac agaagaacag ctgcgggcgc aattagtcac accacaaaat
      361 gatcctgatc aggtggctcc ggtcgctcca gaacctatac aggaaggcca gccagaagag
      421 cagccgcaaa ctacccaaac gcaaccattc cagccagata gcggaattga taatcaatgg
      481 cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc tgttccgcga ccacggactg
      541 cccgctaccg atgtctatgc aatggcgcag gtagaaggcg cgggtaagcc gctgagtaat
      601 ttgcaaaacg ggcagatggt gaaaatccgc cagaacgcca gcggcgtggt gacgggctta
      661 accattgata cggggaataa tcaacaggtc ctgttcaccc gccagccgga tggcagtttt
      721 attcgtgcgc ggtagatgaa gggtcaacca aaagccagct aaggattgtc tctttgatga
      781 ccttacgtct ggcttccagt gcctgggggg aaaccatact gatatgataa accaggccaa
      841 gcgtatgctg gttgatcaaa taatatccgc caagcgcagc aatattaata ttcacctgca
      901 atgggtcgat atctggctta aaaatattgt gtttcttccc ttcatccaat aaggattcca
      961 ttaactgcaa gtgcgcatgg ttaatttcta agagacgctg tgattttgca taatgcacac
     1021 ctttactttg attttcacta tgcacaattt ttaaaaacca ggggttggca atataataat
     1081 cccatgtaaa atcaataatt ttctcaatag ctttttgtgg cggaagatct gagaaatcaa
     1141 gggccttttc tttttgtcga atatcgttcc aggtatattc cagtacttta ataaataaat
     1201 tttctttatt accaaagtga tgataaaccg tctgtttact acaccccgca gccttaacaa
     1261 tattatcgac cctggcccct tcataaccat attcggcaaa ttcattaacc gcgcataaca
     1321 gcagcttttc cttcaggctc tgtacgttgc tggtatcaag atacataacg cttccccacg
     1381 acctcttcgt ggtgactatc ataacctgat ttgatttcag cttcagaaaa aagggcaaaa
     1441 acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgt catcgcatat caaccaacta
     1501 gatggttgac ctttttatcc tccagtcgca agctaatcat caccaaggaa acaaaattca
     1561 aaaaagagag ctattgtatg aaatccactc gtcctgttgc cgtgattacc ggagccgctc
     1621 gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg cgggtttaac ctgctgatta
     1681 acgatcgtcc cgatgccgac agcgtagaaa aactgcatat cacacaacaa gaatgtttgg
     1741 cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg tgatctatct ctgcatgagg
     1801 agatgctcga cgcggcgcaa aaccagtggg ggcggctgga gtgtctgctc aataacgcgg
     1861 ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga accagacagc ttcgatcaaa
     1921 acattgccat taacacccgc gcgccgttct tccttgctca ggcattcagt aagcgcttac
     1981 tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc gattattttc gtcagttcca
     2041 tcaacgccat catgctggcg atgaatcgcg gcgaatacac cattgccaaa accgccgttt
     2101 ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga acaaattggc gtttatgaag
     2161 tgcgccctgg tctcatcaaa actgatatga ctattccggc aacggcttat tacgacgaac
     2221 tgattgccaa aggtctggtg ccgtggggac gttggggtta tccggctgat attgcttcca
     2281 ccgtccgcgc gatggcggaa ggtaaactga tttacacctg tggtcaggcg gtcgccatcg
     2341 acggcggctt aagtatgccg cgcttctgag gctgaatcat gagagatctg ttacaacgcc
     2401 cggatttatt cagtatcaat accgcgacac tgggctataa aacgccgcta cccgccatta
     2461 ttgatgcctg cgccgcacgc ggtattggcg caattgcccc ctggcgcagg gagttgcagg
     2521 gtgaagatct ccagcaaatc acccgccagc ttgccgccag taacatgagc gtttccggtt
     2581 tgtgccgtag cacttactac accgcgccga cactggcgga acgcaaactg gcgattgatg
     2641 ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc cgcctgttat atgcaggtgg
     2701 ttggcggtct gccaacgggc actaaagatc tatatgaagc gcgagaacaa gtgaaacaag
     2761 ggattcgcca gttgctgcca cattcgaaag atgtcggcgt gccaattgcg ctggagccac
     2821 tgcacccgat gacagctgcc gatcgctctt gcctgtgcac gttacgtcag gcgctggact
     2881 ggtgtgatga actcgatccc gatggtgagt ttggcctcgg cgtggcggtg gatgtttatc
     2941 acgtctggtg ggatccggat ctcgccagtc agatcctgcg tgccggaaaa cgcatcctcg
     3001 cgtttcatgt ttccgactgg ttagtgccga ccactgatct ggtcaatgac cgtggtatgc
     3061 cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct ggttgaaaac gccggattta
     3121 atggtgctat tgaactggaa atattctcgc cttactggtg gcagaaagat attaacagca
     3181 cgctggatat tagcgtcgat cgcatcgcgc attattgtta aggaatgatc atgagtgacc
     3241 aacccgttct gttcagcaga gtggcggctt cctgccgcct gacattgaac cgtgaagata
     3301 aatgtcatgc tattaacgaa gagatgatcg aatctctcga ccattattta aatgaaattg
     3361 aaaacgacac cacattacga ttagtcgaat taacggcaac cggcgataaa ttcttttgtg
     3421 cgggcggcga tattaaatcg tggtcggcct attcgccatt agatatgggc agaaaatgga
     3481 ttaaacgcgg taatgacgtt tttaatcgcc tgcgaaacat accccaatta accgttgcga
     3541 atctcaacgg tcataccatc ggcggtggta ttgaactggc cttgtgctgc gatattcgta
     3601 ttgcccgccc cggcgcgaaa ttctctaacc cggaagtgat gctcggcatg gtgcctggct
     3661 ggatgggcat tgaacgtgtg cttaatcagg tcggtccggt ggtcggtcgc cagatgctga
     3721 tgctgggtaa acgcctgacg gcgcaggaag ctcaggcggc gaatttaatt gatgaagtgg
     3781 tagaaaaaga acaggtggaa agctggatgg cgaaccagtt agcgcagctg gaaaaatgcg
     3841 gtccggtggc actggcgcat attaagcaac tgattctggc actggaaaat aaacatgccg
     3901 attacccgca ccagctactg gccggattaa tgtccgccac tcaggattgc cagcaggcga
     3961 cgcgcgcctt tgccgaaaaa agtagcgtga gcttccataa tcaataagga aattgtatgc
     4021 gtaagataac aaccgccgag gcactggcgg cgcaaattca ggacggtgcg actattgcta
     4081 ttagcggtaa cggcggcggt atggtagaag ccgaccatat tctggctgct attgaagcgc
     4141 gtttcctgca aaccggacac cctcgcgacc tgacgctaat ccactcgctg ggtattggcg
     4201 atcgcgactg caaaggcact aaccgttttg cgcacgcaga gatgctcaaa cgcattattg
     4261 ccgggcactt tacctggtcg cccaaaatgc aggcgctggt aaaaaataac acgattgaag
     4321 cctactgctt tcctggtggc gtgattcagg cactgctacg ggagatcggc tccggacgtc
     4381 cggggctttt tacccacgtt gggctgggat cgtttgttga tccacgcaat ggcggcggta
     4441 agtcgaatga atgcactacc gacgacctgg tagaactgat tgaaatcgac ggtaaaacca
     4501 aacttcgtta tcgccctttc aaagtggatt acgcgatttt gcgtggcact tatgccgatc
     4561 ctcgaggcaa cgtcagcctt gaagaagaag cgattgatat ggatagctat tccatggcgc
     4621 tggcagcaca caacagcggc ggcaaagtgt tcgtacaggt acgcgatgtg ctggaagctg
     4681 gcgcgattga accacgccga gtcaaattac cgggcattct agttgatggc atcgttgaac
     4741 accgcaaaca gccacaaacc tatcttggcg gttacgacct gaccatcagc ggccaacatc
     4801 gccgtctgag ttctaacgac gctattgaac tggttagtca tccggtacgt cgcctgattg
     4861 cccgtcgggc agcacgcgaa ctggtagctg gcgcttcaac caactttggc tttggtattc
     4921 ccggcggcgt tccaggagta gcgctgcgcg aaggcgttcc ttaccaaagt ttatggctga
     4981 gcgtcgagca aggcgtacat aacggcatga tgctggatga tgcgtttttc ggctgcgccc
     5041 gcaacgccga tgccattatt ccatcactcg atcaatttga gttctacagt ggcggaggga
     5101 tcgatatcac cttcctcggc atgggagaga tggatcagta cggtaacgtc aacgtctccc
     5161 atctcaatgg caatctgatt ggccccggcg gatttctcga aattgcgcaa aatgcccgta
     5221 aagtggtgtt ctgcggcacg ttcgacgcta aaggcagcaa gatcgatata acaccagatg
     5281 gcttgcatat cgcccagtca ggtcaaatcc ctaaactggt taccaaggtg gaaaaaatca
     5341 cttttagcgc cgcctacgca cagcaaagtg gtcaggaagt gttgtatatc actgaacgcg
     5401 cagtattcca gttaacggca gaaggcgttg aattaattga aatcgcgccg ggtgtggaga
     5461 ttgagcgcga cattctgccg tatatggctt tccgtccaat tatcaagcac ccacgcctga
     5521 tggaaagtag cctgtttacg ccgatggagg atgcatgagt cagcttgatg acaccatcct
     5581 tgatgcgctg acgcacgtta ctttcccaaa gggttttgca caggcagagc ccgcatgggt
     5641 tgtcacggta gacggtgtta attacccact ctggcaaaca gatgccctgg tagtcggcag
     5701 tggtgcagca agattacgtg cggcagttga acttaaacgc cgccagcaaa atgtgctgat
     5761 cgctaccgcc gggttatata tggggacgtc ggcatgttct ggttccgata aacagacact
     5821 atttaccgcc gctaccgcgg gcaacggcga caacttcacc aaactggcag aggcactggc
     5881 gagcggtggg gcgatggatc acgacaccgc ttatgtcgaa gcggtaggtt ctctgcacac
     5941 tctaggcggg ttgcaatatc ttggtctgga attaccggaa gatcgctatg gcgcgattct
     6001 tcgttatcaa accgaccatg acgaagccgg gcgtgcaacc tcgtgtgggc cgcgtacctc
     6061 aaggttaatg gtgaaagtgc tgttggaaga agtacagcgc ctcgccattc cagtgttgac
     6121 cagtgcaaca gtgattaaac tgctgcatca gcgtgacgaa aacggcgaag accgcgtggc
     6181 aggggcaatc ctcgcgaccg gtcatcgcgc ccataaccca tgggggctgg caattgtgac
     6241 tgcgcccaat gtggtactgg caacgggcgg accgggcgag ctttatcgcg acagtgttta
     6301 tccacataaa tgcttcggct cgctggggct ggcgctggag gaaggtctga cgctaaccaa
     6361 tctgaccgaa agccagtttg gtataggcac gccgcgtagc acgtttccgt ggaatttatc
     6421 cggcacctat gtacaggtga tcccgtatat ctattccgtg gatgctgagg gtaacgagta
     6481 taacttcctc gcgattacta tcgcaccacc caggagctgg cttcaaacat tttccgtaaa
     6541 ggctatcagt ggccgttcca cgccactcgg gtgatggatt ttggctccag cttgttagat
     6601 atggcagtag cgcaagagca gcaatcaggg cgtcaggtat ttatggattt caatcgcaat
     6661 cctgaagcgg tgccgggtga cctgccgttc tcattagatc gactggacga cgacgttcgc
     6721 gcgtatctgg aaaataacga cgctctggca ccatcgccca tcgaacggct gcaacgaatg
     6781 aatccgctgt ctatctcgct gtataagatg cacggttacg atctcaccac acagcctttg
     6841 cagtttgcca tgaataatca gcatatgaat ggcggcattg aagtggatat ctggggacaa
     6901 acctccctgc ccggctgttt cgccgtgggg gaagttgctg gcacacacgg cgtcactcgc
     6961 cctagtggtg cagcattgaa tgccgggcag gtttttgctg ttcgtctggc acgttttatt
     7021 ggttgcacgc aaaagcggtc tgttgagggt gatgtcgcgc agctggtagc ctcatctctg
     7081 aatatagtca gagaagttat cgctcaggca ctggcaaacg agaacggaat ggcgctggta
     7141 acggtgaaag acaaaatcca ggcgcgaatg tctgattacg ccggatttat ttgtcatgcc
     7201 gataaagttc gtcatgccac ccgtgacgcc ctgctgctaa gtgagtttgt acaacaacac
     7261 ggtttaacca tcaatcacgc aggcgaaatt gccgaactgt ttatgtggcg gcatatgacg
     7321 ctgacttctg ccgccgtatt aacgcaattg acgcattata ttgacgcagg tggtggcagt
     7381 cgtggagcac ggatggttat tgatccacaa ggcaaatgcc tcccacaaac tcgtcgcggc
     7441 gcaaaagaag aatggcgttt tcgctctgaa cgtgctgaag acaaaaatca cagattaacg
     7501 attcaatatt cgcaaggttc ttttattacc gaagtgaagt ctttacgtat gcaaccgtgt
     7561 attaaaggta tttactttga aaaaaactgg ccaga
//
LOCUS       DADLTD010000187         7535 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLTD010000187 DADLTD010000000
VERSION     DADLTD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.188"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..244)
                     /locus_tag="JN701_003357"
                     /pseudo
     CDS             complement(1..244)
                     /locus_tag="JN701_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            196..1233
                     /locus_tag="JN701_003358"
     CDS             196..1233
                     /locus_tag="JN701_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HAY7365698.1"
                     /translation="MHRGFCVAHEPSILLPSVGAQLSNPDNITDNADRISDEWKAFDR
                     AIESNHGVPEAAARLKEHLRDFRNTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSV
                     DGKVSDADLTYLEEVANSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWS
                     LGFPPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTNLDDNWTLGLV
                     AQNLIPRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNR
                     QFAAIGAEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNT
                     YGAVAQFQFTF"
     gene            1594..5451
                     /gene="sigA"
                     /locus_tag="JN701_003359"
     CDS             1594..5451
                     /gene="sigA"
                     /locus_tag="JN701_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HAY7365699.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            5498..6080
                     /locus_tag="JN701_003360"
                     /pseudo
     CDS             5498..6080
                     /locus_tag="JN701_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6483..6883)
                     /locus_tag="JN701_003361"
                     /pseudo
     CDS             complement(6483..6883)
                     /locus_tag="JN701_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            6887..7462
                     /locus_tag="JN701_003362"
                     /pseudo
     CDS             6887..7462
                     /locus_tag="JN701_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2206 a         1709 c         1760 g         1860 t
ORIGIN      
        1 gacgacattt cttcgataac gtgtgttctt tctcctgcat cagcttatac agggaagtca
       61 ggagcggtct actctgcatt tgcctgaccg ccaggcgttc agacaccggt aacccgcgta
      121 tatcgtgctc tatggcgtac agttcaccga ttagtttcag agcttcttcc gccgtcgcgc
      181 ttttagtact gatatataca tcgtggattt tgcgtcgcgc atgagcccag catcctgctt
      241 ccgtccgtcg gcgcgcagct ctccaatccg gataatatca ccgacaacgc cgaccggatc
      301 agtgatgagt ggaaggcctt cgaccgcgcc atcgaaagta accacggtgt tcctgaggct
      361 gccgcacgcc tgaaagaaca cctccgggac ttccgtaaca cacatgccgc cgcacagctg
      421 ggcgtttctg ctgtggcggc ccttcccggt gatcgcctct ctgccgcact gatggttaaa
      481 tcacacggca ccgtcagtgt cgacggtaaa gtctctgatg ctgacctcac ttacctggaa
      541 gaggtggcaa acagcaccgg gcaggaggtg gacaaaagcc gcctgacatc acaggccttt
      601 gcccgcgcgg cactgatcac tgatgtgggt attgctctgg ctacggaact ggaaacggcc
      661 gggcagaaat ggtctctggg cttccccccc aaattccagc gcgtcgacct gttcaactac
      721 aacgtgctgg tcagaaatta tgacagcagc gccttcaaag gcgaccgcta ccacaacacg
      781 aaaaatggca tcaacgccga catcggtgcc agtacgaacc tggatgacaa ctggacgctg
      841 ggactggtcg cacagaacct gatcccccgt agtattgaga caaaagaagt gaacggtatc
      901 acggaaacct tcaggatccg cccgcaggtg acggccggtg tctcctggca caacgcgatg
      961 ttcaccaccg catttgatgt ggatctgacc ccggccagcg gtttcacctc cgacagcaac
     1021 cgtcagtttg ccgccattgg cgcagaattt aatgcctgga aatgggcaca gctgcgcgcc
     1081 ggttaccgtc agaatctggc cggtaacgac ggcagtgcat tcacggccgg ggtggggatc
     1141 tcaccgtttg atgtggttca ccttgatgtt gcaggcctga tcggcacgga caacacttac
     1201 ggtgcggtcg cacagttcca gttcaccttc tgagttccac ctgcaaaaca gcatgcacat
     1261 tatcgctgca tgttgtttcc atcatcaaaa gtctgtcgga catcgacaga cttaactccc
     1321 ccggataata cagaaaacct gtaaaggatt gaccggaaac aacaaagatc aaattttgat
     1381 cacaaaggtc aaaatacaaa caccaggtga tcaatatctg accaatcaat cacttgtgct
     1441 tatctttttt ttcattttgt tacatctgtc atacaaatat aactgacagt gattatcatt
     1501 atacccttat cagttacgta ccatgactga tagttccccg ttgtaattaa atgctatccc
     1561 ataaccacaa ctcagaaata tcggagttca cgtatgaata aaatttattc actgaaatat
     1621 agtcatatta caggtggatt agttgctgtt tctgaactga cccggaaagt tagtgtcggt
     1681 acatcaagaa agaaagttat cctcggtatt attttatcct caatatatgg aagttatggt
     1741 gaaacagcat ttgcagcaat gctggatata aataatatat ggacccgcga ttatcttgac
     1801 cttgctcaaa acagaggaga gttcagaccg ggtgcaacaa atgttcaatt aatgatgaaa
     1861 gatggaaaga tatttcattt tccagaacta cctgtacctg atttttctgc tgtttccaac
     1921 aaaggtgcaa caacatcaat tggaggtgcg tacagtgtta ctgcgactca taacggtaca
     1981 cagcatcatg caataacaac acagtcatgg gatcagacag catataaagc aagtaacaga
     2041 gtatcatctg gcgacttttc ggttcatcgt ctgaataaat tcgtcgtgga aacaacaggg
     2101 gttacggaga gtgccgactt ctcactttct cccgaagatg cgatgaaaag atatggcgta
     2161 aactacaacg gtaaggaaca aataattggc ttcagagcag gtgccggaac aacctcaacg
     2221 atattaaacg gcaaacaata tctgtttgga caaaactata atcccgactt gttaagcgca
     2281 agtcttttta atctggactg gaaaaacaag agttacattt ataccaacag aacccctttt
     2341 aaaaactcac caatttttgg cgatagtggt tctggttctt atctatatga taaagaacaa
     2401 caaaaatggg ttttccatgg tgttaccagt acagttggtt ttatcagtag taccaatata
     2461 gcctggacaa actactcgtt atttaataat attctggtaa acaatttaaa aaagaatttc
     2521 acaaacacta tgcagctgga tggtaaaaaa caagagttat catcgattat aaaagataag
     2581 gacctgtctg tctcaggagg aggggaatta acgctcaagc aggataccga tcttggcatt
     2641 ggcgggctta tattcgataa gaaccagaca tataaagtgt acggaaaaga taagtcttat
     2701 aaaggtgccg ggatagatat tgataataat accaccgttg aatggaatgt taagggcgtt
     2761 gccggagata atctgcataa aataggtagt ggtactctgg atgtaaaaat agcacaggga
     2821 aataacctta aaataggtaa tgggactgtc atccttagtg ctgaaaaagc cttcaataaa
     2881 atttacatgg ccggaggtaa aggtacggta aaaataaatg ccaaagacgc tttaagcgaa
     2941 agcggtaatg gcgaaatcta ttttaccaga aatggcggaa cactggatct aaacggctat
     3001 gaccagtcat ttcagaaaat cgcagcaaca gatgcgggaa caaccgtaac gaactcaaac
     3061 gtgaagcaat caacattatc acttactaat actgatgcat atatgtacca tgggaatgta
     3121 tcaggtaata taagcataaa tcatattatc aatactaccc agcaacataa caataatgcc
     3181 aatctgatct ttgatggctc agtcgatatc aaaaacgata tctctgtccg gaatgcacag
     3241 ttaacattac aaggacatgc gacagaacat gccatattta aagaaggcaa taacaactgt
     3301 ccaattcctt ttttatgtca aaaagactat tctgctgcca taaaggacca ggaaagcact
     3361 gtaaataaac gttacaatac ggaatataag tccaacaatc agatagcctc tttttcccag
     3421 cccgactggg aaagtcgtaa atttaatttc cggaaattaa atttagaaaa cgcaaccctg
     3481 agtataggcc gggatgctaa tgtaaaagga cacatagagg ctaaaaactc tcaaattgtt
     3541 ctgggaaata aaactgcata cattgacatg ttctcaggaa gaaacattac tggcgaaggt
     3601 tttggattca gacaacagct tcgctccggg gattcagcag gcgaaagtag tttcaacggc
     3661 agtctgagtg ctcaaaacag caaaataact gttggtgata aatcaactgt tactatgact
     3721 ggtgcattat ccttaattaa tacagacctg attatcaaca aaggagctac tgttaccgcc
     3781 cagggaaaaa tgtatgtaga taaagctatt gaactggccg gaaccctgac attaacaggc
     3841 acccctacag aaaataataa atacagcccg gcaatctata tgtcagatgg atataatatg
     3901 acagaagatg gtgccacgtt aaaggctcaa aattatgcct gggtcaatgg taatataaaa
     3961 tcagacaaaa aagcatctat tctgtttggt gttgaccagt ataaagaaga taacctggac
     4021 aaaaccacac acacaccgct ggctacaggt ttgctgggtg gctttgatac ttcttatacc
     4081 ggaggtattg atgctcctgc tgcctcagcc agcatgtata acaccttatg gagagtaaac
     4141 ggacagtcag ccctgcaatc attaaaaacc cgcgacagtc ttttgttgtt tagtaacata
     4201 gagaattcgg gtttccatac tgtgacagta aacacactgg atgccactaa tactgctgtg
     4261 attatgcggg ctgatctgag ccagtctgta aatcaatcgg ataaactcat tgttaaaaat
     4321 cagttaaccg gaagcaataa cagtctgtcg gtcgatatac agaaagtggg aaataataac
     4381 tcaggattaa acgttgacct gataacagcc ccaaaaggaa gcaataaaga gatatttaaa
     4441 gccagtactc aggccatagg tttcagcaac atatctcctg tgatcagcac gaaagaggat
     4501 caggaacata ccacgtggac cctgaccgga tataaggtgg ctgaaaatac agcatcttcc
     4561 agtgcagcaa aatcgtatat gtccggtaat tacaaagcct tcctgacaga agtcaacaac
     4621 ctgaataaac gaatggggga tctgcgtgac accaatggcg aggtcggtgc atgggcccgc
     4681 atcatgagcg gagcaggttc agcttctggt ggatacagtg acaactacac ccatgtgcag
     4741 attggtgtgg ataaaaaaca tgagctggat ggacttgacc ttttcactgg tctgactatg
     4801 acgtataccg acagtcatgc cagcagtaat gcattcagtg gcaagacgaa gtccgtcggg
     4861 gcaggtctgt atgcttccgc tatatttgac tctggtgcct atatcgacct gattagtaag
     4921 tatgttcacc atgataatga gtactcggcg acctttgctg ggctcggaac aaaagactac
     4981 agttctcatt ccttgtatgt gggtgctgaa gcaggctacc gctatcatgt aacagaagac
     5041 tcctggattg agccgcaggc agaactggtt tatggggccg tatcaggtaa acggttcgac
     5101 tggcaggatc gcggaatgag cgtgaccatg aaggataagg actttaatcc gctgattggg
     5161 cgtaccggtg ttgatgtggg taaatccttc tccggtaagg actggaaagt cacagcccgc
     5221 gccggccttg gctaccagtt tgacctgttt gccaacggtg aaaccgtact gcgtgatgcg
     5281 tccggtgaga aacgtatcaa aggtgaaaaa gacggtcgta ttctcatgaa tgttggtctc
     5341 aacgccgaaa ttcgcgataa tcttcgcttt ggtcttgagt ttgagaaatc ggcatttggt
     5401 aaatacaacg tggataacgc gatcaacgcc aacttccgtt actctttctg ataacagccc
     5461 gggccgcgtt tgcggccctt cttctaccgg agagaatatg tattaccctg tgacagacta
     5521 tatcgctctt gctctcatta ttagctttct ttttctgaca ttatttatct gcctgttatg
     5581 ccttaaacat gagcgaataa aaaaagaaac tatcaggcaa aaaaaatgca catattctgg
     5641 agcatggctg gaatgcaact gagttctcat ggttccgata cggacagtat aacgaaacgg
     5701 gtatttacat ctcgatcgag aagaccatta tcattacaat caccgtttct ggtgggtgct
     5761 ttaaaaaaga atacagcatt gtctcccaca tgctggtcac tgacaccata acagaggcca
     5821 ccctgtacga aaacgggctg tatacgcgcc acatcagact cagtcgccca gtatctgaca
     5881 gcaaataccc cttgccaccc ggatctcagc tcattaaaaa tatgacctta cgactgcgcc
     5941 tgcaggatca gcaggaagaa acatccgtga cattatttca gggaaaaatg agtaccgacg
     6001 gaaataacta ttacatcatc aaaggtaaag taagctcagt acttctgtta ttaaaaatgc
     6061 tgcagattaa tcacgcctga tctctgtccc ggtattttag cccgaggtat attctgaaat
     6121 atcattcatc atgaccggcg tttatacaaa cgccggtcat tcgctaacag cttcaccgtt
     6181 ccttccccca tccaggttcc gcatgagcgt gttgtaatgt tcataagcgt attatcgtaa
     6241 gcttccgggg aactcatatt gacgcgtttt tcaggcgcgc gtcatcggcg gcgcatacga
     6301 acgatcgtgg tttgataacg gttgatttat tttgttgcac aggggcgatc gccaggcagg
     6361 taccggaggc gggctctgaa gtactgagta gggtgatcgc gtcaggccgc gaagtactga
     6421 ctcggggatt aatagcaggt gatgagtaag cgtacagcga gggccgtatt gacggggatg
     6481 tgttattcag ctggcagtgc tatgcgccac ggaagcagtt cgctgacccg gttgaccggc
     6541 cagtctgcta tgacgccaag cacatggcga aggtagcttt ctggatccac gtcattcagt
     6601 ttgcacgtcc cgatcaggct gtacagtagc gctccccgct caccaccatg gtcagagccg
     6661 aagaacagga agtttttacg acccagactg accgcccgca aggcattttc agcgatgttg
     6721 ttgtcgattt ccacccagcc atcgttcgca tagtacgtca gtgccggcca ctggttaagt
     6781 gcgtacgcga acgccttcgc caactctgag tgtcgcgaca gggtcttcat cttttccgca
     6841 accagctttc cagggatttc aacagcggtt tcgtttttcg ctgacaccat ggagggagtg
     6901 gttaaaagag caacgagaaa gtggtaatta taacgccagc cggatatggc gggaaatggt
     6961 ggcccgggga tttacaggca gtgaaaccat cgtcagggat gcggttgcca aatggcgtaa
     7021 aggctggaac ccaccggtta ctactgccgt cagacttcct tcagtgtccc gggtaagccg
     7081 ctggttgatg ccctggagaa taaccaggga tgaagaaaat tatgcttccc gatttattag
     7141 tctgatgtgt gaaaaagaac ctgagctgaa aatagcgcag caactggcac tcgagttcta
     7201 ccgtattctg aaaacccaaa ataaatcaca gctaagcagt tggttcactc gagtccacga
     7261 aagcggttca gcagaatttc ggcgcgtggc ggcggggatg gaagctgatg ctgcagctat
     7321 atgtgaggca atcagtagtc gctggagtaa tggtgttgtc gaaggtcatg taaatcgcct
     7381 gaagaagatg ctgaaacgcc agatgtatgg tcgagcagga tttgaactac tcaggcagag
     7441 ggtaatgagt ccactggcat gaaaatctcc acccaggttg cggaagagcc acttttcatc
     7501 cgtcgttgac agttataccg ccagtcagaa atata
//
LOCUS       DADLTD010000188         7521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLTD010000188 DADLTD010000000
VERSION     DADLTD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.189"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..587
                     /locus_tag="JN701_003363"
     CDS             <1..587
                     /locus_tag="JN701_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7365700.1"
                     /translation="NQLADKQTIKQTLFARQPAKIHKPYPTNSKIIMGDVLNHAALKQ
                     AMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVLSLGIYDEVPGKFGEWNNA
                     VIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELTSRNEPFKGTIVSRKSV
                     AALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            897..1625
                     /gene="mpaA"
                     /locus_tag="JN701_003364"
     CDS             897..1625
                     /gene="mpaA"
                     /locus_tag="JN701_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY7365701.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1600..2565)
                     /gene="ycjG"
                     /locus_tag="JN701_003365"
                     /pseudo
     CDS             complement(1600..2565)
                     /gene="ycjG"
                     /locus_tag="JN701_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            2684..3190
                     /gene="tpx"
                     /locus_tag="JN701_003366"
     CDS             2684..3190
                     /gene="tpx"
                     /locus_tag="JN701_003366"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY7365702.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3234..4775)
                     /gene="tyrR"
                     /locus_tag="JN701_003367"
     CDS             complement(3234..4775)
                     /gene="tyrR"
                     /locus_tag="JN701_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY7365703.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4923..5984)
                     /locus_tag="JN701_003368"
     CDS             complement(4923..5984)
                     /locus_tag="JN701_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY7365704.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5981..7378)
                     /locus_tag="JN701_003369"
     CDS             complement(5981..7378)
                     /locus_tag="JN701_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY7365705.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1859 a         2077 c         1810 g         1775 t
ORIGIN      
        1 ttaatcaact ggcagataaa caaaccatta aacaaacttt atttgcccgt cagccagcaa
       61 aaatccacaa accctatcca actaatagca agattattat gggagatgta ctgaatcatg
      121 cagcactgaa gcaggccatg cagggacagg acgttgtgta tgccaacctt acgggggaag
      181 atttagatat tcaggcaaat tcagtgatcg ctgcgatgaa agcctgtgat gttaaacgtc
      241 tgatctttgt tctttccctc ggtatttatg atgaagttcc aggaaaattt ggcgaatgga
      301 ataatgcagt tattggtgag cccctaaaac cattccgcag ggctgcggat gcaattgagg
      361 cttctggact ggaatataca attcttcgcc ctgcctggct gactgatgaa gatattattg
      421 attatgagct tacctcacgc aatgaaccat tcaaaggtac gatcgtatca cgtaagagtg
      481 tcgcagcatt aatcacagat atcatcgata aacctgagaa acacattggt gagaatatag
      541 gtatcaatca acccgtgact gatggtgaca agccattatt catgtaaatc aattacatag
      601 ttaccatcgt aatgtactat gtgcagtacg atttattaaa cttattaagg tgaactgatc
      661 aatccgatgc gattaacatg tgtagaacca gtaagaataa aatacgccat gtatataaca
      721 ttacgactta catctaagtc agcataattc taatatctgc acatcaataa aaatgcaaac
      781 aacttccggc aaaaaaagtg atacaagatc gatgacatag cgttgaagaa aaggatttga
      841 taatccgcaa aatttgcaca acactgcctg taatacttcc tgaaccagag acgatcatga
      901 ccgtaacccg cccacgcgcc gaacgcggcg catttccgcc cggaacagaa cattacggac
      961 gttcattatt gggcgcgccg ttaatctggt ttcctgcccc tgcagccagc cgcgaaagtg
     1021 gtttgattct ggctggcact cacggtgatg aaaactcttc ggtcgtcacg ctctcctgtg
     1081 ctctgcggac gttgacacct tctttgcgcc gccatcatgt ggtgctgtgt gtgaatcctg
     1141 acggctgcca gctggggtta cgggccaatg ctaatggtgt ggatttaaac agaaactttc
     1201 cggcggcgaa ctggaaggaa ggtgaaacgg tttatcgctg gaacagcgcc gctgaagaac
     1261 gtgatgtcgt attactgaca ggtgacaaac ctggctcaga acctgaaacc caggcgctgt
     1321 gccagcttat acaccgcatt caacctgcct gggtggtctc attccacgat ccactggcct
     1381 gtattgaaga tcccagacac agcgaattag gcgaatggct ggcccaggcg tttgaattgc
     1441 ctctggtaac cagcgtcggt tatgaaacgc ctggctcttt cggcagttgg tgtgcagatc
     1501 tcaacttgca ttgcatcacc gcagagtttc cgcccatctc ctccgacgaa gccagcgaaa
     1561 aatatctctt tgcgatggca aacttgctgc gctggcatcc taaagatgca attcgcccgt
     1621 cgtgaactga agcgccggtt ccacatctac cgccagccag gtcggtccgt caagatcggc
     1681 gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg gcacgagagg tacacaacat
     1741 gcagcccagc atcagactga aaccttgtgc acgcgcttca gtcgccagcg ccagcgcttc
     1801 cgtcagacca ccggttttat cgagcttaat gttaaccatc tcatagcgcc ctttcagcgc
     1861 cttcagattg ctacgtgtat gacaactctc atcagcacaa atcggcaacg gatgaataaa
     1921 attctctagc gccgcatcgt cctgcgcagg aaggggttgt tcaagcatcg caacgcctaa
     1981 atccgccaat agctggcaac gcgccgccaa cccttctgca cgccaggatt catttgcatc
     2041 aacgatcagc gtcgcatcgg gcacagctgt gcgaattgcc accatccgct cactaataag
     2101 atggttatcc agcttcactt tcagtaattt cgcgcctgcc tgccagagtg ttgatgcact
     2161 attggccatc tgatcaggcg taccgatgac taccgtctgt gcagtgataa ctgtctcggg
     2221 aagcgttatg ccgatcaaat cagccagcga ttgctgctgt tttcgcgcgg ccagatccca
     2281 caatgcacaa tccagcgcat tacgtgctgc gccggcaggg agaatttttt gcaactcctc
     2341 ccgtgtcagc cctttctcta attgcgacac gacgctcata atttgcgcca ttaccgaggc
     2401 atcactttct ccataacgcg gatacggcgt gcattcgccg gtgcctttaa taccctcttc
     2461 ttccagttca accaccacca cgcgcgcttc actgcgactt ccccgggcaa tcacaaacgg
     2521 ggtatgtaag ggctaggctt cctcaaatac cttaacggtt ctcatcttta ctccttgttg
     2581 tcgcggtggt ctgcggatta agcgcaaata gcgtttgctg tgttattgac agttagcata
     2641 aactaggtgt gacgttaact atatgtaaac aggaaagata atcatgtcac aaaccgttca
     2701 tttccagggc aacccggtta cagtcgccaa ttccatcccg caggcgggta gcaaagcgca
     2761 gacttttact ctcgtggcaa aagatctgtc tgacgtcacc ctcggtcagt ttgcgggtaa
     2821 acgcaaagtg ctgaacattt tcccgagtat tgataccggt gtttgcgccg catcagtacg
     2881 taagtttaac caactggcaa ccgagatcga caacaccgtt gtgctgtgta tttctgccga
     2941 tctgccgttc gcccagtctc gtttctgcgg cgcagaaggt ctgaacaacg ttatcaccct
     3001 ctccactttc cgtaacgctg aatttctgca agccttcggt gtggcaattg ctgatggccc
     3061 actgaaaggt ctggcagcgc gtgccgttgt ggttattgac gaaaatgaca atgtgatttt
     3121 cagccagctg gtggatgaaa tcaccaccga gccggattac gaagcagctc tggctgtact
     3181 gaaagcataa gttaatatgc ctgatggtgt tgcaccatca ggcatattcg cgcttactct
     3241 tcgttcttct tctggcttaa gccatattcc cgcaacttat tggcaatcgc ggtatgtgaa
     3301 acgccgagac gttttgccag tttgcgcgtg ctgggataat tgcgataaag ctgggttaat
     3361 accgagcgtt caaaacggct ggtgatttcg tccagcgaac cttccatcgc atcttcgccc
     3421 acggctaccg ttgcggcgtc ataatccggc aacaaaatat cctgtggacg cagctcataa
     3481 ccgtccagtt gtgtcagtgc gcgatatata gcgttcttta actgccgcac atttcccggc
     3541 cacgcataac gcgtaagtac agtattcagg tcagcggcca gtttcggacg cggcacgccc
     3601 tgctcgtcgg caaagcgggc gacgaacagc tcagttaacg gcatgatgtc ctgcggacag
     3661 tcacgtagcg gcggcagatt gagcgtcaac acgttcagac gataatagag atcttcacgg
     3721 aacatgcctt tttgcaccag ttcgaccaga ttcttctgcg tagcgcaaat cacccgcaca
     3781 tcgacatgca cctcatggtc ttcgccaacc cgacggaaag tgccatcatt aagaaaacgc
     3841 agtaatttcg cctgcatccg tggtgacatt tcccctattt catccaacag caccgaacca
     3901 ccgttcgcct gctcaaagaa tcctttcttc ccttccggag catgaccaaa cagttcactc
     3961 tcgaccgcat cttccggtat agacgcacag ttcagcgcca ggtaaggttt gcccgctctg
     4021 gggcttgcct gatggcaggc gtaggcaaag agatctttac ctgtacctgt gtcacccgta
     4081 atcagcagcg gcgcgcttag catcgccagt ttctgcgcct gttcgacaac atgcttcatt
     4141 ttcgggctga cggcgacaat ttgactgaag gcgctgacgt cctgggcggc gacattttgc
     4201 aactggcggc ccatacgaat cgttgatcgc aacatcacca ccgcaccggt caggacgtgt
     4261 tgatcatttt catcctgaag ataaacaggc gtaatctcca tcaggaaatt ctgcccatta
     4321 ataacgacat gctcgttatg cgaatcttgc ggttcgcttt ccagccaacg taaaaaatta
     4381 aagccgttaa tcaattgtgc ggcggtatgg ttgcgcaggc gatccaattt ttgcccaaaa
     4441 agctgacagc tcgccgggtt cgccatatcc actttgcttt tcatatcgac agagagcaca
     4501 ggttcgggca acgcctccag taacgcgctc aacgccagat gctcacgttc ggaaggcatc
     4561 cacgggacag tacgcacatc ggtaacacct gcaatacggc gtatttcggc catcagactg
     4621 ctgaaactct caaactccag ttcagcaaaa ttgaggtaga ttcgcccaat gggatcaatc
     4681 tcaataccgc gtaaatcaat gcctcttagc acgagtagat cgagtaattc gcgggtcaga
     4741 ccgagtcggt cttcacaaaa gacttccaga cgcatgggaa ccttcacctg aaaaaagaac
     4801 aattaatatg atgatatgac actatttgga tagcaggaag gtttctgtca acaatcattg
     4861 acacaaagac ggaaaaacgg gcacttcggc gtaaagatat ccggtcagca cccgatattg
     4921 cattattttt cgctgggcgt tttgcctttt tgcagcgttt ctttcacctg accgataagc
     4981 tgacgacgga aatccccgag gcgaggtttg tcatcgtcaa tccacggcag cgggcggcaa
     5041 agctccatag ctttaatccc gagtcgtgcc gtcagaagtc cagcaccaat tccctgagct
     5101 gcgcgggtag acaaacgagc agcgagatct tgcgacatcc agtccatccc cacttcgcgc
     5161 accagttcgc tggctccggc aaaagcgata ttcagcaata ccagcttaaa cagacgcaaa
     5221 cggctgtaat accccagttc aatgccatac agcgtggcga tgcgattaat taaacgcaga
     5281 ttgcgccagg cgataaacgc catatcgacc aacgccagcg ggctgaccgc aatcatcaac
     5341 gttgattccg ccgccgaacg gcagatttcg cgccgcgcct gggcatctaa aactggctgg
     5401 accaaatgcg catacaaact gaccacttca cggtcgtttt gcgtttcatg gattgaggca
     5461 taccagcgtt gcagcgctgg atgagactga tcaatacccg cctgctgcgc cagtttttcg
     5521 caaaatgcgc ggcctttgcc cgtgccgtgg ctgtgcaaca aatcgcgcgc ttcgtcgcgt
     5581 tcatgggcgc gctgtcgcaa gcgccataag cgccgccact ctgttaccac agaacctacg
     5641 ccagcgccga taatcaatgc cccagcggca catccaccca gcgccaccca gtcctgagtt
     5701 tgccaggcat tcattgtcca ctgtacaccc tgcccgacaa cgcttgcgcc aaacagagcc
     5761 agcccgccca tcaccatttt gcgccacagg ctgcgtttcg gacgtaacgc tgcatccatt
     5821 accgcttcaa cttgcccctc ttcttcaggc gcttcatcga gcgtggccgg ggcaaaattt
     5881 tgcgcctgat tttcgtcaaa ggtctgctgc gccctgaatt ttggattctg atcgacctcc
     5941 agcggaccgt cgaaatcaat acgtggtttt aacggttcgg tcatcgcaat ttatctccta
     6001 ttaaaaattc cagcgcggca tcaagacgaa tatgcggcag cggtttgtcg acatccatca
     6061 cctgcgggcg aaacgcttca aactggaacc cttgtttgtc ccagaacgcc tgaccgggca
     6121 aacgcgcagg aacttcaccg ggataaacgg tgagtggcgc accatcgctg agccggttac
     6181 cgcgcaatgc cggaattttc tcgccgttga cgtcaataat gccgctggtg gtcgcctgca
     6241 ccgaggccag ccccaggcaa tccatactaa tcccttcaaa cgccgcattt tgccaggcat
     6301 cctgaatcag ttgttgcagt aatgaaacca tattggcgtg ctgatcgatg gtcacatggt
     6361 ccgctttagt ggcagcaaac aatagcttat cgataaccgg cgaaaacaaa cgccggaaca
     6421 aggtacgctg cccgtagtgg aaactttgca tcagctgcgt cagtgccaga cgcatatcat
     6481 taaatgcctg tggcccactg ttgagaggtt gcaggcaatc aaccagcaca atctggcggt
     6541 caaagcgcag aaaatgattc ttatagaacc ccttcaccac cttctcgcag taataattaa
     6601 accgctcgcg cagcattccg gcattggtgt gcttatcggc ctgcgccagt ttggactcgc
     6661 cccaggtatc gacatccggc cacgggaaga attgcagcgc gggcgcacct gccatatctc
     6721 ctggcaagac aaagcgccct ggctgaataa agtgcagccc ctgctgttta cagtggtgga
     6781 gataatcggt ccacgcggcg gcaatgtctg ccagccggtt ttcgtcggca ggtgctagcg
     6841 ggtccagccc ttcgcacatc attcgccatt tgaccgacca ttcgccgcgc tgaccattga
     6901 gtaagcccgt catctggcgt gaccagctta aatagtcctg cgccagcatc ggcaggtcga
     6961 gcaaccattc gccgggataa tccacaatct ccagatacag cgtggaggtg tccttgaagt
     7021 ggcgtagcag cgaatcgttc gatttaaaac gtagcgccag gcggatttca ctgacgccgc
     7081 gcgttggcgt cggccaggcg ggaggatcgc catataactg cgccagtcct tcatcatatg
     7141 tgaagcgcgg aatgccaaag tcacgctgag gaatgcgttt tacgcccagc aggcgctctt
     7201 cacgcaccgc gcttaacagc ggcaagcgtg ctccggcgtg aatattgagc aactgattga
     7261 ccatagcagt gataaacgct gttttgccgc tgcggcttag tccggttacg gcgaggcgca
     7321 gatgtctgtc gacaccccga ttcaccagcg cattaagttc atttttaagt cgcttcattg
     7381 ctgtcctgtg ctgctctgaa gaccagaaaa aaatcattcg taaggatatg aatgtggggg
     7441 ccaggccatt gattatcaac tgcccgtgtt gtcatcgacg ggcgaactcc ataagatgtg
     7501 cagcattcac gttcggacgg a
//
LOCUS       DADLTD010000189         7472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLTD010000189 DADLTD010000000
VERSION     DADLTD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.191"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..339
                     /locus_tag="JN701_003370"
     CDS             <1..339
                     /locus_tag="JN701_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7365706.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            714..1148
                     /locus_tag="JN701_003371"
     CDS             714..1148
                     /locus_tag="JN701_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY7365707.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1159..1800
                     /locus_tag="JN701_003372"
     CDS             1159..1800
                     /locus_tag="JN701_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365708.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1797..2147
                     /locus_tag="JN701_003373"
     CDS             1797..2147
                     /locus_tag="JN701_003373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365709.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRLETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2841..3452
                     /locus_tag="JN701_003374"
     CDS             2841..3452
                     /locus_tag="JN701_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365710.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGM
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELSVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3684..3869
                     /locus_tag="JN701_003375"
     CDS             3684..3869
                     /locus_tag="JN701_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365711.1"
                     /translation="MTPREIALLTIAKLEHGGHQLTQADQREIERSVNADIARRDRFR
                     EMMRAPAYQWKKPAPRR"
     gene            3990..4532
                     /locus_tag="JN701_003376"
     CDS             3990..4532
                     /locus_tag="JN701_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HAY7365712.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4525..4884)
                     /locus_tag="JN701_003377"
     CDS             complement(4525..4884)
                     /locus_tag="JN701_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004202368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365713.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5318..6142
                     /gene="dinD"
                     /locus_tag="JN701_003378"
     CDS             5318..6142
                     /gene="dinD"
                     /locus_tag="JN701_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY7365714.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6434..7051
                     /locus_tag="JN701_003379"
     CDS             6434..7051
                     /locus_tag="JN701_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY7365715.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7048..7472)
                     /gene="ligB"
                     /locus_tag="JN701_003380"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7048..7472)
                     /gene="ligB"
                     /locus_tag="JN701_003380"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         1997 a         1834 c         1908 g         1733 t
ORIGIN      
        1 cagacggagg cgtttttgaa tgcctatggg ctgagtcgat tcgcgccgct gggttatgac
       61 ccgcgcgatt tacccatccg tgatttagcc gggtacagga agaaggggaa ccacgacggc
      121 gacccgataa ttttctacac cttcccggca gcgtttgagc aggagattgc aaagggcttc
      181 aataccaaac agtttgccga agttctaaag aatgcaggaa tgcttacacc accaacgagc
      241 gggcgaggct atcaggggcg agtaagggag gatgggcgac agattcgcgt ctatgtactc
      301 aatttcatgg cagaggaaag cagccagcca gaagaataat tatttcttca catgcgtagt
      361 tttagtgttg tctcggttgt ctttgttgtc tcaatttgta attgagattg atttataagg
      421 attttatttt aatttctgag acaacaccga gacaacattt agccattttg agacaacagg
      481 gcgaaagtgt gtttttagtc tggcaggcag tgagacaaca tttatgccgg gtgagacaac
      541 acgaaattgc aaatgttgtc tcagcaaaac ccagacgtgg cgcggcctgt agagccgtga
      601 gacaacaaag acaacagaga caacactatt tttgcttact atgaaaaaaa atcggcaggt
      661 gtcccacatt aagaaatcgt cattagcaaa actaataatc cagtgagggg aaaatgacag
      721 cacaaatttc agcatacggg cggctggtgg cagatcccca gacccgcacc acaacgaacg
      781 gtaacaacat ggctatggcg cggctggcgg tgtctctccc ctgtaatgcg gcagaggcgg
      841 gagagtcaac cttctggctg ggcgttattg cttttgggaa gcaggccgat gcgctggcaa
      901 agcaccagaa aggcgacctt gtgagcgtgg caggcaatat gcaactcaac cagtggacag
      961 ggcaggacgg cggtacacag caggggtatc aggtagtagc cgacagtgtg atcagcgcca
     1021 gaacggtacg ccccggcggg aaggcaggac agcggggaca ggcaacggac gcgctgagac
     1081 gtgcgcagca gccatcccgt gatgaatacg accaacgccc accattcgac gatgaaacgc
     1141 cattctgaga gggaagatat gagtacgcaa cccatcatta ttgagttcgg cagctatcgc
     1201 tggcccagac atcttgcaat acatgaagca gatcatgcga tagccgcatg gtttttcggc
     1261 atacaggagg tagaaattgc gttatcgggt aataaccgag tggcaaaaac ctaccttagc
     1321 ggcgatgttg atgaatgtgg agctgtcgcg gtatggcacg ccaactatcc acggaatgtg
     1381 gctgaggtta agtcttacac cctctcgaat gatgacacca ggttacgtct gatagaccag
     1441 gtatgccggg ctatggttat ctctctggct ggtatcgtcg cgcaatcacg ctatacgggg
     1501 gatgatatta acgcactgat ggaaacgtcc gggaaagcag acatgaaaaa tgttcgtgat
     1561 ttatcagagg tataccgggt ggctggtggg gaggatgctg atatacagag aatatccctt
     1621 tcccgtgctg aggcgctgat tgccctgaaa tggtgggatg ttatcgcgct ggcgaaaata
     1681 ctggagaacc gtaaccatat gcctgccagc aacttccagc gcattatggg tgatattgac
     1741 aagcctgtac caaagcctat gacgctggct gaactggatg aattggtggg tgaagaatga
     1801 agccatacat tctggcagcc ggcctgatct cctcggcacc ggcactatct gctatccatt
     1861 gtgggggctt taccatacag ccagaccgtt atcggacaac gattaacggc gatgtggttc
     1921 atattgttga tgcgcaattt tacggcgcac cgcgagactt tagccaggcc gccataaagt
     1981 tattgccgga tgccattacc actaccgata ggcaataccg cctagagacg cagggaggca
     2041 atgccttgct tgagttgatg accagagaaa acccgccacg tgtgcttaat cgtgagcgct
     2101 gcgatgccgg gttaacaagt ttcggttggt acacttctgc gccgtgatgt tcgttccgag
     2161 cgcagcctaa aatattggac tgttcttgac ggatttaaaa ctctaatgga gccaagtcct
     2221 tgccagtagc gggctaggag cctaattgtg attcaggtcg tcaaaaatat tcaggtttgc
     2281 ggcattggtt cgcagtacgc agtccggtac gcggcaaaac tgcgcattta acgtgcgtac
     2341 cacctgacga attctaacgc cggcattgca aacagatgcg tgcgcaaaat ggctaagacg
     2401 tctgatattg ctggctttgg ctcaatcagt ttggaggtgg cggtatcagt acgatgctaa
     2461 tttttgctaa ggttttagta cgcaactcag gtacgcactc tcaccagata cgaaaccgat
     2521 tcgcagttgc gtaccagcct gtaaggcgca ttatcgctgg gtttctacag tcgaatgcag
     2581 tacgcaaagt ggaaaatcct ccgaatacga aacccgagca ttgcgcagta aattgcgaat
     2641 tgcgcagtta acaaaatatc tccgcaaaac gaaacggata tcaatgcgca aaccatcctc
     2701 aacccccgcg cggttactgg ctttattgat gtgaaattgc aaaattggtg cgcaatttac
     2761 agtaaaatac gcatattatt tcgtattcaa tttgcgcacc gttcgcagtt aatgcgcagt
     2821 aattcgcagg aggccagcca gtgagagaca gaacgcgcag ctataccacc gacctgcccc
     2881 gtatagggtt gccattcctt gcgaacatgc gtagcaggct ggcggatgct gaaccgggta
     2941 cgcagcttta tacccagacc gaaagcggaa tgttgtatgc cttccggcaa gccgacagct
     3001 acgccatgac aatcaacggc gtgacgcggg ctattcgcac cacaaccacg aaggccgggt
     3061 atggcgtccg ggaatggtac gcttgccccc actgtatgaa gcgggctgca aagctgtata
     3121 tcggcaagaa agacattggc tgccgggcgt gctggaaact tcactataaa agccagagtg
     3181 ccgacatact ggacaggatg cgcatgaaga tacgccagca gcgatgcgcg atatggggca
     3241 gcaatgacct tgccaataat ctgtttaatg acattcgcat gttcccaaaa ccaaagggga
     3301 tgcgctgggc tacgtttgac aggaagcggg cagagttatc agttatggag atggcttact
     3361 ggcaggcatt cagcccggta gtggacagaa tcacgggcgt tattgagcgt aaagcaggaa
     3421 aagcagccag aggcattggc ctgactctat aaaagcaaaa cgcccgcacg gggcgtcaga
     3481 gaacgggata gacttctgcg acaatttgca ttcgccgcca atatagcaag ccgtccacat
     3541 caagcaataa actatcaccg ggagggggag gccaaatctc tgcaatcctg agccttaagg
     3601 actgcccgcc ccatcgtttt ttcatacccg cgaaaaatga aatttaatcc gggcgtgttt
     3661 catccttcca gagggtatga cgtatgacac cacgcgaaat agccttattg accatcgcca
     3721 aacttgagca tggaggccac cagcttacac aggcagatca acgggagata gaacgctcag
     3781 ttaatgccga tatagcccgg cgcgacaggt tccgcgaaat gatgcgagca cctgcctacc
     3841 agtggaagaa gccagcgccg cgcaggtaga tacgggatta ttccgcatgt aacttatgcg
     3901 tattaagcaa atttccttta ttcaccttta cagaattgtg agtacgtcag tatcgttatc
     3961 cttccgattc gcatttattc aggatttgca tgtaccagct aaagataacc atccgagaca
     4021 gcaaaccacc tatatggcgg cgtgtgctgg ttccagagca aatccccttt agtgaacttc
     4081 acgcggtgat ccagttggct tttggctgga atgacgaaca cctgtatatg ttcgagaaag
     4141 ggcgtaaagg tgatcccggt agcgagtatc gcgtatgggg tgaggatgaa agcatgggta
     4201 atgcggcaat cacaccacta tgggcagcgc tccagaatga tggtgacaag ctggtttaca
     4261 cgtatgactt tggcgactgg tgggattgcg tcattgtgct ggagaagcaa acacacgata
     4321 cgagcaatca gcccattagc tgcctgcgtg ggaaaggcac caccccggtg gaaaactcag
     4381 gcggcttgca tggttataat gaattgctat tacaggccag agagtctgat aatcctgagc
     4441 aggccgagat ccacaatttc ctgatgctgg atattgagcg ccgggtttac gacctgagca
     4501 gcatcaacga cagattgcag actcttaact gacccgcata tattcgcctg atattgcgtt
     4561 atcagcgccg ccagtatcgc gcataacagc gcactgaccg ggcaaaccgt agcgcacgac
     4621 cgttgcgtta gtctcaccgc cgcgccgtat ctggcgcgat tcatacactg tcacccgctc
     4681 aagcagtcca ccagcaacca tgctttccag tgtgcgccgg gttgattcga gctggtgacg
     4741 cttatcgaac gacaccatgc catgaagcag gtaggccaca cccgacacat cgaacggcgg
     4801 cgcaccaatc tcaccagtca cccattcgag gttatccggt tcaaagtagc taagtatctc
     4861 ttttttgcgg ctggtcattc tcatagctgg caggttcctt attgtgggat agcactatca
     4921 tacaataagg cgcaaaagat aagaggccag aacagcaagc atgagagcat aaaacgagca
     4981 gccaaagaca gcgcctcaaa ataccgtgta cacaagggtg tacctactga gaaaacgcat
     5041 tacaagaaac acagcaacca cgggcgttta aagccactac tcaataaaca gattcaactc
     5101 gtaacgccaa ttcttacttt tcagccttca caaatgccgc ccactcaaac agagcggcat
     5161 ttttcttccc cgcaacttca attctgtttt gcgtgcctgc tccagatttt gcgatgtttt
     5221 tttgcccagc acactgagaa cgtgagatac tcacaactgt atataaatac agttacagat
     5281 ttactttctt tgcaatcgat atcacatgga gtgggcaatg aacgaacatc atcaaccttt
     5341 tgaagaaata cggcattatg gcactgaagg gcaagaattt tggtcagcta gagaattggc
     5401 accgttactg gattatcggg actggcgcaa ttttcaaaag gtactggccc gcgcgactca
     5461 agcgtgcgaa gccagcaatc aagctgcatc tgaccatttc gttgaaacca ccaaaatggt
     5521 tgtattaggt tcaggggctc aacgagaact tgaagatgtt catctctccc gctatgcctg
     5581 ttacctggta gtacaaaacg gcgaccctgc gaaaccggtt attgcggcag ggcaaactta
     5641 ttttgctatc cagacccgac ggcaggagct ggctgacgat gaagctttca ggcaacttcg
     5701 tgaagacgaa aagcgtcttt ttctgagaaa tgaattgaaa gaacataaca aacaattggt
     5761 tgaggcagcg cagcaagctg gggtagcgac agctatcgat ttcgccatat ttcagaatca
     5821 tggttaccgg gggctgtatg gtggattaga tcagaaagct attcatcagc ggaaggggct
     5881 gaaaaagaat cagaatatcc tggatcatat gggttcaaca gaactggcgg ctaatctctt
     5941 tcgagctacc caaacagaag aaaaactcaa gcgggatggc gtgaattcaa aacagcaagc
     6001 aaacaccaca cactttgacg tgggtcgcaa ggtgaggcaa accattcagg aacttggcgg
     6061 aaccatgcct gaggagttgc cgaccccgca ggtcagcatc aagcagttgg aaaacagtgt
     6121 aaaaattaca gagaagaaat agcagaaact aaaaactctt tttcttaatt gagatacccg
     6181 atgcgtaagc caaagtccag gtgcagtgaa cacaatggct aaacattgca cctttctttt
     6241 ccacccctca attttaacct attttttctt atgcattttc tcagacaaga actcagaata
     6301 atgcatctac gctacagaaa atagcgattt cacataacta caagttatca atttcctctt
     6361 accttaaaaa atctcaatcg tgacaatgcg cacaaatcgc taccctgcca gacagatttt
     6421 tagggagaga accatgctgt tacacatttt gtatttggtt ggcatcactg ccgaagccat
     6481 gacgggggcg ctggcggccg gacgacggcg catggataca tttggcgtaa ttattattgc
     6541 gaccgccacc gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc actatccgct
     6601 cggctgggtc aaacacccgg aatatgtgat tatcgtcgct accgccgcag tactcaccac
     6661 tatcgttgct cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg atgcgctcgg
     6721 actggtggtc ttttctatca tcggcgcaca ggttgcactg gatatggggc acggcccaat
     6781 tattgccgtt gtcgcggcgg taaccaccgg cgtgtttggc ggcgttttgc gcgatatgtt
     6841 ctgtaaacgc atcccactgg tattccagaa agagttgtat gctggtgtct catttgcctc
     6901 cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac catgatgtgg tgattatctc
     6961 caccctggta ttcggctttt ttgcccgctt actggcgcta cgtcttaagc tgggattacc
     7021 ggtcttttac tacagccacg aaggtcacta aggttcaaaa cctgtgatct gctgggcagc
     7081 cagccaactg cccagcttct tgatttgcgc attttccttc cattcaataa cctgtctggc
     7141 gcgcccagat ccagtccccg gcagctgctg tcagaactgc tccgtgctaa ataaaagttg
     7201 cgactaggac cgttcatcac tggcattaag cgccgcccgg gttagcggta ttcccattgc
     7261 catcacccag cgagtaaaag gctgcttacg agccagatta aactgatgcc ataactgcgc
     7321 acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca
     7381 agaaaagata tgctcaaagc gatgagtctg atgcagcgcg cgccaaccgg cctcaccaat
     7441 gccatccagc ccaagaacct gttttgaccc ca
//
LOCUS       DADLTD010000190         7435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLTD010000190 DADLTD010000000
VERSION     DADLTD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.192"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            167..274
                     /locus_tag="JN701_003381"
     CDS             167..274
                     /locus_tag="JN701_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365716.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(327..1085)
                     /locus_tag="JN701_003382"
     CDS             complement(327..1085)
                     /locus_tag="JN701_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY7365717.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1372..2301
                     /gene="pfkB"
                     /locus_tag="JN701_003383"
     CDS             1372..2301
                     /gene="pfkB"
                     /locus_tag="JN701_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY7365718.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2402..2692
                     /gene="ghoS"
                     /locus_tag="JN701_003384"
     CDS             2402..2692
                     /gene="ghoS"
                     /locus_tag="JN701_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7365719.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2798..3658
                     /locus_tag="JN701_003385"
     CDS             2798..3658
                     /locus_tag="JN701_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY7365720.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3699..4235)
                     /locus_tag="JN701_003386"
     CDS             complement(3699..4235)
                     /locus_tag="JN701_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365721.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4382..5050
                     /gene="hxpB"
                     /locus_tag="JN701_003387"
     CDS             4382..5050
                     /gene="hxpB"
                     /locus_tag="JN701_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY7365722.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5213..5803
                     /locus_tag="JN701_003388"
     CDS             5213..5803
                     /locus_tag="JN701_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7365723.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKHQVEY"
     gene            5936..7327
                     /locus_tag="JN701_003389"
     CDS             5936..7327
                     /locus_tag="JN701_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAY7365724.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPMLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
BASE COUNT         1867 a         1810 c         1836 g         1922 t
ORIGIN      
        1 attttcgata tatatgttga tgcataatat tattttttca taaaacgcag aaacagtgac
       61 caggtgtaca acgccaccca acggctgtaa taacaaaaaa tcccttgatg cccgtccctt
      121 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
      181 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
      241 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
      301 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
      361 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtaac gttgtaagtc
      421 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
      481 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
      541 catgcataag ccccggaggc atagcccagc ggctgagttt cactggcatt gtcggtatat
      601 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
      661 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
      721 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
      781 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
      841 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
      901 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
      961 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1021 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     1081 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     1141 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     1201 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     1261 atatatattt atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt
     1321 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     1381 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     1441 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     1501 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     1561 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     1621 gaagccaaag actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag
     1681 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     1741 caagttctgg aaattgaatc cggggccatc ctagtcataa gcggaagcct gccgccaggt
     1801 gtgaagctgg aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc
     1861 atcgtcgaca gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg
     1921 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac
     1981 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     2041 tcccttggtc cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca
     2101 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     2161 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     2221 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     2281 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     2341 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     2401 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     2461 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     2521 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     2581 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     2641 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     2701 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     2761 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     2821 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     2881 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     2941 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3001 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     3061 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     3121 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     3181 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     3241 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     3301 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     3361 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     3421 tttcgaccct gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca
     3481 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     3541 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caaggttatt
     3601 tggcggtcag catttggtta ttgctcagca gtcactggat agattattag cagcatgata
     3661 tgggttgaag ataatggccg ctctgtgcgg ccttttgatt aaataaaccc aagcagagaa
     3721 aagaacacat aacctgcagc aatacaaata accggcagga tatacagtga gaaaaactgc
     3781 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     3841 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     3901 gcctggcgac tcattcgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     3961 aaaatcaccc agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac
     4021 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag
     4081 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     4141 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     4201 aaaacagcaa tgtgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     4261 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     4321 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     4381 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     4441 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat
     4501 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     4561 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     4621 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     4681 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     4741 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     4801 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     4861 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     4921 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     4981 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     5041 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     5101 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     5161 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     5221 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     5281 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     5341 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     5401 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     5461 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     5521 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     5581 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctag ttccccaaaa
     5641 gggcaaccaa ctggaacgtt ttatctgtat ggcattattt gtctggtcgg tatggatgcc
     5701 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaataatca ataccttgca
     5761 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaagaca aaaatcacct
     5821 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     5881 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     5941 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6001 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     6061 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     6121 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     6181 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     6241 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     6301 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     6361 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag
     6421 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     6481 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     6541 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct
     6601 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     6661 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     6721 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct
     6781 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggccta tgctgacgtt
     6841 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     6901 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     6961 tcagaacggc tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt
     7021 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     7081 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     7141 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     7201 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     7261 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     7321 ccgttaattc ttatgctggc aatggtccgt tttgtatagg ggccgttgct ttacttttta
     7381 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaa
//
LOCUS       DADLTD010000191         7408 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLTD010000191 DADLTD010000000
VERSION     DADLTD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.193"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..1092
                     /locus_tag="JN701_003390"
                     /pseudo
     CDS             1..1092
                     /locus_tag="JN701_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1144..1458
                     /locus_tag="JN701_003391"
                     /pseudo
     CDS             1144..1458
                     /locus_tag="JN701_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1469..2542
                     /locus_tag="JN701_003392"
     CDS             1469..2542
                     /locus_tag="JN701_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7365725.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2790..3830
                     /gene="pstS"
                     /locus_tag="JN701_003393"
     CDS             2790..3830
                     /gene="pstS"
                     /locus_tag="JN701_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY7365726.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3917..4876
                     /gene="pstC"
                     /locus_tag="JN701_003394"
     CDS             3917..4876
                     /gene="pstC"
                     /locus_tag="JN701_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY7365727.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDVPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4876..5766
                     /gene="pstA"
                     /locus_tag="JN701_003395"
     CDS             4876..5766
                     /gene="pstA"
                     /locus_tag="JN701_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY7365728.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5857..6630
                     /gene="pstB"
                     /locus_tag="JN701_003396"
     CDS             5857..6630
                     /gene="pstB"
                     /locus_tag="JN701_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY7365729.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6645..7370
                     /gene="phoU"
                     /locus_tag="JN701_003397"
     CDS             6645..7370
                     /gene="phoU"
                     /locus_tag="JN701_003397"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY7365730.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1864 a         1717 c         1932 g         1895 t
ORIGIN      
        1 agatgttgat gtcagcgtgg gtgaattgcg caatgaagac ggatttacac cggacgtcct
       61 tcaggcgcaa atacttcatg gtttttccca cgggatcact ttatatggag gtatgcaggc
      121 tgctgaaaat tatggttctg cagctctggg tgtcggtaaa gatcttggcg ctttgggcgc
      181 aatttctttc gatgtgacac atgctcgtgc gaattttagc catgatgata cagaaacggg
      241 tcagtcatat cgctttctct attcaaaacg atttgacgac acagacacta gcttgcgcct
      301 ggttggctat cgttactcca ccgagggcta ctataccctc aatgagtggg catcgcggcg
      361 caacagccct gaagactttt gggaaacagg taaccgacgt agtcgcgtgg agggaacgct
      421 aacgcagtcg ttggggagag attatggcaa tttatacctg acattaagcc ggcaacaata
      481 ctggcatacc gatgatgtcg aacgattaat gcaatttggc tacagcagta gctggaagcg
      541 tctctcgtgg aacgtctcct ggagttattc caatactgcc agacagggga cggggaacaa
      601 ccatgccagt aataacacca gtgagcagat ctacatgctc tctttatctg ttcctttatc
      661 gggctggtgg ggtaatagtt acgccaccta ttctgtttcg caaaacgata attccggtag
      721 ctcacatcaa gcggtacggc gctggaaaga aataaccttt catggaattt aatgcagtcc
      781 tataacagtc atgatgatga ggttggcggt aatatgtccc tgacctatga tggctcttat
      841 ggcacggtga acggcagcta taactacagc caaaattccc agaggctgaa ttatggtatc
      901 agagggggaa ttctggcaca cagcgaaggg gtaacgttaa gtcaggagtt aggtgaaact
      961 attgctcttg ttaaagcacc tggggccgcc gggttagaaa tagataatat gcgcggtgct
     1021 gcgacggact ggcgcggcta tacggtcaag acacagctaa acccttatga tgaaaatcgg
     1081 gtagcaatca gcgataacta tttctcgaag tcgaatatag aacttgataa taccgtcgtt
     1141 acgatggttc ccacgcgtgg tgcagtggtt aaagcggagt ttgtgactca tgtgggttat
     1201 cgcgttctct tcagagtgtt aaatgcaaat ggtaaaccgg taccttttgg agccattgct
     1261 gcgatacaag atgcaagttt ggcagattca ggaattgtcg gtgaccgtgg cgaactttat
     1321 ctttctggtc taccagaaaa aggataggtt acgttatcct ggggagaaaa cgcctcaaca
     1381 aaatgcatct tcaattattc actttcgaca ccagaaagtg agagcggatt aattgaacag
     1441 ggtgtgacat gtcattaaaa ggatttttat gaacaagtat atcaaacagt ggtgctttgc
     1501 tgtgtttatg ctctcgttaa gtagtgtagc acttgcagct cctaaaggta tctgtacccc
     1561 tgataatggc gtatttcaca gtacgcttga tttctcggga tatcttatta ccgcgaacga
     1621 aaataaggtt ggaacgacat ttaacaccac tgtaactaat ggatcctcat atcctggacg
     1681 ctgtcattgt gatactggta acgtaggaga attcccctat atctattata cgtcaaaaat
     1741 aaaccaggca ttaacttatg ctggggttca ctctaatatt aattattatg atcttaatcc
     1801 gaaccttgat gttgggattg ctatagatat tcttggggtt gggtatgtga atgcaccttt
     1861 tgaatatcat gctaacaacc cgtctggtaa tacaaagtac aattgcaacc gcattgaacc
     1921 tttaagtata tctagtgggg ctaaagcgat agtatatttt tatatcaaga agacatttgc
     1981 aggaaaattg attattcctg aaacaaagat agtgacattg tatggaacaa ttagccgtga
     2041 caccccggtg gattactcac agccgatggc tgatgtttat attcggggcg atattacagc
     2101 tccgcaaagt tgcgaaataa ataatttaca gccagtttat tttgatttta aagaaatacc
     2161 agctgcagat ttttcatctg ttgttggaag tgcggtaata acacataaaa ttactaaaac
     2221 ggtcactatt gagtgtgaaa acctggggat actgaatacc gatgatatca gtacgtcttt
     2281 ttatgctacc gaacccaata cggacaattc aatggtcgta acttcaaact ctaacgtggg
     2341 gataaaaatt tacgacaaaa ataataagga aatcaaagtg aacggtggtg agttgccaac
     2401 agacatgggt aaatcaacag tgtatggtga aaaatcaggt agtgtaactt tttcagctgc
     2461 tcctgcgagc cttactggag ctcgcccagc cccggggcaa tttactgcaa cagcgacgat
     2521 aactgttgag attgtgcgct aataggtctg acaacgattg ttcatcacat cgttgtcatc
     2581 tcacccattt ctttatcact tttaaatgca tcatgacaaa aagatgacat gtgggatttt
     2641 catcttcaca tttaaaatca gtaagttatt tggtaattcc tttctgtcat aaaaatgtca
     2701 tattccttac atataactgt cacctgtttg tcctattttg cttctcgtag ccaacaaaca
     2761 atgctttatg aatcctccca ggagacatta tgaaagttat gcgtaccacc gtcgcaactg
     2821 ttgtcgccgc gaccttatcg atgagcgctt tctctgtgtt tgcagaagca agcctgacag
     2881 gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg ggctgacact taccagaaag
     2941 aaaccggtaa taaagttaac taccagggta tcggttcttc cggtggcgta aaacagatta
     3001 tcgctaatac cgttgacttt ggtgcctctg acgcgccgct gtctgacgaa aaactggctc
     3061 aggaaggtct gttccagttc ccgaccgtga ttggcggcgt ggtgctggcg gttaacattc
     3121 cagggctgaa gtctggcgaa ctggtgctgg atggtaaaac cctcggcgac atctacctgg
     3181 gcaaaatcaa gaagtgggat gatgaagcca tcgccaaact gaatccgggt ctgaaactgc
     3241 cttcacaaaa cattgctgta gtacgccgcg cagatggctc cgggacttcc ttcgtcttca
     3301 ccagctacct ggcgaaagtg aacgaagagt ggaaaaacaa cgttggtact ggctctaccg
     3361 taaaatggcc gatcggtctg ggcggtaaag gtaacgacgg tatcgccgcg ttcgttcagc
     3421 gtctgccggg tgcaattggt tatgttgaat atgcttacgc gaagcagaac aacctggcgt
     3481 acaccaaact gatctccgct gatggtaaac cggttagtcc gaccgaagaa aacttcgcta
     3541 atgcagcaaa aggtgcagac tggagcaaaa ccttcgctca ggatctgacc aaccagaaag
     3601 gcgaagatgc atggcctatt acctctacca cgttcattct gatccacaaa gatcagaaga
     3661 aaccagaaca aggcacagaa gtgctaaaat tcttcgactg ggcgtacaaa accggggcta
     3721 aacaggcgaa cgacctggat tacgccagcc tgccggatag tgtagttgaa caggttcgcg
     3781 ctgcgtggaa gaccaatatt aaagacagta gcggtaagcc gctgtactaa taaaactcca
     3841 ggccgggtac ggtgttttac gccgcatccg gcattacaaa atgactttgt aaacgcgttt
     3901 aactgaagag taacttatgg ctgcaaccaa gcctgctttt aacccaccgg gtaaaaaggg
     3961 cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg attgtgctat tgatgttggg
     4021 tggcattatt gtctctctga tcatctcctc ctggccgagc attcagaaat ttggtctggc
     4081 tttcctatgg accaaagagt gggatgtacc gaacgatatc tacggggcgc tggtgccgat
     4141 ctacggtacg ttggtgactt cgtttatcgc gctgctgatc gccgtcccgg tgagtttcgg
     4201 tatcgccctg ttcctgactg agcttgcgcc tggctggctg aaacgcccgc tgggtatcgc
     4261 cattgagctg ctggcagcca ttccaagtat cgtttacggc atgtggggcc tgtttatctt
     4321 tgcgccgctg ttcgccgttt actttcagga gccggtcggc aatatcatgt cgaatatccc
     4381 gattgttggc gcgctgttct ctggccccgc atttggtatc ggtatcctcg cggcaggcgt
     4441 gatcctcgcc atcatgatta ttccgtacat tgcggcggta atgcgtgatg tgttcgaaca
     4501 aaccccggtg atgatgaaag agtcggccta cggtattggc tgcaccacct gggaagttat
     4561 ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc ggcggcatca tgctggggct
     4621 gggccgcgcg ctcggtgaaa ccatggcggt gacctttatc atcggtaaca cctaccagct
     4681 cgacagcgcc tcgctgtaca tgccgggcaa tagtatcacc tctgcgttgg cgaacgaatt
     4741 tgcggaagcg gaatccggtc tgcacgttgc cgcactgatg gaactgggcc tgatcctgtt
     4801 tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg attatgcgcc tggctaagaa
     4861 tgagggggca cgctaatggc tatggttgaa atccaaacca ctgcggcgct ggctgaatct
     4921 cgccgcaaaa tgcaggcgcg tcgccgcctc aaaaaccgta ttgcgctgac gctctcgatg
     4981 gcgacgatgg ccttcggcct gttctggctg atctggattt taatgtccac catcactcgc
     5041 ggtatcgacg gtatgtcgct ggcgctgttc actgaaatga cgccgccgcc caatacggaa
     5101 ggtggtggtc tggcgaacgc cctggcgggt agcgggctgt taattttgtg ggccacggta
     5161 ttcggtacgc cgctgggcat tatggcgggg atttatctgg cggaatatgg tcgtaaatcc
     5221 tggctggcag aagtgattcg cttcattaac gacattctgc tctccgcgcc gtcgattgtg
     5281 gttggtctgt ttgtttacac catcgtggtg gcgcagatgg agcacttctc tggctgggcg
     5341 ggcgtgattg ccctggcgct gttgcaggtg ccgattgtta tccgcaccac cgagaacatg
     5401 ctgaaactgg tgccggacag cctgcgtgaa gcggcttatg cgctgggtac accgaagtgg
     5461 aagatgatct ctgcgattac gctgaaagcg tcggtgtccg ggatcatgac cggtatcctg
     5521 ctggcgattg cccgtattgc tggtgaaacc gcgccgctgc tgtttaccgc gctctccaac
     5581 cagttctgga gcacggacat gatgcagccg atcgccaacc tgccggtgac gatctttaag
     5641 tttgcgatga gcccgtttgc ggaatggcaa caactggcct gggccggggt attgattatt
     5701 accctgtgcg tactgctgct gaacattcta gcgcgcgttg tttttgcgaa gaataaacac
     5761 ggttaacact ttgccggatg cggcgtgaac gccttatccg gcctacggta agcctgatta
     5821 gcgaagtgca tcaggcacga tgaggaaaag attgcaatga gtatggttga aactgccccg
     5881 agtaaaattc aggttcgtaa tttgaacttc tactacggca aattccatgc cctgaaaaac
     5941 atcaacctgg atatcgctaa aaaccaggtt acggcgttta tcgggccgtc cggctgcggt
     6001 aaatcgacgc tgctgcgtac cttcaacaaa atgtttgaac tgtacccgga gcagcgtgcg
     6061 gaaggtgaaa ttctgcttga tggcgacaac atcctgacca actctcagga tatcgcactg
     6121 ctgcgtgcga aagtgggcat ggtgttccag aaaccgacgc cgtttccgat gtccatctac
     6181 gacaacatcg cttttggcgt tcgtctgttt gagaagctct cccgtgccga catggacgag
     6241 cgcgtgcagt gggcattgac caaagccgca ttgtggaacg aaaccaaaga taaattgcac
     6301 cagagcggtt actctctctc tggtggtcag caacagcgtc tgtgtattgc gcgtggtatc
     6361 gccattcgcc cggaagtgct gctgctcgac gaaccgtgtt cggcgctcga ccctatctct
     6421 accgggcgta ttgaagagct gatcaccgaa ctgaagcagg attacaccgt ggtgatcgtc
     6481 acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca cggcgtttat gtacctgggc
     6541 gaattgattg agttcagcaa cacggacgat ctgttcacca agccagcgaa gaaacaaaca
     6601 gaagactaca tcaccggtcg ttacggttga ttcaggagtg cgttatggac agtctcaatc
     6661 ttaataaaca tatttccggc cagttcaacg ccgaactgga aagtatccgc acgcaggtga
     6721 tgaccatggg cggcatggtg gagcagcagc tttctgatgc aatcaccgcg atgcataacc
     6781 aggacagcga tctggcgaag cgcgtgatcg aaggcgacaa gaacgtcaac atgatggaag
     6841 tggcgatcga tgaagcctgc gtgcgcatta tcgccaaacg tcagccgacg gcgagcgacc
     6901 tgcgactggt tatggtgatc agtaaaacca ttgccgagct ggagcgtatt ggcgacgtgg
     6961 cggacaaaat ctgccgtact gcgctggaga aattctccca gcagcatcag ccgttgctgg
     7021 taagtctgga gtcgctgggc cgtcatacca tccagatgct gcacgacgtg ctggacgcgt
     7081 tcgcgcggat ggacattgac gaagcggtac gtatttatcg tgaagataaa aaagtcgatc
     7141 aggaatacga aggtattgtt cgtcaactga tgacctacat gatggaagat tcgcgtacca
     7201 ttccgagcgt acttactgcg ctgttctgcg cgcgttctat cgaacgtatt ggcgaccgct
     7261 gccagaatat ttgtgagttt atcttctact acgtgaaggg gcaggatttc cgtcatgtag
     7321 gcggtgatga gctggataaa ctgctggcgg gaaaagatag cgacaaataa ttcaccagac
     7381 aaatcccaat aacttaatta ttgggatt
//
LOCUS       DADLTD010000192         7405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLTD010000192 DADLTD010000000
VERSION     DADLTD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.194"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..882)
                     /gene="adiC"
                     /locus_tag="JN701_003398"
     CDS             complement(<1..882)
                     /gene="adiC"
                     /locus_tag="JN701_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY7365731.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWT"
     gene            complement(1019..1780)
                     /locus_tag="JN701_003399"
     CDS             complement(1019..1780)
                     /locus_tag="JN701_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7365732.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFHVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2105..4372)
                     /gene="adiA"
                     /locus_tag="JN701_003400"
     CDS             complement(2105..4372)
                     /gene="adiA"
                     /locus_tag="JN701_003400"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY7365733.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(4571..5479)
                     /gene="melR"
                     /locus_tag="JN701_003401"
     CDS             complement(4571..5479)
                     /gene="melR"
                     /locus_tag="JN701_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY7365734.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            5762..7117
                     /gene="melA"
                     /locus_tag="JN701_003402"
     CDS             5762..7117
                     /gene="melA"
                     /locus_tag="JN701_003402"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY7365735.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7220..>7405
                     /locus_tag="JN701_003403"
     CDS             7220..>7405
                     /locus_tag="JN701_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY7365736.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIM"
BASE COUNT         1851 a         1755 c         1924 g         1875 t
ORIGIN      
        1 cgtccagccg cccagtgaac ctaagcaacc cgcagctgcg cagaaggaaa caatggcccc
       61 gggggtgtca cccaacgcca tccgtgcggc atcaccgaat ggcgaagcag aaacgcgcag
      121 tgcggcatta gggatcatcc ccataatcgc ggtggtagaa agtacatagc aaacggcggc
      181 aatcaatacc ccaccaatgg tggcgatagg gacattgcgt ttcgggtttt tcaccacacc
      241 tgcggcaacg gaggcacttt ccacaccgat gaacgaccac agcgtaacgt taagggtact
      301 ttgaattgca ccgaaggtac ccaggccgct gacgttccat gccgccatat aggtttcacc
      361 acggaaccag aaccagccaa atacggcaat cccgacgatg gggatcagcg ccagcacggt
      421 ggcaactgcc tgcacacggg tgatcatttt cggaccaaca atgttcagca ggacgaagat
      481 ccacagcacc acgacgcagg tgatggttaa taccaatgga tctttcagaa tcgggaagaa
      541 gtaacttaaa tatcctacgc caatgaccac catggcgata ttgccgatcc agcaggccag
      601 ccagtagagg acgttggttt gataaccgag aaacgggcca aagcagcggc gggcgtaagc
      661 gtaagaacca ccaggacttg ggtcgaggaa cgacattttg gcgtatacca tcgagagccc
      721 cagtgcaccg ataatcgtca ccaaccatcc ataaatggca atcccgccag tggaggccag
      781 gtttgcaggt aacagaaaaa cacctgaccc cataatattc cccgacacca tcagggtgac
      841 ggggattaag cccactttgt gagcatcagc atccgaagac ataattaaac tcctgcgaag
      901 gcgagcttcg tgacaataaa ttacgtcata tcatacgcct gcattcgata gttgaattat
      961 tatcgaccgg gttatcgctg atgccgttat taatatagct cttcgttaag gttaaaaatc
     1021 aggcgacagt gcgttctctg tgctgactaa cataatgcag cggcgtcata ccgtagaagt
     1081 ctttaaagac agaaataaag tacgacgtac tgttgtagcc gcaggactgt gatacctgag
     1141 agatattttt accgtccatc attaattcat ttacggcata acgcatgcgg caggtggtga
     1201 ttatttggct ataactggtg ttttcgcttt tcagcttttt ctttaacaga cttgggctaa
     1261 gacataaaca actggctacc atactaagat tccagtcttt gtgaatatcg ctttcaataa
     1321 tttgataaac gctgtcactt acacaattac gtaacatata catcattaat gaaaggaatt
     1381 ttttactgtc gagaaaacgg gatagcaccg tgaataataa tgcgcgtgtt cgttcctttt
     1441 ctgactcagt ttctgcgggc atcatgctgt gttgcgcggc aacgtgaaag acgtctggcg
     1501 tcaggcatgg cagggtgagt atgggcgtag cgctacgttg ccatactggt atttgcgaga
     1561 gatctttatt cagaaaccgg aggtaatctt tgatgatgtc gtgactaata tgagcaacca
     1621 aagtgttgtt aagagaggag acgtcgataa tattattttc acaatttaat aacgccatat
     1681 ggttagcttt aaggctaata ggttctttcc cattcaccct tatccagaca tctttttcag
     1741 tcaataaaac aatacaaggt tggtcgctgc aaatcctcat acatgtactc ctgagtgcga
     1801 ataaaagacc agttctcgct aaagcaaagc gataccggga aatggttata taattgtatg
     1861 acgaatataa acatgcataa aaataacgag ttttactaat attacaaatt tttatagtaa
     1921 tttctcatgt acatattttt ttacagggta aataacaggc aaaaaaaagc gcggggtggg
     1981 gccgcgcaaa aagtatgaca tttttgtttg cagcaatttt tatgaaaacg ttgccatact
     2041 caaaaggcct aaggccaggc ggcgtaatgt tatttaaaca attacgcctt cagcggaata
     2101 gtggttacgc tttcacacac atagcgtggt aaataccgtc aataatttca gtcccttcag
     2161 tttcgtgttc aaatccaggg aaatggtggt cccaggattg cagcgagcgt aaataactta
     2221 cttgcggact gtttttatcg ccgaagtttt caccagacag cagcatcggg attcctggcg
     2281 gatatgggat aactgagttt gccgcgatgc gtcctggcag attttcaatg gataccagtt
     2341 cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg ggtgatttcc gccaccggca
     2401 ggccggaata ggcttcgttc aaccgtgcgc cagggttgtt ttctttcagc caggcaaaca
     2461 tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt gtcaggatat tgttcaacaa
     2521 gttccggcat cacctgcgcc agcggtgtgt tggcgtcata gtggcgtttg aaggagcaaa
     2581 gggtgttaac cagagtcccc catttcccac gggttacgcc catagagaac aggaacataa
     2641 tttggaagtc agtggtgcgg gtaggcacaa tgccgtggcg accaagccag gcagtgacca
     2701 gcgccgccgg aacaccggtt tcttccagtt caccatcttc acccattccc ggagcaagga
     2761 tgctgacttt aatcgggtcg agcatactcc agttatccgg aatatctttg aagccgtgcc
     2821 agctttcgcc cggatgcatt acccagtagt cctgaacggt ggtcagcagt ttggttggtg
     2881 cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt gacgacttct ttgttccacg
     2941 gtttgaagaa ccagctaccg tcagcggtga actctttata cagccgcgcc atcgcctgac
     3001 ggaaatcaac cgcttcgtcg atcacttcct gcgtcagtga caggccgctg ttgccgtcca
     3061 tcatcgacac cgccacgtcg ttggatgcgc agatggcata cagcggggag gtggtggcat
     3121 gcatcatgta ggcctggttg aagcgggaga agttaatcgc cccacgacct tcacgtacat
     3181 gaatataaga agcctgtgaa agcgcattca gcagtttgtg ggtggagtgg gtggcgaaaa
     3241 cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc atagtgatcg gcatagatcg
     3301 ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg cagacgatcg gaggtttttt
     3361 ccagcagatc ctgcgcttct ttagcgttat aacacacgcc gtcataggtg cagttggtca
     3421 ccacgcagta agacggtttt tgcccggctt tgtctttagt cagcgggctt tcactgattt
     3481 tcttctgcaa ggtttcaggt tgcatttcct gcggatagat tggcccgata atgccgtagc
     3541 ggttgcggct tggcaccatg tagaccggtt tcgcgcctgt cagcatcaag ccttgttcga
     3601 tggatttatg gcagttacgg tcaacgacca cgacatcgtt atcggtcatg caagcctgca
     3661 tgatggtgcg gttagagccg gaagtaccga cgactaccga ccaggagcga tcggcaccaa
     3721 atacgcgtgc ggcatatttt tcgctttcgc caaatgcgcc agtatggtca agcaaagaac
     3781 cgagggaagt tcgttcgatg cccatatcgg tgcggaacag attttctcca tagtagtcat
     3841 ggtagaaacg tccggcgggt gttttggtaa aaccaacgcc gccctggtgg cctggcgctg
     3901 cccaggaata ttcatggatg tcgctatact tcatcagcgc gctgaacagt ggcggcaaca
     3961 gctgctggcg gtagcgggtc atcgcggcaa cggcgcgtcc ggcgataaag tcggtggtat
     4021 cttccagaat ccaggcgaat tcatcgacaa gctccagcag gtcgcgatcc attgcggcga
     4081 gggctttttc ccgatcgccc aacaggaaga ccggcacgtt ttgttggcgc tcatgaagct
     4141 taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg ttccatttga tagctgaaca
     4201 tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa accatcatca aaggaggtgg
     4261 atttaatcac ggtaacattt tgctggctta aagcatctgc cagacgctca acggcgttac
     4321 cgacccaggt gtcttgatgg agaaactcgc tttcaacaat taatactttc atcattgctt
     4381 acccggttat gaaggaatct tcgttgcggg caagtatctt ccggcctttt cgggctgtaa
     4441 agcgcgtgaa aaacaaacat ttttgaaata tttattttta taaacaatgc gttatgtgat
     4501 tattttaaat tattttcact gcttgttatc gacggctaaa ctattttttt ggctgatact
     4561 gatatcgtct ttagccggga aacgtctggc ggcgctgttg gctaagtttg cggtattgtt
     4621 gcggcgacat gccgacatat ttgccgaacg tgctgtaaaa acgactactt gaacgaaagc
     4681 ctgccgtcag ggcaatatcg agaatacttt tatcggtatc gctcagtaac gcgcgaacgt
     4741 ggttgatgcg catcgcggta atgtactgtt tcatcgtcaa ttgcatgacc cgctggaata
     4801 tccccattgc atagttggcg ttaagtttga cgtgctcagc cacatcgttg atggtcagcg
     4861 cctgatcata gttttcggca ataaagccca gcatctggct aacataaaat tgcgcatggc
     4921 gcgagacgct gtttttgtgt gtgcgcgagg ttttattgac cagaatcggt tcccagccag
     4981 agaggctaaa tcgcttgagc atcaggccaa tttcatcaat ggcgagctgg cgaatttgct
     5041 cgttcggact gtttaattcc tgctgccagc ggcgcacttc aaacgggcta agttgctgtg
     5101 tggccagtga tttgatcatc atgccgtgag tgacgtggtt aatcaggtct ttatccagcg
     5161 gccaggagag aaacagatgc atcggcagat taaaaatcgc catgctctga caggttccgg
     5221 tatctgttag ttggtgcggt gtacaggccc agaacagcgt gatatgaccc tgattgatat
     5281 tcactttttc attgttaatc aggtattcca catcgccatc gaaaggcaca ttcacttcga
     5341 cctgaccatg ccagtggctg gtgggcatga tatgcggtgc gcgaaactca atctccatcc
     5401 gctggtattc cgaatacagc gacagcgggc tgcgggtctg tttttcgtcg ctgctgcaca
     5461 taaacgtatc tgtattcatg gatggctctc tttcctggaa tatcagaatt atggcaggag
     5521 tgagggagga tgactgcgag tgggagcacg gttttcaccc tcttcccaga gaggcgaggg
     5581 gagcgtccga gcatcatgag gccgaaaact ctgcttttca ggtaacttat taccataaac
     5641 tcagatttac tgctgcttca cgcaggatct gagtttatgg gaatgctcaa cctggaagcc
     5701 ggaggttttc tgcagattcg cctgccatga tgaagttatt caagcaagcc aggagatctg
     5761 catgatgact gcacccaaaa ttacatttat cggcgctggt tcgacgattt tcgttaaaaa
     5821 tattcttggt gatgtgttcc atcgagaggc gctgaaaacg gcgcatattg ccctgatgga
     5881 cattgatccc acccgcctgg aagagtcgca tattgtggtg cgtaagctga tggattcagc
     5941 aggggccagc ggcaaaatca cctgccacac ccaacagaaa gaagccttac aggatgccga
     6001 ttttgtcgtg gtggcatttc agattggcgg ttatgaacct tgcacggtga ctgatttcga
     6061 ggtctgtaag cggcatggtc tggaacaaac cattgccgat acgttggggc cgggcggtat
     6121 tatgcgcgcg ctacgtacca ttccgcatct gtggcaaatt tgcgaggaca tgacggaagt
     6181 ctgccccgat gccacattgc tcaactacgt taacccaatg gcgatgaata cctgggcgat
     6241 gtatgcccgc tatccgcata tcaaacaggt agggctgtgc cattcggtgc agggaacggc
     6301 ggaagagctg gcgcgtgacc tcaatatcga tccgtccacg ctgcgttacc gttgcgctgg
     6361 tatcaaccat atggcgtttt acctggaact ggagcgtaaa accgccgacg gcagttacgt
     6421 gaatctctac ccggaactgc tggcggctta tgaagcaggg caggcaccga agccacatat
     6481 tcatggtaat actcgctgcc agaatattgt gcgctacgaa atgttcaaaa agctgggcta
     6541 cttcgtcacg gaatcgtcag aacattttgc cgaatatacg ccgtggttta ttaagccagg
     6601 tcgtgaggat ttgattgagc gttataaagt accgctggat gagtacccga aacgctgcgt
     6661 cgagcagctg gcgaactggc ataaagagct ggaggagtat aaaaacgcct cccggattga
     6721 tattaaaccg tcacgggaat atgccagcac aatcatgaac gctatctgga ctggcgagtc
     6781 gagtgtgatt tacggcaacg tccgtaacga tggtttgatt gataacctgc cacaaggatg
     6841 ttgcgtggaa gtagcctgtc tggttgatgc taatggcatt cagccgacca aagtcggtac
     6901 gctaccttcg catctggccg ccctgatgca aaccaacatc aacgtacaga cgctgctgac
     6961 cgaagctatt cttacggaaa atcgcgaccg tgtttaccac gccgcgatga tggacccgca
     7021 tactgccgcc gtgctgggca ttgacgaaat atatgctctt gttgacgacc tgattgccgc
     7081 ccacggcgac tggctgccag gctggttgca ccgttaaaac gcgactaaac gctactgcgc
     7141 cgggggattt attccggcgc acacctctga cgataccaat aacagaaggc gggcgttggt
     7201 aacagcgacc cgatacccta tgagcatttc aatgactaca aaactcagtt atggatttgg
     7261 agcgttcggg aaggattttg cgatcggcat tgtgtatatg tacctcatgt attactacac
     7321 cgatgtcgtt gggctgtctg tgggtttggt cggtactttg tttctggtgg cgaggatctg
     7381 ggatgctatt aacgatccga ttatg
//
LOCUS       DADLTD010000193         7316 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLTD010000193 DADLTD010000000
VERSION     DADLTD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.195"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(214..1752)
                     /gene="lnt"
                     /locus_tag="JN701_003404"
     CDS             complement(214..1752)
                     /gene="lnt"
                     /locus_tag="JN701_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY7365737.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(1777..2655)
                     /gene="corC"
                     /locus_tag="JN701_003405"
     CDS             complement(1777..2655)
                     /gene="corC"
                     /locus_tag="JN701_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY7365738.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(2745..3212)
                     /gene="ybeY"
                     /locus_tag="JN701_003406"
     CDS             complement(2745..3212)
                     /gene="ybeY"
                     /locus_tag="JN701_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY7365739.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(3209..4288)
                     /locus_tag="JN701_003407"
     CDS             complement(3209..4288)
                     /locus_tag="JN701_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY7365740.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            complement(4402..5826)
                     /gene="miaB"
                     /locus_tag="JN701_003408"
     CDS             complement(4402..5826)
                     /gene="miaB"
                     /locus_tag="JN701_003408"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY7365741.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            5972..7147
                     /gene="ubiF"
                     /locus_tag="JN701_003409"
     CDS             5972..7147
                     /gene="ubiF"
                     /locus_tag="JN701_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY7365742.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         1670 a         1943 c         1988 g         1715 t
ORIGIN      
        1 ttcagcggat ggtagaaaaa tatttgtcat ggcgaagaac gggttgatcc aaatggacag
       61 gacgcgctcg cttgatccgc aggcaattat tcgtcatccc caatatggca atgtggcttg
      121 tatccctgaa ccgtaagaaa aaggggcatg ataaccatgc cccaaagctc aatgagaaag
      181 tacgttttgc tcatgcagga tacatcaggc acatcaacgt ttacgtgctc gcagactcat
      241 caacacagcg gcaaaaccaa acagtgccgt cagcacccac agtggccagt tgccggtacg
      301 tgcatacggt gtgagtccgg tggtcggtgt cacattagtg gttaacacct cgcgggtgaa
      361 ctgcgggatc atcgcctgaa tctcaccctg cgggccaatc accgccgtaa tgccgttgtt
      421 ggtgctgcgc aacagtgggc gcgccagctc cagcgcacgc attcgcgcca tctggaagtg
      481 ttgccatgga ccaatagatt taccaaacca cgcatcgttg gagatagtca gcagatagtc
      541 ggtatccggg cggaagttat cgcgcacttg ctcgccgaga atgatctcgt agcaaatagc
      601 cgcagtaagc tcaataccat ttactgacag cggcggctgg atatatggcc cacggctgaa
      661 cgacgacatc ggcagatcaa agaacggtgc taacggacgc agaatcgact ccagcgggac
      721 aaactcgcca aacggcacca gatggttttt gttatagcga tcggctgatt cgtagctgta
      781 cggtgcacct ttacccagtg tgatgatggt gttgtaggta tcgtagcggt tctgcttatt
      841 gagacgcgcg tcgacaatcc cggttaccag cgagctacct ttatcgcgca actcaccgtc
      901 cagtgctttg aggaacggtt gctgattaat ttccagatcg gttatcgccg actccggcca
      961 gataatcaac gatgatttgc ccatcagcgg agccgttgcg ttgtagtaaa tcttcagcgt
     1021 attaagaagc tgatctccat cccatttcag tgattgcgga atatcgccct gaaccatcga
     1081 aacctgaatg gttttctccg gttgcggggt aaaccattgg atgtaacgca gcgggaaggg
     1141 tagggcaaac agcacgacgg ccaccaccag cggacgccag ttgcgtttga ccaacgccag
     1201 tgccagcagg ccactaacca tcatcagcag gaagttaatg gcttccacgc ccattatcgg
     1261 tgccagccct tttaacgggc catcaatctg gctatagccg aactgtaacc acgggaagcc
     1321 tgtcagtacc caaccgcgca gaaactcggt cacttgccag agagcagggg cggcaatcgc
     1381 cacgcgcagc caggtggttt tcggccacag acgcgacagc acgccagcaa acagtccggt
     1441 atacagcgac aaatacgccg ccagcagcac caccaggaag atgttaaccg ggccaggcat
     1501 tccgccaaag gtcgcgatgc tgacatagac ccagttaata ccgctgccaa agaggccaaa
     1561 tccccagcaa aagccaatag cggcagactg gagtggacgg cggttaaagg tcaacgcctg
     1621 aagccccatc aacgaaataa tcgccgcagg ccagacgtcg taaggagaga aggccagcgt
     1681 tccgcaggca ccgaataata acgccagcag caggcgaatg cgctggcgtt caattaatga
     1741 ggcaaaagcc atgtagttat ctatccagtt tcggttttat tcatccagct tcggctgggg
     1801 tgagtcatcc gggattttga catgaacctg aataatacgt cgactgtcgg ccatcgccac
     1861 tttgaactgg taaccgtcga tgtcgatagt ttcgccacgc gccggaagat gcccaaacgc
     1921 ctgcatcacc agaccaccga tagtgtcgac ttcttcatcg ctaaagtggg tgccgaacgc
     1981 ttcgttgaag tcttcaatgg aagccagtgc gcgcacggtc caggtatgac gactcagctg
     2041 acggaagtcg atatcatctt cttcgtcata ttcgtcttca atctcaccaa caatcagttc
     2101 caggatgtct tcaatggtca ccagaccgga aaccccaccg aattcgtcaa tgacgatcgc
     2161 catgtggtaa cgctgagagc gaaactcttt cagcatccgg tctacgcgct tactttcagg
     2221 aacgacaacc gcctgacgta acactttgtc catgctgaag gcttcagcat cgctgcgcat
     2281 aaacggcagc aagtctttcg ccatcagaat cccttcaatg tgatctttgt cttcgctaat
     2341 caccgggaaa cgtgagtggg cggactcgat gatgacatca aggcattcgt ccagcgtctg
     2401 gttgcgtttc agggtaatca tctgggagcg ggggatcatg atgtcgcgaa cgcgttggtc
     2461 tgcgatgtcc atcacccctt cgagcatatc gcgcgtatct tcgtcgataa ggtcgttctg
     2521 cccggaatca cggatcagcg ccagcagttc gtcacggttt ttcggttcac cgtggaaaag
     2581 ttggctgagt aacagggaga aaaatccctt cttgttgctt atcgtgtcac tactgtgtga
     2641 attgtcgtcg ctcatggcgt cgtatgggtt ctcatgttag ttaatcaaaa cgccgtcgtt
     2701 aatcaccaac ggcggggacg tctgccagtc aaatgcctgg caaattattc tttctcggca
     2761 atgtacggat cctcatagcc cagagcaagc ataatctctg tttcgagggc ttccatttct
     2821 tctgcttcgt catcttcgat gtgatcgtaa cctaacaaat gcagactgcc gtgcaccacc
     2881 atatgcgccc agtgcgcctc cagtggtttg ccttgctcct gagcttcctt ctcaaccacc
     2941 tgacggcaga taaccagatc gcccagtagc gacatttcca tgccaggcgg cacttcaaac
     3001 gggaaggaga gcacgttggt cggcttatcc ttaccgcgat aggtcagatt cagactgtgg
     3061 ctttcggcgg tatcgaccac gcgaatagtc acttccgatt cttcctgaaa ctgcgggatc
     3121 accgcattca gccatgtttg aaactggctc tcttccggta acccggaatt atcttcacat
     3181 gccagttgta aatcgaggat cacctgactc atttttgttc ctgttcttcg cgcttgcgtt
     3241 ctgccgccag cgccgctttt cgtttttgtt cggcttcttc ccaggcttca taggcgttaa
     3301 cgatacgcgc caccacaggg tgacgaacca cgtcttcgct gtggaagaag ttaaagctga
     3361 tctcttcgac atcggccagt acttcgatgg cgtgacgtaa gcctgattta gtattacgcg
     3421 gcaggtcgat ctgtgtgacg tcgccggtga taaccgcttt tgagttaaaa ccgatacggg
     3481 tcaggaacat cttcatctgt tcgatggtgg tgttctggct ctcatcgaga atgataaacg
     3541 cgtcgttcag cgtacgacca cgcatatagg ccagcggcgc gacttcaata acgttgcgct
     3601 caatcagttt ctcaactttc tcaaagccca gcatttcaaa cagcgcgtcg tacagtgggc
     3661 gcagatacgg gtctactttc tggcttaaat cgccaggcag gaagcccagt ttctcaccgg
     3721 cttcgaccgc cggacgagtc agcagaatac ggcgaatctc ctgacgctcc agggcatcaa
     3781 ctgccgcagc cactgccagg taggttttac ccgtacccgc cgggccaacg ccgaaggtaa
     3841 tgtcatggtc gagaatattg gcgatgtact gcgcctggtt tggcgtgcgc ggcttaatta
     3901 cgccgcgttt ggttttgata ttgaccgctt tgccgtactc cggcacgctc tccgcacttt
     3961 gctccagtac tcgcgcttct ttaatcgtaa ggtggatctg ttccggttcg atatcctgaa
     4021 tctgaccgcg catcggggct gtatcgacat acaggctacg cagaatgtct gccgcagcgg
     4081 tgacgcaaat cggacggccg gtcagtttaa agtggttgtc gcggcgattg atctcgatgc
     4141 cgagacggcg ttcgagctgc ttgatgttgt catcaaacgg gccgcacagg ctcaacagac
     4201 gcgcgttgtc tgctggctcc agggtgattt cgcgagtgtc tatgttcaaa ccgttcctct
     4261 tatctgtatg ccgccggaag ctgaacattc accggcctat aaggaaatta ttcacgccac
     4321 aggaaaaagg cgcaagcatt gcgataaaga tggggataaa gagagaaaaa acaaggccca
     4381 ccggaacggc gggcctgaga attacggctg ataataaccc acgccaaggt cgttttcttt
     4441 gcgggtacgg gcaatcaccg attccggtgt ttctgccacg cgcaggccca tttcatcttc
     4501 agtacgcacc actttaccgc gcagagagtt cgggtagacg tcggtaattt ctacatcgac
     4561 gaatttaccg atcatatccg gtgtgccctc gaagttgacc acgcggttat tttcggtacg
     4621 cccggaaagc tccatgatgc tcttacgcga agtcccttct accagaatac gctgggtggt
     4681 gccgagcatc cggcggctcc atgccatcgc ttgctgattg atacgttctt gcagaatata
     4741 cagacgctgc ttcttctctt cttccggaac atcatcaacc atatcggcgg ctggtgtacc
     4801 cggacgtgca gagaagataa agctgtagct catgtcgaaa ttgacgtcgg caatcagctt
     4861 catcgttttc tcgaagtctt cggtggtttc gccagggaag ccaacgatga aatcagaact
     4921 gatctgaata tctggacgcg ccgcacgcag tttacggatg atcgctttgt actccagtgc
     4981 cgtatgagta cggcccatca ggttcagaat gcgatcggaa ccgctctgta ccggcagatg
     5041 caggaagctc accagctccg gcgtgtcgcg atacacttcg atgatatcgt cggtgaattc
     5101 gatcggatgg ctggtggtaa agcgaatacg atcgatcccg tcgatcgcag caaccagacg
     5161 cagcagatcg gcaaacgatc cggtggagcc gtcgtagttt tcaccacgcc aggcgttcac
     5221 gttctgaccg agcaggttga cttcacgcac gccctgagcc gcaagctggg caatctcaaa
     5281 cagaatatcg tcagacggac ggcttacctc ttcaccacgg gtgtaaggca ccacgcagta
     5341 ggtgcaatat ttattgcagc cttccatgat ggagacaaac gcggtcggcc cttcggcgcg
     5401 cggttccggt agacggtcaa acttctcgat ttccgggaag ctgatatcta caaccgggct
     5461 gcggtcgccg cgcacggagt tgatcatctc cggcagacgg tgcagcgttt gcggcccaaa
     5521 aataatatcg acatagtggg cgcgctggcg aatgtgctcg ccttcttgcg atgccacgca
     5581 gccaccgacg ccgataatca ggtctggatt cttctctttt aacagtttcc agcgacccaa
     5641 ctgatggaag actttttcct gagccttctc gcggattgag caggtgttca gcagcagcac
     5701 atccgcttct tccgccacgt cggtcagttg atagccgtgg gtggcatcca gcagatcggc
     5761 catcttcgat gaatcgtact cgttcatctg acagccccag gttttaatat ggagtttttt
     5821 ggtcatcgac ttgctcttgc gaaatagtag ccaggaatgc agggcgcata gtgtaatgct
     5881 ttgctgccgt tgtggccagt atgagcgtta tcagcccttg ggggtaaaaa tcctgtaaac
     5941 ttaaagcata ttgctaacag gatgattgac catgacaaat caaccaacgg aaattgccat
     6001 tgtcggcgga ggaatggtcg gcggcgcgct ggcgctgggg ctggcacagc acggattttc
     6061 ggtaacggtg atcgagcacg cagaacctgc accgtttgtc gctgatagcc aaccggacgt
     6121 gcggatctcg gcgatcagcg ccgcatcggt atcattgctt aaagggttag gggtctggga
     6181 tgcagtacag gctatgcgtt gccatcctta ccgcagactg gaaacgtggg agtgggaaac
     6241 ggcgcatgtg gtgtttgacg ccgctgaact taagctaccg ttgcttggct atatggtgga
     6301 aaacaccgtc ctgcaacagg cgctgtggca ggcgctggaa gcgcatccga aagtaacgtt
     6361 acgtgtgcca ggctcgctga ttgcattaca tcgagataat gatcttcagg agctggaact
     6421 gaagggcggt gaaacgatcc gcgcgaaact ggtgattggt gccgacggcg caaattctca
     6481 ggtgcggcag atggcgggaa ttggcgttca tgcctggcag tatgcgcagt cgtgcatgtt
     6541 gattagcgta cagtgtgaga acgatcccgg cgacagcacc tggcagcaat ttaccccgga
     6601 cggaccgcgt gcgtttctgc cgttgtttga taactgggca tcgctggtgt ggtatgactc
     6661 tccggcgcgt attcgccagt tgcagaatat gaatatggcg caactccagg cggaaatcgc
     6721 gaagcatttc ccgtcgcgtc tgggttacgt tacaccgctt gccgctggtg cgtttccgct
     6781 gacgcgccga catgcgttgc agtatgtgca gccggggctt gcgctggtgg gcgatgccgc
     6841 gcataccatc catccgctgg cggggcaggg ggtgaatctt ggttatcgtg atgtcgatgc
     6901 cctgattgat gttctggtga atgcccgcag ttacggtgaa gcgtgggcca gttatcctgt
     6961 cctcaagcgt taccagatgc ggcgcatggc ggataacttc attatgcaaa gtggtatgga
     7021 tctgttttat gccggattca gtaataatct gccgccgctg cgttttgtgc gtaatctcgg
     7081 gttaatggcg gcagagcgtg ctggcgtgtt gaaacgtcag gcgctgaaat atgcgttagg
     7141 gttgtagcct tacaacattg ccggatgcgt gccaaccgta ggtcggataa gacgcggcag
     7201 cgtcgcatcc gacattgaag gataagacgt gtcaacatcg cattcgacat tgaatgaacg
     7261 cagaaaagca aaaagctcgc cgaagcgagc ttttttaatg tggctggggt acgagg
//
LOCUS       DADLTD010000194         7266 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.196, whole genome shotgun sequence.
ACCESSION   DADLTD010000194 DADLTD010000000
VERSION     DADLTD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.196"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            52..936
                     /locus_tag="JN701_003410"
     CDS             52..936
                     /locus_tag="JN701_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY7365743.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="JN701_003411"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="JN701_003411"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HAY7365744.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /locus_tag="JN701_003412"
     CDS             complement(1931..2677)
                     /locus_tag="JN701_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY7365745.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="JN701_003413"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="JN701_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY7365746.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="JN701_003414"
     CDS             5160..6443
                     /locus_tag="JN701_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY7365747.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..>7266
                     /locus_tag="JN701_003415"
     CDS             6590..>7266
                     /locus_tag="JN701_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7365748.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVV"
BASE COUNT         1922 a         1771 c         1695 g         1878 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgt
//
LOCUS       DADLTD010000195         7214 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLTD010000195 DADLTD010000000
VERSION     DADLTD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.197"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(826..1356)
                     /locus_tag="JN701_003416"
                     /pseudo
     CDS             complement(826..1356)
                     /locus_tag="JN701_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="JN701_003417"
     CDS             complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="JN701_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY7365749.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="JN701_003418"
     CDS             complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="JN701_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY7365750.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="JN701_003419"
     CDS             complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="JN701_003419"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAY7365751.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4355..4768)
                     /gene="hiuH"
                     /locus_tag="JN701_003420"
     CDS             complement(4355..4768)
                     /gene="hiuH"
                     /locus_tag="JN701_003420"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7365752.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4901..5572
                     /gene="hprR"
                     /locus_tag="JN701_003421"
     CDS             4901..5572
                     /gene="hprR"
                     /locus_tag="JN701_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY7365753.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5572..6930
                     /locus_tag="JN701_003422"
     CDS             5572..6930
                     /locus_tag="JN701_003422"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY7365754.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
BASE COUNT         2026 a         1489 c         1631 g         2068 t
ORIGIN      
        1 gctgagaaaa aagcgtgctt tcccaatctg gggcaaatca cgtctgaatg acacctgaaa
       61 acaggtggtg gactcaagaa ggcttggaat attcttcttt aatgttatgt aattcattga
      121 tttttcgtgt acgattttaa gacatttttt ccaagaaaaa tttttaactc tttgattttt
      181 cgactctgta tcatcggtct cgaaaaccgg agtaggggca actctaccgg gggggcaaat
      241 ccccctctct ccgccacttt atcaatgact tatctcccga cttcccgcct tacttttcct
      301 aaacagaaca atcgtagaat attcttgaag ggttagatcg tcactgtttt ctgttcgata
      361 ctgtgacatt cagcacttga ttcgctatgg atctgacagg aagctttcga gcgaaaatgt
      421 gcagttattc agtcgttttc ttatcgctca ccattattct tttagacatt catcctatga
      481 aactgccgca aagttggtgg tgggaactga agttgcgtag agaaggggtc aataattgag
      541 tcaaaaaagg aacgcggccg tcagattaaa gccgcgggac aaagtccata atttccaaga
      601 gttgttgaat ttcccagctt ttttgaaagc ctttgtatca tactaacaag taaaaaaggt
      661 ttaaatacca aaattaacct gtatttttga taagtcttac cattgtacga tttacacagt
      721 ttctgctcct ccttgttgat agtggaaagg gtgcattgtg cacttgcgta aacatttatg
      781 acgcgttgat gtccatggtt cacatttcct tgtcagggga tcgacttatt tgcggcgagg
      841 catcattcgt aacagagtat tatctttctg aatgtagtga tgaaagagtg ctgcgccagc
      901 gtgagctgcg attaaaaagt aacctatatt tgcccaggtt tcgtgaatat tttatatcag
      961 tgctttaatt tcgctgtttg gggtaacaaa gggagacaca ttgaaaccaa ggaaactcca
     1021 cgattttcca ctgtaagcca tcaaagcaat ccccagcaga ggaagtgcaa ggaaggttat
     1081 ataaagcatg atgtgcatta gcgaagccgc tttcatttgc caggcaggtg gcggtggcac
     1141 aatagaagga tcactataac ggtgttttat aatcaggcgt gaaaacatta acacccaaac
     1201 gaatataccc gcattgtaat gtgtttctcg catcagcaga taagtactac tacctttagg
     1261 aaaccagcca cggagttcca tcgcggcata ggttattgca acgaaaagca gcgttatcca
     1321 atgtaaataa atttgagttt ttgagaatcg gttcattttt gtagctcctg taatttttac
     1381 agtcaagcta tcccacgaac cttaaaaatt ccttatacag atgtggtttt aagaatttgt
     1441 tatttaaaaa attgatagtg cacattgtaa ccagccaata taagcaatgg cgtgagcaca
     1501 ggtatctttc tctgttggcc gtattgtgta tggaatccgt tattggttaa ctcaatgcgg
     1561 gttagcttat taatatacag acacgtcata gtgatgagcg tgtctgtagc tagcttgcgt
     1621 tcagtggcgt ttgtggatgg accagtggca gttatgatga cttactaaag cggcatcgag
     1681 gcgttatcat gagaatactc aatgagacat catttcctcg accacttctt cgctactcaa
     1741 ctgatatgga taatacgttg gccagttttc catttcattt aataaagatt gctgtgaatc
     1801 attacccata aaaatgtgaa aatgagatga ttttcgtggt gcaattatat gatcgctaaa
     1861 ttgtatatat ttaggggctt tgctttcagg atctttacat tcgaataagt aacgaacgcc
     1921 tttcttgcct gatttatagg tgagtatttt gtatccatcg taatcatatt tacaggatgt
     1981 tgtttcatta tttctatgga attcaacaat gccgtcctca atgccaatca tctcgatatc
     2041 tgttgcataa cctttgtgat aataatcttt aatttcagca aatgttttag ttttatctgc
     2101 atccgctttc ttctgaaaga cggggtcaag tttgccactt tgcagtaaag gataaacgga
     2161 ttgccagacc ccatcccagt cactgagcgt tcggttttgt acattggcat catcaaaaac
     2221 accattagca gctttttgtt cgacctctgt taagggtttg ccgtgtgagt gatgaccatg
     2281 cgaaaaggca ggagcgctaa caataaagac acctaacgca acagccagtt tgtgaagacg
     2341 aatcgccaaa acagtttcct ccagagtcat tgtatatgat gtgtaatgtt atattgtaac
     2401 atatagcaaa atgcaacaac tcttaggaga tgagagcata gttttctcag gtgtacttat
     2461 ctatcttatt gaaataaaat gaattatatt gataatttga ggttgatgac aaacgaaaca
     2521 taatctgatg tgatctgcgt aggccggata gggcgctcgc gccgcatccg gcagggataa
     2581 cgcgtacttt gtcggcaata tgaagttata cagataattt gttgtgaacc tgcttgcgta
     2641 accggttaaa aagggaacgc aacttcttat accgtaaggc taaaagcagt acagccagcc
     2701 cagcgtagat gagcggctgc ggtgaaataa tcttcactga ccacagataa tgtattgggg
     2761 ccaggatcgc gacaagatag acgaaattat gcaactgttg ccagtgcttg ccgagttttc
     2821 gctgcatcgc ctgggttgaa gtaaacgcta aagcaagcaa aataacccag ctgataattc
     2881 ctaacgttaa ataaggccgg gtaattaact ccttgcctaa taacgccaga ttattcacgc
     2941 ccagctccag caatgcgtaa ctggttaaat gcagtgtcgc ccaggcaaag caccataagc
     3001 ctaacaggcg gcgagtgcgt atcaataacg gctgttttgc gtagcgtgcc agaggtgtga
     3061 ttaacaaggt cgccagcaaa aatttcagtg cagtgcgacc ggtaaaatgc tgaatatctt
     3121 tcaccggatc ggcacccagt ccaccgtgat tgatcgccca gaccagccag agaaacggca
     3181 acaatccggc aagatgcagg ctaactttca gccatgtcac ctgttttgct gtcagacgca
     3241 ttcagaaatt ctcccgcaaa tccaggccac gatacagcga tgccacctgg tcggcgtaac
     3301 cattaaacag tagcgttggc tggcgctgaa catcgaggat gccgcctgaa ccaataaatc
     3361 gttcggtagc ctgtgaccag cgcgggtgat caacatgcgg attaacgttg gcgtaaaaac
     3421 cgtattcgtc aggcgctgcc agattccagg tggttggcgg acgctcgcgg gtcagcttaa
     3481 tactgactat cgatttaatc cctttaaagc catatttcca cggcacaatc agtcgtaccg
     3541 gcgcgccatt ttgtggcggt aacgccttgc cataaacacc cacggtcatc agtgtgagcg
     3601 gatgcattgc ttcgtcgaga cgcaatcctt cgacataagg atatttcagc ccgccgccga
     3661 taaagcggtc ctgctggcct ggcatttgtt ccggtgcata aattgtttcg aaagcgacat
     3721 acttcgcatt gctggtgggt tcggcaagcg ccagcaattt gtgcagcgga aaaccaatcc
     3781 acggcaccac catcgaccat gcctccacgc agcgcatacg ataaatacgc tcttccagcg
     3841 ggaagcgacg ggttaaatca tcgtgatcga gggtcaatgg ttttgccact tcgccgctga
     3901 ttttcagtgt ccatggatcg gttttcaggc taccagcatt agcggcggga tcggctttat
     3961 ccagcccgaa ttcatagaag ttgttataac cggagacttt atctactggc gtcagtggca
     4021 ggttattttg ccaggcggca ggcttgctga actccagcgg ttttccggcg ggggcgggcg
     4081 ggcgatcgtt ccctttaaac cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa
     4141 gtgcagctgc actgatgccc agtgctttta acacctgccg acgcttcata aagaataccg
     4201 actcggccgt aacatctgat tcttttaaaa attgattctt tttcataagc catcacactt
     4261 ttttgtcgtt agccagcaag agccgtttac tgttaatctg tcagggagtg tcctccctaa
     4321 cagtagcaga gatctgcttt tacatattta tgcattaact gccacgatag gttgaatacc
     4381 catattggct aagtaataaa ggcacatgat aatgctcgtt cactttatta atatgaaatt
     4441 caacggggat ctcagggaag aaactttcaa gattttgttt cttgaaatag tccccggttt
     4501 taaatacgac acggtaatcg cccgtagttg cagtttgctc gggccacagt gctttaattc
     4561 gtccatcctt atctgttttg gcggtattaa gttgtaacca gccgttatcc gccttctttt
     4621 caagagtgac tgtcacgtca gcagcgggtt ttccggtttg ctggttcaaa atgtgcacgc
     4681 taagaatgtt ttgttgtgcg gcataaacca aagaaggtaa tgaaaatgct gccgttgcta
     4741 ccgagagtac taaataacgc tttaacatgt ttatatcctt gtcatgtgaa tgagttttga
     4801 ggatagcgaa aaagcggcca gggtaacctt acagcagcat tacaattttg taatgaacca
     4861 ggctgtttct ataacatatg atttatggca tattattttc atgaagattt tacttattga
     4921 agataatcaa aggacccagg agtgggtaac gcaggggctt tccgaagcgg gttatgtcat
     4981 tgatgccgtt tctgatggca gagatgggct ttatcttgcg ctgaaggatg attatgcatt
     5041 gataattctg gatattatgc ttccgggtat ggatggctgg cagatcttac aaacgttaag
     5101 aacagcaaag caaacccctg ttatttgcct tactgcaagg gattctgtcg atgacagagt
     5161 cagagggctg gacagtggag caaatgatta tctggtaaaa cctttttcat tttctgagtt
     5221 gctggcaagg gttcgggcac aattaaggca acatcacgct ttgaattcaa cattagaaat
     5281 cagtggctta agaatggact ctgtaagtca aagtgtgagc agggacaata tcagtattac
     5341 actgacgcgc aaggagtttc agttactttg gctactggcc tccagagctg gcgaaattat
     5401 acccagaacg gttattgcga gtgaaatttg gggaatcaac tttgatagtg ataccaatac
     5461 ggtggatgtc gccattcgca ggctccgcgc aaaagttgat gatccttttc ctgaaaagct
     5521 aatcgccaca atccggggga tgggctattc attcgtagcg gtaaaaaaat aatgaaaaga
     5581 ttgtctataa ccgtccgttt aaccttgctt tttatattgc tgctgtctgt tgctggcgct
     5641 ggaattgtct ggactctcta taatggcctg gcaagtgagt tgaaatggcg cgatgataca
     5701 acactcatta accggacagc acagatcaag cagttgttaa ttgatggggt aaatccagat
     5761 acgttacctg tatactttaa ccggatgatg gatgttagtc aggatatctt gatcattcat
     5821 ggtgatggca tcaataaaat tgttaaccgg acaaatgtca gtgatgacat gttaaataac
     5881 atacctgcta gtgagacaat cagcgcagct ggcatttaca gaagcattat taatgatact
     5941 gagattgatg ctttacgaat taatattgat gaagtttcgc catcattaac ggttactgtg
     6001 gctaaattgg cttcagctag acataacatg cttgaacagt ataaaattaa tagcattata
     6061 atttgcattg tcgccattgt actttgctca gtattaagtc cgctgttaat cagaacggga
     6121 ttacgagaga tcaaaaagtt gagtggtgta acggaagcgc tgaattataa cgatagccga
     6181 gagcctgttg aggttagcgc attaccgaga gaactaaaac ctcttgggca ggcgttgaat
     6241 aaaatgcatc aagccttagt caaagatttt gaacgcctaa gtcaatttgc tgacgatctc
     6301 gctcatgaac ttagaacgcc cattaatgca ttactgggtc agaatcaggt tacgctcagt
     6361 caaaccagaa gtatcgctga atatcaaaaa acaattgccg gtaacattga agagttggaa
     6421 aatatttcgc ggttaacaga gaacatactg tttcttgccc gggcagataa aaacaatgtt
     6481 ttggtgaaac tggactcgct ttctctcaat aaggaagtcg aaaatttgtt ggattatctt
     6541 gaatatcttt cagacgagaa agagatttgc tttaaggtcg agtgcaatca gcaaatcttt
     6601 gcggataaaa ttttactgca acgaatgtta tcgaatctta ttgttaatgc cattagatat
     6661 tcaccagaaa aatcgcgtat tcatataacc agttttcttg ataccaacgg ctatcttaat
     6721 attgatatcg ccagtcttgg aacgaaaata catgagcctg aaaaactctt ccgtagattt
     6781 tggcggggag ataattcgcg tcattccgta ggtcagggac ttggtctttc tttagtcaaa
     6841 gcgattgccg aattgcatgg gggaagtgct tcatatcact atctcaataa gcataatgtg
     6901 ttccgaatta cgttaccgca aagaaattaa catagggctt cagtacgcca taaattaacc
     6961 gtgaaaaaac gcagccacgt cgtgagtact aacgcggctg cgattaatta tgcgcttaca
     7021 ttcaaacgta acagggatta acccgcgtaa gctgccagca tttcctgcgc cgccagttta
     7081 cccaacgcat tcgctgcgaa aggactgtcg ccggtgagaa gtttacggtc cttatgtact
     7141 cgcccggtga tgtcgtcatt aatgatgttc atgcccattt tcttcagctc ttcgccgaag
     7201 taccaggtga gatg
//
LOCUS       DADLTD010000196         7202 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.198, whole genome shotgun sequence.
ACCESSION   DADLTD010000196 DADLTD010000000
VERSION     DADLTD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.198"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(235..789)
                     /locus_tag="JN701_003423"
                     /pseudo
     CDS             complement(235..789)
                     /locus_tag="JN701_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            932..1054
                     /locus_tag="JN701_003424"
                     /pseudo
     CDS             932..1054
                     /locus_tag="JN701_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1316..1882)
                     /locus_tag="JN701_003425"
     CDS             complement(1316..1882)
                     /locus_tag="JN701_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365755.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2164..2478)
                     /locus_tag="JN701_003426"
     CDS             complement(2164..2478)
                     /locus_tag="JN701_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365756.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2556..2666)
                     /locus_tag="JN701_003427"
                     /pseudo
     CDS             complement(2556..2666)
                     /locus_tag="JN701_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="JN701_003428"
     CDS             complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="JN701_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY7365757.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4039..4176)
                     /locus_tag="JN701_003429"
                     /pseudo
     CDS             complement(4039..4176)
                     /locus_tag="JN701_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4258..4556)
                     /locus_tag="JN701_003430"
                     /pseudo
     CDS             complement(4258..4556)
                     /locus_tag="JN701_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5145..5304)
                     /locus_tag="JN701_003431"
                     /pseudo
     CDS             complement(5145..5304)
                     /locus_tag="JN701_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5308..6462)
                     /locus_tag="JN701_003432"
                     /pseudo
     CDS             complement(5308..6462)
                     /locus_tag="JN701_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6482..6829)
                     /gene="tnpB"
                     /locus_tag="JN701_003433"
     CDS             complement(6482..6829)
                     /gene="tnpB"
                     /locus_tag="JN701_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7365758.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6829..>7202)
                     /locus_tag="JN701_003434"
     CDS             complement(6829..>7202)
                     /locus_tag="JN701_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7365759.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         1859 a         1677 c         1555 g         2111 t
ORIGIN      
        1 aaaccttctc atgtttagag ttttttatta tgtttatcat aatgatatga cgatctgagt
       61 ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac agtgcctgtg gataactttg
      121 cgtaaatccg gaggacagat cactctgaag caagacaaca gcatctgaaa acaatatgct
      181 cacgtttcaa ccacagggcg taagtgatcc tacccacgta atatggacac aggcctaagc
      241 gaggttctgg ttttcaaatt gttccggact gaggccgcca caccaactgt gccgtcgcca
      301 gcgattgtaa tcacattcga tataattaaa cactgttgcc cgcattattt cccggctgat
      361 aaagtgttct ccgtggagac attccacttt cagcgaatga aagaagcttt ccacgcaggc
      421 attatcgtag cagcagccct ttgcgctcat actgccacgc agattatgcc acttcagcag
      481 cgcctgataa tctgctgaac agtactgact tcctcggtcc gagtgaacga taacgttccg
      541 gggtctctta cgccgccaca gcgccatctg cagggcatcg caggccagtt gcgccgtcag
      601 gcgtggtgac atggaccagc caataacggc acgtgaccac aggtcaatga ccacagccag
      661 atacagccag ccttcatctg tacgtaagta cgtgatgtct cccgcccact tctggttcgg
      721 accactggcg taaaaatcct gctccagcag attttctgac acaggcaggc catgtgcgcg
      781 gtaactgacc gtattgctgg gtaagatcaa tttgctcact ggtatttact acgtgcatcg
      841 aatgagtgat caagagccgt atgacgcgag agtgtcacgt acggttccgt gagcagccgg
      901 agagtgaaac tctctccggc tgactcgact cgcaggttcc gacagcggtg acgaacatgc
      961 agcggtgttg ttctcgctga tcggcacatg ccgtctgaac aatgtggagc cagaaaaatg
     1021 gctgcgttac gttattgaac atatccagga ctgaccggca aaccgggtac gcgatctgtt
     1081 gccttggaaa gttgatctga cttctcagta aatatcaata tgattctgac gagccgctta
     1141 cctttgagca gagaaagggg agagggatcc cacatccatg agctaggaaa atacaaaaac
     1201 tggctataca acaattcaat atattaggac ttacttgcga ggagaaatat agcaccgctc
     1261 aggcaattcc tgggtattta cttttcttag tttctttact cgggatgttc tgtcatcaga
     1321 aaggcgattc taaatttgta atataatcac tcatattccc acgtaatttc tttagatcaa
     1381 tatattgcac atttttctca aaaaactgat cggaaataag atttgcaaca ttgcttgttg
     1441 tatcattttt aacgcttcta ttcatccctt cacaaagttc gtccattttc tttttgaaat
     1501 aactgcttcc tgatacaaga tgatttatgg aactttgtgc agcattgatg tctagagaaa
     1561 tgtgataatc tttctcaacc tgtttaaata tctgtttacg gagattatcg ccgatttttc
     1621 ggctgtgttc tgcacaaaac ttatcaatac attggtttac cacccgggat acgtagactt
     1681 ttttttcatc atttttccaa tctgtaatat tttcaccgat ttgttctgaa acagagctca
     1741 aaatactagt atttattttg gttgatgata tttttactat ccttttcccc attgtacagc
     1801 ggataatctc atcaaccttt cctgaaaaat atcttttgtg agataaactg attccaaaat
     1861 tattaaaaga tgttccaagc attataatca ctttgtcatg taaaatagta acattgtcat
     1921 tattttagct gattcaaaca attctgtaaa tgctttgagt tctttactcc cttggctcaa
     1981 tgatggaccg tctggaatcc cagagactaa actctctgag aaagagagta tgaaaaaact
     2041 tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat aaccggcatc attcataatg
     2101 atgccctgtt agttgtagtt atgaagattt acgtcattaa aattgctgtt cgtagtgtca
     2161 gtcctatggt ctggcatcag ctgaggatcg tagccgatac atcactggct tcacgacatt
     2221 cgtgttgagg ctattcatga aaactccacg ctgaaaacgc ctttttgtat aaacggtcac
     2281 ggtatgcccg gagccactgt tgctgatgaa ttcgataaaa ccttggcgtt tcttgaagct
     2341 atcgttaatg cggataatga aacaacgatt ggtgagattc gctcttttgc cgacaccctg
     2401 ggcgctgtcc ggttcaatcg caacaaaatc aaccggcagc tgagcaaacc caaccttgcc
     2461 tctctggcgc tggagcatga agtgatttag ctgggacgta gccactgaca gcttctataa
     2521 aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg gttttcaaat
     2581 tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta atcgcacttg
     2641 atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg gctttatgca
     2701 ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc ctgtatttcc
     2761 cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta catttattgt
     2821 gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg ttctatgggt
     2881 agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag atcacacaat
     2941 tcggggagag ctccttctaa gtgcaattct ttgcagctat ttagcacact taaaacaact
     3001 cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta agtctgtctg
     3061 ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc atcatagcct
     3121 cgatattccc tttccctgcg gccagaaata atcctggtaa cccctcatta tctcttacat
     3181 ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa tctccataag
     3241 cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat tccccagcat
     3301 tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct gccaacagcg
     3361 attcaccgta tttacgcgct gcatcagcat ttccatgcca aatcaatgaa aacaaattat
     3421 ggcggttata ctccttatta ttctttgtcg gaatggatat gctctgtatt ttggccaatt
     3481 ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg gcgtgggata
     3541 tagcggtatt tgtcacgtta atgatattac gtttcattcc tttattttca ttagcagtat
     3601 tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca tagttattta
     3661 ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat aacctatttt tgtaatgtaa
     3721 tagatattag tctattgcta atctaaaaaa accattgcca cattgaatgg ctatattcac
     3781 caataaattc tttcgatttc gtatattgac atatcgtaga tatatcttat ccctaagatt
     3841 caataatcga gtccgtttac cattctgtgc aagcgccttc actcagaagt gaccgtcaag
     3901 gcgatcacca aacttgataa taaagcagct cattgccata cgccagtcct taaggggcgt
     3961 tgtccatttc tgtgatgctg cctagattta actgcctgct ccggcttatc tacatctaaa
     4021 tatgtgagcc taataccgct acactttggc cagactatgt ttttctccgg gaagtgggct
     4081 aaccgtctcc ataaagtgac gaactttttt caacagtggc catattgaac ggcactgatg
     4141 attacgggtt attgtctcat gaagagccgg ccacatcact gacagcagtg acttcaggcc
     4201 ttcagcacca gacagcctga accagttaat ccatcgcccg acagatttag taatctaggg
     4261 ctggtttttc ttcagctgtt gcggttcgaa agtgccatta cgatcacgca gagtacggat
     4321 ttccagaggt tcatctccag tagtgataat cttcgttaag caaccattgc gggaattgct
     4381 gccagatcta agctgatttt tctcgcaccc aagatgatga gtcatattag cattgagtac
     4441 ggtttcattt tcagcatcca atcaaactga ctgagatctt caggattttt gagacttttg
     4501 gctagttcat tagctaaagc ccgcaactta ttagatgtca actgtttttc attcataaat
     4561 tcaccttcat ttgatatcaa attaaatagt tcaacatcag agaattatac aaacttatgt
     4621 acaggctcct atgatcttcg aatcctgaag attatagtga aaataagttt tcacgttgaa
     4681 atcccacata gttagtatat gataaataat catggatgac ctgattaacc tcttggggta
     4741 gtgcttttat agaagaataa atattctact aatttaatac atcacctaaa atagtattgt
     4801 tttttatggt tggttgaagg tcgtggtgat gatgttagtc cttttgagaa tattccagtg
     4861 ccagatctta cttataataa aggggcttgg cagtttggat aaactattgt tcttattcca
     4921 ctaatacaaa ctttttgtaa agtatattaa atatacttca tttcatactg aaatccccgg
     4981 aagtattcaa tcaaacaatt acaccataaa aggaagaggg ggccctaatt aagttgtcaa
     5041 gcatgtttat gatacctgca gggtataaat tttctggctg caagcagaca catataaaac
     5101 gtctttattg atcctaccca cataatatgg acacagtcgt aagtgaggct ctggttttca
     5161 aattgttccg gactgagacc accacaccaa cggtgccgtc gccagcgatt gtaatcgcac
     5221 tcgatataac taaacactgt tgcccgcact atttcccgat ggataatagt gttctccgtg
     5281 gagacattcc attttcagcg aatgaatttt catctgttgc tgtatccagt catacagtga
     5341 ctgcatcagt ggcgcggctc tggcttttct tgccgccaga cgctgttctg ctgaacatcc
     5401 ccggacttct gcctcgatgg catacagttc accgatacgc agcagggctt ccgttattct
     5461 gccggattcg tacaacgccc ggtaaccacc gtaagcatcg gcctgaagca ctccgctgta
     5521 accggccaga tgattctgtg gatggatacc tttccggtcc ggtgagtacg cgaaccagac
     5581 cgccggaggc atttccgaac cggcgttacg gtcatcacgg acatagaccc acagccgggc
     5641 agttcttgtt ttaccactgc ccggctcctg gaccggaacg gggatatcat ccgcatgaac
     5701 tttaccgggc atcagcacat actggcgcag gacgtcatac agcggctcca gcagttcagc
     5761 aacggcaccg gtccagcgac ccagtgtggc acggctcagc tccacgccct gacgacgtta
     5821 tatttctgac tggcggtata acggcagata gcctgcatat tttccagcga caacatgggc
     5881 cagaagcccc gctccggcat aactgcgtgc aatgggtttt gaaggcaccg gtgcctgcac
     5941 gatatggtca caccggcaac aggccagttt cggacgttgt gtttcgataa ccttaaaggc
     6001 gctgctgata agctccagct gctctgacac atcacatccc agagaactga gttcgccacc
     6061 acaggccggg cagcattcct cttccggacg gatgacccgg gtttcacggg gaagtgaggc
     6121 aggtaacggt ttacgggctg aagactggcg caggggagat ggcagtaccg ggtcatattg
     6181 ctcacccagc gtttccgcca tttcttcctg aagtgcgctg attcgctcct gtgcttccag
     6241 tatctgccgt tcggtttttg cgcgaagttt ttctgagctt taaccgaact gcatgcgctg
     6301 cagtttcgca accagcgcct tcagccggtt gatttcggaa gcataagccg ccacccgctg
     6361 tgagagcagg cggttgtatt tcgccatctg gcggatggtg tcctgttgcg tctgcaacag
     6421 tgcccgcagg cgggcattct catgagcaag tgaggtgtcc atatcctcac tttacaacgg
     6481 gttatatgcg gatgccagcg cgttccgttc gtttcgggtg tttccagtcg atgccctcaa
     6541 gaagcatgga taactgggcc ggagtaaggt gcaccttgcc gtcacgggtg accggccaga
     6601 cgaagcggcc ccgctccagg cgtctggtaa agaggcacag tccgtcactg tcagcccaca
     6661 acacttttat ctggtcaccc cggcgtccgc ggaagatgaa cagatgcccg gagaacgggt
     6721 catccttcag gacgttctga acttttgagg ccaggccgtt aaagccattt cgcatatcgg
     6781 tgatacctgc aaccagccag atacgcgaac ctgcagggaa agatatcatc agtggctgct
     6841 cccttttatt tcgcggataa gtatctgtaa taacgccggc gtcagtttac ctttaagcct
     6901 gagagttccg gccggcagaa ccagctcaca gcacagactg tcggacggtg tatttatctg
     6961 ctctggttcc tgtgccggag ccgggatttt attatccggc tccggcgtta acgtcacggg
     7021 aagcagtgca ggcatatttt ttccggaagg cagcaggcca cctttccggt attgatggcg
     7081 ccagttgaag agcaggttat cgttgattcc gttttcacgg gcgatctgcg ccacacaggc
     7141 tccgggctgc agtgactgct ccactaaggc gattttaaat tcataaggaa aattaggccg
     7201 cc
//
LOCUS       DADLTD010000197         7120 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLTD010000197 DADLTD010000000
VERSION     DADLTD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.199"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..648
                     /locus_tag="JN701_003435"
     CDS             <1..648
                     /locus_tag="JN701_003435"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY7365760.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            739..1986
                     /gene="tnaB"
                     /locus_tag="JN701_003436"
     CDS             739..1986
                     /gene="tnaB"
                     /locus_tag="JN701_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY7365761.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2118..3293
                     /gene="mdtL"
                     /locus_tag="JN701_003437"
     CDS             2118..3293
                     /gene="mdtL"
                     /locus_tag="JN701_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY7365762.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3268..4227
                     /gene="yidZ"
                     /locus_tag="JN701_003438"
     CDS             3268..4227
                     /gene="yidZ"
                     /locus_tag="JN701_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY7365763.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4384..5133
                     /locus_tag="JN701_003439"
     CDS             4384..5133
                     /locus_tag="JN701_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365764.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5155..5721
                     /gene="yieF"
                     /locus_tag="JN701_003440"
     CDS             5155..5721
                     /gene="yieF"
                     /locus_tag="JN701_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY7365765.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5775..7112)
                     /locus_tag="JN701_003441"
     CDS             complement(5775..7112)
                     /locus_tag="JN701_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7365766.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     IMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1693 a         1822 c         1802 g         1803 t
ORIGIN      
        1 acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat ggtgccgatg
       61 ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg tgtacaccga gtgcagaacc
      121 ctttgcgtgg tgcaggaagg cttcccgaca tatggcggcc tggaaggcgg cgcgatggag
      181 cgtctggcgg taggtctgta tgacggcatg aatctcgact ggctggctta tcgtatcgcg
      241 caggtacagt atctggtcga tggtctggaa gagattggcg ttgtctgcca gcaggcgggc
      301 ggtcacgcgg cattcgttga tgccggtaaa ctgctgccga atatcccggc agaccagttc
      361 ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt
      421 ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg cccggctgaa
      481 ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga cttcattatt
      541 gaagccttta aacatgtgaa agagaacgcg gcgaatatta aaggattaac ctttacctac
      601 gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt aatactacag
      661 agtggctata aggatgttag ccactctctt accctacatc ctcaataaca aaaatagcct
      721 tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc attttggggt
      781 gttatggtta tagcaggtac agtaattggt ggaggtatgt ttgctttacc tgttgatctt
      841 gccggtgcct ggtttttctg gggtgccttt atccttatca ttgcctggtt ttcaatgctt
      901 cattccgggt tattgttatt agaagcaaat ttaaattatc ctgtcggctc cagttttaac
      961 accatcacca aagatttaat cggtaacacc tggaacatta tcagcggtat taccgttgcc
     1021 ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
     1081 acgatatcaa tgaatttggg ctatcacgct aatccacgta ttgtcgggat ctgcacagcc
     1141 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgcat tacctcattg
     1201 ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt cttccaggtc
     1261 gattactcca tcctgcgcga tgccaccagc accactgcgg gaacgtctta cttcccgtat
     1321 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
     1381 ctgattattt gctatggaaa acgcaaagat aagttaatca aaagcgtggt atttggttcg
     1441 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tattccgcgc
     1501 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
     1561 ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc taacttagct
     1621 gttgccagtt cgttctttgg tgtcacgctg gggttgttcg attatctggc ggacctgttt
     1681 aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
     1741 gcgttgttgt atctgatctt cccgaacggc tttatttacg ggatcggcgg tgccgggctg
     1801 tgcgccacca tctgggcggt cattattccc gcagtgcttg caatcaaagc tcgcaagaag
     1861 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
     1921 tttggtataa ccgtgatttt gtgctggttc ggcaacgtct ttaacgtgtt acctaaattt
     1981 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
     2041 tgtccaaatg gcaactcgcc tgatcctcct tcaccacgta tgctttgcgt caccttacta
     2101 tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg gttttacttt
     2161 atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
     2221 ccagcgaagc gcagttgcat attgcgttct ccgtatatct ggcggggatg gcagctgcga
     2281 tgttatttgc cggtaaagtg gccgatcgtt cagggagaaa gccggtcgcc atacccggcg
     2341 cggcgctatt tattattgcc tcggtgttct gttcactggc tgaaaccagc acgttatttc
     2401 ttgcaggccg atttctacag gggttgggcg caggctgttg ttacgtagtg gcgttcgcca
     2461 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcatta ctcaacggta
     2521 ttacctgcat cattccggtg ttagcgccag tgctcggaca tctgattatg cttaaattcc
     2581 cgtggcagag tctgttctgg gcgatggcaa tgatgggcat cgcggtactg atgttgtctt
     2641 tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa
     2701 atagcgagtc gctgcttaat cggtttttcc tcagccgtgt tgttatcacc accctcagcg
     2761 tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa atcatgggtt
     2821 ttgagcgcgg agaatacgcc accattatgg cgttgaccgc tggcgtcagc atgaccgttt
     2881 cattctccac gccatttgcg ctgggaattt ttaagccacg tacgttgatg atcacctcgc
     2941 aggtgttatt ccttgcagcg gggatcaccc ttgccgtttc accttcccat gcggtttctc
     3001 tgtttggtat cacactgatt tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc
     3061 aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
     3121 aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc ggcgcatgga
     3181 atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg attatgttcg
     3241 tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
     3301 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
     3361 atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
     3421 gacccgctct ttgtgaactc accgctgggg ctgtcgccca caccgctaat ggtcagcatg
     3481 gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa accgcaccac
     3541 cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat gatgatcatg
     3601 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaactacgt
     3661 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
     3721 ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt agccattgat
     3781 tatgaagtgc tgtttagtga tgtaccctgc gtctggttac gcaaagatca tccggcactg
     3841 catgaaacgt ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
     3901 cagagcgaca cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt
     3961 gctatgagcc tgccggaatt tgagcagtca ctgtttatgg cagcgcaacc cgacaatctg
     4021 ctactggcga ccgcgccgcg ctactgtcag tactacaatc aactccatca actgccgttg
     4081 gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt tccttttacc
     4141 ctgctgtggc ataaacggaa cagtcataat ccgaagatcg tctggttacg ggaaaccatt
     4201 aaaaaccttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc aatttcacaa
     4261 taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg
     4321 tccatcattc cagtaaatga ttactcttat attcataatg gaccattaag catggagcga
     4381 aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt
     4441 ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt
     4501 ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg
     4561 ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg
     4621 ttttctattt cctatgccgg gaatatggtt ggcgtggcgt taacaaccga aggtgaatgt
     4681 ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat
     4741 aaccacacct tttccagcaa tgaatcgcta tggatcagta aacaaaacga tcctaacgaa
     4801 gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg tgatgttttg
     4861 aatgacgatc cgcgcgatct gcagctgctg cccattgccg gacgcctgaa atgtgctcat
     4921 gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc tggtgtggtc cgtggcggtc
     4981 acgcccgcga ttgaaaagct cagtgtctgg gagttagatg gcaaacacag ctggaaaagc
     5041 ctgccggata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg ttttgcccag
     5101 ctctctaccg tgaagtcttt ttcgccaaat tgatagacaa ccacaggagt cattatgtct
     5161 gaaaaattgc aggtagttac gttactgggg agcctgcgca aaggctcatt taatggcatg
     5221 gttgcacgta ccctgccgaa aattgctccg gcgagcatgg aagtcaatgc gttaccttcc
     5281 attgccgaca ttcccctgta tgacgctgac gtacagcagg aagaaggttt tccagcaacg
     5341 gttgaagctc tggcggaaca gatccgtcag gctgacggtg tggtgatcgt cacgccggaa
     5401 tataactact cggtaccggg tgggctgaaa aatgccatcg actggctttc ccgcctgccg
     5461 gatcaaccgc tggccggtaa accggtgttg attcagacca gctcaatggg cgtgattggc
     5521 ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc aatggtgatg
     5581 aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatcggca aaccggagaa
     5641 gtgattgatc agggtacgct ggaccacctg accgggcaat tgaccgcatt tggtgagttt
     5701 attcagcgag ttaagatcta aataaaaaac ccgccagtaa tcatgcatgg cgggttttta
     5761 atgcgctatc gattttaatg agcgtcgata aatacaatct tcaggataaa cagcagcgca
     5821 acgatgatta cgcacgggct aaggtcacgc agacgcccgg taccaatctt catcacgcag
     5881 taggagataa agcccagcgc aataccttcg gtaatcgaaa agctgaacgg cattatcacg
     5941 gcggtaataa acgccggaac agattcagta agatcctgcc agttcacgcg agcaagactt
     6001 gaggtcatca acacgccaac gtagatcaac gcgccagctg cagcgtagcc tggcaccatt
     6061 cccgccagcg gcgacagaaa gataaccagc aggaacagca gaccaacaac cactgccgtc
     6121 agaccggtac gaccgccaac cgatacgccg gaagaggact caatataagc cgtaacggaa
     6181 gaagtaccga taaacgaacc ggtcacggaa gagatactgt cgacatacag cgcctgcttc
     6241 atgcgcggga atttcccctt ctcatccgcc agacctgctt tatcggtcac gccaatcagc
     6301 gtaccggagg agtcaaacaa gttgaccaac atgaaagaga aaatcacccc tgccagcccg
     6361 aggttaaacg acccggctaa atctacatga cccacaactg tcattacgct cggcggcgca
     6421 gaaacgatgc cattgtagtg cacatcaccc agcatccagc ccagcagcgt cgtcaccacg
     6481 atagaaacca gcaccgctgc gtgaatgttg cgcgaggcca gaatagcaat gatgaagaag
     6541 ccgaggatac ccagaagtac gctgtgagaa gtcagattac cgatgctcac cagcgtttcc
     6601 gagttagcga caatcacacc tgcgtttttc agccccatca tgccgataaa caggccgata
     6661 ccgctggtaa tacccacacg cagacttacc ggaatgttag caatcatcca gtaacgaacg
     6721 cggaaaatgg tcaaaagaag cagtccgaca gcgccccaga agattgcgcc cataccgatc
     6781 tgccacggca gccccattgc ctggacaacc acaaaagcga aaaaggcgtt aagccccatc
     6841 gcaggagcca gcgccaccgg aaggtttgca aacaatccca tcataatgct accgaaagca
     6901 gcaatcagac aggttgttac gaagacggca ctggtgtcca tgccagcaac gcccagaatt
     6961 tgtgggttaa caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc ggcgatcact
     7021 tcggtccgtg ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag catcccctgg
     7081 ccagaagcct gggttgtgtg ttgttgactc attatctatt
//
LOCUS       DADLTD010000198         7057 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLTD010000198 DADLTD010000000
VERSION     DADLTD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.200"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            598..882
                     /locus_tag="JN701_003442"
     CDS             598..882
                     /locus_tag="JN701_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7365767.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            870..1355
                     /locus_tag="JN701_003443"
     CDS             870..1355
                     /locus_tag="JN701_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7365768.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1389..2567
                     /gene="ltrA"
                     /locus_tag="JN701_003444"
     CDS             1389..2567
                     /gene="ltrA"
                     /locus_tag="JN701_003444"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7365769.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2650..2804
                     /locus_tag="JN701_003445"
                     /pseudo
     CDS             2650..2804
                     /locus_tag="JN701_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2858..3340)
                     /locus_tag="JN701_003446"
                     /pseudo
     CDS             complement(2858..3340)
                     /locus_tag="JN701_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3596..4576)
                     /locus_tag="JN701_003447"
     CDS             complement(3596..4576)
                     /locus_tag="JN701_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7365770.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4576..5775)
                     /locus_tag="JN701_003448"
     CDS             complement(4576..5775)
                     /locus_tag="JN701_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY7365771.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(6097..6435)
                     /locus_tag="JN701_003449"
                     /pseudo
     CDS             complement(6097..6435)
                     /locus_tag="JN701_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6502..>7057)
                     /locus_tag="JN701_003450"
     CDS             complement(6502..>7057)
                     /locus_tag="JN701_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7365772.1"
                     /translation="EYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLA
                     IAMGYEAVRAGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLP
                     FSQKEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVV
                     QIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1889 a         1603 c         1687 g         1878 t
ORIGIN      
        1 cggcatccgg catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac
       61 ctgtcgtctc gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc
      121 gatgcgagac acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg
      181 gagcgaaggg ggtcagtcga ggcgtttgag gttaccaatc gaagctatac ccggtaatgg
      241 cgcaggttga cctgcacttg gccaagtgga ttgcccgcaa acacaagcga gcgcgaggta
      301 gtctggtgcg tgcattcgag tggttactgc gaattcgtca cgactgcccg acgctgtttg
      361 cacactggtg cttagcttac gatacttgaa tggttcaacg acaagagccg tatgacgcga
      421 gagtgtcacg tacggttccg tgggagcgta ggggggcggt gcccctgcgc cacccgatat
      481 cccctttgag tgcggttacg tccacagagg taaatgccgc tgacgcggca tctgtaatct
      541 gttactttgt gtacatcatt tgctatatat tgtcaatatg ctattgttgt aaagcaaatg
      601 atatacggag gctttatgaa atcaggtgtt cagctcaatc tcagagcaag agaatcacag
      661 cggatactca ttgatgctgc tgcagaaatt cttcataagt cgcgtacaga tttcattctg
      721 gaaatggcct gtaaagctgc tgaagatgtt attctcgacc ggcgggtgtt taattttaat
      781 gacaggcaat atgaagagtt cattgaaatg cttgatgctc cggttgcaga tgatcctgcc
      841 atcgaaaaat tgctggcaag gaaacctcaa tgggatgtgt aactgcacca gaacctttat
      901 cctcatttca tcaggtcgct gaatttgtca gcggtgaagc tgtacttgat gactggttaa
      961 agcagaaggg gctaaaaaat caggcaatcg gagccacccg gacttttgtt gtatgcagaa
     1021 aaggtacgca acaaatagtc ggtttttact cactggccac tggtagtgtc aatcatacag
     1081 aagctacagg taaccttcgg cgcaatatgc cagaccccat tcctgtaatt atacttgcac
     1141 gcctggctgt agatgtgtca tttcgaggaa aagggcttgg cgcggattta cttcatgatg
     1201 cagttcgtcg ttgttacagg gttgccgaaa atatcggtgt gcgtgcaatt atggttcatg
     1261 cgcttacgga aaacgcaaaa cagttttata ttcatcatgg ttttaagcca tcaaaaactc
     1321 aggtgcaaac attgtttctt aaactccctc aatagggagt aatgcactgg tctgggcatc
     1381 atatctggat gtgcgtcgca acaagggagc cccccggatg cgacgggcag actttgaaaa
     1441 tgttcgacca gcaacgtgat ggcaatctat acaagatctg gaaccggcta tgttcgggaa
     1501 catggtttcc gccaccagtg ctggaaaaac ggatcccgaa gtcgaatggc aaggaacgca
     1561 ttctggggat cccaacagta tccgatcgaa ttgctcaggg agcgataaaa cttttcatgg
     1621 aagaaaagct tgacccgatt ttccatgctg attcatatgg ttaccgacca ggcaaatcag
     1681 ctcatgacgc tctgaaacaa tgtgccatcc ggtgctggcg ttatagctgg atactggaag
     1741 tggacatcag tgcgtttttc gaccatgtga aacatgatct ggtgctcaaa gctctggaac
     1801 accacgggat gcctaaatgg gtcatcctgt actgtcgacg ctggatggaa gcgccaatgc
     1861 agagttgtga aaatggagaa ttaataaccc gaacacgagg cactccgcag ggcggggtca
     1921 ttagtccgct acttgctaac ttgttccttc actatgcatt tgatttgtgg atggaaagag
     1981 aatatcgggg ggtaccgttt gagaggtacg ctgacgatat tgtagtacat tgttcacgaa
     2041 tgagtgatgc aacaaggcta aagaacaggt tatcggagcg cttctcggaa gtcgggctgg
     2101 tcctgaatgc agggaaaacg aatatcgcct acattgacac gtttaaaagg agaaacgtcg
     2161 caacgagttt cacctttctc ggatatgact tcaaagtgcg tacgctgaag aatttcaaag
     2221 gcgaacggta ccgaaaatgc atgccgggtg cgtcaaatgc agcaatgcgc aaaataacag
     2281 aaacaatcaa gaagtggcgt atacatcgct caacagctga gagtttgctg gattttgcga
     2341 ggcgctacaa tacgatagtg agaggctgga tcgagtacta cggaaagttc tggtccagaa
     2401 atttcaacta tcgactgtgg agtgcaatgc agtcacgtct gctcaagtgg atgcagtcta
     2461 aatacagact ttcgaaccgg aaggctcagc gaaagctgac gctggtaagg aaggagtatc
     2521 cgaagctatt tgttcactgg tatttactac gtgcatcaaa tgagtgatca agagccgtat
     2581 gacgcgagag tgtcacgtac ggttccgtga gcagccgggg agtgaaattc tctccggctg
     2641 actcgactcg cgggttccga cagcgatgac gaacatgcgg cggtgttgta ctcgctgatc
     2701 ggcacatgcc gtctgaacaa tgtggaacca gaaaatggct gcgttacgtc attgagcata
     2761 tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctagaaagtt gatctgagct
     2821 ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat tctgctgcgc
     2881 ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt atcagttgtg
     2941 tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ttggacgtcc tgttgctcca
     3001 gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct attgcctgtg
     3061 cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt gccggaagca
     3121 atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat gccccgccag
     3181 cctccatcgc aacagtcatt ttaggctgtt gcaccagcca gttaagcagt tgattgcgag
     3241 aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga taaaagttcg
     3301 ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc ttacttttgt
     3361 ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg ggaagactat
     3421 ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa gaagatacgg
     3481 tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa aagcaaaatt
     3541 tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg aattttcaca
     3601 gcgaattcag attatctttt agtacaagag caatcatacg atctaactta tcctgaactt
     3661 ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt tttcgagcaa
     3721 atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc tttactcctg
     3781 aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt ttaacctcgt
     3841 cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata ttcttcacaa
     3901 cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc agttgcctgt
     3961 aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct ttaggtacgg
     4021 acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt tccgcaattt
     4081 gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt tcacgaatat
     4141 tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa acagtaagct
     4201 catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct gcccgacgac
     4261 gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat ggatagaatt
     4321 gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg gctgtctgtt
     4381 cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca tgatcaacct
     4441 tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga actggagccg
     4501 atatttcttc agtattattc aggatatttg tattcagcgt acggccaata gtcggtcggt
     4561 gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa aaactgcttt
     4621 agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc caacataagt
     4681 aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa aaccatcaag
     4741 acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca ggctatggca
     4801 gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg cctgcaaacg
     4861 gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag ccaaatattt
     4921 aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga tatctgcggc
     4981 agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa ggatgaagtc
     5041 gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt tgttttgtcc
     5101 taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt cgtcaataga
     5161 agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt ccagtaattc
     5221 ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca gccccaccgc
     5281 atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa ggagcagaat
     5341 acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta cggaaacact
     5401 actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat atgcctcact
     5461 ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa tgttctggat
     5521 ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat agccctgagc
     5581 ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa gcttagctac
     5641 agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct tttgctcctg
     5701 aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct tacttaactg
     5761 ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta caaaaaaaac
     5821 tgcattagca gggtaatgca tgcaagtgca tattgtacat gcttatatta aaaatgcgcg
     5881 cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg cctttccggg
     5941 agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg acaacagctt
     6001 tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg acggcgatga
     6061 cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt gcttttcaat
     6121 gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga ttgttttacg
     6181 tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgttcaatac gttgcgtgaa
     6241 gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc cccagtcatc
     6301 gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc gacaggtctc
     6361 atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     6421 ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc tccaccaacg
     6481 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     6541 tttctgtctg agtcgatagc tttctccttt gatttgaacg acatgtgagt ggtgtaagat
     6601 acggcccagc atcgctgagg tcagtgctgc atcaccggcg aacgtttgat cccactgccc
     6661 gaacggtaga ttggatgtca ggatcattgc gctcttttca taacgtttag cgatgacctg
     6721 gaagaacagt tttgcttctt tctgactgaa cggcagatag cctatttcat caatgatgag
     6781 caggcggggg gccattactc cacgctgaag cgtcgtttta taacggccct gacgttgtgc
     6841 cgtagataac tgaagtaaca gatctgctgc tgttgtgaag cgaacttgga tacctgcacg
     6901 gactgcttca tagcccatcg cgattgccag atgggttttc cccacacctg atggccccag
     6961 taatacgata ttttcattac gttctatgaa gctgagtgag cgtaacgact ggagttgctt
     7021 ctgcggtgct ccggtggcga atgtgaagtc atactct
//
LOCUS       DADLTD010000199         7057 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLTD010000199 DADLTD010000000
VERSION     DADLTD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.201"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1293
                     /locus_tag="JN701_003451"
     CDS             <1..1293
                     /locus_tag="JN701_003451"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HAY7365773.1"
                     /translation="GYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDS
                     IAGTGLITKTGSGDLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDC
                     YGLTIGSIDKYQNQAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIE
                     GAGQLTIAQNGSYVLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNG
                     GVLDLSDFSTWQSGTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIRGDGKDGVYVV
                     VDASDGQVSLANNNSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEIT
                     ANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAG
                     TLEPDRQRYNAVGSTCRRRHPERRCCGYLSLCFVTVGR"
     gene            1859..2905
                     /locus_tag="JN701_003452"
                     /pseudo
     CDS             1859..2905
                     /locus_tag="JN701_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244878.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3033..3755)
                     /locus_tag="JN701_003453"
     CDS             complement(3033..3755)
                     /locus_tag="JN701_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY7365774.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3902..6529
                     /gene="gyrA"
                     /locus_tag="JN701_003454"
     CDS             3902..6529
                     /gene="gyrA"
                     /locus_tag="JN701_003454"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY7365775.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6678..>7057
                     /locus_tag="JN701_003455"
     CDS             6678..>7057
                     /locus_tag="JN701_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HAY7365776.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVMHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLD"
BASE COUNT         1693 a         1744 c         2013 g         1607 t
ORIGIN      
        1 ggctatggag atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa
       61 gttacctttg atattgccga cggaaaaacg ctggttatcg gcaatacaga gaatgacgga
      121 gctgttgact ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta
      181 cttaatgcag ataacaatga ctttactggc gagatgcaga ttgaaaacgg tgaagttacc
      241 ctgggccgca gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa
      301 gactgctacg gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac
      361 gttggctcca cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta
      421 aatatcgatg ctggtggcaa tgttactgtt aatcaaggta gttttgctgg caccatcgaa
      481 ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg
      541 atggcgctaa ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac
      601 gcggcagatc tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg
      661 ctcgatctct ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa
      721 gtcagtggca gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc
      781 gacaatttgc atatccgcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc
      841 gacgggcagg taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc
      901 ggtacgctga tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt
      961 atctttaccg ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc
     1021 tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt
     1081 gatgcggggg tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag
     1141 gatgagggta gcacatttac taaaacgggg gcgggtacgc tggagcctga ccgccagcgg
     1201 tacaacgcag tcggcagtac gtgtcgaaga aggcaccctg aaaggcgatg ttgcggatat
     1261 ctttccttat gcttcgtcac tgtgggtcgg tgatggggcg acgttcgtta ctggcgcgga
     1321 tcaggatatt cagtctatcg atgctacttc cagcggcact atcgatatca gcgatggtac
     1381 ggttttgcgt ctgaccgggc aggatacttc cgtcgccctt aatgcctcat tgtttaacgg
     1441 cgatgggacg ctggtgaatg ctaccgatgg cgtgacgttg acaggtgagc ttaataccaa
     1501 ccttgaaact gacagcctga cctatcttgc cgacgtgacg gttaatggtg atctgaccaa
     1561 tacgtccggt gcggttagcc tgcaaaatgg cgtcgctggc gatacgctaa cggtaaacgg
     1621 tgattatact ggcggcggta cgctactgtt cgacagcgaa ttaaacggcg atgactcggt
     1681 aagcgatcaa ttggtgatga acggtaatac tgctggcaac acaactgtgg tggttaactc
     1741 cattacaggg attggtgagc cgacatcgac aggcattaaa gtggttgatt tcgcagctga
     1801 tcccacgcag tttcaaaaca atgcacagtt cagtctggct ggcagtggct acgtcaacat
     1861 gggagcgtat gactacacgc tagtggaaga taacaacgac tggtatctgc gatcgcaaga
     1921 agtaacgccg ccatcgccac ctgatccaga cccggaccca acacccgatc ctgacccaac
     1981 acccgatcca atccccgctt accagccggt gctgaatgcc aaagttggcg gttatctcaa
     2041 taacctgcgg gcggcaaatc aggcgtttat gatggagcga cgcgatcacg ctggtggcga
     2101 tggtcagacg ctgaatttac gtgttatcgg cggacgttat cattacacgg cggcagggca
     2161 actggctcag caggaagaca cttctacggt gcagcttagc ggcggcttgt ttagcgggcg
     2221 ctggggcacg gatggcgagt ggatgcttgg ggcggttggt ggctacagtg ataatcaggg
     2281 cgacagccgc tcgaatatga ccggaactcg cgccgataac cagaaccacg gttatgccgt
     2341 tgggctgacc tcaagctggt atcagcacgg taattagaag cagggggcct ggctggatag
     2401 ctggctgcaa tacgcgtggt ttaacaatga tgtttccgaa catgaagatg gcgtggatcg
     2461 ttaccattcg tcggggatta tcgcctcgct ggaagccggg tatcagtggt taccggggcg
     2521 tggtgtggtg attgaaccgc aggcgcaggt gatttatcag gacgtgcagc aggatgattt
     2581 taccgccgct aaccatgcgc gcgtgtcaca atcgcagggg gatgatattc agacgcggct
     2641 gggtttacac agcgaatggc gtaccgcggt tggtgtcaca ccaacattag atctgaatta
     2701 ttatcacgat ccccatgcga cggaaattga agaagatggc agcactatca gtgacgatgc
     2761 ggtgaagcga cggggtgaaa taaaagtggg agtcacgggc aatatcagtc agcgagtgtc
     2821 cctgcgcggc agcgtggcgt ggcagaaagg gagtgatgat tttgcccaga cggcagggtt
     2881 tttgtcgatg acggtgaaat ggtaatcact gtctagtcgg cacggttgcc ggatgcggcg
     2941 taaacgcctt atccggccta cggggagcat ttgtaggcct gataagacgc ggcaagcgtc
     3001 gcatcaggca ttggctcggc caaacaacaa cctcacttat tctgcgtgtg cagcatatag
     3061 ttcacatcca cgccggggcc gagtttaaaa gtattagtga gcgggttgta atgtagccca
     3121 gttatatgtc gctctttaag gcttgtctga tctacccagc caagcagttc tgccggttta
     3181 ataaacttct tcacatcatg cgtgcctttg ggcaccatgc gcaaaatata ttccgcgcca
     3241 accaccgcca tcagccatga cttgccgttg cggttaagcg tcgagaaaaa gacatcgccg
     3301 cctggtttca ccagttgcgc acaggctctg accactgact gcggatcggg gacgtgctcc
     3361 agcatctcca tgcaggtcac cacatcatac tgcccggcat gttttgccgc gtgctcttcc
     3421 acggtttcct gcacataatc cacctgaatg ccgctttcca gcgcgtgcag ttttgccacc
     3481 tgcaatggct caaagcccat atccagaccg gtcaccgtcg cgccttcgcg cgccatactc
     3541 tcggccagaa tgccgccgcc acaaccgaca tcgagcacct ttttgccaaa taaaccgcca
     3601 gcacgctcgg caatatagcc cagacgcagc gggttaatgc ggtgcaacgg tttgaactca
     3661 ccttcaagat cccaccagcg ggaggcgacg gcttcaaatt tagcgatctc ttcgtggtct
     3721 acgttatggt tttccggcga tttttcggca ttcattggca cttctactcc gtaattggca
     3781 agacaaacga gtatatcagg cattggatgt gaataaagcg tataggttta cctcaaactg
     3841 cgcggctgtg ttataatttg cgacctttga atccgggata cagtagaggg atagcggtta
     3901 gatgagcgac cttgcgagag aaattacacc ggtcaacatt gaggaagagc tgaagagctc
     3961 ctatctggat tatgcgatgt cggtcattgt tggccgtgcg ctgccagatg tccgagatgg
     4021 cctgaagccg gtacaccgtc gcgtacttta cgccatgaac gtactaggca atgactggaa
     4081 caaagcctat aaaaaatctg cccgtgtcgt tggtgacgta atcggtaaat accatcccca
     4141 tggtgactcg gcggtttatg acacgatcgt ccgtatggcg cagccattct cgctgcgtta
     4201 catgctggta gacggtcagg gtaacttcgg ttccatcgac ggcgactctg cggcggcaat
     4261 gcgttatacg gaaatccgtc tggcgaaaat tgcccatgaa ctgatggccg atctcgaaaa
     4321 agagacggtc gatttcgttg ataactatga cggcacggaa aaaattcccg acgtcatgcc
     4381 aaccaaaatt cctaacctgc tggtgaacgg ttcttccggt atcgccgtag gtatggcaac
     4441 caacatcccg ccgcacaacc tgacggaagt catcaacggt tgtctggcgt atatcgatga
     4501 tgaagacatc agcattgaag ggctgatgga acacatcccg gggccggact tcccgacggc
     4561 ggcaatcatt aacggtcgtc gcggtattga agaagcttac cgtaccggtc gcggcaaggt
     4621 gtatatccgc gctcgcgcag aagtggaagt tgacgccaaa accggtcgtg aaaccattat
     4681 cgtccacgaa attccgtatc aggtaaacaa agcgcgcctg atcgagaaga ttgcggaact
     4741 ggtaaaagaa aaacgcgtgg aaggcatcag cgcgctgcgt gacgagtctg acaaagacgg
     4801 tatgcgcatc gtgattgaag tgaaacgcga tgcggtcggt gaagttgtgc tcaacaacct
     4861 ctactcccag acccagttgc aggtttcttt cggtatcaac atggtggcat tgcaccatgg
     4921 tcagccgaag atcatgaacc tgaaagacat catcgcggcg tttgttcgtc accgccgtga
     4981 agtggtgacc cgtcgtacta ttttcgaact gcgtaaagct cgcgatcgtg ctcatatcct
     5041 tgaagcatta gccgtggcgc tggcgaacat cgacccgatc atcgaactga tccgtcatgc
     5101 gccgacgcct gcagaagcga aaactgcgct ggttgctaat ccgtggcagc tgggcaacgt
     5161 tgccgcgatg ctcgaacgtg ctggcgacga tgctgcgcgt ccggaatggc tggagccaga
     5221 gttcggcgtg cgtgatggtc tgtactacct gaccgaacag caagctcagg cgattctgga
     5281 tctgcgtttg cagaaactga ccggccttga gcacgaaaaa ctgctcgacg aatacaaaga
     5341 gctgctggat cagatcgcgg aactgttgcg tattcttggt agcgccgatc gtttgatgga
     5401 agtgatccgt gaagagctgg agctggttcg tgaacagttc ggtgacaaac gtcgtactga
     5461 aatcaccgcc aacagcgcag acatcaacct ggaagatctg atcactcagg aagatgtggt
     5521 agtgacgctc tctcaccagg gctacgttaa gtatcagccg ctttctgaat acgaagcgca
     5581 gcgtcgtggc gggaaaggta aatctgccgc acgtattaaa gaagaagact ttatcgaccg
     5641 actgctggtg gcgaacactc acgaccatat tctgtgcttc tccagccgtg gtcgcgtcta
     5701 ttcgatgaaa gtttatcagt tgccggaagc cactcgtggc gcgcgcggtc gtccgatcgt
     5761 caacctgctg ccgctggagc aggacgaacg tatcactgcg atcctgccag tgaccgagtt
     5821 tgaagaaggc gtgaaagtct tcatggcgac cgctaacggt accgtgaaga aaactgtcct
     5881 caccgagttc aaccgtctgc gtaccgccgg taaagtggcg atcaaactgg ttgacggcga
     5941 tgagctgatc ggcgttgacc tgaccagcgg cgaagacgaa gtaatgctgt tctccgctga
     6001 aggtaaagtg gtgcgcttta aagagtcttc tgtccgtgcg atgggctgca acaccaccgg
     6061 tgttcgcggt attcgcttag gtgaaggcga taaagtcgtc tctctgatcg tgcctcgtgg
     6121 cgatggcgca atcctcaccg caacacaaaa cggttacggt aaacgtaccg cagtggcgga
     6181 atacccaacc aagtcgcgtg cgacgaaagg ggttatctcc atcaaggtta ccgaacgtaa
     6241 cggtttagtt gttggcgcgg tacaggtaga tgactgcgac cagatcatga tgatcaccga
     6301 tgccggtacg ctggtacgta ctcgcgtttc agaaatcagc atcgtgggcc gtaacaccca
     6361 gggcgtgatc ctcatccgta ctgcggaaga tgaaaacgta gtgggtctgc aacgtgttgc
     6421 tgaaccggtt gacgaggaag atctcgatac catcgacggc agtgccgcgg aaggggacga
     6481 tgaaatcgct ccggaagtgg acgttgacga cgagccagaa gaagaataat tttacttctt
     6541 catgccaaaa gggagctatc tcccttgttt gaattgaaaa gtccaggctg caaagtctgg
     6601 gcttttgtcg tattagggca cggtaaagtt tggctgtgcc cgtaaaaaat ggctggctat
     6661 acacaaggaa tgtggcaatg agtggtgaaa aaaaggcgaa aggctggcgg ttctatggtc
     6721 ttgtaggttt tggcgcaata gtactgcttt ccgctggcgt ctgggcgttg caatatgctg
     6781 gcagtgggcc agaaaaaacg ttgtcgccgc tggtgatgca caacaatctg caaatcgatc
     6841 tcaatgagcc ggacctcttt ctcgacagcg actctctgag ccagcttccc aaagatctcc
     6901 tcaccattcc gtttctccac gatgttctga gtgaagattt cgttttctat tatcagaatc
     6961 atgccgatcg tctgggcatt gaaggcagca ttcgtcgcat tgtctatgaa cacgatctca
     7021 cgctgaaaga taaactcttt tcgtcactct tagatca
//
LOCUS       DADLTD010000200         6998 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLTD010000200 DADLTD010000000
VERSION     DADLTD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.202"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..353
                     /locus_tag="JN701_003456"
     CDS             <1..353
                     /locus_tag="JN701_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365777.1"
                     /translation="PANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWV
                     CGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALV
                     ELAVLVVIIVSRGA"
     gene            356..1279
                     /gene="hycD"
                     /locus_tag="JN701_003457"
     CDS             356..1279
                     /gene="hycD"
                     /locus_tag="JN701_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY7365778.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            1297..3006
                     /locus_tag="JN701_003458"
     CDS             1297..3006
                     /locus_tag="JN701_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY7365779.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAELLTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            3016..3558
                     /gene="hycF"
                     /locus_tag="JN701_003459"
     CDS             3016..3558
                     /gene="hycF"
                     /locus_tag="JN701_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY7365780.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            3558..4325
                     /gene="hycG"
                     /locus_tag="JN701_003460"
     CDS             3558..4325
                     /gene="hycG"
                     /locus_tag="JN701_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY7365781.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            4322..4732
                     /locus_tag="JN701_003461"
     CDS             4322..4732
                     /locus_tag="JN701_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY7365782.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            4725..5195
                     /gene="hycI"
                     /locus_tag="JN701_003462"
     CDS             4725..5195
                     /gene="hycI"
                     /locus_tag="JN701_003462"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY7365783.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(5354..6778)
                     /locus_tag="JN701_003463"
     CDS             complement(5354..6778)
                     /locus_tag="JN701_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7365784.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(6787..>6998)
                     /locus_tag="JN701_003464"
     CDS             complement(6787..>6998)
                     /locus_tag="JN701_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7365785.1"
                     /translation="GLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIP
                     VEEAAAQARKHQSVQPTVAKEVSLN"
BASE COUNT         1507 a         1898 c         1975 g         1618 t
ORIGIN      
        1 agcctgctaa caccaccgtt tctcaaccaa tgatcacatt gctgctgatt gcctgcccgc
       61 tgctgccatt catcattatg gcgatttgca aaggcgaccg cctgccgtca cgttcccgcg
      121 gtgcggcctg ggtgtgcggc tacgaccatg aaaaatcaat ggtgattacc gctcacggtt
      181 ttgccatgcc ggttaaacag gcgtttgcgc cagtgctgaa actgcgcaaa tggctgaatc
      241 cggtgtcgct ggtgccgggc tggcagtgcg aaggtagtgc gttgctgttc cgccggatgg
      301 cgctggttga actggcggta ctggtggtga ttattgtttc acgaggagcc tgagaatgag
      361 tgttttatat ccgttaattc aggcgctggt gttatttgcc gttgcgccgc tgctctccgg
      421 tattacccgc gtggcgcgcg cccgtctgca taaccgtcgc gggccgggcg tgttgcagga
      481 gtatcgcgac attatcaaac tgctgggccg ccagagcgtt ggcccggatg cctctggctg
      541 ggtgttccgc ctgacgccgt atgtgatggt gggcgtcatg ctgactatcg ctactgcgct
      601 gccggtggtg accgtcggtt ctccgctgcc gcaactgggc gatttgatca ccttactgta
      661 tctcttcgcc atcgcgcgtt tcttctttgc catttccggt ctggataccg gtagcccgtt
      721 taccgctatc ggtgcgagcc gtgaagcgat gcttggcgtg ctggtcgaac cgatgttgct
      781 gcttggtctg tgggttgccg cacaggttgc cggttccacc aacatcagca acattaccga
      841 caccgtttat cactggccgc tgagccaaag catcccgctg gtgctggcgc tctgcgcctg
      901 tgcgttcgcc acttttatcg aaatgggcaa actgccgttc gacctggcgg aagccgagca
      961 ggagttgcag gaaggtccgc tctctgagta tagcggcagc ggctttggcg tcatgaaatg
     1021 gggtatcagc ctgaaacagc tggtggtgtt gcagatgttc gtcggggtgt ttattccgtg
     1081 gggacaaatg gaaaccttca ccgccggtgg actgctgctg gcgctggtga ttgcgatcgt
     1141 aaaactggtg gtcggcgtcc tggttatcgc gctgttcgaa aacagcatgg cccgtctgcg
     1201 tcttgatatt actccgcgca ttacctgggc tgggtttggc tttgcatttt tagcgttcgt
     1261 ctccttgctg gcggcgtgat taaagagagt ttgagcatgt ctgaagaaaa attaggtcaa
     1321 cattatctcg ccgcgctgaa tgaggcattt ccgggcgtcg tgctggacca cgcctggcag
     1381 accaaagatc agctgactgt caccgtaaag gtgaactatc tgccggaagt ggtggagttt
     1441 ctctactaca agcagggggg ctggctgtcg gtgctttttg gtaacgacga acgcaaactg
     1501 aatggtcatt acgccgttta ttacgtgctg tcgatggaga aggggactaa gtgctgggta
     1561 acggttcgcg tcgaagttga tgccaacaaa ccggagtatc cgtccgtgac gccgcgcgtt
     1621 ccggcggcgg tgtggggcga gcgcgaagtg cgcgatatgt acggtttgat tccggttggt
     1681 ctgccggatg aacgccgtct ggtgctgccg gatgactggc cggatgaact ttatccgctg
     1741 cgtaaagaca gcatggatta tcgtcagcgt ccggcaccga ccaccgatgc tgaaacctac
     1801 gagttcatca acgaactggg cgacaagaaa aacaacgtcg tgccgattgg tccgctgcac
     1861 gtcacttctg acgaaccggg ccacttccgt ctgttcgtcg atggcgaaaa cattatcgac
     1921 gcagactacc gcttgttcta tgtccatcgc ggtatggaaa aactggcgga aacccgcatg
     1981 ggttataacg aagtgacctt cctctctgac cgtgtgtgcg ggatctgcgg cttcgcccac
     2041 agcaccgcct acaccacgtc ggtggaaaac gcgatgggta ttcaggtgcc agaacgtgcg
     2101 cagatgatcc gcgccattct gctggaggtg gaacgcctgc actcgcatct gctcaacctc
     2161 ggcctcgcct gccactttac cggcttcgac tccggcttta tgcaattctt ccgcgtgcgt
     2221 gaaacctcca tgaaaatggc cgagctcctc accggtgcgc gtaaaaccta cggcctgaac
     2281 ctgatcggcg ggattcgtcg cgatctgttg aaagatgaca tgatccagac ccgccagctg
     2341 gcgcaacaga tgcgtcgtga agtgcaggag ctggtggatg tgctgctgag cacaccgaac
     2401 atggaacagc gcaccgtcgg cattggtcgt ctggacccgg aaattgcccg cgatttcagt
     2461 aacgttggcc cgatggtccg cgccagcgga cacgctcgcg atacccgcgc tgatcacccg
     2521 tttgttggtt atggcctgct gccaatggaa gtccacagcg agcagggctg cgacgttatt
     2581 tcgcgtctga aagtgcgtat caacgaagtc tataccgcgc tgaacatgat cgactacggt
     2641 ctggataacc tgccgggtgg cccgctgatg gtggaaggct ttacctacat tccgcaccgt
     2701 ttcgcgctgg gctttgccga agcgccgcgc ggtgatgata tccactggag catgactggc
     2761 gacaaccaga agctgtaccg ctggcgctgc cgtgccgcga cctacgcgaa ctggccgacc
     2821 ctgcgctaca tgctgcgtgg caacactgtt tccgatgcgc cgctgattat cggtagcctg
     2881 gacccttgct actcctgtac cgaccgcatg actgtggtcg atgtacgtaa gaagaagagc
     2941 aaagtggtgc cgtacaaaga actcgagcgc tacagcattg agcgtaaaaa ctcgccgctg
     3001 aaataaggaa tcgccatgtt tacctttatc aaaaaagtta tcaaaaccgg tacggcgacc
     3061 tcgtcttatc cgctggagcc gattgcggtt gataaaaact tccgtggtaa gccagagcag
     3121 aacccgcagc agtgcatcgg ctgcgcggcc tgcgtcaatg cctgcccgtc aaacgcctta
     3181 acggttgaaa ctgacctcgc cacaggagag ctggcctggg agtttaatct cgggcgctgc
     3241 atcttctgtg gacgctgcga agaagtttgc ccgacggcgg cgattaagct gtcgcaagag
     3301 tacgaactgg cggtgtggaa gaaagaagac ttcctgcaac agtctcgctt cgcgctgtgc
     3361 aactgtcgcg tctgcaatcg tcctttcgcc gtccagaaag agatcgacta cgccattgcg
     3421 ctgcttaagc acaacggcga cagccgcgcg gaaaaccacc gcgaaagctt tgagacttgc
     3481 ccggaatgta agcgccagaa atgcctggtg ccgtccgacc gtattgaact gactcgccat
     3541 atgaaagagg ccatctgatg agcaatttat taggcccccg tgacgccaac ggcattccgg
     3601 tcccgatgac ggtggatgaa tccatcgcca gcatgaaggc gtcgttactg aaaaaaatca
     3661 aacgttctgc ctatgtttac cgcgtggact gcggcggctg caacggctgc gaaatcgaaa
     3721 ttttcgccac tttatcgccg ctgtttgatg cagaacgctt cggcattaaa gtcgttcctt
     3781 caccgcgtca tgcggatatt ttactgttta ccggcgcggt cacccgtgca atgcgatccc
     3841 ctgcgctgcg tgcgtggcag tccgcgccgg acccgaaaat ttgtatctcc tacggtgcct
     3901 gcggtaacag cggcgggatc ttccacgatc tctactgcgt gtggggcggt acggataaaa
     3961 ttgtccctgt ggatgtttat atccctggct gcccgccaac gcctgccgcc acgctgtacg
     4021 gctttgcaat ggcgctcggc ctgctggagc agaaaattca cgcccgtggg ccgggtgaac
     4081 tggatgaaca accggcggag atcctgcatg gcgatatggt gcagccgctg cgcgtgaaag
     4141 tggatcgcga agcacgccgc ctggcgggtt atcgttacgg tcgtcagatt gccgatgact
     4201 tcctgacaca gttagggcag ggcgaagaac aggttgcacg ctggctggaa gcggaaaacg
     4261 atccgcgtct gaacgagatt gtcagccatc tgaatcatgt tgtagaagag gtgcgtatcc
     4321 gatgagtgaa aaggtggtgt tcagtcaact gagccgtaaa tttattgatg agaacgatgc
     4381 cacgcccgcc gaggcgcagc aggtggtcta ttacagcctg gcgattggtc accaccttgg
     4441 ggttatcgat tgcctggaag cggcgctcac ctgcccgtgg gatgaatatc tggcatggat
     4501 tgccactctg gaggcgggca gtgaagcccg ccgcaaaatg gaaggcgtac cgaagtacgg
     4561 tgagatcgtc atcgacatta accatgtgcc gatgctggcc aacgcattcg ataaagcccg
     4621 ggcagcgcaa acttcgcagc agcaggaatg gagtacaatg ctgttaagta tgctgcatga
     4681 tattcatcag gaaaacgcca tctatttgat ggtgaggaga ttgcgtgact gacgttttac
     4741 tctgtgttgg caatagcatg atgggcgatg atggcgcagg tccgctgctg gcggaaaagt
     4801 gcgccgccgc gccgaaaggt aactgggtgg tgattgacgg cggtagtgca ccggaaaacg
     4861 acatcgtcgc catccgtgaa ctgcgcccta cgcggttact gattgtcgac gccacggata
     4921 tggggctaaa ccccggcgag atccgcatca tcgacccgga tgatatcgcc gagatgttta
     4981 tgatgactac ccataacatg cctctgaact atcttatcga ccagttgaaa gaagatattg
     5041 gcgaagtgat tttcctcggc atacagccgg atatcgtcgg cttttactac ccgatgaccc
     5101 agccgattaa agatgcggta gaaaccgttt atcaacgact ggaaggctgg gaagggaatg
     5161 gcggcttcgc gcagttagcg gtggaagaag agtagttttt cattaaggaa tcaggacagg
     5221 gatgttcttg atggggtgaa ccagctctga tgccaaatgc taaattgccc gatgcgctgc
     5281 gcttatcggg ccttcctggt tcgtgcgaca tgtaggccgg ataaggcgtt cacgccgcat
     5341 ccggcactgt tacttactct aaatcttccc cattactggc aatcactttt ttataccacc
     5401 agaacgattt cttacgtgtg cgcgtcagcg tgccgttgcc tgcgtcgtca cggtctacgt
     5461 agacaaaacc gtagcgtttg ctcatttcac ccgtagaggc ggaaactaaa tcaatacagc
     5521 cccatgtggt gtagcccatc agcggaatgc catctgcaat cgcttcgccc attgcgcgga
     5581 tatgttcgcg taaatagctg atgcgatagt catcgttaat ctcgccattg gcagcaagtt
     5641 catcttttgc gccgaggccg ttttccacca gaaacagcgg cttctgataa cgatcgtaca
     5701 tcatattcat ggtgatacgc agaccgagtg gatcaattcc ccagccccag tcgctcactt
     5761 gcagatacgg gttacgcagc gatttcacca cgttcgccgc actgctgttg ttggcgttca
     5821 tctccgccga ggcgcagcgc gaggcgtaat agctgaaaga gacaaaatcg acggtgtttt
     5881 tcaggatctc atcatcgccc agcgctttat ctatggttac ccctttttcg cgaaatacgc
     5941 gggcagagta agccggatac gcgccgcgcg cctgcacatc gataaaaaac aggttttccc
     6001 gatctttctc cagcgccgcc cagacatctt ccggtttgca gctgtaaggg tagaagttgc
     6061 cgcccgccag catacagccc acctgatttt gcgggttaac ctcatgggcg attttggcgg
     6121 ctagcgcact ggcaaccagc tggtgatgcg cggcctgata tttcacctga tcctgatttt
     6181 caccttcttc aaacaccaga cccgcgccgg agaacgggct atgcaacata atgttgattt
     6241 cattgaaggt tagccagtat ttcaccagac catcaaatgc ttcaaagcag gttctggcgt
     6301 agcggctgaa aaactccacc agcttgcggt tacgccagga gccatattcg gtgaccagat
     6361 gcatcggcac atcgaagtgg cacaacgtga ccagcggttc gataccgtac tttttacact
     6421 cttcaaagac agaacgataa aaagcaatgc cctgctgatt gggcgtgatt tcatcgccct
     6481 gcggaaagag acggctccag gcaattgagg tacggaaaac cttgaatccc atctctgcca
     6541 tcagggcgat atcttcttta taacgatgat aaaaatccgt cgcctcatgg ctgggataaa
     6601 actcgtcatc gcgcaactga aaacgttttt ccagccccag tttcaccgcc attcgatgct
     6661 cgccgtgtgg gatcatatcg acggtggtca gcccttttcc gccttcacgg aacgcacctt
     6721 cagactggtt ggcggcaagc gcgccgcccc ataaaaaacc ttctggaaat actgacattt
     6781 tcatcctcaa ttaagactta cttctttggc gacggtcggt tgtacgctct gatgctttcg
     6841 cgcctgggca gccgcttcct caacaggaat atcctcaaag ccgagcaaca gtgtgaggat
     6901 aaacgacagc accaccgcca gcgccatgac cgcgaacacc cagacgatgc tcattggatt
     6961 cgccggatcg aagaattgca cgctggtaaa tagccccg
//
LOCUS       DADLTD010000201         6964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLTD010000201 DADLTD010000000
VERSION     DADLTD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.203"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..4867
                     /gene="traI"
                     /locus_tag="JN701_003465"
     CDS             <1..4867
                     /gene="traI"
                     /locus_tag="JN701_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY7365786.1"
                     /translation="TGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSSDK
                     VGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGR
                     SQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQMLKETGFDIRAYRDAA
                     EQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPENGV
                     IERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHPEK
                     SVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAAD
                     RRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAAR
                     HNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARLAG
                     DFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRS
                     RYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLDSS
                     WSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLP
                     VSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSS
                     LDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALPVL
                     ESKNLAFSMVDLLTEAKSFTAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLLVS
                     RASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTVVQ
                     GYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHD
                     TQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKSEAFPDVPMTLYAAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            4887..5633
                     /gene="traX"
                     /locus_tag="JN701_003466"
     CDS             4887..5633
                     /gene="traX"
                     /locus_tag="JN701_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7365787.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            5688..6248
                     /locus_tag="JN701_003467"
     CDS             5688..6248
                     /locus_tag="JN701_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7365788.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            6379..6591
                     /locus_tag="JN701_003468"
     CDS             6379..6591
                     /locus_tag="JN701_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7365789.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            6775..>6964
                     /locus_tag="JN701_003469"
     CDS             6775..>6964
                     /locus_tag="JN701_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAY7365790.1"
                     /translation="MLHYSGGLKYRCHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAV"
BASE COUNT         1593 a         1763 c         2285 g         1323 t
ORIGIN      
        1 gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc aggaaccaca
       61 gttacacacg catgcggtgg tgactaatgt tacgcagcat aacggcgaat ggaagacact
      121 gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta atcagattgc
      181 ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg gctatgaaac
      241 tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg aggccttttc
      301 cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga aatcccggga
      361 tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag tcagaatggc
      421 cgagtggatg cagatgctga aggaaaccgg gttcgacatc cgggcatatc gtgacgcagc
      481 ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg acgggccgga
      541 tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag tgcagtttat
      601 gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg gtgtgattga
      661 acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc ccctcgatcg
      721 tgagaagggg ctgttcacgt ccgggattca tatgctcgat gagctgtcag tccgggcact
      781 cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa gtgtcccccg
      841 gacggccggt tacagctatg ccgtcagtgt tctggcacag gaccgtccgt cgctggccat
      901 tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac tggtcatgat
      961 ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct cgcagatgaa
     1021 cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc agttgctgga
     1081 aggcatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa aactctccct
     1141 gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg tcctgataac
     1201 tgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg atgccggggt
     1261 gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca gtgaaccgga
     1321 ccgttatgtc cgctatgccc ggctggccgg agattttgcg gccagcgtga aagccggaga
     1381 agagagcgtg gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg
     1441 cagtgagctg aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc
     1501 accggtctgg ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt
     1561 gatggagcag tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac
     1621 ggcgcagagt cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat
     1681 ttcttccctg gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg
     1741 tgagcgtctg agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct
     1801 gcaggtggca tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc
     1861 ggccaccctg cctgtgagcg attcaccgtt cacggcactg aagctggaga acggctgggt
     1921 ggaaacgccc gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat
     1981 ggcaatggac aatgccaccc tgaacggtct ggcccgcagt ggtcgtgatg tccggctgta
     2041 ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc cgccatcctt cctttacggt
     2101 ggtttctgag cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct
     2161 gcagaaagcc gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct
     2221 ggaaagtaaa aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttac
     2281 tgcagaagga accggttttg ctgacctggg aggggaaatc aatgcgcaga taaaacgggg
     2341 tgatttactg tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc
     2401 gtcgtatgag gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac
     2461 gccgctgatg gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc
     2521 cgccgcccgt atgatactgg aaacgtccga ccgtttcacg gtggtgcagg gctatgccgg
     2581 tgtgggtaag accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag
     2641 tgaacgtccc cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag
     2701 cgccggcgtg gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg
     2761 cagcggagaa acgccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt
     2821 gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt
     2881 ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca
     2941 gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga
     3001 actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct
     3061 tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc
     3121 cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat
     3181 gctgaaaagc gaggcttttc cggatgtccc catgacactg tatgcagcca ttgtccgcga
     3241 ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga
     3301 ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg
     3361 gcaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg
     3421 tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca
     3481 ccggattgcc ggtatcagca aggatgacgg gctgataacc ctgcaggatg cggaaggtaa
     3541 cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga
     3601 caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg
     3661 ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc
     3721 tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga
     3781 cctggcctat gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc
     3841 gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc
     3901 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     3961 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     4021 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     4081 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     4141 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     4201 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     4261 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     4321 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     4381 ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa
     4441 tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     4501 gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     4561 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     4621 caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga
     4681 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga
     4741 accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg
     4801 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     4861 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     4921 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     4981 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     5041 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     5101 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     5161 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccctggt
     5221 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     5281 cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt
     5341 ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc
     5401 gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg
     5461 cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga
     5521 ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt
     5581 tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt
     5641 ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga
     5701 agcgaccggt actgacactg aagcggaaga cagagggaac agcgactgtc cgcagccgga
     5761 aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg
     5821 agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
     5881 ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc
     5941 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
     6001 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
     6061 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
     6121 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc
     6181 gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
     6241 agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat
     6301 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     6361 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     6421 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     6481 gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt
     6541 gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggggta acgaaaaaag
     6601 cagccttcat tactccagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa
     6661 tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa
     6721 gtcgattttt tatattttgt gattaatttc acaaaataag gtgttattca atgtgtgctg
     6781 cactattcag gggggctgaa atacaggtgc catttatctg atatggagaa taacatgagg
     6841 aaatatattc cactggtatt atttatcttt tcatggccgg tattgtctgc agatattcat
     6901 ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg
     6961 gtgc
//
LOCUS       DADLTD010000202         6854 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLTD010000202 DADLTD010000000
VERSION     DADLTD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.204"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            165..389
                     /locus_tag="JN701_003470"
     CDS             165..389
                     /locus_tag="JN701_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365791.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            641..847
                     /locus_tag="JN701_003471"
     CDS             641..847
                     /locus_tag="JN701_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365792.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            847..1902
                     /locus_tag="JN701_003472"
     CDS             847..1902
                     /locus_tag="JN701_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAY7365793.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            1914..2249
                     /locus_tag="JN701_003473"
     CDS             1914..2249
                     /locus_tag="JN701_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAY7365794.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            2262..2675
                     /locus_tag="JN701_003474"
     CDS             2262..2675
                     /locus_tag="JN701_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAY7365795.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            2837..3271
                     /locus_tag="JN701_003475"
     CDS             2837..3271
                     /locus_tag="JN701_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365796.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMASFLAGWRHAVSVRRASMEQSADTEQ"
     gene            3552..3833
                     /locus_tag="JN701_003476"
     CDS             3552..3833
                     /locus_tag="JN701_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365797.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(4498..>6854)
                     /gene="yejO"
                     /locus_tag="JN701_003477"
     CDS             complement(4498..>6854)
                     /gene="yejO"
                     /locus_tag="JN701_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY7365798.1"
                     /translation="GGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVYG
                     AANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVME
                     AFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATGV
                     DKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTILD
                     EHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGTM
                     VNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSVW
                     AIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGNG
                     NFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTLG
                     NAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTVR
                     ERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTIR
                     GLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKMS
                     NGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADVG
                     NTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAGT
                     PGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT         1857 a         1671 c         1618 g         1708 t
ORIGIN      
        1 gctggtgatg aaatatggcg caattaccag gacagctaat caaacagccg gagaaatccg
       61 gcttttttgc accagttgaa acggtatggc gcattaccgg gttttcgtca cggtcaggca
      121 tagttactat ctgaaacaaa cagacacaac agaggaaaaa aacaatgccg atgaaatttg
      181 atgagatatt aaaacagcgt gataaatacc atgctgacaa catggagacg atgagcatca
      241 atgattaccg cgcattcctg gagacgggcg cactgattga aaaggatcag catggttttg
      301 tgagatgtgc tctatccggt gaaatgctgg cggtaaatcc tgaacagata gatgcattga
      361 tagaatttct gaaagagatc agagactgat ccagcacaca gcacacatag ccggagcaat
      421 ccggcttttt tgcgcccaaa aaaagcccga taaggtcaga gggttcttat cgggcttttg
      481 catatgaggt ttttggatgc actggcgttc gtgatcggga taatcatttc ataatttgca
      541 acataactca atattattgc ataaaatgca attctgatta taatcaggac tggataaaca
      601 tccagttatg atttttttag tcgaagagga atttcttact atggctgaag agaaaaaagg
      661 cggtgtttcg gtgtacataa gccccgaaat cgtggaggtg ctcaagcagc gccacaaaaa
      721 aaactatgag gctggcgtgg cggctggact ggatccgctg atgacgccgg agccgtcgat
      781 aggttcactt gtacgctctt atttacttgc ggcgcttggg atgcataaaa attatggggg
      841 tgaataatgg caggcaaagc aacggcactt aacactaacc agctttttat gtacctgaat
      901 cgcggggata ttgcggattt taaattcagc cctctgttta ccacgctgtt tttcccgaac
      961 gtggcgacat tcagcacgca aaacatcatg ctggataccc tggacattga agaagtcacc
     1021 atgtcggcgt tttgttcgcc tatggtgggt agccaggttc agcgcgataa agggtacgaa
     1081 accagcacaa tcaaacctgg ctacatgaag ccaaagcacg aaatcgatcc aacgaaaacc
     1141 atcatgcgca tggctggaga agatccggca cagcttaacg accctaccta tcgccgtatg
     1201 cgcctgatta ctggcaacat gcgccgccag ataaacgcca ttaaggcacg cgtggaatgg
     1261 ctggcggtga atgcggtaac gaccggaaaa aacatcattg agggcgaagg catagaacgc
     1321 tatgaaatcg actggaagat accggaaaaa aacatcatag agcagtccga cggcaaaaaa
     1381 tggtccgagc atgataaaga tatttacgat ccaatctatg acatcgaact atacgcagat
     1441 caggcaggtt gccccgccaa cgtcatgatt atgggcgctg aggtatggcg cacgttacgc
     1501 agctttaaaa aattccgtga actgtacgat ctttcccgtg gttcagaatc cgccgcagaa
     1561 atggcctgta aaaacctggg cgaagtggtg agctttaaag gctatctggg cgatattgct
     1621 cttattgtct attccggcaa atacactgac agcgacggca ccgaaaaata tttccttgag
     1681 cctgatttgc tggtcctggg caacaccaac aataaagggc tggtggccta tggtgcgatt
     1741 atggatcagg aagcggtaag aacgggcgca acgcaaaaca tgtactaccc gaaaaactgg
     1801 attgaggacg gcgatccggc gattgagtac gtgcagacac acagcgcacc gcagccggtt
     1861 ccggcagata ttcgcaaatt tgttaccgtc aaaattggtt aacgggggat tatatggaca
     1921 ctccatatat tgagttattt gcaggcagtc agcaggtatc aacgacactg gtacattttg
     1981 ccgctgatgc tggcgttatt caggaattta ccccgctgat gctggtggac aatggcgagt
     2041 ttaagccgtg ggatggtcag gaatctggca aggctgttta tctgacttcg tacctcgtgg
     2101 acacgtcaaa gcagaaatca gcacagtgtt acaagacggg gatattcaat attgccgccg
     2161 ttaactggcc tgagagcgtc gacaccgatg cgaaaaaatg cgccgccttt gcgggttctg
     2221 gcgtatccgt tcagccgctg gcccgataag cagggggaac gatggcaacg aatgaaagca
     2281 tcatggcgct accgctggcg agtaaattta aagccgaagc gcgggcaatg gctgacagag
     2341 gtttatcaac ctacgaggcc gtatatcaac tcaacaaact ggaagagcag gacaagccgc
     2401 gcgctgatgc gattatggcg cttcatgagc ataaagacta tcagccgctg ttgcgtgcaa
     2461 tggcaaacgt gccttgtatc gatgttgata cggctaaaaa catcctgaac atgaccatag
     2521 agcaggaacg cccgaaggtt gcaccagagc ttaccgcagc ctttgaaaac tttatggaca
     2581 tgcacagccc acaaaccgta tcagctggca tggcatacga tggcagaaac ccaggcgatg
     2641 acggcgacat cgatcgcata ctgaaaacca tctgagacaa ggccggagaa atccggcttt
     2701 tttgcgggtc cttcctggaa ttatggcccg ttacggggcg gcgacctcgc gggttttcgc
     2761 tatttatgac gtttttccgt gaaggtgaca ccaccaccac ttgattaata tttaaccatg
     2821 cagttaaggt aacattatga ttgataaagc ttgttttgta agtcagcagg aaatagctga
     2881 acatttcaag gttaacagaa ccactattcg cgcatggacc aaacagggga tgccgtatct
     2941 taatgcggat cgcggaaagt ctggcggtta tcacatcggg catacattgc tttggtcttc
     3001 aggtaaaagc cgtcttgagg ccatcagata tcacgtagaa accagtgcgc tggaaaaaat
     3061 tatgtttgcc aggctgcttt catctgagcg tgacgagtac tccagtgaag aaacagaaca
     3121 tcgatttgat gaaggtttgc agatttacgg ctattcaccg gaagatgtga gcaaggcacg
     3181 aaataaaatg gctagctttc tggctgggtg gcgtcatgcc gtaagcgttc gccgtgccag
     3241 catggagcaa tcagccgata cagaacagta aagccgataa cttgttaatc actcgcagca
     3301 atgcatgtga tttgtgcaca ctttcggtta actggttcga tgtccggttt tagtgtctgt
     3361 tttttgcgca tgtccggttc atggaaagca tgtttttata tttttcatat ggttaacttg
     3421 tagagaaacc ggacatggat cccgaaaaat tttcataaat agtgaaaacg cgcgaggtcg
     3481 ccgccccgta acgggtccat aattccagga aggacccgac gacaccagac aatcagaacg
     3541 ataggtgcac aatgacagaa gccgaaatac tgggattaat ccgccgtgct ggtggaatca
     3601 gccagcagac tgacgaacag gccacgcagc cggacagcgt gacagccgaa aattatgcgc
     3661 gtgttgttgc tgaggtgatg cgccgtgatg gtatccagct taatgatgtg gatatgcgca
     3721 acatacggat ccgcgttctt gaaatgctgg cctacaatcg ccgcgtggca ctgtatcggg
     3781 aaacggagaa aattacatac cactggaaga agccggagcg gttgcggcgg taactggttg
     3841 atattttcga aattcgccag cggcgaacat cggggaaacc agcctaaccc gttgatattt
     3901 tcgggaacct caaattgagg aggtcggaaa cataacccgt catattctgg atgctgataa
     3961 ttaccacgca gcccattaag gcctaacccg ttgattcttt cggaaacagc cattggcgta
     4021 atccggcgcg gtaacttgct gaacttaaag caaagcgcaa aattgcgctg gctggtgggc
     4081 atgatgccga tatggtgatc cccataacgg aaatatccac cagcacggca acggcagaat
     4141 agccacaaca cagaaaaacc acgaatatgg ggtttttgtt atggcatggg catgatgacc
     4201 gcccctgctc tgaatggtgg aaatcaccac gcagatcatc caccagcttc gtttttataa
     4261 attaaacatc cgggaaaatc cgaccctgaa ttgtgcattt tttcagcaaa tcattttagt
     4321 tatacgtttg gttatacggt ttaaaagttg aatcatatac attattttaa atcatgaagt
     4381 tacacgctca attaaactac tctcgtgaaa aaccaacctt tacggttggt ttttttatat
     4441 ctgcaattaa tttgataaac agaccgtgac acatcacagc ctgtttattt tctgttatca
     4501 gaacgtccag accactcccg cctgagtatt ccacggcgat tctacccctg cgccattgcc
     4561 atagctgact gacaaatgac cgcttaacgt cggggtaaac gatgacctta tcccagcctg
     4621 ataaacgcca ggggttccag ccacatcatt attaaatttg ccatcgtcat taactttcac
     4681 atggttagaa tcggcgtatt cctgacgtac ggcggctttc agccagggtt ccagcgtcgt
     4741 accattttgc aggtccatgt gatagcttac cgccgttccc gcttcagcgc gtaatatccg
     4801 ggtatttccc acatcagcgc gcatgccgtt tgataacgtg tagtcctgac catctgtggt
     4861 aaagccggta aaggccagat agggtctaac actccacaat ccgtcaaccc aacggaaccc
     4921 gctctcaaca tgagcacccg cgccgttact attgtaatcg ccaaacgctg ttgccccatt
     4981 actcatcttg ccatggatgg tgttggcaaa acggtcaact ttcaccaccc catcaacata
     5041 ggcaccgttc tgatgctccc aaccggcata agcccccagg gtatagctat cgatattacc
     5101 ttttccgccg cgatcaaaac caatatcaga atgagagtaa ccaaagaaca agccgcgaat
     5161 tgtactgctt tcttcacggg agaaacggct atcgataccg agcgtcaggc ccgtcaatgt
     5221 ttgctcaaaa ccagctcccg catcagtggt cacgttgttg cgggtgttaa ttgccgaact
     5281 ccacatcgcc gtatcgtaac taacgctttt tacgctacca agacgctcac gcacggtgtc
     5341 cagttctgca tcaaatacca gcggttgtgc ggccgccata ttcagcacat cagtggttga
     5401 aggggtaatt tgcgcgcgat tctctgccaa actccagcta tggttgccat tatccagcaa
     5461 ggtatattca tacgtaccga tatcaacaac gcctccggca ttgcccaacg taaatgcagc
     5521 atcaccgccg cccgttgtta ccagtgtaag gctatctcct gctgccgggc tggcaccggt
     5581 gtccgtcacg aatattttga aatcacctgt tgcctgaccg gtgacgttga gctgatcgct
     5641 ctgatgatta gccatatcgg tacgcatata aaaattgccg tttcccgaca gggtattggt
     5701 ggtcaacgta atgaaatttt ccgcacttgc cgtcactgat gaacgagtca ctggctcatc
     5761 catcataatc acatttgcgt cagacatcgc aaggttggct aagttgagga gggtgttttg
     5821 gttcgtcgta gtgatatcgg caatgatggc ccatacgcta ttttcgagcg aaacgtccgc
     5881 tttgctggta tccacggcac catgcgttct aaaagaagcg ccctcagaaa ccactacctt
     5941 acccaccaac atagcgggtg cataatttat ttgcggcttc atgacctcaa gaatgccatc
     6001 attccctctg actgacacgt taaccattgt gccagcccag acattagcgc ggccattgtt
     6061 gataaccata ttttcagaga tggctttaga ggaatacgtg atacttccat tctggtctga
     6121 tcgaccgaga tcatataccg catttgcctg cactttcgta ccagtatcct ttcccagcga
     6181 ttgcatagtg gcatgctcat caaggatagt atcaatggcc tgcgtgtcct ccataacaat
     6241 gagcccggaa ccatcatcca gttcataatt ttttgacaca ccatctttta tactaaattg
     6301 gcctttactg tttgtaccac tcacttccag cgcattcgtt gagacaatta attttccgcc
     6361 tgctttttta tcaacgccag ttgcagtccc gccatccata acctccagta agccgccact
     6421 atctacagta gtattataag cgaagtcatt ttcttcaact cgcaaacttc cgccgttttc
     6481 caacagcata ttgttagcaa taccattctt gatttcgaac tgaccgagac ggtttgttcc
     6541 gaatgcctcc atgacccgcg tgcttgcttt aatagcaccg cctgctttct gatctaccgc
     6601 aatcgctaat cccccctctt taacctccag taatcctccc ttttcgatag cggcaaggac
     6661 ggtaatccca tctttttcaa cgattaagcg cccgccttta atcgtgggat cattcgctgc
     6721 gccataaacc tgtagcacac cattattgag ggtagtctgg tttgccgcgc cacccatttg
     6781 aacaatttga taaccgtcat ttaatactga gtattctgcg gtaccattca tttcaatgta
     6841 ctgataccct ccgt
//
LOCUS       DADLTD010000203         6773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLTD010000203 DADLTD010000000
VERSION     DADLTD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.205"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..393)
                     /locus_tag="JN701_003478"
     CDS             complement(<1..393)
                     /locus_tag="JN701_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7365799.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAG"
     gene            complement(415..741)
                     /locus_tag="JN701_003479"
     CDS             complement(415..741)
                     /locus_tag="JN701_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY7365800.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(960..1925)
                     /gene="glk"
                     /locus_tag="JN701_003480"
     CDS             complement(960..1925)
                     /gene="glk"
                     /locus_tag="JN701_003480"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY7365801.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            2129..3385
                     /locus_tag="JN701_003481"
     CDS             2129..3385
                     /locus_tag="JN701_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY7365802.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            3500..3826
                     /locus_tag="JN701_003482"
     CDS             3500..3826
                     /locus_tag="JN701_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7365803.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(3967..5205)
                     /gene="mntH"
                     /locus_tag="JN701_003483"
     CDS             complement(3967..5205)
                     /gene="mntH"
                     /locus_tag="JN701_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY7365804.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            5541..6743
                     /gene="nupC"
                     /locus_tag="JN701_003484"
     CDS             5541..6743
                     /gene="nupC"
                     /locus_tag="JN701_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY7365805.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNTLFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         1685 a         1779 c         1635 g         1674 t
ORIGIN      
        1 accagccggg aacgccagtt tgccaccgat agagttcgcc acaaaagcag caaacatcgg
       61 aatggcaaaa ccaaacaaca cgccgccgaa ggactgcgac agccaggcaa atttcagtaa
      121 ggagaggtcg aaaccggaga atttcccgct attaagagca tccatgatgc cgatctcagc
      181 aggaatttta agccagctat aagcaatcag ctggctgaaa gcgaggatca caccgcccat
      241 aattaaggtc ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga gttcacccca
      301 gaagctgctt ttagaggcct gcggattctt aactgccgct gccgcaccgg atgcgccagg
      361 cacaacggtt gcactgcgtt ttttaatggc cataacaatt atccctgtcg aatattactg
      421 ttgttcagag gcaatcatct cttcgatttc tttgatgatg cccgcagcgt ttttaattgc
      481 gtcctgcaaa gtgatttcat aaacgtcgcg tgattcgaaa cgctcattat cttccggggt
      541 aactgccacg gagtggatga tgatggttgc ttcggcgata tcctgcgccg tcaggcgatt
      601 ctggataccg tccgcgccct gggtttcaat tttcacttca taaccggctt ctaccgccgc
      661 ttcttccaac gcctgagcgg ccataaaggt gtgagccagg cccattgggc aggcacataa
      721 ggcaatcagt ttcttactca tcgttaattc ctcattcagt gatagttgac aagtgaaatt
      781 atgaacgtgc aaaacaaatc gccgttacca gacaaataat gcatttactg gagaattaat
      841 cccgctgtac ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag aaaaattgaa
      901 ttaactgtat gatttaaacg attatcggga gagttacctc ccgatataac aggaaggatt
      961 tacagaatgt gccctaaggt ctggcgtaaa tgtgcaccgg aaccgagaag acccggattg
     1021 tcatggacga tgagatacac cggaatatca tggacatatt ctttaaagcg ccctttatct
     1081 tcaaatgcag cacggaaacc agaggctttg aagaactcaa ggaagcgcgg cacgataccg
     1141 cccgcaataa acacgccgcc aaatgtcccg agattgagcg ccagattgcc gccaaaacgg
     1201 cccataatga cgcaaaacag cgacaacgcg cggcggcaat cggtacagct gtcagccagc
     1261 gcgcgttcgg taatatcttt tggcttgaga ttttctggca ggcggttgtc agctttcaca
     1321 attgcgcgat acaaattcac cagcccaggg ccagaaagca cgcgctccgc cgaaacatga
     1381 ccaatttccg cacgcaatat ttcgaggata atggcctctt cttcactatt cggcgcaaaa
     1441 tcaacgtgac cgccttcgcc cggcaagctt acccaacgct tatcgacatg gaccagatgc
     1501 gcaaccccaa gccctgttcc ggcaccgtaa accgcaatag gtttaccttc taccggttct
     1561 gcgccaccaa actgaatcag atgctctttt ttcagcatcg ggatcgccat cgatacagcg
     1621 gtaaaatcgt taataatttc cagatggcta aaaccgagat tctttttcat ttcggcaatt
     1681 gagaacgccc aggtatggtt ggtcattgcc acccagtcac cggtaattgg gcaagcgatg
     1741 gcaatacagc cgtctttcac ctcgacctta tgttcttcaa gataaacgcg aatgaccgct
     1801 tcgaggctgg ggtaatcaag ccctgaatag gtcttagcct gcgagatttc accactggca
     1861 atatcacaca gagcaagacg tgcgttggtg ccgcccacat caccgactaa tgcatacttt
     1921 atcattcttc aactgctccg ctaaagtcaa aataattctt tctcacactg taaatacctg
     1981 ggggcataac aacaacgccg gaaaggcagg ctccctgtaa atatcgatct gggtcacaca
     2041 tttactttat cgtttcagca ccaattgcag cgatgccttt ttgcaagctg ggcaaactaa
     2101 gtatctgacc ccgcataagg aatagaacat gctccatccg cgagccagaa ccatgttgtt
     2161 attatcgctc cccgccgtgg caattgggat tgcgtccagt cttattctga ttgtggtgat
     2221 gaaaatcgcc tcggtattac agaatttgct ctggcaacga ctgccgggaa ctctggggat
     2281 agcccaggat tcacctctct ggatcatcgg tgtattaacg ctaacgggta ttgcggtggg
     2341 gttggttatc cgtttcagcc agggtcatgc cggaccagac cccgcctgtg aaccgctgat
     2401 cggcgcacca gttccgccct ctgcgctacc aggacttatc gtagcattaa ttctcggtct
     2461 tgctggcggc gtcagcctgg ggccggaaca tccgatcatg accatcaata tcgcccttgc
     2521 cgtcgccatt ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga ctattttagc
     2581 ctctgccgga accatcggcg cgctgtttgg cacacctgtt gcagcggcgt tgatattttc
     2641 gcaaacctta aatggcagta gtgaagttcc gctatgggat cgtctctttg cgccgttaat
     2701 ggcggcagca gctggtgcac taactaccgg attatttttc catccccatt tttcactgcc
     2761 cattgcgcat tacggacaga tggaaatgac cgatattctc agcggtgcaa ttgtcgcggc
     2821 gattgccatc gcagcaggga tggtcgccgt atggtgctta ccacggttgc acgcgatgat
     2881 gaatcaaatg aaaaatccgg tgctcgtgct gggtattggc ggatttattc tcggtattct
     2941 gggggttatt ggtggaccag tttcgctgtt taaagggctg gatgagatgc agcagatggt
     3001 ggcaaatcag gctttcagca ccagcgatta ctttttgctg gcggtaatta aacttgccgc
     3061 cctggtcgtt gctgccgcca gtggctttcg cggtgggcga atcttcccgg cagtgtttgt
     3121 cggcgtggca ttagggttga tgctgcatga gcacgtaccc gccgtaccag cggcaataac
     3181 cgtttcttgc gctattctcg gcatcgtgct ggtggtaaca cgcgatggct ggttaagtct
     3241 ttttatggcg gcggtcgttg tacccaatac cacattgcta ccgctgctct gtatcgtcat
     3301 gcttccggca tggctgttat tagcaggtaa gccgatgatg atggtcaatc gtccgaagca
     3361 acagccaccc cacgataacg tttagcaaaa aatgctctcc tttatgattt aagagttatg
     3421 gcgatgatac ggggctttac ctccccgtaa tattgcgtta acaggccgct gacggcttcg
     3481 atcgtgaagg agaataacaa tgttcaggtc actgtttctg gcggccgccc tgatggcatt
     3541 taccccgctt gcagcaaacg caggtgaaat caccctactg ccatcaatca aattacaaat
     3601 tggcgatcgc gatcattacg gtaattactg ggacggtggt cactggcgcg accgtgacta
     3661 ctggcatcgc aattatgagt ggcgcaaaaa ccgttggtgg cgtcatgata atggctatca
     3721 tcgtggctgg gataagcgta aagcgtatga gcgtggctat cgtgaaggct ggcgcgatcg
     3781 tgacgatcat cgcggaaaag gccgcggaca tggacaccgc cattaaacac attcgccaat
     3841 ggagcacaat gcctgatgcg acgctagcgc gtcttatcag gcctacaaac cggatcttta
     3901 acgcgtaggg cggataaggc gtttacgccg catccgccaa tagtgccaga tgcgacgctc
     3961 attcaactac aatcccagcg ccgtccccac caacaaccag atattcagcg ccacgaccag
     4021 cactacaatc acccagcctg tctgttttac gcgtttgctg ttcaccagat cgcccatcaa
     4081 cttgctgtca ctggtgaaaa tcagcagtgg aaccagcgcc agggcgatac caaaacttaa
     4141 cagcacctga ctcataacca gaatccgtgt cggatctaat cccatcagaa tgacaataaa
     4201 tgacggcagc atggtgactg tacgacgcac ccacagcggg atatggaagc gaatgaaccc
     4261 ctgcatcacc acctgccctg ccagtgtccc cactaccgtt gaggacagtc cggcagcaac
     4321 cagacttaac ccaaagaccg ttgccgcagc atggcttaac agcggttgca gcgtcagata
     4381 agcctcatca agatcggcaa caccagtatg accagaaaag tggaacgccg ccgcagctgt
     4441 agccatcatc gccagattga caaaaccggc aatagtcatg gcgatagcca catcccattt
     4501 ggtggcggaa taacgttgtt gacgcgaacc gccatgtaaa tgctgagtga gcgaggagtg
     4561 caaataaatc acatgcggca taatcgtcgc ccctaacacg cctgctgcca ggaagaccgc
     4621 ttccgaagta ggtaaactcg ggatcaccat tcctttaccc agctgcgcca ggttaggctg
     4681 ggagaaaatc aactcgacaa tgtaagccgc ggcaacaaac aacagtaacc cgccaatcac
     4741 tttctccagc ggtttttgcc cgcgacgttg cagcattaaa atcaggaaag tcgcgatccc
     4801 cgtcagcacc gcgccctgca acaacgaaac accaagaatg agtttaaaac cgatcgccgc
     4861 accaataaat tccgccaggt cggttgccat cgcaataatt tctgcctgaa cccaatagaa
     4921 ccacactacg ggacgcggat agtgatcgcg aatctgctcc gccagatttt taccggtggc
     4981 aatccctagt ttggcagaga ggatctgaat cagcatcgcc atcaggttgg cccaaacgac
     5041 aacccacagt agctgatagc cgaagctggc acccgcctga atattggtcg caaagttacc
     5101 gggatcgata taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga gcctcatctt
     5161 gcgcgccgcc cgtccgctgc tactctcaac gcgatagttc gtcatcttgt gcctctaaaa
     5221 catagccttt gctatgtttc atgctatgat aaacgagaat gattatcaaa ttcatttaaa
     5281 tggattgtgg tgatttctgt gatagaccag gattatgact acgaaaagat tgatgacttg
     5341 aatgttatgg ttatgtttaa tgttagcaca tttacataac tttcagcttc catacacaac
     5401 atagcagaaa tgtatgacag atcactattt ttgaagcctg tcacaggacg tcattatagt
     5461 gtgtgtcaga tctcgttttc cttaaccatg ttacatagaa tgtgcacgga aatttaacct
     5521 gcctcatatt tggagcaaat atggaccgcg tccttcattt tgtactggca cttgccgttg
     5581 ttgcgattct cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc cgttatgtta
     5641 ttcaactgct tgttatcgaa gtgttactgg cgtggttctt cctgaactcc gacgttggtt
     5701 taggcttcgt gaaaggcttc tccgaaatgt tcgaaaaact gctcggattt gccaacgaag
     5761 ggactaactt cgtctttggt agcatgaatg atcaaggcct ggcattcttc ttcctgaaag
     5821 tgctgtgccc aatcgtcttt atctctgcac tgatcggtat tctccagcac attcgcgtgt
     5881 tgccggtgat catccgcgca attggtttcc tgctctccaa agtcaacggc atgggcaaac
     5941 tggaatcctt taacgccgtc agctccctga ttctgggtca gtctgaaaac tttattgcct
     6001 ataaagatat cctcggcaaa atctcccgta atcgtatgta caccatggct gccacggcaa
     6061 tgtccaccgt gtcgatgtcc atcgttggtg catacatgac catgctggaa ccgaaatacg
     6121 tcgttgctgc gctggtattg aacatgttca gcacctttat cgtgctgtcg ctgatcaacc
     6181 cttaccgtgt tgatgccagt gaagaaaaca ttcagatgtc caacctgcac gaaggtcaga
     6241 gcttcttcga aatgctgggt gaatacattc tggcaggttt caaagttgcc attatcgttg
     6301 ccgcgatgct gatcggcttt atcgccctga tcgctgcgct gaacacactg tttgccaccg
     6361 tgactggctg gtttggctac agcatctcct tccagggcat tctgggctac atcttctatc
     6421 cgattgcatg ggtgatgggt gttccttcca gtgaagcact gcaagtgggc agtatcatgg
     6481 cgaccaaact ggtttccaac gagttcgttg cgatgatgga tctgcagaaa attgcttcca
     6541 cgctctctcc gcgtgctgaa ggcatcatct ctgtgttcct ggtttccttc gctaacttct
     6601 cttcaatcgg gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa ggtaacgtgg
     6661 tttctcgctt cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg ctgtctgcgt
     6721 caatcgcagc actggtgctg taagaccata cataaaaaag ccggggataa ttc
//
LOCUS       DADLTD010000204         6759 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLTD010000204 DADLTD010000000
VERSION     DADLTD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.206"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..297
                     /locus_tag="JN701_003485"
     CDS             <1..297
                     /locus_tag="JN701_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7365806.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(448..3771)
                     /gene="mscM"
                     /locus_tag="JN701_003486"
     CDS             complement(448..3771)
                     /gene="mscM"
                     /locus_tag="JN701_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7365807.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQEL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3793..4761)
                     /gene="psd"
                     /locus_tag="JN701_003487"
     CDS             complement(3793..4761)
                     /gene="psd"
                     /locus_tag="JN701_003487"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7365808.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(4858..5910)
                     /gene="rsgA"
                     /locus_tag="JN701_003488"
     CDS             complement(4858..5910)
                     /gene="rsgA"
                     /locus_tag="JN701_003488"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7365809.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6005..6550
                     /gene="orn"
                     /locus_tag="JN701_003489"
     CDS             6005..6550
                     /gene="orn"
                     /locus_tag="JN701_003489"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7365810.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1561 a         1817 c         1814 g         1567 t
ORIGIN      
        1 cttccttacc tgttcctcgc gctggctttc ccattcttta aagcacgtca ggatctcgac
       61 agaccatttg tgattttcaa aacgcgtatg tcggcaatga ttgcgacggt ggttgtcgta
      121 ctggtggtga catttgcgaa cgtcttcacc attattccgc ctgtggttga agccggagac
      181 tgggacagca cattgtggat gattggcggc cctgtcttct tctcgctgtt agcgatggcg
      241 atttaccaga actattgcag tcgcatggcg aataaacctg agttagctct cgactgatca
      301 tatgaaatcc tgcaactcat cgtctctccg gcgatgagtt gtcaggaagt aatgcgtatg
      361 gatgatactc tctagggccg gtgataactg aagatgatgt taagctgcta aatgagccac
      421 gttgacatca tttggcagct tcatgcatta caaacttccc gcctgacgac ctttgcccgc
      481 agacgtcagc gttctccccg tttgtttacc gttgaggctt tccagacgca tctggaaggg
      541 cgggaatggc atatcgatac cgtgggcatg gaagccagcc agaatcagct ggtggatctc
      601 atggcgtagc ggcatacggt gacccatctc agcggcataa atacgcagct cgaaaatctg
      661 aatcccttgt tgcagatcca ccaggaagac ttccggtgcc gggttgtcga tcaccagcga
      721 gcagcgacgc gctgcggtga gcaggatttc cgtcacttct tcactgttgg catcagcagg
      781 ggccggaatc gtcaacacta cgcgcgtaac cgagtcagag agcgaccagt tgataaactg
      841 ctcggtaata aacgccttgt tcggcacgat tatctcttta cggtcccagt cgctgatggt
      901 ggtggcgcgg gtgttaattt tcgtcacgct accggtgaga tcgcgaattg tcaccgtatc
      961 gccaatgcga atcggttttt cgaacaggat aatcagacca gagataaagt tggcgaaaat
     1021 ttcctgcaaa ccaaaaccga gaccaacacc gagcgcggcg accagccact gcaatttcga
     1081 ccattcaata ccaatcatcg agaagccgac cagcccgcca atcagcatca gcagatattt
     1141 ggtgatggtg gtgatggcgt agcctgtacc cggcgttaaa tccaggtgct gcaaaatcgc
     1201 cagttccagc agcgcgggca ggttgcgcac cagctgcgtg gtgatgataa acaccagaat
     1261 ggcaatcagc accgcaccga gggtaattgg ctccagactt tctacgcctt gtaccgtgga
     1321 ggtgacatcc cacagcgaaa tattttcgag gaagccgaaa gcggaatgga tttctgacca
     1381 cagcacaatg acagaaagca gggcgatcaa catcaaaatt gagcgcacca gccgcaagga
     1441 ttgcgcactg atggcatcga gatcgacttc gctttcatcg acttcaattg ctccttccgg
     1501 gctactgtga tgatgcgctt cctcttcgcc acgcgcacgt tgcgctaaca tctctgcccg
     1561 gcgatgcttc gcccggtcaa acgccagcct gcggcgctgg atcagcatcc agcggcggat
     1621 aacgtgataa accaccagta gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa
     1681 cagcgcctgt gccgttgcca gataacccac agccgacgcc agaatggcaa ccaacggcgc
     1741 gccaatcatc atgttccaca gcatatggtt ggtaatgttg tcgccgctgc cctctttgtt
     1801 gagatacagt gggatcccgg cctttttcag gctgagggtg accaccgcca gcgcaccgca
     1861 aatgaggata aagcaaagcc gtcccagcga accggagaat tcacggtcgt cgaggttatc
     1921 gaacatcatc agcgccataa tcagcggcac aataagcccg atgctcatca ggtagtagcg
     1981 catcccacgg gaaacacgtt cgcgcggcca gccaaaatga gcgataaaca agccgttcgg
     2041 gcgggcaaag gtggcgcaaa tcatcactac ccacagcagc ggcacggtag ccgttacacc
     2101 atcgccaatc gcgaccgcca gcggataagg ccacgcctcg cgcaagccgt aacccagcgt
     2161 catccacagc accggtaacg gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt
     2221 cagccagaag tgatcctgag tcactttgcc aactttcgcc gccgaacgtt caagaaaacg
     2281 ggtgaaatag cggcgcgagt aaatactgca accgaccaga atcaacgcgc caaacagcgg
     2341 caaaattgtc tctttgctgg tcagcatcat cacactggct ttgcccaact gactgaaggt
     2401 gtccagcgaa atgagacgac gcagctcctg ggcgatttcc agcggccagg cgatggtcat
     2461 cgggcgcacg tcagaggtcc agaacagata gcggtgcgtt gcttcgttca cctctttcag
     2521 cgcatcctcc agttgcccgt tggagacttt cagcttggtc agttccaata gtagcgtgtc
     2581 gccaccctgc aacaatgagt tcagcaactc acgctgagtg cgcagttgtg cttccagaat
     2641 acggttttgc tcggcggtca gcggctgacc gtcggcctga tggatttgcc gtagcagcgg
     2701 ctgtttatta agcagatcct cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt
     2761 atcaagctgt tgtggttttg gcatttctgg cagccgtgcc acctgcgccc gcagcgcttc
     2821 gccgagcaga ttggacgatc ccagccattg cgactgttca cgcagcgtat tcaacgcctg
     2881 ccggacctgt aacgtctggc tggcagcctg acgctgttgc gaggcaacga gatccatccg
     2941 ctgcgcctgt tgattcaaag ccgccgatag ttcgcggtta attttgaatt gcgcgacgat
     3001 atctttcggc aaatcggcgc tgttttctgc cagcaattcg gtactttcca gcgcccgctc
     3061 cgcctcaagc tgacgttggc tgtttaattg attacgcaag gcctgcaaat acgcatccag
     3121 ttgctggctc tctttttccg ccagctctga gcgtaagcgc gctaattcct ggcggttatt
     3181 ggcagacagc tgcgccagct ccagttcatc aacgagcgcc ttaagacgtg cagagtcaga
     3241 ctgcaacgcg aaattttgtg cctgattgag cggagtattg ccggtaagcg ttcccaggcg
     3301 gcgctcgatc tcatttaact gacggcgggc gtcggtttgc tgttgcggca gttgattcag
     3361 cgaatcggca atctcgcggg tgcgctcctg ctcttgctgg gcctgacggc ttttatccag
     3421 caactggctg ctgacctgga gaatttcctg attcagcgcg tcggtagaca ttcccggcga
     3481 cacgctgcgc ggctcgtcac gcatgttgtt taattgtgcg cgcagagtag cggagagttt
     3541 cggataatta tcgataactt gctgatattg tttgatgcgc tcaagggaac cttttcgttc
     3601 ctcaagcgca tttaaggcag actggagcgc ctctacgact tccggctgtg cgggtttcgc
     3661 cgcttttgcc tgctccagtt cctgagtgat ttgtttgcta tcgggggccg tcgcggcgta
     3721 cgccccccaa ctgaggcacc aggccatcag aaaagtgata atcaggcgca cgtcagcgtt
     3781 tcctttgatg gattagacct ggtctttttt atcgtcaacc aatgggctgg cgtcgtgttc
     3841 tgcttcgatc tcttcagcag gaagtggggc gggttcagcg tctggcgtaa caaaggtttc
     3901 ggtagatact gccagcggct ggccaatttt cgtgacagac aggctttcca gttgctcaac
     3961 cagattcact ttacccggtg caaacaggtt gataacggtg gaaccgagtt taaagcgacc
     4021 catttcctgg cctttcagca gtgccacaga accgtcgttt tccccggcag gccaggtcca
     4081 gcgcttgatg ataccttcgc gcggcggcgt aatggtgccc gcccagaccg tctcaatgct
     4141 gccaacaatc gtcgctccga ccagaatctg cgccattggg ccaaattcgg tatcgaaaag
     4201 gcaaatcacg cgttcgttac gggcaaacag attcggcacg ttctgagcgg tgagatggtt
     4261 aacggagaag agatcgcccg gcacgtagat catctcacgc agaataccgt tgcacggcat
     4321 gtgtacgcga tggtagtcac gcggggagag gtaagtggtc acaaacgtac cgttgcggaa
     4381 caggtccgcc atcagatagt tgcctgccag cagggcttcg aggctgtagt tgtggccttt
     4441 ggcttgcagg attttatctt cttcgatttt acccaactgg ctgataacgc catcggcagg
     4501 catgaccagt acattcggat cggtatcgat tgggcgtact tcgtcacgca gcggacggac
     4561 aaagaattcg ttaaaggtgc ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc
     4621 gaccttgtag tatttaacga acagatcgat aaccagtttt gtcagccatc ctgcccgctt
     4681 gcttgcgccc caacccgcca ggcgagtaag ccatagtttc ggcagaatgt actgtagcga
     4741 aagtttaaat gaatttaaca aggtagcctc caggccattg ttttgtcgtt cctgatccgg
     4801 ccaacatgcc ggatcctgaa aaaaagggga cgattctaac gacggttagc ttaattgtca
     4861 gtcatccgta tcagaaaagt ttttacgcgt ttttacctgc gccatgcttt ccagaatacg
     4921 gtgatagttt tcgaaacggg tttccgcgat tttcccttcc tcaaccgctt cccggatagc
     4981 gcagcccgga tcggtatcgt gtttgcaatc gcgatattta cacagaccta aatagtcatg
     5041 gaattcgaca aagccctgag tgatttgttc cggctccagg tgccagaggc cgaactcacg
     5101 cacaccaggg gaatcaatca catcaccgcc gtgcgggaag tgatacagcc gagcggcggt
     5161 cgtggtgtgc tgaccgagcc ccgagttgtc agagacatcg ttggtcagga tctctttttg
     5221 cagccccagc aacgcattca gcaggctgga tttgccgacg ccagactgcc cggcaaaaat
     5281 gctgatgcgc ccggtcaacg cctcttccag cggttttagc ccatcctgag tatggctgga
     5341 aaccatcaat acgcgataac cgatattgcg gtagatatcc atctgctcgt tgacgaacgc
     5401 catgccttcg tcgtccagca ggtctatctt gttgagcaca ataatcggct caatctgcaa
     5461 ggtttcgcag gccaccaggt aacggtcgat aatattgagt gacagctccg gcaaaatggc
     5521 ggagacaatg acaatctggt cgatgttggc ggcaataggt tttacgccgt cgtagaaatc
     5581 cggacgcgtc aacaccgagg tacgctcatg caccgcttcc acgatccctt tgacatttac
     5641 gccttccgcc gccggtttac ccggacgcca gactacacgg tcgccggtca ccagcgaacg
     5701 gatggtacgg cgaatattgc agcggtgaac gtcgccatcg gcggattcca catcagcgtg
     5761 cataccaaag cggctgatga cgataccttc atcaggctcg ccaaacagat tgtcgtcgta
     5821 gtcgggcttc tccttagacg ttttaagacg acgctggtga ttggcgttca cgcggcgctg
     5881 ctggcctttg gagagtttat ttttactcaa tcgtacagac tcctggtcgc ccctgatggg
     5941 caaaacatct atgatacacg caattgtgga tcaatatagt cactgtgaat gggtggaaaa
     6001 tagcatgagt gccaatgaaa acaacctgat ttggatcgat cttgagatga ctggtctgga
     6061 tcccgagcgc gatcgcatta ttgagattgc cacgctggtg accgatgcca acctgaatat
     6121 tctggcagaa gggccgacca ttgcagtaca tcagtccgat gaacagctgg cgctgatgga
     6181 tgactggaac gtgcgcaccc ataccgccag cgggctggta gagcgcgtga aagcgagcac
     6241 gatgggcgat cgggaagctg aactggcaac gctcgaattc ttaaaacagt gggtgcctgc
     6301 gggaaaatcg ccgatttgcg gtaacagcat cggacaggac cgtcgtttcc tgtttaaata
     6361 catgccggag ctggaagcct acttccacta ccgttatctc gatgtcagca ccctgaaaga
     6421 gctggcgcgc cgctggaagc cggaaattct ggatagtttt accaagcagg ggacgcatca
     6481 ggcgatggat gatatccgtg aatcggtggc ggagctggct tactaccgcg agcattttat
     6541 caagctgtaa aatttttcct ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt
     6601 ttgacgctaa attgaacgtc ttgctgataa tttcagcact tgagataaaa acgcaaaaaa
     6661 aacttttttg gggggttgca gagggaaaga tttctcgtat aatgcgcctc ccgtaacgac
     6721 gcagaaatgc gaaaattacg aaagcaaaat taagtagta
//
LOCUS       DADLTD010000205         6625 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLTD010000205 DADLTD010000000
VERSION     DADLTD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.207"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..357
                     /locus_tag="JN701_003490"
     CDS             <1..357
                     /locus_tag="JN701_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365811.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
     gene            379..1557
                     /locus_tag="JN701_003491"
                     /pseudo
     CDS             379..1557
                     /locus_tag="JN701_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(1944..2320)
                     /gene="rnpB"
                     /locus_tag="JN701_003492"
     ncRNA           complement(1944..2320)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JN701_003492"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2353..3498)
                     /gene="garK"
                     /locus_tag="JN701_003493"
     CDS             complement(2353..3498)
                     /gene="garK"
                     /locus_tag="JN701_003493"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY7365812.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3595..4485)
                     /gene="garR"
                     /locus_tag="JN701_003494"
     CDS             complement(3595..4485)
                     /gene="garR"
                     /locus_tag="JN701_003494"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7365813.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4515..5285)
                     /gene="garL"
                     /locus_tag="JN701_003495"
     CDS             complement(4515..5285)
                     /gene="garL"
                     /locus_tag="JN701_003495"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY7365814.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(5301..6599)
                     /locus_tag="JN701_003496"
     CDS             complement(5301..6599)
                     /locus_tag="JN701_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365815.1"
                     /translation="MHTRYLILLIIFIVTAVNYADCATLSIAGTEVAKELQLSTVSMG
                     YIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGIS
                     MFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLT
                     FAWGWEHVFTVMGVIGFVLTALWIKLIYNPTDHPRMSAEELKFISENGAVVDMDHKKP
                     GSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKV
                     GLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNN
                     TTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGY
                     LVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1811 a         1725 c         1444 g         1645 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
      361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
      421 ttagtccgtc gaaaaatgaa tgaccttcct gaaatcccca tagaaaataa tctcgaagca
      481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
      541 gcgttacaac caggtggaaa atgtattatc actggtgatt ttcaaaaaga attagcgtac
      601 ttacaaaatg taattcttta taatgactcg tttcttcgtc tggatttttt aggttataac
      661 gcccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
      721 aaccaggaaa tatctacagg caatatcaat gttaattgtc cattaaaaga tatttacaat
      781 gaaatcagga ggttgaacgc aacctttagt tgtgggactg gagatatagt tgatctatcc
      841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
      901 aatactattc taaatccttt taaattggat tcaacaaatt ttactaatgc caacatgttt
      961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
     1021 ataacgcctg ttttaataag tattagcgat atgtattctg aagaaaaaat caagtttgtc
     1081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
     1141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
     1201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
     1261 gagatgaaag aattctcatc aaaaaaaaac atatgattac atcgatataa tcattgaaga
     1321 ttatgaaaat atcaaagaaa atgcttatct ggtcgtcccc caaattaatt atactatgga
     1381 tttaaacata gaagactcca gctcagaaga gttaataccc tcgagaaaga cgaaaattct
     1441 tcggacaatg gctttgaagt cgaagaatat aacgcatatg aagcatataa ctcagagaat
     1501 ctatatttta ccagagagga atatacgtat gattacgacc tttcaaatgc aatatagact
     1561 taataacttc ttacaaaatc tataattaat accgtagata ttcattcatc ttaccctgtg
     1621 caccaaaagc aaaaactcac tgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct
     1681 gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt
     1741 gtaggcaggt aaggcgttca cgccgcatcc ggcatgaata aagcgcactc aacaatctga
     1801 aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
     1861 ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
     1921 cgaagcgggt ttttacgtaa atcaggtgaa actgaccgat aagccgggtt ctgtcgtgga
     1981 cagtcattca tctaggccag caatcgctca ctggctcaag cagcctaccc gggttcagta
     2041 cgggccgtac cttatgaacc cctatttggc cttgctccgg gtggagttta ccgtgccacg
     2101 gactgttacc agccgcgcgg tgcgctctta ccgcaccctt tcacccttac ctgatcccgc
     2161 ttgcgcgggc catcggcggt ttgctctctg ttgcactggt cgtgggtttc ccccccaggc
     2221 gttacctggc accctgccct atggagcccg gactttcctc ccctccgccc gtctcccccg
     2281 aagaggacga cgacgaagcg gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt
     2341 tgcgcgccct tgtcaccccg cgttgcgcat tccaatcgcc agtgtcgcgg cgatattacg
     2401 tgaagcacgg cagatattgt cataagcccc gcggaatgct tcgtccaacg tacctatgct
     2461 ggtcaatacg ctgaagaccg catcaatgcc atgctgatgt acaacgccaa catcatcggt
     2521 caggctaccc gcaatgccaa tcaccggttt atggtacttc ttcgccacgt ttgcgacacc
     2581 aatcggtacc ttgccgtgaa tactctggct gtcaatacgc ccttcaccgg tgatcaccag
     2641 cgtacaatcg tgaatatgtt cttccagatt cagcgccgta gtgacgattt caataccact
     2701 tttcagttcc gcaccaagaa acgccatcag cgccgcgccc ataccgcccg cagctcctgc
     2761 gccggggaca tctttcacat caacatgcag cgctttttta atgacctcgg catagtgaga
     2821 gaggttattg tccagctcaa caatcatcgc ttcactggct cccttttgtg ggccaaagat
     2881 acgcgatgcg ccgttatcgc ccaccagcgg attggtgaca tcacaagcga cgcgaatgac
     2941 gcaatctttt aagcgcggat cgaggccgga aatatcaata tcattcagag tattaagact
     3001 accgccgcca aaaccaattt cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg
     3061 taccatgcct gcgccgccat catttgtagc gctgccgcca atgccgataa taatgtttgt
     3121 cgcaccgctc tccagcgcct gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag
     3181 tggatcgcgt ttttccgcag gtaccagctc cagcccactg gccgccgcca tttcaataaa
     3241 cgcggttttg ccatcgccgg agatccccca actggcattc actttctcgc ccaacggccc
     3301 tgtaacccag gcgtgacgtt cggaaccctg ggtggctgca atcatcgctt ccaccgttcc
     3361 ttcgccaccg tcggcaaccg gaacagaaac gtactgtgca tcaggaaaaa tttcccgaaa
     3421 tcctttttct atcgcctgcg caacctcgct ggcagataaa ctttctttat aagagtctgg
     3481 ggcgattacg attttcatac ctatgcctgt taccacatga cgccggaggg cgtttctctt
     3541 attcggcgtg gattccaggc ccggattgca atacgccatc cgggcacgac gtcattaacg
     3601 agtaacttcg actttcgcca gtttttcgta gtagcacgcc agagcgctat gatccgccgt
     3661 tcctaaacca tctgcacgca gtgcctgcat catctccata accgcagctg tgagcggcag
     3721 ttgcgcgccg acgccgtgag aagtatccag cgcattcgcc agatccttaa tatgcagatc
     3781 aatgcggaag cccggcttga agttgcggtc catcaccatc ggcgctttgg catccagcac
     3841 ggtactgccc gccagcccac cgcgaattgc ctgataaacc aggtccgggt taacgcccgc
     3901 tttagttgcc agcgttaacg cttctgacat cgcagcaata ttcagcgcca caatgacctg
     3961 atttgccagt ttggtgacgt tacctgcacc gatttccccg gtatgcacca cggaacccgc
     4021 catcgctttc atcaaatcat agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac
     4081 tgacagcgta ccgtcgatgg ctttcggttc accgccgctc accggagcat ccagcatatc
     4141 aatgcctttc gctttcagcg cttcgctgat ttcacggctt gccagcggtg cgatagaact
     4201 catatcgatc aataccgtac ctggcttcgc gccttcaata atgccattct cacccagcgc
     4261 cacctctttc acatgagggg agtttggcag catggttatg atgacgtcgc actgttcagc
     4321 gatcgcttta gccgtagacg ctgtttctgc acctgcagca atcacgtcag caatagcttc
     4381 tgggttacgg tcagcaacca ccagcgagta acctgctttc agaaggtttt tactcattgg
     4441 tttacccata atccccaggc caataaaacc aactttcata gtcatatcaa tcatctctct
     4501 tgttgcggtg gtggttattt tttaaaggta tcagccagtt tctgagtggc agagcggaag
     4561 acgccaagat cgctgccgac agccacaaac gtcgcgcccc attccagata acgacgcgca
     4621 tcggcttcga ccggcgcgag aataccgctg ggtttgccgt gcgcactggc acggttaaaa
     4681 atatgctgaa ttgctttttg tacatccgga tgcgaggcat tgccgagatg gcccaatgcc
     4741 gcagccagat cgctggggcc gacgaagata ccgtctacac cttcggtagc ggcaatagca
     4801 tcgacgttat ctacgccctg ctgactttct atctggacca gaatagtgat gttcttgttc
     4861 gactgagcga aataatccgc cacggtgcca aacatattgg cgcggtgaga aacggagacg
     4921 ccgcgaatgc cttccggcgg gtaacgagtt gatgccaccg cctgctctgc ttcctctttt
     4981 gtttctacaa aaggaatcag gaagttatag aaaccgatat ccagaagacg cttaataatt
     5041 accggctcgt tggtcggcac tcgcactact ggcgcgctgg cgctgccttt cagggccatt
     5101 aactgcggaa taaacgtgga aatatcgttt ggcgcatgtt cgccatccag caccagccag
     5161 tcaaacccag ccaaaccaag aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa
     5221 ccaatttgta cctgtttcgc agccagtgcg gctttgaatt tattcgggaa aacatcgtta
     5281 ttcatcgctt atacctttgc ttatttctgc aattccatac gtttaatgtc gccaactacg
     5341 aagaggtagc agaccatcgc catcagcgct gaacatccca cgaaaaccag tgctgcattg
     5401 aaggagtgca gttcacttac caggtagcca atcaccagtg gagtgacaat ggaggcaaca
     5461 ttgccaaaga cgttaaagac gccgccacag aggccaacaa tctctttcgg cgcggtgtca
     5521 gaaatcaccg gccagcccag agcaccaaat cctttgccaa aaaaagccag cgccatcagc
     5581 atgaccacca gcgtggtgtt gttggtgtag ttacataaga tgatggtgga agccagcaac
     5641 attcccagca caatcggtag cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga
     5701 taatccgaga agacacctcc cagcacgccg cccgcaaaac cacacagtgc cggaatcgag
     5761 gcgaccagac ccactttcag aatcgacatg cctttttcct gcaccagata aatcgggaac
     5821 caggtgagga agaaccaggt gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac
     5881 atcatgcggt tagagagcaa ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg
     5941 ctgcccggct ttttgtggtc catatcgacc accgcgccat tttcagagat aaacttcagc
     6001 tcttccgcag acatacgtgg gtgatctgtc gggttataaa tcaacttgat ccacagcgcc
     6061 gtcagcacaa aaccaatcac ccccataacg gtgaagacgt gctcccagcc ccaggcgaaa
     6121 gtcagccagc caagcagagg cgaaaagagc gccagcgaga aatattgcgc cgagttaaag
     6181 atggcggagg cagtaccacg ttctttcgtc gggaaccagg cggcgacaat acgggcgttc
     6241 gccgggaatg atggcgcttc cgagaagccg agcataaagc gcataaagaa catagagatc
     6301 cctgcccagg ccagcgggaa catatcaaca aagccttgca ggaaggtgaa tagcgaccag
     6361 aaaaagaggc tgtaggtgta aacttttttc gagccaaact tatcaagcag ccagccgccg
     6421 ggaatttgca tcagcaggta ggcccagcca aaagcggaga agatgtaacc catcgaaacc
     6481 gtacttaact gcaactcttt tgccacttcg gtaccagcaa tagacagcgt tgcacaatcg
     6541 gcgtagttaa cggcggtaac aataaaaata atcagtaata ttaaatagcg ggtatgcacg
     6601 cctttctttt ttacgtcaac ggtgt
//
LOCUS       DADLTD010000206         6584 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLTD010000206 DADLTD010000000
VERSION     DADLTD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.208"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            18..173
                     /locus_tag="JN701_003497"
                     /pseudo
     CDS             18..173
                     /locus_tag="JN701_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
     gene            166..1947
                     /locus_tag="JN701_003498"
     CDS             166..1947
                     /locus_tag="JN701_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7365816.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            2128..2466
                     /gene="glnK"
                     /locus_tag="JN701_003499"
     CDS             2128..2466
                     /gene="glnK"
                     /locus_tag="JN701_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY7365817.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            2496..3776
                     /gene="amtB"
                     /locus_tag="JN701_003500"
     CDS             2496..3776
                     /gene="amtB"
                     /locus_tag="JN701_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY7365818.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTFALVCILWVVYGYSLAFGEGNNFFGNVNWLM
                     LKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTLSY
                     IPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPHNL
                     PMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRGKP
                     SLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPCDV
                     FGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVAFI
                     GYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(3825..4685)
                     /gene="tesB"
                     /locus_tag="JN701_003501"
     CDS             complement(3825..4685)
                     /gene="tesB"
                     /locus_tag="JN701_003501"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY7365819.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            4903..5475
                     /locus_tag="JN701_003502"
     CDS             4903..5475
                     /locus_tag="JN701_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7365820.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(5506..5817)
                     /locus_tag="JN701_003503"
     CDS             complement(5506..5817)
                     /locus_tag="JN701_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY7365821.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            5980..6076
                     /gene="ffs"
                     /locus_tag="JN701_003504"
     ncRNA           5980..6076
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JN701_003504"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6196..6549
                     /locus_tag="JN701_003505"
     CDS             6196..6549
                     /locus_tag="JN701_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY7365822.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
BASE COUNT         1443 a         1635 c         1913 g         1593 t
ORIGIN      
        1 gggatgcgtt catataaatt attgtgatgc agcacggaca tatcgcccag cgtggcaatc
       61 atgatgagct ggcgcaacaa agcggctggt atcgcgatat gtatcgctat caacaactgg
      121 aggcggcgct cgacgacgct ccggaaattc gcgaggaggc cgtcgatgcg tagttttagc
      181 caactgtggc cgactctcaa gcgtctgtta gcgtacggtt cgccgtggcg taaaccgctg
      241 gggattgcgg tcctgatgat gtgggttgcg gcagcggcag aagtcagtgg gccgctgctt
      301 atcagctatt ttatcgacaa tatggtagcg aaaaataacc tgccgttgaa aatggttgca
      361 gggctggctg cggcgtatgt tgggctacaa ctgtttgccg ccgggctaca ttacgcgcag
      421 tcgctgctgt ttaatcgggc ggcagtaggc gtagtgcaac agttgcgtac cgacgtgatg
      481 gatgccgcat tgcgccagcc attaagcgag tttgataccc aacccgtcgg gcaggtgatt
      541 tcccgcgtca ctaatgacac tgaagtgatc cgcgatctct acgttaccgt agtggcaact
      601 gtcctgcgta gcgccgcact ggtgggcgcg atgctggtgg cgatgttcag cctcgactgg
      661 cgaatggcgc tggtggcgat aatgattttc ccggtggtgc tggtggtaat ggtgatatac
      721 cagcgttaca gcacgccgat tgtccgtcgt gtgcgcgcct atctggcgga tatcaacgac
      781 ggctttaacg aaatcatcaa tggcatgagc gttatccagc agttccgtca gcaggcgcga
      841 tttggcgaac gtatggggga ggccagtcgt tcacactata tggccaggat gcaaaccctg
      901 cgcctcgacg gttttctgct gcgtccgctg ctgagtctgt tttcatcgct cattctttgt
      961 ggcttgttga tgctgtttgg cttctccgct agcggcacca ttgaagtagg cgtgctgtat
     1021 gcgtttatca gctatcttgg gcgacttaac gaacccttaa tcgaactgac cacgcaacag
     1081 gcgatgctgc aacaggctgt tgttgctggt gagcgcgtgt ttgaactgat ggacggaccg
     1141 cgccagcaat atggcaatga tgatcgcccg ttacagagtg gcaccatcga agtcgataac
     1201 gtgtcatttg cttatcgcga tgacaatctg gtgctaaaga acattaatct ctctgtgcct
     1261 tcgcgtaact ttgtggcact ggtcgggcat accggcagtg gcaaaagcac cctcgccagt
     1321 ttattgatgg gctattaccc gctaacggaa ggtgagattc gccttgatgg tcgtccatta
     1381 agttcgctaa gtcacagcgc gctgcgccag ggcgtggcaa tggtgcagca agatccggtg
     1441 gtgctggcgg ataccttcct cgccaacgtg acgctggggc gtgatatctc cgaagaacgc
     1501 gtctggcagg cgctggaaac cgtgcaactg gcggagctgg cgcgcagcat gagtgatggt
     1561 atttatacgc cgctgggcga gcaggggaat aatctctccg tcgggcaaaa gcaactgctg
     1621 gcactggcac gcgtgttggt ggagacgccg caaatcctga tccttgatga ggcaaccgcc
     1681 agcattgact ccggtactga acaggcgatt caacatgctc tggcggctgt gcgtgaacat
     1741 accacgctgg tggtgattgc tcaccgctta tcgaccattg ttgatgccga caccattctg
     1801 gtgcttcatc gtgggcaagc cgtggagcag ggcactcacc agcaactgct ggcggcccag
     1861 ggacgctact ggcagatgta tcaactgcaa cttgcgggcg aagagctggc agccagcgtg
     1921 cgtgaagaag aatcattgag cgcctgaata gcgcaatatt tcatcgttgg tgcaaaaatg
     1981 taacgcactg tgcactgtca tagtgcgttt tcattttcaa tcttcttaac ttcctgctct
     2041 ctttctcgtt tttcatttct ggcacaccgc ttgcaatacc ttcttcgtgt agcagaacca
     2101 ttaccgaatt ctgactggag gggatctatg aagctggtga ccgtgataat caaaccattc
     2161 aagctggaag acgttcgtga agcgttatct tccattggta ttcagggcct gaccgtcacc
     2221 gaagtgaaag gtttcgggcg tcagaaaggg catgccgagc tgtaccgggg ggcggaatac
     2281 agcgtcaact tcctgccaaa agtaaaaatt gatgtggcaa ttgctgatga ccaactcgat
     2341 gaagtgatcg atatcgtcag taaggcggct tacaccggaa aaattggcga cggcaaaatc
     2401 ttcgtcgctg aattgcaacg cgtcattcgt attcgtaccg gcgaagccga cgaagcggcg
     2461 ctgtaatctc tggcacacag caacaggaac gaaaaatgaa gatagcgacg ataaaaactg
     2521 ggcttgcttc actggcgatg ctgccgggac tggtaatggc tgcacctgcg gtggccgata
     2581 aagccgacaa tgcgtttatg atgatttgta ctgcgctggt gctgtttatg actattccgg
     2641 ggattgccct gttttacggt gggttgattc gcggcaaaaa cgtgctgtcg atgctgatgc
     2701 aggtgacgtt tgcactggtc tgtattctct gggtggttta cggttactcg ctggcgtttg
     2761 gtgagggtaa caacttcttc ggcaacgtta actggttgat gctgaaaaac atcgaactga
     2821 cggcggtgat gggcagcatt tatcagtata tccacgtagc gtttcaggga tcgtttgcct
     2881 gcattaccgt aggcttgata gttggggcgc tggcggaacg aatccgcttc tcagctgtgt
     2941 tgattttcgt ggtggtatgg ctgacgctct cttacattcc gattgcgcat atggtgtggg
     3001 gcggcggttt gctggcttct cacggtgcac tggatttcgc gggtggcaca gtggtgcaca
     3061 ttaacgccgc aatcgccggt ctggtgggcg cgtatctgat aggcaaacgc gtgggcttcg
     3121 gtaaagaggc gtttaaaccg cacaacctgc cgatggtctt cactgggact gccattctct
     3181 atatcggttg gtttggcttt aacgccgggt cagcgggcac ggcgaatgaa atcgcggcac
     3241 tggcatttgt gaatactgtg gtcgcaacgg cggcggcaat tcttggctgg atcttcggtg
     3301 aatgggcgct gcgtggtaag ccttcactgc tgggggcgtg ttctggcgcg attgccggtc
     3361 tggtcggcgt gacgccagcg tgcggctaca ttggggttgg cggcgcgttg attatcggcg
     3421 tggtagctgg tctggcgggc ttgtggggcg ttaccatgct caaacgcttg ctgcgggtgg
     3481 atgatccctg cgatgtcttc ggtgtgcacg gcgtttgtgg cattgtcggc tgtatcatga
     3541 ccgggatttt tgccgccagc tcgctgggcg gcgtgggctt cgctgaaggt gtgacgatgg
     3601 gtcatcagtt gctggtacag ctggaaagca tcgccattac gatcgtctgg tccggtgtgg
     3661 tggcatttat tggctacaaa ttggcggatc tgacggttgg tctgcgtgta ccggaagagc
     3721 aggagctaga agggctggat gtcaacagtc acggcgagaa tgcctataac gcgtaacaag
     3781 cactgcaaaa aacagccgga cggttttcac ctccggctat ttttttaatt gtgattacgc
     3841 atcacccctt cctgaacggt cgaggcaacc agtacgccgt cctgggtata aaactcaccg
     3901 cgcacaaagc cacgtgcgct ggacgccgag gtgctctcca cgctatacag cagccattca
     3961 ttcaaattaa acgggcgatg gaaccacatg gaatggtcaa tggtggcaat ctgaatcccc
     4021 ggttcgagaa aaccgatgcc gtgcggctgt agagctaccg gcaggaagtt aagatcagaa
     4081 gcgtaaccga gcagatactg atgaacgcgc aggtcatccg gcacgctacc atttgcgcgg
     4141 atccacacct gacgatgtgg ttctgcgacg tgacctttca gtgggttatg aaactccacc
     4201 ggacggactt ccagcggacg atcgcagatg aatttatctt taagcactgg cggcagcagg
     4261 tgcgccagcg attgggcgat ttgcgtttcc gaaggcaggc catcaggcgc tggcgcggac
     4321 ggcattgttt tttgatgttc gaaacccgct tctggtgcct ggaaagaggc cgtcatataa
     4381 aaaatcggtt tgccgttttg aatagcagca acccggcggg cgctgaagct gttaccatca
     4441 cgcagcgttt cgacatcata aataatcggt ttcttactat cgcctgggcg aagaaagtag
     4501 ctgtgaaacg aatgtaccag ccgctcttcc gggacggtct cttttgcagc atacaaggcc
     4561 tgacccacga cctggccgcc aaacacctgg cgtaaaccta aatcttcact ctggccgcga
     4621 aagagtcctg cctcaatttt ttccagattt aacaatgcca gtaaattttt tagcgcctga
     4681 ctcatataac tctccagtaa caaagctgcc gcagcaagcc aaagtgagtt gagtataacg
     4741 caaatttgcc actggtccga tgggtgcaat aatctgaatt acgggctaat tacaggcaga
     4801 aatgcgtgat gtgtgccaca cttattgacg ttactatttt gttaaccact cttccggcga
     4861 ggaaagttag cccgctggtg cattgataat aaggagaaat gaatgaaact cgtgcacatg
     4921 gccagtggtt tagcggttgc gattgcgttg gcggcttgcg cagataaaag cgcggatatt
     4981 cagacgccag ccccggctgc aaatacgtct atttcagcaa cacaacaacc tgctatccag
     5041 caaccgaatg tctccggtac cgtctggatc cggcagaaag tcgcactgcc gcctgatgct
     5101 gtgctgaccg tgacactttc tgacgcgtcg ttagccgatg caccgtcaaa agtgctggcg
     5161 cagaaagcgg tgcgtaccga aggtaaacag tcaccattca gctttgttct gccatttaac
     5221 ccggcagatg ttcagccgaa cgcgcgtatt ctgttgagtg cggcgattac cgtgaatgac
     5281 aaactggtat ttattaccga taccgttcag ccggtgatca acaagggcgg aactaaagcc
     5341 gacctgacat tggtgccggt gcagcaaacc gccgtgccgg ttcaggccag cggtggtgca
     5401 acgactaccg taccttcgac ttcaccaact caggtgaatc cgtcttcggc agttccagct
     5461 cctacgcaat attaagcgac gttaaccctc tccgcccgga gagggttagt agttccagcg
     5521 ataacgctgc aagtcgattt gcccgcttcc cgataccatc acaccttctg ccagtaatgc
     5581 ctgtcgctga cgctgtaaat ccggtccggt tagcgaaatt gtgccgtggc gattaaccac
     5641 ccggtgccag ggtaaggtgc tgccttcagg gagacgcttt aacacaccgc ccacctggcg
     5701 cgcggcgcgg ggcgatcccg ccagtttcgc cacatcaccg taagtggtga catagccttc
     5761 gggaatagcg gcgacgattt gccagacgcg ttggggaaat gaatcttctt tttccatctt
     5821 ttcttcctga ggtaattttt cagcataatc tggaaaaacg cccgagtgaa gtcgcattgc
     5881 gcaagaaacc agcatctggc acgcgatggg ttgcaattag ccggggcagc agtgataatg
     5941 tgcctgcgcg ttggttctca acgctctcaa tgggggctct gttggttctc ccgcaacgct
     6001 actctgttta ccaggtcagg tccggaagga agcagccaag gcagatgacg cgtgtgccgg
     6061 gatgtagctg gcagggcccc cacccatttc tgcctcccac cgtttcgtca aaaaaaacca
     6121 acatggctaa actttaacca caactgacgt cgcaagaatt gtctggctgc gcagtacgct
     6181 tcggaggtat gtctgatgaa gtatgttgat ggttttgtgg ttgccgttcc tgccgataaa
     6241 aaggatgcct atcgggaaat ggccgctaag gctgcgccat tgtttaaaga gtttggcgcg
     6301 cttcgtattg tcgaatgctg ggccagcgat gtaccggatg gcaaagtgac cgattttcgt
     6361 atggcggtga aagcggaaga gaatgaagag gttgtcttta gctggattga atacccttca
     6421 aaagaggtcc gcgacgctgc taatcaaaag atgatgtcgg acccacggat gaaagagttc
     6481 ggcgagtcca tgccgtttga cggcaagcga atgatctatg gcggattcga gtcaatcatc
     6541 gacgaatagg tagcgtgacg ggctgtgcgg cgtgccgtca gccc
//
LOCUS       DADLTD010000207         6405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLTD010000207 DADLTD010000000
VERSION     DADLTD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.209"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            453..3638
                     /gene="rne"
                     /locus_tag="JN701_003506"
     CDS             453..3638
                     /gene="rne"
                     /locus_tag="JN701_003506"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY7365823.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVTEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3835..4788)
                     /gene="flgL"
                     /locus_tag="JN701_003507"
     CDS             complement(3835..4788)
                     /gene="flgL"
                     /locus_tag="JN701_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY7365824.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNLLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4800..>6405)
                     /gene="flgK"
                     /locus_tag="JN701_003508"
     CDS             complement(4800..>6405)
                     /gene="flgK"
                     /locus_tag="JN701_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAY7365825.1"
                     /translation="AAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNG
                     VYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFT
                     SLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYA
                     KQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMAN
                     GYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQ
                     DLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPAVLQNTKNKGDVAI
                     GATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTP
                     AVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQALLDLQSNSKTVG
                     GAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSISGVNLDEEYGNLQR
                     FQQYYLANAQVLQTANAIFDALINIR"
BASE COUNT         1543 a         1735 c         1662 g         1465 t
ORIGIN      
        1 ggatgcgttc atataaaaac tgtgagtaag cgggtgataa atggtaaaag tcatcttgct
       61 ataacaaggc ttgcagtgga ataatgaagc cgtttccgtg tccatccttg ttaaaacaag
      121 aaattttacg gaatacccca ttttgcccga ccgatcatcc acgcagcaat ggcgtaagac
      181 gtattgatct ttcaggcagt tagcgggctg cgggttgcag tccttaccgg tagatggaaa
      241 tatttctgga gagtaatacc cagtctgttt ctttgataat tgcgctgttt ttccgcatga
      301 aaaacgggca accgacactc tgcgcctctt tgagctgacg ataaccgtga ggttggcgac
      361 gcgactagac acgaggccat cggttcacac ccggaaaggc gttactttgc ccgcagctta
      421 gtcgtcaatg taagaataat gagtaagtta cgatgaaaag aatgttaatc aacgcaactc
      481 agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg tctgtatgac ctggatatcg
      541 aaagtccagg gcacgagcag aaaaaggcaa acatctacaa aggtaaaatc acccgcattg
      601 aaccgagtct ggaagctgct tttgttgatt acggcgctga acgtcacggt ttcctcccac
      661 taaaagaaat tgcccgcgaa tatttccctg ctaactacag tgctcatggt cgtcccaaca
      721 ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca gatcgataaa gaagagcgcg
      781 gcaacaaagg cgcggcatta accaccttta tcagtctggc gggtagctat ctggttctga
      841 tgccgaacaa cccgcgcgcg ggtggcattt ctcgccgtat cgaaggcgac gaccgtaccg
      901 aattaaaaga agcactggca agccttgaac tgccggaagg catggggctt atcgtgcgca
      961 ccgctggcgt cggcaaatct gctgaggcgc tgcaatggga tttaagcttc cgtctgaaac
     1021 actgggaagc catcaaaaaa gccgctgaaa gccgcccggc cccgttcctg attcatcagg
     1081 agagcaacgt aatcgttcgc gctttccgcg attacttacg ccaggacatc ggcgaaatcc
     1141 ttatcgataa cccgaaagtg ctcgaactgg cacgtcagca tatcgctgca ttaggtcgcc
     1201 cggatttcag cagcaaaatc aaactgtaca ccggcgagat cccgctgttc agccactacc
     1261 agatcgagtc gcagatcgag tccgccttcc agcgtgaagt ccgtctgccg tccggtggtt
     1321 cgattgttat cgacagtacc gaagcgttaa ctgccatcga catcaactcc gcacgcgcga
     1381 cccgcggcgg cgatatcgaa gaaaccgcgt ttaacaccaa cctcgaagct gccgatgaga
     1441 ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat tgttatcgac ttcatcgaca
     1501 tgacgcctgt acgccaccag cgtgcggtag aaaaccgtct gcgtgaagcg gtgcgtcagg
     1561 accgtgcgcg tattcaaatc agccatattt ctcgctttgg cctgctggaa atgtcccgtc
     1621 agcgcctgag cccatcactg ggtgaatcca gtcatcacgt ttgtccgcgt tgttctggta
     1681 ctggcaccgt gcgtgacaac gaatcgctgt cgctctctat tctgcgtctg atcgaagaag
     1741 aagcgctgaa agagaacacc caggaagttc acgccattgt tcctgtacca atcgcttctt
     1801 acctgctgaa tgaaaaacgt tctgcggtaa atgccattga aactcgtcag gacggtgtgc
     1861 gctgcgtgat tgtgccaaac gatcagatgg aaaccccgca ctaccacgtg ctgcgcgtgc
     1921 gtaaagggga agaaacccca accttaagct acatgctgcc gaagctgcat gaagaagcga
     1981 tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg tccggaacaa cctgcgctgg
     2041 caacctttgc catgccggat gtgccgccag cgccaacgcc agctgaacct gccgcgcctg
     2101 ttgtagctcc agcaccgaaa gctgcaccgg caacaccagc agctcctgca caacctgggc
     2161 tgttgagccg cttcttcggc gcactgaaag cgctgttcag cggtagtgaa gaaaccaaac
     2221 cgaccgagca accagcaccg aaagcagaag cgaaaccgga acgtcaacag gatcgtcgca
     2281 agcctcgtca gaacaaccgc cgtgaccgta atgagcgccg cgacacccgt agtgaacgta
     2341 ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg tcgccaggca cagcagcaga
     2401 ctgccgagac gcgtgagagc cgtcagcaag ctgaggtaac ggaaaaagca cgtaccaccg
     2461 acgagcagca agcgccgcgt cgtgaacgta gccgccgccg taacgatgat aaacgtcagg
     2521 cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc tgttcaggaa accgaacagg
     2581 aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg tcagctcaat cagaaagtgc
     2641 gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc ggtggttgaa gaaactgccg
     2701 ctgccgaacc aattgttcag gaagctccag ctccacgcac agaactggtg aaagtcccgc
     2761 tgccagtcgt agcgcaaact gcaccagaac agcaagaaga gaacaatgct gataaccgtg
     2821 acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg tcacctgcgc gtgagtggtc
     2881 agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca gtcgccaatg ccgttgaccg
     2941 tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg gatccgctat ccaattgtac
     3001 gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga agaagtacaa gtgcagccga
     3061 tggtgactga ggtccctgtc gccgccgctg tcgaaccggt tgttagcgcg ccagttgttg
     3121 aagaaatggc tgaagtcgta gaagcccccg taccggttgc cgaaccgcaa ccggaagtgg
     3181 ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac tgaacagccg caggtgatta
     3241 ccgagtctga tgttgccgta gcccaggaag ttgcagaaca cgcagaaccg gtggttgaac
     3301 cgcaggaaga gacggcagat attgaagaag ttgccgaaac tgctgaggtt gtggttacag
     3361 aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc tgaagtcgca gcagaagttg
     3421 aaacggtagc cgcggttgaa ccagagatca ccgttgagca taaccacgct accgcgccaa
     3481 tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc gcatcacagt gactggcagc
     3541 gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg tcatacggca acgcatcatg
     3601 cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata attagctcaa agtaatcaag
     3661 ccctggtaac tgccagggct tttttatttc atgtttgaat caacatatta aaattcatca
     3721 aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa aaaatgcccg ccccggcgat
     3781 ccctgagcgg gcagacaaaa catacccagt ttcatgatat gttttaaagc gagcttattt
     3841 gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg atgcctgcaa
     3901 tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat caaccagatc
     3961 gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct aaggaatcca gcgactccag
     4021 ttcgttcagc tgcgtgccta attccgcgcg aacggcaagg acattattca gcaagttttt
     4081 cagtccacgg ttggttttat ccaatgccgc agcggcgatt tctttatccg cttcgctatc
     4141 cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa gattggtttc
     4201 agaagcgcta ccgtctggct ccgcaactgc gttgctggta atactgtcga aaattttgtc
     4261 acccgtgtgc cctatcacca tcgaacgcga agcatcgacc tgttgcttaa tactttctgc
     4321 tccaccgacg tatttccctt tctcttcgct aaacggcgca gtctctgttt tataaccggc
     4381 aaaaatgtag cgcccgttac cgtcagtggt gttcgccaga ttcagcaact ggtcacgaag
     4441 cccctgaata tccgtagcca gcgaggcccg gtcatcgtca ctcaaggtgc cattgctggc
     4501 gtagacaatt ttttcctgag cattctggat agcagtggtg acctggctaa gtacactctc
     4561 ttccagtgac actttttgaa tggcgaaagt acgcgccagc gtgtactggc tgttttgcgc
     4621 ctgtgcctgg gagagaacta cggcttgtga tgctgcaatg ggatcgtcag aagggttaac
     4681 gactcgctta cctgtcgaca tctgctcgcc gtacttcatc cattccgcct gagaattggt
     4741 gatgccacgc atgttttgct ggtacatcat ctgggtactg aaacgcatct tatctcccct
     4801 tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg tctgcagaac ctgcgcattc
     4861 gccaggtaat actgctgaaa acgttgcaga tttccgtact cttcgtcgag attgacgcca
     4921 gagatagact gctgctgatt ggaaagctgc gtcaccacat taccttgcgt ggcgctactg
     4981 gttttcaacg tcgcggtttt attaccgata tcactcacta acgaggcata agcgtcgtta
     5041 aaggatttcg caccgcccac cgttttactg ttgctttgca gatccagcag ggcctgaccg
     5101 ttgcggttat cgctatcacc cgcatctttt tcgctcgcca tcgctatttt cgcttcgtcg
     5161 gtgattaata catccatgtt gacgatggcg tcacttactg gtttcagcgt gaagctgtcg
     5221 ttaacggcag gcgttcctgt aaacgtcaac tccagaccat caaatgccac tttaccgttg
     5281 gcatccggcg tcaccgtaaa agtggtattg ctggcaaggc gggtgacctg ccactgatta
     5341 ttatcgaacg agattttgta atctgtcgcc agtaccgcgg aggcatcagt taccgtggca
     5401 ccgatcgcaa cgtcaccttt gtttttcgtg ttttgcaaaa ccgcgggctt accgatagca
     5461 aagaaatctt caccagcatc gccgttggca tcaaatccgg ctttgtgttg ggtgttgaaa
     5521 gcctcggcaa atgccagcgc cagttgtcca agcgtattac gcgtctggtc cagatcctga
     5581 gaacggaatg tcagaatgcc gcccagcgac ccggtattca gtaatttctc cgggatctca
     5641 atattgcctg ccgtcccatc aacgtaagcg acagtcgtac gagaagggtc ggcgctggaa
     5701 ggaactgccg ccagttgccg cgccgtactt ccctgaacca gtgagtaacc attggccatc
     5761 gtgatgttat aagtgccgcc atcctgaacg ctgacttcta caccaacaat ctggtttaat
     5821 tcgctcacca gttgatcgcg ttgatccagc agattgttag gtgacgcccc tgcccccacg
     5881 cctgtcaggc gggagatttg gtcgttcagg ctggcgattt gtttagcgta attgttgatc
     5941 tgatcaacgc tggcacctat cgcgatattg acctgtttgt cctggtcgcg aagatactga
     6001 tcggtggttt taaactgatt caccaaccct tctgatttcc caatcagcgc ctggcgcgct
     6061 gccgggtctt ccgcgttact caccagcgtt tgcaggctgg tgaagaaatc ctgcatctgt
     6121 gttgccagcg aagaggtact ggtggagagc atattgtcga ttttcgacat ctgctcatag
     6181 cgggcagtca ggccgctact ttgcgtctgc gccgcacgta actggttggt gataaacgca
     6241 tcatactcac gctgcacacc agaaacgtag acaccattgc caacccagcc gccagcgccc
     6301 aacgtgctat tggcctgcgc cataatagtg gtttggcggg tatatccggc aacgttatag
     6361 ctggagatat tattacttgc cgtatttaac gccgcctggg ccgcg
//
LOCUS       DADLTD010000208         6397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLTD010000208 DADLTD010000000
VERSION     DADLTD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.210"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..210)
                     /locus_tag="JN701_003509"
     CDS             complement(<1..210)
                     /locus_tag="JN701_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HAY7365826.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNS"
     gene            689..2467
                     /locus_tag="JN701_003510"
     CDS             689..2467
                     /locus_tag="JN701_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HAY7365827.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(2507..2986)
                     /gene="xdhC"
                     /locus_tag="JN701_003511"
     CDS             complement(2507..2986)
                     /gene="xdhC"
                     /locus_tag="JN701_003511"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAY7365828.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2983..3861)
                     /gene="xdhB"
                     /locus_tag="JN701_003512"
     CDS             complement(2983..3861)
                     /gene="xdhB"
                     /locus_tag="JN701_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY7365829.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3872..6169)
                     /gene="xdhA"
                     /locus_tag="JN701_003513"
     CDS             complement(3872..6169)
                     /gene="xdhA"
                     /locus_tag="JN701_003513"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAY7365830.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1596 a         1511 c         1618 g         1672 t
ORIGIN      
        1 ggagttgtcg cggaatacgg aaatacctaa tccactatta aagactttgg ttgagatgtt
       61 ttcagcacgc agtgctttta atgctgcggc aacgtccagt acttgtttca gttcatctgg
      121 cgtctgttcc cacgttaaca aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat
      181 atccttaatc agctcattaa cagttttcat cataaagtcc cggtagtgga atgacaggca
      241 aagtggttta tttcactgtg acttgccaaa gtgattggtg ttttcacagc tatacctggg
      301 actaacaata agtgtgccag aattatccgc gcggctgcaa aagactaatt tgacggataa
      361 tgtgtggggt tgatcacaaa ttgatgccag cttctggttg tttgtaaaat acgaaaacgt
      421 ttggcttttt ttatcgcgtt atttatttgc tgctaagtat cagaaataag ataaaggggc
      481 aggctatctc tcttcagttt gataaactca ccgccgtaat tctcatattg atatagaaat
      541 gtgatatctc ttccataatt tcattagaaa atgataaaaa tcttatgttt ttatcaaagc
      601 caatttcgcc tgagttaata tcatcatgat ctgtgaacca tcaacgtctt caatgatcga
      661 cttttaagta agtctggaag gtaaagctat ggagcttgct actacgcagt cagtattgat
      721 gcaaattcaa ccgacaattc agcgttttgc cagaatgctt gccagcgttt tgcagcttga
      781 ggttgagatc gttgatgaaa acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt
      841 tcttggtcgc cagttgagcg gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac
      901 tgaaaaagtt gtgacacagt ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga
      961 aaattgccgt gaaaaagcat ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg
     1021 ggtgataagt ttgattgccg ttacccacga gcaacaagag catatcagtg ataatttacg
     1081 cgaattttct gattatgttc gccatatatc caccattttt gtttcgaaac ttctggagga
     1141 tcaggggcca ggagataaca tcagtaaaat attcgcgacc atgatcgata atatggatca
     1201 gggcgtatta gttgttgatg atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa
     1261 aacacttggt gttgtacaaa ataatattat tgggaaacct atccgtttca gaccattaac
     1321 atttgagagt aattttactc atggacatat gcagcatatt gtttcgtggg acgataaaag
     1381 tgaattaatc attggtcaat tgcataacat tcagggccga caattatttt taatggcatt
     1441 tcaccaatcg cataccagtt tttctgtagc aaatgcacct gatgaaccac atattgaaca
     1501 attggttggc gagtgccgtg ttatgcggca attaaaacga ctcattagcc gtattgcacc
     1561 cagcccatcc agcgttatgg tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg
     1621 agcaatccat aagttgagcg gaagacggaa taaacccttt attgctatca actgtgccgc
     1681 gattccggag cagcttctgg aaagcgaact gttcggttat gttaaaggcg catttactgg
     1741 cgcttctgcc aacggtaaaa cagggttgat tcaggcggcg aatacgggca cgctgtttct
     1801 cgatgaaata ggtgatatgc cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc
     1861 gcgtgaaatt ctgccgattg gtgccagtag cccaatacaa gtcgacattc gcatcatttc
     1921 tgcaactaat cagaatttgg cccagttcat tgccgaaggt aaattccgcg aagatctctt
     1981 ctaccgactt aatgttatcc cgataactct gccaccgctg cgtgaacgtc aggaagatat
     2041 tgaactattg gtgcattact ttttacatct gcatacccgt cgtctgggat cggtttatcc
     2101 tggcattgct cccgatgtcg tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg
     2161 cgagttaagc aatttgatgg aatatctggt taacgtagtt ccttcaggtg aagttatcga
     2221 cagcacgcta ttgccgccaa atctgctgaa taatggcaca acggagcaaa gtgatgtaac
     2281 agaggtcagt gaggcgcacc tgtcactcga tgatgcgggc ggcacggcgc tggaggagat
     2341 ggaaaagcaa atgatccgcg aggcgctttc acgtcataac agcaagaagc aagttgctga
     2401 tgaactgggc atcggcattg ctacgctcta tcgcaagatt aagaaatatg agttgttaaa
     2461 cacataacct cagtgaatcc ggcctgaatt caggccggat atccttttac ttcgttttct
     2521 cgcaatccag aactgtattt acaatcatct gataccccgt gcagcgacaa agatttcccg
     2581 ccagtccgcg acgaatttcc gtaatggtta atggcttctc gcgtggtttc gccagcattg
     2641 ccgtggtagc cataatcagg ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg
     2701 cataagcctg ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga
     2761 tctcttttcc ttcagcccag gcggcaaggt ataagcaact gtctattgct gtgccgtcga
     2821 ccaacaccgt acaggcacca cattcaccca cgcagcaccc ttgtttgaca cttagcagtc
     2881 cttgttcgcg gagtaattcc gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca
     2941 tcccgttaat ggtgcattcg atggtaattg tttcgctgtg attcattgca attttccccc
     3001 cgccgcggcg acggcttcgc taatcacttt tttggtcatc gtctggatga gatgcagacg
     3061 aaactcttta ctggcccgcc atgaagaacg cggggcgaca tcttgcagga cagattcgct
     3121 gatagcttcc agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg
     3181 gcagcgaatc ggcgttggcg cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc
     3241 gttatccagt cggcaatgtg cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat
     3301 ggcatattta aaatgcgcgc tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc
     3361 gacgaggatt tcgtcatgct caagagacac tttgcccggc ccggtgtgaa agccattaat
     3421 cgggacgaaa cgaacaccgc gcggggagtg gatctccagt ttcgcgtcat aaattagcgt
     3481 tggcgtggca gaatctgcgc tggtggcacc gttgcaaata tttccaccgt aggtagcgac
     3541 gttacggatc tgcagtccag caatggacat ggccgcagca cataacgccg ggagatgacg
     3601 ttgagttata ggatcttcta ttagctgggt aaatgtcgtt gcagagccga tacgtagcga
     3661 gccatcttcc gccagcgtaa ttccccgcag ctccgccaga ttatgaatat caacaatatg
     3721 gcgataacgg tcattgtgat ggtggagctg aatcagtacg tcagtgccac cggcgagcag
     3781 tttggcctgc gggttgttag ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg
     3841 gtaagaagca aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga tggaactctt
     3901 catataaccg ttttggcgtc agcggcagtg tattgattgc aacaccggta gccatcttca
     3961 ccgcgttacg aatagcagcg gcaacaggaa ttattggtgg ctcacccagt gacttatgtc
     4021 cgtatgcgga ttgcggctca ttgatttcga cgaacgcgct ttccagttgt ggcagatccg
     4081 gcatggtcgg cattttgtaa tccagcagat tggggttacg gaccacgccg cttttagcat
     4141 cgatgatcat ctcttcaaat agcgcccagc caatgcccat tcccattccg ccgtgtacct
     4201 gaccttctgc cagcagtgga ttaagaatat gccctgaatc atgaacgttg aggatgcggt
     4261 tgatggtgac tttgcacagc gcaatatcga ccgtcagatc aacaaaggta cagccaaacg
     4321 ccggtgggtt agtggtggtt ttgatggagc tttcagcaga gagctgcccg ccgcgttcag
     4381 ggtggtagaa agcgtccatc gccaaatctt ttaacgacat taacggctct tccggtcgtt
     4441 caaccagcac gatatggcct tttatcaggg tcagattcat cgctgactga tgtagcatga
     4501 ctgcggcgtg agcgatgatt ttctctttta ataatagtgc cgcactgcgc agcgcaggcg
     4561 cggcaacata gctctggcgt gaggcaaatg tgccgggatc gaacggcgta acgtcggtat
     4621 cttgagttga aataacgcga acgtcgctga ccggaacccc cacggtttct gccaccattt
     4681 gcgagaagac ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc acgttgatgg
     4741 ttccatcctg attcatcaga aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt
     4801 tagaggtgta gctaaaacag gcgacgccaa cgccgcggcg caaattgcct tgctggttct
     4861 ggcattctgc acggcgtttt tcccattcaa agattttccg gcctttttca agacactccg
     4921 gcaaccctgc gctgtaaata cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg
     4981 cgttgcgtaa acgaatttca acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca
     5041 tagactcaac ggcaaatacg acttgtggcg cgccataacc acgcatcgca ccagccgagg
     5101 ggaggttggt atagcaggtc tttgaactgt aagcgtaggc acaacgagga taaaggtaag
     5161 cgactttatt cccccccgca gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt
     5221 tagacagaac atccagacta taacctttca atgttccgtc gcggttcacg cccatttgcc
     5281 cgtcaatggt aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca cggctaaggg
     5341 aaactttcac cggaatgccg ccaagcttgc tggtcaggaa tgccgccatt ggctcttcca
     5401 gtacatcctg tttattacca aaaccgccac cgacaaatgg tttgatgact cgtacgcatg
     5461 accagggaat atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc
     5521 tggaaacgat ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt acgctttcca
     5581 tgtgacaatg ttgaataacg ggggtctgat agtgcccctg tacctggtag tcggcggcat
     5641 cgattgtttg ttggacatta cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt
     5701 tatggattgg tgcagcgtct tctgccagcg ccgcttctgg cgtggtgata acgggtaatt
     5761 cttgccactc aatgctgacc aattgcgccg ctttttctgc cgtgagttca tcgcgggcca
     5821 cgacgatggc aatggcgtca ccatgatgac gaacatggcg agttagcagt gcgcgatcgg
     5881 cggtatcgcg cttgttttcg tcaagtgtcc aggcatgccc agctgtagcg aatggaatat
     5941 caggcacatc ttcccaggta aaaatcgcca gtacgcccgg caaacttctg gcttgttcat
     6001 cattaatact tacggcataa ccatgtgcga tagggctacg tacatatttc gcataacaca
     6061 tgcccgccat aacataatcg tcagtatatc gtgcccgccc ggtgacctta gcaatggcat
     6121 cgacgcgcat gcatgattca cccgtagcgg ttgcttcccg cgcttccata gaaatcccct
     6181 cgatttgaac atttggttct tatggcaaag atttagtcta agcaggcaag atttacgcca
     6241 gaaacgacga taagcgttag gggaggtagg aagtgtgatg acgttaaaaa aacacagata
     6301 tgatacgaaa caggagtctg aataggggca gtgatacgta tcaatatgag tggcgataaa
     6361 atcaatgtga taaattggga ttattacgca tcaggat
//
LOCUS       DADLTD010000209         6361 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLTD010000209 DADLTD010000000
VERSION     DADLTD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.211"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..260)
                     /locus_tag="JN701_003514"
     CDS             complement(<1..260)
                     /locus_tag="JN701_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365831.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAILRCTGNSGHYH"
     gene            complement(513..983)
                     /gene="bcp"
                     /locus_tag="JN701_003515"
     CDS             complement(513..983)
                     /gene="bcp"
                     /locus_tag="JN701_003515"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY7365832.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(983..1555)
                     /locus_tag="JN701_003516"
     CDS             complement(983..1555)
                     /locus_tag="JN701_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY7365833.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1701..2579
                     /gene="dapA"
                     /locus_tag="JN701_003517"
     CDS             1701..2579
                     /gene="dapA"
                     /locus_tag="JN701_003517"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY7365834.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SARETVRAALKHAGLL"
     gene            2596..3630
                     /gene="bamC"
                     /locus_tag="JN701_003518"
     CDS             2596..3630
                     /gene="bamC"
                     /locus_tag="JN701_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY7365835.1"
                     /translation="MAYSVQKSRLAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3843..4556
                     /gene="purC"
                     /locus_tag="JN701_003519"
     CDS             3843..4556
                     /gene="purC"
                     /locus_tag="JN701_003519"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY7365836.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4724..5587
                     /locus_tag="JN701_003520"
     CDS             4724..5587
                     /locus_tag="JN701_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY7365837.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5602..>6361
                     /locus_tag="JN701_003521"
     CDS             5602..>6361
                     /locus_tag="JN701_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7365838.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASD"
BASE COUNT         1542 a         1556 c         1755 g         1508 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaggatcg catcatccct ctgggagatg
       61 gcattgaccg ggcagacctg caggcaaggg gcttcctcac agtgatgaca cacgacaggg
      121 gcagtgattg ttgacgtctt cgccagggcc aggcgcgggt gttgctgtaa accttgcgtt
      181 ttatgcacgt ccgaacaggc agcgagacag gtattacatc ctgtacacca cagtggttcg
      241 gccaccacaa agcggttcat gtcggctcct tgaaaatgat atgaaacatt gcatttcata
      301 ttgcataaag gatgccagtc tgattaattt aatatattca ataaattatg tattaacagg
      361 gttctgaacg gtacaaaaat gatggtcatt aacgttcagc aaaaaatgga ttcgacaccc
      421 gtgtcgcagg ggaaagcgag aatgtgatct tgatcacgag aagtgaggtg tgctgaccgc
      481 aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt gttctttcag ccagttcagc
      541 acaacgtcgt ggtgattgct ggttttaaaa tcgtcaaaga catgttcgat tttgccatca
      601 gcgtcaatca ggaagctgat gcgatgaata ccatcgtagg ttttgcccat gaaggacttt
      661 tcaccccaga cgccgaattg ttcgcacacc tggtggtcct catcagacag gagcgtaaag
      721 ttaagcagct ctttttccgc aaaacgggag agtttttcgg gtttatcggt gctgataccc
      781 agcacatcaa cgcccgcttt tttcaactca tccatgttat cgcgtaagcc gcaggcctgt
      841 acggtacagc cgggggtcat ggctttcggg tagaaataaa ccagaacacg ctgtccctgg
      901 aagtcggtca aattaacttg ttctccgtct tgatccggca agctaaattt cggtgcgata
      961 tcaccggctt tcagtggatt cattacttaa ctccatcctg ttcatcatgt tgggaataat
     1021 tgacgacgtt aatactgcct tgtgcattga gttctgtaca tagggctttg aacgcttgct
     1081 caatatttgc tgcgtccgca gatgcggggc tgtgggcggt gatctgaata tgcaactgcg
     1141 cagcccgttc attttcagca ggttgcgtgc gcgacaccag ctccgcaatg ttcatatgat
     1201 gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg ggagtctgca acatcgacct
     1261 gaacccagac agatgctggc attggcggac gcggacgcgc cgtcgtgcgc ttcatcacga
     1321 ttaaaagatc cagttcggca cctttcaacg gtaacgttga ttcaatcaga gtaatggcat
     1381 tccatgaacc ggaaagcagc atgataaacg tgaactcttc tcccagcatc gccaggcgac
     1441 tgtcttcaat attacagccg caactactga catgacgggt gatggtgttc acaattccag
     1501 ggcgatcggc acccaacgca gtgatcacca gataatgttg cgatgacagt gtcaaactgg
     1561 ttattccttt aaggggtgag ttgttcttaa ggaaagcata aaaaaaacat gcatacaaca
     1621 atcagaacgg ttctgtctgc ttgcttttaa tgccatacca aacgtaccat tgagacactt
     1681 gtttgcacag aggatggccc atgttcacgg gaagtattgt cgcgattgtt actccgatgg
     1741 atgaaaaagg taatgtctgt cgggctagct tgaaaaaact gattgattat catgtcgcca
     1801 gcggtacttc ggcgatcgtt tctgttggca ccactggcga gtccgctacc ttaaatcatg
     1861 acgaacatgc tgatgtggtg atgatgacgc tggagctggc tgacgggcgc attccggtga
     1921 ttgccgggac cggtgctaac gctactgcgg aagccattag cctgacgcag cgcttcaatg
     1981 acagcggtat cgtcggctgc ctgacggtaa ccccttacta caatcgtccg tcgcaagaag
     2041 gtttgtatca gcatttcaaa gccatcgctg aacatactga cctgccgcaa attctgtata
     2101 atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac ggtgggccgt ctggcgaaag
     2161 taaaaaatat tatcggaatc aaagaggcaa cagggaactt aacgcgtgta aaccagatca
     2221 aagagctggt ttcagatgat tttgttctgc tgagcggcga tgatgcgagc gcgctggact
     2281 tcatgcaatt aggcggtcat ggggttattt ccgttacggc taacgtcgca gcgcgtgata
     2341 tggcccagat gtgcaaactg gcagcagaag ggcattttgc cgaggcacgc gttattaatc
     2401 agcgtctgat gccattacac aacaaactat ttgtcgaacc caatccaatc ccggtgaaat
     2461 gggcatgtaa ggaactgggt cttgtggcga ccgatacgct gcgcctgcca atgacaccaa
     2521 tcaccgacag tgctcgtgag acggtcagag cggcgcttaa gcatgccggt ttgctgtaaa
     2581 gtttagggag atttgatggc ttactctgtt caaaagtcgc gcctggcaaa ggttgcgggt
     2641 gtttcgctta ttttattact cgctgcctgt agttctgact cacgctataa gcgtcaggtc
     2701 agtggtgatg aagcctacct ggaagcggca ccgcttgcgg agcttcatgc cccggctgga
     2761 atgattttgc cggtgacctc cggtgattat gcaatcccgg tgaccaacgg tagtggtgct
     2821 gtcggtaagg cgctggacat tcgtccacca gcccagccgc tggcactggt ttctggcgtg
     2881 cgtacccagt tcacgggcga taccgcttca ttgctggtgg aaaatggtcg tggcaatact
     2941 ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact acaccatcac ccaacgtgat
     3001 gatgctggtc agacactgac catcgattgg gtacaatgga accgtctgga cgaagacgag
     3061 cagtatcgtg gtcgttatca aatctctgtt aagccgcagg gttatcagca ggcggttacg
     3121 gttaaactgc tgaacctgga acaggcgggc aaaccggttg cagacgcggc ttccatgcag
     3181 cgttacagca cggagatgat gaacgttatt tccgccggtc tggataaatc tgcctctgac
     3241 gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg acgtacaaag tgcagctgat
     3301 gacaccggtt taccgatgct ggtcgtacgc gggccgttca atgtggtttg gcaacgtctg
     3361 ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca gcacccgttc gcagggtaac
     3421 atggccgtaa cttataagcc gctgtctgac agcgactggc aggaactggg cgcgagcgat
     3481 ccaggcctgg catccggtga ctataaactg caggttggcg atttagataa ccgcagcagc
     3541 ctacagttca tcgatccgaa aggtcatact ctgactcaga gtcagaacga cgcgctggta
     3601 gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg ctggagtcat ccggcccttt
     3661 tttctgatat gatacgcaaa cgtgtgcgtc ggcagaaaaa tgcaatttta gcggtaattc
     3721 gcacgaaatt tgtttgtcgg acgtaggtcg gataaggcgt tcttgccgca tccgacaaaa
     3781 catccggcac accagacagc aaaagatttt aaaacgttaa ttcacaccca ggagtgataa
     3841 agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa aaccgtatac agcacggaaa
     3901 acccggacct gttggtgctc gaattccgca atgatacgtc agcaggggat ggcgcgcgca
     3961 ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt caactacttc attatgagca
     4021 aactggctga agcgggtatc ccgactcaaa tggagcgtct gctctccgat accgaatgtc
     4081 tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt gcgtaaccgt gctgctggct
     4141 ctctggtgaa acgtcttgga atcgaagaag gtattgagct gaacccgccg ctgttcgatc
     4201 tgttcctgaa aaacgatgcc atgcacgatc cgatggtcaa cgaatcttac tgcgaaacct
     4261 ttggctgggt gagcaaagag aacctggcgc gtatgaaaga gctgacctac aaagcgaacg
     4321 acgtgctgaa aaaacggttc gatgatgctg gtctgattct ggtcgacttc aagctggagt
     4381 ttggtctgta caaaggtgaa gtggtactgg gcgatgagtt ctccccggac ggcagccgcc
     4441 tgtgggacaa agaaacactg gagaaaatgg acaaagaccg tttccgccag agcctcggtg
     4501 gcctgatcga agcctatgaa gccgtcgccc gccgcctggg tgtacagctg gactgatttt
     4561 tctgttcatc atcttgccgt gcagctggca cggcaagaca accgctctcg gaatatatgc
     4621 attgaacgct cattcctccg ccatttcatc ccgctttcct gtggtaatct tcctctgaac
     4681 cacctacgat catttctata atgaatatat tgttgaggta tctatgcgtt ggcaagggcg
     4741 acgtgaaagt gacaatgttg aagacaggcg caacagctcc ggtggtccgt ctatgggcgg
     4801 tcccggtttt cgcctgccaa gcggtaaagg cgggctgatt ttactgatag tcgtgctggt
     4861 tgcaggctac tatggtgttg atttaaccgg gttaatgact ggacagccgg tttcccaaca
     4921 acaatcaacg cggtccatta gccctaatga agacgaagct gcaaaattca cctcggtgat
     4981 tctggcaacc acggaagaca cctggggaca acagttcgag aagatgggta agacctatca
     5041 gcaaccgaag ctggtcatgt accgtggaat gacgcgcacc ggctgcgggg cgggccagtc
     5101 cattatgggg ccgttctatt gcccggcgga tggcacagtt tatatcgatc tctccttcta
     5161 tgatgacatg aaagacaaac ttggcgcgga tggcgatttt gcccaggggt acgttatcgc
     5221 ccatgaagtc ggtcatcatg tgcagaaact gttaggcatc gagccgaaag ttcgtcaact
     5281 gcaacaaaac gcgacgcagg cggaagtgaa ccgcttatct gtgcgtatgg aactccaggc
     5341 cgactgtttt gccggtgtct gggggcataa catgcagcag caaggcgttc tggaaaccgg
     5401 cgatctggaa gaggcgctga atgcggcgca ggccatcggc gatgaccgtt tacaacagca
     5461 aagtcagggg cgagtagtac cagacagttt cacacatggc acttctcagc aacgctacag
     5521 ctggtttaaa cgtggtttcg acagcggcga tccggcacaa tgcaatactt ttggtaaaag
     5581 catttaattt ccggggcagg gatggctgaa ctgactgcgc tttacacatt aacagcgcaa
     5641 atgaaacgtg aagggatccg ccgcttgctg gtgttgagcg gggaagaggg ttggtgtttt
     5701 gatcatgcgc ttaagttgcg tgatgcctta cttggcgact ggctgtggat ttcgccgcag
     5761 ccagatgctg aaaaccactg ttctccctcg gcgctacaaa ctttacttgg gcgcgagttc
     5821 cggcatgcgg tattcgacgc ccgccacggc tttgatgccg ctgcctttgc ggcacttagc
     5881 ggaacgttga aagcgggaag ctggctggtt ttgttactcc ctgtatggga agagtgggaa
     5941 aaccaacctg atgccgactc gctgcgctgg agtgattgcc ctgaccctat tgcgacgccg
     6001 cattttgtcc agcatctcaa acgcgtactt acggcggata acgacgctat tctctggcgg
     6061 caaaaccagc cgttctcgtt ggcgcatttt actccccgta ctgactggca ccccgcgacc
     6121 ggcgcaccac aaccagaaca acagcaactc ttacagcagc tactgaccat gccgccgggc
     6181 gtggcagcgg taacggctgc gcgtgggcgc ggtaagtcgg cgctggcagg gcaactcatt
     6241 tctcgtattg cgggcagtgc gattgtcacc gcgcccgcaa aagcggcaac ggatgtactg
     6301 gcacaatttg cgggcgagaa gtttcgcttt attgcgccgg atgccttgtt agccagcgat
     6361 g
//
LOCUS       DADLTD010000210         6337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLTD010000210 DADLTD010000000
VERSION     DADLTD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.212"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            30..152
                     /locus_tag="JN701_003522"
     CDS             30..152
                     /locus_tag="JN701_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7365839.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            168..1013
                     /locus_tag="JN701_003523"
     CDS             168..1013
                     /locus_tag="JN701_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7365840.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1036..2547
                     /locus_tag="JN701_003524"
     CDS             1036..2547
                     /locus_tag="JN701_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7365841.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGGRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2572..3570
                     /locus_tag="JN701_003525"
     CDS             2572..3570
                     /locus_tag="JN701_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7365842.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3603..4697
                     /locus_tag="JN701_003526"
     CDS             3603..4697
                     /locus_tag="JN701_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7365843.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4709..5581
                     /locus_tag="JN701_003527"
     CDS             4709..5581
                     /locus_tag="JN701_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7365844.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5629..6051)
                     /locus_tag="JN701_003528"
     CDS             complement(5629..6051)
                     /locus_tag="JN701_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7365845.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6148..>6337)
                     /locus_tag="JN701_003529"
     CDS             complement(6148..>6337)
                     /locus_tag="JN701_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HAY7365846.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT         1467 a         1514 c         1836 g         1520 t
ORIGIN      
        1 ttgttaataa aaaccctaca ggaggcacta tgcctaaaaa aatgagaaca acccgtaatc
       61 tattattaat ggctacgcta ttaggtagcg cactatttgc cagggctgcg gataaagaaa
      121 tgaccattgg tgcaatatac cttgatacct agggatatta cgctggagtg cgtcagggcg
      181 ttcaggatgc ggcaaaagat tcttcagtac aggtacagtt aattgaaact aacgcccagg
      241 gtgatatttc gaaagaaagt acctttgttg ataccctcgt ggcgcgtaat gtcgatgcca
      301 ttattttatc ggcagtgtct gaaaatggca gtagccgtac cgttcgtcgc gccagtgaag
      361 cgggtattcc ggtgatttgc tacaacacct gtattaatca aaagggtgtc gataaatatg
      421 tctcggcgta tctggtcggc gatccactgg aatttggtaa aaaactgagt aacgctgccg
      481 ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc aattgcgaag
      541 cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga agtattaaaa tcccgcgttc
      601 ccggcgcgca aattgtcgct aatcaggaag ggactgtttt agataaagcg atttccgttg
      661 gtgaaaaact gattatctcc acgccggatc tcaacgccat tatgggggag tcgggcggtg
      721 cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa attgctgttt
      781 tcggttcgga tatgacaacc gaaattgctc aggagctgga aaacaatcag gtgctgaaag
      841 cggtagtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa acattgaagg
      901 ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat ctctatacca
      961 aaacggaaga tggtaaacag tggctggcaa cgcacgttga tggtctgccc taatcgctga
     1021 ttcgcagagg tccctatgtt cacggcaaca gaggcagtcc cggtagcaaa agtggtggca
     1081 ggaaataagc gttaccccgg cgtcgttgcg ctggataacg ttaacttcac gctcaataaa
     1141 ggcgaagttc gtgcgctgtt aggtaaaaac ggcgcgggca aatcgactct cattcgaatg
     1201 cttaccggca gcgaacgccc ggatagcggt gatatctgga ttggcgagac gcgactggaa
     1261 ggtgacgaag ctacgttgac tcgccgtgcc gctgaactgg ggggtcgtgc ggtttatcag
     1321 gaattaagtc tggtggaagg gctgacggtg gcagaaaacc tctgccttgg tcaatggcct
     1381 cgccgcaacg gcatgattga ttacctgcaa atggcgcagg atgcccaacg ttgcttacag
     1441 gcgctgggcg ttgacgttag tcctgaacaa cttgtttcaa cgctaagccc ggcgcaaaag
     1501 caactggtgg aaattgcacg ggtgatgaag ggcgagccgc gcgtggtcat tcttgatgaa
     1561 cccaccagct cgcttgccag tgcagaagtt gaactggtga tcagtgcggt gaaaaagatg
     1621 tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat ccgccgcatt
     1681 gcctcctgtg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat gctcgaaaac
     1741 acctccacgc atcatattgt gtcgctaatg ctagggcgtg atcacgttga tattgcgccg
     1801 gttgcccctc aggaaattgt ggatcaggcc gtactggaag tccgtgcgtt acgccataag
     1861 cccaagctgg aggatatcag ctttacgtta cgtcgcggcg aagtgcttgg cattgctggc
     1921 ctgctggggg cagggcgcag tgaattgctg aaggcgattg ttgggctgga gacgtatgaa
     1981 cagggcgaaa ttgttatcaa cggcgagaaa atcacgcgcc ccgattacgg cgacatgctg
     2041 aaacgcggca ttggctatac gccagaaaac cgcaaagaag cggggatcat tccctggttg
     2101 ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcgccaa cggagtgctg
     2161 caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac ggtcaaggcc
     2221 gccagtagcg aaacgcccat cggcacgctt tctggtggca atcagcaaaa agtggtgatc
     2281 ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac gcgcggcgtc
     2341 gatatcgaag ccaaacagca gatttaccgt attgtccgtg agctggctgc cgaaggaaaa
     2401 agcgtggtgt ttatctccag tgaagtggag gagttgccgc tggtgtgcga ccgcatcctg
     2461 ttgttacagc acggtacgtt ctcgcaggag tttcactcgc cggtcaatgt ggatgagctg
     2521 atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa gatgtctgct
     2581 tcgtcattat cattgccgct gggcaagagc gtctcgctca aacaatttgt cagtcgccat
     2641 attaatgaga tcggtttgct ggtggtaatc gccattcttt atctggtctt ctccctgaat
     2701 gcgcctggct ttatctcatt gaataaccag atgaacgtgc ttcgcgatgc tgccaccatc
     2761 gggatcgccg cctgggcaat gacgctgatt attatctccg gtgaaattga tgtcagcgtt
     2821 ggaccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt tgaagttccg
     2881 ttggcgattg cttgtctgct ggtgttgctg ttaggcgcgc tgatgggaac gctcgccggg
     2941 gtgctgcgcg gcgtgtttaa cgtgcccagt ttcgttgcca cactggggct gtggagcgcc
     3001 ctgcgcggaa tggggctgtt tatgaccaac gcactgccag tgccgattga cgaaaacgag
     3061 gtgctggact ggctgggcgg acaatttctc ggtgtgccag tatccgcgct gatcatgatg
     3121 gtgttgtttg cgctgtttgt gttcattagc cgaaaaaccg ccttcgggcg ctcggttttt
     3181 gccgttggcg gtaatgccac ggcggcgcag ttgtgcggta ttaacgttcg tcgagtgcgc
     3241 attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt gctggcggca
     3301 cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt catcgccgcg
     3361 gtcgtggtcg gcggtacggc actttccggc ggtcgcggtt ccctgttcgg tacattgctt
     3421 ggtgtgctgg tgattacgct aatcggtaac ggtctggtgc tgctcggtat taactccttt
     3481 ttccagcagg tggtacgcgg cgtcatcatc gtggtggcgg tgctggcgaa tatcttgctg
     3541 acccagcgaa gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg ggtgcggcag
     3601 ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg gcagcttatt
     3661 taccaggaaa ttcaaccgtt gatctgcggg aagttgcggt gccgacgccg gggattaacc
     3721 aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac tatatctatc
     3781 atcaacaccg tgccacggcg gcggcacccg ataaaccgtt ataccaggga tttatcaacg
     3841 gtcatgaacc atgcgggcag attgtggcga tggggcaagg ctgtcgccat tttaaagagg
     3901 gtgatcgcgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac tgccgccgcg
     3961 gctttcctat ctcttgtact ggcaaaggaa aagcggctta cggctggcag cgtgacggcg
     4021 gtcatgccga atacttgctg gcggaagaaa aagatctgat cctcctgccg gatgcgctta
     4081 gctacgaaga tggtgcgttt atcagttgcg gcgttggtac agcgtatgaa ggaattttgc
     4141 gcggcgaagt ttccggcagt gataacgtgc tggtggtcgg tctggggcca gtcggcatga
     4201 tggcgatgat gctggcgaaa ggtcgcggtg caaaacggat catcggcgtt gatatgctgc
     4261 cggaacgtct ggcgatggca aaacagttag gggtgatgga tcacggctat ttagcaacca
     4321 ccgaaggtct gccgcagatt atcgccgaac tcacccacgg tggcgcggat gttgcgctcg
     4381 attgttctgg taatgccgca ggtcgcttgc tggcactgcg atccaccgct gattggggac
     4441 gggtggttta catcggagaa accggaaaag tggagttcga ggtcagcgcc gatctgatgc
     4501 accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg gaaaaatgcg
     4561 cccatgatct gacggactgg aaactgtggc cgcgtaacgc cattacccat cgcttctcgc
     4621 tggaacaggc tggagatgcc tatgcgctga tggcgagcgg caaatgcggg aaagttgtga
     4681 ttaacttccc ggattaagga gttagcggat gaccatctat accttatctc acggaccgct
     4741 aaagctggat gtctctgacc agggcggcgt aatcgaaggc ttctggcgtg acacgacgcc
     4801 gcttttacgt cctggtaaaa aaagcggtgt ggcaaccgat gcttcctgtt ttccgctggt
     4861 gccgtttgca aaccgggtga gcggcaatcg ttttgtctgg caggggcgtg agtatcaact
     4921 gcaaccgaat gttgagtggg atgcgcacta tctacacggc gatggctggc tgggcgagtg
     4981 gcaatgtgtc tcgcacagcg atgatagctt gtgtctggtg tatgaacatc gcagcggtgt
     5041 ctatcactat cgggtaagtc aggcgtttca tttaacggcg gatacgctga cggtgacgct
     5101 ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc atccttattt
     5161 tccgttgtca ccacaaacgc ggattcaggc gcaggcgagc ggttactggc tggagcggga
     5221 gcagtggctg gcgggtgagt tttgcgagca gctaccgcag gaactggatt ttaaccagct
     5281 cgcgccgttg ccgcgccagt gggtgaacaa tggttttgcc ggatggaatg gtcaggcccg
     5341 catcgagcag ccgcaggaag gttatgccat catcatggaa acgacgccac ctgcaccgtg
     5401 ttatttcatc tttgtttccg accctgcgtt tgataaagga tatgcgtttg atttcttctg
     5461 tctggaaccg atgagccatg cgccggatga tcatcatcgc ccggaagggg gtgatctcat
     5521 tgcgctagca ccaggggaat caacaacttc agagatgtcg ttgcgggtag agtggctgta
     5581 atttattaaa tgccggataa ggcgttcacg ccgcatccgg catctttcct aacgccgatc
     5641 gagagaaatt gcccccggcc cggtaattgc caatagcaag aacgcgccag cgatactgac
     5701 attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat cccagtaatg
     5761 gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca gcggacgggt
     5821 gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg caataatcgc
     5881 tgccagcatc ggcattggcg cgcccaacga ggccatatat tggaccgtac cgtcaaagcc
     5941 catcattttg ggaaaaccca aaataatgaa aattaagacc acggcgatac gggcaattaa
     6001 taacaaaacg gggcgggcag ctccaaaatc aaaataacgt agtgtgttca taaccaatcc
     6061 ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc acgccccagg
     6121 cgttgagata ttcgctaatt atcctggtta cagtgattta agcgcgatgt tctcatctat
     6181 cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcgaattg ggcgaatctc
     6241 acgcccctga ttcagcgtga ccgcaccgat aagcacgccg ttttgcagat taaaccaaat
     6301 cgccttctga gtttccgggt tgccacgaca aagccag
//
LOCUS       DADLTD010000211         6329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLTD010000211 DADLTD010000000
VERSION     DADLTD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.213"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..150
                     /locus_tag="JN701_003530"
     CDS             <1..150
                     /locus_tag="JN701_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7365847.1"
                     /translation="AIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            239..1699
                     /locus_tag="JN701_003531"
     CDS             239..1699
                     /locus_tag="JN701_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7365848.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYKVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1735..1938)
                     /gene="ydfZ"
                     /locus_tag="JN701_003532"
     CDS             complement(1735..1938)
                     /gene="ydfZ"
                     /locus_tag="JN701_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY7365849.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1935..2801)
                     /locus_tag="JN701_003533"
     CDS             complement(1935..2801)
                     /locus_tag="JN701_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7365850.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIEHKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVARSA
                     RKTATGLAKSNSFTLIPSGMRGFSPRLTAHACIKKDVSLIKNKKYFTRQEYLYCARYV
                     GFDCKSERRKHL"
     gene            complement(2890..3636)
                     /gene="ydfG"
                     /locus_tag="JN701_003534"
     CDS             complement(2890..3636)
                     /gene="ydfG"
                     /locus_tag="JN701_003534"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY7365851.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3773..5818
                     /gene="dcp"
                     /locus_tag="JN701_003535"
     CDS             3773..5818
                     /gene="dcp"
                     /locus_tag="JN701_003535"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY7365852.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5863..>6329)
                     /locus_tag="JN701_003536"
     CDS             complement(5863..>6329)
                     /locus_tag="JN701_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY7365853.1"
                     /translation="NLTSALSQRLIADQLRLTTAESCTGGKLASALCAAEDTPKFYGA
                     GFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYGGLE
                     GGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1585 a         1510 c         1627 g         1607 t
ORIGIN      
        1 gccattatga tcatggcata ttcagtgatt ggtttaatct ctgcgctgaa aatgccagag
       61 gtgaaagacc gtgatttaag tgcgctggaa gacgctgcgg aagatcaacc gcgtgttgta
      121 agagctgcgc aaccttccag aagtctgtaa accctcaatc ccttctctta ccggagaggg
      181 gatttttatt catataacaa aacatatagc ttgccatatt taaatttaag gaattgttat
      241 gggaaataat ttgttatcag caaaagcgac actccctgtt tatgatcgta ataacctggc
      301 cccaagaatt gttcatttag gctttggtgc atttcaccgt gcgcatcagg gcgtgtatgc
      361 cgatattctt gctacagaac atttcagtga ctggggatat tataaggtca acttaatcgg
      421 cggcgaacag caaattgccg atttacaaca gcaagataat ctttataccg ttgcggaaat
      481 gtcggccgat gcgtggacgg ctcgcgtcgt tggcgtcgtt aaaaaagcct tgcacgtaca
      541 gatggatggc ttagaaaccg tgttggctgc gatgtgtgaa ccgcaaatcg cgattgtctc
      601 tctgacaatc accgaaaaag ggtatttcca ctctccggcg accggacagt taatgctcga
      661 tcacccgatg gtcgctgccg acgtgcaaaa tccccaccag ccgaaaacag caacaggggt
      721 gattgttgag gcgctggccc gccgtaaagc ggcaggactt cccgcattta ccgtcatgtc
      781 atgtgacaac atgccagaaa acggtcatgt tatgcgtgac gttgtcactt catacgcgaa
      841 agctgttgat gtaaaactgg cacaatggat cgaagataac gtgactttcc catcaacaat
      901 ggtggaccgt attgtgcccg cagtgacaga ggatacgctg gcgaaaatcg aacaacttac
      961 cggtgtgcgc gatcctgcgg gcgttgcctg tgaacctttc cgccagtggg taatagaaga
     1021 taactttgtt gccggacgtc cagaatggga aaaagcggga gccgaactgg ttagtgatgt
     1081 gctgccttat gaagagatga agttgcgcat gctcaacggc agtcattcat tcctggcgta
     1141 tttgggttat cttgccggat atcagcacat taatgactgc atggaagatg aacattatcg
     1201 ttatgcggcg tatggcttga tgttgcagga acaagcgccg acgctgaaag tgcagggcgt
     1261 tgatttgcaa gattacgcta accgattaat tgaacgctat agcaatccgg cgttacgtca
     1321 tcgaacctgg cagattgcga tggatggtag ccagaaattg ccacagcgga tgttggattc
     1381 tgttcgctgg catctggcgc atgacagcaa gttcgatctg ctggcgctgg gcgtcgcggg
     1441 ttggatgcgt tatgtcggtg gtgttgatga acagggaaat ccgatagaaa tcagtgaccc
     1501 actgttacct gttattcaga aggctgtaca aagtagtgcc gaagggaaag cgcgcgtcca
     1561 gtcattgctg gcgattaagg caatctttgg tgatgatttg ccagacaata gcttgtttac
     1621 tgcaaaagtg acggaagcgt acttgtcttt attagcgcat ggcgcgaaag cgaccgtggc
     1681 gaaatattcc gtgaagtaaa aaaagcaatg ccgccatcgg cggcattcac acacttagtt
     1741 catgccgaga cgaatcaggt ccagaggtgc cagtttctct tcacaacctt caacaactac
     1801 ggtttttccg cggcggattt gctcagcggt cagaccttca gtatcaatcg acaggatctt
     1861 gccagtgtga ccggtgccgc taaccataac acggctgcca gtggtgattg cgttacggtt
     1921 acgatcgtag gtcgtcatag gtgttttctc ctttctgatt tacagtcgaa gcccacgtaa
     1981 cgggcgcaat ataaatactc ctgtcgagtg aaatattttt tgtttttgat caagctcaca
     2041 tcttttttga tgcatgcatg agccgtaagg cggggggaaa agcctctcat cccggatggg
     2101 atgagagtaa atgaattact cttcgctaaa ccagtcgctg ttttcctggc ggatctggcc
     2161 acggattcgc tgatttcctg caaatgttgc gtcattgcac gttctaccgc atcgccatca
     2221 cgtttttgca gggcagagaa aatatcaaga tgctggcgta acagcatttc tggtggagaa
     2281 acatggtcga aactcatata gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta
     2341 tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt
     2401 tcaaaaaaat catccagttg cttgtgctca atggcaatgc gttgctggtg aagattttgt
     2461 tccagttggt agcactggct ttcggtaatc atgctcgccg cccgacgcgc caccgcgcac
     2521 tcaatggcct gacggataaa actgccgttg cgcacctggg ccatggaaat tttgttgacg
     2581 tagctgccac gctgtggacg aatttgaatc aggccgtttt ccgccagttt aataaaggct
     2641 tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa cttctttttc cgacaacggt
     2701 gtgcctggag caatcaggca atggacaatg tcgcgacgaa ggatacgata aatttgctga
     2761 ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca ttcgttcttg cttgtgagtg
     2821 agttaactgc atgagtctac cacttttttg tgacaagcaa taacccggca gttacgccgg
     2881 gtataaaaat tactgacggt ggacattcag tccggcatag ctttgggtaa ccggcatcat
     2941 ttccagggta ttgatattga cgtgagcagg cagcgttgac acccaccaaa cggcttcgct
     3001 gacatcttct ggcgtcaatg caacggtatt ttgataggtt ttttccgctt taccgtcatc
     3061 gcctttaaag cggacattgg aaaactcggt gccacccacc agacccggtt cgatgtcggt
     3121 gacgcgcacc gccgtaccat gcaggtccgt acgcagattc aggctaaact gacgaacaaa
     3181 cgctttcgtc gcaccgtaaa cgttaccacc ggcataaggc cagctacctg ccgttgagcc
     3241 aatgttaata atatgaccat gattacgttc aaccataccc ggtaagacgg cgcgcgtcat
     3301 atataccagg cctttgttgt tggtatcaat catcgtttcc cagtcttcaa cgctggcttt
     3361 atgcgcaggc tccatgccca acgccaggcc ggcattattt accaggatat caatattgca
     3421 ccactcggca ggaagcgatg ccagcatctc ttcaatagcg gcgcggttgc gaacgtccag
     3481 ttgggcgatg tacagattat ctcccagttc gtcttttaac tcctgcaacc gttcctggcg
     3541 acggccagtg gcgataactt tatgcccttg ttgaataaaa cgacgagtaa tgcattcacc
     3601 aaaacctgcc gttgctccag ttactaaaac gatcatctca ctgttcctca acgcttttgt
     3661 gtgtcaataa catagcacgt gatgcttaag agcgggtaat actggtttgt aagattgcac
     3721 tccgttatca gtaagcctac gctatggaac acctcgcgtt caggagaaga aaatgacaac
     3781 aatgaatcct ttccttgtgc aaagcacact accgtatctg gctccccatt ttgatcaaat
     3841 tgccaatcat cactatcgcc cggcattcga tgagggaatg cagcaaaagc gggcagaaat
     3901 tgctgccatc gcgcttaacc cgcaaacgcc tgatttcaac aatactattc tggcactgga
     3961 acaaagcgga gaattactta cccgcgttac cagcgtcttt tttgcgatga ctgcggcaca
     4021 taccaatgat gaattacagc gtcttgatga acagttttcc gctgaactgg cggaactggc
     4081 taatgatatc tatctgaacg gtgaattatt cgcgcgggta gatgctgtct ggcagcgacg
     4141 tgaatccctg gggcttgata gtgaatccat ccgcctggtg gaggtgattc atcaacgttt
     4201 tgtccttgcc ggagccaaac ttgcgcaagc tgataaagca aaattaaaag tactgaatac
     4261 agaagctgcg accctgacca gccagtttaa ccagcgatta ctggcagcaa ataaatccgg
     4321 cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg agtgagcaag agattgcgct
     4381 ggcggcagag gcggctcgcg agaaaggtct ggataacaaa tggctgattc cgctgctgaa
     4441 taccacccaa caaccggcgc ttgccgagat gcgcgatcgt gcgacgcgtg aaaaactgtt
     4501 tattgcgggc tggacgcgag cggaaaaaaa tgatgccaat gatacccgcg ctatcattca
     4561 acgtctggta gagattcgcg cacagcaggc gaaactgctt ggttttcctc attatgccgc
     4621 atggaaaatc gccgatcaga tggcaaaaac gccagaagca gcactcaact ttatgcggga
     4681 aattgttcca gcggcgcgtc aacgtgctag cgatgaatta gcctccatac aggcggttat
     4741 cgataagcag caaggcgggt ttagcgcgca gccgtgggac tgggcatttt atgccgaaca
     4801 ggtacggcgg gagaaatttg atcttgatga ggcgcagctc aagccatatt ttgaattaaa
     4861 cacggtgttg aatgaaggtg tattctggac cgcgaatcag ctcttcggta ttaagtttgt
     4921 cgaacgtttt gatattcctg tctaccatcc tgacgttcgt gtgtgggaaa tttttgatca
     4981 taatggcgtg ggactggcgt tattttacgg tgatttcttc gcccgtgatt caaaaagcgg
     5041 cggtgcatgg atgggcaatt ttgttgagca atcaacgctt aatgaaacgc atccggtaat
     5101 ttataacgtt tgcaattatc agaaacccgc tgccggtgag cctgcgttgt tactctggga
     5161 tgatgtcata accttattcc atgaatttgg tcatacgctg cacggccttt ttgcccgcca
     5221 gcgttatgcc acgctttccg gcaccaacac gccgcgtgat tttgtcgaat ttccgtcgca
     5281 aatcaacgaa cactgggcaa cgcatccgca ggtattcgct cgctacgctc ggcattatca
     5341 gagcggggca gcaatgcctg acgaactgca acagaaaatg cgtaatgcca gcctgttcaa
     5401 caaagggtat gagatgagcg aactgcttag cgccgcactt ctcgatatgc gctggcattg
     5461 cctggaagaa aacgaagcaa tgcaggatgt cgatgatttt gaattgcggg cgctggtggc
     5521 ggaaaatatg gatcttcctg ctataccgcc acgctatcgc agcagttatt tcgcccatat
     5581 ttttggtggc ggatatgctg caggttatta cgcttatctg tggacgcaaa tgttggccga
     5641 tgatgggtac cagtggtttg ttgagcaggg cggattaacg cgtgaaaatg ggcagcgttt
     5701 tcgcgaggcg atcctttcca gaggtaacag cgaagatctg gaacgcctgt atcgacaatg
     5761 gcgcggtaag gctcctcaga ttatgccgat gctgcaacat cgtggcttga atatataaac
     5821 accaccaatg gctggcctgg agatatcgcc aggccagcct ggttatagca gtaattgcag
     5881 cagctggacg tgggcaaacc tcaccgctaa agctaatacc gtttcgcagt cgccagcaaa
     5941 atgcataacc gcagtgtagg tctggccttt aatatgccac gcgaaccaga cggtacccgc
     6001 tggcgtacca tcttcaccgc cctccagtcc gccgtagccg gtaatggcaa tactgacatc
     6061 agcatccgca cgctctatgg cacctgttgc catttctgct gccactttct cactcaccgc
     6121 agaatatcgt tcaagagatt gctggcttac gctgaggatt ttcatctttg cctgatcggt
     6181 gaaagtaaca aagcctgcac cgtaaaattt gggtgtatct tcagctgcac acagggcgct
     6241 agccaactta ccgccggtgc atgattcggc ggtagttaag cgtaattgat ccgcgataag
     6301 tctttgactc aacgcggatg tcaggtttt
//
LOCUS       DADLTD010000212         6317 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLTD010000212 DADLTD010000000
VERSION     DADLTD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.214"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            285..626
                     /gene="raiA"
                     /locus_tag="JN701_003537"
     CDS             285..626
                     /gene="raiA"
                     /locus_tag="JN701_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY7365854.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            730..777
                     /locus_tag="JN701_003538"
     CDS             730..777
                     /locus_tag="JN701_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365855.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            877..2037
                     /gene="pheA"
                     /locus_tag="JN701_003539"
     CDS             877..2037
                     /gene="pheA"
                     /locus_tag="JN701_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY7365856.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHCEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2080..3201)
                     /gene="tyrA"
                     /locus_tag="JN701_003540"
     CDS             complement(2080..3201)
                     /gene="tyrA"
                     /locus_tag="JN701_003540"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY7365857.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3212..4282)
                     /gene="aroF"
                     /locus_tag="JN701_003541"
     CDS             complement(3212..4282)
                     /gene="aroF"
                     /locus_tag="JN701_003541"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY7365858.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4490..4855
                     /locus_tag="JN701_003542"
     CDS             4490..4855
                     /locus_tag="JN701_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY7365859.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            5005..5523
                     /locus_tag="JN701_003543"
     CDS             5005..5523
                     /locus_tag="JN701_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HAY7365860.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5513..>6317
                     /gene="dgcN"
                     /locus_tag="JN701_003544"
     CDS             5513..>6317
                     /gene="dgcN"
                     /locus_tag="JN701_003544"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HAY7365861.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGIN"
BASE COUNT         1718 a         1612 c         1420 g         1567 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattacag cccttgagct gccgtttttt
       61 tattctgtta gttgtgaaac tgaagcgatt tagtcactat cgatctcatc aaatatggct
      121 cgctttgaga tattcctcaa gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga
      181 ttcgttgaca aaaagtgaca aaattatgag attcccatca cacattttga catcaggaac
      241 ggtatgctga attcaccaag acgggaagac aagaggtaaa atttatgaca atgaacatta
      301 ccagcaaaca aatggaaatt actccggcca tccgccaaca tgtcgcagac cgtctcgcca
      361 aactggaaaa atggcaaaca catctgatta atccacatat cattctgtcc aaagagccac
      421 aagggtttgt tgctgacgcc acaatcaata cacctaacgg cgttctggtt gccagtggta
      481 aacatgaaga tatgtacact gcaattaacg aattgatcaa caagctggaa cggcagctca
      541 ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact
      601 tcgtcgaaga agttgaagaa gagtagtcct ttatattgag tgtatcgcca acgcgccttc
      661 gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta ctgtactaaa gtcacttaag
      721 gaaacaaaca tgaaacacac accgtttttc ttcgcattct tttttacctt cccctgaatg
      781 ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac aataaggcct cccaaaccgg
      841 ggggcctttt ttattgataa caaaaaaggc aacactatga catcggaaaa tccattactg
      901 gcgctgcgag agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcgg
      961 cgcgaactgg ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgagat
     1021 attgatcgtg aacgcgattt actggaaaga ttaattacgc tcggtaaagc gcaccatctg
     1081 gacgcccatt acattactcg cctgttccag ctcatcattg aagattccgt attaactcag
     1141 caggctttgc tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt
     1201 ctcggcccca aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcactgt
     1261 gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc
     1321 ggccaggccg actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaacgac
     1381 gtttacgatc tgctgcaaca taccagcttg tcgattgttg gcgagatgac gttaactatc
     1441 gaccattgtt tgttggtctc cggcacgact gatttatcca ccattaatac ggtctacagc
     1501 catccgcagc cattccagca atgcagcaaa ttccttaatc gttatcctca ctggaagatt
     1561 gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat
     1621 gttgctgcgt tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt
     1681 attgaagcga atcagcaaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt
     1741 aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa tggcgaccgg gcaacaagcc
     1801 ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca atctgattat gacccgtctg
     1861 gaatcacgcc cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc
     1921 aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt taggtgaaat tacccgttca
     1981 atgaaggtat tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga
     2041 aaaggtgccg gatgatgtga atcatccggc actggattat tactggcggt tgtcattcgc
     2101 ctgacgcaat aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg
     2161 ctccaccttg cggaaactgt caataaacgc ctgcttatcg ccctgcttca gcaactcaat
     2221 cgcctcgccg aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat
     2281 aataatgtcg gcataaagct gcggatcctg agcaaacagt cgcccgacca tcgccagctc
     2341 aaggcggtaa atcggcgaag agagcgccag aagttgctca agctgaacat tttcttctgc
     2401 cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga gcctgaataa acgccatatt
     2461 ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc
     2521 cagaaaccat tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgctttgc
     2581 caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc agtaccgggc catcgtgcgc
     2641 cgccagcatg gcctgtaatg gtccattttt cactgatgcc agatcaacca gaatacaatc
     2701 tttcggtaaa ggcggtaatt tgccgataac ttgctcagta acgtggattg gcacactaac
     2761 aatcaccatt ccggcatcgg caacaatatc agccgctcga tcccagtcat gttgctccag
     2821 aatccgcacc tgataacccg agagtgtcag catcttctcg aacaggcgtc ccatctgacc
     2881 gccaccgccg acgataacca ccggacgcag tgacggacaa agtgttttaa atcctttgtc
     2941 gttttcactg gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg
     3001 cggtaccccc agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc
     3061 cggaacataa ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc
     3121 cagacgcttc gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa
     3181 tgcggtcaat tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt
     3241 tcagatcctg atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat
     3301 cggttacgga tacaccgtat ttcatttcac tgcgcggttg ctcagaagac tgattgccct
     3361 cgtggatatt actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag
     3421 caaccacgga ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc
     3481 tgcaatctac catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg
     3541 caacatccgc agggctatag ttcggcgctt taccaccgcg caggatgaca tgcccgtccg
     3601 gattcccctg agtttgtagc aacgcaacct gccctgcctg gttaatgcca acaaaacggt
     3661 gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc cagactgccg tcggtgccgt
     3721 ttttaaaacc aaccggcatg gaaagcccgg aggccatttc acggtgagtt tgcgattccg
     3781 ttgtacgagc accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg
     3841 gatctaacgc ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac
     3901 gcgcgatctg cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatta
     3961 accctttcca gccgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga
     4021 ggctatcgct gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt
     4081 ccagatcatg aatggaacaa ggaccacaaa ctaccagcag acgaggatcg cgcccggcga
     4141 taatatctga aatggtttta cgcgagtcag caatctgggc ttcttgttgc aggctcaatg
     4201 gaaaagcggc cttcagttgt tccggagtca ttaaaacctg ttcgtcggta atatgtacgt
     4261 tattcagcgc gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat
     4321 cctcagcgag gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta
     4381 cgcttttctt tacacgaaca atttactcgc gctttgatgc ttatttcgta caaataaatt
     4441 tacaccccct ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa
     4501 gtttatcgcc cccttattgg ctttactggt tagcggatgt cagattgatc cttatactca
     4561 cgcgccaacc ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg
     4621 cagcgccata aaagatgacg atgcatttgg cgattcacag gcggatcgcg gtctatacct
     4681 taaaggatat gccgaaggac aaaagaaaac ttgccagacc gattttactt atgcccgggg
     4741 actttccggt aaaagctttc ctgccagctg taacaatgtt gaaagtgcca gccaactgca
     4801 tgaagtatgg caaaaagggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa
     4861 accccacaaa taaattaaac ataagatttt actcattgtg ttttatttct cacattgatg
     4921 acgatcgcat gaaaaaatga tattttaatg agcattctga aaaaatatcg atgcatttcg
     4981 agcgaagaag gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt
     5041 ctcctgctga cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg
     5101 cgtatgatgg tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca
     5161 aagttatgta tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca
     5221 tctttgcctt atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc
     5281 aatggttttt attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat
     5341 ccgtcaaagg cgttattatt aattgccaaa caaaataccg agtgcattat tggtagcgcc
     5401 ttttgtctga tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg
     5461 cgtagcggtg taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga
     5521 taacgataat tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat
     5581 gaccagcata tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct
     5641 gaccctgaaa cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag
     5701 tctggaagca gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct
     5761 gggccagcaa gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc
     5821 gtcctggcat tacacccgta aggatccagg cgatactttc agcaatttca taagccactg
     5881 gctcttcccc gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt
     5941 acgcttaacc gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact
     6001 gaccggttgt attctgctgg catcaggcat cgcaattacc ctcacccgcc atttgcacaa
     6061 tggcctagtg gaagcgctga aaaatatcac cgatgtcgta catgatgtgc gttccaaccg
     6121 caatttttcc cgacgagttt ctgaagaacg tatcgctgag tttcaccgct tcgctctcga
     6181 cttcaacagt ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc
     6241 gcagcttctg cgtaccgcgc tacatgaccc attaaccggg ctggctaacc gcgcagcgtt
     6301 tcgtagcggc atcaata
//
LOCUS       DADLTD010000213         6293 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLTD010000213 DADLTD010000000
VERSION     DADLTD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.215"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(85..621)
                     /gene="ssb1"
                     /locus_tag="JN701_003545"
     CDS             complement(85..621)
                     /gene="ssb1"
                     /locus_tag="JN701_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY7365862.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            875..3697
                     /gene="uvrA"
                     /locus_tag="JN701_003546"
     CDS             875..3697
                     /gene="uvrA"
                     /locus_tag="JN701_003546"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY7365863.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3732..4088)
                     /locus_tag="JN701_003547"
     CDS             complement(3732..4088)
                     /locus_tag="JN701_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7365864.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4092..4508)
                     /locus_tag="JN701_003548"
     CDS             complement(4092..4508)
                     /locus_tag="JN701_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7365865.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4619..5332)
                     /gene="aphA"
                     /locus_tag="JN701_003549"
                     /pseudo
     CDS             complement(4619..5332)
                     /gene="aphA"
                     /locus_tag="JN701_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
     gene            5695..6237
                     /locus_tag="JN701_003550"
     CDS             5695..6237
                     /locus_tag="JN701_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365866.1"
                     /translation="MVINFITRDGDDDMNISYMNSNKTTSLPVELDALNNKDISYAKD
                     FSYVKDFFLYIETQLKIAKDFCRLGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
BASE COUNT         1594 a         1553 c         1567 g         1579 t
ORIGIN      
        1 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
       61 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
      121 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
      181 ctgattgcca ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc
      241 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
      301 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
      361 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
      421 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
      481 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
      541 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
      601 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
      661 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
      721 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
      781 ttcattcagg ttaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
      841 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
      901 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
      961 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     1021 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     1081 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     1141 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     1201 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     1261 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     1321 gctactcgcg ccaatcatta aagaacgcaa aggcgaacac accaaaacgc tggagaacct
     1381 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     1441 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     1501 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     1561 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     1621 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccac gactgttttc
     1681 gtttaacaac ccggcaggtg cctgcccaac ctgtgacggc cttggcgtac agcaatattt
     1741 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     1801 ctgggatcgc cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa
     1861 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     1921 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     1981 ccgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacaga
     2041 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     2101 cgaagggacg cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc
     2161 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactagc
     2221 tggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     2281 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     2341 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     2401 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     2461 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     2521 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     2581 tgaagtggtc gcagaaggtc cgctggaagc gatcatggcg gtgcctgaat cgttgaccgg
     2641 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     2701 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     2761 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     2821 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     2881 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     2941 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     3001 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     3061 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     3121 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     3181 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     3241 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     3301 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     3361 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     3421 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     3481 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     3541 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     3601 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     3661 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     3721 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     3781 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     3841 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     3901 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     3961 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     4021 tcgttatgca cgctctgttc tgcgcctagt tttgccatgc aatattgtag caactccgaa
     4081 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     4141 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     4201 ggcaatacaa gcgatgttcc ctgcattgag gatttgatat gagaaggcat atcgtctgct
     4261 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     4321 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     4381 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     4441 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     4501 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     4561 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     4621 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     4681 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     4741 taatatcatt atcagaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgct
     4801 attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag
     4861 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc ggactacgac
     4921 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     4981 cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat
     5041 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aagcccgggc
     5101 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     5161 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     5221 caacgttagt accagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     5281 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     5341 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     5401 ataacgccag ctgcagggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     5461 tgattatatt taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta
     5521 ctcgatgtaa ggatacatta attttacgtt tattatgatg aatcctctgc aaatgagcga
     5581 aatttgatag aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa
     5641 aaacaatcga agtttataaa gatgattcct gattgaccag aaccctatcc tacaatggtg
     5701 attaatttta ttactcgcga tggagatgat gatatgaata tatcatatat gaatagcaac
     5761 aaaacgacat ctttacctgt tgagctagat gcactaaata ataaagatat ctcttatgcc
     5821 aaagattttt cttatgtcaa agatttcttt ttatacatag aaacacagtt aaaaattgca
     5881 aaagattttt gtagacttgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac
     5941 gcatttattg atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg
     6001 ctttgctgtt ttgccgagga ggttaaaggt gattattctc attacaggac ctttttattt
     6061 gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga gttctcttta
     6121 acattagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag
     6181 catattcttg aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt
     6241 taataatggt tagatgtgca gaaattatta ctccacacga aaaaataggg aaa
//
LOCUS       DADLTD010000214         6281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.216, whole genome shotgun sequence.
ACCESSION   DADLTD010000214 DADLTD010000000
VERSION     DADLTD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.216"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(2..94)
                     /locus_tag="JN701_003551"
     tRNA            complement(2..94)
                     /locus_tag="JN701_003551"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN701_003552"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN701_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY7365867.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN701_003553"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN701_003553"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY7365868.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN701_003554"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN701_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY7365869.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN701_003555"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN701_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY7365870.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN701_003556"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN701_003556"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY7365871.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..>6281)
                     /locus_tag="JN701_003557"
     CDS             complement(6114..>6281)
                     /locus_tag="JN701_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="membrane-bound lytic murein transglycosylase B;
                     catalyzes the cleavage of the glycosidic bonds between
                     N-acetylmuramic acid and N-acetylglucosamine in
                     peptidoglycan; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase B"
                     /protein_id="HAY7365872.1"
                     /translation="GNHQQASLLRLDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQ
                     LGQAVALARVQ"
BASE COUNT         1468 a         1762 c         1500 g         1551 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt cacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgt
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggtgtttgat
     1981 ctagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgctgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactgattca tcccggcgtt ggtaaacaac aaagttggat
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcat catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccatgcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggacgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 caccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg
     5041 agcacagcag gttgtcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtatg
     5461 ttcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcgcctatca ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttca
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgcaaa tagttactgc
     6121 acgcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttataac gggtaatggt gtagaagttc ggcagaccgt accagtactg gtaaccggta
     6241 ccgacatcca gacgcagcag gctggcttgt tgatggttgc c
//
LOCUS       DADLTD010000215         6256 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.217, whole genome shotgun sequence.
ACCESSION   DADLTD010000215 DADLTD010000000
VERSION     DADLTD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.217"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..174)
                     /locus_tag="JN701_003558"
     CDS             complement(<1..174)
                     /locus_tag="JN701_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HAY7365873.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKG"
     gene            complement(571..810)
                     /locus_tag="JN701_003559"
     CDS             complement(571..810)
                     /locus_tag="JN701_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY7365874.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(813..1532)
                     /gene="sanA"
                     /locus_tag="JN701_003560"
     CDS             complement(813..1532)
                     /gene="sanA"
                     /locus_tag="JN701_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY7365875.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1682..2566)
                     /gene="cdd"
                     /locus_tag="JN701_003561"
     CDS             complement(1682..2566)
                     /gene="cdd"
                     /locus_tag="JN701_003561"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7365876.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2696..3391)
                     /locus_tag="JN701_003562"
     CDS             complement(2696..3391)
                     /locus_tag="JN701_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY7365877.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(3388..3786)
                     /locus_tag="JN701_003563"
     CDS             complement(3388..3786)
                     /locus_tag="JN701_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY7365878.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            4025..4975
                     /gene="dusC"
                     /locus_tag="JN701_003564"
                     /pseudo
     CDS             4025..4975
                     /gene="dusC"
                     /locus_tag="JN701_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311941.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(5363..5446)
                     /gene="yohP"
                     /locus_tag="JN701_003565"
     CDS             complement(5363..5446)
                     /gene="yohP"
                     /locus_tag="JN701_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY7365879.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            5580..>6256
                     /locus_tag="JN701_003566"
     CDS             5580..>6256
                     /locus_tag="JN701_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAY7365880.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEW"
BASE COUNT         1652 a         1581 c         1581 g         1442 t
ORIGIN      
        1 gcctttaaaa ttacgaaagt agattgagcg aataaatttc cccggatcgg tctgtgccgg
       61 gcaagcctga ctacagggag cgtcgtgaca taataaacag cgagaggctt ctttaatcgc
      121 cagtaacggc gtaaaagccg gagtgagttc atccagataa ttttgttgcg gcatgtgatc
      181 ttccttaatg ctatgagtaa ttaaggcgaa cgctaaatat actcgtcata cttcaagttg
      241 catgtgctgc gtctgcgggt tattcggccc atccatgggc ctcacccctt atggggccgc
      301 tgcaggcagc gttcaaatcc gctccatgcg gatttgtcgc tcaccccagt cacttactta
      361 tgtaagctcc tggggactca ctcacttgtc gccttcctgc aactcgaatt atttagagta
      421 taaatattgc gtgctgatat taaggctatg cgcgtgggga aatttatact gtgaaaggat
      481 tcacaaatag ctgcggttca gaatgttata tccgcagctt attgagtgaa gtaataatta
      541 ttctcaataa ttaacagaaa agagaaaata ttaacgatag aaaataagat aaacaatcat
      601 gccgatagat aaaattccga agccgatata tgccaccata taagcgaaaa agagtccggg
      661 attttttgct cgccagacat acaccggttc cggtgcattt ttaacccgtt tatcaatcgc
      721 gcctgcgcgc cagcctttcc atgcttcgcg gaaacaaaat atcggaataa ggaaaaaaac
      781 ggcaacgaca aaaaactgct gtacatccat aactactttc cttgtttctt ttgtaattca
      841 agtaactgtt cgggtgtgac ggcgggatac ccctgcgcat cttccggcac ctggtgcata
      901 gccggaatag ggaccagcgg ccctaaaaaa cgcggttcac gtttaaaaat ataaaggtca
      961 gccagcgcac cgaaacgggc ggcaaattcg cgaatacgta ctgacagcat atctttcggt
     1021 gacggtacgg cataacactg agcctgaatc cccatatgca gcgcaataaa taatgcccgc
     1081 tcacagtgga aacgttgggt gataataatg aaatcattag tatcgaaaac tttgcgtgta
     1141 cgcacgatgg aatccagcgt acgaaagcct gcgtaatcga gaacaatatc tgatgggtcg
     1201 acaccagcag cgattaaatc tttgcgcatg gtcatcggct cattataact ttgcaatgcg
     1261 ttatcgccgc tcagtaatag ataatttacc ttaccgctgt tataggcatt aatcgctcct
     1321 tgaatgcggt agcgataata ctgattaatt acgccagtac gataatattt tgctgttccg
     1381 agcaccacac cgacctggcg gtaggggaga tcctgcaatt cgtcgtagat ataaggcgcg
     1441 gttttccagc tcatccagcg atcgaggccg agcacagtca acaacagcaa gccgatcagg
     1501 actaacaggc tgaggaacac gcgctttaac atgcaggaga ctcgatctaa ggaaaatttc
     1561 catcaggcta ctttacccgc tggttttgcg caagaaacgg caaactgttt gccgggaatt
     1621 tcgacaccag gagagggctg gcaagccgga caaggcgttc acgccgcatc cggcaccagg
     1681 cttaagcgag aagcactcgg tcgatactgt gacagccgag agctttcagc gttgcggagg
     1741 tggcatccca ctgaatcaac ggcgcatcgg ctttttctgc cagaaccgcg cgctggatat
     1801 ccgggtaatc ataacccttg agattcaaca gaattaacgc tccctgcaac ggtggcagag
     1861 tcgggttgaa tgcggcgttt tcagcgtagc tgccactgaa aatacgaccg tctttacatt
     1921 ccagcgcgac accgcttggc gacttactgt aaggcatgtg cgaacggttt gccgctgcaa
     1981 tcgccgcctg agaaagcgca tcaccagtca gcgcatagcc gtgatcctgc tcgtccatca
     2041 gcagcgtttt aatctccaga tctttcggcc caaaggcatc cggcagatag tcacgcagcg
     2101 cgtgtgcctc gcggcccggc agatgaatac gcagatccag accgctgttc agttcattca
     2161 taaactgacg gcagtgacca caaggcgtgt agttaacggt gatggctgca agcgcttttt
     2221 caccactcaa ccaggcgtgg ctgatcgcgc tttgttcggc atgaacggtt tgctgcattg
     2281 tcgcaccaat aaattccata ttggcaccga aataccaggt tccgctcaca ccgcgcgcaa
     2341 ttgcgccaac attaaaattc gacaatggcg tacgcgcaca ggccgctgcc agcggaagta
     2401 gtgcgaatgc cagcgcgtct tcgtccagcc ccgttgcgct cttcagcgat gagacttgct
     2461 ccccggtcaa caaagcgggg aagtacttgt ctgccagaat aggttccagt gcagattgca
     2521 agttatccgc aagttgggca aaagcggttt gaaaacgtgg atgcatggcg ttgcctcata
     2581 atcatgtaat gggataacag tttacgaacc cgttatggct ttatatgtga tctgaatctc
     2641 attaattatg caacgcgtga aacttacatc aaaaatgcgc gacgcatcgc aaattttagc
     2701 ccattactgc cagaataatc gggaaaagga acggtgcgat cagcgaagta attatcccgc
     2761 ataacaccag cgctagcgaa ctaaatgcac cttcctgata atccagctcg gcgcagcgcg
     2821 ccgtaccgag ggcgtgcgag gcagtcccca ttgccagacc gcgcgcagct ttggtacgaa
     2881 tacgcatcgc attaagcaat gtatggccaa ataccgcgcc gaggatgccg acgaaaatca
     2941 cgcaaactgc gctgattgcc ggaataccgc caatactgcc gccaaccgcc attgcaatag
     3001 gcgtggtgac tgatttcggc aggattgacg cggcgatttc cggtgaagcg cccatcaaca
     3061 atgccacgga agtccccgtc accatcgcaa ccacgctgcc gataaaacag atggtgatga
     3121 tcgatttcca gcgcgcgcgg atctggtgta gctgctcata taaaggatag gccagcgcga
     3181 cgaccgccgg ttgcagcaga tcgtttagca cttcgctacc tttaaagtag ctgtcgtaag
     3241 agatgccggt cagcattaaa aaagggatga tcaccaccat tgctaccagc aacgggttaa
     3301 gcaagggaaa cttataccgt gccgccagtt tgcgggcggc aaaaaagacg atcaatgtca
     3361 gtggtaatga ccaccagata ttagccatca ttcttctgat cctttctgac ctacgacttt
     3421 acgttcaccg tgcaccagtt gcgaactcca gctcaccacc agaaaaacca ccagtgtact
     3481 gacagcacag gaaaccacta ccgggccaaa ctgtgcgcgg agcaaatcaa aatattgcat
     3541 gacgcctacg ccaatcggca caaacaatag cgccatatag cgaatcagta cgtagcaccc
     3601 cggattgacc cattttgccg gaagaatttg caaggccagc aggacaaaca ggatcagcat
     3661 cccgatgatg ctgcccggaa tggttaccgg tagcagggaa gcaatgaaaa tgcctgcata
     3721 caggcaggca taaatcagga cgaaagcgcg taaatattgc cagataatgt tcagtgtctt
     3781 actcataatc aacaccccag atgagatgaa ttcatcatac aattaattcg taaaacgtgc
     3841 taccgatcac attaagaaat cagcgcttat tttttcgctc cccggcatta cgcaatgcgc
     3901 aacgaactca ggcatacatc aagcatagag agggattaca gtttgcagca atccctcttt
     3961 gcagaccttt aatccgttga ccataggcgc taacatagcg cctcattttt ttgcgggtga
     4021 agatatgcgt gtgttactgg ctccgatgga gggtgtgctt gactctctgg tgcgtgaatt
     4081 gctgaccgaa gttaacgact acgatctgtg catcaccgag tttgtccgcg tggtggatca
     4141 actgctgccg gtaaaagttt ttcatcgcat ttgccctgag ctacaaaacg ccagccggac
     4201 accatccggt acgctggtgc gcgtgcagtt gttgggtcag ttcccacaat ggctggcaga
     4261 gaatgccgcc cgtgcggtgg aattaggttc ctggggcgtg gatctcaatt gcggctgccc
     4321 gtcgaaaacg gtcaacggta gcggtggtgg ggcgacgtta ctcaaagatc ctgaactcat
     4381 ctactagggt gcaaaagcga tgcgtgaagc tgtaccggcg catttgcccg tcagcgtgaa
     4441 agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa atcgccgatg cggttcaaca
     4501 ggctggtgct acggagctgg tggtgcatgg acggacaaaa gagcagggtt accgcgcgga
     4561 gcatattgac tggcaggcga ttggcgagat tcgccagcgg ctgaatattc cggtgattgc
     4621 caacggtgaa atctgggact ggcagagtgc gcaacaatgt atggcgatca gcggctgcga
     4681 cgcagtgatg attggtcgcg gggcgctcaa tattcccaac ctgagccggg tggtaaaata
     4741 taacgaaccg cgaatgccgt ggccggaggt ggttgctttg ctgcaaaaat atacccgtct
     4801 ggaaaagcag ggcgataccg ggttatatca cgttgcgcgg attaaacagt ggttgagtta
     4861 tttgcgtaaa gaatacgatg aagcaacgga attatttcag catgttcggg tgttgaataa
     4921 ttcccctgat attgcaaggg ctattcaggc aattgatatc gataagctgc gataatagtg
     4981 gaatcgcagt ttatcagaga caatgaatcg cttattcagc ttcatcatct tgttgcgctc
     5041 ctttcatgag ctaagccaca tattgccact ggcgcaagga gcgcgcaggg ggaggacaat
     5101 cgcctcctcc ccctgccccc ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt
     5161 cagcttatcc ccttcggcta ccgggtcggg cgcgaggtaa cgtccttgta agacgcgccc
     5221 tcagcccaca tccatgtggg ctgccccggc cttcggggaa cgcttcagcg attttgacgc
     5281 caccaacacc ggagctgttt gcaaatcaag tacacaaaat atctagttcc tgacctgaca
     5341 acttatatgc catatctgat ttttaaaata tcatcttaaa tacgccagtc accaccagta
     5401 gcccaatcag gaaaataatc aatacagccc agagtataat tttcattggc gttcctttat
     5461 ttgtgagtgt tgaataagta taggaaaaaa cgaagccctt tgccgttatc cataaacgat
     5521 taagataaat cggaagatat cattcactta gtctatattt ttctgggtat gtcgttttat
     5581 tgctactgat gtattgccac gcgaaactaa aaaatatatc gcaacacacg gtaatctccg
     5641 cgcaccgttt cttacctgat tattccccca tgaatcgtga ttccttttat ccagccatcg
     5701 cctgttttcc gctgttattg atgctggccg ggtgtgcgcc tatgcatgaa acccgccagg
     5761 cgttaagcca gcaaacgccc gctgcacaag ttgacaccgc attacccacg gcgctgaaaa
     5821 atggttggcc agacagccaa tggtggctgg agtatcacga taatcagctc acttcattaa
     5881 ttaacaatgc actacagaac gcaccggata tgcaggtggc tgaacagcgc attcagctag
     5941 ctgaagccca ggcgaaggca gttgccactc aggatggtcc gcagatcgac ttttcggcgg
     6001 atatggagcg gcaaaaaatg tcggcagaag gcttaatggg gccgtttgct ctgaacgatc
     6061 cggccgcagg tacgaccggc ccgtggtaca ccaacggtac ttttggctta acggcgggct
     6121 ggcatctcga tatctgggga aagaatcggg cggaggttac tgcccgcctg ggtacggtta
     6181 aagcacgggc ggcggaacgc gagcaaaccc gccaattgct ggctggcagc gtagcccgcc
     6241 tgtactggga gtggca
//
LOCUS       DADLTD010000216         6248 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLTD010000216 DADLTD010000000
VERSION     DADLTD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.218"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..3975
                     /locus_tag="JN701_003567"
                     /pseudo
     CDS             1..3975
                     /locus_tag="JN701_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            3976..5625
                     /locus_tag="JN701_003568"
     CDS             3976..5625
                     /locus_tag="JN701_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY7365881.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            5630..>6248
                     /locus_tag="JN701_003569"
     CDS             5630..>6248
                     /locus_tag="JN701_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365882.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         1487 a         1584 c         1798 g         1379 t
ORIGIN      
        1 ctggaaggcg catccagcaa tttcatctcg ccgcagccgg gtaacattta tattcctctc
       61 ggccaacaag agccgggact gtacctcgtc gaggcgatgg ttggtgggta tcgggcgacg
      121 acggtggtgt ttgtttccga taccgtggct cttagcaaag tgtcaggcaa cgagcttctg
      181 gtgtggaccg cgggtaaaaa acagggtgaa gcgaagcccg gctcagagat cttgtggact
      241 gacggtcttg gcgtgatgac ccgcggtgtg accgatgaca gcggtacctt gcagttacaa
      301 catatatcgc cagaacgttc atacattctg ggtaaggatg ctgaaggcgg cgtttttgtc
      361 tccgagaact tcttctacga aagcgaaatc tacaacaccc gcttgtatat ttttaccgat
      421 cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag tgatgggccg cgagttccac
      481 gatccgttgc attcatcccc catcgtcagc gccccggcga agctttcggt gctggacgct
      541 aacggcagtc tgttgcaaac cgtcgatgtc acgctggatg cgcgcaatgg cgggcaggga
      601 agtttccgcc tgccagaaaa tgccgttgcc ggaggttatg agttacgtct tgcttaccgc
      661 aatcaggtct atagcagcag ttttcgcgtg gcaaactaca tcaagccaca tttcgagatt
      721 ggtttagctc tcgacaaaaa agagttcaaa actggcgaag cggtcagcgg caaactgcaa
      781 ctgctctacc cggatggcga gccggtaaaa aatgcccgcg tgcagttaag tttgcgcgct
      841 cagcaattat caatggtcgg taacgatttg cgttatgccg gacgtttccc cgtgtcgctg
      901 gaaggcagcg aaacggtgtc cgacgccagc ggtcatgtgg cgttaaatct ccccgccgcc
      961 gataaaccga gccgttattt gttaaccgtc tccgccagtg acggcgcggc gtatcgtgtc
     1021 accaccacca aagagatcct cattgaacgc ggtctggcgc attactcatt aagtactgcc
     1081 gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt atgccgcgct ggaatcttca
     1141 aaacaggttc ctgttacgta tgaatggttg cgtctcgaag accgcacgag ccatagcgga
     1201 gagctaccgt caggcggcaa atcctttacc gtcaatttcg ctaaacctgg caactacaat
     1261 ctgacattac gcgataaaga cggcttaatt ctcgctgggt taagttatgc cgtcagcggt
     1321 aagggcagca cggcgcatac gggtacggta gatatcgtgg cggataaaac gctgtaccag
     1381 ccaggcgaaa ccgcgaagat gctgattacc tttccggagc caattgatga agcattattg
     1441 acgctggaac gcgatcgcgt ggaacagcag tcgctgcttt cgcatccggc aaactggcta
     1501 acgctacaac gtttaaacga tacccagtat gaagcccggg ttccagtgag caattccttt
     1561 gcgcctaaca tcactttttc ggtgctgtat acccgtaatg gtcagtacag ttttcagaac
     1621 gccgggatca aagttgccgt tcctcagctt gatatccggg tgaaaacgga caaaacccat
     1681 taccagccag gtgaactggt caatgtcgaa ttaacctcgt cgctgaaagg taaacctgtt
     1741 tctgcgcagc taacggtagg cgtggtcgat gaaatgatct acgcgctgca accagaaatc
     1801 gcgccgaata tcggcaaatt tttctatccg ctggggcgta acaatgtgcg taccagctcc
     1861 agtctgtcgt ttatcagcta cgaccaggcg ctctccagcg agccggttgc gcctggcgca
     1921 actaaccgca gcgagcggcg agtaaaaatg cttgaacgtc cacggcgtga agaggtggat
     1981 accgcggcat ggatgccgtc actcacaacc gataaacaag gcaaagcgta tttcacgttc
     2041 ctgatgcctg attcgttaac ccgctggcgt atcaccgcgc gtgggatgaa cggcgacggg
     2101 ctggtcgggc aggggcgtgc ttatctgcgt tcggaaaaaa atctctacat gaagtggagt
     2161 atgccaacgg tgtatcgcgt gggcgacaaa ccggcggcag gactgtttat cttcagtcag
     2221 caggataacg aaccggtggc gctggtgact aaatttgcag gcgctgagat gcgccagacg
     2281 ctgacgctgc acaaaggggc gaattatatt tcgctggcgc agaatattca gcaatctggc
     2341 ttgttaagtg cagaactgca acaaaatggg caagtgcagg acagcattag cacaaaactg
     2401 tcttttgtgg acaacagcag gcccgttgaa cagcagaaaa atgtcatgct cggcggtggc
     2461 gagaacgcgc tgatgttgcc cgagcaggcg agtaatatcc ggctacaaag tagtgaaacg
     2521 ccgcaggaga ttttccgcaa caatcttgat gcgttagtcg atgaaccgtg gggtggggtg
     2581 atcaacaccg gtagccgtct gatcccgctc agtctcgcct ggcgttcgct tgccgatcat
     2641 caaagtgccg ccgctaacga cattcgtcag atgattcagg ataaccgtct gcggctgatg
     2701 caactggcgg ggcccggagc gcgctttacc tggtggggtg aagatggcaa tggtgacgcc
     2761 ttccttacgg catgggcatg gtacgccgac tggcaggcca gctaggcgct cggcgtaacg
     2821 caacaaccgg aatactggca gcatatgctc gacagttatg ccgagcaggc ggataacatg
     2881 ccgttattgc atcgggcgct ggtgctggcg tgggcacagg agatgaatct gccgtgcaaa
     2941 acgttgttga aagggttgga tgaagctatc gcccggcgcg gaactaaaga tgaagatttc
     3001 tctgaggaag acatccgcga tatcaatgac agcctgatcc tcgatacacc ggaatctcca
     3061 ctggcagatg cggtggcaaa cgtcttaacc atgacgttgc tgaaaaaagc gcagttgaag
     3121 tccacggtga tgccacaggt tcagcaatat gcgtgggata aagcggcaaa cagcaatcag
     3181 ccgctggcgc acacggttgt gctgctcaat agcggtggtg acgctaccca gacggccgct
     3241 attttaagtg gtttgaccgc tgagcaatcc actattgagc gcgcgctggc catgaactgg
     3301 ctggcgaaat atatggcgac aatgcctcca gttgttttgc ctgcgcctgc gggcgcatgg
     3361 gctaaacata agttaactgg agggggcgaa tactggcgtt gggttggtca gggcgtgccg
     3421 gacattctct cttttggtga cgaattatcg ccgcaaaatg tgcaggtccg ctggcgtgag
     3481 ccggcaaaaa tggctcaaca aagtaacatt ccggtgaccg ttgaacgcca gttgtatcgg
     3541 cttatccctg gtgaagaaga gatgagcttt attctgcaac cggtgaccag caatgagatt
     3601 gacagcgatg cgctgtatct cgatgacatc acgcttacca gcgagcagga tgcagttctg
     3661 cgctacggtc aggtggaagt accgctgcca ccgggagccg acgttgagcg cacaacatgg
     3721 ggcatttcgg tcaataaacc caacgccgcg aaacagcagg ggcaattgct ggaaaaagcg
     3781 cgaaatgaaa tgggcgaact ggcctatatg gtgccggtga aagaactgac gggaacggtc
     3841 actttccgcc atttgctgcg cttctcgcaa aaagggcaat tcgttctgcc tcctgctcgt
     3901 tatgtgcgtt cctatgcacc tgcacagcaa agtgttgcgg cagggagtga atggaccggg
     3961 atgcaggtga aataagtgaa ctggcgcaga atcgtctggc tattggcgtt ggttactcag
     4021 ccaacgcttg cggaggagac gccgttacaa ctggcactgc gtggtgcgca gcacgatcaa
     4081 ctgtatcagc tgtcgtcatc aggcgtgacc acagtctcag cattacctga ctcgctcacc
     4141 acgccgttag ggagtttgtg gaaactgtat gtttacgcct ggctggagga tacccatcaa
     4201 ccggagcagc cctaccagtg tcgcggaaac tccccggaag aggtttattg ttgtcaggct
     4261 ggggagagca taacgcgcga taccgcgctg gtacgttcct gtgggctcta ttttgcgccg
     4321 cagcggttgc atattggcgc agatgtgtgg gggcaatact ggcaacagcg tcaggcgcct
     4381 gcgtggttag cgtctcttac tacgctgaag ccagaaacct cggtaacggt taaatcgctg
     4441 cttgattcat tagcgacgtt acctgcgcaa aacaaagctc aggaagtctt actggatgtg
     4501 gtgctggacg aggcgaaaat cggtgtcgca tccatgctcg gtagtcgggt acgggtgaaa
     4561 acctggagct ggtttgccga tgataaacag gaaatccgtc agggtggttt tgccggctgg
     4621 ttaaccgatg gtacaccgct gtgggttact ggcagtggca ccagcaaaac ggtactaacc
     4681 cgttatgcga cagtgcttaa tcgggttttg ccagtgccga cgcaggttgc cagtgggcaa
     4741 tgtgtagagg tggagctgtt tgcccgctat ccgctgaaga aaatcacagc ggaaaagagt
     4801 acgacggcag taaaacccgg cgtacttaat gggcgctatc gtgtgacgtt tactaatggt
     4861 aaccacatta cgtttgtcag ccacggtgag acgaccttac tgagcgaaaa gggcaaactg
     4921 aaattgcagt cgcatctcga tcgtgaagag tacgttgcca gagttctgga tcgcgaggcg
     4981 aaaagtacgc cccctgaggc agcgaaagcc atgaccgtgg cgattcacac ttttttacag
     5041 caaaacgcca atcgggaagg cgattgcctg accatccccg acagcagcgc cacgcagcgt
     5101 gtttctgctt cgccagcgac cacgggggcg cgaacgatga ccgcctggac gcaggatctc
     5161 atctatgcag gcgatccggt tcattatcat ggcagtcgag ctaccgaagg gacactttcc
     5221 tggcggcaag ccacggccca ggcggggcag ggagagcgtt acgaccagat actcgcgttt
     5281 gcttatcccg acaacagcct tagccgctgg ggagcgccac gctcaacctg ccagttatta
     5341 cccaaagcga aagcctggct ggcgaaaaaa atgccgcagt ggcggcgtat attacaaggc
     5401 gagacggggt acaacgaacc agacgtgttt gcggtctgtc gtttagtctc aggtttcccc
     5461 tataccgatc ggcagcagaa acggctgttt attcgtaact tctttacgct tcaggatcgg
     5521 ctggatttaa cccatgagta tctgcacctg gcctttgatg gttatcccac cgggcttgat
     5581 gagaactata tcgaaacgct gacccgacaa ttattaatgg actaatgcta tgcgaaagat
     5641 ttttcttccg ttgttactgg tggcactttc gcctgtcgct cacagtgaag gtgtgcagga
     5701 agtcgagatt gacgcaccgc tctctggctg gcatccggca gagggcgaag atgccagttt
     5761 ttcacaatcg atcaattacc cggcgtcgtc cgtcaacatg gcagatgatc aaaatatctc
     5821 agcgcagatc cgcggcaaaa taaagaatta tgctgcggcg ggtaaaattc agcagggccg
     5881 gctggtggtc aacggtgcca gtatgccgca gagagttgaa tccgatggtt catttgcacg
     5941 cccttatatt ttcactgaag gcagcaacag cgtgcaggtc atcagcccgg atgggcaaag
     6001 ccgacaaaaa atgcagtttt actcaacgcc gggtacagga acgattcgtg cacgtttacg
     6061 gctggttctc tcgtgggata cggacaatac cgatctcgat ctccatgttg tcacgcccga
     6121 tggcgaacac gcctggtacg gtaacaccgt gctgaaaaac agtggtgcac tggatatgga
     6181 tgtcacgacg gggtacggac ccgagatttt cgccatgcca gcgccagttc acggacgcta
     6241 tcaggtgt
//
LOCUS       DADLTD010000217         6051 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLTD010000217 DADLTD010000000
VERSION     DADLTD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.219"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(95..2197)
                     /locus_tag="JN701_003570"
     CDS             complement(95..2197)
                     /locus_tag="JN701_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7365883.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2439..3500
                     /locus_tag="JN701_003571"
     CDS             2439..3500
                     /locus_tag="JN701_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAY7365884.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3613..5112)
                     /gene="ansP"
                     /locus_tag="JN701_003572"
     CDS             complement(3613..5112)
                     /gene="ansP"
                     /locus_tag="JN701_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HAY7365885.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5379..5996
                     /locus_tag="JN701_003573"
     CDS             5379..5996
                     /locus_tag="JN701_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7365886.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1611 a         1537 c         1444 g         1459 t
ORIGIN      
        1 gtcttgctga aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa
       61 gtaaatgccc ggcaaatacc gggcatcaag gtaattactc aaatctccac gcaatattca
      121 tgccgacacc ataatttcgt ccgggcgaag gttcgtaata tcgcccgttt gactcattga
      181 caatgacaga accaacgtat tctttatcga ataaattatc gacacgacca aataaatcca
      241 cagttaaatt gtggtaattg tatttatacc cggtgaataa gccgacgaga gtataagacg
      301 gcgcttttgc cgtattttca tcatctgcca taatatcgcc catataacgc gcttccgtgc
      361 ctgcatacca accatcttcc ggtacataac ctatcgacgc aaagcccata ttacgggcga
      421 tccctggcat ccgattaccg ttacaatcct gttcattgca aacattgctg cgataggtcg
      481 catcaagcca ggtccaggac gcgtttacgc gaaaatctcc tgcgaaacgt tgatcccatg
      541 ccagttcagc gccttgacga cgggtctttc cggcattttt gtaagtcgta cgcccaccgc
      601 tactgctatc gacaacaatt tcatcatcag tgtcggtctg aaacaatgcg agactaagca
      661 gcccatcacc aatacgcgtt ttactgccga tctcaattgt atcgttggtg gatggtttta
      721 aaccaaagtt cataccgctt tgcccatcag cacgataaga cagctcatta atcgtcggcg
      781 tttcaaaacc tcgcccggct gccagataga tattccaggc atcggtcatt gcatatttta
      841 acgaaccggc aggtagccat ttatgataac tggcatcacc gctgtcatcg ccgttacccg
      901 gagtaacgta atggtcgttg gaatcaaacc acacggagct gtagcgcacg ccagcatcca
      961 gcgacagttt ttcgctcagc tgccactgcg tctgtaaata gggatcgatg ttccacatca
     1021 gattgcgttc gtcgcgacgc aactcacctt tttgcccgta ctccggcatg ccgctattca
     1081 ggcggaagtt attgtagccc ttgcggtttt cactcatgtt ttcgtagttc aggccggtag
     1141 tgaacgtgac cggaacgccc agttcaccac ggtgtgtcca gcggctgtct atcccctggt
     1201 aatggcgttg cagggtaatc acgccgcccg catgtgacgg gttaagttgt ggtgccatgg
     1261 gtattgactg gtactgggtc gtttctcgct ctccggcata catcatcaca ctcatatcat
     1321 cttgcgcgct caggctacgc tcatagcgca acccagcctg agtttgcttg atggtttttc
     1381 gcgtgtcgta ctgttctgca cgaggcgctt gttgtggatt agccttccat tctgctttgg
     1441 ttagcccacc tgggtcatct gctttgatat ccacactatt gaaaatcaga cttaatttgc
     1501 tggcttcatc aatgcgtacg cccagtttgg cattggctaa atttttctgt gcgccactat
     1561 ggtcacgata gccgtgggtc gtaaaacgcg tggttgagac ggtgtaatcg acatcgccag
     1621 gctgtgtgcc gtctcccgtt gcgcccgttg ctttcagccc atagcgccag ctgccaaaac
     1681 tgccgtagta actactggct tcaatggttg gtggctgttg tccggtctgg gtggtgacat
     1741 tcattacccc accagacgcg ttgccataca gggcagagaa ggggccacgc agcacttcca
     1801 cattttgcac actgcttaaa tcgatgttgg atgtttgccc ttgcccgtcg ggcatggtgg
     1861 cgggaatacc gtccacatac aggcgaatac cgcgaatacc gtaagtggag cgggagccaa
     1921 atccgcgaat cgacagctgt aaatcttgcg catagttctg ccggttttgt acctgcaaac
     1981 caggcacgcc ggtcagtgat tcggacaagt taatgcgcgg tgttgccagg cgcatctcct
     2041 cgccatccac cacgcttact gctgctgggg tatccagttc tgaaaccacc tgcggtgcgg
     2101 cactgacaat catagtctgt tcatcagcgg caaaaacaac gggggaaagg acaagcagtg
     2161 cgggcaaaac ggtctgtcgg acggaaaaaa tcttcatgaa aaaagccagg ttaagaatgg
     2221 gaaaacgctg tcatggtaat aaaattgtaa acttatgcaa aacgaaacgg cacgatacgt
     2281 taattaattg agaaaattgt agtcgtaacg gcaagaaatg ctccacattt gagaaaataa
     2341 tgattaccat tcccatttat aacaagagcg tgacgatgat tacgcttagc gaagcattgt
     2401 gaagcagcaa aaatattggt tcatcaaagg gagtcgtcat gcatttacgt catctgtttt
     2461 catcgcgcct gcgtggttca ttactgttag gttcattgct tgttgcttca tcattcagta
     2521 cgcaggccgc agaagaaatg ctgcgtaaag cggtaggtaa aggtgcctac gaaatggctt
     2581 atagccagca agaaaacgcg ctgtggctcg ccacttcgca aagccgcaaa ctggataaag
     2641 gtggcgtggt ttatcgtctt gatccggtca ctctggaagt gacgcaggcg atccataacg
     2701 atctcaagcc gtttggtgcc accatcaata acacgactca gacgttgtgg tttggtaaca
     2761 ccgtaaacag cgcggtcacg gcgatagatg ccaaaacggg cgaagtgaaa ggccgtctgg
     2821 tgctggatga tcgtaagcgc acggaagagg tgcgcccgct gcaaccgcgt gagctggtag
     2881 ctgatgatgc cacgaacacc gtttacatca gtggtattgg taaagagagc gtgatttggg
     2941 tcgttgatgg cgagaatatc aaactgaaaa ccgccatcca gaacaccggt aaaatgagta
     3001 ccggtctggc gcttgatagc aaaggcaaac gtctttacac cactaacgct gacggcgaat
     3061 tgattaccat cgacaccgcc gacaataaaa tcctcagccg taaaaagctg ctggatgacg
     3121 gcaaagagca cttctttatc aacatcagcc ttgataccgc caggcagcgt gcatttatca
     3181 ccgattctaa agcggcagaa gtgttagtgg tcgatacccg taatggcaat attctggcga
     3241 aggttgcggc accggaatca ctggctgtgc tgtttaaccc cgcgcgtaat gaagcctacg
     3301 taacgcatcg tcaggcaggt aaagtcagtg tgattgacgc gaaaagctat aaagtggtga
     3361 aaacgttcga tacgccgact catccaaaca gcctggcgct gtctgccgat ggcaaaacgc
     3421 tgtatgtcag tgtgaaacaa aaatccacta aacagcagga agctacccag ccagacgatg
     3481 tgattcgtat tgcgctgtaa tagatatggc gagggtgaaa acggtgagac ttgcctgcct
     3541 tttatcacgg cgtatccggc actttcgtca gtaatctgaa tttgcccggc acgtagccgg
     3601 gcaattttgc aattaactcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3661 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     3721 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     3781 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     3841 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     3901 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     3961 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4021 gacatacaca accagtgtcg ctagaatccc ggcatacggc acatgctgac gactcatttt
     4081 cgccataaaa ctcggtgcgg aaccgcccat cgccattgag cgcagaatac gtccggtgca
     4141 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4201 gccgatatat ggcacaccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4261 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4321 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4381 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4441 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4501 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4561 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4621 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4681 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     4741 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     4801 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     4861 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     4921 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     4981 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5041 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5101 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5161 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5221 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5281 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5341 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5401 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5461 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5521 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5581 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5641 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     5701 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     5761 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     5821 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     5881 gggggcctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     5941 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatgaaatt atttgagtgg
     6001 gataccagag gattatgtta tttattattc agtaacttta tccacaatga t
//
LOCUS       DADLTD010000218         6050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLTD010000218 DADLTD010000000
VERSION     DADLTD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.220"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(25..393)
                     /locus_tag="JN701_003574"
     CDS             complement(25..393)
                     /locus_tag="JN701_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365887.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            919..1614
                     /gene="minC"
                     /locus_tag="JN701_003575"
     CDS             919..1614
                     /gene="minC"
                     /locus_tag="JN701_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY7365888.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1638..2450
                     /gene="minD"
                     /locus_tag="JN701_003576"
     CDS             1638..2450
                     /gene="minD"
                     /locus_tag="JN701_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY7365889.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2454..2720
                     /gene="minE"
                     /locus_tag="JN701_003577"
     CDS             2454..2720
                     /gene="minE"
                     /locus_tag="JN701_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY7365890.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3199..3318)
                     /locus_tag="JN701_003578"
     CDS             complement(3199..3318)
                     /locus_tag="JN701_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7365891.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3279..3464)
                     /locus_tag="JN701_003579"
     CDS             complement(3279..3464)
                     /locus_tag="JN701_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365892.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3565..3738
                     /locus_tag="JN701_003580"
     CDS             3565..3738
                     /locus_tag="JN701_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365893.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3800..4084
                     /locus_tag="JN701_003581"
     CDS             3800..4084
                     /locus_tag="JN701_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY7365894.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4088..4423
                     /locus_tag="JN701_003582"
     CDS             4088..4423
                     /locus_tag="JN701_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY7365895.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4480..>6050)
                     /locus_tag="JN701_003583"
     CDS             complement(4480..>6050)
                     /locus_tag="JN701_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7365896.1"
                     /translation="LVNNTGGDLNIISSDSILNGATKLVSGTTTLKLSENTIWNMKDD
                     SVVTHLTNSDSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRTVLGDDKSATDRL
                     IVEGNTSGSTTVYVKNAGGSGAATLNGIELITVNGDESPAEAFRQGDARIAAGAFEYQ
                     LKQQGKNWYLTSYQSVNEEDNSSEGNSEPTETPTPVLRPEAGSYVANLAAANTLFVMR
                     LNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLGADLLT
                     GGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYCAGLYATWFADDISKKGA
                     YIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQ
                     PQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPF
                     VEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAASVWGNVGVQLGDNG
                     YNDTAVMVGLKYKF"
BASE COUNT         1662 a         1396 c         1386 g         1606 t
ORIGIN      
        1 atagaacata atgtaaacaa ggcatcactc acgacacata aataacatct ttgtggtgat
       61 ttgattgatt tttccactcc gcttaccatc cacaccaaaa taacgatcat cacgacataa
      121 tttttcttta acatcgcaaa atacaccgtt agcgaatgta aattcgctgg gatcgtagcc
      181 ttgtaatgat tgtaagtttt cagcggcttt ttcgccgaga tatttccttg ttaactctgg
      241 ggaaatgcca tctgagttca cacaaatata cgggtcgcaa acaacgccgc acgccggaga
      301 ttgaagtccg ccagcgtcat gtccagacgc cattgatgag aacatcatcc ccacacctga
      361 caatccgata taaaaaatac gcatcatatt catagcacat cctgataatc caaccgtaca
      421 acacttatgc tcatttcatc atcaatccgt aatcaaaacc gctgacctgg acgagttacg
      481 atgacctgac agaagtatca gacgtcttct gcggtttcta tgattatgat agagcaattc
      541 tatggcggat attaagataa cgggtatctg ttctttaatg atgctgcaaa aaatggaaag
      601 ttaagaaagc ctggcgtggt gaaaaaatac acttgcctca atactggata tttaaacagg
      661 tctggagatg ctgtcacgat tagggtgcgg aaaaaggaag cgtaaataag aacaatcaaa
      721 agtacgacgg caatgggttg attgacaagg gtatttttta agctatgaat cagcgccatt
      781 tatcacagaa tagactttta ctctgaataa atgggagggt gacttgcctc aatataatcc
      841 agactataac atgccttata gtcttcggaa catcatcgcg cgctggcgat gattaatagc
      901 taattgagta aggccaggat gtcaaacacg ccaatcgagc ttaaaggcag tagcttcact
      961 ttatctgtgg ttcatctgca tgaggcagaa cctaaggtta tccatcaggc gctggaagac
     1021 aaaatcgctc aggcccccgc atttttaaaa catgcccccg ttgtactcaa cgtcagtgca
     1081 ctggaagacc cggtaaactg gtcagcgatg cataaggcgg tgtcggcaac cggtttgcgg
     1141 gttattggcg taagtggctg caaagatgcg caacttaaag ccgaaattga aaagatgggg
     1201 ctgcctatcc tgacggaagg aaaggaaaaa gcgccgcgtc cagctcccgc accacaggct
     1261 ccagcgcaaa atacaacgcc ggtcacaaaa acgcgtttaa tagatacccc ggtgcgttcc
     1321 ggtcagcgta tttatgctcc acaatgtgat ctgattgtta caagccacgt tagcgctggg
     1381 gccgaattga ttgccgatgg gaacattcat gtctatggca tgatgcgcgg tcgtgcgctt
     1441 gcaggggcaa gtggtgaccg ggaaacgcaa atattttgta cgaacctgat ggcggaactg
     1501 gtgtccatcg caggtgaata ctggctgagt gatcaaatcc cagcagaatt ttatggcaaa
     1561 gcggcgcgac tgcagttagt cgaaaacgct ttgaccgttc aaccgttaaa ttgatccctt
     1621 tttaacaagg aatttctatg gcacgcatta ttgttgttac ttcgggcaaa gggggggttg
     1681 gtaagacaac ctccagcgcg gccatcgcca ctggtttggc ccagaaggga aagaaaactg
     1741 tcgtgataga ttttgatatc ggcctgcgta atctcgacct gattatgggt tgtgaacgcc
     1801 gggtcgttta cgatttcgtc aacgtcattc agggcgatgc aacgctaaat caggcgttaa
     1861 ttaaagataa gcgtactgaa aatctctata ttcttccggc atcgcaaaca cgcgataaag
     1921 atgccctcac ccgtgaaggg gtcgccaaag ttcttgatga tctgaaagcg atggattttg
     1981 aatttatcgt ttgtgactcc ccggcaggga ttgaaaccgg tgcgttaatg gcactctatt
     2041 ttgcagacga agccattatt accaccaacc cggaagtctc ctcagtacgc gattctgacc
     2101 gtattttagg cattctggcg tcgaaatcac gccgcgcaga aaatggcgaa gagcctatta
     2161 aagagcacct gctgttaacg cgctataacc caggccgcgt aagcagaggt gacatgctga
     2221 gcatggaaga tgtgctggag atcctgcgca tcaaactcgt cggcgtgatc ccagaggatc
     2281 aatcagtgtt gcgcgcctct aaccagggtg aaccggtcat tctcgacatt aacgccgatg
     2341 cgggtaaagc ctacgcagat accgtagaac gtctgttggg agaagaacgt cctttccgct
     2401 tcattgaaga agagaagaaa ggcttcctca aacgcttgtt cggaggataa gttatggcat
     2461 tactcgattt ctttctctcg cggaagaaaa acacagccaa cattgcaaaa gaacggctgc
     2521 agattattgt tgctgaacgc cgtcgcagcg atgcagaacc gcattatctg ccgcagttgc
     2581 gtaaagatat tcttgaggtc atttgtaaat acgtacaaat tgatcctgag atggtaaccg
     2641 tacagcttga gcaaaaagat ggcgatattt ctattcttga gctgaacgtg accttaccgg
     2701 aagcagaaga gctgaaataa gcccgctgta aaaacgcatt tatcttcaag gcagagtcat
     2761 ttctgccttg agtttttcat ccctctcatc cacgttgtgg taaagcggcg agtattcttg
     2821 ctgatactcc tcattgctat ttccacctcc ccctttacac cttaaggctg ttcccacgca
     2881 cagaacaaaa tgtttatgcc tggttgagta aataacctca ttgttagtgt ggatacactg
     2941 ggggatgata accccgcaat ttcattattc attgttctcc cataggatga ggtttcccgc
     3001 cgtaactggc gggctttttt tgccccagaa ttttcccttt tagccccctg taaacgaaaa
     3061 ctgcgccgaa gcgcagttta atcgttgatg gtcagaacga ccagttcacg atatatactg
     3121 cgggtatcga ctgatttcga ttccatgcca ttggataaat gatagtagcg tctaagtgac
     3181 ctaagctctg ctgcaacatc agcccgcagg caccagacca ggggattcat cctgaagaga
     3241 cagcgcaggt gtattgtgtt caccactcac caaagacatc atgatgaaat gatgatatcc
     3301 cgcataagaa agaggcattt tttaaacgca gtgcgctgaa gtgtagttgg ataaaaaatc
     3361 actcttttca gataatactg tcgtacgctt atctttcgac aatgaggccg tcggcaaaat
     3421 aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa atcacacaat
     3481 aatattgata aatcagatta tcttaccttt acaacattgc gttaacagtc aatgacactt
     3541 cttgaataaa aatattgcca atcaatgagt tattcctcat ttaagattat tctcattcac
     3601 gtaaaaaaca taattcctat catcacagcc acgctcattt tgaattatct gaataatagc
     3661 gagcgttctt tagttaagca gatcttaatt gaagacgaaa ttattgtgcg tgcaacttac
     3721 ttaataccgg acatctaagt tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt
     3781 gttctgctcc acgccagcga tggcggatat gaatcgcacc acgaaaggtg cattgctagg
     3841 cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt
     3901 tggcgctggt gttggtgcag taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg
     3961 tgcaaaagta ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca atggcctcga
     4021 aggggcgatt aaaggtgccg tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa
     4081 ataaggaatg atgatgaaaa aatttgcttt actggcgggc ctgtttgttt tcgcaccaat
     4141 gacctgggcg caggactaca atattaaaaa cggtttaccg tcagaaacct atattacttg
     4201 cgcagaagcc aacgaaatgg caaaaactga cagcgcacag gttgcagaaa ttgttgcggt
     4261 aatgggtaat gccagcgttg ccagccgtga tttaaaaatt gagcaatctc cggaactgag
     4321 cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat
     4381 cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa taatatttca tatgtaccgc
     4441 gtcgatgaat acgacgcggt tttttagcta acggcgggat cagaacttat atttcaggcc
     4501 caccatcact gcggtgtcat tgtagccatt atcacccagc tgcacgccca cattccccca
     4561 gacgctggcc gctggattca ggttgccatt tacgccaagt ttaatttccc ccaaattact
     4621 cgccccatct tgttcgactt tcacaccgtt cattgagaca gcaaaatctt tactgttatg
     4681 gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc tgtttttctc
     4741 ctgagtacga ataaaggtgc ggaaaccaag tcgggtctgg atgttgttat ttgcgtcatt
     4801 ctcaatacgt gagccattgg cttcggtgtg cgcattatga tcgacgccca tccagatagc
     4861 ctgtgcctgt ggctggaata tccacgtgta tttcgccgct tccagaccaa aggatttatt
     4921 cagggcaaag ccgtaacccg cttccagcga gacggttgcg cctttcgcac tataggattc
     4981 ataggctaat tcatcccctt tcaccgagtt tttaaaccag ctatattgcg cccaggcgtc
     5041 aatgtatgcg ccttttttac tgatgtcatc ggcaaaccaa gtggcataca gccctgcgca
     5101 atagcctctg acactccctt tcgaacgata atccgacacg ctggaatgag ttgagttgta
     5161 gtcgcgggca taaccagcca tcactcccaa acgccaactg tcactgtcgg taaaaccacc
     5221 ggttaacaga tcggccccta actgcgagac gtagcgatgc gaggtcgttc tcaactgtcc
     5281 gttgctgtca cgccaggcat tatgcccgcc aatttgacgt agccaaagtc ggcttgaacg
     5341 ctcctgttca gttacaggat cgatgtagcg catttcaccc gcacggtcgt tcagacgcat
     5401 aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa cttccagctt cggggcgcag
     5461 gacgggggta ggcgtttctg ttggttctga atttccctct gagctgttat cctcctcatt
     5521 cactgactga taacttgtca aataccagtt tttgccttgt tgttttagtt gatattcgaa
     5581 agctccagct gcaatccttg catcaccttg tctgaaggct tctgctggag attcatcgcc
     5641 attcacagtt atgagttcga tcccgtttag cgtggccgcg ccgcttcctc cagcattttt
     5701 cacatagacg gtagttgaac ccgaagtatt accctcaaca ataagtctgt ccgtagccga
     5761 tttatcatca cctaatacgg tgcggatatt aagctgacca ttattaccga cataattacc
     5821 ttttacggtt aatgtttttc cttgggtaaa tgtttgacca tcatcatacg aaagattgat
     5881 aatactgtct gaattagtca gatgggtaac aacggagtca tctttcatat tccagattgt
     5941 attttctgaa agcttcagcg tggttgtgcc gctaacgagt ttagtcgctc catttagaat
     6001 agagtcggac gaaattatgt ttaagtcacc tccagtatta ttaaccagaa
//
LOCUS       DADLTD010000219         6027 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLTD010000219 DADLTD010000000
VERSION     DADLTD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.221"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            313..1188
                     /locus_tag="JN701_003584"
     CDS             313..1188
                     /locus_tag="JN701_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein"
                     /protein_id="HAY7365897.1"
                     /translation="MRKFILISMITLASVSLGACRQNATIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1196..1972
                     /locus_tag="JN701_003585"
     CDS             1196..1972
                     /locus_tag="JN701_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HAY7365898.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2141..4402
                     /locus_tag="JN701_003586"
     CDS             2141..4402
                     /locus_tag="JN701_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016536327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7365899.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4471..5646
                     /gene="senB"
                     /locus_tag="JN701_003587"
     CDS             4471..5646
                     /gene="senB"
                     /locus_tag="JN701_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HAY7365900.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
BASE COUNT         1712 a         1395 c         1391 g         1529 t
ORIGIN      
        1 attcctcccg ccggccattt tcttcgacat gtacatcagg cgcggaactc atcggggcgc
       61 gggaatgaaa tcccattcag actccggata gattcaagca agcccactta tcgtcaaaat
      121 cggtgttgca aaaacgggag tgaccataga tccgttttcc aagcggaccc cataatatcg
      181 gctggtaaac atcactcgct agtgcattct ttttgttggt aatgataatt atttacattt
      241 attctttgat gttttatcat aattaatgtt ttatcaataa atatatggaa atatattgtt
      301 tcagatggga atatgagaaa gttcatcctt atttctatga taacgttagc tagtgttagt
      361 ctgggagcct gcagacagaa tgccaccata aagcaggatg cgccagggca gaaagctttc
      421 ctgattgacg ggcagattta cgatttgcac agtggcaaaa ttatttctag cagcgagctt
      481 ttggcggatc ttgctaccgc tcgacatctt attattggtg aaaaacacga taatgccgag
      541 catcatcaga ttgagctttg gcttattcaa aatctgctca tccagcgacc acaggggagt
      601 gtgttgcttg agatgttgac gtcagaccaa cagccccgag ttaaccaagt taaatgttgg
      661 ctgaaagata atcctgtggt tcgagatagc cgtgttcagg aactacttaa ctggcaaaaa
      721 gggtggtcct gggagatgta tggcgacatc gtcatgcagc tattacgtgg gccttacccg
      781 ctgctcaatg ccaacattgg ccgggagcag atgttggcac tgtacaaaaa aaacgaattt
      841 cctaaaggaa aaaaatccac tgcacctgtg gtacaagaag ctctgcgtga aaccatcata
      901 gctatgcatg aaggcaacct tgaaagccag caactgacgt caatgctctc tgttcagcag
      961 cagcgggatc gctacatggc ccgccagctt ctgagtgccc cggtaccgtc gttgttgatt
     1021 gcaggtggtt atcacgcgtc aaaaaacatg ggcgtaccgt tacacatgga agatcttgcc
     1081 accggcaccc atccagtggt gttgatgctt gcagaaaaag gaatgaacat cacagtagat
     1141 catgctgact acgtgtggtt tgtagcgcca gatacaacaa aaaggtaaac catggatgat
     1201 gaatattctc cacttcccac agtcggtaaa atggagcagc tggtttatct gttctctact
     1261 gcttcatgga ctgattttcc ttacgtttat ctggcgtttt tccgaagttc agcccgctat
     1321 gtctcctgca ccggcgatca tgctgcaatg ggcagaaatt atagaggctc cttcttctcc
     1381 actttcgctt ccggtgggaa tcgctcaaca ggagtccgca gtgacagaag aaaagcaaca
     1441 aactgaggac agacaacaac gtcctgtcac tgaagacagt gatgcaacca ttgaaataac
     1501 taggaagaaa aagtcatccg atggagaaaa gaaaaagaca cgtccacctc gtaagataaa
     1561 ggctcaaacc agcgacagca accccacagc cgtttccagt aatgctgcac ctcaggctct
     1621 ggtcgaatcg tcccgtattg ctgcgccttt taacagtgac tcaacaaagc gagataattc
     1681 tgaggcatct tgggaaagca gagtaaaagg ccaccttaac cgttataaac gttaccctgg
     1741 cgatgccaga aaacgtgcac gaaccggcac agccgttgtg acttttaccg taaacacgga
     1801 aggcactatt gtcagtagtt tcttggaaat ctcgtcagga accctttctc ttgaccgcga
     1861 agctattgct gttttagaac gcgctcaacc acttcccaaa ccaccgcctg aaatattgga
     1921 gggaggatta tttaaagtaa aaatgccgat tacctttaag ttgaaggagt aaagggataa
     1981 ttggccgttc aagttcattt aactgaaatt taattttcat ttttatgctg aacattttat
     2041 caaacttttc cactgtcgaa aaaatcaaaa agacagtaga tcataattaa taaacaaata
     2101 gttatactta tctttattaa tattatatcc aaggaaattt atgaacgtta taaaactggc
     2161 tatcggctca ggcatattat tgctcagctg cggtgcttac tcacaatcca tcagtgaaaa
     2221 aactaattcc gacaaaaaag gagcggcaga attcagtccg ctcagcgttt ctgtcgggaa
     2281 gacgaccagt gagcaggaag ctctcgagaa aacaggcgcg accagttccc ggacaacgga
     2341 caaaaacctg caatcacttg acgcaacagt gcgtagtatg cctggtactt atactcaaat
     2401 agatcctggt cagggagcaa tcagtgtgaa tattcgaggc atgagcggat ttggtcgtgt
     2461 aaacactatg gtcgatggta ttacccagag tttttacgga acctctacct ccggaacaac
     2521 ggcgcatggt tcaactaaca atatggctgg cgtacttata gatcctaact tactggtagc
     2581 agttgatgtt acacgcggtg acagcagtgg ctctgaaggg atcaacgccc ttgccggtag
     2641 tgcaaatatg cgtactattg gcgttgacga tgtaatattt aacggtaata catatggcct
     2701 tcgttcacgt ttctctgtcg gtagtaatgg gctgggacgc agcggaatga tcgcccttgg
     2761 tggaaaaagc gacgctttta cggatacggg aagcattggc gttatggctg ctgtgagtgg
     2821 cagttctgtg tactctaatt tctcaaatgg ttctggaatt aacagcaaag agtttggtta
     2881 tgataaatat atgaagcaga accccaaatc ccaactgtat aaaatggata tcagaccaga
     2941 cgaatttaac agcttcgaac tttccgctcg aacctatgaa aataaattta cacgtcgtga
     3001 tataaccagt gacgactatt acattaaata tcattacacc cctttttctg aattaattga
     3061 ctttaacgta acggccagta ccagtcgcgg taatcaaaag tatcgtgatg gctcgctgta
     3121 tactttctac aaaacctcag cgcaaaatcg ttctgacgcg ctggatatca acaataccag
     3181 ccggttcact gtcgcggaca atgaactgga gtttatgctg ggcagcaaac tgatgcgtac
     3241 ccgctatgac cggaccattc actcagcggc gggcgacccg aaagcgaatc aggaatcgat
     3301 cgagaacaat ccgttcgcac cctccggcca gcaggatatt tcagcgctgt ataccgggct
     3361 gaaggttacg cgcggcatct gggaggcaga tttcaatctc aactacacac gtaacaggat
     3421 cacagggtac aagcccgcct gcgattcacg cgttatctgc gtgccacagg gtagctacga
     3481 tattgacgat aaagagggtg gcttcaaccc ttcagttcag ctttctgctc aggtaacacc
     3541 atggcttcag ccgttcattg gctacagcaa atccatgcgc gccccgaaca tccaggagat
     3601 gttcttctct aattcaggag gcgcatccat gaacccattc ctgaagcctg aacgtgcaga
     3661 aacctggcag gcgggtttta acattgatac cagagattta ctggtcgaac aggatgccct
     3721 gcgctttaag gctctggcgt accgcagcag gatccagaac tacatctaca gcgagtctta
     3781 tctggtttgt tctggaggtc gtaaatgcag tatggctgag gtgattggca atgactggga
     3841 gggcattagc gatgaataca gcgacaatat gtacatctac gttaactcgg caagcgacgt
     3901 tattgcaaag ggcttcgaac tggagatgga ttatgatgca ggttttgctt ttggccgact
     3961 ctctttcagc cagcagcaaa cagaccagcc aacctccatc gccagcacct actttggcgc
     4021 aggggatatg accgaactgc ccagaaaata catgacgctg gatactggtg ttcgcttctt
     4081 cgataacgcg ttgaccctgg gcactatcat aaaatacaca ggcaaggctc gtcgcctgtc
     4141 gcctgatttt gagcaggacg aacataccgg cgcaataatc aaacaggatt tgccgcagat
     4201 cccaacgatt atcgatctct atggtactta cgagtacaac cgcaacctga cactgaaact
     4261 ttcggtacaa aacctgatga acagagatta ttcggaggcg ctgaataagc tcaacatgat
     4321 gccaggtctt ggtgacgaga gccacccagc caattccgcg cgtggcagaa catggatatt
     4381 tggcggggac attcgtttct gatgacaatt tccgccaggt tcagacaata cgttttctct
     4441 cttatgtcaa ttttattgca ggaacgaaaa atgaatattt tcactttatc caaagcaccg
     4501 ctatacttgt taatttcact atttttaccc acgatggcca tggctatcga tccacctgaa
     4561 cgcgaacttt cgcgatttgc cctgaaaacg aattaccttc agtcccctga tgaaggcgtc
     4621 tatgaactgg cgtttgataa tgccagtaaa aaggtgtttg cagcagtcac cgatcgtgta
     4681 aatcgtgaag ccaataaagg ctatctgtat tcgtttaatt cagattcgct gaaagtcgaa
     4741 aataaataca cgatgccata ccgggcattt tcgctggcga taaatcagga taaacatcag
     4801 ctctatatcg gacacaccca gtcagcgtcc ctgcgtatca gtatgtttga caccccaacc
     4861 ggtaaactgg taagaaccag cgacaggtta agttttaaag cggcaaacgc tgcagattcg
     4921 cgttttgagc atttgcgcca tatggtttac agccaggatt ccgataccct gtttgtgagt
     4981 tatagcaata tgctgaaaac ggccgagggc atgaagcctc tgcataagct gttaatgctc
     5041 gacgggacga cgcttgcctt aaaaggcgag gttaaggatg cttacaaagg tacagcgtat
     5101 ggtctgacga tggatgaaaa aacacagaaa atctacgttg gcggaagaga ttacatcaac
     5161 gaaattgatg cgaaaaatca gacgctgctg cgtaccatcc cgttgaaaga tccgagacca
     5221 caaatcacaa gtgtgcagaa tctggcggtg gactccgctt ctgaccgtgc ctttgtggtg
     5281 gtattcgacc atgacgatcg ttccggtaca aaagatggcc tctatatttt tgacttacgc
     5341 gacggtaaac agcttggcta tgtgcacaca ggagccggag ctaacgcggt gaaatacaat
     5401 ccgaaatata acgaactgta tgtcaccaac ttcactagcg gcaccatcag cgtagtggat
     5461 gccaccaaat acagcatcac ccgtgaattt aacatgccgg tctacccaaa ccagatggtg
     5521 ttgtcggacg atatggatac cctttacatt ggcatcaaag aaggctttaa ccgcgattgg
     5581 gatcctgatg tgtttgtgga aggagctaaa gaacgtattc tgagcattga tttgaaaaag
     5641 tcgtgatcgg gggaaaaaac atgggataaa aactccgtta ttccactaaa ctggccggac
     5701 taggaaaagc aaacatctct gtcctgccgg tacgcctgtt acgccgctcc atgaggctga
     5761 ggaatctcca gaaaacgaga caggcacagc attgtacgat caactgattt gtgccaataa
     5821 ttcaatgaaa tcacagctat gcctgttcac gtataggaaa ttgaaaaact aagcgatacc
     5881 actgtccgcg tcttatgcct cgtgcggtaa gctgtttttc acatcacgcc ggtgtttttt
     5941 tctttctgtg gacggtttta atactgtaat cggccgtttt ttatacttac cccgattccc
     6001 gggcgcactt atcctgcatc accccgg
//
LOCUS       DADLTD010000220         5905 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLTD010000220 DADLTD010000000
VERSION     DADLTD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.222"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..297)
                     /locus_tag="JN701_003588"
     CDS             complement(<1..297)
                     /locus_tag="JN701_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365901.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKH"
     gene            complement(707..1078)
                     /locus_tag="JN701_003589"
     CDS             complement(707..1078)
                     /locus_tag="JN701_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365902.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(1139..1636)
                     /locus_tag="JN701_003590"
     CDS             complement(1139..1636)
                     /locus_tag="JN701_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365903.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1712..2500)
                     /gene="aadA1"
                     /locus_tag="JN701_003591"
     CDS             complement(1712..2500)
                     /gene="aadA1"
                     /locus_tag="JN701_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7365904.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2558..3082)
                     /gene="sat2"
                     /locus_tag="JN701_003592"
     CDS             complement(2558..3082)
                     /gene="sat2"
                     /locus_tag="JN701_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY7365905.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(3177..3650)
                     /locus_tag="JN701_003593"
     CDS             complement(3177..3650)
                     /locus_tag="JN701_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY7365906.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3982..4518
                     /locus_tag="JN701_003594"
     CDS             3982..4518
                     /locus_tag="JN701_003594"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HAY7365907.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4633..4959
                     /locus_tag="JN701_003595"
     CDS             4633..4959
                     /locus_tag="JN701_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7365908.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(5147..5386)
                     /locus_tag="JN701_003596"
     CDS             complement(5147..5386)
                     /locus_tag="JN701_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY7365909.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1542 a         1411 c         1321 g         1631 t
ORIGIN      
        1 atgtttggac gccttggcaa aaattagagc ctgcttgaag tgcaggcgag cacgtgcttg
       61 gacgattgat ccattcgcgg tcaaaaactc aatcttggat gacagcgtgt caggaaatcc
      121 aatatcgaag tcccgccccg tgtcaaagta gggcatttca tgatcaaagg acgaagcttc
      181 ccaggccact tccctaatat tccagccgct agaaacaaga tttcgtacga cttcatcaac
      241 ttttttgctt tccaacttgt cacaaagtgc ataggcaaga gctttggcgg cagacacgtg
      301 cgtttcctct taaacatagg tcgaacggat acccgaggca aagcggagca ttagtcccaa
      361 agcatctccg gtagatccgg aggctccata ctgaaggcat ggcatcttgg gcattgatat
      421 ttgaaacttc tctctagata gcagggattt cttggccttg cctcatgctg atagcttgta
      481 aatatcacac gatccttagc cccacaaagt gagcatactc cgtagctttc atttatagaa
      541 atcgtcgtta gattgtcgca gcatgtgcac gttccgacta tttgctcagg aagcatcgcc
      601 ataccttggc ctaggtaaag aagatcagac tcgtagccac atcggtggca actgagcttg
      661 tacgcaatgc ccatctggtt gtctcacctg aatcgtaacg tcagagctat tcatccatcc
      721 attcggcatc aatgtatgcc actaagccga ccagctgacc gtttggactg aagtatgttg
      781 cctgcacccc acttgacatc tcatcaatac caccgcactg cgctaggcgc cggtcccact
      841 tgcccacaga agcgtcagga tcggctgagt tccccaagac ccttgtgagc catgcctcct
      901 ttaactcaac ctctcgaatg attgcgtcgg ccagctcctc cgcgttatct agttcgacat
      961 agtctggaat tcggacgtac gttctgaaca cagctggata cttacttggg cgattgtcat
     1021 acatgacatc aacaatgtac ccgtttgtgt aaccgtctct tggaggttcg tatgacacta
     1081 gtggttcccc tcagcttgcg actagatgtt gaggcctaac attttattag agagcaggct
     1141 agttgcttag atacatgatc ttcaggccgt tatctgtcag ggcaagcgaa aattggccat
     1201 ttatgacgac caatgccccg cagaagctcc catctttgcc gccatagacg ccgcgccccc
     1261 cttttggggt gtagaacatc cttttgccag atgtggaaaa gaagttcgtt gtcccattgt
     1321 tggcaatgac gtagtagccg gcgaaagtgc gagacccatt tgcgctatat ataagcctac
     1381 gatttccgtt gcgactattg tcgtaattgg atgaactatt atcgtagttg ctctcagagt
     1441 tgtcgtaatt tgatggacta ttgtcgtaat tgcttatgga gttgtcgtag ttgcttggag
     1501 aaatgtcgta gttggatggg gagtagtcat agggaagacg agcttcatcc actaaaacaa
     1561 ttggcaggtc agcaagtgcc tgccccgatg ccatcgcaag tacgaggctt agaaccacct
     1621 tcaacagatc gcgcatagtc ttccccagct ctctaacgct tgagttaagc cgcgccgcga
     1681 agcggcgtcg gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc
     1741 ctttcacgta gtgaacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     1801 gtccaagata agcctgccta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
     1861 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
     1921 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
     1981 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
     2041 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
     2101 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
     2161 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
     2221 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
     2281 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
     2341 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
     2401 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
     2461 atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac tcctgaatta
     2521 agccgcgccg cgaagcggtg tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg
     2581 agaaccagta ccagtacatc gctgtttcgt tcgagacttg aggtctagtt ttatacgtga
     2641 acaggtcaat gccgccgaga gtaaagccac attttgcgta caaattgcag gcaggtacat
     2701 tgttcgtttg tgtctctaat cgtatgccaa ggagctgtct gcttagtgcc cactttttcg
     2761 caaattcgat gagactgtgc gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt
     2821 gttcgataga ggctagatcg ttccatgttg agttgagttc aatcttcccg acaagctctt
     2881 ggtcgatgaa tgcgccatag caagcagagt cttcatcaga gtcatcatcc gagatgtaat
     2941 ccttccggta ggggctcaca cttctggtag atagttcaaa gccttggtcg gataggtgca
     3001 catcgaacac ttcacgaaca atgaaatggt tctcagcatc caatgtttcc gccacctgct
     3061 cagggatcac cgaaatcttc atatgacgcc taacgcctgg cacagcggat cgcaaacctg
     3121 gcgcggcttt tggtacaaaa ggcgtgacag gtttgcgaat ccgttgctgc cacttgttaa
     3181 cccttttgcc agatttggta actataattt atgttagagg cgaagtcttg ggtaaaaact
     3241 ggcctaaaat tgctggggat ttcaggaaag taaacatcac cttccggctc gatgtctatt
     3301 gtagatatat gtagtgtatc tacttgatcg atcaggcttt tgtatatctc cccaccacct
     3361 gaaacaatga catgatccgt tattttcttt aggttggtta aagcatcttt aattgatgga
     3421 aagatcaata cgttctcatt gtcagatgta aaacttgaac gtgttacgac cgcatacttt
     3481 cggttgggta atgctcccat tgattcaaaa gtcttgcgtc caaccaacag ccattggtta
     3541 taggtaatag ctttaaacag gagctgttca cctttggcac tccatggaat atcagggcca
     3601 ttcccgataa ctccattctt cgatatagct accattagtg atagtttcac aattcttcct
     3661 cagaggttaa cattttatta gtaagcatgc tcgttttgtc acccgctgat gcttaccgtt
     3721 aattaatata atcacttgta ttaaatagac tttctttaaa aatacaagac actctgttat
     3781 tacaaatcgt gcatgccgtc tatcctgctt gcacgatgca cattatctca cccaaagaac
     3841 tcataacatt ttaatttatt tagcattaaa ttaaaactct accggtaaac aagcatctct
     3901 aggcgtaaaa aacaggcact tttttaaatc ccagctagaa taggctgtat aggcagacag
     3961 ttgcaagaca aggagcgttt tatgtctaac agtccatttt taaattctat acgcacggat
     4021 atgcgacaaa aaggttatgc gctgaaaact gaaaaaactt acctgcactg gattaagcgt
     4081 tttattctgt ttcacaaaaa acgtcatcct cagaccatgg gcagtgaaga ggtcaggctg
     4141 tttttatcca gcttagcaaa cagcagacat gtagccataa acacgcagaa aatcgcttta
     4201 aatgccctag cttttttgta caacaggttt ttacaacagc cgttgggcga tattgattat
     4261 atccctgcaa gcaagcctag acggctaccc tctgttatct ctgcaaatga agtgcaacgc
     4321 attttgcagg ttatggatac tcgcaaccaa gttattttta cgctgctgta tggtgcaggt
     4381 ttgcgcatta atgaatgctt gcgtttgcgg gttaaagatt ttgattttga taatggctgc
     4441 atcactgtgc atgacggtaa gggtgggaaa agcagaaaca gcctactgcc cacgcgccta
     4501 atcccagcaa taaaataact cattgagcaa gcgcggctta ttcagcaaga cgacaactta
     4561 caaggcgtag ggccatcgct gccttttgct ttagatcaca aatacccttc tgcttatcga
     4621 caagcggcgt ggatgtttgt ctttccctcc agcacgctct gcaaccaccc gtataacggc
     4681 aaattatgcc gccatcatct gcatgactcc gttgcgcgaa aggcattgaa ggcagccgta
     4741 caaaaagcag gcatcgttag caagcgtgtc acttgtcata catttcgtca ctcgtttgct
     4801 acgcatctat tacaagcggg gcgtgatatt cgcactgtgc aagaactctt agggcataac
     4861 gatgttaaga ccacgcaaat ctatacgcat gtgttgggtc agcattttgc cggcaccacc
     4921 agtcctgcgg atggactgat gctacttatc aatcagtaaa agtagataaa tatctttatg
     4981 tagccctgag tagcccctga tactttttat ggtgaaaaag ataaacaatc atagagggga
     5041 taaatatgag ccagttctac cattgttcag ataagtttaa gcaagatact gtcaatcaca
     5101 gtgaccattc atggtcatta cacttcctcc agttgccaga caatgcctaa cataaaaacg
     5161 caaaagccca aaagtcagag cttaggtgtt ctaggcgttt tctaagcgct gaagaaatac
     5221 gtcttaccgt agattgcgtg cgtttctgaa gcacattttc cttatacatg gctttatccc
     5281 aggcagcttc atcaagctct tggagtaaca agccagtaat aatgcgacac tctcgggttt
     5341 gtagcgagcc gctggtgaga tcggagttat agcggaaagg ggtcatttgt aattatttgt
     5401 gggtggagga tagttaactt attattgtag ttggaagagg ctgctttcca aacattagca
     5461 cctcgtaatg cttgttactg tatattaatt tatgctctta gcgcatgtta atgggctatt
     5521 acaatgcata atgtccacat caagataact gtggcttaac acctgtacaa gcgaaaaaaa
     5581 tcaatcacct gtccggaaat acgggatcac tgtaaagtgt tggtgaggca gtctaggagg
     5641 gggatttaat ttgggatact gatgaaagga ttatgatcag ttggatatag gatcgtacta
     5701 agctgagcgg caggttagct agattagtta accagataag tgaaatctag ttccaaacta
     5761 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt
     5821 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta
     5881 ttttgtccgc ccacagggct gccga
//
LOCUS       DADLTD010000221         5894 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLTD010000221 DADLTD010000000
VERSION     DADLTD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.223"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            380..988
                     /locus_tag="JN701_003597"
     CDS             380..988
                     /locus_tag="JN701_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY7365910.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1086..2477
                     /gene="selA"
                     /locus_tag="JN701_003598"
     CDS             1086..2477
                     /gene="selA"
                     /locus_tag="JN701_003598"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY7365911.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2474..4318
                     /gene="selB"
                     /locus_tag="JN701_003599"
     CDS             2474..4318
                     /gene="selB"
                     /locus_tag="JN701_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7365912.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4508..5659
                     /gene="yiaY"
                     /locus_tag="JN701_003600"
     CDS             4508..5659
                     /gene="yiaY"
                     /locus_tag="JN701_003600"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY7365913.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1395 a         1489 c         1613 g         1397 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atttctcatc cttgttttat cgggttattt
      181 gtctgctgac aggcaaaacc ctacagattt ataagaaagc ctgaagtgag atatccatca
      241 gagtgaaata attaggaaaa tatttatatc accatgataa atatgtgatt tatttggaag
      301 taacaaacca tcaacatttt ctgccctttt tcccatcacc aggccatact gatatttttc
      361 ctcgcacaaa ggactcggca tgaaactcgt cggtagctac accagcccgt ttgtacgcaa
      421 actttctatt ctgttgttag aaaagggcat aactttcgaa tttattaatg aactgcccta
      481 taacgcggac aacggcgtgg cgcaatttaa cccgttagga aaagtgccgg tgctggtgac
      541 cgaagagggc gaatgctggt ttgattcgcc gatcatcgct gaatatattg agttaatgaa
      601 tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc gcaaaattga
      661 ggcgctggcg gatggcatta tggatgccgg gctggtatcg gtgcgtgaac aggcgcgtcc
      721 ggcggcgcag cagtctgaag atgaattgtt acgccagcgg gagaaaatca accgcagtct
      781 ggatgtgctg gaaggatatc tggtcgatgg cacactcaaa accgatacgg tcaatctggc
      841 gactatcgcc attgcctgtg ctgtcggata tctcaatttc cgccgcgttg cgccaggttg
      901 gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc gcgaaagttt
      961 tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc catctcacgt
     1021 tacaatccgt ggttatgtta aacgcccttc tccatgtgag agggccttga ttagccaggt
     1081 ttcctatgac aaccgaaacg cgttccctct atagtcaact tccggctatt gatcgcttat
     1141 tgcacgatag ctccttcctt tctttgcgtg atacttatgg tcacacccgc gtggtggaat
     1201 tgttgcgtca gatgctcgac gaagcgcgag aagtgattcg tgacagccag acgcttcctg
     1261 cgtggtgtga aaactgggcg caagaagtcg atgcccggtt gacgaaagag gcgcagagcg
     1321 cgctgcgtcc ggtgatcaac ctgacgggaa ccgtgctgca taccaacctt ggacgagctt
     1381 tacaggcgga agccgcggtg gaagccgttg cgcaggctat gcgttcgcca gtgaccctcg
     1441 agtacgatct ggacgacgct ggacgcggac atcgcgatcg ggcgctggcg caactgctgt
     1501 gccgtattac gggggcggaa gatgcctgta tcgtcaataa caatgcggcg gcggtgttat
     1561 tgatgttggc ggccactgcc agcgggaaag aggtggtagt atctcgcggc gaattggtgg
     1621 agattggcgg cgcgtttcgt attcccgatg ttatgcgtca ggcaggctgc accctacacg
     1681 aagtagggac caccaaccgc acgcacgcga atgattatcg tcaggcggtg aatgaaaata
     1741 ccgcactgtt gatgaaagta cataccagta actacagcat tcaggggttc accaaagcga
     1801 tagatgaagc ggaactggtg gcgctcggca aagagctgga tgttcccgta gtgactgatt
     1861 taggcagtgg ctcgctggtc gatcttagcc agtacggttt gccgaaagag ccaatgccgc
     1921 aggagttgat tgcggcgggc gtcagtctgg tgagcttctc cggcgacaag ttgttaggcg
     1981 ggccgcaggc agggattatc gttggtaaaa aagagatgat cgcccgcctg caaagccacc
     2041 cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa gccacgttgc
     2101 gtctttattt acaccctgaa gctctgagtg aaaaattacc gaccctgcgc ctgcttaccc
     2161 gcagcgcaga ggtcattcaa atccaggcac aacgtttaca ggctcccctt gccgcacatt
     2221 acggcgcgga gtttgcggta caggttatgc catgtctttc gcagattggc agtggttcgc
     2281 tgccggttga tcgcctgccg agcgcggcat taacgtttac accccatgat ggacgcggta
     2341 gccaccttga gtcattagcc gcccgctggc gtgaattgcc agtgccggtg attggtcgta
     2401 tttatgacgg acgattgtgg ctggatttac gctgccttga agatgagcaa cggtttttgg
     2461 agatgttgtt gaaatgatta ttgcgactgc cggacacgtt gaccacggca aaacaacctt
     2521 attgcaggcg attactggcg taaatgctga ccgtctgccg gaagaaaaaa agcgcggcat
     2581 gaccatcgat ctcggctatg cctactggcc gcagccggat ggtcgcgtgc ctagttttat
     2641 cgacgtaccc ggtcatgaaa agtttctttc caacatgctg gcgggcgttg gtggtatcga
     2701 tcacgcgctg ttggtggtgg cgtgcgatga cggcgtgatg gcgcagaccc gtgagcatct
     2761 ggcgattttg cagctgaccg gtaacccgat gctgacagtg gcgctgacca aggccgatcg
     2821 cgtggacgaa gcgcgtgttg atgaggttga acgccaggta aaggaggtgc tgcgggaata
     2881 cggttttgct gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg gaattgatgc
     2941 cctgcgcgag catctgcttc agttaccagg acgtgaacac gccagccaac atagtttccg
     3001 cctcgcgatc gaccgcgcat ttaccgtaaa aggtgccggg ctggtcgtca ccggtacggc
     3061 gttaagcggg gaagtgaagg taggcgattc actctggctg actggtgtaa ataaaccgat
     3121 gcgtgtacgt gcgctgcatg cacaaaacca gccaacagaa accgcccatg ctgggcagcg
     3181 tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag attaaccgtg gcgactggct
     3241 gcttgccgat gcgccgccag agccgttcac acgggtgatt gtcgagcttc aaacccatac
     3301 accgctgacc cagtggcagc cgctgcatat tcaccacgcc gccagccacg tcacgggacg
     3361 cgtttcactg ctggaagata accttgctga actggtcttc gacaccccgt tatggctggc
     3421 agataacgac cgcctggtat tgcgcgatat ctctgcccgc aacacgctgg ccggagcgcg
     3481 cgtcgtgatg cttaacccgc cgcgtcgcgg taaacgtaaa ccagagtatc tgcaatggct
     3541 ggcttccctc gccagggcac agaatgatag cgaagcgtta gccgttcacc tgcaacgcgg
     3601 cgcggttaat cttcctgatt tcgcctgggc gcgccagctc aacggcgaag ggatgcgcga
     3661 actgctgcaa cagcctggct atattcaggc tggttatagc ttgttgaatg cgccggttgc
     3721 cgctcgctgg caacggaaaa ttctcgacac attagcgact tatcatgagc aacatcgcga
     3781 tgaacctggg cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg aagatgaagc
     3841 gctagtactg ttgctgattg aaaagatgcg cgaaagcggc gacatcctca gtcatcacgg
     3901 ttggctgcat ctgccggatc acaaagcggg cttcagcgaa gagcagcagg ccatctggca
     3961 aaaagcagag ccgctgttta gtgacgagcc gtggtgggtg cgtgatctgg caaaagagac
     4021 gggtaccgac gagcaggcaa tgcgcttgac gctaagccag gcggcgcagc aaggaataat
     4081 taccgcgatc gtcaaagatc gttattaccg taacgatcgg attgtcgaat ttgccaatat
     4141 gatccgcgat ctcgatcagg agtgtggttc aacctgcgcg gcggatttcc gcgatcgctt
     4201 aggcgtaggc cgaaagctgg caattcagat tctggaatat tttgaccgca ttggctttac
     4261 gcgtcgtcgt ggaaatgatc atttattgcg cgacgcatta ttatttccgg aaaaataagg
     4321 aaatgattaa ttaattttta aaataaatta atacaaattt cttatgattt taaaaaaagc
     4381 acattgttta atgaatacaa tgtgcttttt attagattaa ttttgataat taataataat
     4441 gatcatgttt tcgaatgaaa atcctcagta agcttcccac ccttttttac actttcagga
     4501 gtgtgttatg gcatcttcaa ctttctttat tccttctgtg aatgtcatcg gcgctgattc
     4561 attgactgat gcaatgaata tgatggcaga ttatggattt acccgtacct taattgtcac
     4621 tgacagtatg ttaacgaaat taggtatggc gggtgatgtg caaaaagcac tggaagaacg
     4681 caatattttt agcgttattt atgatggcac ccaacctaac cccaccacgg aaaacgtcgc
     4741 cgcaggtttg aaattactta aagagaataa ttgcgatagc gtgatctcct taggcggtgg
     4801 ttctccacac gactgcgcaa aaggtattgc gctggtggca gccaatggcg gcgatattcg
     4861 cgattacgaa ggcgttgacc gctctgcaaa accgcagctg ccgatgatcg ccatcaacac
     4921 cactgcgggt acggcctctg aaatgacccg tttctgcatc atcactgacg aagcgcgtca
     4981 tatcaaaatg gcgattgttg ataaacatgt cactccgctg ctttctgtca atgactcctc
     5041 tctgatgatt ggtatgccga agtcactgac cgccgcaacg ggtatggatg ccttaacgca
     5101 cgctatcgaa gcatatgttt ctattgccgc cacgccgatc actgacgctt gtgcactgaa
     5161 agccgtgacc atgattgccg aaaacctgcc gttagccgtt gaagatggca gtaatgcgaa
     5221 agcgcgtgaa gcaatggctt atgcccagtt ccttgccggt atggcgttca ataatgcttc
     5281 tctgggttat gttcatgcga tggcgtacca gctgggcggt ttctacaacc tgccacacgg
     5341 tgtatgtaac gccgttctgc tgccccatgt tcaggtattc aacagcaaag tcgccgctgc
     5401 acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg acaggtaaaa acgacgcgga
     5461 aggtgctgaa gcctgcatta acgccatccg tgaactggcg aagaaagtgg atatcccggc
     5521 aggcctacgc gacctgaacg tgaaagaaga agatttcgcg gttctggcga ctaatgccct
     5581 gaaagatgcc tgtggtttta ctaacccgat ccaggcaact cacgaagaaa ttgtggcgat
     5641 ttatcgcgca gcgatgtaat catcatttcc acaacggctg gcaaattgtc agccgctttt
     5701 tcaactatct ctgtaaccct tgcccgtaaa ttcgtgatag ccgtcgtaaa gctgttaccg
     5761 actggcgaag atttcgccag tcacgtctac ccttgttata cctcacaccg caaggagacg
     5821 atcatgacca ataatccccc ttcagcacag attaagcccg gcgagtatgg tttccccctc
     5881 aagttaaaag cccg
//
LOCUS       DADLTD010000222         5821 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.224, whole genome shotgun sequence.
ACCESSION   DADLTD010000222 DADLTD010000000
VERSION     DADLTD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.224"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..392)
                     /locus_tag="JN701_003601"
                     /pseudo
     CDS             complement(1..392)
                     /locus_tag="JN701_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(578..3886)
                     /gene="icsA"
                     /locus_tag="JN701_003602"
                     /gene_synonym="virG"
     CDS             complement(578..3886)
                     /gene="icsA"
                     /locus_tag="JN701_003602"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY7365914.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAVPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4415..5617
                     /gene="virA"
                     /locus_tag="JN701_003603"
     CDS             4415..5617
                     /gene="virA"
                     /locus_tag="JN701_003603"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY7365915.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1805 a         1318 c          964 g         1734 t
ORIGIN      
        1 caggctgtac gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta
       61 ggatcttcac cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg
      121 ttactaattt atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct
      181 acttggaaat gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt
      241 acagaactac tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt
      301 gctcgacctt gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga
      361 ggcgcataac cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct
      421 gtgtaacgcc aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga
      481 tcgcaggcat accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga
      541 gaaatgcagg acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca
      601 aaataccttg ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga
      661 tgcttaggtt atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt
      721 tgcctatctg gtgatttgac atgccattca taattacacc atattgcttt gagctccatt
      781 tccaattcac ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactat
      841 gactaattaa ggatgtacgc aatcccaatt ttgcttgaat attattgacc ccagaggatg
      901 tgaccatagt gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct
      961 ggacttgtgg attgagatac aatgcgttgt caatattatt atgagtagtc cagcgtatgg
     1021 ttgggtagat atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac
     1081 cagttaaccc atctcctttg actgtattat taaaccaacc gtactgcaac caagcatcca
     1141 tatatagacc tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat
     1201 aaccgctcac agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct
     1261 gatatccata agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct
     1321 tcaaaatatc tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat
     1381 cagctaaatt atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga
     1441 gctgacctgt agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa
     1501 acatcgtgtt cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt
     1561 gaatactacc ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac
     1621 tcatttgagt agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag
     1681 ttagatacca cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat
     1741 aactaccagc aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg
     1801 aaataatttg aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa
     1861 cataggtaat accagacgca tggcctgtaa tttccagccg gtcagtttta ctgttgtcat
     1921 tgccgagaac cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa
     1981 cataggtctg accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca
     2041 gagtcatgtt acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga
     2101 ttagcacagt cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca
     2161 aggtagcacc tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca
     2221 tttttagaat gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa
     2281 tgagagttcc ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat
     2341 ggctgagatt atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga
     2401 actctccatg actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct
     2461 ggtcactgat atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga
     2521 tagatatatt agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt
     2581 ccttctgcgc ccagttattt ataatagctt ccacttttac attgttgcca aatgacatat
     2641 ttccagaagc ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag
     2701 aaacgtcacc tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag
     2761 cacataaatc accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca
     2821 gaatggatga attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta
     2881 atgaattgtt tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat
     2941 cttctttacc tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag
     3001 catcccccca atgtccacca ttaccgccta aaatagctcc attgttgatt atagacaggt
     3061 tatctcctgt gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc
     3121 cgttgcctcc agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat
     3181 cacctccatt accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag
     3241 tccctttgtt gattatgggt agattgactc ctgtgacggc atcacctcca ttccccccat
     3301 cactatcact ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa
     3361 acagattgtc tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc
     3421 cgctaccacc aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt
     3481 caccaccatt accgccacaa ctattaccat cattattatc accagcacca ccatgacctg
     3541 cgctaatcat aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg
     3601 aagaagttat ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat
     3661 caacaggtgt taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg
     3721 aaagaggaac agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag
     3781 gagaaaaaga caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct
     3841 gtgaacattg ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca
     3901 gtaagtggtt gataaacccc taaaaagatt ccgttttatc cggaataaag ggacgatata
     3961 tgcaaaaaca tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg
     4021 caacgggaga aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc
     4081 gataataaat taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt
     4141 tctgtacgct tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct
     4201 gccccttgat gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct
     4261 accagactct ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt
     4321 aaagagaaga ataacattcc attttattat gttcccaatt taattatatt aatatatatt
     4381 ggcatcaata ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca
     4441 tgaaagaaat gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa
     4501 taaattgagt ttttgtgacg ttttactgaa aataataact ttcggaatat attctccaca
     4561 cgaaactcta gccgaaaaat atagcgaaaa aaaactaatg gatagtttct caccatcact
     4621 atcacaagat aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg
     4681 gctatgcctt aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc
     4741 cactcaactt tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt
     4801 caacttagga aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa
     4861 gcctgcacca gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga
     4921 tgattacttc cccgttaaag acactgattt taatagtgtt tggcatgata tttacagaga
     4981 tattcgagca tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact
     5041 aaaattaatt gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa
     5101 agagcttcaa atgctaagtt acaaccaatt aaataaaata atcaattcaa acttccccca
     5161 acaagaccta tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc
     5221 atttatatcc gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt
     5281 ggaacacata tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc
     5341 tgaacaacga gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa
     5401 aaacgacact gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt
     5461 accatcgcac ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt
     5521 tcatgctgga acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg
     5581 tcaagtatca tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag
     5641 actgtaaaaa aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat
     5701 ggggaccaat agaatctcag cccatccttt ttatatcatt atgaaccgcc ccggaaatcc
     5761 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaataca cgtttttccc
     5821 c
//
LOCUS       DADLTD010000223         5815 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLTD010000223 DADLTD010000000
VERSION     DADLTD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.225"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..856
                     /gene="pdeC"
                     /locus_tag="JN701_003604"
     CDS             <1..856
                     /gene="pdeC"
                     /locus_tag="JN701_003604"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY7365916.1"
                     /translation="FALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYY
                     QPIIDIKTEKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTDYVIDNVFRDL
                     GDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEK
                     MTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIA
                     EHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLP
                     ARELTRGQ"
     gene            complement(859..1182)
                     /gene="soxS"
                     /locus_tag="JN701_003605"
     CDS             complement(859..1182)
                     /gene="soxS"
                     /locus_tag="JN701_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY7365917.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1268..1732
                     /gene="soxR"
                     /locus_tag="JN701_003606"
     CDS             1268..1732
                     /gene="soxR"
                     /locus_tag="JN701_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY7365918.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2278..3627
                     /locus_tag="JN701_003607"
     CDS             2278..3627
                     /locus_tag="JN701_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7365919.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3779..5428
                     /locus_tag="JN701_003608"
     CDS             3779..5428
                     /locus_tag="JN701_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY7365920.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5582..>5815)
                     /locus_tag="JN701_003609"
     CDS             complement(5582..>5815)
                     /locus_tag="JN701_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7365921.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1257 a         1376 c         1603 g         1579 t
ORIGIN      
        1 ttttgcgttg cccgccggta ttttggggag tcttgttctg ctattactct ggctacgtat
       61 tcgacaaaac tatttgtctc ccaaacgcaa attgcaacgc gctctagaaa aacatcaact
      121 ttgtctttat taccagccaa taatcgatat caaaacagaa aaatgtatcg gcgctgaagc
      181 gttgttacgt tggcctagtg agcaggagca agtaatgaat ccggcagagt ttattccgct
      241 ggcagaaaag gaggggatga tagaacaggt aactgattat gttattgata atgtcttccg
      301 cgatctgggc gattacctgg caacacatgc agatcgctat gtttctatta acctgtcggc
      361 ctccgatttt catacgtcac ggttgatagc gcgaatcaat cagaaaacag agcaatacgc
      421 ggtgcgtccg cagcaaatta aatttgaagt gactgagcat gcatttcttg atgtcgaaaa
      481 aatgacgccg attattctgg ctttccgcca ggctggttac gaagtggcaa ttgatgattt
      541 tggtattggc tactctaact tgcataacct taaatcattg aatgtcgata ttttgaaaat
      601 cgataaatcg tttgttgaaa cgctgaccac ccacaaaacc agtcatttga ttgcggaaca
      661 catcatcgag ctggcgcaca gcctggggtt aaaaacgatc gctgaaggcg tcgaaactga
      721 ggagcaggtt aactggctgc gcaaacgcgg cgtgcgctat tgccagggat ggttttttgc
      781 gaaggcgatg ccgccgcagg tgtttatgca atggatggag caattacccg cgcgggagtt
      841 aacgcgcggg caataaaact acaggcggtg gcgataatcg ctgggagtgc gatcaaactg
      901 ccgacggaaa acgcgggaga aggtctgctg cgagacataa cccaggtcca ttgcgatatc
      961 aaaaatcgga cgctcggtgg tgcgcaactc aacggcggcc agtaacaggc gacgttggcg
     1021 aatgtaatcg ccaagcgtct gatgcgtcac cgtgcggaac attcgttgca agtaccactt
     1081 tgaatagcct gatttttttg cgactacatc aatgttaagc ggctggtcaa tatgctcgtc
     1141 aatccatgcg ataagatcct gaataatttt ctgatgggac ataaatctgc ctcttttcag
     1201 tgttcagttc gttaattcat ctgttgggga gtataattcc tcaagttaac ttgaggtaaa
     1261 gcgatttatg gaaaagaaat taccccgcat taaagcgctg ctaacccccg gcgaagtggc
     1321 gaaacgcagc ggtgtggcgg tatcggcgct gcatttctat gaaagtaaag ggttgattac
     1381 cagtatccgt aacagcggca atcagcggcg atatagacgt gatgtgttgc gatatgttgc
     1441 aattatcaaa attgctcagc gtattggcat tccgctggcg accattggtg aagcgtttgg
     1501 cgtgttgcca gaagggcata cgttaagtgc gaaagagtgg aaacagcttt cgtcccaatg
     1561 gcgagaagag ttggatcggc gcattcatac cttagtggcg ctgcgtgacg aactggacgg
     1621 atgtattggt tgtggctgcc tttcgcgcag tgattgcccg ttgcgtaacc cgggcgaccg
     1681 cttaggagaa gaaggtaccg gcgcacgctt gctggaagat gaacaaaact aaagcgccac
     1741 aagggcgctt tagtttgttt tccggtcttt gtctttctct ctatcccgct ggtacacagg
     1801 agggtttccc ccgacgtcaa cacacctcat tcgagcacgt ggtggaggtt ccggttggtg
     1861 ttgatgcttt aattgtatgt caccgacgtt tcttcgccag tgtaaaagta tactttttaa
     1921 ccgcaatatt tttgtcatct cagacgattt tttatcgcaa tcctgaacgg tatacggctc
     1981 gataacgctg caatcttgcg caccgacgat aacgtttgcg catcaattgc ctgttttttc
     2041 atcgtcaaga caataaaaga gaaaaaagca gcaaacttcg gttgaaaaag ccgctatgat
     2101 cgccggataa tcgtttgctt tttttaccac ccgttttgta tgcgcggagc taaacgtttg
     2161 cttttttgcg acgcagtaaa ttgtcgcaaa cctggagcag gaagataacg tttcgctggc
     2221 aggggattgt ccgccacgca tcttgacgaa aattaaactc tcaggggatg ttttcttatg
     2281 tctacgccat cagcgcgtac cggcggttca ctcgacgcct ggtttaaaat ttcacaacgt
     2341 ggaagcactg tccgtcagga agtggttgcc gggttaacaa cgtttctggc gatggtctac
     2401 tcggtcatcg tcgttccagg tatgttgggt aaagcaggct tcccgcctgc ggcagttttc
     2461 gttgcaacct gtctggttgc cggactcggt tctatcgtga tgggtctgtg ggctaatctg
     2521 ccgttggcga ttggttgcgc catctccctg acggcgttta ccgcattcag cctggtgctg
     2581 gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt tcctgatggg tgtgctgttt
     2641 acggtaattt ctgccacggg tatccgtagc tggattttgc gcaacttgcc gcacggtgtg
     2701 gcgcacggca cggggatcgg tatcggtctg ttcctgctgc tcattgccgc taatggtgtt
     2761 ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg cgctgggtga tttcgcgacc
     2821 ttcccggtga ttatgtcact ggtaggtctg gcggtgatca tcggcctgga aaaactgaaa
     2881 gtccctggtg gcattctgct gaccattatc ggtatctcaa ttgtcggttt gatcttcgat
     2941 cctaacgtcc atttctccgg cgttttcggc atgccttcat tgagcgatga aaacggcaat
     3001 tcactgattg gcagcctgga cattatgggc gcgctgaatc ctgtagtcct gccaagcgtt
     3061 ctggcgctgg tgatgacggc agtatttgat gccaccggaa ctatccgtgc cgtcgccggc
     3121 caggcgaacc tgctggataa agatgggcag atcatcgacg gtgggaaagc actgaccact
     3181 gactccatga gcagcgtttt ctctggcctg gtgggtgctg ctccggcagc ggtatacatc
     3241 gagtctgcgg cgggtacggc ggcgggcggt aaaaccggtt tgacggctat caccgttggc
     3301 gtgctgttcc tgctgattct gttcctctct ccgctctctt acctcgttcc ggggtatgca
     3361 acggctccgg cgctgatgta cgttggcctg ctgatgctga gcaacgtggc gaaaatcgac
     3421 tttgctgatt ttgttgatgc gatggcgggt ctggttacgg cggtattcat cgtgctgacc
     3481 tgtaacatcg taacaggcat catgatcggc ttcgcgactc tggtgattgg tcgtctggtt
     3541 tccggcgaat ggcgcaagtt gaacatcggt acggtcgtta tcgccgtggc gctggtgacc
     3601 ttctatgcgg gtggctgggc tatctaatct ttcatcggat tctgaaaacg ggtggcaatg
     3661 gctgcccgtt tttattttct ccgcacattg tgtgagcttt tgcgatattc tgaaaaaatg
     3721 agaattcagg cataacgcct ttctcagatc ataaaaaaac atcgcaaaca gggaacgcat
     3781 ggaaatcttc ttcaccatac tgataatgac cctcgtggtc tcgctgtccg gggtggtcac
     3841 tcgtgtcatg ccctttcaga tcccgcttcc gcttatgcaa atcgccatcg gtgcgctact
     3901 ggcgtggccg acgtttggtt tgcatgtgga gtttgatcct gaactctttt tagtcttgtt
     3961 tatcccgccg ttgctgttcg ctgatggctg gaaaacgccg acccgtgaat ttctcgaaca
     4021 tggtcgagag attttcggcc tcgcgctggc gctggtggtg gtcaccgtgg tcggcattgg
     4081 cttccttatt tactgggtgg tgccgggcat tccgctgatc cccgcctttg cgctggcggc
     4141 ggtactttct ccgaccgatg ctgtggcgct ctccggaatt gttggcgaag ggcgcatccc
     4201 gaaaaaaatc atgggcattt tgcagggcga agcgttgatg aatgacgcct ccggcctggt
     4261 gtcgttgaag tttgccgtgg cagtggcgat ggggacgatg atcttcaccg tcggcggtgc
     4321 gacggtcgaa tttatgaaag tagccattgg tggtattctt gccggttttg tggtgagctg
     4381 gctgtatggt cgctcgctgc gattcctcag ccgctggggc ggtgatgaac ccgcgacgca
     4441 gatcgttctg ctgttcttgc tgccattcgc ttcttatctg attgccgaac atattggcgt
     4501 ttccggcatc ctcgctgcgg ttgccgccgg gatgaccatc acccgctccg gtgtgatgcg
     4561 ccgtgcgccg ctggcaatgc gcctgcgcgc aaacagcacc tgggcgatgc tggaatttgt
     4621 ctttaacggc atggtgttcc tgctgttagg tctgcagctg ccgggtattc tggagacgtc
     4681 gctgatggcg gcagaaatcg accctaacgt cgaaatctgg atgctgttta ccgatattat
     4741 tctgatatat gcggcgctga tgctggtccg tttcggctgg ctgtggacga tgaaaaagtt
     4801 cagcaaccgc ttcctgaaga agaagccgat ggagtttggt tcgtggacca cacgagaaat
     4861 cctgatcgcg tctttcgccg gggtgcgtgg ggcgatcact ctggccggtg tgctctctat
     4921 cccgctgctc ttgccggatg gtaacgtctt cccggcgcgc tatgagctgg tgttcctggc
     4981 ggctggcgtc attctcttct cgctgtttgt cggtgtggtg atgttgccta ttctgctaca
     5041 acacattgaa gtcgcggatc attcgcaaca attgaaagag gaacgtattg cgcgagcggc
     5101 aacggcagaa gtggcgattg tggcgatcca gaaaatggag gagcgtctgg cggcggatac
     5161 cgaagagaat atcgataacc agctgcttac ggaggtcagt tctcgcgtca ttggtaacct
     5221 gcgtcgtcgc gccgatggac gtaacgacgt tgaaagttcc gtgcaggaag agaaccttga
     5281 gcgtcgcttc cgtctggcgg cattgcgttc tgaacgtgct gaactgtacc acctgcgcgc
     5341 cacgcgggag atcagcaacg aaacgctgca aaaattactg cacgatctcg atttgcttga
     5401 agcgttgcta attgaggaaa atcagtaagc cgaaagcgac gtaaccaggc ccgaggtaat
     5461 ctgaaaaagg tgcaatcttt tgcaccttta acaggttagc tcgggctaac attcatgatt
     5521 ctaaaacaaa taagttatct ctcttgtaaa gtcaattcat tggctgttct tcgacttaac
     5581 ctcaatctgc gtgttgcata atatctgcag taatcttcat agcgactgca ttaatattat
     5641 cctcagtttg tgacatgcca gtatgagagg tacaatcttc ttcaaagaat ttctgtagtg
     5701 ttaccagggt atcactatca atggggatat tttcctctcc acggagtatg gaaatgccca
     5761 ttccctggac gctagcctca tcaaaattaa cccgatagag acgggtatta aacat
//
LOCUS       DADLTD010000224         5709 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLTD010000224 DADLTD010000000
VERSION     DADLTD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.227"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1826)
                     /locus_tag="JN701_003610"
     CDS             complement(<1..1826)
                     /locus_tag="JN701_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7365922.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWGSVNA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGN"
     gene            1871..2200
                     /locus_tag="JN701_003611"
     CDS             1871..2200
                     /locus_tag="JN701_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAY7365923.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            2187..2579
                     /gene="tnpB"
                     /locus_tag="JN701_003612"
     CDS             2187..2579
                     /gene="tnpB"
                     /locus_tag="JN701_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7365924.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            2586..4187
                     /locus_tag="JN701_003613"
     CDS             2586..4187
                     /locus_tag="JN701_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HAY7365925.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(4322..4582)
                     /locus_tag="JN701_003614"
     CDS             complement(4322..4582)
                     /locus_tag="JN701_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365926.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(4630..4839)
                     /locus_tag="JN701_003615"
     CDS             complement(4630..4839)
                     /locus_tag="JN701_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAY7365927.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(4935..5309)
                     /locus_tag="JN701_003616"
     CDS             complement(4935..5309)
                     /locus_tag="JN701_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365928.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(5324..>5709)
                     /locus_tag="JN701_003617"
     CDS             complement(5324..>5709)
                     /locus_tag="JN701_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365929.1"
                     /translation="LAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDK
                     TLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCV
                     VTDSAEKAQSVTSVECTVTVSAAAG"
BASE COUNT         1280 a         1733 c         1445 g         1251 t
ORIGIN      
        1 tcattaccca gccccagcag acggttgaat tccgtatgct gaccgttcat aaccagcatg
       61 gactggtaca ccttattctg ctctgccgcc tgctgacgaa ttaacgccac acgacggtct
      121 tccagcccgg caagcacatc ctgaatggac tgcgcttttt cctgcatcag tgccagacgg
      181 gactgctcaa cggcaagctg ctctgttgcc tgagcaagcc cttccgttac ggtcttcacc
      241 gaggtcagat ggtttatcat gaatccgtca ccggtcgtcc agcccgggtt cgccagaaca
      301 tactgatatc ctgcgatttt ttcctgcagg gatttcaccc gactagcctg ttcatcaatc
      361 agccggttct gctctgtcag cgccgcccgt gttcgtcctt cattatctga ggcttcaggc
      421 aaagacattg acggcgtttt atgcgcgatt tcatctatcg tcagtgcata ctggcgcgca
      481 gactcccagg cctgctcctg attctggtac agcgtgtacc atgctgcagc ccccagcatc
      541 accagtccgg gtacgccacc aaccagcccc agcgcaccgc tcatcagacg tgagcccacc
      601 gccgttgtac tgttcagcgc attctgggcg gcgcttctgg cagcaatatt tctgttcagg
      661 cgttcctgtg tggccgccag acgggcctct gcagcaatct gcatctccgt cccgcgggct
      721 gccgccacgg cctgctgagc acggtacacg gctgcccttg cccgcgccgt ggcaatctgc
      781 gttcccctga actgtgcttc cgccagtgca acttcattac gtgcagccgt cacaagtcct
      841 gccgtggcag acatcgctcc ggaggccata ttgccaaagt accgggcaac cccgacggca
      901 accagcgccc ccacagctgt tgccacatta tcaatctgtc cggcaacacc gttcagcacg
      961 ccggagagcg tttttgtcac cccgcaggcc tcattcactt cgcccaccca ggccataaag
     1021 gcgttttcca ccttcgtgat actaccggaa accgtttccg gcatggccgc atattcatca
     1081 cgtaatatcc ccagctggct gattaacgca gggaccactt tatccgctgt cagttttccg
     1141 tcatccgcca ttgccttcag atctttacgg gccacaccca tccccgcagc aagcgcgcgg
     1201 atcacccggt cgccattttc gttaacagca ttaaattctt cgccacgcag tacaccctgt
     1261 gccagtgcct ggctgaactg ggtgatcacc gaaccggatt ccgcaactcc agcccctgac
     1321 agtttcagtc ctgtcgaaat ggcctccgtc accttcagca catcatcagc actgtaacca
     1381 tattcacgca tcgaggcagc cgaacgggca aacagggccg cattatccga aaacgcggta
     1441 cctgttcgct gactgatatc catcagcact ttctgtgatg acgagaattc atccgatgac
     1501 tgagatgcct gtttcagacg ggcattcacg gaaccccact catcggccag cgaaatcagg
     1561 tgtccggtgg caaaggcacc ggcaaatgcg ccagccattc cgacagcaga accgcgaatt
     1621 tccgtcaact gactgttcag ttctgcaagg gcgcggcgct gttcccgtgc ggcagcagcg
     1681 gcatgacgtc cgccattctg cagggtccgg taatattcac tgcccatacg ggacgcccgc
     1741 tggatctccg actggaatga ctgtgaattt gccgaaattt tgagtgtcgt tacggggaag
     1801 tcatcttcac agggatcttc ggtcagttag tattaccgac atccattcac caacttaccg
     1861 gagataatcg atgtcaaaac cccgatggac cattgaacag aaaaggcagc acgttgctgc
     1921 ctggcgcgcc agtggtctta cacgtcagca atactgtgaa ctcaacgata ttcccttcac
     1981 atcacttcgc gaatggccgc aggatgtcgc aaaagctgaa cgccgggcaa atgaaccgac
     2041 cgttcttccc gttcatattg cgccaccact gcatgctgat gtaccacagc ccgtcacgaa
     2101 tgagccagtc acgctcttcc ttcccggtgg agtacggatg tgctgtcagc cgtcacaact
     2161 taccgatgtc ttcagggcac tcaaatatgc tcagtcctga taatgtcttc atcgcaatta
     2221 aacctgttga catgcgccgg ggcatcgact cactgacgca gtatatacag gatgaactcc
     2281 ggtcgacatg gcatgaggga gccgccttcg tctttgttaa caaagtccgt tcgcgtatca
     2341 aagttctccg gtgggataaa cacggggtgt ggttgtgtac ccgccgtctg cacaaaggca
     2401 gcttccgctg gccacgtgca aatgacgctg cctggcacct cactcccgac gagtttaact
     2461 ggctgattgc cggtgttgac tggcagcagg ttaagggaca tgacctgacg aaatgggtct
     2521 ggcagaatga acctgaactg cgccctgaaa acacgcaaaa tacactgcta actcagtgaa
     2581 aaataatgaa tatccgtatc tggagtggta tactcccctg tatggatatc tccgctctca
     2641 acaccacgaa tgacatcgaa aaactgcgtg ctatggcact tgccatggta caagaagtca
     2701 tgtcggagaa tgccgaaaaa gagcgggaat tactggagaa aagccggcgc atccagcttc
     2761 tggaagaaat gctgaaactg gttcgtcaac agcgcttcgg aaaaaaatgt gaaacgctgg
     2821 ctggtatgca acgctcccta ttcgaagagg atgttgatgc cgatatcgcc gcgcttaccg
     2881 cacatctgga taaactgctc ccgcaatccc ctgaagaaga tgaaaaagcg tcccgttcac
     2941 gcccgatacg caaaccctta ccggttcatc ttccacgggt ggaaaaaatg atccagccgg
     3001 acactgacca ttgccctgaa tgtgacgagc cgctgcacta tatccgcgat gcggtgagtg
     3061 aaaagctgga gtatattccc gctcactttg tggtgaaccg ttatgtccgt ccgcaataca
     3121 gttgtccctg ttgccagaag gtgttcagcg gtgaaatgcc ggcacatatc ctcccgaaaa
     3181 gtgccgttga gccatcagtc atcgcacagg tgatcatcaa taaatacggt gaccacctgc
     3241 ctctgtatcg ccagcaacag gtctttgccc gttcagatgt cgggctgccc gtcagttcga
     3301 tggctgacat ggttggcgcg gcgggtgccg cattatctcc cctggcggcg ttactccatc
     3361 gcgagttgat aaaccgtccg gtggtgcatg cagatgagac taccctgaag atcctgaaca
     3421 cgaagaaagg cggtaaatcc tgctccggtt atctgtgggc atacgtcagt ggagaaagga
     3481 cgggaccgtc agttgtgtgc ttcgactgcc ggaccggacg tagccatgag tatcctgaaa
     3541 actggcttca gggctggggc gggacgctgg ttgtcgacgg acataaagct taccggactc
     3601 tggcaaacaa agtgccggag atcacgctgg ccggatgctg ggcccatgcc cgcaggggct
     3661 tcgccgacct gtataaaatc agtaaagatc cacgggctgc catagccgtg aagaaaatcg
     3721 cggggttgta ccgtcttgag aagaagatca gtagccgccc cgtggaaaaa atccgccagt
     3781 ggcgacagcg ttatgcccgt ccgatactgg aagaactgtg gtcatggctt gaagagcagg
     3841 aaccgcaatg ttctccggga aaggcattac acaaagctat tgcctatgcg ctgtctcatc
     3901 gcgtggaact gagccgcttc ctggaagatg gtgcggtgcc gctggataat aatgtgtgtg
     3961 aacgggccat caaaaacgtg gttctgggca gaaaatcgtg gctgttcgcc ggttcgcaga
     4021 tggcgggaga acgcgccgcg caaataatga gcttgctgga aaccgcgaaa cgcaacggtc
     4081 tggagccgca tgcctggttg acagacgtcc tgatgcgtct gccggagtgg ccggaggagc
     4141 gactggcaga gttgctgcct cttgagggat ttaccttctc cgggtgagtg atacctgccg
     4201 tcaggtgttc gtgcaccggg ccataacctg cagtcgggag ttgaactcct gacggcagga
     4261 aatgagccag accagcaacc acgcgggctg acagcgaata agaacctgaa tcagcccgga
     4321 gtcattcccg ggtcagtaca tcatcacctg caattaatcc ccgagtcagt acttcgcggc
     4381 ctgacgcgat cacgctactc agtacttcag agcccgcctc cggtacctgc ctggcgatcg
     4441 cctctgtgca acaaaataaa tcaaccgtta tcaaaccacg agcgttcgta tgcgccgccg
     4501 atgacgcgcg cctgaaaaac gcgtcaatat gagttccccg gaagcttaca attttgataa
     4561 tcagttcacg taacgtcgcc attcaccttt ctccgggcgt aaaaaaaccg cctcagcggt
     4621 tctcatcatt catgactgtg ctgcaaagct cagcgcgtct tccagcgccg caaacggatc
     4681 cacctccggc ttatcctcat cctcgcccca gcagagcatg acgtccttca gtgcaacatt
     4741 catcccctgt gccccgaaaa ccgctttcac gatctgtgca ttacggatat ccccgcgctc
     4801 atcacccagc ggggatatcc tgtcgaactc catccacatc atcgcctcgc tcgcactcag
     4861 gctgtggcgc agttcggata aggtgcgccc cagacggagc gcaagtcgca tcagaaagcg
     4921 aatttccggg cgggctactt ttttctggcc gactctgcat cagcgatcag ttccagtgcc
     4981 tgacgcagca accgggcatg taccggacca tagacggcca gcacctgctc acggtcgtcc
     5041 ggagtgaaca cccgctgcag gtccgtatca cacaggacat cgcagaacag cgtcacatcc
     5101 gcttccaagt tacggcgggt tttcgccacc accgacaggg tatcgtcatc ctctccatca
     5161 ccattgagca cttcctgcca cagataccag gcctctgccg aaggctcccg cagcaccacg
     5221 ctgacattcc cccattccgg caccttcacc gttttatgac gaaaccctga cagtctggcc
     5281 agcgccagcg ttttcagatc ctttttcatg atgacccatc cccttatccg gcagctgcgc
     5341 tcactgtcac ggtgcattca acagacgtca cactctgtgc tttctctgcc gaatcggtca
     5401 ccacgcaggt atatttcccc gcatcagcgg actgcgcacc tggcttactg aaggtgtctg
     5461 tcgtctgccc gtcaaccggc tgaccatcct tcttccaggc gtatttatac ggcggcattc
     5521 ccccgttggc actgactgac attgtcagca gcgcaccggt attcacggta agtgtcttat
     5581 ccagattttt cacaaacgcc agcggtacca caaaggacac cggtttgcct ttcagacgca
     5641 gtgagaacgt tgcagccacc acaccgttgg taccggatga ccaggtgtgc tgacgcactt
     5701 ccgccagga
//
LOCUS       DADLTD010000225         5672 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.228, whole genome shotgun sequence.
ACCESSION   DADLTD010000225 DADLTD010000000
VERSION     DADLTD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.228"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            4..246
                     /locus_tag="JN701_003618"
     CDS             4..246
                     /locus_tag="JN701_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365930.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            360..998
                     /locus_tag="JN701_003619"
     CDS             360..998
                     /locus_tag="JN701_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7365931.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            995..1732
                     /locus_tag="JN701_003620"
                     /pseudo
     CDS             995..1732
                     /locus_tag="JN701_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            1732..3828
                     /locus_tag="JN701_003621"
     CDS             1732..3828
                     /locus_tag="JN701_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY7365932.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3875..4153)
                     /locus_tag="JN701_003622"
     CDS             complement(3875..4153)
                     /locus_tag="JN701_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365933.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4423..4821
                     /gene="psiE"
                     /locus_tag="JN701_003623"
     CDS             4423..4821
                     /gene="psiE"
                     /locus_tag="JN701_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY7365934.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4865..>5672)
                     /locus_tag="JN701_003624"
     CDS             complement(4865..>5672)
                     /locus_tag="JN701_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7365935.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1327 a         1483 c         1576 g         1286 t
ORIGIN      
        1 gttatgaaaa aagttctgta tggcattttt gccatatctg cgcttgcggc gacttctgcg
       61 tgggctgcac ctgtacaggt gggcgaagcg gcagggtcgg cagcaacgtc ggtttcggcg
      121 ggtagttcct ccgcgaccag cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg
      181 gcaaccggtg gcggtgatgg ttctaatacc gggaccacca caaccacgac caccagtacc
      241 cagtaataaa gtatgtatcc ccaaaataat ttgagtcatt gcatctgtgg cttgaagtat
      301 gaagggattt accataacca cactccggtg tggttattct gcccctctgg agaagagtcg
      361 tgaagcgacc tgcactcatt cttatctgcc tgctattaca ggcctgttca gccacgacta
      421 aagagctggg caattcactg tgggacagtc tgttcggcac gccaggcgta cagctgacgg
      481 acgatgatat tcaaaatatg ccctacgcca gccagtacat gcagcttaat ggcgggccgc
      541 aattgtttgt ggtgctggcc ttcgctgaag acggacaaca aaaatgggtc actcaggatc
      601 aagccactct tgtcacacaa catggtcgtc tggtgaagac tttgcttggc ggcgacaacc
      661 tgattgaagt gaataacctt gccgccgacc cgctgattaa acccgcacaa attgttgatg
      721 gtgcaacatg gacgcgcacg atgggctgga ccgagtacca gcaggtacgc tacgccaccg
      781 cacgctcagt cttcaaatgg gatggcaccg ataccgtcaa agtcggcagc gatgaaaccc
      841 tggttcgcgt gctggacgaa gaagtctcca ccgaccaggc gcgctggcat aaccgctact
      901 ggatcgacag cgaagggcaa attcgccagt cggaacagta tctcggcgcg gattatttcc
      961 cggtgaaaac cactctcatc aaggcggcaa aacaatgatt aaacaaactt ttgtcgcgtt
     1021 gcttttgagt gtgggagcgt catcggtctt cgcggcagga accgtcaagg tgttcagcaa
     1081 tggcagcagt gaggccaaaa cactaacagg cgcagagcat ttaatcgatc tggtaggcta
     1141 accgcggctg gcaaacaact ggtggcccgg tgcggtgatt agcgaagagc tggcaacggc
     1201 ggcagcattg cgtcagcagc aggcgttgct gacacggctg gctgaacagg ccgcagactc
     1261 cagcgccgac gatgccgccg ccattaacgc cttacgccag caaattcagg cgttgaaagt
     1321 gacgggcagg caaaaaatca atcttgatcc cgatatcgtg cgcgttgccg aacgcggtaa
     1381 cccaccgttg cagggcaact acacgctgtg ggtcggacca ccgccgtcca cggtcacgtt
     1441 gttcgggctt atcagccgtc ctggcaagca gccattcact ccaggtcgcg acgtggcgag
     1501 ctatctctcc gggcagaacc tgcttagcgg tgcggatcgc agctacgcgt gggtggttta
     1561 cccggacgga cgcacgcaaa aagcgccggt ggcttactgg aacaagcgtc acgtagagcc
     1621 gatgcccggc agcattattt atgttggcct cgcggactcc gtctggagtg agacgcctga
     1681 tgcccttaac gccgacattc ttcagactct gacgcagcgg ataccccaat aatgaaaaaa
     1741 agacatctgc ttagcttact ggcgctgggc attagcatgg cttgctacgg cgaaacatat
     1801 cctgcgccca ttggcccgtc gcagtcggat ttcggtggcg taggattatt acaaacgccc
     1861 accgcacgca tggcgcggga aggggagttg agcctgaact atcgcgataa cgatcagtac
     1921 cgttattact cagcttcagt gcaactcttc ccgtggctgg aaacaacgct gcgctacacc
     1981 gacgtgcgca cccggcagta cagcagcgtc gaagcgttct ctggcgatca aacgtataaa
     2041 gataaagcct tcgatctcaa actgcgtttg tgggaagaga gttactggct gccgcaagtg
     2101 gcggttggtg cgagggatat cggcggtacg gggctgtttg atgcggaata tcttgttgcc
     2161 agcaaagcct gggggccgtt cgattttacg ctcggcctgg gctggggtta tctgggcacc
     2221 agcggtaatg tgaaaaatcc gctctgttca gccagtgata aatattgcta tcgcgataac
     2281 agctacaaac aggcgggatc tatcgacggt agccagatgt tccacggtcc tgcctcactg
     2341 tttggcggcg tggaatacca gacgccctgg caaccgctgc gcctgaaact ggagtatgaa
     2401 ggcaataatt atcagcagga ttttgccggg aagctggagc aaaaaagtaa gtttaacgtc
     2461 ggtgcgattt atcgcgttac cgattgggcc gacgttaacc ttagctatga acgtggcaac
     2521 acctttatgt ttggcgtcac gctgcgcacc aactttaacg atctgcgccc gtcttacaac
     2581 gataacgccc gcccgcaata tcaaccgcag ccgcaggatg ccattttgca gcattcggtg
     2641 gtggcgaatc agttaacgct gttgaaatac aatgtcggac ttgccgatcc gcagatccag
     2701 gcgaaaggcg atacgctgta tgtcaccggc gagcaggtga aatatcgtga ttcgcgcgaa
     2761 gggatcatcc gtgctaatcg gatcgtgatg aacgatctgc cggatgggat caaaacgatc
     2821 cgcattacgg aaaatcgtct taacatgccg caggtgacga cggaaaccga tgtcgccagc
     2881 ctgaaaaatc atctcgccgg agagccgttg ggccacgaaa cgaagctggc gcaaaaacgc
     2941 gtcgagccag tggttccgca gtccaccgag cagggctggt atatcgacaa atcacgcttt
     3001 gatttccata tcgatccggt gctgaaccag tcggtcggtg gcccggaaaa cttttacatg
     3061 tatcagctgg gcgtgatggg aatggcagat ttgtggctga cggaccatct gctgaccacc
     3121 ggcagcctgt ttgcaaatct tgccaacaac tacgacaagt ttaactacac caatccgccg
     3181 caggactcgc acttaccgcg cgttcgtacc catgtgcgcg agtatgtgca gaacgatgtc
     3241 tatgtgaata acctgcaagc caactacttc cagcatctgg gcaacggctt ctacggtcag
     3301 gtctatggag gttatctcga aaccatgttt ggcggtgcgg gggccgaagt gttgtatcgt
     3361 ccgctggaca gtaactgggc gtttggtctg gatgccaact acgttaaaca gcgcgactgg
     3421 cgtagtgcaa aagatatgat gaaattcacc gactacagcg tgaaaaccgg acatctgacc
     3481 gcctactgga cgccatcttt cgcccaggac gtgttggtga aagccagcgt cgggcagtat
     3541 ctggcagggg ataaaggcgg cacgctggag atcgccaaac gctttgacag cggcgtggtg
     3601 gtgggtggct atgccacgat caccaacgtt ttgaaagagg agtacggcga aggggacttc
     3661 accaaaggcg tgtatgtctc ggtaccgttg gatctcttct cgtctggccc gacacgcagc
     3721 cgtgcggcga ttggctggac gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag
     3781 ttccagttgt atgacatgac cagcgaccgt agcgtcaatt tccgctaagt catgggaaag
     3841 gtgccagttt tcgcactcac tggcaccttc attcttaatg gcctgtcgag ccatcaggaa
     3901 cgcagttcac ccccggtttg cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt
     3961 ccgtcgatat cggttgcagg tctggttgcc tgagcgactg ggccatcatt gggctggaga
     4021 cattgctttg cggtaggtcg gctttatcag cagtaccgag cggaccagca taagcaggaa
     4081 gaacagagac tgataacatc aaagcagcaa aataaggctt catttttacc acctttatca
     4141 ggttacgttt catttgttcc agaggaacat tgtcgatttt tcgcgcattg ctggtggctg
     4201 ggaatcacct gaatgggtga tttttgaatt accggcttgg gtgcggtttg tcttgccgga
     4261 tgcgccgcca ggcgcggctt atccggccta cgggtaggta tatccggctt tggtggaggc
     4321 gcgctccaaa tccaggttga acaaaacata cacaaaaaat atagatctcc gtcacatttt
     4381 tgcgttatac aggaagctcg ccactgtgaa ggaggtactg ctatgacgtc actctctcgt
     4441 ccgcgcgtgg agtttatctc caccattttg cagaccgtac tcaatcttgg cctgctgtgc
     4501 ctcggcctga ttttggttgt cttcctcggc aaagaaacgg tgcatctggc agatgtgctg
     4561 ttcgcgccag aacaaaccag caaatatgag ctggtggttt actttctcta tttcgaattt
     4621 atcgcgctga ttgtgaagta ctttcagtcc ggttttcact tcccgctacg ttactttgtc
     4681 tatattggga tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc gccactggat
     4741 gtgttgatct actcggctgc gatcctgtta ctggtgatca ctctgtggct gtgtaattcg
     4801 aaacggctga agcgggagta aaaagacagc acgccgaact ggcgcggcgt gctggacagg
     4861 aagattacag cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag agggcttcca
     4921 gctcctcaag ggttttacct ttggtttccg gggcaaattt ccacataaat agtgctgcca
     4981 gaacgcccat acacccgtaa atccagtagg agaaaccgtt gtggaaatgg gccaccagcc
     5041 aggagttttt gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc agccactggg
     5101 ccgccaccgc gattgccagc gccttaccac gaatagcatt cgggaagatt tccgacagca
     5161 gtacccagca taccggaccc caggacatgg caaaggcagc aacatagaac agcatcgaca
     5221 gtagcgccac aatacccggt gcctgagtgt aaaacgcggt accgaggcta aacataccga
     5281 ttgccattcc gagtgcgccg ataatttgca gtggcttacg accaaattta tccaccgtca
     5341 taattgccag aacggtgaag gtgaggttga taactccgac aataatggtc tgcaacagcg
     5401 cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac agcaccacat
     5461 tgatgccgac aaattgctgg aagatggaga gcataacgcc gatgacaatc acgcccacgc
     5521 caaacatcag cagacgacca ccggttttgc ggccatgatc cagggagtgt ttaatttcct
     5581 gtactgcctg agttgcaagc gtgttgccca taattttgcg caggatactt tccgcctgtt
     5641 cttgcttgcc gcgtgacatc agccagcgag ga
//
LOCUS       DADLTD010000226         5477 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLTD010000226 DADLTD010000000
VERSION     DADLTD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.230"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..94
                     /locus_tag="JN701_003625"
     CDS             <1..94
                     /locus_tag="JN701_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7365936.1"
                     /translation="LNAVLDYIDAVAATDTSTAPDVIWPELPEA"
     gene            complement(66..503)
                     /locus_tag="JN701_003626"
     CDS             complement(66..503)
                     /locus_tag="JN701_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7365937.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNLWLDYLDALELIDTSSAPDIEWPTPPAVQAR"
     gene            complement(505..891)
                     /locus_tag="JN701_003627"
     CDS             complement(505..891)
                     /locus_tag="JN701_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365938.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            922..1488
                     /locus_tag="JN701_003628"
     CDS             922..1488
                     /locus_tag="JN701_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7365939.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1631..2803
                     /locus_tag="JN701_003629"
     CDS             1631..2803
                     /locus_tag="JN701_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HAY7365940.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2813..3328
                     /locus_tag="JN701_003630"
     CDS             2813..3328
                     /locus_tag="JN701_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAY7365941.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            3383..3685
                     /locus_tag="JN701_003631"
     CDS             3383..3685
                     /locus_tag="JN701_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY7365942.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            3700..3819
                     /locus_tag="JN701_003632"
     CDS             3700..3819
                     /locus_tag="JN701_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAY7365943.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            3812..>5477
                     /locus_tag="JN701_003633"
     CDS             3812..>5477
                     /locus_tag="JN701_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7365944.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTL"
BASE COUNT         1403 a         1360 c         1529 g         1185 t
ORIGIN      
        1 acttaacgcc gtgttggatt acattgacgc ggtggcggca acagatacca gcaccgcgcc
       61 ggatgtcatc tggcctgaac tgccggaggc gtaggccatt caatatctgg agcactggag
      121 gtatcaatca gttccagtgc gtccagataa tccagccaca aattatattg cgccagttcc
      181 tcacctttca gacgaccaat agcggcttta ccgggccatt gtttactgtt catgtattcg
      241 ttggcctgat taagcaataa ttgcctttct gattctgcct gttgaataag ttcttcatat
      301 gatggtggag gtattagtgc ccatgtgggt aatccatttt ttcctgcaac acgaattttg
      361 tcatctgggg gcgtattgat tgcaaattca ttataaacat catcactgac agccagagca
      421 tcatctggcc atgaatttgc attaatgtaa tcatccttaa gcgcaggatt cacaaaaatg
      481 tttaaagatg gactataaaa catattaaac ccctatagcg atataacgac ctaatacagc
      541 atttgcagca gtcgctatgc ttgaaaaacc gcggaactga tttgatgtaa ttgcagaagc
      601 cgacaggatt cctgcgcctg aaggtgtatg ccccacatga ctaactatca tcccataaca
      661 tgctgacgga aaggcaaaag gaaaatcatt aagatatcct gcatcttcac caccagaccc
      721 accaaatctc gcctgccccc actgaataat cagtgttctc cgggaacctg aaataattaa
      781 cggaatcgtt acatacccat tcaggccaat gacacccgat gcagtgccag ccagagataa
      841 ttctcccaaa ccaacgttta agaaaatgca gagataatgg ctaactggca tcatccccgg
      901 tttttattca ggggattgat catgcttatt ggctatgtac gcgtgtcaac aaatgaccag
      961 aacaccgatt tgcaacgtaa tgcactgaac tgcgcgggat gtgagcggat ttttgaggat
     1021 aaaatcagtg gcactaagcc cgacagaccg gggcttaaaa aactgctcag gacactatcg
     1081 gcaggagaca cgctggttgt ctggaagctg gacagactgg gacgcagtat gcggcatctt
     1141 gttacgctga tagaagagtt gcgccagcgt ggtgtgaatt tccgaagcct gactgacagt
     1201 attgatacca gtaccccaat gggccgtttc ttttttcacg tcatgggggc tctggctgaa
     1261 atggaacgtg aactgatagt tgaacgtacc agagcagggc tggctgcagc tcgtgccaaa
     1321 ggcagagtag gtggacgccg tcctaagttg accaccgaac agtgggcaca gattggacgt
     1381 ttactcgagg ccggagaatc aagacagcgt attgcactga tttttgatgt gggcgtttct
     1441 accatttata gaaaatttcc ggcgaatgag agcaatgaat cgccctgagt cagtattatt
     1501 ttgatttttc ctgtaagcgg acaaataccg tcattttgtg tgaataacga cacaactgcg
     1561 cttagctgtt tgtcaggcac aatcacttca acatagggcg aagcctaatc caatcaggag
     1621 gttcgccact atggctcagg attaccacca cggggtgcgc gttgttgaag tcaacgaagg
     1681 cacccgatcc attaccacgg tgagcaccgc catcgtgggt atggtctgca cgggcgatga
     1741 tgccgatgca aaaatgtttc ctcttaataa acctgtgctg atcactgatg tgctgaccgc
     1801 cagcggtaaa gcgggtgagt ctggcacgct ggcccgttcg ctggatgcca tcgctgacca
     1861 ggcaaaaccc gtgaccgttg ttgtgcgtgt gccgcagggt gaaacggaag aagaaaccac
     1921 aaccaatatc attggcgcag tgaccgccga gggtaaaaaa acaggtatga aagccctgtt
     1981 atctgcccag tcacagctcg gcgttaaacc gcgcattctc ggcgtgccag gtcacgacac
     2041 gaaggcggta gctactgagt tgctgagcgt ggcgcaaagc ctgcgtggat ttgcttacct
     2101 gtcagcgtat ggttgcaaga cggtgcagga agcaatcact taccgtgaaa acttcagtca
     2161 gcgtgaagga atgctgatct ggcccgactt tactggctgg gacacggtgc tgaatgccga
     2221 agcaacggct tatgccaccg cccgtgcgct cggtctgcgc gctaaaattg atgagcagac
     2281 cggatggcac aaaagcctgt ccaacgtggg cgtgaacggt gtcaccggaa tttctgctga
     2341 tgtgttctgg gatctgcagg acccggcaac cgatgcaggt ctgcttaacc agaacgatat
     2401 caccacgctt atccgcaaag acggtttccg cttctggggt tcccgctgtc tgagcgatga
     2461 tccgctcttt gccttcgaaa actacacccg cacggcgcag gtgctgatgg acacgatggc
     2521 agaagcgcac atgtgggcgg tggacaaacc gctgaacccg tcgctggccc gcgacattat
     2581 cgaaggtatc cgcgccaaaa tgcgtagcct ggtcagtcag ggctatctca ttggtggtga
     2641 ttgctggctg gacgagtcgg tgaacgataa agacacgctg aaagccggga aactcaccat
     2701 cgattacgac tacacgccag tgccgccact tgaaaacctg atgctgcgtc agcgcatcac
     2761 cgatcagtac ctggtgaatt tctccagcca ggtcagcgcg taaggggaca acatggcttt
     2821 accacgcaaa ttaaaacacc tgaacctgtt taacgacggg aacaactggc aggggatcgt
     2881 tgagtcgctg acgctgccga aattcacccg caaatatgag aagtatcgcg gcggcggtat
     2941 gcccggagcg gtggatgtgg atctgggact ggatgacagt gcactggata cagaattttc
     3001 cattggtggt actgaactgc tgctgtttaa gcagatgggc aaagccacgg tggatggcat
     3061 ccagctgcgc tttaccggct ctattcagcg tgatgatacc ggggaagtgc aggccgtgga
     3121 gcttgtggtg cgtggacgtc acaaagaagt agattccggc gagtggaaga cgggcgaaag
     3181 caacactacc aaagtgacca gtaccaacag ctacgcgaag ctgaccatca atggtgaggt
     3241 gctctatgaa gtggacctga tcaacatggt ggaaattgtg gacggtgtgg acctgatgga
     3301 agcgcaccgc aacgccctcg gcctctgatg tatctgaacg gcgcggaatg ccgcgccaga
     3361 acctaattta caggacaaca aaatgagcga taagcagact gaaaagacca ttcaactgga
     3421 tactcccatc atgcgcggta aaacagaaat taccgaaatt gtgttgcgta aaccgcagtc
     3481 cggtgcgctg cgcggcacac gcctgcaggc cattatggat atggatgtga acgcgatgat
     3541 gaccgtgatc ccccgcatct ccagtccggc actgactgca caggaaattg cagagatgga
     3601 cccggcagat ctcactgcca tgtcggttga ggttgtcact tttttgttga agaagtcggt
     3661 gcttgccggt ttaccgacag cctgacggtt gacgatctgg tggctgatat cgccaccatc
     3721 tttcactggc cgccatccgt tactgacgtt atgccgctga ccgaagtgct ggaatggcgg
     3781 gataaagcga ttcagagaag cggggccagc catgagtgac aataacctgc gtctgcaggt
     3841 gattcttaat gcggttgaca aactcacccg cccatttcga tccgcgcagg ccagttcaag
     3901 agaactggct gctgctgtca aaaaatcccg cgatgcaata aagcagcttg atcaggccgg
     3961 gagcagtctg gacagcttcc gaaagctgca ggcagaaaat cagaaattag gcgacaggct
     4021 gaactatgcc cgccagcgtg caaatttgct cagtcaggaa ctgggagcga tggggccgcc
     4081 ttcgcaacgt caggttgttg ctctgggccg tcaacggctg gctgttcagc gcctagaaga
     4141 acgccagaaa aagctgcagc agcagacggc gcttgtgcgt gcagaacttt atcgtgctgg
     4201 tatttcagct aatgatggcg ccagtgcgac ggcccgcatt acccgtgaaa cgatgcgtta
     4261 taacaggcag ctttctgagc aggaagcgag gttacgacgt gtcggggagc aacagcgaaa
     4321 aatgcacgcc gcccgggggg cttacgccag gcgtcttgag gtaagggatc gtattgcagg
     4381 tgccggagcc accaccacgg ctgcagggct ggcaatgggc gcaccagtga tggcggcagt
     4441 aaaaagctat accagcatgg aagatgccat gaaaggtgtg gcaaagcagg tcaatggtct
     4501 gcgtgacgat aatggcaacc gcactgcgcg tttttacgaa atgcagggtg ccatcaaggc
     4561 tgccagcgaa cagttgccga tggaaaacgg tgctgtggac ttcgccgcac tggttgaagg
     4621 tggtgcgcgc atgaacgtcg caaaccctga cgacagctgg gaagaccaga aacgtgacct
     4681 gctggccttc gccagcacgg cagcaaaggc ggcaacagcc tttgaactgc cagcggatga
     4741 actgtcagaa agtctgggga aaatcgccca gctctacaaa atccctaccc gcaatattga
     4801 gcagctcggt gatgcgctga actatctgga tgataacgcc atgtcgaaag gggcggacat
     4861 cattgatgtc atgcaacgcc tgggcggtgt ggctgaccgt ctggattatc gtaaagcggc
     4921 ggcgctgggt tccaccttcc tgacactcgg cgctgcgcct gaggttgcag ccagtgcagc
     4981 aaacgcgatg gtgcgtgaat tgtccattgc caccatgcaa agcaagagtt tctttgaagg
     5041 gatgaatctg ctgaaactca atccagaagt gattgaaaag cagatgacga aggatgcgat
     5101 gggaactatc cagcgggtgc tggagaaggt gaatgcgctg ccacaggata agcgcctgtc
     5161 tgccatgacc atgttgtttg gtaaagagtt tggcgatgac gcagcgaaac tggcaaacaa
     5221 cctgccggaa cttcagcgcc agttaaaact gactgcgggc aatgatgcgc tcggttccat
     5281 gcagaaagaa tccgacatca acaaggattc actttctgct cagtggttgc tggtcaaaac
     5341 cggagcacag aacaccttca gcagcctggg ggaaacgctg cgccagccgc tgatggatat
     5401 tctgtacacg gtgaaaagcg tcacgggggc gttgcgtcgc tgggtggaag ctaacccgga
     5461 actgacgggc actctga
//
LOCUS       DADLTD010000227         5436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.231, whole genome shotgun sequence.
ACCESSION   DADLTD010000227 DADLTD010000000
VERSION     DADLTD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.231"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..913
                     /locus_tag="JN701_003634"
     CDS             <1..913
                     /locus_tag="JN701_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7365945.1"
                     /translation="PLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHG
                     VLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGE
                     VDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSV
                     SSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYAT
                     AGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKV
                     CTGRWRDLNLCVVVVAALFALKIILVD"
     gene            966..1418
                     /locus_tag="JN701_003635"
     CDS             966..1418
                     /locus_tag="JN701_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY7365946.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1629..2819
                     /gene="nepI"
                     /locus_tag="JN701_003636"
     CDS             1629..2819
                     /gene="nepI"
                     /locus_tag="JN701_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY7365947.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2860..3153)
                     /locus_tag="JN701_003637"
     CDS             complement(2860..3153)
                     /locus_tag="JN701_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7365948.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3375..4193
                     /gene="nlpA"
                     /locus_tag="JN701_003638"
     CDS             3375..4193
                     /gene="nlpA"
                     /locus_tag="JN701_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY7365949.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4220..5119)
                     /locus_tag="JN701_003639"
     CDS             complement(4220..5119)
                     /locus_tag="JN701_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7365950.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5230..5433)
                     /locus_tag="JN701_003640"
     CDS             complement(5230..5433)
                     /locus_tag="JN701_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAY7365951.1"
                     /translation="MLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYH
                     ALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1298 a         1238 c         1392 g         1508 t
ORIGIN      
        1 tcccttaagt ttacgtattg gtatcaccag cggaattgga ttatttatcg ccttaatggg
       61 attaaaaaat actggcgtta ttgtcgccaa taaagacacg ctggtgatga ttggcgattt
      121 aagttctcac ggcgtgttgt taggtatttt agggtttttt attataaccg tgttgtcatc
      181 acgtcatttt catgccgcgg tgctggtttc tatagtggtg acgtcttgct gtggattatt
      241 tttcggtgat gttcatttta gcggcgtcta ttccattccg cctgatatta gcggcgtcat
      301 tggtgaagta gatttgagcg gcgcgttaac acttgaactc gccggtatca ttttctcctt
      361 tatgctgatc aacctatttg attcatcagg aacattaatt ggtgtaactg ataaagcggg
      421 cttaatagat ggtaacggta aattccccaa tatgaataag gcgctgtatg ttgatagcgt
      481 cagttcggtg gcgggtgcgt ttatcggcac ctcgtctgtt actgcctata ttgaaagtac
      541 ttctggtgtg gcagtcggtg gccgcacggg actgactgcg gttgtggttg gcgttatgtt
      601 cctgttggtt atgttcttct caccgctggt ggcgatggtt cctccttacg caaccgccgg
      661 agcgttaatc tttgttggcg tgctgatgac ttcgagcctg gcgcgcgtta actgggatga
      721 ttttaccgaa tcggtgcctg cgtttattac cacggtgatg atgcccttta ctttctcgat
      781 caccgaaggg attgcactcg gctttatgtc gtactgcatc atgaaagtgt gcaccgggcg
      841 ctggcgcgat ctgaacctgt gtgtggtggt ggtcgcagct ctgtttgcac tgaagattat
      901 tctggtggat tagtaactgg cgagggtttc cagatatcat gagttctgat tacgcaggag
      961 aactcatgat ctggataatg ctcgccacgc tggcggtagt gtttgtggtt ggttttcggg
     1021 tgctgacatc cggggccaga aaagcgattc gccgtctcag tgatcggctg aatatcgatg
     1081 tcgtacccgt ggagtcgatg gtcgatcaaa tgggaaagtc agccggtgac gaatttttac
     1141 gttatttgca tcgcccggat gagtcgcacc tgcaaaacgc cgcgcaggtg ttgctcatct
     1201 ggcaaattgt cattgtcgat ggtagcgaac agaacctgct gcaatggcat cggattttac
     1261 aaaaagcccg tcttgccgcg ccgattaccg atgctcaagt caggctggcg ctgggttttc
     1321 tgcgcgaaac cgaacctgaa atgcaggata ttaatgcttt tcagatgcgc tataacgcgt
     1381 tctttcagcc tgccgagggc gttcactggc tgcattaaaa ccgcttcgtc ttatcagcct
     1441 acagaccgcg cctgacctgt cgggtggata agacgttcac gccgcatccg gtaatctttg
     1501 ccctgttccg tttcgatctt aactccagct caagcaacac cgtaaaagtt gcaaaaacgt
     1561 taaatgcgtc acacatttca atgttaaagt ggtcggcttt tcccctgaaa catgccacgg
     1621 gtaacaccat gagtgaattt attgccgaaa accgcggcgc ggatgccatc acccgaccga
     1681 actggtcagc cgttttctcg gtggcgtttt gtgtcgcctg tctgattatc gttgagtttt
     1741 tgcccgtcag tttgttgacg ccaatggccc aggatttagg catttcggaa ggggttgccg
     1801 ggcaatcggt gaccgtgacc gcctttgtgg caatgtttgc cagtttgttt attacccaga
     1861 caattcaggc tactgaccgc tgctacgttg ttattttgtt tgccgttttg ctgacgctct
     1921 cctgcttgct ggtttccttt gctaactcat tcagtttgct tttaatcggt cgtgcctgtc
     1981 tggggctggc gctgggcggg ttctgggcga tgtcggcgtc gctgaccatg cgtctggtgc
     2041 cgccgcgtac ggtgccgaag gcgctgtcgg tgatcttcgg cgcggtttct attgcgctgg
     2101 tgattgccgc gccgttgggc agttttttag gcgagcttat cggttggcgc aatgtcttta
     2161 atgcggcggc ggtgatgggc gtgctgtgta ttttctggat tatcaaatca ttgccttcac
     2221 tgccaggcga accctcgcat cagaaacaaa atactttccg cttattacaa cgtccgggtg
     2281 tgatggcagg gatgatcgcc atcttcatgt ctttcgccgg gcagtttgct ttcttcacgt
     2341 atattcgccc ggtgtatatg aacctggcgg gattcggcgt ggatggctta acgctggtgc
     2401 tgttgagttt tggtatcgcc agctttattg gtacgtcgct ttcgtcgttc attcttaaac
     2461 gttcggtaaa actggcctta gcaggcgcgc cgttaatact ggctgtgagt gcgttggtac
     2521 tgacgttgtg gggaagcgat aaaatcgttg ctaccggcgt ggcgattatc tgggggctaa
     2581 cttttgcatt ggttcccgtc ggctggtcaa cgtggatcac ccgctcgctg gccgatcagg
     2641 cagaaaaagc cgggtctatt caggtggcgg ttattcagct tgctaatacc tgtggcgcgg
     2701 caatcggcgg ttatgcgctg gataatattg gtctgacttc gccgttgatg ttgtccggca
     2761 cattgatgtt gctgactgca ttgttggtta ctgcaaaggt gaaaatgaag aaatcctgat
     2821 atcaggtaaa gaagcgttgg tcagtagttg ttgctgttct tacatatccg ggcattctac
     2881 cttacccaac gcttcccaat aattgctctg attattacgt tccatcgcca cgatggcctc
     2941 gcgtatatgt tgtttattgt tttcatctga caacattaac ttttcccacg cttcatctga
     3001 taatgacgcc tgtggcgtgc aaattttacg taaatcttgt aataccgtgg ttttctcttt
     3061 tgcggactgc agtgagctaa aaggcgactc ggcgtatacg atgcctggca tagcgaaaaa
     3121 agtgaaaata agcagtagtg tcgttggttt catggattca accttttaca aatgtgtatc
     3181 gaatgaagca taagtgtagt ttgctttcat cgctctcatc attctgtacg aaacgcgctt
     3241 gcgtggtcag taagaagtgc cagactttat attccacttt tattcctttt tattcttatc
     3301 gatagcgttt cgttttttaa accgcagcga ccttaccgct atagtcaggt aatcattaat
     3361 aaaaggataa aaaaatgaaa ctgacaacac atcatctacg gacaggggcc gcattattgc
     3421 tggccggaat tctgctggca ggttgcgacc agagtagcag cgatgcaaaa cacattaaag
     3481 ttggcgttat aaatggcgca gaacaagatg tcgcggaagt cgctaaaaag gtggcaaaag
     3541 agaaatatgg cctcgatgtt gaactggtag gatttagcgg ttcgttgcta ccgaacgatg
     3601 cgacaaatca tggcgaactt gatgcgaatg tcttccagca tcgacccttt cttgaacaag
     3661 ataatcaggc gcatggttat aaactggtag cggtgggaaa tacgtttgtc ttccctatgg
     3721 cgggttattc caaaaaaatc acaaccgtgg cgcaaataaa agaaggcgcg acagtggcga
     3781 tccccaacga cccgaccaac cttggtcgcg cacttttact gttgcaaaaa gagaagctaa
     3841 ttactctgaa agagggaaaa gggttattac ataccgcgct ggatataacc gataatcctc
     3901 gtcatttgca gattatggaa ctcgaggggg cgcagcttcc gcgcgtactg gatgatccca
     3961 aagttgatgt agcgattatc agcaccactt acattcagca gacagggctt tctccggtgc
     4021 acgacagcgt atttattgaa gataagaatt cgccgtatgt gaatattttg gtggcacggg
     4081 aagataataa gaatgcagaa aacgtgaagg aatttctgca atcttatcaa tcacccgaag
     4141 tcgctaaagc ggcagaaacc atttttaacg gtggcgcggt gcctggctgg taattctcac
     4201 tcatgccgcg ccggacgatt tatttttctg gccggcgacg ggaatccatt gaaatcaaaa
     4261 tcaccgatga cagaatcagc agcgttccca gccagtccgg taatgtgaac gtgatcccca
     4321 gcaacagcaa agagagtagc gcgctactta atggttctgc acagctcaaa atgctggctt
     4381 ttggaccgcc aattaattgt gctcctttca ggtacagact aaatgtcagg gacgtaccaa
     4441 tgaccaccaa ataaaaaaac gccagaatca aactgccgtt aacgacaaag tttgttcctt
     4501 gtctggcata aaaaggcaac agaatcagac cgccaatcag catactccag ccgacgactg
     4561 gtaacgtgcc atagcgggca attagcgttg agggataggt ggtatagaat gcagcagcaa
     4621 atgccgaggc aatgccccag aacaacgcgg caggagagag cgataatgac gtcggattac
     4681 cgtgtgtcac caataaaaaa gtcccgacca gcgatgtcaa aatagcgcag aaaaccagaa
     4741 tgcccgggcg cgatttacgc accagtgaga accaggcgac gataatcgtc ggtgagagga
     4801 attgcagcac cgttgccgtg gctgcgttcg atttttcgat ggttagcaaa aaagtgagct
     4861 gtacagttag cgcgccaacc acggaaaaaa tcagcaggct aatggcatct ttatgattgt
     4921 taataataga aaagatttta tcgccatgaa caaatgacag cgtcagtaga atcaaaccgg
     4981 cgaatatcaa acgcgtcata gtcaaaaact gcgacgacat ctggctttgc tccatgatgt
     5041 attgcgcgca gacccctgaa cttccccaca acacggcggc aatcagaacg ttcagcatcc
     5101 cctttctggt ggaacccatt tttccctctg catactgttt tgtcgtttat gtatgcaggc
     5161 atgatagcaa aaatggcgag gatggctctg tttgcgccac gccgattttt cttagcccag
     5221 actggtgagc taaatatctt taataaacag caggcaaatc atcgcaatac ccaacatccc
     5281 tatcgccagc cagaacaacg catggtagct ccagatttca accataattc cgtcaacaga
     5341 gccggcgata atccagccga cgcgtgaagt atttgcatat aacgttgtcg cagagcctat
     5401 tttttcaggc attaagtcct gaaaataaag catgcc
//
LOCUS       DADLTD010000228         5430 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLTD010000228 DADLTD010000000
VERSION     DADLTD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.232"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="JN701_003641"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="JN701_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY7365952.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="JN701_003642"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="JN701_003642"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY7365953.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHSTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="JN701_003643"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="JN701_003643"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY7365954.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="JN701_003644"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="JN701_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY7365955.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1343 a         1289 c         1475 g         1323 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc actcgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc
//
LOCUS       DADLTD010000229         5395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.233, whole genome shotgun sequence.
ACCESSION   DADLTD010000229 DADLTD010000000
VERSION     DADLTD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.233"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..562)
                     /locus_tag="JN701_003645"
     CDS             complement(<1..562)
                     /locus_tag="JN701_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7365956.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGG"
     gene            complement(781..1758)
                     /gene="epmA"
                     /locus_tag="JN701_003646"
     CDS             complement(781..1758)
                     /gene="epmA"
                     /locus_tag="JN701_003646"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7365957.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2083..3891
                     /gene="frdA"
                     /locus_tag="JN701_003647"
     CDS             2083..3891
                     /gene="frdA"
                     /locus_tag="JN701_003647"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7365958.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3884..4618
                     /locus_tag="JN701_003648"
     CDS             3884..4618
                     /locus_tag="JN701_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7365959.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4629..5024
                     /gene="frdC"
                     /locus_tag="JN701_003649"
     CDS             4629..5024
                     /gene="frdC"
                     /locus_tag="JN701_003649"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7365960.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5035..5394
                     /gene="frdD"
                     /locus_tag="JN701_003650"
     CDS             5035..5394
                     /gene="frdD"
                     /locus_tag="JN701_003650"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7365961.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
BASE COUNT         1382 a         1401 c         1385 g         1227 t
ORIGIN      
        1 gcccaccatt taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca
       61 ttactgcaat accgcccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa
      121 cgacggtgac cagaatcatc catgctactg ccagcagacc aaccacctgc gtaggctcca
      181 gtccggcaat acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta
      241 cccaaacttt cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg
      301 taccaatgaa ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccacctt
      361 cttctttgcg ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata
      421 acaatgcaga aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg
      481 ggctattggc aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca
      541 tcttttttat cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taacacgtgc
      601 ccactgaact gcactgcgca acattgagca ggcagcgtaa aaaccaatac agaattgata
      661 cccggatgat atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa atgaaaacat
      721 atgctacaga atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa
      781 ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa
      841 catcaccaga cgatcaacac ctaatgccac gccggaacag tcaggcatac cgactttcaa
      901 ggcttcaatc agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg
      961 gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc gttaattcat ggaaaccatt
     1021 cgccagctca atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt
     1081 actgatttgc gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc
     1141 tttgccaata tttggctcta cgccaaaggt aaacagcaat tgtagcaacg tgtcgcggtc
     1201 ttcttcggta tcagcaacgt tactcaaatc cagtttcgct gcgacttccc gcagttgcgt
     1261 tttgtcggca gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag
     1321 gctttctgct gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa
     1381 ccggtacata tcatagtgcg gtcgatacca ctcaagcata gtgaactcag ggttgtgata
     1441 acgccccatc tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccggc
     1501 aaccagcagg cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg
     1561 cgaatgcccg gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt
     1621 cgcctggctc atacaaggcg tctccacctc cagcactcca cgatcagcaa agaaacgacg
     1681 gatctccgcc ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca
     1741 ggatgccgtt tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg
     1801 caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca gtgactaaaa
     1861 aaagcacgat ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc
     1921 ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt
     1981 gtacctataa aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct
     2041 gaagtacggg gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc
     2101 gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag
     2161 gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc
     2221 gttgctgcag aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac
     2281 tttcacgata cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc
     2341 gtccaccact gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt
     2401 cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg
     2461 ttcgccgccg ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa
     2521 ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt
     2581 catgttcgcg gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct
     2641 aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc
     2701 ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac
     2761 atggaattcg ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa
     2821 ggctgccgtg gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat
     2881 tacggcatgg gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt
     2941 ccacgcgaca aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc
     3001 acgccgcgtg gcgatgtggt ttatctcgac ttgcgtcacc tcggcgagaa aaaactgcat
     3061 gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa
     3121 gaaccgattc cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag
     3181 aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg
     3241 cacggtgcaa accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtatg
     3301 gccggtgaac aagcgacaga gcgtgcagca actgccggta atggcaacga agaggcaatt
     3361 gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc
     3421 gaaaactggg cgaagatccg cgacgaaatg ggcctagcaa tggaagaagg ttgcggtatc
     3481 taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc
     3541 ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc
     3601 attgaactgg gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt
     3661 aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc
     3721 aacttcctca aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac
     3781 agcgacgtga agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat
     3841 gcagccgata aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa
     3901 cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt
     3961 ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga
     4021 caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg
     4081 cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac
     4141 cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga
     4201 tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg
     4261 caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt
     4321 ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc
     4381 agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga
     4441 ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac
     4501 tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca
     4561 gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg
     4621 agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga
     4681 aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt
     4741 ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gacgcctggg
     4801 cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg
     4861 cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca
     4921 ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg
     4981 tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt
     5041 aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt
     5101 atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg
     5161 gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc
     5221 attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt
     5281 atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac
     5341 ggtctggctg ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaac
//
LOCUS       DADLTD010000230         5386 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLTD010000230 DADLTD010000000
VERSION     DADLTD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.234"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            10..249
                     /locus_tag="JN701_003651"
                     /pseudo
     CDS             10..249
                     /locus_tag="JN701_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            705..1280
                     /gene="ssuE"
                     /locus_tag="JN701_003652"
     CDS             705..1280
                     /gene="ssuE"
                     /locus_tag="JN701_003652"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY7365962.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1273..2232
                     /locus_tag="JN701_003653"
     CDS             1273..2232
                     /locus_tag="JN701_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7365963.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2229..3374
                     /gene="ssuD"
                     /locus_tag="JN701_003654"
                     /pseudo
     CDS             2229..3374
                     /gene="ssuD"
                     /locus_tag="JN701_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            3439..4176
                     /gene="ssuC"
                     /locus_tag="JN701_003655"
                     /pseudo
     CDS             3439..4176
                     /gene="ssuC"
                     /locus_tag="JN701_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            4173..4924
                     /gene="ssuB"
                     /locus_tag="JN701_003656"
                     /pseudo
     CDS             4173..4924
                     /gene="ssuB"
                     /locus_tag="JN701_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(4967..>5386)
                     /locus_tag="JN701_003657"
     CDS             complement(4967..>5386)
                     /locus_tag="JN701_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HAY7365964.1"
                     /translation="MQEYDDKWHQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFT
                     MSNPNRIRSLIGAFAGSNPAAFHAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRL
                     KRYDAKRQEKMRAALEQLKGLENLSGDLYEKITKALA"
BASE COUNT         1237 a         1369 c         1452 g         1328 t
ORIGIN      
        1 gctgcatcgg tggtagcctg gttggcaaat gctgtaggtt gcacgccgtc agccgtacca
       61 ctaaaggtaa acgtcgcatt tttagttaca gtaacatcac agccttctaa ggcaattggt
      121 acatcagttt taacgccaga gactgctcca ttagtttgca acttggtggc gctgacattt
      181 ggcaatgtga ctactgaatc tttcgtcgca gctaccaggg tacaagctgg agcaataact
      241 ttaccattaa atgttacttt gccatccgtg atagcattat catcagctgc cataacgctg
      301 gacgttgcac ataccacagt tataaacgcc gtcaatacac ttttttcatc gtcatcatcc
      361 tgatctcttc gtaaatatga taatgccttt aaatgacatt agacttaata ataacaataa
      421 ttgaccgtca tttaaatcaa gcatactaaa tccaataagt gacaaatctt taatttatag
      481 atttcatgaa tatttaaata ttaactgaac gagtaattca ttgattcaac atctaatgac
      541 tatccaacaa ataaatatgc gtgatatata aagccctttc tttagtttat tttcagaaaa
      601 agatacacaa aatttatatt tggcattttc ttgtctctcc gacagactga ccgaattgtt
      661 atcaatgtta acaaaaaaag aacaattggt tataaggaga gagtatgcgt gtcatcaccc
      721 tggcgggtag tcctcgcttt ccttctcgct ccagctcctt gctggaatat gcgcgggaaa
      781 aactaaatgg cctggatgta gaggtttatc actggaatct gcaaaacttc gtcccggaag
      841 atctgcttta tgctcgtttc gatagtccgg cactcaagac cttcaccgaa cagctgcaac
      901 aggctgatgg actgatagtc gctacacctg tgtataaagc cgcctattcc ggcgcgctga
      961 aaaccctgct cgacctgctg ccagagcgcg ctttgcaagg caaagtggtg ctaccgctgg
     1021 cgacgggcgg taccgtggcc catctgctgg cggtcgatta tgcccttaaa ccagttttaa
     1081 gcgcactgaa agctcaggag atcctgcacg gcgtgtttgc cgatgactca caagtaattg
     1141 attaccatca cagaccccag ttcacgccaa atctgcaaac ccgtcttgat accgcgctag
     1201 aaactttctg gcaggcattg caccgccgcg atgttcaggt tcctgacctt ctgtctctgc
     1261 gaggtaatgc ccatgcgtaa catcattaaa ctggcgctgg tgggattgct tagcgtctct
     1321 acgattgcgg ttgctgcaga atcctcgcct gaagcgttat gtataggcta tcagaaaggc
     1381 agtattggta tggtactggc aaaaagccac cagttactgg aaaaacgcta tccgcaatca
     1441 aaaatctcct gggtggagtt ccccgcgggt ccgcaaatgt tggaagcctt aaacgttggc
     1501 agtattgatc tcggcagtac cggggatatt ccgccaatct ttgcccaggc tgccggggct
     1561 gatttggtat atgtgggcgt cgagccaccg aagcccaaag ccgaagtgat tctggtggca
     1621 gaaaacagcc cgatcaaaac cgtagccgat cttaaaggtc acaaagttgc ctttcagaaa
     1681 ggttccagtt cacacaacct tttactgcgt gcactgcgtc aggccggact taagtttacc
     1741 gatatccaac ccacttacct gacgcccgct gatgcccgcg ccgcgttcca gcaaggtaac
     1801 gttgacgcct gggctatctg ggatccctac tactctgctg cattattaca gggcggcgtg
     1861 cgggtgctga aagacggcac cgatctcaat caaactggat cgttttatct ggcagctcgt
     1921 ccctatgcag aaaaaaacgg cgcttttatt cagggcgtac tggcaacctt tagtgaggcc
     1981 gatgcgttaa cccgcagcca gcgcgagcaa agcatcgctt tactggcaaa aacgatgggc
     2041 ttaccggcac cggtgattgc ctcttactta gatcatcgcc ctcctaccac catcaaaccg
     2101 gttaacgccg aggttgccgc cttacagcag caaacggcag atctgtttta tgaaaatcgt
     2161 ctggtgccga aaaaagtcga tattcgccag cgcatctggc agcccactca actggaagga
     2221 aaacaattat gagtctgaat atgttctggt ttttaccgac ccacggtgac gggcactatc
     2281 tgggaacgga agaaggttca cgcccggttg atcacggtta tctgcaacaa attgcgcaag
     2341 cggcggatcg tctgggctat accggtgtgc tgatcccaac ggggcgatcg tgtgaagatg
     2401 cgtggctggt ggcggcgtcg atgatcccgg tgacgcagcg gctgaagttt cttgtcgccc
     2461 tgcgccccag cgtaacctca cctaccgttg ccgctcgcca ggccgccacg cttgaccgtc
     2521 tctcaaatgg tcgtgcgttg tttaatctgg tcacaggcag cgatccacaa gagctggcag
     2581 gcgacggcgt gttccttgat catagcgagc gctacgaagc ctcggcggaa tttacctagg
     2641 tctggcggcg cttattgctg ggggaaaccg tcaatttcaa cggtaaacat attcatgtgc
     2701 gcggagcaaa actgctcttc ccgccgattc aacagcctta tcctccgctt tactttggcg
     2761 gatcgtcaga cgtcgcccag gagctggcgg cagaacaagt tgatctctac ctcacctggg
     2821 gcgaaccgcc agaactagta aaagagaaga tcgaacaagt gcgggcgaaa gctgccgcgc
     2881 atggacgcaa aattcgtttc ggtattcgtc tgcatgtgat tgttcgtgaa actaacgacg
     2941 aagcgtggca ggccgccgag cggttaatct cgcatcttga tgatgaaact atcgccaaag
     3001 cacaggccgc attcgcccgg acggattccg taggacaaca gcgaatggcg gcgttacata
     3061 acggcaagcg cgacaatctg gagatcagcc ccaatttatg ggcgggcgtt ggcttagtgc
     3121 gcggcggtgc cggaacggcg ctggtgggcg atggtcctac ggtcgctgcg cgaatcaacg
     3181 aatacgccgc gcttggcatc gacagctttg tgctttcggg ctatccgcat ctggaagaag
     3241 cgtatcgggt cggcgagttg ctgttcccac atctggatgt cgccattccg gaaattcccc
     3301 agccgcagcc gctgaatccg caaggcgaag cggtggcgaa tgattttatc ccccgtaaag
     3361 tcgcgcaaag ctaaggaaga aataatggca acgccagtga agaagtggtt attgcgcgtt
     3421 gccccctagt ttttaccggt gggcatcgtg gcggtgtggc aactggcctc ctcagttggc
     3481 tggctgtcga cgcgtatttt gccctcaccg gaaggggtgg tgacggcgtt ctggacgctc
     3541 tccgccagcg gcgaactgtg gcagcatctg gcgatcagtt cctagcgggc gctgattggc
     3601 ttttcaattg gcggatcgct ggggttgatt ttgggactga ttagcgggct atctcgctgg
     3661 ggagaacggc tgctggatac ctcaattcag atgttgcgca acgtgccgca tctggcgctg
     3721 atcccgctgg tgattttgtg gtttggcatt gatgaatccg caaaaatctt tctggtggcg
     3781 ctcggtacgc tgtttcccat ttatatcaac acctggcatg gcatccgtaa tatcgatcgc
     3841 gggctggtgg agatggcgcg tagctatggg ttatccggca taccgctgtt tatccatgtg
     3901 atcctgcctg gtgccctgcc ctcaattatg gtcggcgtgc gttttgcgtt gggcctgatg
     3961 tggctgacgc tgattgttgc cgaaaccatt tctgccaatt ccggcattgg ttatctggcg
     4021 atgaatgcgc gagagttttt gcaaacggac gtggtggtag tcgctattat tctttacgcc
     4081 ctgctcggca aacttgctga cgtcagcgcg cagctgttag agcgcctctg gttgcgctgg
     4141 aacccggctt atcatttgaa ggaggccacg gtatgaatac tgctcgtctg aaccagggca
     4201 caccattgtt gctcaatgca gtaagcaaac attacgcgga aaatatcgtc ctgaaccaac
     4261 tggatttaca tattccggca ggtcagtttg tggcggtggt ggcaaaagta ccctgctgcg
     4321 cctgctggcc gggctggaaa cgccaaccgc aggcgatgtg ttggcaggca ccacaccgct
     4381 ggctgaaatt caggaagaca cgcgaatgat gtttcaggat gcgcgtctgc taccgtggaa
     4441 atcggtgatt gataacgttg ggttaggcct taaaggccag tggcgcgatg ccgcgcgtcg
     4501 ggcgttggct gcggtaggac tggagaatcg cgccggggaa tggcctgccg cactttcagg
     4561 cgggcagaaa cagcgagtgg tgctggcaag agcgttgatt catcgaccgg gtttattgtt
     4621 gcttgatgaa ccgctcgggg cactggacgc cttaacgcga ctcgagatgc aggatttgat
     4681 tgtgtcactt tggcaggagc acggctttac cgtactgttg gtgacgcatg atgtgagcga
     4741 agcggtagca atggctgacc gggtgttatt aattgaagag ggaaaaattg gtctggattt
     4801 gacagtggat atcccccctc cgcgtcgatt agggtcggtg cgattagcgg agctggaatc
     4861 ggaagtgttg caaagggtga tgcagcgtgg tgaatctgaa attcgcctga gaaaacaggg
     4921 gtaaattccc cgaatggcgg cgctattgcc gccattcggt tatttatcaa gccagtgctt
     4981 tggtgatctt ctcgtacaga tcgccagaga gattttccag ccctttcaac tgttccagcg
     5041 ccgcgcgcat tttctcctga cgtttggcat cataacgttt caggcgaatc agcggttcaa
     5101 tcagacgtga agccacctgc gggttacggc tgttgaggtc ggtaagcatt tccaccagga
     5161 actggtaacc gctcccatct tcggcatgga acgctgccgg attgctgccc gcaaacgcgc
     5221 caatcaacga acgaatacgg ttcgggttgc tcatggtaaa tgagcgatgc tgcaacagac
     5281 cgcgcaccgt ctccagcaca ttcgccgccg ggctggtggc ttgcaggata aaccatttat
     5341 ccatcaccag accgtcctga tgccacttat cgtcgtattc ctgcat
//
LOCUS       DADLTD010000231         5339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLTD010000231 DADLTD010000000
VERSION     DADLTD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.235"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(892..1833)
                     /gene="fabY"
                     /locus_tag="JN701_003658"
                     /gene_synonym="yiiD"
     CDS             complement(892..1833)
                     /gene="fabY"
                     /locus_tag="JN701_003658"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY7365965.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1878..2315)
                     /gene="dtd"
                     /locus_tag="JN701_003659"
     CDS             complement(1878..2315)
                     /gene="dtd"
                     /locus_tag="JN701_003659"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY7365966.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2312..3185)
                     /locus_tag="JN701_003660"
                     /pseudo
     CDS             complement(2312..3185)
                     /locus_tag="JN701_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3179..3778)
                     /gene="yihX"
                     /locus_tag="JN701_003661"
     CDS             complement(3179..3778)
                     /gene="yihX"
                     /locus_tag="JN701_003661"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY7365967.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3919..4806)
                     /locus_tag="JN701_003662"
     CDS             complement(3919..4806)
                     /locus_tag="JN701_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7365968.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLQENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4796..>5339)
                     /locus_tag="JN701_003663"
     CDS             complement(4796..>5339)
                     /locus_tag="JN701_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7365969.1"
                     /translation="GIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQK
                     SLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGT
                     KVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIE
                     PTDEQETASYRYIREQSHEN"
BASE COUNT         1439 a         1346 c         1254 g         1300 t
ORIGIN      
        1 tctgataaat gaagaaaaac cgtattcatc gccattacca ccaccttacc tatattactc
       61 attaatgtat gtgcgaaaat tacgccggaa aatcattaaa aaaaagcgga tttttgcatg
      121 ttgttttgtt tttgcgaggc gttttccaga aataataaga gtgttgcagt tgtttcattt
      181 attactcaaa aatggtttag ttatcgccca ggatgaatta tgggtgctat gaatatatta
      241 tattcagaga tatttatttg cataatctga atggtgtagc cgaaaaacgt cagcatccta
      301 acagcaccag gatttaaggt gaaattaatc tttcatcaca gaccggtcag tactgccacc
      361 aaccggtctt tacttgctca tcgttataat tttgttggta acagttcggg aaagttgttt
      421 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
      481 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
      541 agtaaatgaa tgatactgac aatgatgcca gcccattgtc tttgcataat gtttgtgagc
      601 tatagatttt aaaaatattt atgaggaaag tatggcacca gaggaggttt tgactatcaa
      661 aaatcgctca aacctgaact aagatgatga gttaagcatt atctgcgata attgccactc
      721 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      781 caacgaagaa taaaaattta tagagttctt attcaatatt accggcatag acagggaatg
      841 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      901 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      961 ttcaaacact gcacccggcg tctcgtcgcc aaagatttca acttgcatct gcacccgtgc
     1021 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
     1081 atgaggttta ccgctaatcg gcttgctgta gcggatatgc gcatcggcca gaataatcgt
     1141 tccgccgagg tggcgttcgc gcagcatcag ccagataagc ccccaaccgg tgagcgttgc
     1201 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattac cggtttccgg
     1261 catggtggtg ataaattttt gcccggtata ttgctgaatg cgcacgccca ttttttcact
     1321 aagcgggatg tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1381 aatatcatcc agagtggcaa caggtttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1441 tggtgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1501 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1561 catcgccatc aacgtcccca gccctttgtc ctgcacgtcg ggatgaacgg ccataaagcg
     1621 aatggacgct tcattgtcgg cattaatata cagtcggcct accgccacca gattcccctg
     1681 ctcgtcgacg accatctgat gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1741 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1801 ttcttctgtt tgtggaaccc gaaggtgata catagcgata ctctctcttg tttcccgtga
     1861 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1921 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1981 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     2041 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     2101 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     2161 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     2221 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     2281 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2341 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2401 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2461 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2521 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2581 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2641 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2701 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2761 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2821 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2881 tcatcttgtt ggaattggca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2941 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     3001 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgacc
     3061 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     3121 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     3181 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     3241 taatgcccag ctgattggct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3301 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     3361 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     3421 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     3481 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     3541 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     3601 cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     3661 tatgaaaact cttcttaagc gtagccagtg gaacacgcgt taaatcgctc caggctccca
     3721 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3781 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3841 ggcaataagg gtaatcttgt gatgctgatt gcgaaaaagc agagtaattt cactggcttt
     3901 ttcgcaatag atgaatggtt aagattcagg tgtaaattgc caacgagttt cgatgaccag
     3961 cggcctatct ggtgtcacca attccggcgt tagatttatt gcgttaggtg cctgagtcag
     4021 cgggttaata caggtggcat cgggctgttt atcaaatacc accagactgt ttgcagaaga
     4081 gatcatggtc atcgcgagtt taccaggcca cagtaactta accttcaccc cgttataaaa
     4141 accaaaacag tcatcccagg gaccggcttg aggagagata cgatttccag ttggcagttc
     4201 atcactaccc gcttcttctt gccagtccgc atcaaaaagc acctgcaacg attctgtatt
     4261 ttgcggagtc agctttttcg caaaccacgg atgccagcca gcggaggccg ggaatgtatc
     4321 ggcatatgaa tggacttcca gttgcaaaac cagcgcatcg ttctcctgca aaaaagtctg
     4381 aatcacttcc ccttgccagg gccacggtga agccaggggc atacgtaaag tcagggaatc
     4441 tgctgttttg tcgatgattt cccacgttga gtaacaagcc ataccatgca acgcatgtgg
     4501 tggtttattg gcgggtaaag aataacattg cccaccagca ttcagcgtcg cattccccag
     4561 tcgccctgcc cacggcacca ttgggaaaca gccatactga aaagctctgc gctgcggctg
     4621 ccattggcgt aatacttcag aaccaaatgc ctttaacgag gttatcctgg caccatcatg
     4681 cggataaatc tgcaggtgaa tattgccttt ttccagcgtc actaactctc ctacaggccc
     4741 aggccatggc gcgtcaaacg cctgttgtag cgccaggtaa tgctcgtttt ttcgttcagt
     4801 tttcatgaga ttgttccctg atgtagcggt aagaagcggt ttcttgttca tcggtcggct
     4861 caatcagcca gacgcaaaca aaggaaagaa ctgagacagc aaataaaaac agagcgacat
     4921 accacggttt tcctccccct agcgccagca aacttgaggc gataaggggg cttagtccgc
     4981 caagaatcgc ggaaaattga taagcaaaag aaaggccggt atatcgtact ttggtaccga
     5041 acatgcgggt gaaaagtgtt ggctgcacgg caaacatcat cgtcggacct aagttgtagc
     5101 cgaggatcat actgcaccag atgatgagcg tacttttgct atccagtaat aagaagaaag
     5161 gaaacgcgaa caggacacaa aatcctgctc ccgacaaata cagacttttt tgacctatac
     5221 gatctgataa ccatccaacc aatggacaac tgaaaatacc gatcgccgag gcaatcaaca
     5281 tacccgtcag gggaatatct cgcgacaata ctaattggct ggaaatatag acaatacca
//
LOCUS       DADLTD010000232         5277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLTD010000232 DADLTD010000000
VERSION     DADLTD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.236"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..421
                     /gene="speG"
                     /locus_tag="JN701_003664"
     CDS             <1..421
                     /gene="speG"
                     /locus_tag="JN701_003664"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HAY7365970.1"
                     /translation="TTSHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIIS
                     PEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHE
                     FFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(424..1134)
                     /locus_tag="JN701_003665"
     CDS             complement(424..1134)
                     /locus_tag="JN701_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY7365971.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            1242..1547
                     /locus_tag="JN701_003666"
     CDS             1242..1547
                     /locus_tag="JN701_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY7365972.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            1746..3164
                     /locus_tag="JN701_003667"
                     /pseudo
     CDS             1746..3164
                     /locus_tag="JN701_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            3195..5064
                     /locus_tag="JN701_003668"
                     /pseudo
     CDS             3195..5064
                     /locus_tag="JN701_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            5075..>5277
                     /locus_tag="JN701_003669"
     CDS             5075..>5277
                     /locus_tag="JN701_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY7365973.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELGCKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISC"
BASE COUNT         1412 a         1323 c         1273 g         1269 t
ORIGIN      
        1 gaccacttca catattcacg atcagagcga acgacgcttt gtggtggaat gtgacggcga
       61 aaaagccggt ctggtggagc tggtggaaat taaccatgta catcgccggg cagaatttca
      121 gataattatc tccccggagt atcaggggaa aggtctggca acccgtgccg ccaaattagc
      181 aatggactat ggcttcaccg ttctcaatct ctataagctg tatctgatcg tcgataaaga
      241 gaatgaaaaa gcgattcaca tttaccgcaa gcttggcttt acggttgaag gtgaattgat
      301 gcatgagttt tttattaatg gtcaatatcg taatgccatt cgcatgtgta tattccagca
      361 tcagtatctg gcagagcaca aaacaccggg tcagactctc ctgaagccga ccgcacaata
      421 gcattaataa taatcgatcg tatttttgat ggtgtaaacc cgttcgacgg cgggttttac
      481 tccttcatca acaatgatca gctgacagtc caccggattg gcgtgactgt catattcaca
      541 gctctgtttt acattaccaa ctcgttgatt attcagtaaa gtaaccgctg tgtaatctaa
      601 ttttttgatc gggtccgttg atggcgtggc gctgacagat aatgttttgt cgttactttt
      661 cgtggtttta cccagtggat aaccctgatc atcatagcga tattccatct gcatttgttt
      721 gctgctggct ttaatcacga agccattatc atcggtttcc caactcaccc cggcagaagg
      781 taattctgct agctggcatt ttccctgtaa acgtactctc ttctccagcg tctcggcatc
      841 acggtaataa ttggcgtcca gtaccagagc gaccacggta ttattttcca gatccagtaa
      901 ttcgagtgaa tcaaaacagc cttcttccga caaagtccca gaaacacgtt tcgtcacttc
      961 accttgctca tccattaatg tctgagtgaa atcttttacc ggaccacgca gcggatcaaa
     1021 atcgaattca ttagagaaac tggccatctc aggggtaaat gaaagtgtta cctctgtgcg
     1081 gtcacatcct gtgaggaata tcgcgagtaa gcatggtagt aatttgtatt tcacaacagt
     1141 caccagagag tagagatgat tctcaatcat agtagcaaat acagtacttt acacgttaaa
     1201 tgctatgctt aaagaagtta tcttcgcgta aggagcttat gatgaaactc tcaacctgct
     1261 gtgccgcgct tctgctcgcc ctcgcctcac ccgcggtact cgccgcgccg ggatcctgtg
     1321 agcgcataca gagcgatata tcacagcgca ttatcaataa tggcgtaccc gaaagcagct
     1381 tcacattaag tattgtaccc aatgaccagg ttgatcagcc tgattcccag gtcgtcgggc
     1441 attgtgctaa tgatacgcat aaaattctct acacccgcac caccagcgct aacgtctctg
     1501 ctcccgcgca gtctacccag gatggcgcgc ctgccgaacc gcagtaatac attatcgact
     1561 gaacgccgga tatgacacat ccggcgattt gaacatacaa cataatccca ccttattact
     1621 catacccttc tattgatgtg gattaataat tcttaaccca aaatgggtag accccctcta
     1681 ttgttagcgc gctaaatatt caatacataa acatttatat aacgataaag aacagggagt
     1741 gagttatgtc caaaaatgac cgaatggtgg gcatcagccg cagaacactc gttaaatcaa
     1801 ccgcgatagg ttctctggcg ctggctgcag gcggtttttc tttaccgttc accctgcgca
     1861 gtgcagcggc aacggtacaa caggccagcg aaaaagtgat ctggggtgcc tgttccgtca
     1921 actgtggtag ccgctgtgca cttcgtctac atgttaaaga taatgaagtg acctgggtgg
     1981 aaactgacaa taccggcagc gatgagtacg gcaaccatca ggtacgcgcc tgtttgcgcg
     2041 gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaattaccca atgaaacgcg
     2101 tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc ctggatacta
     2161 tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaggctgta tatattcagt
     2221 actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc tcggcggtca
     2281 aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac agcaccgcac
     2341 aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc accacggata
     2401 ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc cgcatgagcg
     2461 gcggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc aaaatgattg
     2521 ttatcgatcc gcgatatacc gatacggctg ctggtcgtga agatgaatgg cttcctattc
     2581 gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt aacgaaaatc
     2641 tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa accttacccg
     2701 cagatgcacc aaaaaatggt cactataaag cctatattct tggtgaaggt gatgataata
     2761 cagcgaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtggac cgtatcatca
     2821 aactggcgcg tgaaattggc acagcaaaac ccgcttatat ctgccagggc tgggggccgc
     2881 aacgccaggc aaacggcgaa ctgactgcac gcgctattgc tatgttgcct attttgacgg
     2941 gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat accattacca
     3001 tagaacgcct gccggtgctg gataatccgg tcaaaacgtc aatctcctgc ttcagctgga
     3061 cagatgctat cgatcatggt ccgcaaatga cggcaatccg cgacggcgtc cgcggcaaag
     3121 ataaactgga tgtgcccatt aagttcatct ggaactacgc gggaaagatg aaaatccata
     3181 ccacagaggc gctcatgaag gctgaaatca gccgtagaag tctgatgaaa acctccgcac
     3241 ttggcagtct tgcgctggca agcagtgctt tcactctgcc attttcccaa atggtccggg
     3301 cggcagaggc tccggtagaa gagaaagcgg tctggagttc ctgcaccgtt aactgcggga
     3361 gccgctgtct gttacgtttg catgtgaaag atgacaccgg caagcccgcg aacgttcaaa
     3421 cgcacgcatg atcgtcatcg atccacgtta taacgacact gctgccgggc gtgaagatga
     3481 atggctgcct attcgccctg gcaccgatgg cgcactggcc tgtgcgattg cctgggtact
     3541 gattactgaa aacatggtcg atcagccatt tctcgacaaa tattgtgttg gttacgatga
     3601 aaaaacgctg cccgccataa agcctatatt ctgggcgaag ggcctgacgg catagctaaa
     3661 acgccggaat gggcagcaaa aatcaccagc atcccggcag aaaaaattat ccagttggcg
     3721 cgagagatcg gttcagcaaa acctgcttat atttgtcagg gttgggggcc acaacgacat
     3781 tccaacggcg agcaaacgtc ccgcgctatt gccatgcttt ccgttctcac cggtaacgtt
     3841 ggcataaacg gcggcaactc aggtgtacgc gaaggtagct gggatctggg ggtagaatgg
     3901 ttcccgatgc ttgagaatcc tgttaaaacg cagatttccg tctttacatg gacagatgcc
     3961 atcgaccatg gtacggaaat gaccgcgacc cgtgatggtg ttcgtggaaa agaaaaactg
     4021 gatgtcccca tcaagttttt atggtgctac gccagtaaca cattgatcaa tcaacatggc
     4081 gacatcaatc acacccatga ggtgcttcag gatgacagca agtgcgagat gattgttggc
     4141 attgaccact tcatgacggc ctcggctaag tattgcgata tcctgttgcc cgacctgatg
     4201 ccaacagagc aagaagacct tatctctcat gaatctgcag ggaatatggg ctatgtgatc
     4261 ctcgcccaac ccgcaacctc agcaaaattt gaacgcaaac ccatctactg gatgctgagt
     4321 gaagtcgcca aacgcttagg accagacgtt tatcaaacct ttactgaagg tcgcagtcag
     4381 catgaatgga tcaaatatct ccatgcgaaa acgaaggaac gtaaccctga gatgctcgac
     4441 tacgaggaga tgaaaacgac cgggatcttt aagaaaaaat gcccggaaga acactacgtc
     4501 gctttccgcg cattccgtga agatccacag gcaaacccgt tgaaaacacc ttcggggaaa
     4561 atcgaaattt attctgaacg actggcgaag attgcagata cctgggaatt gaaaaaagat
     4621 gaaattattc atccccttcc tgcgtatacc ccaggttttg atggctggga cgatcccctg
     4681 cggaaaacct atccactgca gttaacgggc ttccactaca aagcgcgtac ccactccagc
     4741 tacggcaata ttgatgtgtt acagcaggcc tgcccacaag aggtgtggat caaccccatt
     4801 gatgctcagg cacgcggtat ccgtcatggc gataccgtgc gggtatttaa caataatgga
     4861 gaaatgctga ttgccgcaaa agtgactccg cgtattctgc ctggcgttac cgccatcggg
     4921 caaggtgcgt ggcttaaagc ggatatgttt ggtgaccggg tcgatcacgg cggcagtatc
     4981 aatattctga cctctcaccg cccttcaccg ctggcaaagg gaaacccgtc gcacagcaat
     5041 cttgtccaga tcgaaaaggt ttaaggagta gcccatgacc acacaatatg gattttttat
     5101 cgattccagc cgctgtactg gctgtaaaac ctgcgaactg gggtgcaaag attttaaaga
     5161 tcttggcccg gaagtcagtt tccgccgtat ttatgaatat gctggcggcg actggcagga
     5221 agataatggc atctggcacc agaacgtgtt cgcttattat ctctccatct cctgtaa
//
LOCUS       DADLTD010000233         5208 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLTD010000233 DADLTD010000000
VERSION     DADLTD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.237"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..117
                     /locus_tag="JN701_003670"
     CDS             <1..117
                     /locus_tag="JN701_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HAY7365974.1"
                     /translation="DWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(167..1915)
                     /locus_tag="JN701_003671"
     CDS             complement(167..1915)
                     /locus_tag="JN701_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY7365975.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1915..2985)
                     /locus_tag="JN701_003672"
     CDS             complement(1915..2985)
                     /locus_tag="JN701_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY7365976.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDAVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2975..4426)
                     /locus_tag="JN701_003673"
     CDS             complement(2975..4426)
                     /locus_tag="JN701_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HAY7365977.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4437..4883)
                     /locus_tag="JN701_003674"
     CDS             complement(4437..4883)
                     /locus_tag="JN701_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY7365978.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1317 a         1343 c         1317 g         1231 t
ORIGIN      
        1 gactgggata aagagcaact tcaggacgca aactcaagct ggatggtcga agactctttc
       61 ccgagagcat tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaacg
      121 tttttgatga taaatgcagt gtcggatgcg gcgcatctga cacagttcac cccttgtgaa
      181 gtaactccat aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac
      241 acaatatgct tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc
      301 aggcgatcaa gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa
      361 taaaaaatcc acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat
      421 taacaatcag gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg
      481 taatatggtg ttgtgcgact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt
      541 caccgcgtac attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggttgct
      601 ggcgaataaa tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga
      661 tattttttac ggtgatgtaa ttatttaccg cgtcttccag taaataatgg ctattgttaa
      721 tgatcaataa cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacgggcaa
      781 taattacccg acaatttaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg
      841 cgttctggtc cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgc tccagcgcac
      901 aggcaaaata caaaccgatc agatcgctgt cgaaaagggg aatatcgagc tgttcacgca
      961 gtagcgtaat aaagtgcaga ctcatatcaa aggccgcagg ccaggctgct ttcaggttat
     1021 tcatgctact ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat
     1081 gcccggtaat attctcaacc aactgcgtat caataatatt caatgacgcg atgtctggca
     1141 ccctttgcag caaccccagc acatgatcgg ctgaaattac ctgcgcttgc tgatgctgtt
     1201 tctctatcaa acctgtcagg gttcttacag cgttatcacc aagaaacatg ccgctttgtt
     1261 caacgatttg ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac
     1321 gcagggcgta aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc
     1381 ggtgttggtt gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca
     1441 gcggaattaa aaacggatct ttgcgcagca agttcgccag caagataacg cgcttctctt
     1501 ctgtctcatc aatgaaatgg cccaaaccgg ggcggctggc caggcaacaa gcgcgttcat
     1561 aacgctgttt taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg
     1621 aggcgagttg cgcacgggga gtaaaggtat tcagtaataa cagtgccagc agccgatagt
     1681 cgttatcgtg cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat
     1741 agcccgcact accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt
     1801 caatatcacg caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg
     1861 tgcgcggctg ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc
     1921 catctgacgg aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc
     1981 ccgtatcaac gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg
     2041 cagatagcgg gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta
     2101 gcgcccgcca tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg
     2161 tgcggcacag ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa
     2221 cagcatcagc cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg
     2281 aacatcgcct gcgacagcgg tatccagcac gatcactgca tccggtttaa tgtgttccgc
     2341 cgaggtttgt gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt
     2401 aatttcggga ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt
     2461 atccagcgcc ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct
     2521 aataaaatcg cccatcgcca caccgcgttt ttccacctca tcgcgactgt ttgcgccaat
     2581 atcaatgaac atctcatcaa atgacagcgg ccgttgcctt tgcttttccg ttaacgcatg
     2641 aggcgcgacg gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac
     2701 ccggtggttg agcatcgact gattccacca gccaccaatg gtggtgaaac gcagaaaacc
     2761 gctctcgtcg atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac
     2821 ttttggacct ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatctc
     2881 attaacgcaa ggttccagcg tgtttatcag aatgtcgcga acttcctgtt catcgccgct
     2941 gacggcgctg gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt
     3001 gcgcggcttt cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt
     3061 tcagcgcgag gccaatcgcc agcaccagat aaaacgaacc caccggcgac atcaggccga
     3121 tcagcgggtc aaagataccc aaaccaggtg ccagacgttt gattccgaaa gcgatcacca
     3181 gcacgccggt aatgccaccc gccagcgtat tggcggtaat catcggcaac ggagctgcca
     3241 gagcgtaagg tatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg
     3301 cagcctcttt ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg
     3361 caacgggcgg catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt
     3421 ccagcagtga gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg
     3481 ccagcatcgc accgaccaga aacgctgctg caaacttcat tgatggagga atggtattca
     3541 ggaagtggag cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga
     3601 gcacgccgaa aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta
     3661 atgctttgcc aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca
     3721 gcaccaccgc gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg
     3781 ccagatagca aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa
     3841 tataagcgcc catgatgggg atcataaagg tgaagccaag gtaaccgatg gattcaacta
     3901 cccaggtaaa cgacggcgcg cctttcgaca tatcggtata cggcaaaccg aactgcacca
     3961 gcatgttggc aagcgccacc aggataccac cgccgatgac aaacggcagc gccgcagaga
     4021 cgcccgccat caggtggctc atcacgctgc cgctttgcat ttcctgtttg ccaagcttca
     4081 cgccactatc tgcggcaaaa agctgcgagt ttgtcggtaa ttcgctgaaa atctggtcga
     4141 tatttttcaa cgcctgggag atggcaatct cataaacttt cttcccggta aaccgcgcgc
     4201 gatcatcacc gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct
     4261 cttcgctgga taagcggttt tcaacgccac tggacccttg agtttccact tttatggtat
     4321 ggccgagaga acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc
     4381 cggcggggca gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat
     4441 gagctgaggg tttgatttaa caaggcaagc acttggtcgg tatcgccctg ttgcagttga
     4501 tgaataaatt ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc
     4561 tcttcgccgc tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg
     4621 gctttccagt caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag
     4681 gcgcttttac cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg
     4741 cgcagcagca gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct
     4801 attgtcgcca actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata
     4861 cagcttgcag taagagctgc cattaatcgg actccataaa atatatttta atcaattgta
     4921 atggtggagc gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca
     4981 gagtgaaagg tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga
     5041 atcattaatg ggttattcat tatttttgtg atttactcac aatgtatcag ggaaatataa
     5101 cttaccggga gatgtaatat gtttaatttt tcaaatcgaa atttaaaata ttgtgccgga
     5161 ggcatctccg gcacattggg caattacggc aggtaaaaca cttcttgc
//
LOCUS       DADLTD010000234         5190 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLTD010000234 DADLTD010000000
VERSION     DADLTD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.238"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..148
                     /locus_tag="JN701_003675"
     CDS             <1..148
                     /locus_tag="JN701_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY7365979.1"
                     /translation="FSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVV
                     VIGR"
     gene            209..3688
                     /locus_tag="JN701_003676"
                     /pseudo
     CDS             209..3688
                     /locus_tag="JN701_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            3756..4355
                     /locus_tag="JN701_003677"
     CDS             3756..4355
                     /locus_tag="JN701_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7365980.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4419..>5190
                     /locus_tag="JN701_003678"
     CDS             4419..>5190
                     /locus_tag="JN701_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365981.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKMAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYDIIIPDTRFEG"
BASE COUNT         1213 a         1262 c         1627 g         1088 t
ORIGIN      
        1 tttctcctca ctggataaca tggttgccca gggcaatgtt ctgcctgttc tgtacggtga
       61 aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc acggcagacg aaggggatgg
      121 tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt catgtgaaac cgcctgcggg
      181 cggttttgtc gtttatggag cgtgaggaat gggtaaaggc agcagtaagg ggcatacccc
      241 gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg agtgtgattg atgccatcag
      301 cgaagggccg gttgacggtc cggtggatgg attaaaaagc gtgctgctga acggtacgcc
      361 agtcctggac agcgagggga agacaaactt ttccggtgtt acggtggtgt tccgcgccgg
      421 cgagcaggag cagacaccgc cggaggggtt tgaatcttcc ggctcagaga ctgtgctggg
      481 tacggaagtg aaatacgaca cgccgatcac ccggaccatc acgtcggcaa acattgatcg
      541 tctgcgcctg accttcggtg tgcaggcact ggtggaaaac acctcaaagg gggaccggaa
      601 tccgtcggaa gtccgcctgc tggttcagat acagcgtaac ggtggctggg tgacggaaaa
      661 agacatcacc attaagggca aaaccacttc gcagtatctg gcctcggtgg tggtgggtaa
      721 ccttccgccg cgcccgttta atatccggat gcgcaggatg acaccggaca gcaccacaga
      781 ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa atcatcgatg tgaaacagtg
      841 ctacccgaac acggcgctgg tcggcgtgca ggtggattca gagcagttcg gtaaccagca
      901 ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag gtgccgtcga actataaccc
      961 gcagacgcgg caatacagcg gtatctggga cggaacgctt aagccagcat acagcaacaa
     1021 catggcctgg tgtcggtagg acatgctcac tcatccgcgc tacggcatgg ggaaacgtct
     1081 tggtgcggca gatgtggaca aatgggcgct gtatgtcatc ggccagtact gcgaccagtc
     1141 agtgccggac ggatttggcg gcacggagcc gcgcatcacc tgtaacgctt acctgaccac
     1201 acagcgtaag gcgtgggatg ttctcagtga tttctgctcg gcgatgcgct gtatgccggt
     1261 atggaacggg cagacgctga cgttcgtgca ggaccgaccg tcggataaag tgtggaccta
     1321 taaccgcagt aatgtggtga tgccggatga tggcgcgccg ttccgctaca gcttcagcgc
     1381 cctgaaggac cgccataatg tcgttgaggt gaactggatt gatccggaca acggtcatga
     1441 gacggtgaca gagcttgtgg aggacgcgca ggccattctc cgttacggtc gtaacgtcac
     1501 gaagatggat gcctttggct gtaccagccg gggacaggcg caccgcgccg ggctgtggct
     1561 gattaaaacg gaactgctgg aaacgcagac cgtggacttc agcgtcggcg cagaagggct
     1621 tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat gattatgccg gtatcagcac
     1681 cggtggtcgc gtactggcgg tgaacagcca gacccggacg ctgacgctcg accgtgaaat
     1741 cacgctgccg tcctcctgta ccacgctgat aagcctggtt gacggaaatg gcaatccggt
     1801 cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg aaagtgagcc gggttcctga
     1861 cggcgttgca gaatacagcg tgtgggggct gaagctgccg acgctgcgcc agcgcctgtt
     1921 ccgctgcgtg agtatccgtg agaacgacga cggcacgtat gccatcaccg ccgtgcagca
     1981 tgtgccggaa aaagaggcca tcgtggataa cggggcacac tttgacggtg accggcgcgg
     2041 cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg accgccgaag tcaccgcaga
     2101 cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg aaggtggtga agggtgtgag
     2161 cttcctgctt cgcctgaccg tggcagcgga cgacggcagt gggcggctgg tcagcacagc
     2221 caggacgacg gaaaccacat accgcttcag gcagctggcg ctggggaatt acagtctgac
     2281 agtccgggcg gtaaatgccc gggggcagca gggcgatccg gcgtcggtat cgttccggat
     2341 tgcggcaccg gcagcgcctg tcactattga actgataccg gggtattttc agataacggc
     2401 ggtcccgaaa ctggctgtat acgacccgac ggtgcagttt gagttctggt tttcggaaaa
     2461 gcggattgcg gatatcaggc aggttgaaac cagcgcccgc tatcttggca cggcgctgta
     2521 ctggatagcc gccagtatca atatcaggcc gggccatgat tattattttt acgttcgcag
     2581 tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc ggtcgggcga gcgatgatgc
     2641 ggaaggttat ctgagttttt ataaagggtt gatcaataac acgcatctcg gcaaggagtt
     2701 gtggacgcag attgataacg gtcagcttgc gccggacctg actgaaatca ggacgtccat
     2761 tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa aaactggaaa atcagagtgc
     2821 tgcaatccag cagatacaga aagttcaggt tgatacaaat aataacctga acagcatgtg
     2881 ggcggtgaag ctgcaacaga tgaaggacgg acgcctttat attgcgggta tcggtgccgg
     2941 tattgagaat acgccagcag gaatgcagag tcaggtgctg ctggcggcag acaggattgc
     3001 gatgattaat cctgcgaatg gcaacacaaa gccgatgttt gttggtcagg gcgatcagat
     3061 attcatgaac gacgtgttcc tgaaacgcct gacggctccg accattacca gcggcggtaa
     3121 tcctccggca ttttccctga cacctggcgg acggctgacg gcgaaaaatg ccgatatcag
     3181 cggtaacgtg aacgcgaact ccgggacgct caacaacgtc acgattaata agaactgtcg
     3241 ggttctggga aaattgtccg cgaaccagat tgaaggcgat ctcgttaaaa cagtgggcaa
     3301 acctttccca cgggactccc gggcaccgga gaggtggcca tcagggacca ttaccgtcag
     3361 ggtttatgac gatcagccgt ttgatcggca aattgttatt cccgcggtgg cgtttcgcgg
     3421 tgctaaacat gagcggaaga ataacaatat ttattcgtca tgcagcctga tagtgaagaa
     3481 aaacggtgct gaaatttata accgaacgac cctggataat acgctgatat atacgggtgt
     3541 tattgatatg cctgccggtc acggtcacat gacgctggag ttttctgtat cggcatggct
     3601 ggtaaatggc tggtatccca cagcaagtat cagcgatttg ctggttgttg tgatgaagaa
     3661 agccactgca ggcatcacga ttagctgaat tttataaccc agatacgggc accagaaatg
     3721 gtgccttttt tattgcagaa aagcgagagg taattatgcg taaagtttgt gcagccattt
     3781 tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc atgggcgtct gaacatcagt
     3841 ccacgctgag cgccgggtat cttcagcccc atactgatat gccaggcagc gatgacctga
     3901 agggcattaa cgtgaaatac cgttatgagt ttacggacac gttggggctg gtgacgtcat
     3961 tcagctatgc caacgctgaa gatgagcaaa aaacgcatta cagcgatacc cgctggcatg
     4021 aggattccgt gcgtaaccgc tggttcagcg tgatggcggg gccgtctgtg cgcgtgaatg
     4081 aatggttcag tgcgtatgcg atggcgggcg tggcttacag ccgtgtgtcg accttctccg
     4141 gggattatct ccgcgtaact gacaacaagg ggaaaacgca cgatgtgctg accgggagtg
     4201 atgacaaccg ccacagcaac acgtctctgg cgtggggggc tggcgtgcag tttaacccga
     4261 ccgaatccgt ggccattgac cttgcttatg aaggttccgg cagtggcgac tggcgcactg
     4321 acggtttcat cgtgggtgtc ggttataagt tctgattagc caggtaacac agtgttatga
     4381 cagcccgctg gttcaggcgg gctttttgtg gggtgaatat ggcagtaaag atttcaggtg
     4441 tactgaaaga tggggcgggt aaacctgtcg taaattgtgc gattgaactg cgggccagaa
     4501 gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg cgtgacggac aataacggtg
     4561 cttatgtgat tgaggctgag ccggggtatt acgaggttgc gcttcactgt aacggctggc
     4621 agccaacccg tgtcggggat attgatgtgg caccgactga tgcaccgggg acactgaacg
     4681 cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga ggtgatgaag cgctttgagg
     4741 aaatggtggt gcaggcgcag cagagcgccg gggccgcagc cggaaacgca cagcagacgg
     4801 cgcaggatgt ggcggcagcc gcaggttatg cccgcgcagc agaacaggcc aaaaatgaca
     4861 ttgatgctgc gctgaccggc actctgaaaa tggctaacca tctgtcagaa atcgcagcag
     4921 caggcgaaaa ggcacaacag aagtcccggg ataatctggg gctgaaaagt gcggccacga
     4981 tggaagcaca gagcgacatt tacgaccgga caaaaggccg tctggcgata cccggcgcat
     5041 tcggctttgg gtgtgctttt ctgcctgaag atgttatccg ttttgacact aagagtgatt
     5101 tcctggcctg ggtaaggaat gcgctgccag gtgaatattc cgttgctggc ccctacgaca
     5161 tcatcatacc cgacacacgg tttgaagggg
//
LOCUS       DADLTD010000235         5163 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.239, whole genome shotgun sequence.
ACCESSION   DADLTD010000235 DADLTD010000000
VERSION     DADLTD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.239"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..414)
                     /locus_tag="JN701_003679"
     CDS             complement(<1..414)
                     /locus_tag="JN701_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAY7365982.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMQELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPE"
     gene            complement(405..1463)
                     /locus_tag="JN701_003680"
     CDS             complement(405..1463)
                     /locus_tag="JN701_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY7365983.1"
                     /translation="MADSEFQRLTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATVASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDAISSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            complement(1450..1875)
                     /locus_tag="JN701_003681"
     CDS             complement(1450..1875)
                     /locus_tag="JN701_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7365984.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLRRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(1875..2423)
                     /locus_tag="JN701_003682"
     CDS             complement(1875..2423)
                     /locus_tag="JN701_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HAY7365985.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(2423..3502)
                     /locus_tag="JN701_003683"
     CDS             complement(2423..3502)
                     /locus_tag="JN701_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAY7365986.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALTYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARKRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(3499..4827)
                     /locus_tag="JN701_003684"
     CDS             complement(3499..4827)
                     /locus_tag="JN701_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY7365987.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(4888..>5163)
                     /locus_tag="JN701_003685"
     CDS             complement(4888..>5163)
                     /locus_tag="JN701_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7365988.1"
                     /translation="AQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFM
                     INKASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
BASE COUNT         1081 a         1626 c         1329 g         1127 t
ORIGIN      
        1 ttccggcgca ttcaccgcgt cagtacaggc agatgagcag gtgaacgtgc ttttgtcgta
       61 tcgcgtgatg gtggcgtctg gtctgcccaa ggcagcaagc tgtgcaaggt aaaaatcctc
      121 attgatgccg cccgccaggt taaccttcgc atccagccgt tgctgacgct ggcgaagggt
      181 ctgcgtccct gccggaatac attcatccgg caggccgcac agacgctccc agcggtttat
      241 cagttcggtg gtggtgcgcg gatccagctc ctgcatcagg gcatccgcac gctgatgaac
      301 acgggttaat gacggtgccg caccggcaat cgccggatcg ctggctgacc acgccggacc
      361 gggcggcaac agtgctgaca acagacggat gtaatcatcg tttgtcacgt ccatgaaatc
      421 gcccccagaa ccgccagttc atttttcgca atggtgatat tgtccgccgg tgcaagcaac
      481 tgatggctgt attccccgtt cgcaccggaa atcgcttcac tgatacgtga caccttcagt
      541 tctccctgcg gataaccatc acgcagcagg aacgaacgca actccgcggt gatggcagcc
      601 cgtatttccg gtgtgtccgg cgtcacacgg atatgaaaat ccaccgtatg tgccaccggc
      661 ctgaacacat acaaatcaga gcctgccacc ggggccagtg gcccgatatg ttgtcttgcc
      721 gccgtttccg ttgattcttc cggaatggga ttaatcaggt cactgctggc aatcatcaca
      781 ccgacagtcc ccgttcccat ccagtgtcgg tatgtccatg cgcgggtaat gccaggcact
      841 tctttagccc agacgacata gtccccgtca gccccgccct gcggcgtcca gtaataccgc
      901 tcaatgacgc gggcgcgcca cgtttccagc tcttcagtat caaatccgcc tgtcagggtg
      961 tcagccacac cggaagacgg cagaccattc accggcgtga ccaggattaa tgccgtaccg
     1021 tcgtcagcgt taccgaccgc acctgcactt gagcaggcga tcggcacgcg caggacacca
     1081 ccggagctga ttgcatcgtc agttgtcgtg tactgcacca ggtcatcgcg ctgaataaca
     1141 ctcccggcag tcaccttcag gccatcgctg acaccttccc agcgcatata cccgctggca
     1201 accgtggccc ccttgcgcgg acaccgtttc atcgcagcat gtcgcgccag ccaggactca
     1261 tcgcacaggt caggcagcat attcattgcc agataatcga tgtaaccgta gaccgtatgc
     1321 agcgccgccg catacacctt tgcccgcacg tcttcatcca tgcgccggag cgtgtcgctg
     1381 acgtccagcc tggcgaataa atcgttacgg agcatactga tattttctgc cagcgtcagg
     1441 cgctgaaatt cactgtccgc catgcgttat cgcactccac agatcatcaa aagaaatcat
     1501 tactggtcca tcacgacgcc tgagggtgat actgttaccc agctcattaa tcccggtgcg
     1561 gcggatatcc agatcaatac gggacaccac gccgtcatca gtcatccatt gcaggcattc
     1621 gcggatatat ccccttaccg tctgcaccag ctgattggtc agtttgctgc gctgaagcaa
     1681 ccacagtcgg gagccgtaac ggtcattctg taccgcaggc caggtatccc cccaccatcc
     1741 catcgggacg tcggcattgt catcaggctc cgcccgccgc caggtgaaca gggaaatcac
     1801 cacggcgcgg gtcagcggat ccagcggtgc gctggcgcag gtgcgtttac cgttcaccgt
     1861 cagccacagt tccatcatgc ctccatcgct ttatcaggtt tgtcggtgtt actgccctga
     1921 ccgttctctc tgtgacgatg cccgttatag gcaagccgca tcgctgacat ggtggtgccg
     1981 ccggagtcgc acaggtcttt cacctgtccg gtcacttcca gatccatttc aaaacgtgct
     2041 tcaggtgcat tgcgaaacgt gatcgttttc cctgcaccgt ccaccacgat cccctcccgg
     2101 gtcagcgtca cagactgccc ctgatcgtca tagacagcca cctcacccgt ttgcagccct
     2161 ttcaggcggt agcgccggtc cgacaccgta acaaccaccg catgagaacg gtcgccatcc
     2221 ggaaacaaca ccaccgcttc cgcaccgctg tttgcccttg cggtaaaacc gtagggttca
     2281 agatgttcaa ccccggcttt gggttcaccg gcaatcaggg acacatccac ggtctgacat
     2341 ttcgtggcgg cactgatgct tttcaccact gcccgcccaa tcaggccgag aagttgtcgc
     2401 tgcatggctt caatcgtcct catcagaacg ggtcctcctg tactctggct tttttctttt
     2461 tccgcgcgcc gggggcttcg ggttcaggca gataagcgtc aggcgggccg acacggattt
     2521 ccgtcagggt gccgttctgg tcctgagtaa acgtgacttc cgagacaagc agttcggtat
     2581 tgtcgaaacc acagaccgga tcgaagacaa tcacccgctg gttgggctgc cacagcgtac
     2641 cgttaccctg tcgccagccc tgcaccacat aggtggtttc atccgtccgc gccgcccgtt
     2701 ttcgggcttc aaagtcagca cgcgcaatac agcctgcccc cgtggcctgc cctgtctgcc
     2761 tgatatacat cggacggtaa cgggcaataa atgcgtcctc tgtgcgggcc cgcagcgcgg
     2821 tggtggtggc ctcaccgaaa tcatcgtcgt ttccggcacg ctgccccgcc acctggtaaa
     2881 cagaaaaccg ctcccggata ctcttctccg tatcacagga aaggatgttt tccccaagta
     2941 ccagcgcggt atgtgcccgc gttgagccaa taccgccaat caccagcctg ccgtgcgggt
     3001 cgtcgtaagt cagcgcctgc tgctgaccga gtattttgtt gattacctca atcaccgttt
     3061 caccgtgatc aggctgaaca tcaggaataa cacccgacgg cgcaccgctg ttcaccacct
     3121 caatgccgaa aggcgcagca agcgcctgcg caatctgcac cagcgagcgt ccgttaaact
     3181 gtgtcggttc ggctgcacag tcaatcaggt cagccgtcag actacgtccg gcaataccgg
     3241 tgctgaccga acgggcatcg taacgaacgg gagtcgcctc cacccagccg gtgatcacca
     3301 gctcatcacc aatcagcact tccacttttg aaccgttttt aatgcgcggc tgaagcgtgg
     3361 tgataccctc atctcccggc cattggcgag tgatctccac actgaaatcc cgcgccagcc
     3421 gttcaatacc ggcaccgatg cgcaccgatg tccagccatt ccactcccgg ccatttaccc
     3481 gtagcgtgac attgtcgttc attgcactgg caccttcaga gggatcaccg gcacaaagcc
     3541 gggatgcgta atggcattac gccgaataat gtccgcgtca cgcgccgcgt tatcaaacca
     3601 ggtcgccgcc agcaccagcg cgggtaaaac ctcatccggc gtgcgctgaa tgatccgtgc
     3661 agactgttca aggcgcgtgt tgatatccgc attcagatct gctttcaccc ggcgcagcgc
     3721 cagaaacagc gcatcactgg ttgtacggga caactcctta tcaattgccg tattcagtgt
     3781 gtcgcgaatg tcggtcagtt cttcccacgt tggcaggtca accgtgtttt tcaccgccgg
     3841 tgcattgttc agtgccggat gcgtgacaga aggccagccg gtgctctgcg cgggtgttgt
     3901 tgactgccct actgtggcat tctgcatcac cgcggaagtt gtgggcgcag gcaatcgtgt
     3961 gacggcatac gccgcttcgc tgattgcggt cgtacgaagg gtgctggcaa ccacgttacg
     4021 ctgctgcgtc gccgtggcgg tagttttact gtccgttttc cagacgccgc gcggttgcag
     4081 atcgctgccg aggctgacac cggaaagcgt tttgatcatg gtgaccaggt cgctggcgtt
     4141 accataaagg cgtttcccgg tacgccacat tttctgcacc tgctcaacga aatttttgcc
     4201 tgacgatggt ggcggcagaa gtaccgagat atccccctgc aacagcctgg cggcatccga
     4261 tacggcagaa tccaccactt tcatcgcatc agaaacatac ccaagcattg tgctggcatt
     4321 accgacgacg tcgttctgca caaaatctgc cacgccatcg atactgaaac cactgaagct
     4381 gtcactgatg cagtcatcca gtgcagaaca ggatgacatc agcgtctgcg ccgtcgccgc
     4441 acctgaagtg gggtaagaga gttctcccgc ttcgacaaac ttcaggtcaa agcggacaat
     4501 acgcccttca ctcttcgatg tgctgacccg aacttccccg tcaacacaga ctttcagctc
     4561 accgtaagtc ggatggacaa gcgtgccggg accgggttta ttcagcgcgt caatcaggcg
     4621 atcgcgctgg tcaaagcagt catctcccac cacataagct gtgatggacg ggcggaaagt
     4681 gattttcccc aggtcttcgg tatagggttt gtcgcggttc gggtattcgt gcgtttccac
     4741 acgacgaccg gttcccgcac tttcttcttc aaccttaaac ggcacaccgc gaaatgacgc
     4801 gtcctgaagt ctgtctttcc acgtcatata aactccgtac ataaaaaatc ccaccggagt
     4861 gggactcatt aacagattaa tttttcatta cctgccaaag cgcgtatagc caacatcatg
     4921 gctgacatca aaaccgctgg atcgcgtttc cataacccgc atacccggag gcgaattcac
     4981 aaaagagacc ttgatctcac catcaacttt tggcgcagaa gctttgttaa tcatgaaggg
     5041 attcgggcct gtggcaccgg aggcgttgtt tgactgagcc ggatctaccg cctgataagg
     5101 tgtgtatccc cgtgccggta ttcccgtccc ataagcatca taagcacccg cgccccactg
     5161 cgc
//
LOCUS       DADLTD010000236         5087 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.240, whole genome shotgun sequence.
ACCESSION   DADLTD010000236 DADLTD010000000
VERSION     DADLTD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.240"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..1203)
                     /gene="ascF"
                     /locus_tag="JN701_003686"
                     /pseudo
     CDS             complement(1..1203)
                     /gene="ascF"
                     /locus_tag="JN701_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
     gene            1463..2473
                     /locus_tag="JN701_003687"
     CDS             1463..2473
                     /locus_tag="JN701_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7365989.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            2622..3149
                     /gene="hydN"
                     /locus_tag="JN701_003688"
     CDS             2622..3149
                     /gene="hydN"
                     /locus_tag="JN701_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY7365990.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            3302..>5087
                     /gene="hypF"
                     /locus_tag="JN701_003689"
     CDS             3302..>5087
                     /gene="hypF"
                     /locus_tag="JN701_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7365991.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCG"
BASE COUNT         1171 a         1302 c         1454 g         1160 t
ORIGIN      
        1 cgcgccgcaa ataaaaccgc tgataagact ggcgataagc ggacgtttca ggcggatcgc
       61 cacgccgtat aacgccggtt cggaaatccc cgccataatg gctgatgccg ccgcagccag
      121 cgccgtctgg cgcagttccg ggtttttcgt tttccacgcc accgccagtg atgaaccgcc
      181 cagcgacagg ttagcgccga tctctgacgg catgaccatc ccttctttgc cggtttcggc
      241 aatggtctga atgatggttg gcgtaaagac gcggtgcatc ccggtcatta ccagcagagg
      301 ccacagcgcg cccataatgg cgactgaaag ccagcccaga taaccatgaa tgctgtaaac
      361 cagcgccgaa atggcgctac cgatccagat accaatcggg ccaatcagca ggattgccag
      421 cggtgcggca atcaacacaa tcagcattgg cttgaggaag tttttggtca ccgccggtgt
      481 aatgcgatcc acccagcgtt cgatatatga caggcaccag gtcatcacca gcgccgggat
      541 cactgtgtag gtgtatttca ccgcggtgcc cggaatcagg gcaaattcga catgttcacc
      601 ctaggccgct ttcgccatca gttcaataaa actcggatgt accagcacac ccgcgatggc
      661 aatcgccagc gacatattgg ttttaaattt gatggcggca gatgccgcga ccatcagcgg
      721 caggaagaag aaagcaccgt caccaatcac attcagaatg gttaaggtcg gcgatccttt
      781 tgtcagcacg ccgctcatct cgaggatcat cgccagcagt ttgaccatcg atccgccgat
      841 aatcgccggg atcagcggtg acatggtgcc gatcagcgca tcgaggatcc ccgcaccaat
      901 gcgacgcggc gtgagtttgg gtttacccac gggctgtgcg ggctgcatat ctcccggcag
      961 caggctgacg acttcctgaa aggcttgtga aacggtattg ccgataatca cctgacactg
     1021 gttgtcacta cgtaccacgc cgagcacgcc ggggatggtt tttaacgtcg ggctgtcgat
     1081 aagctggtca tctttgataa caaagcgcaa acgcgtcata cagtgcgtga ccgccgagat
     1141 gttatcaacg ccgcccagtg ccgctatctc cgagcctgcc agagccgcat aatttttggc
     1201 catcggattt tatcctgtta tcagtagggt agatgtagtt gttatcagtc gacgtaacgg
     1261 aaaaatgccg atgctgaaac ggaattagga aaccggtttc acaaaatcat gaatagttgt
     1321 cactaaataa gcaagagagt taattattca ttttttatat ttgtgaaacc ggtcacctga
     1381 atatttttcc cgccgattgc gcaaggacaa tcggcaaacg gtgcagtaca ctgcgttgat
     1441 tgcgtgtaaa gggatgaaaa tgatgacgac gatgctggaa gtggcgaagc gcgccggggt
     1501 ttcaaaagcg accgtttccc gcgtgctttc aggtaatggc tacgtcagcc aggagactaa
     1561 agatcgcgtg tttcaggcgg tagaagagag cggttaccgc ccaaacttgc tggcgcgcaa
     1621 tctgtcggcg aagagtactc agacgctggg gctggtagtg accaacacgc tttaccacgg
     1681 catttatttt agtgaattac tctttcatgc cgcgcgaatg gcggaagaga aagggcggca
     1741 gttgctattg gcagatggta aacacagcgc cgaagaagag cgccaggcga ttcagtatct
     1801 gctggatttg cgctgcgacg ccattatgat ttacccgcgt tttttaagcg tggatgagat
     1861 cgatgacatc attgacgcgc acagtcagcc gataatggtg cttaatcgcc gcctgcgcaa
     1921 aaacagcagc catagcgtct ggtgcgatca taaacagacc agctttaatg ccgtggcaga
     1981 gttgataaac gccggacatc aggagattgc tttccttacc ggctcgatgg attcccccac
     2041 cagcattgaa cgtcttgctg ggtataaaga cgcgctggcg cagcatggta ttgcgctcaa
     2101 tgaaaaactt atcgctaacg gtaaatggac gcccgccagc ggggccgaag gggtagaaac
     2161 gttgctcgaa cgtggggcta aatttagcgc gttagttgcc agtaacgacg atatggcgat
     2221 aggtgcgatg aaagcgttgc acgagcgcgg cgtagcggtg ccagagcagg tgtcagttat
     2281 cggattcgat gatatcgcaa ttgcccccta tatcgttccg gcgctctcca gcgtaaaaat
     2341 tccggtgact gagatgattc aggaaattat tggacggctg atttttatgc tcgatggcgg
     2401 ggatttctca ccgccgaaaa ccttcagcgg aaaactgatc caccgcgact cactcattgc
     2461 tctttcgcga taaacattgt catcacatgt catcgtcact agtgtcgatg acatcttttt
     2521 tcctgcatta cagaaattaa tttccttaaa taacagtgaa ttaaaaactg gcatgatttg
     2581 tgaatgtatc ggcgcattaa ctgtcattgc tggagaattt gatgaaccgt ttcatcattg
     2641 ctgacgcgag taaatgtatt ggttgccgta cctgtgaagt ggcgtgcgtg gtttctcatc
     2701 aggaaaatca ggactgtgca tcgctgaccc cggaaacatt tttaccgcgt atccatgtca
     2761 ttaaaggtgt gaacatttcc acggcgacag tttgccgtca gtgtgaagat gcaccgtgcg
     2821 ctaacgtctg cccgaatggt gctatcagcc gtgataaagg gtttgttcat gtgatgcagg
     2881 aacgttgcat tggttgcaaa acctgcgttg tggcttgccc gtatggcgcg atggaagtgg
     2941 tggtacgtcc ggtgattcgc aacagcggcg cggggctgaa tgtacgggct gacaaagccg
     3001 aagccaataa atgtgacctg tgcaaccatc gtgaagacgg cccggcgtgt atggcggctt
     3061 gcccgactca tgcgctgatt tgtgtcgatc gtaataaact tgaacaactg agtgcagaaa
     3121 aacgccgccg tacggcgctg atgttctaaa aatcttacag aactgttctg gcatcaaagg
     3181 aattcccgga aatattcgta gaatacggag caggcggtat cgccgacctg tgactgccgg
     3241 atgcgacgct gtcgtgtccg gctgggcggg ttacgcaatt catgatggcg ggaataactc
     3301 aatggcaaaa aacacatctt gcggtgtcca actgcgtatt cgtggcaaag tgcagggcgt
     3361 cggttttcgt ccgtttgtct ggcagctggc acagcaatta aatcttcacg gcgatgtctg
     3421 taatgacggc gatggcgtag aagtccggct gcgggaagac ccggaaacgt ttcttgttca
     3481 attgcatcag cactgcccgc cgctggcgcg tattgatagc gtcgagcgtg agccgtacat
     3541 ctggtcacaa ctgcccactg agttcaccat ccgccagagc gcgggaggtg ccatgaatac
     3601 gcaaattgtc ccggatgccg ctacttgccc tgcttgcctt gccgaaatga ataccccagg
     3661 cgaacggcgt tatcgttatc cgtttatcaa ctgtacccac tgcggcccgc gtttcaccat
     3721 tattcgcgcc atgccttacg accgcccgtt taccgtgatg gcggcgtttc cgttgtgtcc
     3781 ggcttgtgac aaagagtacc gcgacccgct cgatcgtcgc ttccacgccc agccggtggc
     3841 ctgcccggag tgtggcccgc atcttgaatg ggtaagtcat ggtgaacatg cagaacaaga
     3901 ggcggcatta caggcggcta tcgcacagtt aaaaatgggc aacattgtcg ccatcaaagg
     3961 gattggcgga tttcatcttg cctgcgatgc acgtaacagt aacgcggtgg cgacacttcg
     4021 ggcgcgcaaa catcgcccgg cgaaaccgct ggcggtcatg ttgccagtgg ctgacggttt
     4081 accagacgct gcgcgccagt tgcttaccac gcccgccgcg ccgattgtgc tggtggataa
     4141 aaaatacgtt cctgagcttt gtgatgatat cgcccctgac cttaacgaag tcggggtaat
     4201 gttgcctgcg aacccgctcc agcatttgct gttacaggaa ctgcaatgcc cgctggtgat
     4261 gacctccggc aacctgagcg gtaaaccacc agctatcagc aacgaacagg cgctggcgga
     4321 tttgcagggc attgccgacg gattcttgat acataaccgc gacatcgtgc agcggatgga
     4381 tgattcggtg gtgcgcgaaa gcggcgaaat gctgcgccgt tcgcgggggt atgtgccgga
     4441 tgcgctggct ttgcctccgg gctttaaaaa tgttccgcct gtgctgtgtc tcggagcgga
     4501 tctgaaaaat accttctgcc tggtgcgcgg tgaacaagcg gtgttgagtc agcatctggg
     4561 cgatttaagt gacgatggca tccagatgca gtggcgcgaa gcgttacgcc tgatgcaaaa
     4621 catctacgat tttaccccga aatacgttgt gcatgacgcg catccgggct atgtctccag
     4681 ccagtgggcg cgtgaaatga atctgccgac gcaaacggta ctgcatcatc atgcccatgc
     4741 agcggcgtgt ctggcagagc atcagtggcc gctggatggc ggtgatgtca ttgctttgac
     4801 gctcgacggt atcggtatgg gggagaacgg cgctttgtgg ggcggcgagt gcctgcgggt
     4861 gaactatcgc gaatgtgagc acctgggcgg cttgcctgca gtggcgcttc cgggtggcga
     4921 tttggcagcg aagcagccgt ggcgaaacct gctggcgcag tgcctgcgct ttgtgccgga
     4981 gtggcagaat taccctgaaa cagcaagtgt gcaacagcaa aactggagcg tgctggcgcg
     5041 ggccattgag cgtggaatta acgcgccgct ggcgtcatcg tgtgggc
//
LOCUS       DADLTD010000237         4960 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLTD010000237 DADLTD010000000
VERSION     DADLTD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.241"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..247)
                     /locus_tag="JN701_003690"
     CDS             complement(<1..247)
                     /locus_tag="JN701_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HAY7365992.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAN"
     gene            505..1284
                     /gene="deoC"
                     /locus_tag="JN701_003691"
     CDS             505..1284
                     /gene="deoC"
                     /locus_tag="JN701_003691"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY7365993.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1411..2733
                     /gene="deoA"
                     /locus_tag="JN701_003692"
     CDS             1411..2733
                     /gene="deoA"
                     /locus_tag="JN701_003692"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY7365994.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAELQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2785..4008
                     /gene="deoB"
                     /locus_tag="JN701_003693"
     CDS             2785..4008
                     /gene="deoB"
                     /locus_tag="JN701_003693"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7365995.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4088..4807
                     /gene="deoD"
                     /locus_tag="JN701_003694"
     CDS             4088..4807
                     /gene="deoD"
                     /locus_tag="JN701_003694"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY7365996.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1193 a         1263 c         1365 g         1139 t
ORIGIN      
        1 cgttcgccag aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac
       61 cttcaaacat atcgcagatt acaccctcat ccagcgcgcg gcgggcttcg gcaggaagct
      121 gcggataagg cagattgttt tctgcttcca gagccagaaa atggcgcttc tgctccgggc
      181 taagcactgg gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag
      241 tgggcatctt cttttccttt tatgccgaag gtgatgcgcc attgtaagaa gtttcgtgat
      301 gttcactttg atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt
      361 tgaaccagat cgcattacag tgatgcaaac ttgtaagtag attaccttaa ttgtgatgta
      421 tatcgaagtg tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta
      481 ttggcgacaa gccaggagaa tgaaatgact gatctgaaag caagcagcct gcgtgcactg
      541 aaattgatgg acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg
      601 tgtcatcaga ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt
      661 atcccgattg ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg
      721 gtaaccaact tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg
      781 gcaatcgcct acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg
      841 ggtaacgagc aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg
      901 aatgtactgc tgaaagtgat catcgaaacc ggcgaactga aagacgaagc gctgatccgt
      961 aaagcgtctg aaatctccat caaagcgggt gctgacttca tcaaaacctc taccggtaaa
     1021 gtggcagtga acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg
     1081 ggcgtagaaa aaaccgttgg tttcaaaccg gcgggcggcg tgcgtactgc ggaagatgcg
     1141 cagaaatatc tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac
     1201 taccgctttg gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac
     1261 ggtaagagcg ccagcagcta ctaagcaaga tgctttacgc ctgatgcgct gcgcttatca
     1321 ggcctacgag acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc
     1381 gcctcatact cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa
     1441 aaacgtgatg gtcatgcgct gagcgatgaa gaaattcgtt tctttatcaa cggcattcgc
     1501 gacaacacta tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat
     1561 atgacaatgc ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc
     1621 gactggaaaa gcctgcatct gaatggcccg attgttgata aacattccac cggcggcgtc
     1681 ggcgatgtca cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg
     1741 atgatctccg gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc
     1801 cctggcttcg acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc
     1861 gtggcgatta tcggtcagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc
     1921 cgtgatatta ccgcaaccgt ggactccatc ccgctgatca ctgcctcgat cctggcgaag
     1981 aaactggcgg aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt
     2041 atgccgacct acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc
     2101 gctggcgtgc gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca
     2161 ggtaacgcgg ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg
     2221 cgtctgtttg atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg
     2281 aaagatgacg ccgaagcgcg cgcggaattg caggcggtgc tggacaacgg taaagcggca
     2341 gaagtctttg gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac
     2401 gcgaagtatc tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt
     2461 gtcagtgaaa tggatgcccg cgcgctgggg atggcagtgg ttgcaatggg cggcggtcgt
     2521 cgtcaggcat ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc
     2581 gaccaagtag acggtcagcg tccgctggcg gttatccacg cgaaagacga aaacagctgg
     2641 caggaagcgg cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc
     2701 acaccaactg tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt
     2761 tgaagcactg agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc
     2821 ggcatcggcg ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt
     2881 catatcgcag aagcttgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat
     2941 ctgccaaatc tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt
     3001 ccggcgggaa tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg
     3061 tcatccggta aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt
     3121 gagtggggat acttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg
     3181 gtcgaacgcg ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt
     3241 ctggatcagc tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct
     3301 gactccgtgt tccagattgc ctgccacgaa gaaacctttg gcctggataa actctacgaa
     3361 ctgtgcgaaa tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc
     3421 gctcgtccgt ttatcggtga caaagccggt aacttccagc gtaccggtaa ccgtcacgac
     3481 ctggctgttg agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt
     3541 caggtggttt ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa
     3601 gtgaaagcaa ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg
     3661 ggtgataaca ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt
     3721 cgcgacgtcg ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg
     3781 atgtctttgc tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg
     3841 acctggaccg gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa
     3901 gtaaaaccgg gttcactggg tcaccgtgaa accttcgcgg atatcggcca gactctggca
     3961 aaatattttg gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg
     4021 cttgatgccc tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga
     4081 taaaacaatg gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt
     4141 gatgccaggc gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg
     4201 tgaagtgaac aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat
     4261 ttccgtaatg ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat
     4321 caccgatttc ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca
     4381 cgtaaaactg cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg
     4441 catccgtttt aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc
     4501 ggtagacgcg gctaaagcac tgggcattga tgctcgcgtt ggtaacctgt tctccgctga
     4561 cctgttctac tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcatcctcgg
     4621 cgtggaaatg gaagcggctg gtatctacgg cgtcgctgca gaatttggcg cgaaagccct
     4681 gaccatctgt accgtgtctg accacatccg cactcacgag cagaccactg ccgctgagcg
     4741 tcagaccacc ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc tgggcgataa
     4801 agagtaattg tgtttcgctg aaaggcgatt gtcgtgtgaa gccggagtgg gagactgctc
     4861 cggcttttta gtatctattc atcaatagca actgatttat agatttacta ttatttttca
     4921 tatggataga aaataaaatc tattcaagta tctattcacg
//
LOCUS       DADLTD010000238         4960 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLTD010000238 DADLTD010000000
VERSION     DADLTD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.242"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            388..1362
                     /gene="hemB"
                     /locus_tag="JN701_003695"
     CDS             388..1362
                     /gene="hemB"
                     /locus_tag="JN701_003695"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY7365997.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1468..2319)
                     /gene="tauD"
                     /locus_tag="JN701_003696"
     CDS             complement(1468..2319)
                     /gene="tauD"
                     /locus_tag="JN701_003696"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY7365998.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(2334..3143)
                     /gene="tauC"
                     /locus_tag="JN701_003697"
     CDS             complement(2334..3143)
                     /gene="tauC"
                     /locus_tag="JN701_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY7365999.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAA
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            complement(3140..3907)
                     /gene="tauB"
                     /locus_tag="JN701_003698"
     CDS             complement(3140..3907)
                     /gene="tauB"
                     /locus_tag="JN701_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY7366000.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3920..4882)
                     /gene="tauA"
                     /locus_tag="JN701_003699"
     CDS             complement(3920..4882)
                     /gene="tauA"
                     /locus_tag="JN701_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7366001.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKLGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1180 a         1415 c         1247 g         1118 t
ORIGIN      
        1 tggacatata taatttatgg agaaaaacac gcatcaaatt gataaaagga cgatatttca
       61 acatgttgca gataatgtta tttttgatac atacgcgtta taaatatcaa aaacacaaac
      121 cagataaata tcttatcaat atacaacaaa gtttgaatta ttcaatttat agcagcacaa
      181 ccaaacgcaa aaaaataata accaagagct ataaataaag attaaaaata ttttcagtga
      241 atatgaactt attgctattt ctattacaaa gatgtcctgg tctctctttc attctcttct
      301 tgtcaacatc acgacaactt tcgtaaaaca ctcctgcccc gcttcaggta tactgtgcac
      361 cttaattcca caaacatcag gcagaccatg acagacttaa tccaacgccc tcgtcgcctg
      421 cgcaaatctc ctgcgctgcg cgctatgttt gaagagacaa cacttagcct taacgacctg
      481 gtgttgccga tctttgttga agaagaaatt gacgactaca aagccgttga agccatgcca
      541 ggcgtgatgc gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat cgccaacgcc
      601 ggtattcgtt ccgtgatgac tttcggcatc tctcaccata ccgatgaaac cggcagcgac
      661 gcctggcggg aagatggact ggtggcgcgt atgtcgcgca tctgcaagca gactgtgcca
      721 gaaatgatcg tcatgtcaga cacttgtttc tgtgaataca cttctcacgg tcactgcggt
      781 gtgctgtgcg agcatggcgt cgacaacgac gcgactctgg aaaatttagg caagcaagcc
      841 gtggttgcag ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat ggacggccag
      901 gtacaggcga ttcgtcaggc gctggacgct gcaggcttta aagatacggc gattatgtcg
      961 tattcgacca agttcgcctc ttccttttat ggcccgttcc gtgaagctgc cggaagcgca
     1021 ttaaaaggcg accgcaaaag ctatcagatg aaccctatga accgtcgcga ggcgattcgt
     1081 gagtcactgc tggatgaagc ccagggcgca gactgcctga tggttaaacc tgctggagcg
     1141 tacctcgaca tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg cgcgtatcag
     1201 gtgagcggtg agtacgcgat gattaagttc gccgcgctgg cgggtgctat agatgaagag
     1261 aaagtcgtgc tcgaaagctt aggttcaatt aagcgtgcgg gtgcggatct gattttcagc
     1321 tactttgcgc tggatttggc tgagaagaag attctgcgtt aatttttttc ctctcagaaa
     1381 gggggagtta acctgttgaa agtacggtga acgccccctc accctaaccc tctccccaag
     1441 ggggcgaggg aaccgtccgc actcgtatca ccccgcccga taaaacggtt tatccccaag
     1501 aatcgtcacc cgatgcataa tccgccgctg tggcaggtaa tcggcattgg catagtgctg
     1561 agtcacgcgg ttatcccaaa tcgcaatatc attcggttgc cagcgccaac gcacctgaaa
     1621 ctccggttta gtgatatggg cgaataaaaa gcctaacaag gcttcgctct ctttctcgct
     1681 gacatcgaca attcgcgtag taaagccttc attcacaaac agcgcctgtt taccgctcac
     1741 cggatgcgtt cgcaccaccg gatgtagcaa cggaggattt ttcgcgaccg cctcgcgcca
     1801 gcgttgatgt tcctcctcgg ttttgcggta tttgtattcc gggaacgatt tacggaaatc
     1861 atgctccgca cgcagcccgc tcagcagctg gcggaaggga acagagagcg cctcataggc
     1921 cgtaataccg ctggtccaga gcgtatcacc gccggtcgaa ggtaattctt tagctgccag
     1981 aatcgcccct gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt tgtcgttatc
     2041 tggcggatta tcgttatggg tatccagcac gatgatctcg tcaacccctt cggcatgagg
     2101 gtaaacaggg tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt gctgctgcgg
     2161 cgtaatggct tgatcgcgca gaaacaccac ctgatggcgc agcaccgcat ggtaaagctg
     2221 ttcaaactga ttatcgctta acgggcgcgt caggtcggca cccgaaattt gtgcgccgat
     2281 atacggcccc agcggggtaa tgctcagacg ttcactcatt gtacttctcc atgctagggc
     2341 gtcagacggc gctgtaacgc gcgcaaaccc agttctaaaa gaaaggcgat aatcgcaatc
     2401 accgcgattc ccgccagcac cacgtcagtc gcgagaaatt cacccgctga ctgaaccata
     2461 aatcctaaac cgcgcgtcgc ggcaatcagc gccgccgcta ccagcgtaga ccagcccaca
     2521 cccagaccaa tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa aatgacaaac
     2581 cacagcacct gcgcacggct ggcacccagc gactgggcgg cacgaatgcg aacctgctgc
     2641 gcgcttttca cccccgccag cgccgacatc gccaccggtg caaaaatcgc taaatagatc
     2701 agtaagatct tcgaggtttc accaatacca aaccagatca ccatcagcgg caaataagcc
     2761 agcggcgaca ccggacgata aagttcgatt accggatcca gaatgccgcg caccgtcggg
     2821 cttagcccca tggcgatccc gaccggaata ccgatcacca ccgccgcgag tagcgccagc
     2881 acaatgcgcg tcaggctggc tgccagatgc tgccacagcg tggcgtccat aaatccctgc
     2941 ggtccggcaa tggtgagcag ttttgccagt acttgttgcg gcggcggcaa aaacagcggg
     3001 ctaatcagtt gaagcgccgc caccgtccac catacagtga gtaaaaccgc taacgtgcca
     3061 atgcttaagg tcacctgacg cgagagcggc cagcgccatt tcagccgccg cgaatgcagt
     3121 ttttcattaa tgagcacact catgagaacg cctcccgttg ctcaaatacg cggcttaaaa
     3181 catattcgcg catggcgatg aattgtggat cggacttgat gctgcggctc gactctcccg
     3241 caacaaagcg gcgagcaaag ttgagcggca gccgctccag cacacggcca gggccggatg
     3301 aaagcagaac cagttcagtc gccataaaca ccgcttcttc tatatcgtgg gtaatcaaca
     3361 gcacctgctt gcccgtctcc tgccagagtt tcagcagcag agtttgcatc tggtcgcggg
     3421 tgaaggcgtc cagcgcacca aacggttcgt cgagtaataa cagctgggga ttcgccgcca
     3481 gcgcgcgggc aatccccacc cgctgacgtt gaccaccgga aagctgccag atatagcgtt
     3541 tttctgcgcc ttccagcccc acttttttca gcacctggtg cgcgatttcc agtcgctgca
     3601 ttttctctat acctgccaat tgcaggccga acgccacgtt gtcctgtaca ttgcgccacg
     3661 gtagcagccc ttcattctga aaaaccaccc cgcgctctgc tcccggtccc tcaatacgct
     3721 tacccgccag ttgaatgctg ccatgctgat aaggcacaaa accggcaatc agattcagca
     3781 acgtggtttt accgcagccg gacggcccca gcaccaccag tagctcgccg ctttccagcg
     3841 tcaggttgat atcttccagt gccggtttgc cgccataatc ggcatagaga tgagagattt
     3901 gcagcatctg cgcctccttt tattgcacga agcgcgaggt aacgtactgg ctgtaatcat
     3961 tcgctacagc cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg atgattactt
     4021 tgttcaccgg tccggtcagt tctgccgttt gttgctgcgg cgtcagatag gtattcccct
     4081 taaccagccc cggaacgtca ccttccggca cgccgcttaa acgcgccagt ttgctgatgt
     4141 tttccggctg tttcagccac gcgtctgggt tagcaatgta cggttgctga gcatcgatgg
     4201 cgcttttagc gaacgctttc acgacctcag gatgtttctc ggcaaaatct ttgcgcacca
     4261 cccagacgtc cagcgttggc gcaccccact gcccgacctg ttcagaatcg gtcaacacct
     4321 tgccgtcttt ttccagggcg ttaaccgccg gtgcccagac ataagcacca tcaatatcac
     4381 cccgctgcca tgcggcgata atcgcgggcg gctgcaggtt cacaatctcc acttgcccta
     4441 gtttaatccc ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg gagataaacg
     4501 gtacggcaat gcgtttgcca atcaaatctt cgggtttgct gatagttttc tttaccacca
     4561 gcgcttcgga gttacccagt tttgacgcca gcaagaagac ttcaatcggc acctgttggc
     4621 tggctgcaac cgctaatggg ctggaaccga ggttgccgat ttgcacgtcg ccagaagcca
     4681 gcgcccgcac gatgctggct ccgctgtcaa acttacgcca gtccacgctc gccccgctct
     4741 ctttagcaaa ggtgttgccg gcctgagcca ctttcgccgg ttcggctgag gtttgatacg
     4801 ccacggtgac gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg gcaagaagtg
     4861 tgttacgcga tgaaattgcc atgattgtct gctccccagt cttgttatgg gagcagtatt
     4921 caggaataaa aacattcatt aaaagaatta gtcgttatcg
//
LOCUS       DADLTD010000239         4880 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLTD010000239 DADLTD010000000
VERSION     DADLTD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.243"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            239..805
                     /locus_tag="JN701_003700"
     CDS             239..805
                     /locus_tag="JN701_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7366002.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            856..3315
                     /locus_tag="JN701_003701"
                     /pseudo
     CDS             856..3315
                     /locus_tag="JN701_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3330..4061
                     /locus_tag="JN701_003702"
     CDS             3330..4061
                     /locus_tag="JN701_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7366003.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGILPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            4058..>4880
                     /locus_tag="JN701_003703"
     CDS             4058..>4880
                     /locus_tag="JN701_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7366004.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVPPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYT"
BASE COUNT         1357 a         1200 c         1101 g         1222 t
ORIGIN      
        1 atcgatgatt tttaatcatc tatcttcctc tcagttacat gaataaacca ccaaatatag
       61 cgcaccccta taatttccaa tactttattc atcaaacatt taattcatac catcaattaa
      121 ttacttttac atttaataaa attaataata tttttaagaa aaactaaaaa cattcgacaa
      181 tcaaataaga tattcttgca ccgatatact tttttctttt ctgttaaagg actcagttat
      241 gtttaaagga caaaaaacat tggccgcact ggccgtatct ctgctgttca cagcacctgt
      301 ttatgcggct gatgaaggat ccggtgaaat tcactttaaa ggtgaagtta ttgaagcacc
      361 gtgtgaaata catcaggatg atattgataa agaggttgaa ctcggtcagg tgaccaccag
      421 ccacattaat cagtcacatc acagcgatgc cgttgctgtc gacctgcgct tagtcaactg
      481 tgatctggaa aactccagca acggttccgg tggcaagatt tctaaagttg cagtcacctt
      541 tgatagctca gcgaaaacca ccggcgcaga tccgattctc aacaacacca gtacgggtga
      601 agccaccggc gtcggcgtac gtttaatgaa taaagatcaa agcaatatcg ttttaggcac
      661 cgctactcca gatatcgacc tggctccgac ctccagcgaa caaacgctga atttctttgc
      721 ctggatggaa caaattgatc aggctacacc tgtaacgcca ggcgcagtta cggcgaatgc
      781 aacctacgtg ctggattata aataagtatt actttggcgg cgctatcgcc gcctttcata
      841 aaatatttat caggtatgga caccgtgaat atttatcgac tctcttttgt atcctgcctg
      901 gtcgtggcga tgccttgcgc attggcggtc gaattcaacc ttaatgttct cgataaatcg
      961 atgcgcgacc gcattgatat ttcattatta aaggaaaaag gagtcattgc tcccggtgag
     1021 tattttgtta gcgttgcggt aaataataac caaatcagta acgggcaaaa gattaactgg
     1081 cacaaaaatg acgataaaac cattccgtgc atcaatgatt tactggtcga taaatttggc
     1141 ttaaaacctg aagtccgtca gtcgttacca ttgataaatc agtgcgtcga ttttagctcc
     1201 cgacctgaaa tgctcttcaa tttcgatcaa gccaatcagc aactgaatat tagtattccg
     1261 caagcctggc tggcgtggca ctcagaaaac tggaccccac cctccacatg gaaagaaggt
     1321 gtcgccggtg tcctgatgga ttacaacttg tttgccagca gctaccgccc acaggatggc
     1381 agcagcagca ctaatcttaa cgcctatgga acagccggaa tcaacacggg ggcatggcgc
     1441 ttacgcagtg attatcagtt gaatcagact gatagcgatg ataaccatga acagtcaggc
     1501 gaaatatcac gtacctatct ttttcgcccg ttaccgcaat taggttcaaa attaaccctc
     1561 ggcgaaacgg attttagttc caatattttc gacggttttt cttataccgg tgcggcactg
     1621 gcaagtgacg atcgaatgtt gccatgggaa ctacgcggct acgccccaca aattagcggt
     1681 attgcacaga ccaatgccac ggtgacgatc agtcaatcag gccgcgtcat ttaccagaaa
     1741 aaagtcccac caggcccatt tatcattgac gaccttaatc agtctgttca gggcacactg
     1801 gatgtcaaag tgacggaaga agatggtcgg gtgaacaatt tccaggtttc ggcagcatcg
     1861 acgcccttcc tgactcgtca gggacaggtt cgctataaac tggccgcggg tcagccacgg
     1921 ccctccatgt cacatcaaac tgaaaatgaa acctttttta gcaatgaagt ttcctagggg
     1981 atgctgtcaa acacctcgct gtacggcggc ctgctgcttt ctggtgatga ctaccattct
     2041 gccgcaatgg gtattgggca aaatatgctg tggcttggtg cgctgtcgtt tgatgtcacg
     2101 tgggccagta gccattttga tactcagcag gacgagcggg gcttaagcta ccgttttaat
     2161 tacagcaaac aagtggatgc cactaacagc acgatttccc tcgccgctta tcgtttctcc
     2221 gatcgtcatt ttcacagcta cgccaactat ctggatcaca aatacaacga cagcgatgcg
     2281 caggacgaaa aacagacgat cagcttatct gtgggccaac cgattacccc actaaacctc
     2341 aatctttacg ccaacctgct acatcaaacc tggtggaatg cagacgcctc cacgaccgcc
     2401 aacatcacgg caggttttaa tgttgatatt ggtgactgga gagatatctc tatttcgacg
     2461 tcattcaata caacccatta cgaagataaa gatcacgaca accagattta cctgtcgatt
     2521 tcgctcccct tcggtaacgg tggtcgggtt ggctatgaca tgcaaaacag tagccacagc
     2581 accacacacc gcatgtcgtg gaacgatacg ctggatgaac gtaatagctg gggcatgtct
     2641 gccggactgc aatccgaccg tcctgacaat ggagcccagg tgagcggcaa ctatcagcac
     2701 ctgagttcag cgggtgagtg ggatatttct ggtacctatg ccgccaatga ttacagttcc
     2761 gtcagcagca gctggagcgg ttctttcacc gcaacccaat atggtgcagc gtttcatcgc
     2821 cacagctcca ccaatgaacc acgcctgatg gtcagcaccg atggcgtggc agatattccg
     2881 gttcagggca atctcgacta caccaaccat tttggcattg cggtggtacc gttgatttcc
     2941 agttaccagc cttccaccgt ggcggtgaac atgaatgact tacccgacgg cgtaacagtt
     3001 gcagaaaacg ttatcaaaga aacgtggatt gaaggcgcga taggttacaa atcactggct
     3061 tcccgttccg gtaaagacgt taacgtcatc attcgcaacg ccagcggtca gtttcctccc
     3121 ctcggagcgg atatccgcca ggatgacagc ggcattagcg tggggatggt tggcgaggaa
     3181 ggacatgcct ggttaagcgg agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat
     3241 aaccagcatt gctcgctcca tcttcctgaa catatggaag acaccgcaaa tcgcctgatt
     3301 ttaccttgtc attaattaag gaaatgaaaa tgacatttat gaaaggacta ccccttttgt
     3361 tgttggttgc cagtctgtgc agccacgctg cactacaacc cgatcgcact cgtattgtgt
     3421 ttaacgctaa tgataaagct accagtctgc gggtggataa tcgtagcgat aaactcccct
     3481 acctcgctta ttcctggctc gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg
     3541 ccctgccgcc gatacaacgt ctggaaccga aagcaacaac gcaggtacgg atagtaaagc
     3601 aagcctcgac cacaaaatta ccgggcgatc gcgaaacgct atttttctac aatatgcgtg
     3661 aaattcctcc cgccccggaa aaaaacagcg accatgccgt tctgcaagtc gccattcaga
     3721 gtcgtatcaa ggtgttctgg cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg
     3781 aactgcagtt acaggtcagc cagcagggca accaactgac actgaaaaat cctaccgcgt
     3841 attatctgac cattgcctac ctgggacgaa atgaaaaagg cattctcccc ggcttcaaaa
     3901 ccgtgatggt tgcgcctttt agtacggtca acacaaacac cggaaattat agcggcagcc
     3961 agttttatct cggttatatg gatgattacg gcgcgttacg catgacgacg ctcaactgca
     4021 gcggacaatg ccatttacaa gccgtggagg cgaagaaatg agtgctggca agggagtgtt
     4081 gctcgtcatc tgtctgttat ttctgccgtt gaagtctgcg cttgccctga actgctactt
     4141 tggttcatcc ggtggctcgg tagaaaaatc agaagcgatt caaccttttg ctgtaccagg
     4201 caatgccaaa ctcggcgata agatttggga atctgacgat attaaaattc ccgtctattg
     4261 tgacaacaat accaacggca attttgaaag tgagcatgtt tatgcctggg taccccccta
     4321 cccgggggtg caggatcgct attatcaact cggcgtaact tacaatggcg ttgattatga
     4381 cgccaaccag ggaaaaagcc gcatcgacac taaccagtgt attgacagta agaacattga
     4441 catttatacc cctgagcaga ttatcgcgat gggatggcag aacaaaattt gctcgggtga
     4501 tcccgctaat attcacatgt cgcgtacctt tctcgctcgc atgcggttat acgtcaaaat
     4561 acgagaaatg ccgccgcatg attatcaaag tacgcttagc gactatatcg tcgtgcaatt
     4621 tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa aacctgaaat atcatattac
     4681 tggtctggaa aacattcgcg tgctggattg cagcgtcaat ttttccattt caccggaaac
     4741 acaggtgatt gattttggta aatttaattt gctggatatt cgtcgccaca caatgtcgaa
     4801 aacattcagt attaagacga caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt
     4861 cagttcctcc ttttataccg
//
LOCUS       DADLTD010000240         4862 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLTD010000240 DADLTD010000000
VERSION     DADLTD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.244"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            148..264
                     /locus_tag="JN701_003704"
     CDS             148..264
                     /locus_tag="JN701_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7366005.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            628..1470
                     /locus_tag="JN701_003705"
     CDS             628..1470
                     /locus_tag="JN701_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7366006.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1473..2561
                     /gene="capU"
                     /locus_tag="JN701_003706"
     CDS             1473..2561
                     /gene="capU"
                     /locus_tag="JN701_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7366007.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2566..3516
                     /gene="virK"
                     /locus_tag="JN701_003707"
     CDS             2566..3516
                     /gene="virK"
                     /locus_tag="JN701_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7366008.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
     gene            3581..4525
                     /gene="lpxM"
                     /locus_tag="JN701_003708"
                     /gene_synonym="msbB"
     CDS             3581..4525
                     /gene="lpxM"
                     /locus_tag="JN701_003708"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7366009.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(4679..>4862)
                     /locus_tag="JN701_003709"
     CDS             complement(4679..>4862)
                     /locus_tag="JN701_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY7366010.1"
                     /translation="IYQPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT         1386 a          923 c         1116 g         1437 t
ORIGIN      
        1 tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca ggattcagag
       61 tatgataaag acagattttc atggctgccg gtttccgtcg tgcaaaaaac atgctgattt
      121 tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat cagatggaac
      181 aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg ccgggagggc
      241 gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat agaagttcgt
      301 gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt cctttttttc
      361 attggagcac ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt agccaaattc
      421 agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa tatatcattg
      481 tttatgaatt gttgatgttt aatttagtaa aatatatcaa gtatgccatg ggtttgctat
      541 gttattcatc cagtatctgg aaataaaatg caccagcttt attgggtttt ccttttgtct
      601 ctgcttacta ggtgccagaa actttaaatg ttaaacgaag ggggcatttt gttcaaagcc
      661 aatcatgttc cggtattaat gtatcatcat gtcagtcatt gccccggact ggttacactt
      721 tctcccgtga cattccggaa gcaaatgaaa tggttggctg aaaataactg gaaaacactg
      781 tcatcagatg aactggagtt tttttatcgg ggcggaaaac tcccccggaa aagcgttatg
      841 ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt attaaaagag
      901 tttaatctca aagcacatat ttttttaatt accggtttta ttgggaatgg tccggttcgt
      961 cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc gacagggaat
     1021 gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc
     1081 gaatttcatg ttcatacgca tactcataca cgctgggata aaaagttcag ttcacgagaa
     1141 gaacagtgta aacatcttcg gcaggacttg ttgagtggga gagaatattt gaaagaaatg
     1201 acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataacaa ggattatatt
     1261 caggtcgctg aagaacttgg attctattat ttatatacaa cagaaagaag aatgaatgct
     1321 cctgctaaag gaactacacg gataggaaga ataagcacca aagaacggga aagttgcgca
     1381 tggctgaaaa gacgactttt ttattataca acaccgtttt tttcatcgtt gctggccttt
     1441 cataaggggc caagattacc ggatgattaa taatgaatat actatttacg gaatcatcac
     1501 cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg
     1561 ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat
     1621 tgggaattga tatcacattc gcgttatttc gaaacagtct tcacatccct actgcatgga
     1681 gattactcgg aatagttcat ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg
     1741 atagcaatat tgttggttta gtacggttat tcacttggaa acatccattc agaattatca
     1801 ggcaaaagac atatttgaca caaaaaacaa aagttttttc aataaatcat ttttgcgatg
     1861 aggtgattgt tcccggaaca agtatgaaga cacatctgga gcaggaagga tgtcgaaccc
     1921 gggttactgt tgtgcctcca ggctttgatt tccagaaatt atacgttgat tcccgaaaca
     1981 gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag gggatgcccc gttattgctc
     2041 aggtaggaat gttgcgcccg gaaaaagggc acgaatttat gttgaattta ctgttccatt
     2101 taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttcg cctgaactgc
     2161 gggagcattt acagtatcag attgacagta tgggcatgca tgatgatgtt tttattgctg
     2221 acaatgtttt tcctgccgcc cccgtatatc gggttgccag tctggtggtt ctgccttcag
     2281 aaaacgaatc gtttggtatg gtactggcag aagcatcggc attttctgtg cctgtactgg
     2341 ccagtcagat tggtggaatc cctgatgtta ttcagaacaa ccagaccggg acattgttac
     2401 cagcaggtaa taagcacgca tggatgtgcg ccctgaatga tttttttaat gaccctgggc
     2461 gtttttatca gatggctcgc caggcaaaac aggatataga agagcggttt gatattaata
     2521 aaactgcgtt aaaaatactc acattagcga agcacaagta acaatatgtt ttctgtaagt
     2581 aacttatcat ttatcggttt ccttaaaagg attgtttttt cctcagattc actcccgggg
     2641 aagtgggaac acagaaaatt ccggttcatg tacattttgc gatgtgctat aaatccggtt
     2701 gccagtattc gatattatta cgaactgcgt tccttgccgt gcattgagga tattctggca
     2761 atacacccca cgttgccagc aagaattcat cgcccttatt tgcataaagg ggggcgcgcc
     2821 tggacccgtg gacaatatat tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa
     2881 tattctaaat ttttgtttcc acaaaaatcg gtatcattag tccaattcat cgggaaagat
     2941 ggagagaatt ttgatatcca gtgttcttcc agtggttttg accgggaagg agagttgatg
     3001 ttgtcattgt tttacaataa aacagttatt acccggttaa cattttcagt cattctgact
     3061 caaaatggac acattgcttt tattggcgga ttacagggag cccctaaaaa taccggacct
     3121 gatgttattc ggtgcgcaac ccgggcctgt tatgggattt tcccaaaacg gattattttt
     3181 gaagcatttt gtgcgttgat gaaagcttgc aatatctctg agtgtctggc agtcagtgag
     3241 cacagtcatg ttttccggca attgagatac tggtatcaga aacgcaaaac ctttgttgcg
     3301 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
     3361 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgagtac
     3421 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
     3481 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
     3541 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
     3601 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggtcta catggttcct
     3661 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
     3721 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
     3781 tttatcgctt tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat
     3841 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
     3901 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcgatacaa
     3961 taacagaaat gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt
     4021 gctggcagct cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat
     4081 tgattatgtc tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga
     4141 tgggataaaa ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga
     4201 tcaggatcat ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac
     4261 attaccaatt attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc
     4321 ggtttatgat gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg
     4381 cattgccagc gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt
     4441 ggtggggtca catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc
     4501 aaacgaagcg gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc
     4561 ttacaggtta ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga
     4621 ctgtgtagat gcagttcgtt aaatttaatt tatgcctaaa tagctgcgct taataccgct
     4681 acacttttgc cagtccatgt ttgcctccgg ggaatgggct gacggtttcc ataaaatggc
     4741 gaactttttt caacagttgc cacattgagc ggcactgatg attacgcgtt attgtgtcgt
     4801 gaagtgcctg ccatagccgt tcagcatgat tcacccatgg cgagtaaacc ggctgataaa
     4861 tg
//
LOCUS       DADLTD010000241         4835 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.245, whole genome shotgun sequence.
ACCESSION   DADLTD010000241 DADLTD010000000
VERSION     DADLTD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.245"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(64..511)
                     /locus_tag="JN701_003710"
                     /pseudo
     CDS             complement(64..511)
                     /locus_tag="JN701_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            668..1597
                     /gene="metA"
                     /locus_tag="JN701_003711"
     CDS             668..1597
                     /gene="metA"
                     /locus_tag="JN701_003711"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY7366011.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1866..3467
                     /gene="aceB"
                     /locus_tag="JN701_003712"
     CDS             1866..3467
                     /gene="aceB"
                     /locus_tag="JN701_003712"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY7366012.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            3497..4794
                     /gene="aceA"
                     /locus_tag="JN701_003713"
                     /pseudo
     CDS             3497..4794
                     /gene="aceA"
                     /locus_tag="JN701_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1212 a         1256 c         1227 g         1140 t
ORIGIN      
        1 aaaaaatgcc gggtgcgacg ctggcgcgtc ttctccagcc tgggcaggca cggcaggggc
       61 ggcttattcc cccacatacg ccagattcag caacggatac ggtttcccca aatcgtccac
      121 ctcagagcgt cccgtaacct taaaacccac cttcttatag aacccaaccg cctgctcatt
      181 ttgctcatta acgttggttg tcagttccgg tgccatcgag agcgcatgct ccaccagcat
      241 ccgacccacg ccgcagccgc acacatcagg atcgataaac agcgcatcca tatgctgccc
      301 acttaacaac ataaacccaa ccggctgatc ccgttcatta accgcgatcc acaacggcgc
      361 ttccggcagg aaggaacgaa ctagctagca cctccagctc ggcccgatac tctgctgata
      421 aaaaatcgtg tgtggcatcg acagatcgac accaaatcgc aacgagttcc tccccttcct
      481 catgccgtga gcggcgaata ctaataacca ttttctctcc ttttagtcat tcttatattc
      541 taacgtagtc ttttccttga aactttctca ccttcaacat gcaggctcga cattggcaaa
      601 ttttctggtt atcttcagct atctggatgt ctaaacgtat aagtgtatgt agtgaggtaa
      661 tcaggttatg ccgattcgtg tgccggacga gctacccgcc gtcaatttct tgcgtgaaga
      721 aaacgtcttt gtgatgacaa cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt
      781 tctgatcctt aacctgatgc cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct
      841 ttcaaactca cctttgcagg tcgatattca gctgttgcgc atcgattccc gtgaatcgcg
      901 caacacgccc gcagagcatc tgaacaactt ctactgtaac tttgaagata ttcaggaaca
      961 gaactttgac ggtctgattg taaccggtgc gccgctgggc ctggtggagt ttaatgatgt
     1021 cgcttactgg ccgcagatca aacaggtgct ggagtggtcg aaagatcacg tcacctcgac
     1081 gctgtttgtc tgctgggcgg tacaggccgc gctcaatatc ctctacggca ttcctaagca
     1141 aactcgcacc gacaaactct ctggcgttta cgagcatcat attctccatc ctcatgcgct
     1201 tctgacgcgt ggctttgatg attcattcct ggcaccacat tcgcgctatg ctgactttcc
     1261 ggcagcgttg attcgtgatt acaccgatct ggaaattctg gcagagacgg aagaagggga
     1321 tgcatatctg tttgccagca aagataagcg cattgccttt gtgacgggcc atcccgaata
     1381 tgatgcgcaa acgctggcgc aggaatattt ccgcgatgtg gaagccggac tagacccgga
     1441 agtaccgtat aactatttcc cgcacaatga tccgcaaaat acaccgcgag cgagctggcg
     1501 tagtcacggt aatttgctgt ttaccaactg gctcaactat tacgtctatc agatcacgcc
     1561 atacgatcta cggcacatga atccaacgct ggattaatct tctgtgatag tcgatcgtta
     1621 agcgattcag caccttacct caggcacctt cgggtgcctt ttttatttcc gaaacgcacc
     1681 tcaggaggtg aataaatttt attcacattg ttatcaataa gttatcaagc attttcaatt
     1741 aaaatggaaa ttgtttttga ttttgcattt taaatgagta gtcttagttg tgctgaacga
     1801 aaagcgcaca acgatccttc gttcacagtg gggaagtttt cggatccatg acgaggagct
     1861 gcacgatgac tgaacaggca acaacaaccg atgaactggc tttcacaagg ccgtatggcg
     1921 agcaggagaa gcaaattctt actgccgaag cggtagaatt tctgactgag ctggtgacgc
     1981 attttacgcc acaacgaaat aaacttctgg cagcgcgcat tcagcagcag caagatattg
     2041 ataacggaac gttgcctgat tttatttcgg aaacagcttc catttgcgat gctgattgga
     2101 aaattcgcgg gattcctgcg gacttagaag accgccgcgt agagataact ggcccggtag
     2161 agcgcaagat ggtgatcaac gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg
     2221 aagattcact ggccccggac tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg
     2281 cagttaacgg taccatcagc tataccaatg aagcaggcaa aatttaccag ctcaagccca
     2341 atccagcggt tttgatttgt cgggtacgcg gtctgcactt gccggaaaaa catgtcacct
     2401 ggcgtggtga ggcaatcccc ggcagcctgt ttgattttgc gctctatttc ttccacaact
     2461 atcaggctct gttggcaaag ggcagtggtc cctatttcta tctgccgaaa acccagtcct
     2521 ggcaggaagc ggcctggtgg agcgaagtct tcagctatgc agaagatcgc tttaatctgc
     2581 cgcgcggcac catcaaggcg acgttgctga ttgaaacgct gcccgccgtg ttccagatgg
     2641 atgaaatcct tcacgcgctg cgtgaccata ttgttggtct gaactgcggt cgttgggatt
     2701 acatcttcag ctatatcaaa acgttgaaaa actatcccga tcgcgtcctg ccagacagac
     2761 aggcagtgac gatggataaa ccattcctga atgcttactc acgcctgttg attaaaacct
     2821 gccataaacg cggtgctttt gcgatgggcg gcatggcggc gtttattccg agcaaagatg
     2881 aagagcacaa taaccaggtg ctcaacaaag taaaagcgga taaatcgctg gaagccaata
     2941 acggtcacga tggcacatgg atcgctcacc caggccttgc ggatacggca atggcggtat
     3001 tcaacgacat tctcggctcc cgtaaaaatc agcttgaagt gatgcgcgaa caagacgcgc
     3061 cgattactgc cgatcagcta ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc
     3121 gcgcaaatat tcgcgtagcc gtgcagtaca tcgaagcgtg gatctctggc aacggctgtg
     3181 tgccgattta tggcctgatg gaagatgcgg cgacggctga aatttcccgt acctcaatct
     3241 ggcagtggat ccatcatcaa aaaatgttga gcaatggcaa accggtaact aaagccttgt
     3301 tccgccagat gctgggcgaa gagatgaaag tcattgccag cgaactgggc gaagaacgtt
     3361 tctcccaggg gcgttttggc gatgccgcac gcttgatgga acagatcacc acttccgatg
     3421 agttaattga tttcctgacc ctgccaggct actgcctgtt agcgtaaacc accacataac
     3481 tatggagcat ctgcacatga aaacccgtac acaacaaatt gaagaattac agaaagagtg
     3541 gactcaaccg cgttgggaag gcattactcg cccatacagc gcggaagatg tggtgaaatt
     3601 acgcgattca gtcaatcctg aatgcaccct ggcgcaactg ggcgcggcga gaatgtggcg
     3661 tctgctgcat ggcgagtcga aaaaagctac atcaacagcc tcggtgcact gactggcggt
     3721 caggcgctgc aacaggcgaa agcgggtatt gaagcagtct atctgtcggg atggcaggta
     3781 gcggcggacg ctaacctggc ggccagcatg tatccggatc agtcgctcta tccggcaaac
     3841 tcggtgccag ctgtggtgga gcggatcaac aatactttcc gccgtgcgga tcagatccaa
     3901 tggtccgcgg gcattgagcc gggcgatccg cgctatgtcg attacttcct gccgatcgtt
     3961 gccgatgcgg aagccggttt tggcggtgtc ctgaatgcct ttgaactgat gaaagcgatg
     4021 attgaagccg gtgcagcggc agttcacttc gaagatcagc tggcgtcagt gaagaaatgc
     4081 ggtcacatgg gcggcaaagt tttagtgcca actcaagaag ctattcagaa actggtcgcg
     4141 gcgtgtctgg cagctgacgt gacgggcgtt ccaaccctac tggttgctcg taccgatgcc
     4201 gcggatctga tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt
     4261 accagtgaag gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg
     4321 gcgtatgcgc catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg
     4381 gcgcgtcgct ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac
     4441 tgctcgccgt cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag
     4501 cagcagctgt cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc
     4561 atgtggttca acatgtttga cctggcaaac gcctatgacc agggcgaggg tatgaagcac
     4621 tacgttgaga aagtgcagca gccggaattt gccgccgcga aagatggcta taccttcgta
     4681 tctcaccagc aggaagtggg tacaggttac ttcgataaag tgacgactat tattcagggc
     4741 ggcacgtctt cagtcaccgc gctgaccggc tccactgaag aatcgcagtt ctaagcaaca
     4801 acaaccgttg ctgactgtag gccggataag gcgtt
//
LOCUS       DADLTD010000242         4829 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLTD010000242 DADLTD010000000
VERSION     DADLTD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.246"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..836
                     /locus_tag="JN701_003714"
     CDS             <1..836
                     /locus_tag="JN701_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7366013.1"
                     /translation="TDVWKYRGKSTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGST
                     KYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRR
                     ITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVS
                     AGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQ
                     AYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQ
                     W"
     gene            1204..2859
                     /gene="lldP"
                     /locus_tag="JN701_003715"
     CDS             1204..2859
                     /gene="lldP"
                     /locus_tag="JN701_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY7366014.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            2859..3635
                     /gene="lldR"
                     /locus_tag="JN701_003716"
     CDS             2859..3635
                     /gene="lldR"
                     /locus_tag="JN701_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY7366015.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            3632..4822
                     /gene="lldD"
                     /locus_tag="JN701_003717"
     CDS             3632..4822
                     /gene="lldD"
                     /locus_tag="JN701_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY7366016.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1068 a         1279 c         1308 g         1174 t
ORIGIN      
        1 taacagatgt ctggaaatat aggggcaaat ccacccagag cattactaac ctgaacaatc
       61 aggtcactaa tctggatact cgcgttacta atatcgaaaa cggtattggc gacattgtca
      121 ccaccggtag caccaagtac ttcaagacca acaccgatgg cgtagatgcc aacgcccagg
      181 gtaaagatag cgttgctatt ggttctggtt ccattgctgc cgctgacaac agcgtcgcac
      241 tgggtaccgg ttccgttgca gaggaagaaa atacaatctc tgtaggttct tctactaacc
      301 aacgccgtat caccaacgta gctgccggtg ttaatgccac cgatgcggtt aacgtgtcgc
      361 agctgaaatc ttctgaagca ggcggcgtgc gctacgacac caaagctgat ggttctatcg
      421 actatagcaa tatcaccctc ggtggtggca acggcagtac gactcgtatc agcaacgtct
      481 ccgctggcgt caacaacaac gacgcggtga actacgcgca gttgaagcaa agcgtgcagg
      541 aaacgaagca atacaccgat cagcggatgg ttgagatgga taacaaactg tctaaaactg
      601 aaagcaagtt gagcggtggt atcgcttctg caatggcaat gaccggtctg ccgcaggctt
      661 atacaccggg tgccagcatg gcttctattg gtggcggtac ttacaacggt gaatcggcag
      721 ttgctttagg tgtatcgatg gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta
      781 gtaccaatag ccagggtgaa tactccgccg cactcggtgc cggtattcag tggtaatgat
      841 ccattaagtt agtgtgacta agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag
      901 gcaagataag gcgctccggg tcaacaatct aagggcaatt ctctgatgag gattgccctt
      961 ttctttacca gacatctccc cccacaagaa ttggccctac caattcttcg cttatctgac
     1021 ctctggttca caattcccca attaaaactc acatcaatat tgccaataca caacatttag
     1081 ttaaccattc attgtcatta tccctacaca acacaattgg cagtgccact tttacacaac
     1141 gtgtgacaag gagatgagca acagactcat tacacgatgt gcgtggactc caggagacct
     1201 gcaatgaatc tctggcaaca aaactacgat cccgccggga atatctggct ttccagtctg
     1261 atagcatcgc ttcccatcct gtttttcttc tttgcgctga ttaagctcaa actgaaagga
     1321 tacgtcgccg cctcatggac ggtagcaatc gcccttgccg tggctttgct gttctataaa
     1381 atgccggtcg ctaacgcgct ggcctcggtg gtttatggct tcttctatgg tttgtggccc
     1441 atcgcgtgga tcattattgc agcagtgttc gtctataaga tctcggtgaa aaccgggcag
     1501 tttgacatca ttcgctcgtc tattctttcg ataacccctg accagcgtct gcaaatgctg
     1561 atcgtcggtt tctgtttcgg cgctttcctt gaaggagccg caggctttgg cgcaccggta
     1621 gcaattaccg ccgcattgct ggtcggcctg ggttttaaac cgctgtacgc cgccggactg
     1681 tgcctgattg ttaacaccgc gccagtggca tttggcgcga tgggcattcc aatcctggtt
     1741 gccggacaag taacaggcat cgacagtttt gagattggcc agatggtggg gcgtcagcta
     1801 ccgtttatga ccattatcgt gctgttctgg atcatggcga ttatggacgg ctggcgcggt
     1861 atcaaagaga cgtggcctgc ggtcgtggtt gcgggcggct cgtttgccat cgctcagtac
     1921 cttagctcca acttcattgg gccggagctg ccggacatta tctcttcgct ggtatcactg
     1981 ctctgcctga cgctgttcct caaacgctgg caaccagttc gcgtattccg cttcggtgac
     2041 ttgggggcgt cacaggttga tatgacgctg gcccacaccg gttacaccgc gggtcaggtg
     2101 ttacgtgcct ggacaccgtt cctgttcctg accgctaccg taacactgtg gagcatcccg
     2161 ccgtttaaag ccctgttcgc atcgggtggt gcgctgtatg agtgggtgat caacattccg
     2221 gtgccgtacc tcgataaact ggttgcccgt atgccaccag tggtcagcga ggctacagcc
     2281 tatgccgccg tgtttaagtt tgactggttc tctgccaccg gcaccgccat tctgtttgct
     2341 gcactgctct cgattgtctg gctgaagatg aaaccgtctg acgctatcag caccttcggc
     2401 agcacgctga aagaactggc tctacccatc tactccatcg gcatggtgct ggcgttcgcc
     2461 tttatctcga actattccgg actgtcatca acactggcac tggcactggc gcacaccggt
     2521 catgcattca ccttcttctc gccgttcctc ggctggctgg gggtattcct gaccgggtcg
     2581 gatacctcat ctaacgccct gttcgccgcg ctgcaagcca ccgcagcaca acaaattggc
     2641 gtctctgatc tgttgctggt tgccgccaat accaccggtg gcgtcaccgg taagatgatc
     2701 tccccgcaat ctatcgctat cgcctgtgcg gcggtaggcc tggtgggcaa agagtctgat
     2761 ttgttccgct ttactgtcaa acacagcctg atcttcacct gtatggtggg cgtgatcacc
     2821 acgcttcagg cttatgtctt aacgtggatg attccttaat gattgtttta cccagacgcc
     2881 tgtcagacga ggttgccgat cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg
     2941 gcatgaagtt gcccgctgag cgccaactgg cgatgcaact cggcgtatca cgtaattcac
     3001 tacgcgaggc gctggcaaaa ctagtgagcg aaggcgtgct gctcagtcga cgcggcggcg
     3061 ggacgtttat tcgctggcgt catgacacat ggtcggagca aaacatcgtc cagccgctaa
     3121 aaacgctgat ggccgatgat ccggattaca gtttcgatat tctggaagcc cgctacgcca
     3181 ttgaagccag caccgcatgg catgcggcaa tgcgcgccac acctggcgac aaagaaaaga
     3241 ttcagctttg ctttgaagca acgctaagtg aagacccgga tatcgcctca caagcggacg
     3301 ttcgttttca tctggcgatt gccgaagcct cacataacat cgtgctgctg caaaccatgc
     3361 gcggtttctt cgatgtcctg caatcctcag tgaagcatag ccgtcagcgg atgtatctgg
     3421 tgccaccggt tttttcacaa ctgaccgaac aacatcaggc tgtcattgac gccatttttg
     3481 ctggtgatgc cgacggggcg cgtaaagcaa tgatggcgca ccttagtttt gttcacacca
     3541 ccatgaaacg attcgatgaa gatcaggctc gccacgcacg gattacccgc ctgcccggtg
     3601 agcataatga gcattcgagg gagaaaaacg catgattatt tccgcagcca gcgattatcg
     3661 cgccgcagcg caacgcattc tgccgccgtt cctgttccac tatatggatg gtggtgcata
     3721 ttctgaatac acgctgcgcc gcaacgtgga agatttgtca gaagtggcac tgcgccagcg
     3781 tattctgaaa aacatgtccg acttaagcct ggaaacgacg ctgtttaatg agaaattgtc
     3841 gatgccggta gcactggcac cggtgggttt gtgtggcatg tatgcgcgtc gtggcgaagt
     3901 tcaggcagcc aaagcggcgg acacgcatgg cattccgttt actctctcga cggtttccgt
     3961 ttgcccgatt gaagaagtcg cgccagccat caagcgccca atgtggttcc agctttatgt
     4021 actgcgcgat cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc
     4081 gacgctggtt ttcaccgtgg atatgccgac accgggcgca cgctaccgtg atgcgcattc
     4141 aggtatgagc ggcccaaatg cggcaatgcg ccgctacttg caggcggtga cgcatccgca
     4201 atgggcgtgg gatgtgggcc tgaacggtcg tccacatgat ttaggtaata tctcagctta
     4261 tctcggcaaa ccaaccggac tggaagatta catcggctgg ctggggaata acttcgatcc
     4321 gtccatctca tggaaagacc ttgagtggat ccgcgatttc tgggatggcc cgatggtgat
     4381 caaagggatc ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg ctgatggaat
     4441 tgtggtttct aaccacggtg gccgccagct ggacggtgta ctctcttccg ctcgtgcgtt
     4501 gcccgctatt gcagatgcgg tgaaaggtga tatcgccatt ctggcggata gcggaattcg
     4561 taacgggctt gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg
     4621 tgctttcctg tatgcgctgg caacagcggg ccaggcgggt gtagctaacc tgctaaatct
     4681 gatcgaaaaa gagatgaaag tggcgatgac gctgactggc gcgaaatcga tcagtgaaat
     4741 tacgcaagat tcgctggtgc aagggctggg taaagagttg cctgcggcac tggctcccat
     4801 ggcgaaaggg aatgcggcat agtcgtttg
//
LOCUS       DADLTD010000243         4721 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLTD010000243 DADLTD010000000
VERSION     DADLTD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.247"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            70..255
                     /locus_tag="JN701_003718"
                     /pseudo
     CDS             70..255
                     /locus_tag="JN701_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            398..619
                     /locus_tag="JN701_003719"
                     /pseudo
     CDS             398..619
                     /locus_tag="JN701_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(640..702)
                     /locus_tag="JN701_003720"
                     /pseudo
     CDS             complement(640..702)
                     /locus_tag="JN701_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1153..2565
                     /gene="ospC1"
                     /locus_tag="JN701_003721"
     CDS             1153..2565
                     /gene="ospC1"
                     /locus_tag="JN701_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY7366017.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2669..2817)
                     /locus_tag="JN701_003722"
                     /pseudo
     CDS             complement(2669..2817)
                     /locus_tag="JN701_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2893..4590
                     /gene="ospD3"
                     /locus_tag="JN701_003723"
     CDS             2893..4590
                     /gene="ospD3"
                     /locus_tag="JN701_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY7366018.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1645 a          853 c          877 g         1346 t
ORIGIN      
        1 gtttatcctg tttttataac ggtaattgca gaccatgaca aaccgcagcc gtcaggctgc
       61 ctactcgagt cacaaggctc tctgtgtccg atatgccggc agcgcattac ccatgaaact
      121 ggctggaacg tacaccataa ggtgaagaaa gttatggggg gcggtgaaga attatctaac
      181 cttgtcttgt tacatccaaa ttgccatcga cagttacaca gttgtgaagc cggctctctc
      241 aaaaagggct tataaaggct tgagcggtgt accgggaaac cggtatgcac cgttcttagg
      301 ggagggcagt ccagcaatga actgtcctta cccgacaaac atccaaaacg aacggaacgt
      361 gctggaatcc gcatataacc cgttgtaaag tgaggaaatg gacacctcac ttgctcatga
      421 gaacgcccgc ctgcgggcac tgttgcagac gcaacaggac actatccacc agatggctga
      481 atacaaccgc ctgctctcac agcggatggc ggcttatgct tccgaaatca accgactgaa
      541 ggcgctggtt gcgaaactgc agcgtatgca gttcggtaaa agcccagaaa aacttcgcgc
      601 aaaaaccgaa cggcagattc cattttcacg ggcgatctac gccacacagg ctctgggttg
      661 cagtgactgc tccattaagg cgattttgaa ctcataaggg aagtaacaaa aaaactgcaa
      721 aagtacaata attatatatg gccaaactaa cctctattaa aaaaacatca gtttttcaca
      781 cttagttgag cttcatgaaa aaatcatcga ggatttcatt gcagaataca cattccacta
      841 actggaagca ctccataaga ggaatcagag aaagtatcgt ttttttatag taaaattcat
      901 tgctgttcaa taaaattgat ataattatta aattatggaa ataatttcac aaaaaagatt
      961 aacttgagtt cttaagcatc ggattttaga tattttgaaa tatctggcat cacataacat
     1021 caaaagtata taataccctt atctaattac atgcagtata ttgtatatat actcaaaaat
     1081 tgtaattcat accctactat atcttgtggg gaaagatatt aattaaaact gttttcatat
     1141 aaggttcatt ttatgaatat atcagaaaca ctgaactcag caaataccca atgcaatata
     1201 gattctatgg ataacagatt acatacattg tttccaaaag tgacatcagt gcgaaacgct
     1261 gcacaacaaa ctatgccaga tgaaaaaaat ttaaaagata gtgcaaatat tattaaagat
     1321 ttctttagga aaactatagc agcacaaagt tatagtagaa tgttctctca aggctctaac
     1381 tttaaatctt taaatatagc aattgatgca ccatcagacg ctaaagcctc atttaaggct
     1441 attgagcacc ttgacagatt atcgaagcat tatatatctg aaataaggga aaaacttcat
     1501 cctctttctg cagaggaact caatttgctt tcgctaatta ttaattctga tttaatcttc
     1561 agacatcaaa gtaattctga tttgtctgat aaaattttaa acattaagtc attcaataaa
     1621 attcagtctg aaggaatatg cacaaaacga aacacatacg ctgatgatat aaaaaaaata
     1681 gctaatcatg actttgtgtt ttttggcgtt gaaatctcta gccatcagaa aaaacacccc
     1741 ctgaatacaa aacatcacac tgttgatttt ggtgcaaatg cgtatatcat tgatcatgac
     1801 tctccatatg gatatatgac attaaccgat cactttgata atgctattcc acctgttttt
     1861 taccatgagc accaatcatt tttagataaa ttttcagagg ttaataaaga agttagtcga
     1921 tacgtacatg gaagtaaagg aattatagat gtaccaatat tcaatactaa agatatgaag
     1981 ttagggctcg gattatacct gattgacttt attagaaaaa gtgaagacca aagcttcaag
     2041 gagttttgct atggaaaaaa tcttgcccct gtggatctgg atagaatcat aaactttgtt
     2101 tttcagccag agtaccatat acctaggatg gtaagtacag aaaacttcaa aaaagttaag
     2161 attagagaaa tatccttaga ggaggctgtt acagcatcta attacgaaga aattaacaag
     2221 caggtcacta acaaaaaaat tgctctccag actctttttc tttcgattac taatcaaaaa
     2281 gaggatgtcg ccttatatat attatctaat tttgagataa ctagacaaga tgttatttcc
     2341 ataaagcatg agttgtatga tattgagtat ctacttagcg ctcataattc aagctgtaaa
     2401 gtacttgagt attttatcaa taagggattg gttgatgtaa acacaaagtt caaaaaaact
     2461 aatagtgggg attgtatgtt ggataacgca ataaaatatg agaatgcaga aatgataaaa
     2521 ctattattga aatatggtgc aacatctgac aataaatata tttaatcaaa attgaatatc
     2581 gtttagcaaa aggcagtatt tgcacaaaat atcgatatat tgtttattgt cagtatggct
     2641 caatgtgata atagttggaa agtttgatgg gtttcgcccc gttgtagcgg tagtcgagct
     2701 ggaaggtctt caggcactgc ttacagcgat agagcagccc ccctgaactg gaatggccgt
     2761 tccggatacc ccctgagttt cagagtaacg gggacaaacc acatcaatct ttgccatcaa
     2821 tcatccaaag ggcaaagagt acaacaacac taagtctgcg tcacaaccca tcaatgaaag
     2881 gaatatatac atatgccatc agtaaattta atcccatcaa ggaaaatatg tttgcaaaat
     2941 atgataaata aagacaacgt ctctgttgag acaatccagt ctctattgca ctcaaaacaa
     3001 ttgccatatt tttctgacaa gaggagtttt ttattaaatc taaattgcca agttaccgat
     3061 cactctggaa aacttattgt ctgtcgacac ttagcttcct actggatagc acagtttaac
     3121 aaaagtagtg gtcacgtgga ttatcatcac tttgcttttc cggatgaaat taaaaattat
     3181 gtttcagtga gtgaagaaga aaaggctatt aatgtgcctg ctattattta ttttgttgaa
     3241 aacggttcat ggggagatat tattttttat attttcaatg aaatgatttt tcattccgaa
     3301 aaaagcagag cactagaaat aagtacatca aatcacaata tggcattagg cttgaagatt
     3361 aaagaaacta aaaatggggg ggattttgtc attcagcttt atgatcccaa ccatacagca
     3421 actcatttac gagcagagtt taacaaattt aacttagcta aaataaaaaa actgactgta
     3481 gataattttc ttgatgaaaa acatcagaaa tgttatggtc ttatatccga cggtatgtct
     3541 atatttgtgg acagacatac tccaacaagc atgtcctcca taatcagatg gcctaataat
     3601 ttacttcacc ccaaagttat ttatcacgcg atgcgtatgg gattgactga gctaatccaa
     3661 aaagtaacaa gagtcgtaca actatctgac ctttcagaca atacgttaga attacttttg
     3721 gcagccaaaa atgacgatgg tttgtcagga ttgcttttag ctttacaaaa tgggcattca
     3781 gatacaatct tagcatacgg agaactcctg gaaacttctg gacttaacct tgataaaacg
     3841 gtagaactac taactgcgga aggaatggga ggacgaatat cgggtttatc ccaagcactt
     3901 caaaatgggc atgcagaaac tatcaaaaca tacggaaggc ttctcaagaa gagagcaata
     3961 aatatcgaat acaataagct gaaaaattta ctgaccgctt attattatga tgaagtacac
     4021 agacagatac ccggactaat gtttgctctt caaaatggac atgcagatgc tatacgcgca
     4081 tacggtgagc tcattcttag cccccctctc ctcaactcag aggatattgt aaatttgctg
     4141 gcctcaagga gatatgacaa tgttcccgga cttctgttag cattgaataa tggacaggct
     4201 gatgcaatct tagcttatgg tgatatcttg aatgaggcaa aacttaactt ggataaaaaa
     4261 gcagagctgt tagaagcgaa agattctaat ggtttatctg gattgtttgt agccttgcat
     4321 aatggatgtg tagaaacaat tattgcttat gggaaaatac ttcacactgc agaccttact
     4381 ccacatcagg catcaaaatt actggcggca gaaggcccaa atggggtatc tggattaatt
     4441 atagcttttc aaaataggaa ttttgaggca ataaaaactt atatgggaat aataaaaaat
     4501 gaaaatatta cacctgaaga aatagcagaa cacttggaca aaaaaaatgg aagtgatttt
     4561 ctagaaatta tgaagaatat aaaaagctga atattatctc gtggtgatac actgaatttg
     4621 aggtaaccgg ctcatttaaa ccgtctggtc tgttacctcc ggttccacaa aaataatgtc
     4681 catcattttg aagtggacac tatcgtatgg aataccggac c
//
LOCUS       DADLTD010000244         4673 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLTD010000244 DADLTD010000000
VERSION     DADLTD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.248"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            60..1859
                     /gene="cysJ"
                     /locus_tag="JN701_003724"
     CDS             60..1859
                     /gene="cysJ"
                     /locus_tag="JN701_003724"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY7366019.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1859..3571
                     /gene="cysI"
                     /locus_tag="JN701_003725"
     CDS             1859..3571
                     /gene="cysI"
                     /locus_tag="JN701_003725"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY7366020.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3646..4380
                     /gene="cysH"
                     /locus_tag="JN701_003726"
     CDS             3646..4380
                     /gene="cysH"
                     /locus_tag="JN701_003726"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7366021.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1153 a         1217 c         1334 g          969 t
ORIGIN      
        1 tgttttatgg gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca
       61 tgacgacaca ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc
      121 ttcaggcggc cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct
      181 ggggcgtact caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa
      241 tgccgggtat aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag
      301 cattacgcga tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact
      361 ataaattcaa acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg
      421 aaggggaacc gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc
      481 caaagctgga aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt
      541 tctgccagtc cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc
      601 tcgaccgtgt cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg
      661 tggttgatgc gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg
      721 gcgcggtaaa tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc
      781 tctctgttaa ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa
      841 ttgacttagg tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc
      901 agaacgatcc ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac
      961 ctgtcaccgt cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac
     1021 tgaccgtcaa caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac
     1081 tgctgccgct ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg
     1141 tcgacatggt gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc
     1201 gcccgctgac cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag
     1261 tacacgtcac cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg
     1321 cctccagctt cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac
     1381 ataacgataa ctttcgcctg cccgctaacc cggaaacccc ggtgattatg attggcccag
     1441 gcaccggtat tgcgccgttc cgcgccttta tgcagcaacg cgccgccgac gaagcgccag
     1501 gtaaaaactg gctgttcttt ggtaatccgc actttacgga agacttcctg taccaggtgg
     1561 agtggcagcg ctacgtcaaa gatggcgtgc tgacacgtat cgatcttgcc tggtcgcgcg
     1621 accaaaaaga aaaagtttac gtacaagaca aactgcgcga acagggcgcg gagctgtggc
     1681 gctggatcaa tgatggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag
     1741 acgttgagca ggcacttctg gaagtgattg ccgaatttgg tggcatggac atcgaagcgg
     1801 cggatgaatt tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat
     1861 gagcgaaaaa catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat
     1921 gaagcttgaa agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac
     1981 cggcggcttt aagggcgaca acttcctgct gatccgcttc cacggcatgt atcagcagga
     2041 tgaccgcgac atccgcgccg aacgtgctga acagaagctg gagccgcacc acgcgatgct
     2101 gcttcgctgt cgtctgccgg gtggggtgat taccactaaa cagtggcagg cgatcgacaa
     2161 atttgccggt gaaaacacca tctatggcag cattcgcctg accaaccgcc agacattcca
     2221 gttccacggc attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg
     2281 tcttgatgcg ctggcgacag ctaacgacat gaaccgtaac gtactctgca cctcgaaccc
     2341 ttacgagtcg cagctgcacg cggaagcgta cgagtgggcg aagaagattt ctgagcatct
     2401 gttgcctcgt acccgcgcgt atgcggagat ctggctcgac caggaaaaag tcgccactac
     2461 tgatgaagaa ccgatccttg gtcagaccta cctgccgcgt aagttcaaaa ccacggtagt
     2521 gatcccgccg cagaacgata tcgatctgca tgccaacgac atgaacttcg tggcaatcgc
     2581 cgaaaacggc aagctggtag gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca
     2641 cggcaacaag aaaacctacg cccgcacggc gagcgagttt ggctatctgc cgctggagca
     2701 tacgctggcg gtggccgaag ctgtcgtgac aactcagcgt gactggggta accgaaccga
     2761 tcgtaaaaat gccaaaacca aatacacgct ggagcgcgtg ggggttgaga cgtttaaagc
     2821 ggaagtggag cgtcgcgcgg ggatcaaatt tgaaccgatc cgtccatatg agttcaccgg
     2881 acgaggcgat cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt
     2941 tatcgaaaat ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaaccg gcctgctgga
     3001 gatcgcgaag atccacaaag gcgatttccg cattacggcg aaccagaatc tgatcatcgc
     3061 cggtgtaccg gaaagcgaga aagcgaagat cgagaagatc gccaaagaga gcgggttaat
     3121 gaatgccgtc acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc
     3181 gctggcgatg gcggaagcag agcgtttcct gccgtctttt atcgacaaca tcgataattt
     3241 aatggcgaaa catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa
     3301 cggttgtggt cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcgc cgggtcgcta
     3361 caacctgcat cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa
     3421 catcaccgag ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga
     3481 gcgcgaagcg ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt
     3541 gctcgatccg gcgcgcgatt tgtgggatta accatcagcc cggtcttgta ggcctgataa
     3601 gaacgcgtga gcgtcgcatc aggcaaggca aacagtgagg aatctatgtc caaactcgat
     3661 ctaaacgccc tgaatgaact gccgaaggta gatcgcattc tggcgctggc ggaaactaac
     3721 gccgaactgg aaaaactgga cgctgaaggc cgcgtagcct gggcgctgga taatctgccc
     3781 ggtgaatatg tactttcttc cagctttggc attcaggcgg cggtgagcct gcatctggtg
     3841 aatcaaattc gcccggatat tccggtgatc ctcaccgata cgggttactt gttcccggaa
     3901 acctaccgct ttattgacga gttaacggac aaactcaagc tcaacctgaa agtgtaccgt
     3961 gctaccgaaa gcgcagcctg gcaggaagca cgctacggaa aactgtggga gcagggcgtt
     4021 gaaggcattg aaaagtacaa tgacatcaac aaagtcgaac cgatgaaccg ggctctgaaa
     4081 gaacttaacg cgcaaacctg gtttgctggc ctgcgccgtg aacaatccgg cagtcgcgcc
     4141 aatttaccgg tgctggcaat tcagcgtggc gtatttaaag tgctgccgat tatcgactgg
     4201 gataaccgaa ctatttatca gtacctgcaa aaacatggcc tgaaatatca cccattatgg
     4261 gatgaaggat atttatcggt cggtgatacc catacaaccc gtaaatggga acctggcatg
     4321 tcggaagaag aaacacgttt ctttggctta aaaagggaat gtggattgca cgaagggtaa
     4381 atattgccgg ataaattaaa cgcctctgtc agaaatgatg ggggcgtttt tattttgcga
     4441 gcagtattcc tgattttaaa cgtgaacgcc gctggaagaa ttatcaggta tagaatggcg
     4501 tcgggtgctt gaggctgtct ggcgttaagc attcgcgagg ttccagatgg acaaaagccc
     4561 caggcgatat ttctatcaac ctgaggccag cgttcgaacc caaacaattc gaatgttagt
     4621 ctcttctttg cagggaggca agacatgctg acaaaatatg cccttgtggc aat
//
LOCUS       DADLTD010000245         4662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLTD010000245 DADLTD010000000
VERSION     DADLTD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.249"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..755)
                     /locus_tag="JN701_003727"
     CDS             complement(<1..755)
                     /locus_tag="JN701_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY7366022.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLL"
     gene            complement(886..2250)
                     /locus_tag="JN701_003728"
     CDS             complement(886..2250)
                     /locus_tag="JN701_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine dehydratase 1"
                     /protein_id="HAY7366023.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2434..3012)
                     /locus_tag="JN701_003729"
     CDS             complement(2434..3012)
                     /locus_tag="JN701_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY7366024.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3016..4377)
                     /gene="pabB"
                     /locus_tag="JN701_003730"
     CDS             complement(3016..4377)
                     /gene="pabB"
                     /locus_tag="JN701_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY7366025.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4451..4630
                     /locus_tag="JN701_003731"
     CDS             4451..4630
                     /locus_tag="JN701_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY7366026.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1171 a         1198 c         1212 g         1081 t
ORIGIN      
        1 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
       61 cccggaccat taacgttaat ggtaaaagga aagcgttgcg aagaaacctg atagttttca
      121 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
      181 tccaccgtca ggctggcgct actgatttgc tgcagttgtg gctcaaggag cattgccgtc
      241 agtaagtcga tgttaatcat ctccatgacc ccagcattgc tactgcccgc tgcaggcgtc
      301 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
      361 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
      421 atgctggagc aatagagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
      481 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
      541 agcaagactt cgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
      601 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
      661 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgt
      721 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
      781 gacaaacgcc tgacgcaaca gtggatgtgt agtggggaat attacagcag acgagaaagc
      841 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
      901 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
      961 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     1021 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     1081 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     1141 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     1201 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     1261 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     1321 gtccgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     1381 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     1441 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     1501 gctggaaacc agcatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     1561 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     1621 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     1681 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     1741 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     1801 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     1861 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     1921 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agaagacgtt cgcgctcttc
     1981 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     2041 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     2101 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     2161 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     2221 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     2281 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     2341 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     2401 agtagtgttt tgtagatgta aagataagta tagtcagggt ttcacaccaa tttgcagcgc
     2461 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     2521 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     2581 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     2641 cggcagatcg ggcgggataa tgccgatcac tggggttacc tgatagccag ttacgctatc
     2701 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     2761 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     2821 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     2881 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttagggtttc
     2941 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     3001 acggtattcc acgtcttact tctccagttg gcgtaatatc ttagtaactt tatcaaaagt
     3061 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     3121 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     3181 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     3241 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     3301 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     3361 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     3421 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     3481 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     3541 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     3601 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     3661 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     3721 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     3781 ctggcgaaat ttttcgccgt actgctcgcg ggtcatattg gattgccagt cggaagtgag
     3841 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     3901 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
     3961 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
     4021 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
     4081 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
     4141 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttaccgaa
     4201 agtggttaaa gtgcaaatcg gctcggcgac cacaatatca aagcggctat acggatgatc
     4261 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
     4321 ttcagcggcg tcctgacgcc agggtaaagt aatcacagcg ggagataacg tcttcatcag
     4381 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
     4441 ggagttaaca atgtttgcag gtttaccttc actcacccat gaacagcagc aaaaagctgt
     4501 cgagcggatc caggaactga tggcacaagg gatgagcagc ggccaggcaa tcgcgctggt
     4561 ggcagaagag ctgcgtgcca accactccgg cgagcgaatc gtggcgcgtt ttgaggatga
     4621 agacgagtaa cacactaatc cgtatcgatt tcgttataga ag
//
LOCUS       DADLTD010000246         4628 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLTD010000246 DADLTD010000000
VERSION     DADLTD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.250"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..562)
                     /locus_tag="JN701_003732"
                     /pseudo
     CDS             complement(1..562)
                     /locus_tag="JN701_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            546..804
                     /locus_tag="JN701_003733"
                     /pseudo
     CDS             546..804
                     /locus_tag="JN701_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(913..1491)
                     /locus_tag="JN701_003734"
     CDS             complement(913..1491)
                     /locus_tag="JN701_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366027.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1985..2149)
                     /locus_tag="JN701_003735"
                     /pseudo
     CDS             complement(1985..2149)
                     /locus_tag="JN701_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2336..2765)
                     /locus_tag="JN701_003736"
                     /pseudo
     CDS             complement(2336..2765)
                     /locus_tag="JN701_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2813..3094)
                     /locus_tag="JN701_003737"
     CDS             complement(2813..3094)
                     /locus_tag="JN701_003737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366028.1"
                     /translation="MIFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWF
                     VRVDDEGYPEIARCTEREFATIPAGINADGMYCPECGTVHWPDGVIPPF"
     gene            complement(3899..4138)
                     /locus_tag="JN701_003738"
     CDS             complement(3899..4138)
                     /locus_tag="JN701_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7366029.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(4333..4628)
                     /locus_tag="JN701_003739"
                     /pseudo
     CDS             complement(4333..4628)
                     /locus_tag="JN701_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1119 a         1242 c          941 g         1326 t
ORIGIN      
        1 agaacgccat attcagtttc ccatagtgga acatcactcc cagcttaaag cgctcccggt
       61 tgcggtagcc cttggctttt attctcagca gcctgatctt gctgttcagt gcttccgcat
      121 ttccgtttga tacgccatgg cgcatggcgt tcaggatacc gtacaacctt ttccctatcg
      181 ttttagccac attcttcatc atggggacgt cactgttggc cgccagcgac agccatctct
      241 gccagtcgtt gcgtctttcc tcgctccatg gtctgctcca gatatccttt gccagttctt
      301 tcagcgccca gcacaggctc gtcagcttca tctgcgcacg cagccatatc agcttttcct
      361 gcctggattc ggtcatccat ttatcgctgt gctgccacag gaagcgagtt ccctttgcct
      421 gacgacgact ttcaacagga agatgcggat gttcattctg gcgggtttta tccaccacct
      481 cacccggttg tttcgccaca tgaaagcgat cgaaggctat tttctcaacc gcattgggta
      541 agtggatgcg cgctgctctt agtttgctga gccagtttag cattgccttc tacatgaccg
      601 atgcctgagc cggtataccg aactttattg tcctcaacca gtcatgttat aagcaagaaa
      661 tgcactcagc ggatagagcg acataatctg aacctgcgga cacatcttaa gcggcttacc
      721 cgcaaaacca tatgcttctc aaagtctgat gatatgcact ataagatcat cggctggtat
      781 ctgacaatca accaccacca ctaaatctgc gtcacgacct gcaactgagt ctggttgaac
      841 aggctgaggc cctgctgaag caacgcgaac ctctcgactc ttgttggatt ttaccaaata
      901 gtttttctaa aattaaacac atttatataa gttctttgct gaacaaaacg cagcccgcca
      961 tcctttttca ttcataaatt cagcaatggc agataaaact ttttcatcaa gcatataaaa
     1021 aataatgcaa gtagactcaa tatgcctcat acaaataagg ccgcacatat ccgtaatata
     1081 tttgtcaatg ctgctataat ttgcatggta atatgccatg ctattatctg atgattttac
     1141 atatgcagta tgacatattt ttccttcacg attaacaaaa ctgacaaaac cactttcacc
     1201 aggagtaata tataaagatg aatttaacgt gcgctcacta atattcatta attgttgaaa
     1261 ttcaatggaa tagttttcag ttaatgtatc tattatcttg gctttgtctt tctttgttat
     1321 ctcattgtct ttttcggcaa gaaaaattgc cactttatga catgcattca gctcgttaat
     1381 tttacctgat aaaactgctt ttcttaccac atcgttgaac tcatcgatag tgtgtttttt
     1441 attatatata tcatgaaaaa tatagcctaa agacttaaac gagactttca ttttatattc
     1501 tccttaaata ttaacaggat atatatatca cattttcctc tatattttca gaaaccagct
     1561 atcattttcc ccattactgg tacgtcgctt cagatatttc cgcagcaagc gataaccctg
     1621 cgcactatga ggatgccatt cattataatg tttgaacacc ttcgcgagac tactttactg
     1681 ctgttaacac gtcaggttcg aacataacac tgatttaatc atatttcatc gttttgacga
     1741 agttttctgc cattccattt tgcaccgccg tgtgtttggg ggcaaggccg accatctgtg
     1801 tttcattcgc cagatataag tgatgagatg gcattctccc tccttcacgg cagaagcatt
     1861 ttgaccgttt aggggatgcg gacgattttc atgcctgcta agatactttc gttactgatg
     1921 ctgtccgttt tccaccattt catcatcact gattggcgtt gtgtttttgg tcagcactcc
     1981 agctttacgt ttctctttta accggtaact ctctcctttg atattcagcg tgtagcagcc
     2041 gatctaggat cgctgttgcc agcacgtgat ctccgaacat ttctccccag tctgcgaccc
     2101 ctttgtttga cgtcagtatg atgctcgctt tttcatatcg acagttcatc gataggaatt
     2161 taaatcccca aaagattaaa aaagcaccga agaatgcgcg tttattcaac acaacctgat
     2221 catcaagtaa tcggtataga cacttaatat tgcttacatt gattgattca atgtatctct
     2281 gatggttttt tgctccgttc ccctcaacat ggacgcaact tcccctctgc gactctcagc
     2341 cgcaccaccg tattcgagcc agcggctcat gcatcataac ctgctctgcc agacggtaac
     2401 ccctcttcag ccccgtaaac cgcatctgac ctccgcacag ctcgcacttc agcgggtcaa
     2461 ccttcagtca ctgctgatac atccctctcc ttgtgatttg cctcgccgtt tttctcactg
     2521 tctccgttat ggcgtacacc acatcttcca gtaatcgccg cttcaccgga ctcaggaccc
     2581 cgtatcacca tccggaaccc cttatccgcc acatgccagg agaacctttc cataaactcg
     2641 tcgccactca tcacaaggtt ttcttctcgt tttgtccggt ggctgttgta acgcaggcca
     2701 atttcatcct gaccggcata atgttccagg cggctcatct gcaccggctg cttctccagc
     2761 cagttaagcc atcggtttcc ctgctgaaaa atatcggcaa aacggggaag catcagaaag
     2821 gcggaataac gccatccggc cagtgaaccg taccgcattc agggcaatac atgccgtcgg
     2881 cgttgatacc ggcaggaatt gtcgcaaatt cccgctccgt gcagcgggca atttccggat
     2941 acccttcgtc atcaacacgt acaaaccaga agaccagttt tttgtttccg gcatccacaa
     3001 agaacggaat attcaggtct gcgcaacact caacggcatc gtcaaaatta tcaaagcgca
     3061 gaacctcagc ttcttcttcg tcaaaaaaaa tcatcttcgt gaagttttac gacatagtgg
     3121 aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg gtttagctga acgggccata
     3181 taactacctg aaagacaatg atattgtctg tttttataac ggtaatcaca ggccatgaca
     3241 agccgcagcc gtcaggccgc ctactcgggg cggtttagaa cgcttattta gtaccagatc
     3301 ttacttataa taaaggggct tggcagtttg gataaactat tgtccttatt ccactaatac
     3361 aaactttttg taaagtatat taaatatact tcatttcata ctgaaatccc cggaagtatt
     3421 caatcaaaca attacaccat aaaaggaaga gggggcccca attaagttgt caagcatgtt
     3481 tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca ttggtactct ggcgctcatg
     3541 gggcttccac ttgcgcttct cccttcacat cggggtgaca ggtccacgtt ccccacaaga
     3601 gcctgaatcg acttcacgcc gcttttatgc cggatgccgc gcaggcagta aacatatccc
     3661 acaggaacga ctccaccatt gttttgacat catctatatg ctttcgacac atcatcagcg
     3721 gttcactttc gttcgtctct tcgaaccata cctgatcggc tcacgtccga ccttttccca
     3781 tctcgctcac caccacatct cttaaatgca gcagcagtgg atggtttggt gcctgcgcct
     3841 gcacgccgac accgaggggc cttccctcat ctcttgtgcc gcatggctgc acagggactc
     3901 acgtccccag gacgcgcctt cgtggcacac agtcatcggc atagcaacag taagcaacgg
     3961 caggtttcca ttgccagttt tctttgacag cagtacttcg gcctcgctgg atactgtgat
     4021 tccagtacca gcgatccttt ctggcttttc cactcaggta gcgtttattc agatactggt
     4081 cgaactcatt cagtatgatg ttcgacagca acggcgagat cacaccgccc tgtggcatac
     4141 tttcactgac cgcccggaag agaccaacat caacacggcg ttttccacag cagtctcata
     4201 aaacgtgcat cactgatcct gcggcagacg gctttcatca gcagccggtg atgtactgtg
     4261 tcgaagtagc tggacaggtc accttcaata acccggcgcc ccgtgtttcc ccgcagtcgg
     4321 tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg ttcgggccac tggcgtaaaa
     4381 atcctgcgtc agcagatttt ctgatacagg caggccgtgt gcacggtaac tgatcgggct
     4441 gaacttctgg gaggcttttg cgctcagttc ctgacggcgc agactggctg ccacggtttt
     4501 tacgttaaag tggtaaccag agcacacagt tcatccgtca ggcgtggggc accgtaacgc
     4561 tggactgggt aaaagccttg cggacaacgc tgtcgcagtg ctgacggaac tgctgacgtg
     4621 tgtttatc
//
LOCUS       DADLTD010000247         4586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLTD010000247 DADLTD010000000
VERSION     DADLTD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.251"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            250..615
                     /gene="queD"
                     /locus_tag="JN701_003740"
     CDS             250..615
                     /gene="queD"
                     /locus_tag="JN701_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY7366030.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            693..1961
                     /locus_tag="JN701_003741"
     CDS             693..1961
                     /locus_tag="JN701_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7366031.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1952..2212
                     /locus_tag="JN701_003742"
     CDS             1952..2212
                     /locus_tag="JN701_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7366032.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            2229..2804
                     /locus_tag="JN701_003743"
     CDS             2229..2804
                     /locus_tag="JN701_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY7366033.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2952..3812)
                     /locus_tag="JN701_003744"
                     /pseudo
     CDS             complement(2952..3812)
                     /locus_tag="JN701_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(3809..>4586)
                     /locus_tag="JN701_003745"
     CDS             complement(3809..>4586)
                     /locus_tag="JN701_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY7366034.1"
                     /translation="NILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADEQ
                     AAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPEF
                     VARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFIT
                     LEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPAM
                     QCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
BASE COUNT         1052 a         1235 c         1166 g         1133 t
ORIGIN      
        1 agataaggtt aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc
       61 aaatcgacta acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat
      121 gaaaaacaaa tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc
      181 gttttagata atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt
      241 agagaaatta tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc
      301 ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg
      361 cgactggaaa ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa
      421 ctaaaagcgg cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt
      481 ccaggtctgg aaaacccaac tagcgaagtt ttagcaaaat ggatttggga tcaggttaaa
      541 cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc tggttgtatc
      601 tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta
      661 gggtattgtg ttcaggatat ctggggcatg acatggaaga cgactgcgac attattatta
      721 ttggtgccgg tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg
      781 ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc ggacggttat
      841 atacccatgc actcgcggaa ctcctccctc agtttcatct gaccgcgcct cttgaacgac
      901 gcatcactca cgaaagcctt tccctgttaa cgcccgatgg cgtaacgacg ttttccagct
      961 tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg
     1021 ccgaagccga aaaagaaggt gtcgaatgta tccccggagc gacggtggat gcactgtatg
     1081 aagaaaacgg cagagtctgt ggcgttattt gtggtgacga tattctccgc gcccgttatg
     1141 tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc
     1201 ctgctggcga agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta
     1261 ttgaagaacg ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggaa
     1321 tctgtgatga cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag
     1381 ggattgtttg cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctga
     1441 ctcgctttaa agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt
     1501 atggtgcgca tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta
     1561 acggctggca gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc
     1621 gcggcatggg tatggcgctg actggcgcgc aggcggcaca aacgctgata agcgcctgcc
     1681 agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca caacgtagag cgcagcctgc
     1741 tatgggatgt tctacaacgt tatcagcatg ttccggcgct tttgcaacgc cctggctggt
     1801 atcgggcgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata
     1861 aacctgttcc accgctgcgc cagttattct ggcgtcattt acgtcgtcat ggcctgtggc
     1921 atctggcggg cgatgttatc aggagtctgc gatgtctgta gcccataatc tctggcgcgt
     1981 tgctgatgcg ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaatggtt
     2041 gattaacgcc tgtccggcag gtcttttttc gctcacaccg gaaggtgact tacgcgttga
     2101 ttatcacggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca
     2161 acagtggcgc tatccgcctt ccggattcgg catcacctac cgctttggat aaataataag
     2221 gataatttat gcccctctta cacctgctcc gccagaatcc ggtgattgct gccgttaaag
     2281 ataatgccag cctgcaactg gcaatcgact ctgaatgcca gtttatttcc gtgttgtacg
     2341 gcaatatctg caccatcagt aatatcgtta aaaagattaa gaacgccggg aaatatgctt
     2401 ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtcgtgatt cagtttttga
     2461 aactggtgac cgaggcggac ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa
     2521 gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcaatt tcgtttcaca
     2581 acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgta
     2641 tgcccaaagt gctgggctgg gtgacagaga aaatccgcca accgctgatt gccggtgggc
     2701 tggtgtgcga tgaagaagat gcgtgtaatg cgattaacgc gggtgtcgtg gcgctttcca
     2761 ccacgaatac cggggtctgg acgttagcga aaaaattact ttgacgggat aatcgtaacc
     2821 aattgaattt ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg
     2881 acgcttgacg cgtcttatca ggcctacaag tccccaggcc agaaccgtag gcaggatcat
     2941 ccggcaacac attattggca atctgcgtga atgtttgtca ccagtgcctc cagcaccact
     3001 ttccagtcat caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga
     3061 ttaatcgcca ccacaaactt gctattgcgt acgcccgcca tttacgctgc tgcgccggaa
     3121 gcgccaacaa caatacacac ctcaggtgcc agcaaatgtc cggaaatacc aatcactttt
     3181 tcagcatcaa cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc
     3241 ttttctgcca gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccagc
     3301 acccgtctgg cctcagctaa tgggtcgcga gtaacatttt tcagatcctc tgtgctaacc
     3361 agccagtcgg gaagcgcacc cgggacaatg ttcagttgtt gtattccaga gggcaacgtg
     3421 gcgtttttcg ctgctcccgc ctgacgcgcc agagaaaggc atagcgggcg tttttctgtt
     3481 tgcagcgtcg ccgttagcgc atttccccag tgggatttgc gcacgcttac ggttggaata
     3541 tctaacgaag tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgggtcgac
     3601 agttcatcgc caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac
     3661 tgttccacca acgcatccag cacctgttcc gccactacag gctgaggttc aatctgccag
     3721 tgcgccaggg tacaaccact gaaatcctgc gccgccagcc agcttgctat tgcggcattc
     3781 tcctgattta ttgtcacaat tgcgatattc atggctgcat cctttggcgt aaatagtcct
     3841 gccatagctt ttgagctttt tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc
     3901 gttgctctgc tcttgccagt tgcagacact gcatcgctgg catttcggca gcaacagttt
     3961 tacggataat ttctgctttc cccgcagcca ttcgctggcg cattcccggt actggcaagg
     4021 ccacttcacc gcactgacgc acggcaatca ccgcaggcaa gcgaacccgg caacaacgca
     4081 acccatgttc agtacgctgt tcgagggtga taaacagcgc gtcgagagtg aaacgctcca
     4141 cctgggtgaa gcaggtccag cccagcattt ccgccagcaa aaatggcgtt tgcccattct
     4201 gcccttcgct gctttggctg ccggtaatta tcagatccag cggattctga tgctgccatt
     4261 cggcaatatg gcgagcgaca aattccggcg caaagcgcag atctgccgcc gtctccagca
     4321 atacagcttc ctcaaaccca agagccatga gatagcgcag ccagtgcaat gcccgttcat
     4381 cccccatgct caacgcggtt aaagacatcg gcgtaccgtt ttttctctgc gccaacagca
     4441 gcgcggcagc ggcctgttca tcagcaccga gtaaacttcg cagtagcgaa atatccggtc
     4501 cgcttttacc ctgagccgcc gcctgccact ctttttccgc cagcattccg gcatccggtt
     4561 cggctttaaa cgctaacaga atgttc
//
LOCUS       DADLTD010000248         4560 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLTD010000248 DADLTD010000000
VERSION     DADLTD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.252"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            92..850
                     /locus_tag="JN701_003746"
     CDS             92..850
                     /locus_tag="JN701_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAY7366035.1"
                     /translation="MSFMSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQR
                     MQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRR
                     WQALVENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNK
                     ILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIES
                     PRTGETIRVSRLAEPFWQDHFLGARWILTEETIL"
     gene            1026..1523
                     /gene="rayT"
                     /locus_tag="JN701_003747"
     CDS             1026..1523
                     /gene="rayT"
                     /locus_tag="JN701_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY7366036.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(1747..3486)
                     /gene="flhA"
                     /locus_tag="JN701_003748"
     CDS             complement(1747..3486)
                     /gene="flhA"
                     /locus_tag="JN701_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY7366037.1"
                     /translation="MLSRSDLLTLLTINFIVVTKGAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIILTRVSDNGDIAHDVRN
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMAQPSSETYGEIDGVL
                     GNDPAYGMPVTWIQPAQKAKVLNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            3431..4216
                     /gene="lafU"
                     /locus_tag="JN701_003749"
     CDS             3431..4216
                     /gene="lafU"
                     /locus_tag="JN701_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY7366038.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHIDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
BASE COUNT         1087 a         1201 c         1193 g         1079 t
ORIGIN      
        1 tgaaacctca atagtaatat gcaaataagc aattaataat cccgtttact cctccggtct
       61 tttaaaattc tcaccttcat tcagccggtt tatgtccttc atgtcttcgt tcttgctcgg
      121 cagatttctg catccaggcg tgttttcgtt atgcgttttg ctccctttac tcgccagcgc
      181 taccacatca catatctctt tcagctacgc cgcccgccag cggatgcaaa accgtgcgcg
      241 tttattaaaa cagtaccaaa cccatctgaa aaaacaggcc agctacattg tggaaggcaa
      301 tgccgaaagc agaagggcgc tacgccagca caaccgggag cagataaaac agcatccaga
      361 atggtttcct gcgccgctca aggcaagtga cagacgctgg caggcgttgg tagaaaacaa
      421 tcatttcctc agcagcaacc acctgcataa tatcaccgaa gtggcgattc accgtctgga
      481 gcaacagctt ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag gcttcgactg
      541 tagcgggttg gttttttatg cctacaacaa gatccttgag gctaagctcc cgcgcacggc
      601 taatgagatg tatcactacc atcgggcaac cattgtggca aataacgacc tgcgccgggg
      661 agatttgctg tttttccata tccacagccg tgaaattgcc gaccatatgg gcgtgtatct
      721 gggagacggt cagtttattg agtcgccgcg cacgggggaa accattcggg taagtcggtt
      781 agctgaacct ttctggcagg atcatttttt gggcgcgcgg tggatattga cggaagagac
      841 gattttgtag gacggataag gcgtttacgc cgcatccggc ggtggtgcgc agatgcctga
      901 tgcgacgctg acgcgtctta tcatgcctac aaatctgtgc gtttttgcga gccgctttcc
      961 tgttattcca tcacctcatt gccgaccttt ccttttcttc ttaccgttga gaaaaaggag
     1021 tcgccatgtc tgaatatcgt cgttattaca tcaagggggg aacctggttt ttcacggtga
     1081 atttacgcaa tcgtcgaagc caactcttga ccacccagta ccagatgctc cgtaacgcca
     1141 ttattaaagt taagcgagac aggccttttg aaatcaacgc ctgggtcgtt ttgccagagc
     1201 atatgcattg catatggaca ttacctgaag gcgatgatga tttttcctct cgctggcggg
     1261 aaattaaaaa gcaatttacc catgcttgtg gattgaaaaa tatctggcaa ccacgttttt
     1321 gggagcacgc catccgcaat accaaagatt ataggcatca tgttgattat atttatataa
     1381 atccagtaaa gcatggttgg gtaaagcaag tgagtgattg gccattctca acgttccatc
     1441 gcgatgtcgc gcgagggtta tatcccatcg attgggcggg ggacgtaacg gatataaatg
     1501 ctggcgagcg tatcatttta taattatgcg cagatacctg atgcgacgct ggcgcgtctt
     1561 atcatgccta caaacctgtg ccggatcggt aggccggata aggcgtttat gccgcatccg
     1621 gcggttatgc gcagatgcct gatgcgacgc tggcgcgtct tatcatgcct acaaacctgt
     1681 gctggatcgg taggccggat aaggcgttca cgccgcatcc ggcagttgtg caccaatgct
     1741 ggatacttac atcaacgccc ccataatctt caactccagc tcgtccggca cttcgttata
     1801 cgacagcaca tgcagccccg gcgcaaacaa ccttgcataa cgcgccagca aagggcgcag
     1861 ctgcggtggc accagcagca ccgggtcttt ccccgccgct ttcatctgct ctttcacctg
     1921 tggcatcgta ctctggaact ggttgagcat attcgggtcc actggcacgc tgtcgagcat
     1981 cactttaccg ccctgttgcg cctgattcac taggttagtc agcagatttt ccagctcatt
     2041 attcagggta tacaccgtca gctcctgctt gcgaacgaac ggatgggtaa tgctgcgccg
     2101 cagcgccagg cgcacatcgg ctgccagcag aatatgatct ttggtcaccg cgctactggc
     2161 gaccagcacg gtggcgatgg tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc
     2221 ccggtacact ttcagcaact ggctgtaatt gagcaccgcg cttaaatcct ccgccaggcg
     2281 cggtgccatc gacgacaaac ggttatgcaa ctgcgtaata tcgtcatagt taaataaatc
     2341 aggaatatag ctgcgcacaa tcttatttac atgggtagca atcacgctgg cgctgtcgat
     2401 cacctgatac cccatattca gcaccttcgc tttctgcgca ggctggatcc aggtcaccgg
     2461 catcccgtac gccgggtcgt tccccagcac gccgtctatc tcgccgtagg tttcgctgga
     2521 gggctgggcc atcagtttat ccgccggaat atccgcttca tcagccttaa tgccgttgat
     2581 gaaaatagcg tactggctgg gcttaaggcg gaagttttcc cgaatgcgga tctccggcag
     2641 cagcacgccg ttgccgtcgg atatcacctg tcgcacgccg cgaatacgct gggtgagcgg
     2701 gttgccctgg gctttatcga ccagcgccac cagcttgtag ccgaggctta aactgatagg
     2761 ctcgataagc ggaatggttt cccaactgac ctgttgctcg ctggtttcgg tgatggtgcg
     2821 ggtcagcgtt tcgagacttt tctcttccgc ttccgccgcc tgcgggcgtt tgctcatccg
     2881 ccagccggta aagccaagca gggcgctgaa cagcaaaaac ggcagatgtg gcattcccgg
     2941 cactaccgcc agtacgaaca taattccggt agcggtgtag agcaccgatg ggcttgccag
     3001 caactggtta cgcacgtcat gggcgatatc gccgttgtcg ctgacgcggg tgaggataat
     3061 cgccgccgcg gtggagagta gcagggaagg gatttgcgcc accagcccgt cgccgatggt
     3121 catcagcaca tactgctgga aggcggcgtc ggcgctcagg ttgtatttga agatcccgat
     3181 acagacgccg ccgatcaggt tgatcgccag aatcatcatc ccggcgatag catccccgcg
     3241 cacaaatttc gatgccccgt ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt
     3301 acgtcgggtt tgtgcctgcg cctgattgat caacccggca ttaagatcgg cgtcaatcgc
     3361 catctgctta ccgggcatcg catccagggt aaagcgggct gaaacctcgg aaatacgctc
     3421 ggcccctttg gtgacgacga taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga
     3481 aagcattatc gccgcgctgc acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt
     3541 aaataaaatc agcccgtctc atccgccaaa acctgccacc gtggcggtgc cggaagaaac
     3601 ggagaaaaaa gcgcgcgacg tcaatgagaa aacggcgctg ctgaagaaga aatccgccac
     3661 cgagcttggc gagctggcaa ccagtatcac caccattgcc cgtgatgcgc atatggaagc
     3721 gaatctggag atggagattg ttccccaggg attacgcgtg ctgattaaag acgaccagaa
     3781 tcgcaatatg ttcgaacgcg gcagcgcgca gattatgccg ttctttaaaa cgctgctggt
     3841 ggagctcgcg ccagttttcg actcgctcga taacaaaatt attattaccg ggcatatcga
     3901 tgcgatggct tacaaaaaca atatctacaa caactggaac ctttcgggtg accgcgcgct
     3961 ttcggctcgt cgggtgctgg aagaggccgg aatgccggaa gataaagtga tgcaggtaag
     4021 cgcaatggcg gaccagatgc tgctggattc caaaaatccg caaagcgcgg gcaaccggcg
     4081 cattgagatt atggtgctga ccaaaagtgc gtccgatacg ctgtatcaat actttggtca
     4141 gcatggggat aaagtggtgc agccgctggt gcaaaagctg gataagcagc aggtgctttc
     4201 gcagcgaacg cgttaaatgc tgaatcttta cgcatttctc aaactctgaa atcactgtat
     4261 actttaccag tgttgagagg tgagcaatgc gtaaaatcat gattttcact tcctaaaaca
     4321 ccccttataa tctggtcata gagtcatcat agcactggat gaatatacag atgaggaagg
     4381 tttacagtta cattcgcttt tcgtcaacga aacaagcttt tggtgatagt catcgaagac
     4441 agagtaaggc catacaggat tggttagcca gccatccaga ccacatccta gacgaatctc
     4501 ttagttttga agatttagga cgttcagcct ttcacggtga ccatctaaaa gaaggtggag
//
LOCUS       DADLTD010000249         4440 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.253, whole genome shotgun sequence.
ACCESSION   DADLTD010000249 DADLTD010000000
VERSION     DADLTD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.253"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            127..456
                     /locus_tag="JN701_003750"
     CDS             127..456
                     /locus_tag="JN701_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7366039.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            704..949
                     /gene="nrdH"
                     /locus_tag="JN701_003751"
     CDS             704..949
                     /gene="nrdH"
                     /locus_tag="JN701_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY7366040.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            946..1356
                     /gene="nrdI"
                     /locus_tag="JN701_003752"
     CDS             946..1356
                     /gene="nrdI"
                     /locus_tag="JN701_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY7366041.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1329..3473
                     /gene="nrdE"
                     /locus_tag="JN701_003753"
     CDS             1329..3473
                     /gene="nrdE"
                     /locus_tag="JN701_003753"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY7366042.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3483..4397
                     /gene="nrdF"
                     /locus_tag="JN701_003754"
     CDS             3483..4397
                     /gene="nrdF"
                     /locus_tag="JN701_003754"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY7366043.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSRIGN"
BASE COUNT         1095 a         1136 c         1134 g         1075 t
ORIGIN      
        1 cgggcctacc ttacctgatt gcgcattcac aacgctttca gaaaagtcca taaagccgcg
       61 atgaacctca gttaagaaaa ttatggtcta cactgaaaat tacatcgaat tctaatgggg
      121 gatcatatgt ttaaccgacc gaaccgcaac gacgttgatg atggcgtgca ggatatccag
      181 aatgatgtca atcaattagc tgacagcctc gaatctgtat tgaagtcctg gggcagcgac
      241 gccaaagggg aagctgaagc cgcacgcagt aaagctcagg cgttgctgaa agaaaccagg
      301 gcgcgaatgc atggtcgtac tcgcgtccag caagccgcgc gcgatgccgt tggctgcgcg
      361 gattcttttg ttcgtgaaag accctggtgt agcgtgggta cagcagctgc ggtaggtatt
      421 tttatcggcg cactgttaag catgcgcaaa tcgtagtgca aaaatgataa taaatacgcg
      481 tctttgaccc cgaagcctgt cttcggggtt tctttttgcc tggtgaatca caaaaatccc
      541 cctcccccgt cacgctcata tccagggtaa tttcgatcac tatttgctat atattgtgta
      601 gttgaatctt ttttcaacta catctagtgt ctctgtatca acagagagac aacccgacgc
      661 gtatcatcgc gccgtatctt cattttaaac ggaaatacga atcatgcgca taactattta
      721 cactcgtaac gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accgggggtt
      781 tgattttgaa atgattaatg tcgatcgcgt tcctgaagca gcggaagcgt tgcgtgctca
      841 gggctttcgt cagttgccgg tagtgattgc tggcgatctt agctggtctg gtttccgtcc
      901 ggacatgatt aaccgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc
      961 tacttctcca gcagctccga aaacacgcag cgttttatcg aacgtttagg tctgcccgcg
     1021 gtgcgcatcc cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc
     1081 gtgccctctt acggcggcgg cggtacggct ggcgcggtgc cacgacaggt aattcgcttt
     1141 ttaaacgacg agcacaaccg ggcgttgctt cgcggcgtta ttgcttctgg taatcgcaac
     1201 tttggtgagg cgtatggccg cgccggagat gtgattgccc ggaaatgcgg cgtgccgtgg
     1261 ctgtaccgtt ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta
     1321 accgaatttt ggcaacgaca accgcagaat gcctgacgca ggaaacgatg gattaccacg
     1381 cgctgaatgc gatgcttaac ctctacgata gcgcaggtcg cattcagttc gataaagacc
     1441 gccaggccgt tgacgccttt atggcgacgc atgtgcgtcc gaacagtgtg accttcagta
     1501 gccagcagca gcgcctgaac tggctggtca acgaaggtta ctatgatgaa agcgttctta
     1561 atcgctactc tcgcgacttt gtcattacgc tgtttgccta cgcacacacc agcggttttc
     1621 gtttccagac attcctcggg gcatggaagt tttacaccag ctatacgttg aagacattcg
     1681 acggtaaacg ttatctggaa gattttgccg atcgagtcac gatggtggcg ctgacgctgg
     1741 cacaaggcga tgcgacgctg gcgttgcaac tgaccgatga aatgctgtca ggacgctttc
     1801 agccagccac tccgacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct
     1861 gttttctgct gcgtattgaa gacaatatgg agtcgattgg ccgggcggta aattccgcac
     1921 tgcaactgtc gaaacgcggc ggcggcgtgg catttttgct gtcgaatctg cgagaagcgg
     1981 gcgcgccaat taaacgtatt gaaaatcaat cttctggcgt aattccggtg atgaaaatgc
     2041 tggaagacgc attttcctat gccaaccaac tcggcgcgcg tcagggggca ggtgcagtct
     2101 atttacatgc tcatcatccc gatattctgc gttttctcga tacaaaacgg gaaaatgccg
     2161 acgaaaaaat ccgcattaaa acactgtcgc tgggcgtggt gatcccggat atcactttcc
     2221 atctggcaaa agagaatgcg cagatggcgc tgttttcgcc ctatgacgtt gagcgagttt
     2281 atggcaagcc gtttgccgat atcgccatca gcgaacacta tgacgaactg gttgccgatg
     2341 aacgcatccg caaaaaatac ctcaacgccc gtgatttctt ccagcggctg gcagaaatcc
     2401 agtttgagtc cggctatccc tacatcatgt atgaagacac ggtaaaccgt gctaaccctg
     2461 tcgccgggcg cataaatatg agtaatctct gctcagagat tttgcaggtc aactgtgcct
     2521 cagagtatga cgagaatctc gactatgccc gcacaggcca tgatatctcc tgcaatttag
     2581 gttcgttgaa tattgcgcac accatggatt cccccgattt tgcccgcacg gtagagactg
     2641 ccgtgcgcgg tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg
     2701 aagccggaaa tgccgcctca cacgccatcg gactggggca gatgaattta cacggctatc
     2761 tggcgcgaga aggcatcgct tatggttcgc cggaagcact ggatttcacc aatctctatt
     2821 tctatgccat cacctggcac gcactgcgta cctcgatgtt gctggcgcgc gaacgcggtg
     2881 aaaccttcgc cgggttcaaa cagtcacgtt atgccagtgg tgaatatttt agccaatatc
     2941 tgcaaggtaa ctggcagccg aaaacggcga aagttggcga actgtttgcc cgcagcggta
     3001 ttacgttacc tacccgtgag atgtgggcgc aactgcgcga cgacgtgatg cgctacggca
     3061 tttataacca gaatcttcag gcggtgccgc caaccggttc tatctcttat atcaaccatg
     3121 ctacgtcgag tattcatccg attgtggcga aagtagagat acgcaaagag ggcaaaacag
     3181 gacgcgttta ctaccctgcc ccgtttatga ctaacgagaa tctggcgctg tatcaggatg
     3241 cttacgaaat cggcgcagaa aagatcatcg acacctacgc cgaagcgact cgccatgtcg
     3301 atcaggggct gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca
     3361 aagcgcagat ttacgcctgg cgcaagggta tcaaaacgct ctattacatt cgcctgcgtc
     3421 agatggcgct ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat
     3481 ctatgaaact ctcacgtatc agcgccatca actggaacaa gatatctgac gataaagatc
     3541 tggaggtgtg gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccgctgtcga
     3601 acgatattcc tgcctggcag acattaactg tcgtagaaca acaactgacg atgcgcgttt
     3661 ttactggcct gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc
     3721 cctatgcact cacgcctcat gaagaagcgg tattatcgaa tatcagcttt atggaagcgg
     3781 ttcatgcccg ctcttacagt tcgattttct cgacgctgtg tcagaccaaa gatgtcgatg
     3841 ccgcctacgc ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag attattcagc
     3901 aacattatcg cggtgatgat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt
     3961 ttttgttcta ttccggtttc tggctgccga tgtatttttc cagccgcgga aagctgacca
     4021 ataccgcaga tctgatccgt ctgattatcc gcgatgaagc agtccacggt tactacatag
     4081 gctataaata tcagaaaaac atggaaaaga tatctctggg acaacgtgaa gagttgaaga
     4141 gtttcgcctt cgatttgttg ctggaactct acgacaacga gttgcaatac agcgatgagc
     4201 tgtacgccga aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac aacgccaata
     4261 aggctttgat gaatctgggc tacgaaccgt tatttcccgc agaaatggcg gaagtgaatc
     4321 cggcaatcct cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc ttttccggtt
     4381 cacgtatagg aaattgaaaa actaagcgat acccctgtcc gcgccttata ccacgtgagc
//
LOCUS       DADLTD010000250         4436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.254, whole genome shotgun sequence.
ACCESSION   DADLTD010000250 DADLTD010000000
VERSION     DADLTD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.254"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1088
                     /gene="nadB"
                     /locus_tag="JN701_003755"
     CDS             <1..1088
                     /gene="nadB"
                     /locus_tag="JN701_003755"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY7366044.1"
                     /translation="VGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSGD
                     GIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPDF
                     DERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGIDL
                     TQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSFLECLV
                     YGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIVR
                     TTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRGL
                     HFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="JN701_003756"
     CDS             complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="JN701_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY7366045.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1942..3276
                     /gene="srmB"
                     /locus_tag="JN701_003757"
     CDS             1942..3276
                     /gene="srmB"
                     /locus_tag="JN701_003757"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY7366046.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3485..4366)
                     /locus_tag="JN701_003758"
     CDS             complement(3485..4366)
                     /locus_tag="JN701_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7366047.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1125 a         1209 c         1124 g          978 t
ORIGIN      
        1 ttgtaggcgc gtgggtatgg aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag
       61 cagtggtact ggcaaccggc ggtgcgtcga aggtgtatca gtacaccacc aatccggata
      121 tttcttctgg cgatggcatt gctatggcgt ggcgcgcggg ctgtcgggtt gccaatctcg
      181 aatttaatca gttccaccct accgcactgt atcacccaca ggcacgcaat ttcctgttaa
      241 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
      301 ccgattttga tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc
      361 atgaaatgaa acgcctcggc gcagattgta tgttcctcga catcagccat aagcccgccg
      421 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggattgatc
      481 tcacacaaga accggtaccg attgtgcctg ctgcacacta tacctgcggt ggtgtaatgg
      541 ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata
      601 ccggcttaca cggcgctaac cgcatggcct cgaattcatt tctggagtgt ctggtctatg
      661 gctggtcggc ggcagaagat atcaccagac gtatgcctga tgcccacggc gtcagtacgt
      721 taccaccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
      781 actggcacga gctacggctg tttatgtggg attacgtcgg cattgtgcgc acaacaaaac
      841 gtcttgagcg cgccctgcgg cgtatcaata tgttgcaaca ggaaatagac gaatattacg
      901 cccacttccg cgtgtcgaat aatttgctgg agctgcgtaa tctggtgcag gttgcggaat
      961 tgattgttcg ctgtgccatg atgcgcaagg agagtcgtgg cctgcatttc acactggatt
     1021 atccggaact gctcacccat tccggtccgt cgatcctttc ccccggcaat cattacataa
     1081 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
     1141 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
     1201 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
     1261 tgcttaatgc cagctccgta aaaccattac caatctgctc tggcagcact acgcagaaaa
     1321 atccctcttc ggtaatgcac tccgccgcac aggtcagcag cgatgggtga tcaagcgtag
     1381 tggtatagcg agcctgttcc cgttgaggga tcgcgcactc tactccctgc tgatagtaag
     1441 gtgggttact gatgattaaa tcgaagcgta ctgtctgctg tgtgagccac tgctgaatat
     1501 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgcct
     1561 gcgcggcagc ttcactttcc agctcaacgg catcaattat cacgctgtca ctggttcgct
     1621 gcgccagcat cagtgccagc aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc
     1681 cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
     1741 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag
     1801 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
     1861 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
     1921 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
     1981 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
     2041 gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt
     2101 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa
     2161 tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc
     2221 gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat taccggcggc
     2281 gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg
     2341 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
     2401 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat
     2461 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
     2521 gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat
     2581 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa
     2641 cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
     2701 tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc
     2761 aacaactgct atctcgaagg tgagatggta cagggcaagc gtaatgaagc gattaaacgc
     2821 ctgaccgaag gccgcgtaaa cgtactggta gcaaccgatg ttgccgcgcg cggtatcgac
     2881 attccagacg tcagccacgt ctttaacttc gatatgccgc gcagtggtga tacttatttg
     2941 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcgatttc gctggtggaa
     3001 gcccatgacc atctgctgct tggcaaagtt ggccgttata ttgaagagcc gataaaagct
     3061 cgcgtcattg aggaactacg cccgaaaacg cgtacgccaa gcgagaagca gaccggcaag
     3121 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaag
     3181 ccgcgggtga aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccgagc
     3241 ggaacgggcg tgccaccgca aacgacagaa gagtaatctt aattccgggt ttaagcccgg
     3301 aattaaaagt gctggcaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacatg
     3361 gctcatgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt tcacgccgca
     3421 tccggcatga acaaaacgca cttcgtcgac aagttgggtg ccgggtttaa gcccggcatt
     3481 atttttatcc cggcaaaaaa cactcttcaa tacactgaat aaaagtgtgc atcgccgggc
     3541 taaccgcttt tccagcatgg tgtgcgcaca tagccgtaat ggtctgcgac tgttcgccaa
     3601 aaggcaattc aattaattct ccgctctcaa gctctttttc caccgcaaag cggggcaagt
     3661 aactgacacc gatattcgcc gcgacacaac gtttgatact ttcaatgctt aacagctcaa
     3721 tggtgttttc caccgtgatc cgccgctgac gcagcgtgct ctcaaatatc tgccggaaga
     3781 cacattgcgg ttcgttgata ataaagctac aggcgttatg tcttcccggc tcagtaaaat
     3841 cgacatctgc aatttgcggt gaagccacca gcaccagcga ttgttcaccc aactctcgtc
     3901 ggttcagggc atcatcattc cctacgcgat aaaagacgcc gacatcggcc tcatcattca
     3961 gtagcgcatc acggatcacg taacagttca gcgactgcaa cgataaacgc acttttggtg
     4021 cgcgttgccg aaaacgctgc aacacctgcg gcattcggta agagagcaac gtttcgcccg
     4081 aaacaacgcg tagttccccg tccggatctg accccttttt ggcagcctca cgaagcgtat
     4141 ccatcactcg ggtaagttca taaatgtgcg gcagcaactt ttttccttcg cgggtaaggc
     4201 acatccgtcg gccaattttc tcaaataact ggactgaaaa ctcctgctca agctgctgaa
     4261 tatgaaaagt caccgtcgat tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac
     4321 cctcttccac cacggtttta agcgtaataa aacggcgcag atccataacc ccgaacctat
     4381 cgaaaatatc gaatctagaa tataaaaaca ttcatttttt taaatgttct gtgtcg
//
LOCUS       DADLTD010000251         4431 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLTD010000251 DADLTD010000000
VERSION     DADLTD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.255"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1220
                     /locus_tag="JN701_003759"
     CDS             <1..1220
                     /locus_tag="JN701_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7366048.1"
                     /translation="VHNAARIRYPMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDM
                     FYEELERVQKTHGPSALLTASGWQSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAA
                     QVILPRVVGSMEVYEQQTSWPLVLQNSKTIMLWGSDLLKNQQANWWCPDHDVYEYYEQ
                     LKAKVAAGEIEVISIDPVVTSTHEYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLY
                     DKNFLANYCVGFEQFLPYLLGEKDGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQI
                     IAGWCVQRMQHGEQWAWMIVVLAAMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGF
                     SGSTSIPPVHDNSDYKGYSSTIPIARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGT
                     NPFHRHQQINRII"
     gene            1233..2270
                     /locus_tag="JN701_003760"
     CDS             1233..2270
                     /locus_tag="JN701_003760"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7366049.1"
                     /translation="METVIAIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGI
                     IAMKQVVPPQFEARNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRG
                     VHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNY
                     DDCQGHPMWFEKIERSHGGPGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKE
                     PVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGG
                     EPGALCKYGNPNVLTIDIGTSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEIVAQ
                     CEYVPASQVKS"
     gene            2267..2866
                     /gene="torD"
                     /locus_tag="JN701_003761"
     CDS             2267..2866
                     /gene="torD"
                     /locus_tag="JN701_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY7366050.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(2959..3264)
                     /gene="cbpM"
                     /locus_tag="JN701_003762"
     CDS             complement(2959..3264)
                     /gene="cbpM"
                     /locus_tag="JN701_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY7366051.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(3264..4184)
                     /gene="cbpA"
                     /locus_tag="JN701_003763"
     CDS             complement(3264..4184)
                     /gene="cbpA"
                     /locus_tag="JN701_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY7366052.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         1041 a         1129 c         1227 g         1034 t
ORIGIN      
        1 acgtacacaa cgcggcgcgt attcgttatc cgatggtacg cgtggactgg ctgcgtaagc
       61 gccatctcag cgatacctcc cagcgcggtg ataaccgttt tgtgcgcgtg agctgggatg
      121 aagccctcga catgttctat gaagaactgg aacgcgtgca gaaaactcac gggccgagtg
      181 ccttgctgac cgccagtggt tggcaatcga cggggatgtt ccataacgct tcggggatgc
      241 tggcgaaagc tattgccttg catggtaata gcgttggtac gggcggagat tactctaccg
      301 gtgctgcgca ggtgatcctg ccgcgcgtag tcggttcgat ggaagtgtat gaacagcaaa
      361 cctcctggcc gctggtattg cagaacagca aaaccattat gctgtggggc tccgatttgc
      421 tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga tgtttatgaa tattacgagc
      481 aactaaaagc gaaagtcgcc gccggtgaaa ttgaggtcat cagcatcgat ccggttgtca
      541 catccaccca tgagtatctg gggcgcgagc atgtgaagca cattgcggtt aacccgcaaa
      601 ctgacgtgcc gctgcaactg gcgctggcgt atacgctgta cagtgaaaac ctgtacgaca
      661 aaaacttcct cgctaactac tgtgtaggtt ttgagcagtt cctgccgtat ctgctgggtg
      721 agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa actgaccggc attgatgccg
      781 aaaccattcg tgggctggcg cggcagatgg cggcgaacag aacgcaaatt attgctggct
      841 ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg gatgattgtg gttctggcgg
      901 cgatgctggg gcaaattggc ctgccaggtg gtggttttgg ttttggctgg cactacaacg
      961 gcgcaggcac gccggggcgt aaaggcgtta ttctgagtgg tttctccggc tctacgtcga
     1021 ttccgcctgt tcacgacaac agtgactaca aaggctacag cagcaccatt ccgattgccc
     1081 gttttatcga tgcgatcctc gaaccgggga aagtgatcaa ctggaacggt aaatcggtaa
     1141 aactgccgcc actgaaaatg tgtatttttg ccggaactaa cccattccat cgccatcagc
     1201 agatcaaccg cattatttaa ggctggcgca agctggaaac ggttatcgcc atagataacc
     1261 agtggacctc aacctgccgc tttgccgata tcgtactgcc tgcgaccacg cagtttgagc
     1321 gtaacgatct cgaccagtac ggcaaccact ccaaccgtgg cattatcgcc atgaaacagg
     1381 tggtgccgcc gcagttcgag gcgcgcaacg actttgatat tttccgcgag ctgtgccgtc
     1441 gctttaatcg cgaagaagcc tttaccgaag ggctggacga aatgggctgg ctgaaacgca
     1501 tctggcagga aggtgtacaa caaggcaaag gacgcggcgt tcatctgcca gcgtttgatg
     1561 acttctggaa taacaaagag tacgtcgagt ttgaccatcc gcagatgttt gttcgccacc
     1621 aggcattccg cgaagatccg gatctcgaac cgctgggcac gccgagtggc ctgattgaga
     1681 tctactcgaa aaccatcgcc gatatgaact acgacgattg tcaggggcat ccgatgtggt
     1741 ttgagaaaat cgaacgctcc cacggtgggc ccggctcgca gaagtatccg ttgcatctgc
     1801 aatctgtgca tccggatttc cgacttcact cgcagttatg tgagtcggaa actctgcgtc
     1861 agcaatatac ggtagcgggt aaagagccag tattcattaa cccgcaggat gccagcgcgc
     1921 gcggtattcg taacggtgat gtggtacgcg tctttaacgc tcgcggtcag gtgttggcag
     1981 gggcagtagt ttctgaccgc tatgcacccg gcgtggcacg aattcacgaa ggggcatggt
     2041 acgatccaga taaaggcggc gagcctggtg cgctgtgcaa atacggtaac cccaacgtgc
     2101 tgaccatcga catcggtact tcgcagctgg cgcaggcgac cagtgcgcac actacgctgg
     2161 tggaaattga gaagtacaat ggagcagtgg agcaggtgac ggcgtttaac ggccctgtgg
     2221 agatagtggc gcagtgcgaa tatgttcccg cgtcgcaggt gaaatcatga ccacgctgac
     2281 agcacaacag attgcctgtg tttacgcctg gctggcgcag ttgttctccc gtgaactgga
     2341 cgatgaacaa ctgacgcaaa tcgccagtgc gcagatggcg gaatggtttt cgttgctgaa
     2401 aagcgaaccg ccgctggctg cggcggtgaa tgagctggaa aactgtattg ccacgctgac
     2461 agtacgtgac gatgcccgtc tggaactggc cgctgacttt tgcggcctgt ttttgatgac
     2521 tgataagcag gcggcgctgc cgtatgcatc ggcctacaaa caggacgagc aagagattaa
     2581 acgcttgtta gttgaggcag ggatggaaac cagcggcaat ttcaacgaac cggcagacca
     2641 tctggcgatc tatctcgagt tgttgagtca tctgcatttt tcgctgggag aggggaccgt
     2701 tcctgcgcga agaatcgaca gtttgcggca aaaaacactg acggcgctgt ggcaatggtt
     2761 accagagttt gctgcgcgtt gccatcagta tgacagcttt ggtttttacg cggcactaag
     2821 ccagttattg ctggtgttag tggagtgcga ccaccaaaac aaataacgtc gtttatgcgc
     2881 ctgaaaagac gcgtttagcg tcgcatcagg cattatggcg cagttgccgg atgcgtcatc
     2941 acatccggca aacacacatc acggatgagc tacaaaccgg gaaagccgct ggcgtagcag
     3001 gcggttttcc tgcttcaggt gtgcaatatc atccattaac gtcagcgcca ccgcgatccc
     3061 cggccagtcc agagccagtt catgacgcag gcgtaccgcg cgttgcacca caatggcggc
     3121 atggtcgtca aatacccagg ttgtttcctg gatctcacgc ggttcaacca cccccaaacc
     3181 gacaatttca ttcaactcct cttcagagat gccggtatgc aggcaaaatt cggtaatagt
     3241 aaaagtcacc gtaacattag ccatcatgct ttcccccaat ctttacgtgg atcaaaagac
     3301 gactgggcgt ctgccagttg ctgccacagc gcggcagtgt tttcatccgg tttcggcggc
     3361 atcacgattt tcagtaccgc atacagatcg ccggtctgtt ttttgctcac cagacctttg
     3421 cctttaacgc gcaatcgttg cccggcctgg ctgcctggcg ggatagtcag caaaatgctt
     3481 tctttcagtg ttggaacggt gactttagca cccagcgccg cttcccacgg gctaaccggc
     3541 accacaattt ccagatcctg gccgacaata tcgaacagcg gatgtggcgc aatatgaatc
     3601 accagccaca aatcgccatt tggaccaccg ttttcgcccg gcgtcccctg gcctttcaga
     3661 cggatgcgtt gaccattgcc gacgcccgcc gggatcttca cattcagcgt tttcgggatt
     3721 tcctgttcga tcatgccaaa ggcgttataa accggcaggt tatagctgat ggtacgctta
     3781 tgctcagtca gcgtttcttc gaggaatacc gccacttcga tttcaatatc gtggccgcgt
     3841 gcggcggggc gttgacggct ctggcgggca tgctgaccga aaattgacga gaagatatcg
     3901 tcaaaatctt cggcattaaa actctgaccg tcgccatgat ggaactgacg gttaaattgc
     3961 ggatcgttgc gatgttgcca catctgatca tactcagcgc gacgttgttc atcacttaac
     4021 acttcccagg cttcagcgac ctctttgaag cgggcttcgg catccggttc tttgctgaca
     4081 tcaggatggt atttacgggc cagtcgacga taggcggtct tgattgtctt gagatcgtcc
     4141 gtcggtttca cgcccatgat ggcgtaataa tcctttaatt ccatagcgtt atctcgcgta
     4201 aatcaacaca aattgaagga acccctgtaa ggtaactcct ataagtgtag ggtaatcctc
     4261 aaaatttcat atgccaacac agaatatgtt attgaaatca tcgtggagag gaggtcgcca
     4321 tcaatatggg ttgctgaaca tattttaaac aggtgaaaaa gggtgagcga tttttgatag
     4381 ttgaaccagg cactttaagt ttaactaggg cgtcattatt tattaaattt t
//
LOCUS       DADLTD010000252         4412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLTD010000252 DADLTD010000000
VERSION     DADLTD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.256"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(62..724)
                     /gene="exoX"
                     /locus_tag="JN701_003764"
     CDS             complement(62..724)
                     /gene="exoX"
                     /locus_tag="JN701_003764"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY7366053.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLHWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(748..1404)
                     /locus_tag="JN701_003765"
     CDS             complement(748..1404)
                     /locus_tag="JN701_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY7366054.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(1506..1736)
                     /locus_tag="JN701_003766"
     CDS             complement(1506..1736)
                     /locus_tag="JN701_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY7366055.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            1875..2249
                     /gene="yobA"
                     /locus_tag="JN701_003767"
     CDS             1875..2249
                     /gene="yobA"
                     /locus_tag="JN701_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY7366056.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2253..3125
                     /gene="copD"
                     /locus_tag="JN701_003768"
     CDS             2253..3125
                     /gene="copD"
                     /locus_tag="JN701_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAY7366057.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHTIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLSALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            3138..3479
                     /locus_tag="JN701_003769"
     CDS             3138..3479
                     /locus_tag="JN701_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY7366058.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(3861..4331)
                     /locus_tag="JN701_003770"
     CDS             complement(3861..4331)
                     /locus_tag="JN701_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7366059.1"
                     /translation="MDLALVTGQRREDITNMRFSDIYDDRLHIRQIKTGMMIAIPLSL
                     SLPVAGLRLGAVVEQCRMVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKLTGIQ
                     FSENPPTFHEVRSLAGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         1097 a         1062 c         1089 g         1164 t
ORIGIN      
        1 cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct acgcctgcgg
       61 attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc atgctgtcca
      121 ggttattaaa tgaccagtgc agatagcccg gatcgcgttc ggcaacgtcg gaaaccgctt
      181 tgccacggta tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg gtgatatcgg
      241 ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac gcggcggtga
      301 tataacagtc atacaacgcg cggtgatgat gcaggcccgg cggcgtctgt acattgagct
      361 tccgtgtttt atataacgcc atattgctgt acttgatccc tggccacaaa cgacgggcca
      421 gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg tcaaagctgg
      481 cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct tcaatccacg
      541 gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt ggactaatag
      601 gacgatcggg gcgcaccagg tggctcatgg ggttgacgat ttttccgtca atgacatcaa
      661 cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg ataatgcgca
      721 acatggcatg ctccaggccg aaaaagccta gcgtaatgga atgatatcgc cttgccaacc
      781 ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa cgatgagacg
      841 accatattca tcccatagtg cactgttacc acgggcattt gccatcagca cggcaatcga
      901 gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg gttctcccag
      961 acattgccct gtagtaaaca gtgaacaggt cgggtccata tccatacctt gcggttgttc
     1021 atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac tctgatggta
     1081 gatcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa agcgatcgtt
     1141 atattcaacg ggaaggccgg caatgatggt cattcgccag gtcgttgcgg cataacaaag
     1201 cgggtctaac agtgagagat cgggtggggc aggaagggca cgtcgcgaat aatcacacgc
     1261 cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg cggccctcac
     1321 aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct catattgcgc
     1381 tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc aaattatggg
     1441 tgcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt aactctgtat
     1501 aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gtttgaccga agccaaacgg
     1561 tgggcaataa gcctctcgcg aaaccagctg cgcaaatgtt caggctgttc acgttcaacc
     1621 gcttcagcga tcaccggcat attgtagcgt tctttaaatg ccaccccggc cgccgccaaa
     1681 tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt cagcattctt
     1741 aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gccaggatgg caagccggaa
     1801 ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag ttattacaaa
     1861 aagtaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg gcaatcctga
     1921 ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat cagtatcctg
     1981 cggcaaacgc gcaagtgaca gctgcaccgc aggcaatcac tttaaacttc tcggaaggtg
     2041 ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat attaaaacat
     2101 taccggcgaa gcgaaatgag caggaccaaa agcaactgat tgttccactg gccgattcgc
     2161 tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg cataaaacga
     2221 aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg atcgcgctgc
     2281 gatttatcca tttcacctcg ctgatgctgg tttttggctt cgcaatgtac ggtgcctggc
     2341 tggccccctt gacgattcgt cgcttgctgg cgaagcgttt tctgcgttta caacagcacg
     2401 ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc gggttgatgg
     2461 gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg caaacgcagt
     2521 ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc gtcgccctta
     2581 tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa tttattctgc
     2641 tggcgggagt ggggcatgcg acgctgaatg aaggggtaac ggcgaaaatc catcagacta
     2701 atcacacgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg ccggtgctct
     2761 ggtgtatgca actcatcaaa ggccgttggc gacaccaggc tattcaggcg ctgatgcgtt
     2821 tttcatggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg cttagtgcat
     2881 tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc ttattgctca
     2941 aagccatcct ggtgatgatt atggtggtca ttgcgctggc taatcgttat gttcttgtac
     3001 cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg actaaactcg
     3061 aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact ctcgaacctt
     3121 tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta ctcaccagtt
     3181 ctgcagcggc gctggctgca ccgcaggtga ttaccgtcag tcgttttgaa gtgggtaaag
     3241 acaaatgggc gtttaatcgc gaagaggtga tgctgacttg ccgaccgggt aatgctttgt
     3301 atgttattaa tccgagtacc ctcgtgcagt atcctttaaa cgatatcgca caaaaggaag
     3361 ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat gatcctaaca
     3421 atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa ctctgttaat
     3481 tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt caccagaacg
     3541 ggcggtttta acatttcagc tgatgaccac cacgcttttt attgaccatt ttgcacgcaa
     3601 actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag cctgcattaa
     3661 tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg aatacaggaa
     3721 tcgtattcgg tcttttttta attgcatttg aaatcaatta gttgcaaatg tctccccgaa
     3781 atttactcga atttctgtat tccggtcttt tttggttata tcacaaccaa aatacattta
     3841 acaatccatt tacgttaaaa tcagagcagt aagtacgttt tttctctctc atcaagatac
     3901 atttttgttg tcttctccga tgtgtggcca agtagacgct gagcaaattc ttctccacat
     3961 gtttctttgt acaatcgtcc agccaggctt ctgacttcgt gaaaagttgg tgggttttca
     4021 ctgaactgga tacctgttaa ttttctggct gcgacaaatt tctttgtcag gccgtccggg
     4081 tgaatgctgc cgtcagggct gttttttcta atcccggcac tgattagata atccccccta
     4141 cttaccatgc ggcactgttc aactactgca ccaagtcgta aaccagcgac aggaaggctg
     4201 agtgacaggg ggatagcaat catcattcct gtcttaattt gcctgatgtg gagacgatca
     4261 tcataaatat cactgaaccg catattcgtt atgtcttcgc gacgttgtcc tgttacaagg
     4321 gctaaatcca tagctaatgg gaaccatgcc ggaagttgat ctgctgcctc cctgatgcag
     4381 ttgtatgtct gaggtagcct gagtttaacg ga
//
LOCUS       DADLTD010000253         4398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLTD010000253 DADLTD010000000
VERSION     DADLTD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.257"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..222)
                     /locus_tag="JN701_003771"
                     /pseudo
     CDS             complement(1..222)
                     /locus_tag="JN701_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            225..597
                     /locus_tag="JN701_003772"
                     /pseudo
     CDS             225..597
                     /locus_tag="JN701_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1159..2106)
                     /gene="icsP"
                     /locus_tag="JN701_003773"
     CDS             complement(1159..2106)
                     /gene="icsP"
                     /locus_tag="JN701_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY7366060.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            3068..3205
                     /locus_tag="JN701_003774"
     CDS             3068..3205
                     /locus_tag="JN701_003774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366061.1"
                     /translation="MTTTELFWDLNAIKWLVEGRGDDVSPFENIPVPDLTYNKEAWQF
                     G"
     gene            complement(3487..3639)
                     /locus_tag="JN701_003775"
                     /pseudo
     CDS             complement(3487..3639)
                     /locus_tag="JN701_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3695..4387
                     /gene="ospZ"
                     /locus_tag="JN701_003776"
     CDS             3695..4387
                     /gene="ospZ"
                     /locus_tag="JN701_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY7366062.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNESPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1353 a          863 c          849 g         1333 t
ORIGIN      
        1 gatctggttc aacaatatgc agatggtaca caacctcttt aaccagccca tccggattat
       61 tcagagccct tccgcaatcc ggaataaaag tgctgtttta ccgtgaaccc tctgaaaatc
      121 agcgagcagt gttttaacca gccgacgttc agagcgtact gacagacggt ggacaatttg
      181 aataaacagt tcaatcagac cgtagatgct ctcccgttgg cgacatgctg ggagcggtgg
      241 aacaccgctt cggcaacgag cttccggcgt ctccagtgaa gtgactgacg gataatggtt
      301 catgctaccg ggctaatgaa acacgccagt tcacccggat gttgggactt tgttgaaccg
      361 aagaacacgg cggtgcggag tccggagagt aacagaatag caaagagctt cgtgaaaaca
      421 ataaagtgtg actacatcag tatcatgccc aaacctgacg ggttaacggc agcaaagaac
      481 cttgcagagg cattcgagca ttataacgaa tggcatccgc atagtgcgct ggattatcgc
      541 tcgccacggg aatatctgcg gcagcgggcc aatgataaca ggtgtctgga aatatagggg
      601 caaagccacc cacgaacgat ccagcatata atcaagaaat tagatttcat ttaacataag
      661 atagattatg tgcaccaaga ggcatttcct taatttctga atttacaggc tcttttgctc
      721 tctttgctat gctgtggatt tggaaagata gagtgctggc tgattattta ggtattttca
      781 tcagcatgtc cctgatagca ctgttccatc agcgctttca ggcgataata gtagaccttg
      841 ggtagcctct gctcatgtgt aacttgatac cccgagaaca gccacgatga tctaagcggt
      901 tcttatttct ccggtctcag ggtcggtaat gccaacagta agtccacagt aatcaacgaa
      961 gagctttttg aaccgccccg gaaatcctgg agactaaacg ccctgagaaa gaggtaaaca
     1021 gaatgactaa aaatacacgt ttttcccccg aagtccgtca acgggcagtt cgtatggttc
     1081 tggaaagtca gggcgaatat gactcacaat gggcggcaat ttttagccat gaaaacgtaa
     1141 tcaactcggt gaaccaattc aaaaaatata ctttatacct gcggaagtga gaaaaccaat
     1201 atattcaata cttgcactat ttttaatggt gccagatata ttggtacttt gctcataaag
     1261 agatgtatca ccttttttat tagaaatgta atttctggag ccttctatat aaaattttgc
     1321 actgggggtg acatagtatc caatattcgc agcaacagag cagtaatttt ggttttttat
     1381 ttcatcttta aaaattgtct cagtctgata gtgtttatct gtatcagatg aacgaaccca
     1441 accactatat ttcagttctg ctccaaactc aaaattctca tggcggtaat tagcagttaa
     1501 tccaatataa ggtattttga agagctgttt gtaacctatt gttctttcac cactaggatg
     1561 tgcccctttt ttattcctgt tccccccgtt ttcactataa atataactcc ctcccattgc
     1621 attaaaactg taacgactct cttggtaacc tgctattagt ccaagtcgat aatccaaatt
     1681 atttaataac caacctttaa gattcagctc gaattcgtta gcatcacgga gatgcgtatt
     1741 gggatgccag ctctggtcgg tccatacctg aggggtattg gaattgttcc agtctttatc
     1801 aaccatgctt gctttctggt tacctaaagt agtccaaccg gaaactccga aagacacttt
     1861 tggaattagc ttccaatcga tgccacctct aacaatagtc gcattactgt atttccagtc
     1921 cagttgacta atttttctcc ccccttcctt gggatgataa acgcgttctt ttgttttgcc
     1981 actcagagat cctagactaa tatccgtact gatgttatcc ggtatgaaaa gtggatagtt
     2041 agtggtagca tgtgtagtaa agatcgcagg tacacaaagt gcaagtacaa ggaattttaa
     2101 tttcatcgag aactctactt ttttggttga aatgtccatg atcttcttac tttataagat
     2161 aaacgtcaat gccaagcatt attttaatta aatatttgtt tatgttacca agaacatatc
     2221 ttttattata atatttattg aacttttgta aaaataaaaa aatacctcat tgtgcgaata
     2281 aagtaacggg ggccatagat ttctctacct accttttggt gttcatacct gaggctgata
     2341 gtttcaggat tccgcgacaa ttctactcac cttcttgttc tctcgccaat atgaaatcaa
     2401 tcagaaaagc gttccataag aacaacgata agacaataaa ttgtcatcac ggctgcaata
     2461 gccaacgtgc ctttcagtac caacatgaca acctcctgta tttgacgtaa cgtgtgctga
     2521 taaatatgta tttgtatctc aactgactca atacaacact taagtatagt ttttcagatt
     2581 ttgtttttcc cgtgaaatat tatggaaatg gcagaagcaa tacattgaat tccgattcga
     2641 gtgaatacgg ctagcgggca cctcacttta gcactgaagc catcaacttt tatttgactt
     2701 atttcaatgg aaagctttct cactatcagt acaggtaatt cccatccttt taaagtcgag
     2761 agtcagactg cgactaatac agttaaagtg accaaactca gttatcctct acacatttta
     2821 cctcatgttt acttggacaa ttcattggtg cttttataaa agaataaata ttctactaat
     2881 ttaatgcatc acctaaaata gtatttttta taatagaact gtatctatag tatattatta
     2941 aagcttaatt atttattatg ctttaatgtg gttgtccgat taaggactaa tccttgaaaa
     3001 cgccgtatag ggcgggagtt cactctattg cgataacgta accgtacaat attgtgcgtt
     3061 tcccaaattg accactactg aactattctg ggatttaaat gcaataaaat ggttggttga
     3121 aggtcgtggt gatgatgtta gtccttttga gaatattcca gtgccagatc ttacttataa
     3181 taaagaggct tggcagtttg gataagctat tgtccttatt ccactaatac aaactttttg
     3241 taaagtatat taaatatact tcatttcata ctgaaatccc cggaagtatt caatcaaaca
     3301 attacaccat aaaaggaaga gggagcccca attaagttat caagcatgtt tatgatacct
     3361 gcagggtata aattttctgg ctgcaagcag acacatataa aacgtcttta ttgatcctac
     3421 ccacataata tggacgcagt cgtaagtgag gctctgtgag cctacccacg taatatggac
     3481 acaggcctaa gcgaggttct ggttttcaaa ttgttccgga ctgaggccgc cacaccaacg
     3541 gtgccgtcgc cagcgattgt aatcgcactc gatataatta aacactgttg cccgcactat
     3601 ttcccgatag ataaagtgtt ctccgtggag acattccact taggaacaat tatattgttc
     3661 atggtaatgt ttatatttga gtatagagat taaaatgatt agtcccatca agaatattaa
     3721 gaacgtattt ccaataaata ctgcaaatac tgaatatata gtgagaaata tttatcctag
     3781 agttgaacat ggttacttta atgaatcacc aaacatatat gacaagaagt atatctctgg
     3841 tataaccaga agtatggcac aattaaaaat agaggagttt attaatgaga aatccagacg
     3901 acttaattat atgaagacaa tgtattctcc atgtccggaa gattttcagc ctatttccag
     3961 agacgaagcc agtataccgg aaggaagctg gttgacagtt atatctggaa aacgtcctat
     4021 ggggcaattt tctgtggata gtttgtacca tcctgactta catgcattat gtgagctccc
     4081 agagattagt tgtaagatct tccctaaaga aaatagtgat tttttgtata taattgttgt
     4141 attcagaaat gaatctcccc aaggagaact acgggcaaat cggtttatag aactatatga
     4201 tataaaaaga gagattatgc aagtgttaag agatgagtcg ccagagttaa agtctattaa
     4261 atctgagatt attattgctc gtgaaatggg agaacttttt agttatgcat cagaggaaat
     4321 agatagctac ataaaacaaa tgaatgacag gttttctcaa atcaaggctc gaatgtctgt
     4381 tacttaatgt atattaat
//
LOCUS       DADLTD010000254         4398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLTD010000254 DADLTD010000000
VERSION     DADLTD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.258"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..796)
                     /locus_tag="JN701_003777"
     CDS             complement(<1..796)
                     /locus_tag="JN701_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAY7366063.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESTFGWQEAAGGAAGYTLSQAITNGFQ"
     gene            1075..2028
                     /gene="tal"
                     /locus_tag="JN701_003778"
     CDS             1075..2028
                     /gene="tal"
                     /locus_tag="JN701_003778"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7366064.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2143..2730
                     /gene="mog"
                     /locus_tag="JN701_003779"
     CDS             2143..2730
                     /gene="mog"
                     /locus_tag="JN701_003779"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY7366065.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2765..3331)
                     /gene="satP"
                     /locus_tag="JN701_003780"
     CDS             complement(2765..3331)
                     /gene="satP"
                     /locus_tag="JN701_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY7366066.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3480..4193)
                     /gene="msyB"
                     /locus_tag="JN701_003781"
     CDS             complement(3480..4193)
                     /gene="msyB"
                     /locus_tag="JN701_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7366067.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4219..>4398)
                     /locus_tag="JN701_003782"
     CDS             complement(4219..>4398)
                     /locus_tag="JN701_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY7366068.1"
                     /translation="ARSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHT
                     VNSSDMATLKIIGDD"
BASE COUNT         1157 a         1127 c         1108 g         1006 t
ORIGIN      
        1 gctgaaaacc gttagtaatc gcctggctta aggtatatcc cgccgcgccg cctgccgctt
       61 cctgccagcc aaaagtactc tcaaaaatag accaaatgac gtggggaagt tgcccgatat
      121 ttattacgca aatcaccatg ctggtcagca cccagattat cgccatcaac gggacaaacc
      181 cctgcatgag ccgggcgacg ccatgaagac cgcgagtgat tgccagcaga gcaaagacag
      241 cgagaataat gcctgtcacc agcgggggaa aatcaaaaga aaaactcagg gctcgggcaa
      301 cggcgttcgc ctgaactccg ctgaaaatta tgccgtaggc gatgagcaaa aagacggcga
      361 acagaacgcc catccaacgc atccccagcc cgcgcgccat ataccatgcc ggtccgccac
      421 gaaactgccc attgacgtca cgttctttat aaagttgtgc aagggaacat tcggcaaacg
      481 aggtcgccat gccgataaac gcggcaaccc acatccaaaa gacggctcta ggtccaccgg
      541 cggtaatagc cagcgcaacg ccggccaggt tgccgctacc cacgcgcgcc gcaagactgg
      601 tacacaatga ctgaaatgag gttaaaccgc ctggctgtgg atgaatgcta tttttaagac
      661 ttttgccaaa ctggcggatg tagcgaaact gcacaaatcc ggtgcgaaaa gtgaaccaac
      721 aacctgcgcc gaagagcagg taaatcatta ccgatcccca aaggacgctg ttaataaagg
      781 agaaaaaatc tggcatgcat atccctctta ttgccggtcg cgatgacttt cctgtgtaaa
      841 cgttaccaat tgtttaagaa gtatatacgc tacgaggtac ttgataactt ctgcgtagca
      901 tacatgaggt tttgtataaa aatggcgggc gatatcaacg cagtgtcaga aatccgaaac
      961 agtctcgcct ggcgataacc gtcttgtcgg cggttgcgct gacgttgcgt cgtgatatca
     1021 tcagggcaga ccggttacat ccccctaaca agctgtttaa agagaaatac tatcatgacg
     1081 gacaaattga cctcccttcg tcagtacacc accgtagtgg ccgacactgg ggacatcgcg
     1141 gcaatgaagc tgtatcaacc gcaggatgcc acaaccaacc cttctctcat tcttaacgca
     1201 gcgcagattc cggaataccg taagttgatt gatgatgctg tcgcctgggc gaaacagcag
     1261 agcaacgatc gcgcgcagca gatcgtggac gcgaccgaca aactggcagt aaatattggt
     1321 ctggaaatcc tgaaactggt tccgggtcgt atctcaactg aagttgatgc gcgtctttcc
     1381 tatgacaccg aagcgtcaat tgcgaaagca aaacgcctga tcaaactcta caacgatgct
     1441 ggtattagca acgatcgtat tctgatcaaa ctggcttcta cctggcaggg tatccgtgct
     1501 gcagaacagc tggaaaaaga aggcatcaac tgtaacctga ccctgctgtt ctccttcgct
     1561 caggctcgtg cttgtgcgga agcaggcgtg ttcctgatct cgccatttgt tggccgtatt
     1621 cttgactggt acaaagcgaa tactgataag aaagagtacg ctccggcaga agatccgggc
     1681 gtggtttctg tatctgaaat ctaccagtac tacaaagagc acggttatga aaccgtggtt
     1741 atgggcgcaa gcttccgtaa catcggcgaa attctggaac tggcaggctg cgaccgtctt
     1801 accatcgcac ctacactgct gaaagagctg gctgagagcg aaggggctat cgaacgtaaa
     1861 ctgtcttaca ccggcgaagt gaaagcgcgt ccggcgcgta tcactgagtc cgagttcctg
     1921 tggcagcaca accaggatcc aatggcagta gataaactgg cggaaggtat ccgtaagttt
     1981 gctattgacc aggaaaaact ggaaaaaatg atcggcgatc tgctgtaatc attcttagcg
     2041 tgaccgggaa gtcggtcacg ctacctcttc tgaagcctgt ctgtcactcc cttcgcagtg
     2101 tatcattctg tttaacgaga ctgtttaaac ggaaaaatct tgatgaatac tttacgtatt
     2161 ggcttagttt ccatctctga tcgcgcatcc agcggcgttt atcaggataa aggcatccct
     2221 gcgctggaag aatggctgac atcggtgcta accacgccgt ttgaactgga aacccgctta
     2281 atccccgatg agcaggcgat catcgagcaa acgttgtgtg agctagtgga tgaaatgagt
     2341 tgccatctgg tgctcaccac gggcggaact ggcccggcgc gtcgtgacgt aacgcccgat
     2401 gcgacgctgg cagtagcgga ccgcgagatg cctggctttg gtgaacagat gcgccagata
     2461 agcctgcatt ttgtaccaac tgcgatcctt tcgcgtcagg ttggtgtgat tcgcaaacag
     2521 gcgctgatcc ttaacttacc cggtcagccg aagtctatta aagagacgct ggaaggtgtg
     2581 aaggacgctg cgggtaacgt tgtggtacac ggtatttttg ccagcgtacc gtactgcatt
     2641 cagttgctgg aagggccata cgttgaaacg gcaccagaag tggttgctgc attcagaccg
     2701 aagagtgcaa gacgcgacgt tagcgaataa aaaaatcccc ccgagcgggg ggatctcaaa
     2761 acaattagtg ggattcacca atcggcagaa cggtgcgacc aaactgctcg ttcagtactt
     2821 cacccatcgc cagatagatt gcactggcac cacagatcag accaatccag ccggcaaagt
     2881 ggatgattgc ggcgttaccg gcaatgttac cgatcgccag cagggcaaac agcacagtca
     2941 ggctaaagaa aacgaattgc agaacgcgtg cgcctttcag cgtgccgaag aacataaaca
     3001 gcgtaaatac gccccacaga cccaggtaga caccaaggaa ctgtgcattt ggcgcatcgg
     3061 tcagacccag tttcggcatc agcagaatcg caaccagcgt cagccagaaa gaaccgtaag
     3121 aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta ctccagcaga ccagcaaaaa
     3181 tttgcgcgat gccgctgtag aaaatgccca tggcaagaat aataccgtcc agagcgaaat
     3241 aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc gaagcccatc aggcccagcg
     3301 gtgccggatt agccaactta gtgttgccca taattcctca aaaatcatca tcgaatgaat
     3361 ggtgaaataa tttccctgaa taactgtagt gttttcaggg cgcggcataa taatcagcca
     3421 gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa ttttccccgg tttccggtat
     3481 cagacctgag tggcgctaac catccggcgc aggcaggcga tttgcagtac ggctggaatc
     3541 gtcacgcgat aggcgctgcc gctgaccgct ttaaccccat ttagtgccgc acctacaggg
     3601 cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta cgctcattgc tgcgtgggtg
     3661 cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca cgccttttgc caataatttg
     3721 tctttcatca gcagcggcag cagctcttcc agctcattca ccctcgcatc gaacgcgtgc
     3781 agaaactcct gcttatgttc ctcgtccatt ttcttccagg tattacgcag aaattgttcc
     3841 agtaactgtt gctcaatttc aaacgtagac atctctttgt cggctttcag cttcaatcgt
     3901 tttgaaacat cgagcaaaat ggcccgatac aatttaccgt gtccgcgcag tttgttggcg
     3961 atactatcgc caccaaaatg ctgtaattct ccagcaatca gctgccagtt gcggcgatgt
     4021 tgctcgggat gcccttccat cgatttaaac agttcgttgc gcatcagtac gctggagagg
     4081 cgagttttgc ctttttcatt atgggtgagc aatcgggcga aatttgccaa ctgttcctca
     4141 ctacaatgct gaagaaaatc cagatctgaa tcattcaggt aattaacatt cattttttgt
     4201 ggcttctata ttctggcgtt agtcgtcgcc gataattttc agcgtggcca tatccgatga
     4261 gttcaccgta tgaccggaaa aggtgatttt tgagacgcag cgtttattgt cgttatcact
     4321 gttaatgttg atccagtcag tggtttgccc ttcttttatt tctgaaggaa tattcagact
     4381 ctgactggcg ctacgggc
//
LOCUS       DADLTD010000255         4373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLTD010000255 DADLTD010000000
VERSION     DADLTD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.259"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..100)
                     /locus_tag="JN701_003783"
     CDS             complement(<1..100)
                     /locus_tag="JN701_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY7366069.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVE"
     gene            complement(113..1894)
                     /gene="gcl"
                     /locus_tag="JN701_003784"
     CDS             complement(113..1894)
                     /gene="gcl"
                     /locus_tag="JN701_003784"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY7366070.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLVSDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTYFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            complement(1984..2799)
                     /gene="allR"
                     /locus_tag="JN701_003785"
     CDS             complement(1984..2799)
                     /gene="allR"
                     /locus_tag="JN701_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY7366071.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2877..3359)
                     /gene="allA"
                     /locus_tag="JN701_003786"
     CDS             complement(2877..3359)
                     /gene="allA"
                     /locus_tag="JN701_003786"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY7366072.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGYDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3589..>4373
                     /gene="allS"
                     /locus_tag="JN701_003787"
     CDS             3589..>4373
                     /gene="allS"
                     /locus_tag="JN701_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY7366073.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPT"
BASE COUNT         1118 a         1184 c         1044 g         1027 t
ORIGIN      
        1 attcaacgcc gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga aaatcatatt
       61 caccaaataa catcgataaa ttagcagaga aacgtaacat tatttatctc ccttattcat
      121 agtgcatgaa gcaggtttca gtcggtgcgt ccactgcgtt atcggcgata tcttcaaatt
      181 ccatgacgtt atccagttcg ctgcccatcg aaatattggt cacacgctcg agaataactt
      241 ccacgactac cggtacccga tattgcgcca ttaaggcttt cgcctgttca aaggctggcg
      301 caatatcttc cggtttgaag acgcgaatcg ctttacaacc taaaccttcc gctactttta
      361 cgtggtcgac gccgtaaccg ttcacttcgc tggagttgat attctcgaaa gcgagttgca
      421 cgcagtagtc catgtcaaag gcgcgttgtg actgacgaat caggccgaga taggcgttat
      481 tcaccagcac atggatgtac ggaatgttga actgcgcgcc aacggctaac tcttcaatca
      541 ggaactggaa gtcaaagtcg ccagaaatcg ccaccacatt gcgtttcgga tcagcggcac
      601 aaacccctag cgcagccgga atcgtccagc ctaacggacc agcctgacca cagttgatcc
      661 agtggcggtc tttaaagaca tgcagcattt gtgccgcagc gatttgtgac agaccgatgg
      721 tggtgacata acaaacatcg cgaccaaagg ctttgttcat ctcttcatac acgcgctgcg
      781 gtttcaccgg cacgttgtcg aagtaggttt tgcgcagcaa agtgcgtttg cgctgctggc
      841 agtcggcgac ccattcttta cgacacggca gacgacccgc tttttgcatc tcctgcgcca
      901 cttcaaccag cagtgtcagc gccgctttag catcagagac aatgccgaga tccggacaca
      961 gcacgcgacc aatttgcgtc ggctcaatat cgatatgaac gattttgcgc ccttcggtgt
     1021 atttctctac cgaaccggta tgacggttag caaaacggtt accgatacca aacaccatgt
     1081 cggacaccag caacgttgcg ttaccgtaac gatgcgcggt ttgcagaccg accatcccgg
     1141 ccatcagttc atgatcgtcc gggatacagc cccagcccat cagcgttggg atcaccggaa
     1201 cgctggtcag ttcagcaaac tgttgtaaca gtgcagctgc gtcagcattg attaccccgc
     1261 ccccggcaac aatcaccgga cgttcggcct ggattaacat ttctacagct ttttcgatct
     1321 gcatacggct ggcagcaggt ttgtagaccg gcagcggttc gtacatgtca ggatcaaact
     1381 cgatttccgc aacctgaacg tcgaacggta aatccaccag taccggaccc ggacgaccag
     1441 aacgcatcag gtgaaatgcc tgttgcagca cgcgaggcac cagcgccgct tcacgaactg
     1501 taaccgccat tttgctgacc ggtttagcaa ttgcttcaat atctacggcc tgaaaatctt
     1561 ctttatgcag acgggcgcgc ggtgcctggc cggtaatgca cagaatagga atggaatcag
     1621 cagaagcgga atagagcgcg gtgatcatgt ccgtgcccgc aggaccggaa gtccccagac
     1681 atacgccgat attccctgcc gttgcgcggg tataaccttc cgccatgtgc gaagcacctt
     1741 ccacatgacg cgccagaatg tgacgaatac cgccgtgctt acgcatcgct gagtagaacg
     1801 gattgattgc agctcccgga acaccgaagg cggtggtgat accttctttc tccagcacat
     1861 acattgccgc gtcaacggct ctcatttttg ccattttatt cctatctcta tttattggaa
     1921 aatttttcca actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg gatgcgacag
     1981 acattatgga tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg tgtctctgac
     2041 cagctcaccc tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc cggagatgga
     2101 gatagcggca acaacgctac cgacatcatc gtaaattgct gaagctatgc aattcagacc
     2161 tacaacatgc tcttctttat ctacggtata gcccagttca cgcgcttgtt ccaggtcctt
     2221 cagcaaggtg ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac cggtttgcag
     2281 aatgatgctc atcaactcct cttccgccag cggataaagc agcgctttgc ccgcaccgga
     2341 agcatgcagc ggcagacgac tgcccagtgg cgcacacatc ctgaccattg atttacactc
     2401 taactgacca attaatactg cttcattgcc gttacggatc gcgacattga ccgtttcgcc
     2461 ggaaagtaac atcaggcggc gcataaacgg cccggcgacg gagaggacat cgcggttatg
     2521 gatgtacgcc gcaccgacgt taaagacacc taatcctata tgccaccagc ctaattgact
     2581 gtcctgatag acaaaatccg ctgcttgtaa aaccttcagc aagcgaaagg tcgtggagag
     2641 cggcaaatcc agattgagag aaatatcgct aaccgacgaa cttcccccac ttttttccag
     2701 atattgcaga atcgcaattc cccgctctaa cgcctgtgcg cccttctgtg ccacaggctc
     2761 cgcctgtcct ggcctgccgc gccgtctaac ttccgtcata cctacgttaa ctccgaccta
     2821 caagtgagca taaacaatga acaattagtt tatcttatgc aagtcggttg caggcgtcac
     2881 gcaaaacaga gttcctgttc aggaatactt tcaacatcac agttgtcact gccgccgcga
     2941 tcgatggtca gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg ccagacgtta
     3001 cgatggtaat tcactccctg ttcgccgttg gtgataaacg cccgcagcgt tgacaggtct
     3061 ggtttgtcgt agcctaacgc cacgaccacc acaaacactt cacctttcat cgggataaag
     3121 gcctgagtgc ccagcggatg acgttcgagt tcgtgaatgg tcagcggcag attcgccggt
     3181 tgcgcgcggt taatgctaat aagcgtacgg tcttgctcaa gaatctcaac cagcgccaaa
     3241 tcgtggtaac gctccaccag gccattgtta atatggaaaa aatcccgttg ctgagtttcg
     3301 attacgtcgc cataagcact aaaggcttcc tgacttaacg gtaatacctg aagtttcatc
     3361 tttttgtcct tgtaacataa ttccgttcaa ccaccgccga ccggtcagct tgggttcaat
     3421 aacaggcaat ctaaaacctt aagagaacaa catcaataca aaagttggaa tatttttcca
     3481 aaactagatt tagtccacaa attgaacagg gttatcatgt aaaaatactg ataaatcaga
     3541 ggataagaaa atcacgcaac ttgcggattt aaaaggggat acctgaagat gttcgatcca
     3601 gaaaccttgc ggactttcat tgcggttgct gaaacaggaa gtttttcaaa agcggcagaa
     3661 cgattatgta aaaccacggc gacgatcagt tatcgcatta aacttctgga agagaatacc
     3721 ggagtagcgc tgtttttccg tacgactcgt agcgtaacgt tgacagcggc tggcgagcat
     3781 ctactttccc aggccagaga ctggctgagc tggctggaaa gtatgcccag cgagctgcaa
     3841 caggtgaatg atggcgtgga acgccaggtg aatattgtca tcaacaacct gctctataac
     3901 ccccaggccg tcgcccagtt gctggcgtgg ctgaatgaac gttacccctt tacccagttt
     3961 cacatctccc gacaaatcta tatgggcgtc tgggactcgc tattgtacga aggtttttcg
     4021 ctggctatcg gcgtcacggg aactgaggcg ctggcaaata cctttagtct tgatccctta
     4081 ggatcggtgc aatggcgatt tgtcatggcg gcggatcatc cgctggcgaa cgttgaagag
     4141 ccgctaacag aagcgcagtt gcgtcgcttt ccggcggtca atattgaaga cagcgcccgc
     4201 accttaacca aacgcgtcgc ctggcgattg ccagggcaaa aagagattat tgttcctgat
     4261 atggaaacga aaatcgccgc ccatctggcg ggcgttggca ttggtttttt gccaaaatcg
     4321 ctttgccagt caatgatcga taatcaacaa ctggtcagcc gggtaatccc aac
//
LOCUS       DADLTD010000256         4354 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLTD010000256 DADLTD010000000
VERSION     DADLTD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.260"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            155..1193
                     /locus_tag="JN701_003788"
                     /pseudo
     CDS             155..1193
                     /locus_tag="JN701_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490532.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1407..2555
                     /locus_tag="JN701_003789"
     CDS             1407..2555
                     /locus_tag="JN701_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7366074.1"
                     /translation="MFEIDSLLLITSVIILSLLAVKLFDEISPIQLVDHGRNNQIDGM
                     RGFLAIFVLIHHAAIWHGYLLTGVWKTPSSNLLTNLGQVGVSFFFMITGYLFFSKIRS
                     SDQDWVRLYISRFLRLTPMFIVSLCLVLLVIGFKSRWSVHVSPASLTVSLMRWAPFTA
                     LGMPNINGVKDSFTINAAVTWTLVYEWFFYFSLPVIAALFKRRVSIHMIIISVLVLVI
                     FLCYPSKKIHVISFLFGLLASYSNNYVHRVAKAKITPIMIIALLSYEMIYFPTTYAPL
                     PLIICGVCFILIASGCDFYGILRLNLTRKLGETTYSIYLLHGVFLYCVMTWVIPSNYS
                     NYFFIMLISITAFCVTLLSCITFKLIELPFINITKQTAMKIRGIIKNT"
     gene            complement(2835..3446)
                     /locus_tag="JN701_003790"
     CDS             complement(2835..3446)
                     /locus_tag="JN701_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7366075.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASINLIQLKLQAG
                     RNLTQAETSRLNTVLDYIDAVTATDTSTAPDVIWPELLDAHNM"
     gene            complement(3446..4228)
                     /locus_tag="JN701_003791"
     CDS             complement(3446..4228)
                     /locus_tag="JN701_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7366076.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTILKQITPLSPSIDF
                     DNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
BASE COUNT         1223 a          982 c          902 g         1247 t
ORIGIN      
        1 ttttcgcagt tccacagcca ccctgatacc attttattta aaaccgacat actaaatagc
       61 ttcgcaaaat agtagatcac gtaaaaggaa ctcagcccgg attgagcgat ctgatcaatc
      121 gacaaatatc ccaaaccacc aaccggactg agcgatgccg atcatagcag taactcctga
      181 tgaagaacga caactgatgc gcaaagaaac ccagcaaact catgataaga accatgtccg
      241 acaacttgtc gctatgctga tgctacatca gggaatgacc gtcaccgacg ttgccagact
      301 gctctgcgct gcacgctctt ccgtcggcaa atggataaac tggtttacct tgcatggcgc
      361 tgaaggatta aaaaacctta ggcccggacg tgccctacac tggccggtcg ccgatattct
      421 tcatattttg ctactgttgg tacatcgctc cccgaaggat tttggctggc tgcgctcacg
      481 ctggagcaca gaactgctgg cactcgtcgt caaccgacgt tttgacatga cgctgcatcg
      541 taccgcgctg cacagatacc taaaacaggc agatatagtc tggcgcagag cccgcaccaa
      601 cgctgaaaat caaagacccg cactatgatg aaaagcacct tgctattgag caggcgctga
      661 ctcaggggca gacgaaacat cctgtgtttt atcaggatga agtcgacatc gacctgaata
      721 cgaaaatcgg cgtggactgg atgcccaaag ggcaacagaa acgcatcgct acgccgggac
      781 agaacctgaa gcattatctg gctggcgcac tgcactcagg tacgggacgg gttcactagg
      841 ccagtggcag cagcaaaagt tctgatttat ttatcagtct gttagaggca ttacggcgta
      901 gatatcggcg ggcgaaaacc atcacactgg tggcggataa ctacatcatc cataaaggcc
      961 gtaaagtgga gcgatggctg gaagaaaacc cgaagttccg gttgctgttc ctgccgacgt
     1021 attcaccgtg gctgaatccg atagagcaac tttggttatc gttacacaag accataacgc
     1081 gaaatcatca gtgccggtat atgtggcaga tactgaaaca ggtaacgcag tttatgaatg
     1141 ctgctttacc gtttccgggt aatcaacacg gtatggccaa agtggagcgg taatatgcga
     1201 agctatttag attcagtttg aatctcagcc ctgacattca ttctttaccg acgttccatt
     1261 agcccaaatc tgagtatata acctcaattc aactgactaa atttaacaca atatcaatga
     1321 caaatgtctt taccattggt acatacaatt gcaaaatcac catctctaag caataagact
     1381 acgcatatta aagtgagtta tattaaatgt ttgaaattga tagcctatta ttaataacat
     1441 ccgtgataat cttgtcatta ttggctgtaa aattatttga tgaaatttct cctatacaac
     1501 ttgtcgatca tggaagaaat aatcagatag atggaatgag aggattctta gccatcttcg
     1561 tacttattca tcatgccgct atttggcatg gctacttatt aaccggggta tggaaaactc
     1621 cgtcatcaaa cttattaaca aatttaggcc aagttggtgt ctcattcttt ttcatgatca
     1681 ccggatatct atttttttcg aagattcgct caagtgacca ggattgggta agactatata
     1741 tatcgagatt tctgcgatta actcctatgt ttattgtaag tttatgttta gttcttcttg
     1801 ttataggttt taaatcaaga tggagcgtac atgtctcccc tgcctctctt actgtgtcac
     1861 tcatgagatg ggcaccattc accgcattag ggatgccaaa tattaacggt gttaaggact
     1921 cattcactat caatgcagct gtaacatgga ccctcgttta cgaatggttt ttttattttt
     1981 cattaccggt tatagctgca ctcttcaaaa gaagagtcag catccacatg attataataa
     2041 gtgttttagt tctagtgatt ttcttatgtt acccatcaaa aaaaatacat gttatttcat
     2101 ttttatttgg actgttagcc tcttattcaa ataattatgt acatagagtt gctaaagcta
     2161 aaataacgcc tattatgata attgcattac taagttacga aatgatatat tttcctacaa
     2221 cctacgctcc actccctctc ataatttgcg gtgtttgctt tatacttatc gcatcaggct
     2281 gtgattttta tggtatattg agattaaatt taacaagaaa acttggtgaa acaacatata
     2341 gtatttatct tttacatgga gtattccttt attgtgtaat gacatgggtc atccctagta
     2401 attattctaa ttattttttt attatgttaa taagcattac tgctttttgc gtcacacttt
     2461 tatcatgcat aacatttaaa ttaattgaac tacctttcat taatataaca aaacaaaccg
     2521 ctatgaaaat caggggcata ataaaaaaca cttagtaatc aattgtcacc taatcaagag
     2581 gtggtttata gataacaata aagaaccttc gccatttccg gaggttcttt tatatcctta
     2641 tggtaaatat aaattataaa tattattttt atggtgttcc actatgaaca gggaagctaa
     2701 ataatgatta caaaaatatg taacttcagg caatgcaggg gctatgttat tgccttaaca
     2761 ccataatgag taatattgtg tcccgaggat aaaagtactg ttaattctta ttgatgcttc
     2821 aatagcactc aaaatcacat attatgtgca tccaacaact caggccagat gacatccggc
     2881 gcggtgctgg tatctgttgc cgtcaccgcg tcaatgtaat ccagcaccgt gttaagtcgg
     2941 ctggtttctg cctgcgtcag attccgtccg gcctgtaatt ttagttgaat cagattgatg
     3001 gaagccattg cagtatcaat cagcgactgg cgttgtgctt ctgctgcatc gactgcggcg
     3061 ctatgttgtg cctcggtatc cgtcacccat ttctcaccat cccatttatc gtatggtgtt
     3121 aacgggacga tagtggttgt attatcaggg taatcacccg gagctgtgat ttcttttgat
     3181 tctcctgttt cggtgctaaa gacgatttca ccgcgatggt ctggcacata ttcccatgag
     3241 ttaaaatctg cagaacggca gattgcataa ccagctttgt atgcgcctgg agcatctaaa
     3301 caggaatatg ccggaatgcc gacaccaata gcaagatatt cagttgaagc tgaaatatac
     3361 tcccgactca tgacatcaaa gttataaaca gtaatttctc ctgccatagt ggcaattaat
     3421 tcactgttta attctgcgtt attcattatg cagccctcac aatatagtta aaagcgacat
     3481 ttcgtgggcg tgtttcattc gcacctgcag gtttagtcgt tgccgcaaca acaccaccct
     3541 ctgtaatagc cgggttggga attgggtaat tatcgaagtc aatggacgga gacaggggcg
     3601 tgatttgttt taaaatcgtg gtgcctgtaa aactttccag agtctggtcg ttagttcgtg
     3661 aagtccagat tccccaatag tggttatgac tgacaatagc accgtcctga agcgttaata
     3721 atccccttcc gttatccact ccacgcccgt catcccagcc ccgaataaac tcaccgcgta
     3781 aatcaggtaa tttatttgtc ggataagcct ttgccagttc cgggtattct tcagcagaaa
     3841 aagctgctcc gttgcatttc agccagcctg ttggcggagt ggctgaaggc cacggaacag
     3901 gcacaccaac gggtaatgct gagccttctc ccaaaccaag gttttcgaga gctgttttca
     3961 ccgtgccatc cgatttgata tcgccaaacg gattcttgcg gcttaacagc agcgcacgaa
     4021 gcgcggtaag cagctggtca tgccgcccct tctccaggct ggcaccggat gcctccacaa
     4081 cgctgcaaag ctcctcctgc aacatgtcaa agtagtcatc atccagatcg gtggcaggcg
     4141 tgccggtctg ggggttacca cgggtaaaac cgttcttacc cgcgccgaac ttatccttct
     4201 gcgcggtttt cgtgtctata cgatgcatgg attactccgg atatttaaaa atcacatagg
     4261 tatgcgacgg gcagagtttg ttaagaacac actcgacaac ggtgtccccc cagaagcgca
     4321 gcgcggaatc acagggatcg ccacatgtca tcca
//
LOCUS       DADLTD010000257         4320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLTD010000257 DADLTD010000000
VERSION     DADLTD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.261"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..976)
                     /locus_tag="JN701_003792"
     CDS             complement(<1..976)
                     /locus_tag="JN701_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HAY7366077.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEV"
     gene            complement(1131..1757)
                     /gene="dsbA"
                     /locus_tag="JN701_003793"
     CDS             complement(1131..1757)
                     /gene="dsbA"
                     /locus_tag="JN701_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY7366078.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1774..2760)
                     /gene="srkA"
                     /locus_tag="JN701_003794"
     CDS             complement(1774..2760)
                     /gene="srkA"
                     /locus_tag="JN701_003794"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY7366079.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2837..3106)
                     /locus_tag="JN701_003795"
     CDS             complement(2837..3106)
                     /locus_tag="JN701_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY7366080.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3176..3760
                     /gene="mobA"
                     /locus_tag="JN701_003796"
     CDS             3176..3760
                     /gene="mobA"
                     /locus_tag="JN701_003796"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY7366081.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3742..4269
                     /gene="mobB"
                     /locus_tag="JN701_003797"
     CDS             3742..4269
                     /gene="mobB"
                     /locus_tag="JN701_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY7366082.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1169 a         1021 c          966 g         1164 t
ORIGIN      
        1 caacttcatc gtttgctaac tcagggtgtg cagcaagctg cttctgcatc gcaatgttat
       61 actggatgat aaattggcgc actgctgagg gatcaataga ttcagcaatc aatgaccact
      121 caccactacc gagttgctga ttcccttttg ttaacttatc aagttcgtag gtcagattga
      181 cattgatata atctttgtct ttaacgcgat ccagcctggt ttttgcaacg aaagcatcga
      241 tttgggcaac gtcttccccc tgattgataa tattaacctg attaaatttc gactcgcttt
      301 caccaaccgg aaatttcttt tcttctaatc gtgtcagaga atcaatggta aaactttttg
      361 ccgttacttt gttcattggt gataattgca gaacaatatc ttcgactttg ccttcaatat
      421 ccaggttaga caatgaacta ccatcaccat taaaagtaaa ctggccctca ccaaaagaaa
      481 actttccata ctctgcaggg gcaacatcta atgtcgtaga gaatgagcca ccaaacgcga
      541 atcgcaatgt cgcttccacg ggcgatttat ttttcgcggc gataaatagc ggttgtgtca
      601 gttcattatt cactaagttc acttttgccg ctgccagtgc tgggatgaca ttaccatgca
      661 ttaacattgt gatgggcaac ggaccgtgct caacatccac atcaaatgca actttctggc
      721 ctggcttgat attgagatcg ggtgcaccgt tatcaaatga aatttgcatt tgaaaatggg
      781 aattaaaaaa gcctttgtca aagtttttat aacttaacgt catatcgtag gcatgttcac
      841 ctttcttttt agcatcgtta aaatctttaa taaatttttc gacaccaggc tgaatttgcg
      901 tacctgtgta ccatatgcca ccaccccaga ttacggctaa cgcaacaata acacctgtag
      961 ctgacttacg tatcatataa atattccctg ataaaacagc aaaactttga atatccactt
     1021 atgctgaaat aatgtccagc ggcaggatgt attatcagaa tgtatttata tttattaaat
     1081 aataaaaaaa gcccgtgaat attcacgggc tttatgtaat ttacattgaa ttattttttc
     1141 tcggacagat atttcactgt atcagcatac tgctgaacaa aaacatccat attgctggta
     1201 tccataccct gcggattcag ctgatattta ccgttaacaa acatcgccgg aacaccgcgc
     1261 aattgcacgt cagctgcagc tttttcctgc tgagctacca gagatttcac cacgaagctg
     1321 ttccacgccg cgtcgtactc ttcaccttta atacctgcgt tgataaatac atcgcggata
     1381 tcagaagcag aacgaatggt ctgggttttc tgtacgcctt caaacagcgg aacagtcact
     1441 ttgtcttcca cgcccagcgc catcgccaca gcccatgcct gagtcagatc tttgcccagg
     1501 tcaccaccca tgaagttgac gtggtattta gtcatcttca cgccttccgg cagttttttc
     1561 ttcacattat cagaaatatg cagaacttct tcaaactgat agcagtgcgg acagaagaaa
     1621 gagaaaaact ccagcacttg cggcgcgcca gctaccggtt tttccagggt agtgtactgt
     1681 ttaccatctt catactgcgc cgccgatgcg ctaaacgcta aaactaaacc agccagcgcc
     1741 agccaaatct ttttcatgat ctactctctc cgattaatac ataggtgtta attgtaaagg
     1801 gggttcttgt agaacttttg cctgttctat aaaagtcgcc gtttgtcgca gccagtaatc
     1861 ttccccggtt aaccacggga aatttttcgg gaacgcggga tcagcccaac gccgcattag
     1921 ccaggcaaga taataaacca aacgcatggc gcgtaaaggt tcaatcagtc cgatttcagc
     1981 ggtgtcgaac tcgctaaatt cttcataagc ttcaataata gtttccagtt gcatccgctg
     2041 ctcggcttta tcgccattga gcaacatcca caaatcctga atagctggac cattacgtgc
     2101 atcatccaga tcaacaaaca ttggaccatc gcgccagaga atattcccgg cgtggcagtc
     2161 tccatgtaag cgcagaacgg tggaatcttc ccgccagtgc gctgtaacgg cggcgatcag
     2221 ctcatctgtc gctttcagga atgccgcttt caacccggaa ggtatcagtg tagcgtcctc
     2281 aaacagcttg cgtggctcaa tgagatattc attcagaccg atggtcgggc gatggataaa
     2341 aagctgtttg cgccccgtct ggtgcatacg ccctaaataa cgcccaaccg cttccatctg
     2401 atcgatatta tcagcttcga actggcgacc accgacgctt ggaaaaacag cgaaataaaa
     2461 tccctgatga ttcaataaag tctgaccgtt aaaggccaca ggtgctgcga ccggaacttc
     2521 atcatttacc aactgcaacg caaattgatg ttcttcgagg atttgatcgg ctgtccaacg
     2581 ttcagggcga taaaatttga cgacaatacg tcgacgatct tcgtcctgaa attgatagac
     2641 acggttttca tagctgttaa gcggggtaag accggaatcc acccggatcc cttgctcaaa
     2701 tagagcatcc atgatggtat ccgggtgtag tgtctggaaa gtaaaagcgc tgttgttcat
     2761 cccatcatcc ggaaaacatt acgaatgatg aaggatatca tttcacgatg attttggtgg
     2821 cgccgcttac aagcttttac tctttaatta cgccgcgagc gcgtaacagc gccgttttga
     2881 agtcttcttc ataatctttc tgcaaacccg ggatcaccgc atctttcgcg gaatcacgca
     2941 ttttcaggtg atagatcaga atgtcatccg tcaaatccgc cagttcgccg tcaaaacctg
     3001 actctttcgc cagtttctgc aaaaattgca acaggttaag gtctggctct ttttgccagg
     3061 ctggctggag gaattcaata acttcattca gacgtttaca tttcatagtg atgctcctta
     3121 ctcttgagac agacacgtta gcagggtcaa tcccacaata aaagaggcga tatcggtgaa
     3181 tctgatgacg acgataacag gcgttgtgct ggcaggcggt aaagccagac gaatgggtgg
     3241 cgtagataaa ggattgcttg aattaaacgg caaaccatta tggcaacatg tcgctgacgc
     3301 gcttatgacg cagctctctc acgtcgtggt taatgccaat cgtcatcagg aaatctatca
     3361 ggcaagcggt ctgaaagtga ttgaagattc actggcggat tacccaggcc ctctggcagg
     3421 aatgctttca gtaatgcagc aggaagctgg tgagtggttt ttgttttgcc cgtgcgatac
     3481 gccttacatt ccccctgatt tagcagcccg gcttaatcat cagcgcaaag atgcgcctgt
     3541 cgtgtgggtc catgacggtg aacgcgatca cccgactatt gctctggtaa accgcgctat
     3601 tgagccttta ttactggaat atctgcaagc aggagaacgc cgggtaatgg catttatgcg
     3661 tctggctggc ggtcatgcgg ttgatttcag cgatcataaa gatgcatttg ttaacgtgaa
     3721 tacgccagag gagcttgccc gatggcagga aaaacgatga taccgttact cgcctttgcc
     3781 gcgtggagtg gtaccggaaa aactacgctt ttgaaaaaac tgatcccggc attatgcgcc
     3841 agagggatcc gtccagggct gattaagcat acgcaccatg atatggatgt tgataagcca
     3901 ggcaaagata gctatgagct gcgcaaggct ggcgcggcac aaaccatcgt tgccagccag
     3961 caacgatggg ccttgatgac ggaaacacca gacgaagaag agctggatct acagtttctc
     4021 gcaagtcgaa tggatacctc aaagctggat ttgattctgg tcgaagggtt taagcatgaa
     4081 gagatcgcaa agattgtgct gtttcgcgat ggagccagac atcgacctga agaattagtg
     4141 atagacaggc atgttattgc tgtagccagt gatgtgccgc ttaatcttga tgtcgcatta
     4201 ctggatatta atgatgttga ggggctggct gattttgtgg tggagtggat gcaaaagcag
     4261 aacggataag caagagtagg ctggatcaga cgcagccagt gtcgcatccg acctttttct
//
LOCUS       DADLTD010000258         4294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLTD010000258 DADLTD010000000
VERSION     DADLTD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.262"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            47..2209
                     /gene="ybiO"
                     /locus_tag="JN701_003798"
     CDS             47..2209
                     /gene="ybiO"
                     /locus_tag="JN701_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY7366083.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2269..3195)
                     /gene="rlmF"
                     /locus_tag="JN701_003799"
     CDS             complement(2269..3195)
                     /gene="rlmF"
                     /locus_tag="JN701_003799"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY7366084.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3471..3730
                     /locus_tag="JN701_003800"
                     /pseudo
     CDS             3471..3730
                     /locus_tag="JN701_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            3995..>4294
                     /locus_tag="JN701_003801"
     CDS             3995..>4294
                     /locus_tag="JN701_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7366085.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          999 a         1130 c         1075 g         1090 t
ORIGIN      
        1 agcatctata cttatctttt tgctctgttt tctcactgga ggagtcatgc ggtggatcct
       61 gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg gtatccatac ccggcgttac
      121 aaccacaacg acaacggact caacgactga accggccccg gaaccggata tcgaacaaaa
      181 aaaagcggcc tatggcgcac tggcggatgt gctggataac gacacctcgc gtaaagaatt
      241 gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg gaaccggtcc cgaagatcgt
      301 gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag gtcaccgaag tcagccgcca
      361 ttatggtgaa gccctttccg cccgcttcgg gcaactttat cgcaatatta ccggctcccc
      421 gcataagccg tttaatccac aaacattcag caatgcgctg acccattttt caatgttagc
      481 ggtattagtg tttggttttt actggctgat tcgcctgtgc gcactgccgc tgtatcgcaa
      541 aatgggcctg tgggcgcggc aaaaaaatcg tgagcgcagt aactggttgc agcttccggc
      601 gatgattatc ggggcgttta ttatcgacct actgttactg gcactgacat tatttgtcgg
      661 ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc atcgctttcc aacaaagttt
      721 gtttctcaac gcctttgccc tgattgaatt tttcaaagct gtactacgcc tgattttttg
      781 cccaaacgtg gctgaactgc gcccgttcac gattcatgac gagaccgccc gttactggag
      841 tcgtcgcctg agctggttaa gcagtttgat aggttatggc ctgattgtgg ccgtgccgat
      901 tatctctaat caggtgaatg tacagatagg tgcgctggcg aatgtcatca ttatgctgtg
      961 catgaccgtc tgggcgttat acctgatctt tcgcaataaa aaagagatta cccaacattt
     1021 gctcaacttt gcggagcatt cgctggcctt tttcagcctg tttatccgcg cctttgcgct
     1081 ggtgtggcac tggctggcaa gcgcctattt tatcgtgctg tttttctttt cgttgttcga
     1141 tccgggcaac agcctgaaat ttatgatggg tgcaacggtg cgcagcctgg cgattattgg
     1201 tatcgcagcg tttgtttccg gtatgttttc ccgctggctg gcgaaaacca tcaccctctc
     1261 gccacatact cagcgtaact atccggagtt gcaaaaacgg ctgaatggct ggctttcggc
     1321 ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggcg gtaatgttgc tgttgagcgc
     1381 atggggattg ttcgatttct ggaactggct gcaaaacggc gcggggcaga aaaccgtaga
     1441 tatcctgatc cgtatcgcac ttattctttt tttctcggcg gttggctgga cggtgctcgc
     1501 cagtttgatc gaaaaccggc tggcttcgga tattcatggc cgcccgctac ccagcgcccg
     1561 tacgcgtacc ctgctgacgc tgtttcgtaa cgcgctggcg gtgattatca gtaccatcac
     1621 catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg ccattgctgg caggtgccgg
     1681 ggcattaggt ctggctatct cgtttggttc gcaaacgctg gtgaaagata tcattaccgg
     1741 ggtgtttatt cagtttgaaa acggcatgaa cactggagat ttggtgacta tcgggccgtt
     1801 gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc gtgcgccagg ataccggggc
     1861 gtatcacatc attccgtggt cttcgataac cacctttgct aacttcgtcc gcggtattgg
     1921 ttcggtagtg gcaaactacg atgttgatcg ccatgaagat gctgataaag ccaatcaggc
     1981 actgaaagat gcggtagcgg aattgatgga aaacgaagaa attcgcgggc tgattattgg
     2041 tgaaccgaat tttgccggga ttgtcggctt aagcaatacc gcgtttacac tgcgtgtttc
     2101 gttcaccacg ctgccactca aacagtggac ggtacgcttt gccctcgaca gccaggtgaa
     2161 aaaacatttc gacctggtgg gcgttcgcgc gccagtgcag acttattagg tgctgcctac
     2221 tccgggcgcg accccggctg aaccgttacc gccgggggag ccaacgcttt aacgctggcg
     2281 attgacaaaa cggcggcgct gctcgtcgtt cataaaggtc caggcaataa agcgactctg
     2341 cttttgcccc tgggccatct ctttttttac caccttcacc gcgcccacgt cggtcagagc
     2401 acgatacaac ggcggtaagt tttcaccacg agataccagc gaggtaaacc acatcacctg
     2461 cttcgcgaag cctttgctct cttcaatcat ctttttgata aaggcgactt caccaccttc
     2521 acaccacaac tcctgttgct gaccaccaaa gttcagtgca tcgtctttgt tcagccccag
     2581 gttacggcgt ttacgctcac tacctgcccg cgctgcagcg gcggaatcgt ggaatggcgg
     2641 gttacacaag gtcgcgtcgt attgctcgtt tttatggatg atgccgttaa aaattgcccc
     2701 actctctttt tgccgacgca gacgaatggc gcggttaagc cccggattag cactgataat
     2761 cgcctgcgca ctgcttaacg cctggctgct ggtttcacta ccggtaaaac gccagccata
     2821 ttcatgtacg ccaatcagcg gataaataca gttcgcacca acgccgatat ccagaatgct
     2881 ggcattcgct ggaattgttc cgcttgcttc agccagtaaa tcggcaaggt gatgaatata
     2941 atccgcccgg ccgggtactg gtgggcagag aaaaccgtcg gggatatccc agttcgctac
     3001 ggcgtaaaaa tgggccagca acgccttatt gagcgccttc accgccagcg gattggcaaa
     3061 gtctacgctt tgctccccgg cgggtgtaag cgtgaggaat tgcctgagtt caggattgac
     3121 ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg ttgcgcggat gcaaccccgg
     3181 tttctgggcg gacatagcat tctcctttaa gcagcggcgt aagatacccg cataacgcga
     3241 cagggtaaat atctggctgc gtttatcatt ccccaaaacc ttaacatggc tatggtttat
     3301 ggcaattcat cataaaaatc agcattatgc atgtcattga tttgtcttat tttgtaaaaa
     3361 tatatcgttc aaaaaataag cacatctctg tcaatttttt gcgtcatttg catcgcgcag
     3421 gcgacctaca cttatcaggc actacccgtt gtatttatga ggatgtcatt atgaaaaagt
     3481 gcctcacact actgattgcc accgtcctga gcagaatctc tctcacggct tatgccgcgc
     3541 aaccgatgag taacctggac agcggtcagc tacggcccgc ggtaccgttt cggcaactgg
     3601 cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg aacaaggcgc
     3661 gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttcggta ctgcaaccat
     3721 ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt cgcctgtttt
     3781 gtaatcaaaa aacatcaaat atgaactcaa tgtaaataaa tgtatttctt tttcgcgcaa
     3841 tgggtgatag aaaatcgcgc caagtgataa tgcttaccaa aattattatc acttgcatga
     3901 acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc gtgacagtga
     3961 aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca atttccctgc
     4021 cagacaattt cattcgctca cgttctttgc cggtctttgt attggcatca cgcctgtggc
     4081 tcaggcactc gccgccgaag ggcaaactaa cgcggatgac acgctggttg tcgaagcatc
     4141 aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc gtccggtagc
     4201 ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc agggcgcaac
     4261 caaccttacc gacgcgctca aaaacgtccc cggc
//
LOCUS       DADLTD010000259         4266 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLTD010000259 DADLTD010000000
VERSION     DADLTD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.263"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..212)
                     /locus_tag="JN701_003802"
     CDS             complement(<1..212)
                     /locus_tag="JN701_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7366086.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPV"
     gene            522..2294
                     /gene="aspS"
                     /locus_tag="JN701_003803"
     CDS             522..2294
                     /gene="aspS"
                     /locus_tag="JN701_003803"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY7366087.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2412..2864
                     /gene="nudB"
                     /locus_tag="JN701_003804"
     CDS             2412..2864
                     /gene="nudB"
                     /locus_tag="JN701_003804"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY7366088.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2893..3633
                     /locus_tag="JN701_003805"
     CDS             2893..3633
                     /locus_tag="JN701_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7366089.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3668..4189
                     /gene="ruvC"
                     /locus_tag="JN701_003806"
     CDS             3668..4189
                     /gene="ruvC"
                     /locus_tag="JN701_003806"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY7366090.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
BASE COUNT         1052 a         1060 c         1199 g          955 t
ORIGIN      
        1 tcaaccggct gttttaatgc ttcggcgtaa tcggcagacc agccaactcg caccagaaac
       61 acgggctgac cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca
      121 tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc
      181 aacgcagtgg ttttagcatt aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc
      241 ctcacacctt aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt
      301 tcgggcaaca cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg
      361 gtaacgaggc gttccagcgc gggctaggct cttacggtgc ggcagaattt ccagtataat
      421 agccgccttt tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc
      481 agcagggggc atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg
      541 gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc
      601 gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg
      661 tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt
      721 tctgcattca ggtcacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata
      781 tggcgaccgg cgaaatcgaa gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg
      841 ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc
      901 tcgacctgcg tcgtccggaa atggctcagc gcctgaaaac ccgcgctaaa atcaccagcc
      961 tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg atgctgacca
     1021 aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat
     1081 tctacgcact gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggttttg
     1141 accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc
     1201 ctgagtttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag
     1261 tgatggaagc gctggtgcgt catttgtggc tggaagtgaa gggcgtggat ctgggcgatt
     1321 tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa ccggatctgc
     1381 gtaacccgat ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat
     1441 ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg
     1501 catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga
     1561 aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctggaaggt atcaacagcc
     1621 cggtagcgaa gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc
     1681 aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgcgatgg
     1741 gcgcactgcg cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac
     1801 cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa
     1861 tgcaccatcc gttcacctca ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg
     1921 aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt
     1981 cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg
     2041 aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc
     2101 cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgact ggcaccgaca
     2161 atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag
     2221 caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg
     2281 cagagaataa ctgatatgac tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa
     2341 ttgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa ggcagtcaac
     2401 gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct
     2461 atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc gatttctggc
     2521 agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag
     2581 taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gacagtcagc
     2641 gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc
     2701 gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg
     2761 aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga
     2821 gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct
     2881 ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg
     2941 caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct
     3001 aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca
     3061 ctgtctaaca acatgacccg tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt
     3121 gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca
     3181 atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcatgca
     3241 ttcagcaaat gtggcggtaa cctcggtaca gatggttccg tagcctatct gttcagcaaa
     3301 aaaggcgtga tctccttcga gaaaggcgat gaagacacca tcatggaagc agctctggaa
     3361 gcaggtgctg aagacgttgt gacctatgat gacggcgcga ttgatgtcta cactgcatgg
     3421 gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg
     3481 gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc accgaaactg
     3541 atgcgtctga tcgatatgct ggaagattgc gacgacgtgc aggaagttta ccataacggt
     3601 gaaatctctg atgagattgc agcgactctc tgatgaggcc tgctaaacag caaaacggag
     3661 acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg gctatggcgt
     3721 catccgccag gtaggcaggc aactgtccta cctgggtagc ggatgcatac gcaccaaggt
     3781 ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca
     3841 gttccagcca gattatttcg ccattgaaca agtctttatg gccaaaaatg ccgactcggc
     3901 tctgaaactg gggcaggcgc gcggcgtggc gattgtggcg gcggtgaatc aggaattgcc
     3961 ggtatttgaa tatgcggcac gtcaggtaaa gcaaacggtg gtaggtattg gtagtgccga
     4021 aaaaagtcag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc
     4081 ggatgccgcc gatgcgttag ctatcgcgat tacccattgt cacgtcagcc agaatgcaat
     4141 gcagatgagc gaatcgcggc tgaacctggc gagagggcga ctgcgttaag ttataccgcc
     4201 tcggtcagtt caggctgagg cgtttcaact ccctccgcgt gaatcaacac caaacccgga
     4261 tgatgc
//
LOCUS       DADLTD010000260         4246 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLTD010000260 DADLTD010000000
VERSION     DADLTD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.264"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            154..657
                     /gene="yeaK"
                     /locus_tag="JN701_003807"
     CDS             154..657
                     /gene="yeaK"
                     /locus_tag="JN701_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY7366091.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(658..762)
                     /locus_tag="JN701_003808"
     CDS             complement(658..762)
                     /locus_tag="JN701_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366092.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            931..1378
                     /locus_tag="JN701_003809"
                     /pseudo
     CDS             931..1378
                     /locus_tag="JN701_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
     gene            complement(1335..2156)
                     /locus_tag="JN701_003810"
     CDS             complement(1335..2156)
                     /locus_tag="JN701_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7366093.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2253..3449
                     /gene="nimT"
                     /locus_tag="JN701_003811"
     CDS             2253..3449
                     /gene="nimT"
                     /locus_tag="JN701_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAY7366094.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3489..3836
                     /locus_tag="JN701_003812"
     CDS             3489..3836
                     /locus_tag="JN701_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY7366095.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKNQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3858..4112)
                     /gene="yoaF"
                     /locus_tag="JN701_003813"
     CDS             complement(3858..4112)
                     /gene="yoaF"
                     /locus_tag="JN701_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY7366096.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
BASE COUNT          978 a         1077 c         1052 g         1139 t
ORIGIN      
        1 gaacaaatgg tagtgtccgc tattgccagt acacctcagt tacatgatgc gtataaagct
       61 tccgatgagc gtttatatgt caataagcag aacaaaaaca gccgttcatg ataaccttct
      121 gtggttgttt gcttgtaatc tcaggagcgt gaaatgactg aaatggctaa aggaagcgtg
      181 acgcatcagc ggttaatcgc tttattatca caagaaggtg ctaacttccg cgtcgttacc
      241 catgaagcgg taggtaaatg tgaagcggtg tcagaaattc gtggcaccgc tttaggccag
      301 ggagctaagg cactggtatg taaagtcaaa ggcaatggcg taaatcaaca tgttctggcg
      361 atcctcgctg ccgatcaaca ggccgatctg agccaacttg ccagccatat aggcggatta
      421 cgcgcttcgt tagccagccc tgctgaagtc gatgaactca caggctgtgt cttcggcgcg
      481 atcccccctt tcagcttcca tccaaaactc aaactggttg ccgaccctct actcttcgaa
      541 cgatttgatg aaatcgcctt caatgcaggg atgctggata aatccgttat tttgaaaacc
      601 acggattatt tgcgcattgc gcaaccagaa ctggtcaatt tccgccgcac tgcgtaacta
      661 gcctgtccgt tcaataagca gaacggacaa aaccagcaca acagcaatgg caaaaaacga
      721 tgacgtgata atcagtgtct cgacgaacat ttgatcgttc atagcatgcc cccgggagat
      781 agctgttttc acgttattgt tagcgtgcac aaatggcagt ttgatgacag ttcgcctgat
      841 ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt aaagtaagca
      901 ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct gatcctgctc
      961 gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc aattctggtg
     1021 ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga aaaacagggg
     1081 ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcgc cagcggcgac
     1141 gctaccgccc tcaaccttga ttcactcgtt tctgaactgg aaatcgctgg tggcaattgc
     1201 agtaggcgtg attgtctcct ggctgggtgg gcgcggcgtg accttaatgg gcagccagcc
     1261 gcaactggtc gccgggctgc tggtaggcac tgttttaggc gtggcgctgt ttcgcggcgt
     1321 accggtcggc ccgcttattg ccgcgggtct ggtttcgctg attgtgggga aacagtagtt
     1381 aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt tcttaaacat agtaataaag
     1441 gccgttgttg aatcataccc cagcgtatga gcaactttct gtacagtatc gcctttcact
     1501 aatccctgaa gtgccataat cagctgtaac tgctgacgcc actggcgaaa actcaacccc
     1561 gtctctttta cgatcagtcg cgcaagattg cgttcactca tcgcaaaaaa gccagcccat
     1621 tgccctaatg acccccactc gacgggccct ttcgccatca tctccaccat cgtgcggatt
     1681 ttaggatgag aagaaacggg caggtgcagc tgttgttgtg gctgctgagg gagttcatca
     1741 aacaggacct gtattaatct gcgggtcatc ggctcagcac gctgagtcgt cgtcctgttt
     1801 gccagggtta aaatcaattc ccggcataag ggcgatattt tcaatgtgca acatgttgtc
     1861 ggcattgtca ctgcgctggg ttcgataaac agaaaacaga gttcagcgtt agctgtcact
     1921 tgattgctat gttccacccc gcccggtatc caaaccgcat attgcggcgg caccatccac
     1981 aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg ctagtatcag ctgtcctttg
     2041 cggtgctgat gtacaggact ggttaactca tccgttccgg cgtgaataca aaatgcgacc
     2101 gcggcttcat ggtgtcggtc gggttcatag ccattgagat tcaacctgtg catcattttg
     2161 tccgaattta gcgataattt gtcattttag cttgattcaa cataacaata aaaacggtaa
     2221 ggtacagcct cgtttgtaac aatgagaagc atatgacctg ttcaacttca ttaagcggca
     2281 aaaacaggat tgtccttatc gctggcattc tgatgattgc cacaacatta cgcgtcacct
     2341 ttaccggcgc agcaccgtta ctggatacga ttcgttccgc ttactcgctg acgacagcgc
     2401 aaaccggctt attgaccacc ctgccattat tggcctttgc gctaatctca cctttggccg
     2461 ccccggtagc gcgacgtttt ggtatggaac gtagcctgtt tgccgcgtta cttttgatct
     2521 gtgctggtat cgcaattcgc tctctccctt cgccttactt attatttggc ggtacagcgg
     2581 tcattggcgg tgggattgca ttaggcaatg tcttactgcc aggattaatt aaacgcgatt
     2641 tccctcattc cgtcgccaga cttaccggcg catattccct gacaatggga gctgcggcgg
     2701 cacttggatc ggctatggta gtgccgctgg ctttgaacgg ttttggctgg caaggcgcgt
     2761 tgctcatgct gatgtgtttt cctctgctgg ctcttttttt atggctgcca cagtggcgaa
     2821 gccaacaaca tgcaaatttg agtacctcgc gcgccttaca tactcggggt atctggcgtt
     2881 cgccgcttgc ctggcaggtc acattgtttc ttgggagcaa ctcactggtc tattacgtga
     2941 ttattggctg gcttccggcg atcctcatca gtcacggcta tagcgaagca caggcgggtt
     3001 cactgcatgg tttgctgcaa ctagccacag cagctcccgg tttgctgatc cctcttttct
     3061 tacatcatgt gaaagaccag cgtggtattg cagcgttcgt tgccttgatg tgcgcagtgg
     3121 gcgctgttgg gctctgcttt atgccagcgc acgcgatcac ctggactctg cttttcggtt
     3181 ttggttccgg cgcaacaatg atactggggt tgacgttcat tggtcttcgg gcaagttctg
     3241 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     3301 gtgggccgcc gctaatgggt aaaatacacg atgctaacgg taactggtcc gtaccactga
     3361 tgggtgttgc aatactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     3421 aagaaattcg ctcatatccg gtgctatagt gacgtgacaa atcatgcgtg aaagggagaa
     3481 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     3541 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     3601 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     3661 tcgtcgattt tacaaccttt cgcgagcaat atcttgcaga actggcgcaa cacgagcaag
     3721 aaggaaagcg gctggcggac atcgccaaaa accagccgct gaccctgctc tacgcagcaa
     3781 aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt
     3841 agtacagcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     3901 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtcgac
     3961 actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     4021 atctgcggcg gtttaggtgc ttctggctga gaaggggttg aacaaccagc caggaccagc
     4081 aagcaaggca gaacaaaact gataattttc atttattgat ctcacatatt tatccagact
     4141 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     4201 tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctg
//
LOCUS       DADLTD010000261         4194 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLTD010000261 DADLTD010000000
VERSION     DADLTD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.265"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1170)
                     /locus_tag="JN701_003814"
     CDS             complement(<1..1170)
                     /locus_tag="JN701_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY7366097.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSF"
     gene            1661..1771
                     /locus_tag="JN701_003815"
     CDS             1661..1771
                     /locus_tag="JN701_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY7366098.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            2051..3046
                     /gene="pgl"
                     /locus_tag="JN701_003816"
     CDS             2051..3046
                     /gene="pgl"
                     /locus_tag="JN701_003816"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY7366099.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(3087..4039)
                     /locus_tag="JN701_003817"
                     /pseudo
     CDS             complement(3087..4039)
                     /locus_tag="JN701_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1122 a          858 c          995 g         1219 t
ORIGIN      
        1 gaaagactgc tgtcgaagct ccgcagaggc actgagtttt tccagccatg cagcgacctg
       61 ttcacggaat ccggaggtat tgcgtgcaga gacggtatcg gaaaggcggt caaggaacgc
      121 ggagaatgcc cgggaattct cctcggtgga aaaagaagtc cattgtgtag aaacctcccg
      181 acgatctgct tcaggaaacc aggctgccac agtttcggga agagggggga tctgaaagtt
      241 ctgcccccct tcagtgatga ctctgatatg gcgcgaatgt aaattttgcg gcaacatctg
      301 gcgtaaaaac agtctgtttt ggtctgacag ggaaatacga aactgaggcc cgttaaaatt
      361 tggaaccgaa gtttgctgta ttaagaatag cagagtttga tctgataagg ggttatttgc
      421 aatctcaata gttatatgag gatctaaacg aaaaagattt gtcgggattg tagttatttg
      481 gttgttttca aaataaaaaa atcttctcat gataatattt tcttggatgt ttggtaagtt
      541 gcttaatcta ttgctatatg caaaaagaag ttgcagtctc ctaggtaaag ggggcagttc
      601 agtaagatgg ttgtgcgcaa ccttaagtat cttaagatta gaaggcagag atggaagagt
      661 ttgcagttcg ttgttcctaa cctcaagttt ttttaaattc aagggtaatg aaggcagtgt
      721 ggataaccta ttatactctg cgcttagtga tttgatggtg tctggtaata acggcagatt
      781 ctctaattga ttttcggata catccaaagt tattagataa tgtggtaatg cagatatatg
      841 tgttaagaaa ttatctcttg caataagttc tttaatattt tcaggtaaat cagtaaggat
      901 ggttaattta ttacagctca catcaagctt tttaatgcaa gcagggagta caggtaacga
      961 ggaaagcgcc aaggctcgca ggtccagttt atgcccctta gaggtaagac atgcacgaag
     1021 tcttcttacc gcctcagtac gctgttcttc cggtaacccc tgtttctccc aatcatccca
     1081 ttcggacaaa taatggttgg gggatgtaat tgtgctgata tgtggaaata aacaggatga
     1141 aatatttctg agcatattaa tttctcgcat atggatatgg aattagattg tgaataaatt
     1201 gtatttaatg tatctctcct aatgaggagg gtgtttcaga atattcgcca taagggaatg
     1261 agtgttatat catgctttct atcctggtaa gtatatttgc ttttttcgta tatttggttc
     1321 atcttttttt gatgttttac atattcacaa tgcagaggat ttataccaga agacattagt
     1381 cttgagtaag atactacaac atctccaact attttagcca gcctgactac ttgacttggt
     1441 aatatttgtt gctaataatc atgtataaaa taagcttata tacatagtaa cttcagcaaa
     1501 ggatttagta atgatccaaa tcatatatca atgtactgtt tttttcttta aaaaaacagt
     1561 acattctatt ttataatgac tgtgtattcc catggaagga gatcacccaa ttctcccccc
     1621 cgacttttga cgcagtgtct tgttaacagg gggatttacc atgcttattg gctatgtacg
     1681 cgtatcaaca aatgaccaga acaccgatct acgacgtaat gcgttgaact gtgcaggata
     1741 tgagctgatt gttgagcgaa tgggagcttg attggaagtt gttagggtat ggtgtttacg
     1801 gtcgttgata tggcgaacaa acgcggggag cgtcacgctc cctaaatggt cgcgaaaata
     1861 tctgtggcat tgtcaaattt gtgacggtga ctaatatttt aatgatatgc cattcgaaat
     1921 ggtgtgctaa cgtattgatt taattgtgac gcgatgttgt attttatcgc gttttgttgt
     1981 ctgtttttca ttcgcttgat gttgctattt acatgaaaaa taaagaattt attgtttttg
     2041 aggtgtgttt atgaagcaaa cagtttatat cgccagccct gagagccagc aaattcacgt
     2101 ctggaacctg aatcatgaag gcgcactgac gctgacacag gttgtcgatg tgccggggca
     2161 ggtgcagccg atggtggtca gcccggacaa acgttatctc tatgttggtg ttcgccctga
     2221 gtttcgcgtc ctggcgtatc gtattgcccc ggacgatggc gcactgacct ttgccgcaga
     2281 gtctgcgctg ccgggtagtc cgacgcatat ttccaccgat caccaggggc agtttgtctt
     2341 tgtaggttct tacaatgcgg gtaacgtgag cgtaacgcgt ctggaagatg gcctgccagt
     2401 gggcgtcgtc gatgtggtcg aggggctgga cggttgccat tccgccaata tctcaccgga
     2461 caaccgtacg ctgtgggttc cggcattaaa gcaggatcgc atttgcctgt ttacggtcag
     2521 cgatgatggt catctggtgg cgcaggaccc tgcggaagtg actaccattg aaggggccgg
     2581 cccgcgtcat atggtattcc atccaaacga acaatatgcg tattgcgtca atgagttaaa
     2641 cagctcagtg gatgtctggg aactgaaaga tccgcacggt aatatcgaat gtgtccagac
     2701 gctggatatg atgccggaaa acttctccga cacccgttgg gcggctgata ttcatatcac
     2761 cccggatggt cgccatttat acgcctgcga ccgtaccgcc agcctgatta ccgttttcag
     2821 cgtttcggaa gatggcagtg tgttgagtaa agaaggcttc cagccaacgg aaacccagcc
     2881 gcgcggcttc aatgttgatc acagcggcaa gtatctgatt gccgccgggc aaaaatctca
     2941 cctcatctcg gtatacgaaa ttgttggcga gcagggacta ctgcatgaaa aaggccgtta
     3001 tgcggtcggg cagggaccaa tgtgggtggt ggttaacgca cactaaccgc tgatttaacc
     3061 ggcgcagtct ctcctgcgcc ggtgtattaa cctatctcct gtaacgcatg tctctggcgt
     3121 tcgacgatat tggtccacaa attgtcttta tcgtcagtcc acaaattaat cagcaaggca
     3181 aaaaagcgtt cagctgccgg agaaagcacg gcatctttgc ggcgaataat ccccaatgtc
     3241 cgacgtataa cgggttccac cagtgggata ccaataatcg aagaataggg cgcatggggc
     3301 attgccagac caggaagcgc cgaaatcccc agtcctgcct ccactagtcc taatgacgtc
     3361 gaaagatgac gcacttcgta aaaccagtcc agcttccagg gcttgtcggc cagctgttgt
     3421 tctatcaaca gtcggttgcc gctggaggag cgtacgccaa tcattttgta acccactaac
     3481 tcctgccatt ctacaagctg ctttttggcc aatggatggt cacgccgaca ggcagcacga
     3541 acggctcgtt gaccagtggg gtaaaatcaa tggatgaatt tgtgacgttg ttcatgttta
     3601 tgccaaagtc tgattcgtta cacagcaccg attccatgca attattcgtg ccttgttcca
     3661 gaatccgcac tttaatattg ggatacagct cattaaattt accgattgcc agcggtaaaa
     3721 aataaaatac tgcggtcgga atacacgcta acgtcaccat accacgatga taagcattca
     3781 tatcacgaat attaaaaagc gtttcatcga atttctttat taattcccgc gcctccggga
     3841 gcaacctttt tcctgctttt gttaacgtaa ccttgcgagt tgtacgctca aatagttgaa
     3901 tatgtaaatc ttcctccatc ttttttattc ttcgcgttaa tgcaggttgc gtaatattga
     3961 gtaatttagc ggcattgtta aacgaactgg actctgccag tatgacaaat gccttcatac
     4021 ttgataattc atgcttcatt attactccgg aaaatggaag cgacgatttt gggtggctgg
     4081 ccgttaaaaa ttttaactgc atttagccaa cttaaattaa tgaaaaaatg ttattaatcg
     4141 ttgagctaaa gtcattagag atgctttgcc cttaatgtaa ccatatcgca ataa
//
LOCUS       DADLTD010000262         4183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLTD010000262 DADLTD010000000
VERSION     DADLTD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.266"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN701_003818"
     CDS             complement(<1..209)
                     /locus_tag="JN701_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7366100.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JN701_003819"
     tRNA            complement(324..399)
                     /locus_tag="JN701_003819"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JN701_003820"
     tRNA            complement(406..480)
                     /locus_tag="JN701_003820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JN701_003821"
     tRNA            complement(597..681)
                     /locus_tag="JN701_003821"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JN701_003822"
     tRNA            complement(690..765)
                     /locus_tag="JN701_003822"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JN701_003823"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JN701_003823"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY7366101.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN701_003824"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN701_003824"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY7366102.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN701_003825"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN701_003825"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY7366103.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1078 a         1041 c          933 g         1131 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DADLTD010000263         4174 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLTD010000263 DADLTD010000000
VERSION     DADLTD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.267"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..328
                     /locus_tag="JN701_003826"
     CDS             <1..328
                     /locus_tag="JN701_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7366104.1"
                     /translation="RLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRI
                     LGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPW
                     IWRHRK"
     gene            374..583
                     /gene="hha"
                     /locus_tag="JN701_003827"
     CDS             374..583
                     /gene="hha"
                     /locus_tag="JN701_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7366105.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            622..1212
                     /locus_tag="JN701_003828"
     CDS             622..1212
                     /locus_tag="JN701_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7366106.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1452..1712
                     /locus_tag="JN701_003829"
     CDS             1452..1712
                     /locus_tag="JN701_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7366107.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1936..2010
                     /locus_tag="JN701_003830"
     CDS             1936..2010
                     /locus_tag="JN701_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7366108.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            2003..2860
                     /gene="repA"
                     /locus_tag="JN701_003831"
     CDS             2003..2860
                     /gene="repA"
                     /locus_tag="JN701_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7366109.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(3913..>4174)
                     /locus_tag="JN701_003832"
     CDS             complement(3913..>4174)
                     /locus_tag="JN701_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7366110.1"
                     /translation="QAAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCG
                     LRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         1124 a         1049 c         1034 g          967 t
ORIGIN      
        1 caggctggta aatattgatg caccggaaaa gaaacaggat tatggccggt ggtcaacaga
       61 catgatgaaa tcactggttg caggaaaaac ggttaccgtc acatattttc agcgagatcg
      121 ttatggtcgt attctcgggc aggtttatgc accagacgga atgaatatta atcagtttat
      181 ggtccgggcc ggggctgcct gggtatatga gcagtacaac acagatcccg tgctacctgt
      241 ccttcagaac gaagccagac aacaaaaacg tgggctctgg tcagatgctg atccggttcc
      301 cccctggata tggaggcacc gtaaataaag cgatggcaat cctatcattc atctgttctc
      361 agcgggaata aaaatggcaa aaacaaaaca ggaatggctg tatcagttac ggcgatgttc
      421 atcagtcaat acgctggaaa aaattattca taaaaacaga gacagtttat caaacagtga
      481 acgtgaatca tttaattcag cagcagacca ccggctagcg gaattaatca ccggaaagtt
      541 atatgacagg ataccaaaag agatatggaa atatgtaagg taataattca gcaataatta
      601 attgccagga gcagataaat aatgaagttg attattttta ttctcattgt tctgattatt
      661 gcagcgctac ttatccggat tatcctcaga agtgtaaatc agcattctcc tttgctgatg
      721 caattgcatg cggcaggtat acggaccggt gatgcagagc gaatactgtc cagcggtgaa
      781 tactggcaac gtcagacgac gctgctgaca gaaagggaag tcagttttat gaaaggactg
      841 ttcagaattg tggatatgac gcggtggtat ctgtgtccgc aggtacgggt cgcggatatc
      901 gtccagctga acgggaatat ccggccacga tcacgccagt ggtggcagtt attcaggatg
      961 gtgtctcagt ggcatgttga tgtggttatc gttgagctgc gttcgttcag tattgttgca
     1021 gcagtagagc tggatgatgc cagccattta cgaccggaac gcagacgccg ggatattctt
     1081 ctggaagagg ttctgaggca ggctggtatt ccgttgctca gaagccacga tgccagaaaa
     1141 ctgctgcaga tgaccggaga atggctgaat acaacagggg ctgatcagca gtcaccggaa
     1201 catcgtagct gacgccttcg cgttgctcag ttgtccaacc ccggaaacgg gaaaaagcaa
     1261 gttttcctcg ctcccggcgt ttccataact gaaaaccata ctatttcaca gtttaaatca
     1321 cattaaacga cagtaatccc cgttgatttg tgcgccaaca cagatcttcg ccacaattct
     1381 caagtcgctg atttcaaaaa actgtagtat cctctgcgaa acgatccctg tttgagtatt
     1441 gaggaggcga gatgtcgcag acagaaaatg cagtgacttc ctcattgagt caaaagcggt
     1501 ttgtgcgcag aggtaagcct atgactgact ctgagaaaca aatggccgct gttgcaagaa
     1561 aacgtcttac acacaaagag ataaaagttt ttgtcaaaaa tcctctgaaa gatctcatgg
     1621 ttgagtactg cgagagagag gggataacac aggctcagtt cgttgagaaa atcatcaaag
     1681 atgaactgca gagactggat atactaaagt aaagacttta ctttgtggcg tagcatgcta
     1741 gattactgat cgtttaagga attttatggc tggccacgcc ataaggtggc agggaactgg
     1801 ttctgatgta gatgtacagg agccagaaaa gcaaaaaccc cgataatctt ctttaacttt
     1861 ggcgagtcag aaagattacc ggggcccact taaaccgtat agccaacaat caagctatgc
     1921 ggggagtata gttatatgcc cggaaaagtt caagacttct ttctgtgctc gctccttctg
     1981 cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acttattacc gccaggtaaa
     2041 gaacccgaat ccggtgttca caccccgtga aggtgcccgg acgcttccgt tctgcgggaa
     2101 actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc atgtggcaca
     2161 tgcccgttcg cttggtctgc gtcggcgcat gccaccggta ctacgtcgcc gggccattga
     2221 tgcgctgctg caggggctgt gttttcacta tgacccactg gccaaccgcg tccagtgttc
     2281 catcaccacg ctggccattg agtgcggact ggcgacggag tctgctgccg gaaaactctc
     2341 catcacccgg gccacccggg ccctgacgtt ccttgcagag ctgggactga ttacctacca
     2401 gacggaatat gacccgctta tcgggtgcta cattccgacc gatatcacgt tcacatctgc
     2461 actgtttgct gccctcgatg tatcagagga ggcagtggcc gccgcgcgcc gcagtcgtgt
     2521 ggaatgggaa aacagacagc gcaaaaagca ggggctggat accctgggta tggatgaact
     2581 gatggcgaaa gcctggcgtt ttgttcgtga gcgtttccgc agttaccaga cagagcttaa
     2641 gtcccgtgga atgaagcgtg cccgtgcgcg tcgtgatgcg gacaggcagc gtcaggatat
     2701 tgtcaccctg gtgaaacggc aactgacgcg tgaaatctcg gaagggcgct tcactgccag
     2761 tcgtgaggcg gtaaaacgtg aagtggagcg tcgtgtgaag gagcgcatga ttctgtcacg
     2821 taaccgtaat tacagccggc tggccacagc ttccccctga aagtgacctc ctcagaataa
     2881 tccggcacgc gccggaggca ttcgcacgcc tgaagcccgc cagtgcacaa aaaatcagcg
     2941 ctacatacaa aaaacaacct catcatccac cttctggtgc atccggtccc ccctgttttt
     3001 gatacaaaac acgcctcaca gacggggaat tttgcttatc cacattaaac tgcaagggat
     3061 ttcaccataa ggttacaacc gttcacgtca taaagcgcca gccgtcagcc ctacagggtg
     3121 caatgtatct tttaaacacc tgtttatatc tcctttaaac tacttaatta cattcattta
     3181 aaaagaaaac ctgttcactg cctgtcctat ggacagacag atatgcacct cccaccacaa
     3241 gcggcgggcc ccgaccgaag ccggtttagt tacaacatgc aaacataaac ccctaaaaaa
     3301 cccaagtccg acgcagaact gaaaccacaa agcccctccc tcataactga aaagcggccc
     3361 cgccccggcc cgaagggccg gaacagagtc gcttttaatt atgaatgttg taactatatt
     3421 atcatcgctg tcagtcctct ggctggacgt atcgagtaca cgctcgtaag cggccctcac
     3481 ggccctcacg gcccgctaac gcggagatac gccccgactg cgggtaaacc cttgtcgggc
     3541 tccgaccgcg cacagaagct ctgtcatggc tgaaaacggg tatggcttgg cagctaccat
     3601 gttctgtcaa gaaaaatcgc ttgaactccg acgtacagcc ccctaagtac tgccagcaag
     3661 ctgtcagcac taagtccccg atacagcgcg gcgtagccgc gctaacgggg ttaaaagggc
     3721 ttacctatac ttcatcatcg ctgaaagccc gcatggcctc gcagggcaga gatgggatag
     3781 gtgaaaccta tcaatacgta ccggcttacg ccgggcttcg gcggtgttac tcctgtacca
     3841 tatgtaacta catcatgccg ccttacatgc gctacgcgca tatgaagaca aatagcccac
     3901 atcaaacggt ttttaggtct tcagtacgcc atagcaccag tgcacgagtt ttctcatcag
     3961 cgcaccaatg gcacacattt tggccttacc tctcaggcat aatcgctcat acatcgcttt
     4021 catcaatggg ttaaatctca gaccacacaa cgaagccaga tacagtttcg cccggatttc
     4081 cggcggacca atttttgaca gtttcggaca ccctctgacc gaggtgccgg aatatttttc
     4141 aacgggaacg acacctaaaa atgcagccgc ctgt
//
LOCUS       DADLTD010000264         4121 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLTD010000264 DADLTD010000000
VERSION     DADLTD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.268"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..149
                     /locus_tag="JN701_003833"
     CDS             <1..149
                     /locus_tag="JN701_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HAY7366111.1"
                     /translation="PDNEGHVSVRYAAANNLSVIGVTKEDKQRISEILQETWESADDW
                     FVSE"
     gene            439..693
                     /gene="bssS"
                     /locus_tag="JN701_003834"
     CDS             439..693
                     /gene="bssS"
                     /locus_tag="JN701_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY7366112.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            808..1926
                     /gene="solA"
                     /locus_tag="JN701_003835"
     CDS             808..1926
                     /gene="solA"
                     /locus_tag="JN701_003835"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY7366113.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1974..2087
                     /locus_tag="JN701_003836"
     CDS             1974..2087
                     /locus_tag="JN701_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY7366114.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2348..2914
                     /locus_tag="JN701_003837"
     CDS             2348..2914
                     /locus_tag="JN701_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY7366115.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2918..3493
                     /locus_tag="JN701_003838"
     CDS             2918..3493
                     /locus_tag="JN701_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY7366116.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3535..>4121)
                     /locus_tag="JN701_003839"
     CDS             complement(3535..>4121)
                     /locus_tag="JN701_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY7366117.1"
                     /translation="NHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYC
                     TGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGER
                     ISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPE
                     EQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT         1060 a          993 c          937 g         1131 t
ORIGIN      
        1 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
       61 ttggcgtaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
      121 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccgggtcgc
      181 cccggctttt ttattttact tacaaatttt gagtacaaaa tttctacact tcctcaaaat
      241 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
      301 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
      361 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
      421 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
      481 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
      541 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
      601 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
      661 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
      721 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
      781 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
      841 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
      901 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
      961 catgcttatg gtgaaggcga aaagtatgtc ccgttggtcc tccgcgcgca aacgctgtgg
     1021 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
     1081 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
     1141 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
     1201 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
     1261 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
     1321 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaaccgttga cggtgagtat
     1381 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
     1441 cctgtccagc ctgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
     1501 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
     1561 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
     1621 gcggatgaac gtgttccgtt tgcggaagtg gtcagcgatg gttcggaagc cttcccgttc
     1681 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
     1741 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
     1801 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
     1861 gattttgcgc aagacaaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
     1921 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
     1981 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
     2041 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
     2101 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgagattat
     2161 tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa cttatgaaga
     2221 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
     2281 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgtattgga
     2341 tgcacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca
     2401 ttggctaagc gcaattattg tctacggcat gtttacttta ggtctgtgga tggtcacgct
     2461 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
     2521 gttaatgatg gggctggtta ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc
     2581 gctgccgagt tattcgccca tgactcgtct tgccgcaaag gctggacatc tcgcattgta
     2641 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
     2701 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc
     2761 agactttgcc ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtccgttat
     2821 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
     2881 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
     2941 aaccttcgcg tccctgatgt tctctgccgg ttcagcggtt gccgccgatt acaaaattga
     3001 caaagaaggt cagcacgcct ttgttaattt ccgcatccag caccttggct atagctggtt
     3061 atacggcacc tttaaagatt tcgacggtac ttttaccttt gacgaaaaaa atccggctgc
     3121 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaatcacg ccgaacgcga
     3181 taaacatctt cgcagtgcag atttcctcaa taccgcaaaa tatccacagg caacattcac
     3241 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa
     3301 tggcgtaacc aaacccgtca cgctggaagc gaaattaatt ggtcagggcg acgacccatg
     3361 gggtggtaaa cgtgcaggct tcgaggccga aggcaaaatt aagctcaagg acttcaatat
     3421 caagacagat ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt
     3481 acagcagaag taagcgtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
     3541 tgttggatca ggaatgccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
     3601 attgccattt tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc
     3661 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcgcatactg gacactgaat
     3721 aaacagcagg tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca
     3781 ctggtggcaa tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac
     3841 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
     3901 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaagcact
     3961 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc
     4021 ctgcatcatc tcgactgctt ttggcagctg ctcacggaag gtatctgccg gaatttcgag
     4081 cgcgttttca aagtgcccca cttcatactc atagtggtta c
//
LOCUS       DADLTD010000265         4081 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLTD010000265 DADLTD010000000
VERSION     DADLTD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.269"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            33..932
                     /locus_tag="JN701_003840"
     CDS             33..932
                     /locus_tag="JN701_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7366118.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1269..2882
                     /locus_tag="JN701_003841"
     CDS             1269..2882
                     /locus_tag="JN701_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7366119.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2933..3964)
                     /gene="ynaI"
                     /locus_tag="JN701_003842"
     CDS             complement(2933..3964)
                     /gene="ynaI"
                     /locus_tag="JN701_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY7366120.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1145 a          896 c          961 g         1079 t
ORIGIN      
        1 taaatgatta atagattgca tggagatact gaatgaagcg tgaagaaatc gctgatctga
       61 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg tgcagcagcc cgcctgagca
      121 tggcgcagtc agctttaagc cagatagtgc gtcgtataga agaacgattg ggattgcggc
      181 ttctgacgcg aaccacgcgc agcgttgttc caactgaagc gggcgagcat cttttgtctg
      241 ttcttggccc tatgttgcat gacatagatt cagccctggc atccctgagc gagctgcaga
      301 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
      361 taccagcaat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag ctcaccattg
      421 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtccgt ctgggtgggg
      481 agatggataa agatatgatc gccattcgaa tcgggccaga tataccaatg gctattgttg
      541 gctcaccgga ttatttttct cgccgaagtg ttccaacgtc agtgtcacaa ttaatagatc
      601 atcaggcaat taatttgtat cttcccacat cgggtacagc aaatcgctgg agattaatac
      661 gcggtggacg tgaagttcgt gttcgcatgg aaggtcagct tttactgaat acgatagacc
      721 tgatcattga tgctgcaatt gatgggcatg gattggcgta tctaccttat gatcaggttg
      781 agcgggctat taaagaaaaa aaactgatac gtgttttgga taaattcaca ccagatttac
      841 ccggttatca cctgtactat ccacaccgtc gacatgctgg ctcggcattc tcattattta
      901 tagataggct gaagtataaa ggtgctgttt agcactactt gctgatacat taatttaatt
      961 tctctcttaa cgtattctca gttcctttca acgttttggt tattttttat tcttcgtaca
     1021 atggcgacag atgctgattt tgataccgaa aactggtttg aacgtgcgaa gcctgaacgt
     1081 agtattcggt gttctatgtg ctttgccgca ttttggcgtc gattatttac actaaatctg
     1141 atttaatata ttgatacata aaacatttta tgcttcctgt gtcacttttt tgatggaagt
     1201 tgtttgcatt tctttaaggc gaaacaaata attacgcatc aattttaatg tcggttagag
     1261 ggaaacttat gaagcactct gtttcagtca cgtgttgtgc gctgttggtc agcagcattt
     1321 ctctttcgta tgctgcagaa gttccgagcg gcacagtact ggcagagaag caggagctgg
     1381 tgcgccacat taaagatgag cctgcgtcgc tggatcccgc taaagccgtg ggcctgccag
     1441 agattcaggt cattcgcgat ctgtttgaag gactggtgaa tcagaacgag aaaggggaga
     1501 ttgtccccgg cgttgcgact cagtggaaaa gtaatgacaa ccgtatctgg acttttaccc
     1561 tgcgcgataa cgcaaaatgg gcggatggca caccggtaac ggcgcaagat tttgtctaca
     1621 gctggcagcg tctggtggac ccgaaaacgc tatcgccatt tgcatggttt gccgcgctgg
     1681 cgggaatcaa caacgcacag gcgattattg atggtaaagc tacgcctgac cagcttggcg
     1741 tcaccgcagt tgatgcccat accttgaaaa ttcagcttga taaaccgttg ccgtggtttg
     1801 tgaatttaac cgctaacttt gccttcttcc cggtgcaaaa agccaacgta gaaagcggta
     1861 aagagtggac gaaacccgga aatctgatcg gcaatggcgc ttatgttctt aaagaacgcg
     1921 tagtcaatga aaaactggtc gtggtaccga atacccatta ttgggataac gccaaaacgg
     1981 tactgcaaaa agtgaccttc ctgccaatta atcaggaatc cgcagccact aagcgttacc
     2041 tcgcggggga tattgatatc accgaatcct tcccgaaaaa tatgtatcag aagctgttga
     2101 aggatattcc ggggcaggtt tatacgccgc cgcagctcgg gacctattat tatgcgttta
     2161 acacgcaaaa agggccgacg gcagatcagc gcgttcgcct ggcattaagt atgacgatag
     2221 atcgccgcct gatgaccgaa aaagtgttgg gaacgggcga aaagccagcg tggcatttta
     2281 caccagatgt taccgccgga tttacgccgg aaccttcgcc gtttgaacaa atgagtcagg
     2341 aagaactgaa tgcgcaggca aaaactttgt tgagcgcagc tggttatggt ccgcaaaaac
     2401 cgctgaagct gacgcttttg tataacactt cagaaaacca tcaaaaaatt gcgattgctg
     2461 tagcatcgat gtggaaaaag aaccttggcg tagatgttaa attgcaaaat caggaatgga
     2521 aaacctatat cgatagccgt aacaccggca attttgatgt tatccgcgcc tcatgggtgg
     2581 gggattataa tgaaccctcc actttcctga cattattaac gtcaacgcat tcaggaaata
     2641 tttcacgctt taacaatccg gcatacgaca aagttctggc ccaggcatcg acggaaaata
     2701 ccgttaaagc gcgtaatgcc gattacaacg cggcagaaaa aatcctcatg gagcaagcac
     2761 cgattgcacc aatttatcaa tataccaatg gacgattaat caagccgtgg ctgaaaggtt
     2821 atcccattaa taacccggaa gatgtggcgt acagccggat tatgtatatt gtgaagcatt
     2881 gatgtgatgg gaactggcgt tacccttgtg gataacgcca gtgatgtctg atttagcgcc
     2941 cctgttccgg cggtgtaatg ttatccatat acagcgtctg gctggggaag gcaaagtccg
     3001 cgccgtgtga ctgcacaata tcgataatct tcaaataaac gtcttgttgt gcagcaagcc
     3061 attcagccca taccgtggtt ttggtaaagc aataaaccat aatattcaat gacgagtcgg
     3121 caaactggtt gaaataaacc agtaaggttt gtcgctggtc gatggccggg tgatttttca
     3181 gcatctcacg tacggcttcg acaataacgc ccacttttgc cgcatcctca taacgtaaac
     3241 caatggtcgt ggtaatgcgg cggttggtca ttcgtcctgg gttttctacg ctgatcgacg
     3301 aaaacagcga gttcggtacg tacaatggac gattatcaaa ggtcgtaatt ttggtaattc
     3361 gccagccaat ttccgctact gtaccttcga tatttctgtc cggtgaacgg atccagtcgc
     3421 caatactgaa aggacggtcg aaatagagca tgatcccgga aaagaaatta ctcagaatat
     3481 ctttaccggc cataccgaca gccagaccac caataccacc aaaggtcagc aagccagaaa
     3541 ggctcatgcc gaaatgttcg ccataaagca gaacaagcac cacaataatg gtgattttga
     3601 taatgcgcga cataatccgc gcactggtga tatcgcgacc ttttttaatc tgctgttttt
     3661 caaactgatt aatcagcaaa aatagcttaa tcgtcagaat aaccgcaatc agggacgtac
     3721 agataaaatc gataacgcct ggggtgataa atttgagttt atagttttct ataacataat
     3781 taataatgct accaacagca ctgataatta tggtgtagat taaaaattgc accgcatgga
     3841 ataaaaaacc ttttctttta cgatttccgc ggcgaaacca aaagctcatc agaatcaatg
     3901 ctgcgcagct accgaaaata atgaccagat taagcgcatt atttgtaaac agttcagcga
     3961 tcattgtttt atcaggctcc tccagataat tgtcgtcatg ccggaacccc ctggcggggc
     4021 tattttaccg cgacaattca ttcagatcat caatagtcag ggaaggaagt agcaacatta
     4081 g
//
LOCUS       DADLTD010000266         4056 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLTD010000266 DADLTD010000000
VERSION     DADLTD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.270"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(190..657)
                     /locus_tag="JN701_003843"
     CDS             complement(190..657)
                     /locus_tag="JN701_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366121.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
     gene            complement(1725..1916)
                     /locus_tag="JN701_003844"
     CDS             complement(1725..1916)
                     /locus_tag="JN701_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7366122.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            1960..2307
                     /locus_tag="JN701_003845"
     CDS             1960..2307
                     /locus_tag="JN701_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY7366123.1"
                     /translation="MIPVKRERMLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPP
                     SKTSGLPTLAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSEL
                     RDEVRKQGERDDS"
     gene            2297..2848
                     /locus_tag="JN701_003846"
     CDS             2297..2848
                     /locus_tag="JN701_003846"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY7366124.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELI
                     DATPFAKKYTSGVLSFAEQTLPPGERERVMESFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRRTLVTP
                     NNLPKAKQEAAEAITRGFTMPER"
     gene            2975..3535
                     /locus_tag="JN701_003847"
     CDS             2975..3535
                     /locus_tag="JN701_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7366125.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            3542..3775
                     /gene="mbeD"
                     /locus_tag="JN701_003848"
     CDS             3542..3775
                     /gene="mbeD"
                     /locus_tag="JN701_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7366126.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
BASE COUNT          971 a          932 c         1222 g          931 t
ORIGIN      
        1 aaaaaaaaac agtgtcatga ggtttacgca ggcaaaacca gcgttattca catggctgaa
       61 tagctatgca tacggttaaa atttatcagg tgcgatcgcg gcagcttttc gggtggtttg
      121 ttgctatttt tacctgtcag cagccgtgat cgcgctgaac gcatttcagc ggtgcgtaca
      181 attaagatat tacgttaaat tgtgagggaa tggtccccat atactgactt tatcataggg
      241 atagggttgg tagtgggtcg attgggtttc ggcattgttt ccgttaatgc caattgcatt
      301 tttgtatttg atgcaacaga aggcgtgaga ttctcctggg cctggaaagg ctaagtgttt
      361 agaattgatg gcaaaatatc ttccgtccga tttttgaagt agttgttgtt tggctgtctc
      421 ccaaagggtg ctgttacaaa cttcttgaat ttgtagtttt tgcagggctc ttattactgc
      481 gttgtcatgt tccaattctt taggggagtg tattattctc tcttttaata agctgtttac
      541 cacagggttt agttgttgtc taaagcacgc agataatgcc attgctacac aattttgttc
      601 ccgaggaagc atgtttggcg gggtatgccg tgtttgatgt gatatgtcta ttctcataga
      661 aggtctctgg cttatagcat caacaaatac ggctataaaa caaaattttc atagagtgat
      721 tcaagatctt cttgagatcc ttttttgcgg cgcgtaatct gctgcctgca aacaaaaaaa
      781 ccaccgctac caacggtggt ttgtttgccg gaatctagag ctaccaactc tttcttccga
      841 aggtaactgg cttggtggag cacagatacc aaatactgtc cttctagtgt agccgcagtt
      901 aggccaccac ttcaagaact cttaatatct caatccacct tgtccagtta ccaatggctg
      961 ctgccagtgg cgttaagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1081 cctacaccga actgagatac caacagcgtg agctatgaga aagcgccacg cttcccgaag
     1141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1201 agccatcagg gggaaacgcc tggtatcttt aagtcctgtc gggtttcgcc acctctgact
     1261 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccaccgg
     1321 cgcggccttt ttcgggctct tgttttttgc tcacatgttt cttttggctt tatcccctga
     1381 ttctgtggat aactgtgtta ccgtctttga gtgcgctgat accgctcgcc gcagccgaac
     1441 gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgccggaagc tacattttct
     1501 ccttacgcat ctgtgcggca tttcacaccc ggcatggtgc acttttcata caatccgcac
     1561 tgatgccgca taagtaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     1621 gcgccccgac acccgctaaa acctgctgac gcgccctgac gggcttgtct gctcccggca
     1681 tccgctcaca ggccagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcacca
     1741 tcatccccga agcgtgcgag gcagctgcgg taaagctcgt cagcgtggtc gtgaagcgct
     1801 tcgcagatgt cagcctggtc gtcggcgtcc agttcattca gtttttccag cagggtcagc
     1861 gtctggcttc tgatgaagcg ggccatgtta agggcggttt tttcctgttt agtcacagtt
     1921 ctgtttctcc atgtgtgggg agtctgtcgg aggtttttaa tgataccggt gaaacgcgaa
     1981 aggatgctca cgatacgggt cacggatgag gaacatgcgc ggctgctggc acgttgtgag
     2041 ggaaaacaac ttgcgtcatg gatgcgtaag gtctgcctgg gggcaccgcc atcgaaaacg
     2101 tcaggtcttc cgacactggc accgccgctg ttgcgccagt tcgcttcggt cggcaataac
     2161 ctgaatcaga tagcccggaa gataaacagc ggacagtggt cggggcatga ccgggttcat
     2221 gtggtggctg cactgatggc catcggacgt gaactgagtg agctgaggga tgaggtccgt
     2281 aaacagggag aacgggatga tagttaagtt tcatgccagg ggaaaaggcg gtggcagtgg
     2341 tccggttgat tacctgctgg gccgggagcg taaccgggaa ggggcgagag ttctgcgggg
     2401 tgctcccgaa gaggtgcggg aactcatcga tgccacgccg tttgcgaaaa aatacacctc
     2461 gggggttctg tcttttgcag agcagaccct gccaccggga gagcgtgaaa gggtgatgga
     2521 gagctttgag cgggtactga tgcccggtct tgaaaaaaat cagtacagca tcctgtgggt
     2581 ggagcaccag gacaaggggc gtcttgagct gaattttgtc atcccgaata tggagctggc
     2641 gagcggaaaa cggctccagc cctattacga ccgagcggac cgtccgcgaa tcaatgcctg
     2701 gcagacactg gtgaatcacc attacgggct gcacgacccg aacgcaccgg agaacaggcg
     2761 gacacttgtc accccgaata atctgccgaa agcgaaacag gaggccgcag aagcgattac
     2821 gaggggcttt accatgccgg agcgctaaaa acgcgtcagg atgtcactga ggcgcttaca
     2881 gcggcaggtt ttgaggttgt gaggacaacc aggagcagta tcagtatcgc cgacccggag
     2941 ggcgggcgta atctcaggct gaaaggggcg ctgtatgagc aatcttttac aaatggggac
     3001 agacttcgag aagaaactga aagagcgtgc cgtctctaca gagaacatac tgaacagcga
     3061 gttcaggaag ctcgaagcgt ctgtagacag ggaactgaac ttaaacgggc agaaaatcag
     3121 agacgctatc agcgcacaca cagcagcggt gaaagaacaa ctggagacgc tgaacagtgc
     3181 cgtgaacgcg cagttcagcg ccacagagaa cgaactggcc cggcggcagg aagagctgct
     3241 ctggaagctg gtcaaagggc ggatactgta cccgtcgctg acggcgcttt gcgtcacggg
     3301 cgggattttt ctggcgtcat gggggctgat tcagtggcag gaaagccgga ttgcggcgaa
     3361 catcctggct atcagggacc aggaagagac gctggcgaaa ctccgggaaa aaacatgggg
     3421 tgtgacgtat cacgagggca ggaacgggaa atttctggta ttgccgtccg gggtgaaggg
     3481 ggaaaacaac tggacggtgg agaagaagaa cgcagtaaga ctggtcaggg agtaagtgag
     3541 tatgacagag ctggaaatgc acttactgaa cgccttagag caattgcagc aggactatac
     3601 gcagcggctg aaagactggg agagcgcctt cacggatttg cagcatatgt tcgagctgac
     3661 acgacaggag aacgggcaac tgagggaaca gtgcgacgac ttgagtcagc aggtgcagag
     3721 attagtcgag caggtggacc acttgagtcg gttatttatc acgaacagca gataagggat
     3781 gaacaggaaa ggcaggcacg cctggaacgg gagcgtgcgc ggtcctggca tgaagagcag
     3841 gaacgggaag cacgggtatg gcgtggcccg tcaataggga tatgacggtg agttgttccg
     3901 ggaggttgcc agatggtacc ggaacgcgtt aaaaacgcat ccgggcgcga ttctggcgtg
     3961 tcgtttttgt tgctgtgttt tgcctctttg ccagatcgcg ctcattcctc agcctcatcc
     4021 cgcgatgtct gcgtatgtca tcaactgaaa agatgg
//
LOCUS       DADLTD010000267         4007 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLTD010000267 DADLTD010000000
VERSION     DADLTD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.271"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            95..745
                     /locus_tag="JN701_003849"
     CDS             95..745
                     /locus_tag="JN701_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HAY7366127.1"
                     /translation="MSLSPARQHRLRVQAEQAVREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLTEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            841..1305
                     /locus_tag="JN701_003850"
     CDS             841..1305
                     /locus_tag="JN701_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HAY7366128.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERFQDYDATASGAKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            1305..1508
                     /locus_tag="JN701_003851"
     CDS             1305..1508
                     /locus_tag="JN701_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366129.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            1512..1727
                     /locus_tag="JN701_003852"
     CDS             1512..1727
                     /locus_tag="JN701_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366130.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            1708..2220
                     /locus_tag="JN701_003853"
     CDS             1708..2220
                     /locus_tag="JN701_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY7366131.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALEKCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            2222..2599
                     /locus_tag="JN701_003854"
     CDS             2222..2599
                     /locus_tag="JN701_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366132.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALASLIKNGPLWQELRASLLKTITRKENVFISWVIRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITHNQMAQQVAA"
     gene            2596..3024
                     /locus_tag="JN701_003855"
     CDS             2596..3024
                     /locus_tag="JN701_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HAY7366133.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            3120..3551
                     /locus_tag="JN701_003856"
     CDS             3120..3551
                     /locus_tag="JN701_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366134.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVASGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSNVEAVAEPDEPEEMWTVKRG"
     gene            3544..3987
                     /locus_tag="JN701_003857"
     CDS             3544..3987
                     /locus_tag="JN701_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAY7366135.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGCIKRQMFTKLRTTKYLKTAASADSASVQFEGRVQRIA
                     RIHHYGLSDRVSRKGIYTIYAKRQLLGFNVQSIELIERMFIKNLT"
BASE COUNT          906 a         1017 c         1239 g          845 t
ORIGIN      
        1 ctatgtggtt gaggactacc gctgcgccgc actggtggaa aacatccaga ttagtgattt
       61 cagcgccgcc gcagccgaaa ccggagcgta atccatgagc ctgagtcccg cacggcagca
      121 tcgcctgcgc gttcaggctg aacaggccgt ccgtgagggt ggcagtgttc gccacgcatc
      181 gggctatgac ctgatgctgc tgcaactggc ggaagaccgc cgccgtctca agggcgttca
      241 gtccacggtc aaaaaagcgg aaatcaaagt ggagctgctg ccgaagtacg ccgcctgggc
      301 ggagggtgtc ctggctgccg gaggcgctca acaggatgac gtgctgatgt acgtgatgtt
      361 gtggcgcatt gatgccggag attatgccgg ggcgctggag atcgggcgtc atgccctgcg
      421 tcatggatgg gtgatgccgc tgggcaaccg caacgtgcag accgtgctga cagaggaaat
      481 ggcagatgca gcgcagagcg caatgcttgc cgccaccggc tttgatgccg atctgttgct
      541 gcagacgctg gagctgacag acggtctgga tatgccggac cagtcacggg cacgtctgca
      601 taaagcgatt ggcgcggtcc tgagtgaaag caatccggct tccgccctta atcatctcaa
      661 ccatgcgtta cagctcgatc cccgctgtgg cgtgaaaaaa gacaaacagc agctggagcg
      721 cagactgcgc aatgacagcc gctgacagaa cgtgcccccg cgcacgggcg gcacggggtg
      781 gcgaaaggca cagccacatc aaaaccccgt ccaccgccct ttatttcagg agaaagcagc
      841 atgaagtttg ttgcgccaga acaggcaccg gaacaggcgg aaatcattag aaatacgccg
      901 ttctggcctg atgtggacct gtcggagttt cgcagtgtca tgcgcactga cggcacggtg
      961 acgcagccgc gtttaaagca ggttgcactg tcggcaattt cggaggttaa cgcagagctg
     1021 tatgagtttc gcagacgcca gcagatgctg gggtatgcgt cgctggctga ggttccggcg
     1081 gaacagctgg acgggaaaag tgagcgcatt cagcactatt tcaacgcggt ttactgctgg
     1141 gctcgcgcca tgctcaacga acgtttccag gactatgacg ccacggcatc cggtgcgaag
     1201 cggggcgaag aactggcgga agcaagcggt gatttgtggc gtgacgcccg ctgggccatc
     1261 agccgggtgc aggacgcgcc gcactgcaca gtggagctta tctgatgaaa gtgcgtgcgc
     1321 atcagtatga cacggtggac gcgctttgct ggcgtcatta cgggcgcacg cagggtgtca
     1381 cggagcaggt actgaaggca aatccggggc ttgccgaata cggccccttt ttacctcacg
     1441 ggctgcaggt ggagctgccg gacattccga ccaccaccac cgtgcagacc gtccagctat
     1501 gggactgaat tatgacgctt gagcgaatca gcgcctttat cacgtattgc atcgccgtcg
     1561 tgctggcctg gctgggcgat ttgtccatca aggatgcctc aacgctgggc ggcctgatga
     1621 ttggtgtgct gatgctggct atcaactggt actacaaaca caaagcctac cagcttctgc
     1681 gcgacgggca gatctcgcgg gaggactatg aatccatcaa tcgttaaacg ctgccttgtc
     1741 ggggccgtgc tggctattgc tgccacgctg ccgggttttc agcagcttca cacctccgtg
     1801 gagggactga aactgattgc cgattacgaa ggttgtcgtc tgcagccgta tcagtgcagc
     1861 gcgggtgtct ggaccgacgg tattggtaat acgtcgggcg tcattcccgg caaaaccatt
     1921 acggagcgac aggcagcaga agggctgatc tccaacgtgc tgcgtgtgga gcgggcgctg
     1981 gaaaagtgtg tgaagcaaca gccgccgcag aaggtgtatg acgctgcggt gtcgtttgcc
     2041 ttcaacgtgg gtacgggcaa tgcctgcagt tccacgctgg tgaaattgct caatcagcgg
     2101 cgctgggcgg atgcgtgccg acagttgccg cgctgggttt atgtgaaagg tgtttttaat
     2161 caggggctgg ataaccgccg tgcgcgggag atggcctggt gtttacaggg agcaaactga
     2221 aatgaaaaag aaattaatca gcgggctgtt tctgatgtta tggatggcgc tgttaatcgc
     2281 agcaatggtg tatccgcagg ggatttttcc ggtactggca gcgtccggcg tttgggtagc
     2341 ctgtttactg acatgggcgg taattccggt agcactggct tcgttaatta agaatggccc
     2401 gctctggcag gagttgaggg catctttact gaagacaatt acccgaaaag aaaacgtatt
     2461 tatcagctgg gtgatacgat tgctgattgt cgtcagtctc gcctggacgg ggtgggccat
     2521 taccctggtc ttttatctgc tgaccgttat tgccttctgg atcacccata atcagatggc
     2581 gcaacaggta gccgcatgaa ccggttgctg ctggttgtgc tggcgttatt attggcggcg
     2641 ctgggctggc agacgtggcg gctggctgat gccagccaga ccatcagcac gcaggcagac
     2701 gagctgcaga gcaaaagcca ggcactggca aagagcaaca gccagcttat cagcctgtcc
     2761 attctgactg aaaccaataa ccgggagcag gcgcggctct atgccgaagc agaacagacc
     2821 agtgtactgc tgagacaacg acaacgccgg attgaggaac tgaaacgtga gaacgaggat
     2881 ttacgtcgct gggctgatac tcctttgcct gctgacatta tccggctgcg ggaacgtccg
     2941 gcactcaccg gaggtgcagc ttaccgtcag tggttgtccg cgagtgacgc cgtgtcggct
     3001 ggagcaggca gcgccgcgca ctaacggtga tctgaatgcg ttgctggatg aaacggaggc
     3061 cgcctgggcg gtctgtgcag acaaagtgga catgattatt gcgtgtcagg agcgaaacag
     3121 tgaacaaacc acaatccctg cgccacgccc tcaataaagc agtgccttat gtccgtaata
     3181 acccggacaa actgcatctg tttgtggata acggttcgct ggttgcctcc ggggctagct
     3241 ccatgtcatg ggagtaccgc tacaccctga acgtggtgat tgaagatttc agcggcgacc
     3301 agaatctgct gatggccccg gttttgctgt ggctgcggga taaccagccc gatgcgatca
     3361 ataacccggc gttacgggaa aagctattca cctttgaggt ggatattctg cgcaacgatg
     3421 tctgtgatat cagccttaac ctgcaactga cggagcgtgt gctggtcagc actgacggca
     3481 gtgtgtcgaa cgttgaagcg gtagcggaac ctgatgaacc tgaagaaatg tggacggtga
     3541 aacgtggctg aattgcagaa ggtggacgac tggctgagtg cattattggc gaatctggag
     3601 ccagccgcaa gaagccgcat gatgcgccag ctggcgcagg aactgcgccg gacacagcag
     3661 cagaacatca ggatgcagcg caatcctgat ggcagcagtt atgagccgcg cagggtaaca
     3721 gcacgcagca aaaaggggtg catcaaacgt cagatgttta caaagctgcg caccacaaaa
     3781 tacctgaaaa ctgcagccag cgccgattct gccagcgtac agtttgaagg tagggtgcag
     3841 cgcattgccc gtattcacca ttacggcttg agtgatcgcg tcagtcgaaa aggtatttat
     3901 accatctatg cgaaaaggca gttattaggt tttaatgtcc aatctattga gttaattgaa
     3961 agaatgttca ttaaaaacct aacttgattt ttatttgttt tcaattg
//
LOCUS       DADLTD010000268         3981 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLTD010000268 DADLTD010000000
VERSION     DADLTD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.272"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..679
                     /gene="csgG"
                     /locus_tag="JN701_003858"
                     /pseudo
     CDS             1..679
                     /gene="csgG"
                     /locus_tag="JN701_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(743..1234)
                     /locus_tag="JN701_003859"
     CDS             complement(743..1234)
                     /locus_tag="JN701_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY7366136.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1336..1890)
                     /locus_tag="JN701_003860"
     CDS             complement(1336..1890)
                     /locus_tag="JN701_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7366137.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1914..2651)
                     /locus_tag="JN701_003861"
     CDS             complement(1914..2651)
                     /locus_tag="JN701_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY7366138.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLYSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2706..3644)
                     /gene="ghrA"
                     /locus_tag="JN701_003862"
     CDS             complement(2706..3644)
                     /gene="ghrA"
                     /locus_tag="JN701_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAY7366139.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3878..3965
                     /locus_tag="JN701_003863"
     tRNA            3878..3965
                     /locus_tag="JN701_003863"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3912..3914,aa:Ser,seq:gga)
BASE COUNT         1047 a         1038 c          948 g          948 t
ORIGIN      
        1 ggtatacaac attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc
       61 cactgctgtt ccgcaaagcg ccacggcaat gctggtcacg gcattgaaag attctcgctg
      121 gtttataccg ctggagcgct agggcttaca aaacctgctt aacgagcgca agattattcg
      181 tgcggcacaa gaaaacggca cggttgccat taataaccga atcccgctgc aatctttaac
      241 ggcggcaaat attatggttg aaggttcgat tatcggttat gaaagcaacg ttaaatcggg
      301 cggtgttggg gcaagatatt ttgggatcgg tgcagacacg caataccagc tcgatcagat
      361 tgccgtgaac ctgcgtgtcg tcaatgtgag taccggtgag atcctctcct cggtgaacac
      421 cagtaagacg atactttcct atgaagttca ggccggggtt ttccgcttta ttgactacca
      481 gcgcttgctg gaaggggaag tgggttacac ctcaaacgaa cccgttatgc tgtgtctgat
      541 gtcggctatc gaaacagggg tcattttcct gattaatgat ggtatcgacc gtggtctgtg
      601 ggatttgcaa aataaagcag aacggcagaa tgacattctg gtgaaatacc gccatatgtc
      661 ggttccaccg gaatcctgac agataaaaaa gccggaatta tcatccggca ttttactgtt
      721 taagcgaaag cgtgagtgcc cctcacgctt tatttttgat ttcctgctca cggtgagccg
      781 tctttgcact acgcgccgcc agccacaggc cgctgttttt cattgcgtaa ccaaatacca
      841 gcccaagcgc cagcgaaggt aacaccagtt tccagtcacc ctgcccggca aacgtcgcgc
      901 atgcgcctat aaacgtcccc ggaacaaatg aaagcagcag ctgtttggcc tgaatacaca
      961 tcagaaacgc cacaataccg gttatgacat aaccgagaat ttccagatgt ggtgccagtg
     1021 cactaccgta aataatgacc atcgcccaca ccacgccact aagcagggtt gcagcggaga
     1081 tcgccagccc tttcagccca ccttgcgggc aggcaaagta ggccgtacag cccaggaagc
     1141 ccgcccagct tagtaagccc agggaaacag ccacccatcc ccagataccg gagagaatgc
     1201 ctgttgtgat tgcaatagag agaagtatgt tcatgccgcg catgatagca gaaaacgcgg
     1261 ccatgaatga gatcgagcac acattttatg caacaacaaa ttatctgttg cagttatgag
     1321 tgacgatgat cacatttact cttcagaatc ttcttccagc tcgtcccaca ttgcactaat
     1381 ggcatcacgg gttagcggtg ccatggtgcg ccagaaaggc gtggttgcat gggcctccac
     1441 tttgccgagg aacgcaccac accagggtaa cagatactca ctgaacagtg tttccagtgc
     1501 ttcgctctca tcttccgttg actgatcttc catccaggaa gccgcaagca gcaatgtgcc
     1561 gatgtgatcg gctggcgtat ccgccagtgg catccctcgt tcggaaagaa aagcgcgcac
     1621 ttccgcttcc gttgcgtcct caacccatgc gctacgatat ggcggcacgg cacattcatc
     1681 gccgataaac aacgcattgt aatcggcaga gacttgcgcc atatcacagc ttttctgtaa
     1741 acgcgtcagt aactcatcct gctccagtgg ccagttcgca gccagtttcc cctcacgaat
     1801 cagggtaaac agcggcacca gtaaaggatc ttgcggttgg cggtaataga gcgaacccag
     1861 cacacgacag aggatagaaa actcgttcat taatatattt ccattactag taattaaagg
     1921 tctgcaaatt ccgcaatcgg tgccataccg cgagattcaa ggaagttcag taagcggcgc
     1981 ggagaaacat tcaaaatgcg ctctggcgga aaatctaccg cgtcgaggat tttaagacac
     2041 tcttcaaatt cccccatggt aaacgcggtg tgagaatccg agcctaatgc cacccaacct
     2101 ccagcatcac gtaccgctgc ggctaccgca cgacagttgt cttcactgcc cttacgtgag
     2161 tataaaaatg aagaattatt gatttccagc gccacctgat gtttcgcggc tgcttcagca
     2221 acggctttca catctatttc atatttggga tttccgggat ggcttattat atgcacattg
     2281 ccgctggcga tagtggcgat catcgcttgt gtattggtcg ctttgtcatg tggtgcaaaa
     2341 accggctcat gaaaaccggc aataatcaga tccagcgagt caaacatttt accgctgcaa
     2401 tcaatttcac catcaacatt tttaatgtta gcttcgatgc cgcgcaggat ccctacccca
     2461 tcaaccactc gcggccagat acgcatgtta atgaagtgcc agtgatgcgg agcatcttcc
     2521 atatccgggc catgatcggt gatcgcaaaa agtttaatgc ccttttgttt ggcctgggca
     2581 atgtaatcac tcaatgtgct atatgcatgt gtgctggcaa cggtatgcat atgaaggtcg
     2641 acgggataca taactctctc ctgtattcaa ttcccgccaa ggatagcagg aatcctgatg
     2701 ctttattagt agccgcgtgc gcggtcgact tgcccgcaga ccctctcccc tttttcgagc
     2761 tgggcaatgg tgcgagaaat gtactccaca gcttcagcgg gacgggtaat cgcggcgaca
     2821 tgtggtgtta tcgtcacgcg tggatgttgc cagagcggac tttcaggcgg taagggttca
     2881 cgattaaaaa catccaacat tgcgccttta actttgccgc tatccagcgc cgcgagcagg
     2941 tcatcttcca caacatgaac accacgcgcc aggttgagga gatacgcgcc atccggtaat
     3001 ttttcgagta attgttgatt aataatgccg acggtttcag gggtattcgg taacaaatta
     3061 atcaataccc gacattggct cagaaatgca gacagttctt cccgtccggc aaagctttgc
     3121 acgccaggcc acgatttacg ggttcgactc cagcaacgca gcggaaagcg ccaggtttgc
     3181 agactctgag caactttact gcccagtacg cctgcgccca aaatgccgat ggtaaaatct
     3241 tcccaatgat attcaggcag cggttgccaa tgcgaactat tttgctggat gcgataatcg
     3301 tcaaaacgtc gaaaccaatg cagcacctga ctgacagcat attcctgcat ttgctcgccc
     3361 ataccagtat cttccaggcg aaaaagtgga acagaagggt tcagcatttc agggtgtgcc
     3421 tgtagcttgc tcaaaataga atcaacaccg gccccgagtg cgaacaccgc tttaagatcg
     3481 cgccctgcca gcatttcaac aggaggatgc cagactaaag cataatcagc agagtcatta
     3541 tctccgcttt tccatgctct gactcttgcc tgaggaatag ctttgcgcag tgcctcaatc
     3601 caccattggg tatcgaacgt tgggtgataa aagatgatat ccatactgac ccccgaaaag
     3661 cgttgtgcga aatttattcg cacttatcgt tatgatctac aaaggccacc agcataacaa
     3721 atccgtggtc ggtggcaaaa aaagcagatt tcgcttatta aaaccacaca ttgattgaag
     3781 tttgaataaa cgcgcgattt tttcaaaaag tttgttgacc tcaggtcatg atttccctaa
     3841 attagcgccc gttccagcaa gacaggaaca acaatttggt gaggtgtccg agcggttgaa
     3901 ggagcacgcc tggaaagtgt gtatacggca acgtatcggg ggttcgaatc cccccctcac
     3961 cgccatattt aaagaagagc t
//
LOCUS       DADLTD010000269         3937 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLTD010000269 DADLTD010000000
VERSION     DADLTD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.273"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..787
                     /locus_tag="JN701_003864"
     CDS             <1..787
                     /locus_tag="JN701_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7366140.1"
                     /translation="SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTG
                     VCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDF
                     HDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYD
                     CGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQN
                     GILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(830..1690)
                     /gene="rsmI"
                     /locus_tag="JN701_003865"
     CDS             complement(830..1690)
                     /gene="rsmI"
                     /locus_tag="JN701_003865"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY7366141.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1754..3781
                     /locus_tag="JN701_003866"
     CDS             1754..3781
                     /locus_tag="JN701_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7366142.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3739..>3937
                     /locus_tag="JN701_003867"
     CDS             3739..>3937
                     /locus_tag="JN701_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7366143.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVR"
BASE COUNT          975 a         1043 c         1032 g          887 t
ORIGIN      
        1 cagcctggat attaaaacca cattacaggc aaacgacatt ccaggactca caaccgacca
       61 ggttgtctct gttaacaccc gattcacaca gataaaaagc agcaccgtat attctgctgc
      121 aacccaaacg ggtgtttgcc agggtgacac gtctcgttat ggacccgtta atattggtgc
      181 aaataccacc tttaccctgt atgtcaccaa gccatttctc ggctcgatga ccattccgaa
      241 aacggatatt gccgtcatta aaggcgcgtg ggtcgatgga atgggaagcc cgtctacagg
      301 tgacttccat gatttagtca agttatcgat tcagggaaat ctcaccgccc cacagtcgtg
      361 caaaattaat cagggcgatg ttattaaggt taattttgga ttcatcaatg gtcagaagtt
      421 taccacccgc aatgccatgc cagacggttt tactccagta gactttgata tcacttatga
      481 ctgtggtgat acttcaaaga ttaaaaactc gttgcaaatg cgcatcgacg gtacaactgg
      541 ggtagtagac cagtacaacc tggtcgccag gcgaagaagt tcagacaatg tgcccgatgt
      601 cggtattcgt attgaaaatc tcggcggcgg agttgcaaat attccttttc agaacggtat
      661 ccttcccgtt gatccttccg ggcatggcac cgtcaatatg cgcgcctggc cagttaatct
      721 ggtcggtggt gagctggaaa caggaaaatt tcagggcaca gccaccatta ccgtcatggt
      781 gcggtaaaag aagacgttgc gttttgatta acgcaacgct tcggcactgt tacccctgct
      841 gctccagcgc atacttatac agcgcatttt tcttcacgcc atgaatttct gcgaccagcg
      901 ccgccgcttt tttcagcggc agttctgcct gtagcagcgc cagcgtgcgc agggcatcgg
      961 cgggtaagtc ttcttcctgt gctttatgac cttcgacaat cagcaccatt tcgcctttgc
     1021 gacggttttc atcttccttt acccacgcca gcagttcgcc aacgggcgcg ccgtgaatgg
     1081 tttcccaggt tttggtcagc tcacgcgcca gaaccacgta gcgggattcg cctaataccg
     1141 caacgatatc ttccaggcta tctaacagac ggtgggtgga ttcataaaaa atcagcgtgc
     1201 gcggctccgc ttcaatggct tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa
     1261 agccttcgta acagaaacgg tcagagggta aacccgcggc gcttaacgca gtgatagcag
     1321 cacacggccc gggtagcggc accacgcgaa tccccgcttc acggcaggta cgcaccagat
     1381 ggtagccagg atcgttaatt agcggcgttc cggcatcgga aaccagcgca atgttttgcc
     1441 cctcttgcag cttcgccagc agcgtttcgg ctttttgttg ttcgttatgg tcgtgcagcg
     1501 caaacaaccg ggcattaatc ccaaaatgtt gcagcaataa accggtgtga cgagtatcct
     1561 cggcggcaat cagatcaacg gcctgtaata cctctaacgc acgctgggtg atatccgcca
     1621 gattgccgat tggcgtcggt acaatgtaaa gctggccctg agaattatcc gccgattggt
     1681 gttgtttcat tgtgtcgtcc gtattgccga tttaatattg agcattgcgt aaaaaaatat
     1741 cactggatac attatggtac cctcaacatt ttctcgtttg aaagccgcgc gttgtctgcc
     1801 tgttgttctg gcagccctga ttttcgccgg ttgtggcacc catactcccg atcagtccac
     1861 tgcttatatg cagggcacgg cgcaggctga ttctgccttt tatcttcagc agatgcagca
     1921 aagctctgat gataccagga tcaactggca attactcgcc attcgtgcac tggtgaaaga
     1981 aggtaaaacc gggcaggcgg ttgagttgtt taaccaacta ccgcaagaac tgaacgattc
     2041 tcagcgtcgc gagaaaacac tgctggcggt agagattaaa ctggcgcaga aagattttgc
     2101 tggcgcgcaa aacttgctgg cgaaaatcac acctgccgat ttagaacaaa accagcaagc
     2161 gcgttactgg caggcaaaaa tcgatgccag ccaggggcgt ccttccattg atttactgcg
     2221 cgcgttaatt gctcaggaac cgctgctcgg cgcgaaagaa aaaaagcaga atattgatgc
     2281 cacctggcag gcgctctcct ccatgactca ggaacaggcg aatacgctgg tgatcaacgc
     2341 cgacgaaaat attctgcaag gctggctgga tctgcagcgc gtctggtttg ataaccgtaa
     2401 cgatcccgat atgatgaaag ccgggatcgc cgactggcag aaacgttatc cgaacaatcc
     2461 gggcgcgaaa atgctgccaa cgcagttggt taacgtaaaa gcgtttaaac cagcctcgac
     2521 caacaaaatc gccctactgt tgccgctgaa tggccaggca gcggtatttg gtcgcactat
     2581 tcagcaaggc tttgaagcgg cgaaaaatat cggcactcag ccagtggcgg ctcaggtagc
     2641 tgccgcagac gtagcagaac aacctcagcc gcaaactgcg gatggcgttg ccagcccggc
     2701 acaagcctcg gttagcgatc tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag
     2761 cgccccggcg acaagcaccg cagcggtaag cgcacccgca aatccatccg cagagctgaa
     2821 aatctacgat acctcatcac aaccacttag ccagatctta agccaggttc agcaggatgg
     2881 cgcgagtatt gtggttggtc cgttgctgaa aaataacgtt gaagagttgc ttaagagcaa
     2941 caccccgctg aacgtgctgg tactgaacca gccggagaat atcgaaaacc gcgtcaatat
     3001 ttgttacttc gcgctttcac cggaagacga agcgcgcgat gcagcgcgtc atattcgtga
     3061 ccagggtaaa caagcgccgc tggtgctgat cccacgcagt tcattgggcg atcgcgtagc
     3121 caatgcgttt gcgcaggagt ggcagaaact gggcggcggc accgttctgc aacaaaaatt
     3181 tggttccacc agcgaattac gcgcgggtgt taacggcggt tctggtatcg ctttaacggg
     3241 taccccgatt actcccagag cgacaaccga ctccggcatg acgaccaaca atccaacgct
     3301 gcaaaccacg ccaaccgatg accagttcac caataatggc ggtcgtgtcg atgcggtgta
     3361 cattgtggca acgccgggtg aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg
     3421 tagccagagc ggtgcaatgc tgtacgccag ctcccgcagc gcacaaggca ccgcaggccc
     3481 ggatttccgt ctggagatgg aaggtttgca gtacagcgaa atcccgatgc tggcgggcgg
     3541 taatctgccg ttaatgcagc aggcactcag cgcggtgaat aacgattatt cactggctcg
     3601 catgtatgcg atgggcgtcg atgcctggtc gctggcaaat catttctcac aaatgcgcca
     3661 ggttcagggt tttgaaatca acggtaatac cggaagcctg acggcaaacc cggattgcgt
     3721 gattaacagg aagttatcat ggctacagta ccaacaaggt caggtagtcc ccgccagtta
     3781 accaccaaac agaccggcga tgcgtgggaa gtacaagcgc gtcgctggct ggaaggcaaa
     3841 ggactgcgat ttgtcgccgc taacgtgaac gagcgtggcg gtgagatcga tctgataatg
     3901 cgtgaaggct ggaccaccgt ttttgtcgag gtgcgtt
//
LOCUS       DADLTD010000270         3928 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLTD010000270 DADLTD010000000
VERSION     DADLTD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.274"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..435)
                     /locus_tag="JN701_003868"
     CDS             complement(<1..435)
                     /locus_tag="JN701_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7366144.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVE"
     gene            complement(537..1385)
                     /gene="yiaJ"
                     /locus_tag="JN701_003869"
     CDS             complement(537..1385)
                     /gene="yiaJ"
                     /locus_tag="JN701_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY7366145.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            1586..2584
                     /gene="yiaK"
                     /locus_tag="JN701_003870"
     CDS             1586..2584
                     /gene="yiaK"
                     /locus_tag="JN701_003870"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY7366146.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            2596..3064
                     /locus_tag="JN701_003871"
                     /pseudo
     CDS             2596..3064
                     /locus_tag="JN701_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
     gene            3182..3655
                     /gene="yiaM"
                     /locus_tag="JN701_003872"
     CDS             3182..3655
                     /gene="yiaM"
                     /locus_tag="JN701_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HAY7366147.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            3658..>3928
                     /locus_tag="JN701_003873"
     CDS             3658..>3928
                     /locus_tag="JN701_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY7366148.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKP"
BASE COUNT          967 a          992 c          953 g         1016 t
ORIGIN      
        1 ctcgacatcc atgcactgca acgcctttgt cgggcaggct tcaacacagg ccggccccgt
       61 ctcccgatgc cagcacagat cgcacttaat cgccctcgcc tttttgcgcg aagaaacgac
      121 ctccatcgca ccaaacgggc aagccagcat acagctttta cagccaatgc aacgtgtttg
      181 ttcaacgaaa atatgcccat gttcgcggct tatcgcgtca acagggcaga cattcgcgca
      241 cggtgcatct tcacactgat gacaggctac tgccgtggtc cagctgtggt ctttaatgac
      301 acgaatacgg gaaataaact cgtctggtga caacgcagcg caatcctgat tctcctgatg
      361 cgacactgcg caagccactt cacaggtacg gcaaccgata catttcgtcg catccgcaat
      421 aataaaccgg ttcatgatca ctcctttgca ctaaaacagc agagtgccag agagcgcgtc
      481 cctactgctt tgatccaacg aaggaaatgt taaagttttg gcacttgtcc aaagcgttat
      541 gtgatcgcgc ccaggtcatc cctgacagta aatcccagtt cattagaaat agcctgcgcg
      601 gtttcacgca gtggtttcag gagatttttc tctcccacct gtttcagacg tgatgtcgaa
      661 agcgaaatcg acacggcgta cggcacccgc ccatgaatat caaacaccgg aacagcaata
      721 caggagacgc cgagttcgtt ttcttctctg tccatcgccg ctccgctttc acgaatgtgc
      781 gccagttcgt cgaacatcgc gggcagctcg gtaatggtat tgcgggttaa cggctggatc
      841 tcatgctgat ggttttccca gtatgacttc acgtagtccg ggtgaccaaa cgccatgtag
      901 atcttgccca ttgcggaaca gtagagcggc atatgctggc caatataggc acgggttcgc
      961 agcatcccgg ttgtgggttc cagcttataa atcaaaatag cgtgatcgtc ttcgcggctg
     1021 gagaagttaa tggtttcacc agtggcgatg ttcagtgcct caagatgcgg agcggcgata
     1081 tgaatgatat tcagcgaaga cagcgccttc tgcccgacgg caataaattt ggtagtcagg
     1141 cggtaactcc ctgcggcggg cgcggtggtc acatagccgc aggactgtaa tccctgcaat
     1201 aagcgatgga cggtactctt atttaaacca gccagctccg aaagatgcgc caacggacaa
     1261 ccgtttggat agttgctcaa aatctcaatc agcatcaacc cgcgaaacag actctggctt
     1321 ccggctggac gctctttttc ctgcgccatc tcgttctctt tttttcccat cacttctttc
     1381 cccattttgt cgcgtcctga tggtagcgca aagtgtgccg tagttcacga tctcgacaga
     1441 taatttataa ccacttgatt tttatgtctt ttgaaattca tcaatcagat tgctttgtta
     1501 aaaagtgatc gatatatttg aaatcaagtt tcgcatattg aaattttaag ccaaaaaagc
     1561 gatcaaaaaa acaaggaagc ctgggatgaa agtgacattt gagcagttaa aagcagcctt
     1621 taatcgggcc ttaatttcac gcggcgttga cagcgaaacg gctgacgcct gtgcagagat
     1681 gttcgcccgc accaccgaat ccggcgttta ttctcacggc gttaatcgtt tccctcgttt
     1741 cattcaacaa ctggaaaacg gcgatatcat tcctgatgcc caacccaaac gtataaccag
     1801 cctcggcgca attgaacagt gggatgccca gcgttcgatc ggcaacctga cggcgaaaaa
     1861 gatgatggat cgcgccattg aactggctgc cgatcacggt attggtctgg tggcacttcg
     1921 taatgccaac cactggatgc gcggcggcag ctacggctgg caggcggcgg aaaaaggcta
     1981 tattggcatt tgctggacca actccatcgc cgtaatgccg ccgtggggcg caaaagagtg
     2041 tcgcattggc actaacccgc tgatcgtcgc cattccttcc acgccgatca ccatggtcga
     2101 tatgtcgatg tcgatgttct cttacggcat gttagaagtt aaccgcctgg cgggccgtca
     2161 gctcccggtc gatggtggct ttgatgatga gggcaatttg accaaagaac ctggcgttat
     2221 cgagaagaat cgccgcattt tgccgatggg ctactggaaa ggttctggca tgtcgattgt
     2281 gctggatatg atcgctactc tcctttccga cggcgcatcg gttgccgaag tcacccagga
     2341 caacagcgac gaatacggca tttcacaaat ttttattgcc attgaagtgg acaagcttat
     2401 cgacggtccc acccgcgatg ccaagctgca acgcatcatg gattacgtta ctaccgctga
     2461 acgcgctgac gaaaaccagg ccatccgctt acccggccac gaatttacta ccctgctggc
     2521 cgaaaaccgc cgtaacggca tcactgttga tgacagcgtg tgggccaaaa tccaggcgtt
     2581 atgaggagat aagtcatgat ttttggacat atcgcgcagc ccaatccgtg ccgtttgccc
     2641 gccgccattg aaaaggcgct cgattttctg cgcgctacca acttcaacgt cctggagccg
     2701 ggcgttgtcg aaatcgacgg caagaatatt tatgcgcaaa ttatcgattt aaccacacgc
     2761 gaagcggtgg aaaaccgtcc ggaagtgcat cgtcggtata tcgatattca gtttctggcg
     2821 tggggtcaag agaaaatcgg cattgctatt gatacaggaa ataataaagt cagcgaatca
     2881 ctattagagc agcgcgatat tattttttat catgtcagtg aacatgaatc atttatagaa
     2941 atgataccgg gcagctacgc tattttcttc ccgcaggatg ttcaccgacc aggttgtatt
     3001 ctgcaaacag gcctctgaga tacgaaaaat tgtggttaaa gtcgcattaa cagcgctgaa
     3061 ttaatcaaat aatcgtctac gccagaacga ctgggtaatg ttattgctct aactatatta
     3121 ataatttagg ttgctgcaca tgcaacttga attatgacag acatagcctc aaggaatagc
     3181 tatgaaaaaa atacttgaag taatactggc gattaatctc gccgtacttt cctgtattgt
     3241 gtttataaac attattttaa gatatggttt tcagacaagc attttatctg tagatgaatt
     3301 gtcacgttat ttatttgtct ggttaacatt tattggcgcg attgtggctt ttatggataa
     3361 cgcccacgtt caggtcacct ttctggtgga gaaactctcc cccgcatggc aacgacgagt
     3421 cgctctggtt acccattcct taatcttatt tatttgtggc gcactggcct ggggcgcaac
     3481 actaaaaaca attcaggact ggagcgatta ttcaccgatc ctcggtttgc ccatcggcct
     3541 gatgtatgcc gcttgtctgc ccaccagcct cgttatcgca ttctttgaac tgcgtcattt
     3601 atatcaactc atcacgcgta gcaattctct aacgtcgcca ccgcaaggag cttaatcatg
     3661 gctgtgctga tttttctggg ctgtctgttg ggtggtattg ctatcggttt gcctattgcc
     3721 tgggcactgt tgttgtgcgg ggcggcgtta atgttctggc tggacatgtt tgatgtccag
     3781 atcatggcac aaacgctggt gaacggagcc gatagcttct ccctgctggc gattccgttc
     3841 tttgtgctgg cgggtgaaat catgaatgcg ggcggcttgt caaaacgcat tgttgacctg
     3901 ccgatgaaac tggtggggca taaaccag
//
LOCUS       DADLTD010000271         3917 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLTD010000271 DADLTD010000000
VERSION     DADLTD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.275"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(313..732)
                     /locus_tag="JN701_003874"
     CDS             complement(313..732)
                     /locus_tag="JN701_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7366149.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            812..1066
                     /locus_tag="JN701_003875"
     CDS             812..1066
                     /locus_tag="JN701_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7366150.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            1063..1488
                     /locus_tag="JN701_003876"
     CDS             1063..1488
                     /locus_tag="JN701_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY7366151.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1560..2630
                     /locus_tag="JN701_003877"
     CDS             1560..2630
                     /locus_tag="JN701_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAY7366152.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            2671..3093
                     /locus_tag="JN701_003878"
     CDS             2671..3093
                     /locus_tag="JN701_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7366153.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            3090..3395
                     /locus_tag="JN701_003879"
     CDS             3090..3395
                     /locus_tag="JN701_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY7366154.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            3382..3837
                     /locus_tag="JN701_003880"
     CDS             3382..3837
                     /locus_tag="JN701_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7366155.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSAPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT         1072 a          844 c         1051 g          950 t
ORIGIN      
        1 atcgatagta tccatgctga cctcatttcc ccttaacgcc ggggtagcgg aacaaaaacc
       61 tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc ttacgcctcg ggctggctac
      121 ttaacccctg accactgcct ggtaactcga agtattgccc tgcattctgt ggggcggggt
      181 gggtggcagg tatataatgt actttgcgtt cattgttgta aagtgcttat ggtacatttt
      241 gtgtgtaaaa aaatgagatg ggataaagtg aagcacaaac ccggaggaag gcgctaccgg
      301 atttatgctg gtttaagagg ctttttgttt tttctttcgt gctaactctt cgtaaattgc
      361 attgtacttc tgttttttct cttcaagagt ttttaaaagt tcatctgtct cactgtcagg
      421 gagctcgtcc agaaggtcaa tgatgatttt ttgtcttgga tttaactcct gatagaaacg
      481 tacctgtcca ctttcttctg tatcctctcc caaaagatag gttggtgttg ttcctattag
      541 tgttgctaat tcccttaatt tctcccggcg aggaattgtt tcgccattaa accatttgct
      601 aaccgctttt ggtgttaatt tcattcgacg ggcaatttct gcctgccttc catgttgttc
      661 ataaccagcg ttttcacagg ctagcgcaag cctactggcg aactctttac gcgctttatc
      721 ttcatgaacc ataagttcaa tgatattcgc tcttgaatgt actgtcagtt ctgttatagc
      781 atgtactcaa agttcacatt gtgagggtga tatgaaccag aaaacacttg aagatgtaat
      841 caaaactgtt cgcgttgctg ttgtggccga cgtttgtggt gtcagccaaa gagcaatcta
      901 taaatggatg gataacggaa aattgcctcg cacagaatat accggcgaaa caaattacgc
      961 tgaaaaaatc gctcttgcat caaacggatt attttctgcc gatgcaattt taactattgg
     1021 caggaataaa actactacga aaaagctgat gggagttgat tcatgaaaat caagcatgaa
     1081 cacatccgca tggcgatgaa tgcctgggcg catccggacg gcgaaaaagt accggctgcg
     1141 aaaattacca aagcgtattt cgagctggga atgacgttcc cggaactgta tgacgacagc
     1201 catccggaag ccctggctcg caatactcag aaaattttcc gctgggtgga gaaagacacc
     1261 cctgatgcgc ttaaaaaaat tcaggcgttg ttaccagcta tcgaaaaagc aatgccacct
     1321 ctgctggtgg cccgaatgcg cagccatagc tcagcctatt ttcgggaact agtggagacg
     1381 cgggaacgac tggtgagaga cgctgatgat tttgtcgcag tggcgatcgc tggtttcaac
     1441 cagatgaatc gtggtggccc tgcaggaaat attgtggctg tgcattgact cgcaatattc
     1501 ataccggatc acttccggca atttgtgagt aaaaagattc ggtatcaaaa gaggtgagta
     1561 tggctaacgc ctggctcaga ttatggcatg acatgccaaa tgaccctaag tggcgaacaa
     1621 ttgccagggt gtcagggcag ccaattgcaa cagtgatggc agtgtatatc cacctcctgg
     1681 tgagcgcgtc acgaaatgtc acgcgaggtc acattgatgt cacgacagaa gatttggcaa
     1741 gtgcgctcga cgtgacagaa gaggtaattg attcaatttt gcagacgatg caggggcggg
     1801 tacttgatgg tgatttaatc actggatggg aaaaacgcca ggtgctgaaa gaggacaacg
     1861 gcaatatttc gcaaaccgca aaatctcctg cagagcgcaa gagggcgcag cgagagaggg
     1921 aaagaaagcg ggaacaaaat ggcgattgtc acggcgcgtc acgatatgtc acgcacatgt
     1981 cacgacgagt cacgacagat aaagatacag ataaagatac agatcaagaa gatcaaaaca
     2041 ctatggtcca tggcgtaaaa aacgccacga accaggcagg ggatgttcag accgtcaatc
     2101 ctggtcagcc agcaggcacg acaccggaag ccgattcagc gtatgcgctg aaagccgatt
     2161 cgggcgctgt gcagcaggcg atgaccgcaa gtccggagca atcacaccaa ctgcagcagc
     2221 ctgaagccga ttccgccatt cagcgggaag ccgatcgggt agtcccggaa aacaccgggc
     2281 agtctgtggg acgagtggat tatccggatg tgttcgaaca ggtctggcgg gagtacccgt
     2341 tgcgtgccgg ggcaaacccg aagaaatccg ctttcagtgc ctggaaggcc agattacgcg
     2401 agggggtgcc accagaggcc atgctggatg gcgtgaggcg ttacgcaaga tacctggcgg
     2461 ctaccgggaa aacgggaacg gaatttgttc agcgagcgac gacgtttttt ggaccggacc
     2521 ggaattttga gaacccctgg ttgctcccgg taagcggcac gaacaaccag cgttgtgtga
     2581 atcatatttc tgaaccggat aacgaaattc cgccgggctt cagggggtaa gtgttaattt
     2641 ctggtcatga ggtaattttc aggagggctt gtggcaaaag tttttacaca agaagagcgg
     2701 gaaaaaatta aagggcaggt tcttgaactc gtacgccaga gtgggcgcga gacgttacga
     2761 caactggaag ctaaaactgg ggcaacaaga tatctgatga gcgttctggc cagagagctg
     2821 gttgccagtg gcgatgtata caactctggt tacgggttat tcccgtctga acaggcgcgt
     2881 aaggactggc aaaatgcccg taaaaagctc tcaagggcaa agctgaagaa accatctgcg
     2941 gttgatccgg accttatctg gtcattacct gacggagaaa tacgtcgcta cgacagtcgc
     3001 ctaaacataa tctgtcgcga gtgccggaag agcgaagtta tgcagcgcat attgtcgttt
     3061 tatcagggta attttcagga ggtgatggcg tgagggtgag agtctatatc gccggtccaa
     3121 tgaccgggta taaaaatttc aaccgtgagg cgtttcacaa tgcggaagag gaactgaaac
     3181 gggaagggca taccgtctta aacccggcag tacttccgga cgggctgaca cagccgcagt
     3241 acatggatat ctgcatggcg atgattcgtt gtgtggatgc gatttacatg ctgaaaggct
     3301 ggcagcggtc agcaggcgct aaggcagaac tggcgctggc ggagaaactg gggcatgcgg
     3361 tgatttttca ggagacagcc agtgtgcaag actaattacc aggcattacg tgaacgttat
     3421 tcaccaattc aggtgccgga atgtcctgtt tgtggcgatg aaatgtcgat acagcgcata
     3481 ttttccagaa cgcatattgt gtatgcctgc acaggtgagg gggatgatgg atattttaaa
     3541 actggtcgaa cttttgcgga tgagcattac ctgaaatcgc gcgtaaccgt cgttgatgtt
     3601 agcgctcctg acgtactggc tttactgaaa gagctggaag ttaaagacaa acgcattgca
     3661 gaattgacag atgcgcttac acaaatgatt aatgcgcaca aaaccacaat tcgttttggt
     3721 catgaacgca taactgaatg tggtggtgat tgcgactcgc cggaaaagat gatttcagaa
     3781 aatccggata tcagaatggc agaggctgtt ttgagagcag gaataaaaac tgaataatta
     3841 aatttagcac agcaaataaa atttaatcct taaccggagg gatttctgca ccctcagaac
     3901 atcaggaggc cgcctga
//
LOCUS       DADLTD010000272         3851 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLTD010000272 DADLTD010000000
VERSION     DADLTD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.276"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..439
                     /locus_tag="JN701_003881"
     CDS             <1..439
                     /locus_tag="JN701_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7366156.1"
                     /translation="FTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVR
                     ISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIA
                     EGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            447..1775
                     /locus_tag="JN701_003882"
     CDS             447..1775
                     /locus_tag="JN701_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7366157.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="JN701_003883"
     CDS             complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="JN701_003883"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY7366158.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3360..3743
                     /gene="bssR"
                     /locus_tag="JN701_003884"
     CDS             3360..3743
                     /gene="bssR"
                     /locus_tag="JN701_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY7366159.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
BASE COUNT         1006 a          857 c          950 g         1038 t
ORIGIN      
        1 ttttaccacc atcatgaaag aaaatggcct gaaggcgaac agcctctgtg ttgagatcac
       61 tgaaaccgtg atcgagcgaa ttaatgaaca tttttatctc aatattgaac aactgcgtaa
      121 acaaggggta cggatatcga ttgatgactt tggcaccggt ttgtcaaacc tgaaacgttt
      181 ttatgaaatt aatccagaca gcataaaggt ggactcgcaa tttaccggcg atattttcgg
      241 tactgcggga aaaattgtgc gcattatttt cgatctggca cgctataacc ggatcccggt
      301 gattgcggaa ggcgtagaga gcgaagacgt tgcacgcgaa ttaattaaat taggatgtgt
      361 tcaggctcag gggtatctgt accagaaacc tatgccattc tccgcctggg ataaaagtgg
      421 aaaattagta aaagagtagt ttacgtatgt ccagaatcaa taagttcgta cttacagtca
      481 gtctgctgat ttttatcatg atttcagcag ttgcctgcgg gatctacact caaatggtaa
      541 aggaacgggt gtatagcctg aaacagtccg ttattgatac tgcttttgcg gtggcaaata
      601 ttgctgaata tcggcgtagc gtggcaattg atcttatcaa cacgctaaat cccactgagg
      661 aacagctgtt ggttggtttg cgcacagctt acgccgactc ggtttccccc tcttatttgt
      721 acgatgtcgg tccttatctg atttccagtg acgaatgtat tcaggtaaag gagttcgaga
      781 aaaattattg tgcagatatt atgcaggttg tgaagtatcg acatgtcaaa aatacagggt
      841 ttatctcttt tgacggtaaa accttcgtct attacctcta tccggtaact cacaatcgta
      901 gtctgatatt tttgcttggt ctggagcgtt tttctttact gtcaaaatcg ctggcgatgg
      961 acagcgagaa cctgatgttc tctctattta agaacggtaa accggtgacc ggtgatgaat
     1021 ataatgctaa aaacgccatc ttcaccgttt cggaagcgat ggagcacttc gcctatttgc
     1081 cgaccggatt gtacgtattt gcgtataaaa aagatgttta tttgcgggtc tgtacattga
     1141 ttattttctt tgccgcattg gtggctgtga tatcgggtgc cagttgcctc tatctggtac
     1201 gcagagtgat taatcgtggt attgtggaga aagaagccat cattaataac cattttgaac
     1261 gcgtactgga tggcgggctt ttcttttcgg ctgccgatgt caaaaaactc tacagtatgt
     1321 ataactcggc gttcctggac gacctgacca aagcaatggg cagaaaatcc tttgacgaag
     1381 atttaaaagc gctgccggaa aaaggcagtt atttgtgcct gtttgacgtc gataaattca
     1441 aaaacattaa cgacaccttc ggtcatttgc tgggcgatga agtgttgatg aaagtggtga
     1501 aaatccttaa atcacagatc ccggtagata aaggtaaaat ctaccgcttc ggcggtgacg
     1561 aatttgcggt gatttatacg ggtggaacgc tggaagagtt gctatcgatt ctaaaagaaa
     1621 tcgttcattt ccaggtggga agcattaatt taagtaccag tatcggtgta gcgcattcaa
     1681 atgaatgtac caccgtcgaa cgcttgaaaa tgctggcgga tgagcggctg tataagagta
     1741 agaaaaacgg cagggctcag attagctggc agtaatcatt attcgcaggc cggacaaatg
     1801 attttgcccg gcctgaatta attaaacccg gctaccccac aaatcgtact catcggcgtg
     1861 ctcgactttc acacgcagga tatcacccgg cttaacgttg gtttcaccat tgagataaac
     1921 cgcgccgtcg atttccggtg catctgccat gctgcgacca atcgcgcctt cttcgtccac
     1981 ttcgtcgata atcaccagaa tttcacggcc cactttctct tgcaggcgct cggcggaaat
     2041 ctgctgctgc agctgcatga aacggttcca gcgttcttct ttaacttctt ccggaacctg
     2101 gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg tatttaaagc agccaacgcg
     2161 atccagacgc gcttctttca ggaagtcgag tagcatctgg aaatcttctt ctgtctcgcc
     2221 agggaagccg acaataaagg ttgagcgtag ggtcagttcc gggcagattt cgcgccactg
     2281 tttgatgcgc gccagttggc gatctacaga acccggacgc ttcatcagtt tgagaatgcg
     2341 cgggctggcg tgctgcaacg gaatgtccag atacggcagg attttgcctt ctgccatcag
     2401 tgggatgacg tcgtccacat gcggataagg gtaaacgtag tgcagacgtg tccagatccc
     2461 cagtttcgat aactgttcgc acaggctgac catgctggtt tttaccggct cgccgttgtg
     2521 gaagccagta cgatgtttaa catcaacgcc ataggcggaa gtatcctgcg agatcaccag
     2581 aatctcttta acgcccgcat ctaccagacg tttcgcttca cttaacactt cgccaatcgg
     2641 acggctcacc aggtcgccgc gcatagacgg aataatgcag aaggtgcagc ggtgattaca
     2701 gccttccgaa attttcagat aggcataatg acgcggcgtc agtttcacac cttgttctgg
     2761 caccaggctc aggaatgggt tgtgtttcgg ttttggcacg tagtgatgaa cgtgctccag
     2821 aacctgctca tagctatgag gcccggtgat ttccagcact ttcgggtgga cttcgcggat
     2881 ctgatcttct tttgccccca gacaaccggt cacaattacc ttgccgtttt cattcaacgc
     2941 ttcaccaatg gcttccagtg attcttgtac cgcgctgtca ataaagccgc aggtgttgac
     3001 gatcaccatg tccgcatcgt catagctcgg taccacgtca taaccttcag tgcggagttc
     3061 ggtgagaata cgctctgaat cgacaaggtt tttcggacag ccaagggaaa caaagccgat
     3121 tttcggctgg ggagttactt tgctcatagc ttaaaaaata ttcagttaca ggaaaggtca
     3181 gggcagggat tctacagagt tctggataaa atttgtatcg caatctcatt cgctggcgga
     3241 ggcgaaggaa atgtaaattt tgttaattcg gcgtgaagaa ttgatcctgg acagcatttt
     3301 gctcaaaaaa tagccatact atttaattgc aacaaggctg gaaagaggag gatcgaagta
     3361 tgttcgttga cagacagcga atcgatctgc tgaaccggtt gatcgacgca cgcgttgacc
     3421 tcgctgcata cgtgcaactg aggaaggcaa aaggatacat gtccgtcagc gaaagcaatc
     3481 atctacgaga taactttttt aaactgaatc gcgaactgca cgataaatcg ctgcggttga
     3541 atcttcatct ggatcaggaa gagtggagtg ctcttcatca tgctgaagaa gcattagcga
     3601 cagccgcagt atgtttgatg agtgggcacc atgattgccc gactgttatt accgtcaacg
     3661 ccgataagct tgaaaattgt ctgatgagct taacgctgag tatccagagc ctgcagaagc
     3721 acgccatgct tgagaaggcc tgaaaactaa gggggagaaa gcgtctcccc cttcatgttt
     3781 aagtttttgt aaaaatgaat ttgttatctc ctccactgac tacgctttaa gccagagtca
     3841 atccggaggc g
//
LOCUS       DADLTD010000273         3817 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLTD010000273 DADLTD010000000
VERSION     DADLTD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.277"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            17..253
                     /locus_tag="JN701_003885"
     CDS             17..253
                     /locus_tag="JN701_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C TorY domain protein"
                     /protein_id="HAY7366160.1"
                     /translation="MNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALA
                     TLSEEGIKALTLNGEWQAGEFSASNMVNRNTGCW"
     gene            193..1575
                     /locus_tag="JN701_003886"
                     /pseudo
     CDS             193..1575
                     /locus_tag="JN701_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(1740..2711)
                     /gene="cmoB"
                     /locus_tag="JN701_003887"
     CDS             complement(1740..2711)
                     /gene="cmoB"
                     /locus_tag="JN701_003887"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY7366161.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2708..3451)
                     /gene="cmoA"
                     /locus_tag="JN701_003888"
     CDS             complement(2708..3451)
                     /gene="cmoA"
                     /locus_tag="JN701_003888"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY7366162.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(3492..>3817)
                     /locus_tag="JN701_003889"
     CDS             complement(3492..>3817)
                     /locus_tag="JN701_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366163.1"
                     /translation="RLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMNG
                     AETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWEL
                     VFSLR"
BASE COUNT         1009 a          952 c         1009 g          847 t
ORIGIN      
        1 aggcgagctg gctaccgtga atcctggtac cgatctcacc gtcgttgatg ccagtggcaa
       61 acagccgatc gttctgttgc agggttatca aatgcagggc agtgaaaaca cgctctacct
      121 ggcggcaggt caacggctgg cgctagccac attaagtgaa gaaggtatca aggcgctcac
      181 gctaaacggg gaatggcagg ctggggaatt cagcgccagc aatatggtga acagaaacac
      241 tggatgctgg tgacgctggc ggcggtgtta gggcagattg gtacaccggg cggcggtttt
      301 ggtttctctt atcattactc caatggtggc aacccgacac gtgttggcgg cgtgctgccg
      361 gaaatgtctg cggcgattgc cggacaggcc agcgaggccg ctgatgatgg cggaatgagg
      421 gcgattcctg ttgcccgtat cgtcgatgcg ctggaaaatc ctggcggcaa atatcagcat
      481 aacggtaaag agcaaacgta tccgaatatc aaaatgatct ggtgggcggg tggcggtaac
      541 tttacccatc accaggatac taatcgtctg attaaagcat ggcagaaacc ggagatgatc
      601 gtcgtttctg aatgctactg gaccgcagct gctaaacatg cagatatcgt attaccgatc
      661 accacatcgt ttgagcgcaa tgacttgacg atgaccggtg attacagcaa ccagcatatt
      721 gtgccgatga agcaggctgt cgctccgcaa tttgaagcgc gaaacgattt tgatgtgttt
      781 gccgatcttg cggaattact gaaacctggc ggaaaagaga tctataccga aggtaaagat
      841 gaaatggcgt ggctgaaatt tttctatgat gccgctcaga aaggtgcccg tgcgcaacgc
      901 gtgactatgc cgatgtttaa tgccttctgg cagcaaaata aactgatcga aatgcgccgc
      961 agcgagaaga acgaacagta cgttcgttat gctgatttcc gcgccgatcc ggtgaaaaat
     1021 gcgctgggta cgccaagcgg caaaattgag atttactcca aaacgctgga aaaatttggc
     1081 tataaggatt gcccggcaca tccaacctgg cttgcgcctg atgagtggaa gggtaccgcc
     1141 gacgagaagc agttgcagct tctgaccgca catccggcac accgtttaca tagtcagctt
     1201 aactatgcgg aactgcgtaa aaaatatgcg gttgcagatc gtgaaccaat cactattcat
     1261 accgaagatg ctgctcgctt tggtattgcg aatggtgatc tggtgcgtgt gtggaacaaa
     1321 cgtggtcaga ttctgacagg cgcggtggtg actgacggga tcaaaaaagg cgtggtatgc
     1381 gtgcatgaag gtgcatggcc agatctggaa aatggcttgt gtaaaaacgg cagtgcgaac
     1441 gtgttaacgg cggatatccc cagctcgcag ctggcaaatg cctgtgccgg taactctgcg
     1501 ctggtgtata tcgaaaaata tacgggtaac gcaccgaagt taacggcatt tgataagcct
     1561 gctgttcagg cgtaagaagt gaatagcgcc cggaaacggg cgctgaggtt atgcaaacgt
     1621 aaaactgcct gatgcgctat gcttatcagg cctacatagt ccctgcaata gattgaatta
     1681 gcaagggttt gtaggccgga taaggcattc acgctgcatc cggcagtaat accagacctt
     1741 tacggcttgc gcgcaatcag caccgcgcgt ttaggcgcag gataaccttc aactgtctta
     1801 ctcggatcat gtgggtcgag gaaatcggac agagattcgg taaccatcca ttcagtgcgt
     1861 cgctgctctt cagtagtggt aacgcacaca tccacaacgc ggatatctac aaaaccacac
     1921 ttcttcagcc agtttttcag cgccagcgcg gaagggatga aatagacatt acgcatttgc
     1981 gcgtaacgat cgcctggtac cagcaccgtg ttctcatcgc catcaataac cagcgtttcc
     2041 agcaccagtt caccctcatt caccagttga tctttcaact gccagagatg ctccagcggc
     2101 gaacggcgat ggtaaagcac gcccatcgaa aagacggtat caaaggcttt cagtgccgga
     2161 agctgttcaa tacctaacgg taacaaatgt gcgcgctgat cgttacccag tagtttacgc
     2221 accgcttcaa actggcacag aaacagttgc gtagggtcga tccccaccgc gaggtgcgcg
     2281 cctgcgccaa tcatgcgcca catgtgataa ccgctgccgc agccgacatc cagaatggtg
     2341 cgtccggtta aatcagaaag atggggcaga acacgatccc atttccagtc ggaacgccat
     2401 tcggtatcga tgttgacgcc atacagtgag aacggccctt tgcgccatgg catcaggttc
     2461 cgcataagcg tttcaatgcg tttaatttgc ccggtgctca gtggctcttc gctttcggcg
     2521 gttacgctat gcaataaatc cagacgatac ggtttaattt caggcagaaa ttccaccgcg
     2581 ttggaccact gcttaaacag cccgtgctgc tgctcgcgct gccagttagc aatctgcgcg
     2641 ggcagcgttt cgagccagtg tgaaagatga tttttggcaa tcagagaata aaagttacca
     2701 aagtcgatca tgcagcgtcc tctgctttta atgccaccag tgaaccaaag ttaaagcact
     2761 ggaaccacag ctcgctatgc tcaaaaccgg ctttatgcag gcgtgcttta tgggtttcca
     2821 cggaatcggt cagcatcacg ttttccagca tgctgcgctt ctggctgatc tccagttcgc
     2881 tgtaaccgtt ggcacgttta aagtcgtggt gcatgttgaa caacagttca ccaactttgg
     2941 catcttcgaa actgaatttt tccgaaagca ccagcgcgcc gcccgggttc agtccttgat
     3001 aaattttatc cagtaatgcc tggcgctcgg aaggttccag gaattgcagg gtaaaattca
     3061 gcaccaccat cgatgcgttt tcaatggcga tatcgcgaat atcaccttca atgacgtcta
     3121 ctggcgtagg ggctttatag gcgtcaatat gacgacggca gcgttcaatc atcgccgggg
     3181 agttgtcgat ggcaataatt ttgcaattat catgatgaat gttgcgacgc accgagagcg
     3241 tcgccgcgcc cagagaacaa cccagatcgt aaacctgcgt accaggttga acgaagcgct
     3301 cggctaacat actaatcatg gaaataatat tggaatagcc gggaacggaa cgctggatca
     3361 tatccgggaa gacttcagct acccgttcat caaaggtcca gtcgcccagt ctggcgatag
     3421 gggcagaaaa tagcgtgtcg cggtgagaca taacgtaaaa atccgggaaa aagaaagtgg
     3481 cgtattgtgc gctaacgcag ggagaaaacc aactcccagg gcatatacca aagattcgcc
     3541 agcaccatca gcaacagcgc acaccaggtg gcgctcatgc cggaacgtcg ccagcgaaac
     3601 agacggtgat gaaaaccgta ataatgcatc agacgaccag caagcaaaac gatgccgcaa
     3661 atatgcacca tccaggtttc tgcgccattc atttccataa acagcatcag cacaatcgcg
     3721 atgggaatat attccaccgc gttaccatga atgcgaatag cgctttgcag ttcgctaaaa
     3781 ccaccgtcac cataggcaac gcggtactgc aaacgca
//
LOCUS       DADLTD010000274         3806 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLTD010000274 DADLTD010000000
VERSION     DADLTD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.278"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..314
                     /locus_tag="JN701_003890"
     CDS             <1..314
                     /locus_tag="JN701_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366164.1"
                     /translation="GSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGL
                     GMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGA
                     H"
     gene            complement(378..893)
                     /gene="luxS"
                     /locus_tag="JN701_003891"
     CDS             complement(378..893)
                     /gene="luxS"
                     /locus_tag="JN701_003891"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY7366165.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(1043..2599)
                     /gene="gshA"
                     /locus_tag="JN701_003892"
     CDS             complement(1043..2599)
                     /gene="gshA"
                     /locus_tag="JN701_003892"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7366166.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(2672..3100)
                     /locus_tag="JN701_003893"
     CDS             complement(2672..3100)
                     /locus_tag="JN701_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7366167.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(3097..3663)
                     /gene="yqaB"
                     /locus_tag="JN701_003894"
     CDS             complement(3097..3663)
                     /gene="yqaB"
                     /locus_tag="JN701_003894"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY7366168.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLAPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT          955 a         1017 c          895 g          939 t
ORIGIN      
        1 cggggtctat cggcacgtcg ataaccacga ccatgtggac caaccgcgag tcgatgcacc
       61 atgcgcagtt gactgagtcg gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc
      121 aactggaagg gcttgggatg acgcaacagc aggcatcagg ctggattgcc cagcagatca
      181 ctaatcaggg gctgattatt tccgccaatg agatcttctg gatgtcagcc gggatattcc
      241 tcgtcctgct ggggctggtg tggtttgcta aaccgccctt tggcgcaggt ggcggcggag
      301 gtggtgcgca ctaagtacaa ctaagccagt tcatttgaac tggctttttt caattaattg
      361 tgaagatagt ttactgacta aatgtgcagt tcctgcaact tctctttcgg cagtgccagt
      421 tcttcgttgc tgttgatgcg tacgtcacgt tccagaatgc tacgcgcaat atcctgcgct
      481 tcctgcaacg agtgcatctg gtaagtgcca cactggtaga cgttcagctc cgggatctga
      541 ttctgatcct gcactttcag cacgtcttcc attgccgctt tccaggcatc agcaacacgc
      601 tgctcatctg gcgtaccaat cagactcata taaaaaccgg tgcggcagcc cattggcgag
      661 atatcgataa tctctacacc attaccgtta agatggttac gcataaaacc agcaaacagg
      721 tgctccagag tatggctccc tctttctggc atcacttctt tgttcggcac gcagaagcgc
      781 agatcgaaca cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc cacccgaact
      841 gcaggcgctt ccatccgggt atgatcgact gtgaagctat ctaacaacgg catttagcca
      901 cctccggtaa tttttttaaa aattttctga actctttctt cccaggcgag tctgagtata
      961 tgaaagacgc gcatttgtta tcatcatccc tgaattcaga gatgaaattt tggccactca
     1021 cgagtggcct ttttcttttc tgtcaggcgt gtttttccag ccacaccgca aacggttcgg
     1081 tatcagcggt ttccatttcc tgctgacggc gttcagatgc ctcgcgctcg gctacaaaat
     1141 cctcttcgcg cagaatttcc aacggctctt cacgcagcag attacggtag gcttctgcaa
     1201 acgctttgcc tgttccgcca ataccagtat caatcataga ccttaagata cgggcagaga
     1261 aagtcagatc gggattatcg aagcaggcga ccagttcatc acacactttc tgatacgctt
     1321 tgccgccgtt aatgctatcc aacgtttgcg cgacgcgttt cagatcgcgg aacagatctt
     1381 tacccacctg cggtaacggg aactgcgcgg tttcgcagcc gatacccagc gtcaggcccg
     1441 gtttgcgacc ttcgaggatc acccggttcc agttaacgcg tgtacaggca agttcgctac
     1501 tgctcatttc cggtgcatca gccagcgcac accagaccat aaacaggtcg aggaatcgca
     1561 cctgctgttc atctacacca atcggcgaga acgggttgat gtccagcgaa cgcacttcaa
     1621 tatattcaat gccgccacgt aacagcgcat cagaaggtga ctcgccgctg cgggtaacgc
     1681 gttttggacg aatcggcgca tacagttcgt tttcaatctg cagcacgttg ctattgattt
     1741 gcagcctctt accgtctttc tcaataccaa tcttcgcgta ctcttccgat ggcgttttga
     1801 ttgcctgttt aaggcccgct acgtactcgt aaagatcgtt gaaggtaata ccaagattgc
     1861 tttgcgattt attggtatag ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt
     1921 aatacatacc gcactcggtt ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag
     1981 aagaacaaat cgccggagat gcaccaaaca gataaggaat gacccaaccg aaacgatagt
     2041 aattgcggat aacgcggaaa tagcccgcag aaattttctc tttggcatca gtgcccgaga
     2101 tatcaccgca cttcgcttgc cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg
     2161 aaatggtttg catcagcgcg ccgtagcgat ttttcagccc ttcacgatac agcgttttaa
     2221 agcgtccggt gttagaagtg ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt
     2281 agcatggcat acttaacggc cacatccgct catcgcccat attgcgcgcc gtataacgat
     2341 gcagatcgcg cataaaggtc aacatatgtt caatatcacc atccactggt gtaatgaatt
     2401 ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg
     2461 cttcaggatg acctgttgtt gcctgtgtgc catcagcatt aacacgcaaa gtttcgcgct
     2521 ccagcccacg ctgtatcccc tttaacgcct gaggatgttt ttccagccag gccagcgcct
     2581 gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa ttagcataac
     2641 tgtaatggtg accatatgtg caggcctaca attagtgcca ccacatcatg ccctgaacgg
     2701 tagctgctgc aactgcaaca tagcgtaaag ctttaccaag gcacaaaaaa aagattaccg
     2761 gtccccacga gatgcgcatc catcccgcta acagacacag taaatcgcca accacgggca
     2821 tccagcttaa taatagcgtg actgcaccat agcgtttcag ccagccagta gctttctctt
     2881 gccagcgcga tgttttacgc aatggaaaga aacgcccaag gataacgtta gttaaccctc
     2941 caaggctatt acccattgtt gctgttaaaa ctaaaaccca gggataactg atcccggaaa
     3001 gcaacattgc caccagcacg acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac
     3061 tactggcaaa caacgagaaa agcgataacg cttcactcac agcaagcgaa catccacggc
     3121 gtccatgcct gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa agaccacaca
     3181 ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt
     3241 atggtgtttg acgtgatcag cagcgacgac ggcgtcaaaa taacggcgta atcccaggtg
     3301 cgccagcaat gcctcagcga tggcgctttc actccccgtt cctacagcca ttgggcgacg
     3361 accatgccaa cttttcacca cttcaacaag aggaagcggc tcgacactat ccagcagcat
     3421 acttcttact gcttctgttt tttcacgcgc taacgcatgc ggggcgagat cggcctgatt
     3481 cagctcaata attgcctgag caatacgcca ggtgggcgat ccattaagcg caatcattgc
     3541 ctgaacatcg tactgaagac cgtagtgccc taatacttcg cgccacgctt tacggtgcgt
     3601 aggctccgta tccaggattg tgccatccat atcaaaaatt aaacctgcat aacgctcgta
     3661 catggtcttc tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc ttgattttgt
     3721 cgctgatggc gaaggagaag aaaattcagg aaatggataa agtagttatt tagaaatgag
     3781 atatttttga aggaaatttt tgggag
//
LOCUS       DADLTD010000275         3780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLTD010000275 DADLTD010000000
VERSION     DADLTD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.279"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN701_003895"
     CDS             complement(<1..209)
                     /locus_tag="JN701_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY7366169.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAK"
     gene            complement(350..2383)
                     /gene="metG"
                     /locus_tag="JN701_003896"
     CDS             complement(350..2383)
                     /gene="metG"
                     /locus_tag="JN701_003896"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY7366170.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQESRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2515..3624
                     /gene="apbC"
                     /locus_tag="JN701_003897"
     CDS             2515..3624
                     /gene="apbC"
                     /locus_tag="JN701_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY7366171.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHCLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          904 a          969 c          971 g          936 t
ORIGIN      
        1 tccttcgctt gctccacata ccccttcttc accttctccg caatcagctt aagctccgct
       61 tttgccgctg ccgcagcatc tgaaaaactt tttatctggc tttgcccttt ggtgccaact
      121 tttccccaac tgatatgcaa ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat
      181 ttttcgtcct gatagataaa gtgtctcatt ctgcatccct gtaattttat ccggttgaat
      241 gcagatgcta ccagtattta tgcgggttag agagagacaa aatcgcaaga aaacaacttt
      301 aggaatttat aaagaaaagg cgctgtcgat gcagcgcctt gaagggggat tatttcacct
      361 gatgacccgg tttagcaccg gcatccgggc ttagcaggaa aatatctttc ccgccaggac
      421 cggcagccat caccatgcct tcagagatac cgaagcgcat tttacgtggt gctaggttag
      481 ccaccataat ggtgtgacga ccaatcagtg cctgcggatc cgggtaagca gaacgaatac
      541 cggagaagac attgcgtttt tcaccgccga gatccagcgt caggcgcagc agtttgtcag
      601 aaccttcaac aaactctgcg ttttcaatca gcgccacgcg caggtcaact ttagcgaagt
      661 cgtcaaaggt gatggtttcc tgaatcggat catctgccag cgggccagtt accggcgcgg
      721 cagcggcttt tacttcttct ttagaggctt ccaccagtgc ttcaacctgc ctcatatcga
      781 tgcggttata cagcgccttg aacggattca ctttgtggcc cagcagcggt tgctggatac
      841 catcccaggt cagttccgta ttgaggaatg cttctgcacg ctcggtcagt ttcggcagta
      901 ccggcttcag gtaagtcatc agcacgcgga acaggttgat gcccattgag caaattgcct
      961 gcaggtcggc atcgcggctt tcctgtttcg ccaccaccca cggagcctgt tcatcgacat
     1021 agcggttagc cagatcagcc agcgccatga tttcgcgcac ggctttacca aattcacggc
     1081 tttcccacgc ttcaccaatc acttcagcgg catcagtgaa ggttttgtac aactgcgggt
     1141 cagccagttc gcttgccagc acgccgtcaa aacgcttgtt gataaagccc gcattacggg
     1201 aggccaggtt aaccactttg ttaacgatat cggcattcac acgctgaacg aaatcttcca
     1261 ggttgagatc gatatcatcg atgcgcgaag agagtttcgc agtgtagtag taacgcaggc
     1321 tgtctgcgtc aaaatgattc agccaggtgc tggctttaat aaaggtgccg cgagacttgg
     1381 acatcttcgc gccgttcacc gtcacatagc catgaacaaa caggttggtc ggcttgcgga
     1441 agttgctgcc ttccagcatg gcaggccaga acaggctgtg gaagtaaacg atatctttac
     1501 cgatgaagtg gtacagctcg gcggtggagt ctttcttcca gtattcatcg aagcttacgc
     1561 tgtcgccgcg cttgtcgcac agattcttga aagaacccat gtagccaatc ggtgcgtcca
     1621 gccagacgta gaaatatttg cccggcgcgt tcggaatttc aaaaccgaag taaggtgcgt
     1681 cgcgggagat atcccactgt tgcaggccag attcaaacca ctcctgcatt ttgtttgcca
     1741 cctgctcctg caacgcaccg ctgcgggtcc atgcctgcaa catttcgctg aaagagggca
     1801 gatcaaagaa gaagtgttca gaatcacgca ttaccggggt agcgccagaa accaccgatt
     1861 tcggctcgat cagttcagtc gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt
     1921 attgatccgg ggatttacat ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca
     1981 tgcctttttc cggatcgtac agctgagaga tggtgcggtt tttaataaaa ccgttttctt
     2041 tcaggcgaga gtagataagt tctgacaact ggcggttctc ttcgctgtgt gtcgagtgat
     2101 agttgtcata gctgatgtta aagcctgcga aatcagtctg atgctcctga ctcatttcgc
     2161 caatcatctg ctccggggtg ataccaagct gctgagcttt cagcatgatc ggtgtaccgt
     2221 gggcatcgtc ggcgcagatg aagttgacct cgtggccgcg cattcgctgg taacggaccc
     2281 agacatcagc ctggatgtgc tccagcatat ggccgaggtg gattgagccg ttagcgtacg
     2341 gcagtgcgca cgtcaccaga attttcttcg cgacttgagt catagtaggc attacttctt
     2401 aatagtgaaa aggggttaga tagtaccaaa tgggaaaatg ttaagtaagt acttctctat
     2461 attcttcagg taaactatca gctgatggtt tatctataca aaaaaggagt cgggatgaac
     2521 gaacaatccc aggccaaatc accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc
     2581 aattttcagc acccaaccct gaagcataac ctcaccacgc tgaaagcgtt gcaccatgtc
     2641 gcctggatgg acgacacgtt gcatgtggaa ctggttatgc cattcgtctg gcatagcgcg
     2701 tttgaagagt taaaagagca atgtagcgcc gaactgctgc gtatcaccgg ggcgaaagct
     2761 atcgactgga aactgtcgca caatatcgcc accttaaaac gcgtgaagaa tcagccgggt
     2821 attaatggtg tgaagaacat cattgcggtc agttccggta aaggcggcgt gggtaaatcc
     2881 tccacggcgg taaacctggc gctggcactg gcggcggaag gggcgaaagt cggcattctg
     2941 gacgccgata tctacggccc atcaattccc accatgctgg gcgcagaaaa tcagcgtcca
     3001 acttcgccgg acggtaccca tatggcaccg attatgtctc attgcctggc aaccaattca
     3061 attggttatc tggtcactga cgacaatgcg atggtgtggc gtggaccgat ggcgagcaag
     3121 gcgttaatgc agatgttgca ggaaaccttg tggccggatc tcgactatct ggtgcttgat
     3181 atgccgccag gcaccggtga cattcaattg acgctggcgc aaaacattcc agtaactggt
     3241 gcagtcgtgg tcaccacccc gcaggacatc gcgttgatcg atgcgaaaaa aggcattgtg
     3301 atgttcgaga aagtcgaggt tccggtgctt ggtattgtcg agaacatgag tgtgcatatt
     3361 tgcagtaact gtggtcacca tgaaccgatt ttcggtaccg gtggtgcaga gaaactggct
     3421 gaaaaatacc acacacagct gctggggcag atgccgttac acatctcctt acgtgaggat
     3481 ctcgataaag gcacgccaac ggttatcagc cgtccggaga gcgaatttac cgctatctac
     3541 cgccagctgg ctgaccgcgt tgcagctcag ctctactggc agggtgaagt cattccaggc
     3601 gagatttcct tccgcgcggt ctaacgccta ttaaccattc tggttattct tcatacaccg
     3661 cagagatgtt acattgatgc ggtgtattag tttttgccgc attaatgaac acttatttaa
     3721 tactgcgaat attctgcgga ccttcgtccg aaactctaca aagtcgattg atcatacagg
//
LOCUS       DADLTD010000276         3738 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLTD010000276 DADLTD010000000
VERSION     DADLTD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.280"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..860)
                     /locus_tag="JN701_003898"
     CDS             complement(<1..860)
                     /locus_tag="JN701_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7366172.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIGMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAE"
     gene            complement(885..1763)
                     /gene="yihU"
                     /locus_tag="JN701_003899"
     CDS             complement(885..1763)
                     /gene="yihU"
                     /locus_tag="JN701_003899"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY7366173.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1936..2832
                     /locus_tag="JN701_003900"
     CDS             1936..2832
                     /locus_tag="JN701_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7366174.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGA
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2872..3660
                     /locus_tag="JN701_003901"
     CDS             2872..3660
                     /locus_tag="JN701_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7366175.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          963 a          956 c          902 g          917 t
ORIGIN      
        1 atttctgcca accgttgtaa acgaggaaca gagacatcgc gcagcatagt ttgtggatct
       61 tgtgcaccaa caaccgacgc ccaaaccgct cgtccagcca ggaagcccga cgcaccacct
      121 ttcatgccaa tacttactgc acggccaaaa atatcggcat caacgccaga agaaagaatt
      181 acccacggca tcttcatctg atcgttaagt tgctgacagg cggcaagtaa ggttttttca
      241 tcgcctttgc cccctaatgg catctcggct ttgtaaagat cagcctctgt tccaccaagc
      301 tctgcggcgg cggcaacaat ggcgctttca cgatcaaagt cccaaccgcg acgaggtgga
      361 cgcaccacag gttcaataat gctcaccagc ccggcactac ggcagcgacg aacaaattta
      421 tcaaccattg ccagacgttc ctctgcgggt tcatcttcac gccagagtac aagtaatttc
      481 attgccttag cccccatttc acgggcttta tgcgggtcga cggacatatc tatttcaacg
      541 ctatcaaccg ggatgccatt acctgggata aaaagatcgg cagcgacaat caggccgcaa
      601 gaattagcaa cagcgccctg ctcgactatt tgctcaagac aaaattgttt atccgccaga
      661 acggcagaag cataaggaga aaggattcgg gtcgcggcaa ctttaaaatc ggtgagtaca
      721 ctgtcggcga tgggttttgg ctgccctgct gccgcaaaca tcaagcgcat ggcctcacgc
      781 tgatcgacag ctaacatggc aaatccacca cctgggcggg agatatcttt cagcgtaaat
      841 ggggtgaagt gggatgacat agaaactcct gttaatagat aaaattaaag accagcctgc
      901 tggcgaacct gatttagcag ggcactccag tcctgccgcc ctttgccgtc ttgacttgct
      961 gcagcgtaat aactttctgc tgcaatcccc atattgagcg gtgtccccgt ctcccgagcg
     1021 acgtcaactg caatacgcag atctttcaac gccagatcca ccatgaaggc gggtgaaaga
     1081 tcgcctttca gcactttgcc aggccaggtg gtcgtaaaat gtcctttccc tgctgccgta
     1141 ccactcatca ccttaattgc gacatcaaga tttaatccca gtgactcaca taacacagcg
     1201 gcctcggagg acagcgcatt aagcgcaatg ctcatatagt tgttaattag cttcacgcgg
     1261 atccccattc ctgggccgcc agcctcgacc agctcattac ccatgcacat aagaatcggg
     1321 cgtgcacgtg caatctgctc atcagtgcca cccgctaaaa tcagcaatgt gccatcaatc
     1381 gcattcacgg acgttctgcc cacgggagcg tccatcatac tgatgccttt ttcttgtaac
     1441 tcccgtatta atgcatcggt ctgtaaggga tgaatagtcg acatatcaat caataacgcg
     1501 tcagatgaaa cagcctcaca gacgcctttt tcccccaaca gaacctgtcg aacaatgtca
     1561 ccattgggaa gcatagtgat gataaattca gcgcctgctg ctgcttccgc ttgggtttgt
     1621 gccgcagtgg ccccctgcgt aactagcgca tcgacggcct gtgcattgac atcaaatacc
     1681 tgcaatgaat gccctttttg caatagattg ttagccattg ggctacccat ctgccccagc
     1741 ccgagaaatg caatggttgc catgaatact ctcctgtctg acattgacaa aataatgaca
     1801 attgaaaatt cgtttttgtc tgtttttgag catatttgtg atttatgttc aaaaacacga
     1861 cacgcatcac taatttgatc atttgcgtca tttaaattga ccaaagacga caaaattaaa
     1921 caggacaaga caaatatgac cagaattgca tgcgtgggta tcacagttca ggacagaatt
     1981 tattctttgc ccactctgcc ggagggcggc gggaaatatc aggcaaatca ttatcttgag
     2041 attggtggcg gcccggcagc aacagccgct gttgcaatcg caaagcttgg cgtggaggtt
     2101 gatttcatcg gtcgcgtcgg cgatgacagt tgtggaaata ccttacttgc agaacttgag
     2161 ggttggggcg tcaataccgc attttgccgc cgatatccta acgcccgttc gtcgcaatca
     2221 gcgatcctcg ttgatcagca tggcgaacgg attatcgtta attatccaag tcctgatctc
     2281 ggtactgatg ccgaatggtt agaagcgatc gatttctctc gttataatct gattctggcc
     2341 gatgttcgct ggcatagcgg tactgaaaaa gcattctcgc tagcgcgcct tgcgggtgtg
     2401 acaacgcttt tagacgccga tatgacaccg caggatattt ctccacttgt cgcgcttgcc
     2461 gatcacgccg tgttctcaac accagggctg aaacgcatga ctggcctgca gtctcctgaa
     2521 gaaggtttgt tccaggcaac gacacaaaca gcaggtaaag tgtatgttac gctgggttct
     2581 gaaggagcgt tgtggattga agatggtcat ttgtgtcaac aggaggcatt ttctgtcaat
     2641 gtggttgata ccactggagc aggcgatgtt tttcacggcg cactcgcggt agcgttggca
     2701 gaaaaaatgc caacaaaaga ggctattcgt tttgccagtg cggttgccgc gatgaaatgt
     2761 acccagcctg gcggtcgggc cggcattcct aatcgtgagc aaaccgaatc atttttgtca
     2821 ctttatgcgt aaaatggtaa acatctaccg aggaggggca agggagtatt catgagtatc
     2881 atcgaagtga caggtaatcc acgccatgac caactcgttc atctaatcgc agaacgcggt
     2941 tatatgaata ttgaagaact ggcacaactg ctggatgttt caacgcaaac cgtgcgccgg
     3001 gatatccgaa aactcagtga gcagggcttg atcacccgtc atcatggagg ggccgggcgc
     3061 gtctccagcg tcatgaatac cgcttttgag caacgggaac tttcgctcac cgccgagaaa
     3121 cgggcgatcg ctgaggcagt cgccgattac cttcccgaac gctgtaccgt ctttatcacc
     3181 atcggtacaa ccgtagaagc cgttgccagg gcattactca accggcgtga tttacgcatt
     3241 atcactaaca gcctacgtgt ggcacagatt ctttataaga atcaggatat tgaagtgatg
     3301 gtgccgggag gcactttacg cgctcataac ggcgggatta tcggcccagg agccgtggat
     3361 tttattgaag gcttccgcgc agattattta atcaccagta taggtgccat tgaacatgac
     3421 ggcaccctac tggaatttga tctaaatgaa gcgttagtcg cgagaacgat gattaaacat
     3481 gcgcggaata cattgttagt ggccgatcat acgaagtttg ccgcgtctgc tgccgtttca
     3541 attggcaatg cacggaatgt cagggctttt tttactgatg ccccgcctcc caattctttc
     3601 tgccagttgt taagtgaaga gaatgttgaa ctggtggttg ccgagcaaga agtatcctga
     3661 aattgaaatt tcggaaaatc cgctgggaat ttcagcggat tttgttttcc ataagcaaaa
     3721 aaaaacctgc cagcgatg
//
LOCUS       DADLTD010000277         3726 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.281, whole genome shotgun sequence.
ACCESSION   DADLTD010000277 DADLTD010000000
VERSION     DADLTD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.281"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(118..2187)
                     /gene="glyS"
                     /locus_tag="JN701_003902"
     CDS             complement(118..2187)
                     /gene="glyS"
                     /locus_tag="JN701_003902"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY7366176.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="JN701_003903"
     CDS             complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="JN701_003903"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY7366177.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3203..3502)
                     /locus_tag="JN701_003904"
     CDS             complement(3203..3502)
                     /locus_tag="JN701_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366178.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
BASE COUNT          862 a         1006 c          959 g          899 t
ORIGIN      
        1 cataaaatta atatatgaga taatctttat tcagcagaag attattaaag gttgctgtat
       61 tatttagcga taataaaaaa agcctgccag atggcaggct ttttaataac ggcgttatta
      121 ctgcaacagc gaaatatccg caacgcgcag gaacagttcg cgcagtttct ccagcatggt
      181 cagacggttg agacgcaatt ctttgtcatc aaccatgacc atcactttat cgaagaaagc
      241 atcaaccggc tcacgcagct ctgccagttc gaccagcgca tcctggtaac gaccttccgc
      301 aaagtacggc tccagcttgt cacgtagcac cacaacctgc atcgccagtt taatttcttc
      361 cggctctttc agggtagagg cattcacgcg gtcgctcagc acttcgtcag atttcgccag
      421 aatgttagat acacgtttgt tcgccgccgc cagtgcagca gctgcatcca gggtacggaa
      481 atgcgatacc gctttcatac gggcatcgaa atcagccgga cgagtcggac gacgcgccag
      541 taccgcctgg atggtgtcaa cggtgtaacc ttcgtcctga taccaggcgc ggaagcgacc
      601 gagcataaag tcgataacat catcaaccac gttggcgtta gtcagcttat cgccatacag
      661 acgcactgct tcttcggtca gcgtttgcag atcaaggttg aggttcttct caacgataat
      721 tcgcagcacg ccaagtgcgg cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg
      781 ctgaccgata ccgaagatac ccgccagagt atccatctta tcagcaatcg ccagcgcaca
      841 agctaccggg ttggacggca ggtcatcacc ggcaaagcgc ggctgatact gctcattcag
      901 cgcgacggct acgtcttccg cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc
      961 ttgggtgtcg gtgaactcga agaccatgtt ggtcatcagg tcacacttag acagcaggcc
     1021 cgcacgggtt gcgtggttaa cgtcagcgcc aatctgttca gcaatccagc cagccagcgc
     1081 ctggatgcgg tcagttttgt cacgcagcgt acccagctgt tgctggaaca gcacggtttg
     1141 caggcgcggc aggttatctt ccagacgttt tttacggtcg gtattgaaga agaactcggc
     1201 atccgccaga cgtggacgaa cgactttctc gttaccagag ataatttgct gcggatcttt
     1261 cgattcgatg ttggcaacga agatgaagtt cggcagcagt ttgccatcgt tcgcatacac
     1321 cgggaagtat ttctggtcac ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag
     1381 gaatttctct tcgaattttg cggtcagaac gaccggccac tccaccagcg aagccacttc
     1441 ttccagcagg ctttcgctta agtcagcgtt accgccaatc ttacgcgctg cttcttcggc
     1501 atcggcttta atcttcgctt tacgttcttc gtaatcggcg atgactttcc cgcgctcacg
     1561 cagaatttcc ggatactgat cggcgttgtc gatggtgaat tccggttcgc ccataaagcg
     1621 gtggccgcga atcacgcgat cggactgaat gcccagaata gttgccggaa tgactttgtc
     1681 gcccagcagc agggtcacgg tgtgcaccgg acgcacgaag tgcacgtcgc ttgcgcccca
     1741 acgcatcagt ttcgggatcg gcagtttcgc cagagaagtc gcaaccatat tcggcagcag
     1801 tgcttcggtg ctttcgccct tcacatgggc gcgatacagc agccattcgc ctttatcggt
     1861 agtcagacgc tcagcctggt caacggtaat accgcaacca cgcgcccaac cttctgccgc
     1921 tttgctcggt ttgccttcag cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat
     1981 ttcacgatcc ggttgcgctt ccgccaggtt agctactttc agcgccagac gacgcggagc
     2041 agcaaaccat tgaacggtgc cgtgtgcgag gccagcgtta tccagctcca cagtaaaatt
     2101 cgcagcaaag gactcaacca ggctgcgcag tgcttttggt ggcagctctt cagtgccgat
     2161 ttccaccaga aaagttttct cagacatagc cgcctcttac ttatctttgt tgcacatcgg
     2221 gaagccgagg gcttcacggg aagcgtagta tgcttctgcc actgctttgg tcagggtgcg
     2281 aatgcgcaga atgtagcgct gacgctcggt gacggagatg gctttacgcg catccagcag
     2341 gttgaagctg tgggcggctt tcagaatacg ctcgtaggct ggcagcggca gcggattttc
     2401 cagcgccagc agctgctgcg cttctttctc gtactgctca aagcaggtga acaggaagtc
     2461 cacatccgcg tattcgaagt tgtaagtgga ctgctccact tcgttctgat ggaacacgtc
     2521 gccgtaggtg gttttaccca gcgggccgtc gctccagacc aggtcgtaaa cgctgtctac
     2581 gccctgaatg tacatggcca gacgttccag accgtaggtg atctcgccgg taaccggttt
     2641 acactccaga ccaccaacct gctggaagta agtgaactgc gtcacttcca tgccgtttag
     2701 ccacacttcc cagcccagtc cccaggcacc cagagtcggg ttttcccagt tatcttccac
     2761 gaaacggatg tcgtgaatag tcgggtccat gcccagctct ttcagagaac cgaggtacag
     2821 ctcctgaata ttgtccggcg atggcttaat gaccacctgg aactgatagt agtgctgtaa
     2881 acggttgggg ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct gaacataagc
     2941 agccgccatc ggttctggcc ccagcgcgcg cagacaggtc attgggtgag aggttcccgc
     3001 gccgacttcc atgtccaatg gttgaacaat ggtgcagccc tggcgagccc agtaatcctg
     3061 taaggtcagg atcaagccct ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc
     3121 tggatacgtg tggatttaaa ggaagggatc agtatacccg ctggatggaa gatatacagt
     3181 acgaaacggg aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt
     3241 caaaagaaca gacaaactgc tctttacgga aggtataacc gcgcatttca tagcttccct
     3301 ggaactgttc gaaggcggtg acgtcgattt tttgctcgcc ggtgttataa cgttttgccg
     3361 cctgatcctt gcataacgct tccatattca acgaacgcag aggatcaacc tttacccgtt
     3421 gtgctttctg cacgggtgac ggtgtagaac aacccactag caccataagc gccattgccg
     3481 gaaagaaagc gttcatcatc attaaattac cgtctggttt gcaggtgagt cttattattt
     3541 atatcggtat agtggctgat atcttacgaa tttccttaag cctctataag ctgtagtgat
     3601 aaaactcaga ttattccagt gcgccaacca aatgatttat cggtcagact ttttttaacc
     3661 agatacagag acacagatgc aggtaatgct gccaacttac tgatttagtg tatgatggtg
     3721 atttta
//
LOCUS       DADLTD010000278         3723 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLTD010000278 DADLTD010000000
VERSION     DADLTD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.282"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            37..264
                     /locus_tag="JN701_003905"
     CDS             37..264
                     /locus_tag="JN701_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7366179.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(265..639)
                     /locus_tag="JN701_003906"
     CDS             complement(265..639)
                     /locus_tag="JN701_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7366180.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="JN701_003907"
                     /pseudo
     CDS             complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="JN701_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2118..3038)
                     /locus_tag="JN701_003908"
     CDS             complement(2118..3038)
                     /locus_tag="JN701_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY7366181.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3263..3723
                     /locus_tag="JN701_003909"
                     /pseudo
     CDS             3263..3723
                     /locus_tag="JN701_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
BASE COUNT          916 a          952 c          944 g          911 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc attattagtc gcactatacc ggggcagggg
      121 catggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ctggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 attcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg ctgcgcgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcgtatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg cccggcgatc tggttgctcg agttgtaaac caacaatgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgct gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga ccggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggtggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ccaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctga agacaatacc ggaccatatt cagggcggag
     1801 tgaccggtaa cgccaagctg ctcaacgtag aggggtaaga agggcattac cagactgaag
     1861 gcggcaccgg taagaaaaca gcctagccag gcgacgatca ggtttcgttt ccagtttata
     1921 ggggtgtcat tttcacaggg tgacatagca atccgctgtt ggtgcgccag gcgcggtgaa
     1981 cataagaaga aaagataagc acactaatta tgcgcccgac ttccaggggg cgcaatccag
     2041 agagctttta tcgctaaatc agggggattt gctgtggtaa tgccggatgc cattctgaag
     2101 catccggcag gggagattta atagcgtgaa ggaacgcctt ccgggcgtgt tttaaagcga
     2161 cggtgtaacc acatatactg ctctggtgcc atcatgatgc atttttcgac cactttgttc
     2221 atccacgcgg cggtagtttc ggcatcatcc agtggcggag aacactctgg cggcagcata
     2281 atcaattgat accctttgcc atctggctta cggcgtggaa cgaagggcac cagacatgcg
     2341 ccggacatcc gtgccagcat ccaggttccg gtcgtggtcg cagcctgctc aacggcaaat
     2401 aacgggacga aaacgcttga gcgcgggccg taatcatgat ccggtgcgta ccagaccact
     2461 tcgccttttt tcagggcttt aatcatgcct tttaaatctt tgcggtcgag catcgattta
     2521 tttgagcgca aacggcccca ggtttgtagc cagtcaatca gtggattatc gttcgggcgg
     2581 taaacgccaa tccccggttc ctgcatacca aactgccgcg cacccagctc cagtgtcaga
     2641 aaatggatgc caactaacag gatgccgcgt ttttgcgcct gcacgtcacg aatgtgttcc
     2701 atgccgatca cttccgtcca gcgcgcgatt cggcggtccg gccagaacca cgccatgccg
     2761 gtttccatca ggcccatgcc aacggattcg aaattcttca ccaccatttt acggcgttct
     2821 tgttcgctca tttccgggaa gcacagttcc aggttgcgat gcacaatttt tgcgcgtcgt
     2881 ttcataaaac gtaacgccag ttttcctaat ccacaaccga ggcggtagat aaccgggtag
     2941 ggcaattgca cgactaacca aagtacgcca atacccaacc aggttaacca ataacgcgga
     3001 tgaagcagtg cggtggagaa cttgggtaga ttcgtcatat caatcctgtt tttcaaccta
     3061 ttcgggcaat tgtatgtatt gtcgcatttt ttcgcccgca accaaaattt gtggctgaag
     3121 actgggcgaa attgccgcgc ttgtaaataa caaataattt ttaatgcgca aatgtagcgt
     3181 aaaatgtgtg gatgttaatt atcgataatt gctatatcat gccgcggatt tttactttcc
     3241 catctcgcag gaaccgtaca ccatgccagt gttacacaac cgcatttcca acgacgcgct
     3301 aaaagccaaa atgttggctg agagcgaacc gcgaaccacc atttcgtttt acaagtattt
     3361 ccacatcgcc gatcctaagg cgacccgtga cgctttatat cagctgttta ccgcgctgaa
     3421 tgtttttggg cgagtgtatc tggcgcatga gggcattaac gcacaaatca gcgtacctgc
     3481 gagcaatgtt gaaacatttc gcgcgcagct ctgccttcga cccggcttta gagggtttac
     3541 gcctgaatat cgcgttggat gatgacggga aatccttctg ggtactgcgc atgaaggtac
     3601 gtgatcgcat tgttgccgac ggtattgacg atcctcactt tgatgccagc aatgttggtg
     3661 agtatctgca agcggcggaa gtgaacgcca tgcttgacga tcccgatgca ctatttatcg
     3721 aca
//
LOCUS       DADLTD010000279         3697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLTD010000279 DADLTD010000000
VERSION     DADLTD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.283"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(2..382)
                     /gene="panD"
                     /locus_tag="JN701_003910"
     CDS             complement(2..382)
                     /gene="panD"
                     /locus_tag="JN701_003910"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY7366182.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            656..1552
                     /locus_tag="JN701_003911"
     CDS             656..1552
                     /locus_tag="JN701_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7366183.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            complement(1844..2695)
                     /gene="panC"
                     /locus_tag="JN701_003912"
     CDS             complement(1844..2695)
                     /gene="panC"
                     /locus_tag="JN701_003912"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY7366184.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2707..3501)
                     /gene="panB"
                     /locus_tag="JN701_003913"
     CDS             complement(2707..3501)
                     /gene="panB"
                     /locus_tag="JN701_003913"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY7366185.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
BASE COUNT          885 a          928 c          969 g          915 t
ORIGIN      
        1 atcaagcaac ctgtaccgga atcgctttcg cggtacgttt catttcattg tcgccttcaa
       61 aataggcgac gttgggtcgc caggtgcgag cttcttcatc tggcatggta acgaagctgg
      121 cgatgatgac aatatcgccg acactggcgc agtgggccgc cgcaccgtta acagaaataa
      181 ttctcgaacc gcgttctgcc gcgatggcat aagtggagaa acgcttgccg ttggtgacat
      241 tccagatatc aatggcttcg ttttcgagaa taccggctgc gtcaagaaaa tcctggtcaa
      301 tggcgcaaga accttcatag tgcaggtccg catgagtcac tttcacgcgg tggagtttgc
      361 cctgcagcat cgtgcgaatc ataacttcta cctttctacc ctgtcgttaa cgaagcaggc
      421 gatgcctgct ttgaggaaat tctcacgcag tattgcccga tttttgttta gtgtctactc
      481 atctgacggc atttacatca gcagtttgcg taccgtgccg aagcgtgcca tgtaacggct
      541 gtaaccaatt gaaatttctc tgaacgctgc tcggtaaaca ctaaagaggt ggctgacgat
      601 ggcacgcctt gactggatac tgccttcacg caagacaaca cggaaggcag ggaagatgga
      661 tgcaccgagt accacaccgc atgatgcagt gtttaaacaa tttttaatgc acgcggagac
      721 ggctcgcgac tttctggaga tacatttgcc agtggaatta cgagaacttt gtgacctcaa
      781 cacgcttcat ttagagtcgg ggagtttcat tgaagagagc ctgaaaggac acagcacgga
      841 cgtgctctat tccgtgcaaa tgcagggcaa tcccggttat ctgcatgttg tgattgaaca
      901 ccaaggcaag ccggataaga aaatggcctt tcgcatgatg cgttattcta ttgctgccat
      961 gcaccggcat ctggaggcgg atcacgataa gctgccgctg gtggtgccga tactgtttta
     1021 tcagggcgag gccacacctt atccgctctc aatgtgctgg tttgatatat tttactcgcc
     1081 ggagctggca cgacgcgtct ataacagtcc tttcccgctg gtggatatca ccatcacacc
     1141 ggatgacgaa atcatgcaac atcggcggat tgcgattctc gaactactgc aaaaacatat
     1201 tcgccagcgc gacttaatgt tattgctgga gcaactggtc acgctgattg acgaagggta
     1261 cactagcgga agtcagttag ttgccatgca aaactatatg ctgcaacgcg gtcatactga
     1321 acaagcggat ttgttttatg gtgtgctgag agacagggaa acgggagggg agtctatgat
     1381 gacgctggcg cagtggtttg aagagaaagg gattgagaag gggattcagc agggaagaca
     1441 ggaggaaagg caagaattcg cccagcgttt tctgagtaaa gggatgtctc gggaagacgt
     1501 tgcagagatg acaaatttat ctcttgctga gattgatagg ctgattaact aagttgtctg
     1561 cgttatgaat aattgcttac agacgccatc gccatgacgt ggggcaaatt aataattggc
     1621 gccatcagcc gtagcgccag ttatactcgt catactttaa attgcatgtg ctgtgtctgc
     1681 gttcgctaac cccagtcact tacttatgta agctcctggg gattaatgag agacatccat
     1741 gtctctcacc gaaggccagc ctgcggctgg gcaaattcgt tcctgacgaa tttgtcactc
     1801 gcttgccgcc ttcctgcaac ttgaattatt tagagtaaaa gcattacgcc agttcgacga
     1861 ttttgttgtc gatcaggcgg gcatcgccaa gccaggcggc taccagaatt acagctcgtt
     1921 tgctggtttc agagacttcc agcaacgtgt cggcatcgcg aatctgaata tcatcggcgc
     1981 ggaagccttt ttcattcagt tcttgccctg caatggcaat aatttcatca agatcccgct
     2041 cgccagcctg caatttgtcg gcaattgaac ttaaaacttt gtacagaccg ggcgcaattt
     2101 tgcgttgttc cgccgtcaga taaccattac gggaactgag cgcaaggcca tctttggcgc
     2161 gcataatcgg gacgccgaca atttcaatat cgaagcccat atcggcaacc attttgcgga
     2221 ttagcgccag ttgctgaaag tccttttcac cgaagcaggc gatgtccggc tggaccaggt
     2281 taaacagctt gctgacgata gtcgaaacgc cgcgaaaatg ccccggacgg ctcgcgcctt
     2341 ccagcatggt cgaaagcccg ggaacgtcaa cgtaagtatg ggtttcagta ccgttcgggt
     2401 agatctcttt taccgaaggg gcgaaaacta aatccacttt acgtttgttt agcttctcgc
     2461 agtcctcctg caaggtccgt ggataacgtg ccagatcttc cgggcggtcg aactgcatcg
     2521 ggttaacgaa aatactgacg acgaccacat cggcgcgggc tttggcttcg tcgaccagct
     2581 tcatatggcc atcgtgcaga ttacccatag taggcaccag cgccacgcgc ttgccttcca
     2641 tacgcaggcg gcgaatttgc tgacgcagca gcggcagggt ttcgataatt aacacaacgt
     2701 gactccttaa tggaaactgt gttcttcgcc cggataaacg ccggactcca cttcagccat
     2761 atactgccgc acagccgcgc ggatgtcgcc cgtttcggcg aggaaatttt tagcgaattt
     2821 aggaatgtga ccgccggtaa tgccgaaggc gtcgtgcatc acgaggatct gcccgtcagt
     2881 gacgttgcct gcgccaatgc caataaccgg gatcgccagt gcttcggtaa tacgttttgc
     2941 cagttcaacc ggcacgcatt ccagcaccag cagctgtgcc ccagcggctt ctaaggctaa
     3001 tgcatcgctg agcagttgat cgcccgcttc atcgccgcgc ccctgaactt tgtagccacc
     3061 gaaaatattc actgactgtg gggttaaacc taagtgacca catacaggaa cggcacgttc
     3121 ggtcagcatt tttacggttt cgaccagcca ctcaccgccc tcaattttga ccatattggc
     3181 accggcacgc ataaccgttg cggcgttttc aaaggcttgt tccggcgtgg catacgccat
     3241 aaacggcagg tcagccaaca gcaggcagtt tggcgcacca cgacgtacgg aggcagtgtg
     3301 gtaggcgata tcggcaacgg tcaccggcag ggtggagtcg tgaccctgaa ccgtcatgcc
     3361 cagcgagtcg cctaccagca tgacgttaag tccttcatca gcaaatagtt tcgcgaagct
     3421 gtagtcgtaa gcggtgatgg tcgcgaaacg ctttttatcc tgcttacatt tctgaagtga
     3481 agcaatggtg gtcggtttca taacgtgtcc tgataaatga tgtcgtgttg cctggcattt
     3541 tatcagtcac attggtgggg gcaatgattt atcaattacc gcaccgccag atgatggcgg
     3601 tgcggtggac ttattaaggg taggttacct ggaaagtact ggtggcttta aactcaccag
     3661 cttctattgg tatagttcca tcctgttgta gcgtggc
//
LOCUS       DADLTD010000280         3690 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.284, whole genome shotgun sequence.
ACCESSION   DADLTD010000280 DADLTD010000000
VERSION     DADLTD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.284"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..1555
                     /gene="cas3"
                     /locus_tag="JN701_003914"
                     /pseudo
     CDS             1..1555
                     /gene="cas3"
                     /locus_tag="JN701_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
     gene            1653..3215
                     /gene="casA"
                     /locus_tag="JN701_003915"
     CDS             1653..3215
                     /gene="casA"
                     /locus_tag="JN701_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HAY7366186.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRAWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            3212..>3690
                     /gene="casB"
                     /locus_tag="JN701_003916"
     CDS             3212..>3690
                     /gene="casB"
                     /locus_tag="JN701_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HAY7366187.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLEEGVFRWCQESDDLLNHHRRQQRPTELIR"
BASE COUNT          955 a          812 c         1011 g          912 t
ORIGIN      
        1 taatgaatca ctctggtccg gtgatttaat cgggtcagaa gaaccggatg aacaagcttt
       61 cagccagggt tgtgcggcct agtttgccga cagtaacaag aaggcgttgc tggctgaaat
      121 tggcgtcggc accctggatc aggcgatgat ggcagtgatg ccgtttaaac ataataatct
      181 gcggcttctg gggctgagta acaaaatcct gctggctgat gagatccacg cctgtgacgc
      241 ttatatgtcg tgcattcttg aagggctgat cgagcggcag gcgcgtggcg gaaacagcgt
      301 tattttgctt tctgctacgt tatctcaaca gcagcgcgac aaactcgtcg ccgcctttgc
      361 gcgtggtgca gagggccagc aagaagcccc actcctggga aaagatgatt acccctggct
      421 gacgcatgtc acgaaaaccg atgtacattc gcaccgggta gcgacgcgca aagaagttga
      481 acgtagcgtg agcgtgggtt ggctccatag tgaacaagag tgtattgcgc gtatcgaatc
      541 ggcggtaagt caggggaaat gcatcgcctg gattcggaat tctgttgatg acgctattca
      601 ggtgtatcgt cagctgcttg cccgcggcgt cattcctgct tccagccttt cactttttca
      661 tagccgcttt gcttttagcg atcgccagcg aattgaaacg gaaacgctgg cacgctttgg
      721 taaatattgt agtttacaga gagcgtctca agtcattgtt tgtacacaag taattgaaca
      781 aagcgtggat attgacctgg acgaaatgat ctccgacctg gcccccattg atttgctaat
      841 ccagcgagcg gggcgattac agcggcatat ccgcgatatt aatggtcagt taaagcgtga
      901 cggaaaagac gagcgttccc cccctgaatt gctgattctg gcccccgtct gggatgacgc
      961 tcctggtgac gaatggttcg gcagtgccat gcgtaacagt gcatatgtct atcccgatca
     1021 tgggcgaatc tggctgacgc agcgtgtgct gcgtgagcaa ggcgctattc agatgccgca
     1081 cgcagcccgg cttcttattg aatcagttta cggtgaggac gtggtaatgc cggaaggatt
     1141 tgcccgcagc gagcaggagc aagtgggcaa atattactgt gatcgcgcaa gggctaaaaa
     1201 gtatgtgctg aacttcaggc tcggctatgc cgccaatatc aacgattacc ttccggaaaa
     1261 actgtcgaca cgtctggctg aagagtctgt ttccctgtgg ctggctacct gtattgacgg
     1321 tgtggtgaag ccttatgcca ccggtgctca cgcatgggaa atgagcgttg tcagagtgcg
     1381 ccgaagctgg tggaaaaaac atcgggatga gttttcttta ctggaagggg atgcgttcag
     1441 gcagtggtgc gttgaacagc ggcaagatcc ggaaatggca aacgtgattt tagtcaccga
     1501 tgacgaaagt tgcgggtatt cggccaggga gggattgatt ggcaaggttg gttgagatgt
     1561 ctgcgacaag actctctgaa tagttgatat acagaaaagg atggaaggat ggacaaccga
     1621 cctgggatgg tcttttttac gagaggatgc gtatgaactc gttttcactt ctgacaaccc
     1681 cgtggttgcc cgttcgtttt aaagacggaa cgacaggcaa actggcacca gtcgatctgg
     1741 ctgatgaaaa tgttgtcgat atcgctgcgc cgcgggcaga tctccagggg gcggcctggc
     1801 agtttttgct ggggctacta caaaccagct tcgcgccaaa agatcaacgt agttgggatg
     1861 atatctggga agacggactg gaagctgaaa agctaaggga agcattgctg tctttagaac
     1921 atgcttttca gtttgggcca gattcccctt catttatgca ggattttgat gaattaaaag
     1981 ttaaggcaac ttttattgct tcgcttcttc ctgacgcacc aggaaaacaa acaaaagaac
     2041 gaaatacaga ccattttatt aagcgagaca caactcagca tctgtgcctg cattgtgtgc
     2101 ctttagcgct tttttcaatc cagttgaatg cacctatagg tggacgcggt tactatcctg
     2161 ggctacgtgg cggagggcct ctgacgacac ttatagaatt gctggagtat cagggcaatc
     2221 aacaaacccc tttgtggcga aagctgtggc taaacgtaat gccgcaagat gaagccgatt
     2281 tacctttacc aaaaaccttt gatgacctgg tctttccttg gcttgcaccg acgcgtacca
     2341 gcgaactgga cggtgctgtg gtaacggatg aacaagtcaa taaacttcag gcctactggg
     2401 gaatgccacg acgtatacgt attgatttta agacaacctc aataggtaac tgcgacattt
     2461 gcggtaggca gagcgatgct cttttgggct tgatgtcact caaaaactat ggtgttcaat
     2521 acgtcatgtg gcgccatcct ctaacacctt accgtctgcc gcttaaagag ggtggagatt
     2581 tttactcagt taaaccacaa cctggtggtt taatctggcg cgcctggtta ggcctcatag
     2641 aggtaggtaa ctcaaaaaac aatactgaac tccccgcaca ggtcgtgaaa cttttgaatg
     2701 ccagcaatct aaagcagaca agggtgggct tatggggatt tggttttgat tttgaagata
     2761 tgaaggttcg ttgttggtat gaacaccatt tcccgctgtt gttaaacaaa aaagaagacc
     2821 taatacctaa gttgcgtctg gcagcgcaag cggcatcaca cattctaagc ttgttacacc
     2881 gagcgttgaa agaagcatgg ttctctgaga agaaaaccac aaagcttgat ttcggttttg
     2941 tggatattga cttctggaac aaaactcagc atcgattcct gagattagtg cgcaaaattg
     3001 aagaaggtca ggatccggac gaattactca gtaaatggca aaaggaaatg tggttattcg
     3061 cgcgtcagga ttttgacgat cgtgtcttta ccaatccata tgagcccgtt gatttggaac
     3121 gcgtcatgac cgcacgcaag aaatatttta caacatcagc ggagaagcaa aatgctaacg
     3181 ccgccaggga gaaaaagcag gaggctgctg aatgagtatt gtaaaagatg agcataaagt
     3241 tgcgctgcgt aaatggcatg aagagttgca ggataaacgc ggcgagcgtg ccagtctgcg
     3301 gcgtagtacg acggttaatg atgtttgtct gacggatgga tttcgcctgt ttcttaagaa
     3361 cagacaaata aagtggcagg atgaacccga gtggcgtatc accgcactag cgctgatagc
     3421 agcagttagc gccaatgtta aagctattga cgaacggcag ccttttgccg cgcaactggc
     3481 ggcggtaatg aaaaaggggc ggtttactcg tttgttggca gtaaaaacgc cggatgattt
     3541 attgcggcag ttacgccgtg cggtcaaatt attaaacggt tccgtcaatc ttgactctct
     3601 ggaagagggg gtcttccgct ggtgccagga gagcgatgac ctgctcaatc atcaccgccg
     3661 ccagcagcga ccaacagaat taatccgtat
//
LOCUS       DADLTD010000281         3685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLTD010000281 DADLTD010000000
VERSION     DADLTD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.285"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(279..566)
                     /locus_tag="JN701_003917"
     CDS             complement(279..566)
                     /locus_tag="JN701_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366188.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(641..784)
                     /gene="mgrB"
                     /locus_tag="JN701_003918"
     CDS             complement(641..784)
                     /gene="mgrB"
                     /locus_tag="JN701_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY7366189.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            943..1182
                     /locus_tag="JN701_003919"
     CDS             943..1182
                     /locus_tag="JN701_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366190.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1325..2116)
                     /gene="kdgR"
                     /locus_tag="JN701_003920"
     CDS             complement(1325..2116)
                     /gene="kdgR"
                     /locus_tag="JN701_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY7366191.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2293..3666
                     /locus_tag="JN701_003921"
     CDS             2293..3666
                     /locus_tag="JN701_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7366192.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          924 a          905 c          814 g         1042 t
ORIGIN      
        1 taaaataaga aaaattgatg aatgagcaaa aaaatcaaga gagaaacgtt tcgctaataa
       61 ataataccac gcttaatcac aaatccgatg taaacatcct aataaattaa tgaggaaaat
      121 atctttttct gatatcccgt tacgcaaata aataatatga taattatttg aagtcagacc
      181 agagttttaa tttgaagcag ttgcaccatg acagtgcgta taatcaggat gaattgagta
      241 acccttcaac gacggggtta ctcaagattg aggaaggatt atcgttcggc gaccaggcga
      301 ataatatctt cgtcatcaac cgatttttga ctctcaatgg tctttttatc tgctttctct
      361 ggttcgtttg cttcgcacaa acgacgtaac agtgcatttt gacgtttttg ctgatccagc
      421 aacgcctcaa gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac
      481 cacaagatga gcccgataag aagaactacc accgatacaa ccaaagacgc aatgttcatc
      541 acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca
      601 ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt
      661 taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct
      721 gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt
      781 tcactccaat ctccgcttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca
      841 gcgcctaact atgtcgatat tcatgctttt tcggaatgag tagcttgctt cagcgcatat
      901 tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc
      961 acagttatgc tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg
     1021 attggaagca aagagaatgc cgcaggatta ggtttgcaat gtacctatct gactgccaga
     1081 ggcaccagca cggtacaata cttgcacact aaaagtggtt tcttggggat aacagattgc
     1141 ccactgttac gtaaaagcaa tatcgtggtc gataatggtt aagggtaata aataaccaga
     1201 ttctcactca tgagtaaaat attcttatag caagcgcgtt taatataacg caccttcaac
     1261 tttatttttc tcatgctaaa taaagaacct gtctgtttga tattccgaca ggttcttact
     1321 ctcatcagaa cggatagtcg tgataaccca tttgggcaga aattttgcgc gctgcggtat
     1381 gcaacattgc gacatattcc tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga
     1441 tgctcaaacc ggcaatgacc acgccaaagc gatcaaatac cggtaccgca atgcatcgca
     1501 gcccttcttc ctgctcttcg ttatcttcgc catacccctg ctcgcgcacc tggtccagaa
     1561 cgggtaataa cgcttctgta ctggtgatgg tccgctcggt actgcgttta tactccacgc
     1621 cctcaagaat ttgcttcact tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg
     1681 cggtgctgta cagcggatta cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag
     1741 agtcaatttt gtgaatgtaa acaatactgt cttcgtccag tgcgccgagg tggatagttt
     1801 ctttggtcag gcgggagatc tcacgcatct ggatatctgc gctacgaatt aaatcgacgt
     1861 tttgtaacgc gcgagcgccc agttcaaaca atttcagggt cagggaatat ttctccgact
     1921 ccccttcctg cgccacataa cctaaggttt tcatggtctg taaaaagcga taaacggtgc
     1981 tttttgacat catgacgcgc tgcgacagct cggttatccc tatttcgcgc tcttcaccca
     2041 gcgcctgcaa aatgccaaaa acttttagca cggaagatac agaatcaggc tgtttatcca
     2101 gatctgcgtt agccatttat cacctcattg cgagtgtttt ataaaaatca gaactgtttt
     2161 ttattataat ttcgcaccag gggggtcgca atccatcttt tgccggttag ttacaattct
     2221 gcgacatcca ccgtgaatat cagtgctaga atcatacccc tgttgattat tcaccaaaga
     2281 tataaaattc ctatgccaaa agttcaggcc gacggcctgc cattgcccca gcgatacggt
     2341 gcgatattaa ccattgtgat tggtatttcg atggctgtcc ttgacggcgc aatcgccaac
     2401 gtcgccctgc caacaatcgc cacggacctt catgccacgc cagccagttc catctgggta
     2461 gtgaacgcct atcaaatcgc cattgtcatc tccctgctct cgttttcgtt tctgggcgat
     2521 atgtttggct atcgacgtat ttataaatgc ggtctggtcg tttttctgtt gtcttcactg
     2581 ttctgcgccc tttctgattc gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc
     2641 ggcggtgcag cgttgatgag cgttaatacc gcacttatcc gcctgatcta tccacaacgt
     2701 tttctgggga gagggatggg cataaactcg tttattgttg ccgtctcttc tgctgccggg
     2761 ccgacaattg ctgcagcaat cctctccatc gcatcctgga aatggttatt tttaatcaac
     2821 gtaccgttag gtattatcgc cctgcttctg gcgatgcgtt ttctgccacc caatggttct
     2881 cgcgccagta aaccccgttt cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc
     2941 ctgcttatca ctgcgttgag tggtttcgct caggggcaat cgctgacgtt aattgctgcg
     3001 gaactggtgg taatggttgt tgttggtatt ttctttattc gccgccagct ttctcttccc
     3061 gtaccgctgc taccggtgga tttactgcgt atcccgctgt tttcactttc tatttgcaca
     3121 tctgtttgct ctttctgcgc acaaatgctg gcaatggttt ccctgccctt ttacctgcaa
     3181 accgtgctcg ggcgtagtga agtcgaaaca ggtttacttc tgacaccgtg gccgttagca
     3241 acgatggtga tggctccgtt ggcaggctat ttgattgaac gcgtacatgc aggattgctg
     3301 ggtgctctag ggttgttcat catggctgcg gggctttttt ccctggttct gctgcccgcg
     3361 tcacctgcgg atatcaatat tatctggccg atgatcttat gtggcgctgg atttggcttg
     3421 ttccagtcac ccaataacca caccattatt acctccgctc ctcgcgaacg tagcggtgga
     3481 gccagtggca tgttaggaac ggctcgtcta ctgggtcaga gtagcggcgc ggcactggtg
     3541 gcgctgatgc taaatcagtt cggtgataat ggtacgcacg tttcgctgat ggctgcggct
     3601 attctggcag tgattgctgc ctgtgtcagt ggtttacgta tcactcagcc acgatccagg
     3661 gcataaaaaa agcgcgtcga tcagg
//
LOCUS       DADLTD010000282         3676 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLTD010000282 DADLTD010000000
VERSION     DADLTD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.286"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(167..481)
                     /gene="fliE"
                     /locus_tag="JN701_003922"
     CDS             complement(167..481)
                     /gene="fliE"
                     /locus_tag="JN701_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY7366193.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            696..2354
                     /gene="fliF"
                     /locus_tag="JN701_003923"
     CDS             696..2354
                     /gene="fliF"
                     /locus_tag="JN701_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAY7366194.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVNQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2347..3342
                     /gene="fliG"
                     /locus_tag="JN701_003924"
     CDS             2347..3342
                     /gene="fliG"
                     /locus_tag="JN701_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY7366195.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3335..>3676
                     /locus_tag="JN701_003925"
     CDS             3335..>3676
                     /locus_tag="JN701_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY7366196.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAKAKSQQAPIHARMQQLV
                     SEFQTTLDALDS"
BASE COUNT          927 a          966 c         1026 g          757 t
ORIGIN      
        1 ttcctatgag caaagcgaag acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga
       61 aaactttcag gatcaggtat ccattttaaa ccagaaatag ggtgagtttg tcccatccat
      121 gccccacgcg ggcagatggg atgtaattac caacaggtta caaaacctac acctgcatgg
      181 acatcacttc ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag
      241 aaactgaggc tttttgcata tcggtcatca catcgtttaa cgccacgccg ggttcaccga
      301 gggtgaattt ttctgcctgc gtgcgggcgg ctgtttgtgt atcgcttatg cgatcgagcg
      361 cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg cagtgaatcc tgcgcacgcg
      421 cactcatcgc cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca
      481 ttttcgtctc ctggataatt tctggtagca aagcctacca gtaagtcaat aagacaaagg
      541 cgctaaatag caacaaaaaa acgggtttat tggcggatag aaaaaaacga aagcacaaat
      601 aatggcagcg tcaatttttc gagtttgctg acccgggagt gagtcttgtt ccactttgcc
      661 aataacgccg tccataatca gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa
      721 aatctcttga gtggcttaat cgcctgcgtg cgaatccgaa aattccattg attgttgccg
      781 gttccgcggc agtggcggtc atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc
      841 gcacattatt cagcaatctt tccgatcagg atggtggcgc aattgtcaac caactgacgc
      901 aaatgaatat tccttaccgc ttcagcgaag ccagcggcgc tattgaagtt ccggcagata
      961 aagttcacga gctgcgtctg cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg
     1021 gtttcgaact actcgatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt
     1081 atcagcgggc gctggaaggc gagctttctc gtaccatcga aactatcggc ccggtaaaag
     1141 gggcgcgcgt acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc
     1201 cttctgcatc ggtgacggta aatctattac ccggccgcgc actcgatgaa gggcaaatta
     1261 gcgccattgt acatctggtt tccagcgcgg ttgctggtct gccgccgggg aacgtcacgc
     1321 tggtggatca gggtggacat ctgttaaccc tgtccaatac cagcgggcgc gatcttaatg
     1381 acgctcagtt gaaatatgcc agcgatgtcg aaggccggat tcagcggcgt attgaagcga
     1441 tcctgtcgcc aattgttggt aacggtaata ttcacgccca ggtcacggcg caactggact
     1501 tcgccagtaa agaacaaacg gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg
     1561 cgcttcgttc acgccagctt aatgagagcg agcaaagcgg ttccggttat ccgggcggcg
     1621 taccgggggc gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc
     1681 cggcaaatca aaataaccgc cagcagcagg cgagcaccac cagcaatagc gggccgcgta
     1741 gcacacagcg gaatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa
     1801 tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt ggtaaattac aaaaccttgc
     1861 cagacggtaa accattgcct ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc
     1921 gcgaggcgat gggcttttct gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt
     1981 tcaatagcag tgacgaaagc ggcggagaac taccgttctg gcaacagcaa gcgtttatcg
     2041 atcagttgct ggctgccggt cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc
     2101 ggaaagcggt acgcccgcag ttaacaagtc gcgccgaggc gatgaaagct gtacagcaac
     2161 aggcgcaggc ccgtgaggaa gtggaagatg cggtggaagt ccgcctgagc aaagacgaac
     2221 aactccaaca acgacgcgct aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc
     2281 gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata
     2341 acgatcatga gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc
     2401 gaagaccggg cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc
     2461 gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggag
     2521 tttgagcaag aagctgaaca gtttgccgca ctgaatatca acgccaacga ttatctgcgt
     2581 tcggtattgg tcaaagctct gggtgaggaa cgtgccgcca gcctgctgga agatattctc
     2641 gaaactcgcg ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagtgcc
     2701 gccgatctga ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgaag
     2761 cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg
     2821 ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc tggcggaact gaccgaagta
     2881 ctgaatggct tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg
     2941 gcagccgaaa ttatcaacct gatgaaaact cagcaggaag aagccgttat taccgccgtg
     3001 cgcgaattcg acggcgagct ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat
     3061 ctggtggatg tcgacgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg
     3121 ctgttgatcg cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg
     3181 tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg
     3241 tcgcaggtgg aaaacgaaca gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact
     3301 ggcgagatgg taattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac
     3361 ctggacgccg gacgatctcg cgccaccaca ggcagagttt gtgcccatgg tcgagccgga
     3421 agaaaccatc attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat
     3481 gcaggcccat gagcaaggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcatga
     3541 gcagggctat caggaaggac tggcccaggg gctggagcaa ggtctggcaa aggcgaagtc
     3601 tcaacaagcg ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct
     3661 tgatgcgctt gatagt
//
LOCUS       DADLTD010000283         3650 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLTD010000283 DADLTD010000000
VERSION     DADLTD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.287"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(245..451)
                     /locus_tag="JN701_003926"
     CDS             complement(245..451)
                     /locus_tag="JN701_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7366197.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQVGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(448..909)
                     /locus_tag="JN701_003927"
     CDS             complement(448..909)
                     /locus_tag="JN701_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366198.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(1280..2884)
                     /locus_tag="JN701_003928"
     CDS             complement(1280..2884)
                     /locus_tag="JN701_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366199.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNPISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHNFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(2897..3262)
                     /locus_tag="JN701_003929"
     CDS             complement(2897..3262)
                     /locus_tag="JN701_003929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366200.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(3290..3499)
                     /locus_tag="JN701_003930"
     CDS             complement(3290..3499)
                     /locus_tag="JN701_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366201.1"
                     /translation="MNKTPANIMDLYSLLLDHIRDISNRIANENTDIYKQFWNEDSYG
                     RTLTPKPEESCRNIFLDLLRVRLNP"
BASE COUNT         1027 a          799 c          786 g         1038 t
ORIGIN      
        1 aagccttaat ccatcggaag acagatccgg cagttttcaa aggcgacatt gccggtaaaa
       61 gggcaaccgt cctgatacat caggcgcttc tttcaggtca tgaagcattt cgtacaggcg
      121 ggagcttagt gcgtaacaaa gaaggccgat aataggaaac tgcttttgca gttcaaagga
      181 ttatgacggt tcggataaac aaaatgcggc gcagcttccg tatccgggct gcgccgcagg
      241 aacatcagcg gtagcgcgaa ggccagattt gctcaggctt gatgcctaac gcttcggcaa
      301 ttaacctttc gcctttgggc cagcggcggg taagcgcatt agccagcgtg gaggacgcca
      361 gacctacctg acgcgaaagc gctgacagcg aagtgcctcg ttttttcagc ccggcgataa
      421 tgtcggcagg atgccagtcc tgcagcatca tgcagccacc tcgtcgataa acttcacgcc
      481 atcggcggtg cggagccagc gcggggcttt catttcggcg gcaaaatacc agaccagttc
      541 acataataag cctgttatcg tctgctccac ttttggctgc atcccctgcc cgataagaag
      601 ttgcgccagc gtcagtgagt aatcacaaag taattctgcg tcggggacaa atcggggcat
      661 atcggcaggt agagcatcaa cggtcaggct gtcagtcaga tgagacggga tggggtcgtt
      721 cagcgtgggt tgcagcaaag tgagacaggc ggcaagtcgc ccgcaaagcg ccagccgcag
      781 caccggatct tcgctttcga tcagggtttc ggcgacgttt tcgcagtggt ctgccagtac
      841 ggtaaaatct gtggcagcac tgaagggaac agcaagtaac gggtgtgtct gagtggggat
      901 cgtagccata aaggcagcct ccagtatgtg gtttgaaaac caccaccaga ggttccaatc
      961 tcatgggtgg tggactgaac gaggttggaa ctaccggcca tactggaacc ggcgcacctt
     1021 acggtgcccc cgcccagccc accataattt tggtgtagct aagcgcgcga cataaaaaaa
     1081 gacgcagacg cgtcacgtgt cgccagtatg gttatatttc ggggttccaa tcccggcacc
     1141 tgattttgca ggtgcgggta aaagataccg ctttcggttg aaaacggcaa ggggaagtcg
     1201 tggaatagag atcgcagatt gttataaata tacacaatag ttaatgttaa caaagacggc
     1261 acatatatta actttactct caatctgctg aatagtttat cgcatgtaat tctcgggtca
     1321 tatagtggat accctccttc ttttctttca tcccctcttg cctcaccata aaagcaaacg
     1381 aaaaaattgc tgaacgagtt atctgaagta aatgctcaaa attgtgaata aaatcacttt
     1441 ctttgataaa aagaatatct tctattagtt gataagattc gtaaatgtcc tcaggtttat
     1501 cacttttatg tacaactaaa aacttatgct ctaaaccatt gcgccattct ttatagaaag
     1561 ccaactcccc acccttatct ttattcaggt cggtagcaat actgtacagc gccagaagcc
     1621 ctggtgattt taccttttca aataattgtc ttctgttatc aacatctaac tgccagaaat
     1681 tctgaaaata gatattcttc tgaggtgtac ctttttttgt tgttgggtaa agattataaa
     1741 gttcacatat ggctaccgca attttatcaa gaataccaaa gcaaagacga aatgccgtcc
     1801 tgattttttc aatttctatt cctaaaactt cgtcattata caattccaga aaatggcttt
     1861 cgtacttaat gtaatccgta ttttgtggat gaagataatc aaaataaaga tggcgtgcga
     1921 gtgaaaactc cgatttaagg cgatttaata ccatctccat gggaatgata aaatcacctg
     1981 ttacaccgcc tcgcgaagca atcacaagat catctgtagc actccctaca catgggcaat
     2041 acaggccatg ttctgataaa gtcagatgat ttctaagaca aaactgtcga tatgggctta
     2101 acacttcgaa ctcttgttgt gtaagtgctt catcgtgttc gtcagtttca tcatcaacag
     2161 catattctgc gattttctcg ttagtctgcg ctatgcgttc taaatagaac gattcccatt
     2221 gtggtgggat attttttgat acagacgctt taatatatcc tgctctcact tcttttagta
     2281 acttgattga aaatgaagat gaaataggat tgaggaccat aagtgacatc gaacggttga
     2341 cccaagcctg tggtatatct aatcccttaa gattggtaag atcataatat ctcaatgact
     2401 cactgagccg aaattgactt cttaatgaat tagccaaatt aacagatagt tcagcctgaa
     2461 attcctcctg ctcctcagca gatagcttaa aagaacgcca gtagatattt tttaaggaaa
     2521 ccaattcttc tatcgatttg aatgtatggt cattggggtt agtaatgctc accagattag
     2581 atagcgcatt accatagtta taatagtaaa ggcactctcc taacactgat ataaatgctt
     2641 ctttgttatc ttccatcagg ttgcaaccaa gctctgacgc atttttatta ttttgcatct
     2701 gcccgatgtc aacaagagca cccgcaatgt tcgccaatac tataaagtag ttatcgttcc
     2761 tgattgttat tctttccagt ttgttcagga acgcaactga ctgttgataa gattcgtcaa
     2821 atcttccaat atcaaccagt tggctaatga aattactttc atcgacaagt tcttttgtta
     2881 tcaaaataaa ttttccttat acagaggatt tcggtgcttc aacaggaggt atgccctcac
     2941 aggagacatc tataactatc gctgataagc ggtctctttt atcaaccggc acagtttcat
     3001 taagcatatt ttccattgca ctggcgtttt caggttgaga tgtatttttc gccaaatgag
     3061 gcagggaatt cggtcgagca gaaccaaacc aaaaaaccag atagataccg tgaccagcag
     3121 catcggggtt tttagtataa agcttgtcca attgcccatt aagtgcagtc cagacatcac
     3181 gatgataatc acgctttatc tcgataggta tttttatctg cggtagtgaa acaatgatat
     3241 ctgccctttt atcagaaacc atgtgtcctt ctggttcaca gcttattttt tatggattaa
     3301 gccggacacg gagtaaatcc agaaaaatat tacggcaact ctcttcaggt ttaggcgtta
     3361 gggtacggcc atagctgtct tcattccaga attgcttata aatatcggta ttttcattgg
     3421 caattcggtt agaaatatcc cgtatatgat caagcaataa tgagtaaaga tccattatgt
     3481 tggcaggcgt tttattcatc aatgtactta cagtctgctt ccagtcggca tgatggaact
     3541 gtgattctct ataacgaatt ttctgattgc tttgggcatg tgagagatga tcccgataac
     3601 tttcacattc aggcagcagt aataagcgct caagtacttc actagcatcg
//
LOCUS       DADLTD010000284         3587 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.288, whole genome shotgun sequence.
ACCESSION   DADLTD010000284 DADLTD010000000
VERSION     DADLTD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.288"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            344..541
                     /locus_tag="JN701_003931"
     CDS             344..541
                     /locus_tag="JN701_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7366202.1"
                     /translation="MNAHDIEPTILRATGNTHPLDVTFIHEEDLAIPWKPEQSSVYAH
                     VNPYGIFELDMETRLPIEVVA"
     gene            complement(623..2332)
                     /locus_tag="JN701_003932"
     CDS             complement(623..2332)
                     /locus_tag="JN701_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7366203.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="JN701_003933"
     CDS             complement(2764..3483)
                     /locus_tag="JN701_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY7366204.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1066 a          720 c          639 g         1162 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcaccct ctggatgtga cctttatcca
      421 tgaagaagat ttggccatac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtag
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaacatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgaga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac ggtcaggaat caggctgcat acgctgtgcc
     2701 aaatctaatc gtagttattc taaccagttt tccagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttc
//
LOCUS       DADLTD010000285         3540 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLTD010000285 DADLTD010000000
VERSION     DADLTD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.289"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            232..543
                     /locus_tag="JN701_003934"
     CDS             232..543
                     /locus_tag="JN701_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7366205.1"
                     /translation="MFNVITHPAALDEFLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="JN701_003935"
     CDS             543..830
                     /locus_tag="JN701_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7366206.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1367..2554)
                     /locus_tag="JN701_003936"
     CDS             complement(1367..2554)
                     /locus_tag="JN701_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7366207.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2554..2919)
                     /locus_tag="JN701_003937"
     CDS             complement(2554..2919)
                     /locus_tag="JN701_003937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366208.1"
                     /translation="MARSAKPRKRKSASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            2938..3333
                     /locus_tag="JN701_003938"
     CDS             2938..3333
                     /locus_tag="JN701_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7366209.1"
                     /translation="MTLPVFITVIADHDKPQPSGCLLELQLNKGEYRHKLPRWIFFAN
                     QGEFTTGDYEEIMNKASALSLVSNAILYWNTSRISNIVEGLRENGEVIDDDVLAHISL
                     LPYKHVLPNGTYFVDGEPYGTEPTERAKP"
     gene            3363..3446
                     /locus_tag="JN701_003939"
                     /pseudo
     CDS             3363..3446
                     /locus_tag="JN701_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            3446..>3540
                     /locus_tag="JN701_003940"
     CDS             3446..>3540
                     /locus_tag="JN701_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7366210.1"
                     /translation="MEQKWTSKALSDLARLYDFLVLASKPAAARMV"
BASE COUNT          927 a          918 c          835 g          860 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa tttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga gggggccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
     2641 ggtcgcaaat tcccgctccg tgcagcgggc aatttccgga tacccttcgt catcaacacg
     2701 tacaaaccag aagaccagtt ttttgtttcc ggcatccaca aagaacggaa tattcaggtc
     2761 tgcgcaacac tcaacggcat catcaaaact atcaaagcgc agaacttctg cgtcttcttc
     2821 gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgaga
     2881 ggcggattta cgtttacggg gtttagctga acgggccata taaccacctg aaagacaatg
     2941 acattgcctg tttttataac ggtaattgca gaccatgaca agccgcagcc gtcaggctgc
     3001 ctactcgagc ttcagctcaa taaaggcgaa tatcgacata aattaccccg atggatcttt
     3061 tttgcaaatc agggtgaatt cacgaccggt gattacgaag aaatcatgaa taaggccagc
     3121 gccctgagtc tggtatcgaa tgcaatcctg tactggaata catcaagaat aagcaatatt
     3181 gttgaagggt tacgtgaaaa tggtgaggtt attgatgatg acgtattagc ccatatatca
     3241 ttactgccct ataaacacgt attacctaat ggtacttatt ttgttgatgg agaaccttac
     3301 ggtactgagc ctaccgaaag agcaaaaccg taagtttctg ttcaaatgct cccgctggtc
     3361 catccggaca gattatcgac catcaggctg tacaggcctg ggcggacagc ctcagtactg
     3421 acaatccgtt accggtgcca cgctgatgga acagaagtgg accagtaagg cgctttctga
     3481 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaatggt
//
LOCUS       DADLTD010000286         3529 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLTD010000286 DADLTD010000000
VERSION     DADLTD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.290"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(293..988)
                     /locus_tag="JN701_003941"
     CDS             complement(293..988)
                     /locus_tag="JN701_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY7366211.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(981..2408)
                     /locus_tag="JN701_003942"
     CDS             complement(981..2408)
                     /locus_tag="JN701_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY7366212.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2419..3138)
                     /locus_tag="JN701_003943"
     CDS             complement(2419..3138)
                     /locus_tag="JN701_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY7366213.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          896 a          968 c          740 g          925 t
ORIGIN      
        1 gccttgtcaa aaaaatggta gatgaatacc aacaaaactc tgggcagtaa taaacaacat
       61 tctgatagag agtgcctgta taaagctgaa gtgacaatga ccgggaaggg aacccgtttt
      121 caggaatgga tgcctgtctt tttcactgta aatatcctgg cattactaat gccaggatat
      181 ttacagaaga gcagcataac catgacggaa cggggttaag tcaggttgtc cttaacgcta
      241 ttttcaacct cgtatgcctt cttcaggttt atgttcagat ctcatatctc tctcagcaat
      301 cctcaataat cagatacacc gctttcaccg ggccatgaac cccgacgact ttgataagct
      361 caatatccgc cgttgaacta gggccgctaa tgatgttaat gcaggacggc attcgttcac
      421 cggcctgcgc tttctgatgc aatttttctg cgagttgcgc tacacgcggc aggattgtgc
      481 ttttacgcag gataaaaaga gaagattccg ggagcaggct caatgaacgc ccgcgctcgg
      541 cggcggaaaa aagaaccacg cctcccgatt cggttaaacc atattcagca tacacaacac
      601 ccactttagc ctgctctgcc tgcgagatat tctcggcacc tttcgccgga tcccaaacaa
      661 cggcattgca ttcctgctgc aaacgttcac taatccccaa ttcctccagc ctcgtgtcac
      721 cgctaatcac gaccgactga tctcccagct ctttacacag acgtattgca gcttctgccg
      781 ccttcgcctc gctggtcagc tcacagcgcg tcaacataac atcgctggca aactgaataa
      841 acgcgtcaca gcgctgctgt tggttaagtt gggtaagccg ctcgttagca tagttgttaa
      901 gcggcgcatc ttctgcttgc ggttcaagtc gcagcgggcg acccagtgcc tgagcaacgt
      961 tattcaaaaa ttcgcctcga ttatccattc tttttctcct gcgcctgatg tttcttaaac
     1021 caactacgga aactctctcc gtcagcttca ggaagatcgc gtgcttccat ccagtcgcta
     1081 atcgcgccaa atttgagtgg tgttttgccg ccattgataa accagcttgc cgcatgagcg
     1141 ccggccatca tcccgacttt ccacaatcct ggatgactat tggcataagc gaacattttt
     1201 atcgcccgtt gctctgcttt tgcggtgatc cctttttcag ccatcacccg acgatgacgc
     1261 aaaatcagtt ttgacagcgg aatacgcacc ggacacacgc tgtcacaagc agtgcataaa
     1321 gagcaggcgt agggtaaatc tttaaaatct ttatagccgc caagtagcgg agaaatcacc
     1381 gcaccaattg gccctggata aatagagcca tatccatgac cgccaatatg gcgatatgcc
     1441 ggacaagtat tcatacaagc cccgcagcga atacagcgca gcacatcccg aaattcagag
     1501 gccagcacct cagaacgccc gttatcgaca ataaccagat gaaactcttc aggaccatca
     1561 acgtgccccg cttcgcgcgg tcctgtcagc caggtgttgt atcccgtcaa acgtgcacca
     1621 acggcactgc gcgccagcat ggtgatcaat acatctacct cggcaaacgt gggggcaata
     1681 cgctccattc ccatcactgc aatatgcgtt ttaggcagcg tggtacacat tcgcgcatta
     1741 ccttcattgg tcaccaggca taccgaacct gtctctgcca ccgcgaaatt acagccggta
     1801 atacctattt cagcactgag gaaatcttcg cggatttttt gccggatgaa taaggtcatc
     1861 gcttcaggcg tttccggccc ctcatagccc agacgttcgt gtagcactcg acggatctga
     1921 tggcgatctt tatgaattgc cgggaccaca acatgagatg gcggatcttg atccagctgg
     1981 agaatatatt cacccagatc ggtttcaatc acctgaatgc cagcatcctg caacacatga
     2041 ttgacaccaa tctcttcggt caccatcgat ttagatttca ccaccttccg ggcatttttg
     2101 cgttgggcaa cctgtaaaat gtagcgggta gcgtcttctt tgttttttgc aaaccagacg
     2161 tgaccgccgt tttgcgtcac tttttctgag agctggtaca gataagcgtc gagattactc
     2221 agaacatgat cacgtatctg ggcggcccga tcgcgccact cctcccagtg ccccaattca
     2281 tcgaccattt tttgccgatt tgccccaata cgctgctgcg cgtttgccac cgctttgcgc
     2341 atgatcggat cttcaatttg ctgacggatg cgagtcttaa aatctgtatt actggttttg
     2401 atcgacatct ttatatcctc agcggctcat caacacttca gcaatatgca tcactttgac
     2461 tttctgccct tcccgttgta atcgcccact gatgttcagc aggcaactca cgtcagcacc
     2521 aattaaatac tcagggcgga cttccatcag gtgcccaacc ttttctttca ccatctcgcc
     2581 ggatatttcg gccattttga ccgagaacgt gccgccaaat ccgcagcagg tatcctgttc
     2641 agcaaaggtc aacagctcca gtccacgcac atttttcagc agcgtaagtg gctcgtcctt
     2701 cactcccagc ttacgggcca ggctacaaga tgggtgatac acagctctcc cttgcaaact
     2761 ggcacctaca tcgactaccc ctaatttatt aacaataaaa gaggtgagat cctgcatacg
     2821 cgcggcaacc ttttcggcac gtgatgccca ttcaggttca tccgccagat acgtcgggta
     2881 actttttacg gcataggtgc aagagccagc cggtgaaata atgggatcgt cgttatcctc
     2941 cagtgcggcg atcagatttt tcatccctgg aatcgcttct ttgatataac cgctattgat
     3001 cgcaggctga ccgcagcatc cctgtttctc cgggaaattt acgcgacagc cgagtttttc
     3061 cagtagcagc acggagtctc gtgccattct tgatttcagg gcgtcaccaa tacaggtgac
     3121 aaagaaattg acattcacaa ctaatactcc attacttcat gcccatttat gcccgatgtt
     3181 tcccccccac cgggggatca atcatcaata aatatggcgt tcttttttaa ttggctagca
     3241 acatactacc cctcatttgt atgacaaatg ataatctttc acccaaacgt caaatgcaat
     3301 aactaatttt atttctttcg gcatcgctca cattttatgg agaaatcgac gatcattttt
     3361 ggcataagaa atccatttaa aaatggccac atcccttata cctattcaga gctacttatt
     3421 caggggttat tgagatatac actgatggtg tacaaacaaa atcaccgaca ataatcacgt
     3481 atcaagtaat agtcgggatt ttatttaatt gaagcaattt agtttgtat
//
LOCUS       DADLTD010000287         3484 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.291, whole genome shotgun sequence.
ACCESSION   DADLTD010000287 DADLTD010000000
VERSION     DADLTD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.291"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(121..1575)
                     /gene="ospC2"
                     /locus_tag="JN701_003944"
     CDS             complement(121..1575)
                     /gene="ospC2"
                     /locus_tag="JN701_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY7366214.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
     gene            2000..2844
                     /locus_tag="JN701_003945"
     CDS             join(2000..2342,2342..2844)
                     /locus_tag="JN701_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAY7366215.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALD
                     GSNIRALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAH
                     ESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNE
                     KMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFY
                     QRLRN"
     gene            2959..>3484
                     /locus_tag="JN701_003946"
     CDS             2959..>3484
                     /locus_tag="JN701_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY7366216.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEH"
BASE COUNT         1016 a          729 c          715 g         1024 t
ORIGIN      
        1 ggggcgttca ccacatgcaa tgtgttgttg caggggaacg gtctgcacca tcccctatat
       61 cgatcaatat aacaaaaatg acaacgtcag attcagggca caacctaaat atattttagg
      121 tcacctccca aatcgtttgc ctgataccgc tccatttctt aataaaaaat caattgtttt
      181 tgaatctttg cttttcattg cattgtcaag catagtgtcc ccactatttg ctttttgaaa
      241 tcttttgttt acatcaacca atccattatt aataaaatat tctagcactt tatagttggc
      301 actatcttca ctaagtaaat actcaacatc atacagctca caatacatat tgttgttcat
      361 tttctccatc tctgcgatat cttgttttgt aaaattaaat ttagatagta aataaagtgc
      421 catatcagat tttgcattgc ctagagaata agcaagagct tgatgagcca tttttttatc
      481 agtcacctta ttgttaattt cttcataatt ggatgcttta atggcatcct ccaaagatat
      541 atctctgagt cttatctttt tgaagttatc tgtgcttagc attcttggta tatgatactc
      601 aagctgaaac acaaagttta taattctatc cagactaaca ggatcaatgt ttttattata
      661 acaaaactct cggaatcttt gatctttact ctttcttata aaatcaataa gatgcaaccc
      721 aattccaagc ctcatatctt tagtattaaa tattggcaca tcagtttttc cctgattacc
      781 atgcacatac ctgctaactt catccacaac ctctttaaag ttatctaaga aaaatgattg
      841 gtgctcatgg taaaaaacag gtggaatagc attatcaaag tggtcggtta atgtcatata
      901 tccatatgga gagtcatgat caatgatata cgcattagca ccaaaatcca cagtatgatg
      961 tgttttattt agtgggagtg tttcttgatg attggaaatt tcaaccccaa aaaaaacaaa
     1021 atcgtggtta gctatttctt caatatcctc agagaatgtg tttttatatg tttgtatatt
     1081 ctcagactta attttatcaa aggacttaat attaagagta ttatttgaca ggttagcgtt
     1141 actttgatgt ctgaagttaa aatcagaatt aataataata gagagcaagt gacgttcatc
     1201 cgaagaaaga ggatgagttt tttgtattat ttcagaaaga tagtgtctgg acaccttatc
     1261 gaggtgctca agtgaccgaa atgatgaccg tgctccagat ggcgcttcaa tagaaagatt
     1321 taatgatttg aaactggtcc cttgagagaa cattttacta taactttgcg ctgctataga
     1381 cttccttaaa aagcttttca cagtatcagc acagtgatct aaatgttttt cacttatgcc
     1441 agaagaggat gagtttgtta cgcatgatat gtttttttgt aatgcttttg tatctttgtt
     1501 cggatttatt tttccatcaa caagatcaat attgttctga acattaatat gatttactgc
     1561 ttcaggtatt ttcataaaac atcccccatt atgtgcgttt ttatctgcag ttatttagaa
     1621 taatattatc taacttaata ttttcttgta tgtattataa cgaacatatt ttaatatgtt
     1681 gtacgatttt cactctgaaa tcccctgagt ttaatcacac ttcctttact gaaaggaatt
     1741 attgcaacag tattatttgt ttttggatat atatcttgcg ccttgttatg tagttaatgc
     1801 tcagaatatg atagataaaa tagagacgca gaacagggca tgctcataac atgatgataa
     1861 cataaaaaat tagtaacaat aaatgaaaga atcatgggcg ctcaacaaaa agagagctaa
     1921 atagctgcgc ggaatagtag gctgtgtccc ttaatcatct gagttatagt aactgtctgt
     1981 ttacacaaca tattgaacta tggctcgcta cgaccttccc gatgaggcat ggaccatcat
     2041 caagcccctg ttacctcctg aacccgccac accacgggcc ggacgcccat gggctgagca
     2101 ccgtaaaatc atcaatggca tgttctgggt gttatgttcc ggtgcaccat ggcgcgattt
     2161 acccgaacga tatggggcat ggaaaactgt ttataaccgc tttaaccgat ggtcaaagtc
     2221 aggcgtgatt aatattattt tcaacaggtt gctttcgcta cttgatgcaa acggctttat
     2281 tgactggtct gccaccgcgc tggatggcag taatatccgg gcgctgaaat gtgccgccgg
     2341 tgctcaaaaa aacatcccga tatcgacgga gataatggcc cgggtcgctc tcgcggcggt
     2401 tttggcacca aaatccatct ggcaacagac ggaagtggcc tcccgttaaa tatcgtgcta
     2461 agccccggac aagctcatga aagccagttc gcgcaacgac ttctggacgg gattggcgtt
     2521 cagcgtcaga acggcagcat gaaacgccgt ggacatgcgg tactggctga taaagcgtat
     2581 tcagggcgcg cattgcgcaa cgagctgaaa aataacggta taaaggcagt aatcccccga
     2641 aagtcaaatg agaaaatggc atcggatgga cgtgcacagc ttgatcgtga tgcgtactgc
     2701 aatcgtaatg tcgttgaacg gtgctttggg cggctgaaag aatatcgccg catcgccacg
     2761 cgttacgaca aaacggcgag aaattacctg gcgatggtga aactgggctg catccgactc
     2821 ttttatcaac gcttacgtaa ttaagggaca cagcctaaat agctgcgcgg aatagtagat
     2881 ccctttgagg gaacttagcc cggattgtgc gatctgatca atcgccaaat caaaacaaat
     2941 caccaaccgg actgagcaat gccgatcata gcaccaattt cccgtgacga acgacgcctg
     3001 atgcagaaag ccatccataa aacacacgat aaaaattatg cccgcagact gactgccatg
     3061 ctgatgctgc accggggcga ccgtgtcagc gacgttgcca gaacgctctg ctgcgcccgt
     3121 tcctctgttg gacgctggat taactggttc acgcagtcgg gtgttgaggg actgaaatca
     3181 ttacctgccg ggcgtgcccg tcgctggccg tttgagcata tctgcacact gttacgtgag
     3241 ctggtaaaac attctcccgg cgactttggc taccagcgtt cacgctggag tacagaactg
     3301 ctggcaataa aaatcaatga gataaccggt tgccagttaa atgccggaac cgttcgccgc
     3361 tggttgccgt ctgcggggat tgtgtggcga agggctgcgc caactctgcg tatccgtgac
     3421 ccgcataaag atgaaaagat ggcagcaatc cataaagcac tggacgaatg cagcgcagag
     3481 catc
//
LOCUS       DADLTD010000288         3481 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLTD010000288 DADLTD010000000
VERSION     DADLTD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.292"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..1944
                     /locus_tag="JN701_003947"
                     /pseudo
     CDS             1..1944
                     /locus_tag="JN701_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(1987..2460)
                     /gene="ivy"
                     /locus_tag="JN701_003948"
     CDS             complement(1987..2460)
                     /gene="ivy"
                     /locus_tag="JN701_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY7366217.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2614..3384
                     /gene="yafV"
                     /locus_tag="JN701_003949"
     CDS             2614..3384
                     /gene="yafV"
                     /locus_tag="JN701_003949"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY7366218.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          777 a          890 c         1000 g          814 t
ORIGIN      
        1 attatcaaaa aagagtacgg ctgactggag ttctcagctt atgcccagtc tcgcgtgctg
       61 caaaaactct ccggcgtgag cgggatcctg gcgattaccg tcggcgtgcc gaactcatta
      121 ggcccgggcg agctgctgca acattacggc actgacgagc agaaagatca ctatctgccg
      181 cgtctggcgc gtggtcagga gatcccttgc tttgcactga caagcccgga agcgggttcc
      241 gatgcgggcg cgattccgga caccgggatt gtctgcatgg gcgaatggca gggccagcag
      301 gtgctgggga tgcgtctgac ttggaacaaa cgctatatta cgctggcacc gattgcgact
      361 gtgcttgggc tggcgtttaa actctccgac ccggaaaagc tgctgggtgg cgcagaagat
      421 ttaggtatta cctgtgcgct gatcccgacc accacgccgg gcgtggaaat tggtcgtcgc
      481 cacttcccgc tgaacgtacc gttccagaac ggaccgacgc gcggtaaaga tgtcttcgta
      541 ccgatcgatt acatcatcgg tgggccgaaa atggccgggc aaggctggcg gatgctggtg
      601 gagtgcctct cggtaggccg cggcatcacc ctgccttcca actcaaccgg cggcgtgaaa
      661 tcggtagcgc tggcaacggg cgcgtatgct cacattcgcc gtcagttcaa aatctctatc
      721 ggaaagatgg aagggattga agagccgctg gcgcgtattg ccggtaatgc ctacgtgatg
      781 gatgctgcgg catcgctgat tacctacggc attatgctcg gcgaaaaacc tgccgtgctg
      841 ttggctatcg ttaagtatca ctgtacccac cgcgggcagc agtcgattat tgatgcgatg
      901 gatatcactg gtggcaaagg cattatgctc gggcaaagca acttcctggc gcgtgcttac
      961 cagggcgcac cgattgccat caccgttgaa ggggctaaca ttctgacccg cagcatgatg
     1021 atcttcggac aaggagcgat tcgttgccat ccgtacgtgc tggaagagat ggaagcggcg
     1081 aagaacaatg acgtcaacgc gttcgataaa ctgttgttca aacatatcgg tcacgtcggt
     1141 agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg gtttaaccag cagcacgcca
     1201 accggcgatg ccactaaacg ctactatcag cacctgaacc gcctgagcgc caacctcgcc
     1261 ctgctttctg atgtctcgat ggcagtgctg ggcggcagcc tgaaacgtcg cgagcgcatc
     1321 tcggcccgtc tgggggatat tttaagccag ctctacctcg cctctgccgt gctgaagcgt
     1381 tatgacgacg aaggccgtaa tgaagccgac ctgccgctgg tgcactgggg cgtacaagat
     1441 gcgctgtatc aggctgaaca ggcgatggat gatttactgc aaaacttccc gaaccgcgtg
     1501 gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac gtcattatct ggcaccttct
     1561 gacaagctgg atcataaagt ggcgaagatt ttacaagtgc cgaacgccac ccgttcccgc
     1621 attggtcgcg gtcagtacct gacgccgagc gagcataatc cggttggctt gctggaagag
     1681 gcgctggtgg atgtgattgc cgccgaccca attcatcagc ggatctgtaa agagctgggt
     1741 aaaaacctgc cgtttacccg tctggatgaa ctggcgcaca acgcgctggc gaaggggctg
     1801 attgataaag atgaagccgc tattctggtg aaagctgaag aaagccgtct gcgcagtatt
     1861 aacgttgatg actttgatcc ggaagagctg gcgacgaagc cggtaaagtt gccggagaaa
     1921 gtgcggaaag ttgaagccgc gtaattgaat aacggagccg aaaggctccg tttctttatc
     1981 cgctaattat ttaaaattaa agccatccgg atggttttcc aggctgccgg tcaacgccgc
     2041 gaacagcacc gttttaccat caatcgaaag cgcatcgttc acattcagcc aggtgagttt
     2101 ctcttgcgac gttttctcat caatagtcga gaacagcccc gtcatctgat tagatttctc
     2161 ggaccacatc acagcgatac gttgcgagcc acagtcatgc ggtttgcacg cgctcatcac
     2221 ctgatacgtc tcatctccca acgttacggt ttgtgcggga gtataagtac cgcctttcat
     2281 cacccaggca ggcagcttat gcccttgtac catctgatta aatgcagctt tggtggtttc
     2341 gccctttgca aggctgctaa tggttaaatc atcctgcgcc attgcactgg tggcgatgac
     2401 cagagcggcg actgtcgtta ttgccttaaa catcattcct cccgagctta tcctgcccat
     2461 gttattaacc ttctaaagcc tggcgataac acgataattt gcaaatcaga tatttccaat
     2521 atcagcattt gttatcaatt tattctgcgc taaggcaact gcctgataag gtgttaaagt
     2581 tataaaaccc ttcgcataaa ggagttgtac accgtgcctg gtttgaagat tacgcttttg
     2641 cagcaaccac tggtgtggat ggatggccct gccaacctgc gtcattttga tcgtcaactg
     2701 gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc
     2761 atggaagcgg cagcttcgtc gctagcacaa gatgacgtag tgaactggat gacagctaag
     2821 gcgcagcagt gcaatgcgct gatagcaggc agtgttgcat tacaaacgga gtctggttcg
     2881 gttaaccgct ttttgctggt tgagccgggc ggcacggtac atttttatga taagcgtcat
     2941 ctgttccgca tggcagatga gcatctacat tataaagcgg gcaatgcgcg agtgattgtg
     3001 gaatggcgcg gctggcgtat tttgccgttg gtgtgctacg acttacgttt tccggtgtgg
     3061 tcgcgcaatc tcaacgatta tgacctcgcc atctacgttg ccaactggcc tgctccgcgc
     3121 tctctgcact ggcaggcatt gctgacggcc cgcgcgattg agaatcaggc gtatgtggcg
     3181 ggatgcaatc gcgtcggcag cgatggcaac ggctgccatt atcgcggtga cagccgggtg
     3241 attaatccac aaggcgagat tatcgctact gccgacgcgc atcaggcaac gcgcattgat
     3301 gcggagctgt cgatgatggc attgcgggaa tatagagaaa agtttccggc atggcaggat
     3361 gcggatgagt ttaggttgcg gtgagtgggg gttgtgttta aggctggggg agagtcgggg
     3421 taagactacg aaagcccgct ctccgcaagg actgacgcca gatagtttct gtccatagct
     3481 g
//
LOCUS       DADLTD010000289         3418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLTD010000289 DADLTD010000000
VERSION     DADLTD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.293"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            57..272
                     /locus_tag="JN701_003950"
     CDS             57..272
                     /locus_tag="JN701_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7366219.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            277..621
                     /locus_tag="JN701_003951"
     CDS             277..621
                     /locus_tag="JN701_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAY7366220.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            672..1205
                     /locus_tag="JN701_003952"
     CDS             672..1205
                     /locus_tag="JN701_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY7366221.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1361..1543
                     /locus_tag="JN701_003953"
     CDS             1361..1543
                     /locus_tag="JN701_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366222.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1701..1875
                     /locus_tag="JN701_003954"
                     /pseudo
     CDS             1701..1875
                     /locus_tag="JN701_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371701.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
     gene            2108..2332
                     /locus_tag="JN701_003955"
     CDS             2108..2332
                     /locus_tag="JN701_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366223.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            2677..3003
                     /locus_tag="JN701_003956"
     CDS             2677..3003
                     /locus_tag="JN701_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HAY7366224.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(3135..3335)
                     /locus_tag="JN701_003957"
     CDS             complement(3135..3335)
                     /locus_tag="JN701_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAY7366225.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT          849 a          733 c          945 g          891 t
ORIGIN      
        1 gtgtgtgccg gtggtcttta tattgttgtg agcttccgga ttgcgggaga cggggtatgt
       61 accagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggtg ggaacgggct
      121 actggttcct gcagttgctg gacaggtttt ccccgtctca gtgggcggca ataggcgtgc
      181 tggggagtct gttgtttggg ctgctgacat atctgactaa cctgtatttc aaaatcagag
      241 aggaccgtcg taaggcggcg cggggagagt aaagcgatga agaaaaaata cgaactggtt
      301 gttaaaggga taaataatta cccgaataag attactgtta ctgtggcact ggaaattggt
      361 gggtatccgt cactgttgtt gccagatgtg gcgattagtc ttgaccgtac tgaagatgcc
      421 acgctggagt tttacgaagc tgaggcgaaa aagcaggcga agcagttttt catggatgtt
      481 gctgccgggt tatgtgaagg ggatggtccg ttgccggaaa agcgccccgt aattttagag
      541 gcgcaggatg tgttgataac ctacagagga aaactaccgg gaataattac tggttctctg
      601 aagactccac cgctggcctg aagacttaac atatccaggg atttgaaatc gataaaccct
      661 gataaatatc catgaacacc aaaatcaaat acggcctgtc ggctgccgtt ctggcgctga
      721 ttgccgcagg tgcgcctgcg cctgaaatcc tcgaccagtt tctggatgaa aaggaaggta
      781 accacaccac ggcataccgt gatggcgcgg gtatctggac catctgccgc ggtgccatcc
      841 tggtggatag taaacctgtc gtcccgggca tgaagttgtc gaaggaaaaa tgcgaccagg
      901 tcaacgccat tgagcgtgat aaggcgctgg catgggtgga gcgcaatatt aaagtgccgc
      961 tgaccgaacc ccagaaagcg ggtatcgcgt cattttgtcc ctataacatt ggccccggta
     1021 agtgtttccc gtcgacgttt tacagacgga ttaatgctgg tgaccgcagg ggagcatgcg
     1081 aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgc tcaaataatt
     1141 gctacggtca ggtatcccgg cgagaccagg aaagtgcgct ggcgtgctgg gacatcgaca
     1201 gataagcaga atattttgct gaaaataagg catgagcacg cggacggata acacgaaatc
     1261 ctgcgaactg gcgaaacgta agtgaataaa agtaaaaacc ccgtttgttg gcaccaggcg
     1321 gggttttgtg tttctgacct tgagtaaggc aagggagaac atggcgaagt ataaacgaat
     1381 tctgttgagg ttgaccatga aaaacggcct tgaactgaaa gcgcctgtaa ctgatgacat
     1441 cagcagagca ctggcttttg ccattaagtg ggtggcggtc ggtgttgctg tgtccccgat
     1501 gctgtatggg ctggcaaaac tggtcattgc gttgaaatcg tgaagggagg attaagcatg
     1561 tcagacaaac tcataacgct ggcgaagatc ctctgtgtaa ttgtcggcat ttcattttca
     1621 ctaatgctgg ttgctctttt tctttccatg gcctggatga tgttgtcttc gtcggggctg
     1681 ctggggtgaa cataaaccga atgctttccg cgtttaccgt tattctgctg gtggtctgtg
     1741 gtgcgctgtg gctggcaaca gaccattacc gtgataacgc catcacctac aaagcgcagc
     1801 gcgataaaaa agccagagag ctggagctgg caaacgcaac cattactgac atgcagcagc
     1861 gccagcgtga tgttgccgct ggtcgtaagc gcctgcggat caacgccacc tgctccggta
     1921 ccgtgcgtga agccaccggc acctccggaa tggataatgc aaccggcccc cgactggcag
     1981 acaccgctga acgggattat ttcaccctca gagagcggtt gatgacaatg cagaagtaac
     2041 tggaaggggc gcaggaatat atccgcactc agtgtattaa ctagtatttt tgttatccgg
     2101 agaatgcatg aagaaattac tggtaaccgt aaagcctttt cagggaacaa ttccgttccg
     2161 tattttgcag cgtggtcgtg ttcttgttga aggttcgttc agtggtaaat gtacgcaatt
     2221 acactcccgg acctttcagg tgaatgccac gaatgaagag ctaaccgttg agtgtacgat
     2281 gaatgccgct aaatgccgca tggtatccgc tgcattacag ccagtgtgtt gagcgacctt
     2341 attaaccatg cgcggtattg tcgccgtatc cccgcattaa cagagaccgc agcccgaccg
     2401 ggagactcct ctgcgcgagt gtgcggggat aatcaaaaac gatacacacc ggggtttacc
     2461 gcgttaacgg agcgcggcgt tgtcccctca tggtcgctgg tccggtgcga tggtggaaga
     2521 aaccggacga tgtgttacct cgcaagctct gttatgtcat gtgtctgatt tgtgatttaa
     2581 gtcggataat tgtcgttgcc attaagcaga ggattgatga ccgacagggt ggcattgtta
     2641 gaataagact tattcttatc tgtgccggga atgaaaatga aaagaaacct tccgttaatt
     2701 attttgttgt cttctctggt tatgggctgt acgcaacata aaacagatat gccccgacag
     2761 ttggttaagg cattaccaca atatccggcc tatgcagcgg caaattatat aaagggacgg
     2821 gttgatgtga agtttgatat tggtgctgat ggtactgtca cccgaattga gtttatccgt
     2881 tcagagccgc accatttgtt tgatgagcag gttgtaaagg cgatggcaaa atggcgattt
     2941 gagaaggaca ggccgtgtaa aggcgtgaag aaaacgttta tctttagtcc ttctgcaccc
     3001 tgattatttc atcagaaatt aattatcact ctgttgttat tctgtacatc ccggcagggt
     3061 aagtcttgtt ccgtcggata tgaagatgaa atattgttgg aggacagtgg gtacctgctc
     3121 ctgtaaccga acgttcattt ctcgttattt ttcatgctgg ccgggcgcag atgcgttgca
     3181 tctgttgcca gccttctcct gcaggcttca ataacccacg ctgaaaagtt tcctgaacct
     3241 ttcagatcaa gagcgatgtt aatttgttca atcatctggt ttggaaatcg gatgttgcgg
     3301 gttgttgttc tgcgggttct gttctttgat gacataatgt ttccccatat tcagtgttgc
     3361 tgatttgtat tatctgaagt tgcttttacg ttaatttgat gcagatcaat caatacga
//
LOCUS       DADLTD010000290         3413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLTD010000290 DADLTD010000000
VERSION     DADLTD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.294"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(21..323)
                     /locus_tag="JN701_003958"
     CDS             complement(21..323)
                     /locus_tag="JN701_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366226.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(323..1033)
                     /locus_tag="JN701_003959"
     CDS             complement(323..1033)
                     /locus_tag="JN701_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366227.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGGTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1030..2028)
                     /locus_tag="JN701_003960"
     CDS             complement(1030..2028)
                     /locus_tag="JN701_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7366228.1"
                     /translation="MASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDW
                     LDSHGFFWASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQA
                     TGREAAFGESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPP
                     GLLRSVAITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKL
                     GGLIRFFHGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIR
                     SLHPAPVSQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(2555..3052)
                     /locus_tag="JN701_003961"
     CDS             complement(2555..3052)
                     /locus_tag="JN701_003961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366229.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRHCKCKSGLQ
                     SRKVL"
     gene            complement(3036..3218)
                     /locus_tag="JN701_003962"
     CDS             complement(3036..3218)
                     /locus_tag="JN701_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7366230.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          796 a          996 c          814 g          807 t
ORIGIN      
        1 tattttacgt tgtctgtagc tcatatcagt tcctcaggta acagccagta gagccggtac
       61 cgggatccga gcccccgcca gtcagggtca ctattcccct ccgtgtcggt aaaaaacagg
      121 tccccctgaa acggcaggta tccgtaccgg acaatccggt tattattcag acacagcacg
      181 ccatacaggc acggttcacc attaacaatg atatcgatat acatccccgt agtacgctga
      241 ttcaggcgaa tggtgcagac ctgagcgccc agtgtcaccg taaactgctg ggctttaacg
      301 ggagataaaa caatttccag catcaggtga tccccctgtt cgtggcactt cgtctgtcag
      361 cgtctgacgg ttgtgtcacc gacgccgtaa ctggctgcgt tttaaccgat gcggcccctt
      421 ttgctttatc gttgtccgtg ggagacgact tatccgtact tcccactgac acctctcctg
      481 tattcattac ggtctgaaat accgcagtaa ccgtcagtaa ggtcggacca ttattacttc
      541 gcattcggta gtcatatttc acaaggtcgc aggatgtcca tgtcttgtcc ggcgtctcaa
      601 tatcgtagag tcctgccgtg gtacgcatcc tttcaagcgt ttccagcaca ttcgtccgcg
      661 aggtggtgga aaaatttgtc aggttcggga cggatcccga aaaggccgtc cccccctcta
      721 caatgaaagt cacatgcagt tccggcgatc tctggatttt attaaaggtg gtataagccc
      781 cctgctccac ggggacggtg gaaacagaag cctccgcgcc cacctcaacg acaacaaaag
      841 aatccgggga gaaaggtttg ccacccttct gatgggcacc tgccggatca ttccgtgcgt
      901 aataaatacc gaatgacggt gccagtacac tattaatgag tcccaggaca ccgccgccac
      961 gaactgcact cagtacgtta ctttcactga gcgaaaagtt attcagggaa atattatcga
     1021 aagaaaaact catcctgtta ccccgctgga ataaaccgac acaagcgccg aattcgtgat
     1081 acgccgacgt gcgtcatcgg taataccctt cacattgtcc gacgttgtgg tgacattcag
     1141 tgtcccgata tgcgtggttt ccgttacggt ggactgcgat acaggcgcag gatggagcga
     1201 ccgtatggcc attgccgcac ccggataagg caggttcgcc agcacccgcg gaatatagtt
     1261 gcgggtctcc tccggagcag cagccagtcc cttacgctga acatttccct ctccccagtt
     1321 atatgccgcc agagccttag ccagatcgcc atgaaaaaaa cgtatcaggc caccaagttt
     1381 tctcgcggcg gcatcagcgg ctttttcggg atcaaaggca tcgttccccc tcagaccaaa
     1441 ttcactggcc gtcttcggca tgaactgaaa cagtcccatc gcaccagcag gtgagacggc
     1501 aaactgatta ccacgggatt cggtgatcgc aacgctgcgc agcagtccgg gcggcaggtt
     1561 atatttttcc tccagttgag acagtttcgg ttgcagccag cctaacaggg cttccccggt
     1621 cttcgtcggt cgctggcggt tttgcatggc attaccgagt ttttcctgcg tcgcacgcat
     1681 accctgtagc caggattcac cgaaggctgc ttccctcccg gttgcctgag aagcctgagt
     1741 atccagcatg ccctgctgcc atactgtggg tgattgtgcc cgggtgatgt tgccaggctt
     1801 ttctccggca tccagttttg tctgatacgt ctccatctct ttcttattga aaaagaaagt
     1861 cccgtctgaa gcccagaaaa aaccatgtga atccagccag tccttatttt ccctgccaac
     1921 aatggatgtc agtaatccgt caacaaccgg gtaaagcgcc gttatcgcaa aaagaaggcc
     1981 tccggggcca ctgagggctt ttgtcagccc ccgcacccat gacgccactt tcagcccgat
     2041 cagcgtaata atgacattct gccagccccc cagttctccg gcagccttat tcgccaggga
     2101 ggccactgac tcaacgtcat tcagaaatgt ggtgataaac ctgttcactt cctccggatg
     2161 ttgctgcatc cagtcaccga gtgtctccag ccagtggctg agcttcagcg cgtacggcat
     2221 caacgcggtg ctaatcgtca tcccgatgcc ggaccaggtc tgatcgagct caacaagctc
     2281 ttttcgtagc ttacgggcca gcgctgtacg ccccggcgta acctgcgaac gggaagtaaa
     2341 ttcgtccaca tctttcagcg catgaccgga gccaagaaac tgctgtccgg cataactgaa
     2401 gcccagcgcg ttgccgtagg cagtttgttc ggactttgtc agtcgcggaa aaataaccgc
     2461 cagcttgcgc atgatggctt cagtgctgtc agtatttaaa tcaacactaa tgcccgccct
     2521 ggctgcaacc tgaaacaaat cctgtaacac aggatcaaag gaccttccgg ctctgaaggc
     2581 cgcttttgca tttgcaatgc cgggaaaatg ccccagacat ttccccttca tcaacattat
     2641 ttgcccgcgc agcccgtaac catccgtcca gatttctggc cttcatgcca aaagcatcgg
     2701 attccacgga cagacggttc agatcacggg caaaacctgt taccaggttc ttcagccccc
     2761 ccagcgtcag actgacaccc gcaagagcca gaatctcgct acggatccct gaaaaaaaac
     2821 gggacgcccg tttcccggca tcttccattc gacgggccgt tttatcagca tcttttccgg
     2881 ttttttccag tgattcactg gttttcttct gtccctgact gaatgtgtca gcgacacctt
     2941 ccatcaccgt cgtgagccgg ttaagggcag catgtactgc ctgttccccg gcagtaaagt
     3001 ccctgtcatc aatcccgagg gacagaacaa gctcatcaag tacctgcgcc atttctgttc
     3061 tcctgtatca cctgttcgtt atgggcatcc acatgaatta tttccagcag atcccataag
     3121 tcctgcacac caaggacaga atccagttca gcttttgaag ccttaccgga ggagataacg
     3181 gtcgcaatgg tacggggaac attaacgtaa tccaccaccc cgaacggtct gtcggggtcg
     3241 agataacgcg gggggatatc tagctgacga cggtagcgaa aaaatccaca tgcagcttaa
     3301 agacctccgc acgcagatta agacgggtaa tgatttcttc aatatcctct tcaacaagcg
     3361 gtctccggat gctgcgtttt ttcggatcgg gaacaaactg cacacagtcc atc
//
LOCUS       DADLTD010000291         3400 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLTD010000291 DADLTD010000000
VERSION     DADLTD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.295"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..2015
                     /locus_tag="JN701_003963"
     CDS             <1..2015
                     /locus_tag="JN701_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY7366231.1"
                     /translation="RGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNVLSK
                     EPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKLKTL
                     RENARTWLLKYPEHAITGLLPSALDKAGEAQDNARAALRMLTENGHQPLLQEIARRYN
                     QPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKANAQSLPDSALLHLGE
                     MLRFPQEEALYPGLLQVKDACTADSLAEFVWDLFTAWLTAGAPSKESWAFTALGVLGN
                     DDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERA
                     KEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFVRDA
                     SGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDARIVAAQQVARQESAMCLRRRWSPE
                     NFKLFLVEHPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYSTADDDLFTLPEGGIS
                     IGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTGAERNASELTRWAGRKC
                     PSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLVMEIDEGFAVGMSPAELSAEQL
                     LSKLWLWEGKAESYGWGSNSTQEAQFSVIDAITASELINDIEALFE"
     gene            2025..3400
                     /locus_tag="JN701_003964"
                     /pseudo
     CDS             2025..3400
                     /locus_tag="JN701_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          884 a          920 c          866 g          730 t
ORIGIN      
        1 agaggggaga ttattctgcg ctgatcagcg aatttaaaac cttctggcac tcctatcaac
       61 gggaatggca gctttacatg ttagccgccc tgcctatcga aaaaacagcg caggcgtgga
      121 atgtcctcag taaagagcca cacgtcggtg tcgagtttgt catgacgcac ctgcaactcg
      181 ctgggttaca gggttttatt cactcctttt cacgctatcc acaagaagct ctacctgtag
      241 cacaatattt tgcggctatc gaattagcgc cactgattgc ccgtgccttc aacaaactca
      301 aaacgctacg cgaaaacgcc cgcacctggc tgttgaaata cccggaacat gccattaccg
      361 gcctgctgcc ttcggcgctc gacaaagccg gtgaagcaca ggataacgcc cgcgctgcct
      421 tgcgtatgct taccgaaaat ggtcatcagc cattactgca agaaatcgcc cggcgttata
      481 accagccgga agtaaccgac gcggtgaacg ctctgcttgc gctcgatccc ttagataatc
      541 acccaacaaa aatccccact cttccggcct tttatcagcc atcgctctgg acgcgcccgg
      601 tattaaaagc aaacgcccaa tcactgccag atagtgccct cctccacctc ggcgaaatgc
      661 tccgcttccc tcaggaagag gctctgtatc cgggattatt gcaggtgaaa gatgcctgta
      721 ccgccgactc actggctgaa tttgtctggg atctgtttac cgcctggctg actgcgggcg
      781 cgccgtcgaa agagagttgg gcgtttactg cgttaggcgt tctcggtaac gatgataccg
      841 ctcgcaaact gacgccatta atccgcgcct ggcctggtga atcccagcat aaacgcgcca
      901 ccgttgggtt ggatattctc gccgctatcg gtagtgatat tgccctgatg cagcttaacg
      961 gcatcgccca gaaacttaaa ttcaaagcat tacaggagcg ggcaaaagaa aaaattgccg
     1021 acattgccga gagccgcgaa ctcacggtgg cggagcttga agatcggtta gcaccggatc
     1081 tcggtctgga tgataacggt tcgttgctgc tggatttcgg cccacgccag ttcaccgtca
     1141 gctttgatga aaccttaaaa ccgtttgtgc gtgacgcttc cggcagccgc ctgaaagacc
     1201 tgcccaaacc aaacaaaagc gatgatgaat cgcggtcgaa cgatgcggtt aaccgctaca
     1261 aattgctgaa aaaagatgcg cgtatcgtcg ccgcccagca ggtagcaagg caggaatccg
     1321 ccatgtgcct gcgtcgccgc tggtcgccag aaaactttaa gctcttcctg gttgagcatc
     1381 cgttggttcg ccacttaacc cgccgtctga tttggggcgt ttatagcgcc gaaaaccagc
     1441 tacaggcgtg ctttagggtg gcggaagata acagctacag caccgctgac gatgatcttt
     1501 tcaccctgcc ggaaggcggt atctctatcg gcattcctca cgttctggaa atatcaccga
     1561 cggatgctgc cgcctttggt cagctttttg ccgactacga actgctacca ccattccgtc
     1621 agcttgaccg taacagttac gccctgacag gagccgagcg caacgccagc gaactgaccc
     1681 gctgggcagg cagaaaatgc ccgagcggtc gggtcatggg gctggcgaat aaaggctgga
     1741 taaagggcac cccgcaggat ggaggctgga tcggctggat gatcaaaccc ctgggccgct
     1801 ggtcgttagt catggaaatc gatgaaggtt ttgcggtagg catgtcgcca gccgaactca
     1861 gcgctgagca gctcttaagc aagctgtggc tatgggaagg caaagcagaa agctatggct
     1921 gggggagtaa ttcaacacag gaagcgcagt tctccgtaat cgatgccatc accgccagcg
     1981 agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac aaggaattac
     2041 cgtggctggc ggataacgcc caactggaac tgaaatataa aaaaagcaaa acgccgctca
     2101 gtcatcgcaa ttggccgggc gaaccggtgt ctgttatcac tgaaagcatc attcagacac
     2161 tgggtgatga attgctacaa aaagctgaga agaaaaaaag attgtctggc gttatgaaaa
     2221 tttttcactg gagtggcagt ccgccatcac gcaggccatc aacttgatcg gcgaacacaa
     2281 accctcagtc ccggcccgga caatggcggc gctagcctgt atcgcgcaaa atgacagcca
     2341 acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg aggtggtaat
     2401 tgcacgcctg tttattgcgc ggtgttatga gagtgatcct ctggtagtga cattgcagta
     2461 tcaggacgag gattatggct atggttatcg ctcagaaacc tataacgaat tcgatctccg
     2521 actgcgtaag catctctctc tggcagagga aagctgctgg cagcgttgcg ccgacaaact
     2581 cattgccgca ctaccaggaa taaccaaagt tcgccgccct tttattgcgc tgatcctccc
     2641 ggaaaaacca gaaatagcca atgagttggt aggccttgaa tgcccgcgaa ctcattttca
     2701 ttctaagaag tggttaaaag ttgttgctaa tgaccccaca gcggtgagaa aactcgaaca
     2761 ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc atgaaaacca
     2821 cttcggctac gcggcctgcg ccgccctttt gcgcgaacaa ggactggcag ccattccgcg
     2881 cctcgcgatg tatgcccata aagaagattg cggcagtctg ctggtacaaa ttaaccatcc
     2941 gcaagtcatc cgcaccttgc tactggtggc tgataaaaac aaacccagcc tgcaacgtgt
     3001 agctaaatac cataaaaact tcccccatgc gacgctcgcc gcactggcag aactgctggc
     3061 gttaacagaa ccaccagccc gccctggtta tccaatcatc gaagacaaaa agctgcctgc
     3121 acagcaaaaa gcacgcgatg aatactggcg tacgctgtta caaacgctga tggcatcgca
     3181 gccacaactg gcagaagagg tgatgcagtg gttaagtact caagccaggg cagtgctgaa
     3241 tagttattta tcggcaccgc ccaaaccggt tattgatagt accgataaca gcaatctgcc
     3301 tgaaatcctc gtttcgctac cgtggcgtag taagaaaaaa atgacagctc cacgtcttga
     3361 tttggcaccg ctcgaattaa ctccgcaagt ttactggcaa
//
LOCUS       DADLTD010000292         3383 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLTD010000292 DADLTD010000000
VERSION     DADLTD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.296"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            186..2102
                     /gene="dnaK"
                     /locus_tag="JN701_003965"
     CDS             186..2102
                     /gene="dnaK"
                     /locus_tag="JN701_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY7366232.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2191..3321
                     /gene="dnaJ"
                     /locus_tag="JN701_003966"
     CDS             2191..3321
                     /gene="dnaJ"
                     /locus_tag="JN701_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY7366233.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHSAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT          913 a          832 c          932 g          706 t
ORIGIN      
        1 ctgcgcaaaa gcacaaaaaa tttttgcatc tcccccttga tgacgtgggt tacgacccca
       61 tttagtagtc aaccgcagtg agtgagtctg caaaaaaatg aaattgggca gttgaaacca
      121 gacatttcgc ccctattaca gactcacaac cacatgatga ccgaatatat agtggagacg
      181 tttagatggg taaaataatt ggtatcgacc tgggtactac caactcttgt gtagcgatta
      241 tggatggcac cactcctcgc gtgctggaga acgccgaagg cgatcgcacc acgccttcta
      301 ttattgccta tacccaggat ggtgaaactc tggttggtca gccggctaaa cgtcaggcag
      361 tgacgaaccc gcaaaacacc ctgtttgcga ttaaacgcct gattggccgc cgcttccagg
      421 acgaagaagt acagcgtgat gtttccatca tgccgttcaa aattattgct gctgataacg
      481 gcgacgcatg ggtcgaagtt aaaggccaga aaatggcacc gccgcagatt tctgctgaag
      541 tgctgaaaaa aatgaagaaa accgctgaag attacttggg tgaaccggta actgaagctg
      601 ttatcaccgt accggcatac tttaacgatg ctcagcgtca ggcaaccaaa gacgcaggcc
      661 gtatcgctgg tctggaagta aaacgtatca tcaacgaacc gaccgcagct gcgctggctt
      721 acggtctgga caaaggtact ggcaaccgta ctatcgcggt ttatgacctg gggggtggta
      781 ctttcgatat ttctattatc gaaatcgacg aagttgatgg cgaaaaaacc ttcgaagttc
      841 tggctaccaa cggtgatacc cacctgggtg gtgaagactt cgacagccgt ctgatcaact
      901 acctggttga agaattcaag aaagatcagg gcattgacct gcgcaacgat ccgctggcaa
      961 tgcagcgcct gaaagaagcg gcagaaaaag cgaaaatcga actgtcttcc gctcagcaga
     1021 ccgacgttaa cctgccgtac atcactgcag atgcgaccgg tccgaaacac atgaacatca
     1081 aagtgactcg tgcgaaactg gaaagcttgg ttgaagatct ggtaaaccgt tccattgagc
     1141 cgctgaaagt tgcactgcag gacgctggcc tgtccgtatc tgatatcgac gacgttatcc
     1201 tcgttggtgg tcagactcgt atgccaatgg ttcagaagaa agttgctgag ttctttggta
     1261 aagagccgcg taaagacgtt aacccggacg aagctgtagc aatcggtgct gctgttcagg
     1321 gtggtgttct gactggtgac gtgaaagacg tactgctgct ggacgttacc ccgctgtctc
     1381 tgggtatcga aaccatgggc ggtgtgatga cgacgctgat cgcgaaaaac accactatcc
     1441 cgaccaagca cagccaggtg ttctctaccg ctgaagacaa ccagtctgcg gtaaccatcc
     1501 atgtgctgca gggtgaacgt aaacgtgcgg ctgataacaa atctctgggt cagttcaacc
     1561 tggatggtat caacccggca ccgcgcggca tgccgcagat cgaagttacc ttcgatatcg
     1621 atgctgacgg tatcctgcac gtttccgcga aagataaaaa cagcggtaaa gagcagaaga
     1681 tcaccatcaa ggcttcttct ggtctgaacg aagatgaaat ccagaaaatg gtacgcgacg
     1741 cagaagctaa cgccgaagct gaccgtaagt ttgaagagct ggtacagact cgcaaccagg
     1801 gcgaccatct gctgcacagc acccgtaagc aggttgaaga agcaggcgac aaactgccgg
     1861 ctgacgacaa aactgctatc gagtctgcac tgactgcact ggaaactgct ctgaaaggtg
     1921 aagacaaagc cgctatcgaa gcgaaaatgc aggagctggc acaggtttcc cagaaactga
     1981 tggaaatcgc ccagcagcaa catgcccagc agcagactgc cggtgctgat gcttctgcaa
     2041 acaacgcgaa agatgacgat gttgtcgacg ctgaatttga agaagtcaaa gacaaaaaat
     2101 aatcgcccta taaacgggta attatactga cacgggcgaa ggggaatttc ctctccgccc
     2161 gtgcattcat ctaggggcaa tttaaaaaag atggctaagc aagattatta cgagatttta
     2221 ggcgtttcca aaacagcgga agagcgtgaa atcaaaaagg cctacaaacg cctggccatg
     2281 aaataccacc cggaccgtaa ccagggtgac aaagaggccg aggcgaaatt taaagagatc
     2341 aaggaagctt atgaagttct gaccgactcg caaaaacgtg cggcatacga tcagtatggt
     2401 catgctgcgt ttgagcaagg cggcatgggc ggcggcggtt ttggcggcgg cgcagacttc
     2461 agcgatattt ttggtgacgt tttcggcgat atttttggcg gcggacgtgg tcgtcaacgt
     2521 gcggcgcgcg gtgctgattt acgctataac atggagctca ccctcgaaga agctgtacgt
     2581 ggcgtgacca aagagatccg cattccgact ctggaagagt gtgacgtttg ccacggtagc
     2641 ggtgcaaaac caggtacaca gccgcagacc tgtccgacct gtcatggttc tggtcaggtg
     2701 cagatgcgtc agggtttctt tgccgtgcag cagacctgtc cacactgtca gggccgcggt
     2761 acgctgatca aagatccgtg caacaaatgt catggtcatg gtcgtgttga gcgcagcaaa
     2821 acgctgtccg ttaaaatccc ggcaggggtg gacactggag accgcatccg tcttgcgggc
     2881 gaaggtgaag cgggccagca tagcgcaccg gcaggcgatc tgtacgttca ggttcaggtt
     2941 aaacagcacc cgattttcga gcgtgaaggc aacaacctgt attgcgaagt cccgatcaac
     3001 ttcgctatgg cggcgctggg tggcgaaatc gaagtaccga cccttgatgg tcgcgtcaaa
     3061 ctgaaagtgc ctggcgaaac ccagaccggt aagcttttcc gtatgcgcgg taaaggcgtc
     3121 aagtctgtcc gcggtggcgc acagggtgat ttgctgtgcc gcgttgtcgt cgaaacaccg
     3181 gtaggcctga acgagaagca gaaacagctg ctgcaagagc tgcaagaaag cttcggtggc
     3241 ccaaccggcg agcacaacag cccgcgctca aagagcttct ttgatggtgt gaagaagttt
     3301 tttgacgacc tgacccgcta acctccccaa aagcctgccc gtgggcaggc ctgggtaaaa
     3361 atagggtgcg ttgaagatat gcg
//
LOCUS       DADLTD010000293         3369 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLTD010000293 DADLTD010000000
VERSION     DADLTD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.297"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN701_003967"
     CDS             complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN701_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY7366234.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYES"
     gene            732..1898
                     /gene="nhaA"
                     /locus_tag="JN701_003968"
     CDS             732..1898
                     /gene="nhaA"
                     /locus_tag="JN701_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY7366235.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1964..2863
                     /gene="nhaR"
                     /locus_tag="JN701_003969"
     CDS             1964..2863
                     /gene="nhaR"
                     /locus_tag="JN701_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY7366236.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2901..>3369)
                     /locus_tag="JN701_003970"
     CDS             complement(2901..>3369)
                     /locus_tag="JN701_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366237.1"
                     /translation="RFNYNLNTILLVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHI
                     RFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDG
                     TNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          818 a          753 c          811 g          987 t
ORIGIN      
        1 gattcgtaag ccgtgaaaac agcaacctcc gtctggccag ttcggatgtg aacctcacag
       61 aggtcttttc tcgttaccag cgccgccact acggcggtga tacagatgac gatcagggcg
      121 acaatcatcg ccttatgctg cttcattgct ctcttctcct tgaccttacg gtcagtaaga
      181 ggcactctac atgtgttcag catatagggg gcctcgggtt gatggtaaaa tatcactcgg
      241 ggcttttctc tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg
      301 ctattatatc gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc
      361 gcatttttgt ggcgtaattt tttaatgatt taattattta actttaattt atctctttat
      421 cgcaattatt gacgacaagc tggattattt ttgaaatatt gtcctaacaa ataacgccga
      481 ctgacaagaa attaattatt acttttccta attaatccct cagaaatcct caccttaagc
      541 tatgattatc tgagcttagg gtcactcgtc agcgcttaca gccgtcaaaa actcatctca
      601 ccgctgatgg cgaaaattct acaaaggctc gtaaaaaacg aattattcct acactataat
      661 ctgattttaa cgatgattcg tgcggggtaa aatagtaaaa acgatttatt cacctgaaag
      721 agaaataaaa agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta
      781 ttcttatcat tgccgctatc ctggcgatga tgatggccaa cagcggcgca accagtggat
      841 ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca
      901 aaaacatgct gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc
      961 tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc
     1021 cagttatcgc cgctattggt gggatgattg tgccggcatt actctatctg gcctttaact
     1081 atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac attgcctttg
     1141 cacttggagt gctggcgctg ttgggaagtc gtgttccgtt agcactgaag atctttttga
     1201 tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta
     1261 atgacttatc gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat
     1321 tgaatctgtg tggtgtacgc cgcacgggcg tctatattct ggttggcgta gtgttgtgga
     1381 ctgcggtgtt gaaatcgggg gttcacgcaa ccttggcggg ggtaattgtc ggcttcttta
     1441 ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttacacc
     1501 cgtgggtggc gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc
     1561 aaggcgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc
     1621 tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg
     1681 cgcatctgcc tgagggaacg acttatcagc aaattatggc ggtggggatc ctgtgcggta
     1741 tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac
     1801 tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat
     1861 acagctggtt acgcgttcgt ttgcgtccat cagtgtgaca ggacggttta ccggggagcc
     1921 ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta
     1981 caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga
     2041 ggcgctttat ttaactccac aaaccattac cggacagatc cgggctctgg aagagcgcct
     2101 gcaaggcaaa ttatttaagc gcaagggacg tggtctcgaa cccagcgagc tgggagaact
     2161 ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa
     2221 ctatcgcaaa gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg
     2281 cctggtcagt agcgtgctga atgctgcagt ggtagaaggc gagccgattc atcttcgctg
     2341 cttcgaatcc acccacgaaa tgctactgga gcaattaagc cagcataaac tggatatgat
     2401 catttccgac tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg
     2461 cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg
     2521 tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct
     2581 taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc
     2641 gttgatgaaa gcttttggtg caatgcacaa tgcaatcttc gttgccccaa cgctttatgc
     2701 atatgacttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga
     2761 agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg
     2821 caatacggat tattctgcgc tttttagtcc agcggcgcgt taatcggcag ctcccccaaa
     2881 gttaaagtgg aggagataga ttagttgtac ataaccacga ttttgactcg gctcattatt
     2941 tgcccgcttg agacattatt tccatatgta cgcgggcgaa taaatagagg aatctgatta
     3001 cttccttcat ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc
     3061 gtaccatcat tggagagttg aaagcctact tgacccgcac cgtcctcact gttttctaac
     3121 tcatttttta atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca
     3181 acatcactgg gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata
     3241 tgatgaaact gataattttc attattgata cgagacagta caaatcgtcc agtgtcgatg
     3301 gattgatctt caaaaccaca ggtcattaaa tttaccagta aaatagtatt taaattataa
     3361 ttaaagcgg
//
LOCUS       DADLTD010000294         3319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLTD010000294 DADLTD010000000
VERSION     DADLTD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.298"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..211)
                     /locus_tag="JN701_003971"
     CDS             complement(<1..211)
                     /locus_tag="JN701_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366238.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVCISLQELQLELMVEYALFF"
     gene            complement(214..981)
                     /locus_tag="JN701_003972"
     CDS             complement(214..981)
                     /locus_tag="JN701_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366239.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(984..1694)
                     /locus_tag="JN701_003973"
                     /pseudo
     CDS             complement(984..1694)
                     /locus_tag="JN701_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1710..3215)
                     /locus_tag="JN701_003974"
                     /pseudo
     CDS             complement(1710..3215)
                     /locus_tag="JN701_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          935 a          797 c          727 g          860 t
ORIGIN      
        1 tgaaaaaaag tgcatattca accattaact caagttgtaa ttcctgaaga gaaatacaca
       61 caaccaggag cggaaaggag aatattattt ctgccagaaa agttaacgtg aatataagta
      121 cggctaagac aataaccgtc ggccatacca ataatggcac gataacaaaa ccgagaatta
      181 cagggggaat ataaagccgg aatctaatca tttttatttt cctagtgatt cggatgatgc
      241 gtcatacatc gcgcataaac actcatcaat tatccagtca tcttcccaga cctgatattc
      301 tgtcattggt tcgggctgtt ttagcgggtt ctcaatcgct tgccgcgctc tttctttcag
      361 taaaggatga ttatttctag cccaggcatt gatttgggtg gtaatgacgt tatcgatatc
      421 gtcctggtta tctctgatat aatcctcaca atatctgttc aataaacagg caagagaaac
      481 ggtcgaccaa aagccgggaa ctgaattaat ggcatccagc aatggcgcaa gagcgatatc
      541 ggcacggaaa aacatttcca gcatatactg taattcgaca gcttcatcgc cggataccca
      601 atcagtcata tactggcagg caaatcgctg taaaattgcc cgttcatccg cacgccagtt
      661 ttctttgttg gcaaggttga tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg
      721 cccctgtgca acaaattcca gtaaacgcgg cagaaaatag cggacctcat cgctattttg
      781 cggatcaggc cctgggctac tattccaggc attgattaag gaatacggga tcgctcgtaa
      841 cgatgtactc cgtaatgcct tttgttcctg ctgactcagg caatattcac aacaaaccac
      901 aaatttctgc ggcacccgat aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg
      961 catggcgggg ctgggttgca tagttacccc ctcgcccgct gatcgcgtgt aaacggttga
     1021 tagacaaaac gagcattcgc cgcgttcagg cgaataaggg cgctacgcga aagggaaaag
     1081 ctggggcgaa tgcaactatt gcagtctcga cagctttcct ctgcgcaatt ttccactgag
     1141 taatgacgcg tgtagagttt cagttcggcc tgcttatcgc gaaatccgcg taaactgtta
     1201 ttgttatgaa atttccaggc atctttaaag acctgaaaga tatagacatc ctcaatccag
     1261 gagtcacatt ttttaagcgg gcgctttgct attatcaccc gctcatccgg atttagcatc
     1321 gccgtaaatt gtacggtgac gtcatttgtc gtgttcaggt cacattgaac atctggtaca
     1381 tcactgatat aggggtcaaa tccaaaatca gcaccacagg cacaaagacg gctaatggtg
     1441 gcggtatcga gggaaaatcc cggccatact ttgctggagg ttaagccaat atttaatgtg
     1501 acatcatatc gaagcaatcc ctcaagaccg tcagcaccga cgaacttttc cccttcatcc
     1561 agggcctagt taataagttg ctgaacaatg ccttcatccc agtatgccga tttgcagttg
     1621 gcaatcgtaa tatccacgcc tgtgaaggga tcagtaccgg caaggcttgc aaataatgta
     1681 ttaactaatt ccatttttat tatccagctt taaggtagcg tcttatacat cgggacataa
     1741 cctacatctt ccaccaaact ctgtccctgc ggcgttaaaa accactccaa caatttttgt
     1801 gtctctgacg tcgtattttc ccgcgttacc ataaaggcat cgatgatata gggatatttg
     1861 ccgttgcgaa tgttttcggc agtcggtgca ataccgttaa tcgctagcag ttttatattt
     1921 ttatcggcgt tcatttgtgt tgcgtaataa cggaaggtgt aacctattgc gttgtttgta
     1981 ttacggtatt cggcaacgac tttaatcatt ccctccatca tacttgccac ttctgtttcc
     2041 tgcggcgaga tcatgcggac atttttcatg acctgggatt gcatcaccgt ctggctacca
     2101 gagtcttccg ggcgttgcta ggtctggatc tcctgatcgt taccgccaac agtacgccag
     2161 ttggtaattg caccactgaa gatgtcacgc acctgttgtt cagtcaggga attaaccgga
     2221 ttatccgcat tgacgatgaa aacaaacgct tcacgggcaa aaggcgtgta cagcaaagta
     2281 atgcccgatt cctcagcgcg ttttttctgc ccaccggaag gttgcgccac aaaaataata
     2341 tcagcatcac ctttaacaat cctgttatac gcatcggggg tacgagagct ctccagatat
     2401 tccctggtgt gaaaatcttc cggtataacg ctcaaagcat aaaatgcaga ggcataaatt
     2461 gggtacgccg ccgtagcacc atcgagtcgc ggccagtttt gcgtaaactg aatttgcggt
     2521 ttaccacgca gtggtgtcag ttgattattg agtttatcgg gtcgccaggc ccaggtatca
     2581 atattttcgc gcacaccgac gccgggattg agctgtttat ataaataacc ctgataacca
     2641 gcgacaaaac caagcatgag aaggataaac aggagggaat tacggcaacg tagcccggat
     2701 gtaccggtta aaggatgtct ggttggccgg ccgtacccca gcgaaaaact gatatgcgcg
     2761 ccaacgggta taatgactat ccaccagtac tcacctccga ctactgagac taacaaaacg
     2821 cccagccagg ggagcataag atcagccaac caacccgatc ccatctggtg aaattgataa
     2881 tctgccagat gcagcgcgat tatccagcca accaggatta ataaaccggg tactaaaaaa
     2941 agacattgtc gccagacggc tatttctctt ttttctggtg cactatcgcc tgcccatact
     3001 cgccccgaga aaaatagcat taatgcgccc agcgccagca taaaaatgtt gagcagaagg
     3061 taacttgttg gcgtgataag gatattacaa acttcaataa taccggcaag tccgacaccc
     3121 agcatagcaa caaccgccgc caaaaattgc gtcagtatgg ggatgccgaa aaaagtcatt
     3181 accagcgagg acaaaatcca ttttctgttt tgcattattc tttccattct ttttgaatgg
     3241 tgaaattata ctccccgagt ccccttgccc cttctggaca cttttccgaa atgatggcgg
     3301 aaaaaaacgg gaccctttg
//
LOCUS       DADLTD010000295         3227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLTD010000295 DADLTD010000000
VERSION     DADLTD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.299"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1010)
                     /locus_tag="JN701_003975"
     CDS             complement(<1..1010)
                     /locus_tag="JN701_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7366240.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCQLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NG"
     gene            complement(1272..2862)
                     /locus_tag="JN701_003976"
                     /pseudo
     CDS             complement(1272..2862)
                     /locus_tag="JN701_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521525.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT         1034 a          592 c          507 g         1094 t
ORIGIN      
        1 ttaccattgc aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc
       61 attatgcgtt ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg
      121 ccggtttggt cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa
      181 ggctgaagac tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata
      241 ttagtgtcaa gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct
      301 atagtaagca actggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta
      361 gcaaaaagcg cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc
      421 ctggtacttg tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg
      481 gggtttataa ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc
      541 cagatgggtt tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata
      601 ttattaagta aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg
      661 taatttttac tattaattgt cacaataaac tgataattat tatccctacg tagaatatga
      721 gcacgcatgc ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa
      781 attttgtcat cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc
      841 acaacctcac cgagcattgt cctggcaata cttgatatat catcacctat accatttgca
      901 atatacccaa acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt
      961 gcgattgtcg ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc
     1021 atattattca ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt
     1081 tcgatggaaa aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat
     1141 ttcttatttc atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct
     1201 ctacaatcga ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca
     1261 ctcatggagt tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca
     1321 cgtccgttag gagctatctt ccaggaattt agccccatgg tataattatc aattttttga
     1381 tgctgatcta cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt
     1441 ccacatttga agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg
     1501 tcatatacag gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc
     1561 atgtgttgtt tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct
     1621 ttcatctgat taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt
     1681 cttattattc cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg
     1741 agcgttttaa ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca
     1801 gtatagttac cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt
     1861 atacgactat ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat
     1921 ttatcaccaa aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg
     1981 gtaatcgcat cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc
     2041 gtatctaata aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg
     2101 tcttcgtgtt ttaataattc acataataaa ctccacccct tttccctgat acctatatgt
     2161 cctgaaacga gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa
     2221 gccgtgttgg gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa
     2281 aacacagaat taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc
     2341 caacgctagt cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata
     2401 gaaaggccat taactttata aattatgtct gatttatctc cggagaaact aataaaataa
     2461 taaccatcct tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta
     2521 ttgtctggta taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat
     2581 tcttgaatat ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact
     2641 atattcattt accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc
     2701 gttatctatt tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt
     2761 tttatcaacc ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg
     2821 gcttgaagag atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt
     2881 ataacttgtt agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca
     2941 aagtaataat acttatatat ccgatgattt taagcaccga gactatccgg tcttttacta
     3001 aataatggaa aacaatccat ccctggaaga taattgaaaa aaacaacaga ataagaatta
     3061 atcttaggat aaacatttat ttatcatggc cttttgaacg ctgaaaagta gcgctctgta
     3121 aagtaaaccc cgctatagtt atgctatgca tttgtcttta atgatatctc gtcattgtat
     3181 taacaggctg aaattatcat taatgcacat ttcaatatta gcaggga
//
LOCUS       DADLTD010000296         3214 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLTD010000296 DADLTD010000000
VERSION     DADLTD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.300"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..907
                     /locus_tag="JN701_003977"
     CDS             <1..907
                     /locus_tag="JN701_003977"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY7366241.1"
                     /translation="ANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNK
                     AWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDM
                     NISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCV
                     KVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVY
                     YSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALS
                     CEYTNNPDLSIANVISLVENAERLN"
     gene            874..1833
                     /locus_tag="JN701_003978"
                     /pseudo
     CDS             874..1833
                     /locus_tag="JN701_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
     gene            1927..3167
                     /locus_tag="JN701_003979"
                     /pseudo
     CDS             1927..3167
                     /locus_tag="JN701_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
BASE COUNT          769 a          721 c          869 g          855 t
ORIGIN      
        1 ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg attcaaatcc
       61 aggtaaaata aaatttacgc ctggtggagt cgggcgcaat attgcacaaa acctggcgtt
      121 gctggggaac aaagcctggc tactgagcgc cgtaggcagt gacttttatg gtcaatcgct
      181 gctaacgcaa accaatcaat ctggcgttta tgtcgataaa tgcctgattg tgccgggaga
      241 aaatacgtct agttatttat cattactcga taataccggt gaaatgctgg ttgctataaa
      301 tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc gtgaatttat
      361 tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac tggcatggat
      421 tctggataat gccgccaacg tacccgtatt tgtcgatccg gtttccgcat ggaaatgtgt
      481 caaagtgcgc gaccgtctaa atcagatcca cactctgaag ccaaaccgcc ttgaagcgga
      541 aaccctgagt gggattgcgc tgtcagggcg tgaagatgtg gcaaaagttg ctgcctggtt
      601 ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg tttattacag
      661 cgatatcagc ggtgaaaata gctggtctgc gccgatcaaa accaatgtta ttaatgttac
      721 cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg gaatgccgtt
      781 tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct gtgaatacac
      841 caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaacg cagaacgtct
      901 gaattaaaaa tttcccctga attattacaa atttccccgg aagtgcagga cgctttaaaa
      961 aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat gccgttccca
     1021 caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg cgctgtacct
     1081 gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga agaaattgaa
     1141 ttactgggtc gtgaagggca taacgtgacc aaagttagtc gtcgcgattt accttttgtt
     1201 gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat tgcggcgctt
     1261 gccggaatta aagtatttgc caccggggga attggtggtg tgcatcgcgg ggcggaacat
     1321 accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac cgttgtttgt
     1381 gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga aaccttcggt
     1441 gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt ttttctgccg taccagctcg
     1501 tttgacgtca gtattcgtct cgacagcgcc agtgaaattg cccgtgcaat ggcggtgaaa
     1561 tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga acagtttgcg
     1621 atgccggagg aatctatcaa tgcagccata gattaagccg tcgccgaagc cgaagagcag
     1681 ggcgttattg gtaaagaaag tacactgttc ctgctggcgc gcgttgctga gctgaccggc
     1741 ggtgacagcc tgaaatccaa tatccagctg gtattcaaca acgccattct ggcgagtgaa
     1801 attgccaaag aataccagcg tctcgcgggt taatttccga cgggcagaaa gccgtcgccc
     1861 gtactcatca agcataccaa aacctggcgc tgacgccggg tttactggca tgaagggaaa
     1921 atcattatgg atataatgag aagtgttgtg gggatggtgg tgttactggc aatagcattt
     1981 ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc actgctgctg
     2041 caaatcgcta ttggtggcat catgctctac ttcccaccgg gaaagtgggc agtagaacag
     2101 gcggcattag gcgttcataa agtgatgtct taggtagcgc cttcattttt ggttcgctgg
     2161 ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt tatcttcgcc tttcgcgtac
     2221 ttccggcgat tattttcgtt actgcgctca tcagtctgct gtactacatt ggcgtgatgg
     2281 ggctgctgat tcgcatcctt ggcagcattt tccagaaagc tcttaacatc agcaaaatcg
     2341 aatcttttgt cgcagtcaca actattttcc tcgggcaaaa tgagatcccg gcgatcgtta
     2401 aaccgtttat cgatcgcatg aatcgcaacg agttgtttac cgcaatttgt agcgggatgg
     2461 cgtccattgc tggttcgatg atgattggtt atgccggaat gggcgtacca attgactacc
     2521 tgttagcggc atcgctgatg gcgatcccag gtggtatttt gtttgcacgt attcttagcc
     2581 cggccaccga gccttcgcag gtcacatttg aaaatctgtc gttcagcgaa acgccgccaa
     2641 aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg gctgaaaatc gccgctggtg
     2701 tagcgacggt ggtaatggca tttgtcgcaa ttattgcgct gatcaacggc attatcggcg
     2761 gaattggtgg ctggtttggt ttcgccaatg cctctctgga aagtattttt ggctatgtgc
     2821 tggcaccgct ggcgtggatc atgggtgtgg actggagtga tgccaatctt gcgggtagcc
     2881 tgattgggca gaaactggcg attaacgaat tcgtcgctta cctgagtttc tccccatacc
     2941 tgcaaacggg cggcacgctg gaagtgaaaa ccattgcgat tatctccttt gcgctttgtg
     3001 gttttgctaa ctttggttct atcggtgttg tcgttggcgc attttcggct atttcgccaa
     3061 aacgcgcgcc ggaaatcgcc cagcttggtt tacgggcgct ggcagcagca acgctttcca
     3121 acctgatgag tgcgactatt gccgggttct ttattggttt agcttgagtt tcattgccgg
     3181 atgtacgcgc ttattaagcg ttgcatccgg caat
//
LOCUS       DADLTD010000297         3200 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.301, whole genome shotgun sequence.
ACCESSION   DADLTD010000297 DADLTD010000000
VERSION     DADLTD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.301"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..2868
                     /locus_tag="JN701_003980"
     rRNA            <1..2868
                     /locus_tag="JN701_003980"
                     /product="23S ribosomal RNA"
     gene            2946..3061
                     /gene="rrf"
                     /locus_tag="JN701_003981"
     rRNA            2946..3061
                     /gene="rrf"
                     /locus_tag="JN701_003981"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          843 a          715 c          986 g          656 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accattataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tattgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctg atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa actaaggctg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatat gtaggtgaag tccctcgcgg
     1681 atggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt cagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgttg acccgtaatc
     2161 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
     2221 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
     2281 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
     2341 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
     2401 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
     2461 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     2521 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     2581 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     2641 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     2701 ctaagcacga aacttgcccc gagatgagtt ctccctgact ccttgagagt cctgaaggaa
     2761 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     2821 cggtactaat gaaccgtgag gcttaacctt acaacgccga aggtgttttg gcggatgaga
     2881 gaaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2941 atttgcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     3001 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     3061 catcaaatta agcgaaaggc catccgtcag gatggccttt ttgcattggc gcagaaaaaa
     3121 tgcctgatgc gacgctgcgc gtcttatact cccacatatg ccagattcag caacggatgc
     3181 ggtttcccca acttgcctac
//
LOCUS       DADLTD010000298         3195 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.302, whole genome shotgun sequence.
ACCESSION   DADLTD010000298 DADLTD010000000
VERSION     DADLTD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.302"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            199..939
                     /locus_tag="JN701_003982"
     CDS             199..939
                     /locus_tag="JN701_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY7366242.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKLGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(910..1677)
                     /locus_tag="JN701_003983"
     CDS             complement(910..1677)
                     /locus_tag="JN701_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY7366243.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="JN701_003984"
     CDS             complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="JN701_003984"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY7366244.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2701..>3195
                     /locus_tag="JN701_003985"
     CDS             2701..>3195
                     /locus_tag="JN701_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7366245.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFF"
BASE COUNT          765 a          809 c          819 g          802 t
ORIGIN      
        1 atcttaggaa ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa
       61 atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt
      121 ccaaatccag actattccga tttgggatcc attttgccgt cctggcgtgt gtaaccgttt
      181 tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg
      241 tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag
      301 tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg
      361 ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt
      421 aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg
      481 ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat
      541 attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc
      601 cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc
      661 ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac
      721 ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc
      781 tgggagcaac tgaagctggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt
      841 tctgtggtca atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca
      901 caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat
      961 cttttgccag gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt
     1021 cggtgtggtc tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc
     1081 aaacggtgcg cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg
     1141 cccatccgaa agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc
     1201 gatatattta aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt
     1261 atgcagcagc cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc
     1321 ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc
     1381 ccataactcg cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg
     1441 gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat
     1501 gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa
     1561 ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt
     1621 gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg
     1681 taacctccac aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag
     1741 gtcggatgcg gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg
     1801 ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg
     1861 ccttatccgg cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga
     1921 tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc
     1981 gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca
     2041 ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg
     2101 aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga
     2161 aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag
     2221 caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt
     2281 gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa
     2341 agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta
     2401 aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcttggtaca
     2461 tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa
     2521 agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa
     2581 cagcaacaat gtgagctttg ttgtaattat attgtaaata cattgctaaa tgtatttaca
     2641 tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt
     2701 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc
     2761 gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct
     2821 ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt
     2881 gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg
     2941 ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc
     3001 gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg
     3061 accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat
     3121 gatttccaga ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa
     3181 gagcatcgct tcttc
//
LOCUS       DADLTD010000299         3172 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.303, whole genome shotgun sequence.
ACCESSION   DADLTD010000299 DADLTD010000000
VERSION     DADLTD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.303"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..512)
                     /locus_tag="JN701_003986"
                     /pseudo
     CDS             complement(1..512)
                     /locus_tag="JN701_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            complement(758..1210)
                     /locus_tag="JN701_003987"
     CDS             complement(758..1210)
                     /locus_tag="JN701_003987"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7366246.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(1207..2271)
                     /gene="dinB"
                     /locus_tag="JN701_003988"
     CDS             complement(1207..2271)
                     /gene="dinB"
                     /locus_tag="JN701_003988"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY7366247.1"
                     /translation="MYKKVKIIHVDMDCFFASVEMRDNPALRDIPIAIGGSRERRGVI
                     STANYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEP
                     LSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNK
                     PNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRF
                     GKFGRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERR
                     LAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRL
                     VGLHVTLLDPQMERQLVLGL"
     gene            complement(2318..3088)
                     /locus_tag="JN701_003989"
     CDS             complement(2318..3088)
                     /locus_tag="JN701_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366248.1"
                     /translation="MIYSNQLRYTGKDGVYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGESGSILISRDTKLVENFIDTLENWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSV
                     KNGESTFTASANEQASKVLKSGNALRKYSVNPNKKGTY"
BASE COUNT          832 a          761 c          712 g          867 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcta gtgcaaacag
       61 ttcagcgttg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcacc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccagatc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccata ccgcagccga tatcattgcc
      301 gaccagtgcc ggataaaaag accaacagag aagaacgctg cgccaatcgg atagccgcgt
      361 ccgggatgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
      421 tgtaattgtt ggatcgctaa actctcgatc cactggtcat cagatgcgat ggtaaatacc
      481 gcatcggata agggacgaat ataattgccc atataccatt ccgtaaatag actcgtcata
      541 cttcaagttg catgtgttgc gacttgaatt attgagagta acaattaaaa aatcaggaaa
      601 tggcagacga atccagggca agaaaaaaat aattaatcct ggaaaggtct gcaaagagtc
      661 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
      721 catactaggc ttgcgttatt ttcattgcaa gctggattta atgttgctgt ttataacgca
      781 tataaaaatt aataaaccac tctccccgac attcaacgcg ttgctgctta actgtctgaa
      841 aaccataacg ttcaaaaaag ggttttgcgg ttatgcttgc gtccaccgta agttcggatt
      901 cagacttaat caaaggtttt aacaaagcgc tggcaacccc acggcgggtg tattcagggt
      961 caacaaataa catatcgata tagtgttcaa cgcaggtaat aaagccgacc agttttgcat
     1021 taatgactgc aacccgcact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
     1081 tctgcgccca ggcggcaatt tgttgtggtg aataatgctg actggcagtc atcgtaaccg
     1141 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagtct cttatttgta
     1201 tgttattcat aatcccagca cgagttgtct ttccatttgc gggtcaagca acgtcacatg
     1261 cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaagttt tacgcgcggt
     1321 ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgagtgg tttgctgaaa
     1381 atcatcaaac tttaatttca ccccctggcg agcaatcagt aaatcaggtt tcacctttgc
     1441 cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt cagaccagtg
     1501 gtggatatct tccgccatcg tgcgttccac gccgacggat tttcgcaacc gttcgctgtt
     1561 aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa atttgccaaa
     1621 gcgtttaagc agcatcacca gatcacactt ttgtacatca ccgcaggttc gtagccccat
     1681 cgcttccagt tttgccgccg agactttgcc gacgccgggg atttttgcca gtggtaaggt
     1741 ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tggccgttgg gtttattcat
     1801 gtcggaggcg attttggcga gaaactttac gggtgccacg cccgcagacg ccgtcagttg
     1861 cagctcgttg aaaatcgtct ggcggatttc ctgggcgatg agggtcgcag aaccgtggca
     1921 atggacgcta tcggtgacgt cgagataagc ctcatccagt gacaacggtt caatacgcga
     1981 ggtgtagcgc gagaagattt cgcggatatg atttgaggct tctttgtagg cgtcaaagcg
     2041 ccccggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg gcatagcgct
     2101 acgtacgcca aatttacgcg cgggataatt ggcggtactg atcacccccc gacgttcgcg
     2161 gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca tctccaccga
     2221 cgcgaaaaag cagtccatat ccacatgaat aatcttcact ttcttataca ccctgtatat
     2281 ttatacatgc aaagagatta tacacacaaa agcaaaatca ataagtgccc ttcttattcg
     2341 gattcacaga gtacttccgt aaagcattac ccgacttaag cacctttgaa gcttgctcat
     2401 tagctgaggc tgtgaaggtg ctctcaccat ttttaacgct aacgctctta gagcacgtta
     2461 cacgggcttt tagcgcttca tctgataact cacttgccga atagctaaaa agctctttac
     2521 aggtcagcat agggcttcca aggctcgttg tagtgttgcc agtattcttt ttactggtag
     2581 caccaccatc attcgtggta gcagtcttgc cagttgcagc cgaaatatca gtctgaccca
     2641 caatagcttt gaagttaatt tcctgaaaag ttagctggat tcttagacca tttgaaatac
     2701 caacatcttt agaggcttta aagctcgtga taatggaatc atcaatttta attccgtctt
     2761 tacagatgac cgaaataatc tgcttctggt cacgccagtt ttccagagtg tcgatgaagt
     2821 tctccaccag cttagtatct cgtgagataa ggatactacc ggattcacca actacaacga
     2881 caccactaat agtgattgtt ctgggtgctc tttgcacatt atctgtgacg gtttgccctg
     2941 attgcatgtt ctgtgtagtc acctgcatag ggctgtcaaa ttccatgttt tcagttgctg
     3001 ataaggttaa gaaggcatct acattatctc ttaagtggaa gtaaacgcca tctttgccag
     3061 tgtaacgaag ttgattagag taaatcatta aattctcctt tgatttcaaa ggcttagtgt
     3121 attttaatca tttttaacca aacctttgat atataagtat tttctctgtt aa
//
LOCUS       DADLTD010000300         3172 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLTD010000300 DADLTD010000000
VERSION     DADLTD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.304"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            345..504
                     /locus_tag="JN701_003990"
                     /pseudo
     CDS             345..504
                     /locus_tag="JN701_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(722..2812)
                     /gene="iha"
                     /locus_tag="JN701_003991"
     CDS             complement(722..2812)
                     /gene="iha"
                     /locus_tag="JN701_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HAY7366249.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          795 a          836 c          732 g          809 t
ORIGIN      
        1 gacctctcag taaatatcaa tacggttctg gcgagccgct tacgtgcagc agtcttatcc
       61 ggcagtttaa aggggaggac ggagaccaat gaggcactgg agcagattca gtgcagaggt
      121 gacctgcttt atcaaactta attgtgacaa aatggcgatt gagaaggtgc aattgactta
      181 tccggattcg gtcgtggcga ggtggtgccc ctgcattctg ctaaccgggt tatgagccag
      241 gagtcagcga tagacaaagg gacttatctg accgggcccc cggaaaaatg gcgggagaga
      301 atgatacctt cctgctgacg agaccggact ttgagatgac gagcgatagt gcggcagtga
      361 tgtacagcct gatcggtagc tgcaaaactt aacggaatcg agccggaaac gtggctacgc
      421 cacgtgatca gtgtaatcaa cacctggcct gccaaccgca tgaaagagtt gttgccctgg
      481 aacgtcactc tctctgtaaa ctaattttta ccccacgtcc ttcacggtgc gcttacactc
      541 tggacaagta tgtaaatgat aagttaaaaa taatccgtgc atttcctgtg acgatattag
      601 ttgttggtaa cgaaatctca ttagcggatc ggaaatgcac ttaacctaat acgttgccac
      661 tgttccgcca ggagttgcag tggcaacgta ttctaccgtc agtgatagcg ttttgttatt
      721 atcagaactg atagttcagc gacatccagt aatttcgctc aggtatcaca tatcctgttg
      781 ttgatgatcc cgtctggaag taatcaccgg catacagcgt actcttaccg gcactgtaca
      841 ggctcacgtc actgtaatcc ttgttgagca ggttattcac cgcagcattc agcgtcaggg
      901 catccgtcat cttccaggac agacctgcat ccaccaccgt ccaggctttc aggtattctc
      961 ctttctcatc atacactttc ttctgtacag cgctcagtga cgaataattc tgggtgaaac
     1021 gtggtgtttt cccgcgataa cgggcaccca gccatgatgc cacctcttcg gtgatctgcc
     1081 agttcagttt cgcattcacc atgtgttcag gggtataact cagcggcgca cctttgttat
     1141 caccatcacg ttgttcactt cgggtccagg tgtaattcag tgacagcgtg acatcctctg
     1201 accacagcgg caatgtgccg gcaaattcca caccgtgcaa ccgggccttt ccgctgttta
     1261 catagctatt ggtgttatca tttatggaat aagagacaat cttgttggag aagtcagtca
     1321 taaaacctgt gacattggca ttcagaccgg cggggttatc gtaatacacc ccagcctcat
     1381 aactgacgct ctcttccggc ttcaggtcgg ggttaccaag tagatttgtt tttccctgcc
     1441 cggacacacc actaatccct ttatgtagct gccccattct gggtgcctta tatcccgtgg
     1501 tcacaccgcc tttcagcgtc caggcatctg ccacatccca gaccagatat gcacgcggac
     1561 tgaagtgtcc cccgaattgc tcatgatgtt catagcggct gcccgcagtc agcgcaagtg
     1621 catccgtgag atgccactca tcctcagcaa ataccgacca gcttttctgc cggaaagttt
     1681 cacctgtgct ggcaaggaca actccgtctt tcatggacga gctctgaaac tcgcccccca
     1741 ccgtaaccag atgagattct cccagagggg taagcagtaa tgaattcagg ataaggttcg
     1801 attccttaag ctcccgcggc tgaccggcaa gcccccattt gtcgcgcttc agtacactgc
     1861 gtacgagctc acgaccttta ttttctgtct cgttccagtt cagatacgat ttccatgttc
     1921 cgaaggtgaa agtatgatca tagccagctg aaattttgtt ccgctcatag cgcagggtcc
     1981 ggtcatatcc ccccgtcaga ctccccagtt gcccatcccg gttatcataa cgctgccggg
     2041 tggtatccat atcaaaccag agcacatcct gctccgacgc cttccagtca agacgtgcac
     2101 caagattata attctgtgac tccgtgggat aaggaatacg cgtggctgct gtatcgctca
     2161 gtgatgtgac cgatgagccc tgacgctgtt gtgtgctacc gcgtacctgc aggctgacag
     2221 aatcatccac aagggggcca ctgctccaga aattaaactg gctgctgtta ccccatttgt
     2281 tgctttcctg cagattcagc cctgcattga cggaagagag ccatttgtct gcattctttc
     2341 tggtaatgat attcaccaca ccgcccatcg catcagagcc atacagtgtg gacatcggcc
     2401 ccctgataac ctcaatacgc tcaatggcgg ccagaggggg catgaacccg gtattcatgg
     2461 cagaaaaacc gttgggagtc acgtcactgc ttccgccctg acgaacacca tcaatcagta
     2521 tcagcgtgta actggctggc attcctcgga tgctgatttc cagcccaccg gttttacccg
     2581 taccactttc aacatccaca ccctctactg atctcagagc ctccgccaga tcgtggtact
     2641 ggctggactg caattcctcc tggctaatca cagaaacact ggcggctgcg ttagtcagct
     2701 ttttctcata gccggatgcc gagacaatca tcacatcctc tgcagcagct atgctgctgg
     2761 ctgaaaatcc gagacaggga atgactacgg aagccagagt ggttattcgc atattactaa
     2821 tctccgatca tgttaaccgg caagacaata cacaaataca aacaataatg agaataattt
     2881 atatttgcct tttacaaaaa ataaagatta ttaacaaaac taaatataaa taacaactgc
     2941 ctcggcatgc cagttaacag ctatccgtat ggcgtgttcg gttttgcgaa taaaaaatgg
     3001 ttactgtaga agcggatttc caccacatgt caaccatgaa tggtcagtgg tgcgagacga
     3061 ctgggatgat ttggtcgatg acttcttccg gtgaatactg atagaaacat tgaaatattg
     3121 taagagaaag cgttcaaaat tctgtggcac gtgaaaaatt ctacagcgtc at
//
LOCUS       DADLTD010000301         3102 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLTD010000301 DADLTD010000000
VERSION     DADLTD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.305"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..329)
                     /locus_tag="JN701_003992"
     CDS             complement(<1..329)
                     /locus_tag="JN701_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366250.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGF"
     gene            complement(395..739)
                     /locus_tag="JN701_003993"
     CDS             complement(395..739)
                     /locus_tag="JN701_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HAY7366251.1"
                     /translation="MTEADLYPHLAHLAGGQVSPYVAPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(736..1182)
                     /locus_tag="JN701_003994"
     CDS             complement(736..1182)
                     /locus_tag="JN701_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAY7366252.1"
                     /translation="MIEILLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRTPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(1179..1499)
                     /locus_tag="JN701_003995"
     CDS             complement(1179..1499)
                     /locus_tag="JN701_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7366253.1"
                     /translation="MISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGA
                     EMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRL
                     GGEK"
     gene            complement(1539..1865)
                     /locus_tag="JN701_003996"
     CDS             complement(1539..1865)
                     /locus_tag="JN701_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7366254.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVLREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1862..2461)
                     /locus_tag="JN701_003997"
     CDS             complement(1862..2461)
                     /locus_tag="JN701_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAY7366255.1"
                     /translation="MPAGKSLTPDDVRPMLEQMVTEAVSHIPVPRDGRDYDPDVLQKA
                     VLDAVSALPAPQDGRDATALEILPAIDVQKSFPRGTYATHQGGLWRAYEKTHGMRGWE
                     CLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTV
                     TWGGSLWHCNSMTEDKPREAHSSAWTLAAKRGRDAGGGK"
     gene            complement(2401..3066)
                     /locus_tag="JN701_003998"
     CDS             complement(2401..3066)
                     /locus_tag="JN701_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HAY7366256.1"
                     /translation="MTVSVNNYGVSDTKQVTLIADAGTAKLASLTSVYSFVVSTTEGA
                     TMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDRGFAEILVTSTEVGLKTVSAS
                     LADKPTEVISRLLNASADVNSATITSLEIPEGQVMVAQDVAVKAHVNDQFGNPVAHQP
                     VTFSAEPSSQMIISQNTVSTNTQGVAEVTMTPERNGSYMVKASLPVKVSPRMMCVRCL
                     NRW"
BASE COUNT          671 a          969 c          770 g          692 t
ORIGIN      
        1 ttaaagccct taccggacgg aaacagcacc ttaaacgcat acaacgcgtc attgtcatag
       61 gcatcacgca gggcgttctg ggcctgattc agataaaaat tacccgacat ggaaatctca
      121 gacgacgccc ccagaccgtt gatgttctcc tgctctgtgg agcagagcgt ggtcacatca
      181 atatcctgtt tctgaccggc ggtgaactgg acttccttga tggtgcagtc caggcgcaga
      241 tattccgcct tatccatagt ttcagcagtc gccggggcag atgaaatcat cacctgcgtc
      301 agctgtgagc gttcatacaa agcagacatt ctgcctcctg ataataaaaa acccgcacgc
      361 ggcggggtat ggttttgtag aaaaaaagaa aaagtcacac cgtgacctga aactccaggg
      421 ttgcacggta acagcggttt tccggaatat agtcctgcat ttcactgacg gatcccgggg
      481 ccagcagcat tatggcttca cgggcgtcct gacgtatctg acgcgcctgc gtcacagtcc
      541 cggcataaac gtctatctgc accgacactg aggactccgc ctgcccgccc atcacgtccg
      601 ctgacaccga tgaaatcagg ctgaaaacca cccacggaag cgccaccgac ggcctgccat
      661 ccagcagggg ggccacatac ggggacacct gcccgccggc aagatgcgcc agatgaggat
      721 acaaatccgc ctccgtcatc gtctcagtac ctcatcaatg gcccggttca tccgcgcaat
      781 cgccacctga gctgcctgtt cactgcgcac atcaaacgcc gggcgcacaa acgggtgcgg
      841 tggcatattc acggtcccca tttccacaaa ccgccagtag aaagcattgc gcgggttatc
      901 cgccttcatg gtgttatcgc tgttaccggt gtccggatta acaccacgga tatggacacc
      961 ggattccatc ccgccatcgc gggagcgccg ggaaaggatc accacattgc ggcgcagttt
     1021 tcccctgcgt accggtgtcc gtgacaccac ttcttctttc agcacattcg cacccgcacg
     1081 ggttgcctca cgtagcaccc ggttattttc cgcaccactc agaagctgca aatcgcggct
     1141 gatgtcctcc agccccgaaa aatccagcag gatttcgatc atttttcccc tcccagccga
     1201 cagagaattt ccagacgccc gccggtcgca tccggcacgg gcagcccgac aacgttcagg
     1261 atccggtcac gccatggacc actcagcaca tgaagtcgtg acgctgccgt gatttcccga
     1321 ccggactgac cgcgcaccca gatgcggatt tccgcctgcg ccatttccgc accggactgc
     1381 atccgctccc ggctgctcct gcctcggata tccgcatgaa ttttcccgca tgacacccat
     1441 tcttccgtca tttctccggc agcgttacgg gttaacaccg ggttcagaac acttatcatc
     1501 tatgtcagac gacctgcaga tattgccatt ccccctcctc ataacaccgt cggacaacgc
     1561 aaatcgtaaa ttagcacgga aacagaaaac ggcagctccc cctgaagcag ttcttcccgc
     1621 tccgcaagat ccggattccg gtacagcatc ccggtcagtc gcatggcagc ccccttcatc
     1681 cgggttaatg cctcgcccgg gatcagttca ccgtcctcac ggagcacttt atcccggctg
     1741 ccctgaatgt aggccagcag cacggcggta gcctgacgaa ccttgtccat cagcatgtca
     1801 tcatccgcgt catggtcgac acgcagatgt gccttgatct cttccagtgt cagtaatgcc
     1861 gtcattttcc gcctcctgca tcccgcccac gttttgcagc cagggtccag gctgatgaat
     1921 gagcttctcg gggtttatct tcggtcatac tgttgcagtg ccacagcgag cccccccacg
     1981 tcaccgtatc gccggggtgg taggtttcac cggctctgaa cacaccgcgg tagagcatca
     2041 ccggcaggga aaatgttttt tccgtacgct ggccactgct ctgccggacc accacagaga
     2101 acaaccgctc acccgtcatg ctgacgtcaa tatccgccac cccgtcaacc aggcattccc
     2161 atccccgcat cccgtgcgtt ttttcatacg cccgccagag tccgccctgg tgtgtggcat
     2221 acgtgccccg gggaaaggat ttttgaacgt caatggcggg gagtatttcc agagccgtgg
     2281 catcacgccc gtcctgcgga gccggcaggg cactcaccgc atccagaacc gccttctgca
     2341 gaacatccgg atcgtagtca cgaccatcac gcggaacagg gatatggctt acagcctccg
     2401 tcaccatctg ttcaagcatc ggacgcacat catccggggt gagactttta ccggcaggga
     2461 tgctttcacc atatacgaac cgtttctttc gggcgtcatg gtgacctcgg ctacaccctg
     2521 cgtattagta gagaccgtat tctggctgat gatcatttgc gaggatggct ctgcactgaa
     2581 tgtcacgggt tgatgcgcaa ccgggttgcc aaactggtcg ttaacgtgag ctttaactgc
     2641 tacgtcttgt gcgaccatta cctgaccttc cggtatctcc agactggtaa tcgtcgcaga
     2701 attaacatct gcactggcat tcagtaatcg cgagatgact tcagtaggtt tatctgccag
     2761 agaggctgaa actgttttca gtccgacctc ggtgcttgtc acaagaattt cagcgaaacc
     2821 ccgatcatcc gtttcaacgc tggtgctgct tagcgtgacg gaggttccgc ggaaattaac
     2881 ttttatgcct tctaccgggt tgccgttagc gtcagtgacg cttgccgtca tggttgcgcc
     2941 ctcggtcgtg ctgacgacga atgagtatac agaggttaag gaggctagtt ttgcggtacc
     3001 agcatcggca atcaaagtca cctgtttcgt atcactgaca ccataattgt tcacgctaac
     3061 tgtcacgggg taggtgccgg attttgtacc gctcagtgta ac
//
LOCUS       DADLTD010000302         3097 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLTD010000302 DADLTD010000000
VERSION     DADLTD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.306"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..923)
                     /gene="curA"
                     /locus_tag="JN701_003999"
     CDS             complement(<1..923)
                     /gene="curA"
                     /locus_tag="JN701_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY7366257.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDK"
     gene            1104..1622
                     /gene="mddA"
                     /locus_tag="JN701_004000"
     CDS             1104..1622
                     /gene="mddA"
                     /locus_tag="JN701_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY7366258.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1619..2068
                     /locus_tag="JN701_004001"
     CDS             1619..2068
                     /locus_tag="JN701_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7366259.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2069..2302)
                     /locus_tag="JN701_004002"
     CDS             complement(2069..2302)
                     /locus_tag="JN701_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY7366260.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2388..2561)
                     /locus_tag="JN701_004003"
     CDS             complement(2388..2561)
                     /locus_tag="JN701_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY7366261.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2756..2851
                     /gene="yncL"
                     /locus_tag="JN701_004004"
     CDS             2756..2851
                     /gene="yncL"
                     /locus_tag="JN701_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY7366262.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          752 a          792 c          723 g          830 t
ORIGIN      
        1 attttatcct ctttcaccca ttgccccatc tccttctgaa actcatggat gcggtgacca
       61 taatcctgag cgataataaa cccttgcaag cgaatacgtt ttttcagcac cgttgccatc
      121 aacaggggta aacgatccgg accgggtggt agctctgtag cgttatagct gctcactaag
      181 ccacaaacgg gaatgcgcgc agatgtatta agtaatggta gcactgcatc aaatacctta
      241 ccgcccacgt tttcataata gatatcaata ccttttgggc acgctttcac cagttgttcg
      301 gcaaaatcac ccgcgtggtg atcaagacaa acatcgaagc ccagcacctc gatagcatgg
      361 cggcattttt ccgcgccacc ggcccccccc accaccctgc aacctttaag tttgccgatt
      421 tgccccaccg ttgctcccac aggacctgtc gccgcagcta ccaccaacgt ttcgccctct
      481 ttaggctgac cgatatccag cagccccata taagcggtaa agcctggcat ccccagcaca
      541 cccagcgacc acgatggatt ttgcggatga tcgccaagtt tcaccagatc atcaccactg
      601 gatatgtcat agtcctgcca tccactgtag cccagcaccc agtcgccagg ctgataatca
      661 ggatgattcg actccacaac atggctcacc gtgccgccga ccatcacgcc gccaatatca
      721 acaggcggtg aataagatgg ctcatcgctc atacgtccac gcatatacgg gtccagggac
      781 aaataaactg tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca
      841 agacggaaat tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga
      901 ttacgctgct tttgttgccc cataatgctc tcctgtgctt ttggcatgaa tccatcaata
      961 gtagcaggct cacgggattg gttttgtttg cagccgtcac atcccctctg caacacgcaa
     1021 taatttcatc gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca
     1081 tcattatttt atcgaggcca gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg
     1141 ccattgcgga aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa
     1201 cggttgatgc agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg
     1261 tgctggtaag tgaagaagat ggcgtagtga cgggatatgc ctcgtttggc gactggcgta
     1321 gtttcgatgg ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg
     1381 gcaaaggtct ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg gatagcggga
     1441 agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccatt
     1501 cgctgggatt tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc
     1561 tggatctgac atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat
     1621 gaatcagtcg ctcacccttg cttttttgat tgccgccgga attggtctgg tagtccagaa
     1681 cacgctgatg gtgcgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa
     1741 ctcactggtg gggattgtgc tctttgtttc tattttatgg tttaaacaag gcatggcggg
     1801 gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt atacccggct tgctgggatc
     1861 gttttttgtc ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc
     1921 agtactggtc gccagccagc taattggcgg tctgatgctg gatatcttcc gcagtcatgg
     1981 cgtaccacta cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc
     2041 ctggttggta gccagacgct cattttaatc agaggatggt gccgcctttg gttaactgtt
     2101 cgtggcgcgc ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca
     2161 aacgctgctg ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga
     2221 ttaataattc gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga
     2281 tgacttcatc cagatgcgac atactttctc cttaaccact tgtgaaattt atcgattcat
     2341 ccaaatgata taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa
     2401 aacaacagcg ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag
     2461 aaacggatgc gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc
     2521 ataaccgcat aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccaga
     2581 tgaataaaac gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttcaccagt
     2641 ttgagaaaaa atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg
     2701 cggattttct taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa
     2761 tgtttccagt agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct
     2821 tatctctatg agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg
     2881 tcagagaaat caactctctg acagtacgca agatcttctc atactctcca ggtttacaac
     2941 attatcctta atatttactt ctttttgctg gcatctacat tgaaatgagt agaaaacatc
     3001 cccctactgt cgtctataca aacagcttac tgattcaaac ggaatcttgt tttcacattt
     3061 ttctatcatg aatactttat acgcgcggga gtctata
//
LOCUS       DADLTD010000303         3084 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLTD010000303 DADLTD010000000
VERSION     DADLTD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.307"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..190)
                     /locus_tag="JN701_004005"
     CDS             complement(<1..190)
                     /locus_tag="JN701_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAY7366263.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQ"
     gene            383..885
                     /locus_tag="JN701_004006"
                     /pseudo
     CDS             383..885
                     /locus_tag="JN701_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(931..1347)
                     /gene="rraB"
                     /locus_tag="JN701_004007"
     CDS             complement(931..1347)
                     /gene="rraB"
                     /locus_tag="JN701_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY7366264.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1509..2513
                     /gene="argF"
                     /locus_tag="JN701_004008"
     CDS             1509..2513
                     /gene="argF"
                     /locus_tag="JN701_004008"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY7366265.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2585..>3084)
                     /locus_tag="JN701_004009"
     CDS             complement(2585..>3084)
                     /locus_tag="JN701_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7366266.1"
                     /translation="RKLQELIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEVP
                     VSILVNDIFLKDFFMKQPDFAKWYFYQLLKKYEGEQLYLNELGYVYGNEEKTNEIVNN
                     NPGYVVEIFEEKMGNELKIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIKK
                     LGWNN"
BASE COUNT          863 a          701 c          659 g          861 t
ORIGIN      
        1 gttgtccgtt aacttccata ttagcataga gataacgctt acatttcatt aatgcccatg
       61 gtaaatagat acctagcgtt ataatcgtta acaacacatt caccacacaa ataagaaaat
      121 atctctcacc tgtaccatga aaaacaaacg aatgggacgg aacatccatt tcattaataa
      181 cttgagccat aaaaattccc tgaattaaaa aatccattga tataatttat atatacagac
      241 agtaaaatgt tctgccacgt tacaatactc ttatcagtaa taggctggca aaaactttat
      301 tgctttataa gatgcttgcg cacgtaagaa gagagtggta ttaaacaaat gtataattac
      361 tttattttta ttgggttaaa ctatgaataa cattgcgccg caatcacctg taatgcgtcg
      421 cctgacgcta caggataatc ctgctattgc ccgcgtcatt cgtcaggtat ccgccgaata
      481 cggtcttacc gctgataaag gctacaccgt cgccgatccg aatcttgacg aactgtatca
      541 agtgtatagt cagccgggtc atgcatattg ggtggttgag tacgagggtg aagtggtcgg
      601 cggcggtggg attgcgccat tagcaggtag tgaatcggac atttgcgaac tgcaaaagat
      661 gtattttctt ccggctattc gcggtaaagg gctggcgaaa aaactggcct taatggcgat
      721 ggagcaggca cgagagatgg gcttcaaacg ctgctatctg gaaacgaccg ctttttaaag
      781 gaagccattg cgctttatga gcatttgggc tttgagcata tcgactatgc acttggctgc
      841 acgggccatg ttgattgtga agtgcggatg ttgcgcgaac tctaaaaaaa tgcccgctag
      901 tgtgtgctat cgggcatttt aatcaattaa ttagtggcga accccgtcat cgtcttcatc
      961 gacaaaatct tcatcgtcgc cgtcttcgcc attaggatct tcaaaataag tgccccaacc
     1021 gtcgtactca acgtcaaatt tctccgccaa tgtcatcagt tgttcaacct gggcatcgat
     1081 cagatcggca ttcaacgcgc actcgctgag gatgtcgcag caaatcacga tatcaccatc
     1141 ttcaacttcc agctcttctg gatcggtcac ttcgtaaccg agtttaaacg cttcaactgc
     1201 tgctttttcc agggtttcca gatcgtctgc ggaaagatgg tgttcgatgg tgtacagcgc
     1261 gtccggatcg ctgccatctt ccagtaattc ttcaataatc aaacgagttt cttcacgctg
     1321 ttcttccagt tgttccgggt ttgccatggc tcattcctct taatgtcctg ccgatactct
     1381 tattgtcaca taccactgac attgcctcca cctttgtgac aaagatttat gctttagact
     1441 tgcaaatgaa taatcatcca tataaattga attttaattc attgaggcgt tagccacagg
     1501 agggatctat gtccgggttt tatcataagc atttcctgaa attactcgat ttcacgccag
     1561 ctgaactcaa cagcctgctg cagttagccg cgaagctgaa agccgataag aaaagcggca
     1621 aagaagaagc caaactcact ggtaaaaaca tcgcgctcat cttcgaaaaa gactcgaccc
     1681 gtacccgatg ctctttcgaa gttgccgcat atgaccaggg tgctcgcgtt acttatctcg
     1741 gcccaagcgg cagtcagatt ggtcataaag agtcgattaa agacactgcc cgcgtgctgg
     1801 gtcgcatgta tgacggtatt cagtatcgcg gctatggtca ggagattgcc gaaacactgg
     1861 cggaatacgc tggcgtgccg gtatggaacg gcctgaccaa tgagtttcac cccacgcagt
     1921 tgctggcgga tctcctcacc atgcaggagc atttacccgg taaagcattc aacgaaatga
     1981 cgctggtcta tgcaggtgac gcacgtaaca acatgggcaa ttcgatgctc gaagctgcgg
     2041 cgcttaccgg tctggatttg cgtctggtcg cgccacaggc atgctggccg gaagctgcgc
     2101 tggttacgga atgccgcgcc ctggcacagc aaaatggcgg gaatattacg ctgacggaag
     2161 atgtagccaa aggagttgaa ggtgctgact ttatctatac cgatgtatgg gtgtcgatgg
     2221 gggaagcaaa agagaaatgg gcagaacgga ttgcattact gcgtgattat caggtgaaca
     2281 gcaagatgat gcagttgacc ggtaacccgg aggtcaaatt cctccactgc ctgcccgcgt
     2341 ttcatgacga ccagacgacg cttggtaaga aaatggcgga agagtttggc ctacatggcg
     2401 gaatggaagt gactgatgag gtcttcgaat ctgccgccag cattgttttt gatcaggcgg
     2461 aaaaccgcat gcataccatc aaagcggtga tggtcgcgac gctcagtaaa taattgatat
     2521 cgcaataaag atgccgggaa atacaaaaac atcccggcat cacattattt tatgcagtaa
     2581 tagttcaatt attccacccc aattttttta ttgctatacg taaatcttcc ggaggattta
     2641 atttttctaa ctgttcttta ttaatatatt cacatatatt tattttccca tcccggagga
     2701 tttccatcat tcttgttcta atttttaatt cattacccat cttttcttca aaaatctcaa
     2761 cgacataacc cggattgtta tttacaattt catttgtttt ttcttcatta ccataaacat
     2821 atcctaactc attaagatac agctgttcgc cctcatactt cttaagtaat tgatagaagt
     2881 accacttcgc aaaatcaggt tgcttcatga aaaaatcctt caaaaaaata tcatttacta
     2941 aaatgctgac aggcacttca ttaaatagca acttaacatc atcaaataac cttagtatta
     3001 agcgtgattt ttcttgattg cttaagttaa aatcattgac atatgcaacc aacttatccc
     3061 aaatcaattc ctgtaattta cggc
//
LOCUS       DADLTD010000304         3064 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLTD010000304 DADLTD010000000
VERSION     DADLTD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.308"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(48..232)
                     /locus_tag="JN701_004010"
                     /pseudo
     CDS             complement(48..232)
                     /locus_tag="JN701_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(394..468)
                     /locus_tag="JN701_004011"
     tRNA            complement(394..468)
                     /locus_tag="JN701_004011"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(433..435),aa:Arg,seq:cct)
     gene            complement(544..1476)
                     /locus_tag="JN701_004012"
     CDS             complement(544..1476)
                     /locus_tag="JN701_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY7366267.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1770..2525
                     /gene="mlaA"
                     /locus_tag="JN701_004013"
     CDS             1770..2525
                     /gene="mlaA"
                     /locus_tag="JN701_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY7366268.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2707..3042)
                     /locus_tag="JN701_004014"
     CDS             complement(2707..3042)
                     /locus_tag="JN701_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366269.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLS
                     APGVNFDDV"
BASE COUNT          766 a          706 c          712 g          880 t
ORIGIN      
        1 atcttttacg gggtgaaggt agatttcgta tgaagggtat ctgttgtctc ctgcaaagtc
       61 ataagcggat attttcctac ggtcagtatt tttttcttac cgtcaatctt gtagcgaagt
      121 tgccatacct tttcccggat acaggaacat aaaggtacag gccattacca tcgagaaggc
      181 ggtatggttt ttctttcggc tttgctgctt caatctgctt aacggtgagc atgggtaaaa
      241 atccggtggg taaaattatt ttatccactt tttacccgtc atggagtgcg gctgtcaacg
      301 atctgacgcg aaccatgacg aaccgtgaat atacggaagg cttgatattc aggggatttt
      361 gcggactggt acggatggga gcgaactgat aaatggtgtc ccctgcagga atcgaacctg
      421 caattagccc ttaggagggg ctcgttatat ccatttaact aagaggacaa tgcggcatga
      481 gtatacccgc taatggactg cggagtaagt acactgccgc tcgattgctt aaaccctcgc
      541 catttatgct gggtttttat catttttctt aatgttttcc gcacgttctg ctttttggcg
      601 tgcttctgct ttgcgcttgt tgctcatgtc gttacggatc tgtgcatgac tcattaacgc
      661 gaagataaag gtgccgccgc agatattccc cgctaaagta ggtagtgcga agggccaaat
      721 gaaatcgctc cagtgcagcg tgccgttaaa caccagatag aggatttcta cagaaccgac
      781 aacgatgtgg gtggtgtcac ccagtgcaat aagccaggtc atcaatataa tcaccacaat
      841 ctttgccgca cccgctgcag gaaacatcca aaccatagtg gcgatcagcc agccggaaat
      901 gatcgcgttg gcaaacatct cgctgggggt gttcttcatc acatccatgc cgattttgac
      961 aaatgcatcg tgagtttctt cattgaagat aggcatatat tcaaatgccc atgcagcaat
     1021 acctgtcccg agaatattac ccagcagcac gacgccccat aatcgcataa gtaagccgac
     1081 gttgctcatt gtcggttttt gcatgacggg tagtaccgca gtcacggtgt tttcggtaaa
     1141 taattgctgg cgggccataa tgacgataat aaaaccaaag gtataaccga gattctccaa
     1201 caagaagctg cccggcacgc cttccagttc gacatgaaat atcccttttg ccagtaacga
     1261 agcgcccatc gacagacccg ccgcaatggc tgaccacagt agtgccattg cgtcgcgttc
     1321 cagctctttt tcaccatcct ggcggatatg ttcatgaatt gccatcgccc gggaggggag
     1381 tcgatcttca tctatttcta tttttttgcc gcgctctttt tcttcgctct caacttcaat
     1441 ttcgtcgctg tgttgatcaa ttttgtcgtt gtccatggtc tcttctcttg aattagcacg
     1501 tatagctaaa gcgtagcggc ttttttgctc gcaactggcg ggagttactc tgaaaatgta
     1561 gaaaaggccg cgtttgcctt tttctgtttc tatagaatca agtagcctac agggcggcga
     1621 ttaccaggct atgatcaaat cagcaaatca gggcgtctgg acatcagttg acgtgctgtt
     1681 acaatcgccc acacctaaac aggcggatac ggtatcgttc cgtcatggat ggcaaactgc
     1741 ataagccata aaaaaacagg gagacattta tgaagcttcg cctgtcggcg cttgctctgg
     1801 gaactacgct tctggtgggg tgtgcgagtt ccggtacaga tcagcaaggg cgttctgacc
     1861 cgttagaagg gttcaaccgc accatgtaca acttcaactt caatgtatta gacccgtata
     1921 ttgttcgacc ggtcgctgtc gcctggcgtg attatgttcc gcaaccggcg cgtaacggtt
     1981 tgagcaactt taccggcaac cttgaagaac ctgcggtgat ggttaactac ttcttgcagg
     2041 gcgaccctta tcaggggatg gtccacttta cccgcttttt cctgaacacc attttgggga
     2101 tgggcggctt tattgatgtt gcagggatgg cgaacccgaa actgcaacgg actgaacctc
     2161 accgcttcgg tagtacgctt ggtcattatg gcgtgggtta tgggccttac gttcagttac
     2221 cgttctacgg tagcttcacg ctgcgtgatg acggtggtga tatggcggat ggtctttacc
     2281 cggttctttc ctggctgacc tggccgatgt ctgtgggtaa atggacgctt gaagggatcg
     2341 aaacccgcgc tcagctgctg gattccgatg gtctgctgcg tcagtcgtcc gatccttata
     2401 ttatggtgcg cgaagcgtac ttccagcgtc atgatttcat cgctaatggc ggcgaactca
     2461 aaccgcagga aaatccgaac gcacaagcga ttcaggatga tttaaaagat attgattctg
     2521 aataagaaac aaataaaaaa ggtgagtcgc aaaactcacc ttttttgttg ttatccctca
     2581 attaccacaa gtaaaaggag ataaaccgac aatgacccgg acgtaatgat gtaaaaatac
     2641 tgatgttcgt cactgactat ttccgatatt caggcgttga aaaatcaaaa aactggaaaa
     2701 ataattttaa acatcatcaa aattaacacc tggcgcagat agttgattaa tttcattttg
     2761 atattccgtc tggcgatttt gaagcctttt ttcaacttta tcttcgagat ccttaaagtc
     2821 tttgtattta ttttcaggat ggtcaacgtc aactttgtta gcgaataaag tagaaattat
     2881 ttcttttaca ttatcgcgtc taaattttgc atcgtccact tgaaaattat atcttattgc
     2941 tcgacctaaa cgtttaaaaa cttcgttagg ttctctcagt ctttccccgt tgagctcgtt
     3001 tacaacctga tgatcggaac ctgttgcaaa tgattctgcc atgatagctt tactgctttc
     3061 aaac
//
LOCUS       DADLTD010000305         2958 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLTD010000305 DADLTD010000000
VERSION     DADLTD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.309"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..883)
                     /gene="mltB"
                     /locus_tag="JN701_004015"
     CDS             complement(<1..883)
                     /gene="mltB"
                     /locus_tag="JN701_004015"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY7366270.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQ"
     gene            1139..1702
                     /gene="srlA"
                     /locus_tag="JN701_004016"
                     /gene_synonym="gutA"
     CDS             1139..1702
                     /gene="srlA"
                     /locus_tag="JN701_004016"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY7366271.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            1699..2658
                     /gene="srlE"
                     /locus_tag="JN701_004017"
                     /gene_synonym="gutE"
     CDS             1699..2658
                     /gene="srlE"
                     /locus_tag="JN701_004017"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY7366272.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGTVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            2669..>2958
                     /gene="srlB"
                     /locus_tag="JN701_004018"
                     /gene_synonym="gutB"
     CDS             2669..>2958
                     /gene="srlB"
                     /locus_tag="JN701_004018"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY7366273.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFP"
BASE COUNT          661 a          804 c          752 g          741 t
ORIGIN      
        1 gctgcgaaat gctgtacctg gttttgaagc catttggcaa gcctggagcc tgaccgtttg
       61 ccattaccgc gacctgatcg cctttcaccc agccgtgcgc tttgaaatag ttcgccacgc
      121 taccgatcgc atcaaccgga tcccacaggt tgatatgccc gtcgccgctg aaatctaccg
      181 catattgttt gtaagacgac ggcataaact gtccgtagcc catcgccccg gcaaaggaac
      241 ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat cagcaggaag gtttccagct
      301 cgccagagaa atactccgcg cggcgtgggt agttaaatga cagcgttgcc agcgcatcga
      361 ggatgcgagt tttccccatc acgcgccccc agcgggtttc aacgccgata atcccgacga
      421 taatttccgg cggtacgcca tacacctgcc acgcgcgatt caacgcatct tcatactgat
      481 tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat aaattttttg cgataacgca
      541 gccatgcgcc gttcggacct gatggcggtt tcaccgatgt ggttggtgcc tggttatcca
      601 tcagccgcag taccgaatcc agacgcttcg cctgggagag aatttcctgc aactgctgac
      661 gatcgaaacc gtgtttgttc accattttgt cgatgaactg ctgggcattc gggttattag
      721 cgaaatcgcc gcccatctgc atcacattgt gctgcggctc aagcaggaag ccgccagacg
      781 gcgttccggt ggtcgtttca gtctcagtag gttttggctt gctgctacag gcggcaagca
      841 acacaaaaag gggaagcaat gttacataac gacgcttgaa catgaggggt ccatttaaca
      901 gattcaacca ggggcaagta tggtaaagca tcacgccccg cacaaggaag cggtagtcac
      961 tgcccgatac ggactttaca taactcaact cattcccctc gctatccttt tactcaaact
     1021 tccaaattaa aatatttatc tttcattttt agattaaaat aacactttta aatctttcaa
     1081 tctgattaga ttaggttgcc gtttggtaat aaaacaataa atcctgaagg agagaacaat
     1141 gatagaaacc attactcatg gtgcagagtg gtttatcggg ctgttccaaa agggcggaga
     1201 ggtgtttacc gggatggtga ccggcattct tccgctgttg attagcctgc tggttatcat
     1261 gaacgcactg attaatttta tcggtcagca tcgtattgaa cgttttgctc aacgttgcgc
     1321 cggtaaccct gtttcccgtt acctgctgct accgtgcatt ggcacgtttg tcttttgcaa
     1381 tccgatgacc ttaagccagg gtcgctttat gccggaaaag tacaaaccca gctactacgc
     1441 ggcggcctct tatagctgcc actcaatgaa tggcctcttt ccccatatca accctggcga
     1501 actgtttgtt tatcttggca ttgccagcgg tctgacaacg ctgaacctgc cacttggccc
     1561 actggcggtg agctatctgc tggttggtct ggtcaccaac ttctttcgcg gctgggtgac
     1621 cgatctgacc accgccattt ttgagaaaaa gatgggcatt caacttgaac aaaaagttca
     1681 cctggcagga gcaacatcat gacgcgtatt cggatcgaaa aaggaacggg tggctggggc
     1741 ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg tctatatcac cgccggtacc
     1801 cggcctgcga ttgttgacaa actggcacaa cttactggct ggcaggctat tgacggattt
     1861 aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg taatcgactg tggcggcaca
     1921 ttacgctgcg gcatctatcc gaaacgacgt attcccacca ttaatatcca ctcgacgggc
     1981 aagtccggtc cgctggcgca gtacattgtg gaagatattt atgtctctgg cgtaaaagaa
     2041 gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct cttccgtggg ccgtgactat
     2101 gacaccagta agaaaatcac cgaacaaagc gatggtttac tggcgaaggt gggaatgggc
     2161 atggggtccg ccgttgcggt gctgtttcaa tctggtcgtg acaccatcga cactgtatta
     2221 aaaaccattc tgccgtttat ggcattcgtc tcggcgctca ttggcatcat tatggcttct
     2281 ggccttggtg actggattgc ccacggtctt gctccgctgg cgagccatcc actgggtctg
     2341 gtcatgctgg cgctcatctg ctccttccca ctgctttcac ctttcctcgg cccaggcaca
     2401 gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga ttggtctcgg caatattccg
     2461 ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc aggcggcctg cgacttcatc
     2521 ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg ttcgcgtcgg tgtcccttct
     2581 gtactggtga gccgcttttt aaccggcgca ccgactgtac tgatcgcctg gtttgtctcc
     2641 ggttttatct atcaatagag gctgaaacat gaccgttatt tataagacca ccatcacccg
     2701 tatcggcgcg agtgccactg acgccctcag cgaccagatg ctcatcacct ttcgtgaagg
     2761 cgcgccagcg gacctcgaag agtattgctt cattcattgc cacggcgagt tgaaaggtgc
     2821 actccatccc ggtttgcaat ttacgctcgg gcagcatcgc tatccggtga ccgctgttgg
     2881 cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc accctgcgct tcgatggttt
     2941 aaacgaagcg gaatttcc
//
LOCUS       DADLTD010000306         2942 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLTD010000306 DADLTD010000000
VERSION     DADLTD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.310"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            577..1293
                     /locus_tag="JN701_004019"
     CDS             577..1293
                     /locus_tag="JN701_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366274.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1845..2249
                     /locus_tag="JN701_004020"
     CDS             1845..2249
                     /locus_tag="JN701_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366275.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2312..2611
                     /locus_tag="JN701_004021"
     CDS             2312..2611
                     /locus_tag="JN701_004021"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7366276.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAVQAEELAILQKAATYFAKRLK"
     gene            2608..>2942
                     /locus_tag="JN701_004022"
     CDS             2608..>2942
                     /locus_tag="JN701_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7366277.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRALRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASRK
                     FSPVSYRAHG"
BASE COUNT          824 a          605 c          668 g          845 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttggtcact tgtctggttt tgatagtagc attctgcaga gtattttgat atgataaata
      481 tgcattaaca atgatattat tgttaatttc ctagcatgaa aattaaaaga tcaacttttt
      541 atctcgaata ttttttacat tatcagttgg tttcttatga atgacaattc attgcttaga
      601 aattcttcat tattcatcgc ctatatggga tgtgtagggt gggtcagtgc ttattcttat
      661 ggctggggaa cctcttttta ctatggattt ccatggtggg ttgttggtgc aggtttggat
      721 gatgttgcca gaagtttact ctatgctatc atagtaatgg gtatactttt taccggttgg
      781 gggattggta ttttattttt tcttcttata aagaaaagaa gtaaaataca agacttgagt
      841 ttttttagac tattttttgc tattacccta ctgtttttcc ctgtaatatt cgaactttta
      901 attctcaaac aatatttcat acttccatta tcactttcat gcattattag ttcattggtt
      961 atttcaataa taattcgcat atatggacgg atcttttctg tatcatgttt ttcagatatc
     1021 ccttttgtta gagaacaccg cattaagtta attatggctg gttttttggt ttatttttgg
     1081 cttttttcct ttcttgttgg ctggtataaa cctcaattaa aaaaagaata tcagatgctt
     1141 tgttataata atagttggta ttatgttctt gctcgttatg attcacggct cgttttgtct
     1201 agttcattta aagatgacag taacagattc ttaatattta atacagaaca gtcgggattt
     1261 tatgaaatca atgatgttta tgtcagaaag taataatcct ctcccacaaa gtgaattaaa
     1321 cgaatatata atgcgttcga agtatatgta ttgagctgtc atactgagaa acaaacctta
     1381 gggcagcaca attaaagcca gaaacaacta tgcaagaaag ttactaaata agtgctcgga
     1441 acagtaaaac actcaggggg gactcagtcc agattatgta atcagataaa ttgccaaaca
     1501 tcacaaatca ccagcaggac tgagtgatgc cgagcattgc accaataccc cgtgatgaac
     1561 gatgcctgat gcagaaagct ccctataaaa caaagaacaa aaactacgac cggcgattca
     1621 ccgtcatact gatgctgcat cgtggtgaca ctgtctgtca ctttcatgtt gactgaacaa
     1681 ctctgctgcg aagaaggtgt accgccggcg ggatttgatt gtccggcgtc tagcttccac
     1741 ttccctgatg gaactgaaag ttgttatggc cggtcagctt gccctgtccg caggacatga
     1801 ggtgctgtga cggacagcac cagtaacgga aagtgacaat aaaaatgaat actgcgctga
     1861 tagttgcact aatgtgtatg tggtatgcgg tacccgctgc tgcaaaagag actttactgg
     1921 ctatgccacg taattcaaca gaacattgct atgcggagat taatgtgcac ggaccatatg
     1981 gggtgtattt ccgagtggtc ccgcatcccc ccgggggaaa gagctgggtc gaatgtaaca
     2041 gtgactacta ttactctgat aaaccaccgg gagtacagat actcggaacc cgtgcagggt
     2101 gtcgggtata cggaatctgc gggaccacct cgacactgca tgtggctgga agaggggttg
     2161 tgtgtattaa aaatatatgc agtccacgtg ggatgataat tcacaggata cggaaacgtc
     2221 ctgtagtggc tgtaagcgat gagatgtgat cttatccagc aatagtggac acgcggctaa
     2281 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagcaaaaa
     2341 aacacgtaaa cagcattcgc ctgaattttg cagtgaagcc ctgaagcttg ctgaacgcat
     2401 cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct atgcgtggcg
     2461 cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg ccgctgaaaa
     2521 tgcccgcctg aagcggcaac tggcggtgca ggcagaggaa ctggctatcc tccaaaaggc
     2581 cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2641 gagttcagca tcaaggcaat gtgccgcgcg ctccgggtgg cccgcagcgg ctggtatacg
     2701 tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc
     2761 gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc acgcctgacg
     2821 gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc
     2881 cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat
     2941 gg
//
LOCUS       DADLTD010000307         2918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.311, whole genome shotgun sequence.
ACCESSION   DADLTD010000307 DADLTD010000000
VERSION     DADLTD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.311"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1322
                     /locus_tag="JN701_004023"
     CDS             <1..1322
                     /locus_tag="JN701_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7366278.1"
                     /translation="LVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTM
                     GPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLF
                     IFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFI
                     PHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSL
                     AMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSID
                     APLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLYK
                     WLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFA
                     CLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1323..2231)
                     /locus_tag="JN701_004024"
     CDS             complement(1323..2231)
                     /locus_tag="JN701_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7366279.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2331..>2918
                     /locus_tag="JN701_004025"
     CDS             2331..>2918
                     /locus_tag="JN701_004025"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HAY7366280.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          738 a          622 c          728 g          830 t
ORIGIN      
        1 ggctggtggt tgttttttca tgggtgttta tctttgcgct ctatttcaca ccttatgcgc
       61 taattgttgg tcagttaggc tcgaccttca aagatgggaa gggcggggtc agtacctgga
      121 ttaaacacac gatgggaccc ggactggctt atctcgccgc gtggacctac tgggtggtgc
      181 atattcccta tctggcacaa aaaccccagg caattctgat tgcgctcggt tgggcgatga
      241 aaggcgacgg ttcgctaatc aaagaatatt cagtcgtagc gttacagggg ttaacgctgg
      301 tgctgtttat cttctttatg tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg
      361 gttctgtggc agggattgcg atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa
      421 ccgcgcctgc gattactgaa gtgcatattg cgaccacaaa cattacctgg gaaacgttca
      481 ttcctcatat cgactttacc tacattacca ctatttcaat gctggttttc gcggttggcg
      541 gagcagagaa gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa
      601 aagggatgtt atgcctggcg gtgatggttg cggtttgtgc cattctgggc tcactggcga
      661 tggggatgat gtttgactcg cgtaatatcc cggatgactt aatgactaac ggtcaatatt
      721 acgcctttca gaagctgggc gagtattaca acatgggtaa tactttaatg gtgatttacg
      781 ccattgcgaa taccctggga caagtagctg cgctggtatt ctcgattgat gccccgctta
      841 aagtgctatt aggtgatgct gacagcaaat atattccagc cagtttatgt cgtaccaacg
      901 cttctggtac gcccgttaat ggctattttc tgaccctggt gcaggtggca attctgatta
      961 tgctgccgac tctcggcatc ggtgatatga acaatctcta taaatggctg ttgaatctta
     1021 actcggtagt gatgccgctg cgttatttat gggtatttgt tgcatttatt gcagtcgttc
     1081 gcctggcgca gaaatataaa ccagaatatg tctttattcg taataagcct ctggcaatga
     1141 ccgtcgggat ttggtgtttt gcctttaccg cctttgcctg tttgacgggg atcttcccga
     1201 aaatggaagc cttcactgca gagtggacct tccagttggc gctgaatgtt gcaacgccgt
     1261 ttgtgctggt aggattagga ctgattttcc cgctgctggc gcgtaaagcg aatagtaaat
     1321 aattattgtg gtcggtgacg ctgactctgg ctgatctgac ccgtttttac ataatcgaga
     1381 aatgcgcgca agccgcagga catatactgg cggtttggat aatagatctg gaagcctgga
     1441 cgctctgtgc tccagtctcc cagtacacat tctaaacgac cagtatcaag gtactccttg
     1501 atctgttcat agagcagata cccgatacca gcacccatca aaacggcctc cagttcagca
     1561 tcaacatcat ccagaatgat atttcctgcg acagcaattt ccagctcctt tgcaaattgc
     1621 cagtggaaag gtttgccgct gggataacga aaaacaacac attgatgatt aagcagatca
     1681 tgaggatggc ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat
     1741 ttcactggcg ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga
     1801 acgccagcgt caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg
     1861 actttaatat caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt
     1921 gcagctaccc gggcgtcatt gattttgagc gttcctgtcg gtgtcagacg aaaatcattc
     1981 atctcatcta acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta
     2041 gaacccgctt ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga
     2101 cgttgttcca gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg
     2161 cccgctgcac gaaagctttg attacgggcc acggcaaaga aagtggcaaa gtcagacata
     2221 ttcatccgca ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacaggat
     2281 tatgccgcct gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg
     2341 aacgtattta tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg
     2401 atgcgatcca tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc
     2461 ggcgtaattt caacccagtg atgacgccgg aagatgaacc agactggaag aatatggata
     2521 aacgttattc tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag
     2581 tggtggtttt tcctctctgg tggtacagct tcccggcaat gctaaaagga tatattgaca
     2641 gagtatggaa taatgggctg gcttatggag atgggcacaa attaccattc aataaagttc
     2701 gttgggtggc gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa
     2761 atataagcga ttatttgaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca
     2821 ctttcttgtg taatacagtg gtattcgatg gggaagaact tcatgcgagc tattatcagt
     2881 cgttattatc tcaggtacgg gatatggtag atgcacta
//
LOCUS       DADLTD010000308         2839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.312, whole genome shotgun sequence.
ACCESSION   DADLTD010000308 DADLTD010000000
VERSION     DADLTD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.312"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..134
                     /locus_tag="JN701_004026"
                     /pseudo
     CDS             1..134
                     /locus_tag="JN701_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            384..1250
                     /gene="ospB"
                     /locus_tag="JN701_004027"
     CDS             384..1250
                     /gene="ospB"
                     /locus_tag="JN701_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY7366281.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1580..2320
                     /locus_tag="JN701_004028"
     CDS             1580..2320
                     /locus_tag="JN701_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7366282.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2680..>2839)
                     /locus_tag="JN701_004029"
     CDS             complement(2680..>2839)
                     /locus_tag="JN701_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY7366283.1"
                     /translation="LEYFSNNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKK
                     WSGIRQTI"
BASE COUNT          928 a          518 c          526 g          867 t
ORIGIN      
        1 cggtaagaga agttcatcat acttggcgca gttacgcgat gtttctgcta gaggtgatgc
       61 cgtaaagaac ggcagaacat taccggaaca agatagcggt ctacctgcac tggtatcaga
      121 cccggggctt ccccggatga tctccacggt actttaggta ctgtgtctat attatgtagg
      181 taggatcaat taactttgta gttgaaattc tttcattgaa ataatgaata aaaaagacaa
      241 accgcattaa acaatgccag aatataattg catttttgtt ccgttttttt aaatcatagt
      301 atacaaagaa gcttgcatac tatatgaaaa tataacaccc tttactatga tgtttataaa
      361 acaatatatg gagtcatgta ggtatgaatt tagatggtgt tagaccatac tgtagaacag
      421 tcaataaaaa gaatgaaagc atatcagata ttgcatttgc acatataata aaaagggtaa
      481 aaaattcatc atgtactcac ccaaaagcag cattggtttt tttaggagag aaaggttttt
      541 gtgatagcaa tgatgttcta tctattatgg gacaacaaat accaagagta tttaagaaca
      601 agatgttata tgattatgtt tttaaaaatg aaaaaagtaa aaatgatttt ctaaaaatgg
      661 ctgaatcatg gctaccacag agtgaaccaa tagtaataaa taatgatgat gacgcattga
      721 atgctgctgc ttatttttct gtaaaaaaag cgaaaataaa aacagtaaac gatactgatt
      781 ttaaagagta taataaggtt tatattcttg ggcacggtag tcctggttct catcaattag
      841 gccttggttc ggaacttatt gatgtacaaa caatcatttc aagaatgaaa gactgtggta
      901 ttctaaatgt gaaagatatc cgttttactt catgcggctc cgctgataaa gtggctccta
      961 aaaattttaa caatgcccct gctgaaagtc tttcttgtat ccttaactct ctgccttttt
     1021 ttaaggaaaa agaatctttg ctagagcaga taaaaaaaca ccttgaaaac gatgagtcat
     1081 tgagtgatgg tctaaaaata tccggctatc atggatatgg agttcactat ggtcaagagc
     1141 tttttcccta ctcacattat cgttcaactt caattcctgc tgatccggag catacagtaa
     1201 aaagaagctc tcagaaaaag acttttatta ttaataaaga actggattag tataaaattt
     1261 ttaacctata gttaattgtt tgcatttaag atgaagttat ttttttcttt agtagaaaag
     1321 tgcttcccta tgagggaaaa tcacaaatca tcataataca gagacaaaac gatacgaaca
     1381 aatagataaa aaacatcatt cttttactac attatttttc gtgaatatga aaaataatgt
     1441 tattacttta atataagact attttttgtt tttccatcac tctgttcaaa tttttccgca
     1501 tgacttgtgt tttttgtaac acagctcgtt ttttacagct gaccaaaatc atcaattaat
     1561 tatgctaagg aaataaatta tgaaaaccaa aaactttctt cttttttgta ttgctacaaa
     1621 tatgattttt atcccctcag caaatgctct gaaggcagaa ggttttctca ctcaacaaac
     1681 ttcaccagac agtttgtcaa tacttccgcc gcctccggca gaggattcag tagtatttca
     1741 ggctgacaaa gctcattatg aattcggccg ctcgctccgg gatgctaatc gtgtacgtct
     1801 cgctagcgaa gatgcatact acgagaattt tggtcttgca ttttcagatg cttatggcat
     1861 ggatatttca agggaaaata ccccaatctt atatcagttg ttaacacaag tactacagga
     1921 tagccatgat tacgccgtgc gtaacgccaa agaatattat aaaagagttc gtccattcgt
     1981 tatttataaa gacgcaacct gtacacctga taaagatgag aacatggcta tcactggctc
     2041 ttatccctct ggtcatgcat cctttggttg ggcagtagca ctgatacttg cggagattaa
     2101 tcctcaacgt aaagcggaaa tacttcgacg tggatatgag tttggagaaa gtcgggtcat
     2161 ctgcggtgcg cattggcaaa gcgatgtaga ggctgggcgt ttaatgggag catcggttgt
     2221 tgcagtactt cataatacac ctgaatttac caaaagcctt agcgaagcca aaaaagagtt
     2281 tgaagaatta aatactccta ccaatgaact gaccccataa agctggacag cctgtatcag
     2341 gctatggagg gcccatagac aaatctaccc tatatgagca taggaggagt ctatgggcac
     2401 accacgtttt acccctgaat ttaagggatt actggaaagg ctgggacata tccctccggc
     2461 agaagcagaa aaagcttatt atgctgccat cggaaacgat gatctggcaa cctgagttca
     2521 cagataaaac attctctagg aaactcgggg cggttccgtt caccacatgc aatgtggtgt
     2581 tgcaggggaa cggtctgcac catcccctat gtcgatcaat ataacagaaa tgacaacttc
     2641 agattcaggg cacaacctaa atatatttta ggtcacctct caaatcgttt gcctgatacc
     2701 gctccatttt ttaataaaaa aatcaatcat ttttgaatct ttgcttttca ttgcattgtc
     2761 aagcatagtg tccccactat ttgctttttg aaattttttg tttacatcaa ccaatccatt
     2821 attgctaaaa tattctagc
//
LOCUS       DADLTD010000309         2787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.313, whole genome shotgun sequence.
ACCESSION   DADLTD010000309 DADLTD010000000
VERSION     DADLTD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.313"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(924..1595)
                     /gene="queE"
                     /locus_tag="JN701_004030"
     CDS             complement(924..1595)
                     /gene="queE"
                     /locus_tag="JN701_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY7366284.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1734..1874
                     /locus_tag="JN701_004031"
     CDS             1734..1874
                     /locus_tag="JN701_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366285.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1888..2760
                     /locus_tag="JN701_004032"
                     /pseudo
     CDS             1888..2760
                     /locus_tag="JN701_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT          857 a          573 c          518 g          839 t
ORIGIN      
        1 tttaactagt tcggtttatc cctgttttta caaggaatat taactacact cgatgtcttt
       61 agaataaaag acaatacgaa tcgcacttta tcaattttca cttaaagtat gaactggata
      121 tctttattca taagaactat tcatcaacag cgtatagagg cggttatgta aaaccactca
      181 ttagcctcaa aacaaagaca aacttccaga tttattactt aaccaatgaa taacaagctt
      241 aaaaaatata ctgttattct aaagaaaaaa ttaacaggca ttaattcaat attaaaaaaa
      301 taatagatta aaatttctta acgatttaag aatcatacaa ataacacttt gattaaattt
      361 aattttttgt atcgataatt gtgaacctct ctggcatgga gaactatttt gaacatgagg
      421 tgttacgtgg atatgttgct tattacaagt actgctaata taaaaacttg agaaagagat
      481 aacgggttat atggtggttt atccccgctg gcgcgggaaa ctcccagctc ttgcccgcct
      541 caaagtgttg taactcggtt tatccccgct ggcgcgggga actctgtaaa cataacctat
      601 tattaattaa tgatttttta agccagtaac aatctaccaa ctttacagta tcacacaaac
      661 aacacatcca ttatgttaaa gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa
      721 taactctata caaattaaaa tctaccaaac tttaccgcaa taattttcac tccagcgaaa
      781 aattaatgcc acagaatttg tagataatgc taatgattag ccctggatgg gtaaaatgga
      841 ggagttttca gaggcgacaa acaatataaa tgagtagaag tctttacagg ttccacccat
      901 ttataacgct tataaatgtt taatcaggca atatttagat atttatgtgt ttgcatcgac
      961 aaacgccaat tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg
     1021 ctaatcggct gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc caatagttca
     1081 tcgagtgctt caatatcgcg tacgcgcccc actggatgct tgatttcgtt ggctcgctcc
     1141 agtgcctgtg acaacacgtc atagccgccg cgcatgttca gctttggcga tacggtaacc
     1201 caggtattcg gtgtgcagcg tacttcatga gttccactgg tttcgatctg gcagctaaaa
     1261 ccgttctttt cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc
     1321 gtaatcacca catgccgcgc ggtatatccc tgccgaccaa tgacagccag caaatcttca
     1381 ctgctcgccg ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag
     1441 acttcccgat cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc
     1501 gggcatccct gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc
     1561 agggtctgga acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt
     1621 gttaattatt gcagatcctg ccgcaacaat catgccttat taacattctg ttacatgcag
     1681 atttaaagaa aaatgcgaaa catatcgtta ataattaaag ggagtaacgt agtatgtcag
     1741 aagaaaataa agaaaatgga tttaatcatg tcaaaacatt caccaaaatt atatttattt
     1801 tttctgtatt agtttttaat gataacgagt ctaaaattac cgatgccgcc gtcaatttat
     1861 ttatccagat ttaatgagaa atttataatg cgctatttca tactaatgtt cactttcgta
     1921 tgttcctttg ttgcagccca accaactatt gtcccacaat tacagtaaca agttacggat
     1981 cttacgagta gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt
     2041 ttcaataata cccaagtgca aattgctgta ttaattgttc ccacaaccaa agacgagaca
     2101 attgaacaat atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat
     2161 gatgggatac tgatcattgt tgcctggtca gatcgtactg tccgcatcca ggtaggttat
     2221 gggctggaag aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata
     2281 cccgcattta aacaacaaaa atttgctcag ggattagagc tagctataaa cgctttgaat
     2341 aaccaactca cttcacaaca ccaatatccg actaatcctt cagaaagtga atcagcgtct
     2401 tccagtgatc attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc
     2461 tggttttttc atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca
     2521 gcaatctatc ttttagattt attcctgttc tccgataaaa ttttttccat tgctgtattt
     2581 tccttctttt ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa
     2641 agagcatcag gtaaaagtta tcattcagac aacagcggtt ccggtggagg ctcaagtggc
     2701 ggcggattta gcggcggtgg cggttcttct ggaggtggcg gcgcatccgg ccgctggtaa
     2761 ctcaaccata aaaaatgcca tcccgga
//
LOCUS       DADLTD010000310         2783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLTD010000310 DADLTD010000000
VERSION     DADLTD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.314"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            55..579
                     /locus_tag="JN701_004033"
     CDS             55..579
                     /locus_tag="JN701_004033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366286.1"
                     /translation="MGNLPSIAKTFPMEIHTTILQQLNPLEHISNAISRICYYKEQCK
                     ALELESLKIKEQASFMHLKIDTNFKKAMYELETRRKSNELKLIAAANDFKQISITKSD
                     LIKEISKLVSSICSNDVSLESKKAMNDTLIIMKGMLSDQRSESYEKFKSLLSSIDSSY
                     NDALLIEDHQDNYV"
     gene            572..1153
                     /locus_tag="JN701_004034"
     CDS             572..1153
                     /locus_tag="JN701_004034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366287.1"
                     /translation="MFNIILGVAAVGVGLYILNELDDRAGEEKKRWENQRESVCKSIE
                     EHKRYIEQHVEYVQSMYDFHQLVDLHYSCMKVANEAYKLLNDAKKSMSAIYDAINKLK
                     AHRSKLFNDKKESDDYEKKKEIQAEINMIKEIIDGLYSELDLIKKDKEHFYQELKAFN
                     LKTSSIKYRIRDNTGYKGFDWYNRLQERTLLRR"
     gene            2133..2609
                     /locus_tag="JN701_004035"
     CDS             2133..2609
                     /locus_tag="JN701_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7366288.1"
                     /translation="MFIDSEKRLKQLSDEAKKSTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          909 a          547 c          621 g          706 t
ORIGIN      
        1 aaaaaaaata acgtgccaat gatacaaaat aatggaaatc tccaaccatt tagcatgggt
       61 aatttaccat ccattgctaa aacattccca atggaaatcc atacaactat acttcaacag
      121 cttaacccat tggagcatat atccaatgct ataagtagga tttgttacta taaagaacag
      181 tgtaaagcac ttgagcttga gtcactaaaa ataaaagaac aagccagttt tatgcacctt
      241 aaaatagata ctaattttaa aaaggcaatg tatgagctag aaacaagaag aaagagtaat
      301 gagttgaagt tgatagctgc tgcaaatgat tttaagcaaa tatcaataac aaaatcagac
      361 ttaattaaag agatatcaaa gcttgtttca agcatatgta gcaatgacgt ttcgttggaa
      421 agtaaaaagg cgatgaacga tactcttatc attatgaaag gcatgctcag tgatcaacgg
      481 tctgagtcat atgaaaaatt caaatctttg ttaagtagta ttgattctag ttacaatgat
      541 gcgttgttga tagaggatca tcaggataat tatgtttaat atcatactcg gagttgctgc
      601 tgtaggtgtt ggtttatata tcttgaacga acttgatgat cgcgccggag aagaaaaaaa
      661 gcgatgggaa aatcaacgag aatcagtatg taaaagcatt gaagaacata agagatatat
      721 agaacaacat gtcgaatatg ttcaatcaat gtatgatttt catcagttag ttgaccttca
      781 ttattcatgt atgaaagtgg caaatgaagc atataaactt cttaatgatg ctaaaaaatc
      841 aatgagcgct atttatgatg ctatcaataa actcaaagct caccgctcta aattatttaa
      901 tgataaaaaa gaaagcgatg attatgaaaa gaaaaaagaa atacaagcag aaataaacat
      961 gatcaaagaa atcatagatg gcctatactc tgaattagat ttaattaaaa aggataaaga
     1021 acatttttat caggaattaa aagcttttaa cttaaaaact agcagcatta aatacagaat
     1081 tagagacaat accggatata aaggctttga ttggtataat cggttacaag aacgaacttt
     1141 acttcgacgt tagttttgca ctgaaactat atcagataac aatcctccac ggttccggca
     1201 gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc agacccccat cgctgcaaca
     1261 tcgacggtct gcaccacctg ccaaccgagt aactgcctgt tccgtgcgaa tgggctttgc
     1321 agggggctgg cgcggttcct ggcgacctga aagcccctgg ttaggggtat cggcgcgcct
     1381 gacctcctga gagcctctgt aacgcatttt cgcgcctccc atgccctggc gggcatttaa
     1441 aacccgtttc gtgcgtctga gagcgtttga agggctatct gacgactcat ccccgaaaga
     1501 acaacgacgg aatcaccgac aacatgcgag aggtggcacc gctcgccgca gccccagcga
     1561 ccgaacgtta gtgagcgagt gggcgaggaa gcggaagaga accggtagtt agattgagca
     1621 cgtacgcact gatgggtgtc ggggcgcagc cctgaccaga tggcaaacgg aacatcgtcg
     1681 tgggtgacgg tattacattt gcacatcctg tcccgatttc tgaggcattt tagccttcat
     1741 acccctttta gtggtgaacg gacggcaaaa acatgatgga actgttgaac gtggagagcg
     1801 cgaaatcgga caaaaattac ggttgacgag gcttgcagag gagtaagcca gtatacactc
     1861 cgctatcgct acgtgactgg gtcagggctg cgccccgaca cctccaaagg gtgttgcgct
     1921 caacgcaaca cccttgccta gaatagatct tttacgccga tttgtaagtt gttgtttttt
     1981 tagtgtgttt tttgtttacc tgttgcatcc agggtgacac aacgttgtcg catccaggat
     2041 gcgacaacac gcaacaaggt gttgcacaga tgtatcatga tggcttagat ttgaaccaac
     2101 gttagtgcat gggattttca gagggaaaaa tcatgtttat tgattcagaa aaacggctga
     2161 aacaactttc agatgaggca aagaaaagca ccgaggatct cgaagaagca aagaagaatt
     2221 caaggtttac acaggtatcc ccaaaaggtt gggaacgcgt tcgagagctg ctgaaggata
     2281 gccaaggcat atcagcactg aagctgtact catttttagc ggagcatatc gatccaacgt
     2341 gtggcgctgt cgttgcggat cagcaattcc tagctgaaaa acttggagtt agcagaagca
     2401 caattattcg gtggctcaat tacttagaat caaaaaatgc attagttaga atccccgttg
     2461 ctggtaaggt ttgtgcgtat gccctcgatc cacatgaagt ctggaaggga tacaacacta
     2521 cgaaaaacca tgcagcgttt gtcactaaaa cactggtcaa caaagacggt gatattcagc
     2581 gccgaatcat ggccatgttt tcaaattgag ccagcggcag gcggacaatc aggggctacg
     2641 tgttaacgtt ttgaccatga ttgtctatcc tgcattgctc ttttaccacc tagacagcac
     2701 catagtgata agataaacat gctcaaacgt tttatgtaac attagaaaaa ttcagttaat
     2761 taaaaaaggt tttctaaatg agc
//
LOCUS       DADLTD010000311         2740 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.315, whole genome shotgun sequence.
ACCESSION   DADLTD010000311 DADLTD010000000
VERSION     DADLTD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.315"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(229..2607)
                     /gene="ppsA"
                     /locus_tag="JN701_004036"
     CDS             complement(229..2607)
                     /gene="ppsA"
                     /locus_tag="JN701_004036"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY7366289.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNGRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          653 a          761 c          683 g          643 t
ORIGIN      
        1 agaggtggtg gcttttttta gccgtaaacg ggtcgcaaaa tataaatatc ctgaacatat
       61 cgtggtaatc gaaaaactac cgcgcactgc ctcaggtaaa atacaaaagt ttttgttaag
      121 aaaagatatt atgcggcgat taacgcagga tgtctgtgaa gagattgaat aagtttcatc
      181 ttcggggatc acataaaccc ggcgacaaaa cgccgccggg tatttatttt atttcttcag
      241 ttcagccagg cttaaccagg tttgcaccac ggtgtccgga ttcagagaca ggctatcgat
      301 cccctcttcc atcaaccatg cggcaaaatc ttcgtggtcg gacggaccct gaccgcaaat
      361 cccgacatat ttgccctgtt tcttcgcggc acggatagcc atcgacagca atgctttcac
      421 cgcatcgttg cgctcatcga acagttcaga caccacgccg gagtcacgat ccagaccgag
      481 cgccaactgc gtcatgtcgt ttgagccaat tgagaagccg tcgaaatatt cgaggaactg
      541 ctcggccagc aaggcgttgg acgggatttc acacatcatg atgattttca gcccgttctc
      601 accacgcttc agcccctgac gcgccagttc ttcaaccacc gctttcgcct ggtctacggt
      661 acgcacgaac gggatcatga tctcaacgtt ggtcagcccc atgtcgttgc gcacacgttt
      721 cactgcttca cactccagcg cgaagcagtc gcggaagctg tcggaaacat agcggcccgc
      781 gccacggaag ccgagcatcg ggttctcttc atctggctcg taacgctcac caccgaccag
      841 gttggcatat tcgttcgatt taaaatcaga gagacggaca atgacgcgct tcggataaaa
      901 cgcggcaccc agcgtcgcga tcccttcagt cagacgacca acgtaaaatt cacgcggaga
      961 atcaaaacct ttcatcatct cgcggatttc gttttgcaac tgcggttcct gatcgtcaaa
     1021 ctcaagcagt gcgcgtgggt ggacgccaat catacggttg atgataaatt ccagacgcgc
     1081 aagtcccacg ccttcgttcg gcagacaggc gaagtcgaaa gctcgatccg ggttaccgac
     1141 gttcatcatc actttcaacg gcagatccgg catggtttct acgctggagc ttttcacgct
     1201 aaattccagc aactccgcat agacgtaacc ggtatcacct tcggcacaag aaacagtgac
     1261 gttctcaccg tctttcatcc gttctgttgc atcaccacag cccacgaccg ccggaatacc
     1321 cagttcacga gcgatgatcg ccgcgtgaca ggtacgaccg ccacggttgg tgactatggc
     1381 agatgctttc ttcatgatcg gttcccagtc cgggtcggtc atgtcagtga ccagcacgtc
     1441 accaggttcg atgcggttca tttcgctgat gtcatggatg actttcaccg gacccgcacc
     1501 gatgcgatga ccgatagcac ggccttcggc gataatctta ccctgtgaat gcagcgtata
     1561 acgctccatg acctgaccgc gtgagcgcac ggtttccgga cgcgcctgca caatgaacag
     1621 tttgccggtg tggccatctt tcgcccactc aatatccatc gggcgaccgt agtgtttctc
     1681 aatttgtacg gcctgttttg ccagttcctg cacttcttcg ttggtcagcg agaagatgtc
     1741 acgctgttcc tgcggtacgt cttcgatttt aacctgcttg ccgtgctcct gggtcggcgc
     1801 gtaaaccatg cggatttttt tcgaccccat ggtgcggcgc acgatagccg ggcgattcgc
     1861 cgccagtgtc ggtttatgca cgtaaaactc atccgggtta accgccccct gcacgaccat
     1921 ctcaccaagg ccccatgcgg aagtgataaa caccacctgg tcaaagccgg attcggtatc
     1981 aatggagaac atcacgccag aagatgcgag gtcggagcgc accatccgct gaacaccggc
     2041 ggagagcgcc acgccacggt gatcgtaacc ctggtgcaca cgataagaga tggcgcgacc
     2101 gttaaacaga gaagcaaata catgtttcac tgccacgaga acggcgtcaa acccctgaac
     2161 gttgaggaag gtttcctgct gaccggcaaa ggaagcgtcc ggcatatctt ctgcggtggc
     2221 ggaggagcgc accgcaaaag aggcgttttc gtcatcggcg gaaagctgag cataggcttc
     2281 gcggatggcg ttttccagct caggctggaa gggagtgtcg ataatccact ggcggatttg
     2341 cgcgcccgct ttcgcaagct gagtaacatc gtcaatatcc gttttatcca gcagttcata
     2401 aatgcgctgg tttacgccgc tttggtccag aaactggtta aacgcgtcgg cggttgtggc
     2461 gaaaccattc ggaacggaaa cgcccattcc ggaaaggtta gtaatcattt cacccaggga
     2521 ggcatttttg cccccaaccc tgtctacatc attcatgccg agttggttgt accaaagcac
     2581 cagcggtgac gagccattgt tggacatcga acaatccttt tgtgataaat gaacggtttg
     2641 agaaacacat ttctgcgcat ttatctttgc atatttaacc ggatgaaaaa aacggtgaat
     2701 cgttcaagca aatatatttt tttacttttt aagactgatc
//
LOCUS       DADLTD010000312         2697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLTD010000312 DADLTD010000000
VERSION     DADLTD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.316"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            259..1674
                     /gene="gltX"
                     /locus_tag="JN701_004037"
     CDS             259..1674
                     /gene="gltX"
                     /locus_tag="JN701_004037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY7366290.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JN701_004038"
     CDS             complement(1725..2096)
                     /locus_tag="JN701_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7366291.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JN701_004039"
     CDS             complement(2119..2463)
                     /locus_tag="JN701_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7366292.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          665 a          657 c          672 g          703 t
ORIGIN      
        1 ccgctgtgtg gagtcgcatt atagggagag ttcaaaatga gtcaacgcat tttctaaaga
       61 aattgttcgt tcgtcgtaaa tttaagcaag atgatcgcaa aatagaccgt gttgcgcaat
      121 ttgtcaacga aaacaataat gcgtaaggta gaaacccgaa ctacattgag gaatcaggcg
      181 ggagtgatag aatatcgccc actcaatttt tccaggattt gccggttgtc ggcatctttc
      241 taaacgtaag gccatttcat gaaaatcaaa actcgcttcg cgccaagccc aacaggctat
      301 ctgcacgttg gcggcgcgcg tactgccctt tactcctggc tttttgcacg taaccacggc
      361 ggtgagttcg tgctgcgtat tgaagacacc gatcttgagc gttccacgcc ggaagctatc
      421 gaagccatta tggatggcat gaactggctg agcctggagt gggatgaagg tccgtactac
      481 cagaccaaac gttttgatcg ctacaacgcg gtgatcgatc agatgctgga agagggcact
      541 gcttataaat gctattgctc taaagagcgc ctggaagcgc tgcgcgaaga gcaaatggcg
      601 aaaggtgaga agccgcgtta tgacggtcgc tgccgccaca gccatgagca tcacgctgat
      661 gatgaaccgt gcgttgtacg ttttgctaac ccgcaggaag gttctgttgt ttttgacgat
      721 cagatccgtg gtccgatcga gttcagcaac caggaactgg acgatcttat tattcgccgt
      781 accgatggtt ccccaaccta taacttctgt gtggttgtcg atgactggga tatggaaatc
      841 acccacgtta tccgtggcga agaccatatc aacaacacgc cacgccagat caacattctt
      901 aaggccctga aagcgccggt gccggtttac gcgcacgttt ctatgatcaa cggcgatgac
      961 ggtaaaaaac tgtccaaacg tcacggggcg gtcagcgtaa tgcagtatcg tgatgacggt
     1021 tatttgccag aagcactgct gaactatctg gtgcgtctgg gctggtccca cggcgatcag
     1081 gaaatcttca ctcgtgaaga gatgatcaaa tacttcaccc tgaatgccgt cagcaaatct
     1141 gccagtgcgt tcaacaccga caagctgctg tggctgaacc atcactatat taacgcgctg
     1201 ccgccggagt atgttgctac tcacttacag tggcacattg agcaggaaaa tatcgatacc
     1261 cgtaacggcc cgcagctggc tgatctggtg aaactgctgg gcgaacgctg caagacgctg
     1321 aaagagatgg cacagagctg ccgttatttc tacgaagatt ttgctgagtt cgatgccgac
     1381 gccgcgaaaa aacatctgcg tccggtagcg cgtcagccgc tggaagtggt tcgtgacaaa
     1441 ctggccgcga ttactgactg gaccgctgaa aacgttcatc acgctattca ggcgacggcg
     1501 gatgagctgg aagtgggtat gggtaaagtt ggtatgccgc tgcgtgtcgc cgtaaccggt
     1561 gcggggcagt ctccggcact ggatgttacc gttcacgcaa ttggtaggac ccgcagtatt
     1621 gagcgtatca acaaagcgct ggcttttatt gctgaacgcg aaaaccagca gtaattagcg
     1681 cataaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtactccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLTD010000313         2679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLTD010000313 DADLTD010000000
VERSION     DADLTD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.317"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            231..770
                     /locus_tag="JN701_004040"
     CDS             231..770
                     /locus_tag="JN701_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7366293.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(996..1319)
                     /locus_tag="JN701_004041"
     CDS             complement(996..1319)
                     /locus_tag="JN701_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY7366294.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1603..1797
                     /locus_tag="JN701_004042"
     CDS             1603..1797
                     /locus_tag="JN701_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7366295.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          867 a          657 c          568 g          587 t
ORIGIN      
        1 aaaaaaaaac aataaaaaac accgcaaaaa tggattgtta acaaatgaaa atactcaatt
       61 cgaatctcat aacaaacata tcatcaacaa taagaaatca ccttctaaca tcatccaaaa
      121 atataaaaac acacccatca ccaaacatgg acgtaacaag tcatcgtcgt ttattccaga
      181 aagacggaat aggattgagc caaacaggaa aagcaacaca acttgtcatt atgtcgcatg
      241 gtggatggaa agagatctcc catccacaaa cactattcac gcgtcaaaaa ggcgatggat
      301 ggactgtagt ccccaaaaac ctaagaattg atttttatac aaaagacaac gacttcacaa
      361 agggactaag cgttctttca gaggtaaaca aacgccatac tgaagctcaa aaagggttaa
      421 caccatcact aaacataagc aaagatgacc taaaagttct agcgaacgcg cgcaatatac
      481 ctcaatcaaa attagaagaa gaaatgatgt cacaagccat atatagaaaa gaatacgcaa
      541 caagtaatga gaagataaaa aactatgctc tatattacca tgaacaagcc caaaatatta
      601 tacaaagaca tcaagatggc ttaggaaaca aaaatattga tatagcattt ataactgata
      661 aaaatcataa gaagcacctt tctgacatat ttaaagtaat aaatgaatca ggagtaaaat
      721 atgatgtaat tcactttggc gcttgccgag taaatagaga ggagaaataa tcatcattta
      781 ccataatccc ttaattgtac gcaccactga aacgcgctga acgcgattct gacgcagaca
      841 ccacaaaaat agatgtttta ccccggaaaa acaggaagga tgcgtcagga tcgttttcag
      901 ctcgctgcat agctatgcat gaaagcgaat ggagatcact ttgggagctt accgtgttca
      961 taccgtctgt tttcaacagt tttctccccg ggaagttaat ctgccataag cctggataac
     1021 agggcacggt gatactccgt aatagcgatc agcacctccg catactccct gtccccgata
     1081 cgcttgccgt cgataaagag ttttttctgt aacgccccca gtctcataac ttctttcagt
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaaccg
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga catgggtgtc ggggcaaagc cctgaccagg gcaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtcagc aagctcgaat
     1441 taccgacaaa ggcacggcag ttttttggca gttacagtgt tgtctgacag aaatgcccgt
     1501 caaaaagcca ggcggcagaa tagcggcagc aaaacagtcg aaaagtggta cgcgatataa
     1561 tcacataacg cagatgacag acagaacgga gaaaacgaaa agatgaacaa gcagcagcaa
     1621 gccgtactca acatggcggg attcataaaa agccagagcc tgacgctgct cgaaaaactg
     1681 gacgcactcg atgctgacga gcaggccacc atgtgtgaga aactacacga actcgcagaa
     1741 gaactccaga acagcataca gacacgcttt gaagtagaaa atagcacaga gagataacgc
     1801 tcaggacagg atgtcgaacg gcacaacaga gcgtaaggag aaaatgtcac ataaggcgct
     1861 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggtat
     1921 cagctcactc aaaggcggta atgcggttat ccacagaatc aggggataac gccggaaaga
     1981 acatgtgagc aaaaagccga aggcagggac aaaggccgcg caggtggcgt ttttccatag
     2041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2221 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2341 tgagtccaac ccggtaagac acgaattatc gccactggca gcagccactg ataaccgaat
     2401 tagaggagta agacttgaag tcatgcgcct gttaaggcta aactgaaaga acaagttttg
     2461 gtgactgcgc tcctccaagc cagttacctc ggctcaaaga gttggtatct ctgagaacct
     2521 tcgaaaaacc gccttgcagg gcggtttttt cgtttacagg cagtagatta cgcgcagaaa
     2581 aaaaggatct caagaagaac ctttaatttt ttttcacact tggtatccta ctttaaccca
     2641 taaaataaac acttacaccg acacgccctt aaacaaaag
//
LOCUS       DADLTD010000314         2653 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLTD010000314 DADLTD010000000
VERSION     DADLTD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.318"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            153..857
                     /locus_tag="JN701_004043"
     CDS             153..857
                     /locus_tag="JN701_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366296.1"
                     /translation="MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVING
                     SLGVGSTNALGGSSIVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAG
                     DGKKFTLSSSNNSTKNATFNLWGASTRPVVAELGNEAGWHFYSQRNTDNSVIFSVNGQ
                     IQPSNWGNFDSRYVKDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFR
                     PIQKYINGTWYNVAQV"
     gene            826..1389
                     /locus_tag="JN701_004044"
     CDS             826..1389
                     /locus_tag="JN701_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7366297.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1418..1951)
                     /locus_tag="JN701_004045"
     CDS             complement(1418..1951)
                     /locus_tag="JN701_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7366298.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1954..2511)
                     /locus_tag="JN701_004046"
     CDS             complement(1954..2511)
                     /locus_tag="JN701_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366299.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNSPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          757 a          567 c          587 g          742 t
ORIGIN      
        1 taaacagggc tggtaacgcc gtgcaaaaaa atggcgatac cttgtccggt gggcttactt
       61 ttgaaaatga ctcaatcctt gcctggattc gaaatactga ctgggcgaag attggattta
      121 aaaatgatgc cgatggtgac actgattcat acatgtggtt tgaaacaggc gacaacggca
      181 atgaatattt caaatggaga agccgccaga gcaccacaac aaaagacctg atgacgctga
      241 aatgggatgc actaaatatt cttgttaatg ctgtcattaa tggcagtctt ggagttggtt
      301 cgacgaatgc gttaggtggt agctctattg ttcttggtga taatgacacc ggatttaaac
      361 agaatggaga cggtattctg gatgtttatg ctaatagtca gcgtgtattc cgctttcaga
      421 atggagtggc tattgctttt aaaaatattc aggccgggga tggtaaaaaa ttcacgctat
      481 ccagctccaa caactccaca aagaatgcaa cgtttaattt atggggtgct tcaacccgtc
      541 cagtagttgc agagttaggc aatgaggcag gatggcattt ctatagccag cgaaatacag
      601 ataactcggt aatattttct gttaacggtc agatacagcc cagcaactgg gggaattttg
      661 attcacgcta tgtaaaagat gttcgcctgg gtacgcgtgt tgttcaattg atggcgcgtg
      721 gtggtcgtta tgaaaaagcc ggacacgcaa ttaccggatt aagaatcatt ggtgaagtag
      781 atggcgatga tgaagccatc ttcagaccaa tacaaaaata catcaatggc acatggtata
      841 acgtcgcaca ggtgtaaatt atgcagcata ttaaaaaata ttaagtccgg aaatccaaaa
      901 acaaaagaac aatatcagct aacaaagaat tttgatgtta tctggttatg gtccgaagac
      961 ggtaaaaact ggtatgagga agtaaataac tttcaggacg acaccataaa gattgtatac
     1021 gacgaaaata atattattgt tgccataacc aaagatgccc caacgcttaa tcccgaaggc
     1081 tttagtgtcg ttgagattcc agatataaca gccaatcgtc gtgccgatga ttcagggaag
     1141 tggatgttta aggacggaac tgtggttaag cggatttata cagcagacga acagcaacaa
     1201 caggccgaat cacaaaaggt cgcgttgctt tctgaagctg aaagcgttat tcagccactg
     1261 gaacgcgctg tcaggctgaa tatggcgatg gatgaggaac gcacacgact ggagtcatgg
     1321 gaacgctaca gcgttctggt cagccgtgtg gatacagcaa atcccgaatg gccacaaaag
     1381 cctgagtaaa aattaaggcc caatatcggg ccttctctca ttctggttgt tcgggaaacg
     1441 ttactggcag gctggaggtg tctgtagatt cgactttctg cgcatagagc atccactcgg
     1501 ttaatttttg tttattctcg tcggaaatga tgcccagccg tagctgtgag tcccatagct
     1561 gggttttatc cctgacaagt tgcaacaggc tttgcttttc attttccgct tgttgcctct
     1621 gctcttcctc ggtataagtt cgctttatca ctacgccatc tttgaacaac catttccccg
     1681 aaatatcagc ccggcgattt gctgtaatat caggtaattc aacgacgctt gcgccttccg
     1741 gattaattgc tgaaacatcc ttttcaatac aaataataac gccgttatgg taatagacca
     1801 ttttcaaagt gtctggctgg aaattctttt gttcctcata ccagtttttt ccatcatctg
     1861 aataaagcca tttgatgtta aattgctttg ttagctggta ttgctctttt gttttagggt
     1921 tgccagcagt aatgtttttt aagtgcatca tcgttaaata ctccccgcat tataccacgt
     1981 cccattaatg caatactgaa ttggccttgc ctgtgttgta tcaattaatt catcacggtt
     2041 tccgttaact gaacccgtaa cgacataacc tgacctgtca gaccagccgg gactattcca
     2101 tgtctgaaca gatgacagac cgccaaggcg aatacctgta ataaaccttg agttacattc
     2161 tgcccgcgta tatgcaccaa catctcccgc tgaaggtttt cgtgtcgtgg tatagaaatc
     2221 tgaccaacct aattcaaccc cataaccatc acgagccgac ctgtaggaaa tgccaccatt
     2281 tttataattc acacggaatt gtacggcagg acagctacca gcatttatat taaagtgaag
     2341 aattaatgca gatgcaccac caatcagggc gttatatgca ccactatccc aattccatcc
     2401 aacggatgta tcaccattaa cagtattacc tgtttgtcgg agtgcaaatg cattgacatt
     2461 tttggcacta attgaaatat cgctagaacc atcaaaacca actccagcaa tttttcgtgc
     2521 tgttttcagc tttgttgcag tagcggcatt gccggacagt tccccagaaa ggccaccgct
     2581 gaatgtttgt cgattggtcc aggtattcgc tgtactgagt aacggtattt tctccccgct
     2641 tgtgccgagt tct
//
LOCUS       DADLTD010000315         2611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLTD010000315 DADLTD010000000
VERSION     DADLTD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.319"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..2213
                     /locus_tag="JN701_004047"
     CDS             <1..2213
                     /locus_tag="JN701_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY7366300.1"
                     /translation="FSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNYILPGKLDAAR
                     NSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWENDNYSRYRPEKS
                     VWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLLLAFSHRPKET
                     FPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPLVFTKPSDNSE
                     AALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPARIPKAPDFWH
                     PAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQPQTLAAFAWD
                     LFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSARAVSGLNILTL
                     MNNDMALMQLHHVSQRAKSRPLRENAAEFLQVVAENRGLSQEELADRLVPTLGLDDPQ
                     ALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEALARLKGLKK
                     DATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGVYPANEPRCL
                     LKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLFADYEIMPPFR
                     QLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFVYDLGEYRLV
                     LKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSAPDVIFH"
     gene            2275..>2611
                     /locus_tag="JN701_004048"
     CDS             2275..>2611
                     /locus_tag="JN701_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366301.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDERKLRGESPEPVPVIFNCELQNIFNQ
                     LIEYYFIKVG"
BASE COUNT          658 a          631 c          655 g          667 t
ORIGIN      
        1 atttcagcac ggaatctctt gcgcaacgca tggaacaaaa aagtggacga gttgtattac
       61 aggaactggg ttttggggat gatgtatggc tgtttctgaa ttatatactc cccggaaaac
      121 tggatgctgc acgcaattca ctcattgttc agtggcatta ctaccagggg cgggttgaag
      181 agatcctgaa tggctggaac tccccggaag cacaattagc agaacaggcg ctccgcagcg
      241 gtcacataga agcgttaatt aacatatggg aaaatgacaa ctactcacgt tatcgtccgg
      301 aaaagagtgt ctggaacctg tatttattgg cacagttgcc gcgtgagatg gctttgacct
      361 tctggctgcg tatcaatgag aaaaagcatc tgttcgcggg tgaggactat tttctcagta
      421 tcctcggatt ggatgcgcta ccaggtctgc tgttggcttt ttcacatcgt ccaaaagaaa
      481 catttccgtt aattttaaat tttggcgcaa cagaactggc gctgcctgtt gcccgcgtct
      541 ggcgacgttt cgcggcgcag cgtgatctgg ctcgccagtg gattttacaa tggccggaac
      601 atacggctag tgcacttatc cctcttgtct ttaccaaacc cagcgataat agcgaagccg
      661 cattacttgc cctgcgttta ctgtacgaac agggacatgg cgaattgcta caaaccgtgg
      721 caaaccgctg gcagcgtaca gatgtatggt ctgccctgga gcagttgctt aaacagggtc
      781 caatggacat ttacccggca cgcattccaa aagcccctga tttctggcat ccggcaatgt
      841 ggtccaggcc tcgacttatc actaataatc agcctgttac cggtgacgct ctggaaatta
      901 tcggcgaaat gctgcgcttt acccaggggg gacgttttta tagcgggctg gaacaactga
      961 aaacgttctg ccagccacaa acgctggccg cttttgcctg ggatctcttc actgcctggc
     1021 aacaagcggg ggcccccgca aaagacaact gggcatttct ggcattaagt ctctttggtg
     1081 acgaaagcac ggcacgggat ctgacgacac agatcctcgc ctggccacaa gaaggcaaat
     1141 ctgcccgtgc tgtcagtggt ctgaatatac ttaccctgat gaataatgat atggcgctga
     1201 tgcagctgca tcatgtatca caacgggcga aatcacgccc cttacgtgaa aacgcagcgg
     1261 aatttcttca ggtggtcgca gaaaatcgcg ggctaagcca ggaagagtta gcggacagat
     1321 tagtcccaac cctgggcctt gatgatccgc aggcgttgag ttttgatttt ggtccccggc
     1381 agtttaccgt tcgcttcgat gaaaacctca acccggttat ctttgatcag caaaacgttc
     1441 gccagaaaag cgttccccgg ttgcgcgccg atgacgatca actgaaagcg cccgaggcac
     1501 tggcccgact aaaagggcta aaaaaagatg ctactcaggt gagcaagaac ctgctcccgc
     1561 gtcttgaagc tgccctacgt accacccgac gctggtcgct ggcagatttt cattctctgt
     1621 ttgttaatca tccctttacc cgtctggtta cccagcgatt aatatggggg gtttatccgg
     1681 caaatgaacc gcgttgttta ctcaaagcct ttcgtgtggc cgcagagggg gagttctgca
     1741 atgcgcaaga tgagccaatt gacctgcctg cggacgctct gattggcatt gcccacccgt
     1801 tagaaatgac agtagaaatg cgcagtgaat ttgcacagct ttttgccgat tacgaaatta
     1861 tgccgccttt tcgccagttg tcgcgccgca cggtgctgct cacacctgac gagtcaacca
     1921 gtaacagcct gactcgctgg gaaggtaaat ccgctaccgt tgggcagctt atgggaatgc
     1981 gatacaaagg ctgggagtca ggttatgagg acgcatttgt ctatgacctg ggtgagtacc
     2041 ggctggtcct taagttttca cccggtttta accactacaa tgttgatagc aaagcgctaa
     2101 tgagcttccg ttctcttcga gtgtaccgtg acaataaatc cgtcactttt gccgaacttg
     2161 atgtgtttga tttgagtgag gcgttaagcg cacctgacgt cattttccat taaaaacaca
     2221 gcgggcagtg atgcaactgc ccgttatcaa cacgacttac cagcggggaa agcgatgatc
     2281 gtgcaaaaag agctggttgc tatttacgat tatgaggtcc ctgtacctga aaatccgttt
     2341 tccttcagac ttgagatcca taaatgctct gaattattta caggttccgt ctatcgactg
     2401 gaacggttcc ggctacgtcc aacatttcat caacgtgatc gagaggatgt tgacccgcta
     2461 ataaatgatg cgttgattta tataagagat gagtttattg atgagcggaa attacgaggt
     2521 gaatcacctg aacctgtacc agtaattttt aattgtgaac tacagaatat attcaaccaa
     2581 ctaatagaat attactttat taaagtggga a
//
LOCUS       DADLTD010000316         2609 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLTD010000316 DADLTD010000000
VERSION     DADLTD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.320"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1838)
                     /locus_tag="JN701_004049"
     CDS             complement(<1..1838)
                     /locus_tag="JN701_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7366302.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSN"
     gene            complement(1882..2463)
                     /locus_tag="JN701_004050"
     CDS             complement(1882..2463)
                     /locus_tag="JN701_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7366303.1"
                     /translation="MKQQEEHNNKIDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
BASE COUNT          613 a          624 c          570 g          802 t
ORIGIN      
        1 atattggaag tgttattggc gatattggtg gtattcgtcg ccacagcatc gttggtggtt
       61 ttcagctgag agccgttaac agcatcagtg ctgtcagccg tcaggtcggc atctttaacg
      121 ttggtgattt tgctggtggt atcctggccg tgagctgcac tgaatgcctt tttagtttcg
      181 ttccagagca gggcatcagc ctgaaggtca ccaacggaat ccgtcaggtt ggtgatattg
      241 gtggtattgg tggcaatatt tgccgtattc gtggcaatgt tgctggtatt agtagcgata
      301 ttggcggtgt tggcttcgac atcgtcattg gtagctttca gttgggaacc gttaaccgca
      361 tccttactgg aggctgaaat cgtgccatcc gcaacatcgg tgattacgct ggcagttggg
      421 ctactagtac catgagcagc gctgtatttg cctttggttt ggtcccattg cagggtgttt
      481 tcatcaagta cagcgagcgc agcccctacg ttatttttgc taccattttt taaattgtag
      541 gttggtgctg taacagtacc gtcatcaaca tctactgcag cccctcctcc aagccttttt
      601 gctaccgagt cgctaatggc ataaagctgt gaaccattaa tggcatcgta actgtcagac
      661 ttgatcgcgc catttttcac attaacgatc ttgcgtttta atgaactatt gccgacagag
      721 acggtattgt ctaaatccgc aatggaatct gcgcccagcg cgacagagtt aataccgttg
      781 gcactgctat tactaccaag cgcaagactc ttatcaccag ttgcattact ggctgtgcct
      841 aaggcaatgg tgtttaagcc tctcgactgg ctaccattac ccagagcgat ggcatccacg
      901 ccatcagctt tgttgccctg gcctatggcg attgcattat ctgccagagc ctggctgcca
      961 tttcctaatg cgatagtatt gactttatcg gcaaggcttc cgaaccccat tgccatgcta
     1021 ttagtccctt ttgccttcgc attattgcca atagcagttg cattttcagc ggcttcggtc
     1081 tcagcaccta tcgcaatagc gttcgctgcg ctggcaagtg attgtctgcc aaatgcgagg
     1141 ctgtcagtac cgctagctac actgctacgc cccagggcaa ttgcgttttc ttcagatgcg
     1201 acgctacttg ctcccagcgc cattgaatac gcttttgacg cattggcggt gtcgccaaga
     1261 gcaatactca taatctcagt agctttagtg gcatttccca gggcaatggc gtttgctcct
     1321 tccgctttgg aagagtcccc catagcaata gacaatttac ccaatgcttt tgaataacgg
     1381 ccgagggcca ttgagtattg gcccaatgaa tacgaagagg cacccagtgc tatacttcta
     1441 tcgccttctg atattgcact aaccccaaag gccattgatg caccaccaat cgcatgggtt
     1501 tttgacccga tggcagagct atattggcct tcagctatag cgtcataacc cacagcagta
     1561 ctggaaccac tggtgttcat aaaagtattt gctttcgccc ctttgcctat agcaacccag
     1621 ccgtcgccag ctgatccact accgtaatca acaccctgac cgttgtcatt cccggcattc
     1681 gccaatgccc caaacgacga caacattcca cccgcaacca gcgcagaaat taacagctta
     1741 ctgcgcccag atttcttgcc acggcttttt gccgtttcgc tggtaacagt ataattccct
     1801 gtcgcagggt tccagataac tttaaatatt ttgttcatta atgatgctcg ctattccttg
     1861 ttataagtgt actaactgca tttatttttt gcttcttttc tgccgatttt cagcagaggg
     1921 aagattgtcg aactgaatat tttccatgtt gatggctaac ttcagatatc ccaggttatt
     1981 gggcgagaca cccttcaggg tcaaattaat gtcttgccat tgacctggta ataatttcaa
     2041 tgtggatttc attgatgtgt tgaaaagtaa ttgttgccac cgggcaaact ctttggcatc
     2101 gacaggtttt tcaccacccc atgtcgcatt aaaaccaata tttgagatag gtgtacttaa
     2161 taagttgacc agtgaaatat gaataacact gccattctct gtggcgtcaa tggataacac
     2221 tcgacccgct aaagccaccg aaccattgtt caggatgaaa tacttcgttt ttgctgggtt
     2281 aaactcggta aaaataaaag tgttttgttg atctttaacg gatttaatta cgtgagttaa
     2341 agtctttgta ctgcttatta tgccagtttg ttgtttttga atagtttcca gttgactctt
     2401 tagctgtgct tgttgttttt caagtagatc aattttattg ttatgttctt cttgttgttt
     2461 caataaacta ctttctggct tgccttcttc aatgtagtca catcccgtta atgcgcagag
     2521 aactgaaaca gtgacagcac tgaaaaaaat cttcttcaag ttcatgtaaa aatccttatt
     2581 tcctccgacc agcgatcaat gccggtaga
//
LOCUS       DADLTD010000317         2506 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLTD010000317 DADLTD010000000
VERSION     DADLTD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.321"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..323
                     /locus_tag="JN701_004051"
     CDS             <1..323
                     /locus_tag="JN701_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7366304.1"
                     /translation="APFYNGKSDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINRML
                     RFMFGKKRRAYVLNNGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESR
                     NEGQ"
     gene            310..858
                     /locus_tag="JN701_004052"
                     /pseudo
     CDS             310..858
                     /locus_tag="JN701_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1173..1694
                     /locus_tag="JN701_004053"
     CDS             1173..1694
                     /locus_tag="JN701_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366305.1"
                     /translation="MDVYANSQRVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFRAPSGHGLSGIAISDTGRDSADNVNGVYYRPLQ
                     KLINGTWYNVASV"
     gene            1754..2242
                     /locus_tag="JN701_004054"
     CDS             1754..2242
                     /locus_tag="JN701_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7366306.1"
                     /translation="MTKQYDVTWLFSKDGKNWYEELKNFASDTIKITYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSVITDKTSYNAIEWPASPEI
                     SS"
     gene            2266..>2506
                     /locus_tag="JN701_004055"
     CDS             2266..>2506
                     /locus_tag="JN701_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366307.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKPGWHNEYNVGDIAVYED"
BASE COUNT          659 a          554 c          682 g          611 t
ORIGIN      
        1 aggcaccgtt ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat
       61 accggcggct gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata
      121 ttaaccggat gctgagattt atgttcggaa aaaaacggcg agcttatgtt ctgaataatg
      181 gtgggctgag gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta
      241 tccagtcgtc gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg
      301 agtcccgtaa tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc
      361 ggagataaaa accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct
      421 gcgtatgatt caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg
      481 ccacacggca aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc
      541 caggctggcg gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca
      601 aaaggagcca ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat
      661 aacctgaccg atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg
      721 aaaaggatat tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggta
      781 cgggataatc ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa
      841 ggcgataaaa taaccggaga gctgaaaatc cgtggtgtta atgcgctgag gattttcaac
      901 gaagcttttg gcctgatttt tcgtcgttcg gaagagtgcc tgcacctatt cccaccagtg
      961 aaggtcaggg ggaaaatggc gatattggtc ccctgcgccc gttcaccatt aatttgcgga
     1021 cgggtgaaat atccatgtcg cataaagtgt ctgttggcgg tggttcgcag gtcaatggtg
     1081 cgctgggtat cggcgttcag aacgccctgg gggggaattc aattgttctt ggtgataatg
     1141 acaccggatt taaacagaat ggagacggta ttctggatgt ttatgctaat agtcagcgtg
     1201 tattccgctt tcagaatgga gtggctattg cttttaagaa tattcaggcc ggaactgcca
     1261 gaaaattcac gttatccagc gccaacaact ccacgaaaaa tgcagcgttt tatttgtggg
     1321 gtaatccatc caggtctgtt gttgcagagc ttggcgatga ttcaggctgg cattttttca
     1381 gccagaggaa tacagataac agcatcacgt tcgccgtaaa cggacaggta attccgttaa
     1441 attacggaaa cttcgatgcc cgctataaat atcgaacaga gggggtacag gatgtacggt
     1501 atggccatga aatgtattac agccccggca gtaacaccgt ttcgtggaga tttcgcgcac
     1561 cttcgggaca cgggctgtca gggatagcga tatcggatac cggccgtgac tcagcggata
     1621 acgtcaacgg cgtgtattac cgcccgcttc agaaactgat taatggcacc tggtataacg
     1681 tggcgagtgt ttaacaatgt tgcatttaaa aaatattact gcgggcaatc cgaaaaccgt
     1741 ggaacaatat cagctgacaa aacaatatga tgtcacctgg cttttttcga aagacggaaa
     1801 aaactggtat gaagagctga agaatttcgc cagcgacaca attaaaataa cttacaccgg
     1861 agacggtcgc gtggtgtggg tcggtaagga tgtgactggc attgaacccc gtaacgccag
     1921 tgttattgaa gttcctgata ttaccgccaa ccgccgcatt accgcgccgg gttactggtt
     1981 ttaccgcaat gatgaatttg tctttgacta cagactcaaa gcggaagatg agcgtgatgc
     2041 ccttctggct caggtcagtg cccggacagg ggaatgggaa gaagacctgc tgctggggct
     2101 aatcagcgac gaagaccggg aaaaactgaa agcgtaccgc atttacgcga aatcgctgca
     2161 ggcgatggat ttcagtgtca tcactgataa aacctcatac aacgccattg aatggcccgc
     2221 ctctccggaa atttcttcct gatttaattt atcgcgagaa aaactatgtc tgtagtgata
     2281 tcaggtgcgc tgactgatgg cgcaggtatc cccatgtccg gataccatat tattctgaaa
     2341 tcccgggtaa acaccccgga agtggtgatg cacactgttg ctgatgtgat gacaggaaac
     2401 gatggtgaat actgtttcca tgcgcggact ggaaaatatg gtgtgtatct gaaaccgggc
     2461 tggcacaacg agtacaacgt tggcgacatt gctgtatatg aggact
//
LOCUS       DADLTD010000318         2501 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.322, whole genome shotgun sequence.
ACCESSION   DADLTD010000318 DADLTD010000000
VERSION     DADLTD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.322"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..215
                     /locus_tag="JN701_004056"
     CDS             <1..215
                     /locus_tag="JN701_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7366308.1"
                     /translation="LSRQLTVEYDYVWFVPSGAVKDDLRHATLVAQPVPGHGAGEPIG
                     ILTRVDAMFSSGCQLMINAIRKSMPF"
     gene            complement(253..1866)
                     /gene="trg"
                     /locus_tag="JN701_004057"
                     /pseudo
     CDS             complement(253..1866)
                     /gene="trg"
                     /locus_tag="JN701_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            2265..2414
                     /gene="hokB"
                     /locus_tag="JN701_004058"
     CDS             2265..2414
                     /gene="hokB"
                     /locus_tag="JN701_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY7366309.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT          586 a          675 c          580 g          660 t
ORIGIN      
        1 cgctatctcg tcaacttacg gttgaatacg actacgtgtg gtttgtccct tctggcgcgg
       61 taaaagacga cctgcgtcat gccacgctgg tggcccagcc tgttccggga catggtgcag
      121 gcgaaccgat tggaatactg acccgcgtag atgcgatgtt ctcttctggt tgccagttga
      181 tgattaacgc tattcgaaaa tcaatgccgt tctgaaaggt gaagggatct gtcgatccct
      241 ccttgaacat tttcacaccg tagcgaaact aactggttca cccgctccgc gaggttctgc
      301 cgacacagaa tgtttgttca gacggaatac atccaccgcc tcagttaatc gtgccgcctg
      361 ttcttcaagg gacaccgccg ccgctgaggc ctcttctacc agagaagcat tctgttgcgt
      421 caccttatcc atttcagaaa tcgcctggct aacctgcgtt atgcctctac tttgttcatc
      481 cgaggcggcg gcgatttcct gcatgatatg tgtgacactc gcgacggcat caacaatagt
      541 gctcatggtt ttcccggccg ttgccacctc atccgacccc aggtcaatta acctgactga
      601 ttcactgatc aagccttcaa tctctttcgc cgcctgagcg ctgcgacttg cgagtgtccg
      661 tacttcgctg gcgacaacgg caaatccacg cccttgctca cccgctcgcg cggcttcaac
      721 ggcagcattc agtgccagaa tattcgtctg gaaagcaata ctgttgatga cggcggtgat
      781 ctcagaaatt ttcttcgaac tcgtggagat agcgcccatc gtttttactc cgtctgcccg
      841 ccatcgctgg ctttaataga agcctcttgc gccagtttgc tggcatgatg cgcgttatcc
      901 gcattctgtt tcaccgtcgc agtgagttgc tccatgctgg cggcagtttg ttcgatagcc
      961 gccgcttgtt cttcggtgcg agatgacagg tccgcattgc cagctgaaat ttcgctggtg
     1021 ccacgataaa tctcttccgc gccctgtcga acagtcccta ctgtcatccc cagtgaatgc
     1081 tgcatttgct gtaaatgacg acttaagcga ccgatttcat tacgacccgc cgattcatca
     1141 ttcatcgtca gatcgccact ggcgattttt tcaatccgtt gtgcggcatg ttgcagtgga
     1201 cgaatgacga tccgacgtag caccataaat gttatcagcg tcatgaccag ggcacgcaca
     1261 aacgcgccaa tcatgaacat cccacccagg cgggtgcgct gatgggccag ttccgccagt
     1321 tggttggctc tggtgctacg tatcttaacg gctttgttca aaatatcggt ataagcatta
     1381 tccagcggtc ggatctgctc actttcatga ttgataatcg cttcaaacat gccatttttg
     1441 gcatatttca acataggttg catacccgtg atataagcct gaaagcgttg atttaattca
     1501 gtgtcgaggg cttcatcagc aggtgttttc accggtcgat tctgataagc acgataacct
     1561 tgctgcgact gtttaatctc cgattcggct tgcgcaatat ttcgcttcat tgcttccatt
     1621 tccgcaatac gactcgcagc cccggcctga atcatattga tccgggctga acgcaaaaaa
     1681 tctgaactgt tcgctaaccc ggtccgaatc tcaatctccg cagtaacatc acgctgatcg
     1741 cgatcggcct gccagagcag aacttaatgc gaatagaact aagataccgc caagaatgca
     1801 ggcaaataac ggaaccagcc tgatgtgatg caaaaaacct aatcgctgtg agggagttgt
     1861 attcattacc gttgtctctc gtccaggttt actcctgata gaaagtcatc ggcacttgac
     1921 ggtaattact tatgaccaaa aacctctttg cgtcacattt ttacaacaat tattgcagag
     1981 aaaattaatg ctattggaat gaagagagta acgaatggct aaactggcta atccgaatga
     2041 cgaaaaattc tggaaaacag cacgcaattt cgtgaagtga ttattttcgt ccctaaggaa
     2101 acctcatagg atgcctgcgg gtgcctgaga ctttctgttt tcaggctaac cgggaatcag
     2161 aaagacgaag gccccgaccg atatttctat caaccgaggc caacgaatga acctagcaat
     2221 tcagatctta gcctcttacc cgccatccgg caaggagaaa ggctatgaag cacaaccctc
     2281 tggtggtgtg tctgctcatt atctgcatta cgattctgac attcacactc ctgacccgac
     2341 aaacgctcta cgaactgcgg ttccgggacg gtgataagga ggttgctgcg ctcatggcct
     2401 gcacgtccag gtaagggcaa gcgcggggat tttccccgcg catctgacta gcagattcat
     2461 tttctcatgg cacccctttt acacttcagt tagtgcatgt t
//
LOCUS       DADLTD010000319         2498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLTD010000319 DADLTD010000000
VERSION     DADLTD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.323"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            166..1302
                     /gene="potA"
                     /locus_tag="JN701_004059"
     CDS             166..1302
                     /gene="potA"
                     /locus_tag="JN701_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY7366310.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            1286..2149
                     /gene="potB"
                     /locus_tag="JN701_004060"
     CDS             1286..2149
                     /gene="potB"
                     /locus_tag="JN701_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY7366311.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(2381..>2498)
                     /locus_tag="JN701_004061"
     CDS             complement(2381..>2498)
                     /locus_tag="JN701_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7366312.1"
                     /translation="EHITSVQVAKFSVPLQVLLNRVDTSTAPDIEWPTSSAE"
BASE COUNT          607 a          608 c          625 g          658 t
ORIGIN      
        1 ataaaacact atcagtaagt tggagtcatt acctaaagct aagccagtaa ttcacaacaa
       61 agattggcga ttcaagcggt ttgccgtaga attaccgccc tcattaagag tcaccaaggt
      121 ggttaaccac aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa
      181 aaattgaata aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa
      241 tgctttgatg gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc
      301 ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt
      361 ctggaaactg ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg
      421 gcggaaaacc gctatgtgaa cactgttttc caaagctacg cacttttccc ccacatgacc
      481 gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg
      541 ccccgcgtga tggaagccct gcggatggtg caactggaaa ccttcgctca acgtaaaccg
      601 catcagctct ctggtggtca acagcaacgc gtcgccattg ctcgcgcggt ggttaacaag
      661 cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa
      721 atgcagaacg agctgaaagc gttacagcgt aagcttggca ttacattcgt cttcgtgact
      781 cacgaccagg aagaagcact caccatgtca gacaggattg tggtgatgcg cgagggtcgc
      841 attgagcaag acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc
      901 ggcttcattg gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag
      961 cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa
     1021 ccggggcaaa aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac
     1081 gacgacaacc acgctgaagg gcttattggt tacgttcgtg agcgtaacta caaaggcatg
     1141 acgctggagt cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc
     1201 aatgaagacg atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta
     1261 gaaagctggg aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat
     1321 tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg
     1381 caccagcttt ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cgctggataa
     1441 ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc
     1501 gctgatcgcc accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa
     1561 gctgccacac aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa
     1621 ctcattgatt cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga
     1681 gtttttgctc tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc
     1741 ggtgattatc ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag
     1801 tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt
     1861 acagactttt atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct
     1921 gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa
     1981 aaacctgctg atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt
     2041 tggtgcagcc accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg
     2101 gcgcgcttct cgtttgctga ataagaaggt gagcgaatta gatgattaat catttatatt
     2161 caatgcatta agtttgtttt tgagtggttt tgccgtttaa ggtaacggcg ttatagatcc
     2221 tatcaaaatc aatcaattcc attctggttt tggagaaaga tttttcgaga gcttcggtct
     2281 ttaactaaaa ctgattgcgt ataaccccaa tacccctccc cgactatctg aatggtcggg
     2341 gaggcacttc acgcacaaaa tatcatccct gcgccgttga ttactctgca gatgaagtcg
     2401 gccactcaat atcaggtgca gttgatgtat caacacggtt cagcaacacc tgaagtggca
     2461 cactgaattt ggccacctga acagaggtga tatgctca
//
LOCUS       DADLTD010000320         2445 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLTD010000320 DADLTD010000000
VERSION     DADLTD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.324"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..754)
                     /locus_tag="JN701_004062"
     CDS             complement(<1..754)
                     /locus_tag="JN701_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7366313.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLALLPHAHQQQNPPQEETVVSIAVDPESPA"
     gene            complement(756..1034)
                     /locus_tag="JN701_004063"
     CDS             complement(756..1034)
                     /locus_tag="JN701_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366314.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(1202..1414)
                     /locus_tag="JN701_004064"
     CDS             complement(1202..1414)
                     /locus_tag="JN701_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7366315.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(1617..1835)
                     /locus_tag="JN701_004065"
     CDS             complement(1617..1835)
                     /locus_tag="JN701_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366316.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(2009..2344)
                     /locus_tag="JN701_004066"
     CDS             complement(2009..2344)
                     /locus_tag="JN701_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366317.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
BASE COUNT          579 a          639 c          573 g          654 t
ORIGIN      
        1 gagccggaga ttccggatca acggcaatgc tgaccaccgt ctcttcctgt ggtggattct
       61 gttgctggtg ggcgtgaggc agcagcgcaa gattttttgt gcgctgcttc agtatgctgg
      121 tggcggtctg ctctcccggc acgatgtcgc tttcacggta cattgagcgg attttttccg
      181 cacgcaaccc cagcgaacga cgtaatactg cttccggtag cgcgtccgcc acctgattgc
      241 ggaccgccca ccaggataat tcagccagag ataattcacg ctcctgcgta ccgcttattg
      301 cgtgaccgat gacgtcaatc atccatgctg acaggttttg atgagcaagt tgctcgagtg
      361 attcggatgt ctggtcacgc aactggttgt cgcagtgcca gcacaacacc attgcgccgg
      421 taccataacg gtgaatgacg gtttcgctgt gatgataatc gccgtgtggc cactggcagg
      481 atttaatatg gcgcaacagc cagtcagaca atgcaccagc accaccagca gcacgaatca
      541 cccgtgcgtt actgaaaaac ggcagcaatg ttttgtcttc cactagcggc tggcgaacgg
      601 caggaacgac cccggacggc agattacgca tgcttttcgg ttccggctcc accagtaccc
      661 gggtattgtg gaataccggc atggattcac ggcctggctt aaggaccacc agcccgagtt
      721 ccggaaccag aacaggtcga agtaataccc gcacattacc tccagatccg ttgctggaat
      781 gtgcaggacg gacgcggtgg ccgttcggaa taagggagcc tgacgaagat tatccagtga
      841 cgataatcga gactgagggc tttcttaatc tcgtatccgt gtctgcggta gcgctgaatc
      901 agccattcag cctgttcttc ggtgcagggg tcgtgctgat accagtcaga tttgaatgca
      961 tgagaacgcc gcccgtgcct gctggcaggc gcggcagagt tatccgaatt gtaaaatttg
     1021 gtatcgtgcg ccatctgttt tctctgctgg cgcagcaggt gccagttgtt caggctgacg
     1081 gatggattgt aaaccagaac gaccagaaaa aacaaaaccc gccgaagcgg gttaagtgcg
     1141 ggtgcgttga ggatgcctga cacatcagcg gtggcgaggg atttctcccc cgccgggtct
     1201 cttactcctc aggttcgtaa gctgtgaaga cagcgacctc cgtctggccg gttcggattc
     1261 gtacctcgca gaggtctttc ctcgttacca gtgccgtcac tatgacggtt aaacagatga
     1321 cgataagggc gattaacatc gccttttgct gcttcatagc ctgcttctcc ttgcctttcg
     1381 gcacgtaaga ggctaaccta catgtgttca gcatggattg agcctcagat taatgttaag
     1441 cgtcttgcag gacgcgtagt gttaactggg gcttttctct atctgccttt tggtgttcat
     1501 gcctgagaca gatagcctca agcacccgca gtcattctac ttaactaaga tttccctgca
     1561 aaccgttttt gtccggcaca gtaaatatcc aactaaacca atagcgttcg ctgtatttac
     1621 cgccagtatt caatgcacat gaccgccatg aacaccccta aaaaaagggc atttatatgt
     1681 ccaaacatta atatcaaaac atcaattttt tccatatacc ttgctgtgaa gatgatgggc
     1741 atacatgatg cgaacaacca gaacgcaaca aacaaaaact gcaatgcgtt tttcattatt
     1801 ccccctacaa tcaatgtgca ataacattta aacacacctc aatttggccg gacatataaa
     1861 tatctaaacc agaaaaaatc acttacatag cgttacaaac tctttagtct aaatactcat
     1921 cgtaaaacat tccccatact tatcagtccg ttccgcgcca ggtagcttat tgccttatct
     1981 ggcaacctat aatcaggttt ccgctttttc agttggctgg tcgtttaacc gacatagtta
     2041 acccattaat ctggttgccg gatgctggtg gattttcgcg ttttagttgt tcataaaagt
     2101 gcacagcttt aaccagttct tctgatgtaa ccgggactgg tggggcagtg aataaggcct
     2161 gaatttcata gttcggcctg tcgttacaat cctctttttt cggtacatat ttccagtcac
     2221 caacccacaa ctccccctga gagtccataa cacctttttt cacgtagcga tatcgccacg
     2281 ctatcggctc tgcttccagc gatgcccgtg caattttgaa taactcgccc tctactcgcg
     2341 ccatccctga attggggtgg catttcgtaa tcgctatttt taatttggct tcttcgatta
     2401 attgttcttt tgttaattca gtcattttca ttaccgccct ttcag
//
LOCUS       DADLTD010000321         2419 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLTD010000321 DADLTD010000000
VERSION     DADLTD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.325"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(28..2217)
                     /locus_tag="JN701_004067"
     CDS             complement(28..2217)
                     /locus_tag="JN701_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAY7366318.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT          701 a          627 c          559 g          532 t
ORIGIN      
        1 gataattccc cggttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa
       61 atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc
      121 gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac
      181 aatcatcgca tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat
      241 gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc
      301 aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc
      361 gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata
      421 acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat
      481 taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa
      541 caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg
      601 cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc
      661 ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc
      721 taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat
      781 ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca
      841 gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc
      901 agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat
      961 gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc
     1021 actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca
     1081 aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag
     1141 attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa
     1201 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
     1261 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
     1321 aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
     1381 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
     1441 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
     1501 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
     1561 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
     1621 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
     1681 gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
     1741 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
     1801 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
     1861 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
     1921 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg
     1981 aaaattccac agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc
     2041 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
     2101 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
     2161 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
     2221 tacactactc tcactgatcg gagttttcag gcacacaaat aataagttta ttcgcatcgc
     2281 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
     2341 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
     2401 cgctttgcag cgggggttg
//
LOCUS       DADLTD010000322         2411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLTD010000322 DADLTD010000000
VERSION     DADLTD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.326"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..447
                     /gene="yeeE"
                     /locus_tag="JN701_004068"
     CDS             <1..447
                     /gene="yeeE"
                     /locus_tag="JN701_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7366319.1"
                     /translation="VLIGLIALLAWPLSEATGRMFGLGITSPTANILQFLVAGDVKYI
                     NWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGN
                     GLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            461..688
                     /locus_tag="JN701_004069"
     CDS             461..688
                     /locus_tag="JN701_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY7366320.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(731..2155)
                     /gene="sbcB"
                     /locus_tag="JN701_004070"
     CDS             complement(731..2155)
                     /gene="sbcB"
                     /locus_tag="JN701_004070"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY7366321.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          581 a          575 c          621 g          634 t
ORIGIN      
        1 gtactcatcg gtttgattgc gcttttagcc tggcccctga gtgaagcaac cggacgcatg
       61 tttgggttag gaatcacttc cccaacggcc aatattctgc aatttctggt cgcgggtgac
      121 gtgaaataca ttaactgggg cgttttcctg gtgttaggga tcttcgtggg gtcatttatt
      181 gcagccaaag cgagccgtga gttccgcgtt cgcgcagctg atgcacaaac aacattacgt
      241 agcgggttag gtggtgtact gatgggcttc ggtgccagta ttgcaggtgg ttgctctatc
      301 ggtaatggac tggttatgac tgcaatgatg acctggcagg gctggattgg ccttgtattt
      361 atgattctcg gagtctggac tgcatcctgg cttgtgtatg ttcgaccgca gcgtaaggcg
      421 cgactggcga ctgctgcggc aaattaagta aggtgtaaag atggcgatta aaaagctgga
      481 tgtggtgacg caggtctgtc cgtttccact catagaagca aaagcagcac tggcagaaat
      541 ggccagcggc gatgagttgg taattgagtt tgattgcacc caggcaactg aagcaatccc
      601 acagtgggcg gcagaagaag gacatgccat caccgattat cagcagattg gcgatgcagc
      661 gtggagcatc accgttcaaa aagcctgact caacattgtc ctccgccgta ccagcggcgg
      721 aggcttcaaa ttagacgatc tcttccgcgt actgccaaag tgcttttaac agcgccactt
      781 tttctttgtc atcggcatat tgttgtacca gcatctgcaa ttcatcagca taaccctgca
      841 aaaactctgg cgtgaatacc tggcggcggt gctccagcca gcgttgctgc tcggcataat
      901 ccagcgtccc cgggaagttg cgtgcccgat aattgaacaa cagcttttca atccgtttat
      961 caacaaaagt gatatccagt gccggtaaat tacgtggctc ggtttccagc acaattttca
     1021 ttgctgcacg atctgcgtca ctgaaaaagc cgttataaag ctgtgcatcc acgttatctg
     1081 taggcgtaaa cggttcggct tccgcgaata tcgccaccac tttttcacgc acttgcggat
     1141 tttcacgcag aattttcagg ttatcgaggc aatgctgacg attaattccc agtcggtcgg
     1201 catcttccgg gcgtagcgta ttcgcctggg ccagcaccgg acatttattg atgtgcacca
     1261 gcttaaccgg aacggcggcg ttatcgccaa gatcggtttt tgcggtatat aaacgctcgc
     1321 gcaatgtgtc gctatccagt tccagtaatg gcgaaatgtc tcctgccaaa tccaccataa
     1381 ttacagcatt gcgattttca ggatgccacg ccagcggtgc cacccagctg gtattgccgc
     1441 gccatgcccc aaacattccg gaaacgtgaa ccaggggggg catctgcgga acatcaatca
     1501 acgccatcag tttgtgttta ttacgatggg taaagagata atcaaacagg cgtggctgac
     1561 gcgtttttac cagtttcgcc atcgcaatag tggcgtacac atcagccatc gcatcgtggg
     1621 cgttgctatg ttcaatacca ttcgctttgg ttaaatgctc aaggcgaaag ctcggtagac
     1681 cgtcatcatt ttcaggccag tttattcctt ccgggcgcag ggcataacag gcacgcataa
     1741 catccaacaa atcccagcgc gagttatcat gctgccagct ccaggcgtaa ggatcgtaga
     1801 aattacgata aaaaacgttg cgtgtgactt cgtcgtcgaa acgcacattg ttgtagccca
     1861 gaatacaggt cttcggtacg gtaaaaagcg agtgaatacg ggcggcaaac gcggcttcgt
     1921 tttctccttt cgcccgtgct tcctgcgggg taataccggt aattaatact gctccaggct
     1981 ggggtaaata gtcatccgcg ggcttgcagt aaaagacttc gggttcgccg atgacattga
     2041 attcgctatc ggtgcgaatg gctgcgaact gtgcagggcg atctaacgcg gggtgcgtgc
     2101 caaaggtttc gtaatcgtga aagaggaagg taggttgttt atctgtgtct gtcattgtct
     2161 agcattgtcc actgtagtta attaactgtc tgttataact cgaaaaacaa ccattttttc
     2221 acctgagtat gtctacgaac gtcaaatata gttgtttact gtgcgctgtt gtccatgtga
     2281 tattgtgtac cagatgtgta ccaaaccatt atttctgagc gtaccaaata ttatttatgg
     2341 ctataagcga cacaaaactg cgtagtattt atggtaaacc atattcgggc ccacaagaag
     2401 tggctgatgc c
//
LOCUS       DADLTD010000323         2345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLTD010000323 DADLTD010000000
VERSION     DADLTD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.327"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..351)
                     /locus_tag="JN701_004071"
     CDS             complement(<1..351)
                     /locus_tag="JN701_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366322.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVN"
     gene            complement(1059..1520)
                     /locus_tag="JN701_004072"
     CDS             complement(1059..1520)
                     /locus_tag="JN701_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7366323.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(1532..2329)
                     /locus_tag="JN701_004073"
     CDS             complement(1532..2329)
                     /locus_tag="JN701_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY7366324.1"
                     /translation="MYHCDHRGLPLALISTEGATAWCAEYDEWSNLLSDENPHHLQQL
                     IRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVINVDPQG
                     LVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDG
                     NYKDGMTVWLFSCNTSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVL
                     TAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          654 a          504 c          414 g          773 t
ORIGIN      
        1 attaactgaa tttatatcaa tgattttttt attcttatct tttattttat ttactgtttc
       61 aatgcaatca gcggcaagtt ggagtggatt tagatttttc agatatggtc ttatgatctt
      121 tcccttactt aataaatagc atgtatagga ttcggaaagt ccagcatcta ttttgtacct
      181 cattatatta gataaggatg tgagaaattc cttgttatca ttcgcagcca tatatgccag
      241 tccgagttca gcctctgcgg ccttacaggc aaaatagctt agtgaacacg cttttcgttc
      301 ctctttagta ctggctgata ttagtgccca taatattgct ttgtctgcca aatctaactc
      361 tctctgaaat tgtcttgcag taactgcact cgcagaaata gagaaaaaaa tacaattgtt
      421 aataaccttg ccatattttt ttagtccgta actgttaatg tgctatctgg ctctgaaaat
      481 attaactcat ttaatttttg catgtcatta gataaaccag ttatacaacc ctcggaacag
      541 aatcgaggtc ctaaagacgg accaaaagcg tttaaacaac tatgtattga aaatccattg
      601 cgattataag ttcctgtatt aactgacggt actaatcgta tgccgtcagg ttttctattg
      661 cttgctcccg ggtcattaac attccaactc cataggcctt gaggtattgg ccatctattt
      721 ataaatgtgt acaacgggga ttatttttac attgcatatt attaccagaa gcgactggaa
      781 tactaagcac atcataaaaa ttatcatcac atggtatatc tgaaacacac gaaagcatac
      841 ctaaagttat gctgtataaa caggtggcta atcccaaggg gatcgattcc agaaatcggg
      901 ttcaacggat actgataaaa attccaccta ccttccagcc caatcggagc ctgagtgtta
      961 taccgcccct gctgcggtta atgttaccga taatagttat agtacagccc tgattcatca
     1021 tcatactact gctgcggcag tctgatgaac tattgctttt acggattctc cttattcttc
     1081 cttctatcca ttcgctttag ctttttgagc tcttgaagtt tgctctttgc atcaatatta
     1141 ccgttattta cagcgaattc catccaataa atagctctat tcaaattata atattctgat
     1201 aatgttggat ttgtatctga taaaaagacc ccataattaa actgcgctgt aacattcccc
     1261 tgagatgctg atctttccaa gaatcgcaat tgtttatcaa tattattttt agtaaagcag
     1321 taatattgat atagtcgaaa tgatgcctct gaatcaccat gttgtgattt atacttcagt
     1381 ttcatcagct tttcttcact taaatcatag gtcattgctg tagatcccat cacactttcg
     1441 ttttctttta ttttcacatc agcaccatgc aattgatcaa taagagaaaa ataaaaaaat
     1501 agtaacaagc atgctttcat acatctacaa cctatcttat atcactgggt ttttctggcg
     1561 taccttgcat attagaaatg gggtgcccag atggcccata tgttatccaa ttaccaaggt
     1621 cttttgttgt tatagccatt agatccttat ttgaattaag gttcgttggt gctgttagca
     1681 ctgtatccat ttttaactta ccattagttc ctcgccccca ccacgtccat agcgtgtcag
     1741 gtcctattac atttgtatgt aactctttag ctaattggct agcaaatgaa ttttgtcctt
     1801 tacttgtatt acaagaaaat aaccaaactg tcatcccatc tttataattc ccatcaaatt
     1861 taattagata tgctagatct ttagctgtca tgatactgcg cgttgcggat tcaatagatg
     1921 tgggggtacc atgccccccg attgtgaaaa cgcctgggat attaatctca tcagctacag
     1981 aatggataag atcactttcg gggtataaat ttatatcaac caaaccttgc ggatctacat
     2041 ttatgaccgg attcaacgga tactgataaa aattccatcc ccccttcagc ccaatcggat
     2101 cctgagtgat ataccgcccc tgcagcgggt cataatagcg gtggcggttg taatacaggc
     2161 cggactcctc atcatactgc tgacccggca ggcgaatgag ctgctgcaga tgatgcgggt
     2221 tctcatcact cagcaggttg ctccattcat catattctgc gcaccacgct gttgcccctt
     2281 ccgtgctgat gagcgccagc ggcaggccgc gatggtcgca gtggtacagg tggatttttc
     2341 gcgcc
//
LOCUS       DADLTD010000324         2343 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLTD010000324 DADLTD010000000
VERSION     DADLTD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.328"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(121..1167)
                     /gene="pyrC"
                     /locus_tag="JN701_004074"
     CDS             complement(121..1167)
                     /gene="pyrC"
                     /locus_tag="JN701_004074"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY7366325.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            complement(1273..1833)
                     /locus_tag="JN701_004075"
     CDS             complement(1273..1833)
                     /locus_tag="JN701_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7366326.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(1967..>2343)
                     /gene="grxB"
                     /locus_tag="JN701_004076"
     CDS             complement(1967..>2343)
                     /gene="grxB"
                     /locus_tag="JN701_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7366327.1"
                     /translation="KVNGYANKLLLPRFAKSAFDEFSTPAARKYFVDKKEASAGNFAD
                     LLAHSDGLIKNISDDLRALDKLIVKPNAVNGELSEDDIQLFPLLRNLTLVAGINWPSR
                     VADYRDNMAKQTQINLLSSMAI"
BASE COUNT          571 a          572 c          599 g          601 t
ORIGIN      
        1 ccagctggca atggagaagt tttcgctatg gtgacttcaa ttcgcataat agccccctgt
       61 tgaatatact ggttatttat acaggtaaaa taacctaatg acaacaggaa gctacgattt
      121 ttattgttta acggaccagc gtaccgtttc cccggcgagg aatggcacca gggtgtcatc
      181 agtcagtgcg atgctttcag caacctgttg ctcttcacgt accagttcga tgaatgtgtc
      241 gttgaccggc aagccataga actgcgggcc gtttacagaa cagaatgctt caaagtgctg
      301 cagagcattc atctcttcaa agacggtagc gtaactgccc agcgcggttg gggcgttgaa
      361 gcagcccgcg cagccgcaac tgctctcttt gcgatgacgt gcatgtggcg cagaatccgt
      421 accgaggaat actcgattaa aaccgctggc gaccagttca cgcaatgcct gttggtgaat
      481 attgcgtttg aggatgggta aacaatacag gtgcggacgc acgccaccaa ccagcatatg
      541 gttgcggtta aacatcagat gctgcggagt gatggtggca gccagccgtt catttccgtc
      601 acggacatag tcggcagcat ctttggtggt gatgtgctca aaaacgactt tcagcgcagt
      661 caggcgctgg cgcagaggtt ccatcacgct ttctataaag tgcgcttcac gatcaaaaat
      721 gtcgatatct gcatgtgtca cttcaccatg caccagtagc ggcataccga ttttttccat
      781 gcgctcaagt accggcatga ttgcgtcaat tgacgtcacg ccgtggctgg agttagtggt
      841 tgcgtttgcc ggataaagtt ttgcagcggt gaacacgcct tcgttaaatc cgcgctccag
      901 ctcattagga tccagcgaat ctgttaaata acaggtcatc aatggggtga aattgtgccc
      961 ggcaggtacg gcatcaagaa tacgctggcg atacgccacg gcagcctcaa cagtggtcac
     1021 gggcggagcc agattgggca ttacaatagc ccgtccataa atttcgctgg tatacggcac
     1081 gacagttttt aacatgtcgc catcgcggag gtgaaggtgc cagtcgtctg ggcggcggat
     1141 ctttaatacc tgggatggtg cagtcattaa tctctatgct ccggctgaag ggatgttttt
     1201 gccggacaca aaggataagc ggaaacgttt tcctttgcac gaaaaataaa gggcgcgaat
     1261 gcgccctcgt cattaatccg taaacggaat gacaatttcg cctggcttca cttcaatgcc
     1321 tttcgccagt tttttcgcca ttgcttcgcc ctgactgcca tcttcgcgca ggacgtaagc
     1381 aggttgctgg ttaaagtaat tgcgtaatgc ctggttcaaa tagggaagca acgtttgcat
     1441 caccgtttgc attttttccg gttgtaccgt cgcatcgacc acttccatct ctttcaggaa
     1501 gatcgcacct ttttctttat caaacacggg cagcgctttc agcttcagtt tcatggtcgc
     1561 tttctgacta ccgaacaggg agttcatgtc cagattggcg tctccggtta gggtaacctt
     1621 attcggctct tcgcgaccaa tttggctggt caagtttgtc agaacaatat gggcgtcagc
     1681 cacgccgggt aaaccgatat cttttgagaa attattatgt ttcgcaagcg actggttaat
     1741 ttcttgttcg gtgatggtgt attgggtgag ttgattacag ccaacgagca ggccactgac
     1801 gatcaatgca gcggcaaata aaaacttgtt catggtagtc ctcgacatga aatctgcgtc
     1861 aatatcctga cacaacgcag catgtgtcac cagcgataaa ctcgccagca gaaaaaactg
     1921 aaaacggcgg caacccgcga atacaggctg ccgcggcggg tcaggattaa atcgccattg
     1981 atgataacaa attgatttgt gtctgtttcg ccatattatc gcggtaatca gcaacgcggc
     2041 ttggccagtt gattccggct accagcgtca gattacgcag tagcggaaat agctgaatat
     2101 catcttccga aagttctcca ttcacggcgt tcggtttgac gatcagtttg tccagcgcac
     2161 gcaaatcatc gctgatattc ttaatcagac cgtcagagtg ggccagcagg tcggcaaaat
     2221 tacccgcgct ggcctctttc ttgtcgacga aatatttgcg cgcggcggga gtagaaaact
     2281 catcaaatgc cgatttggca aaacgcggca acagcagttt gttggcgtag ccattgacct
     2341 tgc
//
LOCUS       DADLTD010000325         2310 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.329, whole genome shotgun sequence.
ACCESSION   DADLTD010000325 DADLTD010000000
VERSION     DADLTD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.329"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..718)
                     /gene="ipaH7.8"
                     /locus_tag="JN701_004077"
     CDS             complement(<1..718)
                     /gene="ipaH7.8"
                     /locus_tag="JN701_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY7366328.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTR"
     gene            1047..1226
                     /locus_tag="JN701_004078"
                     /pseudo
     CDS             1047..1226
                     /locus_tag="JN701_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1243..1933)
                     /locus_tag="JN701_004079"
                     /pseudo
     CDS             complement(1243..1933)
                     /locus_tag="JN701_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1856..2107
                     /locus_tag="JN701_004080"
     CDS             1856..2107
                     /locus_tag="JN701_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366329.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(2127..>2310)
                     /locus_tag="JN701_004081"
     CDS             complement(2127..>2310)
                     /locus_tag="JN701_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY7366330.1"
                     /translation="IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT          672 a          437 c          521 g          680 t
ORIGIN      
        1 cgcgagtcga caatggattc cctgaaatat taatcctgta acttgaatct aattgtgaaa
       61 aactctccgg aagagtggtt atctgattaa aatgaaaata atattccttt tgtctgatat
      121 tgtttctatc agaaaattga ggaaaagtaa ccagattatt gcgctgagca cagagaattt
      181 ccagtttatc cggtaagtcg ggtaatgttg tcagcctgtt ataataaaca tgtaaaaatt
      241 tcaatttttc gggaagagcc ggtaaattat atagcctgtt attttcaaca aataatgatg
      301 ttaaatggtt tggcaaaaca ggaaggtttt ccagttgatt agaacgaaca cttaaagttg
      361 ttaatgactc aggaagttct ggtatcacag aaagcctgtt attatcagca ttaagttctt
      421 tgagaaatgc aggcagcttt ggcaatgatg ttaatttatt attttctaca atcaaagcag
      481 aaatatgctt tggtaattcg ggaagagacg tcaaatttag gtctgataaa tttaatactg
      541 cttcttgatt ctgaaagcac tgggagagtc tgttaaaagc aatgcctcgc tcttccccgg
      601 gagaagagtt cttttcccat tcagtcagga ttctcagata atgttcatta ctggttgagt
      661 ttgagttaat agagggggag caagaaactg atgagtgtgt attatttaca gagaacataa
      721 tgctagggca accttatatt tgtgagaata aatatttaaa attgtatatt tatacaaatt
      781 tcacttacca ttttcgatat taagttttaa tagtttccat tttacacata acagaagtta
      841 gttgtaattt atttgttgag gttttctatg gaattctttt tttattgtga ctgcagtcaa
      901 taataacact gaattcaaca ttccagtgag caaggaaatg taatccgagt aagtatattt
      961 gttttacatc atattttagc caagctcatt tattcctatt atagaatgat aaattaaaat
     1021 tactgtaaaa aaacgataca catttaatac gagggaggcc ggcagaaaaa cggcttgcgg
     1081 taaggcagga gaaatgtgtg ccgcgaatga agtccttgta tgactggata aagctccaga
     1141 ggaaaacttt gtcaaaccat gctgaaatgg taaaggcgtt caactatatc ctgaatcact
     1201 ggaatgcact gaatgagttt tgccgggtag tacttccaat cattagaata tgtgcttttc
     1261 aatgaaggaa ccgatgactt tttcgtggat ttccacggaa cgcgagaagc agattgtttt
     1321 acgtgccagc cgcttgatgc gggggcggag ggtcaggttg ttacgttcat acgttgcgtg
     1381 aagattttgc cggtcaggtg tttctcctcc ggcacttctc tggcgtagct gccccagtca
     1441 tcgctggtca ccatgccgat acagaacgga gtcaacaggg ctaacagttc ccgacaggtc
     1501 tcatcattcc gtggcccgca agtgtaagcc aacacaccgc cagttttagt gttgtacgca
     1561 taccagggcc agtgttgttg cgccttgctg ccaacgaagc gccactgctc atcaagttca
     1621 cagataaggg caacatcagc gtgagcgact ggcgcagatg ttatccgccg gggcgagagc
     1681 tttttaatgt ccgtatgacg gtattgatgc cgattttcag tgttctggcg gtataacagc
     1741 cccctgcgcc gttatgtgcc atttcaacga tctgctcctt aacgccgagt tttctggctt
     1801 cataagagta agtgagctgg aaaacacgtt tacatgagcg acaacggaaa cgctcatgcc
     1861 gggaacaact acggccatgt cgataaactt catcggaatt acagcgcgga caatgaacag
     1921 taacggtagt catggaaaac ctcaaatacc agtagattac cagaaacttc tatcgattga
     1981 agacattacc ttttgccgca ataggtgggg taacattggt gaaaacgcat tacgccgggt
     2041 ggcagttgga aaaaaattat cttttttcgg ctctgatcgt ggtggagaaa atgcggcaat
     2101 aatctaaata gctgcgctta ataccgctac acttttgcca gcccatgttt gcctccgggg
     2161 aatgggctga cggtttccat aaaatggcga acttttttca acagttgcca cattgagcgg
     2221 cactgatgat tacgcgttat tgtgtcgtga agtgcctgcc atagccgttc aacatgattc
     2281 acccatggcg agtaaaccgg ctgataaatg
//
LOCUS       DADLTD010000326         2276 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLTD010000326 DADLTD010000000
VERSION     DADLTD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.330"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..350)
                     /locus_tag="JN701_004082"
     CDS             complement(<1..350)
                     /locus_tag="JN701_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY7366331.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRN"
     gene            592..885
                     /locus_tag="JN701_004083"
     CDS             592..885
                     /locus_tag="JN701_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366332.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            957..1616
                     /locus_tag="JN701_004084"
     CDS             957..1616
                     /locus_tag="JN701_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY7366333.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1693..2154
                     /locus_tag="JN701_004085"
     CDS             1693..2154
                     /locus_tag="JN701_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY7366334.1"
                     /translation="MAEHLMNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          592 a          491 c          587 g          606 t
ORIGIN      
        1 tcgtttcggg tttgcagcac tcgcagctca tatttgccag aagcagggag tgagtattgt
       61 ccgtgactat cgagttctgg ggaatatctg gataaatcaa cagaatcatc gattcctggc
      121 ccgaagaggt aagtatcagc gccttcatta gagatactca catgtacttt ctgacctttt
      181 ttggcataga aggtatatgt atcgtaatcg tatcccttta tttcgccgga gtactgagcg
      241 ctgctatggc cttttctgaa ctcaacattg acattcttac ccgcagcaaa gctcgtggag
      301 gttagcagtg caaacagcaa tacagcctta ctgatgctct tgattttcat tggtagtctc
      361 ctgttcagaa tgagtacgat gaactgttat aatataacaa tccctaacgg gagaattcca
      421 gtggcttttc tgcgttcggt aaaatcccgg cgcggcattt tttgccgggg ggattactta
      481 taaaggggca tggactcaac aaaccgcaga aatctacagc tggctggctt gtggcgcggg
      541 tttcattgat gttagcttat gccaaaacct ggcatcttaa agagtaaaag tatgttttgt
      601 gtgatttatc gaagcagcaa gcgtgaccag acctatttat atgtcgaaaa aaaagacgat
      661 ttttcgcgtg ttcctgagga actgatgaaa ggttttggtc agcctcagtt agcgatgatt
      721 ctgccgttgg atgggcggaa gaaactggtc aatgctgata ttgaaaaggt taaactggca
      781 ttaaccgagc aaggttacta tttgcagtta ccgccaccac ccgaagattt gctgaagcaa
      841 catctttccg tcatggggca gaaaacagac gacacgaaca aataaccgat atccggcggt
      901 ggcattatct ttgtcggcgc gggttttcat atccacgata aggtgagggg aacgttatgt
      961 atcaacatca caactggcaa ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg
     1021 ttggcagtaa ctatgccaaa catattaaag agatgggcag cgcagtgccc gaagagccag
     1081 tgctgtttat taaaccagaa acggcactgt gcgatctgcg gcagccattg gcgatcccat
     1141 ccgatttcgg ttcagttcat catgaagtcg aactggcggt gttgattggt gcgacgctgc
     1201 gtcaggcaac ggaagagcat gtccgcaaag ccattgccgg ttacggcgtg gcgcttgatc
     1261 tgacattgcg tgatgttcag ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcta
     1321 aagcgtttga taactcttgt ccgctttccg ggtttatccc cgcggcggaa tttaccggcg
     1381 atccacaaaa tacaacgctg ggcctgagcg taaacggcga acaacgccag caaggtacga
     1441 ctgcggacat gatccataaa atcgttccgt tgatcgctta tatgagcaag ttttttaccc
     1501 tcaaggccgg tgacgttgtg ttgacaggca cgcctgatgg cgtcggcccg ttgcaaagcg
     1561 gtgatgagct gacagtcact ttcgatgggc attctttgac aactcgcgtt ttgtaatact
     1621 ttttgccgcc tgaaagcggc ggcaaaactt gcatcgctgt gccagactgg ttataaggtg
     1681 cgttttaacg taatggcgga acacctgatg aacgatgtac ctttctggca aagtaaaacc
     1741 ctggacgaaa tgagcgatgc ggaatgggag tcgttgtgtg atggttgcgg tcagtgttgc
     1801 ctgcataaac tgatggatga agacaccgac gaaatctact tcactaacgt cgcctgtcgc
     1861 cagctcaata ttaaaacctg tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc
     1921 gactgcatta aattaacccg tgaaaatctg ccaacattcg aatggctgcc aatgacctgc
     1981 gcttatcgtt tgctggcgga aggtaaagat ttacctgtgt ggcatccgct acttactggt
     2041 tcgaaagcgg caatgcatgg tgaacgtatc tctgtgcgtc atatcgcagt gaaagaatca
     2101 gaagtgattg actggcagga tcatatctta aataaacctg actgggcgca gtgataaatt
     2161 taatagatgt tgatgggggc tttcttaata tacgtctgga tgctcgccat ttcgtagccc
     2221 tgtttttgaa agcgctctgt cataatgaga gttacccgat accttattat caacag
//
LOCUS       DADLTD010000327         2250 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLTD010000327 DADLTD010000000
VERSION     DADLTD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.331"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            111..432
                     /locus_tag="JN701_004086"
                     /pseudo
     CDS             111..432
                     /locus_tag="JN701_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(466..672)
                     /locus_tag="JN701_004087"
     CDS             complement(466..672)
                     /locus_tag="JN701_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366335.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1160..1233)
                     /locus_tag="JN701_004088"
     tRNA            complement(1160..1233)
                     /locus_tag="JN701_004088"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1199..1201),aa:Gly,seq:ccc)
     gene            complement(1312..2067)
                     /locus_tag="JN701_004089"
     CDS             complement(1312..2067)
                     /locus_tag="JN701_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY7366336.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          600 a          528 c          488 g          634 t
ORIGIN      
        1 tcatacacta aatcagtaag ttggcagcat taccaatttt gctaagttaa tgcattaatc
       61 taaaaacgct ttaaatcaat acataaccat tatctcttgg gattatttac atgcgcaaaa
      121 gtttaatgct gttacttgct tcaacattga ttatttctgg atgtgcaaca aaaaaacaac
      181 tcgttccaac tggtggaagc aaatcagacg gcacagttag gatgagttat tcttacggta
      241 tgtttgaaag tcctgttgta gatccacaac aaggcatgaa tgcagccaaa gcgcgttgtg
      301 ccgcttaggg gatattcagg agccgagcct tttggtggat tcacttctca atgttcacaa
      361 ccttcttcat ctggttgtat gcaaacactt gtgactatcg aatatcaatg cactggcgat
      421 attaagaagt aataattagg ggcttccagc cccttctata accattcatg gaacagggac
      481 aaccgaaatc actttaaacc ctgaaacagc cgttactcca ttagatggag aattaaatct
      541 cacagtatct tttcttactg catcatgaat ttcatctttt gttggttcat gctctgaatg
      601 gaataaaacc tcttccattc taatttgatc accgtctcta tgctcataag taatcttgta
      661 gctaacagtc atatcaacct catgcagggg aattgtgaga ttattataga caatagaaac
      721 cataaagccc cgttggtgat acagcaacaa gcgggagcgt agcgacttca tcagattgtc
      781 agaaagatca caccttatca ggataaccgt gtacccgttg ggagcgtggc tgaaacgttg
      841 catatggttt gcaaggacca atatcaccat aaacctgttt acgcagccac aaggccgcac
      901 atcgggctta aatgcgtgtg ttcagtctgt cgatgacatt acaatgccag gatgggcttt
      961 atcttcacgt tgtgctatcg cggtgtgaga ggtgcaactg ccgccaacgt taggttatac
     1021 tgtcatctca ccgtgtaact taacgtgaca gttactaagg gcggatcaca atggtagaac
     1081 tcctaagcaa attaaatagg taactgttta agttgctgac agagaagact tttttagtct
     1141 tctccacctg gggagattct ggagcgggcg aagggaatcg aaccctcgta tagagcttgg
     1201 gaagctctcg ttctaccatt gaactacgcc cgcttcgaga tgcgtaaggc attataaacc
     1261 ttacgctctc cttagcaagt gccgcgctgc tgactgctga ttaattcgcc atcagcattt
     1321 tggcttgctg ccctgcggcg gcaagtaacg tagcggatca attgccgttg cacggtaacg
     1381 aatctggaaa tgcaggcgaa cagatgccgc atccgtgctc cccatagtgg cgattttttg
     1441 cccagtcttc acgctttgcc cattatttac cagcatcgtg tcattatggg cgtaagccgt
     1501 aatgtaatct tcactgtgtt taatcatgat gagattaccg tagccacgca gctggttgcc
     1561 cacatacacc acctttcctg cacccgcggc gtaaataggt gtaccccgtg gagctgagat
     1621 atcaatccct ttattgccgc catctgctgt cgaatacggc atgataactt tccctgtcgt
     1681 tggccataac caacaacgtt gccctactgg cggccaggaa gatttcggta ccgctgatga
     1741 cggtgtaacc gatgcggttt tggtcgttga tttggcggtt gatttacgtg tactactgct
     1801 acttttcgcc ccacccagtt ttagtttctg accaacttca atggtgtaag ggggggaaat
     1861 gccgttcagt cgcgccagtt cttttacgct ggttcccgtg gtgcgcgaaa tacgatatag
     1921 cgtatccccc cgtttcacgg tgtaaacgga gccggaatat gttcctgtat cggatgattt
     1981 gctacccgaa cagcccgcca aaagcagtcc aaccgataac aacatcacga tacccagaga
     2041 ttttttattc aggcgtcccg cactcaaaat ctttcctcgc ttaaaaaaca agacgcctta
     2101 tcatagcagc cagcctcttg atacccaatc tcttgcttct ggaaaaacgt ggtaatagag
     2161 aaagaggtta atttcatttg gcaaagggaa gtatcatcag caaaatattc gctgtagagg
     2221 tatcgaactt cctgactttt taccctattt
//
LOCUS       DADLTD010000328         2241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLTD010000328 DADLTD010000000
VERSION     DADLTD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.332"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            298..1950
                     /locus_tag="JN701_004090"
     CDS             298..1950
                     /locus_tag="JN701_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7366337.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFTR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(1937..2137)
                     /locus_tag="JN701_004091"
     CDS             complement(1937..2137)
                     /locus_tag="JN701_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7366338.1"
                     /translation="MTKKLAALQHQLSRKVKFSNNRQKQKHKIQQLHSHIANIRRDYF
                     HKVTATVSKKHVMIVNYVITAN"
BASE COUNT          697 a          414 c          522 g          608 t
ORIGIN      
        1 gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta tagaagggtg
       61 aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag accgtgacct
      121 gataaacctg aattgtgtcc tagacataaa gtcggtgaag ggaataaggc tcaggtcagc
      181 gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag cagcaactac
      241 tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac aggtacaatg
      301 ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact gacaggaaat
      361 cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca tacaaatgac
      421 caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc ccaaaaaact
      481 ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct attattatct
      541 ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga acccgatatt
      601 cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga atttgataat
      661 cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct ttctgctaac
      721 atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt cacccgtcag
      781 aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac aggaaattct
      841 gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag tgtcattgat
      901 gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat ggggcactat
      961 gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg cagtttgccg
     1021 actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg catggccagc
     1081 gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga taaacaaaat
     1141 ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc tgattttgaa
     1201 ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa tctcaaaatg
     1261 agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga aattactgaa
     1321 gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc acaactggat
     1381 gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt tcgttttgtt
     1441 cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga cgcagatttt
     1501 gctgttgtga gtggtgggga aattcgagat tccatagaga gtggtaacat cacatacaaa
     1561 gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt gacggggaaa
     1621 gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg agcctaccca
     1681 caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct aaaaataaaa
     1741 ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt taatgctact
     1801 ggtggggatg gctatccacg tattgataat agacccggat acataaatac cggatttatt
     1861 gatgctgaag tacttatcga atatatccgg gaacattcac cgctggatgc tgcttcatat
     1921 gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc acgtgttttt
     1981 tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg atatgggaat
     2041 gcagttgctg tattttgtgt ttctgcttct gcctgttgtt gctgaattta accttgcggc
     2101 ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa agtttattca
     2161 ttatggcatg atctgcgtaa gcggctcgtc agaaccgtat tgatatttac taagagctca
     2221 gatcaacttt ccagggcaac a
//
LOCUS       DADLTD010000329         2236 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLTD010000329 DADLTD010000000
VERSION     DADLTD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.333"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..505)
                     /locus_tag="JN701_004092"
     CDS             complement(<1..505)
                     /locus_tag="JN701_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7366339.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPIGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSISFANMDET
                     EFQQVYKS"
     gene            complement(544..831)
                     /locus_tag="JN701_004093"
     CDS             complement(544..831)
                     /locus_tag="JN701_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366340.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1081..1293)
                     /locus_tag="JN701_004094"
     CDS             complement(1081..1293)
                     /locus_tag="JN701_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7366341.1"
                     /translation="MLCTCRLASYMPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFVDYESRK"
     gene            1338..1521
                     /locus_tag="JN701_004095"
                     /pseudo
     CDS             1338..1521
                     /locus_tag="JN701_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303812.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1478..2143)
                     /locus_tag="JN701_004096"
     CDS             complement(1478..2143)
                     /locus_tag="JN701_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAY7366342.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          566 a          580 c          506 g          584 t
ORIGIN      
        1 cagatttata aacctgctga aactcggttt cgtccatatt cgcaaacgag atggatttcg
       61 ccctgcgccc acggctaccg tccggataaa tatgctcggt gtaaaatccg gcctgaatgg
      121 ttacccactc gcggaaagcc tcaaacgact ttagcaatgc tgtatctcgg gttctgcgtg
      181 tcgcaactgt attcagatat tgctctgcgg cttcgctcag agctggcgta tgttcccggc
      241 ctactgattc gcacaggtac tcaacgaagc ctgataccag ttctcgttcg cgaggcgtaa
      301 tcgccccacc gatcggagtc cagtaatcga atcccagttg caggagtttg aaaaaacgct
      361 tgtggaatgc gtagttacgc acacgcttaa agtctgcgtg tatccactca cctgttttga
      421 tttgatgcag aaaatcgcaa ctctccggcg tcgccgggag aagtaatccg gaagaggttt
      481 gtttgatcag ttgtatatgc gccatcggct ttctccggtg gcacggtgtt acacagcagg
      541 agttcaatcc tgctcaagat tgtagatgag tttattcttc agcaaaagca gaaaaccagc
      601 cttaaaacca atctctttca aaacccgtaa tgatgtgaca aattcgtcct cacgcaaaat
      661 aaaaccgtcc gtcagaagtc cattacaaaa ataaaataac acagcaccgc tcttcctttg
      721 ttgagattgc aaacatctaa tgcggcaatg gctgacaatc gctccattct caacgcgcac
      781 agcatagagg ccattttcac taaaaatttc acgcaattct tcgattttca tcttcagaat
      841 ccttccagat aaatagctct cccctgttcg gggtccatcc ctcttctccc tgcgcgctac
      901 ttaagtgagt cgattctatc tgcgaaggtg cgcgaatcaa attcaccgga aataaacaac
      961 aaaaaacccg ccgaaacgct gagagatcac agcgatgggc gggttaagtg cgggtgcgtt
     1021 gaggatgcct gacacatcag aggtgggcgg ggatgggatc agctccccgc ccagtcactc
     1081 ttacttcctg gattcgtagt ctacgaagac agcgacctcc gtctggccgg ttcggattcg
     1141 tacctcacag aggtctttcc tcgttaccag tgccgtcact atgacggtta aacagatgac
     1201 gatcagggcg attaacatcg ccttttgctg cttcatagcc tgcttctcct tgcctttcgg
     1261 catgtaagag gctaacctac atgtgcaaag catgaaattg gcctcagatt aatgttaagc
     1321 gtcttgccgg acgcgtaatg ttaactgggg cttttctcta tctgcctttt ggtgttcatg
     1381 cctgaagcag atagcctgaa gcagatagcc tcaagcaccc gcagtcattc tacttacctc
     1441 gccaatatga aatcaatcag aaaggtgccc cataaaatca ctccttctct tctttaccgt
     1501 agtggagttg accaattttg ataagagggc gtccctgaga tttgcggtgt agattggtat
     1561 cgcgcagaga atacacacag ccacaatatt cctgctgata gaatttttcg cgcttgctga
     1621 tttcaatcat acgggacgag cctccctgct tgcgccagtt ataatcccag tacaccatgc
     1681 ccggataatg cgcgacggct cgccgcccac agtcgttaac ctgctgcata tttttccagc
     1741 gtgaaatgcc cagtgaactg ctgatcacac tgaaaccatt ttcagcggcg tacagcgctg
     1801 tccgctcaaa acgcatgtca aaacacatgg tgcaacggat ccctcgttcg ggctcccatt
     1861 ccattccttt ggctcgttca aaccagttgt ctgtgtcgta atcagcatca ataaacggca
     1921 cgccgtgttg ttcagcaaag cgaatattct catccttacg aattaaatac tctttctgag
     1981 gatgaatgtt cgggttgtag aaaaagatgg tgtagtcgat tcccgaggcc tgaagcgcct
     2041 ccatcacctc accggaacat ggagcacagc aagagtgcag tagtagtttg tttgccccgt
     2101 ttgggagctc caatttgggc cgtttgaaat cagcaactgt cataaatatg tttattgggg
     2161 tcataaaaat atcaaagagt gtagcattag aacggggcta tcggaaacag atgtgtgact
     2221 gctccccgcc ctttcg
//
LOCUS       DADLTD010000330         2223 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLTD010000330 DADLTD010000000
VERSION     DADLTD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.334"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(24..2216)
                     /gene="pdeF"
                     /locus_tag="JN701_004097"
     CDS             complement(24..2216)
                     /gene="pdeF"
                     /locus_tag="JN701_004097"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7366343.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPFLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP"
BASE COUNT          666 a          530 c          498 g          529 t
ORIGIN      
        1 tcattcaacg tatcaattaa cggctacggc ttaccaataa gataaccctg catataatcg
       61 atccccaaag agagcaccgc ctcgcggatc tcttcgtttt caacgtactc tgccactacc
      121 cgcattttct tcattcgcgc caggtggcaa atcgatgcca ctatctgata atccagacta
      181 tttgacacaa tattgcggat aaaactgccg tcaattttaa gcagatcggc attcacattt
      241 ttaagccgtg catagctggc gtagccggtg ccgaaatcat caatcgcaat ctggcagcct
      301 aattcctgaa gatgctgcaa ggtgatttgc gcctgcttaa cattggtcag agcattactt
      361 tcggtgactt caaaaataag ttgccacgct tcaatctgat atttagccag caactgactg
      421 acttcaaaag gaaaacgagc ctgacatacc gaggttggag acagattaat agcaaaacgg
      481 tgagcgggca tcttcgctct gttttcagcc ataaattgca gcgtatgctc aatgacccac
      541 atgtcgatac tcgacgataa accaaattcg tgcgcgaccg gcaagaagct atcggggctg
      601 atcagttcat cattctcacc tttcatgcgc agaagaattt catggtaaac atcaccacgc
      661 ataccggtaa tcggctgggc catcaggaaa aaatggttgt gttccagcgc ctgctgtagt
      721 cgattcatca tcgcgacttt atctttcaat tcgcgttgca aatacattgc cccgcgacgc
      781 tgcatatttt ccggggcgtt ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc
      841 agcagcaggt agatatgatt cactggcgag cgcacatagc agtaactgac gccaatctgc
      901 ggttgcatcg gcatgccatc ccaaaagaaa cgaaattgct tgagatggct atccagtgcg
      961 gtaatgcgct cctggtgcga ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc
     1021 tgataaacat cttcacccgg ttccagcaag ggtgacagcc agtgagaaag tttttgcttg
     1081 tattgaatcc gcagcatgat gccatagttc ttaaccagca tttccatgcc agggatgcgt
     1141 aaataacaaa gcgcagacca gggggcatca cgcaacgcgc gattcagggc gcgaacattt
     1201 ggcagatgaa ccaccgggtc cacatacgca agccgctgaa tgcgtctgac taccgctctc
     1261 tgacgggttg ccagtacagc catataattg acaataaaag agaataccag ataactggag
     1321 gaggttatgg tcagctgcgt ggtatagcca ggataaatgg gaatgtaatt ttgatagctg
     1381 tggatgctga tcatcaacac gaccgcccag agcagagaaa tcagcttata accatagcgc
     1441 atcgctcccc acatcatcag gggcagcaat aatgacaagg tataattggt gctaaaaatt
     1501 gtgctttttt catttaacgg catgcataac agtaacaata aagcacctaa tgccagtagc
     1561 cagagcgcga actctttttt ggtgactttg gcatcaacct gctgttttaa ttgcgaataa
     1621 tagctacgta aataaaatgg atttcgcact acccgaatga tgaagtagca cagcgggaca
     1681 ccgatcagat tacccaccag taaggcctga taattgatta aagtcccgag gttaaaaggc
     1741 atgacaccga ccagattttc tctgctcgcc aataatccta caaatgcagc aaactggaaa
     1801 agtatcagaa acagcgttgc aggaaacaca atctgccaga atatacgctg ggaaattaaa
     1861 cgggtatcac catgtgaaac gttgttacgc cggggagtaa agaccctgta accgccccaa
     1921 cagagaatga taatgataat aaaattagcc gttaatgata gcgtttcgta aaaacccagc
     1981 tctgcatatt tgcggacaaa aatccctaag gcgatccctg gcaatgccgc ccagctgaaa
     2041 aacatcatca tgctgatcat cagtgccaga ggcagataaa agagaaaaac ctcaccggaa
     2101 gaaatatgcg cgaaagtatt aatgtgggcg aaaatgggca agattaaaaa aggcaggaac
     2161 agcggaagcc cccaccattt atcacgtatt tttatataag ttgcattcag tttcatagat
     2221 gct
//
LOCUS       DADLTD010000331         2174 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLTD010000331 DADLTD010000000
VERSION     DADLTD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.335"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            334..557
                     /locus_tag="JN701_004098"
                     /pseudo
     CDS             334..557
                     /locus_tag="JN701_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            666..934
                     /locus_tag="JN701_004099"
                     /pseudo
     CDS             666..934
                     /locus_tag="JN701_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1326..>2174
                     /locus_tag="JN701_004100"
     CDS             1326..>2174
                     /locus_tag="JN701_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7366344.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          726 a          464 c          365 g          619 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcaaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccgaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttcccaa aaaacaagaa aatatcatcc
      721 ctgaagtatc aaatactata ttagtatctt ctactgttac tacagatgga tgtactgttt
      781 ttaataaaca aaaacagcca tatacgaatt gcaaatacct gctaccaacc gaacgaaaaa
      841 gttgaaattc acagctacag aaatacaatg gttgacaaag ataaatgaag ccggaattga
      901 tgaaaaacaa agccaacggt attctgattt ctgaagttaa tttcagactc gtttaagctc
      961 cattttcaaa aaaaatatat cagggtttgt tactaataac ttctgaaaac agaaaaaaaa
     1021 tagagcagta attctatttc agagaggagt aactccatct atcctcgata cgtactgaac
     1081 gttatcgtga tatattcatc atgctttctt caccattatt cgagtacagg gagagacgca
     1141 atgaccttaa ataccttttt atggctgaat taccattctc aggaataaca gcagactcct
     1201 tcccatagac ggcttagggg gaaagcaata cccggagaaa gggtacaaag caaactgttt
     1261 taaaattaat aagctatatt ctatttcact agaattctta tcgctgataa gaaattttag
     1321 ttataatgat taaatcaacc aatatacagg caatcggttc tggtattatg catcaaataa
     1381 acaatatata ctcgttaact ccatttcctt tacctatgga actgactcca tcttgtaatg
     1441 aattttattt aaaagcctgg agtgaatggg aaaagaacgg taccccaggc gagcaacgca
     1501 atatcgcctt caataggctg aaaatatgtt tacaaaatca agaggcagaa ttaaatttat
     1561 ctgagttaga tttaaaaaca ttaccagatt taccgcctca gataacaaca ctggaaataa
     1621 gaaaaaacct attaacacat ctccctgatt taccaccaat gcttaaggta atacatgctc
     1681 aatttaatca actggaaagc ttacctgcct tacccgagac gttagaagag cttaatgcgg
     1741 gtgataacaa gataaaagaa ttaccatttc ttcctgaaaa tctaactcat ttacgggttc
     1801 ataataaccg attgcatatt ctgccactat tgccaccgga actaaaatta ctggtagttt
     1861 ctggaaacag attagacagc attcccccct ttccagataa gcttgaaggg ctggctatgg
     1921 ctaataattt tatagaacaa ctaccggaat taccttttag tatgaacagg gctgtgctaa
     1981 tgaataataa tctgacaaca cttccggaaa gtgtcctgag attagctcag aatgccttcg
     2041 taaatgttgc aggtaatcca ctgtctggcc ataccatgcg tacactacaa caaataacca
     2101 ccggaccaga ttattctggt cctcgaatat ttttctctat gggaaattct gccacaattt
     2161 ccgctccaga acac
//
LOCUS       DADLTD010000332         2026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.336, whole genome shotgun sequence.
ACCESSION   DADLTD010000332 DADLTD010000000
VERSION     DADLTD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.336"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..259
                     /locus_tag="JN701_004101"
     CDS             <1..259
                     /locus_tag="JN701_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366345.1"
                     /translation="HYPDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAE
                     LILNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(228..1952)
                     /gene="aceK"
                     /locus_tag="JN701_004102"
     CDS             complement(228..1952)
                     /gene="aceK"
                     /locus_tag="JN701_004102"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY7366346.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAALVEWLREILPGKTT
                     AELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVI
                     KDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQE
                     AAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIF
                     PGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSVSPGD
                     VFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRRQRFS
                     VRYGEMLF"
BASE COUNT          511 a          535 c          512 g          468 t
ORIGIN      
        1 gcattaccct gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg
       61 gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat ttggtcaccg
      121 ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac
      181 ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc
      241 tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc ttccacatgc
      301 ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc
      361 atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc
      421 gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga
      481 tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa
      541 tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca
      601 ggaaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg
      661 cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctcg
      721 atatagagat ggcgaattac aatctgctca ccaagatcgg tgattttttc cgctgcttcc
      781 tggagcagta attccattaa tgccggggaa atatgccgct tctccagcac aaagttttca
      841 aactcctggg tgtccgccat tcggcccaca cggtcgtgct ctttcaccag ttgatagcag
      901 gcacgaacgt gagcggcaga catctctttc tgcggcgcga acttgtcttt gatgactttg
      961 aatacccgat caaagcccgg cagcgtaaac accaacatca ccattccacg aatacccggc
     1021 gcttcaatga actgctcatt acagccctgt agataaacga gatattcgcg gtagctttcg
     1081 gttttggcgt gcttctggca gccgatagcc atatacaatt cagcagtggt tttacctggc
     1141 agaatttccc gtagccactc gaccagcgct gcataaacca taaaataaga acgcgcaaag
     1201 ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg
     1261 tcgtccgtct ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg
     1321 cccaccagcc aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt
     1381 tccgcgaggt tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca
     1441 cggcttttat tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga
     1501 gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag
     1561 cggcgctctg gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca
     1621 aataaccgac agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc
     1681 ggcaacagcc gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg
     1741 ccgttagtaa tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg
     1801 atacggtttt tcatcgccag ctggacagca tgccagtcgg cctgttcgaa acgctgctgc
     1861 gccccggaag tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt
     1921 tgagcaatca gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt
     1981 gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg caacgg
//
LOCUS       DADLTD010000333         1983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLTD010000333 DADLTD010000000
VERSION     DADLTD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.337"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>1983
                     /locus_tag="JN701_004103"
     CDS             <1..>1983
                     /locus_tag="JN701_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY7366347.1"
                     /translation="ETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRL
                     QLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQAL
                     PEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQ
                     TFHREAAGEFSGEITGVTDGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDTLP
                     GYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSF
                     TYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLN
                     RREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGL
                     ITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRY
                     DNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEG
                     LSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRT
                     TQGELTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGK
                     LIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQWQY"
BASE COUNT          442 a          597 c          625 g          319 t
ORIGIN      
        1 gtgaaaccga catcgccctg cccggcccgc tgccgttcat cctctcccgc acctacagca
       61 gttaccggac aaaaacgccc gcgccggtgg ggagcctcgg ccccggctgg aaaatgcctg
      121 cggatatccg cttacagctg cgcgataaca cactgatact cagtgataac ggcggcagaa
      181 gcctgtattt tgagcacctg tttcccggtg aggacggtta cagccgcagc gagtcactgt
      241 ggctggtgcg cggcggcgtg gcgaaactgg atgaaggtca ccggctggcc gcactctggc
      301 aggcgctgcc ggaagaactc cgcttaagtc cgcatcgtta tctggcgaca aacagtccgc
      361 aggggccgtg gtggctgctc ggctggtgtg agcgggtgcc ggaagcggat gaggtgctgc
      421 ctgcgccgct gccgccgtac cgggtactga ccgggctggt ggaccgcttc gggcgcacac
      481 agacgttcca ccgcgaagcc gccggtgaat tcagcggcga aatcaccggc gtgacggatg
      541 gtgccgggcg tcacttccaa ctggtactga ccacgcaggc gcagcgggca gaagaagccc
      601 ggcagcaggc catttccggc gggacggaac cgtccgcttt tcctgatacc ctgccgggtt
      661 acaccgaata tggccgggac aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc
      721 cggaataccc ggagaattta cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg
      781 gcgaactggc ggcagtgtat gaccgcagca atacacaggt gcgtagcttt acttacgatg
      841 ataaataccg gggccggatg gtggcgcacc gtcacacggg ccggccggaa atccgttacc
      901 gttacgacag cgacgggcgg gtgacagaac agcttaaccc ggcaggctta agctacacgt
      961 atcagtatga gaaggaccgc atcaccatca ccgacagcct gaaccgccgt gaagtgctgc
     1021 acacgcaggg tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg
     1081 tcacgcagag tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc
     1141 ggacaacaga atacagcccg gatgtggtga cgggcctcat cacgcgcatc accacgccgg
     1201 atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc
     1261 ctgacgggct ggaaatacgc cgggaatatg atgaatgggg ccgtctgatt caggaaactg
     1321 cccctgacgg cgatatcatc cgctaccgtt atgataatcc acacagtgac ttaccctgcg
     1381 caacggaaga tgccaccggt agccggaaaa ccatgacgtg gagccgttac ggtcagttgc
     1441 tgagcttcac cgactgttcc ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga
     1501 tgacggcggt gcaccgcgag gaagggctga gtcagtaccg cgcatacgac agccgtggac
     1561 agttaattgc cgtgaaagac acgcagggcc atgaaacgcg gtatgaatac aacgccgccg
     1621 gtgacctgac cgccgtcatt gccccggacg gcagcagaaa cgggacacag tacgatgcgt
     1681 ggggaaaggc catccgtacc acgcagggcg agctgacgcg cagtatggaa tacgatgctg
     1741 caggacgggt catccgcctg accagtgaaa acggcagcca caccaccttc cgttacgatg
     1801 tactcgaccg gctgatacag gaaaccggct ttgacggccg cacacagcgt tatcaccacg
     1861 acctgaccgg caaacttatc cgcagcgagg atgaggggct ggtcacccac tggcactatg
     1921 acgaagcaga ccgcctcacg caccgcaccg tgaatggcga aaccgcagag cagtggcagt
     1981 atg
//
LOCUS       DADLTD010000334         1963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLTD010000334 DADLTD010000000
VERSION     DADLTD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.338"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="JN701_004104"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="JN701_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY7366348.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1098..1508)
                     /locus_tag="JN701_004105"
     CDS             complement(1098..1508)
                     /locus_tag="JN701_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7366349.1"
                     /translation="MPFHRPTKWVQYISAAPALSQQAVDQEWSYMDFLEHLLHEEKLA
                     RHQRKQAMYTRMAAFPAVKTFEEYDFTFATRAPQKQLQSLRSLSFIERNENIVLLGPS
                     GVGKTHLAIAMGYEAFKIFYDISKISLELYHNIH"
     gene            1519..1963
                     /locus_tag="JN701_004106"
                     /pseudo
     CDS             1519..1963
                     /locus_tag="JN701_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          618 a          351 c          394 g          600 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa atgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag
     1921 cattggagct gtcaaaagtg aagtggcaca ctgaatttgg cca
//
LOCUS       DADLTD010000335         1961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLTD010000335 DADLTD010000000
VERSION     DADLTD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.339"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..819
                     /gene="bluF"
                     /locus_tag="JN701_004107"
     CDS             <1..819
                     /gene="bluF"
                     /locus_tag="JN701_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY7366350.1"
                     /translation="FVLATEQSTYFEIPAEDSWLFIADGSDKELDSCALSPTINDHFA
                     FHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALE
                     LGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEFTESEVISRFDEFAEA
                     IKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAII
                     KCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWPEKK"
     gene            1024..1755
                     /locus_tag="JN701_004108"
     CDS             1024..1755
                     /locus_tag="JN701_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7366351.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          563 a          437 c          434 g          527 t
ORIGIN      
        1 tttgtccttg caaccgaaca atcaacctat ttcgagatcc ctgccgaaga ctcctggctt
       61 tttatcgctg acggatctga taaagaactt gattcctgtg ccctttcacc aactataaac
      121 gaccactttg cctttcatcc tattgtcgat cccttatcgc ggcggataat cgcttttgaa
      181 gccattgtgc aaaaaaatga agatagccca tcagccatag cggttgggca gcgtaaagac
      241 ggggaaatct acacagcgga tctcaaaagt aaggcgcttg cattcacgat ggcacacgca
      301 cttgagctcg gtgataaaat gatttcaatc aatctattac ctatgaccct agttaacgaa
      361 cctgacgcag tctctttttt acttaatgaa ataaaggcca atgctctggt gcctgaacaa
      421 atcatcgttg aatttactga aagtgaagtc atatctcggt ttgatgagtt tgccgaagcg
      481 attaaatcgc taaaggctgc tggtatcagt gtagcaattg atcattttgg cgcaggtttt
      541 gctggtttgt tactcctgtc acgcttccag cctgacagaa ttaaaatcag tcaggaattg
      601 attaccaatg ttcataaaag tgggccacgg caggcaatta ttcaggcgat cataaaatgc
      661 tgtacatcac ttgaaattca agtcagtgct atgggcgtgg caacaccaga agagtggatg
      721 tggcttgaat ccgcaggaat tgagatgttt cagggagatc tgtttgctaa agctaaattg
      781 aatggtatcc cttccatagc gtggccagag aaaaaataat tttcagcaca ttctttcaca
      841 tgatttcagt aaatcatcgt gaaaatgaat acgttacgtt taaatttaat ccacattaaa
      901 aataatgttt catccaattt agctgaaatc ttatttatac tttgtacaat tttagcgtat
      961 attgtacaaa gtattattgg gtcgtgtaca ggcgacggag atttgtgacc gcaaggagga
     1021 attgtggctt attacagcat tggtgatgtt gctgaacgtt gcgggattaa tcctgtcact
     1081 ctccgggcct ggcaacgccg ctacggtttg ttaaaaccac agcgcagtga aggcggacac
     1141 cgactctttg atgaagaaga catacaacgc atcgaagaga tcaagcgttg gataagtaat
     1201 ggcgtccctg taggcaaagt taaagcatta ctggaaacca ccagccagga tacggaagat
     1261 gactggagcc gcctgcaaga agagatgatg tcaattcttc gcatggctaa tcctgccaaa
     1321 ctacgcgcga gaattatttc actgggtcga gagtacccag tagatcaatt gattaatcat
     1381 gtttaccttc ctgttcgcca gcgtctcgtg cttgatcaca acacctcccg cattatgagc
     1441 agtatgtttg acggcgcatt aattgaatac gcagcaacct cgcttttcga aatgcgccgt
     1501 aagcccggta aagaagccat tctgatggcg tggaatgttg aagagagagc acgattgtgg
     1561 ctggaagcat ggcgtttatc attgtcagga tggcacattt ctgttcttgc tgatcccatt
     1621 gaatcgccgc gcccggaact gttcccgacg caaacattga ttgtctggac aggcatggca
     1681 ccaacgagaa ggcaaaacga acttttacaa cactggggtg agcaaggtta taaagtcatc
     1741 tttcatgccc cctaatttca cctttatccc gccccttaga cagaatgtta taatctgaac
     1801 ccacaacagc atttgtaccg gagctgatcc gttgcaaaat gctcaccggg agaattcagg
     1861 taatccctct atcacactct gtacatatca cagccaaagg acagaccaga ttgaagtggt
     1921 aaaaccgatc gtgcacacta cgcagtctca caactaacaa g
//
LOCUS       DADLTD010000336         1945 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLTD010000336 DADLTD010000000
VERSION     DADLTD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.340"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..954
                     /gene="tuf"
                     /locus_tag="JN701_004109"
     CDS             <1..954
                     /gene="tuf"
                     /locus_tag="JN701_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7366352.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            1137..1331
                     /gene="bfd"
                     /locus_tag="JN701_004110"
     CDS             1137..1331
                     /gene="bfd"
                     /locus_tag="JN701_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY7366353.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1404..1880
                     /gene="bfr"
                     /locus_tag="JN701_004111"
     CDS             1404..1880
                     /gene="bfr"
                     /locus_tag="JN701_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY7366354.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1877..>1945)
                     /locus_tag="JN701_004112"
     CDS             complement(1877..>1945)
                     /locus_tag="JN701_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY7366355.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          514 a          440 c          504 g          487 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccgtaccgt tggcgcgggc gttgttgcta aagttctggg ttaattacac
      961 gttaattagt tttgaattga aaagggcgct tcggcgccct ttttgctgcc cgtaatttga
     1021 gctcgaaata tttagtcgta attttgctcg ctgaactatg gcgcaaataa aactgtgcta
     1081 ttgtaatcat aatcattctc atttacactt tgcgcagaat ttgaacggga gcaatcatgt
     1141 acgtttgttt gtgtaatggt gtaagtgaca aaaaaattcg ccaggcagtc cgtcagtttc
     1201 atccgcaatc tttccaacaa ttgcgtaagt ttattcctgt gggaaatcaa tgcggtaagt
     1261 gcattcgcgc cgcgcgcgaa gtgatgcagg acgagttgat gcagatgcca gaatttaaag
     1321 agattgcctg aagaccacgc ttattttttg acatcccgct agccccatct acgctctaat
     1381 agtggaagcg gagggactat aaaatgaaag gtgatgttaa aatcataaat tatctcaata
     1441 aactattggg aaatgagctt gtcgcaatca atcagtattt tctccatgcc cggatgttta
     1501 aaaactgggg cctgatgcgt ctcaatgacg tcgaatacca tgaatccatc gatgagatga
     1561 aacacgctga taaatatatc gagcgtattt tgtttttaga aggaaccccc aacttacagg
     1621 atttaggcaa actggggatt ggtgaagatg tcgaggagat gctgcaatcc gacctgcgat
     1681 tagaactgga aggcgcgaag gatctgcgtg aggctatcgc atatgccgac agcgttcatg
     1741 actatgttag ccgcgatatg atgatcgaga tccttgctga tgaagaaggt catatcgact
     1801 ggctcgaaac tgaactggat ctcatcggta aaattggcct acaaaattac ctgcaatcac
     1861 aaattaaagt taaagattaa cggcctatca gcaaactatc ccagcctata acgaaacccg
     1921 cagccgccag aaatggccca aaagg
//
LOCUS       DADLTD010000337         1932 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLTD010000337 DADLTD010000000
VERSION     DADLTD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.341"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..255
                     /locus_tag="JN701_004113"
     CDS             <1..255
                     /locus_tag="JN701_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7366356.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /locus_tag="JN701_004114"
     CDS             complement(305..1561)
                     /locus_tag="JN701_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY7366357.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..>1932)
                     /gene="mltC"
                     /locus_tag="JN701_004115"
     CDS             complement(1763..>1932)
                     /gene="mltC"
                     /locus_tag="JN701_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708734.1"
                     /note="Murein hydrolase C; membrane-bound; lytic;
                     catalyzes the cleavage of the beta-1,4-glycosidic bond
                     between N-acetylmuramic acid and N-acetylglucosamine
                     residues; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7366358.1"
                     /translation="SVLRVFSNDKIQAANIINTMTPGDVYQTLTTRHPSAESRRYLYK
                     VNTAQKSYRRR"
BASE COUNT          527 a          440 c          515 g          450 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gc
//
LOCUS       DADLTD010000338         1894 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLTD010000338 DADLTD010000000
VERSION     DADLTD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.342"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(495..821)
                     /locus_tag="JN701_004116"
     CDS             complement(495..821)
                     /locus_tag="JN701_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366359.1"
                     /translation="MFKYAEMLMAEEDNKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALGVNSWRDKSKYLDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          650 a          353 c          325 g          566 t
ORIGIN      
        1 ctccatacag gataattaga ggtcagtttg attattcaca attcattatc agcattttct
       61 gtttctgacg aaatcaacat gaaaagaacc atatatgata attattataa taacggcttt
      121 aattggaata catatattac aacgtattat atataattgg tattctggga actatattct
      181 caaaatacag tagaaacgag gtatgtttct ggtggaaagg acagtgggat taaaaagtaa
      241 gaattgataa aaaaacgcca gcaacacata gtgccggcgg agggaatacc ccatggagaa
      301 aatgtgatgc ctagagcacc atgaatatat aattaaaaat agttagagtt gtcaatactt
      361 tcacaaaaat aattttcgta gtttcgtagt atagaaagat atttttatgc atgacctacc
      421 tgaatttgct ccgggtagag gttataaata aaaattgaat cacgacaaac acaatattca
      481 tagtatggcg atgcctacgc cagcaagaat agcgccaata atgttgggaa tataatagac
      541 accagacgca caggcatctc atctcttaac aaataataat caaggtattt acttttgtct
      601 cgccagctgt ttacacctaa agcacacaag attaaccatt caataacaaa tggcaatatc
      661 catagccata cgactttacc ttgtaatgtt cggtatttct ttataattat tctgggaaat
      721 ctatcattta tttttaaaat caaattatct tgttgtttaa aaataagttc acatacttta
      781 ttatcttctt ccgccatcaa catctctgca tacttaaaca tttcagaacg ttcctttagc
      841 acagaagagt aattatatgt ccagttccca ggcaataatg cttatggata tttaactaat
      901 aatttagaga atattttgca ataatatttg gctgtattca gaaatacctg aaaaatcata
      961 ctatacagcc ccagggaatg gataaagatt ctaacaaagc atttcaacaa tatatgcttg
     1021 ttaaaaatcc atatcgaaat gccgttgcag cattaatata tgcctcattc ataaaatgta
     1081 aaagagcaag ctcgtaccag tagggggaaa ttcattgaca tgtcctgtca atacgtacag
     1141 agccctgtca tgcttgccag cacgtaacaa ttccgtcccc agtaaccagc gtgtcccctg
     1201 attattctca gggttatatg ttagtatttt atcaatgagt ataacggcat cctgatgacg
     1261 ctgtaaatga acattcgcca gaattgcagc ataaagagcg caaatgcata tgccagatga
     1321 gcgtgaatat caatgatgtc aggagcatgt caggcaagac gcctgagttt gttgacgtaa
     1381 tttgtttcgt ttatttcatc actatcgtat gcgtcaagag ttttttcaaa ctcatcccga
     1441 aatgggccaa gcctggtttc aggaaataag aaatacccct agttattgtt cacttaaaag
     1501 tataatttgt aattcaaatc ctgagcctgt aaaacgggga gtaacatatt ctattctgaa
     1561 gagaataaaa gtcgtgatgc gatgtatcaa gcccggattg taatcccaga ttaacataga
     1621 tcacaaaaca acttattctt cactaacgtg aagataaata tcgttgtatg ccttatcacg
     1681 actacgaata ccagcaagat acaactgaca tacggaaaga taccatgttt tttactcccg
     1741 aaaataacgc taaaaagcta cttccccatc gtttgtcctt agtattgtca gcgccaacaa
     1801 tgtgggctga catgataaag ctgtctagga aaacgttcgc ctcctcagcg gacaatccaa
     1861 atggtgattg tccctattaa acgtttattt tgaa
//
LOCUS       DADLTD010000339         1893 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.343, whole genome shotgun sequence.
ACCESSION   DADLTD010000339 DADLTD010000000
VERSION     DADLTD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.343"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..815)
                     /locus_tag="JN701_004117"
     CDS             complement(<1..815)
                     /locus_tag="JN701_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7366360.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JN701_004118"
                     /pseudo
     CDS             907..993
                     /locus_tag="JN701_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1003..1083)
                     /locus_tag="JN701_004119"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="JN701_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1294..1383
                     /locus_tag="JN701_004120"
                     /pseudo
     CDS             1294..1383
                     /locus_tag="JN701_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1407..>1893
                     /locus_tag="JN701_004121"
     CDS             1407..>1893
                     /locus_tag="JN701_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7366361.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQ"
BASE COUNT          501 a          371 c          486 g          535 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gtaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgctcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtaatctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcaacta acccagtgcg ttgacaaaaa attagcgcaa gaagacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac cagctaagta gttgattcat agtgactgca
     1201 tatgttgtgc tttgtcgcat tttgtagtct attatttaat ctgtaacaac gataatacaa
     1261 tgaattttaa ttggtttttg tgattttgtc cttcttctga catggaagga cacccgatgg
     1321 atacccaacg attccaatcc caatttcatt ggcatttatc gtttaaattt tctggcgcga
     1381 tagcagcgtg cttgtctctg tctcttgtgg gaacagggct tgctaatgct gatgattcgc
     1441 ttccttccag taactatgcg ccgcccgccg ggggaacatt ctttttgctt gctgacagca
     1501 gttttagcag cagtgaagag gcgaaagtgc gactggaagc gccggggcgt gattatcggc
     1561 gctatcagat ggaagagtac ggcggcgtgg acgttcgcct gtatcgtatt cctgacccga
     1621 tggcattttt gcgccagcag aaaaacctgc atcgcattgt ggtgcaaccg caatatctgg
     1681 gcgacgggct gaacaatacg ctgacctggc tgtgggataa ctggtacggc aaatctcgcc
     1741 gcgtaatgca gcgtactttc tcttctcagt cacggcagaa tgtgactcag gcattacccg
     1801 aattacatct cggcaatgcc attattaaac cttcccgtta tgtacagaac aaccagtttt
     1861 ccccgctgaa aaaatatccc ttggtggaac agt
//
LOCUS       DADLTD010000340         1891 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLTD010000340 DADLTD010000000
VERSION     DADLTD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.344"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            95..277
                     /gene="tnpB"
                     /locus_tag="JN701_004122"
                     /pseudo
     CDS             95..277
                     /gene="tnpB"
                     /locus_tag="JN701_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            297..>1891
                     /locus_tag="JN701_004123"
     CDS             297..>1891
                     /locus_tag="JN701_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HAY7366362.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVL
                     APGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADA
                     YAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGS
                     PAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDG
                     WVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLRE
                     VIEKLNDWPSNQVHELLPWNFSSV"
BASE COUNT          523 a          458 c          537 g          373 t
ORIGIN      
        1 ggatgcgttc atataaaaac aatggatgcc ttgcaaacgg gatgcgttca tataaaaaca
       61 atggatgcct tgcaaacggg atgcgttcat ataaatactg tgggccgatc gtgacgggtt
      121 atgcctgttc gccaaacgcc tggaacgggg ccgcttcgtc tggccggtga cccgggaagg
      181 gaaagtgcac ctgacgccag ctcagttatc catgctactg gaggggatcg cgtggcaaca
      241 tcccaaacgg acagaacggc ctggcatccg gatataaccc gtgataaaac aagggaatga
      301 acaatgaact ccccgatgat attgagctgc ttaaagccat gttgcgtaag caacagagtc
      361 ggcttcgaca gtatgcctgt caggtcgcgg gctatgagca ggaaattgaa cggctgaaag
      421 cgcaactcga caggttgcgt cgtatgttgt tcggccagag ttcagagaaa aagcgtcata
      481 agcttgaaaa tcagatccga caggcagaaa aacgactgtc ggaactggaa aaccgactga
      541 acacagccag aaatcttctg gaagatgcat cgtcagtcac agattcacct gacaccagtc
      601 ccccgtcaga aaacccgatc gccagtaagc ctgaatcccc gggacgaaaa tcttcacgaa
      661 aaccgctgcc ggcagaactt ccccgggaga cacatcgcct tctgcctgct gaaaccagtt
      721 gcccggcctg tggaggtgtt ctgaaagaaa tgggggaaac aatctcagag caactggata
      781 tcattaatac cgcctttaaa gttatcgaaa ccatacgtcc caaactggcc tgtagccggt
      841 gtgatgtcat cgttcaggca ccacttcccc ctaaaccgat cgaacgcggt tatgccagtg
      901 cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct ttatatcgcc
      961 agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg gtgcgctggg
     1021 tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac tatgttctgg
     1081 aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg gggaacggaa
     1141 agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg ggttcatccc
     1201 tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat ccgcagctcc
     1261 acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat aacgtactgt
     1321 acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga aaaatccatg
     1381 acgaggatgt gcgccgtccg acagaaatga ctcaggaagc gctcagacgg atagcagagt
     1441 tatacgacat agaagcggag atacgtggca gtccggcaga ggaacggctt gcagtcagaa
     1501 aagccagaag cgtccagttg atgcagtcat tgtacgactg gatacagttg cagaggaaaa
     1561 cgctgtcgaa acatgcggag atggcgaagg cgttcgacta tatcctgaat cactggaatg
     1621 cgctgaacga gttctgtcgt gacggctggg tggaaataga caacaacatc ggtgaaaacg
     1681 cgttacgatc ggtggcggtt ggaagaaaaa attatctctt tttcggctca gacaagggag
     1741 gagaaagtgc ggcgatcatc tacagtctgc tggtcacctg caaacagaac gaagtggagc
     1801 cggaggactg gttgcgcgaa gtgatcgaga agctcaatga ctggccgtcg aaccaagtgc
     1861 atgaactgct gccctggaac ttctcgtctg t
//
LOCUS       DADLTD010000341         1886 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLTD010000341 DADLTD010000000
VERSION     DADLTD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.345"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>1886
                     /locus_tag="JN701_004124"
     CDS             <1..>1886
                     /locus_tag="JN701_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7366363.1"
                     /translation="FTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATY
                     FGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSA
                     KHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAP
                     TYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANG
                     AISAASSDAINGSQLYTTNKYIADALDGDAEVNADGTITAPTYTIANAEYNNVGDALD
                     ALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMM
                     IEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVA
                     KGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELL
                     GALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLA
                     VGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTT
                     TRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDER
                     VAQNT"
BASE COUNT          497 a          478 c          489 g          422 t
ORIGIN      
        1 ttcactgcag ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca
       61 gtcactgcaa ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat
      121 atcgccacct acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca
      181 acctacaaaa tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac
      241 tcctcattta gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc
      301 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
      361 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
      421 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
      481 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
      541 accactcttg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
      601 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
      661 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
      721 gctgatgcgc tggatggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
      781 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ctctggatgc gcttgatgat
      841 aacgccctgc tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat
      901 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
      961 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     1021 attatcaatc agctcgctgg taacaccgac gcaacctata tccaagaaaa cggtgcgggt
     1081 attaactatg tgcgtactaa cgacgacggc ttagcgttca acgacgccag cgcacagggt
     1141 gttggcgcta cagctatagg ttataactct gtcgccaaag gcgatagcag cgtagctatt
     1201 ggtcagggca gctacagcga cgttgatacg ggtatcgccc taggtagcag ctctgtttcc
     1261 agccgagtga ttgccaaagg ctcccgtgac accagcataa cggaaaatgg cgttgttatt
     1321 ggttacgaca ccacggatgg cgaactgctc ggtgcattgt ctatcggtga tgacggtaaa
     1381 tatcgtcaaa tcatcaacgt agccgatggt tccgaagccc atgacgccgt tacggttcgt
     1441 caattgcaga atgcgattgg tgcggtcgca accacgccga ctaaatactt ccacgctaat
     1501 tcaacggaag aagattcact ggcagtggga actgactcgc tggcaatggg tgcgaaaacc
     1561 atcgtgaatg gcgataaagg tattggtatc ggttatggtg cctacgtgga cgcgaatgca
     1621 cttaacggca ttgccattgg tagcaatgcg caagtcattc atgtcaacag tattgcgata
     1681 ggtaatggtt ctacgaccac tcgtggcgct caaaccaatt ataccgccta caacatggac
     1741 gcaccgcaga actctgtcgg tgaattctca gtcggtagtg cggatggtca acgtcagatc
     1801 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     1861 actgatgagc gcgtagcgca aaatac
//
LOCUS       DADLTD010000342         1862 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLTD010000342 DADLTD010000000
VERSION     DADLTD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.346"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..844)
                     /locus_tag="JN701_004125"
     CDS             complement(<1..844)
                     /locus_tag="JN701_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7366364.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSAVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
     gene            complement(845..931)
                     /locus_tag="JN701_004126"
                     /pseudo
     CDS             complement(845..931)
                     /locus_tag="JN701_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(1025..>1862)
                     /locus_tag="JN701_004127"
     CDS             complement(1025..>1862)
                     /locus_tag="JN701_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7366365.1"
                     /translation="LSSYNADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFII
                     MRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIY
                     NIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINA
                     QWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIADRIRKN
                     VELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIH
                     HI"
BASE COUNT          546 a          421 c          418 g          477 t
ORIGIN      
        1 gaagtggcac actgaatttg gccacttcac gccccgaatc ggtatcggtt agcgtgacat
       61 tgagaaaacg ccagaggaga gggcggtcat gagtgtaaaa gatattccgc aaattgtttt
      121 tgttaatatc cagcagcaca atccctttta gctgccctgc aacatacacc ggcgtcagca
      181 aactcatcac gttctgttta gtacgctgat cctggtaaat gctcgacaat acggtacgcc
      241 cggtaaatac cttttcaata tctttgcgat cgataccaat acttcctgac tgcaggaagg
      301 accagttatt catcgcaaag tattcaacat taaccggctc ataaaaatag acatagcggt
      361 cattgagatc gagataatag tgaaatttct cttcggccat cccaagacct gaaccgtaat
      421 catcctggtg gcggttgcct tccactgcct gatcaaaagc gggtaatagt gctgcagctt
      481 ccacaattgt gtcgcagtca gtggatgcgg tttgtagtgt gccgcgtaag cccgggtatt
      541 tatgagcagt caggtttaag ccatgggtat tattggcaga ctcaaaagcc tcgcagatgg
      601 cgcgccgctg ttctggcgac actgccgact gctcagcagc gagagcgcgc atcacatgcg
      661 cggcaatact ctgattctga tacttatcat aaagaaagga ggagtctgct ttctgcacga
      721 tatagcgcag ataggcactc attgctctgt cgctggcgac aagttcaaaa ataaggaagg
      781 aagatgtcag cacaatgaca cttgcggcga taaaatgccg gagcattcta tggtgcaatt
      841 tcatgataaa tggcactcct tcagttagtg aaacgagaaa accagataaa gttcgattgc
      901 ggcctggcac aggggcgcta attatactca tctctgcgcc gctggggagg ggaaatttag
      961 gggtaatagt aataaattgt atgcaaaagc cacgcaaaaa gcatggctct gaatattttt
     1021 gcacttaaat atgatgaata atcactttgt tgcccccctc gcttttcgct tcgcgtaagg
     1081 ctttatctgc ttccgtcatg acaagcgaga tattacgcgt gtcacccgtt gaaaaaaccg
     1141 tgccaatact gatggtcatt tgttcaggaa catcatatct gttactaaag ccggtcagga
     1201 gttcgacatt tttacgtata cgatccgcaa tgatttttgc ctgggcacta ttgagttcgg
     1261 taaatagcaa gccaaacacc tcaccctcca gtcgcgctaa aatatcatca gggcggatgc
     1321 tttgctggat aatatcgaca attgaaacca gcactttatc acccacacga tgtccccatt
     1381 gggcattgat gcgtttgaac tgattaatat caaccagcat gacacagaag tccttaacaa
     1441 caggtcgcga caataatgac tctacgctgt tgaaaaagta gcgccgatta taaatattgg
     1501 tcagcacatc atgaactgcc agtttgctgg agagttgcag ctcctgaaaa atgttataaa
     1561 tcagaaaaga cacaacaaac agtttactgg aaacttcaat cccgcgactg atataccaga
     1621 tggtgtaatt atactcatcc agagtcagta gaagtaagtt gcagcatagc gatgccagac
     1681 ataataatgc tattaacggc catagttcgc tggctaatcg tgtacgcatg ataataaaga
     1741 acagtaaaaa tgcccacaga caaaccaata tttttgtata gttgattccc ctggtcgcag
     1801 tatggttgtc cggacagtag tcgacgacat acaaagaata gtcagcgtta taactgctca
     1861 ga
//
LOCUS       DADLTD010000343         1852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLTD010000343 DADLTD010000000
VERSION     DADLTD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.347"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(549..625)
                     /locus_tag="JN701_004128"
     tRNA            complement(549..625)
                     /locus_tag="JN701_004128"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(589..591),aa:Arg,seq:tct)
     gene            complement(639..715)
                     /locus_tag="JN701_004129"
     tRNA            complement(639..715)
                     /locus_tag="JN701_004129"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(679..681),aa:Arg,seq:tcg)
     gene            complement(723..798)
                     /locus_tag="JN701_004130"
     tRNA            complement(723..798)
                     /locus_tag="JN701_004130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:Ile,seq:cat)
     gene            complement(839..1804)
                     /locus_tag="JN701_004131"
                     /pseudo
     CDS             complement(839..1804)
                     /locus_tag="JN701_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403604.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          438 a          533 c          448 g          433 t
ORIGIN      
        1 ttttccgcaa gatctgacgg cgacgccgcc ctgacaacat catagtgttt aaaagccatg
       61 aatcctcccg gccgggataa tattgtgagt aaaatgagga gcgggctgaa gtccggaagt
      121 tacaggacaa tggcagaaga gagacgacag cccgcaattc gaaaaagacc gcgcagttgc
      181 gcagagtgac tactatgggg tattattcgc cagctgaaat attacttcac gttttattgt
      241 ttattccttg ccgcccgcgt ctcccagcgc gggctttttt tgcccacaag aaagcccctc
      301 cggagagggg ctggagagtg gcgctatgtg ccattgcatg gtgccgggtg cctcccggtg
      361 aattcagtac cagcacctga atccgcgatt atcccatata cctactcgct gattgcccct
      421 ccgcacaggg ggattcacca tgccagtttc ttttaacaaa ctccccgcaa accagacaac
      481 agtcaaccgt ctgaattgtg agacatttaa aaaaaaagcc cgcaaaagcg agccagggaa
      541 aataagtgtg gcgcgttgta ctggattcga accagtgacc gattgcttag aaggcaattg
      601 ctctgtccgg ctgagctaac aacgcatgat gcagataatg gaccgccatc ggggacttga
      661 accccgcgca gccagcttcg aaggctggcg ctctttcctg atgagctaat ggcggtatgt
      721 gatggtggcc cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca
      781 ccactgagct aaagggccgg gagccgcata ataacgacgc gtaattaatt cttcaatatc
      841 atccgttctg gctgactaaa tcctgtactt cccgaaccgt ctgctcaaaa cgttcagtct
      901 ccagctcaac gccaattgca cgacgcccga gcgccagtgc cgctttcact gtcgaacccg
      961 accccatgaa aaaatctgca atcaggtcac ccggacgact gctagcgctg attatctgct
     1021 gcagcatttc tgccggtttt tcgcacggat gtttcccggg atagaactgc accggtttat
     1081 gcgtccacac atcggtatac ggcacctgca ccgtcacgcc aaaataccgc cgcagatgtt
     1141 tatattcact cagcagctcc gcatactgcc ggttcagtga ggtatacgta tccaccagct
     1201 ggtggtggag cttttccagt tcaccacgcc gatgcttctc ttctgccacc cgggcaaaca
     1261 gcgcctgtaa tttcagataa tcgctttcat tcggcaactg ccactgactg gcactgaacc
     1321 agtgcgacac catgtttttc tttcctgtgg catctgcaat ctgttttgcc gttatcccca
     1381 gggcagctcg cgcatcacga aagtaagaaa tcagcggggc catcacatgc tgtttcagtg
     1441 cactgccctt cgcctcatag ccatcatctt tcgggcggta cggcccctga taatgttccg
     1501 cgaacagaat gcgctctgtg gcggggaaat acgcccgcag gctttccttg ttgcatccgt
     1561 tccagcgtcc ggacggcttc gctcagataa tatggttcag cacactgaag cgttcacgca
     1621 tcatgatttc gatatcagat gccaggcgat gaccacagaa caggtaaaga cttccggcag
     1681 gtttcagcac ccgccagaac tgcgccaaac actgctccag ccacttcagg taatcatcgt
     1741 cgcccttcca ctggttatcc cagccctcag gcttcacttt aaagtacggc gggtccgtga
     1801 ctatcaggtc aacagaattt tcgggtaacg accagataaa ttccaggcag tc
//
LOCUS       DADLTD010000344         1816 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLTD010000344 DADLTD010000000
VERSION     DADLTD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.348"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(317..1465)
                     /locus_tag="JN701_004132"
                     /pseudo
     CDS             complement(317..1465)
                     /locus_tag="JN701_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          575 a          464 c          394 g          383 t
ORIGIN      
        1 tggttggcgt agttattaac aacgttgcca aaaccccaga cagtgataaa agacatcagc
       61 gcaatgttgt accatcgcag ctgtttttcc tgaacattcc cagccataga caaatccttc
      121 gccaaaatta taggggcatc tgtaaaacga gggattatgc gcggcaaata agaagtgcgg
      181 aaatagcgaa atgcaaaatt ataacctgct gcacatttta tttttaaacg aataaaattt
      241 ggaataaagc aagtcagata gaagaaacaa aaaagcagag tgtgaaacag taagttcaca
      301 ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg gcgttgcgca
      361 gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat aaattatcgg
      421 agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc acagtatagc
      481 tcaacagtaa ggcctggttc atcgccacca gttgatgatg ttcaactttc tcgcaagccc
      541 atgccatcgc caccggatat agcgtaaagc cagcggcacc gaggatgaat aacgctgggg
      601 ccatcgccgc ctagctaagc atcgcgatac tgccgagaat gacgacaaag acctgaacac
      661 gcaacaccag cagtcgacca aacttatccg ccagacgtcc aatcgaccat tgtccaagga
      721 tacccgcact gaccagtacc gccatccaga aaccaatgct ggcattgctc acccctttgt
      781 gattgaggta cagcggcatc aggccatata gagatcccag aacgattcct gagataatgc
      841 agccattcac gccaagccgc gcctgacgga gttttagcat tgacgtaatt gacgtcgaat
      901 catggttttc agcctgctga ttcagcacac gcgtaaacaa cagcggtaag atccctgcca
      961 acgtcaaacc tgtaacccac ggcaatacgg acatcagctc ggttgaaact ttgctgacca
     1021 gtaactggcc taaaaacgtt cccacgtaat aaaccatcat atacgcagca agcaaacgcc
     1081 cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacaaccacc caaatcatgg
     1141 cacagccgac gcccgcgaca aaacgccaag ccaaccagct ccagaatcca atcatcaggc
     1201 caaggccggc acagccagcg gcaaaaatga aggaggccag ataatagctg cggttaaagc
     1261 caatgcgctt aatgacatac cctgtcagca atgtaccgac aaggttgccg gtaaaatagg
     1321 atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc caaagcggca
     1381 cgagtgtatt taacaccgca atcgccagag tcaacaaaag caggccagac agcaaaagca
     1441 tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa atttcatgcg
     1501 catcatgcca ccgccaaaac attgtcaatg agtgaaaata cgcgatatcc cagcggcggt
     1561 attatcgatt tatattactc attcataacg ctaatgctga agagatgatt agtctcatct
     1621 gattgttttt attgaactaa cacaaatttc atacgcttcg ttcagtcgaa aaatttcatg
     1681 gtcacagcaa actcatattt cccgtctatg ctttcagagc aatgcatcgc gtcggatcga
     1741 tttcagacgt accactgact ctcgaaagga gaagaggatg accaaaccgt atgttcgtct
     1801 tgataaaaat gatact
//
LOCUS       DADLTD010000345         1761 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLTD010000345 DADLTD010000000
VERSION     DADLTD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.349"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..276
                     /locus_tag="JN701_004133"
     CDS             <1..276
                     /locus_tag="JN701_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309007.1"
                     /note="oxidoreductase, Fe-S subunit; terminal electron
                     transfer protein for the reduction of DMSO; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY7366366.1"
                     /translation="QYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDEL
                     RKKHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            278..1141
                     /locus_tag="JN701_004134"
     CDS             278..1141
                     /locus_tag="JN701_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7366367.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(1176..1757)
                     /locus_tag="JN701_004135"
     CDS             complement(1176..1757)
                     /locus_tag="JN701_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005135793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7366368.1"
                     /translation="MLVDHQAGLLSLVRDIEPDKFKNNVLALGDQAKYFNLPTILTTS
                     FETGPNGPLVPELKAQFPDAPYIAHPGNINAWDNEDFVKAVKATGKKQLIIAGVVTEV
                     CVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGAQLMTWFGVACELHRD
                     WHNDIEGLATLFSNHIPDYRNLMTCYDTLTKQK"
BASE COUNT          379 a          423 c          489 g          470 t
ORIGIN      
        1 caatataacg aaacgaaagg ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg
       61 gagggtaaaa agccgatctg tgttgaatct tgtccgctgc gggcgctgga tttcggccct
      121 atcgacgagc tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt gccgcgagct
      181 cactttacca aaccgaatat tgtgatcaaa cccaatgcca atagccgccc gaccggggat
      241 accactggct atctggcaaa cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg
      301 gccgctgatg atcttcacgg tcttcgggca atgtgtagca ggtggtttta tcgttctggc
      361 tttggcgctg ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta tcgcctgcat
      421 gtttggttta tgggtgctga tgggcattgg ctttatcgcc tctatgctcc atcttggttc
      481 accaatgcgc gcttttaact cgctcaaccg ggtaggggct tcagcactca gtaacgaaat
      541 cgccagcggt tcgatctttt ttgccgtagg cggcatcggc tggttgctgg caatgctgaa
      601 aaagctgtca ccggcattgc gtacgctgtg gctgatagtg acgatggttc ttggcgtcat
      661 ctttgtctgg atgatggtgc gtgtgtataa cagcattgat accgttccga cctggtacag
      721 catctggacg ccgatgggct tcttcctgac gatgtttatg ggcggcccgt tactgggtta
      781 cctgttgttg agtctggctg gcgtcgatgg ctgggcgatg cgtctgctgc cagcaatttc
      841 tgtactggca ctggtagtga gtggcgtggt gtcagtgatg cagggcgcag agctggcgac
      901 tattcatagt tctgtgcagc aggccgcagc gctggtgccg gactatggtg cgctgatgtc
      961 ctggcggatc gtgcttttgg ccgttgccct gtgcttgtgg attgcaccac agctaaaagg
     1021 ttatcagcct gcggttccgc tactttctgt atcattcatt ctgcttctgg caggggaatt
     1081 aatcggtcgc ggcgtattct atggtttgca tatgaccgtg gggatggccg tcgcaagcta
     1141 atcataacaa ccggggtttc ggccccggtt ctcttttatt tctgcttcgt taacgtgtca
     1201 taacaggtca tcaaattacg ataatcagga atgtggttag agaacaacgt cgccagtcct
     1261 tcaatatcat tatgccagtc gcgatgcagc tcgcacgcca cgccgaacca tgtcatcaat
     1321 tgcgcaccag cttgcgacat gcgatcccat gccgaatgcc gggtaatttc attaaaggtc
     1381 cctgacgcgt cagtcacgac aaagacgtca aacccttctt caatggctga aagcgccggg
     1441 aatgccacgc aaacttcggt taccacaccg gcaataatta actgtttttt acctgtcgct
     1501 ttgacagctt ttacaaaatc ttcgttatcc caggcgttaa tatttcccgg gtgagcaatg
     1561 taaggtgcat cggggaattg tgctttcagt tctggaacta atgggccgtt aggaccggtt
     1621 tcaaaactgg tggtaagaat agttggtaag ttgaaatact tagcctggtc acccagcgcc
     1681 agcacgttat ttttaaactt atcgggttcg atatcccgta caagggaaag taaaccagcc
     1741 tggtgatcaa caagcaaaac g
//
LOCUS       DADLTD010000346         1757 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLTD010000346 DADLTD010000000
VERSION     DADLTD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.350"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..73)
                     /gene="csgC"
                     /locus_tag="JN701_004136"
     CDS             complement(<1..73)
                     /gene="csgC"
                     /locus_tag="JN701_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY7366369.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDV"
     gene            complement(132..587)
                     /locus_tag="JN701_004137"
     CDS             complement(132..587)
                     /locus_tag="JN701_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major curlin subunit CsgA"
                     /protein_id="HAY7366370.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(628..816)
                     /gene="csgB"
                     /locus_tag="JN701_004138"
                     /pseudo
     CDS             complement(628..816)
                     /gene="csgB"
                     /locus_tag="JN701_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
     gene            1513..>1757
                     /locus_tag="JN701_004139"
     CDS             1513..>1757
                     /locus_tag="JN701_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY7366371.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNN"
BASE COUNT          506 a          373 c          333 g          545 t
ORIGIN      
        1 acacatcccc ttgctgggtc gtattaaagg ttatctgact ggaaagtgcc gcaaggagta
       61 ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctgtaata
      121 caaatgatgt attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg
      181 ttgacggagg agttagatgc agtctggtca actgcagcac cgttgccacc accgaactgt
      241 ttaaccgtca tttcagaatt tttgccgttc cactgatcaa gagtagcgct attaccgaag
      301 ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc tgcaccatta
      361 ccgccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg cagagcaagt
      421 gcagagttac cgccaccgta ctggtaaatg ttcagctcag aatttgggcc gctattatta
      481 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
      541 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
      601 cggattgatt taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga
      661 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
      721 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
      781 atcgttggca ctgcccgcct gatcaatata tgccagtcta cgcaacctgt attttgttaa
      841 cgctgcgtta tgatggaaag tatgtctgcg gaaatatttt taataactca cccgcgcgtt
      901 gtatttttct ttttttgttc ataccaaagt attaaaaccc acgtaaatac gctgttatct
      961 acgcaaaaaa tattttgttt tcttttaaat ctccgttttc cgctatcaaa aatcaccaga
     1021 cagtcatttt tcttgcccgt cgctgattgc tgcgatatgt cttgcgcaca agccgtgaca
     1081 aacctcgtct ttttggagta aaaatttgtc cacggaggtg cggagaaaaa acaagaacgt
     1141 tttacatgac gaaaggacta caccgaaata ttttttatat gtattattag taagttatca
     1201 ccatttgtat gattttttaa aattgtgcaa taaaaaccaa atgtacaact tttctatcat
     1261 ttctaaactt aataaaacct taaggttaat attttaatat aacaagttac atttagttac
     1321 atatttaaca cttgatttaa gttttgtaat ggctagattg aaatcagatg taatccatta
     1381 gttttatatt ttacccattt agggctgatt tattactaca cacagcagtg caacatctgt
     1441 cagtacttct ggtgcttcta ttttagaggc agctgtcagg tgtgcgatca ataaaaaaag
     1501 cggggtttca tcatgtttaa tgaagtccat agtattcatg gtcatacatt attgttgatc
     1561 actaaacctt ctttgcaggc gacagctctc ttgcagcacc ttaaacaatc gctggcaatt
     1621 acaggaaaat tacataatat tcaacgttct ctggacgata tctcttcagg ctctattatt
     1681 cttctggata tgatggaagc agataaaaag cttatccatt attggcagga tactttgagc
     1741 aggaaaaaca acaatat
//
LOCUS       DADLTD010000347         1736 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLTD010000347 DADLTD010000000
VERSION     DADLTD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.351"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            67..807
                     /locus_tag="JN701_004140"
     CDS             67..807
                     /locus_tag="JN701_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7366372.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            842..1736
                     /locus_tag="JN701_004141"
                     /pseudo
     CDS             842..1736
                     /locus_tag="JN701_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          502 a          368 c          369 g          497 t
ORIGIN      
        1 aatagtgtta ataatttcta tttttatatt attccctgtt ttaattaact ctgtcaggga
       61 tggtttatgt tttttaacac caaacataca acagctttat gcttcgtaac ctgtatggct
      121 tttagttcat cgtctattgc ggacattgtc atttcgggta ctcgcgtaat atataaaagc
      181 gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga ataacccgtt gcttgtccag
      241 agttggttag atactggcga tgacaacgct gagcctggca gtattacagt cccttttact
      301 gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc aaacaatcaa attaatgtac
      361 acagccagca cctcactgcc taaagacaga gagagcgtgt tctggtttaa cgtactggaa
      421 gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa gtctgctgca actggcattt
      481 cgcacacgta taaaactttt ctatcgcccg gatggattga agggaaatcc ctctgaagcc
      541 ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta aggcgtcatt gcgagtgacc
      601 aatccaaccc cttactacgt ctcttttagc agtggtgatt tagaagctag cggtaaacgc
      661 tatccgattg atgtgaaaat gattgcacca tttagtgatg aggtcatgaa agtcactggc
      721 cttaatggca aagcaagttc tgcaaaagta catttttacg ccattaatga ttttggtggc
      781 accattgaag gtaatgccag tctgtaatca ggcaaggata taattccgca ggaagcataa
      841 cgtgactata aaaccaacta aaaattatca taatcatctg acccgtatcg ctacgttttg
      901 cgcgctgctg tattgcaata gtgctttctg tgctgaactt gttgaatatg accatacctt
      961 cctgatgggg cagaatgcct caaatattga tctcagccgg tatagtgaag gtaaccctgc
     1021 tataccgggt atgtacgacg tcagtgttta tgtcaacgat caacctatta ttaaccaaag
     1081 cattacattt attgaaattg aaggaaaaaa aacgcacaag cctgtatcac acttaagaat
     1141 ttattgcagt ttcatataaa ttctcccgat ataaataacg aaaaagccgt tctgcttgcc
     1201 agggatgaaa cactcggcaa ttgccttaat ttgacggaaa ttatccctca ggcttctgtt
     1261 cgttatgacg ttaacgagca acgtctggat atagacgttc ctcaggcatg ggtaatgaaa
     1321 aattaccaaa actatgttga tccatcgtta tgggaaaatg gcattaatgc ggccatgttg
     1381 tcatacaacc tcaacggata tcatagtgaa actcctggtc gaagaaatga cagcatttat
     1441 gctgctttta acggtgggat gaatttaggt gcatggcgac tgcgtgcctc aggcaactac
     1501 aactggatga ccgattctgg cagtaattat gattttaaga atcggtatat acagcgtgat
     1561 atcgcctcgc tgcgttctca actcattctt ggtgagtctt atacaacggg cgaaaccttt
     1621 gattctgtca gtatccgcgg cattcgttta tacagcgaca gccgcatgtt gcctccgaca
     1681 ttagcgagtt ttgcgcctat cattcatggc gttgctaata ccaacgccaa agtcac
//
LOCUS       DADLTD010000348         1715 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLTD010000348 DADLTD010000000
VERSION     DADLTD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.352"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            122..1399
                     /locus_tag="JN701_004142"
     CDS             122..1399
                     /locus_tag="JN701_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7366373.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
BASE COUNT          447 a          368 c          372 g          528 t
ORIGIN      
        1 ttttaaaggg agaaatttac acacaataat tttcaatatt tcctctgtac ctgattttta
       61 ttacctatta cacggaaata ccaacaacca gggtttatta atataattaa aaggtttaaa
      121 catgaataaa ataaagaatt accggtggca tatgattgcc ctcgtatgct ttatcactgt
      181 aatcaattat ctggacagaa cggcgttagg tattgcggct ccaacgatta tggagacaac
      241 cggaataact aaagagcaat attcatggat tgtcagtgca ttccagttgg cctatacatt
      301 agggcaaccg gtaatgggct tctttattga taccgtgggt ctgaagttaa gttttgcgat
      361 atgtgccgca atttggggcc tggcgacaat gggccatgca ctaaccggaa cgtggtctgg
      421 tctggcattt atgcgcgccc tgatgggttt cagcgaagcg tcggcaattc cggcgggtgt
      481 aaaaaccgca tcaacatggt tcccggcaaa agagcgtggc gtggcgacag gtgttttcaa
      541 tatgggcacc tcactcggcg cgatgcttgc tccaccgttg attgcctggt gcattatgtt
      601 tcatagctgg caatttgcgt ttattgtttc aggtagtctt gctttgctcg cggctttatt
      661 ttggttcttt tgttataaag atccgaaaga tgccaaacgc ctttctgatg aagagcgcca
      721 ctatattgaa tcagggcaag aacagcatct taaaacagat aagaaagaaa aaacgtcaat
      781 caagcatatc ctcagccaac gtaatttctg ggggattggc atcgcgcgtt ttctcgcaga
      841 cccggcatgg ggaaccatta acttctgggt gccgattttc ttcgtcgaaa cgctgcattt
      901 tagcctgaaa gaaattgcca tgttcgtctg gctgcctttc ctgctgggcg atctcggctg
      961 tttagccagt ggttttgtcg cgaagttctt ccacgatcgc ggcgtgagtt taattaactc
     1021 acgaagaatt accttcacta ttgcagccgt cattatgatg acgattggcc tggtgagtat
     1081 tgtcgaaaat ccctacattg ccgtattact gattagtatt ggcgcgttct cgcatcaatg
     1141 tctttctact gtagcagcaa ctctgggtgg cgatctgttc aaaaaagacg aagtagctac
     1201 cgcagtgggt atggcaggag cctgtgcgtg gagcggtcag ttgattttca acctgttcat
     1261 cggggcattc gttcacatta tcggcttcgc gccgttcttt attgccctgg ctttctttga
     1321 cattattggt gctattgcgt tgtggacgct tatcaaagtt aaagatgaag aaccacaagt
     1381 acagttagct acccactaat tattttaaat aaaaacgcta taattgaggt tatggcgttt
     1441 ttaaagaagc ttgagttatc gaaccccgct taccaacgcg ttgtcagtca tctcccccct
     1501 ccctggtgca tcgttatatc cgtgatgagc gatttttgat attaatatct ttcaccatcc
     1561 gctattttca ccacttcaga aaacaaactt atttttatta atatcaattc tttttctttg
     1621 aacattcaac tgcgatattt gtaaaaacat taaacaggca acaataaaat gacattacca
     1681 acaactattt attcattccc cgcatattta cctcg
//
LOCUS       DADLTD010000349         1713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLTD010000349 DADLTD010000000
VERSION     DADLTD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.353"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1415
                     /locus_tag="JN701_004143"
     CDS             <1..1415
                     /locus_tag="JN701_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7366374.1"
                     /translation="QNLTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDR
                     AAILFKIADRMEQNLELLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGG
                     ISEVDSETVAYHFHEPLGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLS
                     VLLLMEIVGDLLPPGVVNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQ
                     NIIPVTLELGGKSPNIFFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQES
                     IYERFMERAIRRVESIRSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGG
                     RRKLLEGELKDGYYLEPTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQY
                     GLGAGVWSRNGNLAYKMGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLE
                     HYQQTKCLLVSYSDKPLGLF"
BASE COUNT          407 a          417 c          509 g          380 t
ORIGIN      
        1 accagaacct gacgccggtg actgggcagc tgctgtgcga agtggcgtct tcgggcaaac
       61 gagacatcga tctggcgcta gatgctgcgc acaaagtgaa agataaatgg gcgcacacct
      121 cggtgcagga tcgcgcggcg attctgttta agattgccga tcgaatggaa caaaacctcg
      181 agctgttagc gacagctgaa acctgggata acggcaaacc cattcgcgaa accagtgctg
      241 ccgacgtgcc gctggcgatt gatcattttc gctatttcgc ctcgtgtatt cgggcgcagg
      301 aaggtgggat cagtgaagtt gatagcgaaa ccgtggccta tcatttccat gaaccgttag
      361 gcgtggtggg gcagattatc ccgtggaact tcccgctgct gatggcgagc tggaaaatgg
      421 ctcccgcgct ggcggcgggc aactgtgtgg tgctgaaacc cgcacgtctt accccgcttt
      481 ctgtactgct gctaatggaa attgtcggtg atttactgcc gccgggcgtg gtgaacgtgg
      541 tcaatggcgc aggtggggaa attggcgaat atctggcgac ctcgaaacgc atcgccaaag
      601 tggcgtttac cggctcaacg gaagtgggcc aacaaattat gcaatacgca acgcaaaaca
      661 ttattccggt gacgctggag ttgggcggta agtcgccaaa tatcttcttt gctgatgtga
      721 tggatgaaga agatgccttt ttcgataaag cgctggaagg ctttgcactg tttgccttta
      781 accagggcga agtttgcacc tgtccgagtc gtgctttagt gcaggaatct atctacgaac
      841 gctttatgga acgcgccatc cgccgtgtcg aaagcattcg tagcggtaac ccgctcgaca
      901 gcgtgacgca aatgggcgcg caggtttctc acgggcaact ggaaaccatc ctcaactaca
      961 ttgatatcgg taaaaaagag ggcgctgacg tgctcacagg cgggcggcgc aagctgctgg
     1021 aaggtgaact gaaagacggc tactacctcg aaccgacgat tctgtttggt cagaacaata
     1081 tgcgggtgtt ccaggaggag atttttggcc cggtgctggc ggtgaccacc ttcaaaacga
     1141 tggaagaagc gctggagctg gcgaacgata cgcaatatgg cctgggcgcg ggcgtctgga
     1201 gccgcaacgg taatctggcc tataagatgg ggcgcggcat acaggctggg cgcgtgtgga
     1261 ccaactgtta tcacgcttac ccggcacatg cggcgtttgg tggctacaaa caatcaggta
     1321 tcggtcgcga aacccacaag atgatgctgg agcattacca gcaaaccaag tgcctgctgg
     1381 tgagctactc ggataaaccg ttggggctgt tctgatataa gaagctggtc gcattgggta
     1441 ttcattgcct gatgtgacgc ttacgcgtct tatcatgcct acgggaacct gaccgtaggc
     1501 cggataaggc gtacacgccg catccggcat aaacaaagcg cacgttgtta caatttacgt
     1561 tttaaaccta cgtgcgcaat aatccagact acgccaacag aagtgcgttt ctggggcgtg
     1621 ttttgtcagg agtttaatct cgcgtttcga tggtgagcgg gcgaactagg ccgtaaatca
     1681 ccattggtga tccgaggcca atcagcaata aac
//
LOCUS       DADLTD010000350         1712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.354, whole genome shotgun sequence.
ACCESSION   DADLTD010000350 DADLTD010000000
VERSION     DADLTD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.354"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..204
                     /gene="tnpB"
                     /locus_tag="JN701_004144"
     CDS             <1..204
                     /gene="tnpB"
                     /locus_tag="JN701_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7366375.1"
                     /translation="RGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQL
                     AMLLDKLDWRQPKTSRLNALTML"
     gene            235..>1712
                     /locus_tag="JN701_004145"
     CDS             235..>1712
                     /locus_tag="JN701_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7366376.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAK
                     TGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLF
                     SAEREGGALTEAGCWAHARRKVHDVYISTKSATAEEALKLIGELYAIEHEIRGLPVSE
                     RLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRL"
BASE COUNT          438 a          424 c          502 g          348 t
ORIGIN      
        1 cggggtgaca tgattaaaat cctgtgggct gatgctgatg gtctgtgcct gttcaccaga
       61 cgcctggagg aaggccagtt tatctggcct gctgtgcgtg acggcaaggt atccattacc
      121 cgctcgcaac tggcaatgct cctcgataag ctggactggc gtcagccaaa aacatcccgc
      181 cttaatgcac tgacaatgtt gtaaaaatgt catggccgga ttataaaaac ggccatgaat
      241 caaaaatacc tcattcgcat tgcagaactg gaatgccagc tccgtcagaa agaccagcaa
      301 ctgagtctgg ttgaagagac ggaggccttc ctgcgctctg cactggcccg cgccgaagaa
      361 aagatcgaag aagatgaacg ggaaatagaa catctgcggg ctcagataga aaaactgcgc
      421 cggatgctgt tcggtacccg ttctgaaaaa ctgcgtcgtg aagttgaaca ggctgaggcc
      481 ctgctgaaac aacgcgaaca ggacagtgat cgttacagtg ggcgggaaga cgatccgcag
      541 gttccccgcc agttgcgaca gtctcgtcat cgtcgcccgt taccggagca tctgccccgc
      601 gaaataaatc gcctggagcc agaagaaagc tgttgcccgg agtgtggcgg tgagctggat
      661 tatctggggg aagtcagcgc agaacaactg gaactggtga gcagcgctct gaaagtgatc
      721 cgcacagaac gggtaaaaaa agcctgtaca aaatgtgact gcatcgttga agcaccggca
      781 ccatcccgtc cgatagagcg tggtatcgcg ggcccggggt tacttgcccg cgtgttaacg
      841 ggaaaatact gcgaacacct gccactgtat cgtcagagtg aaatttttgc ccgtcagggt
      901 gtcgaactga gccgtgcatt actctccaac tgggttgacg cgtgctgcca gttaatgacg
      961 ccgctgaatg atgctctgta ccgttatgta atgaacagcc gcaaagttca cactgatgac
     1021 acaccagtaa aagtgctggc accgggcagg aagaaggcga aaacaggata tatctggacg
     1081 tatgtccggg atgacaggaa tgccggttcg ccagagcctc cggcggtctg gttcgcctac
     1141 tcaccggacc atcagggtaa acatccggag cagcacctta gtcccttccg gggtatcctg
     1201 caggcagatg cgtttaatgg ttacgatcgg ctgttcagtg ccgaacgaga aggcggcgcg
     1261 ttgacggaag caggatgctg ggctcatgcg cggcgcaaag tccacgatgt atatatcagt
     1321 accaaaagcg cgacagcgga agaagccctg aaactaatcg gtgagctgta cgccatcgag
     1381 cacgaaatac gcgggttgcc ggtgtctgaa cgcctggcgg tcaggcaaat gcagagtaaa
     1441 ccgctactga cttccctgta taagctgatg caggagaaag aacacacgtt atcgaaaaaa
     1501 tgccgtctga gagatgcgtt ccggtatatc aggaagcact gggttgcgtt gtgcaacttc
     1561 agtgatgatg gtctggctga ggcggataat aatgccgcgg aaagagcgct tcgtgcagtc
     1621 tgtctcggaa agaaaaactt tatgttcttc ggcagcgatc acggtggaga gcgtggtgcg
     1681 ctactgtacg ggctgatcgg cacctgccga ct
//
LOCUS       DADLTD010000351         1667 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLTD010000351 DADLTD010000000
VERSION     DADLTD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.355"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            13..1268
                     /locus_tag="JN701_004146"
                     /pseudo
     CDS             13..1268
                     /locus_tag="JN701_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            1561..>1667
                     /locus_tag="JN701_004147"
     CDS             1561..>1667
                     /locus_tag="JN701_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine acid phosphatase"
                     /protein_id="HAY7366377.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVL"
BASE COUNT          546 a          263 c          342 g          516 t
ORIGIN      
        1 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
       61 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
      121 ctttttgaaa acacactggt gtgttttatg cggttataca atgaactcag acgtaaagta
      181 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
      241 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
      301 tattcagggt ttcaggaggt attacatata ctatacaata tgttcaaaat gttgatattg
      361 ttagagtgtc cttgccgggc agaggttcag agtctatcac agattttaaa ggttattatt
      421 ggtatggctt tatggaatat attgaaaaca ttactgcgtg tgatgatgtt ttttctgagt
      481 attgtttaga tgatgaaaat atgagtatcc agccagaaca gataaatacg ccgggaatat
      541 ctgatttaga tactggtatt gatttgtctg gcatatcttt tattcagagt gaaattaata
      601 aaacatatgg attaaaatac gctccagtag atggcgatgg atattgtctg ttaagagcca
      661 tactggtttt aaaagaacat gagtattcat gggcgctggg cggccataag acgcaaaagc
      721 aagtatacga agagtttatt aaaattgttg ataaacaaac gatcgaggct cttgttgata
      781 cggcattcta taatctcagg gaagatgtaa agacgttatt tggcgttaat ctacaatctg
      841 acaacaaaat tcaggggcag ggtggttttt tatcatggag ttttttgtct tttaaaaaag
      901 aatttattga tagttgcttg aatgacaaaa aatgcatact gcatttaccc gaatttatat
      961 ttaatgataa caaggtacgc cttgttttag acaccgaccc agagcagagg gttaatgagg
     1021 tgaaaaattt cctcactgct ctttcagata gcatttgctc attatttatt gttaatagta
     1081 atatggcatc aatctccttg gggaatgaat ccttttcaac agatgacgac cttgagtatg
     1141 gttatttaat aaacactggc aatcattatg atgtttacct ccctcctgaa ctttttgctc
     1201 aggcttataa gttaaaaaat aaggaaatga atgcgcaact cgactattta aatcgttatg
     1261 caatttaatg gcaaaggcat atgctaaaaa ccattgttat tagtctcaca cttttttatt
     1321 ggtaaatatt gtctctgtat tggtaacgcc gcagatattc tgtttagcca caggtgcaat
     1381 tatcagcggc gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg
     1441 tttttgaaga tgtatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc
     1501 ggaaaaaagt catatttctg tcacactctt tagtgattga taacaaaaga ggtgccagga
     1561 atgaacaaaa cgctaatcgc cgcagctgtg gcagggatag ttttactcgc ttcaaacgct
     1621 caggcacaaa ccgtaccgga aggctatcag ctacagcaag tgctcat
//
LOCUS       DADLTD010000352         1648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLTD010000352 DADLTD010000000
VERSION     DADLTD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.356"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..950)
                     /gene="corA"
                     /locus_tag="JN701_004148"
     CDS             complement(<1..950)
                     /gene="corA"
                     /locus_tag="JN701_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY7366378.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1320..>1648
                     /locus_tag="JN701_004149"
     CDS             1320..>1648
                     /locus_tag="JN701_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366379.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
BASE COUNT          426 a          427 c          412 g          383 t
ORIGIN      
        1 tacaaccagt tcttccgctt aaagtacaga tacggtgcca ggcccgcgag gatcataaag
       61 ataatcgcgc cagggtagcc gaagctccac ttcagttctg gcataaactc aaagttcatg
      121 ccatagctgg aagcaacgag cgtcggcggc aggaatacca cggataccac cgagaagatt
      181 ttgatgatgc ggttctgctc gatgttgata aaacccatcg ccgcctgcat caggaagttc
      241 accttctgga acagagattc gttatgcggc agcagggatt cgatatctcg caggatttca
      301 cgcgcctgct ccagttgtcc acccggtaag cgtgctttac gtaccaggaa gttgagcgcg
      361 cgctgggtat ccatcagaca caggcgtact ttccagccga tatcttccag ttccgccagc
      421 gtggagagcg cctcatcgta ctcatcgccc tgatgccctt ccataatcac ccggctcaac
      481 tgctccaggt cgctatagat attttcaatt tcatctgcca actgttcgat tttggtttcg
      541 aacagatcca gcagcagctc gtaggcgtta ccgtctacca tcgactggct acgggcacgc
      601 atacgataca gacgaaaagc gggcagttca cgctcacgca gagtaaacag acgaccatca
      661 cggatggtaa atgccacagt ggagttaccg gcgtgatctt ccgcatcttc aaagaagaag
      721 aaggagtgaa tatgcaggcc gtcgtcgtct tcaaagaaac gtgccgatgc ttcgatgtct
      781 tccagttccg ggcgggttgc caggctctgg ccaagttcag attgtacacg cagtcgctcg
      841 tcgtcgtccg gttcgacaag atcaatccat actgcattta caaggggttg tgactcttcg
      901 acttccagcc gggtcagtcg gttattttcc agttgaaatg cgctcagcat gaccgggact
      961 cccaatgctt aaaatatcgg acagttcggt gggcacacag aaacaaattg ggtttcagac
     1021 cattaaacag cctgactcag cgcgacggga aaaattgagg tcgctgacaa ccgctaaggc
     1081 tatcagcaaa aagggatagc cttaggagtt gatcctggat gacaggatag tgagccagta
     1141 tctactgggt gtgtccaagg cgaatgtcct cttagaatga tcgtgcgcgc atgttacgcc
     1201 agcaaaattt tgccgtcaac acgcaacaga acaccacaga acaatagttg aacttcgcgc
     1261 tttttggtac tcctcaaggt cgctaacagg ataaaaaaat cccacatcca ggacctataa
     1321 tggcgcatcg gctactcatt ggtaaaggaa tgatcacctt gaatctcaaa cggatttttc
     1381 tcaccctcac cttacttccc ttgttttctg ttgccgctga tgattgcgca ctctcagatc
     1441 cgacgctgac cgtacaggcg tataccgtta atcctcaaac agagcgggtg aaaatgtact
     1501 ggcaaaaagc caatggcgaa gcgtggggaa cgttacatgc tctgctggcg gatattaata
     1561 gtcagggtca ggtgcagatg gcgatgaacg gcggcatcta tgatgaaagc tatgcgccgc
     1621 tcggtttgta catcgaaaac ggtcagca
//
LOCUS       DADLTD010000353         1638 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLTD010000353 DADLTD010000000
VERSION     DADLTD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.357"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..263)
                     /locus_tag="JN701_004150"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="JN701_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /locus_tag="JN701_004151"
     CDS             complement(378..1016)
                     /locus_tag="JN701_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366380.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1638)
                     /locus_tag="JN701_004152"
                     /pseudo
     CDS             complement(1175..1638)
                     /locus_tag="JN701_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          462 a          408 c          302 g          466 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg gctaacagtt
     1201 cccgataggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccgacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacgg
//
LOCUS       DADLTD010000354         1621 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLTD010000354 DADLTD010000000
VERSION     DADLTD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.358"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(1..1577)
                     /locus_tag="JN701_004153"
                     /pseudo
     CDS             complement(1..1577)
                     /locus_tag="JN701_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433765.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          420 a          411 c          349 g          441 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctactcctgc caatttttgg ccttcatctg atgagtccag
      301 gccagcaaag acgccacctc tttaccatcc catgcgcggg atttagcgta agccgtttgt
      361 gtcgtctggt cagtttggag aatgttgcca tgcaccgtac gccagacagt aaaggtctct
      421 gcctgaccat ttttcaccgt aatggtttcc tcacggctta acattttcac ccacttacaa
      481 ttatgtaagt agtagcctgg tttctctgcc gacagccgtt cagcaaaaat atcgacatca
      541 tcgccgaaac ctgccgttga tccccaggaa atcacaccat tatgaccaaa aaccagccca
      601 ggataggcaa atggtgtatt gccagtgaca tcataaccag caccgtgcag accaatacca
      661 taagtatacg caggcgcata ccagccaaac tgcggaccat ttaccatgat tgctttcgca
      721 tcctgggctt tgcttttgcc gatcacccac atattgctgg tcgttggata ccccgccaga
      781 ccattggcac caccctgtgc aaattgtgca gcaatagttt cccggttctt ccctgctgtt
      841 aacgccagca gtgcgccatc cgcccctttt gctggtcggt caagcattgg tgcaggtaaa
      901 tcgtagcgtg gcaacagagc tgctgtttgc gagttttgct gattaaattt aagtgggtag
      961 ctactctctt gtacggcaat agtggttggc gctgatgggt ttaccagcca tttcaactga
     1021 ttaaataccg ccatgccttg tgatacacca tatttatctt ttaaagccgt tagcagtgcc
     1081 agattatcaa tttcgctagt gctatcagag aagcggtttg ccatggtgcc cacaaatatc
     1141 atcgcgacat caaacggttc ccagcgctta ggagtaaagc caaatgtatt aaactgtttt
     1201 ggtaagagcg tctctggatt ggtatttacc ttatcaatcc aggcattcat tccatcagcg
     1261 tagccttgca gaatggacat atcctctggg gaaagggcat caatttgcgc ccggatagca
     1321 tccggccagt agttacgacg gatatcttta tcaaatttca caaaatcttt gccaagcact
     1381 tccgcgacag tcccttgagt actgcgacgt gccatttcca tctgaaaaag gcgatcttgt
     1441 gctactacat agccatagcc ataaaatagg tgccatgtat cattggcata aatatgcggc
     1501 atgccgtatt catcgcgaac aatctttatc tcacttgacg actgctcagc cagtgcaggt
     1561 aagctccaat aatacatcag ggaagcagta acacagttca cgatcatacg atttctattt
     1621 t
//
LOCUS       DADLTD010000355         1586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLTD010000355 DADLTD010000000
VERSION     DADLTD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.359"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(71..493)
                     /locus_tag="JN701_004154"
     CDS             complement(71..493)
                     /locus_tag="JN701_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366381.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVVVH"
     gene            complement(477..704)
                     /locus_tag="JN701_004155"
     CDS             complement(477..704)
                     /locus_tag="JN701_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7366382.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            781..1188
                     /locus_tag="JN701_004156"
     CDS             781..1188
                     /locus_tag="JN701_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7366383.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1391..1543
                     /locus_tag="JN701_004157"
     CDS             1391..1543
                     /locus_tag="JN701_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY7366384.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
BASE COUNT          447 a          413 c          327 g          399 t
ORIGIN      
        1 gccgaacctt cctccggata taatctgtga ttccccaatc aacagaacca gaggaggttc
       61 ggcataaaca tcaatgcaca acgacagaat cgtcggacga cccaccaccg ctgaaatgtg
      121 ctttccggta aacggcctga actgcgtcat catgcgcatc aattgccgta ctcaacgctt
      181 cctgcgccgc cagtaatgca cggcgttcca gggtatcgaa gatacagagt cggtgacgca
      241 gctcacgcgg aagaattgcc agaaccgcag ggatcagttt ctgaattttc tccctttgcg
      301 ctttcgtttc acctttcaac caacggtgat agatattctg ctgattgttc cagtccttgc
      361 ctggtaccag aggcaattcg ccgcccccct gccgcagata ttcttcagta attgcgttag
      421 cgacccacgc ctgccctttt tcggctgcca gggctaacag cactgattcg atgtgctcat
      481 gcctgatttt catgaatcac cttcccaggt cgttttgcct tacgatattc gtcataaact
      541 ttggggtcgt actgaagttc cccgccagat gcctcttgca ggcgcatcgc gcgaccttca
      601 ggaaccagtt ccccccatgc agcaatgctt gccagcctaa ctcctgcggc attggcaagc
      661 tttgttttgc tgccaaaaaa cgctatagca tcaattttca acatatcgag ctccttagat
      721 tttcctaagg aaactagatc gtagagaaac ctaagtcaag aaagattaga attccctaat
      781 atgaaaaacg aaaccttcgg tgctcgcctc ttacataggc gtaaaaaatt aaaactgtct
      841 caggccgcat taggtaagct ggtcaaagtg gctcacgtaa caatttctca atgggaaaga
      901 gatgaaacac agccggcggg gaaaagatta ttcgcactga gccaagcgct tcagtgctca
      961 ccgacttggc ttctttttgg ggatgaagat aaacaaccag gcgaaccgat cccggataat
     1021 cagccagcca ttctgacaga agatcaaaaa gagttacttc aactgttcga cgcactgcct
     1081 gagtcagagc aaaaggccct gttgtcagag atgcgtgctc gagttgaaaa tttcaacaaa
     1141 ctttttgaag aactactcaa agctcgcaaa agaagcgcaa acaaataatc cccttttttc
     1201 tccacaccct gtaataaaaa gcacaaactt tcaaacactt gtgtttttta catcaaaaaa
     1261 acttaggttt ttatacataa aatcttgact ccatgtctta ggttattcta aatttcattc
     1321 atcaagacac cgcacggtgt tctcagcaaa cagttccact accccggcgt taaggggaaa
     1381 tgaggtcagc atggatacta tcgatcttgg caacaacgaa tccctagtgt gtggcgtgtt
     1441 tcccaatcag gacggcacat tcaccgccat gacgtatacc aaaagcaaaa cgtttaaaac
     1501 cgaagctggc gcacgtcgct ggcttgccaa aaataccagc taatccattt attggattaa
     1561 ttcaatattc tcgctgtaga ggtata
//
LOCUS       DADLTD010000356         1573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLTD010000356 DADLTD010000000
VERSION     DADLTD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.360"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..174)
                     /locus_tag="JN701_004158"
     CDS             complement(<1..174)
                     /locus_tag="JN701_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7366385.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYP"
     gene            173..1312
                     /gene="queG"
                     /locus_tag="JN701_004159"
     CDS             173..1312
                     /gene="queG"
                     /locus_tag="JN701_004159"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY7366386.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRICHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(1295..1348)
                     /locus_tag="JN701_004160"
     CDS             complement(1295..1348)
                     /locus_tag="JN701_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366387.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          408 a          386 c          404 g          375 t
ORIGIN      
        1 aggatacgcc gaacgacaca cctggaatgc ggcctcgcca gcgcgaagca tcagctcata
       61 gagtgtgagc cccagcacat ctgccgcctc gcgttctccg cggcggatat cgtcggcgta
      121 ccagacggtg tgtggtatac ttacggggtt tttcttcatt gtatggtccg tcatgtcaga
      181 gcccctcgat ctcaatcagt tagcgcaaaa aattaaacag tgggggctgg aactgggctt
      241 tcagcaggta ggtattaccg ataccgatct cagcgagtcc gagcccaaac tgcaagcatg
      301 gctggacaaa caataccacg gcgaaatgga ctggatggca cgtcacggta tgctgcgcgc
      361 tcgccctcat gagttattgc ccggtacgct gcgcgtgatc agcgtgcgga tgaattacct
      421 tcctgctaac gccgcatttg ccagcacgct gaaaaacccc aaactcggct atgttagccg
      481 ttatgcgctg ggccgtgact atcacaaact tctgcgcaac cgactcaaaa agctgggcga
      541 gatgattcag caacattgtg tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat
      601 tctcgagcgc ccgttagctg aaaaagctgg gctcggctgg acaggtaagc actcacttat
      661 cctcaatcgc gaggccggtt cgttcttctt tttaggcgaa ttgctggtcg atattccgct
      721 gcccgtggat caaccagtcg aggaaggatg cggtaaatgc gtggcctgta tgacgatttg
      781 cccgaccggt gccatcgtcg agccatatac cgtcgatgct cgccgctgta tctcttatct
      841 caccatcgaa cttgaagggg cgatcccgga agagttgcga ccgttaatgg gaaaccgtat
      901 ttacggttgc gatgactgcc agcctatctg cccgtggaat cgctattcac aactcaccac
      961 agaagaggat ttcagcccgc gtaagccgct acacgcaccg gaactcattg agttattcgc
     1021 ctggagcgaa gagaagtttt taaaagtcac ggaaggatcg gcgattcgtc gaatttgtca
     1081 cctgcgttgg ctgcgtaata tcgccgtagc attaggcaat gccccttggg atgaaacgat
     1141 tttgacagcg ctggaaagtc gtaaaggtga gcacccactt ctcgatgagc acatagcgtg
     1201 ggcgattgcg cagcaaatag agagacgaaa tgcgtgcata gtcgaagtgc aattgccgaa
     1261 aaaacagcgt ctggttcggg tgattgaaaa agggttaccg cgtgacgcct gattcatcca
     1321 cagcttgtga ataaaaataa aaacgcattg caattcaaga cgaataaaat cgtcaagtga
     1381 tcgactcgac aaaacggaat cttaattttt aacttatcta tcaataggtt aaaaaaatac
     1441 tattcaccgt gcgaagtttc tttgaaatta agattacagc ctgaggctgt ggataactct
     1501 gtttacaaga gtttgtcaga gacaacgaaa accagcaata acgcatcgct gtggatattt
     1561 tattgagaga aga
//
LOCUS       DADLTD010000357         1573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLTD010000357 DADLTD010000000
VERSION     DADLTD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.361"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..90
                     /gene="yoaK"
                     /locus_tag="JN701_004161"
     CDS             <1..90
                     /gene="yoaK"
                     /locus_tag="JN701_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY7366388.1"
                     /translation="GIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            93..167
                     /gene="yoaJ"
                     /locus_tag="JN701_004162"
     CDS             93..167
                     /gene="yoaJ"
                     /locus_tag="JN701_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY7366389.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(226..474)
                     /locus_tag="JN701_004163"
     CDS             complement(226..474)
                     /locus_tag="JN701_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7366390.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(622..804)
                     /locus_tag="JN701_004164"
     CDS             complement(622..804)
                     /locus_tag="JN701_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HAY7366391.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(808..1168)
                     /gene="yeaR"
                     /locus_tag="JN701_004165"
                     /pseudo
     CDS             complement(808..1168)
                     /gene="yeaR"
                     /locus_tag="JN701_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
     gene            complement(1341..>1573)
                     /locus_tag="JN701_004166"
     CDS             complement(1341..>1573)
                     /locus_tag="JN701_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY7366392.1"
                     /translation="FFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ
                     YIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
BASE COUNT          432 a          374 c          350 g          417 t
ORIGIN      
        1 ggtattattt ttccggttgt aatctttatt acagcggtcg tatttttagc atggtttttt
       61 attggcggct atgctgcccc gggggcataa agatgaaaaa aacaacgatt attatgatgg
      121 gtgtggcgat tattgtcgta ctcggcactg agctgggatg gtggtaacgt cacctctaaa
      181 aaatagcaaa ggctgcctgt gtgcagcctt tgtgcaattt aagcgttaac ttttaatctt
      241 cctgtagata aatagcacga caatcgcacc aataacggca accacgaagc tgccaaaatt
      301 gaagccatcg actttaccaa agccaaacag cgtgctgatc catccgccga ctacggcacc
      361 gactatcccc agcaggatag tcataaagaa tccaccacca tctttacctg gcatgatcca
      421 cttcgccaga ataccggcaa taagcccaaa aataatccat gacagaatgc ccattgtttc
      481 ctcacttatc tgttttgcat tagcgggtta gtcgctgata aaaagcatag cacaacatcg
      541 ggagggcaag atttgtgacg agcatcacgg aggatttttt tgcgatggcg cagaaattgc
      601 gccatcaacg atcagtgata attaccaacc acaaacatca tgttcgtttt ccgtgtcata
      661 agaacgcacg gtattcacca gatcttttat cacttcagcc gccacttctg gcaccagcaa
      721 agtcatcggc gtctctgttt cataatcgac agaaacgcca ttactgttat tggtgacggt
      781 cacggtatac gttgctttgc ccatgattca tttcccgtta tgaatgactt tccgttgttg
      841 cgcaccttcc atcaggactt caggagccac gaagaagtca atgttgacat aagtatcgtc
      901 agtcatggct tcaatgttgt gccacttttc tggagggaac accgcaaact gccccgcttc
      961 gataaggatc acctgatcag gctctgcact gtgttcatca gcgtagccga gatatttgac
     1021 cgccccatgc ataacggaaa ggcgtgggta acccccctgg cgcgttcctt tatcaagatg
     1081 acgttcgaat tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat
     1141 gaatataatt ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa
     1201 cggtttattc cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt
     1261 ggttactttt tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc
     1321 gacaacgcgg gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg
     1381 aacatcaaac caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac
     1441 tgcgtgacaa aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc
     1501 agcgtcgtcg ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac
     1561 tgtacgaaaa acg
//
LOCUS       DADLTD010000358         1522 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLTD010000358 DADLTD010000000
VERSION     DADLTD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.362"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1189)
                     /locus_tag="JN701_004167"
     CDS             complement(<1..1189)
                     /locus_tag="JN701_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HAY7366393.1"
                     /translation="MLREVSISNDTISVKFYRNEKIECACNFLMDKDSQGYIDLSDFD
                     VTNCHFKGDVISKVSFLSSNLQHVTFECKEIENCNFTKATVDNVIFKCRRLHNVIFLK
                     TSGECVDFSQDILDTVDFSQSQLGHSNFRECQIRNSNFDNCYLYASHFTRAEFLSAKE
                     ISFIKSNLTAVMFDHVRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMID
                     TLPDNAMILKSVLAVKLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGE
                     DNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDM
                     IKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVV
                     SSQR"
     gene            complement(1192..1386)
                     /locus_tag="JN701_004168"
     CDS             complement(1192..1386)
                     /locus_tag="JN701_004168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366394.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKIMQ"
BASE COUNT          521 a          266 c          224 g          511 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aaaatcagac aggtcgatat
     1081 acccctgcga atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
     1141 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
     1201 gattttttcg ttccgttgtt ttatatttaa aaatatatca ctgatataaa taaagttatc
     1261 tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa tcaagatcta ctatattatt
     1321 tccttgaacc acatcatgtt tcatcaaaga gttattgcca ccagaatttg tgatatgacc
     1381 tatcataaaa cctctgtaat acagaaataa aataacctgg aaaagtagcc tgagcgtcca
     1441 gacactaccg gcgaaaggaa aagaatctct gataaggcat tgagataatg gacattctta
     1501 gtgaagaaga agtgaagtac ta
//
LOCUS       DADLTD010000359         1517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLTD010000359 DADLTD010000000
VERSION     DADLTD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.363"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..335)
                     /locus_tag="JN701_004169"
     CDS             complement(<1..335)
                     /locus_tag="JN701_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY7366395.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVD"
     gene            417..644
                     /gene="vapB"
                     /locus_tag="JN701_004170"
     CDS             417..644
                     /gene="vapB"
                     /locus_tag="JN701_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY7366396.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            644..1042
                     /gene="vapC"
                     /locus_tag="JN701_004171"
     CDS             644..1042
                     /gene="vapC"
                     /locus_tag="JN701_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY7366397.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(1051..>1517)
                     /locus_tag="JN701_004172"
     CDS             complement(1051..>1517)
                     /locus_tag="JN701_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HAY7366398.1"
                     /translation="ERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDV
                     PEVVSGEDVTQAEQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPG
                     ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          353 a          435 c          376 g          353 t
ORIGIN      
        1 aaatccacgg cctggttatg tgcttcaata agacgtttgt cccccccgag catggccatc
       61 atggagacac ttttgggagc ggagaaggtc aggtcgtagc cgggacgatg cctgttactg
      121 ccatcctgca tgcggcttaa atccgccccg tccggcagcc tgccctccag aaggcgggta
      181 aagacatcct tatcgacact gccctgcagc cccagttgtt cagccccctg gccggcccag
      241 cgttctccca tgctgcccag cacatagtaa ttatccttgt tggtataata attaccggca
      301 cttccggccg atctgacctg cgcgatactc atcataaacg tatatccctt tgacatatcc
      361 cggcatcaat cccacaatgg atatacacaa gacatatcca cataaggagg caaataatgg
      421 aaaccaccgt atttctcagc aaccgcagcc aggcggtcag actgccaaaa gcggttgcat
      481 tgccggaaaa cgtaaaacgc gttgaagtga ttgctgtcgg acgaaccaga atcattacgc
      541 cagccggaga gacatgggac gaatggttcg acggacacag cgtcagcgcc gattttatgg
      601 ataacaggga acagcccggc atgcaggaaa gggagtcatt ctgatgctga agtttatgct
      661 cgataccaac atctgcattt ttacgataaa gaacaaaccc gccagcgtca gggagcgttt
      721 taacctgaac caggggagaa tgtgtatcag ttcggtcacc ctgatggagc tgatatatgg
      781 tgcagaaaaa agccagatgc ctgaacgtaa tctcgctgtg atcgagggat ttgtttcccg
      841 cattgatgtt ctggattacg acgctgctgc agccacacac accggccaga taagagcaga
      901 acttgcccgt cagggacgcc ctgtcgggcc atttgatcaa atgatcgcag gtcatgcccg
      961 cagtcggggg ctgattattg tgactaataa cacccgggaa tttgaacgtg tgggcggcct
     1021 gagaactgaa gactggagct gacctgttca tcagaaatca tctcccggct caacatcctc
     1081 cccgcgttcc cggtgcacat tgatgttcac ctcttcacga cgctgcatct gctgccaggt
     1141 gtccggatga ttttcctgct gccatgcctc ataagcggcc atatccacca cttcaccgga
     1201 ttcactgatc ccgggtggca gttgctgttc catctcttct tccggtttca ttttcagctc
     1261 ctgctcgatc ccccctgccg gaacattcac acctgaatct gacttcttat cgttgatgac
     1321 aggagacacc ggctgttgcg gctgttcagc ctgagtcacg tcttctccgg aaacaacctc
     1381 cgggacatcc ggttcgaaga ggctggccat ctgacgacct tctgcttccc ttgcggcaag
     1441 tacggcactc agacggttct ccatttccgg gttgatgtca cgcggaataa actccggagc
     1501 gaccttcggt cgttcct
//
LOCUS       DADLTD010000360         1509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.364, whole genome shotgun sequence.
ACCESSION   DADLTD010000360 DADLTD010000000
VERSION     DADLTD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.364"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            86..607
                     /locus_tag="JN701_004173"
     CDS             86..607
                     /locus_tag="JN701_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366399.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
     gene            667..1155
                     /locus_tag="JN701_004174"
     CDS             667..1155
                     /locus_tag="JN701_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7366400.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSAITDKSSYNAIEWPVSPEG
                     SS"
     gene            1179..>1509
                     /locus_tag="JN701_004175"
     CDS             1179..>1509
                     /locus_tag="JN701_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366401.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFEEMV"
BASE COUNT          418 a          327 c          402 g          362 t
ORIGIN      
        1 gtatcggcgt tcagaacgcc ctggggggaa ttcaattgct ttcggggata acgatacagg
       61 tataaaacaa aacggcgacg gcattctgga tgtttatgcg aatggacagc atgtatttcg
      121 tttccagaat ggtgtggcga tagcgttaaa aaatattcag gccggaaatg ctaaaaaatt
      181 cacgttatcc agcgccaaca actccacgaa aaatgcagcg ttttatttgt ggggtaatcc
      241 atccaggcct gttgttgcag agcttggtga tgattccggc tggcatttct acagccagag
      301 aaataccgat ggtagtatca cattcgctgt aaacggacag gtaattccgt taaattacgg
      361 aaacttcgat gcccgctata aacatcgaac tgagggggta caggatgtgc ggtatggtta
      421 tgaaatgtat tacaaccctg gcagcaacac cgtttcatgg acgtttcgct caccttcggg
      481 acacgggttg tcagggatat cgatatcgga taccggccgt aactcagcgg ataacgtcaa
      541 cggtgtgtat taccgaccgc tgcaaaaact gattaatggc atctggtata acgtagcgag
      601 tatttaacaa tgttgcattt aaaaaatatt acggcaggta atccgaaaac cgcggaacaa
      661 tatcagctga caaaacagta tgatgtcacc tggctttttt cggaagacgg caaaaactgg
      721 tatgaagaac agaagaactt tgccagtgac accataaaaa tggtttacac cggagacggg
      781 cgcgtggtgt gggtcggtaa ggatgtgaca ggcattgaac cccgtaacgc cagtgttatt
      841 gaagttcctg atattaccgc caaccgccgc attaccgcgc cgggttactg gttttaccgc
      901 aatgatgaat ttgtctttga ctacagactc aaagcggaag atgagcgtga tgcccttctg
      961 gctcaggtca gtgcccggac aggggaatgg gaagaagacc tgctgctggg gctaatcagc
     1021 gacgaagacc gggaaaaact gaaagcgtac cgtatttacg cgaaatcgct gcaggcgatg
     1081 gatttcagcg ccatcactga taaatcctca tacaacgcca ttgaatggcc cgtctctccg
     1141 gaaggttctt cctgatttaa tttatcgcga gaaaaactat gtctgtagtg atatcaggtg
     1201 cgctgactga tggcgcaggc atccccatgt ccggatgcca gattattctg aaatcccgtg
     1261 taaacacctc agaagtggtg atgcgtacca gggctgatgt agtgaccgga aataacggtg
     1321 aatattcgtt tgaggcacag gtcggaaaat attgcgtgta tctgaaacgg gactggcgcg
     1381 acgagtactg tgttggcgac attgctgtat acgacgactc aaagcccggc acgctgaatg
     1441 actttctgac tgcccccgat gaaggcgacc tgaagccgga tgtcgtcaaa cgctttgagg
     1501 aaatggtgg
//
LOCUS       DADLTD010000361         1476 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLTD010000361 DADLTD010000000
VERSION     DADLTD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.365"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            8..295
                     /locus_tag="JN701_004176"
     CDS             8..295
                     /locus_tag="JN701_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7366402.1"
                     /translation="MQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGYYWD
                     GGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(448..1224)
                     /gene="yaaA"
                     /locus_tag="JN701_004177"
     CDS             complement(448..1224)
                     /gene="yaaA"
                     /locus_tag="JN701_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY7366403.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1294..>1476)
                     /locus_tag="JN701_004178"
     CDS             complement(1294..>1476)
                     /locus_tag="JN701_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HAY7366404.1"
                     /translation="ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPD
                     IGRQLSPDAWDDVSQE"
BASE COUNT          379 a          379 c          369 g          349 t
ORIGIN      
        1 gaaaaagatg caatctatcg tactcgcact ttccctggtt ctggtcgctc ccatggcagc
       61 acaggctgcg gaaattacgt tagtcccgtc agtaaaatta cagataggca atcgtgataa
      121 tcgtggctat tactgggatg gaggtcactg gcgcgaccac ggctggtgga aacaacatta
      181 tgaatggcga ggcaatcgct ggcacccaca cggaccgccg ccaccgccgc gccaccataa
      241 gaaagctcct catgatcatc acggcggtca tggtccaggc aaacatcacc gctaaatgac
      301 aaatgccggg taacaatccg gcattcagcg cctgatgcga cgctggcgcg tcttatcagg
      361 cctacgtgaa ttctgcaata tattgaatct gcatgctttt gtaggccgga taaggcgttc
      421 acgccgcatc cggcattgac tgcaaactta acgctgctcg tagcgtttaa acaccagttc
      481 gccattgctg gaggaagctt catcaaagaa gtaaccttcg ctattaaaac cagtcagttg
      541 ctctggtttg gtcagccgat tttcaataat gaaacgactc atcagaccgc gtgctttctt
      601 agcgtagaag ctgatgatct taaatttgcc gttcttctca tcgaggaaca ccggcttgat
      661 aatctcggca ttcaatttct tcggcttcac cgatttaaaa tactcatctg acgccagatt
      721 aatcaccaca ttatcgcctt gtgctgcgag cgcctcgttc agcttgttgg tgatgatatc
      781 tccccagaat tgatacagat ctttccctcg ggcattctca agacggatcc ccatttccag
      841 acgataaggc tgcattaaat cgagcgggcg cagtacgcca tacaagccgg aaagcattcg
      901 caaatgctgt tgggcaaaat cgaaatcgtc ttcgctgaag gtttcggcct gcaagccggt
      961 gtagacatca cctttaaacg ccagaatcgc ctggcgggca ttcgccggcg tgaaatctgg
     1021 ctgccagtca tgaaagcgag cggcgttgat acccgccagt ttgtcgctga tgcgcatcag
     1081 cgtgctaatc tgcggaggcg tcagtttccg cgcttcatgg atcaactgct gggaattgtc
     1141 taacagctcc ggcagcgtat agcgcgtggt ggtcaacggg ctttggtaat caagcgtttt
     1201 cgcaggtgaa ataagaatca gcatatccag tccttgcagg aaatttatgc cgactttagc
     1261 aaaaaaagag aatgagttga tcgatagttg tgattactcc tgcgaaacat catcccacgc
     1321 gtccggagaa agctggcgac cgatatccgg ataacgcaac ggatcaaaca ccgggcgcac
     1381 gccgagttta cgctggcgta gataatcact ggcaatggta tgaaccacag gcgagagcag
     1441 taaaatggcg gtcaaattgg taatagccat gcaggc
//
LOCUS       DADLTD010000362         1460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLTD010000362 DADLTD010000000
VERSION     DADLTD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.366"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="JN701_004179"
     CDS             complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="JN701_004179"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY7366405.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHL"
     gene            1160..1441
                     /locus_tag="JN701_004180"
     CDS             1160..1441
                     /locus_tag="JN701_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366406.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          422 a          321 c          324 g          393 t
ORIGIN      
        1 taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca atggctgcaa
       61 ttgggcgttt tgtcgaataa actgtcgcat ataaatagcc atttctttgt acggttaaat
      121 ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa
      181 aggttttcgt caccgtatca tacacacccc attgcaatgt cggatcatca gacatcactt
      241 cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata taggtcattt
      301 tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta ggttcacgct
      361 tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat gagcataaaa
      421 aatggttatc acgggcatag atcaattcat taatataaag atagccacga ataatattca
      481 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
      541 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
      601 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
      661 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
      721 gatgactcat tgctatcaat ttcctgcctg tttatacccg gccattgtaa ggtgaaaacc
      781 ctgggtaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga
      841 aattgatacc tttctaatcg tcacctggat gaaaataatt cattgatatt caaaattaat
      901 ttcctacagg aatgcagagg cggcgggaag atatgccctt aatgccactg aaaaaccaca
      961 taatctgtac agcaataaaa gtcacggcct aatatggcta acaaacaatc aattgtgata
     1021 tatttcacaa aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
     1081 gatagccaga gtgtaccacg cctcccgtga acaacgccgc gctgtccagg gcatcggctc
     1141 ttttcagagg aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca
     1201 acttcttagt gccgtaatga tgtttctggc cagcacactc aacatccgtt ttcgtcggtc
     1261 tgattatgtc gggcttgcgg tgatcagcag cggtctgggc gttgtttccg cctgttggtt
     1321 cgcaatgggg ttgcttggca tcacaatggc ggacatcacc gctatctggc acaacatcga
     1381 gtcggtgatg atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacctg
     1441 atacgtagaa gcctccataa
//
LOCUS       DADLTD010000363         1447 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLTD010000363 DADLTD010000000
VERSION     DADLTD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.367"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..194
                     /locus_tag="JN701_004181"
     CDS             <1..194
                     /locus_tag="JN701_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7366407.1"
                     /translation="TAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSV
                     GLAAPVERLLQQLRTGAPV"
     gene            222..497
                     /locus_tag="JN701_004182"
     CDS             222..497
                     /locus_tag="JN701_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY7366408.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            562..>1447
                     /gene="mltC"
                     /locus_tag="JN701_004183"
     CDS             562..>1447
                     /gene="mltC"
                     /locus_tag="JN701_004183"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7366409.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAV"
BASE COUNT          380 a          362 c          374 g          331 t
ORIGIN      
        1 tgaccgcgtt tcggcatacc ttcagccatt tccacttaga tattgtgcct atgtggcttc
       61 ccgtgtcgtc attcaccggc tgcatggatg aaggcaatgc gctctggtat aacttagcgc
      121 aaccgccgtc agttggcctg gcggctcccg tggagcgttt gttacagcag ttacgcactg
      181 gcgcgccggt ttagcgcgtg agtcgataaa gaggatgatt tatgagcaga acgatttttt
      241 gtactttcct gcaacgtgaa gcagaaggtc aggattttca gctgtacccc ggcgagctgg
      301 gaaaacgcat ctataacgag atctccaaag aagcctgggc gcagtggcag cacaagcaaa
      361 ccatgctgat taatgaaaag aaactcaaca tgatgaatgc cgagcaccgc aagctgcttg
      421 agcaggagat ggtcaacttc ctgttcgagg gtaaagaggt gcatatcgag ggctatacgc
      481 cggaagataa aaaataaaaa cagtgccgga gcacgcctcc ggcaacttgc ataaaaacaa
      541 acacaacacg cacccggaat gatgaaaaaa tatctcgcgc tggctttgat tgcgccgttg
      601 ctcatctcct gttcgacgac caaaaaaggc gatacctata acgaagcctg ggtcaaagat
      661 accaacggtt ttgatattct gatggggcaa tttgcccaca atattgagaa catctgggga
      721 ttcaaagagg tggtgatcgc cggtcctaag gactacgtga aatacaccga tcaatatcag
      781 acccgcagcc acatcaactt cgatgacggt acgattacta tcgaaactat cgccgggaca
      841 gaacctgccg cgcatttgcg ccgggcaatt atcaaaacgt tgctgatggg tgacgatccg
      901 agttcggtcg atctctattc cgacgttgat gatattacga tttcgaaaga acctttcctt
      961 tacggtcagg tggtggacaa caccgggcag ccgattcgct gggaaggtcg cgcaagcaac
     1021 ttcgcggatt atctgctgaa aaaccgtctg caaagccgca gtaacgggct gcgtatcatc
     1081 tacagcgtca ccattaacat ggtaccgaac caccttgata aacgtgcgca caaatatctc
     1141 ggcatggtcc gccaggcgtc acggaaatat ggcgttgatg agtcgctgat tctggcaatt
     1201 atgcagaccg aatcttcctt taacccgtat gcggtcagcc gttccgatgc gctgggatta
     1261 atgcaggtgg tacaacatac tgccgggaaa gatgtgttcc gctcgcaggg gaaatccggc
     1321 acgccgagcc gcagtttctt gtttgatcct gccagcaata ttgataccgg caccgcgtat
     1381 ctggcgatgc tgaacaatgt ttatctcggc ggaattgata acccaacatc gcggcgttat
     1441 gccgtca
//
LOCUS       DADLTD010000364         1408 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLTD010000364 DADLTD010000000
VERSION     DADLTD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.368"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..124)
                     /gene="ariR"
                     /locus_tag="JN701_004184"
     CDS             complement(<1..124)
                     /gene="ariR"
                     /locus_tag="JN701_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAY7366410.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNL"
     gene            complement(153..425)
                     /locus_tag="JN701_004185"
     CDS             complement(153..425)
                     /locus_tag="JN701_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAY7366411.1"
                     /translation="MKTSDNERIKYEITGQVVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(468..704)
                     /locus_tag="JN701_004186"
     CDS             complement(468..704)
                     /locus_tag="JN701_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAY7366412.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANWPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1018..>1408
                     /gene="bluF"
                     /locus_tag="JN701_004187"
     CDS             1018..>1408
                     /gene="bluF"
                     /locus_tag="JN701_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY7366413.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFR"
BASE COUNT          411 a          289 c          253 g          455 t
ORIGIN      
        1 ttaaattggt gacagcctgc cctaacactg ctgactcttc ttctaacaaa ttacccgaac
       61 tgcgaaagta acttgctaac gctttatcag ttattgcatt atgaattgta gtatcttcaa
      121 gcatggtaac ctctcatctt acttatgaaa ttttaatgta ttctgtttat tttcttacca
      181 ttgaacatac ttttattgtc agctgtcgct gttcttcggt ttggccctcc tgaatcacta
      241 ttgctaaaat cacgaattat gctgtcaatt aaatcgcgtc ggaaagcatc ttcctcagcc
      301 gatttcatta cgagtaattg cttaataaga gtctgcaatg aaaaatttat cttcatgcgc
      361 agtatctgga gcaccacctg gccagtaatt tcatatttta tacgttcatt atcagatgtc
      421 ttcataacga cttcatagct aacctgttaa ataatcaggc tgttgcatta ttcaaaaagc
      481 aacccaatta gtgcattata atgtttctgt tcctcttccc cagtcgcgtg ttccagtcgg
      541 caaagaagtt tggcacaaag cgactttcga ctcagattac gtccatcttt caaaatctca
      601 gtcacaattt ccccgagagt ttcctgctga gtaggccagt tagcttttgc aaagtaacga
      661 gtgattgctc ccgcagaatc taaagtcact gaattttgat gcatgctacg cctctgttaa
      721 aaatgttaat caatgttaag tgaggtaaca atagcaaact tgtacggaat atgtgtacag
      781 aatgataaat taattttttg caattagtgc caatacatat aaaacaatgt gttatgataa
      841 aacatattgc atcatttaac tgacaatcta tacgagattt ttcgccaact agtgttcaac
      901 tttttccatt ttttatcaat gtaaagaaac ggttatgttt tggtattaaa agaaagacac
      961 aagaagatat gtctgttacc gtcttactct cgcctcaccc attaccctgg attgttaatg
     1021 cttaccaccc ttatttatcg tagccatata cgtgacgacg aacctgtcaa aaaaatcgaa
     1081 gaaatggttt cgatagcaaa tcgcaggaac atgcagtctg acgtaacagg gatcttactg
     1141 tttaatggtt ctcatttttt ccagcttctg gaaggtccgg aagaacaggt taaaatgata
     1201 tatcgggcta tatgccagga tccacggcac tataatattg ttgagctgct gtgcgattac
     1261 gcgcctgctc gccgttttgg caaagcggga atggaattat ttgatttgcg cctgcacgag
     1321 cgagatgacg ttttacaggc cgtattcgac aaaggcacat caaaatttca gctaacttat
     1381 gatgacagag cgctacaatt ttttcgta
//
LOCUS       DADLTD010000365         1371 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLTD010000365 DADLTD010000000
VERSION     DADLTD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.369"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..1064
                     /locus_tag="JN701_004188"
     CDS             <1..1064
                     /locus_tag="JN701_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7366414.1"
                     /translation="AETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQR
                     FVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPGG
                     WLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVGL
                     SSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLGC
                     QAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELTM
                     AADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAVK
                     TGKEAAEAIHHYLEGACSC"
     gene            1058..>1371
                     /locus_tag="JN701_004189"
     CDS             1058..>1371
                     /locus_tag="JN701_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY7366415.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VL"
BASE COUNT          348 a          328 c          380 g          315 t
ORIGIN      
        1 ctgccgagac aattcgcgaa aataatgccc tcggtgccgt ttgtgccaga gtgtgcccga
       61 cggagaaatt atgccaaagc ggttgtaccc gtgccggtgt tgatgctccc attgatatcg
      121 gccgcttaca gcgttttgtt actgattttg aacaacaaac cgggatggaa atttatcagc
      181 ccggtactaa aacgctcggc aaagtcgcga ttattggcgc aggtcctgcc ggattgcagg
      241 ccagtgtgac actgacaaac cagggttatg acgtcacgat ctatgagaaa gaagcacaac
      301 ccggtggctg gttgcgtaac ggcattccgc aattccggtt accgcaatca gtactggatg
      361 cagagatcgc ccgtattgaa aaaatgggcg tgaccattaa gtgcaacaac gaagtcggta
      421 acacactcac ccttgagcag ctgaaagcag aaaaccgcgc ggtactggtc accgtggggt
      481 tatcaagcgg ttccgggcta ccgctgtttg agcatagtga cgttgagatt gccgtcgact
      541 tcttgcaacg tgcacgacag gcgcaaggcg atatcagcat tccacaaagc gcattaatta
      601 tcggcggcgg tgatgtcacg atggacgtag ccagcacgct gaaagttctc ggctgtcagg
      661 cggtaacttg cgtagcgcgt gaagagttag atgagtttcc ggcaagcgaa aaagagttta
      721 ccagcgcccg ggagctgggc gtttcgatca tcgatggatt cacgccagta gccgtggaag
      781 gcaataaagt cacgtttaag catgtacggt tatcgggcga actgacgatg gcggcagata
      841 aaattattct cgccgtcggt cagcatgcca gactagatgc ctttgcggag ttagagccgc
      901 agcgtaacac catcaaaaca caaaattacc agacccgcga cccgcaagtc tttgctgctg
      961 gcgatattgt tgagggtgac aaaaccatgg tctatgccgt gaaaaccggg aaagaagccg
     1021 ccgaggcgat tcatcactat ttagagggag cttgctcatg ttaacgaaag atctttcgat
     1081 tactttttgc ggcgtgaagt ttcccaaccc gttctgcctc tcttcttcgc cggtaggcaa
     1141 ctgctatgag atgtgtgcca aagcctacga cacaggttgg ggcggtgtgg tgtttaaaac
     1201 gatcggcttt tttatcgcca acgaagtctc gccgcgtttt gatcatctgg tgaaagaaga
     1261 taccggtttt atcggcttca aaaatatgga gcagattgcc gagcatccgc tggaggagaa
     1321 tctggccgcc ctgcgtcggc tgaaagaaga ttacccggac aaagtattga t
//
LOCUS       DADLTD010000366         1369 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLTD010000366 DADLTD010000000
VERSION     DADLTD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.370"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..481)
                     /locus_tag="JN701_004190"
     CDS             complement(<1..481)
                     /locus_tag="JN701_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7366416.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAEFGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(472..774)
                     /locus_tag="JN701_004191"
     CDS             complement(472..774)
                     /locus_tag="JN701_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7366417.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1046..1318
                     /locus_tag="JN701_004192"
     CDS             1046..1318
                     /locus_tag="JN701_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366418.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          403 a          347 c          291 g          328 t
ORIGIN      
        1 agactttata aaacaaggta ttaggtgcca caactgactg aacaaaacca aaacgctcat
       61 aaaatgctct ggcgtcatca tcaattgcat gtaccataac agccttaatg ccaacctgat
      121 cagccagatt gacaacccgg tgaatggcat cacttagtag ccatttaccg aaaccatgcc
      181 cctggataca aacatcaaca gccagacgcc ctaacagaac aacagggaca ggattgggca
      241 tgttatgccg caaactgcga ccaaactcgg catgagtgac ggaaccagta gctaatgagt
      301 agtatcccac aacacgtaaa gtatcctcca agcaaataac gaacgtgcga gaagcattga
      361 gcatctggtt tttcatagcc cgaccacgta accagttact tagcacctca tttccacagt
      421 caaatggctg aagtatatgc tcatccgtca acaatgctgg cgcggtgaca tttatttcca
      481 ttcaggcttt accgccatca gacgttcacg cccttctgca ttctgcacag gagcttccag
      541 ttgcgtaata aacgaatcat aacgttcgtt atccagcaca aataaacgtt gatccaggat
      601 aatgttttta gcttcctggt acgccagttc catgatgaaa tcggtacggt tcttattgac
      661 caagctcgca gcataatcaa taacggctcg ttcctcatca gttgcccgga tgttaatctg
      721 atgttctcgc ggtgttttac ggacgcttgc agctgtagac ataaaaacct ccattggtaa
      781 caatttgcta tgcaattaat gttatcgcaa atggtaacag aacgctatac agaatcactt
      841 acgggaaaag cgatcccctg ataaaaacaa tggcagagac ctgaacattg tgacgatgat
      901 ctgtgtcggc gcacaaatca tgtctgatta cggccgttta atgtgatttt gataatgaaa
      961 tgcctgaacc acggcagcct ggcggctgca aatcggggtg tggcagaata tgagctttca
     1021 gagtaaccat ccagaatgag ttcccatgtc acacaacctg gagcaccaga aagtccacac
     1081 cagaatggtg aaagaggttc tgaaagccgt cgccagagcc aataatcacc cttataagtc
     1141 cgtatttgca gactttatca ccggtcatcc ctcctgcacg gtctgtttct gggaaacctt
     1201 ccacaaaatg tacccggatt caccgtacga atacgtcacg ttctgccata cctgccgtcg
     1261 ttttgattta tacgaaacag aagcggagat gaaggcggat gacccgaagt ggtggtgatt
     1321 ttcagccccg accgctgaaa cgcctgttca cagccaacca gtgctggac
//
LOCUS       DADLTD010000367         1346 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLTD010000367 DADLTD010000000
VERSION     DADLTD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.371"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(357..623)
                     /locus_tag="JN701_004193"
     CDS             complement(357..623)
                     /locus_tag="JN701_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366419.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(805..1203)
                     /locus_tag="JN701_004194"
                     /pseudo
     CDS             complement(805..1203)
                     /locus_tag="JN701_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          349 a          273 c          278 g          446 t
ORIGIN      
        1 taataggggc accccccaaa gccgtctatg ggaaggagtc tgctgttatt ccagagaatg
       61 gtaattcagt catatatttc tccatctatg acttgtcaat accatcctga tatttgtggg
      121 tcagcaatat attctactaa aaggtattta aggtcattgc gtccctgcac tcgaataatg
      181 gtgaagaaag catgatgaat atatcacgat aacgttcagt atgtatcgag gatagatgga
      241 gttactcctc tctgaaatag aattactgct ctactttttc tgtttccaga aattattagt
      301 aacaaaccct gatatattct tttgaaaatg gagcttaaac gagtctgaaa ttaacttcag
      361 atattagaat gccgttggct ttgtttttca acaattccta cttcatttat ctttgtcaac
      421 cattgcattt ctgtagcagt gaatttcaac gttttcgtcc ggttggtagc aggtatttgt
      481 aattcgtata tagctgcttt tttattgaaa acagtatatc catctgtagt aacagtagaa
      541 gataataata caggatttga cacttcaagg atgatatttt cttgtttttg tggaaggcaa
      601 gggaatatag tttgtgtaag catattgctt ccttatgtta tattttagta aagaatttat
      661 tttttacatt atagtcggct aaatgaagtt tactgtaaaa aaacgatact tttttcgtaa
      721 gcgatgagat gtggagccag aaaaatggct gcgttacgtc attgaactgt ccttgaccca
      781 cgatattcag acagtcgttg tatcttaagt tgacattacc cgctgacgtc gatattccct
      841 tggtgagaga taacccaacg cactatgcgg atggttttca ttgtaatgct cgatcgccac
      901 tgcaaggtta tgcaatgccg ttcttggttt cggcatgaac gcgatgcagt cttccttcat
      961 cgttttcacg aaccgttctg ctattccatt actctgaggg ctactgactg ccgtcgtgca
     1021 gggctccaga ttcaactctc tggcgaactg ccgtgtttca tgagccgtat aagccgaacc
     1081 gttatcggcc actggattgg ccgttccggt cctttatcgc catatcgctg acatgacatc
     1141 ctgcaccgtt gctttgtcat aatctcccgt gcctgctgcc cagtctatgg cttcacgatc
     1201 gcaatatgga acgtaacgct gtcatcggga gaatcgtcaa tgcggcattc ggttaacgct
     1261 gatatgactt tccgtgtcaa accggaatct ctaattttga cgattttctt tttaggttta
     1321 attccctgag ggcgacgtag cagtaa
//
LOCUS       DADLTD010000368         1343 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLTD010000368 DADLTD010000000
VERSION     DADLTD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.372"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            86..1005
                     /locus_tag="JN701_004195"
                     /pseudo
     CDS             86..1005
                     /locus_tag="JN701_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            1005..>1343
                     /locus_tag="JN701_004196"
     CDS             1005..>1343
                     /locus_tag="JN701_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7366420.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLG"
BASE COUNT          380 a          302 c          326 g          335 t
ORIGIN      
        1 tttagggtct aatcaactta atgatcaata tctatagtga tatcaccaac cgttaaaacg
       61 aaccgacaac tccatgagga acggaatgat gaataataaa gtcagcttca ctaacagcaa
      121 taatccaacc atctctttgt ctgcagtgat ctatttccct ccaaaatttg atgaaacccg
      181 tcagtatcag gcaattgtgg tatctcaccc aggtggggcg ttaaagaaca aacggccgga
      241 acctatgcca aaaaactggc ggaaaaggga tttgtcacta ttgcttatga cgcatcttat
      301 caaggtgaaa gtggcggcga gccgcgtcag ttagaaaacc catacattcg tactgaagac
      361 atcagtgccg tgattgatta cctgacaacg ctttcttatg tcgacaatac ccgaattggt
      421 gcgatgggaa tttgtgccgg tgcaggatat acagccaatg ctgcaattca ggatcgccgc
      481 atcaaggcca tcggtaccgt tagtgcggtc aatattggtt cgatgttccg taatggttgg
      541 gaaaacaatg tgaaatctat tgatgccctg ccatacgttg aagcgggttc aaacgcccga
      601 accagcgata ttagcagcgg agaatatgcc gttatgccac tggcaccaat gaaggagtcc
      661 gatgcgccga acgaagaact gcgtcaggcc tgggagtatt accacacccc tcgcgcgcag
      721 tatccaacag caccgggtta cgctactttg cgcagcctta accagattat tacctttgat
      781 gcttaccata tggcggaagt gtacctgact cagccgatgc agattgtggc gggcagccag
      841 gcagggagca aatggatgag tgacgatctg tacgatcgag catcaagcca agataaacgc
      901 tatcacattg ttgaaggcgc aaaccatatg gatctttatg acggcaaagc ctatgtggct
      961 gaagcaattt cagtattagc tccgttcttt gaggaaactc tgtaatgaaa catattcaaa
     1021 tccgcaatag tgatatggcc tggcatattg ctgccaatat tcagtttcca ccaaattttg
     1081 atgagtcaaa acagtatccg gcgattatca gcgtccatcc ttttggcagt tgtaaggagc
     1141 agacgtctgg caatatctat ggcaaagcat tggctgaaaa aggctatctc gttctcgctt
     1201 atgacgccag tttccaggga gaatctggtg gggagcctcg ctggatagaa gatccgacac
     1261 agcgcgtaga ggatattagc cgtgttatcg attatgcagt aactctgcca tatgttaatg
     1321 ctgaacgtat tggcgtgctg gga
//
LOCUS       DADLTD010000369         1333 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLTD010000369 DADLTD010000000
VERSION     DADLTD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.373"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..173)
                     /locus_tag="JN701_004197"
     CDS             complement(<1..173)
                     /locus_tag="JN701_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366421.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(227..427)
                     /locus_tag="JN701_004198"
     CDS             complement(227..427)
                     /locus_tag="JN701_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY7366422.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALS"
     gene            553..1122
                     /locus_tag="JN701_004199"
     CDS             553..1122
                     /locus_tag="JN701_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HAY7366423.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFECKIEDIEIIGKIISLTVKYVR"
     gene            1138..1329
                     /locus_tag="JN701_004200"
     CDS             1138..1329
                     /locus_tag="JN701_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366424.1"
                     /translation="MLGKVFFVVLSCSLLLSPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
BASE COUNT          398 a          240 c          299 g          396 t
ORIGIN      
        1 agccagattt ctggtgcggt taataaatga ggctgagctg ggttcagctt gttccgcaga
       61 atctgcacat tcatgcctgc acgttctgcc agttgcacca gattgtggcg cagtgcaaat
      121 gcacgacagg cttcatcaaa atgtggatgt ttggaaactt ggtaatcaaa catggtcaat
      181 gcctctgatg tatttcagaa tcgaactaat taaggtttag attgcattat gaaagcgcat
      241 caacggtcat tgctgctatg ttgatcatca ctttttcgcg ttttttatct ttgcgcagac
      301 ggtgacggat aaggcgtcca tcagccaaca tgtcattgat ggtatcgatg gatagccctg
      361 tcagctcgct atagcgttca atagtcacat gaggcgtggt aagagtgatt gaaatgttag
      421 gtctcatgat gcaacattcc tcgtttaatg atgattaatc aggacgaata cggatcgttt
      481 gtattttgtg aacaccataa acatacgatt gcacagtgaa atcgtcaaga taaaagttca
      541 cttggagtga ccatgaattt ggagaaaggc ggacgaggcg ccatagagcg catggtagaa
      601 gcttatggat tcaagactcg acaggcgttg tgcgatcatt taggaatctc taaaagtaca
      661 ctcgccacac gctacatgcg tgactcattc ccagcagaat gggtaatcca gtgcgccctt
      721 gaaacgggca cctcgcttaa ttggctcaca accggacatg gttcaaagca aacttcaggt
      781 aatacaaata ctatggaagt tgctaaatat gtattatctg atggtgcctt gcgtgaagac
      841 ggtttttata tttttgataa gggatttcta ccctctacgt ttaaaaaacc ttttgtcatc
      901 acagataaca attctgaatt tatttgtgat aaagaatttg atgacatacg tgatggtaaa
      961 tgggtaataa gtattgatgg cgaagtaacg atccgtgaca ttactcgttt acccggtgga
     1021 agaatcttcg tcgagggtgg aaacagagcc ttcgaatgta agatagaaga cattgaaata
     1081 attggtaaaa ttataagttt aacagttaaa tatgttagat aataccggga ggaaactatg
     1141 cttggtaaag tattttttgt ggttttatca tgctctttgt tattaagccc actaactacc
     1201 tatgctaaaa attatccttg ttctgggaaa aagggaggtg tctctcactg tacctccgat
     1261 ggaaaatttg tttgcaatga tggaactatt agtaaatcca aaaaaatctg tactaaaaac
     1321 tcacgataac ttt
//
LOCUS       DADLTD010000370         1327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLTD010000370 DADLTD010000000
VERSION     DADLTD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.374"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(170..499)
                     /locus_tag="JN701_004201"
     CDS             complement(170..499)
                     /locus_tag="JN701_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY7366425.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(671..>1327)
                     /locus_tag="JN701_004202"
     CDS             complement(671..>1327)
                     /locus_tag="JN701_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY7366426.1"
                     /translation="TGEIDTALWRSGDVLLGSLLAMLFTGIWPQRAFIHWRIQLAKSL
                     TEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKETRIPKSIYEGIQT
                     INRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPV
                     FANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK
                     "
BASE COUNT          326 a          310 c          311 g          380 t
ORIGIN      
        1 agtgcggtta ataacaatgg agttacagaa ctttttgcct tgctgcatac agaagatgtc
       61 tgtcgctaat ttcggtaagt tatcagatgg gaatgatgac ggtgatacac agaaaagaat
      121 caatgagcaa tgagggttgc caggcaaccc tcattgaata aaacaggaat tacttcgctt
      181 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      241 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      301 tggcgatgat gccctggatt gcttcaacgc tcatccccgg cttgatgtaa tcgctcaggt
      361 tgtccagcag caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      421 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      481 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      541 aaccgctatc cgttagagcc aacattactc atagctgccg cagatgacaa tgcttttatc
      601 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctgt
      661 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      721 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccaca accttcaggt
      781 catggtggtt attgagcaac tgacgcagct cttccactgc atcgttcaat ttttccgtat
      841 tggcaaaaac cggctgcgga ttaccttcgt acaacgcatg aacaaggctc agcagtattt
      901 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg
      961 ggcgcgtggc ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa
     1021 tggtctggat accttcatat atcgattttg gaatacgggt ttctttgctg gcgggcgcaa
     1081 tcagcccacg cattttcacg gcatcggtca ggagtttttg tagatggctt tccagacgtg
     1141 ggcgttcgag taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac
     1201 ttttcgccag ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca
     1261 acattgccag taaagagccg aggagcacat cgccgcttcg ccataacgcc gtatcaattt
     1321 cacctgt
//
LOCUS       DADLTD010000371         1316 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.375, whole genome shotgun sequence.
ACCESSION   DADLTD010000371 DADLTD010000000
VERSION     DADLTD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.375"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..118)
                     /gene="cobS"
                     /locus_tag="JN701_004203"
     CDS             complement(<1..118)
                     /gene="cobS"
                     /locus_tag="JN701_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="catalyzes the formation of adenosylcobalamin from
                     Ado-cobinamide-GDP and alpha-ribazole; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY7366427.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPL"
     gene            complement(115..659)
                     /gene="cobU"
                     /locus_tag="JN701_004204"
                     /pseudo
     CDS             complement(115..659)
                     /gene="cobU"
                     /locus_tag="JN701_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            complement(793..1230)
                     /locus_tag="JN701_004205"
     CDS             complement(793..1230)
                     /locus_tag="JN701_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAY7366428.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
BASE COUNT          370 a          360 c          263 g          323 t
ORIGIN      
        1 ttaaaggaaa agtaataata ccgcgagaat aatgctcgaa atccagtccc tgggaccagc
       61 gacgcggtac aggcagacgc gtaataaaag agagcatcgc ccaaaataat ttactcattt
      121 gatttttact ccaatacccg aaaccaccaa ccatacttca tttgccgcag cggccagctg
      181 ctgatttacc cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct ccggcacaat
      241 ccccattccc acttcgttag tcactaatac aacctttgcg ggacaacgtt ggcaggcagc
      301 aatcaacgac tgaatctcag cattaatcgc ctgttccatc gcctgataat cccattcatc
      361 agggtcttta tcgccgccat aatcaaacaa cagattagtc accatggtgg taacgcattc
      421 cagcaataca gcctcatgcg ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca
      481 gcgctccact gtgtgccagt gcgtcgggcg gccttgccga tgatgttcta tccgtgcagc
      541 catctcatca tcaaggattt gcgaggtggc gtatacagaa cctgtgaaga gtccccaata
      601 agcgcctctg cgtggcgact cttcccgctc cgtacgcccc ccgtcaccga aatcatcata
      661 ccgactcctg attcaattgt atgatggtat tcattgaaca gaacaatgga tcccccaccg
      721 ttgctgaccg caaaacattt acgcgattaa tactgcgcgt aatataatta aaaatccagc
      781 attctcaatc cattacgaaa gaagatggca ttgcggaata gccagacttc ctcgtgttaa
      841 cgtccgacaa aatgcgtcat tgacagcggt ttctctggaa tgacagattt cattcagata
      901 atggattaat gctacagccc caccagccgg accagtcagc aaacgggcat tgtgttctgc
      961 aatcaaaatt accgtcgttc cctcagaatg agcgagccat tctggtacgg atatctcgct
     1021 ttcagctgct atcaatagca gcactgaata atcgctatac gaatctgata acgattgcac
     1081 aggcccacca tcaagaggct ggaaatcttg ctgccagtcc tcaccaacaa aacaggttat
     1141 tggcaatata tcagccagat gtttcgccag atccacatct ccagataaga ggcaaaaaag
     1201 agctatcgat tcgggtttta tcgttttcat gcctctcgcc ctccacttaa tttcctgtaa
     1261 tggcgctctg gttgataagc catgaaacaa tattgccaca ccttgaatgc agccct
//
LOCUS       DADLTD010000372         1303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLTD010000372 DADLTD010000000
VERSION     DADLTD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.376"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..909
                     /gene="pocR"
                     /locus_tag="JN701_004206"
     CDS             <1..909
                     /gene="pocR"
                     /locus_tag="JN701_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HAY7366429.1"
                     /translation="SAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFTPF
                     CQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGFVL
                     CGQVRLRNDYVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENC
                     LKKQLNFVVIKENKSQSDSVRQTRAPSPHDNKMKKALRYIDAHLSDDLRLEDVASHVY
                     LSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYFCK
                     VFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(998..1174)
                     /locus_tag="JN701_004207"
     CDS             complement(998..1174)
                     /locus_tag="JN701_004207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366430.1"
                     /translation="MLVLTDWLVFWLSVILSRPSQSRDQWHFRVCQHYSSGGCEGFSL
                     SSQAQMCHTCQRML"
     regulatory      998..1173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          353 a          277 c          324 g          349 t
ORIGIN      
        1 tctgcggcga cacttaattc agaactcatt aataaaatcg cgcaggattt tgcgcaggcg
       61 accagccttg cggttgtcgt ggtcaatatt cacggcgatg aaatttccga gctgtttaac
      121 ttcactccat tttgtcagat gatgcggcaa caccctcagc taagcactcg atgtcgcatg
      181 agcgatcgtt gtgggggcct ggaagcctct aaatccgatc agccttgtat ttatcgctgt
      241 catgccggat taaccgattt ctctattcca ctggtaatcg ccgggcattt agtcggtttt
      301 gtattgtgcg ggcaagttcg tctgcgtaat gattatgtcg atttggtcga tattctgaat
      361 gtcgatgatt gctggcaggc agatcccgaa ttacttaatg aatttcgcaa agttcctgag
      421 atggattact caagagttat ggcctctgct gatttgttga agttgattgt agaaaattgc
      481 ctcaaaaaac aactcaattt tgtggttatt aaagagaaca aatctcagtc agattcggtg
      541 cgccagactc gtgcgcctag tccccacgac aataaaatga aaaaagcttt acgctatatt
      601 gatgcgcatt tatcagacga tttgcgtctg gaagatgtcg cctcgcacgt ttatctaagc
      661 ccttattatt tcagtaagtt attcaagaaa taccatggaa tcgggtttaa tgcgtgggta
      721 aaccaacaac ggatggccag cgcgcgtgag ttactgtgcc atagtgactg gagcatcgcc
      781 agcattgcgc gtaatttagg tttttcgcaa accagttatt tttgcaaagt tttccgtcag
      841 acttatcagg ttacgccgca ggtgtatcga cagcaagtga gtgaaaatgc ggcgatggaa
      901 acggtataga gtaaatgcta aaaaaaggga aggtttaaat aaacgctgga atgaataatt
      961 aacgttattt acgtgttatg ggaaaagtaa aatcagctca taacatacgc tgacaggtgt
     1021 ggcacatttg tgcctgggaa gagagtgaaa atccctcgca gcccccgctg ctgtaatgct
     1081 gacaaacccg aaaatgccac tgatcgcgag attgggaagg tcgggagagg atgacgctaa
     1141 gccagaagac cagccagtca gtcaatacca acaacttcgg tgggaagtgg gttgcccgga
     1201 agcgtccggt agagtacagg cgcgtcttta tacccctacc accctgaaag gatcagggta
     1261 tggcggcatc atttagttct ttcatcgcag tgccgacagg cac
//
LOCUS       DADLTD010000373         1274 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLTD010000373 DADLTD010000000
VERSION     DADLTD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.377"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(57..896)
                     /locus_tag="JN701_004208"
     CDS             complement(57..896)
                     /locus_tag="JN701_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7366431.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
BASE COUNT          330 a          313 c          296 g          335 t
ORIGIN      
        1 tgagggcagg tgtaaaacaa taatcaagca tggggttcat atccatagct ccatttttat
       61 tttaaggaaa gcaactgctc tacggtcatg tttttaattg cgccccggtt tacaagagtc
      121 cagccctgtt tttccagata aaaccggaaa gtctccaggg tacagaccag tgcgccatca
      181 ggaacggttt cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga
      241 ccatcacccg gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc
      301 atgcggtcga actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag
      361 cccatcacca ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg
      421 cggtgttcga atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgaag
      481 gattctattt tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata
      541 gaagtagtga cggcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtagtagcc
      601 ataatgacat cctcaagtga taagttaatt aactcaccag cagaggtgct aatctcatgg
      661 gtggtgagac gcacagggtt agcactaccg gtcactcgag aacccggcca gccttgcggc
      721 tgccccgcac gcctcaccat aatttgaatg tggctgtgca ttacgcataa aaaaaccgct
      781 tcagcgcggt tatgcgctcg agtaactttc ggggtgctaa tcccggcacc cgttttatga
      841 ggtgcaggtg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      901 tctttccttt catcaccgaa gtgaattttg tgatgcggtg cctagtgcct ccaggtgacg
      961 ttaaccagtt aacaattaca gtcggctttc ccacccaaac caataaggac taacatgact
     1021 tttaactgtg ccgcgtgcgc ttagccgcat tcaccgcatc acaaaattca ctttaaaaag
     1081 ggcggacatc agccagcaat taaaccgatg ccgccaactg gtacttcaca cagcaatgtc
     1141 gttatttaca accggaagcg cactcccacc atttaaattt cacagacaag accgactctt
     1201 tatgaatacc ggaaatgcgc cttcgtgttg tgcccggttt tatttcacca cctccgggct
     1261 ttggtggcct cggc
//
LOCUS       DADLTD010000374         1261 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLTD010000374 DADLTD010000000
VERSION     DADLTD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.378"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..1174)
                     /locus_tag="JN701_004209"
     CDS             complement(<1..1174)
                     /locus_tag="JN701_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7366432.1"
                     /translation="MVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINR
                     FGQKRAFIIGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGS
                     TSKKTGTSVNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMIL
                     RYFFIGESAMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYG
                     ILFNDRYRKRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALC
                     GVTGGMIGSFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHS
                     AGPGGLGMTIATLSYLPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWL"
BASE COUNT          372 a          355 c          280 g          254 t
ORIGIN      
        1 ccaaccagta aaacgcttca gttttcaacg caccccacag catcggccag aaaatgagtc
       61 ctgcaattgc ccctgtgcgc ataatagcgc gggcaaatcc gaccccagta gggcgaatag
      121 cgggaagata agagagtgtg gcaatggtca tgcccagccc accaggaccc gctgaatgga
      181 agaaaataat tgatcccaat aaccctaaag acagccaggg attagtcgtt gccagtgcgc
      241 ctaacgacag caatgctaag gtcacaacag caaatccata catcgactgt cggcgagtac
      301 ccagtcgttg gagaataaac gagccgatca tcccgcctgt caccccacaa agggcattca
      361 cgacggcaga accggtcaac gcgccagtaa gcccgccact tattattccc gccaacgtaa
      421 gaggaagata aacacctacg gcgttatatt gccacgcctg catggttgcc acgacacagc
      481 caagaatggt gcgtttgcgg taacgatcat taaataagat gccatatcca ccactgtatt
      541 tattctctgc tttttccgag aatttcgctt ctgttgtacc cgatgccgca acgcgagcct
      601 gaacaccata acgttttccc agaatgtcgc acgcttcctg gtaacgcccg acgcgtgcgg
      661 cccacattgc ggattcacca ataaagaagt aacgcaaaat catgaagatg actgcaaaaa
      721 tagctccgat gaatattccg taacgccaca attgttcttc agcgataccc gacagaagca
      781 gcggcaaaag taccaaataa acaacggttg tcgaaacata ccaggccatt tgccagaggt
      841 tgaccgacgt tccggttttc ttcgacgttg aaccacgaag ctctgccacg gcattggttg
      901 ccaaagggaa gtcgatgcct aaaccaaagc ccatgatgac gcggcacacc agcacccacc
      961 agatactggg agcaatagct accgctgcag caccgatggt gcacagcccc atgccaataa
     1021 taaatgcccg cttttgccca aagcggttta ttactcggtt cgctaataaa ccgccaatta
     1081 acgcggcgat taatactgat gcattgacgg ttgcagcaag acccggcgaa atgccgaatt
     1141 gagcggcaat gtatttatta ccaaacccca ccatcgccgc ctagtaggca tcaatcaata
     1201 tgccgcccaa agcaataaaa ataacgccga tactggtgtt gattgcagag ttcttattaa
     1261 c
//
LOCUS       DADLTD010000375         1242 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLTD010000375 DADLTD010000000
VERSION     DADLTD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.379"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            220..1167
                     /locus_tag="JN701_004210"
     CDS             220..1167
                     /locus_tag="JN701_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HAY7366433.1"
                     /translation="MKNVLVTVPSFSARCVYASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIALRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT          340 a          241 c          298 g          363 t
ORIGIN      
        1 aggtttagca ccgtcatgct cgtctactgt tgttctggtt gttggtgatg cggtggcgct
       61 tgcactctct gaattgaaaa aatttactcg tgctgatttc ggcttatatc atccaggagg
      121 tgcactcggc attaaagcaa attcataatt tcaaagtata aatacagtga ccggtcacgc
      181 catcttttgg gggggcgttc attgaagtga gtgtcagcta tgaaaaatgt tctggtcacc
      241 gtccccagct tttcagcacg ctgtgtttac gccagcaagt tgctaagaga gaataatttt
      301 aatcttatta ttaagaataa tgtggagcat ctgcttaaat cagaatccac tgcattacga
      361 gaatctattt gcgcggtcat cgccggaaaa gattgttatc aggcggacac gctatcattg
      421 ctgcctggtg tgaaaataat atcgagattc gggacgggga tagataatat tgctcttcgt
      481 gcagcacaac aatccggtat tgtagtcaat aatgcggttg ggattaattc aaatgctgtc
      541 gcggaattta tcattggact tatcttcgcg agcatgagaa atattcccgg cagctatcat
      601 gcgatgcaaa atggctactg gggtgagtcg catggctatg aattacaagg caaacggatt
      661 ggcatggttg gctacggcaa tataggtaaa accctggcaa aaaggttatc cggttttgat
      721 gttgagcttt tagcttatga caaacaaccc gattatcagg ttgctgacaa agttggagtt
      781 cagtttgtat caattgaaga tatctttatg cagtcgcatg tcatcattgt tcttttgcct
      841 ttttctcctg agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt
      901 gctctaatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt actacaggtg
      961 attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc gttggcgcaa
     1021 tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatattgc ggccgccacc
     1081 gttgagtcat accagcaaac cggtacacac gttgcccagt cgataattga ttacttcgca
     1141 ggaagggaga taaaaaacgt gttgtgagtt cagcgaacga agtttttaaa tacaaaaaaa
     1201 ccgcaatttg tggaacacat tgcggtttat cagcattcta ag
//
LOCUS       DADLTD010000376         1222 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLTD010000376 DADLTD010000000
VERSION     DADLTD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.380"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="JN701_004211"
     CDS             complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="JN701_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7366434.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQ"
     gene            895..1011
                     /locus_tag="JN701_004212"
     CDS             895..1011
                     /locus_tag="JN701_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7366435.1"
                     /translation="MQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            1085..1204
                     /locus_tag="JN701_004213"
                     /pseudo
     CDS             1085..1204
                     /locus_tag="JN701_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          349 a          220 c          303 g          350 t
ORIGIN      
        1 ttgcagagca tgggatgata atgggttatc actaatatgt attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttcta tcaaaaaaat actctctgac
      121 aacgggatta tttccctcag aaaatgctgg tagctcagtg aggaaattac gggtcgctct
      181 gagattttta agagcctgtg ggagcgatgg taaggagact atttcgttat agctgatatt
      241 catagtcaat aacgaatcgg gtaaagctgg caggttttcc agctcattgt gttgtacctg
      301 aagtgactcc agcgcaggag gtagcacggg caattctgat aatttattgc tggcggaata
      361 taattttttt agcgtaacag gcagttcagg taggttagtt aattgattat atgatacatt
      421 gagcagcgtt atctgagctg gtaagttgtc aggtagcgag gacagattta aacggtccag
      481 tcgaagttca tcggaattat tgataagaca ttctttaagt cgggagacag cctcatcacg
      541 ctcttcaccg gggagcgctt gtttttccca tttatcccat gctgaaaagt aatcagcata
      601 tgtaccggaa atagtgttat aaaaagaatt ttggggcaat gaaaagttat tatttatcgg
      661 taacatgatg ggcatccagt taaagccggt ggacaaatgg ctttgttaaa agaaataagc
      721 aggagttttt atgaagttca ttccatgaaa tgaacttcag cactttttta ttataataag
      781 gataaacaaa attggctgta aaaaaacgat actgtttcag ttaaccgtga atggcctgtt
      841 tgcaccgtat tgttcttgtt tttcggttag ttaaacatcg tacttcataa gtggatgcag
      901 tctaaataca gactttcgaa ccggaaggct cagcgaaagc tggcgctggt aaggaaacag
      961 tatccgaagc tatttgccca ctggtattta ctacgtgcat cgaatgagtg atcaagagcc
     1021 gtatgacgcg agagtgtcac gtacggttcc gtgagcagcc ggagagtgaa actctctccg
     1081 acggggttcc gatagcggtg gcgaacatgc ggcggtgttg tactcgctga tcggcacatg
     1141 ccgtctgaac aatgtggagc cagaaaaatg gctgcgttac gtcattgagc atatccagga
     1201 ctgaccggca aaccgggtac gc
//
LOCUS       DADLTD010000377         1215 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLTD010000377 DADLTD010000000
VERSION     DADLTD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.381"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(74..277)
                     /locus_tag="JN701_004214"
     CDS             complement(74..277)
                     /locus_tag="JN701_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7366436.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            523..858
                     /gene="iraM"
                     /locus_tag="JN701_004215"
     CDS             523..858
                     /gene="iraM"
                     /locus_tag="JN701_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7366437.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1139..>1215)
                     /locus_tag="JN701_004216"
     CDS             complement(1139..>1215)
                     /locus_tag="JN701_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY7366438.1"
                     /translation="ADGVLVAGVVVVAFPLLKKKTPDC"
BASE COUNT          391 a          307 c          211 g          306 t
ORIGIN      
        1 gccggtgcat ttagtcccac tgccagactg tcttttatgg cctggaatgc cgggccagta
       61 cgatgacgta gtatcactcc ggctcagttg caccactgac caccacatca ccttctgctg
      121 caatcgcctg catcagggta taaggggtta tggccaccgg actaccaaac ggctgccagc
      181 cctctttcag tttatgtgtc agcttttccg caagatctga cggcggcgcc gccctgacaa
      241 catcatagtg tttaaatgcc atggttcttt ccaccatctg aaaaataatt ctttaaaata
      301 cctgacatgt aatacagaaa aaacacaaaa ccatacctta aataaaaacc tgattatcaa
      361 gcagatatgc atggataaac tacaagacga gatataaacc accctgcatt taaataaaca
      421 ataaacaaca tcagaaaaat aattctgctc tatggtttac attcaaaaat atcatttata
      481 cttttcagaa catcaccagc aaggcataaa caaggaaact aaatgaagtg gattgtgatt
      541 gatacagtta tccagccatc atgcggaata tctttttcag tcatatggag taaaataaaa
      601 ttaataatct ggtatcaatc ggatgctttc ttacctcctg aaagtatatt tacactgact
      661 cacacaggca tcatgctcaa taacaaagtg ctacctgtaa ccatttacaa cgtagtacca
      721 ttcaataaaa cattctggaa tttaatcaaa aacagccagg aatgccctac aaatacagat
      781 aacgtattga atgaatgctt taataaccgt tgcactctgc aaatatgtcc ttatggacta
      841 aaacaacaaa gtccataagg agtttactca catctgacaa aatcaatata aacagcccct
      901 ccggagaggg gctggagagt ggcgctatgt gccattgcat ggtgccgggt gcctcccggt
      961 gaattcagta ccagcacctg aatccgcgat tatcccatat acctactcgc tgattgcccc
     1021 tccgcacagg gggattcacc atgccagttt cttttaacaa actccccgca aaccagacaa
     1081 cagtcaaccg cctgaattgt gaagtattta aaaatttctc ccgctaactg atacccggct
     1141 aacagtctgg cgttttcttt ttcagcaacg ggaaagcaac aactaccaca cccgccacca
     1201 gcacaccgtc agcca
//
LOCUS       DADLTD010000378         1210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLTD010000378 DADLTD010000000
VERSION     DADLTD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.382"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(48..1190)
                     /locus_tag="JN701_004217"
     CDS             complement(48..1190)
                     /locus_tag="JN701_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY7366439.1"
                     /translation="MSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFGDSA
                     NENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIEN
                     WALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSA
                     PNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFS
                     DASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGAH
                     SDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGE
                     SILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
BASE COUNT          347 a          296 c          220 g          347 t
ORIGIN      
        1 aaaccttttt taaatcctga catacacaac tccttaacta aaatagatta ccaccagact
       61 tccgcctgga tgccggccat aaactcgtct ttgctgttct cattaaactt aaaattcgat
      121 aattcgttat ccagaatatt gatatacgtt ccatagaagc gaatttctgg tcgtgaacct
      181 aaaatgcttt cacccacttt caatgcatgc cagagtgtcg ttttataagc cgattcattt
      241 agtgttacac cctgctgagt tttgttctgt tgcttaaacc agcctaattc aagccccgtc
      301 tgattccatg tattccagat ccaggccggt cttattacag tgcgaatact gtcaaaatca
      361 ctatgagcac cactttcata actataaaca tcttcgccgt gagaatagac aagcgcgtta
      421 gccataataa tattgtcagt aagatacatc tcgccctgag agattaaacg ccaggcgatc
      481 ccattggtat gatcaccata gtaatagcga ccatgcgcca tcgtattact ggcatctgag
      541 aaactggcaa aactgctggc ataggaatta ttggcaacct gtaacgtaaa ttcattaaac
      601 gtatcgcgtt gcaggttttg ccgtaaaaca gaagtcagca tccagctatc tttcatttca
      661 aaataactgt tgtcattttc gttatcttgt tgttgatccg ttttattagg tgcggaatat
      721 ttagccatta atgataatgt cgcatcatcc cataacggga tattgcgata acgaacatcc
      781 acagaattgg tattcatctg gctggtacgc gaaaaatcac gagagtaaac atcgacatca
      841 tcacggctta aggacatatc aaacagccct acaccaagtg cccagttttc aatccccaca
      901 cccgcggcga catccgtggt taaggttttc cagtccagca tctggatctc atattgcggg
      961 agtttatgtt taccgaccca gaagtctgcc tctggtgcga agggtaaaaa acctcgcgtt
     1021 gtcaggtaaa tatcgctgaa ctgcatgata ttttcattgg cactgtcacc aaaccaggca
     1081 tcgttatact gctcacccac gttgccatca taggtaacga ccgcatttgc cgttttaccg
     1141 tcctgattat aaacacgctg atttaaggtc aggtcaaacc aaccgctcat ctcattacca
     1201 aaacgtccca
//
LOCUS       DADLTD010000379         1199 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLTD010000379 DADLTD010000000
VERSION     DADLTD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.383"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JN701_004218"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JN701_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY7366440.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          214 c          259 g          385 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DADLTD010000380         1198 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLTD010000380 DADLTD010000000
VERSION     DADLTD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.384"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            59..1069
                     /locus_tag="JN701_004219"
     CDS             59..1069
                     /locus_tag="JN701_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7366441.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVASFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
BASE COUNT          283 a          294 c          324 g          297 t
ORIGIN      
        1 tcgatgaagg tatcgcgcaa cgctaatccc ctatggatat cgattctgga gaataacaat
       61 ggataaaatt acccaggttt tattttcaga tattgggaaa gtcaccactc aatatgttga
      121 agtaccacac caggaactta aaccgcacga agtgcggatt gcgcctgtgt tctatgggat
      181 atgcggttcg gatctgcatg ttctgaaagg cggtcatccg tttgccaaac cacctgtcgt
      241 ccccggtcat gaaattgcag cgcgcgttac ggaagttggc agcgacgtta aaaatgtaca
      301 gccgggcgat catgttgtgg tcgatcccat catggcttgc atggaatgcc gagcctgcaa
      361 agcaggacgt tttaatcttt gtgaaccacc gcaggttgct agttttcgcg caccgggctt
      421 tgctcgctca caacacattg ttcctgcgcg taattgccat gtcgcaccag cctctttacc
      481 gctaaaagtg ttggcctttg ccgaaccggc ggcttgtgcc cgtcactgcg ttaaccgaat
      541 gccgaaagct tctctggaaa gcgtactggt aattggtgcc ggaacgatag gcttatccat
      601 cgtgcaggca ctgcgcatta tgggggcagg taagattacc gtgattgaac ctgacgctgc
      661 caaacgcgcg ctggcgttaa agctgggcgc agcagaagtt tgggcaccag gtgagctggc
      721 cgcagatgtg cgatttacgg gggcgattga tgtcgttgca gcgcaggcca cgcttaacga
      781 tgcatgtacc cgtgtatatg ccggaggcac cgtcgtgtgc atgggcgtac caagtgggcc
      841 gcgtgaaata ccattaccga tgatgcaacg tttcgagcgt gacttgctca actctggcat
      901 gtacatccct gaagatttcg atgctgttat cgaatggctg gcggatgggc ggtttgatac
      961 cagtgaactg gttaccgatt tatttgccat tgaggatgca gcggcggcat ttgaacgcgc
     1021 gcagcaaaat gactccataa aggtcatgct gcaatttgcg ccggaatgat tttctaaatc
     1081 tctgtctggc gctgcgtcac gtcagcgcca ccctacaaca attaaaaagg acactgcgat
     1141 gagtcaaatc accagtcctg caacttattc aattagtcgg ccacaggacg taattgat
//
LOCUS       DADLTD010000381         1193 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLTD010000381 DADLTD010000000
VERSION     DADLTD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.385"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..77)
                     /locus_tag="JN701_004220"
     CDS             complement(<1..77)
                     /locus_tag="JN701_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7366442.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKS"
     gene            266..796
                     /gene="cybB"
                     /locus_tag="JN701_004221"
     CDS             266..796
                     /gene="cybB"
                     /locus_tag="JN701_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY7366443.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1041..>1193
                     /locus_tag="JN701_004222"
     CDS             1041..>1193
                     /locus_tag="JN701_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366444.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISAS"
BASE COUNT          319 a          258 c          270 g          346 t
ORIGIN      
        1 ttgcttttga cttcaagtaa tcgacgcaac accagtcgac cgatacgacc aaaaccgtta
       61 ataccaacct tactcatggt tttctcctgt caggaacgtt cggatgaaaa ttgatccttt
      121 ccaggcttag cccaggattg tgggatgggc aatctccatt cttacagtga aacgtaacgt
      181 actgaaaacg ggtgaacaat atttaatgaa attttgagaa aagcccgtta tgttaacgga
      241 aaattatgtt aaagcaggaa atgttatgga aaataaatac tcaaggttac aaatcagcat
      301 tcactggctg gtctttttgc tggttatcgc agcgtattgc gcaatggagt ttcggggttt
      361 cttcccacgt agcgatcggc cactcatcaa catgattcat gtttcctgcg gcatctcaat
      421 tctcgtgctg atggtcgttc gtcttttgtt aaggctgaaa tacccaaccc cgccgattat
      481 acctaagcca aaaccgatga tgacgggact ggcgcatttg ggacatttgg tgatttatct
      541 cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccggg gcaacccgtg
      601 gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgaac gggttgatag
      661 cttaaagtcg tggcatgaaa cgctggcgaa tctgggatat tttgtcatcg ggttgcacgc
      721 tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac gcatgatgcc
      781 gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg acccgtggta
      841 caggcgaaga atacgggtct acatcggaag cgcctattat atttatttgt atgacaaata
      901 aaaccataat ccttgcccat acgtccatct ggcttatttt taatcaattc acccgatctt
      961 tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcga acataagatt
     1021 cgataaacca tggaggttat atgaaaaaac tggcacttat tttgtttatg ggaacgcttg
     1081 tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac
     1141 actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgcc tcg
//
LOCUS       DADLTD010000382         1166 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLTD010000382 DADLTD010000000
VERSION     DADLTD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.386"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            26..1166
                     /locus_tag="JN701_004223"
                     /pseudo
     CDS             26..1166
                     /locus_tag="JN701_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          296 a          279 c          344 g          247 t
ORIGIN      
        1 cagacaacca gtatcactta aataagtgat agtcttaata ctagttttta gactagtcat
       61 tggagtacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac
      481 gtcattctca gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat
      541 acggatgtgc ttctccgtat acaccatgtt atcggagagc tgcccacgta tggttatcgt
      601 cgggtatggg cgctgcttcg cagacaggca gaacttgatg gtatgcctgc gatcaatgcc
      661 aaacgtgttt accggatcat gcgccagaat gcgctgttgc ttgagcgaaa acctgctgta
      721 ccgccatcga aacgggcaca tacaggcaga gtggccgtga aagaaagcaa tcagcgatgg
      781 tgctctgacg ggttcgagtt ctgctgtgat aacggagaga gactgcgtgt cacgttcgcg
      841 ctggactgct gtgatcgtga ggcactgcac tgggcggtga ctaccggcgg cttcaacagt
      901 gaaacagtac aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa cgatcttccg
      961 tcgtctccag tggagtggct gacggataat ggttcatgct accgggctaa tgaaacacgc
     1021 cagttcgccc ggatgttggg acttgaaccg aagaacacgg cggtgcggag tccggagagt
     1081 aacggaatag cagagagctt cgtgaaaacg ataaagcgtg actacatcag tatcatgccc
     1141 aaaccagacg ggttaacggc agcaaa
//
LOCUS       DADLTD010000383         1136 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLTD010000383 DADLTD010000000
VERSION     DADLTD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.387"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            14..172
                     /locus_tag="JN701_004224"
     CDS             14..172
                     /locus_tag="JN701_004224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366445.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
     gene            172..762
                     /locus_tag="JN701_004225"
     CDS             172..762
                     /locus_tag="JN701_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAY7366446.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            749..>1136
                     /locus_tag="JN701_004226"
     CDS             749..>1136
                     /locus_tag="JN701_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366447.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIR"
BASE COUNT          289 a          247 c          336 g          264 t
ORIGIN      
        1 gccagagggg cgtatgtatc tgtccagaat cagtgaggcc atcagtttgt ctgctggtga
       61 agtggcccac cggttaatcg tcccttcatc ggatattgac ctgggggaga ccgagcttcc
      121 ggtgctcggc gagatcacct ggcaggctta tgacccggca aggagtaaat aatggattcg
      181 ttacaggatg attatacaaa gctgctgtac ggactgatgc agccggggcc tgcatggtca
      241 gataccgacg gtgtacttga cggtctggca ccatcgcttg tgcgtgttca ccagcgggct
      301 gatgaactgg tgattgaaat cgatcccggt cagtccacag agctgattga acgttatgaa
      361 gaattgtatg gtttacctga ttcctgttcc cctgtcggaa cccagacatt acgccagcgt
      421 cagcaacgtc ttgaagcaaa agccaatgtg gctggtggca taaatgagca gttcttcctg
      481 gatcagcttg aggcgctggg atataccggc gtaacgatcg aacagttcca gcacctggat
      541 gcaagccccg atccggaatg gggagatcgc tggcgttatt tctggcgtgt gacgttgccg
      601 gtggatgccg gtgctcagtg gcagacatgc acggacgcct gcaacacacc gatccggacg
      661 tggggggata cggttgcgga gtgcgtgatt aataaattat gtccgtcaca caccgtcgtt
      721 ttattttcct atccagatga ggatgaagat gcacaggatt gatacgctga ctgcggtaaa
      781 agataagttt ggtccgggta agaacggatt tactgacgga aatcttcgca caggacgtct
      841 tgctacctgg ctgaacagtg ccatgtggaa tgccattcag gaggaaatct gtggtgtcat
      901 tgagaaggcc gggatagaac tgaataaaga agaacacgac cagctatata aggctatatt
      961 attgctggtg ggcggtgcaa ttaacgaaga ggcattgctg ataaaaaata acctttcgga
     1021 tgtggaagac agggatgagg ctgttgaaaa cctcggatta aaacccacgg tggataaggc
     1081 aaaaaatgcc gttcagcgtg atggcgacac tatgaccgga gagctgaaaa tccgtg
//
LOCUS       DADLTD010000384         1128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLTD010000384 DADLTD010000000
VERSION     DADLTD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.388"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>1128
                     /locus_tag="JN701_004227"
     CDS             join(<1..303,303..>1128)
                     /locus_tag="JN701_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7366448.1"
                     /translation="PEVRQRAIRMVLESQGEYDSQWAAICSIAPKIGCTPETLRVWVR
                     QHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKAEFDRLWKKLM
                     PLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYD
                     GNHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGD
                     RVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLD
                     ALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESIN
                     GLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGH"
BASE COUNT          266 a          272 c          347 g          243 t
ORIGIN      
        1 cccccgaagt ccgtcagagg gcgattcgta tggttctgga aagtcagggc gaatatgact
       61 cacagtgggc ggcaatttgt tccattgccc caaagattgg ctgtacaccg gagactctgc
      121 gtgtctgggt acgccagcat gagcgggata ccggaggcgg tgatggcggg ctcaccaccg
      181 ctgaacgtca gcgtctgaaa gagctggaac gtgaaaatcg tgaactgcgc cgcagtaacg
      241 atatccttcg ccaggcttcc gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa
      301 agtgatgcca ctgctggata agctgcgtaa gctgtacggg gtcggaccgg tatgcagtga
      361 actacatatt gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc atcctgataa
      421 acgcagtgcc cgtgcgcagc gcgatgactg gctgaagaaa gagatactgc gcgtatacga
      481 tgggaatcat caggtatacg gtgtgcgtaa agtctggcgt cagttgttac gggaaggtat
      541 cagagtggcc agatgcactg tggcacgtct catggcggtt atgggacttg ccggtgttct
      601 ccggggtaaa aaggtccgta cgaccatcag ccggaaagcc gttgccgcag gcgaccgcgt
      661 aaaccgtcag ttcgtggcag aacgacctga ccagctgtgg gtggctgatt ttacttacgt
      721 cagcacatgg cagggcttcg tctatgtggc gttcattatt gatgtgtttg ccggatacat
      781 cgtggggtgg cgggtctcat cgtccatgga aacgacattc gtgctggatg ctctggagca
      841 ggcgttatgg gcccgtcgac cgtccggcac ggtccatcac agtgataaag gttctcagta
      901 tgtatcgctg gcctacacac agcggcttaa ggaagccgga ttactggcat caacaggaag
      961 tacaggcgac tcgtatgaca acgcgatggc ggagagcatc aatggtcttt acaaagcgga
     1021 ggtaatacac cgtaagagct ggaaaaaccg tgcagaagtg gaactggcca cactcacgtg
     1081 ggtggactgg tataataatc gacgattgct ggaaaggctg ggccacat
//
LOCUS       DADLTD010000385         1102 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLTD010000385 DADLTD010000000
VERSION     DADLTD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.389"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..465
                     /locus_tag="JN701_004228"
     CDS             <1..465
                     /locus_tag="JN701_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366449.1"
                     /translation="LPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVV
                     FHQFLSDGLRPIVDEDDDAYEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQL
                     NAFFDALDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
BASE COUNT          284 a          216 c          265 g          337 t
ORIGIN      
        1 ttgcccattt cagcagcgga taagcggagg ttttattatt attttcagga aaaaaacacc
       61 ccaaacatag aacgcttctt cgtttttgat tcttccgagt atcgttatgc gttaaatatg
      121 cgtgaagtgg tatttcatca gttcctttct gatggtctgc ggccaatcgt tgacgaagac
      181 gatgatgcct acgaagacga ttattttaat gtccatatca cgctggtaaa cggcggacca
      241 gtgattccac tgagtgttga gccagatgcg ccgcaaaacg aagagacgga tgatatcggc
      301 cagttgaatg cattttttga cgcgctggat tgcgagcctg aaaccacgga tcgttttatg
      361 ataaccgacg aagatggaga agatgctttc attcgtattg gttctatcgc tatggtgcga
      421 gttgcgcttg atgtgctgga accagtggaa gacgatgatg agtagtattg aatggattga
      481 tttcttaaga aatggtactg agtatacatg tgagtataca aagtaacttc gtatttttaa
      541 aaatccattg atttcatatt gttgagtctg gaatcgcggt gcggccttcg caccacaccg
      601 tatgcaaatc gagtcgctca agagcggctt tttttgtgtc tgaaatcacc catatccgtg
      661 cagtatatag gcctgccaga aaagcagttc agtattcttc attttgtagt cgatggtccc
      721 aagtttgctc tggctggaag ctgcatcgtc cacattacgt gggcagggtg gtttcgctat
      781 gctacttgaa cgttttgacc atgtggctca ggtagctaat accaaaagac gtcattttgt
      841 ctccacgccc accttggtga acattcaata aatagtttcg gtcgagagca gttatgtagg
      901 gctagcagac gagatgcgca gacatcttgt tgaagacaat tttactgacc acctgagaac
      961 gctattctac cgaataatat ttattatctt ggcgaataat gatgattacc tgtgtaacta
     1021 tggttttatg ctgatgggac cggcagtaca atttctgtag tgtactcgca atttcaggtc
     1081 tctggcgttt attctggtgc aa
//
LOCUS       DADLTD010000386         1095 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLTD010000386 DADLTD010000000
VERSION     DADLTD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.390"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            264..938
                     /locus_tag="JN701_004229"
     CDS             264..938
                     /locus_tag="JN701_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAY7366450.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIRESSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGTR"
     gene            935..>1095
                     /gene="tnpB"
                     /locus_tag="JN701_004230"
     CDS             935..>1095
                     /gene="tnpB"
                     /locus_tag="JN701_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7366451.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMV"
BASE COUNT          284 a          286 c          263 g          262 t
ORIGIN      
        1 ttataacgtt aatggactct atcaatgtca gattctcttc gcccccgcag gattcgtgcc
       61 tcttactcta tgactctatg gagtttaagc ttgatcttat tgaaaaatct taccagctag
      121 gggcctgcgt ggcccaactg gccagagagt acgggattaa tgtaagcgcc ccatcagcga
      181 cgtaagcgcc ccatcagcaa cgtcttgtga aaattgtcct gtctggcaac aatcacgccc
      241 atctatattg atggacacga acgatgaatt cccagacaac aaaagatatt ccctgcttcc
      301 gttcttattt gcctgatgcc ctgcgtttaa gatttgaaga taaactgacc atccgggcca
      361 tcgctcagcg tctgggtctc agtcattcca caatacatac gctttttcag cgatttcttg
      421 catccggtat cgcatggcca ttgcccgatt cagtttcatt cgctcaactt gacgccatcc
      481 tttatgccaa cagaaagaag gaattaacag agcctcaaat cagagaaagc tcatggcgaa
      541 aagaacggcg aaccagctat agccgtgaat ttaaggtccg tctggctaag caggcgttac
      601 agcctggggc tgttgttgcc cggatcgcca gagaacacga tatcaataat aacctgctgt
      661 ttaaatggaa aagccagtac gaggacggct tactgagcga tgatgacata caggaatgca
      721 tgcctgtccc ggtggcactg actgatacgc cggagccgac cagaccagtt acaaatccct
      781 tctggcgtaa caagcatgat gagcgccctg agggagctcc cggaaacgtc ccacggtgcg
      841 agctgcatct taaatcaggt gtggtaaaac tgtttgaccc tctcactccg gaactgttac
      901 gggcgctaat ccgcgaaatg aaaggaggta cccgatgata acgctgccaa ccggtaccag
      961 aatctggatc atcgctggca tcacagatat gcgttgtggc ttcaatggcc tggcttcgaa
     1021 ggtgcagaac acgctgaaag atgacccgtt ctccgggcat atcttcgtct tccggggccg
     1081 cagtggcaaa atggt
//
LOCUS       DADLTD010000387         1091 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLTD010000387 DADLTD010000000
VERSION     DADLTD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.391"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..520
                     /locus_tag="JN701_004231"
     CDS             <1..520
                     /locus_tag="JN701_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HAY7366452.1"
                     /translation="PLQGRYITQDPIGLKGGWNFYQYPLNPISEIDPQGLNPLILGVV
                     ALMSCGLFSSGGVIQSGQQRRDQYGRKVNPEADKLTDEHKSGVNPGGNCSPQNLSDLQ
                     NEKNRLCNQSRACAPQMLRKEILRRYEINLACASVRKQINNQCFGGGDKAHMEEENKA
                     YKTAADCSGLIK"
     gene            517..1020
                     /locus_tag="JN701_004232"
     CDS             517..1020
                     /locus_tag="JN701_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366453.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDIIGLDELDIVYNF
                     FQKKQWNEIANNLKIKDDSYALELGITFLPEKVFCYYIPLYIYVSLFNKNDFWVFESD
                     FIQQYLCPEYRDYDDFLNFVFNFSDIQLSIIAQFMSYESDAGFFYASKACMDFWEDYS
                     PLLHKKI"
BASE COUNT          358 a          164 c          227 g          342 t
ORIGIN      
        1 cccgctgcag gggcgatata tcactcagga cccaattggg ctgaaggggg gatggaattt
       61 ttatcagtat ccgttgaatc cgatctcaga gatagaccca caagggttaa atccgttaat
      121 tcttggtgtt gtagcactga tgagttgcgg tttgttttca tcaggtggtg ttatccaaag
      181 tgggcaacaa cgcagagatc aatatggacg taaagttaat ccagaagccg acaaattaac
      241 agatgaacat aagtcaggtg taaatcctgg aggtaactgt agtccccaga acctgagtga
      301 cttacaaaat gaaaaaaaca gattatgtaa ccagtctaga gcttgcgctc ctcagatgct
      361 gagaaaagaa atattgagac ggtacgagat aaatttggcg tgcgcttcgg tcagaaagca
      421 gattaataat cagtgctttg gtggtggaga taaggcccat atggaagaag agaataaagc
      481 atataaaaca gcagctgatt gcagtggctt gataaaatga aaaaaataat tttctctaac
      541 gagagtgcag tctatgatga acttatgatt catttcccat gccagccatt acctcatatt
      601 tcaaatgata ttatagggct ggatgaactg gatattgtat ataatttttt ccaaaagaaa
      661 caatggaatg aaatagccaa taatttaaaa ataaaggatg actcttatgc tttagagtta
      721 ggaattacat tcctccctga gaaagtattc tgctattaca tccctctgta tatttatgtc
      781 tcgttgttta ataagaatga tttttgggtt tttgaatccg attttattca acaatattta
      841 tgtcctgagt atcgggatta cgatgatttt ttgaattttg tttttaattt ttcagatata
      901 caactatcta ttattgcaca gtttatgtct tatgaatcag acgctggatt cttttatgca
      961 agtaaagctt gtatggattt ttgggaagat tattcacctc tattacataa gaaaatttaa
     1021 tcgtaaaagg tataaatggt ttaataaaaa acttgtctac tcgttggagt agatgaaaaa
     1081 taagatgacg t
//
LOCUS       DADLTD010000388         1085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLTD010000388 DADLTD010000000
VERSION     DADLTD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.392"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..772)
                     /locus_tag="JN701_004233"
     CDS             complement(<1..772)
                     /locus_tag="JN701_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7366454.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT          314 a          154 c          252 g          365 t
ORIGIN      
        1 catgggatga caatgggtta tcactaatat ctattgaaca ttcattcctc agattaagaa
       61 tgctttccgg gatatgactt atctgattta tgtcaaaata accgttcctt aaattttccg
      121 gcaggtatgg aatatcggtt aacatattgt cgattgccca tatggagtga atgttgtgag
      181 gtagaggggg gagttcacta agaagattcc ctattgcgct aatctcttgt aaagaaaatg
      241 gtaaaggtgg taaaactgcc agcccattac atgatacatc taatgtttcc agtagttctg
      301 gtaaaacagg aagagaacat aattgattat ttgagacatg aagctccttc aaagatatgg
      361 ggagtgtggg tagtgtgatt agttgattgt gggacgcatt caataattta agtccttgag
      421 gcaaagcagg cagttcaata agtctgttat agctgacatt aagctgtgta agggacgcag
      481 gcaatgggga gattaagctt aaattatttt tacttatatt aattgattta attcccgggg
      541 ggatttcagg taatgttgtc aggcctaatt cagacaagtc taggttcgtc tcttggtttt
      601 gtagacatga tactagtcgc tgaaaagcga tgtctcgttg ttcttcttgt atgcggttat
      661 ttttccattc agtccaatgg gttagataat tttcatatgc gcggctagtg tcgattgaat
      721 aagtggagaa cgaatttgaa attaatctat gattgttatt tgtcgggagc ataaatatca
      781 gggcgtattc tttatgttga gaggtgttgg attctttttt atctgctttg caatgttatt
      841 ggttcccttc agcagtagac aggaacttct ggaggaggcg gatatatagt ttgtgtaagc
      901 atattaactc catatgttat atattgaaga acttctgctt tacactatcc taaactggat
      961 cggtttaatg taaaaaaacg atactttttg ggggaggggg caatttttcc ttaaggtatg
     1021 aaaaaaggag ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta
     1081 tataa
//
LOCUS       DADLTD010000389         1050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLTD010000389 DADLTD010000000
VERSION     DADLTD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.393"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            264..1050
                     /locus_tag="JN701_004234"
                     /pseudo
     CDS             264..1050
                     /locus_tag="JN701_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          309 a          240 c          246 g          255 t
ORIGIN      
        1 acccttttgc ttttatatct gcgtctaaaa taaaaatgag ccgcaggtta accgcaaaag
       61 ttacatgctc acatagcaaa aagaatagcc aacttcatta tggcttcagt gagatgtatg
      121 gtcgtaggat ttcatacatt gacactggtt atacatacag taaaaatact ctctactgga
      181 gggcattttt tatggcagta cgaaaactca ccactgggaa atggctttgc gaatgttacc
      241 ccgccggacg aagtgggcgt cgtgtgcgta aacaattcgc caccaaaggc gaagcactgg
      301 cttttgagcg ccacacgatg gaagaaaccg aagcaaagcc ctggctgggt gaatcagtgg
      361 atcgtcgaac actgaaagac gtggttgagc tatggttcaa actacatggt aaatctctga
      421 cagctgggca gcatgtctat gacaaattgc tgttgatggt tgacgctctg ggcaatcctc
      481 ttgcaaccga tctcacctct aaaatgtttg cccactatcg agataaacgc ctgacaggcg
      541 ggatctactt cagcgagaaa tggaagaaag gagcaagccc ggtcaccatt aacctggagc
      601 aaagctatct aagtagtgtt tttagcgaac tatcccgtct gggcgaatgg tcgtatccga
      661 acccactgga gaacatgcga aaattcacca tcgcagaaaa agagatggca tggcttaccc
      721 atgagcagat tgttgaattg ctggctgatt gcaaacgtca ggacccaatt ctggcactgg
      781 tagttaagat atgcttaagc acaggcgcac gctagcgtga agccgtaaat cttacccgct
      841 cacaggtgac caaataccga attacctttg tcagaacgaa ggggaagaaa aacagaagca
      901 tccctatcag taaagagctt tacgaagaga tcatggcgct tgatgggttc aatttcttca
      961 cagactgcta ttttcaattt ttatccgtga tggaaaaaac gtctatcgtg ctccctcgcg
     1021 gtcaactcac acacgttctg cgccatacgt
//
LOCUS       DADLTD010000390         1050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLTD010000390 DADLTD010000000
VERSION     DADLTD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.394"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            66..1050
                     /locus_tag="JN701_004235"
                     /pseudo
     CDS             66..1050
                     /locus_tag="JN701_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          251 a          280 c          274 g          245 t
ORIGIN      
        1 gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag
       61 agcttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga
      121 gtagccgggc gattgccaga gaactggggc tctcccgcaa taccgttaaa cgttatttgc
      181 aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg
      241 aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa
      301 cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg
      361 cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg
      421 aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc
      481 acgtgttcgt tgctgttccc ggatacagcc gaatgttgta catcgaattc actgacaata
      541 tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc
      601 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
      661 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      721 cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg atggaacagt
      781 ttttacatcc cactaatgac tcgcctgcgc ccgatgggga gcactgtcga tgttgaaaca
      841 gccaaccgcc acggtctgcg ctggctgcac gatgtcgcta accaacgaaa gcatgaaaca
      901 atccaggccc gtccctgtga tcgctggctc gaagagcagc agtccatgct ggcactgcct
      961 ccggagaaaa aagagtatga cgtgcatcct ggtgaaaatc tggtgagctt cgacaacccc
     1021 gttactttat tcgtacccct tataatgggg
//
LOCUS       DADLTD010000391         1019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLTD010000391 DADLTD010000000
VERSION     DADLTD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.395"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="JN701_004236"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="JN701_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY7366455.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          290 a          163 c          249 g          317 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtgtgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg aatagcttgc ttgcaccgta ttgttcttgt ttttcggtta
      961 gttaaacatc gtacttcata tttgaacgtt ctgccggaat gcattatcaa tagaggtaa
//
LOCUS       DADLTD010000392         1007 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLTD010000392 DADLTD010000000
VERSION     DADLTD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.396"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>1007
                     /locus_tag="JN701_004237"
     CDS             <1..>1007
                     /locus_tag="JN701_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAY7366456.1"
                     /translation="VEERQRGLTSLCQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE
                     LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA
                     YPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYE
                     G"
BASE COUNT          244 a          222 c          267 g          274 t
ORIGIN      
        1 ggttgaagag cgtcagcgcg ggttaaccag tttgtgccaa attaaccgca agacccggga
       61 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
      121 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
      181 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt
      241 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt
      301 tccggtttcg ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt
      361 gtatggctat ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
      421 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg
      481 gcaacaatgg tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgattatct
      541 tgatgcctgc gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat
      601 gggcgggagt gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt
      661 ccacggcgtt atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc
      721 aattcctctt accactggtg agtttgaaga gtggggtaac ccgcaggatc cgcaatatta
      781 cgagtacatg aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccgcattt
      841 actggtaacg accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt
      901 cgctaaattg cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga
      961 ctcaggccat ggcggtaaat ctggccgctt taaatcgtac gaaggcg
//
LOCUS       DADLTD010000393         1005 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLTD010000393 DADLTD010000000
VERSION     DADLTD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.397"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..389
                     /locus_tag="JN701_004238"
     CDS             <1..389
                     /locus_tag="JN701_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HAY7366457.1"
                     /translation="FHSQQLNLSFHEKINKYGYNSPIRIIKECIENGINSTPSTGYQP
                     LILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYAIGWSATIHF
                     VAQDHFGLDVTDIKNKTYSKYRFFRI"
     gene            473..964
                     /locus_tag="JN701_004239"
     CDS             473..964
                     /locus_tag="JN701_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HAY7366458.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHRPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVTNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
BASE COUNT          366 a          175 c          147 g          317 t
ORIGIN      
        1 attttcatag ccaacaatta aatttatctt ttcatgaaaa aataaataaa tatggttata
       61 atagtccaat acgaataatc aaagaatgta tcgaaaatgg cattaattca actccctcga
      121 caggatacca acctcttatc ctgcaaagca ttaaaaccaa attactttca tcaagactaa
      181 ataaatttaa tgacttcgag gacagtttta atggcttagg aatcagtgtt catgatattt
      241 cagctcaaaa aatatctctt ttaagttttc aaaaatacgc tataggatgg agtgcaacaa
      301 ttcattttgt agcacaggac cacttcggtc ttgatgtaac tgatatcaaa aacaagactt
      361 acagcaaata tagatttttt cgcatatagt tctttttaca acggcataaa gactttgctt
      421 tcaaaccttt tttcaccaaa tttaacacta tcgaaagaat cgaaaattat ctatgaaaag
      481 caaactccag aaaattatat tatgtttatt tcttctatgc tgcatctata atttatggac
      541 attgcgtcct gtacagattt tgtatacata ctctgatgct ggcaattcag tctttctggt
      601 cgttgatcat cgaccatgga ctgacagcga taaaataaat tggtatctga agcatcagaa
      661 tgaaattaaa aatcaacatc ccctaccaga aggtagttgg catacctggt atgtcattga
      721 tatcggtaat ggcttcactg actataaaaa atatatcgaa gggccgtatg aagatctcta
      781 ctgtttcccg acaattaaga gcaatgataa ttgcatcgtg acaaattact taatggttat
      841 taatgaatac ccttatcgta acactcatat tggtattaac gattttactg agtatcaatt
      901 aacacaagaa aataaaatag agcgagtttt caatccgcat gactttaaaa aagacaattt
      961 ttagaaataa gccggataaa aaaatagctt tatccggcac tcata
//
LOCUS       DADLTD010000394          999 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLTD010000394 DADLTD010000000
VERSION     DADLTD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.398"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..157
                     /locus_tag="JN701_004240"
     CDS             <1..157
                     /locus_tag="JN701_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366459.1"
                     /translation="ILQLCRNDSLMIGRESFSVDRITPDDGGCSYIRLRREGAGNVRA
                     GGYYEGA"
     gene            144..731
                     /locus_tag="JN701_004241"
     CDS             144..731
                     /locus_tag="JN701_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366460.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            728..>999
                     /locus_tag="JN701_004242"
     CDS             728..>999
                     /locus_tag="JN701_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366461.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPA"
BASE COUNT          225 a          234 c          324 g          216 t
ORIGIN      
        1 tattttacag ctgtgtcgca atgattcgct gatgattggc cgggagtctt tcagcgtgga
       61 tcgtattact cctgatgacg gcggatgtag ttatatccgg ttgcggcgtg aaggagcagg
      121 aaacgtaagg gcagggggat attatgaggg ggcttgagaa tgccatccgc aatctgaaca
      181 gtcttgatac ccgtatggtg ccgcaggcca gcgcatgggc agtaaaccgg gttgctgcaa
      241 aagttgtctc ggttgccacc cggcaggtcg ccaggaatac cgttgcggga gataaccagg
      301 tgaagggggg ccccctgaaa ctggtgcgtc agcgcgtccg ggtatttaaa gccagtccgg
      361 caggaaaaat gtatgcccgt atccgtgtta accggggcaa tctgcccgcc attaagcttg
      421 gcacaacatg ccggcgtgcc ggtgacggac tgaaggtggg gaaatacttt tttcgtggtg
      481 cgtttatcca gcagctggcg aacggtcgct ggcatgttct caggcgtctt cctgaagcgt
      541 gttttgaaac aggaaaggac agtaaggggc ggcccagaaa aaatcgtctg cccgtggagg
      601 ttgtgaaaat tccgctggcc ggaccgctga cacaggcatt tgaggatgcc cgcgaccgca
      661 tcattgccgc ggaaatgccg aaacagctgg ggtatgccct gaaacagcaa ctgaggatac
      721 atttgggatg aacaggcaca cacaaatccg ccaggcagta ctggcaagcc tgaagggcga
      781 atgcgggggg aatgtcgtgc tttttgacgg gcttccggca tttattgatg cgcaggaact
      841 gcccgccgtg gcggtgtggc tgagtgatgc gcagtacacc ggaaaaatga cggatgagga
      901 cgactggcag gctgttctgc atattgccgt tttcgtccgt gcacaggcgc cggattctga
      961 gctggatacg tggatggaga acaccatatt tccggccat
//
LOCUS       DADLTD010000395          974 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLTD010000395 DADLTD010000000
VERSION     DADLTD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.399"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            1..554
                     /locus_tag="JN701_004243"
                     /pseudo
     CDS             1..554
                     /locus_tag="JN701_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          284 a          219 c          210 g          261 t
ORIGIN      
        1 tcaggtctgg gtcgcagatt cagttgtcca ggccttccga aatcaaccga ctgaaggcgc
       61 tggtcgcgaa actgcagcgt atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa
      121 ccgaacggcg gatacaggaa gcacaggagc gaatcagcgc acttcaggag gaaatggcgg
      181 aaacactggg tgagcaatat gacccggtac tgccatcttc cctgcgccag tcttcagccc
      241 gtaaaccgtt accagcctca cttccccgtg ccccccgggt catccggccg gaagaggaat
      301 gctgtccggc ctgtggtggt gaactcagtc cactgggatg tgatgtgtca gagcagctgg
      361 agcttatcag cagcgccttt aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc
      421 ggtgcgacca tatcgtgcag gcaccggtgc cttcaaaacc cattgcacgc agttatgccg
      481 gagcggggct tctggcccat gttgttaccg gaaagtatgc agaccatctg ccattatacc
      541 gccaaagtga tctactattc cacacagcta tttagctctc tttttgttga gctcccatga
      601 ttctttcatt tattgttact aattatttta tgttatcatg atgttagaac acgctctgct
      661 ctgtgtctct attttatcta tcatattctg aggattaact acataacaag gcgcaagata
      721 tatatccaaa aacaaataat accgttgcaa taattccttt cagtaaagga ggtgtgatta
      781 atttcagggg atttcagagt gaaaattgta caacatattg aaatatgttc atttcgttat
      841 aatacacaca agaaaatatt aagttagata atatattcta aataaccata gataaaaatg
      901 cacataatgg ggaatatttt atgaaaatac ctgaagcagt aaatcatatt aatgttcaga
      961 ataatattga tctt
//
LOCUS       DADLTD010000396          941 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLTD010000396 DADLTD010000000
VERSION     DADLTD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.400"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            5..565
                     /locus_tag="JN701_004244"
     CDS             5..565
                     /locus_tag="JN701_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7366462.1"
                     /translation="MFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAANHSKQQPSK
                     PCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQHYWNLRIVA
                     SSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQLCGYSSTSY
                     FISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(611..687)
                     /locus_tag="JN701_004245"
     tRNA            complement(611..687)
                     /locus_tag="JN701_004245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Arg,seq:tct)
BASE COUNT          259 a          227 c          217 g          238 t
ORIGIN      
        1 tctgttgttt ttaaataaga acttaacctg tgtaaagccc tggtcgcggc tggcaacccc
       61 ggttatcgct tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa
      121 gcagcaaccc agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt
      181 gtctaacttt cttgagcaat cgcggtttat tgccctactg atgtatatct tacgcagcag
      241 cgtccgcgac agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg
      301 aattgtcgcc agttcgctat gtttaagccc cagcctgctc aaaaagaaat taaaaaacga
      361 aaataccagc tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt
      421 attgatggat aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc
      481 gtactttatc tctgttttta aggcgtttta cggcctgaca ccgttgaatt atctcgccaa
      541 acagcgacaa aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat
      601 gaagaagaaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt
      661 tgctctatcc aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat
      721 tatacggtca acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga
      781 ccgcgtctga ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg
      841 tctgaatcaa gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa
      901 aattgtccgc tcctatgaga ctggtaacta tgaaaccaac g
//
LOCUS       DADLTD010000397          926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.401, whole genome shotgun sequence.
ACCESSION   DADLTD010000397 DADLTD010000000
VERSION     DADLTD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.401"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..602)
                     /locus_tag="JN701_004246"
     CDS             complement(<1..602)
                     /locus_tag="JN701_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366463.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLP"
BASE COUNT          274 a          146 c          165 g          341 t
ORIGIN      
        1 gggagttttt cttgatgatt ggaaatttca accccaaaaa aaacaaaatc gtggttagag
       61 atttctttaa tatcctcaga gtatgtattc ttatgtgttt gtatattttc agactgaatt
      121 ttatcaaagg atttaatatt aagaatatta tttgacagat tagagttact ttgatgtctg
      181 aagttaaaat tagaattaat aataatagaa agtaagtgac gttcgtctga agaaagagga
      241 tgaacttttt gtattatctc agagatatag tgcctggaca ccttatcgag atgctcaagt
      301 gaccgaaatg atgaccgtgc tccagatggc gcttcaatag aaagatttag tgatttgaaa
      361 ctggtccctt gagaaaacat tttgctataa ctttgcgctg ctatagactt ccttaaaaag
      421 tttttcacag tattagcaca gtgatctaaa tgtttttcac ttatgccaga agaagatgag
      481 tttgttacgc gtaatatatt tttttgtaat gcttttgttg ctttattcgg attcgttttt
      541 ccatcaacaa gatcaatatt attctgaaca ttaatatgat ttactgcttc aggtattttc
      601 atcccccccc caattatgtg cgtttttatc tgtggttatt tagataacat tatctaactg
      661 gacactttct tgtatgtatt atagctaaat gaacagattt taatatgttg tatggttttc
      721 actctgaaat cccctgaatt taatcacgtt tcctttactg aaggcaattt taaccgtagt
      781 atcaattatt aacccttggg cttgatatcc ctctcctctt ctgggcacgg gggatgtcag
      841 attacttatt acagaaacca aaagaattgt aagcgtcatc tcagtgtcgt ctatttcaat
      901 gtctgatgat tggcgatgat agagtc
//
LOCUS       DADLTD010000398          921 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.402, whole genome shotgun sequence.
ACCESSION   DADLTD010000398 DADLTD010000000
VERSION     DADLTD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.402"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..>921)
                     /locus_tag="JN701_004247"
     CDS             complement(<1..>921)
                     /locus_tag="JN701_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAY7366464.1"
                     /translation="RFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSL
                     LVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFIST
                     KMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDT
                     AYCIKYAILAFLALLPFLAVAGYIIFEQILNAYDSSVYANDDIENLQQFMEMQRKMII
                     AQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISG
                     ITGGLAYPLLKIWMIDWQAKNTYLLGDLDDL"
BASE COUNT          354 a          207 c          141 g          219 t
ORIGIN      
        1 aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa tcatccatat
       61 tttcagcagt ggataagcca gaccgcccgt aatcccggat atcactacca acgcacacat
      121 gcgataaagc ataccgtggt acgttaaagt tgagcgaaaa cgaatacgcc catcattcag
      181 tgacaggtta ctcataaaat gatttcgcaa cgacaccgtt aaataactgg tgctaacagc
      241 aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt ccataaattg
      301 ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat ttaatatttg
      361 ttcgaagata atataaccag caacagcgag aaaaggtaat aaagctaaaa atgcgaggat
      421 ggcatatttt atacaatacg cagtatctaa tttcaccttg aaacgatgta taccgaaact
      481 ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa aaataccaat
      541 ggaaacaata ccaactattg ccatcagaac cacagatata ataacactac ttgaactatt
      601 ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg ccattaaaat
      661 cggcaagaaa aaggttaccc accagaaccc tttcatagag caattaaaac taaatcttac
      721 gccgttgaga ctagtcatca aggcctgata acgcagtcct tttgctgcca taaatataag
      781 caaaaccaac agtgacaaag tcaaaacaca gccaacaagc ggcatatctg ctgacactgt
      841 cattaagatt gcgaaataga aaaaaacaaa aacaagacaa ctaaaaaaaa cattcccacc
      901 agtaattcca taagaaaatc g
//
LOCUS       DADLTD010000399          917 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.403, whole genome shotgun sequence.
ACCESSION   DADLTD010000399 DADLTD010000000
VERSION     DADLTD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.403"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..625
                     /locus_tag="JN701_004248"
     CDS             <1..625
                     /locus_tag="JN701_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY7366465.1"
                     /translation="FFGIHGALALTALDNGIMTLWALENIATYQQYGSVEAALAAGKT
                     FHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEPILF
                     GLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNTNGS
                     VAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            676..>917
                     /gene="chbA"
                     /locus_tag="JN701_004249"
     CDS             676..>917
                     /gene="chbA"
                     /locus_tag="JN701_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY7366466.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGK"
BASE COUNT          221 a          224 c          253 g          219 t
ORIGIN      
        1 gttcttcggt attcatggcg cgctggcgct gaccgcactg gacaacggca ttatgacgct
       61 gtgggcgctg gaaaatatcg cgacctatca gcaatatggt tccgtcgaag cggcgctggc
      121 agccggtaag accttccata tctgggccaa gccgatgctg gactccttta ttttccttgg
      181 gggcagtggt gcgactttag gcctgatcct ggctatcttt atcgcctctc gccgtgctga
      241 ttatcgtcag gtggcaaaac tggcgctgcc gtccggcatc ttccagatta acgaaccgat
      301 tctgtttggt ctgccaatta tcatgaaccc ggtgatgttt atcccgtttg tactggtaca
      361 accgattctg gcggcaatca ccctcgcagc gtactacatg ggcattattc ctccggtgac
      421 caatattgca ccgtggacca tgccaaccgg tctgggagcc ttctttaaca ccaacggtag
      481 cgtcgctgca ttgctggtcg cactcttcaa ccttggtatc gcaacgttaa tttatctgcc
      541 ctttgttgtg gtggctaaca aagcgcaaaa cgcgattgat aaagaagaga gcgaagaaga
      601 tatcgctaac gccctgaaat tttaatcgat gccggtacgg ttaatcgtgc cggtaagaag
      661 agagaggaac gatgtatgat ggatctcgat aacattcccg atacgcaaac agaagctgaa
      721 gagctggaag aagtggtgat ggggcttatc atcaactccg gacaagcgcg cagcctggcg
      781 tatgcggcac tgaaacaggc gaagcagggc gattttgccg cagcaaaagc catgatggat
      841 cagtcacgga tggcattgaa tgaagcgcat ctggtacaga cgaaactgat tgaaggcgat
      901 gcgggcgaag gtaagat
//
LOCUS       DADLTD010000400          914 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.404, whole genome shotgun sequence.
ACCESSION   DADLTD010000400 DADLTD010000000
VERSION     DADLTD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.404"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /locus_tag="JN701_004250"
     CDS             complement(<1..186)
                     /locus_tag="JN701_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7366467.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIRE"
     gene            complement(479..553)
                     /locus_tag="JN701_004251"
     tRNA            complement(479..553)
                     /locus_tag="JN701_004251"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Gly,seq:tcc)
     gene            complement(557..632)
                     /locus_tag="JN701_004252"
     tRNA            complement(557..632)
                     /locus_tag="JN701_004252"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(597..599),aa:Thr,seq:tgt)
     gene            complement(635..711)
                     /locus_tag="JN701_004253"
     tRNA            complement(635..711)
                     /locus_tag="JN701_004253"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Arg,seq:tct)
     gene            complement(713..789)
                     /locus_tag="JN701_004254"
     tRNA            complement(713..789)
                     /locus_tag="JN701_004254"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(753..755),aa:Ile,seq:cat)
BASE COUNT          267 a          234 c          211 g          202 t
ORIGIN      
        1 ttctcttatt ttgaaataca gattcgtcag ataagtcaga agccccagaa gcagacttcc
       61 cagcacacca atcgcagccc actgtgatgg actgacctga tccaaccact gcaaaaacca
      121 gtagccggca ctgccggcgg aggtgccgta ggcaatgccc gttgaaattt tgtccatgga
      181 tttcatagcc tcacctccgc aaataacgga tggcgtagtt ttacactgag aaatgaaagg
      241 gatttgaaaa gaaaaaaacg caaaagcggg cgaaacgata tatacagtaa ggaaagcact
      301 ctatccaaca aaccacccac agttaatcgg aataaaagca gagtgcttat gaatgatcgc
      361 acgcccgaag gttagtattt ctgcacagca attttgcaaa aaaaggcgat cattcataac
      421 ttaaacgtct ttcagtcact ccgggatttc ccatcatcgc agactgaaag actctgactg
      481 gagcgggcag cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt
      541 atacgatgcc cgcatatggt gccgactacc ggaatcgaac tggtgaccta ctgattacaa
      601 gtcagttgct ctgcctgctg agctaagtcg gcgctggccc accaccgagg actcgaacct
      661 cgcaccgtca acttagaagg ttgatgctct atccggatga gctagtggtg gttggtggcc
      721 cttgctggac ttgaaccagc gacctggcga ttatgagtcg ctcgctctga ccaactgagc
      781 taaagggccg gagacagaat aataaccata tgtcatcaca tctgcaaact catctgacca
      841 ccagcacgtt taacgtcctg tgccgttttt caggtataaa aaaacccgca taaagcgggc
      901 tctttcaaat gtcc
//
LOCUS       DADLTD010000401          909 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.405, whole genome shotgun sequence.
ACCESSION   DADLTD010000401 DADLTD010000000
VERSION     DADLTD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.405"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..>909)
                     /locus_tag="JN701_004255"
     CDS             complement(<1..>909)
                     /locus_tag="JN701_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7366468.1"
                     /translation="AFLTGIAGALQTPTLSIFLADELKARPIMVGFFFTGSAIMGILV
                     SQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQ
                     MFALAREHADRTGRETVMFSTFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAF
                     VVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINM
                     PLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYA
                     SVLMATTPAVELELQILNAIFLGILCG"
BASE COUNT          280 a          214 c          213 g          202 t
ORIGIN      
        1 ccacagagaa taccaaggaa gatggcattt agaatttgca attccagctc aaccgccgga
       61 gtcgtcgcca tgagtacgct ggcgtaaaaa cacataccac tcacgatagc aatgagcatt
      121 aatagtcgct tgccaatacg tttcatgtaa tagcctgcga ttaacatcat cggaatttcc
      181 agaccggcag cgataccaat catttctcca gccagtttat cggttagatg cagttcatca
      241 ataataaata gcggcatatt tatcatgtag agattattcg ccgcccacat cattgaacag
      301 accacaaaaa gtagccgcgt atcccgcttc ctatgggtgg agggtaaaat ttctacgggt
      361 tgggtaacga caggaatatt tctttgtatt gatggcaaaa acaaccagac tatcagcccg
      421 caaacaacaa atgcgatggc agcggtgaga tacatcactt taaaactaaa ccccattgcc
      481 agttcataag cgagcggtgg cccgataacc caggcaagcg agatctgagc acgtaaaaat
      541 gtactgaaca tgaccgtctc acggcctgtt ctgtcggcgt gttcacgggc gagggcgaac
      601 atttgcgggt ttgcggtaga agcaaaacta ctcagaagta cgcccgtaga gaggagaatg
      661 aagtagttgc gattccacgc aaaaagcgtg caggccagca ctccaaataa gcaacatagc
      721 agaatcagta atttacggtc gccttgttta tcggagtgcc ttgccagaaa ttgactgacc
      781 agaattccca taatagcgct accggtgaag aaaaaaccta ccattatagg acgggctttc
      841 agttcatctg cgaggaatat acttagggta ggagtctgaa gagcgcccgc aatacccgtc
      901 agaaaggcg
//
LOCUS       DADLTD010000402          839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.406, whole genome shotgun sequence.
ACCESSION   DADLTD010000402 DADLTD010000000
VERSION     DADLTD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.406"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>839
                     /locus_tag="JN701_004256"
     CDS             <1..>839
                     /locus_tag="JN701_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7366469.1"
                     /translation="LLVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQ
                     CIGVEILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFH
                     VGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNV
                     KLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIE
                     LVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPI
                     MPL"
BASE COUNT          193 a          215 c          216 g          215 t
ORIGIN      
        1 tttactggtg ggctacatcg cctggctggc gaccgcttac cggatgagct tttcccgcga
       61 aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt tgcttaatgc
      121 gcgcagccag caatgtattg gtgtagagat tttgctgcgc tggaacaatc cgcgtcaggg
      181 ccggatctca ccggatgtgt ttattcctat cgcggaagaa catcatttaa ttgtgccact
      241 gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga tgagtagtca
      301 gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc tgataaaaga
      361 tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg aaatcaccga
      421 acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctacatc gtaaaaacgt
      481 caaactggcg attgatgact tcggcaccgg caacagttca ttttcctggc ttgaaacatt
      541 acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt ctgacgcggt
      601 taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata ttgaactggt
      661 ggcggagggc gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg gcgtgcatat
      721 tttgcaaggg tatttgtacg cacagccgat gccgctacgc gattttccca aatggctggc
      781 gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta tgccgttac
//
LOCUS       DADLTD010000403          825 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.407, whole genome shotgun sequence.
ACCESSION   DADLTD010000403 DADLTD010000000
VERSION     DADLTD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.407"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            37..>825
                     /locus_tag="JN701_004257"
     CDS             37..>825
                     /locus_tag="JN701_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7366470.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQ
                     TFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
                     ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGHCYDNAPMESFWGTL
                     KNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAF"
BASE COUNT          227 a          207 c          218 g          173 t
ORIGIN      
        1 atcgaacaat ggcgacaaca atttcccatt gaagcgatgt gtcaggtatt tggtgtatcc
       61 aggagcggtt attacaactg ggtacagcat gaaccctcag acagaaaaca aagtgatgag
      121 cggctaaaac tggagattaa ggtggcacat atccgcactc gcgaaacata tggaacccgg
      181 cggctccaga cggagctggc agagaatggc atcatcgttg gtcgtgaccg actggcacgt
      241 cttcgtaagg agctaaggct acgctgtaag cagaaacgca agttcagagc gactacgaac
      301 ccgaaccaca atctgccagt tgcgccaaat ctgctgaacc agacgttcgc tcctacagca
      361 ccaaatcagg tctgggtggc ggacctgacg tatgttgcca cacaggaggg atggttgtac
      421 ctcgctggca tcaaagatgt ttatacgtgc gaaattgtcg gctacgccat gggagagcgc
      481 atgacaaaag agctgacagg taaagccctg tttatggcgc tcaggagcca gcgcccacct
      541 gccgggctaa tccaccactc tgatcgaggt tcacagtact gcgcatacga ttaccgggtc
      601 atacaggagc agtctggtct gaaaacatca atgtcgcgta aaggtcactg ttacgacaac
      661 gctccgatgg aaagcttctg gggaacgctg aaaaatgaga gcctgagcca ctatcgtttt
      721 aataaccggg atgaagccat ctcagtaata cgggaataca ttgagatttt ctacaatcgt
      781 cagcgtcgtc actctcgtct ggggaatatc tccccggcag ccttc
//
LOCUS       DADLTD010000404          820 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.408, whole genome shotgun sequence.
ACCESSION   DADLTD010000404 DADLTD010000000
VERSION     DADLTD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.408"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            122..>820
                     /locus_tag="JN701_004258"
     CDS             122..>820
                     /locus_tag="JN701_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366471.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGAPSFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASA"
BASE COUNT          313 a          146 c          136 g          225 t
ORIGIN      
        1 ggctattgtg cgctcataca ctcaaattaa agataggttc taaataaatg agcgtttttt
       61 gatagtctat ttcattaggt aatatatatt tgtaacaaat caatcaaaat ggaataaaat
      121 catgctacca tctatttcaa tcaacaatac cagcgcaact tacccagaat ccattaatga
      181 aaataacaat gatgaagtta atggattagt acaagagctc aaaaaccttt ttaatggtaa
      241 ggaaggaata agcacctgta ttaaacatct acttgagctt ataaaaaacg ccatacgagt
      301 aaacgacgat ccttatagat ttaatattaa taattcctca gttacttata ttgatattgg
      361 ctccaatgat acagaccata ttactattgg tatcgacaac caagaaccaa tagaattacc
      421 tgcgaactat aaagacaaag aactcgtccg tactatcatt aatgacaaca tagttgagaa
      481 gactcatgat atcaataaca aggaaatgat cttcagcgca ttaaaagaaa tatatgatgg
      541 agctcctagt tttatcttcg ataagatatc acacaaactc agacatacgg taacggaatt
      601 tgatgagagc gggaaaagcg aaccaacgga cttatttacc tggtacggta aagataaaaa
      661 aggcgactct ctcgctattg taattaaaaa taaaaacgga aatgattact tatccctcgg
      721 ttactacgat caggacgact accacattca aagaggaatt cgtattaatg gtgatagtct
      781 cacccaatat tgtagtgaaa acgccaggag tgcttcagcg
//
LOCUS       DADLTD010000405          804 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.409, whole genome shotgun sequence.
ACCESSION   DADLTD010000405 DADLTD010000000
VERSION     DADLTD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.409"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>804
                     /locus_tag="JN701_004259"
     CDS             <1..>804
                     /locus_tag="JN701_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7366472.1"
                     /translation="VLMPGKVHADDIPVPVQEPGSGKTRTARLWVYVRDDRNAGSQMP
                     PAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAACMAHARRKIH
                     DVHARVPTDITTEALQRIGELYTIEAEVRGCSAEQRLAARKARAAPLMQSLYDRIQQQ
                     MKTLSRHSDTAKAFAYLLKQWKALNVYCSNGWVEIDNNIAENALRGVAVGRKNWLFAG
                     SDSGGEHAVVLYSLIGTCRLNNVEPEKWLRYVIEHLQDWPANRVRDLLP"
BASE COUNT          199 a          210 c          243 g          152 t
ORIGIN      
        1 tgtgctgatg cccggtaaag ttcatgcgga tgatatcccc gttccggtcc aggagccggg
       61 cagtggtaaa acaagaactg cccggctgtg ggtctatgtc cgtgatgacc gtaacgccgg
      121 ttcacagatg cccccggcgg tctggttcgc gtacagcccg gaccggaaag gtatccatcc
      181 acagaatcac ctggctggtt acagcggtgt gcttcaggca gatgcttacg gtggttaccg
      241 ggcgttatac gaatccggca gaataacgga agccgcgtgt atggcccatg cccggagaaa
      301 aatccacgat gtgcatgcaa gagtgccaac cgacatcacc acggaagccc tgcagcgtat
      361 cggtgaactg tataccatcg aggcagaagt ccggggatgt tcagcagaac agcgtctggc
      421 ggcaagaaaa gcaagagccg cgccactgat gcagtcactg tatgaccgga tacagcaaca
      481 gatgaaaaca ctgtcgcgtc actcggatac ggcaaaagcg ttcgcatacc tactgaaaca
      541 gtggaaggcc ctgaacgtgt actgcagtaa tggctgggtg gaaatcgaca acaacatcgc
      601 agagaacgcc ttacggggag tggccgtagg ccggaaaaac tggctcttcg cgggttccga
      661 cagcggtggc gaacatgcgg tggtgttgta ctcgctgatc ggcacatgcc gtctgaacaa
      721 tgtagagcca gaaaaatggc tgcgttacgt tattgagcat ctccaggact ggccggcaaa
      781 ccgggtacgc gatctgttgc cctg
//
LOCUS       DADLTD010000406          791 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.410, whole genome shotgun sequence.
ACCESSION   DADLTD010000406 DADLTD010000000
VERSION     DADLTD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.410"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            3..>791
                     /gene="fdrA"
                     /locus_tag="JN701_004260"
     CDS             3..>791
                     /gene="fdrA"
                     /locus_tag="JN701_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAY7366473.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALERNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIAQAGE
                     GITHAIGLGERDLSREVGGISALTALEMLSADEKSEVLAFVSKPP"
BASE COUNT          191 a          181 c          244 g          175 t
ORIGIN      
        1 ttatgatcca cgcctttatt aaaaaagggt gttttcagga ttcggtcagt ttaatgatta
       61 tttcacgaaa actcagcgaa tcagaaaatg ttgatgatgt ttccgtaatg atgggtacgc
      121 ccgccaataa agcgttatta gataccacag gtttctggca tgacgatttt aataacgcca
      181 cgccgaacga tatttgcgtg gcaattcgta gcgaagcggc ggatgcgggg atcgcgcagg
      241 cgattatgca gcagcttgaa gaggcgctaa aacaactggc gcagggggca ggcagcagcc
      301 agtccttgac gcaggtgcgt cgctgggaca gtgcctgtca gaaattatcc gatgccaatc
      361 tggcgctgat ttcagtggct ggcgagtatg cggcggagct ggcgaatcag gcgctggagc
      421 gcaacctcaa cgtgatgatg ttctccgata acgtcacgct ggaagatgaa atccaactta
      481 aaacccgcgc gcgagaaaaa ggcttgctgg tgatggggcc ggactgcggt acgtcgatga
      541 ttgccggcac gccgctggct tttgctaacg tgatgccgga aggcaacatt ggcgtcattg
      601 gcgcttccgg taccgggatt caggaactgt gttcgcagat tgcgcaggca ggggagggaa
      661 ttactcacgc gattggtctt ggcgagcgcg acctcagccg tgaagtaggc ggcatcagtg
      721 cgctgacggc gctggaaatg ctcagtgcag acgagaaaag cgaagtgctg gcatttgttt
      781 caaagccacc t
//
LOCUS       DADLTD010000407          778 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.411, whole genome shotgun sequence.
ACCESSION   DADLTD010000407 DADLTD010000000
VERSION     DADLTD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.411"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            284..>778
                     /locus_tag="JN701_004261"
     CDS             284..>778
                     /locus_tag="JN701_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY7366474.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVEL"
BASE COUNT          216 a          148 c          186 g          228 t
ORIGIN      
        1 tccaggagct gctgtagaat cttctgcctg atcatcacat tctccgttaa ttctttgtca
       61 tatcgcacat tttcgttatg ccctgggccc taagtgtatc aaaaacagca agggcgaagg
      121 caaaagtact ggtagcgcct gcttcgttgt ccccgaaaat agagaggttt cagtcctgca
      181 ttattaatga attttttgca taagtgatat caaaatccac gtactaattt gaggttacgt
      241 tttaacgtag actcattgtt catgcctaat ggagggactg acagtggaat tttccgtatt
      301 aagtaacaat ctgaaaatgc cgatgatggg atttggtgtt tttcaggtta ccgataaaaa
      361 cgtgtgcaaa cagtcagtgt tgaacgctat ccgcacaggt tatcgactca tcgacactgc
      421 agcggtatac ggtaatgaag atgccgtcgg tgaagccgtt cgtgaagcta tatctgaagg
      481 tttatgtacc cgagatgagt tatttataac gtctaaattg tgggtgcagg atatgttgaa
      541 tcaggatacg gcagcagcag gtattgaagc atcattaaaa aaatcgggac tagagtactt
      601 cgacctctat ttattgcacc aggctatgcg cgattatttc agtgcgtggc gtgcactcga
      661 agatgcctat gaagagggca aattaaaagc aattggggtt tccaatttct atcctcatgt
      721 tctggcgaac ttttgtgaaa cggtaagagt taaaccgatg gtcaaccagg tcgagttg
//
LOCUS       DADLTD010000408          755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.412, whole genome shotgun sequence.
ACCESSION   DADLTD010000408 DADLTD010000000
VERSION     DADLTD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.412"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>755
                     /locus_tag="JN701_004262"
     CDS             <1..>755
                     /locus_tag="JN701_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY7366475.1"
                     /translation="HPETEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWL
                     AGMKLGDTPLVDFTRDRLHRETLRRFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWN
                     DNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPD
                     STLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGGIRTDDERTHWYHYDSQHRLV
                     HYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRER"
BASE COUNT          170 a          258 c          217 g          110 t
ORIGIN      
        1 cacccggaga cggaagcact gctctggcag catgagacca gacatgcgta caacgcgcag
       61 ggactggcga accgctgtat accggacagc ctgcccgccg tggaatggct gacctacggc
      121 agcggctggc tggcaggtat gaagctcggc gacacaccgc tggtggattt cacccgcgac
      181 cgcctgcacc gggaaacgct gcgccgcttc ggccgttatg aactctccac cgcttacacc
      241 cctgccgggc agttacagag ccagcacctg aacagcctgc agtatgaccg cgattacacc
      301 tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg gagttacagc
      361 tacagcacca ccggcaggct gaccggcgtt cacaccaccg cagcgaacct tgatatccgc
      421 atcccgtatg ccacagaccc ggcaggtaac cgcctgcccg acccggagct gcacccggac
      481 agcaccctca gcatgtggcc ggataaccgt atcgcccgtg acgcgcacta tctttaccgg
      541 tatgaccgtc acggcaggct gacagagaaa accgacctca tcccggaagg gggtatccgc
      601 acggatgatg agcgcaccca ctggtaccat tacgacagtc agcaccggct ggtgcactac
      661 acgcggacac aatatgcaga gccgctggtc gaaagtcgct atctttacga cccgctgggc
      721 cgcagggtgg caaaacgggt gtggcggcgt gaacg
//
LOCUS       DADLTD010000409          729 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.413, whole genome shotgun sequence.
ACCESSION   DADLTD010000409 DADLTD010000000
VERSION     DADLTD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.413"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..133)
                     /locus_tag="JN701_004263"
     CDS             complement(<1..133)
                     /locus_tag="JN701_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7366476.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..509
                     /gene="tnpA"
                     /locus_tag="JN701_004264"
     CDS             141..509
                     /gene="tnpA"
                     /locus_tag="JN701_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7366477.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVLWSPGYFASS
                     CGGAPISVIRQYIEQQQTPG"
BASE COUNT          205 a          182 c          149 g          193 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcaatatcca gtctggtaaa cagcctcaaa ggcgttttgt
      421 ggagtcctgg ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat
      481 acattgaaca acagcaaaca cctggttagg tagaaaacca cgccttatat ccctgccctg
      541 aaggacgggg ttttacggcg caccggataa gcctggagat tatctacacc agcatggaca
      601 tcattgtact atgccccgta catgccccat cacatcctca acatcaaagt attagagcct
      661 tgcaaaccat tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa aatagaacta
      721 atatcaagc
//
LOCUS       DADLTD010000410          726 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.414, whole genome shotgun sequence.
ACCESSION   DADLTD010000410 DADLTD010000000
VERSION     DADLTD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.414"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            35..726
                     /locus_tag="JN701_004265"
                     /pseudo
     CDS             35..726
                     /locus_tag="JN701_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          170 a          181 c          200 g          175 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcacgtc ttcggtgaac gcactctggc cacactggag
      481 cgtcttctga gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga tggctggccg
      541 ctgtatgaat cacgcctgaa gggaaagctg cacgttatca gcaagcgtta cactcagcgc
      601 attgagcgac ataatctgaa tctgagacaa catctggcaa ggctgggacg gaagtcactg
      661 tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DADLTD010000411          719 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.415, whole genome shotgun sequence.
ACCESSION   DADLTD010000411 DADLTD010000000
VERSION     DADLTD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.415"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            240..509
                     /locus_tag="JN701_004266"
     CDS             240..509
                     /locus_tag="JN701_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366478.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            506..633
                     /locus_tag="JN701_004267"
                     /pseudo
     CDS             506..633
                     /locus_tag="JN701_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553778.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          186 a          168 c          202 g          163 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg accaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtga
//
LOCUS       DADLTD010000412          718 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.416, whole genome shotgun sequence.
ACCESSION   DADLTD010000412 DADLTD010000000
VERSION     DADLTD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.416"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..509)
                     /locus_tag="JN701_004268"
     CDS             complement(<1..509)
                     /locus_tag="JN701_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY7366479.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAA"
     gene            complement(460..>718)
                     /locus_tag="JN701_004269"
     CDS             complement(460..>718)
                     /locus_tag="JN701_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7366480.1"
                     /translation="IAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIA
                     SPQFFITGCSEHSLPGSYCALSGGPDAHVSGAMPGLFIRTS"
BASE COUNT          147 a          208 c          179 g          184 t
ORIGIN      
        1 attgctgccg ccagttcttc cagttccggc atcgacagtt tcacctgctg attatcggca
       61 tcgctccacg ccatatgtgt ttgtgctgtg acagattttg ccagcatgac taccggggac
      121 aggcggccca gtgagtcggg accagcattc cagatacgac cgttccattc aaacgtgaac
      181 ggcttcgcct cctgttctgc gcgccatgct tcaatttcct gacgtctggc ctctctggcc
      241 gcttccagca tttctggtgt cacagtgaat ggggctatct caccccattt gccgctttgc
      301 agttcctgcc agatttgctg acccgtcgga gcgacatcat cagcggtggc tgtgtagggg
      361 actgcctggt ccctgtcgtc aaaaaaaacg tcacagtcta ctgcgccact ttcggtataa
      421 cggggattaa tgattttttt aatttccacg gtgcattcct cacgatgtgc gaataaaaag
      481 cccgggcatt gcgccagaga catgtgcatc cggccccccg gacagggcgc aatatgaccc
      541 cggtaatgaa tgctctgaac atcccgtaat gaaaaattgt ggggatgcta tatacgttcc
      601 ggtgggagta ctgggcactg aaatccccac cggtcccagt cgtgagcctc tgtatgactg
      661 ccccctgaca agtctgatga ctttatcacc gtcagcttct ccctggtacg cagcaata
//
LOCUS       DADLTD010000413          710 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.417, whole genome shotgun sequence.
ACCESSION   DADLTD010000413 DADLTD010000000
VERSION     DADLTD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.417"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(118..648)
                     /locus_tag="JN701_004270"
     CDS             complement(118..648)
                     /locus_tag="JN701_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7366481.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGALNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALEKVIPFFNPHLG"
BASE COUNT          185 a          190 c          146 g          189 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc acatggcggg
       61 ctatctgacc gccagcacca agaatcagta catttttcat aatatttctc atgaaactta
      121 tcccagatgg gggttgaaaa aaggaataac tttttccagc gcctcctgaa ctgcttccgg
      181 tttgtcatac agttcataat gtgagaaatc gggtaatgaa actaactgac gatcctgcga
      241 aaccatcgcc cggccataga cttccagtcc atcacgataa gcaccgaagg caccgacttt
      301 ttcaccaacg actaccatca ccggctgagt cagtaacact tcagtaaatg cgaaggcatc
      361 ccaggccagc gttttctgtg catgtgagaa caacattttt gtcgcacctc ccggttgttg
      421 cccgcgtggt gttttatagt agtcagttgc ttcataaaca tcgcgttcgg tcagtccatg
      481 ggcttttgct gcatccaggc tggcgggaag taattcgttg atctgtaatt ctccaccacg
      541 agcctctttg gttcgttgcg acgccattgc attcaatgcg ccaatcggat cgtaattgct
      601 gaaaccttcc cgaaaaagac gcccgatatt aacacccgta atactcacta ctgctttaat
      661 tcgtttttct gtcagcgcgg cattaatggc atagtcacca cccccacaca
//
LOCUS       DADLTD010000414          686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.418, whole genome shotgun sequence.
ACCESSION   DADLTD010000414 DADLTD010000000
VERSION     DADLTD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.418"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            201..389
                     /locus_tag="JN701_004271"
     CDS             201..389
                     /locus_tag="JN701_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY7366482.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKTKLTKSECEKMLSGEKEAGVS
                     KEQKVKLINFNCEKLQSS"
     gene            509..655
                     /locus_tag="JN701_004272"
     CDS             509..655
                     /locus_tag="JN701_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY7366483.1"
                     /translation="MVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKR
                     GIIW"
BASE COUNT          235 a          128 c          162 g          161 t
ORIGIN      
        1 cccgctcatg ggaaagaatc aaaggtatgg actggccgac aatcaaagct ggtcgtaatg
       61 cactatggat tgaactggaa ggcgttgaat atgcattccc ggatccacaa acacaaaacg
      121 aaacgcaaca agcaggatac gatgagaata ttccggtgta aaaccaagtg cattttggga
      181 aaatattaat ggaggtgttc atgtataaaa ttacagccac tattgaaaag gaaggtggtt
      241 ctcctgccag ctggacaaga tactcaaaga caaaactaac gaaatcagaa tgcgaaaaaa
      301 tgctctcagg ggaaaaagaa gctggcgttt ccaaagagca gaaagtaaaa ctgataaatt
      361 ttaattgcga gaaacttcag tcctcgtgaa ttgcattgta ttcaaattaa aacttcatag
      421 ctgattatta ataatcaaca tcgggcgtca atttcagcct aacattggcg cctgccagag
      481 gtgatgcgat ggctttaatg aagagtggat ggttgaatac ggcctgatgc ttcgcactgg
      541 tctgggtgcc agacaaattg aagcataccg ccagaactgt tgggtggaag gcttccactt
      601 caaacgagta tctcctttag ggaagccaga cagtaagcga gggattatct ggtaaaacta
      661 tccgaagata aatcagttta tcaaag
//
LOCUS       DADLTD010000415          656 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.419, whole genome shotgun sequence.
ACCESSION   DADLTD010000415 DADLTD010000000
VERSION     DADLTD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.419"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            215..574
                     /locus_tag="JN701_004273"
     CDS             215..574
                     /locus_tag="JN701_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY7366484.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMEFGERTVIK
                     NPEYFSTYMQEELKALV"
BASE COUNT          211 a          127 c          131 g          187 t
ORIGIN      
        1 aactatataa ataaacataa ccatgccgtt gtgttcgccc ttcgattagc acctctggtt
       61 ttctttctta gctgaacaat ctggttatag cgtgcggact gaccgttaat tttctccatc
      121 ttactggata acacaccatg agctgctacc tttaaaacac ctgaatgtca acaggttaac
      181 tcgtgccata tcgttataat ccctttgata ttagatgcaa attaaggtca tatatagcct
      241 tatcgataac atggttaatt ttaaggataa gaatatgcct gctgtaatag ataaagccct
      301 ggatttcatt ggtgccatgg atgtatcagc gccaacacca agttcgatga atgaaagcac
      361 agcgaaggga atctttaaat atttaaaaga actgggagta cccgcgagtg ccgctgatat
      421 tacggcgcga gctgaccagg aaggctggaa ccctgggttc acggaaaaaa tggttggatg
      481 ggcaaaaaaa atggagtttg gtgaacgcac tgtaattaaa aatcctgaat acttttcaac
      541 atatatgcag gaagaactaa aagcactggt ctgagtttaa tttatatcag cataaatggg
      601 tcaggacgct tttaatcaca tataaaaagc gtcctttttc tcacaatcaa ataatc
//
LOCUS       DADLTD010000416          636 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.420, whole genome shotgun sequence.
ACCESSION   DADLTD010000416 DADLTD010000000
VERSION     DADLTD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.420"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..278
                     /locus_tag="JN701_004274"
     CDS             <1..278
                     /locus_tag="JN701_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7366485.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESAA
                     IIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
     gene            complement(523..>636)
                     /locus_tag="JN701_004275"
     CDS             complement(523..>636)
                     /locus_tag="JN701_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAY7366486.1"
                     /translation="RLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
BASE COUNT          149 a          145 c          170 g          172 t
ORIGIN      
        1 gcctgaacgt gtactgtcgt aatggccagg tggaaatcga caacaacatc ggtgaaaacg
       61 cgctgcgatc ggtggcggtt ggaagaaaaa attatctctt cttcggttct gacaacggtg
      121 gagaaagcgc ggcgattatc tacagtctgc tggtcacctg caaactgaac ggagtggagc
      181 cggaggactg gttgcgcgaa gtgatcgtga agctcaatga ctggccgtcg aaccgagtgc
      241 atgaactgct gccctggaac ttctcagctg taaaataatc cttccgctac gtacttctct
      301 gggcgcttac cactcaacgg catcgtcaaa attatcaaag cgcagaacat agatttatca
      361 tgtgttttat ggatggcttt ctccatcagg catcgttcgt cacgggaaat tggtgctatg
      421 atcggcattg atcagtccgg ttggtgattt gttttgattt ggcgattgat cagatcgcac
      481 aatccgggct gagttccctc taagtgatct aggttgtgtc ccttaattac gtaagcgttg
      541 ataaaagagt cggatgcagc ccagtttcac catcgccagg taatttctcg ccgttttgtc
      601 gtaacgcgtg gcgatgcggc gatattcttt cagccg
//
LOCUS       DADLTD010000417          623 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.421, whole genome shotgun sequence.
ACCESSION   DADLTD010000417 DADLTD010000000
VERSION     DADLTD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.421"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>623
                     /locus_tag="JN701_004276"
     CDS             <1..>623
                     /locus_tag="JN701_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY7366487.1"
                     /translation="LLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFGVSSG
                     LSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIWALFL
                     PVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTSVVMF
                     LVASAQVSTWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVG"
BASE COUNT          107 a          173 c          158 g          185 t
ORIGIN      
        1 ctactggtgc cgatgatgcg cagcgccaac tacccggtca accgggcggc ggggctgatt
       61 gcctctggcg gcattatcgc gccaattatt cccccttcca ttccgtttat tatcttcggc
      121 gtttccagcg gattatccat cagtaagctg tttatggcgg gcattgcccc tgacatgatg
      181 atgggcgcaa cgctgatgct tacctggtgg tggcaggcca gtcgccttaa tttaccccgc
      241 cagcaaaaag caacgatgca ggaaatctgg cactcttttg tctccggtat ctgggctctg
      301 tttcttccag tcatcattat tggcgggttt cgctccggtc tgtttacccc taccgaagcc
      361 ggggccgttg ctgctttcta tgcgctgttt gtcgccacag ttatttaccg tgaaatgacc
      421 ttcgccacac tttggcatgt actcattggc gcggcgaaaa ccacctcagt ggttatgttt
      481 ctggtggcct cggcacaagt ttccacctgg ctgattacca ttgctgaact gccgatgatg
      541 gtttctgatt tactgcaacc gttggtcgat tcaccgcgtc tgctgtttat cgtcattatg
      601 gtggctatcc tgattgtcgg cat
//
LOCUS       DADLTD010000418          621 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.422, whole genome shotgun sequence.
ACCESSION   DADLTD010000418 DADLTD010000000
VERSION     DADLTD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.422"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(272..>621)
                     /locus_tag="JN701_004277"
     CDS             complement(272..>621)
                     /locus_tag="JN701_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY7366488.1"
                     /translation="AHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCN
                     LYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKNPKMIDFL
                     LKNGAILGKRFEI"
BASE COUNT          182 a          126 c          109 g          204 t
ORIGIN      
        1 cccgtaaaga caggagtcaa gttcccgctc cgacataact gcgtgcaatg gattttgaag
       61 gaaccgtcag cccattcccc ggaggcaaac atgggctggc aaaagtgtag cggtattagg
      121 cacagctatt taggaaagag ggaccatctc catctcatta aatggaccac gccagacaat
      181 tcactgtcat gctgatgctg cactgtgggg acagaatcac tcatgatgac ttcgataatc
      241 gacgacatta ttatttggcc gagcttttag gtcatatctc aaatcgtttg cctaatattg
      301 ctccattttt taataaaaaa tcaatcattt ttggattttt gcttttcatt gcattgtcaa
      361 gcatagtgtc cccactattt gctttttgaa attttttgtt tacatcaacc aatccattat
      421 tgataaaata ttctagcact ttatagttgg caccatcttt actaagtaag tattcaacat
      481 catacagatt acaatatcta ttgttgttca ttttctccat ctctgcgaca tcttgttttg
      541 taaaattaaa tttagatagt aaataaagtg ctatatcagc ttttttattg cctaaagaat
      601 aagcaagggc ctgatgagcc a
//
LOCUS       DADLTD010000419          590 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.423, whole genome shotgun sequence.
ACCESSION   DADLTD010000419 DADLTD010000000
VERSION     DADLTD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.423"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            244..>590
                     /locus_tag="JN701_004278"
     CDS             244..>590
                     /locus_tag="JN701_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7366489.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNN"
BASE COUNT          170 a          110 c          122 g          188 t
ORIGIN      
        1 atcgccatac cctaaccaat tgcacttgtg aatttatttt tgcttaattt tgaattaaga
       61 aaatttaatt tgttcaaaag cccttaaagt gcacaatagg cgtcctaatg aacaatggtt
      121 gtaaattttg gtaacaaata tcattgctca ttaatatcta gcatctgctt ataacatttg
      181 tgtcatgaat agtcattctt caaaccagta gtctgactaa tggaatttta ttcaaggatt
      241 tttatgtcta aaaaattagg ttttgccctg tctggtctta tgctggcaat ggttgcaggt
      301 actgcttctg ctgatatgga tggtggtcag ttaaatatca gtggtctggt tgttgataac
      361 acctgtgaaa cccgcgttga cggcggtaat aaagatggtc tgatcctgct gcaaactgca
      421 actgttggtg aaatcgatgc tggcgtgctg aatgacaccg ttggcgctaa agccaaacct
      481 ttcagcatca ccgttgactg cagcaaagca aatccgaatc caggtagtac agctaaaatg
      541 accttcggtt ctgttttctt tggtaatagc aaaggaacgc tgaacaacga
//
LOCUS       DADLTD010000420          590 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.424, whole genome shotgun sequence.
ACCESSION   DADLTD010000420 DADLTD010000000
VERSION     DADLTD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.424"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>590
                     /locus_tag="JN701_004279"
     rRNA            <1..>590
                     /locus_tag="JN701_004279"
                     /product="16S ribosomal RNA"
BASE COUNT          139 a          135 c          192 g          124 t
ORIGIN      
        1 cacgcaggcg gtttgttaag tcagatgtga aatccccggg ctcaacctgg gaactgcatc
       61 tgatactggc aagcttgagt ctcgtagagg ggggtagaat tccaggtgta gcggtgaaat
      121 gcgtagagat ctggaggaat accggtggcg aaggcggccc cctggacgaa gactgacgct
      181 caggtgcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac
      241 gatgtcgact tggaggttgt gcccttgagg cgtggcttcc ggagctaacg cgttaagtcg
      301 accgcctggg gagtacggcc gcaaggttaa aactcaaatg aattgacggg ggcccgcaca
      361 agcggtggag catgtggttt aattcgatgc aacgcgaaga accttacctg gtcttgacat
      421 ccacggaagt tttcagagat gagaatgtgc cttcgggaac cgtgagacag gtgctgcatg
      481 gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta
      541 tcctttgttg ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga
//
LOCUS       DADLTD010000421          584 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.425, whole genome shotgun sequence.
ACCESSION   DADLTD010000421 DADLTD010000000
VERSION     DADLTD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.425"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>584
                     /gene="pdeB"
                     /locus_tag="JN701_004280"
     CDS             <1..>584
                     /gene="pdeB"
                     /locus_tag="JN701_004280"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY7366490.1"
                     /translation="LPVGLSIWLAHQQVETSFIEELDTYSSRVAIRANKVATQGKDAL
                     QELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPG
                     KISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGN
                     AHNVVITSSDEIAQGIITRLQKTPGEHIENNGII"
BASE COUNT          157 a          139 c          138 g          150 t
ORIGIN      
        1 ctgcctgtcg gcttaagcat ctggctggcc catcagcagg tagaaacatc gtttattgaa
       61 gagctggata cctattcctc ccgcgtcgct attcgggcca ataaggtggc gacacaaggg
      121 aaagatgcgc tgcaagagct ggaaagatgg caaggcgctg cctgtagcga agcccatctc
      181 atggaaatgc gtcgggtatc ttacagttat cgctatattc aggaagtggt ttatatcgat
      241 aacaacgttc cccagtgttc gtctctggag catgaaagtc cgcccgatac cttccccgag
      301 ccaggtaaaa tttcgaaaga tggttatcgt gtctggttaa catcgcataa cgatttaggc
      361 attatccgtt acatggtcgc catgggaacg gcacattatg tcgtcatgat cgaccccgct
      421 tcctttattg atgtcattcc ctatagctca tggcaaattg atgccgccat tattggcaat
      481 gcccataacg ttgtcataac cagcagcgat gaaattgctc agggaattat taccaggcta
      541 caaaaaacac ccggtgagca tatcgaaaat aatggaatca ttta
//
LOCUS       DADLTD010000422          583 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.426, whole genome shotgun sequence.
ACCESSION   DADLTD010000422 DADLTD010000000
VERSION     DADLTD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.426"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(67..261)
                     /locus_tag="JN701_004281"
                     /pseudo
     CDS             complement(67..261)
                     /locus_tag="JN701_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(349..583)
                     /locus_tag="JN701_004282"
                     /pseudo
     CDS             complement(349..583)
                     /locus_tag="JN701_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase"
BASE COUNT          145 a          141 c          141 g          156 t
ORIGIN      
        1 tttcggctca tccggcgtta tctccacagg taataattca acgccagtgt cagaagaggt
       61 tgttaccgga agacgccgcg atatgcgccc ttcgttctgc cagagcctga gccatttgaa
      121 cagcaggtta tcattgatat cgtgttcccg ggcaatacgg gcaacagagg ctcctggttg
      181 tgaagccagt ttaaccattt gaagtttaaa ctcatttgaa aatgatctgc ggggttctgc
      241 agataatgct ttctgttcca taacaggtgt ccactagttg aaaaggtggg cacctacgtt
      301 accaatacag gcttaatggc tacatacggc ggtcagttta cgcttacctg gctgctgcca
      361 ggttttacca tcatcattaa gcaaattgat gccatcccaa cgtgttaatg tatccaccaa
      421 ctgacggcgc ggatcgctct gtgtcaaacc agatgtcgct gcttgcagag taggtaaaaa
      481 aagcctcagg ttaagatcct gacgactggt ttggcgaata acatcccatg cctgatcagc
      541 agttaagcgt ggcttttgct caagcagtcg gtcgatctcc gta
//
LOCUS       DADLTD010000423          573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.427, whole genome shotgun sequence.
ACCESSION   DADLTD010000423 DADLTD010000000
VERSION     DADLTD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.427"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>573
                     /locus_tag="JN701_004283"
     CDS             <1..>573
                     /locus_tag="JN701_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HAY7366491.1"
                     /translation="RHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTEL
                     LGQKEENSWRIMLMTMLISQPALAEQVLPWLSTPAVAVLKSYQQQLTQPSNHACADLL
                     PAVLVSPPWLSKKKKSVMPVLDLTPLPLESCCTLTETAEKEIHTRHRWHAHQIDIGQK
                     EDIQNYLARLGFNRWNNGQYMKASDAVVEL"
BASE COUNT          165 a          149 c          129 g          130 t
ORIGIN      
        1 tgcgtcatat caatcatccg ttcgcattga cactgcttat acgtgtagcc gggcaaacta
       61 aacgctgtca cgatcggatg acgaaagcca ttgctgcgtt cccacatgca gcaatggcgg
      121 cactgacgga acttcttggg caaaaagaag agaacagttg gcgcattatg ctaatgacaa
      181 tgcttatctc acaaccagca ctggcagaac aggtccttcc ctggctctcg acacccgcag
      241 ttgccgtact gaaatcatac cagcaacaac tgacacagcc ctcaaaccat gcctgcgccg
      301 atctactgcc tgccgtatta gtctcaccgc cctggctttc gaaaaagaaa aaatctgtaa
      361 tgccggtatt ggatttaacg ccattaccgt tggagtcatg ctgtacattg actgagacgg
      421 cagaaaaaga gattcataca aggcatcgct ggcacgcaca tcaaatagac atcggccaaa
      481 aagaggatat tcagaactac ctggcacgtc tgggtttcaa tcgctggaat aatggccagt
      541 atatgaaagc gtctgatgct gttgtagaac tct
//
LOCUS       DADLTD010000424          560 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.428, whole genome shotgun sequence.
ACCESSION   DADLTD010000424 DADLTD010000000
VERSION     DADLTD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.428"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>560
                     /locus_tag="JN701_004284"
     CDS             <1..>560
                     /locus_tag="JN701_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7366492.1"
                     /translation="EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDD
                     DLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKL
                     AAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVA
                     LEVEQRVLAHPLYPVLTSMPGVGVRT"
BASE COUNT          120 a          184 c          147 g          109 t
ORIGIN      
        1 tgaagctaaa actgatgctc gtgacgctgc catcattgcc gaagctgccc gtaccctgcc
       61 tcacgcgcta cgcacgctga aactggctga cgagcaaatc gccgaactct ccatgctctg
      121 cggcttcgat gatgatcttg ccgcacagac aacgcaggcc agcaaccgta tccgcggcct
      181 tctgacccag atacatccgg cactggagcg cgttctcggt ccgagacttg agcacccggc
      241 ggtactcgat cttctccagc gatatccctc accagaaaaa ctcgcttcgc tgggtgagaa
      301 gaagctggca gcccagctct gcaaacttgc gcctcgtctg ggtaaacgcc ttgcagcaga
      361 catagctcag gcactggccg aacaaaccgt cgtcgttccc ggcacgaatg ccgctgccgt
      421 agtactgcca cgtctggcac tccagctcat cacgctgcgt aagcaaagag acgaggtggc
      481 gcttgaggta gaacagcgag ttcttgctca ccctctttac ccggtcctga ccagtatgcc
      541 cggagtcggt gtcaggaccg
//
LOCUS       DADLTD010000425          558 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.429, whole genome shotgun sequence.
ACCESSION   DADLTD010000425 DADLTD010000000
VERSION     DADLTD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.429"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            2..>558
                     /gene="fimZ"
                     /locus_tag="JN701_004285"
     CDS             2..>558
                     /gene="fimZ"
                     /locus_tag="JN701_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAY7366493.1"
                     /translation="MIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRP
                     VDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKC
                     NDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGL
                     SNKEIADKLLLSNKTVSAHKSNIYG"
BASE COUNT          180 a          102 c          106 g          170 t
ORIGIN      
        1 ggtgatcatt atggatactc atcctatcat cagaatgtct attgaagttc tgttgcaaaa
       61 aaacagtgaa ttgcagattg tcctgaaaac ggatgattac cgcataacca tcgattatct
      121 ccgaacccgt cctgttgatt taatcattat ggatatagac ttgcccggaa cagacggttt
      181 taccttcctg aaaaggatca aacaaatcca gagcacagtg aaagtgttat ttttatcatc
      241 gaaatcagaa tgcttttatg ctggcagagc gatacaagct ggtgctaacg gttttgtcag
      301 taaatgcaat gatcagaatg atatttttca tgccgttcag atgatcctct ccggatacac
      361 gttttttccc agcgaaacgc ttaactatat aaaaagcaat aaatgtagta cgaatagttc
      421 aacggtcact gtgctatcta atcgtgaagt gaccatatta cgttatctgg ttagcggatt
      481 atctaataaa gaaattgccg ataagttatt gcttagcaat aaaacagtta gtgcgcataa
      541 atctaatatt tatggcaa
//
LOCUS       DADLTD010000426          556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.430, whole genome shotgun sequence.
ACCESSION   DADLTD010000426 DADLTD010000000
VERSION     DADLTD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.430"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>556
                     /gene="dnaE"
                     /locus_tag="JN701_004286"
     CDS             <1..>556
                     /gene="dnaE"
                     /locus_tag="JN701_004286"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time; main
                     replicative polymerase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7366494.1"
                     /translation="IPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQPDCFEDMI
                     ALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYGIILYQEQV
                     MQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAELAMKIFDLV
                     EKFAGYGFNKSHSAAYALVSYQTLW"
BASE COUNT          143 a          131 c          158 g          124 t
ORIGIN      
        1 atcccgctgg atgacaagaa aagcttcgac atgctgcaac gctcggaaac cacggcggta
       61 ttccagcttg aatcgcgcgg catgaaggat ctgatcaagc gtctgcaacc tgactgcttc
      121 gaagatatga tcgcactggt ggcgctgttc cgcccaggtc cgttgcaatc agggatggtg
      181 gataacttta tcgaccgtaa acatggtcgc gaagaaatct cctatccgga cgtacagtgg
      241 cagcatgaaa gcctgaaacc ggtactggag ccaacctacg gcatcatcct gtatcaggaa
      301 caggtcatgc agattgcgca ggtgctttct ggttataccc tcggtggcgc ggatatgctg
      361 cgtcgtgcga tgggtaagaa aaagccggaa gagatggcta agcagcgttc tgtatttgct
      421 gaaggtgcag aaaagaacgg agtcaacgcc gaactggcga tgaaaatctt cgacctggtg
      481 gagaaattcg cgggttacgg atttaacaaa tcgcactctg cggcctatgc tttggtgtca
      541 tatcaaacgt tatggc
//
LOCUS       DADLTD010000427          544 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.431, whole genome shotgun sequence.
ACCESSION   DADLTD010000427 DADLTD010000000
VERSION     DADLTD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.431"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(50..>544)
                     /locus_tag="JN701_004287"
     CDS             complement(50..>544)
                     /locus_tag="JN701_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY7366495.1"
                     /translation="SVDYNIEGFGFVGAYSKSDRTNEQAGDGYGDNAEVWSLAAKYDA
                     NNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFDFGLRPSLGYVYSKGKDLYA
                     RDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLDKDDAAITDAATDDQFAVGI
                     IYQF"
BASE COUNT          150 a          119 c          111 g          164 t
ORIGIN      
        1 tcaggctagt gttatgtttt acagtaacac gccgcggcgc gttggctgat tagaactggt
       61 agatgatacc aactgcaaac tggtcatcag ttgcggcatc ggtaatcgct gcatcgtctt
      121 tatccagcag gttaaatttg tatgctgtgt agacgttcat gttcttattg aagtagtacc
      181 aggtaccaac ttcgatataa tttacgcggt cagcatcaac acctttatgt ccatcacgag
      241 cataaagatc tttgcctttg gaatatacgt agcctaaaga cggacgtaaa ccgaagtcga
      301 actgatactg tacaacagct tcaaagtttt gggttttatt tgcaaaatga tcattagcca
      361 aaacggtcat gttgcgggtt tcaccgtaca tcattgctgc atagatatta tttgcatcat
      421 acttggctgc taatgaccac acttcagcgt tatcaccgta gccgtcacca gcttgttcat
      481 tagtacgatc agatttgcta tatgcaccta caaaaccaaa accttcgatg ttgtagtcta
      541 caga
//
LOCUS       DADLTD010000428          529 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.432, whole genome shotgun sequence.
ACCESSION   DADLTD010000428 DADLTD010000000
VERSION     DADLTD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.432"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..408
                     /gene="csgD"
                     /locus_tag="JN701_004288"
     CDS             <1..408
                     /gene="csgD"
                     /locus_tag="JN701_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY7366496.1"
                     /translation="IKILLLNTPEDYPYRDIENWPHINGVFYSMEDQERVVNGLQGVL
                     RGECYFTQKLASYLITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFIS
                     ENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            413..>529
                     /locus_tag="JN701_004289"
     CDS             413..>529
                     /locus_tag="JN701_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly protein CsgE"
                     /protein_id="HAY7366497.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSI"
BASE COUNT          150 a          121 c          119 g          139 t
ORIGIN      
        1 atcaaaatat tgttgctaaa tacgcctgaa gattacccgt accgcgacat tgaaaactgg
       61 cctcatatca acggcgtttt ttattccatg gaggatcaag aacgtgttgt caatgggttg
      121 caaggcgtcc tgcgcggcga atgctacttt acgcaaaagc ttgccagcta cttgattacg
      181 cattcaggta actatcgtta taacagcacg gaatcagccc tccttactca tcgggaaaaa
      241 gagatcctga ataagctgcg tatcggcgcg tctaataacg agatcgctcg ttcgttgttc
      301 atcagcgaaa atacggttaa aacgcatctt tataatcttt tcaagaagat agccgtaaaa
      361 aaccggacac aagcggtttc ctgggcaaac gataacctca ggcgataaag ccatgaaacg
      421 ttatttacgc tggattgtgg cggcagaatt tctgttcgcc gcagggaatc ttcacgccgt
      481 tgaggtagaa gtcccgggat tgctaactga ccatactgtt tcatctatt
//
LOCUS       DADLTD010000429          510 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.433, whole genome shotgun sequence.
ACCESSION   DADLTD010000429 DADLTD010000000
VERSION     DADLTD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.433"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>510
                     /locus_tag="JN701_004290"
     CDS             <1..>510
                     /locus_tag="JN701_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HAY7366498.1"
                     /translation="LRDIGVEAVTKMLACGTRILGVKEYICDKPECPHVRYVTNSCGS
                     RACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVE
                     NLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAMR
                     SRWMWNMRQL"
BASE COUNT          111 a          136 c          157 g          106 t
ORIGIN      
        1 ctgcgcgata tcggggttga ggctgtcaca aaaatgctgg cctgcggcac gcggatactg
       61 ggagtgaagg aatacatctg cgataaacct gagtgccccc acgtcagata cgtcactaac
      121 tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct gtggatagcg
      181 acacagctga atcgtcttcc tgactgcgac tgggtacacc tggtcttcac cctgccggac
      241 acgctgtggc cggtgttcga aagcaaccgg tggctgctga atgacgtgtg ccgtctggcg
      301 gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg aacccggtat cttctgcgcc
      361 atccacacgt atggccgtcg tctcaactgg catccgcatg tacatgtgtc tgtaacctgt
      421 ggaggtctga ataagcatgg tcagtggaaa aagctgagct tcctgaaaga cgcgatgcgt
      481 tcacggtgga tgtggaatat gcggcagctg
//
LOCUS       DADLTD010000430          506 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.434, whole genome shotgun sequence.
ACCESSION   DADLTD010000430 DADLTD010000000
VERSION     DADLTD010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.434"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(231..482)
                     /gene="dgcZ"
                     /locus_tag="JN701_004291"
                     /pseudo
     CDS             complement(231..482)
                     /gene="dgcZ"
                     /locus_tag="JN701_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
BASE COUNT          131 a          120 c          109 g          146 t
ORIGIN      
        1 tgggatgact aatgaacgga gataatccct cacctaaccg gccccttgtt acagttgtgt
       61 acaaggggcc tgatttttat gacggcgaaa aaaaaccgcc agtaaaccgg cggtgaatgc
      121 ttgcatggat agatttgtgt tttgctttta cgctaacagg cattttcctg cactgataac
      181 gaatcgttga cacagtagca tcagttttct caatgaatgt taaacggagc ttaaactcgg
      241 ttaatcacat tttgttcgtc aataaacata cagcgatttc ttcccgtttg cttaccctca
      301 tacattgccc ggtctgctct tccaatgacc acatccagag gctcttcagg aaatgcgcga
      361 ctgacacctg ctgtcacggt aatgttgata tgcccttcag aatgtgtgat ggcatggtta
      421 tcgactaact ggcaaattct gacacctgca cgacatgctt cttcatcatt agccgctttg
      481 aggtgtactg gcaatagcgg acacta
//
LOCUS       DADLTD010000431          468 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.435, whole genome shotgun sequence.
ACCESSION   DADLTD010000431 DADLTD010000000
VERSION     DADLTD010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.435"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            109..468
                     /locus_tag="JN701_004292"
                     /pseudo
     CDS             109..468
                     /locus_tag="JN701_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          121 a          115 c          110 g          122 t
ORIGIN      
        1 tttttaacgt aaggtgagag gatttttaag tgtatccata gattaaaaat gacaatgcct
       61 ccaattagtt gaattagggg ttataatacc cgcttttaag gttctttcat ggtcacagtt
      121 aactttcatt gcccccgttg ccagtccgtt caggtttacc gtcacgggca gaacccaaaa
      181 ggacatgatc gattccgttg ccgtgactgt caccgggtgt ttcagctcac ttactgttac
      241 gaggcccgta aaccgggagt taaaaaccaa atcactgaaa tggcttcaac ggtgccggag
      301 ttcgcgatac tgcccgtacg ctgaaaatcg gcatcaatac cgtcatacgg acattaaaaa
      361 gctcccgccc cggcggataa catctgcgcc agtcgctcac gctgatgttg cccttatctg
      421 tgaacttgat gggcagtgga gcttcgttgg cagcaaggcg caacaaca
//
LOCUS       DADLTD010000432          451 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.436, whole genome shotgun sequence.
ACCESSION   DADLTD010000432 DADLTD010000000
VERSION     DADLTD010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.436"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>451
                     /gene="ipaH9.8"
                     /locus_tag="JN701_004293"
     CDS             <1..>451
                     /gene="ipaH9.8"
                     /locus_tag="JN701_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7366499.1"
                     /translation="LSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATES
                     CEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVRTLH
                     FVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMV"
BASE COUNT          100 a          129 c          117 g          105 t
ORIGIN      
        1 cctttccgat accgtctctg cacgcaatac ctccggattc cgtgaacagg tcgctgcatg
       61 gctggaaaaa ctcagtgcct ctgcggagct tcgacagcag tctttcgctg ttgctgctga
      121 tgccactgag agctgtgagg accgtgtcgc gctcacatgg aacaatctcc ggaaaaccct
      181 cctggtccat caggcatcag aaggcctttt cgataatgat accggcgctc tgctctccct
      241 gggcagggaa atgttccgcc tcgaaattct ggaggacatt gcccgggata aagtcagaac
      301 tctccatttt gtggatgaga tagaagtcta cctggccttc cagaccatgc tcgcagagaa
      361 acttcagctc tccactgccg tgaaggaaat gcgtttctat ggcgtgtcgg gagtgacagc
      421 aaatgacctc cgcactgccg aagccatggt c
//
LOCUS       DADLTD010000433          450 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.437, whole genome shotgun sequence.
ACCESSION   DADLTD010000433 DADLTD010000000
VERSION     DADLTD010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.437"
                     /strain="EG0380"
                     /db_xref="taxon:623"
BASE COUNT          144 a           69 c           81 g          156 t
ORIGIN      
        1 cgttacttat tccaattcgt tactataaat atggcaaaaa tattacaaca aacagcggtt
       61 aaaaaatttg gtgaagattt tttgtaataa tcctaattag ttatgggaat ttcgcttgat
      121 gtatctgcat aacaaaaaca atatgcgcca tttttgcatt actaataaga aaaagcaaaa
      181 aacatcgata ttcatttttg gtcaatgggt tttctggcct atcgttgtta ttgtgctact
      241 ggttatcatt ttcatcttgc cagcatattg gagcgtgatc aattttgatc agctgtgaag
      301 gtggctgatg tgtagtgata caaaaactta cgttatatca ctgctaatga gatatccgtc
      361 atctgaacac ttatgtcttt aattattctc gtggttcact ataggcaata agcacaaaag
      421 tgtaggatgt tacaagaatg attaggactc
//
LOCUS       DADLTD010000434          443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.438, whole genome shotgun sequence.
ACCESSION   DADLTD010000434 DADLTD010000000
VERSION     DADLTD010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.438"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(357..>443)
                     /locus_tag="JN701_004294"
     CDS             complement(357..>443)
                     /locus_tag="JN701_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HAY7366500.1"
                     /translation="RQMQARRVLQQMSDERLRDIGLRREDVE"
BASE COUNT          119 a          102 c           83 g          139 t
ORIGIN      
        1 ttcattgtat cctctggcag gtgtataatt agcgaattga tactagcaac gaagcttcgg
       61 ttatctattt gcagagaatt gtttcattat gaactgtgag cgcgataact aatttttgat
      121 acaaaaattt cattaatgtg atctaaaacg aatcgcttta ttcaccgctg atgattttgt
      181 tctattcctt tttgtgacag atcccccgga cgcatagatg atgaccccca gagagacagc
      241 tcaggtcatt atctgtatgt aaatcagaaa aatatgttgt aaacgacttt tatggttgga
      301 ttttgtagca attttgaatg cagacaggca gcatttgcca acacgccgcc catagttcac
      361 tccacatcct ccctgcgtaa cccgatatcc ctcaaccgtt catcactcat ctgctgtaac
      421 acccgtcggg cctgcatttg ccg
//
LOCUS       DADLTD010000435          432 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.439, whole genome shotgun sequence.
ACCESSION   DADLTD010000435 DADLTD010000000
VERSION     DADLTD010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.439"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..334)
                     /locus_tag="JN701_004295"
     CDS             complement(<1..334)
                     /locus_tag="JN701_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7366501.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPES"
BASE COUNT          139 a           95 c          100 g           98 t
ORIGIN      
        1 tactttccgg taaaaataaa aacggtaaat agagcgcaat atcggaaata acatcgccag
       61 tttcattcaa aatcgccccc agacgtgttt gctgattgca ctcacgcgcc aacatgccat
      121 ccagcgcatt gagcgccata cggataaaaa gcacgatggg caatagcaaa aagaggatgg
      181 gttgtgccac caacaccaac agcaatccgg taaaaagaga aagcgccagt gccgtaagag
      241 tgatgtgatt cgctgtaacg tggtgcttat aaagccaaaa catcgtcggc cttaacagcg
      301 actgaaagag cggttttatc tggtagagcg tcatttatca ttccgtgata ttcagtttgc
      361 tctatggtgc atcaattgtg atggcggaga tatcacaacg cggagcatca acgtaaattc
      421 ggaatatatc aa
//
LOCUS       DADLTD010000436          428 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.440, whole genome shotgun sequence.
ACCESSION   DADLTD010000436 DADLTD010000000
VERSION     DADLTD010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.440"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>428
                     /locus_tag="JN701_004296"
     CDS             <1..>428
                     /locus_tag="JN701_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY7366502.1"
                     /translation="GLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISEDGNTAW
                     SAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGRYITQDPIG
                     LEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQ"
BASE COUNT          111 a           99 c          135 g           83 t
ORIGIN      
        1 cgggctgacg gtggagcggc tggcggcaca gatagagccg gtttatttgc ctgagcgaaa
       61 aatacatctt tatcactgtg accaccgggg cctgccgctg gcgcttatca gtgaagacgg
      121 caatacggcg tggagcgcgg aatatgatga atggggcaac cagcttaatg aggagaaccc
      181 gcatcacctg caccagccgt accggctgcc ggggcagcag tatgataagg agtcggggct
      241 gtactacaac cggaaccggt actacgatcc gttgcagggg cggtatatca ctcaggaccc
      301 gatagggctg gaggggggat ggagtctgta tgcgtatccg ctgaatccgg tgaatggtat
      361 tgatccatta gggttaagtc ccgcagatgt agcgctaata agaagaaaag atcaactaaa
      421 ccatcaaa
//
LOCUS       DADLTD010000437          427 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.441, whole genome shotgun sequence.
ACCESSION   DADLTD010000437 DADLTD010000000
VERSION     DADLTD010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.441"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            44..380
                     /locus_tag="JN701_004297"
                     /pseudo
     CDS             44..380
                     /locus_tag="JN701_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          142 a           75 c           95 g          115 t
ORIGIN      
        1 tggagtagtg cttattttcg actcaatttc tactgattag attatggtta tcagtacagt
       61 gggttatgaa aaacggactg gatctgccag cagtgcttct attattattc aaaaagaact
      121 tcagccggac aatgctataa tccagggaaa ggctataagc tctgttggac ggaatgtgga
      181 aatcagcact ggtaaaaagg ggaactgaca ttaacacaag aaggattcct gcaagcaact
      241 gcttatctgt ccggaacgca gaattaaagc atgaccacac aggagccaga atactgtggt
      301 cgtacgcatt cagtattgtg acaactaata gaaataaaaa tctggccggt gcgaatatta
      361 aacgatgcga taccgtatag tgtaagtcaa aagtaacggg gtatttaaac catcttttca
      421 tatttat
//
LOCUS       DADLTD010000438          413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.442, whole genome shotgun sequence.
ACCESSION   DADLTD010000438 DADLTD010000000
VERSION     DADLTD010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.442"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>413
                     /locus_tag="JN701_004298"
     CDS             <1..>413
                     /locus_tag="JN701_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7366503.1"
                     /translation="GKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQN
                     RAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQ
                     SCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKL"
BASE COUNT          109 a          101 c          111 g           92 t
ORIGIN      
        1 cgggaaaatg gcttcctggc tggttgagtg gaaaaatgcc actgaaacgc aatggcttaa
       61 agatgccccg tcacagccat tgcaacagtc actgaaagac cttgagcggg cttacaaaaa
      121 cttcttccag aatcgggctg cttttccccg attcaaaaag cggggacaga atgatgtatt
      181 ccgctacccg cagggcgtta agctcgatca ggaaaacagc cgtatttttc tgccgaaact
      241 gggctggatg cgctaccgga acagccgtca ggtcacgggt gttgtgaaaa atgtcactgt
      301 cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag tatcaactcc
      361 ggttcaccct tcagcatcaa tggtcgggct ggatgctggc gtggctaaac tcg
//
LOCUS       DADLTD010000439          411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.443, whole genome shotgun sequence.
ACCESSION   DADLTD010000439 DADLTD010000000
VERSION     DADLTD010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.443"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..389)
                     /locus_tag="JN701_004299"
     CDS             complement(<1..389)
                     /locus_tag="JN701_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7366504.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPH"
BASE COUNT          104 a          102 c          126 g           79 t
ORIGIN      
        1 cagtgcggat gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg
       61 acagcctcac ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg
      121 cgattgcccg gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc
      181 tcaagcgcca tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt
      241 agcgttacag tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc
      301 cccagagaag tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt
      361 ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc cggattctta c
//
LOCUS       DADLTD010000440          400 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.444, whole genome shotgun sequence.
ACCESSION   DADLTD010000440 DADLTD010000000
VERSION     DADLTD010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.444"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..191)
                     /locus_tag="JN701_004300"
     CDS             complement(<1..191)
                     /locus_tag="JN701_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 domain-containing protein"
                     /protein_id="HAY7366505.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICA"
     gene            complement(203..>400)
                     /locus_tag="JN701_004301"
     CDS             complement(203..>400)
                     /locus_tag="JN701_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366506.1"
                     /translation="TPAGKNGADEATYVYLNGTRLLVELPYQTVQEIISEAEKARQVN
                     GDEPYIEIICMDSEAEIQKAD"
BASE COUNT           99 a           98 c           87 g          116 t
ORIGIN      
        1 gcacagatca gctcggaaag ttcgttaagt gcatccagat catcagcgta aaaagccacg
       61 tcatacagac ttcggattgc tctggtcaat gagtcacggg ccgcacgttc agcatgagcg
      121 cctgatgcac ttaagcgaaa ataaaaacgc tcaagtgctt tgttaatgag agttttatat
      181 tctttgccca tcacaacgcc ctttaatctg ctttctgtat ttcagcttct gaatccatac
      241 aaataatttc gatatagggt tcatcgccat taacctggcg tgccttttca gcttcgctaa
      301 tgatttcttg tacggtctgg tacggaagtt ccacaagcag tcgcgtgccg ttcagataaa
      361 cgtaagtggc ttcgtcggct ccgtttttac ccgccggagt
//
LOCUS       DADLTD010000441          384 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.445, whole genome shotgun sequence.
ACCESSION   DADLTD010000441 DADLTD010000000
VERSION     DADLTD010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.445"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..>384)
                     /locus_tag="JN701_004302"
     CDS             complement(<1..>384)
                     /locus_tag="JN701_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAY7366507.1"
                     /translation="PHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGI
                     QLIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRI
                     CTFATVIGGTLLSLPLMWQLADIIM"
BASE COUNT          106 a           96 c          102 g           80 t
ORIGIN      
        1 ccattatgat atctgccagt tgccacatca gcggaaggct tagcaaggtg ccgccgatga
       61 ccgttgcgaa ggtgcagatc cgcaaacacc agatcgcttt aggggtgttc aggcgtaaaa
      121 agaagagatt gttttcggca taaatgtagt tggcaacgat ggagctgaag gcaaacagaa
      181 taaccacgag ggtaacaaac tcagcacccc aggaacccat tagcacccgc atcgccttct
      241 ggataagctg aataccttcc agcggcatgt aggttgtgtc gttacccgcc agtaatatca
      301 gcatggcgct tgccgtacag atgaccaggg tgtcgataaa aatgccaatc atctggacaa
      361 tcccttgcgc tgccggatgc ggag
//
LOCUS       DADLTD010000442          381 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.446, whole genome shotgun sequence.
ACCESSION   DADLTD010000442 DADLTD010000000
VERSION     DADLTD010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.446"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>381
                     /locus_tag="JN701_004303"
     CDS             <1..>381
                     /locus_tag="JN701_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7366508.1"
                     /translation="SHITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLK
                     KPPIAASRLAHYNGEASLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRY
                     FGFLANRVCGEKLPQVYRALGMDK"
BASE COUNT           99 a           90 c          125 g           67 t
ORIGIN      
        1 tgtcacatat cacgacggaa tcacagtgga gaagcctggt gctgaaagcc ggaggaaaat
       61 actggcatgt gtacatgtcg aaaaaaacgg ccggcgggag gaatacggcg cgctacctgg
      121 gtcgttatct gaagaagccg ccgatagcgg cctcccggct ggcacattac aacggagagg
      181 cgagcctgag cttccgttac ctggaccaca aaacgggaga aacggcgacg gaaacgctga
      241 cacagcgtga gctggtcgcg aggctgaaac agcacatccc ggagaagttt tttaagatgg
      301 tgaggtactt cgggttcctt gccaaccggg tgtgtggaga gaagctgccg caggtgtacc
      361 gtgctctggg gatggataaa c
//
LOCUS       DADLTD010000443          368 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.447, whole genome shotgun sequence.
ACCESSION   DADLTD010000443 DADLTD010000000
VERSION     DADLTD010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.447"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            17..>368
                     /gene="leuE"
                     /locus_tag="JN701_004304"
     CDS             17..>368
                     /gene="leuE"
                     /locus_tag="JN701_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HAY7366509.1"
                     /translation="MVLVPGPNTLFVLKNSVSSGMKGGYLAACGVFIGDAVLMFLAWA
                     GVATLIKTTPILFNIVRYLGAFYLLYLESKILYATLKGKNNEAKSDEPQYGAIFKRAL
                     ILSLTNPKAILFYVS"
BASE COUNT           87 a           72 c           93 g          116 t
ORIGIN      
        1 ggttggggcc atttttattg tgttggtgcc tgggccaaat accctgtttg tactcaaaaa
       61 tagcgtcagt agcggtatga aaggcggtta tcttgcggcc tgcggtgtat ttattggcga
      121 tgcagtattg atgtttctgg catgggctgg agtggcgaca ttaattaaga ccaccccgat
      181 attattcaac attgtacgtt atcttggtgc gttttatttg ctctatctgg agagtaaaat
      241 tctctacgcg accctgaagg gtaaaaataa cgaggccaaa tccgatgagc cccaatacgg
      301 ggccattttt aaacgcgcgt taattttgag cctgactaat ccgaaagcca ttttgttcta
      361 tgtgtcgt
//
LOCUS       DADLTD010000444          362 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.448, whole genome shotgun sequence.
ACCESSION   DADLTD010000444 DADLTD010000000
VERSION     DADLTD010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.448"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..355)
                     /locus_tag="JN701_004305"
     CDS             complement(<1..355)
                     /locus_tag="JN701_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY7366510.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKQARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIK"
BASE COUNT           87 a           82 c           89 g          104 t
ORIGIN      
        1 gcttaatttt gctttcacat ttttgtaagt cttctgccgg attgtgcgac gtctgcaata
       61 tcacgtcgaa accttcctct tcagctttgg cggtgatggc atgcaaaact tcagagaaaa
      121 acggattacc cgccgtagtt ttggtcgaac gggtagaaat caccataatg gcatcaaaac
      181 ccgaagaggt cagcgcgcgg gcctgcttgt ttggctgata ctgtaattct tcaatggccc
      241 gtaacacttt ttcgcgcgct tccggggaga tattagtttg cttattcagc acgcgtgata
      301 cggtggattt tgatacgcct gcaaccctgg caatatcata aatagtaggg gacataggtc
      361 ag
//
LOCUS       DADLTD010000445          338 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.449, whole genome shotgun sequence.
ACCESSION   DADLTD010000445 DADLTD010000000
VERSION     DADLTD010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.449"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            88..>338
                     /locus_tag="JN701_004306"
     CDS             88..>338
                     /locus_tag="JN701_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366511.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLN"
BASE COUNT          112 a           84 c           70 g           72 t
ORIGIN      
        1 ggctgcaatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
       61 aatataaaaa acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc
      121 acaccgaaac agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc
      181 ggcatgcttt gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa
      241 actacaaaaa gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa
      301 ctaccaggaa cttatcaggt tgatatctgg ctgaataa
//
LOCUS       DADLTD010000446          335 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.450, whole genome shotgun sequence.
ACCESSION   DADLTD010000446 DADLTD010000000
VERSION     DADLTD010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.450"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            complement(<1..>335)
                     /locus_tag="JN701_004307"
     CDS             complement(<1..>335)
                     /locus_tag="JN701_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7366512.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIA"
BASE COUNT           79 a           96 c           90 g           70 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatacg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggta
//
LOCUS       DADLTD010000447          331 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.451, whole genome shotgun sequence.
ACCESSION   DADLTD010000447 DADLTD010000000
VERSION     DADLTD010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.451"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>331
                     /locus_tag="JN701_004308"
     rRNA            <1..>331
                     /locus_tag="JN701_004308"
                     /product="16S ribosomal RNA"
BASE COUNT           85 a           82 c           95 g           69 t
ORIGIN      
        1 ttacgaccag ggctacacac gtgctacaat ggcgcataca aagagaagcg acctcgcgag
       61 agcaagcgga cctcataaag tgcgtcgtag tccggattgg agtctgcaac tcgactccat
      121 gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg gtgaatacgt tcccgggcct
      181 tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa agaagtaggt agcttaacct
      241 tcgggagggc gcttaccact ttgtgattca tgactggggt gaagtcgtaa caaggtaacc
      301 gtaggggaac ctgcggttgg atcacctcct t
//
LOCUS       DADLTD010000448          326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.452, whole genome shotgun sequence.
ACCESSION   DADLTD010000448 DADLTD010000000
VERSION     DADLTD010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.452"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>326
                     /locus_tag="JN701_004309"
     CDS             <1..>326
                     /locus_tag="JN701_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7366513.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DADLTD010000449          318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.453, whole genome shotgun sequence.
ACCESSION   DADLTD010000449 DADLTD010000000
VERSION     DADLTD010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.453"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>318
                     /locus_tag="JN701_004310"
     CDS             <1..>318
                     /locus_tag="JN701_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7366514.1"
                     /translation="SFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQL
                     LWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYS
                     ALRP"
BASE COUNT           82 a           76 c           99 g           61 t
ORIGIN      
        1 gtcgttctcg ccggacagca ggctcaccat gaaagcgctg gaaatggcat gggaaacccg
       61 tggtaagccc ggcggggtga tgttccacag cgatcagggc agtcattata cgagcaggca
      121 gttccggcag ttattgtggc gataccagat cagacagagt atgagccggc gcggaaactg
      181 ctgggataac agcccaatgg aacgcttctt caggagtctg aagaacgaat ggatgccggt
      241 ggtgggttac gtaagcttca gcgaggcagc tcacgccata acggactata tcgttggata
      301 ttacagcgca ctaagacc
//
LOCUS       DADLTD010000450          280 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.454, whole genome shotgun sequence.
ACCESSION   DADLTD010000450 DADLTD010000000
VERSION     DADLTD010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.454"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            46..243
                     /locus_tag="JN701_004311"
     CDS             46..243
                     /locus_tag="JN701_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366515.1"
                     /translation="MRKILQQKGDPDLIQCDYHLCNKQFLPEKAFTGELIYMHGNMIA
                     RKKRKYCGAACAEKDKMAHEL"
BASE COUNT          104 a           50 c           56 g           70 t
ORIGIN      
        1 aggcagacca tcagtcatac cgggattaaa aaatggctgt ggagaatcag aaaaattctc
       61 cagcaaaaag gtgacccgga tttaatacaa tgtgattacc atctctgcaa taaacagttt
      121 ttacctgaaa aagcttttac tggcgagctt atttatatgc atggaaacat gattgccaga
      181 aagaaacgaa aatattgcgg tgcagcttgt gccgaaaaag acaagatggc acacgaactt
      241 taattaactg aatattcgaa actgaattta tgccagcaat
//
LOCUS       DADLTD010000451          277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.455, whole genome shotgun sequence.
ACCESSION   DADLTD010000451 DADLTD010000000
VERSION     DADLTD010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.455"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            109..>277
                     /locus_tag="JN701_004312"
     CDS             109..>277
                     /locus_tag="JN701_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7366516.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQV"
BASE COUNT           79 a           62 c           65 g           71 t
ORIGIN      
        1 gagacagatg ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc
       61 atgggtgagc tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat
      121 aacacattgt acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc
      181 aattcagaac ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat
      241 ctttgtaaac gtctcaggtc aaagtcatcg caggtca
//
LOCUS       DADLTD010000452          276 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.456, whole genome shotgun sequence.
ACCESSION   DADLTD010000452 DADLTD010000000
VERSION     DADLTD010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.456"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            44..>276
                     /locus_tag="JN701_004313"
     CDS             44..>276
                     /locus_tag="JN701_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7366517.1"
                     /translation="MSRKNQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEAR"
BASE COUNT           83 a           65 c           66 g           62 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaaacca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatccct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac tggaatctga
      241 aattctgcaa ctgcgtaagg cgttaaatga agctcg
//
LOCUS       DADLTD010000453          272 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.457, whole genome shotgun sequence.
ACCESSION   DADLTD010000453 DADLTD010000000
VERSION     DADLTD010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.457"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            5..272
                     /locus_tag="JN701_004314"
                     /pseudo
     CDS             5..272
                     /locus_tag="JN701_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           87 a           62 c           62 g           61 t
ORIGIN      
        1 aataatgagc agaaaaaccc aacgttactc tacggagttt aaagccgaag ccgtcaaaac
       61 ggttcctgaa aatcagcttt cgatcagtga aggcgcttcc cgattatctg ttcctgaagg
      121 cactttagga caatgggtta ccgctgcaag aaaagggctc aatacaccag gctcccgcac
      181 agtggctgag ctttatgcaa ctgcgtaagg ctttaaatga agcacgactt gagcgagata
      241 tattaaaaaa agcaacagcg tattttgccc ag
//
LOCUS       DADLTD010000454          260 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.denovo.458, whole genome shotgun sequence.
ACCESSION   DADLTD010000454 DADLTD010000000
VERSION     DADLTD010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.denovo.458"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..>260
                     /locus_tag="JN701_004315"
     CDS             <1..>260
                     /locus_tag="JN701_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366518.1"
                     /translation="NWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTN
                     FHQIIMDTISTPLASLGSVVGWAYVIFVPLLCEPPRESWRLN"
BASE COUNT           50 a           70 c           63 g           77 t
ORIGIN      
        1 caattgggtc attaaactgc ccgacagcgt acctgcttca gtatcgcgtt ccttctcggc
       61 attaattccc ggctttatta ttctttccgt gatggggatt attgcctggg cgttgaatac
      121 ctggggcacc aacttccatc agatcattat ggataccatc tcaaccccac tggcatcgtt
      181 gggtagcgtg gtgggctggg cctatgtgat ctttgttcca ctgctctgtg aaccgccccg
      241 ggaatcctgg agactaaact
//
LOCUS       DADLTD010000455         8530 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.guided.173, whole genome shotgun sequence.
ACCESSION   DADLTD010000455 DADLTD010000000
VERSION     DADLTD010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.guided.173"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..178
                     /locus_tag="JN701_004316"
     CDS             <1..178
                     /locus_tag="JN701_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HAY7366519.1"
                     /translation="ASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEF
                     AAGKYDITRPKAAG"
     gene            complement(385..462)
                     /locus_tag="JN701_004317"
                     /pseudo
     CDS             complement(385..462)
                     /locus_tag="JN701_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            485..1300
                     /gene="sul2"
                     /locus_tag="JN701_004318"
     CDS             485..1300
                     /gene="sul2"
                     /locus_tag="JN701_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7366520.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1361..2164
                     /locus_tag="JN701_004319"
                     /note="aph(3'')-Ib"
     CDS             1361..2164
                     /locus_tag="JN701_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7366521.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2164..3000
                     /locus_tag="JN701_004320"
     CDS             2164..3000
                     /locus_tag="JN701_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7366522.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            2904..3107
                     /locus_tag="JN701_004321"
                     /pseudo
     CDS             2904..3107
                     /locus_tag="JN701_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein (MobL-like)"
     gene            3387..3629
                     /locus_tag="JN701_004322"
     CDS             3387..3629
                     /locus_tag="JN701_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY7366523.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(3661..4311)
                     /gene="tetR(A)"
                     /locus_tag="JN701_004323"
     CDS             complement(3661..4311)
                     /gene="tetR(A)"
                     /locus_tag="JN701_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY7366524.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKQALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            4417..5616
                     /gene="tet(A)"
                     /locus_tag="JN701_004324"
     CDS             4417..5616
                     /gene="tet(A)"
                     /locus_tag="JN701_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY7366525.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(5547..5984)
                     /locus_tag="JN701_004325"
                     /pseudo
     CDS             complement(5547..5984)
                     /locus_tag="JN701_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(6446..6730)
                     /locus_tag="JN701_004326"
     CDS             complement(6446..6730)
                     /locus_tag="JN701_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="HAY7366526.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRR
                     KVLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            6929..>8530
                     /locus_tag="JN701_004327"
     CDS             6929..>8530
                     /locus_tag="JN701_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAY7366527.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAES
                     GHMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGA
                     ERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEAL
                     KPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGR
                     GIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEH
                     GDTGRRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERP
                     ERVAGVALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRT
                     LQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIR
                     PAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQ
                     IARTLASEYDADPASADSRHYGRLAG"
BASE COUNT         1630 a         2602 c         2579 g         1719 t
ORIGIN      
        1 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
       61 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
      121 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
      181 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
      241 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
      301 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
      361 atgtatgctc ttctgctcct gcagctaatg gatcaccgca aacaggttac tcgcctgggg
      421 attccctttc gacccgagca tccgtatgat actcatgctc gattattatt attatagaag
      481 ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc
      541 cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc
      601 cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt
      661 ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg
      721 cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg
      781 tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc
      841 gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg
      901 cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc
      961 ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt
     1021 ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg
     1081 gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct
     1141 cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc
     1201 cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc
     1261 ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc
     1321 tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa ctaatatttt
     1381 ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg gtgattttgt
     1441 ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc gccgcggtga
     1501 gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg cttgccccga
     1561 ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg caattccggg
     1621 agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt caatggggca
     1681 gcaacttggc gctgttcaca gcctatcggt tgatcaatgt ccgtttgagc gcaggctgtc
     1741 gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc ccgacttctt
     1801 accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg aacgagagct
     1861 accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg atccctgcat
     1921 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     1981 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     2041 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     2101 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     2161 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     2221 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc
     2281 gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc cgactatctg
     2341 gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg
     2401 ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag
     2461 gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg
     2521 ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat
     2581 gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg
     2641 atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg
     2701 ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt
     2761 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     2821 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     2881 ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag
     2941 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     3001 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     3061 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gacccctttc tggcgcgttt
     3121 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga
     3181 aaggcgctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag ggcgcaggct
     3241 cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg cggtgcccct
     3301 caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt ccacgctaga
     3361 tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc gcccgcgagc
     3421 gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg acgccgcagc
     3481 aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     3541 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     3601 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     3661 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     3721 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     3781 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     3841 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     3901 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     3961 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     4021 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     4081 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     4141 ttcggccagt gcgtcgagca gcgcctgctt gttcctgaag tgccagtaaa gcgccggctg
     4201 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     4261 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     4321 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     4381 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag acccctgatc
     4441 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     4501 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     4561 ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     4621 cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt cgactacgcc
     4681 atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc
     4741 accggggcga ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag
     4801 cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct
     4861 gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc
     4921 ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa
     4981 cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc
     5041 atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg
     5101 ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc
     5161 ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc
     5221 cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc
     5281 acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc
     5341 ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg
     5401 gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg
     5461 atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg
     5521 tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg
     5581 ctttggagcg gcgcagggca acgagccgat cgctgataga ggtttatagc ggcttcgttc
     5641 ccttccgcaa ccgtcagcat gatctgctcc acatgctcgc ggccatagtc tatcaccgcc
     5701 cgcagcagca taccggcgac ccctcggcct cgctgttcgg gctccacgaa aacgccggac
     5761 agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga cagcccaatg
     5821 atctcgccgt cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc
     5881 tccatgggct ttttctcctc gtgctcgtaa acggacccga acatctctgg agctttcttc
     5941 agggccgaca atcggatctc gccgaaatgc ctgccgttgc tagacattgc cagccagtgc
     6001 ccgtcactcc cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat
     6061 aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc cagcaggggc
     6121 gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa tccactaggc
     6181 gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc ggccagaggc
     6241 cggatagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg
     6301 ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata cgctggagcg
     6361 cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc
     6421 cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac
     6481 cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat
     6541 cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc
     6601 gccttgtttc gttcttgcgc tcttgctgct gttcccttgc ccgcacccgc tgaatttcgg
     6661 cattgattcg cgctcgttgt tcttcgagct tggccagccg atccgccgcc ttgttgctcc
     6721 ccttaaccat cttgacaccc cattgttaat gtgctgtctc gtaggctatc atggaggcac
     6781 agcggcggca atcccgaccc tactttgtag gggagggcgc acttaccggt ttctcttcga
     6841 gaaactggcc taacggccac ccttcgggcg gtgcgctctc cgagggccat tgcatggagc
     6901 cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat caccttacgg cgaaaaccgg
     6961 cagcaggtcg ggcggccaat cggccagggc caaggccgac tacatccagc gcgaaggcaa
     7021 gtatgcccgc gacatggatg aagtcttgca cgccgaatcc gggcacatgc cggagttcgt
     7081 cgagcggccc gccgactact gggatgctgc cgacctgtat gaacgcgcca atgggcggct
     7141 gttcaaggag gtcgaatttg ccctgccggt cgagctgacc ctcgaccagc agaaggcgct
     7201 ggcgtccgag ttcgcccagc acctgactgg tgccgagcgc ctgccgtata cgctggccat
     7261 ccatgccggt ggcggcgaga acccgcactg ccacctgatg atctccgagc ggatcaatga
     7321 cggcatcgag cggcccgccg ctcagtggtt caagcggtac aacggcaaga ccccggagaa
     7381 gggcggggca cagaagaccg aagcgctcaa gcccaaggca tggcttgagc agacccgcga
     7441 ggcatgggcc gaccatgcca accgggcatt agagcgggct ggccacgacg cccgcattga
     7501 ccacagaaca cttgaggcgc agggcatcga gcgcctgccc ggtgttcacc tggggccgaa
     7561 cgtggtggag atggaaggcc ggggcatccg caccgaccgg gcagacgtgg ccctgaacat
     7621 cgacaccgcc aacgcccaga tcatcgactt acaggaatac cgggaggcaa tagaccatga
     7681 acgcaatcga cagagtgaag aaatccagag gcatcaacga gttagcggag cagatcgaac
     7741 cgctggtcca gagcatggcg acactggccg acgaagcccg gcaggtcatg agccagaccc
     7801 agcaggccag cgaggcgcag gcggcggagt ggctgaaagc ccagcgccag acaggggcgg
     7861 catgggtgga gctggccaaa gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc
     7921 agagcgcccg gagcgcgtcg cgggggtggc actggaagct atggctaacc gtgatgctgg
     7981 cttccatgat gcctacggtg gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc
     8041 cactgacaac cgaggacggc tcgatctggc tgcgcttggt ggcccgatga agaacgacag
     8101 gactttgcag gccataggcc gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg
     8161 cgtcagggac gccaccaccg gccagatgat gaaccgggaa tggtcagccg ccgaagtgct
     8221 ccagaacacg ccatggctca agcggatgaa tgcccagggc aatgacgtgt atatcaggcc
     8281 cgccgagcag gagcggcatg gtctggtgct ggtggacgac ctcagcgagt ttgacctgga
     8341 tgacatgaaa gccgagggcc gggagcctgc cctggtagtg gaaaccagcc cgaagaacta
     8401 tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa cttcgggggc agattgcccg
     8461 gacgctggcc agcgagtacg acgccgaccc ggccagcgcc gacagccgcc actatggccg
     8521 cttggcgggc
//
LOCUS       DADLTD010000456         7500 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.guided.190, whole genome shotgun sequence.
ACCESSION   DADLTD010000456 DADLTD010000000
VERSION     DADLTD010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.guided.190"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            <1..651
                     /locus_tag="JN701_004328"
     CDS             <1..651
                     /locus_tag="JN701_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7366528.1"
                     /translation="HPIIRGTNKVTGQSILQRHIASFSHHGLPQGSDDALRYIAEHDD
                     ALAFACINRQMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNG
                     LVIIPGSRGDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTEL
                     GSRIICRDKQLIFEEAPQAYKSAESVVQCLGQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            648..1262
                     /locus_tag="JN701_004329"
     CDS             648..1262
                     /locus_tag="JN701_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAY7366529.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1319..2776)
                     /gene="pepD"
                     /locus_tag="JN701_004330"
     CDS             complement(1319..2776)
                     /gene="pepD"
                     /locus_tag="JN701_004330"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7366530.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3037..3495
                     /gene="gpt"
                     /locus_tag="JN701_004331"
     CDS             3037..3495
                     /gene="gpt"
                     /locus_tag="JN701_004331"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7366531.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3587..4831
                     /gene="frsA"
                     /locus_tag="JN701_004332"
     CDS             3587..4831
                     /gene="frsA"
                     /locus_tag="JN701_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7366532.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4889..5290
                     /locus_tag="JN701_004333"
     CDS             4889..5290
                     /locus_tag="JN701_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7366533.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5329..6384)
                     /gene="phoE"
                     /locus_tag="JN701_004334"
     CDS             complement(5329..6384)
                     /gene="phoE"
                     /locus_tag="JN701_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7366534.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6672..>7500
                     /gene="proB"
                     /locus_tag="JN701_004335"
     CDS             6672..>7500
                     /gene="proB"
                     /locus_tag="JN701_004335"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7366535.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     "
BASE COUNT         1818 a         1840 c         1959 g         1883 t
ORIGIN      
        1 caccccatta taaggggtac gaataaagta acggggcagt ctattttaca gcggcatatt
       61 gcctcgttct cgcatcatgg tttgcctcaa ggcagtgacg acgcgctacg gtatattgcg
      121 gaacatgacg atgcgctggc gtttgcgtgt attaatcgcc agatgatcgc tttgcgcata
      181 atgcaacaag ttaaggccac gggaagtccg gttctggatg tggcgcataa ctttgttagc
      241 gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta aaggtgccac gccggatgac
      301 aacggtctgg tgattattcc cggttcacgt ggtgattact cctggattgt acagcctgtt
      361 gcgaacgaaa aaacgctcca ctcgctggcg catggggcag ggcgtaaatg ggggcgcacc
      421 gagtgtaaat ggcgtctggc agcgaaatac acggtgacgc aactctcgcg gactgaactt
      481 ggcagtcgga taatttgtcg cgataaacaa ctcatctttg aagaagcgcc acaagcctat
      541 aaatcggctg aaagcgtggt gcaatgtctg gggcaggccg gtttaattat tcctgtcgcg
      601 cggctgcgtc cggtgctaac gctcaaaaac agtggaggga aaaaaggatg atcttgctac
      661 aactctcctc agcgcagggg ccggaagaat gttgtctcgc agtgaaaaag gcgctggaca
      721 ggctaattaa agaagctgcc cgacaagacg tcgcggtaac ggtgttggaa acagaaacgg
      781 gccgctactc tgacacgctg cgttcagctc tggtttctct ggatggcgat aacgcatggg
      841 cattaagcga aagctggtgt ggcactattc agtggatttg tccgagtccg tatcggcctc
      901 atcatgggcg caaaaactgg tttctgggca ttgggcgttt taccgctgat gagcaggagc
      961 aatccgatgc gatccggtat gagacgctgc gttcgtcggg gccgggcggt caacatgtca
     1021 ataaaaccga ctcggcggta cgcgccacgc atttggcatc cggtattagc gtgaaggttc
     1081 agtcagagcg tagtcagcat gctaacaagc ggctggcacg attgctgatt gcctggaagc
     1141 tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc gcagcggcga atgttccatc
     1201 accagattga acgtggcaac ccgagacgga catttacagg gatggctttt atcgaaggat
     1261 aatgaaggat gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt
     1321 acttcgccgg aatttctttc agcagttcag tcagcagtgt ccagtaatga cctacgcttt
     1381 cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat agttggcccg atagaaacca
     1441 tgtccatttc cggatacggt tttttgaaca gaccacattc caggcccgcg tggataatct
     1501 ggatgttcgg cgtcttgttg aacaggcgct gataggtttc acgtaccaga tgcatcaccg
     1561 gagaattagc gtccggctgc cagccaggat atgcgccttt cgcttcggtt ttcgcgccag
     1621 ccagtttacc cagcgaatcc agcatgctca ccacgtagtc tttaccgctg tcgatcagtg
     1681 aacggatcag gcagtgaatt tctacgttat tgtcagtcat ggtcaccaca ccgacgttca
     1741 gggaggtttc aaccacacct ttggctacat cggagttgcg gatcacaccg ttcggggtgg
     1801 cgttcagcag acgaataaag gtatcgcgag atttcgcaat cagggcagct ttatcgttcg
     1861 ctacagagtc cagcaacaag gccagatttt tctctttctc tgccagctcg tttttcagga
     1921 tctcctgata ggtattcacc agagatttca gtgcgtcgac tttatcagct gcgacagcaa
     1981 tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg aaatcgataa
     2041 ggcgcaggtc cagttcttct gcatgacccg ccaggaagcg caccagcagt ttgttggcat
     2101 tacccaggcc aacgtggatt tcaccgccgg agtgaccgcc tttcagacct tttaaggtta
     2161 acttgaaggt ttcaaaacca gctggaaccg cttcacgatc taaatgcagg ttggaggtga
     2221 agtcgatacc ccccgcacaa cccatgtaga tttcaccttc ttcttcggag tcggtgttaa
     2281 tcagaatatc agcctgcaac cagttgctct gtaagccaaa agcaccgtcc ataccggctt
     2341 cttcggtcat ggtcagcagc acttccagcg gaccgtgaac cacgttttcg tcagccagaa
     2401 ccgccagtgc agaggccata ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt
     2461 taacccattc gccatcaata taaggctgga taggatcttt cgtgaagtca tgcacggtgt
     2521 cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt ttacgatttt
     2581 ccatacctgc ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat
     2641 ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg
     2701 acgggtgcgg aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt
     2761 gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc
     2821 acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tttgagaata
     2881 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     2941 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     3001 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     3061 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     3121 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt
     3181 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     3241 cgagagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat agttattgat
     3301 gacctggtgg ataccggtgg tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac
     3361 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     3421 attccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     3481 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
     3541 aggcaggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     3601 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     3661 ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta aaaccatccc
     3721 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggtattg acccacgtga
     3781 aatcctcgac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg acgatgattt
     3841 atacgatact gtgattggct accgtggtgg caactggatt tatgagtggg ccacccaggc
     3901 gatggtgtgg caacaaaaag cctgtgcgga agaagatccg caactcagtg gtcgacactg
     3961 gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag gagatgacct
     4021 ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc
     4081 gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt
     4141 gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc
     4201 gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgtgat
     4261 gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca cccaggactc
     4321 cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg tggatcacac
     4381 tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct
     4441 tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag
     4501 tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg cgagtcgttt
     4561 ggggatgcat gatgcttccg acgaagcgtt acgcgtggag ctgaatcgct attcattaaa
     4621 agtgcaagga ttgctgggac gtcgctgccc aacgccaatg ttatcaggct actggaagaa
     4681 cgatccgttc agcccggaag aggactcacg cttaatcacc tcatcatctg ctgacggtaa
     4741 attattagag atcccattta acccggtgta tcggaatttt gacaaaggtc ttcaggaaat
     4801 caccggctgg atcgaaaaac gcttgtgtta aaaatttgct aaattttgcc aatttggtaa
     4861 aacagttgca tcacaacagg agatagcaat gacgttaccg agtggacacc cgaagagcag
     4921 attgatcaaa aaatttaccg cactaggccc gtatattcgt gaaggtaagt gcgaagataa
     4981 tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg
     5041 tgaattctgg ggctggtgga tggagctgga agcgcaggaa tcccgtttta cctacagcta
     5101 ccagtttggt ctgttcgata aagcaggcga ctggaagagt gttccggtaa aagatactga
     5161 agtggttgaa cgactggagc acaccctgcg tgagtttcac gagaagctgc gtgaactgct
     5221 gacgacgctg aatctgaagc tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt
     5281 aacggcgtga gtgaaatgtg ccggatgcat cacatccggc aatatctatt aaaactgata
     5341 cgtcatccca atcgcgacaa tatcatcatt attaatattc aatttgttat cgctatccag
     5401 ttggttgatt ttataatcaa caaatgctga catatttttg ttgaaataat acgtagcacc
     5461 gacgtcgata taattaacca gatcttcacc accgatgccc tcaatatctt tccctttcga
     5521 taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc
     5581 aaagttctgt gtcttattgg caaagccgcc agttattggc gtcattttgc gagtttcaga
     5641 atagaaagtt gccagataaa tattattggc atcgtatttc agacctgttg cccatgcttc
     5701 tgcacgcttg cctgtgccac ggctttgcag gttctgctcg ttggtgcgat ctgagttggt
     5761 ataggcccca ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa
     5821 gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat attgcagggt
     5881 taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct
     5941 ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggag tcgccaccaa attccggaaa
     6001 catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga
     6061 acccaaatct ttatatttca acccggcaaa agcgagacgc gttttttgct gtgcagtatc
     6121 actctctgct ttattaccgg caaactctgc ttcccaacga ccataaccag tcagttgatc
     6181 gttaatttgt gtttcgcctt taaaaccaaa acggatataa ctctggtcgc catctttact
     6241 gtcgttatca ctcatataat gcatggcttt aactttgcca tagacatcca gtttattacc
     6301 gtctttgtta tatatttctg cggcctgtac ggatgcagat gccacaatgc ccatcaccac
     6361 taatgccaga gtgctctttt tcattttcat tcctgatttt aattaacgcg cgaatattca
     6421 gcgggagagc cccgttgaaa acaggaaagt ttttaacctg agattgttaa agatatatta
     6481 cagattaata atattcttaa aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca
     6541 actgccgcta ggcttgctga tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt
     6601 ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat catttttcct aaaattgaat
     6661 ggcagagaat catgagtgac agccagacgc tggtggtaaa actcggcacc agtgtgctaa
     6721 caggcggatc gcgccgtctg aaccgtgccc atatcgttga acttgttcgc cagtgcgcgc
     6781 agttacatgc cgccgggcat cggattgtta ttgtgacgtc gggcgcgatc gccgccggac
     6841 gtgagcactt gggttacccg gaactgccag cgactatcgc ctcgaaacaa ctgctggcag
     6901 cggtagggca gagtcgactg attcaactgt gggaacagct gttttcgatt tatggcattc
     6961 acgtcgggca aatgctgctg acccgtgctg atatggaaga ccgtgaacgc ttcctgaacg
     7021 cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt tccggtaatc aatgagaacg
     7081 atgctgtcgc tacggcagag attaaagtcg gcgataacga taacctttct gcgctggcgg
     7141 cgattctggc gggtgccgat aaactgttgc tgctgaccga tcaaaaaggt ttgtataccg
     7201 ctgacccgcg cagcaatccg caggcagaac tgattaaaga tgtttacggc attgatgacg
     7261 cactgcgcgc gattgccggt gacagcgttt caggcctcgg aactggcggc atgagtacca
     7321 aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga caccattatt gccgcgggca
     7381 gcaagccggg cgttattggt gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg
     7441 cccaggcgac tccgcttgaa aaccgtaaac gctggatttt cggtgcgccg ccggcgggtg
//
LOCUS       DADLTD010000457         5716 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.guided.226, whole genome shotgun sequence.
ACCESSION   DADLTD010000457 DADLTD010000000
VERSION     DADLTD010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.guided.226"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            26..1159
                     /gene="blaEC"
                     /locus_tag="JN701_004336"
     CDS             26..1159
                     /gene="blaEC"
                     /locus_tag="JN701_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7366536.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1248..1781
                     /locus_tag="JN701_004337"
     CDS             1248..1781
                     /locus_tag="JN701_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7366537.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2095)
                     /locus_tag="JN701_004338"
     CDS             complement(1778..2095)
                     /locus_tag="JN701_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7366538.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2277..2423)
                     /gene="ecnB"
                     /locus_tag="JN701_004339"
     CDS             complement(2277..2423)
                     /gene="ecnB"
                     /locus_tag="JN701_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7366539.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2534..2659)
                     /gene="ecnA"
                     /locus_tag="JN701_004340"
     CDS             complement(2534..2659)
                     /gene="ecnA"
                     /locus_tag="JN701_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7366540.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2711..3277)
                     /gene="efp"
                     /locus_tag="JN701_004341"
     CDS             complement(2711..3277)
                     /gene="efp"
                     /locus_tag="JN701_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7366541.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3319..4368
                     /gene="epmB"
                     /locus_tag="JN701_004342"
     CDS             3319..4368
                     /gene="epmB"
                     /locus_tag="JN701_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7366542.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            4742..5611
                     /locus_tag="JN701_004343"
     CDS             4742..5611
                     /locus_tag="JN701_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366543.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         1529 a         1410 c         1384 g         1393 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 tgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg cagatgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtaccgc ccgcagaaga
      661 aaagaattac gcctggggat atcgcgaagg caaggcagtt catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaacc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
      961 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc
     1321 cgcgtggcgt gacagtagtg aataatttcg acgctaaacg ctatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gtctgcgtga tggcggcggc ctgaatgtca tcaataaagg ctataaccct gacagagaaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaacacgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt cattgcactt gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atagccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 aagccgtccc tactggtaac gatttcatcg cccaggcaag tagcgccata ctgacaatca
     1981 tcgccgtcac ggtaataaca ctcggcgtca aacgactaaa gccgtgggta tatttcaggc
     2041 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2101 gctccggaga attggggccg tccccggtga aaagacgcgt ttgcaggtcg tcctgcaaag
     2161 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt accttagtga
     2221 ctatcgcaga aaaaacaaac ggcacgacac agctgtcgta ccgttattgc ttattgctat
     2281 tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca
     2341 acgccacgcg tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc
     2401 gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc
     2461 tatagcaaat taataataga caacatcgcc aaaaatgacg gaaatctgat gatttttcgg
     2521 aagagaagaa aaattagctg gcagcgcgag agatggagtt gccgagatgt ttgatgtctt
     2581 cgccgaaacc gcgagcggtg ttacagcccg tgagcagcgt actggcaagc aaaacaagaa
     2641 cgataagtcg tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac
     2701 cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga
     2761 cttcgccgat ttgtacgaac agcggaacct taaccacagc gccagtagac agggtagccg
     2821 gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca
     2881 gttcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac
     2941 actctgcctg atccagcagc catttagcgt tatcgccaat tgcttttgca tcagcagaca
     3001 gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag
     3061 tcaggttcat atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt
     3121 ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac
     3181 ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa
     3241 gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat
     3301 tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat
     3361 tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat
     3421 atagacgcgg acgaaaaact gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt
     3481 gtgccccgct catttatcga tcgcatggag aaaggcaatc cggacgatcc tcttttgcgt
     3541 caggtactta cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac cgacccactg
     3601 gaagaacagc acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg
     3661 ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat
     3721 gccgaaaatc agggcaacaa gcgtaactgg cagactgcac ttgagtatgt tgctgcgcat
     3781 ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag
     3841 ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac
     3901 agccgtctgc cgattgtgat cccggcacgc atcacagatg cgctggttga acgcttttca
     3961 cattctacgc tgcaaatcct gctggtgaat cacatcaacc atgccaatga gatagatgaa
     4021 acattccgtc aggcgatggc taagttgcgc cgggtaggcg ttactttgct gaaccagagc
     4081 gttctgttac gtgatgtgaa cgataacgca caaacgctgg caaacctgag taatgcgttg
     4141 ttcgatgccg gcgtgatgcc gtattacctg catgtgctcg ataaagtaca gggcgcagcg
     4201 cattttatgg tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacattggtg
     4261 tcgggatatc tggtgccgaa actggcgcga gaaattggcg gcgaacccag caaaacgccg
     4321 ctggatctcc agctacgcca gcagttactg attaaacagg gaagttaaac attaaaattg
     4381 cctgatgcgc tacgcttatc aggcctacat cgtgcattga aattgaatgg atttgcaatg
     4441 aactgtaggc cggataaggc gtttacgccg catccggcaa gagcaacaaa cgcaagtaca
     4501 gcaacctgtc attaaactga gaaatttaat ctttgcccgt tgcataacct ttaattattc
     4561 atatgcattg ctgttgttac tttttatata gctaacacca ataattaacc tttcttttct
     4621 gtttatatag agtgagcacg gctatttatg ttattttaac aactattact accaagccca
     4681 tttatctggc ttataaatta aaattaatta tttaattgag aaatggttag ggagaaccta
     4741 catggccata agcatcaagg gtgttaatac cggtgttatt cgcaagagta ataatttcat
     4801 tgcactggcg ctgaaaatta aagaacctcg caataaagaa tcgttgttct ttatgtctgt
     4861 catggaactg cgcgacctgc tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact
     4921 ggatgctgct gcacgccttc agtatgagca agctcgcgat aaagtcatta agaaaatggc
     4981 agaaaacatc ccagaaattc tggttgatga gcttaaaaat gccgatatta atcgtcgggt
     5041 taacacgctg gaacttaccg ataatcaagg cgaaaatctt accttcgtat taacgctaca
     5101 tgacggtagc aaatgtgagt tagtcgtcaa tgagttgcag attgaaatgc tagcgcgagc
     5161 aatcattcat gcaattaata atgcggaaat gcgtgaactg gcattacgta ttacctcact
     5221 gttagatttc ctgccgttat atgacgttga ctgccaggaa aatggcaatc tggaatatga
     5281 cacctattcc caaccggaat ggaaacataa cttgttcgac cattatctgg cggtgcttta
     5341 ccgctttaaa gatgaaagtg gcaaagaaca atttagcggc gcagtggtga aaacgcgtga
     5401 agcaacgccg ggtaaagaag tagaggcgat cactcgccga atgcttgatt tcagcccacg
     5461 actgaaaaaa ctcgcgggtg tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa
     5521 cgcccaaccg ctaacccagg atcaatgctt acgcgcgctg catcacttac gtgttcaatc
     5581 caccagcaaa actgcaccac aggcgaaata atctcatctt tgctaatgcg aggctggagc
     5641 catccagcct gaaatgactg ataacgtgat cgttggtcat gccggatgcg gcgtaaacgc
     5701 cttatccggc ctacaa
//
LOCUS       DADLTD010000458         5484 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0380
            SAMEA1030001-rid11416113.guided.229, whole genome shotgun sequence.
ACCESSION   DADLTD010000458 DADLTD010000000
VERSION     DADLTD010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030001
            Sequence Read Archive: ERR047400
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942090.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942090.1
            Genome Coverage       :: 140x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 23:15:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,351
            CDSs (total)                      :: 4,298
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 309
            CDSs (without protein)            :: 309
            Pseudo Genes (ambiguous residues) :: 0 of 309
            Pseudo Genes (frameshifted)       :: 169 of 309
            Pseudo Genes (incomplete)         :: 80 of 309
            Pseudo Genes (internal stop)      :: 100 of 309
            Pseudo Genes (multiple problems)  :: 37 of 309
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030001-rid11416113.guided.229"
                     /strain="EG0380"
                     /db_xref="taxon:623"
     gene            58..297
                     /locus_tag="JN701_004344"
     CDS             58..297
                     /locus_tag="JN701_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY7366544.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(485..1462)
                     /gene="intI2"
                     /locus_tag="JN701_004345"
                     /pseudo
     CDS             complement(485..1462)
                     /gene="intI2"
                     /locus_tag="JN701_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1794..2267
                     /locus_tag="JN701_004346"
     CDS             1794..2267
                     /locus_tag="JN701_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY7366545.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2362..2886
                     /gene="sat2"
                     /locus_tag="JN701_004347"
     CDS             2362..2886
                     /gene="sat2"
                     /locus_tag="JN701_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY7366546.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2944..3732
                     /gene="aadA1"
                     /locus_tag="JN701_004348"
     CDS             2944..3732
                     /gene="aadA1"
                     /locus_tag="JN701_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7366547.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            3808..4305
                     /locus_tag="JN701_004349"
     CDS             3808..4305
                     /locus_tag="JN701_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366548.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4366..4737
                     /locus_tag="JN701_004350"
     CDS             4366..4737
                     /locus_tag="JN701_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366549.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            5147..5446
                     /locus_tag="JN701_004351"
                     /pseudo
     CDS             5147..5446
                     /locus_tag="JN701_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1499 a         1245 c         1321 g         1419 t
ORIGIN      
        1 cagcctcttc caactacaat aataagttaa ctatcctcca cccacaaata attacaaatg
       61 acccctttcc gctataactc cgatctcacc agcggctcgc tacaaacccg agagtgtcgc
      121 attattactg gcttgttact ccaagagctt gatgaagctg cctgggataa agccatgtat
      181 aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa gacgtatttc ttcagcgctt
      241 agaaaacgcc tagaacacct aagctctgac ttttgggctt ttgcgttttt atgttaggca
      301 ttgtctggca actggaggaa gtgtaatgac catgaatggt cactgtgatt gacagtatct
      361 tgcttaaact tatctgaaca atggtagaac tggctcatat ttatcccctc tatgattgtt
      421 tatctttttc accataaaaa gtatcagggg ctactcaggg ctacataaag atatttatct
      481 acttttactg attgataagt agcatcagtc catccgcagg actggtggtg ccggcaaaat
      541 gctgacccaa cacatgcgta tagatttgcg tggtcttaac atcgttatgc cctaagagtt
      601 cttgcacagt gcgaatatca cgccccgctt gtaatagatg cgtagcaaac gagtgacgaa
      661 atgtatgaca agtgacacgc ttgctaacga tgcctgcttt ttgtacggct gccttcaatg
      721 cctttcgcgc aacggagtca tgcagatgat ggcggcataa tttgccgtta tacgggtggt
      781 tgcagagcgt gctggaggga aagacaaaca tccacgccgc ttgtcgataa gcagaagggt
      841 atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc ttgtaagttg tcgtcttgct
      901 gaataagccg cgcttgctca atgagttatt ttattgctgg gattaggcgc gtgggcagta
      961 ggctgtttct gcttttccca cccttaccgt catgcacagt gatgcagcca ttatcaaaat
     1021 caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg caaacctgca ccatacagca
     1081 gcgtaaaaat aacttggttg cgagtatcca taacctgcaa aatgcgttgc acttcatttg
     1141 cagagataac agagggtagc cgtctaggct tgcttgcagg gatataatca atatcgccca
     1201 acggctgttg taaaaacctg ttgtacaaaa aagctagggc atttaaagcg attttctgcg
     1261 tgtttatggc tacatgtctg ctgtttgcta agctggataa aaacagcctg acctcttcac
     1321 tgcccatggt ctgaggatga cgttttttgt gaaacagaat aaaacgctta atccagtgca
     1381 ggtaagtttt ttcagttttc agcgcataac ctttttgtcg catatccgtg cgtatagaat
     1441 ttaaaaatgg actgttagac ataaaacgct ccttgtcttg caactgtctg cctatacagc
     1501 ctattctagc tgggatttaa aaaagtgcct gttttttacg cctagagatg cttgtttacc
     1561 ggtagagttt taatttaatg ctaaataaat taaaatgtta tgagttcttt gggtgagata
     1621 atgtgcatcg tgcaagcagg atagacggca tgcacgattt gtaataacag agtgtcttgt
     1681 atttttaaag aaagtctatt taatacaagt gattatatta attaacggta agcatcagcg
     1741 ggtgacaaaa cgagcatgct tactaataaa atgttaacct ctgaggaaga attgtgaaac
     1801 tatcactaat ggtagctata tcgaagaatg gagttatcgg gaatggccct gatattccat
     1861 ggagtgccaa aggtgaacag ctcctgttta aagctattac ctataaccaa tggctgttgg
     1921 ttggacgcaa gacttttgaa tcaatgggag cattacccaa ccgaaagtat gcggtcgtaa
     1981 cacgttcaag ttttacatct gacaatgaga acgtattgat ctttccatca attaaagatg
     2041 ctttaaccaa cctaaagaaa ataacggatc atgtcattgt ttcaggtggt ggggagatat
     2101 acaaaagcct gatcgatcaa gtagatacac tacatatatc tacaatagac atcgagccgg
     2161 aaggtgatgt ttactttcct gaaatcccca gcaattttag gccagttttt acccaagact
     2221 tcgcctctaa cataaattat agttaccaaa tctggcaaaa gggttaacaa gtggcagcaa
     2281 cggattcgca aacctgtcac gccttttgta ccaaaagccg cgccaggttt gcgatccgct
     2341 gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc ctgagcaggt ggcggaaaca
     2401 ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct atccgaccaa
     2461 ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat ctcggatgat
     2521 gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct tgtcgggaag
     2581 attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt tgtgtcgcac
     2641 acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta
     2701 agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc tgcctgcaat
     2761 ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta taaaactaga
     2821 cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc acaggatgac
     2881 gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca ggagttaaac
     2941 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     3001 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     3061 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     3121 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     3181 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     3241 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     3301 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     3361 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     3421 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     3481 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     3541 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     3601 tatcagcccg tcatacttga agctaggcag gcttatcttg gacaagaaga tcgcttggcc
     3661 tcgcgcgcag atcagttgga agaatttgtt cactacgtga aaggcgagat caccaaggta
     3721 gtcggcaaat aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     3781 tcaagcgtta gagagctggg gaagactatg cgcgatctgt tgaaggtggt tctaagcctc
     3841 gtacttgcga tggcatcggg gcaggcactt gctgacctgc caattgtttt agtggatgaa
     3901 gctcgtcttc cctatgacta ctccccatcc aactacgaca tttctccaag caactacgac
     3961 aactccataa gcaattacga caatagtcca tcaaattacg acaactctga gagcaactac
     4021 gataatagtt catccaatta cgacaatagt cgcaacggaa atcgtaggct tatatatagc
     4081 gcaaatgggt ctcgcacttt cgccggctac tacgtcattg ccaacaatgg gacaacgaac
     4141 ttcttttcca catctggcaa aaggatgttc tacaccccaa aaggggggcg cggcgtctat
     4201 ggcggcaaag atgggagctt ctgcggggca ttggtcgtca taaatggcca attttcgctt
     4261 gccctgacag ataacggcct gaagatcatg tatctaagca actagcctgc tctctaataa
     4321 aatgttaggc ctcaacatct agtcgcaagc tgaggggaac cactagtgtc atacgaacct
     4381 ccaagagacg gttacacaaa cgggtacatt gttgatgtca tgtatgacaa tcgcccaagt
     4441 aagtatccag ctgtgttcag aacgtacgtc cgaattccag actatgtcga actagataac
     4501 gcggaggagc tggccgacgc aatcattcga gaggttgagt taaaggaggc atggctcaca
     4561 agggtcttgg ggaactcagc cgatcctgac gcttctgtgg gcaagtggga ccggcgccta
     4621 gcgcagtgcg gtggtattga tgagatgtca agtggggtgc aggcaacata cttcagtcca
     4681 aacggtcagc tggtcggctt agtggcatac attgatgccg aatggatgga tgaatagctc
     4741 tgacgttacg attcaggtga gacaaccaga tgggcattgc gtacaagctc agttgccacc
     4801 gatgtggcta cgagtctgat cttctttacc taggccaagg tatggcgatg cttcctgagc
     4861 aaatagtcgg aacgtgcaca tgctgcgaca atctaacgac gatttctata aatgaaagct
     4921 acggagtatg ctcactttgt ggggctaagg atcgtgtgat atttacaagc tatcagcatg
     4981 aggcaaggcc aagaaatccc tgctatctag agagaagttt caaatatcaa tgcccaagat
     5041 gccatgcctt cagtatggag cctccggatc taccggagat gctttgggac taatgctccg
     5101 ctttgcctcg ggtatccgtt cgacctatgt ttaagaggaa acgcacgtgt ctgccgccaa
     5161 agctcttgcc tatgcacttt gtgacaagtt ggaaagcaaa aaagttgatg aagtcgtacg
     5221 aaatcttgtt tctagcggct ggaatattag ggaagtggcc tgggaagctt cgtcctttga
     5281 tcatgaaatg ccctactttg acacggggcg ggacttcgat attggatttc ctgacacgct
     5341 gtcatccaag attgagtttt tgaccgcgaa tggatcaatc gtccaagcac gtgctcgcct
     5401 gcacttcaag caggctctaa tttttgccaa ggcgtccaaa catgtgtaag cgtaaactga
     5461 ccgccgtatg tagccattaa gcct
//
